LOCUS       DATRSD010000001        58938 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRSD010000001 DATRSD010000000
VERSION     DATRSD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58938
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.001"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            94..2202
                     /locus_tag="VBD85_000001"
     CDS             94..2202
                     /locus_tag="VBD85_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7132585.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAAQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLNEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIDLGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(2296..3240)
                     /gene="mmuM"
                     /locus_tag="VBD85_000002"
     CDS             complement(2296..3240)
                     /gene="mmuM"
                     /locus_tag="VBD85_000002"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO7132586.1"
                     /translation="MGRFKELLESKKALILHGALGTELESRGCDVSGKLWSDKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAVREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIYKEGLKNFHRHRIELLL
                     DEGVDLLALETIPNAQEAEALIELLVEDFPQVEAYMSFTSQDGKTISDGSAVAGLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTDKPLVTYPNSGEIYDGASQSWQSSRDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSEHLT"
     gene            complement(3245..4621)
                     /locus_tag="VBD85_000003"
                     /pseudo
     CDS             complement(3245..4621)
                     /locus_tag="VBD85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(4751..5401)
                     /locus_tag="VBD85_000004"
     CDS             complement(4751..5401)
                     /locus_tag="VBD85_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO7132587.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFNFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDRFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKKTNYIISPWQMDRFHKVINFVRIKK"
     gene            complement(5413..6062)
                     /locus_tag="VBD85_000005"
                     /pseudo
     CDS             complement(5413..6062)
                     /locus_tag="VBD85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6154..6783
                     /locus_tag="VBD85_000006"
     CDS             6154..6783
                     /locus_tag="VBD85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7132588.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTLYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQATFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKNKKELCSSLNSYCKALFVINSQIITQIK"
     gene            complement(6764..7360)
                     /locus_tag="VBD85_000007"
     CDS             complement(6764..7360)
                     /locus_tag="VBD85_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO7132589.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7371..8597)
                     /gene="clpX"
                     /locus_tag="VBD85_000008"
     CDS             complement(7371..8597)
                     /gene="clpX"
                     /locus_tag="VBD85_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO7132590.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKIIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8815..9309)
                     /locus_tag="VBD85_000009"
     CDS             complement(8815..9309)
                     /locus_tag="VBD85_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7132591.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9389..10228)
                     /locus_tag="VBD85_000010"
     CDS             complement(9389..10228)
                     /locus_tag="VBD85_000010"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO7132592.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLVVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10277..11554
                     /locus_tag="VBD85_000011"
     CDS             10277..11554
                     /locus_tag="VBD85_000011"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO7132593.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDRLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKEYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11556..12839
                     /locus_tag="VBD85_000012"
     CDS             11556..12839
                     /locus_tag="VBD85_000012"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO7132594.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHAKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEGFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSKWSYKVDDNFLEGTLTLPMPVATKGGSIGINPSVQ
                     LAHDLLGRPNAKELASIVLSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12931..13380
                     /locus_tag="VBD85_000013"
     CDS             12931..13380
                     /locus_tag="VBD85_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO7132595.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLEILTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13373..14029
                     /locus_tag="VBD85_000014"
     CDS             13373..14029
                     /locus_tag="VBD85_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO7132596.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYSSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            14116..15030
                     /locus_tag="VBD85_000015"
     CDS             14116..15030
                     /locus_tag="VBD85_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7132597.1"
                     /translation="MKSAYIFFNPKSGKDEQALAQEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFVGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            15131..15985
                     /locus_tag="VBD85_000016"
     CDS             15131..15985
                     /locus_tag="VBD85_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEO7132598.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYKGPYTIPILIDKTD
                     NRIVCKESADMLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINNNFQSLMYKLA
                     FLDKQTDYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMVKWEK"
     gene            complement(16031..16249)
                     /locus_tag="VBD85_000017"
     CDS             complement(16031..16249)
                     /locus_tag="VBD85_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO7132599.1"
                     /translation="MIKLYYEDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKNIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(16317..17312)
                     /locus_tag="VBD85_000018"
     CDS             complement(16317..17312)
                     /locus_tag="VBD85_000018"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO7132600.1"
                     /translation="MTNRKDDHIKYALKYQSPYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMATGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPLPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRAV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(17309..18301)
                     /locus_tag="VBD85_000019"
     CDS             complement(17309..18301)
                     /locus_tag="VBD85_000019"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO7132601.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNIPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQASLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTNYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(18294..19238)
                     /gene="mvaD"
                     /locus_tag="VBD85_000020"
     CDS             complement(18294..19238)
                     /gene="mvaD"
                     /locus_tag="VBD85_000020"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO7132602.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTTLGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNKSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTDLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(19220..20098)
                     /gene="mvk"
                     /locus_tag="VBD85_000021"
     CDS             complement(19220..20098)
                     /gene="mvk"
                     /locus_tag="VBD85_000021"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO7132603.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(20214..21443)
                     /locus_tag="VBD85_000022"
     CDS             complement(20214..21443)
                     /locus_tag="VBD85_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7132604.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSDSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLASFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(21443..22129)
                     /locus_tag="VBD85_000023"
     CDS             complement(21443..22129)
                     /locus_tag="VBD85_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7132605.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAISANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(22131..22757)
                     /locus_tag="VBD85_000024"
     CDS             complement(22131..22757)
                     /locus_tag="VBD85_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7132606.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKAIRDSIGVRIICGFVNDIYQIVERIKSFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYIEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEMASVDLTMQTIRQLIESGTKKE"
     gene            complement(22865..24619)
                     /locus_tag="VBD85_000025"
     CDS             complement(22865..24619)
                     /locus_tag="VBD85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132607.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(24609..26426)
                     /locus_tag="VBD85_000026"
     CDS             complement(24609..26426)
                     /locus_tag="VBD85_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132608.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIWGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAQKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGAIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MILGESGREELSDEEIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHELKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(26473..26913)
                     /locus_tag="VBD85_000027"
     CDS             complement(26473..26913)
                     /locus_tag="VBD85_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7132609.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            27182..29254
                     /locus_tag="VBD85_000028"
     CDS             27182..29254
                     /locus_tag="VBD85_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7132610.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSAFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYAIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPAISKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VVAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSVPNNKP
                     KIYVTMKMVNETITQNDGTHSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(29291..29701)
                     /locus_tag="VBD85_000029"
     CDS             complement(29291..29701)
                     /locus_tag="VBD85_000029"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7132611.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLIGCRSTQRYRHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(29771..31120)
                     /gene="gdhA"
                     /locus_tag="VBD85_000030"
     CDS             complement(29771..31120)
                     /gene="gdhA"
                     /locus_tag="VBD85_000030"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO7132612.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASEATDEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            31288..31797
                     /locus_tag="VBD85_000031"
     CDS             31288..31797
                     /locus_tag="VBD85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO7132613.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLIQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(31838..33607)
                     /locus_tag="VBD85_000032"
     CDS             complement(31838..33607)
                     /locus_tag="VBD85_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132614.1"
                     /translation="MRKKSVFLRLWSYLTRYKATLFLAIFLKVLSSFMSVLEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNHKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNGISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLNVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(33612..35351)
                     /locus_tag="VBD85_000033"
     CDS             complement(33612..35351)
                     /locus_tag="VBD85_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132615.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMAYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVVLYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNAEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDFTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(35418..35891)
                     /locus_tag="VBD85_000034"
     CDS             complement(35418..35891)
                     /locus_tag="VBD85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7132616.1"
                     /translation="MIIRPIIKNDDQAVAQLIRQSLRAYDLDKPDTAYSDPHLDHLTS
                     YYEKIEKSGFFVIEERDEIIGCGGFGPLKNLIAEMQKVYIAERFRGKGLATDLVKMIE
                     VEARKIGYRQLYLETASTLSRATAVYKHMGYCALSQPIANDQGHTAMDIWMIKDL"
     gene            complement(35888..37756)
                     /locus_tag="VBD85_000035"
     CDS             complement(35888..37756)
                     /locus_tag="VBD85_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7132617.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLHPDSGNVSIGETIRVGYFSQQLNNID
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDIMILENTITRIENDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSELDT"
     gene            complement(37767..38975)
                     /locus_tag="VBD85_000036"
     CDS             complement(37767..38975)
                     /locus_tag="VBD85_000036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO7132618.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHASLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(39160..40008)
                     /locus_tag="VBD85_000037"
     CDS             complement(39160..40008)
                     /locus_tag="VBD85_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7132619.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(40008..40388)
                     /locus_tag="VBD85_000038"
     CDS             complement(40008..40388)
                     /locus_tag="VBD85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO7132620.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQMAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(40460..40993)
                     /locus_tag="VBD85_000039"
     CDS             complement(40460..40993)
                     /locus_tag="VBD85_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132621.1"
                     /translation="MTMKTIEEIYKNYPNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            complement(41044..41538)
                     /locus_tag="VBD85_000040"
     CDS             complement(41044..41538)
                     /locus_tag="VBD85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7132622.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLN
                     FSKR"
     gene            complement(41727..43691)
                     /locus_tag="VBD85_000041"
     CDS             complement(41727..43691)
                     /locus_tag="VBD85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7132623.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAVVSGVLFGLFRKTK"
     gene            complement(43688..44599)
                     /gene="pfkB"
                     /locus_tag="VBD85_000042"
     CDS             complement(43688..44599)
                     /gene="pfkB"
                     /locus_tag="VBD85_000042"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO7132624.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            complement(44596..45405)
                     /locus_tag="VBD85_000043"
     CDS             complement(44596..45405)
                     /locus_tag="VBD85_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7132625.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKAIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            complement(45470..46705)
                     /locus_tag="VBD85_000044"
     CDS             complement(45470..46705)
                     /locus_tag="VBD85_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7132626.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPYDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGIQGTFDGSDSILRFKQNFNGYIIRKMGTFNYY
                     PSPFKYKGIQLLKKVLKRN"
     gene            complement(46849..48483)
                     /locus_tag="VBD85_000045"
     CDS             complement(46849..48483)
                     /locus_tag="VBD85_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO7132627.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            complement(48695..49618)
                     /locus_tag="VBD85_000046"
     CDS             complement(48695..49618)
                     /locus_tag="VBD85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO7132628.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            complement(49631..50701)
                     /locus_tag="VBD85_000047"
     CDS             complement(49631..50701)
                     /locus_tag="VBD85_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7132629.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            complement(50838..51830)
                     /locus_tag="VBD85_000048"
     CDS             complement(50838..51830)
                     /locus_tag="VBD85_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7132630.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKIDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPICLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            complement(51811..52566)
                     /gene="trmD"
                     /locus_tag="VBD85_000049"
     CDS             complement(51811..52566)
                     /gene="trmD"
                     /locus_tag="VBD85_000049"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO7132631.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIGKIDAQKARVILLDPAGRTFDQNFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            complement(52553..53071)
                     /gene="rimM"
                     /locus_tag="VBD85_000050"
     CDS             complement(52553..53071)
                     /gene="rimM"
                     /locus_tag="VBD85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO7132632.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQELALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAENQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            complement(53134..53205)
                     /locus_tag="VBD85_000051"
     tRNA            complement(53134..53205)
                     /locus_tag="VBD85_000051"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53170..53172),aa:Glu,seq:ttc)
     gene            complement(53214..53285)
                     /locus_tag="VBD85_000052"
     tRNA            complement(53214..53285)
                     /locus_tag="VBD85_000052"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53251..53253),aa:Gln,seq:ttg)
     gene            complement(53393..54904)
                     /locus_tag="VBD85_000053"
     CDS             complement(53393..54904)
                     /locus_tag="VBD85_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7132633.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNMLQFLAYFIALQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYNLKDLSEEYYYFYILVNLAWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NVNEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDRGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLKNDSGVAIQDILEIGMNNKQYHELLLQAIIEKRDQKVFEYISKMKHKIL"
     gene            complement(55036..55278)
                     /locus_tag="VBD85_000054"
     CDS             complement(55036..55278)
                     /locus_tag="VBD85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO7132634.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            complement(55288..55560)
                     /gene="rpsP"
                     /locus_tag="VBD85_000055"
     CDS             complement(55288..55560)
                     /gene="rpsP"
                     /locus_tag="VBD85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO7132635.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            complement(55689..56936)
                     /locus_tag="VBD85_000056"
     CDS             complement(55689..56936)
                     /locus_tag="VBD85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7132636.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENMYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            complement(56951..57661)
                     /locus_tag="VBD85_000057"
     CDS             complement(56951..57661)
                     /locus_tag="VBD85_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132637.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVPPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            complement(57664..58908)
                     /locus_tag="VBD85_000058"
     CDS             complement(57664..58908)
                     /locus_tag="VBD85_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO7132638.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSLPAMELSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLNSNKKSEVK"
BASE COUNT        18978 a        11716 c         9273 g        18971 t
ORIGIN      
        1 ttaaatattt aaattagacc tctctatatg tattaaagta taggaatcaa taattaactt
       61 tcaaaaatta aaatcaattg aggtatttaa tatatggcaa ataataattt ttataatcgt
      121 gacccttttg gaaatatgga tgatattttt aattctctaa tgggaaatat ggggggctat
      181 aatagtgaga ataaacgata cttaatcaat ggacgggaag ttactcctga agaattcagt
      241 caatatcgtc aaactggaaa actccccggt caagagctaa ataaccaaaa tactcctaca
      301 aaccaagtct ctgcagatag tgttttgact aaattaggaa ctaatttaac cgatcaagca
      361 cgtcagcatt tgctagatcc agttattgga cgaaacaaag aaattcagga aactgcagaa
      421 attctagctc gtcgtactaa aaataatcct gttctagttg gtgatgcagg tgttggtaaa
      481 acagctgtta ttgaaggttt ggcacaagca attataaatg gtgatgtccc tgctgctatc
      541 aaaaataaag aaatcatttc gatagatatc tctagccttg aagctggcac ccaatatagg
      601 ggtagttttg aagaaaatat tcaaaatatc ataaaagaag ttaaggaaac aggaaatatt
      661 attctcttct ttgatgaaat acaccaaata ttaggagccg gttcaactgg tggtgatagt
      721 ggctctaagg ggctcgcaga tatacttaag cctgcacttt cacgtggtga attaacggtt
      781 ataggtgcaa ctactcaaga tgaatatcgt aacaccattc tcaagaatgc tgccttagca
      841 cgccgcttta acgaagttaa agtcaatgca ccttctgcac aagatacttt caatatcctt
      901 atggggataa gaaacctcta tgagcaacat cataatgttg ttttacctga ctctgttttg
      961 aaagcagctg tagatttatc tattcaatac ataccacaaa gaagtctccc tgataaagca
     1021 attgacctta ttgatatgac tgctgcacac ctagctgcgc aacatcccgt aactgacctt
     1081 aaatccttag aaaaagaaat cgctgcgcaa agagacaagc aagaaaaagc tgtcaatact
     1141 gaagattttg aagaagctct taaggtcaag acacgcattg aagagttaca aaaccagatt
     1201 gataatcata ccgaaggaca aaaagtcact gcaactatca atgatatcgc tatgtcaatt
     1261 gagcgtctaa ctggagttcc tgtatcaaat atgggtgcta gtgatattga acgcttaaaa
     1321 gaacttggca atcgtctaaa aggtaaagtc attggtcaaa atgatgctgt tgaggcagtt
     1381 gcaagagcta ttcgccgaaa tcgtgctggt ttcgatgatg gtaatcgtcc gattggtagc
     1441 tttctatttg ttggaccaac tggagttggt aaaactgagc tagcaaagca attagctttt
     1501 gatatgttcg gatcaaagga tgctatcgtt agacttgata tgtctgaata taatgatcgt
     1561 actgcagttt ctaagctaat tggagcgact gctggttacg ttggttatga cgacaatagt
     1621 aatacactaa cagaacgtat ccgtcgaaat ccttattcta ttgttcttct tgatgaaatc
     1681 gaaaaagctg acccgcaagt tatcacgctt ctccttcaag ttctagatga tggtcgttta
     1741 actgatggtc aaggaaacac tattaacttt aaaaatactg ttatcattgc aacctcaaat
     1801 gcaggttttg gaaacgaagc atttacaggt gacagcgata aagacttgaa aattatggaa
     1861 cgaatttctc catatttccg tccagaattt ctaaatcgtt tcaatggtgt tattgaattc
     1921 tctcacctaa gcaaagatga cttaaacgaa attgtagatt tgatgcttga tgaagttaac
     1981 caaacaattg gcaaaaaagg aattgacctt gtggtagatg aaaatgttaa atcacactta
     2041 attgacctgg gttatgacga agcaatggga gtacgtccat tgcgccgtgt catcgagcaa
     2101 gaaattcgag atcgcatcac agactactat ctcgatcata cagatgttaa acacctaaaa
     2161 gctaatttgc aagatggcca aatcgtcatt tctgaaagat aaaaacaaaa cccgtttgga
     2221 agaatccaag cgggttttct agtggattga aaaagggaat cagaacttat caaaatcttc
     2281 tttaattcac tttacttatg tcaaatgttc tgagaggtca gcaatatcag ctggtctagt
     2341 tcggcaacaa cctccgacaa cttgggcgcc aagtttttgc cagtcacttg tattttcaag
     2401 tagggtgtgt gaatgatctc ggctagattg ccaagattgg cttgccccat cataaatctc
     2461 tccagagttt ggataggtca caagaggctt gtccgtctgc tctgctattg cttgcaagaa
     2521 atcagcaact aacgatggac ttgaacagtt aatgcccagc gccacgacct gtgggctaac
     2581 atcaatagct ttcgccaagc ctgcaacagc actaccatct gagatggtct taccatcttg
     2641 agaggtgaat gacatataag cctcaacctg agggaaatcc tcgacaagta gctcaataag
     2701 agcctcagcc tcctgagcat ttggaatggt ttcaagtgcc agaaggtcaa caccctcatc
     2761 taaaagaagc tcaatacgat ggcgatggaa atttttgaga ccttccttat aaatatcgta
     2821 gagccctgtg tactcagaac cgtccgccaa aaaggcagcg tagggaccaa catccccaga
     2881 aatcaaagga tagattcttt ctgatttttc ttcttttgtc aaacttttcc aaacttgctc
     2941 acgcacagct tttgccaact gaacagtcaa gcgaatcaag tcttctgcct gactttcaga
     3001 gacaccaact tgagctagcc cttgtaaagt cgcctgataa gttgatgtcg tcacaatatc
     3061 agcgccagca cgaatataat cttcatgaat ggtctgaata gctgctggat cctcaatcag
     3121 atatttgtct gaccaaagtt taccagaaac atcacaacct ctactctcaa gctcggtccc
     3181 caaggcaccg tgtaggatga gagctttttt actctctaaa agttctttaa atcttcccat
     3241 aagatcacca tgtcttgtaa cgtagtttgt gataaatata acataaaata ataaatggaa
     3301 caccgaagta aagcccagca cgttgtgagc tatcccaagc aatccccaca atcgaaatca
     3361 aaagcaaaac gattgtgata taagggagaa ctggtgtaaa gggtgttttg taagacaaat
     3421 cttctagtcg attctctttc aagaattcct tgcggaaatt gatttgtgcg accggaatgg
     3481 ctagccatac gacaacaaca gcaaagcccg cgattgaaac caaggctaga taaactgtgt
     3541 ctgcagcgta aatacttgaa aagagcgaca gcactgctcc tgccattgac aagagaagag
     3601 cacgcattgg gacaccgtgt ttattgattt tcacgacaga ttttgacaac ataccttcat
     3661 tggcaaggga ccagagcata cggcttgatg cgtagagacc tgagttacca gcagacagga
     3721 tggctgtcaa gataacaaag ttcatgatat ccgccgcaaa agggactccc atcttgtcaa
     3781 agacatcaac gaatggtgct gtggatacgc ctgcctcttt cattggcaat agcgaagcta
     3841 ggacaacaat tgtcagtaca aagaaaacaa ccaagcgacc gattgtcgtt ttaatagccc
     3901 ttggcacagc ttcttttgga ttatccgttt cacctgccgc aataccaatc aactcagtac
     3961 cagagaaggc atagttaaca gccaacatga ctgagacaac tgcaacgata ccatttggaa
     4021 agtcaccatt ggcagtcaga tgagtgaaaa gaatagcttt gtgctaacct tcaaaggaaa
     4081 ctagaccaaa catagcacct aagcccaaga taataaagat aatgatagca ataaccttaa
     4141 tacttgagaa gaaagactct gcttctgcaa aaaagcgcac actgagagca tttaagccaa
     4201 aaatcacaag ggcaaagaaa gaagcaaatg cccaagccgg cacatctggg aaccagcgct
     4261 gcatgagcat ggcagcacct aaaaattcag ttcccaaggc gaccgtccaa caaatccaat
     4321 atagccaggc aacagtaaaa cctgtgccag gactgataaa cttagtggca taagtgtgga
     4381 atgaccccgt caccggcatg gcaactgcca attcaccaag tgatagcatg accaaataaa
     4441 ccacaacggc accaatcaaa taagacagca cagctccaag cggaccagcc tgtgcaatgg
     4501 tataacctga actcaagaaa agcccagtcc cgataacacc acctagagat aacataaaga
     4561 gatgacgact ggtcatctta cgctggaatt taccttcatc ttcataacga tgattttcca
     4621 taacaacctc caagaaacta atacttttat cctaccacaa tctagtcgta ttggggaata
     4681 ggtattagct atcatatatg ataaagaaaa actattacaa aaaatcagcg aatcataaat
     4741 agcggtaacc ttattttttt atccttacaa aattaatgac cttatgaaaa cggtccattt
     4801 gccaaggact aataatataa ttggtcttct tatccttcaa tagctgtaca ggtgcaataa
     4861 tactagttag tacacagaca atcaacatga taaaaccacc tagccaatct ccccaagtga
     4921 gcattccttc tgcactagct tgatagatgt atgcgcaagc tattggtact aggagaaata
     4981 ctgtcgttgc tagaccagga ttataccaag ttttaagttt aatattcatc tgaaaaccat
     5041 gacccaagag ctgcgttaga ctaaagaaca tgactcctaa tccaagccaa taagcttgag
     5101 gaaaagcaat actagcaatg taaagcaacc aagcaatagt attaaccaac ataatagact
     5161 gagtattgcc tggaaaacga tccatcagct cttcttcatc ataaacaaca tagttaatga
     5221 taggtgatgc cccaccggga aactgatatt cttcaaattg atgcgctaga agtgccataa
     5281 agttaaaata caatagatag tgaagcattg accagtcagg tctaaaaaca agcaatatta
     5341 ctgctaaaat aataaataaa attgctccaa aacgatacca gtaatttcta aaatatgaca
     5401 tgataatacc tcctatttaa actgtgtttc acgaaaatgt gttaaaaatg gttttgagcg
     5461 tgaaagatca acatcactaa atgcgtaatt tgaatgtcga cctaaacgct tatagattgg
     5521 ggaacgaaag gctataaagt agttaagaac aatccatatc agcctaaaaa ccaatcaagt
     5581 ccagaataca acttccaatc atgagctaaa taatagacag aaacagggac aagacctact
     5641 aaggtcgtaa gaagtcctgg attataccac ttcttcaagg aaagattaaa aaagaaaaga
     5701 tgcatggcaa gttctgcaaa tgcaaaaatc atgactgcaa gtgttaaaaa tggtatattg
     5761 ggacaaaaaa tcggcaaaag ataaacaatc actgcaaacc actgattccc gaaaaaggct
     5821 gatagatggt ttaagtccca cttactagta tcagtttcaa ccgatttaag ttcaactagc
     5881 atagcgataa gcggaaaacc tccagggaaa ccaaattctt cgaaaaagtg taattaaata
     5941 aaaagtcccg atataagtag tgactgcact cgtaaatccc aatttccaaa aaataatgct
     6001 aaggcaaaaa gagaggcaag atagggacaa aagaggtacc attttgttat caatgttttc
     6061 ataagatgac ctccaagatt tgtaacattt gataccatat agtataatca agtgaaccta
     6121 taatatcaat acctttggag gttttatgca caaatgactg aaagtgtgac agttaataaa
     6181 aagagaataa aacaaatcac tcaaacacaa gaggatatct taacagcttt tggccaactt
     6241 ttggaagagt atccctatga gaaaattcag atcagccaaa ttgccaagaa atctggctat
     6301 gctagaagga ccctttaccg acactttgac agtcgagatg acctacttac ccttttcatc
     6361 gaaagactaa cattaaatct ctttaagcaa cttggtcaat tggaacaagc tacttttagc
     6421 caagttttcc agaatttttt tagttattgg tctgattata aatcccttct tctcattctt
     6481 agaaagaatg acctactccc tcaatttcaa caatcctggt tccgacatat tgatttgata
     6541 gagttaggtc gtggtgattt atcttccaat acctatgctc agcgatttgc gattggtggt
     6601 attttcagtg ttctaattga gtggattcat caagactgcc aaacaagtat cgaagaatta
     6661 actcaagttt ccttcgatat tatcaatcat ttaaaaaata aaaaagagct ttgcagtagt
     6721 ctcaacagct actgcaaagc tctttttgtc ataaactctc aaattataac tcagataaaa
     6781 tagtatccca tgattcatca agacctgtgc ggtcaacaga tgaaaagacg atgaaatggt
     6841 cctttttatc aaagtttaat ttcttcttaa tagacgactc atgtttattc cacttgccac
     6901 gaggtatttt atctgccttt gtcgctacaa taattactgg aatttcatag tacttaagaa
     6961 attcatacat ttgaatatcg tccgcacttg gatcatgacg aaaatcaact aaactaacaa
     7021 caacacgaag gttatcacgt gttacaaggt actcttcaat catcttgccc cacttagcac
     7081 gctcagtttt agacacctta gcgtagccat atccagggac atcgacaaaa cgcagtttgt
     7141 catcaatgtt ataaaagttg agtagttgtg tcttacctgg cttactcgac gtacgcgcta
     7201 ggtttttacg accaagaagc gtgttgataa agcttgattt accaacattt gaacgaccag
     7261 cgagagcaac ttctgggaga tcatcctgag gataatgtga cttgttagct gcactcaata
     7321 aaatagacgc attatgagta tttaaaaatt cttctgccat ctctatctcc ctaagccgtc
     7381 tctaaaacag gcttgtcagt accctcaaca gcagcctttg tgatacgaac ttttgttaca
     7441 tcttcttggc ttggaatttc aaacatgata tcaagcatcg tttcttcaat aatagaacgt
     7501 aaaccacgtg caccagtctt gcgctcgata gccttatcag cgatagccaa tagagcatcc
     7561 tgatcaaatt ccaattctac accatcataa gataataagg tttggtattg tttaaccaaa
     7621 gcattgcgtg gttctgtcag aatacgaacc agatcttctg cagtaagaag ttctaacgct
     7681 gcaactactg gtaaacggcc aataaactct ggaatcagtc caaacttttg aatatcctca
     7741 gaaattatct cttgcatata agaagcgttg tcatcaattt tgcggcttgt ctgtccaaaa
     7801 ccaataattt tttcgcctaa acgttgcttc acaaggtctt caataccatc aaaagcacca
     7861 ccgacaataa aaaggatgtt cttggtatta atttgaatca tttcttggtt aggatgttta
     7921 cgcccaccct gtgggggaac acttgctacc gtaccctcaa taattttaag aagggcttgc
     7981 tgtacacctt caccagatac atcacgtgtg atagaaacat tttcgccttt cttagcaatt
     8041 ttatctattt catcaacgta gataatacca cgctcagcac gttcgacatt ataatcagca
     8101 gcttgaatca atttaagaag aatattctca acatcttctc caacgtatcc tgcttcggtc
     8161 aatgaagtcg catctgcaat agcaaacggt acattaaggc ttttagctag tgtttgtgct
     8221 aagaaggttt ttcctgagcc agttggacca atcatcaaaa tgttggattt ttgcaaatct
     8281 acatcatcgt cactactctc ggtataacta acacgcttgt aatgattgta gacagcaact
     8341 gctaaagcac gtttagcacg atcttgccct acaacatatt gatttaatat ttctaatagt
     8401 tccttaggtt ttggtacttc tgctaaatca gcaagtactt cctcagctaa ttcttcctta
     8461 ataatttctt gtgataaggc cacacattca ttacaaatga aaacaccatt acctgcaata
     8521 atttttttta cttcatcttg gcttttgcca caaaatgaac aatagacatt catatcgtta
     8581 ttacgattcc cagccatttt ttcctcaact tttctattca tttaatttaa aataaggttc
     8641 cataaaaggc atggattgtg ttgacaaggt tagtgaaagc attcatcaaa aaaaccgttc
     8701 ctaatccata tctttttttc tttatagcag gaattgcaca taaaacacaa atcacagcta
     8761 aaaatgcggt aaagacccca aaaaggctac gttgcattga atagtattcc ttccttattt
     8821 ttcaaaatat gtcacagtaa aagcatgagc attctgctca tccttttcaa aataatctct
     8881 agagataact ttaaactcag atagattaac atcaggaaaa taggtatcac ctttaaattt
     8941 tccatgaact ttagttttta tgattgcttc acaataaggc agaaatgctt tataaatact
     9001 tgcaccacct acaataaata aggtcttatt atgttcctga aaccatttta taacttgttc
     9061 aacactattt aggactgtca ctccatctgc ttggaattgt tcatcttttg ttaagatgat
     9121 tgtctctcta ccaggtaaaa cacgacggtt cattccatca aaggtctttc gtcccataag
     9181 caaagcctgc cccatggtcg tttctttgaa gtgatgtaac tctttaggaa gcctccatgg
     9241 taaaccgcca ttaactccaa tcaaatggtc ttcatcttca gcccaaatgg caattatttg
     9301 ctttgtcatg acttttttcc tcactaactt atctctagat attctaacaa attttacctc
     9361 aaattgcttg ttaaaaaaca tgagatagtt agattgctaa atcaaaacgt aattgaggtt
     9421 taattgggtc ataatcaact aacgcaaaat catctggttt aatatcaaaa aagtctgttc
     9481 catctggaac attcaatact agtttggggt tacactcaga cggttggcgt tttaagagtt
     9541 cctgagcttg ttcaaactgg ttatcataaa tatgaagatt attgataaaa tagaaaaact
     9601 tcccaactct ccaaccaaaa tgctttgcaa tcatcatctg aagtgctaca tactgcatag
     9661 cattaatatg atgtgcaact aacatgtcat tagaacgttg tgttaatgtg gcatccaaat
     9721 aaagctcacc attaacgcgt ctcacatcaa acatcgtttg aaaagcacaa ggtagtaggc
     9781 cttctgtttc ttcaaatgct tcatagtccc ataatgaaat aacattacga cgattccaag
     9841 ggttatcttc aagctgtttt aagagttttg atataatatt atgtttttta acaactgcac
     9901 cgtagcgctg acctatcgtg ccagtatggc caacttccca atcattccag tatgtcacac
     9961 catatttatc attcaaaact actaaatcat ttgtctgatc ttgataaatc cagaaaattt
    10021 ctttaatagc agattttatt ggaattggac gtagagttgt aataggaaat tcaccctttg
    10081 ataaatcata ctctgcaaag gcacctgtaa tatacttaga attagccatt tccccatttt
    10141 tatatctagg tctggcttgc tcgctaaata caccttcaga cataattttc gtgatattgt
    10201 ctttaaataa taaatcggct tttgtcatga gatacctcca aagtcttatt ttatcagatt
    10261 tttaaaagat attctagtga gaaacaagac tttgaaggtt aaagcttttt ttcatatttt
    10321 tagcttcttt atggtaaaat ggaatttagc aaaaatgact tttattcaga aaggtttacc
    10381 aatgaaaata ggaattgata agattggttt tgcaaccagt caatatgtcc ttgaaatgac
    10441 tgatttggct attgctcgcc aagttgaccc tgaaaaattc agcaagggat tattgttaga
    10501 ttcacttagt attacgccag ttacagaaga tatcgttaca cttgcagcct ctgcagctaa
    10561 tgatatttta tctgatgagg acaaagaaac tattgatatg gtgattgtcg ctacagaatc
    10621 aagtatcgat cagagtaagg cggcttctgt ctatgtacat caacttttag agattcaacc
    10681 tttcgcaagg tcattcgaga tgaaagaagc atgctatagc gcaactgctg ctcttgatta
    10741 cgctaaatta catgtagaaa aacatccaga ttctaaagta ttagtgatag cttcagatat
    10801 tgctaaatat ggcattaaat ctactggaga atccactcaa ggtgcaggca gtatcgctat
    10861 gcttattagt caaaacccat ccattctaga gctaaaagag gaccgtctag cgcaaacccg
    10921 tgatattatg gacttttggc gaccaaatta ttctgatgtt ccttatgtta atggcatgtt
    10981 ttcaacaaaa caatatctag atatgctaaa aactacttgg aaagaatatc aaaaacgttt
    11041 taatactagt ctaagtgact atgctgcttt ttgtttccat atcccttttc caaaattagc
    11101 tttaaagggc tttaataaaa ttctagataa taacctagat gaacaaaaga aagctgaact
    11161 acaagaaaat tttgagcact ctattactta tagtaagaaa attggaaatt gctatactgg
    11221 ctcattgtat ctaggacttc tatcgttact agaaaatagt caaaatttaa aagcaggtga
    11281 tcaaattgcc ttcttttcat acggtagcgg agctgttgct gaaattttta ccggtcagtt
    11341 agtagacggc taccaaaata aacttcagag cgatcgtatg gatcaactca ataaacgtca
    11401 aaaaattaca gtaactgagt atgagaaact cttctttgaa aaaacaatcc tagacgaaaa
    11461 tggaaatgcc aacttcaaca cctaccgtac tggcactttt tctctagact ctatttgcga
    11521 acatcaacgt atctacaaga aaattaataa ttaatatgac aaaaatttcc tggacaggat
    11581 tttcaaaaaa atcccctgaa gaacgcattc attaccttga agaacaagat tttttagctg
    11641 attcttccct agaaatagtg acgaatcagg acttactctc tttatcacta gccaatcaaa
    11701 tggctgaaaa tgtcatcggt agaatagcct taccgttttc acttgtccca gatgttttgg
    11761 ttaacggtaa agtctatcaa gtcccatatg tcactgaaga accgtcagtt gttgctgctg
    11821 caagttttgc tgctaaaatt ataaaacgat ctggtggttt tctgacaacc gtccataatc
    11881 gtaaaatgat tggacaagta gccctttatg atgtacaaga tagtcaacac gctaaagaat
    11941 ctatattaaa tcaaaaacag caattattag aaatagccaa cgctgctcat ccatcaatag
    12001 tcaagcgtgg aggcggtgct tgcgatctaa ctattgagat taaagaaggc tttttgattg
    12061 tttatcttat ggtcgataca aaagaagcca tgggtgctaa tatggttaac accatgatgg
    12121 aagcattatc ttcgccatta gaagacattt caaaaggcaa aagcttaatg tcaattttat
    12181 ctaactatgc tactgaatct ttagtgactg ctacatgccg agttgattta agatttctaa
    12241 gtcgccaaaa agaggaggcc atcaagttag ctcaaaaaat gacgatggca agtcaattag
    12301 ctcaagtaga cccttatcgt gctagcacac ataataaagg tatttttaat ggcattgatg
    12361 ctatcgtctt agctactgga aatgattggc gagctattga agctggagcg catacctatg
    12421 ctgtcaaaga tggacaatat cgaggattat ctaaatggtc ttataaggtt gatgataatt
    12481 ttctagaagg tactctgacg ctaccgatgc ctgttgcaac aaaaggtgga tccatcggta
    12541 ttaaccctag tgtccaacta gctcacgatc tactaggtcg tcctaatgct aaagagttgg
    12601 caagtattgt tctttcgatt ggactagctc aaaattttgc ggctcttaag gcattggtta
    12661 gcacaggaat tcaagctggt cacatgaaac ttcaagcaaa gtcgttggca ctacttgctg
    12721 gagctaaaga agaacaaatt tcagaagtcg ttaaacagct tctagactct aaacacatga
    12781 atctagagac agcacagaaa attgtcaata aacttactaa aagtaaaaaa agaatttgag
    12841 aaaaatcatc tcaaattctt tttttacttg acttgaattt ccataagtgt ataatctagt
    12901 tataaaaact agattatatg gagttttaaa atgaataaaa atcaataccc aaaatgggaa
    12961 gaattacctg aactagatct gtatcttgac caagtacttc tctatgtgaa tcaattaatt
    13021 aatcctaaaa caataactaa cgataaacta ctcaccgctt ctatgatcaa caattatgtc
    13081 aaacataatt atatatccaa acctattaaa aaaaaataca atcgccgtca agtagcacga
    13141 ttgattgtta ttacagcttt taaacaagtt tttgctattc aagaaatcag tcaaacatta
    13201 gaaattctga cagctgacaa tcattctgag gaagcatata acggatttgc tgcttgtatg
    13261 aacaaagaag aggttcacga tttaccacca gttgtcatta gtgcctgtca gacgttgaac
    13321 ctttatcaag aaacacaaaa attagtttta gaactaggag gaaaaaataa aaatgcttaa
    13381 agaaatgtct atcaaattaa gtcctcaact ttcctttggc gaagagcttg caaatagtat
    13441 cactcacgct gtaggagctt tgcttatgct aatcctatta ccaatcaccg ctgtctatag
    13501 tcacaaccac ttcggattac aagcagcact tggtacctca atatttgtca cttcattatt
    13561 tttgatgttt ttatcatcaa gtatttacca ctccatgact tatagcagtc tccaaaaata
    13621 tgttctacgc atgattgatc actctatgat ttatattgct attgcaggta gttatactcc
    13681 tgtcgcattg tcacttattg gcggctggtt aggatatctc atcatttttt tacaatgggg
    13741 gattacatta tttggtatcc tttataaaat cttcgctccc aaaatcaatg ataaatttag
    13801 ccttgtactt tacctcatta tgggatggct ggttatcttt attttccctg ctattatcac
    13861 aaaaactgga ccagcttttt ggggattact cttggctggt ggtatttgct atactatcgg
    13921 tgcactcttt tatgctcgca aacgtcctta tgatcacatg atttggcacc ttttcattct
    13981 attagcttct attttacaat acattggaat tgtctacttc atgctataaa atttaactac
    14041 attttattga ttattttttt gctaaaacat gatataataa taatcatcta aatcttagag
    14101 agaaggcgtg gacatatgaa aagtgcttat atctttttta atccaaaatc tggtaaagat
    14161 gaacaagcat tggcacaaga agtaaaatct tatttaatag aacatgattt tcaagacgat
    14221 tatgttcgta tcattacacc ttcatctgtt gaggaagctg tcgccttagc aaaaaaagca
    14281 tcagaagacc atatcgactt ggttattcct ttagggggag atggcactat taataaaatc
    14341 tgtggtggtg tctacgctgg tggtgcttat cctactatcg gactagtacc tgccgggacc
    14401 gtcaataact tttcaaaagc tttgaacatc ccacaagaga ggaatctcgc tcttgaaaat
    14461 cttctaaacg gtcatgtaaa gtccgtagat atctgtaaag ttaacgacga ttacatgatt
    14521 agcagtttaa cacttgggct ccttgctgat attgctgcta atgtcacttc tgaaatgaag
    14581 cgtaaattag gaccttttgc tttcgtaggt gatgcctacc gtatattaaa gcgtaatcga
    14641 tcatattcta ttactcttgc ttacgataat aatgtacggt ctttacgcac gcggttacta
    14701 cttattacca tgaccaattc gattgccggt atgccagcct tctctccaga agcaacaatt
    14761 gatgatggac tttttagagt ctacactatg gaacatattc acttcttcaa attattgttg
    14821 catctaagac aatttcgtaa gggagatttt agtcaagcaa aggagataaa acattttcat
    14881 accaataatc tgactatttc aacttttaaa cgtaaaaaat cagctatccc taaagttcgt
    14941 attgatggcg atccaggtga ccaattgcct gtaaaagttg aagtcatccc taaagcctta
    15001 aagtttatca ttcctaactc attaccataa tataaaagga cctagacata agtattagag
    15061 taaatactat ctgtctaggt tcttttttgt tatacttaaa gcaataaata tcattgaaag
    15121 gaagtatacg atgacaacat ttactgctaa atttattgac caagagtggg aagtcccagt
    15181 agaatcaggt cgctaccata tgattgttgg ggaattttgt ccttatgcac aacgtccgca
    15241 gattgcacgc caactgcttg gtttggataa acatatttct atcagttttg tggatgatgt
    15301 tccaagtgat ataggactta tttttagcca accagaacag gttactggtg ctaagtcgct
    15361 tcgtgatatt tatcatttga ctgacccgac ctacaaaggt ccttatacta tcccaatttt
    15421 aattgacaag actgataatc gtattgtctg taaggaatct gcagatatgc ttcgactttt
    15481 tactacagat ttctctgatt tgcatcaaga ggatgctcct gtccttttca gccaagaaac
    15541 tgcttcactt attgataatg atattaaaga cattaataat aacttccaaa gcttaatgta
    15601 taagttagct tttcttgata aacaaacaga ttatgatact tatagtaagg aattcttcac
    15661 ttttctagat caaaaagaac acttactagg acaacgtcct ttcttgctcg gagataatct
    15721 aagtgaggtt gatattcatt tctttacacc attagtccgc tgggatattg ctgggcgtga
    15781 tcttctacta cttaatcaga aagcgctaga agactaccca aatatttttt cttgggctaa
    15841 aactttatac aatgacttta atttaaaaac attaaccaat ccacaatcca taaaaaataa
    15901 ctattactta ggcaaatttg gaagagctgt tcgtcatcat accatcgtac caacaggtcc
    15961 aaatatggta aaatgggaaa aataaagcta aaaagctaag gcaattcaca gccttagctt
    16021 tttagctata ctagtccaaa gtggaccaat caaattcaat aaaagtcttg aagagatctc
    16081 cataagtacc actataagca atatttttat ctttataagt cattttcgta ataggataat
    16141 tatcaggaac agtaacacat cccattaaaa ctcgaataaa atcatttgga ttttcaaaaa
    16201 cttggcatcg ctcagtgtcg taagcatctt cataatacag tttaatcatc ttattacctc
    16261 tgacgtttta caaggaaact ttgacaggaa gctatatcta cttcacaaaa ataatatcat
    16321 ttagcattgg cttccttcaa ttgaccatac aatatataat tgacttgtcg taaatcagtt
    16381 atttttttac aattcagtgc acacattatc attcttaaat cttctttcca agaattgaga
    16441 attgcaataa catcatctac aggataacgc tcaactaatt ctaaaactgc tctagaaagt
    16501 ccaacagctt tagcacccaa cactaaacat tttaccatat ctaaaggatg acgaatgcca
    16561 ccagatgcta ggatatccat tttgtccatc atagattgag cgtttattaa agattgagct
    16621 gttgtttgcc cccaggtatt gagatacgaa cggtcgcgtc cacgttgatt ttcgatgtaa
    16681 gcaaaactag tccccccacg ccccgaaata tctacagttg tgatacctat atcgtaagca
    16741 tcctttattg attgtaaatc catcccaaaa ccaacctctt ttaagataag tgggcattgg
    16801 atattgtcga cgtattcttt taagtgagaa cgccacatgt gaaattcacg ctctccttca
    16861 ggcatcaaaa gttcttgcat cacattaaca tgaacttgta aaaaaatggg gttcatagcc
    16921 ttaacggttg actctgctgc tggtaaaggc ttgtctaacc ctatatttgt cgccagtttt
    16981 aaatcaggat acaagtctgt tgttgggtag gaatcatctt catcgttttt gagagctgcc
    17041 gaatatgaac ctgtcgccat aacaatccct gtagcttgag caacctgtgc caatttatga
    17101 ttgactgctt tccctttttc actacctcct gtcatagcat tgatataaaa aggaaattca
    17161 aagctttgac cagcaaaatg tgttgataaa tcgatatcgt tgacattata ttttggtaag
    17221 gatgaatgta ttaactcaat atcgtcaaaa ctattataag gcgactgata tttaagagca
    17281 tattttatgt gatcatcttt tcgatttgtc atgacaatgt ctccttacta agtagttcaa
    17341 taccagcact ctcccaacgc ttgattagag tttgattatc atttttatta aaggatatgg
    17401 ctataccaca atcgcctcct ccagatcctg aagattttgt gactatatct aggcctttgc
    17461 ttgcctcttt aagcttttga agtttatcgg tgtaaataga tggatgaaga ttaaataata
    17521 acaagctaat ttcttctaaa catctcttaa tcgcttcttt atctccttct ttaaaggctg
    17581 aaagtaactg gacaacttga taatttgtat catctaaaaa acgctgcgta atagttgatt
    17641 taactatatt tatcatttct tttgaaatgg acggttgcat tgtccatccc actaaaaaat
    17701 ccatctccaa tgaagcttgt attttggtaa tacgataacc ccattcagct tccaatactt
    17761 gttccaaggg ttttgtttca atcatttttg aaacagccct acgatcaaaa gctgaataag
    17821 aaattaaatg ctcataagca atacaagcaa tatctcccat tgagccatta tcacctcttt
    17881 ttaagagaac gtaggcagat aatttaaaaa gcaagtcgct tggcatctct atctcataaa
    17941 gcgcagccat agctttaata gtcaaaacga caacacttcc gctagagcct ataccaaatt
    18001 taagtccata gcgctctaat tttcccgtaa tttcgaggtg aatcggtttt atgtttttgc
    18061 caaaattaat caaccattct tccataagca atatcgtttc ctgaatgaga ctatattgct
    18121 tatcaggctg caagcttgcc gtatagttaa acatatcaga ataaaggctg tagttatctg
    18181 caaaagtagc taatgcagtc atataaatag gaatgttctt taaaatagct acttgtccag
    18241 gatacagtat cgcgtattca cctgcaatat ataatttgcc acctgtttgc accttaacca
    18301 tctgctaatt cctttgtcgt tgaaacaata atacgataat ctttctccaa gatagcagct
    18361 aatgcctcaa ggtcttgacg taagcaaagt actttaacat ttggacccgc atccatcgta
    18421 taataacatt catgaccttt ttcgcgaagt ttcttcacaa catccatagc tttatatgtc
    18481 tcttctgtca aatatgaaaa ggctggagtt gctgttttag ttgttgagtg cattgcaagt
    18541 gcattacgct ctgtcagttg acctaccttt tgaaaatcat tattttttag gtatactaac
    18601 atatcttgat agtcttgttc agattgcctt acccattcat taaaagtcgt tgaggtttcg
    18661 gtgcagattt tcataccttc acggcttgaa atcggctttc ttttgtcatt taagaccagc
    18721 ataatcatag ctaaatctaa atcagtgtga actttataga tatcaccact atctttatcc
    18781 caagcagcaa caggtccaaa aaaagagcgt gaagaagagc ctgaagcaaa cttggcttct
    18841 tgagctaact gtgattggct aaggttagta ccaaaaaaat cattgcaagc ctttactaag
    18901 gctgataaac cacttgatga cgatgataag cctgcagctg ttggcatatt attagttgtt
    18961 tcaatcttta caaagccgga cttattttgt cgaaagcgat ccaagatagc tgacattttg
    19021 tcatgttcat gatcattttg aagaacacca gagatgtaaa attcatcttt cttagcatcc
    19081 ttcccaaggg tagtcaaacg tgtttcagtg tacatgtttt caagtgttag agaaatacta
    19141 ctggtagctg gaatcatttt ttcagcatct gcttttcccc aatatttgat aatagcaatg
    19201 ttagcataag acttaacact tatacttttt ccgtccatgt gttgacagct ccttctcttt
    19261 ctaattgctg acttaagcgc tcagcttctc ttttttcttt tacaagtgcg ataatacaac
    19321 cacctaatcc gccgccactc attttagcgc ctagagcacc acaactaata gctacctcta
    19381 ccagatgatc agcttctaaa cttgagacac ccaattgttt taaactttga tgcgctttag
    19441 tcatcaagct accaagttgt ttggaatcac ttttatgaat agcatcctct gatgcctctg
    19501 ctaaataacc taattctttt aaaaagggta gtactgcctc gccagatgat ttcaccttat
    19561 ccactgcttc tttagtatgc ccatgaattc ctgtatcagc aataactaaa taagcattca
    19621 aatgcatggg tactgttgaa aaaccaatat tgcggataaa tttaatagta ttttcactaa
    19681 gacaagtttt tgcatctaat ccactcggat ttgaatgtgc tatcatttct gctcgattaa
    19741 caaggcattc taacaaatct gcctccaaat cttcatcaaa ataatcaaaa actgcacgaa
    19801 tagctgcaat agcaactgca gctgatgatc ccataccacg tctttctggg acttgtgact
    19861 caatatgata tgcaatttta gaagaagttt tacctaaata gtccaaggca gcaaaaatgg
    19921 ctgtcgacaa aggatcagtc atatctaatg ctattaattg aggagcctct tcaataaggc
    19981 aagttacttc aatatttttt aaaggaatag caatggctgg ataaccatat actactgagt
    20041 gctcacccat caagataatc ttgctatgag cctttcctat accaaacttt tctttcatct
    20101 ttttctcttt acaattctat taccttacta ttttaatata aatccttaaa aaaagcgact
    20161 ttaacgtgat aataacataa attaaaggaa ctacaattgc agtcccttta atcttaaatg
    20221 taaatgacaa aggtaatggt atcatgttta taattcacat cgatacttcc tttaaataga
    20281 tgcaccaaac tagttacgat agaaagacca ataccatatc ccgatttttt gttattatgt
    20341 gactcatcct ctcgataaaa tcgttcaaaa aacttgctat agtctatgtc tttaccattc
    20401 ttataagtat ttgaaacttc aagcttagct cgtctcaaac gacttgaacg tgataactta
    20461 actgtcacag tccccatagg atcacaatac ttattagcat tatcaaccaa taatgtaacc
    20521 aattcaaata atgatttttc ttcagctttg acatgaatac ctggttggat actcattata
    20581 aagtctttac catctttaat gattggacct ttaaaatctt ctgcagcatc ttttgtaata
    20641 tgtgaaaagt ctaagtcaac catacttata tcaggctgtt cttcaaagcg ggcaagactg
    20701 accatgccat taattagacc cgttaaacgt tggatttgat catttgtaga tttagtccac
    20761 tctgattcac cactcatcat ttccaccaat tcattattag cggagatgat agcaagtgga
    20821 gtttttagtt catgtcctgc attggtaata aaacgccttt gcttttcata gttagcaaca
    20881 aatggtaaga taactctccc tgataaaact gaaacaatta ccatgaagac gataaaactt
    20941 gccaaggaca tccatattga aagttgcaat aatctttgat tatcaagata ttggttggtt
    21001 gtatctaaaa ataccactaa ataatcattt ttaccaacct tttttagtag ataagaataa
    21061 gtactaaaat tataggtaat atgacctgaa tcactatttt gtgatacgat acgcttggca
    21121 taatttttta tttgcctatc gcttacttca aagatatttc tagaatcaaa actagtcacg
    21181 ttaccttttg catctgttcg cgcactgaaa taacggaatt ggaaaatagc gtccgttgat
    21241 aggtcatcac ctaattcttt actaaattca agtttattcg gtatacgtcc attgttgtct
    21301 gcaaggatag ttaacacacg ataaatttca ttcttcgttt gatagtgatt agcagagttg
    21361 aggacaccga ctactgaaaa taaaacaaca agaatagcta gcgcagctat tccgataaaa
    21421 cgaagtcgta aatttctaaa cattagccac catttatttc acttaattta tagctactac
    21481 cttcttgacc aataatctcc aagttagcac taattgcctg caatttttgt ctcaaataag
    21541 aaacatagat aaagacgtat ccctcatcta catcttcttg gtccttatct gctccccaga
    21601 catgttcgaa aagttgttgt gtagataatt ctttatcatg gttcaacatg aaaaatgcta
    21661 acattttagc ttctttaccc gctagacgaa tagtgttttc acattgcaac tcttgttcac
    21721 cgactgataa ggttactctt cccaatgaca atacattagg tgtgaaatct tccaatcttc
    21781 gggacataga gcgaagcctt gctaataatt cttttagaga gaagggtttt gttaaataat
    21841 catctgcacc agcatctaga cctgttactc tatcatcaat ttctgccatg gctgttagca
    21901 taataatgtg agatttattt cctgattgtc taatttcttt gacagcctca acacctgttt
    21961 taacaggcat catgacatcc ataaccatga catcatatgc attttgatta gctaaatcaa
    22021 tagcagtctg accatcgtag gcgacatcaa caacataacc ttggtgacta attgccgtac
    22081 taaggacacg tgacatctgt tcttcatctt ctgcaattaa tactctcatt ttattccttc
    22141 tttgttccac tctctatcaa ttgacgaatc gtttgcatcg ttaaatcaac agatgccatt
    22201 tcatcagcac aacgttttaa ttctcttaca attcgttcgg gattttcaat atcatgcttg
    22261 tatttcatct gatgttctaa actagcccaa gaatcttgcg caattgtacg caactgaatc
    22321 tcaatgtaat atttaccttc tgaattaccc aaacagtctg gatagggagt atcaatttct
    22381 aaaatcacat ggtaagagcg atacccattt ggcttaacat gctggatata gtctttttca
    22441 actacaatac gacaatcatc aaatgactta atacgttcta caatttgata aatatcgtta
    22501 acaaaaccac aaataatacg gactccaata ctatctctaa tagcttttag tgcagaatct
    22561 actgttacag gtaactgttt acgacgacat ttctctatca tactatctga tgtcttaata
    22621 cgaccaatga tatgttcgta caatttatag cctgtttcaa ccttagcttt atcattttct
    22681 aattgtatac gctggctgaa atcttctaaa attaatggta gatatcgacc ataatctcca
    22741 taaatatttg tttccataag aaaccttcct attctctctt aagctccatt atatcataga
    22801 aacttaaggc aaactaaaaa ggaatgagaa ttttttcttc ccatttcctt ctaaaatgtt
    22861 catgttaagc tgattcgaat tgagaattat acagatcagc ataaaaacca ctttgagaca
    22921 tcaaaacatc atgattgcct tgctcaatga tattcccatc tttcatgacc aaaatcaagt
    22981 ctgcgttttt aatcgttgat aaacgatgtg cgataacaaa tgatgttctt ccttccataa
    23041 gtttgtccat agctttttga attaactctt ctgtacgtgt atcaacagat gaagttgctt
    23101 catctaagat aagtaaggga gaatttttca aaagagctcg agcaatggtt aataattgct
    23161 tttgtccaac tgacaatgtc acagcgtcat caaggacagt atcataccct ttaggtagtg
    23221 tcattataaa atgatgtata ccgactgcct tagcagcttc ttgaacctca tcatcactga
    23281 ttgtcgtctg gttaaaaatc aaattttctt taatagttcc ctcaaacaac catgtatctt
    23341 gaagaaccat cgagaaagca tcatgaactt gactacgttt catgtcttgg atattgatgt
    23401 catctatgga aatactacct gaattaacat cataaaaacg catcaataaa ttaacaattg
    23461 tcgttttacc tgcgccagtt ggtcctacaa tagcaacttt ctgaccaggc tgtgcttccg
    23521 aagtaaagtc atgaataata gtcttgttat cactataacc aaacttgaca tgttcaaagg
    23581 acacatgccc cttaaactta ggaagattta acactttgtg tttatcctct tgcatatttg
    23641 tttcatttaa aaattcaaaa acacgtccca tggctgcact agcagactgt aattgtgtaa
    23701 ttccttgagc aatctgacca ataggctgtg taaaaatacg aacataggtc ataaaagcaa
    23761 caattgtacc catagtaatt gttcctttga tagctaacat ggcaccaacc acacacacca
    23821 tcacataacc aaaattacca ataaactgca tcagaggcat cataattcca gagaaaaatt
    23881 gtgatttcca catggaactg tgaagtttat catttaatag ttgaaaatct gacttagcct
    23941 gctgtcttgc attatgacta gtaacaatgt tgtgtcctga gtaaatttct tctacatatc
    24001 cagatatatt agccaaattc acctgttgct ttttaaataa aggctgactc ttcaccataa
    24061 tgattgaaga aagagcgaat cctgaaaata ccgatataat cgccgttgtt gctaaatgcc
    24121 aatctgtttt gaacatcata attaatgaac caaccaataa cactgtagat gcaaccatag
    24181 taactaaact ttggttaaaa gactgtgtca tcaaatccac atcatttgta acacgagata
    24241 aagtatcacc ttgagtatgg ctatcaaaat aactcaaagg tattttattg attttatcag
    24301 caatagcatt gcgcatgcct tgagcaaatt tttgaataat agtcgttaca ataaaacttg
    24361 ctagatagga tactaatgca cctaaaacat ataatactgc caattgccct gcaatatgac
    24421 cgattttggc taagtcaata ttaccagcca agccttttac cataagatcc gtcatctctt
    24481 taagacggtc tggtcctata actgttgtca agcttgataa agttgctaac acaattgcta
    24541 acgcaaaaag aagtttgtaa cctttgaggt acggagacaa attagagaaa aaagacgttt
    24601 tttgtgattt agttttcatt ttccaattcc tccttagaca attgagaata ggctatctct
    24661 tgatacactt cattatttga taacaattcc ttatgtgttc cttgcccaac aactttacct
    24721 tgatctagca ctaaaatgag atcagcatcc atgatagttg aaatgcgttg agcgacaatt
    24781 aatttcgtca tagcagctgt tttttctttc agctcatggc gtaaaatatg atctgtttta
    24841 taatctaacg ctgaaaatga gtcatcaaaa atgagaattt cagggtgacg tgctaaagca
    24901 cgtgcaattg ccaagcgttg tttttgtcct cctgaaaagt tactaccacc ttgagcgact
    24961 tctgtatcta atcctttttc tttagcctcg ataaattctt tagcttgcgc taattcgagt
    25021 gcttgccaaa tttcttcatc actaagttcc tccctgccac tctctcccaa aatcatatta
    25081 gaacgtactg tcccactaaa gagaacagct ttctgtggaa tatagccaat tttattattc
    25141 aaatcattgt gagagtattc ctttatatca atgccatcaa gtttaattgc cccctctgtg
    25201 gcgtcataaa aacgtggaac taaattgact aaagtggatt ttccagaacc tgtagaccca
    25261 ataaatgcca ctgtctgacc agtatctgct ttgaaagata catgttcaat aaccgcttca
    25321 gaatctttac tataatgaaa tgacacatct tgaaactcaa tttgaccttt ttgtgcactt
    25381 tgaatatgac tattttcttt aaaagttact gttggggcta aagttagtac ctcattaata
    25441 cgtctagccg atactaaagc acgaggcatg atgataaaaa tagctaccat catcataaat
    25501 cccataacaa cttgcatagc ataagatgaa aaaacaacca tgtttgaaaa aagatccact
    25561 cgatcagtga tagctgattt agcagccaat acagatacac ttgttcctgt aactttaggc
    25621 acagagatat cattaatcaa ataagagcca atccaataaa tagctaaagt caatccagat
    25681 gaaaccactg tcatcacagg attcataagc gacatcaatc tataaacaaa aaggttcaat
    25741 tttgttaaat cgtcattttc acgtttgaac ttatctgttt gatacttttc agcattgtag
    25801 gcacgaataa ctctaacacc agagagtgcc tctctagtag ttgcattaag cgcatctgta
    25861 aaaccttgga tttgacgttg tctcggaaaa gcgagtaaga gtagaacgct aagtaatatc
    25921 ataacgataa taaccgctac tccaactgcc ccagtccatt gatcgctttt accccaaatt
    25981 ttagtcaaag cccatattgc cataattggt ccacgggtaa caacctgcat tcccattaca
    26041 ataaggatct gtagctgagt caagtcgtta gttgtgcgcg ttaatagaga ggggatagaa
    26101 aatcgcttaa tttctgcatc agaataatcc atcacatggt taaaaatatc tgaacgtaat
    26161 cgactagtaa aactagctgc tgtcctagca cctaaaaaac ctaccattac tgctactaaa
    26221 aaactaccaa atgaaaaggc aagcattttt agtccaggat cccaaagagc ctctaacttt
    26281 gtacctttag tttgcaacat acgtgtgata tcagacatat attctggagt ctttaggtcc
    26341 atccaaaccg ctagacacac taatatagta ctcgccacaa tcatcaacca ttctttacca
    26401 ttgagtcgct tgaaaatatt aaacatcgca aactcctttt actttcatat ttatcctatt
    26461 atcctaaaat agttaatcaa aatcttccat cgccatattt ttagctaatg ttctaataac
    26521 ctttttggta gtacgtatct cttctttagt aatacctgct aacatggtac tgtgcaattt
    26581 ttctaagaaa atttcaaatt gtgtagcctt ttgtttacct agatgagtca aataaaggta
    26641 tttcacacgc ttatctgtct tagatggaac aatcgcaata aatccatttt tttccatccg
    26701 ttttaccaaa ttagaagcta cagacttaga gatgtgtaaa atttcttcag catcttttat
    26761 agacatatcc ttgtcaggat gtttatagag atgcatcact aagtgtcctt gtggacccgc
    26821 taaatgttca acatcatact cttttgcata acgatctaaa tacttttcaa gttgattaac
    26881 aagtattcgt gctttttgaa gaggattctc cataataata ctcctttaat agttatcgtg
    26941 agaactattt taaaacaacc tttattaatt gtcaattaaa agttaccgtg agaactattt
    27001 ttacattaat atttctaaaa aaaacctatc attcgtcaaa taaaaaatga tttcgcttac
    27061 tttttaatat taaaaatata tgttcgcttt ttatattttt aattataaat ttatattgat
    27121 ttttaatgta aatgatagta aaatacaaat gtaatattta tttaatttac aggagattta
    27181 aatgaaaaag aaaattattt tgaaaagtag tgtccttggt ttagtcgctg ggacttctat
    27241 tatgttctca agcgcattcg ctgaccaagt cggcgtccaa gttataggcg tcaatgactt
    27301 tcatggtgca cttgacaata ctggaacagc aaatatgcct gacggaaaag ttactaatgc
    27361 tggcactgct gctcaattag atgcttatat ggatgatgct caaaaagatt tcaaacaaac
    27421 taaccctaat ggtgaaagca ttagagttca agctggtgat atggttggag caagtccagc
    27481 taactcaggg cttcttcaag atgaaccaac cgttaaaaca tttaatgcaa tgaatgttga
    27541 gtatggcaca ttaggtaacc atgaatttga tgaaggtttg gcagaataca atcgtatcgt
    27601 tactggaaag gcccctgctc cagattctaa tataaataat attacgaaat catacccaca
    27661 cgaagctgca aaacaagaaa ttgtagtggc aaacgttatt gataaagtta acaaacaaat
    27721 cccttacaat tggaaacctt acgctattaa aaatattcct gtaaataaca aaagtgtgaa
    27781 cgttggcttt atcggaatcg ttaccaaaga catcccaaac cttgtcttac gtaaaaatta
    27841 tgaacaatat gaatttttag atgaagctga aacaatcgtt aaatacgcca aagaattaca
    27901 agctaaaaat gtcaaggcta ttgtagtcct tgctcatgta cctgcaataa gcaaggatga
    27961 tattgctgaa ggtgaagcag cagaaatgat gaaaaaagtc aatcaactct tccctgaaaa
    28021 tagcgtagat attgtctttg ctggacacaa tcatcaatat acaaatggtc ttgttggtaa
    28081 aactcgcatt gtacaagcgc tctctcaagg aaaagcctat gctgacgtac gtggtgtcct
    28141 agatactgat acacaagatt tcattgaaac cccttcagct aaagtagttg cagttgctcc
    28201 tggtaaaaaa acaggtagtg ccgatattca agccattgtt gaccaagcta atactatcgt
    28261 taaacaagta acagaagcta aaattggtac tgccgaggta agtggcatga ttacgcgttc
    28321 tgttgatcaa gataatgtta gtccagtagg cagcctcatc acagaggctc aactagcaat
    28381 tgctcgaaaa agctggccag atatcgattt tgccatgaca aataatggtg gcattcgtgc
    28441 tgacttactc atcaaaccag atggaacaat cacctgggga gctgcacaag cagttcaacc
    28501 ttttggtaat atcttacaag tcgtcgaaat tactggtaga gatctttata aagcactcaa
    28561 cgaacaatac gaccaaaaac aaaatttctt ccttcaaata gctggtctgc gatacactta
    28621 cacagataat aaagagggcg gagaagaaac accatttaaa gttgtaaaag cttataaatc
    28681 aaatggtgaa gaaatcaatc ctgatgcaaa atacaaatta gttatcaatg actttttatt
    28741 cggtggtggt gatggctttg caagcttcag aaatgccaaa cttctaggag ccattaatcc
    28801 cgatacagag gtatttatgg cctatatcac tgatttagaa aaagctggta aaaaagtgag
    28861 cgttccaaat aataaaccta aaatctatgt cactatgaag atggttaatg aaactattac
    28921 acaaaatgat ggtacacata gcattattaa gaaactttat ttagatcgac aaggaaatat
    28981 tgtagcacaa gagattgtat cagacacttt aaaccaaaca aaatcaaaat ctacaaaaat
    29041 caaccctgta actacaattc acaaaaaaca attacaccaa tttacagcta ttaaccctat
    29101 gagaaattat ggcaaaccat caaactccac tactgtaaaa tcaaaacaat taccaaaaac
    29161 aaactctgaa tatggacaat cattccttat gtctgtcttt ggtgttggac ttataggaat
    29221 tgctttaaat acaaagaaaa aacatatgaa ataataaaaa taggtttgga tattttatcc
    29281 aaacctattt ttatatcaat atgccttcca aatgatctaa ctcgtgttga caaatttgag
    29341 ctggaaaatc tgtcaacttg atttgctgtt ctttccaatt gatatcacga taagaaatcg
    29401 taatgtgacg atatcgttgt gtacttctgc aacctatgag agaaaggcag gattcttctg
    29461 tctcatatgg atccgatttt gcaactaata cagggttaaa cattactaaa tttgtaatac
    29521 ccacattgat gataatgaca cgcttcagac taccaatcat atttgctgcc atacctacac
    29581 aataattttg attagcatgc aaagtatcct gtaaatcttt tgctaagctc acatctgctc
    29641 tactagccat ttgtgatttt tgttgtaaaa agaaagtatc tctaacaatt ggttttatca
    29701 tttattttaa cctaactcta ttatgataat actagtatac cataggttag ctaaggacta
    29761 tgataagaga ttaaacaata ccttgtgcag acatagcttg agcaactttt aaaaagcctg
    29821 caatgttagc acctagaacc aggttaccac tatcgccaaa ttcatcagtc gcttcggaag
    29881 cattcacaaa aatagttttc ataatacgtt gcaattcttg atcaacttct tcaaaagtcc
    29941 atgccctacg actagaattt tgtgccattt caagcgctga aacagctact cctcctgcat
    30001 tggcagcttt agcaggacca aaagcaaccc ctgccttttg gaaaacttta atagcaccca
    30061 aggtagatgg catatttgct ccttctgtta ctgccaagac accattttca actaataact
    30121 tagcgtcttc ttcgtctaat tcgttttgag tagcacacgg gaaagctaaa tctgctttaa
    30181 tagaccagat actaccttga tctgctggtg tgaaattagc ttttggatgt ttctctgtat
    30241 atttgacaat acgtgctctt tcaacctctt ttatctgttt caaagtttca aggtcaatac
    30301 cgtctggatc ataaacataa cctgaagaat ctgacacagc tacaacagtt gcacccaact
    30361 cttgtaattt ttcagtagca taaatagcca cgttaccaga tcccgaaacc agtgcaactt
    30421 tgccagttaa atcttgacca cgcgctgcta gcatttcttt agcaaaatag actgccccat
    30481 aaccagttgc ttctgtacga gccaaagagc caccataagt cagtcctttt ccggttaaaa
    30541 ccccattttg ataaccattt aaacgcttat attgaccata aagataacca atttcacgtc
    30601 cacctactcc aatatcgcca gctggcacat ctaaatctgg tccaatgtat ttttgaagtt
    30661 cggtcataaa actttgtgtg aaacgcatca cttcattatc tgatttccct tttggatcaa
    30721 agttcgaccc tcctttgcca ccaccaattg gtagacctgt cagtgaattc ttaaaaattt
    30781 gttcaaatcc taaaaatttg acaatagatt gtgttactgt agggtgaaaa cgtaaaccac
    30841 ctttataagg accaatagct gatgaaaatt gtacacgata tcctcggtta acttgaacct
    30901 ggcctttatc atctacccat ggaactctaa aagaaatcac gcgctcaggc tcaaccaaac
    30961 gttctaaaag attttcttca atgtactgag ggtatttatc aaaaactggt actagtgact
    31021 caaaaacctc ttcaaccgct tgtagaaatt cttcctcatg ctcattttgc ttcttcactt
    31081 tctctaaaac acttgctaca tatgtctttc cgcttgtcat ttattttcct ccgtattgtt
    31141 ttaatatagt tagcattata aattgtcaga attttcttgt caactatttg ctatggtatc
    31201 attattagga gcaattttaa aggtaattat ttgactttta ctgactttcg ttctataatg
    31261 agacaaatat ttgattggag tatctatatg aaatggttcc cacttgttgg aggtataatt
    31321 gccttattag ctggcttcta tctctttata aatcctgtaa cggttgttgc aactctgggt
    31381 tggttcattg ctttatttat ctttatttat ggcatttcaa atttagtacg ctacttgcaa
    31441 gctgattctt atcaccgtag cccttggtat ctggttcaga gtatccttgc tattattttt
    31501 ggtatcattt tactatccag ctcagcaatg tcactttcaa atactgtcat gactatttta
    31561 gcatactggc tactggtatc tggtatttta cgacttttgg gtggtttcca aatgcaaaaa
    31621 attaatttcc caggtgccaa atcctataaa cgttcggctt atattgcact cattttaggt
    31681 attattttac tgattcaacc taccttatca gcagccatca ttggaagatt tgtagccttt
    31741 atattaatag caattggagc ttcggctatt tacactttta ctcgtattaa ttattaaaag
    31801 gacatgtttc gtgtcctttt tttatctatt gatgacgtca tttactattt gaaaattggc
    31861 tattatataa ttcgtaataa aatcctttat ctgctaggag tttctggtga ttaccttgtt
    31921 cgatgatttg accatccttt agaactaaaa tgttgtctgc ctcttgaatc gttgatagac
    31981 ggtgtgcaat gacaaaacta gttcttcctt ccatcaattt tttcatagct ttttgaataa
    32041 gcaattccaa ccttgtatca acagatgaag tcgcttcatc taaaattaaa attttaggat
    32101 ctgctaataa agcacgtgca atagtcaaca gttgcttttg tcctaaggag atattgcttg
    32161 attcttgatt catcaccata ttgtagccac caggtaacgt tcgaataaag tggtcaacat
    32221 tcgcagcttt ggcagcagca actatatcct catcactggc ttcaagatta ccgaatctaa
    32281 gattttcttt tattgtacct tcatataacc aagcatcttg caacaccatg ccaaattgtt
    32341 gccggaaatc ttggcgtgat aggtgtctaa tatcgtgacc atctacggtg atagcacctt
    32401 cactaacatc gtagaatctc atgagtaagt ttataagtgt tgtcttacct gcacctgttg
    32461 gccctacaat tgcaatcatt tgccctggtt ctacgtttaa gttaaagtcg cggataagtg
    32521 gcttatccgg agaatagtga aaatcgacac catgaaagct gacttgaccg gttaagtcat
    32581 ggctcaattt ttcagtgact tgattggctt cctcttcttc atctaagact tcaaaaatac
    32641 gttctaagga agattttgca ctttgcaaaa cacctgctaa ttgtgtaatg gtttgaacgg
    32701 gttgactaat ttgccaaaca tactgcacaa atgcctgcat gttaccaatt gttaaggtac
    32761 cagcaataac ttgtaaaccg ccaacaaatg caatgataag gtaaataaaa tcagagatac
    32821 cattcaatac tggcatcata attcctgaaa tgaagctagc tttgaaacct acatgacgta
    32881 gattatccgt tatatcacga aattcttgac tagacgcctc ttctctacca tataatttta
    32941 tgatattgaa accagttaat ttctcttgta caaaaccatt cagttctccc agaatctttg
    33001 cctgttcttt aaagtaaggt tgggatcttt ttagtatcgc ttgtgcacta aaataagtta
    33061 caggaataca agcaatgata atcatggcta atggtacatt tagataaagt accataacga
    33121 ctactaaaat aatacttaaa aaagcattga taatctgtaa aaagctctgt tgtaaagcat
    33181 ttgagaccgt ttcaacatca cttgtaaaac gtcccaacat atctccaaat tggtgcttat
    33241 caaaatacga tacaggaact ttattgattt tatgattcaa atcatgacgg atatcacgga
    33301 tacttttttg aacgacagta gtcataaaat aatttgagcc ataagaacct aattcataga
    33361 aaacacctct gaaaaaataa ataatcaaaa taccagcaat ataaggaacg ttcaattctg
    33421 ccccagaaac tcccttagcc atatcaacaa ggttagcagt caactctgtt atcgctaacc
    33481 ctaaaataaa aggctccaga acgctcataa aactagataa aactttcaaa aaaatcgcta
    33541 agaaaagagt agctttgtag cgagttaggt aagaccataa tctcaaaaaa acagattttt
    33601 ttctcataca atcactctcc ttctgataag ttttggttct tcagttgtga ctcagcgatt
    33661 tcacgataaa tagcattatt ttctattaat tgagcgtggg taccacgacc gacaatttcg
    33721 ccttcatcaa ggacaataat ctgatcagca tccataatcg tacccaccct ttgagcaact
    33781 atcaaaactg taaaatcacc ggttacttct tttagacgcg ctcttaaagt agcgtctgtc
    33841 ttataatcga gagcagaaaa tgaatcatca aaaatatata aatctggatc tttaacaaca
    33901 gccctagcaa tagataaccg ttgtttttga cccccagaaa gattgctccc accttcagct
    33961 aaatgcgttt caaaggcttc ttggtgactc tcaataaact ctttagcttg agaaatatca
    34021 accgcttgtc taagatcatc aatagtagca tcagcttttc catattttaa attctctcct
    34081 attgtccctg taaataaaag agctttttgg gggataaatc caatcttttg gcgaagtgat
    34141 ttaaggttat aatctcttac atcaactcca tctactaaga tttttccaag tgtcacatca
    34201 taaaaacgtg gaatcaaatt aacaagagaa gattttcctg aacctgttga accaataaaa
    34261 gcaattgttt ctccaggctt agctttaaaa gaaatatcat gcaaaacggg actctctgtt
    34321 tctcctggat aagcgaatgt tacattatca aattctaaat gccctttaag tttcgtatcc
    34381 gtaacacctt cggcattagg attgatagag attggcatat ccatcacctc tctaatacga
    34441 tggcttgata ccaccatacg aggatacata gtaaaaagat tggcaaatag caaaaatgaa
    34501 aagagagcat ggaagctata ttcgataaaa gcaactaaat cccctatttt aatagcacct
    34561 ctttgtaagg gatccaaagc aaaccaaacg atagccacaa tcattgcaat aataatttga
    34621 acgaaaagtg gttctgttag ccctgttaat ttaaaaagac cagttgaagt atctgtgtaa
    34681 cgttggttag cgacttgaaa tttttgtgat tgaaaattct ctcttgcaaa ggctctaaca
    34741 acgcgtaacc ctgttaaatt ttcacgaaca tattgattga ttttatcaag catagtctgt
    34801 tgtctttcag ataaaggttt tgtttttata gctacatata aaacgactcc taccaaaaga
    34861 ggcatcgcaa ccgctacaag ccaagccaaa gatggactcg taattagtat cataaccacg
    34921 ctaaaaatca ttaccatagg agttactagg cctaaacgta aagacatttc agcaaattgc
    34981 atcaaaacaa aagtatcgct tgtcatacgt gtcactagtg aagatacacc tatctgttca
    35041 tattcatgat gggagtattc ttgaagctta gcatacatat cattacgcat atctctaatc
    35101 attgttgtcg ttaagcgact agatgcgtaa gccatcgtaa tacgcccaat aatacctagt
    35161 acaacaaata taaacatgat gaacgtccac agataaactc cagtccgatc accttttgta
    35221 acgccattat caatcatacc cgctaaggct gtgggcattc ctaggataac cgtcacaaaa
    35281 agcatagctc ctagtaaatc aagccataac caattggggt accttttcaa ataagaccaa
    35341 atatatgcca tatatgtctc cttttctttc ctcttatctc aaaataatta atccatgttc
    35401 actaatccac tttcaactta taaatcttta atcatccaaa tatccatagc tgtatgacct
    35461 tgatcatttg ctattggttg cgataaggca caatatccca tatgcttata aaccgcagtt
    35521 gccctactca aagtactggc tgtctctaaa taaagttgtc tatacccaat ttttcgagct
    35581 tctacttcaa tcattttcac taaatcagta gcaagcccct taccacggaa acgttctgca
    35641 atgtacacct tctgcatctc tgcaattaga tttttcagcg gaccaaagcc gccacagcca
    35701 ataatctcat ctctctcctc aatgacaaag aatcctgact tctctatttt ttcgtagtat
    35761 gaggtcaaat gatctaagtg agggtctgaa tatgctgtat caggtttatc taaatcatag
    35821 gcgcgtaaac tttgtcgaat taattgtgca actgcttggt catcattttt aataatcgga
    35881 cggataatca tgtgtctaac tcactaaggt actcataacg gtcatacttt tctagaagtg
    35941 cttcattttt tgcatctaat tccttttgta aatcagataa ccttgtaaaa tcactaccac
    36001 atgtttgcat atcattttct atacgagtga tagtattttc caatatcata atatcgtctt
    36061 caattgtcgc ccattcttgt ttttcaaagt aagacattct tttacgactt tgcttttcac
    36121 gacttgtctt ggtactctct tttttactga taacttcatt attttgctca ttaaatgctt
    36181 tttcatctaa ataatcagta taattaccaa aaaattcacg gatatcgtta tcttcaaacg
    36241 caataatttt attagccact ttatctaaaa agtaacgatc gtgactaact gtaatcacag
    36301 gaccaccaaa gccttgtaaa aaattttcaa gaacagttaa tgtagcaata tctaagtcat
    36361 ttgtcggctc atcaagtagt aacacgttag gcttttcaat caggattttt aaaaggtaaa
    36421 gtcgtttttt ctcaccacct gataattttg caatttgtgt tccatgtgtc gaacgtggaa
    36481 agagaaattg ctccaatagt tctgtcacac ttgttgtacc gacactagtt ttaacctcat
    36541 ctgcaacctc ttgcaaataa ttaataacac gttttgagcc atctatatta ttaagttgtt
    36601 gtgaaaagta acctacacgt atcgtttcac caatagagac attacccgaa tccggatgta
    36661 aatcttgaac aattaaatta agtaaggttg actttccaac accattatct ccaacgatgc
    36721 caatacggtc tttattttga attaacaaat taaagtcttt caagatagat ttatctgggt
    36781 aagaaaaaga gacattttca aaattaataa cctttttccc aattcgactt gtttcaaatg
    36841 tcatttccaa atcgcttgta tcgcttgttt ggtgtaaatc gttttttaga ttttgaaaac
    36901 gattaatacg agcctgttgt ttcgttgcac gagcttgtgg ctgagtacgc atccaagcta
    36961 gttcctgttt ataaagctgt ttctttttat gtaaactagc agcatcacgc tcgtcttgtt
    37021 ctgcacgaag tcggacataa tcctgataat tgccttgata ttctgtaatc tgtgccttat
    37081 ctaattcaaa aatacgtgtt gcaacattgt ctagaaaata acgatcatga gttataaaaa
    37141 gcactgtctt tttactattt ttcaaaaaat tcgttaacca tgcaatagtg tcaatatcta
    37201 agtggttagt aggttcgtct aagagcaata aatctgcatc atttaataat acttgcgcta
    37261 attgaacacg tcttcgtaat cctcctgata attcaccaac cgacaactgc aaatcagtaa
    37321 tacctaattt ggataatact gttttgactt cgctctcaat agaccaagca tctaaagaat
    37381 ccatttctgc cattactttc tctagacgtg attgcttact ttcttcgtag tgattaagca
    37441 ataattcata ttctttaatt aaagccatct ctcttaagtc agaagaaagt acggtgtcca
    37501 aaattgtctg agaatcatca aagtctggtt cttgttttaa ataagcaatc ttataatcat
    37561 tagctgatga aaaaggggaa cgatcaccat caaaacctaa ttcacccgaa ataacatcta
    37621 atagtgttgt ctttccagtt ccattgacac caataatccc aatacggtct aaactatgga
    37681 tgataaatga aacattacta aagaccgtct tatcaccaac cgacttagtc aatccatcta
    37741 ctaaaaaatc gctcatataa tcctctctaa tccatcaagt actgcttaac aaaatgtaaa
    37801 atagcctctt cttcattaat cagttgtcct aaaacaatgg ctaattcaat ttgagagata
    37861 atatctccca tctgtggacc aggttttatt cctaatttct tgattaaggt gccaccattg
    37921 acaactatct catgcctatc atggattgct aactcactat ctagttgttc tattttttcg
    37981 aaatcaacca tcaagcctct tgctttgcga ataccttccg cttgttttat tatagttttt
    38041 ccataacgat aaagatgttc tttagtcaat cccatttgtt cacgaatacg ataaacatcc
    38101 actatatgct caacatcttt ttgaaagtga gttgatgtct tccatttttt taaaaaagtc
    38161 tttgtgtgtt caacttccat actcaagatg agtgatgccc aagcttgttc tgatgtctca
    38221 aataaaaaat ctttatctaa ctgggacaaa aaggcgctta attgtaattc cctatttttt
    38281 aagcatggta gataattaaa agcatgacta tctattagtg ccagcattcc tttacgccaa
    38341 taaggtgcta atagaagttt atcaaattca ataaaagaac gctcaacaga tattttctcc
    38401 aataatgacg cgtgcttttt cattgcctca aaagttgttg tttcgatatc aaaatttaga
    38461 ctagcagaaa aacgcaaacc tcgcataatt cgaagagcat cttcattaaa acgctcactg
    38521 gcgagtccta ctgctctaag aaggtgatta tctaaatcat ccaatccatg aaagagatca
    38581 ataacttctc catcttcatt taaagcaaag gcattaactg tgaaatctcg acgttttaga
    38641 tcttcttcta gcgacctaac aaaattgacc tgactcggac gcctataatc aacataaacc
    38701 tcctcagtcc taaaagtggt aatctcatat tcaccacctt tttccaatac taagactgtt
    38761 ccatgttcaa taccaacatc aactgtcctt ttaaaaatct gctttgtttc ttctggataa
    38821 gatgacgttg ctatatcaac atcatgaatt ggacggtcaa gtaaaacatc ccgaacacta
    38881 ccacctacaa agtatgcttc ataaccggct ttttttatct tttttaatat tggtaaagcc
    38941 ttctgaaact cagaaggcaa ataatttaat ctcatcatac tattccaaat ctaaacgaac
    39001 taaagtcgca ttattatttc ttataaatgg attcagaaac aaaaagaact gcctaaggga
    39061 cactccctac ctactcaaat tatcaaaaaa gaatctccta acttcttctt tgatatctgc
    39121 aataaggatc aacctccaag caactctttt tatcacacat caattttttg gggtaatacc
    39181 aaaggcaacc gcaccttcac caagatgagt tgcaataacg ccaccaaatg atacaatttc
    39241 taaatcgtct ttaagaccag ctttagctaa caaattatat aattgttctg ccttatcttg
    39301 tgctctagag tgaataatag caatatcata ttcaccatct gctgtcattt ctttgacaat
    39361 ctcagccaag cgttttaagg cttttttctc tgtacgcact ttctcataga caacgatttt
    39421 accttcttca ttaaaatgta agaccggctt gatacttaaa aggttaccaa tgatagctga
    39481 accgtttgac aatctaccac cttttactag atgattcaaa tcattaacaa caataaatcc
    39541 ttcaattttt tcaatttgtg attgtatctt gttaactatg acatcaaaat ccatcccttc
    39601 acgtgaacac atcaaagcat ttctaactaa attgccctgt ggtgctgaag ttattttagt
    39661 atccggaaaa gcaattgtca aatttggatg ttcttcaata agaaactgaa tattctgcca
    39721 gaaacctgat atccccgctg caatgaataa accaaggacg tgtgtatacc cttctttctc
    39781 caattgacat aagagatcat ctagttcagc taagctaggc tgacttgttt taggtaattc
    39841 ttttgatgcc gctagcttat cataatattg gtctaatgtc agattttgtc cctcaatata
    39901 agttttacca tcaatgataa ttggaatatc taaaacatag acatcttcat gattttcgat
    39961 agcctcaggc aagtaagctg aggtatccgt aataagtgct aatttcatta actattaaac
    40021 tccaatgtta ctcctggtct atctaaagca atttctgtaa cttcataagt cagacgttta
    40081 acgatttcta aaagaggagc tgctagattc tcaacttcat cttgactaaa ctcactaggc
    40141 tcattgataa ggcgcccttg gatatgagcc atttgtgaaa tgacaccaga aataacaaac
    40201 tcatcacgta caataacaaa ttgtaacact gctacaatgc tcgttacttt tgctgcttca
    40261 tctctattag ctaattggaa agtaacttca aaatttgttt ttggggtacc attttcctct
    40321 tcccattcta aattgcgcgc atcatagtgg tattgattga caaattcttg ttcacgaatg
    40381 acttccatgt attttctcct tgatagacaa tatgtattat tatagcataa atttgtagat
    40441 tcttaaagca ggtgagaatc tagaacttct tcttaggatg tctatcaatc acttcttgat
    40501 tgttactcaa ggctgctaaa cctttttctg taagtgccaa ccctctgaca acaaacaact
    40561 tccctaattc ctcttctgta aaagcaatag ctattgcctg atctaaatca gagcgtttca
    40621 tctttgacaa gccttttacg tttttttctt ttaagaatag ttttaaaagt ggagcagata
    40681 agtgatctaa agaatcaagc ggtgactcca ttcttacaaa tccatttttt attaaaaatt
    40741 ctaggtgttc ttttccattt ataccataaa tgtattcaaa atattttgaa aaacttgttt
    40801 cagttgtgaa agttccaaac tgtatacgcc ataataagat aatatctcca ggcaagagtc
    40861 cttcactagt ttcttccata tttttcctag gaacaacttt actaaaatta atttcagcca
    40921 agtctctttc aggtgagata taggggatat taggataatt tttgtaaatt tcttcaatag
    40981 ttttcatagt catattgtag caaaaaagcg cttataaaaa caagcgctaa ggcatggggg
    41041 aagtcacctt tttgaaaaat tcaatttcat tttctgatat tctaaccatg ttaaaattat
    41101 catgataaca tcaaatacac atagaaaaat cattaaaatg ctatgactag aaaaatattc
    41161 ccttagttgg aaaatgataa acccaacgaa aactaaaatt gataaagggt aagcaaataa
    41221 tttctcttta tgtaataaaa taagtgctac taatttgata ccgccatgaa ttaatagata
    41281 gatagcggct aaatgactag ccgaatcatt aatataactg cttattttca agaaatgagc
    41341 agaaataaaa ccatgaatcc caccgtgaac ttcaccttca gttaaaagaa ataggagatg
    41401 ttttatttgt gtaggtttaa aataatataa taaaacgcca gctactactt gtccgactgc
    41461 aaatagcgtt tttagataca acataattga aaaacttgta tcaactagtt ttgtttcttt
    41521 ttcattccat ttaatcattg cttacctcct ttcctcaatc atattatcca tttttttgct
    41581 taaataaatc atatcctatt gaaaaaagat tagcaattat taatgattaa tctaccattc
    41641 ttttacaaca taaaaaggcg aatagaccca ataacgatag tttattcacc ttttattcaa
    41701 tgtaaatttg gataagttga gattaactat tttgttttac ggaacaagcc aaagagtaca
    41761 cctgagacta ccgctccaat tagaataaat agaatataaa gtaaaggatt acttgttaat
    41821 gcgatgacaa aaatccctcc atgaggagcc atcaacttga taccagctaa accaacaagt
    41881 gctccagtta aggctgaacc tacaataaag ctagggattg cacgagctgg gtcagcagca
    41941 ccaaacggaa tagcaccttc agtgataaaa gataacccca taacgatatt tgttagacca
    42001 gattgacgtt cttcattatt aaatttatct ttaaataata aagttgccac aaatactgct
    42061 aacggtggaa ccattcctcc agccattaca gctgccataa caacagagcc gccatttgca
    42121 acagttgctg caagagtacc tgtaccaaaa acataagctg ctttattaac aggcccaccc
    42181 atatctacag ccatcatacc accaactagt aagcccatca agactgctga actaccagat
    42241 aaaccttgta agaaagtgtt aagagctgtg ttgatagctg ccattggaat gtttacgagc
    42301 aacatcaaga aacctgtaat caaaacacct aacaatgggt atagaagaat tgatttaata
    42361 ccttcaagcg attttgggag acctgaaagt aattttctaa gtagtaggat aactccacca
    42421 gctaagaaac cacctactaa agcccctaag aaacctgatg gcacacctgc taatgctaag
    42481 gttgctttcc caccttctgc aaaaggtact ttaccgaatg ccagtccact tgatgcaatt
    42541 gaaccagcta cgaagccagc tactagtcct ggtttttcag ctattgaata agcgatataa
    42601 cctgccaata ctggtaacat aaatgcaaat gctgcgccac cgatattttt gaataaggct
    42661 gcaatttcat ggtatgaacc taaattactt aattggtctt ttggtacacc aagtatgtta
    42721 tcaaaaagga aagcaatcgc aatcataata ccaccaccaa taacgaatgg taacatttgt
    42781 gatacaccac ccataaggtg tttataaaaa gcagagccta agcctgattt tccatcagat
    42841 tcacctgatt gtttatcatt ttgatttttt gcatgatagg tttgtgcctt attatcgaga
    42901 ataatgttaa ttaagtcttc acttttttta ataccatcag ctaccggacg tgataccaaa
    42961 ggtttgccat caaaacgatc catctctaca gccttatctg ctgcaataat aacaccctta
    43021 gcccttgcaa tatcacttga agtcaactta tttcctacac cagaagcgcc atttgtttca
    43081 actttaatac ctacacccat ttcagctgct tttttcttta aagcttcttc tgccatatac
    43141 gtgtgggcaa ttcccgtagt gcaagctgtt actgcaacaa tataatctga acctttactt
    43201 gttgattgta ctgtttgtgg agctactgtt tcttgagaaa tgctattact atcaaaagta
    43261 gcaataatgt catctggcgt ttttgcttga cgtaattgat cagcgaaccc ttcttttaaa
    43321 agatatttag atagttctgc caaggcagct aaatgcgtat cattagcacc atctggcgca
    43381 gcaatcataa agaataaatc agttggctgt ccatcaagtg cttcgtaatc aacacctgat
    43441 gcagattttg caaaaaggac agtagcttct ttaacggcag catttttact atgtggcata
    43501 gcgataccat cacctaatcc agttgatgtt tgtgcctcac gcttcatgat accatcttta
    43561 aaaatagcaa agtttgtcac aacacctgtg tcaactaatt ttgtaatcat ttcatcaatt
    43621 gcagcctctt tactggttgc ttttaaatcc ataatcataa cttctttttt tagtaggtct
    43681 tggattttca taatttttct acctcaactt tattataaat atcttggata aactcagctg
    43741 ttgctaaatc atctgagaaa gtggttgctg taccacaagc tacgccccat tttagtgctt
    43801 ctactggatt ttttgactta acaaattctc ctgtaaagcc agctaccatt gaatcaccag
    43861 ccccaacgga atttttcact tcacccttaa tcggttttgc aaaatatgaa gcttctggag
    43921 ttactaacaa tgcaccatcc cctgccatag aaacgataac attcttagct cctttatcta
    43981 gaatcttatg tgcatatttt tcgatatcag gcagaccttc tagctctacg ccaaaaatat
    44041 ctgctaattc atggttattc ggttttacta aaagaggctg ataagcaaga gcgtctaaaa
    44101 gtgtctgacc ttcaaaatcg caaacaactt ccgctcctgt ctttttagca attggaatca
    44161 atgtattata gaccttattt ccaaggctag ctggtgctga acctgcgaaa actactgtgt
    44221 cctcaggtgt tagtctcgaa agaagtttct ctagacgatg aagttgttca tttgttatcc
    44281 taggaccccc gccattgatt tcagtttcaa tttttgcttt tactttaacg ttgatacgtg
    44341 tatcttcatt aacagaaaca aagtcagttt tgataccttc tgatacaagt ccatcttcaa
    44401 caaaatgtcc tgtaaatcca ccgataaaac ctgtagcagt attgtcaatt tttaagcgtt
    44461 ttaatatgcg actaacatta attcctttac cacctacata tttatcatcg ctagtcatac
    44521 ggttaacact acccaacaat agtgtatcta gacgaacaat aaaatcaatc gatggattta
    44581 atgttactgt ataaatcata cttgaatcac cttcattttc tcttttatta ttgtcataag
    44641 ttctgaatca gtcttattag ttactaaatt aatgtcattt atctctttca ctttagcaaa
    44701 tgtaacttgt ccaattttag atgaatccat tagaataaag gtttgttgac tattattaat
    44761 aatagcttct ttaatagcag cttcttctaa atctggtgta gttaaataaa cttcatctat
    44821 accattcatc cccagaaaag ctttgtcaac cgttatctgc ctaatctgat taatggctac
    44881 ctggccaata ctagcatctg ttgaatgttt aaccgctcct ccgataatga ttgtttttat
    44941 ccctctatca actaactttg cagcatgatg aatagaattc gtcaccaccg ttaattgctc
    45001 gtgagggaga aaatcaatta ataattctgt cgtagttccg gcatcaataa atataacatc
    45061 ttgctttgct atcagctttg cagtttgtct agcaatatcc aatttttttt gaacgttttt
    45121 gatagctttt tcttgattac ttaattcctg gccaagtgag tatggtaatt cagcaccacc
    45181 atgcaccctt tttaaaaaac cttcagattc aagttcatct aaatccctgc gaactgtaga
    45241 ttcagaagtc tctaagatag acgttaattc atctaaagta acactcttat tttgctcaag
    45301 tcgtgataag ataatttctt ttctctttga ctttagcata gtccctcctt gaattgaaat
    45361 cgtttgcaat ttaattatat ctctttttct ttcaatgtca agcattttct tccaaaaact
    45421 atcaataaac taaaaaagcc taatctaaag attagctttt acaaatactt taatttctct
    45481 tcaaaacctt tttaagaagc tgaattcctt tgtatttaaa aggacttgga taatagttaa
    45541 aagttcccat tttacgaatg atataaccat taaaattctg tttaaagcga agaatactat
    45601 ctgaaccatc aaaagttccc tgaataccta ggaggttata gaaagtagaa cctcttttga
    45661 gagcctcttg cataacatat tcctgaagaa ctgctggtgc ataaaattta ttaaattctg
    45721 tgtaggatcc tgaaaagaaa taaactgtct ccttaagact ataaacaaat agactaccag
    45781 ctaaaacaac gtcttggtta tcatactttt gaataaatga ctgaacttct tgtcgtcgtg
    45841 tttcaaaact agccagttga ttaattattt cagagcgttg cctatgatac ttaggagagt
    45901 ctgcattttc cctaaaacgt tcatcaagca atttaagctt attttccaat gcttcagctt
    45961 ttatttgcaa gttatggtcg tattctctaa aatttaaagt ggcaatcaca aattcagcct
    46021 tgccttcaaa gctatcgtaa aaatcttgat aataatctaa ggacttatcc atataatcac
    46081 gtctatttga cgtagaagtt gttatctctt taaataaatg tagctcatca cgtttaagaa
    46141 cgcgaacctt gattccaaaa gacattgctt tcttaaccaa agctcgtcct gtcttagaga
    46201 atgaagatag taacgtttca gaagtgacac cttctaaatt tttaacatag tgccaagata
    46261 aatatttacc agtaaaacca gttgttaaac catcatggtg ataacctgaa ctggtaaaat
    46321 cttcaatcag attatcattt ccctgattac taggaactcc cgaactagtg aataattggt
    46381 aatcatcata aggctcaact attaattcta gaacaccgtt ggatttggca tagccttgca
    46441 atgctttata aaattgtttt agatacttag aatttgaatg aactggtcct gaatcaattt
    46501 tcatttgaaa accaccagtg actggcataa tataagataa actgattatc tctagtttat
    46561 tttctacttg atatcccaaa tacctaacat catagccgcg tttttctaaa agtttagcca
    46621 tctcaggtgt ttgtaaaaat gattgtgaat cataatttcc tgaatgttct ttaaattctt
    46681 ctattgttaa ttctcgaagc gtcataaata cctttcttaa ctatttccta cttttctcca
    46741 cattctatct attcgatact atccaaataa tataattttg acgtaaatct ccactgtttt
    46801 ttccaacaca cataatatta gtaaagctaa atctaaaaag atttagcttt aaatttcaat
    46861 atagcgacga ataccggaat aactcttgta tgaaatccac tgatgaccat ctactactaa
    46921 cacttgatca taatgtattt tttcaccctt ttgaaaatta aattccactg gacttgatac
    46981 tttaggttga cttttcacat ctacagtttt agtaaatgta taggtacctg ttttaggtaa
    47041 gttgggataa ctagtcggtt tagtcgcctc atctttcgct ttttcactac ttgtagttag
    47101 ctttttcaca ggaatatagc gacgaacacc actataagat ttgtaagaaa tccactggta
    47161 accatctgct gtcaatactt gatcataatt tattttgtca cctttttcta aagtaaattg
    47221 ggtctgactt gatattttag cttcattttt tacttcagta cttccgataa tattataaac
    47281 accagtcttt gctaaaccat tttcaatagg ttcttgagaa gaattagttc cagctactgt
    47341 cactttagtt cctgttacac ctacaagtgt cccactagct tcttggtagt ataaatgaat
    47401 attataaaga cccttctcat tcttatggtc agcaaatgat acagctactt tgtagttgcc
    47461 atccccagta gttacagctg tataccattt aatatcatct tgccctcctt gttcagtcca
    47521 aaccggtacc ttaacagcag cgataccgtt atcatcttta atattcgtaa ttaaaatatc
    47581 aaaacctgta gttgtttcgt tagaaatagt cagtctacca gttttagtaa tacgggcttg
    47641 tggttgagga gacacttttt ctttatcttc agttttttct actgaagatg ctttacctag
    47701 caaaacaaaa cgacgaacac cattgaatga tttataagat aaccactgat ttccttcaat
    47761 agttagtatt tggtcgtaaa aaattctgtc tcctttgtcc aatgtaaatt gagttggact
    47821 cgctacctta gcttcatttt ttacttctac tttatgtgaa aatgtataat ttccttgcgt
    47881 tgctattttc tcttgattat tgcttcctga atttgttaca ggagtaggtg ctttagtctc
    47941 tgaacctcct gatggatcta gtgactcaat agctgcgtat cgacgtacgc cacaaaaaga
    48001 cttatatgaa atccatttca cattatcttt attaaatact tggtcataga aaactttatc
    48061 acctttcttt gcatagaaag ctactggggc tgattttgaa ggtgtatttt tcacctcggt
    48121 ttctttgcta taaacataat tcccttgttc aggtaatgtt ttttccacca ttggctttgt
    48181 ctctactaaa ggagtagtaa tgtcctttgt tgccgtattt tgactattta caactttatc
    48241 agacgcttga acactatcat tattggaagt aatcacactt gacgcacttg tctcattgga
    48301 agaattatta gcatccacac tagtgcctgt ctgattatta acttgaaccg atgtagtttg
    48361 atccgcaaaa acaggacttg tgaccattat gaatgaccct aaaattactg atgctaaacc
    48421 aaatttatat ttacgtagag agtaagattg cttagtatca tttacttgtc cttttttcat
    48481 cataaacacc tacttcctta tttatttaca ctactattat aatatccttt ttgtaacatg
    48541 tcaacatatt ttagaaatat ttgtaataca aatgtaatat tttatgctgt aaatacttct
    48601 cgaaaaaaat acccaactct tattattctt agtataacaa aatcattatc attaagcata
    48661 tcaaaaaacc gggcactatt ctcccggttg attattattt tatgctaagt acattttctt
    48721 tagtgtgaat taattgtgtg attaatcgac agtaaggtgt atcaatgcct aggttttcac
    48781 cttttttatt cactgcgcca tttaagaaat caatttcagt taagcgctga ttttgaacta
    48841 agtcttgatg cattgaaggg taatgatggg ctgctttaac agatgtatcc ataacgtact
    48901 ttgttatttc ttcttcatca agctctacac cttctgcttt tccaacagta acaaattcat
    48961 ggataatttc atgtaccata tttacgccgt cttcactagc aaagagttga ccaattgtac
    49021 aatctagcaa ggcgcaagtt gaattcatcg ttccgttaac acaagctttt ctccaaatat
    49081 ttggcaaaac attttcgtca taagtagcct gtaattttgc ttcattaagc aattcagtaa
    49141 cgcgatgacc tgcttcttga ttgttaggat cgatactttg aagatttaca gaaccaactc
    49201 cttccagatg agcatgccct ggtcctttca accctgcagt ccaaactgtt actcccatca
    49261 aaatattgtg ttcagggata tattgacgaa tgacatcttc atgtcctaat ccatttaaaa
    49321 gacataagac ctttgttttc ttaccaataa tttttttaat atcttgaagc atatttggca
    49381 actgcattgc tttagtaaac aaaataatta aatctgcttc ttctgtcgca tcagttggct
    49441 tcatgatagg aagtttaaca aggtcctctg tatcacctgt taccttcaag ccattttctt
    49501 taatagccat aatatggtct gcccaattat caagtaaaat aacatcatga tttgttttag
    49561 aaatttgata tccaaaacga catcccatag cgcctgaacc agcaatataa actaacatac
    49621 aacctccaaa ttaattttga ccttcaaaaa cgaaaatatc gtctttataa agtttcagaa
    49681 tgtctttaca aattttgtgt gatacaaaac ctactgcaaa aggaataacg agccaagata
    49741 agataacaat caacatactt gctcctgctt caaatgaagc aatcggacct actgcaccaa
    49801 caagcccaaa accagacgaa gctggtgtcc ccaccaattt aaacaatggg actgttagag
    49861 aacttactgt agcagtcata agcattggaa tcgccattac tggatgcttt aggaaatttg
    49921 gcatcatcat cttcattgca cctaaagcaa tagcaattgg aactccagac ttattagcct
    49981 tcatcgttgc ccaaactagc actgctgttg ttgaagcaac tcccattgaa gctgcacttg
    50041 ctgacatacc attaagacca atcgctagac caatcgctac tgttgaaata ggacttacaa
    50101 taagcattga aaaagccatc gcaattagaa ttgacatcgc aattggttgt aaagtagtaa
    50161 aagaattaat tccttgacca ataagtgttg tcacatatga aacgtaaggg agtaatttcc
    50221 atcctaaata gcctacgcct gtacctatga taataggtaa caagataatg gttagggaac
    50281 caaatttatt accaaaccac ttaactgcta aaactgctaa agctgctgtt agcatcatat
    50341 tgatgagatc accaatccca cgcaattgga aactaccagt agcaactcct ttttgaataa
    50401 cttctgtgta agcccacgca ccagaaccaa tatatgcagc acctccaaca gcaagctgtt
    50461 gcattggggt gaatttaaac tgttgaccaa tcaaaaatcc tgccataatt ggtgtgaaaa
    50521 attggaatac ctgcacgatg tgtaagaact ctgcagcaag tccgtaagat aaaaatggtt
    50581 tcaaaaaagt ggctaggata gcatttggga tgagtgctac aacgattgca gttgctgtcc
    50641 cacctaacac tttattgata aaagaaccag ccgtctcttt tggtgttacc gtatttgtca
    50701 taaaatacct cgcttcattt atttaatatc attcataacg acaaagttaa ttatatcaca
    50761 aaataaatag gaagctttgt tattttttta acaatatata taatcctttt ttataaactt
    50821 atcagtttta tttatagtta ttttatcaat gatgttgaat ggactacacg gttatctcga
    50881 tcaggataaa tataattgat agcttggttt atagcgactg gtgcttcccc atatcctgtt
    50941 gcaatcaatt ctacctttcc tggataatta gcagcatctc caatagcata cacgccttct
    51001 tgagtcgtct caaaaagtga agaaacattg atactagacc gtttataatc caaattccaa
    51061 taacgtaaat ttttattaga agtagaaaag ccaaaactaa caatcaagtt gtctaaaggt
    51121 aattcaatta cttcatcacc tttaactttt tgaacgacca agctgctaac tctctgacta
    51181 tctccattta aacaaattgg gacataaggg gttaaaattc taacacctga tgcctttagt
    51241 atatccacac tatgctcatg cgctctaaaa gcatctctac gatgaacaat cgctacacta
    51301 gctgcaatct tatctaattc attggcccaa tcaacagctg agtcgcctcc accacaaata
    51361 acaacatgtt tacctgcaaa ctgttctaac tttgtaacgt tatagaataa attattatct
    51421 gcataatttt cttcattttc caaacctagt aaacgcggag caaaagcacc gttgccacaa
    51481 gcaaaaacaa tagcacgtga caaatgttga tctttatctg tttgaattgt gaaaacatca
    51541 tctatttttt caaatgataa aacttcttct tttaaacaga tttcaataga atctttaaat
    51601 ctctctaatt gttcaatatg tttatcaatt aattcacgtc cagtaatcac cggataacca
    51661 ggaatatcat agattttttt ctcaggataa aggatagctg gctggcctcc taattccgaa
    51721 aggctttcaa taattttaac ggatactccg cgtagtccag cataaaaagc agcaaagaga
    51781 cctactggtc cgcctcctac aatagtgata tcataaattg ttttgttcat ttactgtatc
    51841 tgttctttct atttctgttt taattttctc taagataagt ctctcttcat ccgtcattgc
    51901 ataattttct aacaagtctg gtcgacgttc tagtgttttt cgcaaacttt gctctaaacg
    51961 ccattttcgg atattttcat gatgaccact catgagaaca tcaggaactg tcatgcctaa
    52021 ataatcatat gggcgcgtat actgaggata ttctaataga cccgatgaaa atgaatcatc
    52081 ctgatgactt gtttctttac ctataacttc tggaatcaga cgaacggtag catcaaccat
    52141 tgtcatagcc gccaattctc ctcccgtaag aacaaaatct cccaaagata cttcgtctgt
    52201 tactaatgat tttatacgtt catcataacc ttcatagtga ccacaaataa aaattaattc
    52261 atcttctttg gatagttctt ctgcaaaatt ctgatcaaat gttctgcccg ctgggtctag
    52321 caaaataaca cgtgcttttt gagcatcaat tttaccaatt gtatcaaaaa taggttgagc
    52381 acgtaaaagc atcccttgcc ccccaccgta aggctcatca tcaacatgtc gagacttctc
    52441 agcattttct ctgaaattgt gataattaat ttcaagaagt cctctttctt ttgccttacc
    52501 aactatagaa tgttccagag gggcaaacat ttcaggaaat aaagttaaaa tatcaatctt
    52561 catcatctaa accttccatc aattcaactt gaacacgctg attagaaagg tccacttcta
    52621 atactactgg cgggatatag ggtaataaaa gatcacgttt tccatgacgt tccacaaccc
    52681 aaacgtcatt tgctcctggt tgtaaaattt ctttgatttt tccaatcagc tcttcccctt
    52741 catagacatc taagccaata atttcatgat aatagaattc accatcttta aggtcgctca
    52801 gttgattctc tgccaccttc agtgtgaaac ctttatattt ttcaatatca ttaatatgat
    52861 acattccttt gaattttata atgtcaaaat tcttctgctt acgatgacta gcaatttcaa
    52921 tatccataac aaactgattc ttttcatcga ataaagctaa ctcttgacct tttttaaagc
    52981 gttcttctac aaagtcagtc acgcttaata cacgcatttc gccttgtaat ccttgtgtat
    53041 tgacaatttt tccaacgtta aagtattcca tagtaactcc gatatttttg aaaataaaag
    53101 aaaaaccagc cgctaaaatg caactggcca cgatagtccg tacgggattc gaacccgtgt
    53161 taccgccgtg aaaaggcggt gtcttaaccc cttgaccaac ggaccatgtg aaactggggt
    53221 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
    53281 ccccaaaaga caaaatctag tatatcgaaa ttctagatta ctgtcaatat cttttcgaat
    53341 taaattttaa atttcgattt tatccctttt aataaagctc atatagagtc tatcaaagta
    53401 ttttatgttt catttttgaa atatattcaa aaactttttg gtcccttttt tcgataatag
    53461 cttgtaaaag tagttcatgg tactgcttat tattcatccc aatttctaat atatcttgta
    53521 tagccacacc tgagtcattt tttaaagaag atatatagcg tctctcagca ataatgatat
    53581 ctgctttttt tagtaatttt tcatctatct gagtcgtttg atagtttaca aaaaagaatt
    53641 ccgctatctt agatgtaaaa tattggttta acattacttg ataggtttgt aatctaatat
    53701 tactagatga aattacatat acagaaagag gagctaagaa agtctctatt aagttagata
    53761 attgcattgt tagtagcact gtctcatgtt ttgaaaaagt taaattccct cccttcaggt
    53821 taaaaatatc agccagacct ctatctaata ggtgatataa atccttagaa tagctcagcc
    53881 ttgatgtatt taaaaaataa aaatcaggaa taaaataata acaataacaa ataaaatgtc
    53941 tcaaaaaata ggcattaata tctttaaaat gctgcgagtc aataaattct ctacccaata
    54001 atcgaccaca aaaattctta aaggcttttg actttgatat cttataagaa gcaaaaataa
    54061 attcttcgtt gacattgata tcatctcgat tgctaaatag agggccatca ctaatacaaa
    54121 acactaaata tagataatca agtaaacctt cattcaactc tataccaaga tgtatagcaa
    54181 tacttttttg agctgctttg gataactcca tgtagagaat cgattttttt aattgattaa
    54241 aggattctgt ttgaggaatt tctactttat attcttttct tttccaagct aaattcacca
    54301 aaatataaaa atagtaatat tcttcagata agtctttaag gttgtagtca caccctttaa
    54361 aattcgagta ttcaataaaa tcccttatca ttacctcact tgtagcatca aaaggataaa
    54421 taataaaacc aaaatgatat tgtaacaaag caatcagcag cctaatctga tattctggac
    54481 ctgtcacttt atttttttgt attgataact taacactctc caaataaagt ttacagtttt
    54541 tctttattcg gtaggcactt gcaacggata tgaatttctt agccgtgaat tcagaaaaac
    54601 tgacattttg aagagcaata aaatatgcca aaaactgtaa catattagac tctcgataaa
    54661 tggtatgcaa agtctctagc ttagaaagtg tttcgtaatg gaacaatacc attgttctac
    54721 ttttgacaac aaggtagaaa tcagaataaa gtatttgtag ttccgaaata agaccttgta
    54781 aaatcttttc actcaaagca tattctgttt taaagtgatc aaggttaatt tctccatcat
    54841 caagtaaggc ggtcatcagg tatgctttca gttttatttt ttcttcgaga aaatcaaata
    54901 gcaatatctt atcctcctta ataaaaaatc agaaaattcc ctttcacatc cattatattc
    54961 taaaagacta aaaaactcac aatttttgtg tagatataac aaaaaagaca ggaagttcct
    55021 atcttttaga ataaattatt ctttctcatc aatgatgagt cttacttttt tcccttgtgt
    55081 tggtaccgaa tagacaatcg accttatcgc tgtaattgta cgaccctttt tgccgataac
    55141 acgaccgatg tcttgggtat ctaaatcaag atggtactct aaaaattctg ggccatcctg
    55201 aatttttatg gttaactgat ctggttgtga aatcaaaggt ttcacaatag caataatgag
    55261 attttcaatg gtatccatag gcttttatta ttttgagaat ttttgatcgt ggaatttagt
    55321 cataactcca gcttttgaaa gaaggttacg aactgtatct gaaggttgtg caccttttga
    55381 taaccattca agaacacgtt cttctttgat tgtaacttgg ttttctgcaa ctaatgggtt
    55441 gtatgttcca acagtttcaa taaaacgtcc gtcacgtggt gcacgtgaat ctgcaacatt
    55501 gatacggtag aaaggtttct tcttagaacc catacgagtt aaacggattt ttactgccat
    55561 ttttaaatag tctcttttct ttttctttat atttggtcgt tgatagctta actaacaact
    55621 ttaccttact aagtataaca taaatacaaa ctctgtcaag aaaaaatctt gacagagcat
    55681 atgttttttt attcatatcg taaagcatca attgggttta atttgctagc tttattagca
    55741 ggtaataaac caaatataat tccaataaat gctgagaaaa ttaaactgcc tatagccacg
    55801 ttaagtgaca cagaaggagt aatcttatct tgcgcattag caattaacat cgtacctcca
    55861 taggctagta ataacccaat taatccaccc aaaatcgtta agaccattga ttcaattaaa
    55921 aattgagcta aaattttacg tcgagttgcc ccaagggctt tacgaaggcc aatttctctc
    55981 gtgcgctcag ttacagagac taacataata ttcatcactc cgataccacc aactaggaga
    56041 gaaattgctg caatcgctcc aataacacct gtcataatac caacttgggt attgatagat
    56101 ttaactatag atgtcatgtc aaaattatca tagtacccat ccttagcatg actaatgtct
    56161 gttaaacgtt tgcctatttc tttaccaatg cgattggatt gcgatacatc attaagatga
    56221 aaatacatgt tttctgcttc tttagcacca aactccgaag ctaactgtgt attggttaaa
    56281 atagcattcc catcagaatt acttccatat aagcctgaac cagcttctgg atccgaataa
    56341 acccccacta ctaagtaaga tttatttttt acagtcacca ccttatttaa cgctgcttca
    56401 tttgtctgga ataatctctg agctaatttt tcttctagta aaataactct cgagaattgg
    56461 ttataatcct cctcttggaa ttgacgacca cttttaattt tgaacttttt gataccaagg
    56521 taagtgcggt taattcctgt tatattaacc gtctttgcct ttttttcaag gtaagctact
    56581 ggagcatttg tttgattggt tacatagtag ccactaacac catctacttc cttagcaatc
    56641 ttttttaacc atgattcttg aataattggt tccttacggt tggtatcact accttgcatg
    56701 aaagcccctt gagctccaaa gttatcctca tttttttggt cttcttttgt tttataataa
    56761 atctgaaggt tcttctgaga ctttgtaatc tcatttgtaa ccgaatcttt catgccctta
    56821 cctaaggcca taattaacac aactgatgca accccaatga tgatccctaa catggtcaga
    56881 aaagcacgca tcttgtgccc tagaatcgat gatagggcaa acttccaatt ttccataatc
    56941 gaatctcccc ttaatcaatt ctaatactat cagtagtatc tgctgtaatt tcgccatcac
    57001 gaataacaat tttacgagtc gcataatcgg cgatttctgg ttcatgagtt accatgataa
    57061 tggttttacc ttcttgattt aattccgtta ggaactgcat aatttgctca cccgtctttg
    57121 tatccaaggc accagtcggc tcatctgcta gaataataga tgggctattc accaaagcac
    57181 gcgcgattgc tacacgttgc ttctgtcccc cagataattc tgttggcaga tggttcatac
    57241 gctctcttaa ttcaacttta tctaaaaatt gttttgctaa atttttacgc tttttaggag
    57301 gtacacccgc ataaattaag ggtaattcaa cattttgtaa agccgttagt ttagataaca
    57361 agaaaaattg ttgaaagaca aaaccgattt ccttatttcg aacttgagct aattttgttt
    57421 gacttagttc ttcgacacgt ttaccattta aactataatc tcctgaagtt ggagaatcca
    57481 acaagccaat aatattcatt agtgtggatt tccctgaacc agatggcccc atgattgcaa
    57541 gaaattcacc ttcataaacc gtcaaatcaa tgttctttaa gacttgaagt ttttgatccc
    57601 cattttgata acttttaaca atctgatgca atctaatcag ttcttttcga ttagttgtca
    57661 cgattatttc acctctgatt tcttattaga gttaagatcg attgattcaa tattatcaat
    57721 tttttgccca tccttgaagg ttttacttgg attagtaacc acgatttgtc ctgctttcaa
    57781 acctgataaa atttcttgtg tcttagcatc agctttacca attttgactt caactttgga
    57841 aattttacga ttagaatcat tgtataccca aacaaagtgt ttattatctt tgtttatcac
    57901 agaacttgta gggacaataa ggtgcttatc tccattaact acttcaactg atacggtaaa
    57961 accttgtttt aatgcatcga gagggctagt aatatctact ttatatttat aatttacagc
    58021 actagagccg ttattagagt cattgttgtt tgcttctgct tctggataat ttgagatata
    58081 tgaaatttta ccttcccatt ccttgtcagg atagacctta gattttattt taacagcctg
    58141 gtctttttta acattagcca aatcatactc actcatcgtt ccttgtactt ggagtttacc
    58201 ttcagttgct acatggacaa gtacttgact agtttttgaa gctggatcaa tatcactatt
    58261 aacttcaaca actgtccctg atacgtcact tgtaataaca gtatcattca atgctttttg
    58321 tgctttattt acttctgcct gtgcatctgc ataagcatca ttcaaatctt gaagttgttg
    58381 gttgtatgaa gcattagctt gactttgata attttgctgt agacgattcg tcgcaccact
    58441 agtcgattga gtcccttgtc cttgtgatga tgaagaagat tgatcactta attccatagc
    58501 tggaagactt cctgttgtct ttagattatt aatctgacgc gctactttat ttaattgacg
    58561 attagcagtg tcgtaggctg cttgtgcagt tgttgtatca tattgaacta actgctgacc
    58621 agctgtgatt ttatcaccca ctttaactgt gacagttgct cgattacctt tattagcatc
    58681 aaaatacaca tactgttctt gattagcctt agcttttcct gtcaaaagag ttgaggacga
    58741 aacacttcct tctctaacat taaaaacttt gtagttagtt tttactgcac tcttattagg
    58801 ttgagattgt acccataaaa agccacctac tgctacaatt agtgccactg agagccctga
    58861 tataattgct ccttttttac taattcctaa attttgtcgt ttactcatgt ccatctcctg
    58921 tttgtattat taatatag
//
LOCUS       DATRSD010000002        58186 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRSD010000002 DATRSD010000000
VERSION     DATRSD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58186
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.002"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(131..685)
                     /gene="raiA"
                     /locus_tag="VBD85_000059"
     CDS             complement(131..685)
                     /gene="raiA"
                     /locus_tag="VBD85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO7132639.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEAQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
     gene            complement(762..1427)
                     /locus_tag="VBD85_000060"
     CDS             complement(762..1427)
                     /locus_tag="VBD85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO7132640.1"
                     /translation="MTCLLCHEIDLSQLTFVELMLLKPKQNVICQTCKGSFEALSREM
                     GCQTCCKQIPQKQCQDCIYWGKKGIEVNHFSLYRYNEAMKKYFSLFKFQGDYLLKDVF
                     TKEIKAALKKYKGYTIVPVPLSHEGYQNRQFNQVIAFLQSANIPYKNILSKKDGGKQS
                     ANNKEERLKQVQQFTLKNEAELGDNLLIVDDIYTTGATIAQIRKLLEEKGIKNIKSFS
                     LAR"
     gene            complement(1427..2716)
                     /locus_tag="VBD85_000061"
     CDS             complement(1427..2716)
                     /locus_tag="VBD85_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7132641.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGELTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELYKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYQG
                     VKQALKENGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEILRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            2772..3416
                     /locus_tag="VBD85_000062"
     CDS             2772..3416
                     /locus_tag="VBD85_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO7132642.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGAGGLIRAYSGSVANTIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            3507..4433
                     /gene="cysK"
                     /locus_tag="VBD85_000063"
     CDS             3507..4433
                     /gene="cysK"
                     /locus_tag="VBD85_000063"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO7132643.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSDDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(4585..5673)
                     /locus_tag="VBD85_000064"
     CDS             complement(4585..5673)
                     /locus_tag="VBD85_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO7132644.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNNDLIIGLGGGKTIDSAKAIA
                     DKVNLPVVIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDAKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLTVDRYVSNHQ"
     gene            complement(5741..6409)
                     /locus_tag="VBD85_000065"
     CDS             complement(5741..6409)
                     /locus_tag="VBD85_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7132645.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGREASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            complement(6419..8875)
                     /locus_tag="VBD85_000066"
     CDS             complement(6419..8875)
                     /locus_tag="VBD85_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO7132646.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQNLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANAKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKSDLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNDGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            complement(9056..10357)
                     /locus_tag="VBD85_000067"
     CDS             complement(9056..10357)
                     /locus_tag="VBD85_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7132647.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSSVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            complement(10359..10679)
                     /locus_tag="VBD85_000068"
     CDS             complement(10359..10679)
                     /locus_tag="VBD85_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7132648.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            complement(10696..11019)
                     /locus_tag="VBD85_000069"
     CDS             complement(10696..11019)
                     /locus_tag="VBD85_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO7132649.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            complement(11223..12206)
                     /locus_tag="VBD85_000070"
     CDS             complement(11223..12206)
                     /locus_tag="VBD85_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO7132650.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDPVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTIYQKS"
     gene            complement(12325..13080)
                     /locus_tag="VBD85_000071"
     CDS             complement(12325..13080)
                     /locus_tag="VBD85_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7132651.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSEIANLFQEAEITIQSLLQVP"
     gene            13201..13977
                     /locus_tag="VBD85_000072"
     CDS             13201..13977
                     /locus_tag="VBD85_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO7132652.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYQLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            complement(14024..14380)
                     /locus_tag="VBD85_000073"
     CDS             complement(14024..14380)
                     /locus_tag="VBD85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO7132653.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(14382..15782)
                     /locus_tag="VBD85_000074"
     CDS             complement(14382..15782)
                     /locus_tag="VBD85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO7132654.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLAEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVDQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            complement(15824..16465)
                     /locus_tag="VBD85_000075"
     CDS             complement(15824..16465)
                     /locus_tag="VBD85_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7132655.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVSRGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            complement(16458..17477)
                     /locus_tag="VBD85_000076"
     CDS             complement(16458..17477)
                     /locus_tag="VBD85_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7132656.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFRMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            complement(17474..18169)
                     /locus_tag="VBD85_000077"
     CDS             complement(17474..18169)
                     /locus_tag="VBD85_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7132657.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            complement(18330..20285)
                     /gene="pknB"
                     /locus_tag="VBD85_000078"
     CDS             complement(18330..20285)
                     /gene="pknB"
                     /locus_tag="VBD85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO7132658.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQTVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTLIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            complement(20285..21022)
                     /locus_tag="VBD85_000079"
     CDS             complement(20285..21022)
                     /locus_tag="VBD85_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO7132659.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            complement(21060..22382)
                     /gene="rsmB"
                     /locus_tag="VBD85_000080"
     CDS             complement(21060..22382)
                     /gene="rsmB"
                     /locus_tag="VBD85_000080"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO7132660.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFLEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            complement(22372..23307)
                     /locus_tag="VBD85_000081"
     CDS             complement(22372..23307)
                     /locus_tag="VBD85_000081"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO7132661.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            complement(23354..25744)
                     /locus_tag="VBD85_000082"
     CDS             complement(23354..25744)
                     /locus_tag="VBD85_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO7132662.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGVRVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAEVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDQIGK
                     EQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKQ
                     VAIMHSGLSEGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYYKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTSYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRDCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM"
     gene            complement(25818..26132)
                     /locus_tag="VBD85_000083"
     CDS             complement(25818..26132)
                     /locus_tag="VBD85_000083"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO7132663.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            complement(26155..26784)
                     /gene="gmk"
                     /locus_tag="VBD85_000084"
     CDS             complement(26155..26784)
                     /gene="gmk"
                     /locus_tag="VBD85_000084"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO7132664.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            complement(26951..27655)
                     /locus_tag="VBD85_000085"
     CDS             complement(26951..27655)
                     /locus_tag="VBD85_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO7132665.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPATMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYAKKGIEAPSYIKLFIQIGERAPEDF
                     TNSSSGIGGVKRLYKK"
     gene            complement(27705..28295)
                     /locus_tag="VBD85_000086"
     CDS             complement(27705..28295)
                     /locus_tag="VBD85_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7132666.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGGEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSEQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            complement(28297..29382)
                     /locus_tag="VBD85_000087"
     CDS             complement(28297..29382)
                     /locus_tag="VBD85_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7132667.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSVIIRGESKYHMIAWSTMLTYIFYMTIFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATVVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVVLKEV"
     gene            complement(29379..30119)
                     /locus_tag="VBD85_000088"
     CDS             complement(29379..30119)
                     /locus_tag="VBD85_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7132668.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQALSKLTPTYHLTQVILSPFANSFA
                     GFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            complement(30106..31002)
                     /locus_tag="VBD85_000089"
     CDS             complement(30106..31002)
                     /locus_tag="VBD85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132669.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGSLTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDTHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELIATLKKEGVTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAEAVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            complement(31131..32738)
                     /locus_tag="VBD85_000090"
     CDS             complement(31131..32738)
                     /locus_tag="VBD85_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO7132670.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            32927..33409
                     /locus_tag="VBD85_000091"
     CDS             32927..33409
                     /locus_tag="VBD85_000091"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO7132671.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQRINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            complement(33634..35097)
                     /locus_tag="VBD85_000092"
     CDS             complement(33634..35097)
                     /locus_tag="VBD85_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132672.1"
                     /translation="MSEDQKHPFFEPKKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDTEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVAIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKSYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQASSNKVSDTQITEQPNVTNGQSSSSAATINNQAAGTASGN
                     LERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYILEPVNIING
                     NGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(35110..36264)
                     /locus_tag="VBD85_000093"
     CDS             complement(35110..36264)
                     /locus_tag="VBD85_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO7132673.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            complement(36347..36715)
                     /gene="rnpB"
                     /locus_tag="VBD85_000094"
     ncRNA           complement(36347..36715)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBD85_000094"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(36734..37066)
                     /gene="gpsB"
                     /locus_tag="VBD85_000095"
     CDS             complement(36734..37066)
                     /gene="gpsB"
                     /locus_tag="VBD85_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO7132674.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            complement(37187..37705)
                     /locus_tag="VBD85_000096"
     CDS             complement(37187..37705)
                     /locus_tag="VBD85_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO7132675.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            37780..38379
                     /gene="recU"
                     /locus_tag="VBD85_000097"
     CDS             37780..38379
                     /gene="recU"
                     /locus_tag="VBD85_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO7132676.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            38366..40612
                     /locus_tag="VBD85_000098"
     CDS             38366..40612
                     /locus_tag="VBD85_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7132677.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNGN
                     NNTVPNGN"
     gene            complement(40658..41992)
                     /gene="pepC"
                     /locus_tag="VBD85_000099"
     CDS             complement(40658..41992)
                     /gene="pepC"
                     /locus_tag="VBD85_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO7132678.1"
                     /translation="MSKLTQTFTDKLFADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDLSDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(42105..42926)
                     /gene="nadE"
                     /locus_tag="VBD85_000100"
     CDS             complement(42105..42926)
                     /gene="nadE"
                     /locus_tag="VBD85_000100"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO7132679.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            complement(42923..44383)
                     /locus_tag="VBD85_000101"
     CDS             complement(42923..44383)
                     /locus_tag="VBD85_000101"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO7132680.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKRAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIEM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            complement(44541..45455)
                     /gene="trxB"
                     /locus_tag="VBD85_000102"
     CDS             complement(44541..45455)
                     /gene="trxB"
                     /locus_tag="VBD85_000102"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO7132681.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            complement(45524..45748)
                     /locus_tag="VBD85_000103"
     CDS             complement(45524..45748)
                     /locus_tag="VBD85_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO7132682.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIIWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            complement(45870..46613)
                     /locus_tag="VBD85_000104"
     CDS             complement(45870..46613)
                     /locus_tag="VBD85_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7132683.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            complement(46613..47416)
                     /locus_tag="VBD85_000105"
     CDS             complement(46613..47416)
                     /locus_tag="VBD85_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7132684.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPRHLTDEIPGGVR"
     gene            complement(47511..48323)
                     /locus_tag="VBD85_000106"
     CDS             complement(47511..48323)
                     /locus_tag="VBD85_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7132685.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKENGTLARLSKQYFGGDYVSNIDK"
     gene            complement(48461..49804)
                     /locus_tag="VBD85_000107"
     CDS             complement(48461..49804)
                     /locus_tag="VBD85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7132686.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELGTQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVDEKRRKERRASNRAKGRAERKAKKQSF"
     gene            complement(49902..50912)
                     /locus_tag="VBD85_000108"
     CDS             complement(49902..50912)
                     /locus_tag="VBD85_000108"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO7132687.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLIVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFYLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            complement(50914..53172)
                     /locus_tag="VBD85_000109"
     CDS             complement(50914..53172)
                     /locus_tag="VBD85_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7132688.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAIIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPGLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            complement(53176..53502)
                     /gene="ftsL"
                     /locus_tag="VBD85_000110"
     CDS             complement(53176..53502)
                     /gene="ftsL"
                     /locus_tag="VBD85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO7132689.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            complement(53517..54464)
                     /gene="rsmH"
                     /locus_tag="VBD85_000111"
     CDS             complement(53517..54464)
                     /gene="rsmH"
                     /locus_tag="VBD85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO7132690.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGIYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDNAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKTISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            complement(54547..55800)
                     /locus_tag="VBD85_000112"
     CDS             complement(54547..55800)
                     /locus_tag="VBD85_000112"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO7132691.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVVKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASESDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            complement(55810..56613)
                     /gene="proB"
                     /locus_tag="VBD85_000113"
     CDS             complement(55810..56613)
                     /gene="proB"
                     /locus_tag="VBD85_000113"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO7132692.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     RGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            56754..58139
                     /locus_tag="VBD85_000114"
     CDS             56754..58139
                     /locus_tag="VBD85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7132693.1"
                     /translation="MSREYITLSKNIIKHLGGQNNINNVYHCQTRLRFSLNDPTKVNL
                     EQLKTLKEVKTVVISGGQHQIVIGTHVAKVFEEINSLIETNSTTKIEQTKKAKAVSRI
                     IDFVSGTFQPILPALSGAGMIKALLALLLVFKILTPSSQTYILLNLFADGVFYFLPIL
                     IAITAAQKLKANPILALGTVVMLLHPNWANLVASGKPVSLFHTIPFTLTNYASSVIPI
                     ILIICVQAYIEKYLKQIIPKSLRLVLVPMLIFLSMGILSFSILGPMGTIAGQYLAVIF
                     TFLSKYASWAPAFLVGAFAPILIMFGVHSGIAALGITQLAKLGVDSIFGPGMLCSNIA
                     QATAGTVVTLITKEKKLKEIAGPAAITAYMGITEPILYGVNLPKRYPLIASLIGGGLG
                     GLYAGIMNAHRFAVGSSGLPGLFLYISHTSTHLFITMLIAVIITVSTTAILTFILAQY
                     YEKQVSIRQRD"
BASE COUNT        18069 a        11626 c         9266 g        19225 t
ORIGIN      
        1 ataaaatgat aatacaatat taggttcgct taagaactca tttagtatac tataattttt
       61 tatttgttgt caataggttt taaaaaaatc tcagagaaaa ccctgagatt ttttaaatta
      121 tgttacaaag ttaatttcct ttagcttcaa ttaaacctag ttcgccatct tcacgcttgt
      181 aaaggacatt tgtcgtatta tcttctgcat ctgtatagat aaagaaatca tgacctaaaa
      241 gttccatttg caacaatgct tcctcaacat ccattggttt tagattaaca ttcttagtac
      301 gtacaattct ttggcttact gcttcttcat ctggctcagc ctcaaattct gttgtgaaaa
      361 cttgacttgc tggaatcttt tcacgatatt ttttcgcaat tttagtttta tttttacgaa
      421 tttgacgctc aattttatca acaactaagt caattgaccc atacatatct tgtgaaacat
      481 cttctgctcg taaagtaata gaatctatta agattgttac ttcaactttt gcggtcttct
      541 ctctgtatac tttgaggttg actctagtat ctaattcttg tgcttcatta aagtattttt
      601 caactttaga gagtttggtc tcaacatact cacgaatagc ttctgttact tcgatgtttt
      661 caccacgaat actgtattta atcatatgag tacctctttc ttgcgttgtt aacgctttct
      721 atactcttat tataaccgct ttcatgaaaa tttgcaagat tttagcgtgc taatgaaaaa
      781 ctttttatat tttttatacc tttttcttct aatagtttcc tgatttgtgc tattgttgcg
      841 cctgttgtat aaatatcatc aacaattaaa aggttgtccc ctaactcagc ctcatttttt
      901 aatgtaaatt gctgaacttg tttgagtctt tcttctttat tattagccga ttgcttacct
      961 ccatcttttt tagaaagaat atttttgtaa ggtatatttg ccgattgtag aaaagcaatc
     1021 acttgattaa attgcctatt ttggtatcct tcatgactca agggaactgg cactatagtg
     1081 tagcccttat acttttttag agcagctttt atttcttttg taaaaacatc tttcaacaaa
     1141 taatcccctt gaaatttaaa aagagaaaaa tattttttca ttgcttcatt gtatctataa
     1201 agggaaaaat gatttacctc tatacctttt ttaccccaat aaatacaatc ttgacattgt
     1261 ttttggggaa tttgcttgca acatgtttgg caacccatct ccctactaag tgcttcaaaa
     1321 ctacccttgc acgtttgaca aattacgttt tgctttggtt ttagaagcat aagttccaca
     1381 aaagttaatt gcgataaatc tatttcatga cataacaaac aagtcattaa aatcctccta
     1441 tatgattcat gtttttaatc tcctttatag cttggtgcat cgatttactt ttcccatcat
     1501 gaaggaagta aaggagccct tcgggccttt ctaatgctct tccaactctt cctgaaattt
     1561 gtaccaagga acttttcgta aacaaatgat gatttgcttg tataacaaaa acgtcgacgc
     1621 tagggaaagt tactcctctc tctagaatgg tagttgacac taggatagac aatttgttat
     1681 ctcgaaaatc ttgcactaac tttaacctag atgtgctctt acttgaaaca aaaccaattt
     1741 tttccatagg aaaggcttgt cttaaaattt cggtgaatac ctgaccttct gaaatatggg
     1801 ggaaaaataa taacaaagga tatctcgttt gtcgctgttt atttatcaac tgatataatt
     1861 tgggaggtag tttttgtgtc tgcaagcttt tctggatacc acttacccaa accatctcgg
     1921 gtatcactaa cggattggca tgaaatctcc tagctagatg caattttttt agttcttttc
     1981 ttgcaacttt tctttctaac tcagtcgttg aagttgcagt cagaaaaata cttgttccgt
     2041 tttcctttaa ggcctgtttt acaccctggt atagtatcgg gttgtcgaca tacggaaaag
     2101 catcaacttc atcaacaatt agcaaatcaa aagcatgata aaattttaac aattgatgtg
     2161 ttgtggcaat agttaaaggg gatctctgat aagaaggaga ttctccatgc attaatgtta
     2221 tggcacatct aaaatcgttt gaaagtcgct tatacaattc taaacaaaca tcaattctag
     2281 gactggcaat acaaacgctt cctccagtat caattacttt ggcgacagag tggtatatca
     2341 tttctgtttt accagcccct gtcacagcat gaacaagaag attttcctta cttgaaatac
     2401 ctttcaacaa ctcttctgaa acctcatttt gataaggtgt cagttcccct ttccatttta
     2461 aagaattcgt tttagaaaat gttttttgtg ggaaataata taaattttca ttactagtaa
     2521 ctcttccaaa aactatacat tctctacaat aataatttcc agttggtaat tggcaatttt
     2581 tagcaacaga actagagcat cgggtacaaa tatagtcgct tcctttcttt ataaaagatg
     2641 gcaagctaat tgcaattttt ctaagttgct cagataattg tgcttttgtc caaagcctac
     2701 ctaaataatt ttccatatta tttcattcgg atttttttgc taaatttgaa gaatttttgt
     2761 agaatttctc tatgaactat aaaacgataa aatcagacgg aattgtcgaa gaagaaatta
     2821 aaaaatcgcg atttatttgt catctaaagc gagtagagtc cgaagaggag gggcgtaatt
     2881 atattacgca aatcaagaaa gcgcactaca aagccaatca ttcgtgttca gctatggtta
     2941 ttggcgaaaa aggggatata aaacgatcta gtgatgatgg agaacctagt ggtacagcag
     3001 gtatccctat gctaaccgta cttgaaaagc aaggcctgac aaatgttgtt gccgtcgtta
     3061 cacggtattt tggaggtatc aaattaggcg caggggggct tattcgtgcc tactctggta
     3121 gcgtagctaa taccatcaaa gagatcgggg tagtagaagt taaagaacaa attggtattc
     3181 gtattcaatt aacttatcct caatatcaaa cttttgacaa cttcctaaaa gagcaccacc
     3241 ttcaggagtt tgaaacagaa tttttagaag ctgtaacctg taaaatatac gttgacccta
     3301 aggaatttga acacacaata actaatttga cagagttcta ccaaggaaaa gctttgttaa
     3361 ctgaagaagg gagtcaaatt gttgaaattc cctttaaggc tgaaaccgat aaataatagc
     3421 tatcacttga taaagaattg atattttact ttaatcaatg tgagttttat aatattctta
     3481 accaatatca tgaagaggta tagcttatgt caaaaattta taattctatt acagacttaa
     3541 tcggaaatac acccattatt caattacatc atattgttcc agaaggagct gcagaggtgt
     3601 acgttaagtt agaatccttt aacccaggtt catctgtcaa agatcgtatc gctctagcta
     3661 tgattgaaga tgcagagcaa aaagggattc ttaaagccgg agatacaatc gtagaaccta
     3721 catctggtaa tacaggtatc ggtttggctt gggtaggaaa agctaaaggg tataatgtta
     3781 ttattgttat gccagaaacc atgtcaattg aacgtcgtaa aatcattcaa gcctatggtg
     3841 ctcaattagt actaacacct ggtagtgaag ggatgaaggg agctattgct aaggcaaaag
     3901 aaatttctgc agagcaaaat gcttggctac ccctgcaatt taataatcaa gccaatccag
     3961 aaatccacga aaaaacgact ggtagggaaa ttatcgagac ttttggtgaa aaaggtttag
     4021 atgcttttat cgcaggtgtg ggaactggtg ggacaattac tggtgtctct cgtgcattaa
     4081 aaaaagtcaa tcctgatgtc gctatttatg ctgtagaagc tgatgaatct gcaatccttt
     4141 ctggtgagca acctgggcct cataaaatac aagggatttc tgcaggtttc attccagaaa
     4201 cattggctac agattcttat gatcatatca ttcgcgttac atctgacgat gctattgaaa
     4261 caggacgtat catcggcgga ctagaaggtt tcctcgcagg aatttctgct tcagctgcta
     4321 tttatgctgc tattgaagtg gccaaacaac taggaaaagg gaaaaaggtt ttagctcttc
     4381 tccctgataa tggcgaacgt tacttatcaa caagcctgta tgattttgaa taactttaat
     4441 cttcagtaaa cgataactaa aaaagattag atatgttgtc tgatcttttt tagtttatat
     4501 ctctttaaat actagtaagc cctatttagt taaaatatta aaacgacaaa tagaaccaag
     4561 caatttcact tggttctatt gtttttattg atgatttgaa acataacggt caaccgttaa
     4621 cagggctgca gcgacatcct ctgcactaat tttgaatggc atttcatgta ttgtttcacc
     4681 ttcaattgtt gcttgctgac caacttttaa aagttcttca tatgttgcat cacccaaatg
     4741 gagttctgca agtgtagttg gcattccaat cgcttggtaa agattaatgt agcgatcaat
     4801 ttcttccttt ggtctatttt cgaggaaaag ttgcgtcaac gttccatagg caactttttc
     4861 accatgggtt aagtgatgaa tatctccact caaggcagta aaaccattat gaatagcatg
     4921 agctgcagct aatcctgcac tttcaaaacc taaaccacta agtaaggtat tagcttcaat
     4981 aacattttct aaagcttttg taaccacttt ggcatcacaa gccgctaaag cttgtaaact
     5041 atcattaaat aaagtacgct cacatgcttg ggcaattgcc acgcccgcta aagtctgtct
     5101 accgccagcc atcgctatcc catttttttg taagactgct ctcgcttcaa cccaagtagc
     5161 taagccatct gctatcccag aagctagtaa acgttttggc gcttgggcaa ttacttgagt
     5221 atcaactaaa actaagtctg gattctttga ataaaaaata tatttttcaa aagctccttc
     5281 atctgtatag attactgata acgctgatgt tggggcatct gttgaagcta ctgttggtgc
     5341 aatgaccact ggtaaattaa ctttatctgc aattgccttt gcagagtcaa tagtttttcc
     5401 accaccaagt ccaataataa ggtcattatt cttttctttc gcaacagcaa cgacacgatt
     5461 gatttcatta tcagatgctt ctccgttaaa ggaaacacga tgtggtaaca aatcgtcttg
     5521 tagataagat aaaaaccttt ctccaacgat attataaaca acatcatcac aaaggataag
     5581 tggagtctga cctaaagatt tgatatgttc aatactttgg aataaagcat cctttccttg
     5641 tatatagcgc gatggacttg cgaaaacttt cattctcaac ctctttcttc tttaaaaaat
     5701 tttatgttaa agatacagcc tcaaaagttg gcgttctctg ttaaatatgt tctgagtgtt
     5761 gactggcttt ccaatcatct gcaaagtcgt taacagcttt tgcaatagat ggcatcgcaa
     5821 agactgattc aaaaatatct ggtcccgcgg tgattgattg agcaccctgt gataaggcct
     5881 tcgttacttg tgaagcgttt ttaaaactag ctgctaaaat tttgctagaa gaacctgtcc
     5941 gctctattgc ttgcgctaat tccttaataa cttgtgtcgc atcaatatca aggttctcca
     6001 ttcggttaaa gtaaggtgct aggtagtctg ctccagctga tatagccaaa agaccttgca
     6061 tactggtgta tattgctgtg gctgtaatat tataaccttc tgcttttaac gttttaattg
     6121 ctgctaatcc atctggcgta actgggactt tgatataaat gtcatcgtcc gtttcttggc
     6181 gtattttagc agcatcatcc aatattcctt gataatcttt ggccactact tgaacatgca
     6241 aacttgcttc acgtccaata atttctcgaa catcacgaat gcgttggaaa aaatgtatat
     6301 ctccttcttt cttagcaatg gtgggatttg aagttacacc tgctagtggc agtatatgat
     6361 gccatttttt tatagcttct aaatttaaag tgtcaagtaa aaattccata ataatctcct
     6421 aaagagcatg ttctgtacgt gcaataatat catcttgagt tgccttagat aggacattaa
     6481 agaatgcaga ataacctgct acacgcacaa tcaaatcgcg gtgtttttca gggtgcttct
     6541 gagcatcaat caaagtctca cgtgagacaa cattatactg gatatggtaa ccatgaaggc
     6601 gattgaagaa tgttctaagt aaggcaatta atttttgttt atcttcttct tttgccaagg
     6661 tttgagggtt gactttttgg ttaaggagta caccgccgac aatctcatca gttggtaact
     6721 tcgaaactga tttcaaaaca gaggtaggtc cctttttgtc catattatga gatggtgaac
     6781 aaccttcagc taatggagtt cctgcatgcc taccatctgg agttgctaaa gtacctcgtc
     6841 cttggcctac atttgctgaa atagaggacg ttcctgaata gcgaatacca ccgattggtc
     6901 cacgtccata gcgtgtgttg ggatatttag caatttcttc aacataaaca tcataacact
     6961 cacgtactaa actatccgcg tagtcatcat catttccata ttttggtgct tcattgatca
     7021 acatttgacg aatttcttca ccacgcggac ctgcgtaatc actctgcaat gcttgccata
     7081 cttctaatgt ggttaggcgt ttttcttcaa atactaattt tttaagcgca gcaagtgagt
     7141 ctgaaagatt agcgattcca acttgtagac cagatatgta gtcatatact gcaccacctt
     7201 cttttaagtg cttacctcgt ccaatacaat catcagttaa agctgaacaa agaatatcag
     7261 gtacttctct ttcgagacta atatcaatag cattttcaac aataacgctc attcgagtta
     7321 agtaacgaag tgttttgtcc caagcttctt taagttcttt atatgaggtc atctgggtga
     7381 aatgcccata agaaggcgca aatctcttcc cagaagccgg gtcaataccg tcattcattg
     7441 taattaataa cactttaggg aagttgatat agctcatgcc ggtacaacga taaccccatt
     7501 tcccaggaac tgctgtttcg acacaaccaa tcgctgaata atcataggca tcttcttcgg
     7561 aaactccttt tttaataaat gatggaataa taatttcatc attattaaaa gctggcatac
     7621 caaagcctaa tttcataact tcaatagctt cattcatgaa tgacttgtct aggttagcat
     7681 gataacgaac cgttagatta ggctgtggta agtgtgtctg agcgactgat tttagtacta
     7741 gaaatgataa aggattaaca gcatcttcct tatgtcgtgt ttgtcctcca atcgttacat
     7801 tttggtaaag aggactacca gcagacgaaa acgtatgagc ttggctacga accttattaa
     7861 tagttattgt tttaatccat aggttagtta ggcgctcaac gatcgaatcc tcagtctcac
     7921 gtccagcttc taagtcagat ttaacatatg gatacatgta ttgatcaaag cgtccataag
     7981 atagagaatg accgtttgat tctatttgaa gaatacactg aataaaccaa accgattgaa
     8041 cggcttctgc aaacgtttca gctgggtaat agggcactcg ttcacaaata ctagcgatat
     8101 ctaacaattc ctgacgtctc ttagcatttg ctgttttagc ttgttttttt gctaggatag
     8161 caaaacgctc tgcataggtt ttaacagctt ctattgtaat taaaatagaa tcatagaaat
     8221 gatatttgtc aatactctca ggctttgtta aatctaagtc agctttagct tttcttgctt
     8281 ttttttcaaa gccaataagc ccttcttcta gtaaattctg atagttgaca gctaagtgag
     8341 catccccaga attcatctta ccttccatcc caaagaagcc tgtctccata taaacttgaa
     8401 cttcttctgg aagcataacg cccgcacgag cccgtaaatt attattttcc cagaaaggtg
     8461 caatatttct aatttgttct ttggtttctt ctgtaatgta aaagacatca ccatctcgtt
     8521 tttcaaaaag gtctagttca tttacaacaa attctagagt gtattctgga aaaataggag
     8581 catctttatc agatgaagct tggttaccga caatcattgt ctcatcgtca atatagattg
     8641 tcattttttc caaaatattt tgcaacatgt aagctctctt taaaacatta ggtttttcct
     8701 gatgcttttg ataggcttca gttaccaaga tagcacgttc tgcatctatg aatggtttct
     8761 tatcaagaac cttatcgcga taagaataca tgcgctctgt caaatagcca aaatgctttt
     8821 gagaattttg ttgaacatca tttgtatatt tttgagtgag taattgtttg gtcataacag
     8881 cctccgcttg tttctttcga aactaaaaag tttttcttcg taaatataat agcacctaaa
     8941 tcctaaaata acaagaagaa aataaagaat agaatgctac atctaaagtt gcacaaatgt
     9001 gcatttttag aaaaaggtta aggtgcatta cacacctcaa cctcttatct gctagctatc
     9061 ccctttccat ttcacattca tttttgtaag taatattatc ttggatttta aagaatggga
     9121 agtaaacagc agttgagata gctaaaatga ctatttggac gagtgcacct tgccaaccac
     9181 ctaccatgaa tccagaaata attgctggag tactccatgg taaagtaaca cctgagaatg
     9241 gttgcataaa accaaccgca atagctccat aaacaattaa agctgccaaa actggtacta
     9301 aaataaaagg cagaaacatc acaggattca ttacgattgg aaagccaaag acgattggct
     9361 cgttaacatt gaaaattgcg ggaaaagcgg ccactttccc aagtgccttg tattgttttg
     9421 attttgctgc aaaaagcatc gcaataacta gtccaaaagt tattcctgag cccgataaaa
     9481 tcaagaaact atccaaaaac tgctgtgtta caatgtgagc gccattatct aatgttaagc
     9541 gatttgctgc taataaagac ttattggcat ctaaattgga tagtaacaaa gcagtcacaa
     9601 taccattcac cacagattgg ccatggacac caaaccacca taagaatgaa ataaagaaag
     9661 caatcccaat tgctccataa agtgagcctg ttaagccttg caacggtact tgaatgatat
     9721 cataaatcat ctcaataaag gtacctccag ttgttacctt agcaatcaaa taaacaatca
     9781 tcgataaaag aaagataaca aaagctggaa tcatagcttc aaattgtttt gctattgcct
     9841 gtggtacttg ctctggcatt tttataacaa tatgatgttg gataaaccat gtatagatag
     9901 caccaactac taagccaata ataattgctc caataatccc ctgaccgcca aaccaaacct
     9961 tagaaatagc atccgctatt gcttctcctt ttacaggaat ataagatgat ttcattaaaa
    10021 taaagaaaga cgaaagtgat aaaacgccag caggtaacgg ttctacacta ctatttttag
    10081 cataagcata agcaattgca aaacaggaaa tcaagcccat aatcgcaaaa gttcctgaat
    10141 aaacttgcat aaatggttct gtccattctg gtccaaaaac attagctatg gcttgattaa
    10201 gacctttaaa tggaagctgc cctaatatta aaaagagact cccaacaact gttagtggta
    10261 gaattgctag catgccatct ttgagtgcaa taatccctcg catattgaca aacttcataa
    10321 tcggagtaat tattttctga ctatcaaact ttgacatatc aagcctcttt tcttgtttcc
    10381 aataaactca aagcaagttt caaaactttt acaccatcca acataccata atctgccatt
    10441 ggaataactg ctatcggtac accgtattca tcacagattg attttgattt atctagagta
    10501 tagctaactt gtggaccaag taatgcaaca tctaaatctt tagcataatc tgcaattttt
    10561 ccttgtgaat aagcttcaat atgtgcctct atcccttctt tatccgctgc gattttcata
    10621 ttgtttacta acatacctgt tgaaaaacct gctgcacaga ataaaccaat ttttatcata
    10681 tttgatcccc tttacttatt tttgaatatg taattcctgg cgtaaatcaa taatttcttt
    10741 aatcaagtta atctcagtaa tacttgtcat caaatgatcc tgagaatgaa taaagagtgc
    10801 tgagatatct gtccttgtcc ctcctgcttc ttgtgcaagg aattgtgttt gaagattatg
    10861 agcttctagg agtgcactat ctgctagttc tatttcaatc ttactttctg caaaatttcc
    10921 ttcttttgca agttttaagg cttgatagat atgttgttta gcatccccag cattcaaaat
    10981 gagccccata ataatttggt ctgcgactat gatttccata agcacctccg ctttaatgat
    11041 tttttataag tttaaacttt cttacaaaag gttctagttt tctttcgcaa aacaaatgta
    11101 acatgtccct taatcatcgt caatagactt tgtgcccttt atgacaatag cgcacctttg
    11161 tgcttattct ataacaattc acttggtaat agaaacatta tcaaaagagc taggatttat
    11221 tcctagctct tttgatatat agtatccaca aaggtgtctt tatctaatct taaaatattt
    11281 aaggctgtgg aaaaatcagt aactaaatga ttaatatacc ccttttttaa aacggctaag
    11341 agtgccgcag ccttataatt gccatgagca accgctattc tattaggaac tttctgtagt
    11401 tgttctaagg taattcctat tgtcctttta gctaaatgtt gattcacagg atcacctgaa
    11461 tgattaaaaa aacgacaagt aatttcacct acagctgctt cttcatccaa gcattgaaaa
    11521 tcatcttgat ttaaaaggtc aagccactgt tgttcattgc ttttaggttt accaccaacg
    11581 cccacaattg ccacatcaag tttttcccaa ttatccatta gcccctcaaa atattttgaa
    11641 gttaatattc ctttcgctaa attagcgttt tcttgaacta gagttgcatt aatgaaagta
    11701 caacttcctt ggaaacgcct tgacatttcg taaaccagag tattaacgtg atagcgtgca
    11761 ttaatatgac ttggacctcc ggccaaagga acaaaagaaa cttgagaaac cggatgagga
    11821 cgcattgctt caactacttt actaagtgtc ctcccccaag caatccccac cttgtcattc
    11881 tctttaattt tttttcgaat catagctgca gctacatgtg ctaaatcctt ctctatctct
    11941 gaagtatcgc tatcttttcg cgaagaaaca atctcaatat ctttaaggtg atatttactt
    12001 ttaaaatagg attctaactg aaaaatttct ggattaaaat ctgaaatttc gattctaaca
    12061 agaccttctt ctctcgcttt ggctaacata cgacttattg ttgtacgata aattcctaat
    12121 tcattggcaa tctcagattg gcttttccct tccatatagt aaaggtaagc tactttagct
    12181 aacaattttt gacgctctaa tttcatactc ataacctttc agcatttgct tcagtttaac
    12241 aaaaaggtta catctgtgca atttgacatg acactttatc tattatcgcc acaaatgtgc
    12301 aaaatgtaag aaaaatagcc tcttttatgg tacttgtaat aaagattgaa tggtaatctc
    12361 tgcttcttgg aacaaattag caatttccga atttggatgt ttatctgtaa taacttgtga
    12421 tacagttgac aacggaagct gttccaccaa tgcagtctga ttaaacttac tagaatctgt
    12481 taaaataatg acttcatcgg cagcattcgc catctcttgt gcaacttcag aacgcattaa
    12541 atctctaccc ataaatccta gttttggatc aaaaccatca gtcccaacaa aagctaacga
    12601 gacatgaaaa agtgaaatca tctttttcaa gagaggacct accgtaacct gtgaacttga
    12661 ttgatactca ccaccaagta agacaatttg gcatgaatca tactctcgta aatagttagc
    12721 aataaagcaa gagttggtca aaatagtgac atttcgcttt gtctgacaga tattttctgc
    12781 taaaagagca caagtagagc ctgattctat taagatagtg tcattgtcag acaccatatt
    12841 tgcagctaag gcagcaattt cttttttagt tttatggtta aaagataacc taacattaag
    12901 gtcatcccca ctgtttaaaa ctgcataacc gtgctctcta tgcaaaagcc ctttgccttc
    12961 tagtttatct aaatctttac gtatcgttac tttagaaact tgcaacaact ccgagagtgt
    13021 attaacatct attttttggt actgggatac taaactaata atattttcta atctattcat
    13081 gaaacacctc ctttctattt tatcattttt tatttattaa acaagttcaa taaactttca
    13141 ttcgtaattg actttgttac gtataaaagg tatacttaaa atgaaattga ggaaaataat
    13201 atgacaaatg aaaaaggtat tgtattcaac attcaacatt ttagtattca cgatggtcca
    13261 ggtattcgaa caaccgtttt cttaaagggt tgtcctctta gatgtccttg gtgtgctaat
    13321 cccgagtcgc aaaaaatggt tccagaaaca atgagagatg ccattactaa tgaaagcgtc
    13381 atcgtgggcg aagaaaaatc ggttgatgat attattgaag aagttttaaa agatattgat
    13441 ttttacgaag aatctggtgg gggcataacg ctatctggcg gtgaaatatt tgcacaattt
    13501 gaatttgcca aggcaatttt aaaacgtgct aaatcactgg gaatccacac ggctattgaa
    13561 acaactgctt atactagaca tgaacaattc atagatctta tccaatacgt tgattttatt
    13621 tacaccgatt taaaacatta caatagcctc aaacaccaag aaaagacgat ggttaagaat
    13681 gcttcaatca tcaaaaacat tcattatgct tttgccaacg gtaaaacgat tgtgctacgt
    13741 attcctgtca tacctaattt taacgattca ttagaagatg ctgaagaatt tgcttgttta
    13801 tttgatcgcc tagatatccg tcaagtgcaa ctcttacctt tccaccaatt tgggcaaaac
    13861 aaatatcaat tacttaatcg tcaatatgaa atggaagaga tcgctgcact acacccagag
    13921 gatctactcg attatcaagc tatttttagc aagtataata ttcactgcta cttttaaata
    13981 tttaaaacct agacttgtca ttaatcaaat ctaggttttt tgtttattta ttattcaagt
    14041 aggctagacc ttcctcaacc caactaggta aattttcctc aagtggtttg aaacctatct
    14101 taagacgatt gttagaaaat cgatgacgat gctgaatatg atgtctctct tcttcaaggg
    14161 tccgaagcga taaacttgct ttttgtgtaa attcatctac atctataacc tgaacataga
    14221 cttcatcacc tacactaaga acatcataaa tattatcaat gtaacctgtt ttaatttcag
    14281 aaatatggat aagtccagtc ctaccatcct caagagaaac aaaggctcca taaggcctaa
    14341 tgccagtgac tgttcccttt aacttatcgc caatcttcat tttattcttt aacctcaatc
    14401 gttaaaataa caacatcttc caatggtttg tcttgagaac cagtctctac tgccgcaatc
    14461 tcatcaagta cctcaaaaga actttggtct actaattgtc caaatacact gtggcgacga
    14521 tctagatgcg gtgtaccacc ttgtccagca tacaactcag caattggtgt tggccaacca
    14581 cctcgctcta attccttttt cgcataagga atttttgtat tttggacgat gaaaaattgg
    14641 ctgccattcg tatttggtcc agcattagcc attgaaagag ctccgcgaac attatataat
    14701 tcctcggaaa attcgtcttc aaaactttcc ccatagatag attcaccacc catacctgta
    14761 ccagttggat caccaccctg aatcataaaa tctggaataa tacgatggaa gataatacca
    14821 tcataataac cttgtttagc taaaccaata aagtttgcta ccgttttagg agcatgatcc
    14881 gggaagagtt ttacagtcat atccccgtga tttgttttaa tgacagccac aggaccttct
    14941 gtattttcaa tatctacttg tggaaatgtt aattcttttt ctatcaaacc tagttcctcc
    15001 agggcataca aaatgccatc ttcttctact ttttttgtga taaaatctgc ttttttttga
    15061 gcttctgggt gtgacactcc catcgccaca gctagtccag cataatcaaa gagttctaaa
    15121 tcattgagct catctccgaa aaccaagata ttctctggct ttaagcccaa atgttcaaca
    15181 actttagaaa cacctaaagc cttcgatgtt ccttttaaca caacatctga agaatgatca
    15241 tgccaacgta tcaagcgaag atgttcagct aacggttcag gtagatgaag gctatctcct
    15301 ttatcttcaa atgtccacat ctgataaata tcatgttctt tattgaaatc tggattaact
    15361 tcgagatcag aatacacaat atcaatcgca tcatttacca aatcatcacg atctgataaa
    15421 actgcctcat gacgaccagc taaaccgtac ttaataccaa cagtgtccgc ccatttctta
    15481 tattgctcta catattcagc tggaatagga cgatggaaga taatattctt atccttgtcc
    15541 ttaacgtaag ctccatttaa ttttacacaa taatcggcat ttaaatcttg aacttctttt
    15601 ggaacgccat atcgtgctct tcctgaagca atacctacaa cgattccttt atctttcaaa
    15661 gctttaaaaa cttttaagat tgatggtggc atataacctg tatctttaac acgaagcgtg
    15721 tcatcaatat caaaaaacac tgcctttatt ttttttgctt tatatttagt ttttgcgtcc
    15781 atgactaacc actttcatta acatagtgcc tctattatat cacttattca tcatcttgtg
    15841 gtaccaaatg gtgttggaaa gcatagacaa ctgcttgagt tcgatctgct acatttagtt
    15901 ttcccaaaat attagaaaca tgagttttaa cagtctttaa cgagataaat aattcatcag
    15961 caatcctttg attgtcataa cctttggcta ataaattaag gatatcacgt tcccttgctg
    16021 ttaagccctc atgtaaagct ggacatttat catgagcctt aattttctta tctacttcat
    16081 tttcaatagc ttgttctccc cttgaaactt tacgaatggc attaagaatc tctgctgcgc
    16141 ttgaggtctt taacatatag cctttggcac ctgcttcaat aacagggtaa attttttcat
    16201 tatccaagta agatgttaag actaaaatgg cagcttctgg ccaatccttc aataatgcta
    16261 aggttgcttc tacaccgtcc atttcaggca ttactaagtc cataacaaca acgtctggac
    16321 gtaactccag tgcttttttt atgccttcta aaccatttga ggcctcacct attacttcaa
    16381 catcagcttg taagtttaaa aaacttttta aaccgagacg aaccatctca tggtcatcaa
    16441 caagtacaat ttttatttta tccattttta tcctcactct gatttactaa aggtaatctt
    16501 atatccatag caactccttt accaggctga ctgagaagct gtaaatttcc tgctaaatct
    16561 tctactcgat cttcaatatt ttttaaccca taacttaaat catagacaga atccatatca
    16621 aatccaatac cattatcaat catttttaac tgcaattcat tctctgtttg attaaggtaa
    16681 acttcgagac gactggcttg tgaatgcttt aaagtattac tgataaattc ttgaccaatt
    16741 ctaaaaatat tgtcttcaat ctttttgggt aaggttagaa ttgattcatg ataaacaact
    16801 tcaatgtcgc tcttatcggt caattctttc aatatcattc tgaatccttc agatagggtc
    16861 ttattttcta attcaactgg cctcaaatga agtagcagaa ttctcaaatc attttgtgca
    16921 tgctgaagca tttcttctac tgctaataat tgcgagccaa cctgatcaac gtctagctga
    16981 gaaacattct gtgctatccc tgacaaaacc atggaagcag cgaacaaatc ctgactaact
    17041 gtgtcgtgta aatcacgtgc aatacgctta cgttcttgtt tcacaatttc ttggcttttt
    17101 aaaatacgac tattttcctt agattgtaga cttgctgtca ttaaattcat ttttttagag
    17161 agccgttgaa tattggtcga tatttcagta ccatcatcgg taacatttat tgattggtta
    17221 tttaaaatgc gacgtaaatt atggttaatt tgacgtttac tattatcatc taataaaaac
    17281 caagttagca ataaaaggat agttaccgct aaagatagaa agaaaataga gaaaatcaat
    17341 tgttcaatca gccacaaacg agacgtttga tagaggtgca ccaaattaac acccaaacta
    17401 tcaataatga caaaacaaat ggcaaatata atgacacttc catagaagaa agctaaaaag
    17461 tagtggtgct ttttcattta cgactaacct caactttccc agcgatagtg tttacaacta
    17521 atttgacacg tttcaaaagg taatattctt cctcttgtga caattttata gattcatttc
    17581 ttagatcata ctcctcaaaa tcaaagtact gtacactacc gtaaacagaa ctaatatcag
    17641 cctttacggc aacgtctaaa ggaaccaata ctttagtatc accaaaaact ttttgaatta
    17701 taataacatt atcctgccca gatacaataa catttgttaa atcaattgta tcagtcccag
    17761 agattcttat aatattaata tcttcaaaag cataaaagtc actttcgtgt tgatcagtac
    17821 ctaaccattg gtttcgtgtt gtcttaacat ctaattgatg attcttaaat tgaatcaaag
    17881 cataacgatt ttttttctta acttgcgaga aatgattgat tagaacatac aaaactgcaa
    17941 aaacaacggc tgcaataatg taagggttaa gcataaagat taaaaatagt aataacaaac
    18001 ttgtcgttaa cagaaaatta tgacgactat cgttgttata aaatcttaag gctaaaagaa
    18061 tcaaagcgag aataaaaata aaacttgtcc agtcatcaga taaaattttc attaatccca
    18121 taactaagac aacggcttca acaagtagaa aaaactgaaa ttttttcata ataatccttc
    18181 cattactcta tatgacgtat tttatcaaaa agccctatac tttgcaccat tattgagaga
    18241 aaaatttggc atattggcag agaacctttc taggcatggt ctctgccata gcaaattaaa
    18301 aagaagactt taacatcttc tttttaattt tattgagtta atggattgtt agctgttcct
    18361 gtattagttg tactatcact tggtagtgtt gaactacttg tagaagtgct actactatca
    18421 gaagtgctag catccgttga tgagcttaca gaatccgagg tagttgttcc tgttgagctt
    18481 gaagccgaag aatctattag cgtagatgat gagttattcg ttgttttaga accaagaaga
    18541 tacagcttaa ctttatctcc tctccttaat ccaacttctc ctccgtaata aggatcctga
    18601 cttgctatct tacttgaaga tggaggtgtt actactgttt gataaacgcc attttgcatt
    18661 tgatagaaga caagattatc tgcatctaat ccgaggtctg tcaaggtttc tattacttct
    18721 ccaacagtca tacctgttac atcaggcata atcattggat cattaacagc aaccgtaagt
    18781 gttaatttct tattaccatc aggattaaaa gatgttcctt ctggaagcga ctgtgctaag
    18841 attgtaccag tagtataatc attattaatt ttacgcttaa ccttgattaa agatttttta
    18901 acacctttgc cttgaagatc tttgaggata tctttgtaat tatgttcctt ataattacct
    18961 agagtgaaac tcttgtttgg agtggcaata taaagattaa ctttagcccc ttctctgcga
    19021 gaacgtcctg cagcagggtc tgtttttacg acaagtcctt ctgaaacact atcactctca
    19081 atattacgaa tagctcctac tttcaatcca gaattttcca atgtcatttt tgcttgagct
    19141 actgttttgt tgctaacatc tggaacacga atagtcgtag gacttactaa aattttataa
    19201 gcaaatacaa ttattcctac aacacagagt gcaaagaata atttcatgat tgtaccaaga
    19261 agtcgatgag aagccttctt tttagacttc gttttatcgt tactcgcaga atcaggagtt
    19321 attttttctg ttgttttggc ctgtttttga ttacgcaaca gttgttctgt cgttagagat
    19381 gatactgtag atgttacttt gggtagtgtc ttagtactct cagtatcatt aaaaacaagt
    19441 tttggttcac ggtgacgtgt cgagcttaga gcagttgata aatcacgtcc catctcataa
    19501 gttgtcttat agcgatctgt tagcttttta gctgttgctt taatgacaat gttttccaaa
    19561 gcttgtggaa cgcttttatt ttctgcaagg attgatggta gtggtttttg gaagtgctgt
    19621 agggcaatag taactgcact atccccatca taaggaatat ggccagttaa catttcaaaa
    19681 agcatgattc ccatggcata aatatcactc tgaacagtcg cttttgaacc acgcgcctgc
    19741 tctggcgata aataatgaac actacctaac atagaattcg tttgcgttaa actagtttca
    19801 gcaaacgcta ccgcaatacc gaaatctgta acttttacag tccctttttt agttaataaa
    19861 atattttgag gttttaaatc acggtgtacg atacctttct gatgtgccag tgacatggct
    19921 gaaaggactt cattcatgat tcgaacaact tcattatttg ataggggtgc attgtcttga
    19981 atatactttt ttaaatcgaa tccatcaaca tattccatta ctaagaactg ctgtccgtct
    20041 tcttcaccta tatcacgaat agcgacaata ttaggatgtg tcagctcagc cattgctcgt
    20101 gcttctctct ggaagcgagc aaccgcaatc tgatctgttt gataattggt tcgcaatact
    20161 tttattgcga cctcttcatt atctaaaatt agatctcgag ctagataaac atccgccatt
    20221 ccacctcgtc cgattgattt aaggatccga taacgaccag caaataattt gccaatctga
    20281 atcattaaac cgcctcactt tcaacgtaaa ctagtgccac agtgatatta tcaagacctc
    20341 ctctatggtt tgctagagtg attaaatctt gattcttatc atctaaggtt ttttcttgtg
    20401 ttagtactgt agcaatatcg gcattactca acatattagt tagtccatca ctattaacta
    20461 caaggtagtc accttcttca agtagatgaa ctcctaaatc aggttcgact ggattggctt
    20521 gaccaattga ttgcgtaata atattttttt gaggatgact agcagcttct tcttctgtta
    20581 attggccagc ttttaccaat tcattaacaa gagaatgatc actcgtcaat aaatgatact
    20641 ctccttgacg aacgataccg atacgtgaat ctcctacatg tgcgaaaata atattatctc
    20701 ctacaatggc aactgcttca atggtagttc ccataccttt gtaatcatct gactgaccta
    20761 attcatatat tttgcgattc tctgtttcaa tagagacaag catccaatca cgaatttctg
    20821 ataactcaga aaaatctgtc tccgcccaat cacttcctag atcagttaca gtcatctcgc
    20881 tagcaatatt tcctgcacga tgtcctccca taccatctgc taagatgatt agtgggacgc
    20941 cggctttatt ttcaaattga ttaataaaat cttgattatt ggaacgacgt tgcccaatat
    21001 ctgttaaaag agatatttcc atatattttt ttctgacgat gtcagtttcc tcctagacac
    21061 tacaagatac gtttgacttg tccaataaaa aagccatctg tatgatattg ttcagggctt
    21121 atactaatac agcctctctt tacaatatcc tcttgtgtat gactcagttc tacttgctca
    21181 aaattggggt ggttttctaa gaatttttca ataacttgaa aattttcttc ctcaaaaatg
    21241 gtacaagtgc tataggttat tataccacct ttacgtagcg tttgacaaac gctagatagg
    21301 atagacaatt gaatctcttg gagtgcttca aagtcttgat tagctttatt gtacttaata
    21361 tctggtttac ggcgaattag cccgatacct gaacaaggtg cgtctactaa aattttgtca
    21421 aaagtatcct caagaaaata ctcatgagct ttacttgcat ctaacttttt agtttttatc
    21481 ttatctgaca gacctaggcg ttttgcattt tccataacca attccaattt atgatcatac
    21541 aaatctaaag cagttactgc ccctgtagtc aaatatgagg cgatatgact tgtttttcct
    21601 cctggtgcac tacatgcatc caatacttgg tcattacctg agactttgag tgttggagca
    21661 actaattgac tagattcatc ttggatagta atgaggccat cttggaaaaa tgatgtcgat
    21721 gcaaaatttc ctgagtctgc aactaaagct gtctctgcaa tatgtgaatc tctaacattt
    21781 aaagtttctt tgatagtttg tttttgcgat aagtcagtca cacgaagact ggctttattt
    21841 cgctcaaata aactctccat aatagctaaa gctcttgtct caccatattg gtcaattaat
    21901 ttttttacga gccacactgg catggaataa gcaacagagt aacgtttgtt ttggcgtttt
    21961 attgatgcaa tttcagggag cgtctccgaa cttactcttc gtaaaacggc attaattaac
    22021 ttttcagcac cctttttatt gcctcgattc ttagcaatag tgacagcatc attaacaata
    22081 gcgtgatctg ggatgttgtc cagatagagt agttgataaa ggctcagtag caagagatga
    22141 tatacccata attcaagctt atcacgatca actataaagt gtgatagata ccattctagc
    22201 gttatttttc tagccacagt tccatagaca atttctgtta ctaaggcacg atctttatct
    22261 gatagtctag atttttttaa actcttatta agtgcgatat tagaataagc tcctttatca
    22321 aaaacttcct ctaaagtcat taaagctaaa ccacgcgcac tctttttcca atcattcgcc
    22381 aaatttatct cctatttcta aattacgacc aacaccattt aaaaagtctt ttatatccat
    22441 acgaggtttc cctgctggtt gaactgattt gagttcaata gctccgtcac cagttgctac
    22501 tgtcaatgag ttctttgtct tagcgatcac ttgacccggt gagccctttc cttctgacat
    22561 tgttacttca taaagtttaa aacgatttcc ctccaaaagg gtatgtgcaa ctggccatgg
    22621 atacatccct cgaacatggt taaaaatgtc gcgcgatgat ttattccaat caattctctc
    22681 ttcatctgga ctaatattag gagaaaaact tacctcttct tcattttgtg gtatcggctt
    22741 aatatcacct gatagataac ctggaagcgt atctaataaa aggtcacgac caacaacggc
    22801 gagtctatca aacattgtcc ctacattgtc ttcatcagtt atctcaacgg aagcttttga
    22861 taccatatcc cctgcatcca tctttgcaac catttccatg atggtaaccc cagcttcttt
    22921 ttcgccatta ataatggcat aatgaattgg tgccccacca cgatattttg gcaatagaga
    22981 agcatgtaca ttaattgcaa aacctaccga ttccaaaagc tttgttggta agaattgacc
    23041 aaaagcagct gttacaatac catctgcacc tagggtcatt agttgttcta attctggact
    23101 tccagatagt ttttccggct gatacacagg aatgttgttt tccaaggcca cttcttttac
    23161 aggggtcatt ttaatttctt ttttacgacc tacagcacga tctggttgcg tcacaacagc
    23221 taaaacatca tacttaccat ctgctaaaat accttttaaa accgtcgctg aaaaatcagg
    23281 ggtccccatg aataataatt ttgtcattgc tttttcctcg tctttctttt ctcaattata
    23341 tcatagcact attctacatc atattttgcg gttcgtggtc tattgctaag cgaagatctc
    23401 tattctcctt gtcttgagtc atgtctaata agcgattcaa aacaagttca aggttgtcct
    23461 caaatcgata cttgataata atttggtagt gataaaggtt atgtgttctt gcaattggtt
    23521 ttggcgttgg acctaacaac tttactttat cactaaagcc ttgcttcaat aaacttaata
    23581 cttcataaga ttttcgtatt aaccactctt catctttatg cgataatgtt aaaccaactg
    23641 taaaataata aggaggataa ccaagttgtc gacggatatt catttcatat tgataaaaag
    23701 cttcaaaatc ttgcttttgg gctaattgaa tagcataatg attaggatta taagtttgaa
    23761 tgacaacctc tccttctttt tcagcacgcc cagcacgtcc agccacttga gttaatagct
    23821 gaaaagttcg ctctgatgat cgaaaatcag gtaaattcag agaagtatct gcatttagta
    23881 ctccaactaa agtaacattt ggaaaatcca atcccttagc aatcatctgt gttcccaata
    23941 aaatatcagc ttcatgattt ccgaaacgct tcaaaataga ctcatgaccg cctttttgcc
    24001 ttgtcgtatc aacatccatc cgtaaaatct ttgcgtctgg gataactttc aaaagttctt
    24061 cataagcttt ttgggttcca gtaccatagt aagagatact tttactattg caatttggac
    24121 atgttcttgg aataggcttt tcaaacccac aataatgaca attcatggtc tttgtagcca
    24181 tatgtaaagt cagagaaatg tcacaattag gacattgatc aacgtagccg cagtctcgac
    24241 acataataaa actagaataa cctctacgat tgagcataag aaccacttgc tctttcttat
    24301 ctaatctatc tcggatttta tctagtaaat atgatgtgaa attactaact tcttgttgac
    24361 caataaaatt tctaaaatca ataatttcaa cctgaggaat acgtgcttta ggattagcac
    24421 gatgttttaa ttctaaaaat ttataaacat cccgactagc tctagctcgg ctttcgatac
    24481 ttggagttgc tgatcccatt agtaacacag ctttataata ttctgcacgt agcaaggcca
    24541 catcgcgcgc atgatacctt gggttagatt cttgcttata agttgattca tgttcctcat
    24601 ctataatgat ggctccgata ttttccaaag gagcaaaaat tgctgaacga gctccaacga
    24661 caactttagc ttgacctgat tttatctttc gccactcatc aaatttttca ccttctgaca
    24721 agcccgagtg cataatagca acttgctttc caaaacgaga aataaaacga ttcgtcatct
    24781 ggggagttaa agaaatttct ggaacgagaa caatcgctgt ttttcccaac tttaaaacgt
    24841 tatcaatgat atgaagatat acctctgtct taccagaccc tgtaatcccc tccaacaaga
    24901 agggtttatt ctgttcctta ccaatttggt caacaactat tttaactact ttagcttgtt
    24961 cctgatttaa atccaaaaaa tccgttttta ggattccttt aaaataatta tcagatctac
    25021 tagcttcttt ttcaatgact tcaatgattc cttcttctct gaaaaaatta accacaggag
    25081 atgaaaattg tttgtttaaa gctgtgaggc gccctggttg aggattttct aacaggaatt
    25141 ctttcaactc cagtctcttt ttcgctcttg ctgctatttg gctcttttct aacagagttc
    25201 tgttgatttt gtaaatcttt tctgttttta ttgttttctt atctttggct aaatattgaa
    25261 cttctatgaa acctttttga attaatctgc ctgctttttt ggcatcttga gaagacaaac
    25321 ttgaaaagac aatttccgtt ttatgaccaa atagatcttc tctatcttct gatggtaatg
    25381 tgtctgttgc taacaagagt ttatcatatt gcgaattcaa aagacttggt aacattgatt
    25441 tcaaaataga tattttataa gaaaagacag tatggcgcat ctgatcagct aagtctagtt
    25501 gttcctgatt taaaacaggt tcaaaatcta ggacctccgc aatatcctta gtctctaatt
    25561 catcatcatc tctgaaaccg acaacaaagc cttgcaaaag acgatttcct cttccaaaag
    25621 gaacatgaac gcgtactcct acttgtacca aatcttccaa atctttagga atggcatacg
    25681 aaaatggttt atctgtctgc atcagaggaa tatcaacaat aacctgtgct aattttcttg
    25741 ccataaaccc tccctaattc tctaaaatat gaaaagaggc tgatacagaa tgtttcaacc
    25801 tctcactttt gacttgctta aattttttcg ccgtcttctt tttctttagc aatttgctct
    25861 ttgattttac gttcttcttc ttctttagca aggcgttcag cttcgatacg agcgcgaact
    25921 gaggcacgtt ttgctgatgg atctgggtga attacaacat tacctgactc gatttcttct
    25981 aaagcgcgaa gtgttgattt cacagattta aaatcttgag tggctttctc accagcttcc
    26041 aattcatgag cacgcttagc ttgtaaaata actaaagaat attttgaagg aactttatcc
    26101 aacaaagtgt cgatagaagg ttttaacatc ataaggcaat tctcttttct atttttaact
    26161 taatttttta tcagtttctt tgaccatgtt ggtataacgc ccaataacac gatccacacg
    26221 ataatgttct gcttcaatga cacgtttaac acgttctgct gctaatgaca cttgatcatt
    26281 gacaacagca tagtcatact ccctcatcaa agcaatttct tctttggcac gttcaatacg
    26341 ttgggcaatc acttctggac tatctgtccc acgtcctact aaacgctctt ctaattcctc
    26401 taaatctggt ggtgttaaga aaatgaaaac accgtccgga accttcgatt taacttgaag
    26461 cgctccctga acttctattt caagaaaaac atcaatccct ttgtctaaag tctcgttgac
    26521 ataacttaaa ggagttccat agtagttacc aacatactcc gcatactcaa gcatttgacc
    26581 ttctttaatc aacgcttcaa actcttcacg tgtacggaag aaataatcaa ctccatcaac
    26641 ttcgcctggg cgttgtggac gagtagtcat tgaaacggaa taatcaaatt tatggtcagg
    26701 ggttgaaaaa atttcttgcc taactgtccc cttaccaaca ccagatggtc ctgagaaaac
    26761 aattagcaaa ccacgttcag acattacaca ctcctcaaat taatagatcg ggtgttaagt
    26821 gttaaaaaga ccagaacaat ctggttctta ccttaactat taacttttat agcaattcat
    26881 aaattgctcc ctaaataaag tttcttctca ttagtttaac aaaaagaaac ctttttttca
    26941 aggtctccta ttatttttta tagagtcttt ttaccccacc tattccacta cttgaattag
    27001 taaaatcctc aggagctctc tctccaattt ggataaaaag cttgatataa gatggtgcct
    27061 caatgccctt tttagcatag tattttcctg ctctagagcg acgccaaaac ataaagagcg
    27121 gtatgactaa atatgcaaat atccctacct caggtagaaa ataagcgagg ataatgatga
    27181 taaagcgaac cacaactgaa cgaatggcaa aagttgtcat gacttttata acccgctctt
    27241 tcaattcact aggaatggtc actaaatcat aacttgccaa acgaatccga ataatgtctg
    27301 atgtgagacc aactaataac gttaagagtc cataagccat aacagcaaaa ttatgattct
    27361 caaaagaaat cataaattta gtaaagagag gaatcaaaca aatacctagg tgttcaatga
    27421 tatcaagagc tataatttca tcattagtct tttgagcatc taggctattt tgtgcccttt
    27481 cgtaccaaaa attaaaaaca acaacaaagc taacaataaa taaacctatg ttccgtgtaa
    27541 agtcaccaat atcacccatc gtagccggag tttttatctc caaaactaaa atcgtcatag
    27601 caatggccaa aattgcatca ctaagcgtat caaaacgttc tttaaatttt tgcatacata
    27661 catccctctt aatacattat ggcatctcga ctggaaaaaa actatcacag ccaatccttt
    27721 tctcgtgcta ttttaagtgc ctccaaacga ttattaactc ctaatttatc aaaaatattt
    27781 gacgtgtaat tacgaactgt accattcgat aaaaataaag tgcatgcaat ttctttgctg
    27841 gtcttccctt gagcaattag ggaaagaatt tttatttctt gctcactcaa aggattagat
    27901 ataacgtttt ccattaattc aggcgaatat tctttacccc cagataaaac tttttgaatc
    27961 gttttcatca aatcagctac tgagcggtct ttcaaaacat aagcatctac atttgaacga
    28021 atagcccttt ggaaataacc tgaccgctta aaggttgtta caattatgac cttaacattc
    28081 tggtattttc taacccactc taagacatct aaacctgata atatcggcat ctcaacatcc
    28141 aatattgcaa catctatggc tttattggat aaaatggcaa tagcttctcc accatttcca
    28201 gcttgatcaa tagtagaaac actttcttcc atcaaaagta attggcacat agcatctcgt
    28261 agcatggact gatcctcagc aacaagtaat ttcatcttat acctctttca ataccacctg
    28321 aatatgagtt ggtttacttc gggacaaaat cgttagtgtc ccttcaacga tagttaatct
    28381 ctcttgaata ctatgcaact catctccgtc aaggtttgta aaaccacaac cattatcaat
    28441 catctcaata tttatagtag caccttgcct tactaaactc aactccacgc tatcagcttc
    28501 agcatgctta acaatattat tggataattc ctttaatatc attgtgattg tagattgtgt
    28561 tactggactt agttgtgacg tctctaactt attaacaact gttaacttaa tgttagataa
    28621 ttgaaacaag cggtacaact catcaatttc ttccactact gtacgatact tgaggttaga
    28681 aacaatttga cggacctcac tcatcgattg atgactaata tgatttaatt ctgatagttc
    28741 tttttgtacc ttatcatagt tccttttttc taacaacttt aacgctagtt ctgtctttag
    28801 tgtcatcata gcaaatgtat gtcctaaact atcatgcaaa tcacgcccta tacggttacg
    28861 ttcattttca gctgacaaaa tattgatata ctgattttgc tctgcaattt tttctctttg
    28921 ttcttcgcta tttctatttt caatagcaat ccacatgtaa ccgatacaaa aaataggaac
    28981 aattagaaac atgattctat caccaaagtc tgatgcctta aagaagcata aaaataccac
    29041 tgtggctaga gtacaagcaa aactaatgaa tctaaaagac ttcaatttat ctctaaagcg
    29101 atagacgagt aaattactca gaaagtaaat ataccagatg aaaccactat ttataaaaat
    29161 tgtcatgtag aaaatatatg tcaacattgt agaccaggca atcatgtgat actttgattc
    29221 gcccctgata ataacagagt ataaatatgc tcctacaaaa gctagcgtaa aaaataaagt
    29281 ccaaaaaggg tagtgataat agagaatccc tcctatgggg taaatcaaaa atactaacgg
    29341 aataaaatac attaaatcat agttattttt tttatttttc aaattgaatg cctcttctga
    29401 gatagtagat aagcgataac taacattata ataccatagc ctatcagaat aataagagaa
    29461 aaaccagcaa aactattagc aaacggacta aggataactt gagtcaaatg gtaagtaggg
    29521 gttaactttg ataaagcttg caaccatttt gggaacattg taattggcat ccacatgcca
    29581 ccaagaacag ctaatgacat ataacaaata ttggctacca ttgacattag ttcagtattt
    29641 ttaaagtagg agacgagcaa tccaaaagga ataaaacatg ttgctcctcc taatagcaat
    29701 aaaaagctct gcatccattg ttctattggc atactaactt gccttaaaat atgcccagtt
    29761 aggaaaacaa cagttattgc taggcaatag taaaataaaa tacgaataat ttttgctaga
    29821 taatattgcc acattggtac tggtgagtgt cttaataatt gcattcggtt tgagagttga
    29881 tcatctttca tagaaaatgg aaaagtaaaa aaagcaaagc taagacttga gaatcctgcc
    29941 atcgttatca tataattacg aacgtagata gcttcgatgg ctttagtgag tccttcgccc
    30001 ataaatcctg aaaaaaatag aaaaaaagcc actggcatac ctatagccat aatgagattg
    30061 gcaagttgac gttttgtcaa aagaaattcc attttcaata atgctttaaa gattctcatg
    30121 ttttacctcc ttagtagtct caaaaatacg atttaacaaa gttctattgg tcatctcaat
    30181 agcttcaata ggacaaccat tatctgccag tattttccat acagcttcag cctccccagt
    30241 aacaaacgtt atactatcac tcttggcttc acactctgta atcaaatagg taggtaataa
    30301 tttttgataa ctaagcggaa cggtgaataa cttttcggtt ttttcttgct tcatggcaaa
    30361 gggggttgta tcgcgtaata actttccttt atgaagtact aaaatcctat cagctgtatg
    30421 ttctacctct tcgatataat gactagaata gacaattgtg acaccttctt tttttagtgt
    30481 cgcaatcagc tcccaaaatc gttgacgtgt ggaagtatcc attccggaag taggttcatc
    30541 taaaaatagt aattggggct taccaaccaa agttaaaaca aaagctaaaa gtcgtttttg
    30601 ccctccagat aaagtctctg taaattgttg caagtgcgta tcactccact gtagatagtc
    30661 taaaatcaga gacattggca gcacttctgg aaataaacac ctttggaaat caatgagttc
    30721 tctcactgta aactttgagg gcaatacatt ttcttgtggt aagatggcca ccttatattt
    30781 taacctattg tggtgtgcag gcagattaaa aatcgtcaat gatcctgaag aaatactaat
    30841 atcacctaac aaagttgaca tcagagttgt tttccctgca ccattaggtc caatcaatgc
    30901 tacacattgg ttagaagcta tactaaatga tatatcttct aaaatcgttt tttgcccgat
    30961 gcttttaaat atatgatcaa ccttaattat atcttttgcc atttggttct cctttactat
    31021 aatcatagta taaaaaatca gaagtaaact ttttagatat ttttgtcatt tcaaaatatg
    31081 acaaatgtca actctataac aaaaggagag ctaacaagct ctccttttga ctattttgca
    31141 aaatcaacag ctcgcatttc acgaataact gttaccttaa tattacctgg ataatccaag
    31201 ttttgttcga ttttttcacg taccttatgc gacataataa ctacttgatc atcagaaact
    31261 ttaccaggtt gtaccatgat acgaatttca cgtccggctt gaagtgcaaa ggcattttgc
    31321 actccctcaa agccattagc aatttcttct aaatcacgca gacgtttgat ataattttcc
    31381 attgactcat tacgcgcacc tggtcgtgct gaactaaggg cgtctgcagc agcaacaatt
    31441 actgcaataa cgctgtcagg ttctacatca ccatggtggc ttgcaatagt attaacaaca
    31501 atcggatgct ctttgtactt acgagcaaat tccataccaa tttcaacgtg actgccttca
    31561 acttcgcgat caatagcttt ccccatatca tggaggaatc cagcccgacg agctaaatca
    31621 acattttctc ctagttctcc agccaaaata cctgcaagtt taccaacttc aactgaatga
    31681 cgaagtacat tttgaccata tgatgtacgg aactgcaaac gtcccataat ttttatcaaa
    31741 tcaggatgca gatttggtgc accaatctca taagctgctg cctcaccata ctcacggata
    31801 cgttgatcca tttccaaacg atttttttca accaactctt caatacgagc tggatggata
    31861 cgtccatctt gaatcaaaga ttctaaagtc atacgagcaa tttcacgacg aatggggtcg
    31921 aaacctgata agacaacgac ttctggcgta tcatctatga taacatcaat acctgtcaaa
    31981 ctttccagag tacgaatatt acgtccttca cgaccaatga tacgtccctt catattatca
    32041 tcagggagat gaacagttgt aattgtttgt tcagtaacat attcaccagc taaacgttgc
    32101 atagcttggg ctagcaagtc ttttgctatc ttgtttgatc gatcttttac ttcacgttct
    32161 gcttctttaa tacgtgtcgc aatatcatga gctaaatctt tttcggtatc agctaaaatg
    32221 atatctttag cttcttcttg actaagctct gcgatgcgtg acaattcttc tacttttttt
    32281 gtttctaaag tagcaatttc ctgttcgcgc tcattaatat gtctagattt atcggttaga
    32341 ctttgttctt tgctatctaa cattttttct ttgttagata gattttcatc tttacgatca
    32401 agagatgatg cacgatctgt taaacgtgcc tccatctgtt tcaactcttg tctatcagat
    32461 ttaaattcct tttcgatctc ttcacgatat tttctagcct cttcttttgc ttctaaaagt
    32521 aattcttttt ggtgagcttt actttcacgt tccgctgctt tacggatgtg ttctgcttca
    32581 atttctgctt taccacgtaa atcaacggca tcttgttctg catttaaaag agttagttct
    32641 gcagcttctt ttgatgattt cattttcatt gaaatagcca cataaccaat aatcaaacca
    32701 atgagtgcac aaaccattgc taatataata ttaaacatgt ttatacctca atttttttct
    32761 agatttcata tagaaagtcc acttttaagt ttatcataaa aaaggcgttt tcacaattta
    32821 aaattataga catttatgat atgattagaa tgttatttaa agggattagg agaaattttc
    32881 aacatctcct ttagtctcta gaatgtaata caaaaggaga aaacttatga caaaagaagt
    32941 tgtcgtagag agttttgaat tagatcacac catcgttaaa gcaccctacg ttcgtttaat
    33001 ttcagaagaa gttggaccgg tcggagacat cattactaac tttgatatcc gccttattca
    33061 gcctaatgaa aatgctattg atactgctgg tttacatact atcgagcacc ttctggcgaa
    33121 gttaatccgc caacgaatta atggtttgat tgattgttct ccttttggtt gtagaactgg
    33181 ttttcacatg attatgtggg gaaaacagga tgctactgaa attgctaaag tcatcaaatc
    33241 tagtctagag gctattgctg gtggggtcac ttgggaagac gttcctggaa caaccattga
    33301 atcttgtgga aattataaag accacagtct ccactctgct caagaatggg cgaaattaat
    33361 tctttcccaa gggatttctg acaatgcttt tgagcgccac attgtctgat ctaataaaaa
    33421 ataaaagtca acttttggaa ttccaaaagt tgacttatta atttaaacaa aggattttgt
    33481 tctgtatcat agagagcaaa atccttttaa tttttcccta atatcctcat agcagtcgta
    33541 gacatctaaa tgcgaataca aaaaacaccc taatcgttag actgattttc taacttttag
    33601 ggtgtagaca aaaaactggt tttgttcttt atattaataa tctaaactat cagctcttcc
    33661 aggagcgtta cctacaaagt aacctgtctt ggcattgata gagaacaaat aagtgccatc
    33721 taatttaaac atattgtaat aaccattacc attgataata tttacaggtt caagaatata
    33781 gttatttcca gagaaatagc ctcgcgcctg tgaagtggcc acgattttct ctaatactcc
    33841 tgggttaaat atccaggcag gatttcctgt atccgcaata gcagcattat tatacggtac
    33901 tcgactacgg ttacgttcca aattacctga tgcagtgcca gctgcctgat tatttattgt
    33961 agcagctgat gaggaagatt gaccgttagt tacgtttggt tgttcagtta tttgagtgtc
    34021 tgaaacctta ttagacgaag cctgttcttc ggattgtttt acctgattac gaccagttga
    34081 aacgatggag tgaagtaaac tatctagact agcattacca gtattaagag ttttactatc
    34141 taaagagtca aaattcgctc catcttttac ctctaatttt tcactcttct taccatcaac
    34201 tacagcaggc gacttaaact tgtcgttaac agctttaaca gcatcaattt gacgttttag
    34261 actatcaaat ttaactttta ctttaccgta gtatttagtt ccttttaaag catcaacttt
    34321 cttttctaat tctgacaatt tatcaaattc gctatttttg agttgggttt tcttatcatc
    34381 aacaaaaaag ttagcataag atttgttgaa attatctaag ttctgactag cttttttatc
    34441 ttcttctgat gaagtcgtcg tagccttaga actagaactt tgacttgttg cagtttgaga
    34501 atttgtcgaa ttattattct tgaaatagtt gtaaccaaag attgctacta ttagaagtaa
    34561 aataagagca atcaatgttc ctacgatacc tccacgcttc ttagaagagg atttatactc
    34621 ctctgaatca gcaatagtcg aatctggttc tgttgcagtt ggttctgctt ccgtattcag
    34681 aggaggaact ggggttacac tattttcttg ttcttgtgaa gattcttctt taggtgagac
    34741 agcattagct tctgtatctt gttcttgaga gactgaatta tccaactttt tagaaagctc
    34801 ttctgcagct aacatcgctg ataattcttc gcgctgtttc ttgataaaat catctaaaga
    34861 tgccgtatca aggtttgcaa aatcgcttga ttttgtctca aacttttgtg aagctacttc
    34921 atcacgatgt tgtttgatgt atttatcaag aatactatct tcttcggtaa taccagcctt
    34981 aatttcagaa tcttttctaa cagcctcttc tactgtcatt tctttagcat ctttaaattc
    35041 aagaccatca gtttcttttt ttggttcaaa aaatgggtgt ttttgatctt ctgacatgag
    35101 ttctccccgt tattttactt gacgtctcac acgttcaccg taatattggt acaaatcaac
    35161 ttttaaagta ccattgtaaa gcttacgttt tttatcagct tgtgagccgt attttttttc
    35221 aaatccttcg tcactagtta ggataaactt gctccaagtt tttaaaggcg caaatgtttg
    35281 tcccatctca ttatacaaaa tgtccactgc tttgtcatcc aggagtcgtt caccatatgg
    35341 tgggttggag ataataacac cgttaatttt atctgttttt aaatcttgaa gacgcatttg
    35401 tttcaatttg ataacatctc caagtcctgc ttcttctgca ttttttctag caatttctac
    35461 catacgaccg tctaaatcaa aacctgaaat atctaaatca atatcatagt ttgctttact
    35521 ctcagcttca tcacgaacct tttgtacttg actttgatct acccatggcc acgcctcaaa
    35581 ggcaaaatca cgattaaaac caggagctat attcattcca atcatagcag cttcaataca
    35641 aaaagtacct gaaccacaag ttggatcaat caatggctta tcagggaacc aattagataa
    35701 ttgaataata gctgctgcca tgttttcttt aattggtgca ccaccttttt ctgcacggta
    35761 gccacgtttg aagagacttg atccagtcgt atcaatcatg acagtcgctt tatcttttaa
    35821 aatagaaact tctattttaa attcagctcc attttcttgt aagggaactc cttcaggacg
    35881 gtgaaagact ttttgaagtt tcttagcaac agctttttta gaaatggctt gtacactagg
    35941 ttcattgtgc aattttgact tgacacactt tgctttagca ataggaaatt ttgcccctaa
    36001 cggtaaataa ttttcccagt caaggccata tacaccctga aaaagttcct caaaagtacg
    36061 tgctggaaac tctcctacaa taatctttat acgatcggca gctcgtagcc ataggttcgt
    36121 ttcaatgatt gttttgatat ctccgtggaa acggactcta ccgttctcaa cttggcagtc
    36181 aattcctagg tttctgattt ctctaccaac aatggcttct aaaccagcag cagcagttgc
    36241 tattaatttg aatgattctt tcatttttcc tcttttacac tatcgtattc atcacagtta
    36301 aaaattggct gagatgttat ctcagccact aacctattat gcaattttct ataagccatg
    36361 ttttgttcca gaaacaacta ggaattgttt ctttcgataa tcatctgtct actgcaaagc
    36421 agtcaggata gattgttctc attccaacta tcccatgccc ctaccaaagt ttgggttgct
    36481 agcttgaggg gtttaccgcg ttccactttc tagatttccc tagaaactac gtcactgtgg
    36541 cactttcaaa gctattcagg catatccaaa gacttagccc ttttgcttgc cgtaagatag
    36601 ctagctatct ccctaggctt attttttcgc ctagcacaaa cactactggc atcacagcca
    36661 gtgctagcat ggactttcct catagtatct caatactacg cgattatcca aaaattgcat
    36721 atttgagaaa tccttattct ctaatttgtc ttccaaaaac ttctttctca aggcggctaa
    36781 tgcgttttaa aatatcaaaa ttggttgcag attgggcaat cggtttttga ttatacatag
    36841 ctgtccctga atttcttaca tttgaagctt cgctttcttt tttgtctaaa gcttgttgta
    36901 gacgtctatt ttccatttgg agcttctcaa tttgctcaag ataattttcg taatctttga
    36961 tgacatcatc cagaaataca tcgacttctt tcttgtcata tccccgcata ctaactttaa
    37021 aatcttgctc aaaaatatct tttgggctat aaataatact tgccattcta ttctccaaac
    37081 cagtcaaatc gttttaatat aagtcctatc ctttatatca aagtaaataa tacctaaatt
    37141 aaatggaaaa atcaagcgta aaacgttctc atatccgaaa aaacgatcat tcctcatata
    37201 tttcatttaa gcgatcaaaa gttaggaaag aaatatcata agcttccttt ttctccatca
    37261 tttctagcaa aaattttaaa ttagtttcat tttcagaatc ataaaaaaga tatgcacctt
    37321 gtgtgttgtt aatgagaaaa tgattgtatt gtttaaattg acccgggttt tcatagcttg
    37381 ggaaagtata tttaacaaag tcaacttgct taaagagtgc caacttagct ttattagcct
    37441 cattccaatt ttggccatgg ttctcaaagg taaaaattgt cgcaatttga aaatcatatt
    37501 ctttttgtaa atcaagagca acttccaaag cccagtactc aaaccctaaa tttcccataa
    37561 aaataaacca gtctgctcca ttttccaaga agcgtctaaa atccttatca atagcctttt
    37621 ttataatagt aatccgagga tccttatcct ggaaaatccc taattcaaaa tttttgtaac
    37681 ctgttacaag aatagttgac ttcatttgtt gtccatccta attttatggt ataatagttt
    37741 tgcttatctt agcattttat ttgtaatgca aggagaaaaa tggttaacta tccacatcag
    37801 cttattcgga aaacaacagt tacaaaatca aagaaaaaga aaatcgattt tgccaaccgt
    37861 ggtatgtcat ttgaagctgc tatcaatgca accaatgact actatttgtc tcacgaatta
    37921 gctgtcattc ataagaaacc aacgccagta caaatcgtca aggtagatta tcctaaacgt
    37981 agtcgtgcta agattgtaga agcttatttt agacaagctt ctactactga ttattctggt
    38041 gtttacaaag gttactatat tgactttgaa gccaaagaaa cccggcagaa aactgctatg
    38101 cctatgaaaa attttcatgc tcaccaaata gagcacatgg caaatgtatt acagcaaaaa
    38161 gggatttgct ttgtcttgct tcatttttcc acacttaagg aaacctatct actccctgct
    38221 aatgagttaa tttcatttta tcagattgat aaaggcaata aatcaatgcc tattgattat
    38281 atcagaaaaa atggattttt cgtaaaggag agtgcctttc ctcaagtccc ttacttagat
    38341 attattgaag aaaaattatt aggcggtgat tacaattaaa aaggaatctg ttatcaaact
    38401 attgaagtat gcttttggca ttataatggg atttattatc ttagctattg taataggtgg
    38461 gctcctattt gcatactacg ttagtcgttc tccgaaatta accgatcaag ctttaaaatc
    38521 cgttaactct agtttggttt atgatggtaa taataaactt attgccgatt taggctcaga
    38581 aaagcgtgaa agtgttagtg cggatagcat tccactaaat ttggttaacg ctatcacttc
    38641 tatcgaagat aaacgtttct ttaaacatag aggtgtcgat atttatcgta ttttaggtgc
    38701 agcttggcat aatcttgtta gtagtaatac gcaaggtggt tcaacccttg atcaacagtt
    38761 gattaaactg gcttactttt ctaccaataa atctgaccaa acgttaaaac gtaaatcaca
    38821 ggaagtttgg cttgcgcttc aaatggagcg taaatacacc aaagaagaaa ttcttacttt
    38881 ctatattaat aaagtttata tgggaaatgg gaattatggt atgagaacaa cagctaaatc
    38941 atactttggt aaagacctaa aggaattatc tattgcacaa cttgctttgc tcgctggtat
    39001 tcctcaagca cctacacaat atgaccctta taaaaaccca gaatctgctc aaacaagacg
    39061 taataccgtt cttcagcaga tgtatcaaga taaaaacatt tctaaaaagg aatacgacca
    39121 agctgttgca actccagtaa ctgatggctt aaaagaatta aagcaaaaat ctacttatcc
    39181 aaaatatatg gataactact taaaacaagt tattagtgaa gttaaacaaa aaactggtaa
    39241 agatatcttt actgctgggc taaaagtgta tactaatatc aacactgatg cacaaaaaca
    39301 actatatgac atctacaaca gtgacactta catcgcttat ccaaacaatg aattacaaat
    39361 agcatctacc atcatggatg cgactaatgg taaagtcatt gcacaattag gcgggcgtca
    39421 tcagaatgaa aatatttcat ttgggacaaa tcaatctgtc ttaacagacc gcgattgggg
    39481 ttctacaatg aaacctatct cagcttatgc acctgctatt gatagtggtg tctataattc
    39541 aacaggtcaa tcattaaacg actcagttta ctactggcct ggtacttcta ctcaactata
    39601 tgactgggat cgtcaatata tgggttggat gagtatgcag accgctattc aacaatcacg
    39661 taacgtccct gctgtcagag cacttgaagc cgctggatta gacgaagcaa aatctttcct
    39721 tgaaaaatta ggcatatact atccagaaat gaactattca aatgctattt caagtaacaa
    39781 cagtagcagt gatgcaaaat atggtgcaag tagtgagaaa atggcagcgg cttactcggc
    39841 ttttgcaaac ggcggaactt actataaacc gcaatatgtt aataaaattg aatttagcga
    39901 tggaaccaat gatacttatg cagcgtctgg tagccgtgcg atgaaagaga ctactgccta
    39961 catgatgacg gatatgctga aaacagtact aacatttggt actggtacta aagcagctat
    40021 ccctggtgtt gcacaagctg gtaagactgg tacttccaac tatacggaag atgagttagc
    40081 taaaattgaa gcaactactg gtatctacaa tagcgccgtt ggtacaatgg ctcctgatga
    40141 aaactttgtc ggctatactt ctaagtacac aatggcaatt tggactggtt ataaaaatcg
    40201 ccttacacca ctttatggta gccaactgga tattgctact gaggtttatc gtgcaatgat
    40261 gtcctaccta actggcggat acagtgcaga ttggacaatg ccagaagggc tctatcgaag
    40321 cggaagctac ttgtatatca atgggacaac tacaaccggc acttactctt cttctgtcta
    40381 taagaatatc tatcaaaatt ctggacagtc aagtcaaagc agtagttcaa catcctctga
    40441 gaaacaaaaa gaggataaga atacagcaaa tgatgccaat tcatcatccc cacaagtcga
    40501 aacacctaat aatggtaatg ctactacccc taataattcc aatcaaactg tgccaggaac
    40561 tggacatggt aatggtaatg gtaacaacaa tacagtacct aacggtaatt aaaaaactgg
    40621 atatattatc cagtttttta atttataatt actattacta attagctaat gctcccatcg
    40681 gatcccacgg cgctaaagta attggctctg cgttgtaagc ctctaattct tctttagtaa
    40741 gcaattcttt acgaacgact atttggtaag tatattcatc catccaagca tctgaagcta
    40801 caaaataacc atccttacca actttctctc cccaagagtt ctcaactttc cattttaaag
    40861 gttgtccaga ttcatcaagg tctactcccg ttaagaccat agcatgtgtc attaaacttt
    40921 cactataatc taggcggccc gctttgtctt ggctaagttt aatatccata gatgaattaa
    40981 aatcataagt cgtagttgct aggataccct tttgacgatt tgatacttga cctacgtctg
    41041 aaccaaacca cacagtttct cctgatttca tttgcgcaat cgctaactct ttaaaccgtt
    41101 tcatatcaag gtttaaatat ttaacagctg gccctccgac aacatttcca agcatctcaa
    41161 ctgtatagga ttgtccataa ggcttatcta cagttggagc attgataata gaaacataat
    41221 ctgataggtc aagattaaca tatttttgat aaaatgcttt cggtgtaata tttttatcag
    41281 actgataatg attgtcttta tcacgataag caaaatcaaa agattgcggt ggcaagccaa
    41341 ggttcattgc taaaaagtta aaaatttctt gaagcaattc ttccttttta ttttgaacag
    41401 ttgccccatc tgctccctgt gctattaatt ctcgtagaat ctgtgcatct tggcgtaaga
    41461 gtttattcaa atattgattt agttcacgac tagcacttga tgaaacagat tcaggataaa
    41521 ctgttttagg aacgacacca tatttctcaa acagagcaac caccatgtcc cattgtcctc
    41581 catcttgttg aggtacatct aataggaatt tcaccttacg actactcaat tcctgatttg
    41641 cagtcgcaat aatttgttcc ataaaccaat ttgatttttc atacttatcc cagaaaaatg
    41701 tatgtgcctg agataattca aaattctcaa gtttaaaatc tgaaatcagt ttatgacgaa
    41761 atgtattcag tgcagcaaac atccagcaac gtcctgattg tttctgatta gaaacttcat
    41821 ctttggtcaa atcaattgaa aagacatagt cgttctcaat ctcactttga cgagtctcta
    41881 gagacttcaa taaaccattg tgagttactg cattttcaat agcgctaaat tttgtatttg
    41941 cttggtaatc agcaaataac ttatctgtaa atgtttgagt taattttgac ataaattcct
    42001 ccttttatac ttagctacta ttataaacat tctgaatatt tctgcaagtc tgaaagtaat
    42061 ttattagaga aacaaaaata gctagaatat tctagctatt tttcttattt ccagaaatca
    42121 tcaaaaattg tgattggaag atgacgttta tgttgaccct tataccacca attttcaatg
    42181 atacctcgag acttgtccga aaccaccttc ccttcaagat aagcatcaat ttcttggtag
    42241 gttactccaa gggctatttc atcagcgatt cctggtttat tttcttctaa atctgcggtt
    42301 ggaatctttt cgtacaaggc tttatcagca ccaagttcag ctaataactg tttcccctga
    42361 cttttattaa gtctaaagag aggtaataag tcagcacctc catcaccaaa tttggtgaaa
    42421 aaacctgtaa tattttctgc agcatgatca gtaccaataa ccgctccagc atattgacct
    42481 gcaactgcat attgactaat cattctttga cgagctttaa tatttccttt attaaagtct
    42541 gtaatctcta ctcctgctgc attaagagct ctaacttggc catcaacagc ttctttaata
    42601 ttaatggtca aagcaatatc tggcttgata aaatctaatg ctttttgtgc atcttcttca
    42661 tcggcctgaa taccatatgg taaacgaata gctataaatt gatagttttc ccctgtatca
    42721 gcacgcaact cttcaactgc tagttgagct agacgtcctg ctaaagttga atcttgcccc
    42781 cctgaaatac ccagtacata ggtctttaga aaactatgtt ttaatagata gtcttttaaa
    42841 aattctacgg aacgacggat ttcttgactt ggattaataa caggttttac accgagttct
    42901 ttgataattt gatcttgcaa agtcatctta cttctccttt tgccaaagct tctttacgga
    42961 tacgatcaat caggtccatt ttattttgcc aaacatcgcg tgccaaatca actgggtaat
    43021 cctgtggatt cagaacacgt ttatactcat cccaaagctg atcaaattct ttgcgtccgt
    43081 actcttgaat ttcttgaaga cttggtaatt gataaactaa tttccctttg tcaaagatat
    43141 ctaccagtaa gggtactgcg tcaaaatcac ggactgtttt attgatataa gtataagttg
    43201 gatgaaacat ttcaatttca tctaattgtg tcacatcagt gtctgcaaat gtgatgtaat
    43261 caccctcaga tttcccttta gctcgactgg tgatacgcca aacttgcttt ttcccaggtg
    43321 ttgatacttt ttcagcattg tttgatagtt taattgtatc tcgcatacta ccggcgtcag
    43381 tctcaataga aacaatttta taaacagcac ctagcgcagg ctgatcatag gctgtaatta
    43441 attttgttcc gacaccccaa acatcaattt tagctttttg catttttagg ttgaggattg
    43501 tattttcatc tagatcattc gaagcataaa tctttgcatt aggaaaaccg gcgtcatcta
    43561 actgctgacg aactttctta gacaaataag ctaaatcacc tgaatcaagg cgaactccca
    43621 agaaattaat cttttcgccc atctcttttg caactcgaat agcattaggc acacctacac
    43681 gaagggtatc ataagtatcc actaaaaaga cgcaatcttt atgagtttct gcgtaagctt
    43741 taaatgcctg gtaatcatcg ccataagttt gaactaaagc atgtgcatgc gttcctgata
    43801 ctggaatatt aaagatttta ccagcacgaa cattgctcgt tgcgttagca ccaccaataa
    43861 tcgctgccct agtaccccaa atggctgcat ccatttcttg agcacgtcgt gtcccaaact
    43921 ctaatagcgg ttcatcttca ataacagaac gaatacgagc tgcttttgta gcaacaagtg
    43981 tctggtagtt gatgatattt aaaatcgctg tctctactaa ctgacattgt gctaaaggac
    44041 cttcaatttg aactaatggt tcattagcaa atacaaggtc accttccttg gctgatttga
    44101 cagtcagttc catttttaaa ttttttaagt aatctaaaaa ttcttcagga taacctaact
    44161 cttctaaata ggataaatca ctatcagaaa agctcagatt ttctaaataa cgaacaatac
    44221 gctctaaacc tgcaaaaaca gcataaccat tttcaaatgg aaccttgcga aagtaagctt
    44281 caaacaccgc tcttttattg tgaatacctt tattaaaata aacttgcatc atattgattt
    44341 gatataaatc agtatgtagt gttaaactat cgtctttata catcagacaa tctcctttta
    44401 atcatgagcc taggataagg ctatatgtct aacattatag catatttcta tctcggtagt
    44461 tcagtttatc accttacttt catgaaaata aaaagaaaga ctaatcgttt ttcgatagtc
    44521 ttttctactt ttagtatttc ttagaaatgc tcagttatat agttatagac accctgtcca
    44581 gcgatagcac cttcaccaac agcagtcgca atctgacgta gatctttttg acgaacatcc
    44641 ccaatagcat atagtcctgg aattgatgtt ttcatatttg tatcagttaa gacccagcca
    44701 gtctcgtccg taatacctaa ttctgagacc atgctagaat gtggtttcaa accaacgtaa
    44761 atgaaaacac ctccgaaagt catctctgag atttctccag tctttagatt ttcaacagtt
    44821 acaccagata ctttaatctc atttccttta atctctttga ccactgaatc ccatacaaat
    44881 tttatttttt cgttagcaaa agctcggtct tgaagtactt tttgagctct caattgatca
    44941 cgtcggtgaa taatagtaac acttttagca aattgagtta agaagacagc ttcttcaaca
    45001 gcagaatcac cgccacccac aactaaaaga tcctgatcac ggaaaaatgc accatcacaa
    45061 accgcacagt aagaaacgcc acgactagta tattcctctt cacccggaac tcctaaaagg
    45121 ctgtttttag ctccggtagc aaggattact gttttggctt catacgattc atcttcggtg
    45181 ataacacgct ttacatcacc atcgttctca actctttgaa ctataccata aatatgctct
    45241 acttcaaatt tttccaacgg ctcatacatc ttcattgata attctggacc tgagatatga
    45301 tcgtagcctg gatagttctc aatttcagca gtattattca tctgtccacc tggagcacct
    45361 tgttcaatta acccaacttt aaggtttgaa cgtgcagcgt aaagcgctgc tgtcatacca
    45421 ccaggacctg aacctattat taaagtatca tacattacta tttctccttt gaatcttatg
    45481 gttttattct atagcaaagc gtctatattt ccaactaatc tgactaagct tttatcatta
    45541 aaatgatagc tacaaatgca aaggcaagta ttggagctgt cataagagct atcagaagga
    45601 catcataaag aacagattgg cgttctctag ctgttttatc tatgtttttc ttggcacgcc
    45661 aaaagaccca aattatacca actaaaataa cagttatgct ggcaagccct aaaccttgta
    45721 aataaacttt tgctatttct aaaaacatct ctatccaaca agcgattgtc aaaacatatc
    45781 gcagctttct tatatactta gcctaactca agaataatta aaaatgaaga ttagacattc
    45841 taaccttcat tatatcaaaa tattttagct tataggtacg atttgttata actagcgaag
    45901 aattcttttg tacgttcttc taaaggatgg ttaaataatt gttcaggcgt cccagactct
    45961 aaaatacgac ctttttccaa aaataaaact ttatctgcta cttggtaaac aaaattcata
    46021 tcatgactaa caagtaccat ggtttgcccc tgcttagcag catctgcaat agatttttca
    46081 acttctccaa ctaattctgg atcaagtgct gatgtcggtt cgtctaaaag aagaacatca
    46141 ggtttcatgg ctaatgcgcg tgccaaagca acacgttgtt tctgtccacc tgataaatgt
    46201 cttggataat atttttcacg atctgcaagt ccgacctttg ccagttcgtc tcttgcaatc
    46261 cgtgttgcct cttgatctga catctttttg acaattttca aaccttcttt tacattatcc
    46321 aaagctgtac gacgctcaaa tagattgaat tgttgaaaaa ccatggctaa cttacggcgt
    46381 aatgttagta tgtcatcttt actgattgat ttaaaatcaa ctttaaaatc atcaatttca
    46441 attgttccat aatcaggctc ttctaaataa ttcatactac gaagaaaggt tgattttcct
    46501 gcacctgaag cacctactag agcaacaact tgaccttttt caatatctaa atcaagtttg
    46561 tctaacactt tttgaccaga aaatgattta gtcaattgtc tcaattttat cattagcgta
    46621 ctcctcctgg aatctcatca gttaaatggc gtggagcctt aattgccatt tttcgttcaa
    46681 tagcatttcc caattgttca attaagaaac taaccgccca gtaaacaagt gccacagaga
    46741 tataacgttc aaaatagcga taatcgctac cacccataat ttgtgcttgg gcaaacattt
    46801 caacaatacc agcattaaaa gctaaagagg ttcccttagt tagaccaatt aacgtgttta
    46861 tcaaagtagg tgttgcgact actgctgcgt ttggaataat aacacgacga taaacttgcg
    46921 ctgaggtcat ccctaaacta cgtgctgcct caatttcacc ttgatcaacc gacaaaatcg
    46981 ccgctcgtat agtttcactt gtataagccg cttcattaaa agcaaatgca gtaatagcaa
    47041 atacagaagc cggtatagca ttaatgttcc aatcaaaacc atatttttgg tttaaaaatt
    47101 tcaagaaaag tggaattcca taataactca gcattaattg aacaagtatt ggagtcccac
    47161 gtaaaaaact aacaaataaa gcttgaatcg gatataaaat tttgacacgg ttaattttca
    47221 ctactgcaaa aatcaaagct aaaatgagtc caaataaggc tcctgctact gtcaataata
    47281 gtgttatggg taatcgctct aaaattgagg gtattgcatc taaaacagcc ttccaactaa
    47341 ataatttccc cgctggaacc tgactaacca aattattata ccaagaccat ccacccgtca
    47401 agataaattg attcattcct tctactttcc ttttctaaac tttcttatct ccgttattct
    47461 atcacgcaaa ctctcacatg taaaaagata aaacttatag tctcactgat ttatttatca
    47521 atgtttgaaa cgtaatctcc accgaaatat tgtttactta aacgtgccaa agtaccattt
    47581 tctttcaaaa ctttaatacg cttatttata aatttctgta gagttttacc ttttttatct
    47641 tttggtaaaa ggaggtattc tagtccatcc ttattattac caattttacc tttcaaagga
    47701 gaaacgctta agtttaatga ttggtctttt acaatatagt cggatgaaat ggcatcatat
    47761 aggataaagt caattttccc actctcaata ttttttaatc tgctagtaac accagttgtc
    47821 ccagaaacat atttgatttt tattggtttt ttattaggat gatttttatt ccaattttct
    47881 agaacctgtg catagttaac gccagataaa acttcggttg attttccaga gaggtcactt
    47941 aatgatttgt aatggctccc cttcttccct actacggcat aatttgaacg ggatatagga
    48001 tctgagaaga gatatttttc tgctctttct ttattgtatg aaaaatcatt agctgataaa
    48061 tcaaatttcc ctgcatcaat acctgttgaa atagtatcaa aaggaactgt cttgaaggtt
    48121 actttgtact tactaccttt aaaaacagct ttgacaacat caacatcata acctttgaat
    48181 ttcccgtctt tttgataagt aaatggtgcc gtgtcagaat ctgtagctac tttaagttct
    48241 actttctctg acgcctgaac tgatactgct aggctggcgg ttgcggctat agttgcaata
    48301 gataatatta tttttttaaa cataagaatt ctcctaaaat atcatattac tagtctaaca
    48361 ctctaaaatc tatctgtata atagtatttc cttatcaggt gataaagatt ttatatcaat
    48421 aaaaaagcct gaaacgagct gatttcagac tttaataatt ttaaaaactt tgttttttag
    48481 ctttgcgctc cgcacgcccc ttagcacgat tacttgctct acgctctttg cgacgctttt
    48541 catcaacttt ccattgaatt tttttcttgt aacctggttt tatttttttc tttttctttt
    48601 tcacaagtcc aatcatttct gtatctaatt tttgatagga cttttcacgg ttattacgac
    48661 gatcacggtc gtatgtatct tgaaattcac cgtttttaat gactttagga ataaaattaa
    48721 tacctagttt ttcaagttca cgaatatcag aatcgtcact tggctgatat aaagttatag
    48781 cagtaccaga taagccatta cggcctgtgc gaccgacacg gtggacaaag aaagataggt
    48841 cttgaggtat ggcatcattg ataacatggc taacaccttc aatatcaata ccacgcgctg
    48901 ctaaatctgt tgcaacgata tattcaaatt cgaggttttt aacctgattc ataatacgtt
    48961 tacgttcacg aggtgcaatc ccaccatgaa ttttagctac ttttaagcca ttactagaaa
    49021 ggtacgaatg taattcgtct gcacgctctt tcgtattaac aaatatcatt gcaagatagg
    49081 gctgcattag tttagagagt tccaaaattt gtgcattttt atcacgtcct ttagtggaca
    49141 aaagccagtt atcgatggta tcagcgataa ctgttgctgt tttgattttt tccattacag
    49201 gattggtcaa atatttcttt aaaaatggct gtaatttttg tggaatcgtt gctgaaaaga
    49261 ctaaaatttg gacatccttg ggtaaagacc ctgcaatctt atcaacggta tcaagaaatc
    49321 ccatatctag agtcatatct gcttcatcaa caacaaaagt atgcgcctta tgaatagcta
    49381 aatcacctga cttcaccaaa tcataaattc gtcctggggt tccaatgaca atatgaggtt
    49441 ggcttacttt caacttttca atttgacgta atttgtcagt accaccaaca tagttaacca
    49501 cccgtatttc ttgctctgaa tgctcagcaa tttgtttagt tgcctgataa atttgtgttc
    49561 ccaactctct acttggtgca gtaataacaa cttgaacatc atcactactt tcatctagct
    49621 tctcaaaaat aggaagtaaa aaggtgtggg ttttaccaga accagttttc gattccccca
    49681 caagatcgcg gcctgaacga acaactggaa tcaatttagc ttgaacatca gttggatcga
    49741 cgaattttaa ctcatcgaga gccctctgaa tgtatggttt aaaattaaaa tctttaaatg
    49801 acatgtctat ttccttacta ttattatgtt cattatacca ttaaaggcta caagaaagca
    49861 aaagggaatc tggcagtcca taccacatcc cctaataaag tttaaagata taaaatagca
    49921 agtgtaatag cgctcatgaa aataccaata gcccataaaa aagcatctac tttccattcg
    49981 ctccatttat tacctttgcc agacacacca cctaattcta aatggtgatg aaatggtgtc
    50041 atgcggaaga tacgtttccc aacaccagtt ttcttcttag tatacttaaa gtatgctact
    50101 tgcaacatca ctgaagacgt ctcaaaaaca tagacaaagc cgataaaaag caaggtccat
    50161 tcctgacgta aggcaattga aatggctgct aacatagcac ccaatgcaag tgaaccaaca
    50221 tctcccataa agactttagc tggtttatga ttaaagacaa aaaagccaag taaagcacca
    50281 atcatgataa cgataatcaa taaaatatca aattgcgttt gattataagc aatgattcca
    50341 taagtaatca aactgataac aaccgaaata gaagctagac catcaattcc atctgttagg
    50401 ttcacagcat ttgaaaaacc aacaacccag aaaagaacaa aaaaagcgta taagtatcct
    50461 acttcaaggt aaaaaccaaa aatatttatc gcagatgtgc cacttggtaa cacatggaca
    50521 aaataaaaaa ttagacccgc aattagttgg agcgacattt tttgctttgg agtcaagccc
    50581 tcattgattt gtttaaaaat tttcaaaaaa tcatctaaaa aaccaataat tccatagatg
    50641 agaacaacag ataaaatacc aaaagttgca cctaaattac cactattttc tttagataaa
    50701 ataatactaa aaataagtga gacaagaaga gcaacaatca aaaataccgt tcctcccatt
    50761 gtcggcgtac ctgctttggc tagatgttgt ttgacatctt catgcatttg ttgcccgcca
    50821 attttcttca attggtaaaa cttaatgaag cgcggaatgg caataactgt caggacaaat
    50881 gctatgacac ctgccattat acttaaaaac atgttaatct cctattgtaa ttttgatttt
    50941 tttaatttta ttgatggatt ttccaacatc aatactttgt tttcttactt tacctgaggt
    51001 tgaaccttta taggtcactt ttatacctgt ccatttagca aactgttcaa cgtttgcttt
    51061 agaccaacca tacatatcag gtaattcagt cagtttattt gttaatacta gtagctgttc
    51121 attctcagca agattagctc ctggtctttt agatacttta gaaactttac taccatttcc
    51181 taaaataatt ggctgaacca gattacgacg taattcttcc gctacatgac ctggattttc
    51241 accaactatt tttgatagtt tatattttgt ctgatgttct gagtcattca atcccggttt
    51301 taatatcgtt tctttcattg ctgttgcttg ttctaaaaca ggattaacaa catctttcca
    51361 gaatgtgata ctaaattttt cgggttgttg gatggtaaca tacatgatga agtcaggatt
    51421 ttccgatggc accatagcca caactgagtt aatagtatca ttttttcctt gcagataacc
    51481 accaccacct tcctgggcga tttgggcagt accagatttt actgcaactg attgattccc
    51541 cacctgaata actggggcac ctgctgcgta taatgtacca taataaggat ctgtcccaac
    51601 agtaaccata taatcacgtg ttttactagc tgcttctttg gaaactggtt ttccaacgac
    51661 ttcttttcgt gctgtacgag aagtccctgt atttggatca taaatactgc taataaactg
    51721 tggttgtaac ataacaccat cattcgagat agacgtaaat gcgcgcaaca tttgaacttg
    51781 cgttacacca ataccttgac caaatgagct catagcgata gttacttcat tgtcagatgg
    51841 gaacaaacct ccactttcat gaagcatccc aaaacgtgtt gggtaaccaa atttaaactt
    51901 agaaagatag tttaaccatt tatctcgtcc cattttttgt tccaaggtta ccataccaac
    51961 gttactggaa tgtgcaaacc cttgtggaaa agtcatataa ctaccagatg ataacccttc
    52021 attgacatcc caatctcgaa taatcgcatc tgcaatttta tattgtgcac tgttatacac
    52081 ttcagtagag ttaaaatgct tagaatcaat tgcagaggct agagtcatta ccttcattgt
    52141 tgaaccaggt tcaaaaaagt tatcatatag tagtgtgtta taggtaccca aatttttctt
    52201 atcgtaacct ttaagggtag aaggattata agtaggacgc tgactcgttg ctagaatttc
    52261 acccgtttta gcgttgacaa ctgtagcact cgcaaatttt cctttggttt tttctgcgaa
    52321 aacatccatc tgagtctcta aaacagtctg aataggctct gataaagttg tgtagatatc
    52381 cttaccatta accgccctac gttctgttgt tgcagtacct aaaagaacat tcccactacg
    52441 gtctttttcg tatgttactt taccatcagt acctgataat accttgttaa gtgccgcttc
    52501 aaggcctgta ttaccaacta atttttttcc atctccatca tcctgaggaa gagtaaatcc
    52561 tataaattgt gaagcaaata tcccatttgg ataaatacgc cccggactgg tagaaaaacc
    52621 aattcctttg atatctgact tttccatcgt tttcttgatg tcagccatct tggtataaga
    52681 caatcctgaa cctgaacttc caaaggaaac ttgaaacaat ttcttttgat tgagttgttt
    52741 taggacaaga tttttcttca tgcccaattt attctctaag atactagcta ctttttcata
    52801 ctgagaaggt tgtacatata gtttttgacc tgtagctgtc gtatagttct ttgagataat
    52861 agcgtagaga ctataggtcg tagcatcctc agcgatagga tttccattac ggtcgtaaat
    52921 tgtcccacgc ttcgcctgta ctgtcatact ttgttgatag actttcttgg cttccttgga
    52981 taaattaacc ccaaatttac tatctgtacc aacgatgatg acaaagttga tgataaaaat
    53041 gaaaaatagg aaaatggtta aaatcatcag attttgacct actctttccc tatttttctg
    53101 cggcgaacgc ctatctctta tatggatcac ataatcaagg aagatttttt ttagcttttt
    53161 aaaaaaagtc acagactagt ccaccttccg ataaatattg tcgttttgaa ttgttaaacc
    53221 agcatcttta gcaattttag taattcgatc acgattagaa agctcattta cttcttgttt
    53281 agcattgtca aattctgtct gtttatcgtt gattttacta tttaattgat ttacttcttg
    53341 tttaacttgt aaactattac tttgcaagta aataataccg actgccatga taatagcagt
    53401 aattacaata gcaccataaa aagctttctc aatacgagag aaagtcttga tgtgacgttg
    53461 taaagtttgt gttactgctt cagttcgttt ttcatttgtc ataagtttct ttcttattat
    53521 cgaatacgac gcgctactct caatttcgct gaatgggcac gattgttcgc ttctagttct
    53581 tcttgacttg gtaagatggg tttacgatta acgagctcca tcttaggttg taaatcatct
    53641 ggtataaaag gaagaccttt aggtacttct actgttgatg cttctttaaa aagttgctta
    53701 gtaagacgat cttctaaaga atgaaaagtg atgacagata tacgaccatc aacagctaat
    53761 aagtccattg cttgttgtat agattcatct gcagctccta gttcgtcatt gacttcaata
    53821 cgaatagctt gaaaaatttg tttagctggg tgaccttttt tcttcagttc tttggcagat
    53881 ttagacgatt tgataatctc agccaattct gttgttgtag atattgtttt ttcagctcgt
    53941 acttgctcaa tttttcgagc aatttgtttt gaaaatttat cttcgccata cttaaagaaa
    54001 atccttacta aatcatgata actataagtg ttcacaacgt cataagctgt aagactctgc
    54061 tctctattca ttcgcatatc aaggggggcg tcttgtttat atgagaaacc gcgttcacgc
    54121 tcgtccagct gtgggcttga tacacctaaa tcataacaaa taccattaat ttccgacact
    54181 cctaatgctt ttaagttaga agatagattt ctgaaattat ctttgataaa ggttacttga
    54241 ccggtatcca catatttctt taaacgtata tgagcattgt caatagcctt ctggtcttgg
    54301 tcaaatgcgt aaaggtgccc atctgggcct aattgtgaaa gaagatattc gctgtgtcct
    54361 gccccaccta aagtagcatc tacatagata ccatccggct taatatcaag catatccaca
    54421 gtctcgtgca aaagcacagt aatatgatga aaatcgtttg tcataacttt aattgtacca
    54481 taaatcctaa actttagaaa agtaaaagag agaccgataa atgcaatctc tcctattata
    54541 ggtcaactat tcccttactt gaccagttcc attaatatag tattttgtac ttgttaaagc
    54601 ttctaatccc atcggtccac gcgcgtgtaa tttttgagtc gaaataccaa tctctgctcc
    54661 gagtccaaaa acgaaaccat ctgtaaaacg tgttgaagca ttaacataaa ctgccgctgc
    54721 atctaccata tcctgaaaaa tttctgccct actgatatta ttagtaatga tagcttctga
    54781 gtgatgacta ctgtatttat taatccagct aattgcttgt tcaaaagaat ctactacttt
    54841 tactgacata atatagtcta aaaattcagt agcataatca gactcacttg cagcaacagc
    54901 attttctaaa agttgcaaag cttcctcatc ggcacgaaat tcaactgact ggttcgattc
    54961 tttgagcatt ttctccaatt gagataaaaa tcccttagca atagcttgat gaacaactaa
    55021 cccttcggca gcattacaga cactagggcg ttgggtttta gcattaataa caatcttagc
    55081 tgccatatct aaatctgcaa attcatcaat atagatatga acatttccaa cacctgtttc
    55141 gataacagga atctttgact tttccttcac cgtttgaatt aattttgcac cacctcttgg
    55201 aattaagacg tccacgtagt ctgttgcctg cattaactcc tctgctacaa cgtgactagt
    55261 atcttcaact aattgtacta tatctgggtt gtgtcctgta tcttgcaatg tttggcgcat
    55321 acaattaacc aaggcagtat ttgaaaaaat agcatcttta ccaccacgca aaataatagc
    55381 gttccctgtt ttaaaagcta aactaaaggc atccaccgat acattgggac gactttcaaa
    55441 tatcattgca ataaccccta aaggcactct ttttttaagg atttttaaac catctaaatt
    55501 tgtataacca ctaacaactt gaccaatcgg atctgccaaa tctgctacct gataaatacc
    55561 gtttgctatc gcctgtatgc ggtctttgtt tagtcgtaga cggtccacca taatttctga
    55621 gataccatta tcttttgcct taacaacatc gcgagcattc tcagtcaaaa tggtttcagt
    55681 atcagccact atgtttctcg ctaagtccct gagtatctca tttttactag ctgtacttaa
    55741 tcgtgctact gattgactag cttttttagc attttgccct aatatttcaa tatacgtcat
    55801 aactcaatct cactttcctt gagcaaaata agttccaata ttatgcccat ctaacacctt
    55861 taaaatatca cgaggattgg cgccattcat caatatcatt tgtccattgt tatcaaacac
    55921 catttgtgca cttttaattt tactaagcat gcccccagta ccaaatttac ttcctgctcc
    55981 tccagctgac ttgatgatgt catcggtgat ttcagaaaca tgactacgta aaacagcatc
    56041 atcataaata ttaggatttt tatcaaaaag accatcaata tctgacagca taattaataa
    56101 atctgcttta gtaattttag caacgacagc agagagacgg tcattatccc caaacttcgt
    56161 tttatgatcc atctcatcca cgctgacagc atcattctca ttgacgattg gcaaaattcc
    56221 catagagagc agactctcaa aagagtttgt tacattttgc aaactctctg gaaaaaccac
    56281 aacgtcacgt gttaataaaa tttgggaaac atgagtttga tagtgcgaaa atatctgaga
    56341 ataaagactc atcatggcta cttgtcctac gctagaaact gcttgttgtt gtgatatttc
    56401 ttgaggacgc ttatccattt tcaaaatgtc taaaccaaac cccatagcac cagatgatac
    56461 taaaatcacc tccatgcctc tattcataag actagaaata acaaatgcta aatggtctat
    56521 tttacttaaa ttaattttcc cgcttgtctg tacgagggaa ctggttccta ctttaataac
    56581 aattcttcta gttgtctcaa aatgtctttt cataaaaaaa ttatatcata ggccctttag
    56641 aaatgctatc tgaaaacatc attgaaaatt cacaagcatt tccaagtgtt ttcttttatt
    56701 actatgctat aatctagaat cggaaaacgt tttataagta ttaaggagat attatgtcta
    56761 gagaatacat caccttgtca aaaaatatca tcaaacacct tggtggacaa aataatatta
    56821 acaatgttta ccattgtcaa acacgccttc gctttagtct caatgatccg acgaaagtaa
    56881 accttgagca attaaaaact ttaaaagagg taaaaactgt cgttatcagc ggagggcaac
    56941 atcagatagt gatagggact catgtcgcaa aagtgtttga agaaattaac tccctcattg
    57001 aaactaactc aacgactaaa atagaacaaa ccaaaaaagc caaagcagtt agtcgaatta
    57061 ttgattttgt ttctgggact ttccaaccga ttttacctgc tttatcaggc gctggtatga
    57121 ttaaggcatt gctagcttta ttactagtat tcaaaatctt gacaccgtca tctcaaacat
    57181 atatattact aaatcttttt gcagatggtg ttttctattt tttacctatc ttaatcgcca
    57241 tcacagctgc tcaaaaattg aaagctaatc ctattttagc tttgggaact gttgtcatgc
    57301 tccttcaccc taattgggct aatttagtag cttctggtaa accagtatcc ttattccata
    57361 cgatcccatt cacacttact aactatgcat caagtgtcat tcccattatt ctaattattt
    57421 gtgttcaggc ttatatagaa aaatatttaa agcaaattat tcctaaatca ctgcgtttag
    57481 tgcttgttcc tatgttaata tttctcagca tgggaatttt gtcgttctct atattgggac
    57541 caatgggtac tatcgctggc caatatttag ctgttatttt tactttttta tccaaatacg
    57601 ccagttgggc acctgctttt ctagtaggag catttgctcc tatcctaatc atgtttggtg
    57661 ttcatagtgg aattgctgca ttaggaatca cacaactagc gaaattaggg gttgattcta
    57721 tttttggacc aggcatgctc tgctcaaata tcgcacaagc aactgcaggt acagtggtga
    57781 ctcttataac aaaagaaaaa aaacttaaag aaattgctgg acctgctgcc ataacagctt
    57841 atatgggaat caccgaacct atcttatatg gtgtcaacct tccgaaacgt tatcctctaa
    57901 tagcctcact tattggtggt ggtcttggag gactctatgc cggtataatg aacgctcacc
    57961 gcttcgctgt aggctcttct ggtctaccag gtctgtttct ttatatcagt catacttcaa
    58021 cacatctttt tattaccatg cttattgcag ttatcattac tgtaagcaca actgctatat
    58081 tgacattcat tctagcacaa tattacgaaa aacaagttag catcagacaa agagactagg
    58141 ttatccctag tctctttgtc tgatgagata taatagatag caataa
//
LOCUS       DATRSD010000003        48363 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRSD010000003 DATRSD010000000
VERSION     DATRSD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48363
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.003"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..1246
                     /locus_tag="VBD85_000115"
     CDS             <1..1246
                     /locus_tag="VBD85_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO7132694.1"
                     /translation="AVSVISKTINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSF
                     IEKNDLLKEISDITIFWLIIRLIGLALGLMTVLHIGPEMVWGKETGGLILFDLIGGLF
                     TIFLAAGFILPFLTEFGLLEFVGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIAL
                     TDKQYVEGYYTSREAATISTTFSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCA
                     FIIPYIPPLSRQSNSYYTKPKKETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNN
                     ASKTVLSLWFGVMPTIMTVGTIALIISVSTPIFKILGTPFLPFLELLGIPEADIASQT
                     MIVGFSDMVVPSIMAAEIHSEMTRFIVATVSIVQLIYMSETGAVILGSKIPINILELF
                     IIFIERTIISLPIIVLMAHLFF"
     gene            1430..1564
                     /gene="rpmH"
                     /locus_tag="VBD85_000116"
     CDS             1430..1564
                     /gene="rpmH"
                     /locus_tag="VBD85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO7132695.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            1920..2594
                     /locus_tag="VBD85_000117"
     CDS             1920..2594
                     /locus_tag="VBD85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7132696.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLVFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSILKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEEMT"
     gene            2594..3781
                     /locus_tag="VBD85_000118"
     CDS             2594..3781
                     /locus_tag="VBD85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7132697.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            3792..3914
                     /locus_tag="VBD85_000119"
     CDS             3792..3914
                     /locus_tag="VBD85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO7132698.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            3927..5462
                     /gene="dltA"
                     /locus_tag="VBD85_000120"
     CDS             3927..5462
                     /gene="dltA"
                     /locus_tag="VBD85_000120"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO7132699.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            5459..6724
                     /gene="dltB"
                     /locus_tag="VBD85_000121"
     CDS             5459..6724
                     /gene="dltB"
                     /locus_tag="VBD85_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO7132700.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            6739..6978
                     /gene="dltC"
                     /locus_tag="VBD85_000122"
     CDS             6739..6978
                     /gene="dltC"
                     /locus_tag="VBD85_000122"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO7132701.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            6971..8233
                     /gene="dltD"
                     /locus_tag="VBD85_000123"
     CDS             6971..8233
                     /gene="dltD"
                     /locus_tag="VBD85_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO7132702.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYLVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRPYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTGRPSDYK"
     gene            8408..8800
                     /locus_tag="VBD85_000124"
     CDS             8408..8800
                     /locus_tag="VBD85_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO7132703.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDKDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DKDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            8800..10092
                     /locus_tag="VBD85_000125"
     CDS             8800..10092
                     /locus_tag="VBD85_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7132704.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNQDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKIQ
                     AAESISHAYVELENQINKGVEAMLQQDSNLSGEFKQWH"
     gene            10083..10475
                     /locus_tag="VBD85_000126"
     CDS             10083..10475
                     /locus_tag="VBD85_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132705.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KITNMLQETYDAVEYDLRYSNDSSDMMTLNRILDSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            10485..10766
                     /locus_tag="VBD85_000127"
     CDS             10485..10766
                     /locus_tag="VBD85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132706.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSRVEEAIESNQQKYNHL"
     gene            10948..11730
                     /locus_tag="VBD85_000128"
     CDS             10948..11730
                     /locus_tag="VBD85_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO7132707.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVIKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            11717..12283
                     /gene="rnmV"
                     /locus_tag="VBD85_000129"
     CDS             11717..12283
                     /gene="rnmV"
                     /locus_tag="VBD85_000129"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO7132708.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEEDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDNFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGYEG"
     gene            12287..12736
                     /locus_tag="VBD85_000130"
     CDS             12287..12736
                     /locus_tag="VBD85_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7132709.1"
                     /translation="MYLICQRINMIEQNENPYFVKEYETGYLVLGDYQYFQGYCLFLS
                     KKHVTELHELPRDWRNQYLSEMADASEIVAKAFRADKMNIESLGNGDAHLHFHLFPRK
                     TGDLRNYGHNGKGPVWWYPFEKMYADSVRATGAEIEKLKEKLLDVLG"
     gene            12764..13636
                     /gene="rsmA"
                     /locus_tag="VBD85_000131"
     CDS             12764..13636
                     /gene="rsmA"
                     /locus_tag="VBD85_000131"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO7132710.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMMQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSVILKMVRRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVEI"
     gene            13640..14491
                     /locus_tag="VBD85_000132"
     CDS             13640..14491
                     /locus_tag="VBD85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7132711.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSGNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            14647..15519
                     /gene="rsgA"
                     /locus_tag="VBD85_000133"
     CDS             14647..15519
                     /gene="rsgA"
                     /locus_tag="VBD85_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO7132712.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIREHYQNIGYVFCYSQEELLPLLADKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISDSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            15526..16188
                     /locus_tag="VBD85_000134"
     CDS             15526..16188
                     /locus_tag="VBD85_000134"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7132713.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGTDIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            16181..16813
                     /locus_tag="VBD85_000135"
     CDS             16181..16813
                     /locus_tag="VBD85_000135"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO7132714.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASVEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDTDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            16815..18089
                     /locus_tag="VBD85_000136"
     CDS             16815..18089
                     /locus_tag="VBD85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO7132715.1"
                     /translation="MLVIILIIALASLTVTIISYQKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEAAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRALNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            18079..19020
                     /locus_tag="VBD85_000137"
     CDS             18079..19020
                     /locus_tag="VBD85_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO7132716.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYLELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            19117..19929
                     /gene="purR"
                     /locus_tag="VBD85_000138"
     CDS             19117..19929
                     /gene="purR"
                     /locus_tag="VBD85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO7132717.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            20148..20561
                     /gene="rpsL"
                     /locus_tag="VBD85_000139"
     CDS             20148..20561
                     /gene="rpsL"
                     /locus_tag="VBD85_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO7132718.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            20583..21053
                     /gene="rpsG"
                     /locus_tag="VBD85_000140"
     CDS             20583..21053
                     /gene="rpsG"
                     /locus_tag="VBD85_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO7132719.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            21208..23286
                     /gene="fusA"
                     /locus_tag="VBD85_000141"
     CDS             21208..23286
                     /gene="fusA"
                     /locus_tag="VBD85_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO7132720.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            23491..24501
                     /gene="gap"
                     /locus_tag="VBD85_000142"
     CDS             23491..24501
                     /gene="gap"
                     /locus_tag="VBD85_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO7132721.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            24710..25579
                     /locus_tag="VBD85_000143"
     CDS             24710..25579
                     /locus_tag="VBD85_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO7132722.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            25714..26910
                     /locus_tag="VBD85_000144"
     CDS             25714..26910
                     /locus_tag="VBD85_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO7132723.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            27173..27712
                     /locus_tag="VBD85_000145"
     CDS             27173..27712
                     /locus_tag="VBD85_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO7132724.1"
                     /translation="MKTLKKLLSNYKFDIKKFKLGMRTFKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            27792..28163
                     /locus_tag="VBD85_000146"
     CDS             27792..28163
                     /locus_tag="VBD85_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7132725.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            28197..29543
                     /gene="glnA"
                     /locus_tag="VBD85_000147"
     CDS             28197..29543
                     /gene="glnA"
                     /locus_tag="VBD85_000147"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO7132726.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            29691..30200
                     /locus_tag="VBD85_000148"
     CDS             29691..30200
                     /locus_tag="VBD85_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7132727.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASATSAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(30362..32041)
                     /locus_tag="VBD85_000149"
     CDS             complement(30362..32041)
                     /locus_tag="VBD85_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7132728.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKHLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPEYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(32095..32325)
                     /locus_tag="VBD85_000150"
     CDS             complement(32095..32325)
                     /locus_tag="VBD85_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO7132729.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            32504..33196
                     /gene="tsaB"
                     /locus_tag="VBD85_000151"
     CDS             32504..33196
                     /gene="tsaB"
                     /locus_tag="VBD85_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO7132730.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFVDQIKKVLPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLRNH
                     CETNTEEYIKRV"
     gene            33198..33605
                     /gene="rimI"
                     /locus_tag="VBD85_000152"
     CDS             33198..33605
                     /gene="rimI"
                     /locus_tag="VBD85_000152"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO7132731.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLGKRKNYYHDPIEDAIVMRKG"
     gene            33681..34691
                     /gene="tsaD"
                     /locus_tag="VBD85_000153"
     CDS             33681..34691
                     /gene="tsaD"
                     /locus_tag="VBD85_000153"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO7132732.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPVNHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKHPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            34810..35835
                     /locus_tag="VBD85_000154"
     CDS             34810..35835
                     /locus_tag="VBD85_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO7132733.1"
                     /translation="MLHFENDYNKGAHTELLNALIETNDQGLSGYRTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDDLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(36196..36465)
                     /gene="rpsN"
                     /locus_tag="VBD85_000155"
     CDS             complement(36196..36465)
                     /gene="rpsN"
                     /locus_tag="VBD85_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO7132734.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
     gene            36624..37406
                     /locus_tag="VBD85_000156"
     CDS             36624..37406
                     /locus_tag="VBD85_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO7132735.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            complement(37449..38621)
                     /locus_tag="VBD85_000157"
     CDS             complement(37449..38621)
                     /locus_tag="VBD85_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7132736.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLVV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELETHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLETLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            38756..39292
                     /locus_tag="VBD85_000158"
     CDS             38756..39292
                     /locus_tag="VBD85_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO7132737.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            39336..39923
                     /locus_tag="VBD85_000159"
     CDS             39336..39923
                     /locus_tag="VBD85_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO7132738.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSSVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIETVLTDFQSFVGDTPLIGYNGHKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            40050..40775
                     /locus_tag="VBD85_000160"
     CDS             40050..40775
                     /locus_tag="VBD85_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7132739.1"
                     /translation="MSEKYSTGDLAKEAGVTVRTVQYYDKRGILSPSELSEGGRRVYS
                     IADLEKLRQIIYLRDLDFSIDNIKNLFTEDNASQILELFLQVQIRELRLSIDSKKDKL
                     DKAVNLLKTVEKQDSKTLGYLSDIVLMEENKRKWGKLQKSIVLQVIMIIMVYITLIMA
                     AVSLNQRWLMWIAVTVFLIAMNGMVWYFKQQVVYLCPNCHQTFTPTYQAFNLAKHTPK
                     TRKVFCPHCHQKSHCIELAREER"
     gene            complement(40845..42035)
                     /locus_tag="VBD85_000161"
     CDS             complement(40845..42035)
                     /locus_tag="VBD85_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7132740.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGNNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIDGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAHPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIHETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(42196..42495)
                     /locus_tag="VBD85_000162"
     CDS             complement(42196..42495)
                     /locus_tag="VBD85_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO7132741.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            43407..43835
                     /locus_tag="VBD85_000163"
     CDS             43407..43835
                     /locus_tag="VBD85_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7132742.1"
                     /translation="MQDEEVMWAYDKSFTDQDCQYWLDWNMNSYRSDGFGLWKIIHKE
                     TSEVVGECGITMQTVEGENYPEIGYHLKKEHWNKGIATEVARAVRDYAFEELNLEELI
                     IISRDINLQSMNIAIKSGFTIKKRFLKNDRFPHFMFYLKR"
     gene            43845..44636
                     /locus_tag="VBD85_000164"
     CDS             43845..44636
                     /locus_tag="VBD85_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEO7132743.1"
                     /translation="MLPYYVVNAFTNKAFSGNPAGVVVLNEPIKEDLMQKIAKENKLS
                     ETAFILKEKDLYKIRWFTPEKEVDLCGHATLASTYVISRYLEDDLSHITFSSKSGLLE
                     VTISGEDITLFFTMVDVKQVPITAMMRESIEPTILEAYRTADEEHLVLYLENQESIEN
                     FKIDLEKISQLAPHGVTLTAQSSEKNIDYVLRYFAPNYGINEDPVTGSAQTRLAPIWT
                     KKLGKEQLVSKQLSERQGLMMIELEENLVKISGQANLYLKGNIDI"
     gene            44678..45208
                     /locus_tag="VBD85_000165"
     CDS             44678..45208
                     /locus_tag="VBD85_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEO7132744.1"
                     /translation="MRKREMKLELYNNKWPEKYQEIKEELTKLLSGLEIECHHFGSTS
                     IKGMASKPIIDVLIFVNNINMVDTYNNSMLNSGYVAKGENGILGRRYFVKYAKDKINH
                     LVHLHFYEKEDPKGLQELQFRDYLIENKTAFDRYLAVKVEASKKYKDMPEAYQNHKST
                     VIDAINKEIVKTKVSK"
     gene            complement(45741..46478)
                     /locus_tag="VBD85_000166"
     CDS             complement(45741..46478)
                     /locus_tag="VBD85_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7132745.1"
                     /translation="MPLNQFFILNNGVKIPSLGFGVFQIDDPLECEQSVLDALQVGYR
                     LIDTASGYLNEEAVGRAIKKSGIPRKEIFVVTKLWIQDASEEKAGPAIDRALKRLQLD
                     YINLYLIHQPMGDYYGACRALEKAYEERKALAIGVSNFSNGRVQDLILHNKIIPAVNQ
                     IQMNPFHPAKDQVEWLSKKGIHTMAWGPFAEAKYGIFQHPTLVEIAERYNKNCVSNNP
                     SLGSEKQSNSHFKNCAQSSYYGKYGLF"
     gene            complement(46484..46834)
                     /locus_tag="VBD85_000167"
     CDS             complement(46484..46834)
                     /locus_tag="VBD85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEO7132746.1"
                     /translation="MEGLSYELYPFNIYCKLIEPGGIQTDFWNRSLEITDGVKESPYS
                     TYAEKYLEDMTEPVSSNRSSPDLVAETIYIAATDNIKQWRYLVASEEFINYRKSMTDQ
                     EWIASMMKNYLGKD"
     gene            complement(46850..47161)
                     /locus_tag="VBD85_000168"
     CDS             complement(46850..47161)
                     /locus_tag="VBD85_000168"
                     /inference="COORDINATES: protein motif:HMM:NF012334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO7132747.1"
                     /translation="MKVFKLDVTDSFSIQKAFLEIKEEYKTIDCLLNNAGYALFGPLE
                     LSSEEQIRRAYDTLSLGVTFVTKQFIPLLRENGGGSIITISSVGGVLTLPLTATYHAA
                     K"
     gene            complement(47201..47302)
                     /locus_tag="VBD85_000169"
     CDS             complement(47201..47302)
                     /locus_tag="VBD85_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEO7132748.1"
                     /translation="MTKTIVITEASSGIGEATAKFFAKKGWQVAATM"
     gene            47422..47595
                     /locus_tag="VBD85_000170"
     CDS             47422..47595
                     /locus_tag="VBD85_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7132749.1"
                     /translation="MYIQQLETFVKVAEEGSFSKAAKKLYISTSAVAQQINLLENRYQ
                     FNTCRSVFIHGWS"
     gene            47606..47905
                     /locus_tag="VBD85_000171"
     CDS             47606..47905
                     /locus_tag="VBD85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132750.1"
                     /translation="MSKLAIEKGRSIASQKEIIKIGTADIFKPRLLMKFVNYFKEHIF
                     PYDFAFPHTSEAECDVVEAIYCDIAWKGHCQFYELFQTPLAVAVNSNHPLALKKR"
     gene            47938..48246
                     /locus_tag="VBD85_000172"
     CDS             47938..48246
                     /locus_tag="VBD85_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7132751.1"
                     /translation="MPQKNALTGMDQFRNALLSEFSQAKIIDVPVIGQETFMMCELEP
                     HVFITENVFADVHPNLITIPLESEFSLPYDLIYSNNPSSSTLGFIKTIADSKLTFSID
                     "
BASE COUNT        15889 a         7599 c         9497 g        15378 t
ORIGIN      
        1 agctgtctct gtaatatcta agacaattaa tcaatggatt aacgctgttt ttcctataca
       61 ttatattatt ttactaatca tttttatttc atgtgtgtta gcactttgtt atcgtctgtt
      121 tagacctagc tttattgaaa aaaatgactt gttaaaagaa atttctgaca ttaccatttt
      181 ctggctgatt attcgtttga tcggtttggc acttggtctg atgacggttt tacatattgg
      241 tcctgaaatg gtatggggga aagagacagg cggccttatc ctttttgatt tgattggtgg
      301 tttgtttacc attttccttg ctgcgggttt tattcttcca tttttgacag aatttggtct
      361 actggagttt gttggtgtct tcttaacccc cattatgcgt ccattctttc agctacctgg
      421 tcggtcagca gttaattgtg tagcctcatt tgtaggtgat ggcactattg gaattgcttt
      481 gaccgataag cagtatgttg agggttatta taccagtaga gaagctgcaa cgatttccac
      541 tactttttca gcagtatcaa tcactttttg tcttgttgtt ttgcagaatg ttggcttaac
      601 tgattatttt ggccattttt atctaacagt catcattagc agtgttgtct gtgcattcat
      661 tatcccttat atccctccct tatctcgtca atcaaatagt tactacacta aaccaaagaa
      721 agaaacatct gaggtaattc cggatggtta taacaagata gagtggggac ttaagcttgc
      781 catcgataaa tcaaaagcaa atggaaacat gaagactttt gtcaataatg cctctaagac
      841 agtacttagt ttatggtttg gcgttatgcc aacaattatg actgtcggaa caatagcttt
      901 gattatttca gtatcaacac ctatttttaa aattttaggc acaccattcc ttccgttcct
      961 tgaactttta gggattccag aagcagatat tgcgagtcaa accatgattg ttggtttttc
     1021 agatatggtt gttccttcga taatggcggc tgaaattcac agtgagatga caaggtttat
     1081 tgtcgcaaca gtttctattg tgcaattaat ttatatgtct gagactggtg cggtgatatt
     1141 aggttccaag atccctatca acattctaga attatttatc atttttatag agagaacaat
     1201 tatttcatta cctattattg ttttgatggc acatcttttc ttttagttgg agaaaataga
     1261 gaagcatttc ttgctaacaa atggaactat ttactgaaaa aagacaaatg aacttgaaag
     1321 tatttcttga caagaggcct aaaattgtta taaaatagta tggtatgttt agtaactgat
     1381 caaaatagcc ggctaaacga atacgaaaat ctatcaggag gtattcaacg tgaaacgtac
     1441 ttatcaacca agtaaaatcc gtcgccaacg taaacacgga ttccgtcacc gtatgtcaac
     1501 taaaaacgga cgtcgcgtgc ttgctagccg ccgtcgtaaa ggacgtaaag tgttatcagc
     1561 ttaatataga tagcaaaata atatcaagcc aacaggtact cgagtctggt ggcttttttg
     1621 gtaccttcat aacgataaag gtttttgtga gagtagtttg agtatattat agataaagtt
     1681 aatgaaaagt gagtttataa tgcggaaatt cgtagacaaa tagctcctat ttttgttaaa
     1741 atatgaggag atttcttatt gagaaatatc tttatagtga gtgatatttt attttttaga
     1801 cgcttgtatt attgaagtag tttagtgact taggaaatac ttttattaat atcgtttttt
     1861 caaggttatt tcaattttgc tagtttatac tataatatat aaggtaaggt gactagaaaa
     1921 tgagacttct tgtagttgag gatgaaaaaa gtatagcaga ggctattcag gctttattgg
     1981 cagataaagg ttattctgtt gatttagtat ttgatggaga tgatgggtta gaatacattt
     2041 tgacaggtct ctatgattta gtcttgttgg atattatgtt acccaaacgc tcaggattat
     2101 ctattttaaa aagagtgagg gaagctggtt tagaaacccc aataatcttt ttaactgcaa
     2161 aatcacagac ttatgataaa gttaatggtt tagatttagg ggcagatgat tatatcacaa
     2221 aaccctttga agcagatgaa ttgctagcta gaattaggtt gagaaccaga caatcttctc
     2281 tgatcagagc aaatcaattg cggcttggaa atataaggct taacacagat tcacatgagc
     2341 tagaatctaa agaaagtagt gtaaaattat ccaataaaga atttctcttg atggaagttt
     2401 ttatgcgaaa tgcaaagcag attattccta aaaaccagtt gatttctaag gtttggggac
     2461 cttcagacaa ttcagaatat aaccagttag aagtatttat ttcttttttg cgaaaaaaat
     2521 tgcgcttttt aaaagcagac attgaaataa taaccactaa gggctttgga tattccttgg
     2581 aggagatgac ataatgttta gtgatttacg caaaaaattt gtctttttga ccatgtcaat
     2641 ccttattgtc gttgttctct ttctctttgc agtgagtaat cgctacaatc aatattggga
     2701 tgaatatgat gcatatagga ttgttaaatt agttgctaaa aatgattatt taggaatacc
     2761 aggcgatgaa ccgattgctt tggtgactat agataaccaa aagatggtta agattcaaag
     2821 caataatact gatttgacaa atgatgttat cgaaaaatct agtttaaagc tccttgagca
     2881 aggaaagaag tcacgaaaat ggaagtcttt tatatattcc ataaaagaat ataaagataa
     2941 aacttacacc atagctatta tggatttggc tagttatgaa gtcccttatg caaggcgttt
     3001 tctcattctt gtttttacta tttttggatt ttgtttatta gcagctgtaa gtctttatct
     3061 ttctcgattt atcgtagggc ctgtagagac agaaatgact cgcgaaaaac agttcgtttc
     3121 agatgcgagt catgagctga agacccctat tgcagctata cgagccaacg ttcaggttct
     3181 ggaacagcaa attccaggaa atcgttatct ggatcatgtt gtttcggaga ctaagcgaat
     3241 ggagttttta attgaagatt tgcttaattt gtctagactt gatgaaaaac gctccaaggt
     3301 aaatttcaaa aagttaaact tatctgtctt atgtcaagaa gtacttctaa catatgaaag
     3361 tttggcatat gaagaagaaa aatgtttaaa tgatacaatt gaagatgatg tctggattgt
     3421 tggagaagag agtcaaatta agcaaatttt aataattctt ttggataatg ctattaggca
     3481 ttctttgtca aaatcagcaa tccagtttag tcttaaacag gctcgaagaa aagctattct
     3541 gacaatatca aatccaagtg ctatctattc caaagaggtt atggataatc tctttgagcg
     3601 tttttatcaa gctaaagatg atcatgcaga ttctctcagt tttggtctag gcttatctat
     3661 tgctaaggca atcgtggaac gccataaggg gagaattaga gcatatcaag aaaaagacca
     3721 attacgtctt gaagtccagt taccaattga tggattttgg accaatacca tgattaatta
     3781 aaggaagaat gatgaaacca tatttatctt ttattggtag aacgttatta tacttcggta
     3841 ttttattgtt actaatttac ttttttgcat accttggtcg cggacaaggc agttttattt
     3901 ataatgaatt ctaggagatg gtacaaatga tacatgatat gattaaaaca attgagcatt
     3961 ttgctgagac acaagctgat tttccagtgt atgatatttt aggggaagtc catacttatg
     4021 gacaacttaa agtagactct gactctctag ctgctcatat tgatagccta ggccttgttg
     4081 aaaaatcacc tgtcttagta ttcggtggtc aagaatatga aatgttggcg acatttgttg
     4141 ctttaacaaa gtcagggcat gcttatatac cggttgacca acactctgct ttggatagaa
     4201 tacaggctat tatgacagtt gctcaaccaa gccttatcat ttcaattggt gaatttcctc
     4261 ttgaagttga taatgtccca atcctagacg tttctcaagt ttcagctatt tttgaagaaa
     4321 agactcctta tgaggtaaca cattctgtta aaggtgatga taattactat attattttca
     4381 cttcagggac tactggttta ccaaaaggtg tgcaaatttc acatgacaat ttattgagct
     4441 ttacaaattg gatgatttct gatgatgagt tttcagttcc tgaaagaccg caaatgttgg
     4501 ctcaaccgcc atattcattt gacttatcag ttatgtattg ggcaccaaca ctagctatgg
     4561 gaggcaccct gtttgcccta ccaaaaacag tagttaatga tttcaaaaaa ctattcgcta
     4621 ccattaatga attgccaata caggtttgga cttcgacacc atcatttgct gatatggcgc
     4681 tactatctaa cgatttcaat tcagagacct tgccacagtt aacacatttt tattttgatg
     4741 gggaagagtt aactgtcaag actgcacaaa aacttcgtca acgttttcca aaagctcgta
     4801 tcgttaatgc atatgggcca acagaagcaa cagttgctct atccgcagta gcaattactg
     4861 atgaaatgtt agaaacatgc aaacgccttc caattggtta cactaaagat gactcaccaa
     4921 cgtatgtgat tgatgaagaa ggtcataaat taccaaacgg agagcaaggt gaaatcatta
     4981 ttgctggacc agcagtatca aaaggctatc ttaacaatcc agaaaagaca gctgaggcat
     5041 ttttccaatt cgaaggtcta cctgcttacc ataccggtga cttaggaagt atgaccgatg
     5101 aaggtcttct gctttacggt gggcgtatgg atttccaaat taaatttaac ggctatcgta
     5161 ttgaattaga agatgtttct caaaacttaa acaaatcgca gtatgtaaaa tcagcagtag
     5221 cagtgccacg ttataacaag gatcataaag ttcaaaactt attagcctat attgtcttaa
     5281 aagaaggtgt aagagatgat tttgaacgtg atttggattt gacaaaagca attaaggaag
     5341 acttaaagga cattatgatg gattacatga tgccatctaa atttatctat cgagaagatt
     5401 tacctttgac accaaatggg aaaattgata tcaaaggtct tatgagcgag gtaaacaagt
     5461 gatacaggca tttttagaaa aacttcctca cttggatgtt tatgggaatc cacaatattt
     5521 cttctacctt atcttagctg ttctaccaat ttacatcggc ttattcttta agaagcgttt
     5581 tgccttatat gagattattt ttagtctaag ttttattgta atgatgttga ctggtagtac
     5641 ttttaatcaa ttgaagtcac tattggcata cgttgtcgga caatctctgc tagtttttat
     5701 ctataaagct taccggaaac gatttaatca tactttggtc ttttatgtaa cggtttgttt
     5761 atctattttt ccgctatttt tggtaaaatt aattccagct atatctgagg atgggcatca
     5821 gtcacttttt gggtttctag gaatttctta ccttactttt agagctgttg ctatgattat
     5881 tgaaatgaga gacggtgttt tgaaagaatt tactttatgg gaattcttaa gatttttact
     5941 cttctttcca actttctcaa gtggaccaat tgatcgtttt aaacgattca atgaggatta
     6001 cattaatatc ccagatcgaa acgaactcct agatatgtta ggtcaagcga ttcattattt
     6061 gatgttaggt tttctctata agtttatttt agcctatatt tttggaagtc tgattatgcc
     6121 tcctctaaaa gaattagcgc tagaacaggg tggtgtgttt aattggccaa cacttggggt
     6181 tatgtatgcc tttggttttg atttgttctt tgattttgca ggttacacaa tgtttgcgtt
     6241 ggctatttct aacctaatgg ggattaagtc tccgattaac tttgacaaac ctttcaaatc
     6301 acgcgaccta aaagaatttt ggaatagatg gcatatgagc ctttctttct ggtttagaga
     6361 ctttgttttc atgaggcttg ttaagctttt agttaaaaat aaagttttta aaaaccgtaa
     6421 tgttacttca agtgtagctt atattatcaa tatgcttctt atgggattct ggcatgggtt
     6481 aacttggtac tatatagcct atggtctctt tcatgggatt ggcctagtta ttaatgacgc
     6541 ttgggtacgt aagaagaaaa atattaataa agaaagaaga ttggctaaaa aaccactttt
     6601 accagaaaac aaatggactt atgctttggg tgtcttcatc acctttaatg tagttatgtt
     6661 ttctttcttg attttttcag gatttttaga tcttttgtgg ttcccacaac cgcataacaa
     6721 ataataagga gtattataat ggatattaaa tcagaagtat tagcaattat tgatgaccta
     6781 tttatggagg atgtttcttc tatgatggat gaagacttat ttgacgcagg agttctagac
     6841 agcatgggta ctgttgaact aattgttgag ttggaaagcc attttaatat tgatattccg
     6901 attgcggaat ttggtcgtaa tgactggaac acagctaata aaattgttgc tggagtaacg
     6961 gagctctgca atgcttaaac gtctaggtaa agtatttgga cctctagttt gtgctttact
     7021 attgttggta ggattatatc ttgtttttcc tgtttctcag cctcatcatt taggtaagga
     7081 aaaaaacagt gcagtagcgt tgacgaaggc aggttttaaa agcagagttc aaaaagttag
     7141 agctttcagt gatcctaaag ccaattttgt ccctttcttt ggttcaagtg agtggctaag
     7201 atttgatgca atgcatccat cagttttagc agaggcttac aatagacctt atatcccata
     7261 tcttttaggt caaaaagggg cggcttctct gacacaatac tatggcattc aacagattaa
     7321 aggacaaatc aaaaataaaa aagctatcta tgttatttct ccgcaatggt ttgttcgcaa
     7381 gggagccaac aaaggtgctt ttcaaaacta tttcagcaac gatcaaacca ttcgattttt
     7441 gcaaaatcaa acagggacaa cctacgatag gtatgctgct cgtcgattgt taaaattata
     7501 tcctgaagct tctatgtcag atttgataga aaaagttgca gatggccaaa aactatcaaa
     7561 taaagacaaa caaagactaa agtttaatga ttgggtattt gagaagacag atgctatttt
     7621 tagctatcta ccactaggaa aaacttataa tcaggcaata atgcctcatg ttggtaaatt
     7681 accgaaagca ttctcatata atcatttatc gcgtattgca tcacaagatg ctaaagtagc
     7741 aacgagatca aatcaatttg gtattgatga tcgcttttac caaacaagaa ttaaaaagca
     7801 cttaaaaaaa ttgaaagggt cacaacgaca tttcaattat actaagtcac cagaatttaa
     7861 tgatttacag ttggttctta atgaattctc aaaacaaaat acagatgtcc tttttgtcat
     7921 accaccagta aataaaaagt ggacagacta cacagggctt gatcaaaaaa tgtatcaaaa
     7981 atctgtagaa aaaataaaac accaacttca aagtcaaggt ttcaatcata tcgctgacct
     8041 ttctcgagat ggaggtaagc catactttat gcaagataca atccatttag gttggaatgg
     8101 ttggttagag ctagataagc atatcaatcc atttttaaca gaggaaaaca gcaagccgaa
     8161 ttatcacatt aataataaat ttttgaagaa atcttgggca aaatatacag gacgtccaag
     8221 tgattacaag taaatagtag agtcagatga tttatagcat tagagctatt aatcatcttt
     8281 tttaatatct ttgtatttgg taatcctaag ataactaatc catgttttat agtttttcgt
     8341 ttataatgag aataaattta tgataaacta gatatcaaat gagtgttata tgataaaagg
     8401 aggagagatg aagtcaataa aattacttag ttttattgta ataatccttt tagggcttgg
     8461 tttcactgta ggaggtttaa aagtgactga aaacaataaa caacaagaat tatgggaaat
     8521 agctaatagc aaggacgcta aaaatgctta tcaaaagtgg atttatgcag aagataaaga
     8581 cgctttcaaa gagaatgctg ttattaagtc gtatgatatt gacaaagaaa gtatcaaaaa
     8641 aaatcctatg ggagggattt cggtaaggtt aatcattaat aaagatccta acttatatat
     8701 tacttgtaat ttggataagg ataatcaagg gcatttggtc agtcaaagta gccatcaatc
     8761 tccccagtta actcatttat tggaaagtag aggtcattaa tggaaaaagt cttgactgat
     8821 ttagataggg taaatatagc gaagcaagag tatgaattag gtagccaact tgatacacta
     8881 gtcaaaataa tgagccaaga taaggtactc cccatcggta aagttgctca cgttcaagat
     8941 ggaggcaaag aaactgggga acaaatttat accatcactc caaatggaac tttagacaag
     9001 ccagaggatg ttaaggaagt tacggtttta tttaaggggt caacagcacc gtttggagga
     9061 gatgattgga aaacagactg gtttaaaaat gatattccaa tagctagtaa actattatta
     9121 aagaaatttg gttcacaaag tgtatctcat aaacaaggta caaagcagtt agaacaatca
     9181 gctcatctgc taaaagaagt tatgaataaa tatcctaatg caaaaatttc ggtctacgga
     9241 cactcgttag ggtcaatgaa tgcacagtat gctattgcag aattggatga taatcaaatt
     9301 taccgagttc aaggagcttg gatatataac ggtccaaacc tttatagtat tttgacagat
     9361 aaacaaaaag aacaggttga taaaataaaa ggggtagtca ataattacgt tgattccaag
     9421 gataaaattg gaataggtta tcctaaagaa ggaagcgata atgctgtcgg aattgttaaa
     9481 atagttgaca gtaaagctgt ttcaggaggt agctgggttg ctaattttac caatcaacac
     9541 atttggggag gctaccagta caatcaagat ggttctttaa aattagcaga agataaattt
     9601 gcttacaaag aaaactattc agtagttcat acgaatgtga atcaaagaat gcatgctatt
     9661 aaagcgaaac ttagtagctt taaaagtacg ggaaaagaag ttagtaagaa tcaagctatt
     9721 ttcctagata gtcaacaagc aaaagtgatt tctagtggtt tggtaacgat atcaaaagaa
     9781 gcagttgaag aagtaaaaaa agttgagcaa aaagcagttg ctaaagcaga agagctttat
     9841 caatctttaa aagtagctcc tacaggagtt actgaactaa gtcctcaaga attggaagaa
     9901 gcgtatgctc agtcaggtgt tagctatgcg agcattgtcg gagaagctca aacctttttc
     9961 tcaaataaaa ttcaagcggc agaaagcatt tctcacgctt acgttgaatt agaaaaccaa
    10021 attaataaag gggttgaagc tatgttgcaa caagatagta acttgtcagg ggagtttaaa
    10081 caatggcact gattgatatc attgaaaaac aattagctga tactcagaga aaaatatcag
    10141 atttagacga tgcctatcac catagttgtt gccagtttga agaaaaatta gacgatctct
    10201 cagtaagaaa aaataagatt actaatatgc ttcaagaaac ttacgacgct gttgaatatg
    10261 atttgagata tagtaatgat agctcagata tgatgacgct caatcgtatt ttagattctt
    10321 atcatgacga cttagagcaa gcttaccata aagagtacta tgccttgtcc gctcaagaag
    10381 aagaatatag agctaactat attcggcaac gttcagaaca tgaactaacc tttgaagagt
    10441 tgcaaaggga aaagaaaaga gaattaatga agtaggagaa attattgagt ttaatgtggg
    10501 atgtcctgac tggtaaggag tcagtaagga ctttaaaaaa acaattggat aaagaattga
    10561 tacatagcgt gtacgcgcca cgcaagagat ctaagtttca cgatattatt acgtcgcatc
    10621 ataaagaaat agacgaagta gccatcaaca caaagacagc tattcataaa gttgctaatc
    10681 aattggacga ggcaataaaa ggagaatttc gttcaagggt tgaggaggct attgaaagca
    10741 atcagcaaaa atataatcat ttatagtcta gtttctctac cattagctag acacttgtag
    10801 aggattttta cagcaagtat tcaaaagtca gagcaaaaat gttttgactt ttttgattta
    10861 ctctttctga tcagaatgct tcagtcttat gttataataa taaagattaa tgcttgtagc
    10921 tacgataagc aaagaaagaa cagtgatatg ataaaaatat ttgataccca tacccacctt
    10981 aacgtggaaa attttgaagg aaagatagac gaggaaatta atttagctag cgaactcggt
    11041 gttataaaaa tgaatgttgt cggctttgac caagatacca tttcaaaatc tttggaatta
    11101 tcttcccaat atgcacaggt atatagcaca ataggatggc atccaactga agctggttct
    11161 tatgatgata atattgagtc aatgattatc agtcatttag aaaatcccaa ggttattgca
    11221 cttggtgaaa ttggtctgga ttattattgg atggaggatc ccaaagatat tcagatcgag
    11281 gtgtttaagc gtcaaattga actttcaaaa gagtataacc ttccttttgt cgttcataca
    11341 cgtgatgcgt tagaagacac ttatgaagtc attaaggaat ctggagttgg gccttttggt
    11401 ggtataatgc actctttttc tggttctttg gaaatggctc agaaatttat tgatttaggg
    11461 atgatgattt ccttttctgg tgttgtgacc tttaagaagg cattagatgt acaagaagct
    11521 gcacgagagt tgccattaga taagatatta gtggaaacag atgctcctta tttggctcct
    11581 gtacctaaaa gaggtcgaga aaacaaaacc gcttatacga gatatgtcgt tgaaaaaata
    11641 gcagagctac gaggcataac tgtggaagag gtagcggagg ctacatacca aaatgcagta
    11701 aggatattta gacttgatga aaaaaattga tattcaagaa gtgatcgttg tcgaagggaa
    11761 agatgatact gctaatctgc gacgctttta taatgttgat acctatgaaa ctagaggttc
    11821 agcaattgat gaagaggatt tagagcgtat cgaacgtttg cacaatttgc ggggagttat
    11881 cgtctttaca gacccagatt ataatggcga gcgtatccgt aaaatcatca tgaatgccat
    11941 tccgacagtc cgccatgcct tcctcaatcg agacgaggca aagccaggct caaagaccaa
    12001 gggacgatct ctgggtgtcg agcatgccag ctttgaggat ttgcagaagg cactctcaaa
    12061 agtgacccag catttcgatg atgaggataa ctttgatatc acccaagcag acctcattcg
    12121 ctgggggttc attaccgcta gtgatagccg taaacgccgt gagtatcttg gaaatcaact
    12181 ccgtatcggc tactcaaatg gtaaacaact cctcaaacgc ctcagactct tcggagtgac
    12241 caaggcggaa gttgaagagt gtatggaggg gtatgaggga taaaacatgt atcttatttg
    12301 tcaacgtatt aatatgattg aacaaaatga gaatccttat tttgtgaagg agtacgaaac
    12361 gggttatctt gttttgggag actatcaata ttttcaaggt tattgccttt ttctttctaa
    12421 aaaacacgta acagagttac atgaattgcc tagagattgg cgaaatcagt atctttccga
    12481 aatggcagat gcttctgaaa tagttgctaa ggcttttaga gcagataaga tgaatattga
    12541 gagtttggga aatggagacg ctcatctaca ttttcatctt tttccaagaa aaacaggtga
    12601 tttaaggaac tatgggcata atggaaaagg tccagtttgg tggtatcctt ttgagaagat
    12661 gtatgcagat agtgtcagag caactggtgc agaaatagaa aaattaaaag agaaattgtt
    12721 agatgtctta ggatagactt gagacgataa ggaaataaaa aacatgagaa tcgcagataa
    12781 aaccgttacc cgcgctattt tggagcgtca tgggtttaca tttaagaaat cattcggcca
    12841 gaactttttg accgatacga atattttaca gaagattgtt gacacggcag agattgacaa
    12901 gggtgtcaac gtgattgaga ttggtcctgg tatcggggct ttgacagagt ttttggctga
    12961 gaatgcagct gaggtgatgg cttttgagat agatgatcgt ctcattccta tccttgctga
    13021 tacgcttgcg cgttttgaca atgtccaagt ggtcaatcag gatatcctca aggctgattt
    13081 gcagacgcaa attcaagcct tcaagaatcc tgatctgcct atcaaggtgg tagcaaacct
    13141 gccttactac atcacaacac caatccttat gcacttgatt gagagcaaga ttccttttgc
    13201 ggagtttgtg gtcatgatgc aaaaagaagt ggcagatcgt atttcagcta tgccaaatac
    13261 taaagcatac ggttctttgt caatcgcagt gcagtattat atgactgcca aagtatcatt
    13321 tatcgttccg agaactgttt ttgttcctgc tccaaatgta gattcagtta ttttgaaaat
    13381 ggtccgtaga gatcaaccag tagtgagtgt tcaagatgag gatttctttt tcagagtgtc
    13441 aaaagtagcc ttcgtacatc gtcgtaaaac actttggaat aatctaacga gtcattttgg
    13501 aaaatcagaa gatactaaag caaaattgga aaaagcatta gaaatagcaa aaattaagcc
    13561 atctattcgc ggagaagcct tatctatccc tgattttgca agtttagcag atgccttgaa
    13621 agaggttgag atataaacta tgttggttga caagaagtgg cgttttgaag atagtgcaag
    13681 ttatttcgct tgtcctaaat gtcaaaatcc cttgataaaa gaaagtaaca gcctcaaatg
    13741 ctctggtaat cattgttttg atttatccaa atttggatat gttaatcttc ttggaggaaa
    13801 gaaggtagat gaacattatg ataaaaaatc atttgaaaat cggcagcttg tcttagaaaa
    13861 cgggtattat aatcatattt tagaagctat ttcaaaggtt ttggaaaata attctcagtt
    13921 tcattcggtt ttagatattg gttgtggaga gggcttttat tctcgtcaat tagttaacaa
    13981 gcatgagaag actttcttgg cttttgatat ttctaaagat tcgattcaat tagctgctaa
    14041 aagtgatcag tctaggcttg tgaaatggtt tgtttctgat ttagctaacc ttcctattca
    14101 agactcatca attgatatca ttttagacat tttttctcct gcaaattata aagaatttag
    14161 gcgggtgctg agtgatgatg gtatccttgt aaaggttgtt cctgtagctg aacatgttca
    14221 agaattgcga gaaaaagcta gtcaatactt gaaacagaaa gattactcta atcaaaaaat
    14281 tctagatcac tttagagaaa attttgaaat tatttctgag cagaaagttg ttcaaagcta
    14341 taactgttct caacaagaac gtcaagcttt tattgatatg acaccgttgt tattcagcgt
    14401 agataaaacg actattgatt gggcgagtat atcagaaatt acagtaggtg ccttaatagt
    14461 aattggaaaa aagcgcagtg tatctaaata agatcactgc ttttatgttg ggaattcata
    14521 aaattgaggt tatttgagag aaatcgaaca cctgcaaata gtttctattt agcttatctc
    14581 tgagagtaga gcttatttct cattttatga tataatagtg gtattctaaa caattaagga
    14641 gaacaattgc aaggtagaat tgtcaagtcg ctagcaggtt tttattatgt ggaatctgac
    14701 ggtgttgttt atcagacacg tgcgcgtgga aattttagaa aaaaaggtca aattccctac
    14761 gttggggatt gggttgaatt ttcttcgcaa gatcaatcag aaggatatat tctatcaatt
    14821 gaagagcgta aaaatagcct cgtacgacca cctattgtta atattgatca agctgtcgtt
    14881 atcatgtctg ctaaagaacc tgattttaac gctaacttat tagaccggtt cttagtactt
    14941 ctagaatata agatgattca gcctatcatt tatatttcca agttggattt gttagacgat
    15001 ttggttgtta ttgatgatat tcgcgaacat tatcagaata taggctatgt tttctgttat
    15061 tctcaagagg aactcttgcc tctgttagca gataaagtga ctgtttttat ggggcagaca
    15121 ggtgtcggaa aatcaacgct tctcaacaaa attgctcctg agttgaaact tgaaactggt
    15181 gaaatttcgg atagtttagg ccgtggccga cacaccacaa gagcagttag tttctataat
    15241 gttcataaag gaaaaattgc agacactcct ggtttttctt ctttggatta tgaagtggac
    15301 aatgcagagg acttaaacga atcctttcca gagcttcgta gacttagtca tttttgtaaa
    15361 ttccgctcgt gtacgcatac acatgagcct aaatgtgctg taaaagaagc cttgactcaa
    15421 ggtcagttat ggcaagtccg ctatgataac tacctccaat ttttaagtga aattgagaac
    15481 cgccgtgaaa catataagaa agttattaaa agaaagtagg tttctatgtc aaccaataaa
    15541 attgctccat cgatattagc agcagactat gctaattttg ctaacgaatt gaaacgtatt
    15601 gaagagacaa cagcagaata cgttcatatt gatatcatgg acggtcaatt tgtccctaat
    15661 attagttttg gtgcggatgt tgtatccagc atgcgtaaac acagtaaact tgtttttgat
    15721 tgtcatctga tggttgttga tccagaacgt tatattgagg cttttgctca agctggtact
    15781 gatatcatga ccattcatgt tgaagcaacg aaacatatac atggtgcgct tcaaaagata
    15841 aaagaggcag gaatgaaagc aggtgtggtt attaacccag gaacacctgt agaatctctt
    15901 attcctattt tagatttggt tgaccagatt ttaattatga ctgttaatcc tggctttggt
    15961 ggacaagcct ttattccaga aatgatgtca aaagtaaaga cagttgcagc atggcgcaaa
    16021 gagtacggtc atcattatga tattgaagtt gatggcggta tagataatac tacaattaag
    16081 gcagcagcag aggctggtgc aaatgtcttt gttgcaggtt cttatctctt taaagcaagt
    16141 gatttacctg cacaagtaga aaccttaagg gtggcgctcg atgactaaaa ttgctttatt
    16201 tgctggcggt gacttaactt attttgaata tgattttgac tattttgtcg gaattgaccg
    16261 tggaagccta tttttattaa aaaatgggct ttctttggac atggcagtgg gtgattttga
    16321 ttctataaca gaagatgaac tgctttatat caaacactat tgtagcaata tcgtttctgc
    16381 tagtgtagaa aaaaatgata cagatacaga attagcttta aaaactattt tcaaagaatt
    16441 tccagaagct caagtgaccg tatttggagc atttggtggc aggattgacc atatgatgtc
    16501 taatatcttt ttaccaagtg acacagattt agaaccattt atgtcacaaa tcagactcaa
    16561 agatgaacaa aacatagtga cttatctacc atccgggaaa aatcaagtct caaggattga
    16621 agggatgtcc tatgtctcgt tcatgcctga gagtgagtct actttgcaga tttctggggc
    16681 taaatatgaa ttgaacaaaa gtaattattt taagaaaaaa atgtattcca gtaatgaatt
    16741 tatgacttcg ccaattgaag tagagctcaa agatggttac ttaatcatta tttatagtaa
    16801 ggatagaggt taatatgctt gtcatcattt tgatcattgc actagctagt ctgacagtga
    16861 cgataatttc ttaccaaaaa atgacggaat taacaaagtc cgttgaaaaa caacttgaag
    16921 ataatgctga taatctatca gaccaactga cataccagat agaagcggcg caaaaagatc
    16981 aaattctaac gttgactaat cagctaaacc gtatgcaaca ggaaatttat cagttgctta
    17041 ccgatatgcg tacagaatta aatcagcatt taacggagag tcgagatcga tcagacaaac
    17101 gcttggaatt gattaactcc aatttatctc agtcagttca gaaaatgcaa gattccaatg
    17161 aaaaacgctt ggatcaaatg cgccaaactg ttgaggaaaa gctagaaaaa acgctacaaa
    17221 cgcgtttgca aacttctttt gaaactgtat cgcgtcaact agagagtgtc aatcaaggtc
    17281 ttggggagat gaaaactgtt gcccaagatg ttggtacact gaacaaagtc ttgtcaaata
    17341 ctaagacaag gggaatatta ggggagttac aactcggcca aattatagaa gatattatga
    17401 cagttagtca atatgagaga gaatttccta ctgtgtctgg ctcttctgag cgtgttgaat
    17461 atgctattaa attacctgga aatggtcagg gagattatat ctatttgcct attgactcta
    17521 agtttcctct agaagattat taccgattgg aagatgctta tgaattgggt gacaaggttc
    17581 aaatagaact ctatcgtaaa tctttactgg caagtattcg taaatttgca aaagatataa
    17641 acaataagta cttaaatcct cctgaaacga caaattttgg tatcatgttc ttaccaactg
    17701 aagggctcta ttctgaagtg gtaagaaatg caacattctt tgatagtcta agacgtgacg
    17761 aaaatattgt agtagctgga ccgtcaacct tatctgcttt actaaattca ttatctgttg
    17821 gctttaaaac cttaaatatt caaaagaatg ctaatgatat tagtaaaatt ttagggaatg
    17881 tcaaggttga atttggaaaa tttggtggca tgttatcaaa agctcaaaaa caattaaata
    17941 cagcaagtaa aagtattgat agtttactca caacgcggac aaacgctatt atcagagcct
    18001 taaatacagt tgaagaacat caagatcaag caaccactag cttgttaaac cttcctataa
    18061 cagaagaaga ggaaattaat gaaaattaac caaatgaaaa aagacgagct ttttgagggg
    18121 ttttacctta taaagaaagc agaagttcgt aaaacgcgtg caggtaaaga ctttattgcc
    18181 tttacctttc aagatgatac tggcgaaatt tcaggaaata tgtgggatgc acaaacctat
    18241 aatgtagaag agtttgtagc cggtaaaatt gttcatatga aagggcgtcg tgaagtttat
    18301 aatggaactc cccaagtcaa ccaaattaca ttaagaaaca tcaaagatgg tgaacctaat
    18361 gatcctagag attttaaaga aaaacctcca atcaatgttg acaacgtgag agagtacatg
    18421 gaacaaatgc tctttaaaat tgaaaatgca acctggcagc gtgtggtaag agcactttat
    18481 cgtaaataca ataaggaatt ttttacctat ccagccgcca aaacaaacca ccacgccttt
    18541 gaatcaggat tggcatatca cacggcaacg atggttcgtt tggcagatag tatcggagat
    18601 atctatctag aacttaataa aagtttgatg tttgctggta ttatgctaca tgatttagcc
    18661 aaggtcattg agttatcggg tcctgataat acagaatata ctattcgagg taatcttatc
    18721 ggtcatattt cacttattga tgaggaatta acaaaaatac tagcagagct taatattgat
    18781 gatacaaaag aagaggttac agtgttacgc catgttattc tgagtcatca tggacaatta
    18841 gaatatggaa gcccggttcg tcctagaatc atggaggcag aaattatcca tatgatcgat
    18901 aacattgatg caaatatgat gatgatgaca acagctttaa accgtgtcaa cgaaggtgaa
    18961 atgacaaatc gcatttttgc catggataac cgttcttttt ataaaccgaa cattaaataa
    19021 tttaggtaga ataattaaga cctaaaataa aatgttcgtt tttagcattt gttatgttat
    19081 actatatgta aagaatgcta tgaataagga gaaaatatga aattaagacg tagcgaacgc
    19141 atggttgtta tttcaaatta cctaataaat aatccctata cattaactag tttaaataca
    19201 tttgctagta aatacggtgc cgcgaaatct tcgatttcag aagatattgc tattatcaaa
    19261 aaagcttttg aacaagctca aatcggagat attaaaactg tgactggtgc aagtggaggt
    19321 gttatcttta caccaactat tgctgaagca gaagcgaaag aaatcgttga agaattaaga
    19381 cagcgtttat ctgaaaacga tcgtattctt ccgggaggtt acatttatct ttcagatcta
    19441 ctcagtacac caaaaatgtt acaaagtatt ggtcgcatta tagcgaacgc ttacagaggt
    19501 caaaaaattg atgctgtcat gacggttgca acaaaaggtg ttcctttagc aaacgcagta
    19561 gcaaatgttt tagatgttcc ttttgttatt gtgagacgtg atttgaagat tacggaaggt
    19621 tctactgtgt cagtaaacta cgctagtggt tcaagtggcc gtattgaaaa aatgtttctt
    19681 tctaagcgta gcttaaaacc taacagtcga gttttaatag ttgatgattt cttgaaaggt
    19741 ggaggtactg tttcggggat gattagttta ctctcagaat tcgatagcac attagtaggt
    19801 gtcgctgtat ttgcagaaaa tgctcaggaa caacgtgaga agatggccta taaatctctt
    19861 ctgagagtat ctgaaattga tgtaaaaaat aatagagtct ctgtagaagc ggggaatata
    19921 tttgattaaa gagtcaaggt gacatccttg attctttttg cttgtaaaag taataaaagc
    19981 ttgacacatc atgtgtaaaa tgatactatg ataaacggta cttttacttt tggtctctca
    20041 aaagtgtaca gagacgtgct gacaattgtt gcaaaagtac acgcaggtat aggctgtcac
    20101 caagtgctat atcaaccaaa aataaaaaac ttacaggaga aatgtagatg cctacaatta
    20161 accagttggt acgtaaacca cgtaaatcta aagttgaaaa atcagattca cctgctttga
    20221 atattggtta caacagtcac agaaaagttc acactaaatt atctgcacca caaaaacgtg
    20281 gtgttgcaac tcgtgttgga actatgacac ctaaaaaacc taactctgcc cttcgtaaat
    20341 ttgctcgtgt acgtttgagc aaccttattg aagttactgc ttacattcca ggtatcggac
    20401 acaacttgca agaacacagt gttgtgctta tccgtggtgg acgtgtaaaa gaccttccag
    20461 gggtacgtta ccatatcgtt cgtggtgcac ttgatactgc aggtgtagct gatcgtaaac
    20521 aaggccgttc taaatacggt gctaaacgtc ctaaagggta attagaaggg ggataagaga
    20581 aaatgagtcg taaaaatcaa gcgcctaaac gcgaagtatt accagatcca ttgtataact
    20641 caaaaatcgt tacacgtctt atcaaccgtg taatgcttga tggtaaacgt ggtactgctg
    20701 caactatcgt atatgatgct tttgaagcaa ttaaagaagc tactggaaca gatgctcttg
    20761 aagtattcga aacagctatg gaaaacatca tgcctgtact tgaggtacgc gcacgccgtg
    20821 ttggtggatc aaactaccaa gttcctgtcg aagttcgtcc agaacgtcgt acaactttag
    20881 gacttcgttg gttggttaac gcatcacgtg ctcgtggtga acacactatg aaagatcgtc
    20941 ttgcaaaaga aatcatggat gctgcaaaca acacaggagc atcagttaag aaacgtgaag
    21001 atactcacaa aatggctgaa gctaaccgtg cctttgcaca cttccgttgg taagattact
    21061 ctctttttga gacttatcat agctactaaa caaattgttt ggtagatagc aatgttagca
    21121 attagaacgg gtaggtcctg cctatccgtt ttttataaat agtgttataa taaagaagta
    21181 aataatttaa aatataggag aaaccaaatg gctcgcgaat tttcacttga aaaaactcgt
    21241 aatatcggta tcatggctca cgttgatgct ggtaaaacaa caacaactga gcgtattctt
    21301 tactatactg gtaaaatcca caaaatcggt gaaacacacg aaggtgcttc acaaatggac
    21361 tggatggaac aagaacaaga acgtggtatc acaattacat cagctgcaac aacagcacaa
    21421 tgggatggtc accgtgttaa catcatcgat acaccaggac acgtggactt cacaattgaa
    21481 gttcaacgtt cacttcgtgt acttgatggt gcggtaacag ttcttgatgc tcaatcaggt
    21541 gtagaacctc aaactgaaac agtttggcgt caagcaacag aatacggagt tccacgtatc
    21601 gtttttgcta ataaaatgga taaaatcggt gctgacttcc tctattcagt acaatcactt
    21661 catgaccgtc ttcaagcgaa tgcacaccca attcaattgc caattggttc agaagacgat
    21721 ttccgtggta tcattgactt aatcaaaatg aaagctgaaa tctatactaa tgatcttggt
    21781 acagatatcc ttgaagaaga cattccagca gagtatgttg atcaagcaaa tgaataccgt
    21841 gaaaaattag tagaagcagt tgctgatact gatgaagatt tgatgatgaa ataccttgaa
    21901 ggtgaagaaa tcactaacga agagttgatg gctgctattc gtaaagcaac tatcaacgtt
    21961 gaattctatc ctgtattgtg tggttcagcc ttcaagaata aaggtgttca attgatgctt
    22021 gatgctgtta ttgattacct tccaagccca cttgatattc ctgcaatcaa aggtattaac
    22081 cctgatactg acgaagaaga aacacgccct gcttcagatg aagagccatt tgcagcgctt
    22141 gccttcaaga tcatgacaga tcctttcgtg ggtcgcttaa cattcttccg tgtgtactca
    22201 ggtgttctta attcaggttc atacgtgttg aatacatcta aaggtaaacg tgaacgtatc
    22261 ggacgtatct tacaaatgca cgcaaatagt cgtcaagaaa ttgaaactgt ttatgctggt
    22321 gatatcgcag cggctgttgg tttgaaagat acaactacag gtgattcatt gactgatgaa
    22381 aaatcaaaag ttattcttga atcaattgaa gttccagaac cagttatcca attgatggtt
    22441 gagccaaaat ctaaagctga ccaagataaa atgggtatcg cccttcaaaa acttgctgaa
    22501 gaagacccaa cgttccgcgt tgaaactaac gttgaaactg gtgaaacagt tatctcagga
    22561 atgggtgaac ttcaccttga tgtccttgtt gaccgtatga aacgtgaatt caaagttgaa
    22621 gcaaacgttg gggcacccca agtatcatac cgcgaaacat tccgcgcttc tacacaagca
    22681 cgtggattct tcaaacgcca atctggtggt aaaggtcaat ttggtgatgt ttggattgaa
    22741 tttacaccaa acgaagaagg taaaggattc gaattcgaaa atgctatcgt cggtggtgtg
    22801 gttcctcgtg aatttattcc agccgttgaa aaaggacttg tagaatctat ggcaaacggt
    22861 gttcttgctg gatatccaat ggttgacgta aaagctaaac tttacgatgg ttcataccat
    22921 gatgttgact catctgagac agccttcaag attgctgcat cacttgcact taaagaagct
    22981 gctaaatcag cgcaaccagc tatccttgag ccaatgatgc ttgttacaat tacagctcca
    23041 gaagacaatc ttggtgacgt tatgggtcac gtgactgcac gtcgcggtcg tgttgatggt
    23101 atggaagcac gtggtaacac acaagttgtt cgtgcctttg ttccacttgc tgaaatgttt
    23161 ggttatgcaa cagttcttcg ttctgcaaca caaggacgtg gtacattcat gatggtattt
    23221 gaccactacg aagatgttcc taagtcagtt caagaagaaa tcatcaagaa aaactctggt
    23281 gaataatttc tgaaaactta tgaaaaggta ctcgaaagag tatctttttt tctttttgaa
    23341 agtggtaaat tcattgcaat ttttctcgaa aaaagatata ataattgtgt tgaaagattt
    23401 ttgcacatat gtacaagtta atcttttcac attggttagg gggatcccta aaataaaata
    23461 tttgagattt tcataaggag gaaatcacta atggtagtta aagttggtat taacggtttc
    23521 ggtcgtatcg gtcgtcttgc attccgtcgc atccaaaacg tagaaggtgt tgaagttact
    23581 cgtatcaacg accttacaga tccaaacatg cttgcacact tgttgaaata tgacacaact
    23641 caaggtcgtt tcgacggtac tgttgaagtt aaagaaggtg gattcgaagt taacggtcaa
    23701 tttgttaaag tttctgctga acgcgaacca gcaaacattg actgggctac tgatggcgta
    23761 gaaatcgttc ttgaagcaac tggtttcttt gcatcaaaag aaaaagctga acaacacatc
    23821 catgaaaatg gtgctaaaaa agttgttatc acagctcctg gtggaaacga cgttaaaaca
    23881 gttgttttca acactaacca cgatatcctt gatggaactg aaacagttat ctcaggtgct
    23941 tcatgtacta caaactgtct tgctccaatg gctaaagctt tacaagacaa ctttggtgtt
    24001 aaacaaggtt tgatgactac tatccacgca tacactggtg accaaatgat ccttgacgga
    24061 ccacaccgtg gtggtgacct tcgtcgtgct cgtgcaggtg ctgcaaacat cgttcctaac
    24121 tcaactggtg ctgcaaaagc tatcggtctt gttatcccag aattgaacgg taaacttgat
    24181 ggtgctgcac aacgtgttcc tgttccaact ggatcagtaa ctgaattggt tgcaactctt
    24241 gaaaaagacg taactgttga agaagtaaat gcagctatga aagcagcagc taacgattca
    24301 tacggttata ctgaagatcc aatcgtatca tctgatatcg ttggtatttc atacggttca
    24361 ttgtttgatg ctactcaaac taaagttcaa actgttgacg gtaaccaatt ggttaaagtt
    24421 gtttcatggt acgataacga aatgtcatac acttcacaac ttgttcgtac acttgaatac
    24481 tttgcaaaaa ttgcaaaata atttttaatt aatatgatgt aaaagaggga tttatccctc
    24541 ttttattttt ataaataaaa aataatacga atgttttttc attttgttgt agaaaattat
    24601 cagaaaagaa atgaaaactg aattatattg gctataagtt aaaataaggt ttgattttga
    24661 aagtaagcag tggtatgatt taattgaaaa aatatagagg agaagtttta tgaagactac
    24721 aaaagttttt tcagttttat cgttatctgt tgctgtattt atgatgacag catgtcagtc
    24781 aaaccacagc actaaaaaag ctgatatgtc tccagataag gtggagcaaa agacgaaatt
    24841 taaaaatatt tctctaagtt ctaataaact gttggctaag gaaaacacta tgtctgtcct
    24901 ttggtatcaa aattctgcag aagccaaggc tttatattta caaggttata atgttgctaa
    24961 aatgaagtta gatgattggt tacaaaagcc cagtgaaaaa ccatattcaa ttatcttaga
    25021 tttagatgaa acagttttag ataatagccc atatcaagca aagaatatta aagatggctc
    25081 tagtttcacg ccagagagtt gggataaatg ggtgcaaaag aaatcagcta aggctgttgc
    25141 gggtgccaaa gaatttttga agtatgctaa tgaaaaggga ataaaaattt attatgtctc
    25201 agatcgtaca gatgcccaag ttgatgcgac taaagaaaat ttagagaaag aaggtatacc
    25261 tgttcaaggg aaagaccact tgcttttcct taaaaaagga atgaaatcta aagagagtcg
    25321 ccgtcaggca gttcaaaaag ataccaattt aattatgctt tttggagata atttagttga
    25381 ttttgctgat ttttctaaat catctagtac agatagagaa caactactaa ctaaacttca
    25441 aagtgagttt ggtagtaaat ttattgtttt cccaaatcct atgtacggtt cttgggaaag
    25501 tgctatttat caaggaaaac atctggatgt tcaaaaacaa ttgaaagaac gacaaaaaat
    25561 gttgcattcg tatgattaat aagtatacga attctcttga aaacaactag gaaaatctat
    25621 gaaatctatt tgtgaaaaag taatcaataa ttacaactgt tcacaaaaat atgttataat
    25681 aaataatatc aaattatata aggagtttta ctaatggcta aattgactgt taaagacgtt
    25741 gatttgaaag gtaaaaaagt cctcgttcgt gttgacttta atgtgccttt gaaagacggc
    25801 gttatcacta acgacaaccg tatcactgcg gctcttccaa caatcaagta tatcatcgaa
    25861 caaggtggtc gtgctatcct cttctctcac cttggacgtg ttaaagaaga agctgacaaa
    25921 gaaggaaaat cacttgcacc ggtagctgct gatttagctg ctaaacttgg tcaagatgtt
    25981 gtattcccag gtgttactcg tggtgcaaaa ttagaagaag caatcaatgc tttggaagat
    26041 ggacaagttc ttttggttga aaacactcgt tttgaagatg ttgacggtaa gaaagaatct
    26101 aagaatgacg aagaacttgg taaatactgg gcttcacttg gagatggaat cttcgttaac
    26161 gatgcatttg gtacagcaca ccgtgctcac gcatcaaacg taggtatctc agcaaacgtt
    26221 gaaaaagctg tagctggttt ccttcttgaa aacgaaattg cttacatcca agaagcagtt
    26281 gaaactccag aacgtccatt cgtagctatc cttggtggtt caaaagtttc tgataagatt
    26341 ggtgttatcg aaaaccttct tgaaaaagct gataaagttc ttatcggtgg tggtatgact
    26401 tacacattct acaaagctca aggtatcgaa atcggtaact cacttgtaga agaagacaaa
    26461 ttggatgttg ctaaagacct ccttgaaaaa tcaaacggta aattgatctt gccagttgac
    26521 tcaaaagaag caaacgcatt tgctggttac actgaagttc gcgacactga aggtgaagca
    26581 gtttccgaag ggttccttgg tcttgacatc ggtcctaaat caatcgctaa atttgatgaa
    26641 gcacttactg gtgctaaaac agttgtatgg aatggaccta tgggtgtctt tgaaaaccct
    26701 gacttccaag ctggtacaat cggtgtaatg gacgctatcg ttaaacaacc aggcgttaaa
    26761 tcaatcatcg gtggtggtga ttcagcagca gctgctatca accttggccg tgctgacaaa
    26821 ttctcatgga tctctactgg tggtggagca agcatggaat tgctcgaagg taaagtatta
    26881 ccaggtttgg cagcattgac tgaaaaataa tagtcattat aatttatatt aaaggacatg
    26941 ctgatgcatg tcttttttta gttgataatg tagagttatt gaattttcct tgacttcact
    27001 tatcatttac tagtttttag atatagtggt atttggttga cactgcaatt ttcaaaagtt
    27061 tatgacgatt tcatataaat tggtttataa aagatatact agtaataaat aaaacgttta
    27121 caaaaagggt tttatggatt gatatgttag aatatatagt gaagggatag gtatgaaaac
    27181 gttaaaaaaa ttattatcta attacaaatt tgatattaaa aagtttaaat tgggaatgag
    27241 aacatttaaa acaggcttat cggtcttttt agttttattg gtttttcatt tatttggttg
    27301 gaaagggctt caaattggtg ctctaactgc tgtatttagt ttaagagaag actttgataa
    27361 aagcgtgcac tttggttttt ctagaattat tggtaatagt ataggtggct tactctcctt
    27421 agtatttttt gcattcaatg agatatttca tcaagctttt tgggttactt tattaattgt
    27481 tcctatttgt accatgttat gtatcatgat taatgttgct tgtaataata aatcaggtat
    27541 tattgggggg acagccgcat tacttattat aactttatca atcccaagcg gagagacaat
    27601 tctatacgtt tttgcacgta tttttgaaac tttttgtggt gtttttattg caatgatggt
    27661 taatacagat attgaaatac ttcggaaaaa actaaaaaat aacaaattat agtttgatgt
    27721 cataaaatct cacatgagag ttgacatcaa actttttaaa gtttataatg gtttaatgaa
    27781 aggaggccat catgaaagaa agagaactta ggcgtacaat ggctgtattc ccaattggag
    27841 ctgttatgaa gttgacagat ttgactgcca gacaaattcg ctattatgag gatcaagggt
    27901 taatcacacc tgaacgtact gaaggtaacc gccgcatgtt ttcactgaat gatatggacc
    27961 gcttactgga aataaaagac ttcattagcg atggtttgca tatttctgat attaaaaatg
    28021 aatacatgca aagacaacat aagtccaaag agaaacaaaa gtcattatca gatgccgagg
    28081 ttcgtcgact tttacaagat gagctccgta atcaagggcg tttttcaagt ccttcacagc
    28141 acattggaaa tatgcatttg taaacatgca gtcaagctag atattaagga gactatatga
    28201 ctatcacagc agaagatatt cgtcgcgaag ttaaggaaaa aaatgttacc ttcctccgtt
    28261 tgatgttcac agatatttta ggagtaatga aaaatgtcga aattccggct acagatgaac
    28321 aattagacaa agttttatca aataaagcaa tgtttgatgg atcttctatt gagggctttg
    28381 ttcgtatcaa tgaatctgat atgtatttat atcctgattt agatacttgg attgtctttc
    28441 cttggggaga tgaaaatgga gctgttgcag gtctaatctg tgatatctat acagcagaag
    28501 gagaaccttt tgccggagat ccacgtggaa atcttaagcg taatatgaaa cgtatgcaag
    28561 agatgggcta taagtcattt aacttaggac cagaacctga atttttccta tttaaaatgg
    28621 atgaaaatgg taacccaaca cttgatgtta atgacaaagg aggctacttt gacttagctc
    28681 caacagactt agcagataat acacgtcgtg aaattgttaa tgtcttaaca caaatgggct
    28741 ttgaagttga agctagtcat catgaagtag cagttggaca gcatgaaatt gactttaaat
    28801 atgatgatgt tttaaaagcc tgtgacaata ttcaattatt taaattggtt gttaaaacaa
    28861 ttgctcgtaa gcatggttta tatgctactt ttatggcaaa acctaagttt ggtatcaatg
    28921 gttcaggtat gcattgtaat atgtctcttt ttgataatga agggaacaat gctttctttg
    28981 atccagaaga tccacgtgga atgcaattat cagaggatgc ctactacttt ttaggtgggt
    29041 taatgaaaca cgcttacaat tacacagcta tcattaatcc aactgttaat tcttataaac
    29101 gtttggtacc aggatacgaa gcaccagttt acattgcttg ggcaggtcgc aatcgttcgc
    29161 cacttattcg tgtacctgca tctcgaggta tgggaacacg tctagaattg cgctcggttg
    29221 acccaacagc aaatccttat cttgctttat cagtactttt gggatcagga cttgagggaa
    29281 tcgagaataa aattgaagca ccagaaccaa ttgagacaaa tatctatgct atgactgtag
    29341 aagaacgtcg tcaagcaggt attgttgatt taccatctac tcttcataat gctcttaaag
    29401 cacttgaaga agatgaagtg gtaaaagcgg ccctaggaac tcatatctat actaatttct
    29461 tagatgctaa gcgtattgaa tgggcaagtt atgcgactta tgtttcacaa tgggaaattg
    29521 ataattacct agatttatat taatcataag aatgtttata actagtttta ggctttattt
    29581 tgtaaagtct aaaactagtt tttttgtaaa ttattgtaaa aaattgccta aaacttgtta
    29641 caggttttca tttgtctgtt atatggattt gataaactag gagtcatcag atggaggaaa
    29701 acatgaacat aaaacaatta aagtctaaaa caatgcttgg aacagtggct ttggtgtccg
    29761 ctttttcatt tgcttcaaca aatgcagatg cgaatacata taactatgca gtagatgtag
    29821 actacctagc aagtgcggag gaaattgctc aagcacatcc agctagtaat acgtttccac
    29881 taggtcaatg cacatggggt gtaaaggaga tggcaacatg ggcaggtaac tggtggggaa
    29941 acggtggtga ttgggctgcg agcgccacat cagctggtta tacagtagga acacaacctc
    30001 gcgtgggttc tatcgtatgt tggactgatg gtagttatgg acatgtggct tatgttacag
    30061 ctgtagatcc tgtcactaat aaaattcagg ttttagaatc taattatgca ggtcatcaat
    30121 ggattgataa ttatcgtggt tggtttgatc cacaaaatac agtaacacca ggggtagtta
    30181 gctacattta tccgaattaa tctataaaga actcctcaca tagacggtat aaaagtacga
    30241 tactgtttgg atggggttct tttatattct atagttatcc cagaagtaat ttgatatact
    30301 aacttatttt gctaatatag gcaattaaaa agaacggaaa aggtattttc cgttcttttg
    30361 tttatttatc aggggtaagg accataggaa taatgatagg ctcacgctca gttttttcat
    30421 ataagaatgg tcgtaaggca ttaacaatag ctccatttac tgattggata cttgcatctt
    30481 tatttttcag tgcaatacga atagcattga ataagatatg ttggctttcg cgaataaggt
    30541 ccccagattc tctcatatag ataaagcctc ggctaaggat gtctggtcca gctaaaatca
    30601 tttttgaatc gaaatcaacg gtagcaacgg caaggacaac accatcttca gataagtcgt
    30661 ggcggtcacg tagaactgcc gcaccaatat cgccaatacc attcccatca acgtagatat
    30721 cttgagcatt gaaatgtcca gcaatgcgag cagagtcgct cgttaaagct aaaacatcac
    30781 cgttttccat aatgaagata ttctctttag gaatacctgt gtcaactgct aaaccagcat
    30841 ggactttttg catgcgatac tcaccgtgta cgggcatgaa atactctggt ttaatcaagc
    30901 gtaacattaa tttttgttct tgttgaccac catggccgga ggtatggata ttgttaattt
    30961 taccatgaat aacatcgaca ccagcttctt gaatggtatt gattaattta ttgacacttg
    31021 ttgtattacc agggattggg cttgatgaga aaatcacagt atcaccaggt tgcaaagtaa
    31081 cttgacgatg agtaccgtta gcgatacgag caagtgctgc catagactca ccttgactac
    31141 ctgtacacat aattaaaact tcactagcat gaagatgttt aagttcgctt ggctcaatga
    31201 aggtaccttt tggaacttta atatagccaa gttcaatacc gttaacaatt gccttttcca
    31261 ttgaccgtcc aaaaacagca attttacgac cagttttaac agcagcttca gcagcctgtt
    31321 ggagtcggaa gatgtttgaa gcaaaagagg cgaaaataat acgtccctcg ataccttcga
    31381 taatcttcat aatagattgc ccaacaattt tttcggaatt agtgaaggtt ggaacttcag
    31441 catttgttga gtctgataaa aggcagagga caccgtcttc acctagagca gccatacgat
    31501 gtaagtcagc gggctcccca acaggtgtaa agtcaaattt gaagtcacct gtacaaataa
    31561 ccttaccttg aggggtatga ataacaatac ctaatggttc aggaatggag tgagtagtgc
    31621 ggaaaaatgt aacgcttaag tttttaaaag tcagctctgt attagcgtgg atttcgtata
    31681 atgtagcgtc acgaagtaaa ccatgttctt ctaacttacc tttgattaga gcaagagcga
    31741 gagggcctgc ataaatcggt aaattagctt gttttaatag aaaaggaata ccaccgatgt
    31801 ggtcctcgtg cccatgagtt ataacaaggg ccttgatacg gtcaatgttt tcaacgatat
    31861 atgaatagtc tgggataaca taatctattc ccaaaagatc atcttcaggg aatttaatac
    31921 cagcgtctac aataataatt tcgtcttgat attcgatacc gtaagtattt tttccgattt
    31981 caccgagacc accaatcgca taaaccccaa cttcttcggg ttttaagttg atatttgaca
    32041 taataagttt atcataatgg gtagattact ctagctatta tgtttaactc ctttctagaa
    32101 ctcagtgagc tcgaaaacac cagtttcttt ttcatattca aggtgtttgt ctgaaagtaa
    32161 ctcgatgaat tcgatattgt aagctgtatt ttcttcaact agtttacgtg cagcaatgcg
    32221 accttctaat tcattagctg catcaatagt aacgtaaagt gttttggttt gctcacgacg
    32281 tgggttgcgt tcttttgttt cttgatagaa aactttataa atcataataa tcctttctta
    32341 tctctcggtt tttctaacta taaaaaaatg ggtcaatgcc gttaagtgag acaatgacaa
    32401 accattatta agtttttata gagtaataga attaaccttg attcgttgca attatcatga
    32461 ttatatcata aaataagagg tagaatcaag taagggaagt taaatgatga aagttttagc
    32521 ctttgatact tcaagcaaag cactatcagt ggctgtacta aacaatatgg aatgtttagc
    32581 gactgtcact atcaatatca aaaagaatca tagcattaat ttgatgccag ccattgattt
    32641 tttaatgcaa tcaattgatt tagaacctca agatttggac cgtatcgtag tagcagaggg
    32701 tccaggatct tatacgggct tacgtgtagc tgttgctaca gcaaaaatgc tagcttatac
    32761 gcttaagatt gacttagttg gagtatctag cctgtacgct ttaacaaatg gattttcaga
    32821 aaatgattta ttggtaccac ttatagatgc acgacgtaac aatgtttatg ttggtttcta
    32881 tcaaaatgga gatactgtta aaccagactg tcacacttct cttgaagaag tcttacaaga
    32941 ggtggggaat aaagccaatg ttcattttgt cggagaggtt gcagcatttg ttgaccagat
    33001 taagaaagtt ttaccacatg ctaaaattac agaaacttta ccttgtgcag tggcaattgg
    33061 gcgcaaagga caaaaaatga aaagcgttaa tgtagatgcg tttgttccac gatacttaaa
    33121 acgtgttgaa gctgaggaaa attggttaag aaaccactgt gaaacgaata cagaagaata
    33181 tattaagaga gtttgagatg gaatcatcag aacaagcact ggctatatgg agcgtactat
    33241 ctgatgtcta tgacaaatct ccgtggagtt tatcgcagat ttcagaagat ttaaaaaaag
    33301 attcaacaga ttattttttt gtttataatg acggtgaagt cattggtttt ttggctcttc
    33361 aacaattggt aggtgaagta gaaattacta atattgctgt taaaaaaaat tatcaaggta
    33421 aaggttatgc ttaccagtta atgtcaatga tcgctgatat agaggtgcca gtttttcttg
    33481 aggttcgtta ttcaaatatc gtagcacaaa aactttatga gaggtgtggt tttgtagttt
    33541 taggaaagcg caaaaattat taccatgatc caattgaaga tgctatagtg atgcgcaagg
    33601 gataaatagt tgatgataga aaggatatga atagtcctca atactaatgg aaaagacgat
    33661 attgatggat taatatcatt atgaaagata gatatatttt agcagtggaa agttcatgtg
    33721 atgaaactag tgttgctatt ttaaaaaatg ataaagaatt actagctaat attattgcaa
    33781 gtcaagttga aagtcacaaa cgttttggtg gtgttgttcc tgaagtggca agccgtcatc
    33841 acgttgaagt agtaacaacc tgttttgagg atgctcttca agaagcaggt attgttgcta
    33901 gcgatttgga tgctgttgct gtaacatatg gtccgggatt agtaggagcc ttattggtag
    33961 gtatggctgc agcaaaagct ttcgcttggg caaataaatt acctctaatt cctgtaaacc
    34021 acatggcggg tcatttaatg gcagcacgtg acgttaagga acttcaatac ccattgttag
    34081 ctttgcttgt cagtggagga catacagaat tagtatatgt ttctgaaccg ggagattaca
    34141 aaatagtagg agaaactcgg gatgatgctg ttggagaagc ttatgataaa gtaggccgtg
    34201 ttatgggctt aacttatcca gcaggtcgcg agattgatca gttagctcat aagggtcaag
    34261 atacttacca ttttcctaga gcgatgatca aagaagatca tcttgaattt tctttttctg
    34321 gattaaaatc tgcatttatc aatttacatc ataatgcaga acaaaagggt gaagcattgg
    34381 ttcttgaaga tttatgtgct tcctttcagg cggctgtttt ggatatttta ttggctaaaa
    34441 ctcaaaaagc tttgttaaag catccagtga aaactttagt cgttgctggt ggagttgcag
    34501 ctaatcaagg acttcgggaa cgcttggcta ctgatatttc tcctgatatt gatgtggtta
    34561 ttcctcctct tagattatgt ggggataatg caggaatgat tgcattagca gcagcgatag
    34621 agtttgaaaa agagaatttt gcttctttaa aattgaatgc caaacctagc ttagcttttg
    34681 agagtttata gtgttcataa tatcaaaacg cttgttttga tattttttat ttgttataat
    34741 gattgaatat tcaaaaatat tagcaaatga tattgtcgct atttttttaa ctaataaagg
    34801 agttaaggaa tgttacattt tgaaaatgat tataataaag gagctcatac agagcttttg
    34861 aatgccctta ttgaaactaa tgatcaaggt ctatcgggat ataggacaga tagttactgt
    34921 caacaagcgg cagataaaat tcgagaagtc tgttcctgtc cacaagctga agttgagttt
    34981 ttagtgggtg gtactcaaac aaatcaagta gttatcagta gtatgcttgc ttcttatgaa
    35041 ggagttattg ctgcagaaac aggtcatgtg agtagtcatg aagcaggagc catagaattt
    35101 tcaggtcata aggtattgac tttaccttct cataatggga aattactcgc aagcgaagtt
    35161 gcaacttata ttgagacttt ttatgctgat ggtaattacc aacacatggt ttttccagga
    35221 atggtttata tatcacatcc aacagaatat ggaacacttt atagtaaagc agaactagaa
    35281 gagttgtcga agatttgtaa acattatcaa atccccttat ttattgacgg tgctcgtctg
    35341 gggtatggtt tggctgcgaa agatacagat gttgatttcc caacgattgc agccttatca
    35401 gatgtttttt atattggcgg aactaaaatg ggggctcttg ctggggaagc agttgttttc
    35461 acaaagaaaa atcgtccaaa acagtttact acaattgtaa agcagcatgg tgcgttatta
    35521 gccaagggaa gattgctggg acttgctttt gatcgtttct ttacggatga cctttactta
    35581 aaaattggaa agcatgcgat cgacttagca gaggaattaa aaatcatttt agaagaaaaa
    35641 ggttactcat tctatttaaa atcaccaact aatcaacaat ttattattgt agaaaataca
    35701 aaactagcag atttagcaaa gaatgtagct tatagttttt gggaaaaata tgatgaccat
    35761 catactgtaa tacgtctagc aactagttgg tcaactagtc gcgaagatgt aactgcttta
    35821 agaaatgtct tgtgagtttt taataaaaga aatattattc agtagtgact attacaaata
    35881 atagtcttgt ttttgggaat taactaaaag aattttatca gtatttataa taataaaaag
    35941 ctcggtatct tggcataccg ggctttttga aaataaataa attggcatct atgatttgag
    36001 ataagaaaaa ttttttcatc ttttattatt agtcatttaa ggattgatga gatcctacat
    36061 tcctatgata ataaaatttt gaagaagttt gtaatagctg agtaaatact caacaggtta
    36121 aataattaca atgtattaac tactcgtcca cactcaatag aatagtcata aaaagtaact
    36181 atttgtcttc acgaattacc agcttgcttt tttaatgcca ggaatttggc ctttgtaagc
    36241 taggttacgg aagttaatac gactcatacc gaatttacgc atgtaagcgt gaggacggcc
    36301 atcgattaaa tcacgatttt taagtcggtt agggttagaa tctttaggaa gtttacgaag
    36361 tgcttcgtaa tcaccctttt cttttaactc acgacgtagt tcagcatatt gttctacgag
    36421 tttttgctgt ttttgaaatt tagcaatttt tgatttctta gccatgattt ctcctttccc
    36481 aaaaagattt ttctatccca aaacagggtg cctagttaac tggtaattaa ccagtaaaca
    36541 agacatgatt ctattatact cttttcagct aagaatgcaa ggaaaatgtt aactggataa
    36601 gtattatatt gtaatggtga tgaatgtgtt acaataacag tatgaatgaa agatatatcc
    36661 gatttaaccc taatatcgcg aaacaaaaac ccagtaataa agacaaatgt ccattttgtg
    36721 ataaaagcca attaggaaaa atcctagacg tgaaagacga tatgatctgg gtagaaaata
    36781 aatacccaac tttagaagaa acctatcaaa cattggtgat tgaatcaaat gatcacaatg
    36841 gggatatttc agtttatagt gagtcaaaga tgcgccaatt gttagattat cttttgtcaa
    36901 aatggcaatt aatggaagaa agtggtcatt accgttcggt cgttctttat cgaaattttg
    36961 ggcccttatc tggcggaagt ttacgacatc ctcatagcca aattgttggc ttaaaaaacg
    37021 tagatgctta tgaaacgttg aacttagatt ggtttgatgg tatcgaggtt tgttcggaaa
    37081 cgagtcaaca agcagagatt agtattgcca atcgtcctat gcttgatttc acagagttca
    37141 caattaaagt atccaaattg gaaaacgcta ttttgattgc agatagttta ccaattttgt
    37201 gtcattatat tttaaatgaa ttcttgggag ggcgctgtaa atcttataat ctctttttct
    37261 ataagcatga tgggggtctt atttgtaaaa ttgttcctcg ttttatagta tccccttatc
    37321 agattgggta tgctattcat caggtaaatt taccagatga attggaaaaa atcaaagaag
    37381 ccttaagagc caaatattat ctttaaattc tatataaaaa gctgacggaa ttgttcagct
    37441 ttcatgtatt aatagtgtaa gctaccagct acccagcctg tacataaggc agaagtaatg
    37501 ttaaaccctc ccgtatgagc attgatgtct aggacttcac cagcaaaatg taaaccagct
    37561 acctttttac tttcaagtgt tttggggttt atttctttaa gatcaactcc accttttgtt
    37621 acaaaggatt ttgctagaga catcttgcca gttactaaaa taggaagttt tttaagtttt
    37681 tgaattaacg tttctaagtc ttttacagat acttgtttta ctttttcggg taagtcttca
    37741 gcgtaaaatt cagctaaacg ctcaggaaga agtattttaa gagcgttttt caaagatttt
    37801 tctctttcat tttggaagtg tgtttccaat tctttaacag agatattggg caaaacatct
    37861 aggtatatag tttctccgcc tttgacaaag ctggaaattc gcaaggctgc tggtcctgat
    37921 aatccaaaat gagtaaagag aagatcgtgt gtaataatat gcttttcaaa tgacaaagtg
    37981 acatcatcta aggagatacc ttgtagtttt ttatggggaa aatcagttaa aagagggctt
    38041 tcggcggctt ccatatctgt gacttctaat ttaaagtgac gagcaatgtc atgaccgaaa
    38101 ccagtagaac ctgtagacgg ataggattta ccaccagttg ttacgacaag tttatcggat
    38161 gcgaactgtg tatctttagt tttgatatag aatgaatctc ctatcttttt gacagagaca
    38221 acttctgtgt cagtcataat ttgaccacca agttctttta ttttattttc aagggcgtta
    38281 atgatagtac gagatttatc agtagtaggg aacatacgcc catgatcttc ttctttaagt
    38341 gtgacgccgt tgtcttgaaa gaagttaata atatcatggt tatcaaattg tgagaagacg
    38401 ctatatagga aacggccatt tccaggtata ccagctaaga gctcatcaag gtttccatta
    38461 ttggtaacat tacagcggcc accacctgtt ccagctagtt ttttgcctaa acgtttattt
    38521 ttctcaataa gaagtgtttt gttgccgtaa aaatttgagg aaatagctgc catcattcca
    38581 gcaggtccac cacctataat tattgtgtca taatgcttca taatcttatt ctagcatata
    38641 aatagagaaa tagacgtcaa aatgtcttgt ttgacacttt aagttgacag taaattgaca
    38701 ctagattagg attgtgatat tcttttataa gaaaatcagt acaaaagagg aagttatgac
    38761 agttgaaaag acggttagtg aattagctga aattttaggg gttagtcgtc aagcgatgaa
    38821 taacagagtt aaaacactcc ctgaagaatg tgtggaaaag aatagtaaag gcgttacagt
    38881 tgttaatcgt gatggcttga ttaaattgga agaaatctat aaaaaaacta ttttggaaga
    38941 ggaaccaatt gatgaagaag catcaagacg agaactttta gaaattctcg ttgatgagaa
    39001 gaatactgaa atcacacgtt tatatgaaca gttaaaagca aaagatattc agattgcttc
    39061 aaaagacgag cagttacatg taaaagatat tcaaatagca gagaaagata agcaattaga
    39121 tcaacaacag caattgactt taactgctat ggaagatacg caacgtctgc aacttgagtt
    39181 aaatgaagct aaagcagagg ttgaagagat tcaagaagcc aaagaagaaa aaattcaaga
    39241 attagaagct gttaaaaaaa gtttctttgg acgtttcttc aataaaaaat agaataaaag
    39301 cgtaaggagt ttccttgcgc tttttaggag aaataatgaa acaattacaa gaatatatag
    39361 cttttgattt agagtttaac acggttggtg agcatagcca tattattcaa gtgtcgtccg
    39421 ttaaatattc taatcatcaa gagattgctt tatttgatac ttatgttcac acaaaagttc
    39481 cattacaaag ctttattaat ggtttaacag gtattacagc tagagatatt ataggggctc
    39541 ctaaaattga gacagttttg acagactttc agagttttgt tggagacaca ccgctgattg
    39601 gttataatgg gcataaatcg gatttacctc ttttagttga aaatggtttg gatctaacta
    39661 gtcaatatca ggtggactta tatgatgaag cttttgtgag acgtagtaca gatttaaatg
    39721 gtattgtcaa ccttaaattg actactgttg ctgacttttt aggaataaag ggtaaggcac
    39781 ataatagctt agaggatgcc cgcatgactg cgcgtgttta tgaaaaattt ctggacttgg
    39841 atgaaaataa aatctattta aaacagcaaa aagaggtagc agttgatagc ccatttgcga
    39901 ctttaggaaa tttgtttgat taattgttgt agttttattt aaagttaaaa atacttagcg
    39961 ttattacttg cttattacct taggtcattt gatatagtaa aggtgtttct gataaagaag
    40021 tgtttactat attaaaggag gtgtgaaaca tgtcagaaaa atattccact ggtgacttag
    40081 ctaaagaagc aggagttact gttcgtactg ttcagtacta tgacaaaaga ggaattttat
    40141 ccccatcaga actatctgaa ggaggtagga gggtttatag tattgctgat ttagaaaagc
    40201 ttagacaaat tatttattta cgagatttag atttttcaat tgataatatc aaaaatctat
    40261 ttactgaaga caatgctagt cagattttag agcttttttt acaagtgcag ataagagaat
    40321 taaggctatc tattgatagt aaaaaagaca aacttgacaa agctgtcaac ctactaaaaa
    40381 ccgtagaaaa acaagacagt aaaacattag gctatctttc tgacattgtg cttatggaag
    40441 aaaacaaaag aaaatggggt aaactacaaa aaagcatagt gctacaagtc attatgatta
    40501 ttatggtcta tattacgtta ataatggcag cagtttctct aaatcaaaga tggcttatgt
    40561 ggatagcagt aacagtattc ttaatagcta tgaatggtat ggtttggtat tttaaacaac
    40621 aggttgttta tctttgtcca aattgtcatc aaacctttac accgacatat caagcattta
    40681 atctagcgaa acatacccct aagacgcgaa aagtattctg tccacattgt caccaaaagt
    40741 cacattgtat agagttagcc agagaagaac gttaaagaac tcccttgaat tcaagagagt
    40801 tctttttatt tagaataagt tggaatacta taatattaac tatatcaatt agcagaatac
    40861 atataatcgc gtgtcatagg catagtgtct tttgaaacac cttttgaaag caagtattga
    40921 aaaatatcaa tattgccaga ttcaaaagag gccgcacaag attgaagata gaggtcccac
    40981 atattgataa aacgcttatc atgagttttc tgaacttcag gaagggcatt atggaaattt
    41041 tttgtccata attcgagagt cttttgatag tgacgacgaa gcggttctaa gtcagcaatt
    41101 tgaagtccag cactagctat atgatttaaa ttttcagtta aacgagggat atatcctcct
    41161 gggaagatat acttattaat ccaagaatta gttccactac catgattacc acccacttgt
    41221 ccagtaatac catgaataag agctagacca ttaatgttga gtcttttaga aatagtttgg
    41281 aagtattgag ataaattttc ttttcctaca tgttcaaaca taccaacact agtgatataa
    41341 tcgtatgttt catgaatgtc acgataatct ttaatgagga ctgttacttt attttccaat
    41401 ccttcttcct tgatacgctt agtaatgaag gaagcctgct cttcacttaa ggtgatacct
    41461 gtcgcattta agccatattc tttagcagca gtaatgataa gtgtccccca gccacaaccg
    41521 atatctagta atttccctcc cggatgagca tttaatttat taagaatatg gtgtactttg
    41581 tttagttgag cttgctctag actgtcattt tcatgtttaa aataggcgca tgaataagtc
    41641 atggtatcat caagccaaag tttatagaaa tcatttccga tgtcataatg cttttgaatg
    41701 tcggatttac tgtgtttttt atcatgaaat tgtttaggaa tcaatttgct aaatttacta
    41761 ttacgtagaa agctgtctcc acagcggtaa gctgataaaa ttaattcttg tatagaacca
    41821 tcaatttcaa tgtctccatc cataacggct tcacccaata ctatagaagg gtcttttgat
    41881 agctccgaga cggagaaatt tttgtttagt ttaagtttga cgtggggatt gttaccatta
    41941 taagtttcaa tattcccatt tggataagtg acttgtaagg gtatatcaaa tgtactctta
    42001 atcaactgtt ttatgatcag actatccata accatggtta gtcaccttct ctctaatatt
    42061 ttcaatgtta ttatacctct ctgctggttt tttgcaaata tttttttgaa aataaaactt
    42121 gttttcgtga aaaaatgaaa gaagtttgaa ataaaaatca catttacagg tgattcagta
    42181 aatgtgattt attttttaga atggcatttt ccccgcaaat gctcccataa ttttttttgt
    42241 agcatcgtca acttggctta aagcgtcatt gatagcttga gttgtcatgt cttgtagtgt
    42301 ttcaatatct tcagggtcta ccacagcttc tttgtaatca attgaaatta gctttttatc
    42361 gcctgtaaat gtcacagtaa cgagctcttg agcagatttt ccagtaaact ggctagctgc
    42421 taaatcagct tgcttttgtt ccatttgttt ttgaagcttt tgtgcttgac gcatcatatt
    42481 ttgcatattc atcataagat tttattctcc ttagtaaaag tgattactgt aacattatag
    42541 cataggaaag ggggtgtgtc atagggatta gataatattg gatttttgaa ggtctatact
    42601 agttcatttg gctaaattac caagagaaag aatgtttcca agattgtagt aagattctta
    42661 attgtttcaa aaataaatta ttctataata agataattaa cgataaaaaa atatttacaa
    42721 gattttttat atagaaggag atttgaatag tttaactgtt caaatgatgg aaagatgaag
    42781 gttcagtaat tgtttagtca gatatgactc ttaactagat attttaaaaa agtgtaaata
    42841 tatgtttaaa cactcctaaa aagtaacatc gctcatctag gagcaagtga attatttagt
    42901 ttttgaggat tacttctttt ggcttttatt ttaccattaa tgggtaatgt tattgatgca
    42961 ttaagttttt caaccgcatt taagtgtcta ggtatttttc attccttttt tagttagtat
    43021 tgggatatta aaatgaccag aaatatcatg agataataac taatcagaca gcttttattt
    43081 gctagttatt ttttagattt attgattttt ctgtttagaa aagatatttt gcactttgtg
    43141 ctgttataat ttatattaat tccgacttaa aagtctcaca atgacgcttt tttagtagtt
    43201 atttgagata aaaattatat aatataatta aaatattttt aaacagctag tttacggctg
    43261 tagttagttt ggataataat tgagaatgta aagaaatttg taatatttaa tctataagga
    43321 tgaattatga agaatataat tttagaaact agtcgattta ttttattaga atgggatagc
    43381 ggagatttat ctgagttaaa gtcattttgc aagatgaaga agtgatgtgg gcttatgata
    43441 agagttttac tgatcaagat tgtcagtact ggttagattg gaatatgaat tcttacaggt
    43501 ctgatggatt tggtctgtgg aaaatcattc ataaagaaac ttcagaggtc gtaggtgaat
    43561 gtggcattac aatgcaaact gttgaaggag aaaattaccc tgaaataggt taccatctta
    43621 aaaaagagca ttggaataaa ggaattgcga cagaagttgc tagggcagta cgagattatg
    43681 ctttcgaaga gttaaacctt gaggaactca ttatcataag tcgagatatt aatttacagt
    43741 caatgaatat tgccataaaa tcaggattta ctattaaaaa aagatttttg aagaatgata
    43801 ggtttcctca ttttatgttt tacttgaaaa gataggaatg tgatatgtta ccttattatg
    43861 ttgtgaatgc ttttaccaat aaggcgttta gtggcaaccc tgcgggggtt gtggttttaa
    43921 atgaaccgat taaagaggat ttaatgcaga aaatagctaa agaaaataaa ctatctgaga
    43981 ctgcatttat tttaaaagaa aaagatctat ataagattag atggttcaca ccagaaaaag
    44041 aagtggattt atgtgggcat gcgactctag caagtaccta tgttatttca cgttatcttg
    44101 aagatgattt aagtcatatc actttctctt caaagtcagg acttctagaa gtaactatta
    44161 gtggtgaaga tataacctta ttttttacaa tggttgacgt taagcaagtt ccaataacag
    44221 cgatgatgag agaatcgatt gaacctacta tcctggaagc ttatcgaaca gcagatgaag
    44281 agcatcttgt tttatatctg gaaaatcaag aatctataga gaatttcaaa attgatttag
    44341 agaaaattag tcaattagca ccacatggag tgacacttac agcccaaagt tcagaaaaaa
    44401 atattgatta tgttcttcgt tactttgcac caaattatgg tatcaatgaa gatcctgtca
    44461 ctggctcagc acagacacgt ttagcaccaa tttggacgaa aaagcttgga aaggaacaat
    44521 tagtatccaa gcaattatct gaaagacaag ggttgatgat gattgagttg gaagagaatc
    44581 ttgttaaaat atcaggacaa gctaatttat atcttaaagg caatattgat atttagtact
    44641 tgaaagttaa caattttagt gaagtaggag tatattaatg agaaaacgag aaatgaaact
    44701 cgaactatat aataataaat ggccagagaa gtatcaagaa attaaagaag aacttactaa
    44761 actattaagt ggactagaaa ttgaatgcca tcattttggg tcaacttcaa tcaaaggtat
    44821 ggcctctaaa ccgataattg atgtactcat attcgttaac aatattaata tggtagatac
    44881 gtataataat tccatgttga attctggata tgttgctaaa ggtgaaaatg gaattctagg
    44941 aagaagatat tttgttaaat atgcaaaaga caaaataaat cacttagtac acctacattt
    45001 ttatgaaaag gaagatccga aaggtttaca agagttacaa tttagagatt acttaataga
    45061 aaataagact gcttttgatc gttatttggc tgtaaaagtc gaagcgagta agaaatataa
    45121 agatatgcca gaagcctatc aaaatcataa gtctacggtt attgatgcca ttaataaaga
    45181 gatagtaaag acaaaagtct ctaaataaat tttgatagta aattaattaa ctgttcgact
    45241 taagagaaaa tttataggta ataaaaaatc gttttacatt tttctgtaaa ccgattttta
    45301 ttgtgtagaa gatactgtca ttgactaatt acatcatatt gatgtaggtt taaatgattt
    45361 tacattaatt tataaaatta tgtaaggcct taaataaata tctctgacta ttacatagca
    45421 taaagaaacg tagaacgagc cttgcatagt catcataaga tctcattctc cttattaaaa
    45481 gcaattaaaa taatttgctc taatagatta gagcaaggag ggggcaaggc taaaaagaga
    45541 ttcagatagt gttattcaat gtttatattc tacaaatgta caagataatt cttattttag
    45601 cgatgaagaa gaatttctat atatcaaaaa cattatttga taagtattca attagctcca
    45661 gtgattctac atcaccaaat agatattctg atttttcaag gcttgctatt ttagtcatat
    45721 catcatcggt taataatatg tcaaaaaagt ccatatttcc cataatacga gctttgtgca
    45781 cagtttttga aatgactgtt actttgtttt tcacttccca agctaggatt atttgagaca
    45841 cagtttttat tatagcgttc cgcaatctct accaaagtag gatgctgaaa gattccgtat
    45901 ttcgcttctg caaatggtcc ccaagccata gtatggatgc cctttttgct taaccactct
    45961 acttgatctt ttgcaggatg aaaagggttc atttgtatct gattgaccgc agggataatc
    46021 ttgttatgta agattaaatc ttgaactcgt ccatttgaaa aattagaaac accaattgct
    46081 agagcttttc tctcctcgta ggctttttct aatgcacgac atgccccata ataatccccc
    46141 attggttgat ggattaaata aaggttaata tagtctagtt gtaacctttt taatgctcta
    46201 tcaatagcag gtccagcctt ttcctcagat gcatcttgga tccaaagttt agtcacgaca
    46261 aaaatttctt ttctggggat tcctgatttc ttaatagcac gaccaactgc ctcctcatta
    46321 aggtatccag aagctgtgtc aatgaggcga taaccaactt gaagtgcatc taagacggat
    46381 tgttcgcact ctagagggtc atctatttgg aaaacgccaa agccaagact tggtattttt
    46441 actccattat ttaatataaa aaattgattt aatggcatta tggttaatcc tttcctaaat
    46501 aatttttcat cattgatgct atccattctt gatcagtcat agacttacgg tagttgataa
    46561 attcttctga cgcaacaaga taacgccatt gtttaatgtt atcagtagca gcgatgtaaa
    46621 tagtttctgc taccaaatca ggacttgaac gattagaaga gactggttca gtcatatcct
    46681 ccaggtattt ttctgcatat gttgaatatg ggctttcttt tacaccatct gttatttcta
    46741 aagaacggtt ccaaaaatct gtttgtatcc cgccaggttc aatcaactta caataaatat
    46801 taaagggata aagttcatag ctgaggccct ccattaatcc ttccactgcc tattttgctg
    46861 cgtggtaggt tgctgttaat ggtagtgtga ggacacctcc taccgaagaa attgtaataa
    46921 tactaccgcc tccattttcg cgtaaaagtg gaataaattg ttttgtaaca aaagttacac
    46981 ctaacgataa agtatcgtat gcccgacgta tttgttcttc agatgataat tctaaagggc
    47041 caaagagtgc ataaccagcg ttatttagta aacaatcaat agttttgtat tcttctttga
    47101 tttctaaaaa tgctttttga attgaaaaag agtcagttac atctaactta aaaactttga
    47161 tatttgctaa ttgagataga tctgtttcct tttcgggtct tcacattgtt gcggctactt
    47221 gccatccttt ttttgcaaaa aatttggctg ttgcttctcc aatgccagaa gaagcttctg
    47281 taattacaat tgtttttgtc ataatatttc tccttatctt attgtcattt taacaagtgc
    47341 tctcttgctt gggaatttgt aaaatgttat gatagcgtta agtcaaaact ttatatttga
    47401 tgatatctag gagactatga catgtatatt caacaattag aaacgtttgt aaaagttgca
    47461 gaagaaggtt catttagcaa ggctgcaaaa aagctttata tttcaacttc tgctgttgca
    47521 cagcaaatca atttattgga aaaccggtat cagtttaaca cctgcaggtc agtctttata
    47581 cacggatggt cttaatatta tcaagatgtc taaactagcg attgagaaag gtcgatctat
    47641 tgctagtcaa aaagaaatta ttaaaattgg aacagcagat atttttaaac ctcgtttgtt
    47701 aatgaaattt gtcaattatt ttaaagagca catttttccc tatgactttg cctttccgca
    47761 cacttcggaa gctgagtgtg atgtagttga agcgatatat tgtgatattg cttggaaggg
    47821 acattgccaa ttctacgaac tctttcaaac tcctttagca gttgcggtta atagtaatca
    47881 tccattagct ctcaaaaaaa ggtaacactt tctgatattg tagaatttag tatattaatg
    47941 cctcaaaaaa atgccttaac gggaatggat cagtttagaa atgcattgtt aagtgaattt
    48001 tcccaggcaa aaattattga tgtaccagta ataggtcagg aaacatttat gatgtgtgaa
    48061 ttggaacccc atgtctttat aactgagaat gtatttgctg atgtacaccc aaacttgata
    48121 actattccct tggagagtga attctcctta ccttatgatt tgatttatag taataatcca
    48181 agttcatcaa cattaggatt tattaaaaca attgcagatt ctaagttgac attttcgatc
    48241 gattaattgt aactttatga ccaatttttc aaaatatttt ctgtgctata atgaaatgaa
    48301 agtgtcatac actttcattt ttttgtattt taatttaaac aacataaagt tgcaaactta
    48361 ttt
//
LOCUS       DATRSD010000004        47596 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRSD010000004 DATRSD010000000
VERSION     DATRSD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47596
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.004"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..86)
                     /locus_tag="VBD85_000173"
     CDS             complement(<1..86)
                     /locus_tag="VBD85_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7132752.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISV"
     gene            260..1249
                     /gene="dhaK"
                     /locus_tag="VBD85_000174"
     CDS             260..1249
                     /gene="dhaK"
                     /locus_tag="VBD85_000174"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO7132753.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1287..1865
                     /gene="dhaL"
                     /locus_tag="VBD85_000175"
     CDS             1287..1865
                     /gene="dhaL"
                     /locus_tag="VBD85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO7132754.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSKSTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            1865..2239
                     /locus_tag="VBD85_000176"
     CDS             1865..2239
                     /locus_tag="VBD85_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO7132755.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2249..2962
                     /locus_tag="VBD85_000177"
     CDS             2249..2962
                     /locus_tag="VBD85_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7132756.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     GPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3227..3631)
                     /locus_tag="VBD85_000178"
     CDS             complement(3227..3631)
                     /locus_tag="VBD85_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7132757.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(3770..4483)
                     /locus_tag="VBD85_000179"
     CDS             complement(3770..4483)
                     /locus_tag="VBD85_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7132758.1"
                     /translation="MKTVKEISHISGISVRTLHYYDEIDLLSPSFVGENGYRYYDDES
                     LIKLQEILLFKELEFPLKKIKEIMDSPNYDRNQALLDQIRWLELKKQRLEEVIEHAKS
                     IQRGKNMSDFTAYNQEELEAFQEEARTRWGDTDSYKEFENSHSKNDFSMISQAMSQIF
                     KDFGQLKELSPTDEKVQKQVQILQDYITAQFYNCTNDLLASLGIMYIQDERFQKSIDN
                     WGGQGTALFVSKAIDSYCH"
     gene            complement(4576..5685)
                     /locus_tag="VBD85_000180"
     CDS             complement(4576..5685)
                     /locus_tag="VBD85_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO7132759.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRSQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5682..5933)
                     /locus_tag="VBD85_000181"
     CDS             complement(5682..5933)
                     /locus_tag="VBD85_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132760.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(5956..6690)
                     /locus_tag="VBD85_000182"
     CDS             complement(5956..6690)
                     /locus_tag="VBD85_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7132761.1"
                     /translation="MATYETFAAVYDAVMDDTLYAKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(6756..7112)
                     /gene="rsfS"
                     /locus_tag="VBD85_000183"
     CDS             complement(6756..7112)
                     /gene="rsfS"
                     /locus_tag="VBD85_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO7132762.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7114..7635)
                     /locus_tag="VBD85_000184"
     CDS             complement(7114..7635)
                     /locus_tag="VBD85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7132763.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAVLLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(7698..8285)
                     /gene="yqeK"
                     /locus_tag="VBD85_000185"
     CDS             complement(7698..8285)
                     /gene="yqeK"
                     /locus_tag="VBD85_000185"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO7132764.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQPDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8282..8914)
                     /locus_tag="VBD85_000186"
     CDS             complement(8282..8914)
                     /locus_tag="VBD85_000186"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO7132765.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     GISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9044..9361)
                     /gene="yhbY"
                     /locus_tag="VBD85_000187"
     CDS             complement(9044..9361)
                     /gene="yhbY"
                     /locus_tag="VBD85_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO7132766.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9454..10572)
                     /gene="yqeH"
                     /locus_tag="VBD85_000188"
     CDS             complement(9454..10572)
                     /gene="yqeH"
                     /locus_tag="VBD85_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO7132767.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGQDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10572..11132)
                     /locus_tag="VBD85_000189"
     CDS             complement(10572..11132)
                     /locus_tag="VBD85_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO7132768.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASHRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11216..12130)
                     /locus_tag="VBD85_000190"
     CDS             complement(11216..12130)
                     /locus_tag="VBD85_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO7132769.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12260..13702)
                     /gene="gatB"
                     /locus_tag="VBD85_000191"
     CDS             complement(12260..13702)
                     /gene="gatB"
                     /locus_tag="VBD85_000191"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO7132770.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(13702..15168)
                     /gene="gatA"
                     /locus_tag="VBD85_000192"
     CDS             complement(13702..15168)
                     /gene="gatA"
                     /locus_tag="VBD85_000192"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO7132771.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15168..15470)
                     /gene="gatC"
                     /locus_tag="VBD85_000193"
     CDS             complement(15168..15470)
                     /gene="gatC"
                     /locus_tag="VBD85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO7132772.1"
                     /translation="MKISEEEVRHVANLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15608..18253)
                     /locus_tag="VBD85_000194"
     CDS             complement(15608..18253)
                     /locus_tag="VBD85_000194"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO7132773.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQLGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18266..19096)
                     /locus_tag="VBD85_000195"
     CDS             complement(18266..19096)
                     /locus_tag="VBD85_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO7132774.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19107..19757)
                     /locus_tag="VBD85_000196"
     CDS             complement(19107..19757)
                     /locus_tag="VBD85_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7132775.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            19925..20890
                     /locus_tag="VBD85_000197"
     CDS             19925..20890
                     /locus_tag="VBD85_000197"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO7132776.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVIKEALTFSKDIGMVPLHIHKEQ
                     PGYILNSILVPFLESALALYYDKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTNSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(20937..21485)
                     /locus_tag="VBD85_000198"
     CDS             complement(20937..21485)
                     /locus_tag="VBD85_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7132777.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPESNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21552..22337)
                     /gene="codY"
                     /locus_tag="VBD85_000199"
     CDS             complement(21552..22337)
                     /gene="codY"
                     /locus_tag="VBD85_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO7132778.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDDYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22463..23674)
                     /locus_tag="VBD85_000200"
     CDS             complement(22463..23674)
                     /locus_tag="VBD85_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7132779.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            23941..24393
                     /locus_tag="VBD85_000201"
     CDS             23941..24393
                     /locus_tag="VBD85_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7132780.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24449..25831)
                     /locus_tag="VBD85_000202"
     CDS             complement(24449..25831)
                     /locus_tag="VBD85_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7132781.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQRYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVRENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQKVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            25900..26862
                     /locus_tag="VBD85_000203"
     CDS             25900..26862
                     /locus_tag="VBD85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO7132782.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(26897..27775)
                     /gene="aroE"
                     /locus_tag="VBD85_000204"
     CDS             complement(26897..27775)
                     /gene="aroE"
                     /locus_tag="VBD85_000204"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO7132783.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALGIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(27873..28787)
                     /locus_tag="VBD85_000205"
     CDS             complement(27873..28787)
                     /locus_tag="VBD85_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7132784.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(29078..31093)
                     /gene="recG"
                     /locus_tag="VBD85_000206"
     CDS             complement(29078..31093)
                     /gene="recG"
                     /locus_tag="VBD85_000206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO7132785.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     ISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKSENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITCWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(31173..32711)
                     /locus_tag="VBD85_000207"
     CDS             complement(31173..32711)
                     /locus_tag="VBD85_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7132786.1"
                     /translation="MSKQKVMATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPDQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(32804..33904)
                     /locus_tag="VBD85_000208"
     CDS             complement(32804..33904)
                     /locus_tag="VBD85_000208"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7132787.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(33901..34260)
                     /locus_tag="VBD85_000209"
     CDS             complement(33901..34260)
                     /locus_tag="VBD85_000209"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO7132788.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(34285..35292)
                     /locus_tag="VBD85_000210"
     CDS             complement(34285..35292)
                     /locus_tag="VBD85_000210"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO7132789.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(35418..37946)
                     /gene="secA"
                     /locus_tag="VBD85_000211"
     CDS             complement(35418..37946)
                     /gene="secA"
                     /locus_tag="VBD85_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO7132790.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMAALSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGPELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(38055..39002)
                     /gene="manA"
                     /locus_tag="VBD85_000212"
     CDS             complement(38055..39002)
                     /gene="manA"
                     /locus_tag="VBD85_000212"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO7132791.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLHIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(39120..40001)
                     /locus_tag="VBD85_000213"
     CDS             complement(39120..40001)
                     /locus_tag="VBD85_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7132792.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(40069..41988)
                     /locus_tag="VBD85_000214"
     CDS             complement(40069..41988)
                     /locus_tag="VBD85_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO7132793.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRVMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTSSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVVPLV
                     KEGVVTTGDMIIDVK"
     gene            42173..43612
                     /locus_tag="VBD85_000215"
     CDS             42173..43612
                     /locus_tag="VBD85_000215"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO7132794.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENIVKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTNDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTVKGKVIIDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            43614..44576
                     /locus_tag="VBD85_000216"
     CDS             43614..44576
                     /locus_tag="VBD85_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7132795.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIVSSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(44663..45097)
                     /gene="nusB"
                     /locus_tag="VBD85_000217"
     CDS             complement(44663..45097)
                     /gene="nusB"
                     /locus_tag="VBD85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO7132796.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(45090..45479)
                     /locus_tag="VBD85_000218"
     CDS             complement(45090..45479)
                     /locus_tag="VBD85_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7132797.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(45568..46128)
                     /gene="efp"
                     /locus_tag="VBD85_000219"
     CDS             complement(45568..46128)
                     /gene="efp"
                     /locus_tag="VBD85_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO7132798.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(47056..47355)
                     /locus_tag="VBD85_000220"
     CDS             complement(47056..47355)
                     /locus_tag="VBD85_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO7132799.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT        15013 a         9457 c         7605 g        15521 t
ORIGIN      
        1 accgatattt tgtcaaaagc ttgcgaaata aataaacgtt taaatgactt ggctattttt
       61 ttcttagtaa taagcgaaga agtcataatt tctcctttat tttatattga attgctgatg
      121 atttattatg gtttagtgta acatgattta tgattattga aaaccctaac aaagcggaca
      181 aacaagaaaa tctgtccatt ccagaaaagg cttacaaatt ttataataaa agagaattaa
      241 acataaaagg agtttccaaa tgaaaaaaat tctcaatcaa ccaactgatg ttgttactga
      301 aatgctagat ggattagcat atgttcataa tgatttagta catcgcatag aaggttttga
      361 tatcattgct cgcaatgaag agaagtcagg aaaagtggct ctcatttctg gcggaggaag
      421 tggacatgag ccttcacatg ctggtttcgt aggcgaagga atgttatctg cagctgtgtg
      481 tggtgcagta tttacttctc caactcctga ccaagttctt gaagcaatta aagaagctga
      541 tgagggggct ggagttttca tggttatcaa aaattattca ggggatatca tgaactttga
      601 aatggcacaa gatatggctg aaatggaagg catagaagtt gcaagtgtgg tcgttgatga
      661 tgatattgca gtcgaagaca gtctttacac acaaggaaaa cgtggtgtag ctggtacgat
      721 actcgtccat aaaattctag gacatgctgc tcgtcatggc aaatcacttc aggaaatcaa
      781 agctattgct gatgaattag taccaaacat tcacactgtt ggacttgcac taagcggggc
      841 tactgttcct gaagtaggca agccaggatt tgttcttgca gaagatgaaa ttgaatttgg
      901 tatcggtatt catggtgaac caggttatcg taaagaaaaa atgcaacctt caaaggcttt
      961 agcgacagaa ttagttgata agttaattga atcgttcgat gctaaatctg gcgaaaaata
     1021 tggtgtcctt attaacggta tgggtgcaac cccacttatg gaacaatatg tctttgctaa
     1081 cgatgttgcc aaactccttg aggataaagg cattgaagtg aattacaaaa aacttggtaa
     1141 ctatatgact tcaattgata tggctggttt atcactaacc ttgattaaac tggaaaatca
     1201 agagtggtta gaagcactta atagtgacgt tacaaccatt gcttggtaat cattcctgtt
     1261 aattagaaca aaaggaagga actcttatgg aagtaaaaac tgcaatagaa tggatgcaca
     1321 cttttaacca aaaaattcaa tccaacaagg actatttatc cgaactagat actccaatag
     1381 gtgatggcga ccatggtggc aatatggcgc gtggtatgac tgctgttatt gaaaaccttg
     1441 ataacaatga atttagtagt gctgccgatg ttttcaaaac cgtgtccatg caacttttaa
     1501 gcaaagtagg cggagcatct ggaccactct atggttctgc atttatggga attacaaaag
     1561 ctgaacagag taaaagtacc atttcagagg cacttggcgc aggacttgaa atgattcaaa
     1621 aacgtggtaa agctgaactt aatgaaaaaa caatggttga tgtttggcac ggtgttattg
     1681 aagcgattga aaaaaatgaa ttgactgaag accgtatcga ttccttagta gatgctacaa
     1741 aagggatgaa agcaacaaaa ggacgcgctt cttatgttgg agaacgctca gtaggacaca
     1801 ttgatccagg atcattttct tcaggattat tatttaaagc attactagaa gttggaggtg
     1861 tctaatgtct gatattggta ttattgttgt ttctcactct aaaaatattg ctcaaggagt
     1921 tgttgatctt atttcagagg tagcaaagga tgtctcaata acttacgtgg gtggaacaga
     1981 agatggtgag attggtactt cttttgatca agttcaacag attgtagagc aaaatgataa
     2041 aaagacgtta ttagctttct ttgatttagg ttcagcaaaa atgaatttag agttagtagc
     2101 tgatttttca gaaaaaaata ttatcattaa ctctgtccca gtagtggagg gagcttacac
     2161 cgctgctgct cttctacagg caggtgctga cctggatagt attcaatcac aattagctga
     2221 attaactatc aataaataag gagatattat gactcaattt cttggggaat ttttaggaac
     2281 atttattttg gttcttcttg gggatggcgt agttgcagga aatgtgctca gtaaaaccaa
     2341 agaagaagga actggatgga ctgctattgt ttttggctgg ggaattgctt gtaccgttgc
     2401 tgtttatgtt tctggattat tcagtcccgc tcatctaaat ccagcagtca cattagcaat
     2461 ggcttctatt ggagcaattt cttggggaca agttatcccc tttatcattg cacagatgtt
     2521 aggagcaatg gttgcggcaa ctattttatg gttacactat tatcctcatt ggaaagaaac
     2581 aaaagatagt ggacttatcc ttgccagctt ctcaaccgga cctgctatcc gccatacacc
     2641 atcgaatctc cttggtgaaa ttatcggtac tgctattctt gtgattacaa ttatggcaat
     2701 tggtcctagt aaagttgcag caggtcttgg tccaattatc gttggtatag ttattttcgc
     2761 agtaggattc tctcttggtc caacaactgg ttatgctatt aaccctgcac gtgacctagg
     2821 gcctcgtctt atgcatgcta tcttacctat tgaaaataag ggtaactcag attggtcata
     2881 cgcttggatt ccagttgtag gtccaattat cggcggtgtc cttggggcta tcttatacaa
     2941 tattgctttg actttagttt aactaaaaaa ccagaaagtt aatactttct ggtttttcat
     3001 agttgcagta tgctttcaaa atagaaaaaa caccaaaata cttagtgttc tttctggaga
     3061 ttttatgaaa aaagtttagg atgtttatag tataaccgcc cttacttaac gtttccttaa
     3121 aagctcggtt attaacatta cttcctaaat tactatttat attctccaag aaatgactac
     3181 tattaagcta aaaagcgcta ttgacatagc gctttttagt ctagtattat ttagtttcag
     3241 tagtaactcc atcaaaatct tcttcatttt cttcagcttc ttctcttgtg gcacggataa
     3301 taccatcttt gtgatgagct ggactgtaaa ttgtatagag ttttaacatc tcagtttcag
     3361 aagtgttaat tacgttatgt tcagcacctg ccggaactat tatagcgtct ccatctgcaa
     3421 cttcatattc attaccatca atgacaacct taccatgacc agcttcaaaa cggaaaaact
     3481 gatcattttc tgcatgaatc tcgctaccaa tttctccacc aacaggcagt gtcattaaaa
     3541 cgagttgaca atgttctgcg gtatagagga cttggcggaa atttgtattt cctagtgtca
     3601 atttttcgat attcccatga aatcctttca taacgatacc tacctttcta tgcctacagt
     3661 ctactctttt tgctccttac tgtcaaaaat ttagataaga gtctgacatt tttaataata
     3721 tctactgttc cttgattcct atcatttcat tgcaaaccta aaatatagcc taatgacaat
     3781 agctatctat agctttagaa acaaataatg ctgttccctg gcctccccaa ttatcaatag
     3841 acttttggaa acgctcatct tggatgtaca ttattccaag acttgctaat aaatcgttag
     3901 tgcagttgta aaactgagct gtgatataat cttgtaaaat ctgaacttgt ttttgaactt
     3961 tctcatctgt cggagaaagt tctttcaact gtccaaagtc tttaaaaatc tgactcatag
     4021 cttgtgaaat cattgaaaaa tcattcttac tatggctatt ttcaaattct ttgtaactat
     4081 ctgtatcccc ccagcgtgtt ctagcttcct cttgaaatgc ttctaattct tcttgattat
     4141 aagctgtaaa atctgacata tttttacccc tctggataga ctttgcatgt tcaattactt
     4201 cttctagtcg ctgtttcttt agttccagcc acctaatttg atcaagaagt gcttgattac
     4261 gatcataatt tggactatcc attatctctt ttattttttt aagaggaaat tccagttcct
     4321 taaataacaa aatttcttgt aacttaatca gtgattcatc atcatagtaa cgataaccat
     4381 tctctcctac aaatgaagga gataacaaat ctatttcatc ataataatga agtgtgcgca
     4441 cactaatacc agaaatgtga cttatttctt taactgtttt cataataacc tctctgtaaa
     4501 gagtataatg tataacgtta agtaagggtc aagggaaaaa tattattttg tgatgattaa
     4561 tttataaagc ttaaatcaaa ggtcactttg actaacaggt tgataaaaag ggatacgtcc
     4621 ccatgtttgc tctgctatat tagcatttcc catctgataa acagaatctg ctcgttgcgt
     4681 caaactatcc cacgtttgtg aaccaatcct cgtcacgata tccacagact tcttcacaga
     4741 ctttaaatgc tgttgacctt tttgtgtaaa acctaagaca tgaatagcat ttggtatagg
     4801 actttccaca gcatttatca agatataggt taataaacgt cgaattcttg ctttggtata
     4861 ccgtttagtt gcgactttat caactaactc atccacagtc tccacataac gaatttgact
     4921 ttttatacga ttggctatct cttcattcac ttgataaatt tgtgtcaaat cagaatgcgt
     4981 cattatctga tatttgagta aactaaaata atcttgccaa actacttgtg gactattcaa
     5041 aaacaagtca ctgttaggca tacctttttc tacaaagaaa cgatcagatt gatgtttacg
     5101 aagacttgtt gcagaagcaa atatctctgt tttttctgat gaatgataac cagctccttg
     5161 acgttgtatc ggatttaatc gaataccatt ttggctagct gctttggtgt aggctaagcc
     5221 taaaatgtga tttggggttt gtcccgaaaa tgtaatccct gcaaattctt gccacatagc
     5281 ttgcattttt tgtggatagg aaagagaatc cgattgtttt ttcaaaaaag cttccatctc
     5341 ctctttttta ttcacataaa tatccccaat acgggcataa tcaagcattt cttcggtacc
     5401 gaagcataag ttatccacac ctaatcgtgc caggatagac acagctccac tagcgaaata
     5461 atccgctgac tgtaccgaaa ccagaaaagg gagttcaatc actaaatcag caccattttc
     5521 tagtgccatc tgggaacggg tccatttatc tacaatcgcc ggctcaccac gttgcataaa
     5581 attacccgac atggctatta cttttatgcc ttgagcttgt tctaaaaggt acttatgccc
     5641 attatgaaag ggattaaatt cggcaacaat tcctgtaact gtcatacttc tttctctttc
     5701 aattgattat ttaaagtaac agcatcaata atatctaggt gataagtttt acgaaggtat
     5761 atgagacgat ctgtttcttt ttcaaatggt tttaatgccg ataataaatc caaatgaagt
     5821 ttagggttca tcaataattc caaaataact ttagactttt tctgacttac ccgacgttca
     5881 ataatataaa gtatcccaaa tatcatcaga caagcgtata agaatattat catcttttta
     5941 cctccaaatt attacttatt tatgagctac aaaaaaccag cgagcagaag ttgcagttgg
     6001 ttttttatct tcaaaatctg catacacctt aacatcttta aaacctgcct gttcaagtaa
     6061 aatatcataa gtcaaaatat cataagtacg ctcttcatga acttcatcat gacgtgtaaa
     6121 tcgaccatct tcttcttgaa caaaaaatgt taattcatgg acaatagaat gaggtgcatc
     6181 atcttcataa gtatcccaca ccatagcaaa gtcatctgca ttttcatgat aagaataacc
     6241 aggaaatact ttatctgttt gataagtcga atgaacatca aaaataaaga cgccattttc
     6301 ttctaaggcc ttatagactt cgataaaaac atcaccaacc tcgacttcat cttgcatata
     6361 acaaatagag tctgaatagc acgtgatgag atcatattta ccaacattag ataaatctaa
     6421 catatttcct tcaataaact ggatactttg gtgagcagaa gttgcacgtt ttttggcaag
     6481 tttcagcata tcgccactaa gatcaagtcc tgtaacagca taaccagctt gtgcaaatcg
     6541 aacagactga attcccgtac cacatgctaa ttccaaaagt tttttcttac cttttggaaa
     6601 atgacgaagt gaaaaatcag tccatttagc atataaagta tcatccatga cagcatcata
     6661 aacagctgca aaagtttcat acgttgccat ataatagttc tcaaatcttt cttgatatta
     6721 catctctcaa aaagcagtcc caagactgct tagctttatt ctgtcataaa gacttcagca
     6781 tcaagcaatg gtgcttcatg ccaaagtttt tcaaggttat agtgctgtct ctcatcttca
     6841 gagaagatat gaaccacgac gctattgagg tcaagaagta cccaaccagc tttactatcg
     6901 ccttcaaggt ggctagcatc cccaccattt ccttttactt gttcacggat attgtctgcg
     6961 attgcttcta actgcctact attacttgca ctcataatca caaagtaatc tgcaacactc
     7021 gttacaggct gtaaatctaa aattacaata tcttctgctc gtttttcatc cgcagcctta
     7081 acgactagct gtagtaagtc tttttctgtc attttattcc tcgtttatta atttcaataa
     7141 gctatctttt gctaccattg tcgcaaaacg atgatgccaa atattctcgt aatgtttttt
     7201 aatagaatcc ccacttaaag ttggtgtatc aaatgttagg tgtccacctt gtggaataaa
     7261 gatatcataa ccatattcaa aagcaacttt tactgatgta tcaaaacagt attctgtttg
     7321 aagtcccatc atgattaatt gactaatatg cttactttgc aagtaatcat gtaaatgagt
     7381 atctttaaaa caactattgt aagttttatc aattattttg tgatgcgtca caagatgtcg
     7441 ctgttcccaa atttcccact gtggagaacc ttgaggcaat tccttatcat gatgccttat
     7501 ccatatcact tcacaattag aacctttggc acttaaaagt aaatcatcta aaagcactgc
     7561 aaaatttgtt aaatgctttg gtttcttatc cataatacct tgttggatat caattagtaa
     7621 aagtgcacaa gtcatacttt cctccaaaat gaccactgca tcgccaaacc tatcatgacc
     7681 attattcaat aaaagaatta gtcgagataa ggtatataag cattataggt ttcaatcgtt
     7741 ttaggataga taggctgagc cttacttgct aaaaacgcaa ctgttctagc agtctcataa
     7801 gctactgctt tattaaggtc aactttcgct aactcacgag cttcttcaac tcctggaaaa
     7861 tctcgattgt gttcaatgta atctgctaca taaacgattt tatccaatgt ggacatttgg
     7921 gctgaaccaa cagtatgttt tgcaatagca gctaaaatat cttgatcttt gatagctaga
     7981 tcctcttgta ttttataaat gccaacaaga ccatgccaaa tattattacc ccattttttt
     8041 aggtcgggat ctggttggta tttatcaatt aatctaagaa attcatcatc agatagttcc
     8101 ttagcataat catgtaataa agcagctaga cccgcttttt ccttgtcata accataccgt
     8161 tctgctaact ctattgcagc acgctctact cctaacacat gattaaaacg tttgtcggac
     8221 atcatatgac gcactttact caaaagttca gtccgatcta aacctgtgta atctttgtag
     8281 gtcatttgta aagaccttct ttcctgatat aatctaatac ctctctaggc agtaaatagt
     8341 tgggctgacg attactttta ataaattgac gaatcatact tgatgagata tccatcaatg
     8401 gcagatccac ccaaataacg ggatatgatg taccagcttt atattttgga cgttgaacac
     8461 caacaaactg caccatttta acaagttcat caatacgatg ccacttaggt agatattcca
     8521 ccatatctgc cccaataatg aaataataat caacatctgg atttttttca attagaagct
     8581 tcatcgtatc gtatgtatag ctgatacctt tacgttcaat ttcaattggt tcaatagata
     8641 aaccatcaat gccttctatt gctaattcaa gcatcttgag acgatgctgt tcatcaattg
     8701 tctctttttt atcaatatgt ggtggctgaa attcaggcat caacagaact tggtctaaac
     8761 acaattgctg acgtacttga tctgcaacaa caaggtgggc gttatgaaca gggttaaaat
     8821 ttcctcccat aattccaatt tgttttcggt tagtatctcg ctttttctct tctaattcaa
     8881 cttttgtaaa tggtgttagc aattcgagtg ccataattcc tccatgaaca agtagtatct
     8941 ctaaaaaaac aaacactgat tatcatgatc agctatcata cggaagtgta acttaggtaa
     9001 atcgtttttt agttctatac tctatttctt aatctattgt agtttagttt gattttactg
     9061 cttttacttt aactgaaatt ttacgatttt ctttgcgcgc tgactcttta taaagaatca
     9121 agatacgtcc tattttaagg acagtatcgc agccaatttc atcttccaga acttcagcga
     9181 catcgtggat atcttcatcg gtattttgta aaagggttac tttgattagc tcacgtgcat
     9241 cgagtgcatt acgtacgctt gttttgattt gatcatttaa accatttttc ccaatttgaa
     9301 taatgggttt catagaatgt gcttctgatt ttaagaaagc acgttgttta gatgttaaca
     9361 tattagtttc ctttttatct attataggat tatgactgta tcatcagcta aattagatga
     9421 aaaaccgtca tatgttgctg gttaatttag ttattaaatt aaagctttgc gtaaaacaac
     9481 tgccacgcct tctggagccc aagcagctac tactactgga ttttctgctt ctgatttcac
     9541 acgtatccaa ccaagacccg aataaacaat atccatttta tctttgattg taaattcatg
     9601 acgaactaat ttaggaaaat ctgaaacttc cttacctgtt ggtggtgtca ataattttcc
     9661 aacatgttta gtgtaaaatt catctgctcc aactagttta gtgcggtgta aattcaggtt
     9721 attatcaaaa tatgccgtaa aaccttgttt ttgaccagaa ataaaatcaa aacgtgctag
     9781 gccagccaag aataatgttt gttctgaatt aagctgataa gttttgggtt ttatttcttt
     9841 tttaggacta acatatttaa gattttttgc tgtcaaataa tgagccattt gatgacgatg
     9901 aataataccc ggggtatcaa aaatatagct accatcatca agaggtattt ctattttatc
     9961 taacgttgta cctgggaaac gtgaggtcgt aataacatca cgactaccag taatttcacg
    10021 aataatagcg ttaataagag ttgactttcc aacattagta accccaacaa cataaacatc
    10081 ttgaccatga cgatactttt caatcgtatc aatcaaatcc ttaatagcat gatgattttg
    10141 ggcacttgtc aagatgacat caacaggacg aagcccctct tcatgagctc tctcagttaa
    10201 ccattgtgtt acttttccat ctttaaccga tttgggaaga atatcttttt tatttcctac
    10261 taaaagaaca tcgtttcctg caacgaaacg actaagacca ggaataattg aaccattgaa
    10321 atcaaaaata tcaatgacat tcaccacaag ggcatcacta tctccaacct catgtagtaa
    10381 tttcaaaaat tcatcatcag tgatatgaac gtcagtaatc tcattatagt ggcgcaatct
    10441 gaaacaacgc tgacagtata attctcctgt ctctaaacct ttttcaagtg ctgcacgagg
    10501 tgtatagcca gcagcatctt tattttcagt ttgaatcctg gctccgcagc caatacaaaa
    10561 taactcttcc attaaattcc tttttgatat acgattttac cgtaattctc ttctattttt
    10621 ttccaaacgc gacgttcacg taagcgattg aacttggtat tccaagcatc agatttaaca
    10681 attggtttta ccaatacgga ctttattcct gcgcgatgac tagcacgaat atctgtcatc
    10741 aattgatctc caaccataat cacctcgtca cgatcaaagc cataacgctc aattgccatg
    10801 tttataccac gagtaaatgg tttcatcgca cgacttacaa aatccacacc aaaacgtgat
    10861 actgcacgtt ctacgcgagc atgattgtta ttagacacta ctacaactga aatatccgct
    10921 gttgtcatct catctaacca tgcacgcacc tcagcagtac catctggatt attccaagca
    10981 atgagtgtat tatctaaatc aactaaaacc gcacgaatgc catgacgtaa taaatcttct
    11041 gctcttaaat cataaactgc ctcaaccacg aaggtcggcc tataatcatc tatactcaat
    11101 ttcatctcca aaaaatatat tttactattc attctaccat aattcttaag agatagcgag
    11161 taaagaaaaa aggtctttaa agacctttaa attagggttt caaccccaaa agatactatc
    11221 ttactcgttt cattttcttc aatgcaatta aatctttgta agagattaga gttacagcaa
    11281 gaaaaataaa cagcatccca agtaaatcta ttggataaaa aatttctcct aaaacaagta
    11341 ctgtcaaaaa taccgaagaa actggctcta ccgatgctaa taagctaccc ttgacagcac
    11401 ctacaatact aaccccttta aggaaaaaag tgtaagcaaa aatagtccca ataccaataa
    11461 tgccaatata agcaagtata ctcatcacat taatttgtaa ggggtattgc cacgcttttg
    11521 tcaccaaact aaataaaatc ccacctataa acattcctga accaataaca atcgtacttc
    11581 cccattcatg aataagtttc acaggtaata aaatataaat agaataagta atggcggaac
    11641 caagccccca cattaatcct ttcggtgtga tagctaaact tcctaacttc ccatgcgttg
    11701 caatcatata ggttcctaaa attgccatag caattgctat tatttcaaaa gatgttggtc
    11761 tttgtctgtt taggatacaa acaatactca aaataagtat tggtgccata tattgcaaaa
    11821 ccgttgcagt tccagcattt gttaaatgaa tcgctctgag aaaagcatat tgatttaaaa
    11881 ctaaaccaaa tatagaaaaa agtaaaacct gctttaaaaa ttttggctgt ttccacgctg
    11941 ctactaaatg ctccttttgt tttgatctag ctaaagacaa gagaaagata cctgttatca
    12001 acaatcgtaa agaagtcaaa aggttaacgt gtactccgtg cgacatcaag tattgaccac
    12061 taattccaga aatgccccat gctagtccgg cagctaaggt catcattgtt cctttttctt
    12121 ttttagtcat ttttcccctc atttttattt cttaaacact gtagttaaag tcatctgata
    12181 aatatcttag cttcttaatt atttatccct aggtgttcgt gctaaattag cgaataaaaa
    12241 ccagccaaaa ggctgatttc tattcttctt ttaattttgc taattcttgc gctaacagct
    12301 tcagagcaac ttgtggatta gcttgtcctt ttgttgcttt cattagataa cctgtgaagg
    12361 ccttatcagc attgcgtttt cctgacttaa agtcaatcac agcagcttca ttatctgcaa
    12421 agacttgatg aataattggg attaatactt caggatctga aatttgaact aaaccagcct
    12481 ttttaacgaa ttcttcagct gaaccaccat ttttagctaa atgcacaaaa acttttttag
    12541 caattttgga tgaaattgta ccgtcagcaa tcaagccaat catctcaacc aaattctcag
    12601 gagtcagacc aatttcttcg attgattttg attcactgtt aagaaattgc gccacttctc
    12661 cttgtaacca attggaaact tgttttgcat cacccccaat agcaacagct ttttcaaaga
    12721 aatcagaagt tgcttttgta gctgttaatt ggctagcatc ataagatgat agaccaaaag
    12781 acgatacata tttcgcacga cgttcctgtg gaaattctgg taattctaaa cgaacttgat
    12841 caatccattc atccgagata tcaaatagtg gtaaatctgg ttcagggaaa tagcgataat
    12901 ctgatgagcc ttccttgaca cgcatcaaaa tagtctcacc tgttgtttca tcaaaacgac
    12961 gagtctcttg ctgaatttga ccaccagatc gtaaaacttg tgcttgacgt ttttcttcat
    13021 gtatcaaacc tttacgtaca ttattgaatg agttaaggtt cttaagttct gctttagtac
    13081 caaactcttc ttgaccataa ggacgaagtg aaatattggc atctacacgc atagaacctt
    13141 cttccatttt aacgtcagaa attccagtat attggataat ctctttgaga gctgtcaaat
    13201 aagcataagc ttcctcagga gaacgcatat cagcttctga tacaatttca atcagtggaa
    13261 caccttgtct attaaggtca acataggaat aaccatctgt cccatgtgta ttttttcctg
    13321 catcttcttc gaggtgggca cgctcaatac gaattttttt tcgtgtacca tcttctaatt
    13381 cgatttcaat ccaaccattg taaccaatag gctcatcaaa ctgtgaaatt tgataagctt
    13441 taggattatc tggatagaaa taatttttac gatcaaaatg catgttttga tggatatcca
    13501 tattcaaggc aagtgccgcc ttgattcctg catcaataac tcctttattc atgactggaa
    13561 ggacacctgg gaaagaccag tcaattacat ttgtattagc attttgttcc tgaccaaaat
    13621 gagcggaaga aggtgagaaa attttagagt tcgtatttaa ttcaacatgg acttcaagtc
    13681 caatgactgt ttcaaaattc attagttctc acctccaaaa attttaggtt gttgtttatg
    13741 ataatctgtc gttgcttcga aggcagcagc aacctgatat attgtttctt cactgaattt
    13801 cggaccaatc aactgcatac caaccggtag accttgatca aaaccagcag ggattgaaat
    13861 acctggtaaa ccagccaaat taactggtat tgttagaata tcagctaggt acattgcaac
    13921 tggatcgtga tttaaagaat ctaagtcaaa ggctgttgtt ggagctgttg gacctaaaat
    13981 caagtcataa tctgcgaaaa ctttttcaaa atcttgaata atcaaactac gaacttgacc
    14041 tgctttttta taataagcat cgtaataccc agaagataaa ctgaacgttc ccagcataat
    14101 gcgacgctta acttcgtctc caaaaccttc agatcgtgta ttgacataga tatcatccaa
    14161 gtttttataa ttttctgtac ggtatccata acgaatacca tcaaaacgtt gtaagtttga
    14221 agaagcttcc gatgaagcaa caatataata aacagcaaca ccgtatttag aatgcggtaa
    14281 actaacttcc tcaataactg cacctagttt ttccagatgt tttgcggcct taataatcgt
    14341 ttctttaact ccttgcgcta ttccttctcc caaatattct tttggtaaag caattttcat
    14401 tccttgaata tcttgaccaa ttttagcagt aaaatcgcct acagtacgct cagaagaagt
    14461 tgaatctctt acatcatgcc cagaaataac tgtcaaaagc tgtgcattct cttttactgt
    14521 ttgagacatc ggtccaatct ggtcaagcga actaccgaaa gcaaacaaac caaagcgtga
    14581 tacacgtccg taagttggct tcataccaac gattccatta aatgaagccg gttggcgaat
    14641 tgaaccacct gtatcagagc ccaatgataa acgtacttgt cctgaagcta ctgctgctgc
    14701 agaaccacct gatgatcctc ccggaacttt actatggtcc caagcgttat ttgtcttttt
    14761 aaaataagat gtttctgtag agcctcccat ggcaaattca tccatgtttg ctttaccaat
    14821 cacaatcata tctttagcat ataacttttc aacagccgta gcatcaaaga ttggctcata
    14881 gttatagagc attttagaag cagctgttgt aagaatcccc tttgttgaaa tattatcttt
    14941 aactgctaag ggaatacctg acataacatt atcagcatca attcctttat catcaatttc
    15001 tttggcttga gctattgcca tctcatcaga aatagtaata aaagatccaa ccgcttgttc
    15061 acgcgcatga atatcttcca atgtagcttt tgttaattca gttgctgaaa tttctttttt
    15121 aacgagaaaa tcatgcaact gatcaattga ttgattatta aacgacatta ggcatctcct
    15181 ccatcctcaa gaattgcagg tactttaata taataatctt gatgctgtgg tacattttta
    15241 aataagtcat cacgattatg gcctggttga gcaatatctt cacgcatcac ggttttgcga
    15301 tctgccatgg tcgttgttac tggtacgcct tctgtgtcaa cttcatttaa gagttcaatc
    15361 atatcaacaa tctttgacaa acttgacgca aattctttag tctcttgatc agaaaagcgc
    15421 aattttgaca gattggcaac atgacgcact tcttcttcag aaattttcat gagttctcct
    15481 tatctctgta tcattttcgt tttaagatag tctctactat tatagcatat taaactctca
    15541 atgttatcac attatcccag cttttcataa taaacgcttc ttacctatga taagaagcgt
    15601 ttactgttta tttatctatt gttatatcat ttctgctttg tttgatttta gcctgtgcag
    15661 ccgctaattt agcaatagga atcctaaacg gcgaacacga tacataatgt aaccccagtt
    15721 ggtaacagaa ctcaatagat gatggttctc caccatgctc tccacagata cctattttaa
    15781 ggtttggctt aacttctttt cctaacctca ctgcctgctc aataaacctt cctattcctt
    15841 tttgatctaa aacttggaaa gggtcttttt ttagaagtcc tttatcaacg tattctccta
    15901 agaattttcc agcatcatct cgtgaaaatc caaagctcat ttgtgttaaa tcattggtcc
    15961 caaaactaaa gaaatccgca aactttgcaa tatcatccgc tatcatacaa gctcttggtg
    16021 tttcaatcat tgtcccaata gggaatgata agttaatttt tttagattta ctctcctcaa
    16081 caattgtctc ttcaatcaga tcacgtatta tagagatttc ttcatgcgct gtcactaaag
    16141 gaatcataat ttctggcttt gcctcatatc cctctttcat tgcaagtata gcgccttgta
    16201 caagagctct cacctgcatt tggtaaattt ccggataggt aatcgcaagt ctacaaccac
    16261 gatgacctaa catagggttg aattcttcta atgtttgaat ccgtttttca atagctaaag
    16321 tagatatccc catttgtctt gccatagatt ctatagactc tttatcatgg ggtaagaatt
    16381 catgtaaagg agggtcaagc aaacgaatcg tacaagcttt acctttcaat actttaaaaa
    16441 tttgataaaa atcgtctctt tggaaagata aaagcctttc tagagccttc cttcggctca
    16501 atatttcatc cgctaaaatc atttctctaa cgactggaat acgttcatcg tcaaaaaaca
    16561 tatgttccgt gcgacaaaga cctattcctt ctgctccaaa gtccaaggcc ttttgagcat
    16621 ctctcggatt atctgcattt gaacacacca tcatatctcg attttcatct acccatttca
    16681 taaatatctc aaaatcacga tctacgctag tctctgccat cggaacttgt cctatataaa
    16741 catttcctgt agcaccatcg attgataaca tctcaccttc ttttatcgtt tgatgcccaa
    16801 tagaaatttc tcgtcttact tcatcaacca ataattgact acagcctgca acgcatggtt
    16861 tccccattcc tctggctact actgccgcat gggaagtcat tccgcctctt gctgttataa
    16921 tcccgtttgc actaaccatc ccctcgatat cttcaggtga tgtctcttgt ctgaccaata
    16981 gcactggatt tccctttttc atctctttaa caacatcttc tgcatggaaa taaactttcc
    17041 cataagcagc tccaggagaa gctggaagtc ctgtcgtcaa aataatagct ttctttaaac
    17101 ttttcaagtc aaaagaaggg tgtaataact gatctaattg tttgggctcg atacgtagta
    17161 tagcttcttc tttactaatt aggccttcat ttacttgatc tactgctatt ttgatagctg
    17221 ccttcgctgt acgcttaccg cttctcgtct gaagcatata aagattacca ttttcaattg
    17281 taaactccat atcttgcata tcgcgataat gagcttctaa cttttgggta acgcttacaa
    17341 actcttggta aataataggc atttgttctg ccaattttga tatggattgt ggtgttctga
    17401 tacctgcaac aacatcttct ccttgtgcat taatcaaata ctcaccaaat aaatttgctg
    17461 ctccagttga tgggtttcgt gtaaaagcaa ctcccgttcc gctgttattt cccatattcc
    17521 caaaaaccat agcttggata ttaacggctg tcccaagcgt ctccggaata tcatttaatt
    17581 tacgataaat cttagctctt ggattattcc atgatttaaa aactgcctca attgctaaca
    17641 aaagctgcct tttaggatct tgcggaaatt tttctcctgc ttctttttga tatagttcct
    17701 tgtaaaattc aacaagtctc tttaaatctg aaccagttag ttctgtgtca tcttgataac
    17761 atttatctgt cttaagtcga tctaaaatgg tatcaaattt atattttgga atgcctgtaa
    17821 ccacatctgc aaacatttgg ataaaacgac gataactgtc ataagcaaaa cgctcgtctt
    17881 ctgtcttttt cactaaacct tggacactac gatcatttaa ccctaaattt aaaactgtgt
    17941 ccatcatacc aggcattgaa aaaactgatc ctgaccgaac tgacaccaac aaaggatcat
    18001 caacacttcc taattgcttg ttctgttcaa cctccaattg tgccattgct tgatcaattt
    18061 gactaagaat actctctcgg atatggcaag cgttatcata ataatcatta caagcctgtg
    18121 ttgtaatagt aaaaccttgt ggtaccggta acccaatact cgtcatttca gctagattag
    18181 cccccttacc acctagcagt tccttcattt ccttacaacc ttcatcaaaa tgatacacaa
    18241 acttagtttc catcagcgtc tccttttaca gatttttagc tacttgtcca tttgttttta
    18301 agatttctaa aataatcgtt gctgtttctt cgattgcttt atcagataca ttaatgattg
    18361 ggcaacctaa atttttcata acttcttcag catacctcgt ctcctccaaa atccgttcta
    18421 aactggcata atttgccgta ctagaaagac ctaaggactt taatcgaact tttcggactt
    18481 gattaagatg atcaaccgaa ttagttagtc cgataatcct tctagaatca atcatacgta
    18541 attctttagg aacaggaacc tctggaacca aaggaatatt aataacttta atatttttat
    18601 ctgctaaaaa catacttaat ggtgttttag aagttcttga aattcctata ataactaaat
    18661 ctgcttgtaa aatacctcgc ggatctctcc catcatcata ttttactgca aattctattg
    18721 attctactct tttgaaataa tcattgtcta agcgtcgtaa aatccctggt tctcccaaag
    18781 gatctatacc cgatatcctt gatattcctt gaataacatt tgttaataag tcaacataag
    18841 cataatgttc actttcacat cgtttctgtg cataggaagc caaagcaaca tcaactaaac
    18901 taaacatcat aaatactgtc ttctggctag cctcttcaaa aacttgttct aagcgttcct
    18961 gactgttaat ataggaaaaa cgttgaaaat gccaatcatc atgtcctgga aattgtgaca
    19021 aacatgcttt cgcaatagct ttggctgttt cccctaaaga atctgaaata ataaatatag
    19081 tgagttgatc ttccatcagt ttcctcctaa taatctctta tttctaaaaa caaactagcg
    19141 aggattgttt tagataattt cccgatcact ttaaattttt cgggatattg cttatcatga
    19201 cgaacgacag ggagactatc cacttttctg ctcactaatt tatcagctgc cgcaaaaaga
    19261 ctttcatttt ctaaaacagt tgtcacgtgt ggcatacgtg tcatcaccat tcctattggc
    19321 attttagaaa gatctcctcc gccaatactg gtttttagta aatctttacg tgacacgact
    19381 ccacagagaa aatcatcatc atccaagata aaagcacaac cagcatcttc cataaaaata
    19441 tgtacaataa catcataaac tgaatctttt tgatgaactg tcagagggat ccccataatt
    19501 tctgaaactg tcatcttttc aaaatgactt gtccctattg aagcatgata ctgtcctaaa
    19561 taaaaataac caaccttagg ttttgcatct atgataccta gcatacttaa aaccgccaaa
    19621 tcagacctta gtgctgctct tgttactcct aatttctcag ctattagctc accagtaatg
    19681 ggctgaaact gtttgataat atcaataatt ctttcttgcc tttgatttaa ttgaataaga
    19741 tcaccttctt tatatattgt atactattta ttttatatgt tatattatat atcttttttt
    19801 actgaaatca agtaaaaaaa gatactattt taaaaataat catactatat atttgctttt
    19861 agacatatct tttgttaaac tttcttcgaa aacgttttcg aatataagta tcggaggttg
    19921 taatatgaca attaaaaatc tcactgttgc aggaagtggc gttctaggta gtcaaatcgc
    19981 ctttcaagca gcttacaaag gaatgtctgt caccatatat gacattaacg atgaagcttt
    20041 aaataaggga aaagaacgca tcaaaaaatt agctaaagtc tatcaatcag aaattgaaac
    20101 agcaaaagaa gcatatagtg ataaagctaa aagtatcaag tataataaaa acttattgcc
    20161 tagcttagac catatttttc tttcaaaagt tgccgattca ttagacttga ttgctgattt
    20221 accaaaccaa attacttttt caaaaaactt agaccaagct gtaagtgacg ccgatttagt
    20281 aatcgaagct gtcccagaaa cagttagtat aaaagaagat ttttataaac aattagctaa
    20341 agtggcacct tcaaagacaa tctttgcaac aaattcttca acacttgttc caagtcagtt
    20401 tgctgatata acaggacggc ctgacaaatt tttagcaatg catttcgcta acaatatttg
    20461 gcaaaataat attgttgaaa ttatgggaca taagggaact gacgacgagg ttatcaaaga
    20521 agctttgaca ttctcaaaag atattggtat ggtgccttta catattcata aagaacaacc
    20581 tggctatatt cttaattcaa tactagttcc attcttagag agtgcactag cattgtacta
    20641 tgataaggtt tctgattctg aaactattga taaaacctgg aagttaggaa caggagcacc
    20701 gatgggacct ttagaaattt tagatattat tggcatcgat accgcttata atatcatgaa
    20761 gaattattca gataccaaca gcgatcctaa tagtcttcat gctcaccttg caaaaatgct
    20821 taaagaagaa ttcattgata aaggacgtac cggtaaagct gctggacacg gcttttatga
    20881 ttacgactaa actataaaag aggttagata aaaatctaac ctcttttata attcaactaa
    20941 agaatagttg caccaagaac agttttaaaa tgatttaaag cccattgatg attactatca
    21001 tttaagctag ctacagctgc tgctgggacc tcaattttat aacctaagtt ataagcatca
    21061 attgctgtgt gtaatacaca aatatctgtc aatactcctg tcaatatcaa agtatctact
    21121 cgtctttcac gtaggcgaat atctaaatca gtgccagaaa aggcagaata atgacgctta
    21181 tctatccaaa aaacacgact atcttcctta atagtttcat atagagttcc caaaggacca
    21241 tataggtttc taccagaagt ccctttaata ttatgctcag gaaaaagatt gctctctgga
    21301 tgaaaaacat ctccaatatc atgattatca atcgcaaaaa aaatataatc ccctgaccgg
    21361 tatgctttct ctgtaacgtc agctatagct gaagcgatag attgcgctgg tttacctgcc
    21421 gttaatttcc catcatctgc cacaaaatca tatgtgtagt ctattgagat gagtgctttt
    21481 gtcatttttc ttcttatctc ctcttgattt caaaaaagga actgaaagct actcccagtc
    21541 ccatgctatg attaattata ttcttttaat ttgtcaaaaa taccttcgtt aataactttg
    21601 aggtatgttc ccttcatacc gagtgagcgg ctctcaatga ttcccgcaga ctccaattta
    21661 cgaagagcat ttacaataac ggaacgtgta attccaatac ggtctgcaat aacagatgcc
    21721 gttaggcgtc cttcaagtcc atctaattct cctaagatag cagctacagc tttcatttct
    21781 gaataagata gagtgttgat agccattgtc accgctgttt gtttacgaat attttcttct
    21841 agattttctg tttgaaggtt aagtaattga ataccaacaa cggtgcttgc aatttcaact
    21901 aaaatcaaat catcgtcgct aaattcttta tcattacgcc agataataaa tgttcccaag
    21961 cgcattccac caccataaat tggagcgatt gttgtaatac catcttggaa gttttccttt
    22021 gtctcaactg ggaaaattga caaatcatta tctactgata agttagcttc tgtatcataa
    22081 acacgactag cagatttaac atagtcatct gggaattgtt ttgtttcaaa aaattcttcc
    22141 acacggtcag tgtttgtttt atatttcatg gcatagccaa gtaagttacc accaccatta
    22201 ataatacatg cgttacagtc aataatatca gctaattgag ccgccattgt attatatggt
    22261 agttcggcat ctaagctatc tacggaacgc tgtaagatgg atgtaatttt tctagttttt
    22321 tctaataaat tcggcatttc cttcatcctt tgtatgtttt gcgttacaat tatatcaaag
    22381 cattttagct tttgcaaatc gatatcagta cattttaaga acttctattg ttaatttaat
    22441 gtttatctaa taaaaattca ttctagcgct tgtattggct taaaaagcga gccatttttt
    22501 cttgtagttc agtcaattca tcaacacgtg gtaagtaaac aattctaaaa tggtcagcgg
    22561 tattcatatt aaatcctcta ccgtgtgtta gtaatacttt ttcttgtttt aagaaattaa
    22621 gcacaaactc ttcatcatta tcaatacggt acatatctgt atcaatttta gggaaaagat
    22681 acagtccagc atttggtttt acagcagata aaccaggtat ttcattaatt gctttatgga
    22741 tataattacg ttgctcgtag atacgacctc ctggcaacaa catactatca atagattgct
    22801 gtccgcctaa cgatgtttga ataacctgtt gagcaagaac atttgaacac agcctcatgt
    22861 tagctaacat attcaaacct tcaatataac ctttaacatg ttgtcgcgga ccagacaata
    22921 ccatccaacc aacgcggaaa ccacagatgc gatgtgattt agataagcca gataaagtaa
    22981 ctgtaaaaat atcctctgca atagaagcaa ttgggatatg ctccattcca tccataacta
    23041 aacggtcata aacctcgtct gaaaaaataa tcaagtcatt ttgtctagcg atatccacaa
    23101 tctcttgcag aatttcacgc ggatagactg ctccagttgg attatttgga ttaatgagga
    23161 caattgcttt tgttttggat gtgatttttg atttgatatc atcaatatca ggataccaat
    23221 tagcttcctc atcacaaata taatgcacag catttccacc agcaaggcta acacaagctg
    23281 tccataacgg gtaatctggc attggaacta gaacctcgtc atcattatca agcaaagctt
    23341 gcattgacat agagattccc tcactaacac cattaacaat gtatatatca tccatgtcaa
    23401 catggatgtt ttgtaactgg taatactgca taacagcctt acgagctgaa aaaatacctt
    23461 tcgaatcaga gtaaccttct gattcacgcg cattcgtaat taaatcacga atgacttcat
    23521 ctggcgcttc gaagccaaag gcagctggat ttccagtatt taaccgcaat atcttttcac
    23581 catttgccct catgcggtcc gcctcttcta aaacagggcc tctaatatca taagctacat
    23641 gttccagctt cattgattta tcaaatatct tcataaaaat acctcgtatc ttccattata
    23701 aaacaaatat tctcaaaatt gaaactttta ctgcggttta tcaagcgatt aagttaatat
    23761 taagctattt cagaaaacta acataaaacg ctttcatttt tctagaaaaa acgttataat
    23821 atagctataa aatgaataac caacatatct aacttactca tcactcactc cttctgataa
    23881 gatggctagt tgagtgcaag aggttatctc ttttggttat atttagagga ggacaggctt
    23941 atgtcacaaa aatatgaacg catccttgtc gctattgatg gatcaacaga atctgaactt
    24001 gcttttgaaa aggcagttaa cgttgctctt cgcaatgatt ctgaattaat cttgacacat
    24061 gttatagata cacgtgcttt acaaagcttt gcgacatttg atacttatat ctatgaaaaa
    24121 ttagaaaaag aagcaaaaga tgtccttgag gaatatgaaa aacaagctcg tgaaaaagga
    24181 gcagataaag ttagacaagt cattgaattt ggtaatccca aaacattact tgcacatgat
    24241 attccagaaa aagaaaaagt agacttgatt atggttggtg caacaggtct taataccttt
    24301 gaacgtttcc ttatcggatc ttcctcagaa tacatccttc gtcacgctaa agtagattta
    24361 ctcattgtac gtgaccctaa taaaacaatg taaatttaaa tacgctcact ttctaaaata
    24421 tcctgagtta tattaactca ggatattttt attttcgatt taaggatttc tgaatttggc
    24481 tatctaattc acgtcgaatt gatggctcag gagcgaaatg ttcttcccag tcgtctggtt
    24541 ttaaaatttt atgagtaaca ggatcaaaat gagctttgcc atctggaaag attttcccca
    24601 tattggcctc atgtactgta tcaaaaagag gttttgggtc gactcccata agaacaaagg
    24661 agccataagt cagataaagt aaatctgtta aggcatccac ttctcccact aatggtgttt
    24721 ctggatggtc tttagaaata accttatctc ttgctttatc aatagctaag tgtaaattac
    24781 ggatagattg gtcaaatact ttttggttac cttgactagc agcatacaag aactcaacga
    24841 tttcttctac tttaaagtct gacctgaaac cagcttcctt actgtcatat aagcgtggcg
    24901 tttcaatagt ttcaccatcc attaatagat ggaaggattt tactttattg aaattctcgt
    24961 cacgactact gaatgttttt tcaatctcaa attggataag tccataatgg gctaatgcct
    25021 tagagatacc atcatcatta ttgtcagcag ttgtataacg tgcattttct ctcacttgct
    25081 gactagcgtt ccccatggca acaccaatgc caacttgaga taacatctca atatcattat
    25141 cagaatctcc aaatgctatc acttcggata gatcaaaacc atagcgttga ccaacacgtt
    25201 caattccctt aacttttgac tgcccttcag aaatgacatc cattgagtaa ggactgcttc
    25261 ttgttaattt aacacgagga aattgttttt gaattcgctc tgattcgccc tctgttgcaa
    25321 ctaaaacgac ctgataaata ggttctcgta agataaccat gaggctatca atattttgag
    25381 gtttaatccg acgaataaaa tgcttaaagc ttcgttcaat cgcttttgcc cattttctcg
    25441 gaacaaggct agatacaatc tgccccaaac ggctagttcc tagaccaata atacctgaac
    25501 cgagtaatcc tgaggctgtt cctaatgaaa tttctcttct tcttgctcca gcataacgaa
    25561 tcagacgata gacagttgtc ttagataatt gattggtata aataatttca cttctactat
    25621 agatatattg accattatag gttacagcaa aatctaaacc aaggttttct aaaaaaggtt
    25681 gtacaaaact aggacctcgc cccgttgcta aaccgactag tattccttgg tctttcaaat
    25741 ttcgaattgc tttaattgtt gacttttgta cattcttacg atcattaaga agtgtaccat
    25801 caatatcaaa aaatactgcc ttaattgcca ctgggtcaac atcccctctt ttttatgata
    25861 aaataaatta tatcacaact tgaaagaagt ataaaatcaa tgaaaaaaat ccttgttcta
    25921 cacacaggtg gaacgatttc catgaatgct aacgagaagg gacaggtcat gtccagcgcc
    25981 gataacccaa tgaaatacgt tgatttatca ttagatgacc ttgatttgac tgttgtagat
    26041 tttctaaatc ttcccagtcc tcaaatcacc cctcatcata tgcttgatat ctatcactat
    26101 ctgaagcaac atgccagtaa ttttgatggt gttgtcatta cgcatgggac tgacacttta
    26161 gaagaaacag catatttttt agacacaatg atactcccta aaatcccaat catcattacc
    26221 ggtgctatga gaagcacaaa tgaacttggt agcgatggcg tttataatta cctatcagcc
    26281 ctccgtgtcg ctaatagtac taaagctgcc gacaaggggg tgctagttgt catgaatgat
    26341 gagatacatg ctgctaaata tgtcacaaaa acacatacaa caaacgtttc gacattccaa
    26401 acccctacac atggccccct tgggattatt atgaaacaag acctgctttt ctttaaagca
    26461 actgaagaga gagttcgttt tgatttagat aaaattacag gaactgttcc tatcgtgaaa
    26521 gcatatgctg gcatgggtga tagtggtatc attagctttt taaattctca aaatattagc
    26581 ggattagtta ttgaagcact cggtgcaggg aatatgcccc caaaagctgc acaagaaatc
    26641 gaagaattaa ttgagcaagg tgtccctgtt gttctagtgt ctcgctgttt taatggtatt
    26701 gctgagccag tttacggata tgaaggtggc ggagcaaaat tacaagaatc tggtgtcatg
    26761 tttgttaaag aactaaacgc tcccaaagcc agactcaaat tactcattgc actcaatgca
    26821 ggtctcacag ggcaaaatct aaaagactat attgaaggtt gataaaaaac tctagaaata
    26881 tttctagagt tttttattaa ttttcagttt gtaatactct ctcaacactg ctaataggca
    26941 tttcttgatt cgttattaat tgaaatgctt ctgctccttg ataaatcagc atccctagac
    27001 cattataagc atgtttcact ccattttgtc taacaaatcg taacaatgct gtttctttag
    27061 gcttgtaaac caagtcgaca actactaaat tcggtgttaa aatttctgga tcgttaatta
    27121 aactataatt atctaatggc ctcattccta aactagtagc atcaatataa aaatgactag
    27181 ttctaatagc gtctttaaat gctgtcttat tttctagata atcaacgact acctttattt
    27241 gaaactgttt tttaatttta tctgataagt caatgacctt atcgtaattt gagctgttac
    27301 gattaaataa tctaatttcc gcaactccct ccatagctgc ttgaactgca actgctttac
    27361 ctgaaccacc aataccagct attgtaatta ttttattttt agcactgaaa ccttgagctg
    27421 ctaaagcttt aaaacaacca atgccatctg tcatatggcc tactaaacgt ccggttccac
    27481 cttgattaac gatagtattt acagcaccca ctaatttagc ttgaggagat aaatcatcta
    27541 gcaaagggat aacactctgt ttaaatggca ttgaaacatt aacaccacga atacccaatg
    27601 ccctgacacc tcgaacagct tctgttaatt taccctcttc tacttcaaat gtcagatagg
    27661 cataattcat gtttttttct tgaaaagagg tattccacat taacggggat agagagtggc
    27721 gtgcaggatt ggcaattaga ccagttagta aagtttcccc atttaatgaa ttcattgtat
    27781 aaccttcttt cttaacaatt tatttcagtt cattttacca aaaactgact caattaaaaa
    27841 gagagtatgt aatttatact ctctccctta gtttaaggca atatattacc tgcactacgg
    27901 taaatatcat accattcttt acgggttaaa gcgatatcat tagccttatc aatcgccttt
    27961 aaacgagatg gattcattga accaacaatc gcctgcattt tggcaggatg acgtaaaatc
    28021 catgcaatca caatagcttc gctgctaaca ttgtagtttt ttgccagttt agctatggtt
    28081 tcatttaatt ccttataatc cggatgatta acaaataaac cacgagataa atcaatttga
    28141 aatggagacc aagcttgaat ggtaactctt ttaagacgac aataatcaac aatgccatca
    28201 tcatgttcaa ttgaagcttt atttaacatg ttaacattta accctgcatc aaacattggt
    28261 gtatgtgctg gcgataactg aagttgattc actgctaagg gctcgtcaag gtaggattgt
    28321 aacaactcca tttgaaaccg gttttgattg ctaacaccaa agtgttttac tttaccttct
    28381 gccctcagtt tggtaaatgc ttctgctact tcttcaggct caactaatac atctggacga
    28441 tgaagaatga gaaaatctaa atattctgtc cctaatcttt ctaaaatacc atctactgat
    28501 gataaaatat actctttaga aaaatcaaag taaccttctc gtatcccaca tttagattga
    28561 ataatcattt tgtcacgatt gacatttaaa tgtttgattg catctcgaaa gcgtaattca
    28621 gatagccctc ccccataaat atcagcatga tcaaaaaaat taatacccaa atctaatgct
    28681 gttccaacta cttcttcagc ttgttttcct ttcaagtcac tcattctcat acaacctaaa
    28741 gcaattcgag ttgcttgaat gcctgtttgt ccaatgtttt gtaacatatg caccccattc
    28801 taatctgttt gtattaacag aaagattaat caattcatta taacaaaatt tctatgaaaa
    28861 ggcttactat tacaaatgca aaaagctgag taaatttaac tcaacttttt ttctgatact
    28921 cattattgac taaataagtc ggtaagaaaa atctattctg aaaagtacta ttataaatta
    28981 cagtaatcat tattgtcata acatacctcc acatctgtga taggaatatc atatgacagc
    29041 aagcttaaac tgagcttagc acagccttaa tagaaactta atcaaaatct gaatgttggc
    29101 gtaaattatc tagaataagt gcccattctg tattttcttt ccaattatta tctttgacaa
    29161 tatcactagc aacacgtctg gcttcttcta aaataggata atcctcaacg atatcagcta
    29221 cttgaaattc tggaattcca gactgacgag taccaaagat ttcaccagaa ccacgcattt
    29281 ttaaatccga ctcagctaaa acgaaaccat ctgtcgtttc tgtcatgatt gtcattcgtt
    29341 ttttcccaga atcagttttg ggattagcca ctaaaacagc gtatgattgt ttatatccac
    29401 gaccaacacg cccacgaagt tgatgtagct ggcttaatcc aaaacgatcg gcatccataa
    29461 taatcatgat cgttgcattt gggacattta cccctacttc aataactgtt gtagatacta
    29521 aaatatgact ttttttatcc ttgaaatctt gcattatagc atctttttca tcatttttca
    29581 tacgtccatg tacaagagca accttagcaa ttccttcaaa ataagtagat aattcagcat
    29641 gcaatgctac tgcattcttt aaatctaaag cttctgactc ttcaatcaac ggtgaaatga
    29701 catacacttg agcatctttt tgcaattcac cttttaccca ttccaacaca gtacctagct
    29761 gctcgtgttt cacccagcat gtaataatag gtttacgacc ggcaggtaat tcatcaataa
    29821 tagaaacatc catttctcca aaagctgtga ttgctagagt tcggggaatt ggggtggctg
    29881 tcatcattaa aacatcagga ttttctccct tttcacggaa aattctacgc tgtttaacac
    29941 caaaacgatg ttgctcgtct gtaattacaa gccccagttt atggtactgt accgagtcct
    30001 ggataagagc atgagttcct acaatcatat caaccgagcc atttgcaata gctgctaaaa
    30061 ccgtacgctt aacagctgct ttcatacccg aagttaatat agcgattgat aaatctggaa
    30121 ataactcttg cagactaatg tagtgttgtt cagctaggat ttccgttgga accatcaaag
    30181 ccgattgaaa acctgctgta taagctgcat acattgatag accagcaatg actgtctttc
    30241 ctgatcctac atctccttgc aataaacgat tcatatgagc tcccgatgac atatcagata
    30301 atatttcatc taaagagcgc ttttgagcat tcgttagaat aaaaggtaaa gaggaaattt
    30361 ttgtctccat agcatgttta ctataaagaa taggcaaacc atttgtttca gatttatttt
    30421 cggatttcaa aacttgaagg ttcatttgaa agtaaaataa ttcttcaaat ttaatccgac
    30481 ggagcgcttg cttatactct gtgatatctt ttgggaaatg catagctaaa caagcctgac
    30541 tacgacccat caatcggtat ttttccaata aagtagctgg taaattttcc ttcaattcca
    30601 aatgcgcact gatttcaaaa gctgacttaa tagctttaat caaagtagac tgtgaaattc
    30661 cctgagctac gcgataaaca ggttgcatgt catcttcaac ttgagctaaa accttcatcc
    30721 cagtaatagc tgatttagtt gcatcccatt taccaaagac agctacctct tgaccaagtt
    30781 ctattttatc agctaaatag ggttgattaa aaaaactaac atttaagaca gcttcccctt
    30841 gacgcaactt aaaacttaaa cggttacgtt taaaaccata atattgtaca ttagctggag
    30901 taacgactag gcctgtaatg actgcttttt caccatccac taaatcaaaa acagattttg
    30961 atttaaaatc ttcatagcga aacggataat aaagaagtaa atcttctact gtataaatat
    31021 ctaatttctg aaatttttct gctgattttg gtccaaatcc cttaagatta gaaattggtg
    31081 attgtaatag cataagattc tccttctaat atcttttatt atactaaaag atcaccaaaa
    31141 aggcgcctca agagcgcctt tattatgtct aattatttat ggccaataac ataagttaat
    31201 tgtgcagctt gtgctgaact aaaagtacgg taagagacaa taccacgtcc cattgcatta
    31261 gattctgaaa taagaactga accatctttt ttaacttctt caacaatagc tacgtgaccg
    31321 taagtgccat cagcaccagc ttgtcctggt gaaaatgata cagcatagcc tacttttggt
    31381 gaatgtgttg tttcaaaacc agccttatgt tgccaatctc caccattacc cataaatgga
    31441 tcaaatgtat aacctaactc tttagcgcgg ttaaagacat accatgtaca ttcaccccat
    31501 ggataagttg atgcaatata gctagctgtg ttaactgctg ttgataagct aaaaccactt
    31561 ggtaaatctg ctaaacttgc tgtcgataaa gctttcaagg cagcaggaat ttctggtgtc
    31621 cctccatgtt gatcaatcca gcggtctaaa tcttccataa tctgggcacg acgtttacca
    31681 gttccagagt tatctgtata ataaacacca ccctcagtaa ctgtattaag ttgtccagaa
    31741 gataatttct gtgctttcaa atcttggatt tcaaagctag agttgttgtt tttaataatt
    31801 gtattttttg tcaacaacat aattgcttct tcatcattat aagcactagc atggctagag
    31861 tcatgatcaa aggcaccata accaaacata ttagcacctg gcatcttagc cactccttga
    31921 gttcccaatg aactttcaag gacagcaata gctacaatag cacggacatc taaaccacta
    31981 tctttttccc attgtaataa cttagcacca ttgatacgat tagaatcata ttgaatccct
    32041 aaagatttta agtacccatc taattgttca gctttgatac cgtaacgatg tgacaataaa
    32101 tagtgagagt aggcatcctg accagaccag tgttctacgt agaatggcgt tgctgtcact
    32161 ggtgaaatcg gtgttgataa taagttatca tcaaaggata aagcaggaac agaagcattc
    32221 tgatttgtct gaacactttc tatctgagac ggagcgggag ttattttagg ctctactaat
    32281 ggtaaggtag gctcaggttt taattcagtc tttgttgatt gatctggctt actgtctgct
    32341 tgactactgt ttgtagtatt agtgctagga gtagatgctg ttgaagcaga atcagatgtt
    32401 ttagcatctt cgttggaaga agattgatgt gatgaacttg atgatgattc attgcttgat
    32461 gatgtctctg tcgtaccaat ctctgttttt tctgttgaga tacttccttc ggctgaaggt
    32521 gaagcagttt ctgaatctgt aggttgtgtt ttttcctgtt tctctgcttc agaagaactt
    32581 tctgttgatg ctgttgccat tgtcaggctt gtttctggat taatagtttc tgctaaggtc
    32641 actaaaggtg atgatagcga taagactaaa gtggataaca acaaagttgc cattactttt
    32701 tgttttgaca tatactaatg atttccttct ataaaaattg tcactgatta gtttaacata
    32761 tttttaaatt ttttaaaaca gttaactact gtgtcatttg tttttaataa tatctcggaa
    32821 tacgatcgct taaaagacaa agtacttcat aattaatcgt ctgtcttttt tgagcgacgt
    32881 ccgtagttgt aatgttacaa ctaccttgtt gaccaatcaa ggttacttta gttccgatag
    32941 tatatttttg aggtaaacgg atagtcattt gatccatcga gacacgccca ataatttcac
    33001 aaagttctcc atttactata actgaaaatc cttgcatatc tcgtgtccat ccatcagcgt
    33061 agccaatcgg tactgttcca acaaattcgt ctccagtaac ttgataggtt gcgccatagc
    33121 caacttgaga accatcgtgt agttgtttaa catgtactag ctcagactct aaacttaaag
    33181 cagggtttat agggtaaggt aagtctaaat cagttccact tggatttaag ccgtacataa
    33241 caacacccaa tctaacggca ttaaaaattg tctcactatg ccaaaggctt gtagcagaat
    33301 tactagcatg aacaaggtcc ggacaattat ctaatcctga aataaagtct ttaaaaaaag
    33361 taagttgttg attgaattta cagttatcgg cttcatcagc tgttgcaaaa tgggtgaaaa
    33421 tccccttgac agaggcacca ctttccccca attcagaaat tagcttattt ccttcatcta
    33481 actggcgaac acctatacgt cccattccac tatcaacttt gatatgtact tctagaccag
    33541 ataaatctac tgctgatgtc tgacataaac gtaaccactc caagctagca actgttagag
    33601 taatattttc atttttggct agtataacct gctcaggcat aacaacacct aataccaata
    33661 tcatttttac aattcctgct tgacgtagct caatagcctc atctaaattg gatacgcaaa
    33721 aaccatctac aatagactca atgtattttg aaacttcaac agcaccatgc ccataagcat
    33781 tagccttaac tacagcaaat gtttttttat cacttggaat gtgctcttgt acagatttaa
    33841 cgttatttgc aatcgcctct aaatctatca aggctctggt tggtctatga taacttgaaa
    33901 tcatttttct tcctccaaaa taacactagc ttgtgcataa ttaccactat gagatattga
    33961 aacaaaagat tttccattaa acggttcttt tgtaatcaaa ggcgctcctt tatcatccga
    34021 taaaatttcg atatcatgaa aattaacttt cccaattcct gttccaagtg ctttgctata
    34081 agcctctttt gctgcccatc gccctgttaa aaaagacatc tgacgcttgg gattggaaat
    34141 tcctttaaaa agaagcaatt cttgttcggt caaaacgcgt tctgcaaaac gttgattacg
    34201 ctcatatgct ttagtaatcg cctctatctc ttgtaaatca attccgtgtc caacaatcat
    34261 gtgtaactcc tataagttcg atacttacaa actattgtaa atatccctaa ttagcatttc
    34321 tgttttatcc caacctagac atggatctgt gatagactta ccaaaaacat ctggtttatc
    34381 ttgtcttcca tcttctaaat aagattctat caaaaaacca cgaacatagt ttctaatttt
    34441 agtatgccac tgtcggtctg caagtactga tttcacaatg cgaatttgtt ctaaaaattg
    34501 cttacctgaa ttgtcatgat ttgtatcaac aacaataaag ggattttgca aatcagtctc
    34561 ttcataatga gtaatagttt ctaaaaggtt ttcataatga taattaggga tacttcgata
    34621 gtcggcatta tgatatcctc tcaaaataac atgtgacagc aaattaccat ctgttctaac
    34681 ttgcttattt tgatagaata attcctgttg attttgtgct gcatagaccg cattaaacat
    34741 gactcttaaa ttaccagacg ttggattttt catacctact ggtgcatcta tacctgaaga
    34801 tataaaacgg tgtccctgat cttccactga acgcgcacca atagcatagt aagaaactaa
    34861 atcatccatc actgataaca tctctggata aagtaattca tcagctgtca ggagtccagt
    34921 ctctcgaatg atgtcatagt gcatcttacg tgcttgaaaa aaaactccta gtttgtcaga
    34981 atggtgaact aagcctttat aaccatcacc attcgttctt ggcttagcgg tataaactct
    35041 cataaccaaa aacattttat ctttaaaggc agcttccaat ttagctaaac gctttgcata
    35101 agttagcacc gcctttggat tatccgcaga acaaggacct actattacta gaaaccgctg
    35161 atcctcacct tttatgatat tttttaactc ttcttctttg gctaattttt tttggctaac
    35221 ttctagatct aagctagttt tctgcttcaa tatttcaata ttaattttat cacttaactg
    35281 atgaaatccc atgttttttc ctaatacaac ctaagtctat taataagctc tgctatacaa
    35341 tatagtcgtc atactattag acaagtaatg ataaaacagt cttctcagac tgttttattt
    35401 ttattttaaa atcattgtta ttgtaaacat ctcagaccat gacaattttt aaacttcatg
    35461 ccagagccac aaggacattt atcattacgt ttaatccctt ggtattctgg tgattcatta
    35521 ttcataggta catgttgtgc cgagatattt tgctctgctg ttgtcgtagc atgttgagaa
    35581 gcacgttcac gctcttgttc atgaatttga gctttcatca atgtacgtgt cacatcaaac
    35641 tcaatcgaac caatcatatc ttggaacata cggaaacctt ctgattgata ctcaacgatt
    35701 gggttatttt gagcataacc acgaagtcca acggagttac gtaattgatc taaggcatca
    35761 atatgctctg tccattgatt atccactacc atcaagatta agactttttg gaattcgata
    35821 atagcttctg gatcttttaa tttagcaatt tgttgctcat aaacagctaa ggcacgttca
    35881 taaagaagtt ctttaatttc agcctctttt agaccacgaa gttctgaaac tgtgatagct
    35941 tcttcatcta ataacgccga tcttgcaaaa ttaacaatag cttctgctgc agtattttta
    36001 tcgcttctgc tatgtgcatc aactgctctt ttaatcgtac gtttaatcat ccctttgagc
    36061 tctggtccta aatcacgctc agcagtaatc acttctctac ggttagcata aataatctca
    36121 cgttgttcac gcataacatc atcgtattgc aagacttgtt tacgtgtgtc atagttatta
    36181 ccttcaacgc ggcgttgagc tgattcaact tgtcgtgtca acatttttga tttaataact
    36241 gtatcatctt cagctaaatt cattcgctca agaaccactt taatacgatc tgtaccaaag
    36301 cgacgcatca aatcatcttc taacgaaaga tagaattgtg attcacctgg atctccttga
    36361 cgacccgaac gaccacgaag ctggttatca atacgacggc tttcatgacg ctctgtacca
    36421 ataacacata aaccaccaag ttcacgaacc ccttcaccaa gcttgatatc agtaccacga
    36481 ccagccatgt tggttgcgat tgtaacagca ccacgttgcc cagcattcat aatgatttga
    36541 gcttctttaa agtgattttt agcattcaaa acttcatgtg gtactccagc agcaactaat
    36601 tttctagaaa ttaagtctga cgtttcaaca gctactgtac caaccagtac aggttgtcct
    36661 tgttcataac gttctttgac atctgctacc accgctctaa atttagagtc aagagttgga
    36721 taaagtagat ctgaatgatc tatacgttga acaggacgat ttgttgggat aggaataacg
    36781 cgcatattat aaatttcacg aaactcttct tcctcagtct taccagtacc agtcattcct
    36841 gctaatttat ggtacatacg gaacatattt tggtaagtaa ttgaagcgct cgtttttgat
    36901 tcttcttgga ttggcactga ctcttttgct tcaatagctt ggtgtaagcc atctgagaaa
    36961 cgacgtcctt ccatcgtacg accggtaaat tggtccacaa ttaaaatctc ttgttcttca
    37021 ctaaccacat aatcaatatt taacaacata atatagttag ctcgaagagc gttatcaatg
    37081 tagtgagtta aggcaacatt ttcgagatca tacaagttat ttagatggaa atagttttca
    37141 gccttgtcaa tacctgtatc tgataaacca attgtttttg ttggaacatc aatgatataa
    37201 tcatcgctat taagcgtttt tacaaacatg tccgcacgtg tatacagttg gttcatctca
    37261 gagcttacag gacctgaaac aatcaatggc gtcctagctt catcgattaa tacagagtca
    37321 acctcatcaa ccaaagcata gttgagtggg cgttgcacca tatcttcttg gcgaacaacc
    37381 atattgtctc gaaggtagtc aaatccgact tctgcgttag ttgagtatgt gatatcacaa
    37441 ttgtaagctt cgcgtttttc aaatggtgac ttagctgcta agttaatccc tacagataat
    37501 ccaagccaac tataaacctc acccatttcg gtcgcatcac gtgttgataa atactcatta
    37561 acagtaataa catgaacacc taatcctgag atagcattta gataaacagg cattgtcgct
    37621 gttaaagttt ttccctcacc agtacgcatt tctggaatat caccatggtg aagaacgata
    37681 ccacccataa tttggacgtg gtatgggtat aaacctaata cacgtttaga agcttcacga
    37741 actaccgcaa acgcttccgg taacaattga tctaaagttt caccattttg ataacgttct
    37801 ttaaattctg gagtttttgc ttgtaaagct tcatctgata acgcagccat atgatctgcg
    37861 taagagtcaa ctttcttagc tattttatct aattttttta attcaccctt gtcattttca
    37921 ataacagtgc gtagaatatt tgccattcta attcctttcg atactcttat caccacattt
    37981 taccataaat aatgttgtct ttcaataaat ggtcgtttta atcgagatta aaaagagtgg
    38041 tatccccact ctcattattt tttaacatga cttgctataa tttctaactg tccatcaata
    38101 tcccatttga caacatcatt tggcaagata aagtggtcac cttttttaag tgtataaact
    38161 ttgttatcta ctgtaatgtg acccgctccg tctaagacac taaccaaaag gtagggtgca
    38221 aattgtttaa agttggtaac accagaaatt tcccatttat aaactgtaaa gaaatcattt
    38281 gaaactaata aagttgaaga tagatgtttt aacttcatat tggcaggaac agtattggca
    38341 ggtttaccaa tcgtgagaac atctattgat tgttcaatgt gtaaatcacg taattttcct
    38401 gaagcatctg gacgatcaaa atcataaaca cgataagtag tatcactaga ttgctgtgtt
    38461 tctaaaatta atatcccttt acctatagca tgcattgtcc cacttggaac gtagtaaaaa
    38521 tcccctgatt taacaggaat tcgggttaaa agatgttccc aatctccaga ttcaatcatt
    38581 tggcgaagtt cttctttagt tttagcatta tgaccataga taatttcaga tccttcatca
    38641 gcagaaataa tataccaaca ttctgtttta ccaagttcac cttcatgttt aagagcataa
    38701 gcatcatccg gatgaacctg tacactaagc cagtcattag catctaagat tttagttagt
    38761 aaaggaaaga cagtatcatc agggttgcca aataagcttt tttcaccttg atacagctta
    38821 tctaaaaaac aacctttaaa gcgaccattc ttaactcttg agacaccgtt aggatgagct
    38881 gaaatagccc aatattctcc tgttgtttca cttggaatat catagccaaa ttcatccctt
    38941 aactttgttc caccccagat tttatcgtgc atgcttgctt ctaaaaataa tggttccgac
    39001 ataagaatcc cctatctttt gtaaaattca agcctattat agcattttat ccatcatatc
    39061 gcttactttt tttaatcttt tatttgctaa ctaaactaag tagtggctta agggttttat
    39121 taaccttttg atattttctt tgctaaagca aaatttccta acgttgcaga accattttct
    39181 tctatagctg gtgtcacaat ataatcagac aaatcgggaa ctggtaaata cccatttaat
    39241 aaagttgcaa atgtctggcg aacacgtctt aacatgtgct cttgtgccat gacgcctcca
    39301 ccaaatacaa tgacttgtgg acgatataat acagttgctt gtattgcagc ctgagcaata
    39361 taataggctt gaacatccca aaccattgaa ttttcttcaa tcaattcacc acgaatacca
    39421 gtcctagctt ctagagttgg accagatgcc aaaccttcta aacagctatt atgaaacgga
    39481 cagatacctt taaaatcatt tgcttggtcc tgcggatgca tcgccatata agtatgacct
    39541 gcttcagtat gaccagttcc tcctataaat tcaccctttt gtatagctcc tgcaccaatt
    39601 cccgtaccaa tggtgtaata aacaagtgaa tcaatattgt tacgagctat aacttcacca
    39661 taggcagagc tattaacatc tgttgtgaaa taaaatggaa cattgaaatc tttagaaatt
    39721 aagccgagta aatcaacatt agcccaatgc aactttgggg tcgttgtgat ataaccgtat
    39781 gtctttgatt ttttatcaat atcaatggga ccaaaagaac caatagcaac tgcctctaac
    39841 ttcttttcaa atcttttaaa gaaatcaaca gtctttttaa tcgtttcttg aggcgtcgtt
    39901 gttggaaatt gcattttttc tacaactttt aattcctcat caccaactgc acaaacaaac
    39961 tttgtcccac ctgcttcaat actaccatat aactttgtca tcttttttcc tatctataat
    40021 ctctatctca aagacaaagg ttgggataat tatcccaacc tttaatcctc acttaacatc
    40081 aataatcatg tcacctgtag taacaacacc ctcttttacc aatggtacaa catcagcaaa
    40141 atcagctgta ttagtcacaa taatcatagc tgtattatcc agacctgctt ctgcaatctt
    40201 ggctgaatca aaggtgccaa gaatatcacc ttttttcact ttttggtctg ccttgacttt
    40261 agaagtaaac cctgtgccat tcatggtgac agtatcaata cctatatgaa tcaaaatttc
    40321 tgcaccttta tctgatttga taccgtaagc atgacctgtt tcaaaagcaa tttggacaaa
    40381 accatcaaca ggtgagtaaa ttgtattacc tcttggttta atagcaaggc catctcccat
    40441 aatacctgat gaaaaaactg ggtcacttac tgtatcaaga gcaataacct gtccatcaag
    40501 agggctaaca atcgtttctg cccttaattg gctagtttga gtaattgttg gatcgtcatc
    40561 tgtttcttca accaaaggac taacttcagc tactgctttt tcatctttat cttcatatcc
    40621 aaacatataa gttaatacaa aagccaaaga cgtagttcca attaccataa taaggtactt
    40681 aacaatttga ccatttaaat aaagtaatgt tcctgggata atggtaatac caaaaccagt
    40741 tcctgctaat ttcaatattg aagctaacca gccacctgct gctcctgcaa ttaatcccat
    40801 aataaatggc ttaccaaaac gtaagtttac tccaaatata gccggttctg taataccaag
    40861 tccagctgaa agcgctgccg gaaaagcaag ggcttttaat tttttatttt ttgttttaac
    40921 accaactgcc aaagtggcac ctgcttgtgc tgtcatagca gctgtaataa tagcattaaa
    40981 tggatctttc ccatcagcag caattaactg agcttcaagt aaattaaaga tatgatgaac
    41041 acctgtaaca acgattatct gatgaacacc acctaaaata agacctgaca ggccaagagg
    41101 taaattaagg acaaattttg tccctgctaa aacatagttt tcaactgaat ggaaaatagg
    41161 accaatgaca aaaagtgcta aaattgacat aactgtaaaa gttaggaacg gcactaatag
    41221 taaatcaaga acatctggga ttttcttatg caaccatttt tcaagtttag caccaagtaa
    41281 acctacaaag aatgccggca atactgaatt ttgataacca actactggaa taaaaccgaa
    41341 aaattttatg ggatgggcat cacctgaggc cactgcccaa gcgtttggaa gggcagagtt
    41401 gaccatcatc aatcctaata cgagaccgat aacaggattt ccaccaaaaa ctctaaaagc
    41461 tgaccaagaa atcaaggcag gaaaaaaagc aaaggccgtg tcagtcaaaa cgacagtata
    41521 ggtataaaaa ttagaacttg aaaatgcttt tgaagtcgtc ccaaataaag ctaaaactgt
    41581 atcattatta atcgcaccgc gaatccccat aaataaacca gtagcaacaa tggctggcaa
    41641 caatggaaca aaaacatcac cgaaagtacg aatagctcgt tggaaagcat tcccttgttt
    41701 agctgcttct gccttttgat cagaggttga cgaagttggc aaaccttgag ccactacttc
    41761 atcataaatc ttattaacag taccagttcc aaaaataatt tgatattgac ctgaattgaa
    41821 aaatgctccc tgaacttttt cgatattttc aacagtattt ttatcaatga ctgtctcatc
    41881 ttttaccata acgcgaagac gagtggcaca gtgtgctacg ctacgcacat tactagcacc
    41941 accaatcgca ttaatgactt gtttagcaat ttctgtattt gaagccatct ataaatctcc
    42001 tttgatattt tgaaagcgtt tcattttatt tactctaata ttctagcacg tctcataaat
    42061 atatgtcaag cgtttgacat aaaataatat ttttcgatat aaagtgttga tttcaaaaga
    42121 gaaaacgttt actatatagt tattagatat taagacagtc aaggagtatc aaatgaattt
    42181 acccactgaa atccgctatc gtccttatga tgaatggact gaagaagata aggaaaatat
    42241 tgtaaagaat gtctcaaaat ccccttggag agcaacttat cacttagaag ctaaaacagg
    42301 gcttttaaat gacccaaatg gcttctctta ctttaatgga aaattccatc ttttttacca
    42361 aaattggccc tttggagctg cacatggttt aaaacaatgg gtacatacag aatcagatga
    42421 tttagttcat ttcaaagaaa caggaatcaa gcttaaacct gaccatgtca atgatagcca
    42481 tggtgcatac tccggttctg cattagccat tgacgataag ctttttcttt tttatactgg
    42541 taatgtccgt gacatgaaat ggaaccgtga cccccgccaa attggagcat ggatgacaaa
    42601 tgatggcaag attacaaaat ttgataaagt ccttatttca caaccaaatg atgtcactga
    42661 gcattttcgt gaccctcaga ttttcaatta tgataatcaa ttctatgctg ttatcggggc
    42721 gcaaaatagt aaaaaatgcg gattcattaa attatataaa gcacttaata atgacatcca
    42781 tcattgggag tttgtgggag atttagattt tggtggtact ggatctgagt atatgattga
    42841 atgtccaaat attatttttg tcaaaggaaa acctgtatta ctttatagtc ctcaaggact
    42901 agataaaaac gaattggact accaaaacat ttatccaaat acctacaaaa tcggacaata
    42961 tttcgatgct aactcatcta agatagttga accatcccct atctataatt tagattatgg
    43021 ttttgaagcc tatgctactc aaggatttaa cacttctgat ggtcgtgcct ttatcgtgag
    43081 ttggataggc ttgccagata ttgattatcc tagcgatcaa tttgattatc aaggagctat
    43141 gagtttagtt aaagagttaa gcataaaaaa tgggaacctt tatcaatatc ccgttcctgc
    43201 tatgaaaaat cttcgtcaac atcaagcaga atttaaaaca caacttcaaa ctaacaacac
    43261 ctatgaatta gaattactgg ttcctagaaa tgatctttct tcatttgttc tctttgctaa
    43321 tccgaaagga caaggactat ccattactat tgatactgta aaggggaaag ttatcattga
    43381 ccgctcacaa gccggacagc agtacgccac agaatttggt actagtcgcc agtgtgacat
    43441 tccaaaagat gctactagta tcaatatttt tatcgataag tccatctttg aaatctttat
    43501 aaataaagga gaaaaagtat ttacgggacg agtctttcca gatgcagaac aatcagggat
    43561 tcaactaaaa gaagggcacg ttcatggtaa atattttgaa ttaaaatatt aatatggttg
    43621 caaaattaac agatgttgct gcacttgccg gtgttagccc aacgactgta tcacgcgtta
    43681 ttaacaaaaa aggctattta tctcaaaaaa cagtcaccaa agtcaatgaa gccatgagaa
    43741 cccttggtta taaacctaat aatttagcac gtagcttaca gggaaagtcc gctaaattaa
    43801 ttggactcat tttccccaac atccgtaaca ttttttacgc tgaattaatt gaacaccttg
    43861 aaattgagtt gtttaagcat ggttataaaa ctatcttatg taatagtgaa aaggatccta
    43921 tcaaagaaaa agaatacctc gaaatgctag gagcaaatca agtagacggt atcatttcaa
    43981 gtagtcacaa cctaggaatt gatgattacg agaaagttga ggctcccata gtagcattcg
    44041 atagaaattt agctccacac atccctatcg tctcatctga taatttcttt ggaggaaaaa
    44101 tggctgctca aacccttaaa aaacatggct gtcaaaagat gattatgatt acagggaatg
    44161 ataattcaga ttctcctact ggacttaggc ggctaggttt ttcctatgaa tcaaaggaat
    44221 ccaaagttat cacagtcaca aatgggctat ctaacatgag acgtgagatg gaattaaaat
    44281 caattatctc gactcacaaa ccagatggta tttttacatc agatgactta actgctttat
    44341 tagtaatcaa acttatcagc caactagggt tgtccattcc agaagatatc aaagtgattg
    44401 gatatgatgg tacaagtttc attcaagatt acgtcccaca tctaaccact attaaacaac
    44461 ctattcgaga aattgcgcaa ttgatggttg agatactcct agctaagatt gaggggcaaa
    44521 agacaaataa ggattatatt ttaccagtca gtctcatccc tggtagtagc gtttaggaaa
    44581 tcatatttat gaaataattt ttagtaccga tagttatttt tcaaagcaaa ttaaaaatgt
    44641 ggaagaagat tcttccacat ttttaatttt catttgttat aaattgactt aataaaccat
    44701 tgacaaattt tgctgacgtc tcatctgaat attttttagc aatctcgata atctcattta
    44761 gagctacacg atcaggcgtt tcatcaaaat atttaatttc atagaggcct aagcgtaaga
    44821 gagatttatc aaccaatgtt agacgttcta agctccatcc tgattttaag tgactagaaa
    44881 tcaaagtatc taattcatct ttgtgatcta caacaccatt aacaagatta agtaaaaaga
    44941 ttggaacctc taaaactttg tcttcagata ctgttttatc ataaccataa gcaaagtgag
    45001 ccgcatcaat aaactctcca ccagtttcta agctaaatag tgtttgaaaa gcacgttcac
    45061 gtaaatcacg tcttgaatct ttaaatacac tagtcatcta agaaatcctc atcaaacaat
    45121 gacttcaagt ctggttttgg tgttttctct ggaacaatac cttcaacatg gatattgacc
    45181 gaagaaattt ttacttctgc catgtcatat acagctgttt tgacagcttg ttgaatagca
    45241 attgaaactg ctggtacatt tacaccatac tgcaagtaaa cataaatatc tgcagctact
    45301 gtatcatctt cttcattttt aaggtagaca cctctaccta gagatttctt actaaggcta
    45361 tccgttaccg ctttgttacg taacgaatga acaccatcaa ctttagttgc agcaatacct
    45421 gtaattacct caagtacacg tggtgaaatg acaatttctc caagattttc agttgtcata
    45481 gcttatcctt tcttttagtg tttactaagg tttttcctta atagagtaaa ttcatcatga
    45541 atctacttaa cacttttttg agagtcatta ggcgcgtgaa acgtaagttc cttctgctgt
    45601 attgattact aacttttgtc cagcttcaat aaaatctgga acgttaacaa caagtcctgt
    45661 ttccatagtt gcaggttttc ctgaaccagt tacagtagca cctttgattg atggttgagt
    45721 ttcagcaact gtcaattcaa cagttgttgg aatttgaaca ccaataactt ctgttccata
    45781 gaattgaatt tttacttctg agttttcaag aatgtagagt aattcatttt caatgttaac
    45841 agttggaatt tcatattgat cgtaagtttc attattcatg aagtaagctg tatcatccat
    45901 cttataaagg tattgagcag gtacagtttc aataatagct tgctcaaatt tttcttcagg
    45961 acggtaactt gtatcaaaag ttgaaccagt acgaacatca cgcaatttca tacgcatgat
    46021 tgtgttacct ttacctggtt tgtggtggct agcttcaaga acacggatta atttcccatc
    46081 agccgtttca aaagtcatgc ctgctttaag cttacttgct tcaatcattt attaatacct
    46141 ctttgtaaaa attatttatc aactcatttt atcacaaata acctgctttc tcaaatagtt
    46201 tattgatttg gtatgtgtct gatttcttga taaataaata aaatcaccaa gagggcgtaa
    46261 ataagactga agcacttgac actttcctct tacttcagtt ttgataacag ttttaatatt
    46321 ataccgatat cttgactaag attgaaatac tgtttaggtg tttttagtga ctttttagta
    46381 aattttgata aaggtagtaa actatagttt tcttattgaa acagataaaa taaatcacct
    46441 acagagctat tcgtatgaaa atgtagttat tctcgatata agttttagat acgaacttaa
    46501 tttgctctat tgaagctggt aaaggaaatc ggggtggtgt tagtggacgt gcgctaagca
    46561 ttggtactga gcttggtgat accaatcttg cttactacat caggtagctc tctgttttag
    46621 tgccatatgc aattggaata agagctgtca ttggtattga aggagcccta gtccactgat
    46681 cattgaatta ctaatttagg aggtggaatg atgaatttta atatgtctca ttatagaaag
    46741 ttacaactat gtcgttttct aatcgtacag atacctgcgc tggctttatt agctattctt
    46801 ttatacggga ttttcctaaa gtacagtcta ttttcaaaag tattttttct tatcttttag
    46861 gggttgaaat agttatcaat tttgggaagt acataccagt ttacaccaac cacaatttgt
    46921 cgagtaaaga attgtaattt agtaaagccc ttttcgtttt cttctgataa gtgtatcatg
    46981 acatctatca atagcatatt cacactagta gcatattgat ttgtttttga gtagaattta
    47041 ctttttagat tatttctatt ctacactagt ctttcccttt ggaagattct ttccctctgt
    47101 atttttccat ttttcaatga gacgagaaac tctttcttcc tcctcatcat ggtaaccttc
    47161 aaaaacaacc tcatcaatat cttcttgatt aaaatagtaa acttgttcaa gatttaaacc
    47221 ctcaggaaaa attccaccta ggtaatcaaa ataaacttct tgtccttctt gctcaacaat
    47281 tgcacctcta tttacaataa ctaatttttg attgccatca ataagataga caacactccc
    47341 tactggtaat aacatacttc agttctcccc aatataataa tttttcccat ttaacgatgg
    47401 ttgaaactca atgaacttta taaaaaataa actgtttgaa aaaataaatc caaataaaaa
    47461 taagtttgaa aagttttcgt cccacaatgc cattccccat agaacgatat aagaaattat
    47521 gattgataat attactataa atgggctctt ccgtttttga ttattatatt tcaaaaaact
    47581 agatttatat ttggaa
//
LOCUS       DATRSD010000005        40610 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRSD010000005 DATRSD010000000
VERSION     DATRSD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40610
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.005"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..1138)
                     /locus_tag="VBD85_000221"
     CDS             complement(<1..1138)
                     /locus_tag="VBD85_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7132800.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGKPKGAPNTYLIDDLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFN"
     gene            complement(1157..2368)
                     /locus_tag="VBD85_000222"
     CDS             complement(1157..2368)
                     /locus_tag="VBD85_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7132801.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQGTI
                     DRLNKEIDFLKEAQKAYDRDIIPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(2381..3616)
                     /locus_tag="VBD85_000223"
     CDS             complement(2381..3616)
                     /locus_tag="VBD85_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7132802.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIMDYSNKELVNFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQKDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNDIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            complement(3601..4413)
                     /gene="yidA"
                     /locus_tag="VBD85_000224"
     CDS             complement(3601..4413)
                     /gene="yidA"
                     /locus_tag="VBD85_000224"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO7132803.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKGVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEVTLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(4484..5800)
                     /locus_tag="VBD85_000225"
     CDS             complement(4484..5800)
                     /locus_tag="VBD85_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7132804.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEESDKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            5864..6262
                     /locus_tag="VBD85_000226"
     CDS             5864..6262
                     /locus_tag="VBD85_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO7132805.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGVQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGDCFANYELRIQWY"
     gene            6606..9290
                     /locus_tag="VBD85_000227"
     CDS             6606..9290
                     /locus_tag="VBD85_000227"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO7132806.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(9335..10195)
                     /locus_tag="VBD85_000228"
     CDS             complement(9335..10195)
                     /locus_tag="VBD85_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7132807.1"
                     /translation="MTKLAIMSDLHIDLNHFSTFETKTLINLLKKEKIDHLHIAGDIS
                     NHFTKDTLPFINNLKKHIKLSYNLGNHDMLDLTETEIQRLDFQTYRFDKKMLLAFHGW
                     YDYSFSNNRDIKDVEKLKKTFWFDRRLKRPNNDVTIQASILKRLDEILAKVDSSNIII
                     AMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQKYHIKDVVFGHAHRSFGDVKIGET
                     TYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLSKRYNAVKHLDSFENYRKKYFEDE
                     LRNSMTIFDC"
     gene            10347..12278
                     /locus_tag="VBD85_000229"
     CDS             10347..12278
                     /locus_tag="VBD85_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEO7132808.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSAS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLSGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFVPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKEVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIIWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            12368..13492
                     /gene="queG"
                     /locus_tag="VBD85_000230"
     CDS             12368..13492
                     /gene="queG"
                     /locus_tag="VBD85_000230"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO7132809.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTSYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            13561..14659
                     /gene="prfB"
                     /locus_tag="VBD85_000231"
     CDS             join(13561..13632,13634..14659)
                     /gene="prfB"
                     /locus_tag="VBD85_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO7132810.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            14678..15370
                     /gene="ftsE"
                     /locus_tag="VBD85_000232"
     CDS             14678..15370
                     /gene="ftsE"
                     /locus_tag="VBD85_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO7132811.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            15354..16283
                     /locus_tag="VBD85_000233"
     CDS             15354..16283
                     /locus_tag="VBD85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7132812.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(16336..17046)
                     /locus_tag="VBD85_000234"
     CDS             complement(16336..17046)
                     /locus_tag="VBD85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7132813.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGDNTYILKTPLNLPV
                     LSPHKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSRKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(17043..17741)
                     /locus_tag="VBD85_000235"
     CDS             complement(17043..17741)
                     /locus_tag="VBD85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7132814.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            17909..18673
                     /locus_tag="VBD85_000236"
     CDS             17909..18673
                     /locus_tag="VBD85_000236"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO7132815.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            18781..21288
                     /locus_tag="VBD85_000237"
     CDS             18781..21288
                     /locus_tag="VBD85_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO7132816.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGCELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQVVFADLVKAHFN
                     DQTTTFLEAQPGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSSIPLLQKRLLESISYELSH
                     AVELFYRHNSFEFSETWLKRLKNSINALEVVGLDELQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSKFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DTPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            21374..22567
                     /locus_tag="VBD85_000238"
     CDS             21374..22567
                     /locus_tag="VBD85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7132817.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLETLKEAVRRLHVFMG
                     SNEIN"
     gene            22588..23934
                     /gene="asnS"
                     /locus_tag="VBD85_000239"
     CDS             22588..23934
                     /gene="asnS"
                     /locus_tag="VBD85_000239"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO7132818.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(23974..24532)
                     /locus_tag="VBD85_000240"
                     /pseudo
     CDS             complement(23974..24532)
                     /locus_tag="VBD85_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
     gene            complement(24529..25512)
                     /locus_tag="VBD85_000241"
     CDS             complement(24529..25512)
                     /locus_tag="VBD85_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO7132819.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRHDIPVYVGDNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFISMGGSFKSHGN
                     CSPVAEYNYWCDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSSF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(25656..25700)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(25758..26171)
                     /locus_tag="VBD85_000242"
     CDS             complement(25758..26171)
                     /locus_tag="VBD85_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO7132820.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVTCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            26325..27215
                     /gene="rapZ"
                     /locus_tag="VBD85_000243"
     CDS             26325..27215
                     /gene="rapZ"
                     /locus_tag="VBD85_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO7132821.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISKEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQNEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            27212..28186
                     /locus_tag="VBD85_000244"
     CDS             27212..28186
                     /locus_tag="VBD85_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO7132822.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            28183..29094
                     /gene="whiA"
                     /locus_tag="VBD85_000245"
     CDS             28183..29094
                     /gene="whiA"
                     /locus_tag="VBD85_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO7132823.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            29109..30506
                     /locus_tag="VBD85_000246"
     CDS             29109..30506
                     /locus_tag="VBD85_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO7132824.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMISKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPNYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGVDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            30648..32168
                     /locus_tag="VBD85_000247"
     CDS             30648..32168
                     /locus_tag="VBD85_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO7132825.1"
                     /translation="MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKIKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQAFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(32281..32541)
                     /locus_tag="VBD85_000248"
     CDS             complement(32281..32541)
                     /locus_tag="VBD85_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO7132826.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(32650..33585)
                     /locus_tag="VBD85_000249"
     CDS             complement(32650..33585)
                     /locus_tag="VBD85_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO7132827.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVVVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLKIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGVPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEILSL"
     gene            33851..34873
                     /locus_tag="VBD85_000250"
     CDS             33851..34873
                     /locus_tag="VBD85_000250"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO7132828.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPSSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVLAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKDIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRDLIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            34932..35375
                     /locus_tag="VBD85_000251"
     CDS             34932..35375
                     /locus_tag="VBD85_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO7132829.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            35452..35727
                     /locus_tag="VBD85_000252"
     CDS             35452..35727
                     /locus_tag="VBD85_000252"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO7132830.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            35720..36916
                     /locus_tag="VBD85_000253"
     CDS             35720..36916
                     /locus_tag="VBD85_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO7132831.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYVLPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            37496..37843
                     /gene="rplS"
                     /locus_tag="VBD85_000254"
     CDS             37496..37843
                     /gene="rplS"
                     /locus_tag="VBD85_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO7132832.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            37954..38025
                     /locus_tag="VBD85_000255"
     tRNA            37954..38025
                     /locus_tag="VBD85_000255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37987..37989,aa:Arg,seq:cct)
     gene            complement(37988..38602)
                     /locus_tag="VBD85_000256"
     CDS             complement(37988..38602)
                     /locus_tag="VBD85_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7132833.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(38605..38871)
                     /locus_tag="VBD85_000257"
     CDS             complement(38605..38871)
                     /locus_tag="VBD85_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO7132834.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            38871..39275
                     /locus_tag="VBD85_000258"
     CDS             38871..39275
                     /locus_tag="VBD85_000258"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7132835.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            39437..39637
                     /locus_tag="VBD85_000259"
     CDS             39437..39637
                     /locus_tag="VBD85_000259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7132836.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            39659..39919
                     /locus_tag="VBD85_000260"
     CDS             39659..39919
                     /locus_tag="VBD85_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7132837.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            40045..40254
                     /locus_tag="VBD85_000261"
     CDS             40045..40254
                     /locus_tag="VBD85_000261"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7132838.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(40427..40588)
                     /locus_tag="VBD85_000262"
     CDS             complement(40427..40588)
                     /locus_tag="VBD85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO7132839.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT        12973 a         6481 c         8045 g        13111 t
ORIGIN      
        1 cattaaaatt ctgtttaaag cgaagtacgc catctgaacc atcaaaattt ccttgaatac
       61 ctaaaaaatt atagaaagtt gattgacgct ttaaagcctc ttgcataaca tattcttgaa
      121 gaactgcagg cgcataaaat ttattaaatt ctgtgtatga gccagagaaa agatagacag
      181 tttctttcgg gctataaata aagaggctac cagctaaaac aacatcttga tttccaaatt
      241 tttgaataaa aggttcgact tctttgcgac gtttatctaa actagctagt tgattgacaa
      301 gctcagtacg ttgacgatgg tatttggcag aatctgtatt attttgatgg cgattatcaa
      361 ggactgtaat ctgttcttcc aatttctttg catttaattg taggttatga tcatattccc
      421 taaagtttag agttgcaatg acaaattccg ccttatctcc gaaactatca taaaaatctt
      481 gataataatc tagagattta tccatatagt cccggcgatc agaagtagaa gaagtaatat
      541 ccttaaagat atgtagttct tcccgcttga ggacacgtat ttttatacca aaagacatgg
      601 cttttttaac caaggggcgt ccttttttag agaaagattt aagaagattt tgaggagtga
      661 taccttctag atttttaaca taatgccaat caggttcacc gccaggataa ccaatatgaa
      721 gcccatcgtg gtgataacct atactagtca ggtcatcaat aagataggtg ttcggtgcac
      781 ctttaggctt accttcacca gtaaactctt ggtaggtatc ataaggttta attaataatt
      841 ctaaagcgcc ctgagatttg gcatagttct gtagttcttt ataaaaatgt ttcagatatt
      901 tagaattgga atgggctgga ccagaattga cttccatatg aagtccacca gtcattggga
      961 tggtataaac aatactgatg atttccattt tcccatcgat ttggtagccc atgtaggtaa
     1021 tgtcataccc acgttttttt agaagctttg ccatctcagg ggtttgcata aacgattgta
     1081 aatcgtaatt tcctgaataa ctttcgaact ctttagctgt taattcttta agcgccataa
     1141 aagtatcctt tcctaactag ctattacgtt ttttgcgtaa ggcatagaca taattagagg
     1201 ctttataaag caatctgttc actggaatgt taaattctcc aataaattcc tcaatgattg
     1261 gttcgaactt agacttaaaa tgatataaac caccagataa gtcattctca atccccccca
     1321 tattttgcca aatatttcca cgttcaaaag ccctttgagc tgtttcaaac caagtgtaaa
     1381 taggagcaga ataagattta aagtaatcgt ccataccagc ataaatattt tctgatgtat
     1441 taccaaattc tagagtgagg gttgcagcca atggaataat gtctcggtca taggcttttt
     1501 gagcttcttt gagaaaatca atttctttat ttaatcgatc aatcgtacct tggtgttgtt
     1561 taactttttt ctcacgatta agttcaagtg attttattct ttcagattgg gctatctgac
     1621 attctttttc tattttttct aatcgttttg caacatctag cgaagccatt gtgatgtatg
     1681 agttattagg atacgtatca aggagttttt ggtagtagtc aattcctcta agatttatcc
     1741 ctttacggtc ttccgttttt ttcatcaact ctgcaaaatc ttcaagtagt tcatggctac
     1801 caaattgtat atctactccc ttatttttgg aagttcgaat agcttgacga gtttttttag
     1861 acattttatc aagtccaaat tgattagcat ataggtttgc ctgaaaacgt ggttggatag
     1921 tatgagctaa atctgttgtc cgaccagtcc aatcagcacc tgcacgctgt aaatgatcaa
     1981 tggcgattaa cccttctttt tcatcgggcg aattttcaaa atctttggca ttgaccattt
     2041 ttaaatagat gagaggatca cacttgataa agagagctct ttgttttttg ccaaaagctt
     2101 taaggtcctt aaggacctta gttacaatat ctagattgga gtaatccatg attggtccac
     2161 gcggaatata tagcatggag aagccaagag gaagtgattt gatgagaata ctagcgctgg
     2221 caatttgggt ttcaccgtca aaaaagccaa gtaattgact accccagttg tcttttactt
     2281 tgggccaatt agaagattga agtaggtttg tttggttgga ttcgctcaca aagcgatcgt
     2341 gttcgacagc ggtaatttct ctatacatga gtttcctttc ttagtgagag tgtctttgtt
     2401 tttttcgaat agtataggct aggttggcca atttatataa taatggattg attgctagat
     2461 taaactcgcc aatatattct tcaaacattg gattgaagtt acttttaaat tttgttaaac
     2521 catcgttaag acttccctcg acaccgccta gattagcaga tacaatatcg tttttgaaag
     2581 catcttcgaa caccctagca ttcaagagat actgtggata aaatttacgg aagctatcat
     2641 ccataccagc atacaacatc tccaggacat tgccaaaacg aatggacaaa attcctgcaa
     2701 tgacaggctc atctggatat ttcttgctaa actcctgaaa ttcaaggata tattgtttaa
     2761 cagaagcttc ttgttgattt agccttgtca ggcgactttt ttgatgggat ggagtttctg
     2821 ataaatcttt ttggatttga agaagttgtt ctttaaattg cgctaatctt ttaggaagat
     2881 tgactttagc taaatagaga taggcatctt ctccataaat tgtcattagt tgatggaaat
     2941 agttttcatt acgtaaagct acacctttac gattctcggt taatgctaca accgtggcga
     3001 atttaggtaa atcatcaatg ttggcacgat aaatttgaac accgcgatgc tttgcatctt
     3061 ttatcagacg tttagtgtgt tttggaaagt tttcttctat atttgctttg gtatagatat
     3121 tcgcttgaaa acgaggttgg atggtttcgg aaatggcttt cgttggacct atccattggg
     3181 cacctgctga cttaagatta tcaattgcct gtctactttc atctatctct tcggcgacat
     3241 tctcttcttt aaggtgatat tgacgtagaa gaagtgctgg atcgaatttt gcaaaaacag
     3301 cacgtttctt tcgtccaaag ttttttagtg tttttaacac aaagttaact aattctttat
     3361 tgctgtagtc cataataggt ccacgtggaa tataaagcat tgtaaaacca agtggtaatg
     3421 atttaattaa gatgctagct acagcaacta attggtcatc tttataaaag cctaaacgtt
     3481 cattttgcca atttgatttt acttgtgccc actttgaaga gtggaggaga ttggtttgag
     3541 acgatgcaat agcaaaggca tcatattctt tttcggatat tccaacggta acatgataca
     3601 tcagtttatt acccattcac gtagtgcgta ggctacgcca ctttcctcgt ttgatttggt
     3661 gatatatttt gctatttttt ttagttcagg attgccgttt tgcataacaa caggatttcc
     3721 aacaacttca agcatagcac ggtcattttc ttcatcacca atagccattg tttgatctac
     3781 agttaatccc attttctctg caaggtggag aagtgcagtg cctttattaa cgtttttggg
     3841 taaaatttct aaataaaaag gagttgattt tacaatagtg tagttatcga gaactttttt
     3901 tggaatttta gcaattgctg catctaagat gtcaggttga tcaatcatca ttagtttaat
     3961 gatttctttg tctcccattt cttcaggagt acgatagaag agcggcatat taactaaagt
     4021 tacttcgtgt attgtatatt taccaatatc acgatttgct gtgtaaattc cttcttttgt
     4081 aatggcgtgc atgtggacgc ctaatttacg cgctaacagt tcgaaatcca gatagtcttc
     4141 ataggttaag gtttctttga tgatgtcatc tcctgtggct gtgtcttgaa ctagaccgcc
     4201 gttaaaggtg ataacataat ccccctcttc attgagtttc aattcttcta aaagatcttg
     4261 gactccgatg attggacgtc ctgtagcaat aacaatttta acgccttttg attttgcttc
     4321 ttgaacagct ttagccactt caggtgtaat ttcacgctta ctatttaata gggtgccgtc
     4381 aatatctacg gcaacaagtt taattgaagt cattttatct cccactcttt attcaatatc
     4441 ctaaaatact caccttatat cttactaaaa aataaagggt ttgtcatgaa tggctattat
     4501 ctttcgtgag tgtcaaatgc tcatttgtga tataagattg aaattcttct tttgtacttg
     4561 aaaataggct atctaatgtc aacatttctt ttggaaaata aaaacgttga tcaccataat
     4621 tagatccagt taatgctttt acaatcggtg ataagcttga taattcagca agttgtccat
     4681 tcttttggat aatttgaatt tctgttcgtg gatttgaatg ttcaggccga taaacatcgt
     4741 aaggtaagtc aaaattagca tggacaccag tgtaataatc gggatcaaaa ccaacctgtg
     4801 agactagttc tttcatttta actagatttt ctttatcaga ctcttcaaag gtaattgatt
     4861 taaagacttt tcgattgata aaacgattag ctaaatctgc caaaatgtta tcatcagctt
     4921 gcatccaaga ttggaagtaa gtattcatta ccccatcatc tagggctaaa taatcttgta
     4981 aatcatattg atccgtgaag aaaggaataa gatttggaga agtttgttca aaaaaatcgc
     5041 gatgggtatc aaataagaag cgagcacgtt ttagtaaatt ttgtaagagt aattccatag
     5101 cacgactggc tggatggaaa taaacttgca tatacatttg gaagcgacta acaatataat
     5161 cttccacagc gtgcatcccg ttacgagcaa aagcaattcc agaatcggtt ggtctaataa
     5221 cgcgtaaaat tcgtgttaaa tcaaattgtc catagctagc agcagtgtag taagaatctc
     5281 tgagaagata gtccattcga tcacaatcaa tttgactaga aattaattga acaacttgtt
     5341 tattggggta gctatgatta ataacactag ctactttatc aggaaaatca ggagccacct
     5401 ttcgtaaaat agcattaatc tctgtggtag gattggtgat gatttcctga gtgtaagctt
     5461 catgatcagt attgaagaga cgctcaaatg tatgagaata tgcgccatgt ccgatatcat
     5521 gtagtagggc ggcagccatc gttagcaggg actcattttt attccaaaga tcagaataat
     5581 gttcatcaaa gatttctgta actttacgag ccagttcata aactccgaga caatgtgaaa
     5641 aacgactatg ctctgcacca tggaaggtaa aagaagtcgt tgaggtttgt ttaatgcgtc
     5701 gtaaacgttg aaattcttta gtgttaatca agtcatatat gacttgatta tttacatgga
     5761 tataagtgtg aacggggtca cgaaatactt tttcattcat atatatatta tagcaaaaat
     5821 tgaaacttga gtcagaaatt ggtagaatag aaggataaaa gaagtgagag aagtcatgaa
     5881 actagaaatt aacaataaca ttcaaattga caacgaaacc gaaatgattc atgagattca
     5941 cgattgtcaa tttattgaaa aaggaagtta cgtttatttg aattatatta atgctgaggg
     6001 cgagagggta gttattaagg caaatcatga ggaattgctt atgacgaggt tttcaaatcc
     6061 taagtctgta atgcgttttc atcgtgaaac gcctgctttg gttaatatcc ccactccact
     6121 tggtgttcaa catttgatta cagagacaag tcactatcag tttgatttgt cacagcaaag
     6181 gttgcatatc aattatgtcc ttaagcaaac ggagacagga gactgttttg caaattatga
     6241 attaaggatt caatggtatt aagataacta ccattaacaa aagagtagtc gttatgaatt
     6301 tcgtttactg aagacgctgg gatctcttta tgagagccat ttatttgata aactgcagtg
     6361 tggtttattt cgactagatg aacttcgttt tttcccttga gattattagt gatagcaagg
     6421 ataattaatt gaaaaatgga tttttttctg ttacaatgat taacatatca agggagtagc
     6481 agacggttta ccgtggcaat gtcgtcatta cgaaagaaat ttccggcgtt gtcttaaaca
     6541 gcgagacttg ttttcataaa aacaggtctc tttttgtttg tggagagacc aaaggaggaa
     6601 aggttttgtt aaaagaacag aaaaaatcct tgttttacac acaaggtcaa gaagaggttc
     6661 taacgtcttt agaatcttct cgtgaaggat tatcaacgac agaagcgaaa aatcgcttag
     6721 agatgtatgg tcgcaatgag cttgaagaag ggaaaaaacg tagtctaata gctaagtttt
     6781 ttgaccaatt caaagattta atgattatta tcttactggt tgctgcagct ctctctgtta
     6841 ttacggaagg tatgcatggc ttaacggatg cattgattat cttggcggtt gttatcttaa
     6901 atgcagcttt tggtgtttat caagaggggc aagccgaagc agctattgaa gccctcaaag
     6961 atatgtcaag cccaattgcg cgtgttcgtc gtgatggtca tactattgag gtggattcta
     7021 aagaactagt tcctggtgac ctcgttatgt tagaagcagg agatgttgtt cccgcagatt
     7081 tacgactttt agaagccgcg tctctaaaaa ttgaagaagc agctctaact ggtgagtcag
     7141 tacctgttga gaaagacata tctcaagtag tagcagagga tgcaggtatt ggtgaccgtg
     7201 tgaatatggc gtatcaaaat tcaaatgtca cgtatggtcg aggatatggt gttgttacta
     7261 acacagggat gtatactgaa gttggtaaaa ttgcggacat gttggcaaat gctgatgaat
     7321 ctgaaacacc attgaaacaa agtttagttc aactttctaa attattgact tacttaatcg
     7381 tgattattgc ggttattact ttcttagtgg ggatatttgt ccgtaaagaa ggttggattg
     7441 aaggattgat gacttcagtt gctttagcag ttgcagctat tccagaaggg cttcctgcaa
     7501 ttgttacgat tgtcttatca atgggaacaa aaactcttgc taaacgcaat tctatcgttc
     7561 gtaaactccc tgcagttgaa actttaggtt ctacagaaat cattgcatca gataagactg
     7621 gaactctgac aatgaatcaa atgactgtcg aaaaagtgta tactaatggt gttctccaat
     7681 catcttcgga agaaatttca gttgataata acactttacg tattatgaat ttctccaacg
     7741 atactaagat tgatccttca ggaaaattaa ttggtgaccc aacagagact gcactggtac
     7801 aatttggttt agataagaat tttgatgtcc gtgaagtttt gaaaaatgaa ccacgtgtag
     7861 ctgagttacc atttgattca gatcgtaagt taatgtctac gattcataaa gaatcagatg
     7921 gtcgctactt tatcgcagtc aagggtgctc ctgatcaatt attaaaacga gttactaaaa
     7981 ttgaagataa tggcttggtt cgtgatatca cggctgaaga taaagaggct atcctaaata
     8041 ccaataaaga actcgcaaaa caagctcttc gtgtcttgat gatggcttat aaatatgaaa
     8101 cacaaattcc aactttagag actgatattg ttgagtcaga tttagtattc tcaggtttag
     8161 tgggaatgat tgaccctgag cgtccagaag cggcagaagc tgttcgagtt gctaaagaag
     8221 caggaattcg tccgattatg attaccgggg accatcaaga tactgcagaa gctattgcaa
     8281 agcgtctagg tattattgat gcaaatgaca cagaagatca tgtctttacg ggtgctgaac
     8341 ttaatgaatt aagtgatgaa gagtttcaaa aagtatttaa acaatattct gtctatgcac
     8401 gtgtttctcc tgaacataaa gtgcgtatcg tgaaagcttg gcaaaacgat ggcaaagtcg
     8461 ttgccatgac tggtgatggt gttaatgatg ctccatcgct aaaaacagca gatattggta
     8521 ttggtatggg aataactggt acggaagttt ccaaaggtgc ttctgatatg gtccttgctg
     8581 atgataattt tgcaactatt attgtggctg ttgaggaagg acgtaaagtc ttctcaaata
     8641 tccaaaaatc tattcagtat cttctttcag ccaacatggc agaggtattt acaattttct
     8701 ttgcaacttt attaggatgg gatgtacttg caccggttca ccttctttgg attaacttgg
     8761 taacagatac tcttccagcc atcgctcttg gagttgagcc agctgaacca ggagttatga
     8821 cacataaacc tcgtggtcgc cagtcaaact tctttgatgg tggtgttatg ggagctatta
     8881 tctatcaagg tattttacaa actatcttag tacttggtgt ctatggttgg gcattgatgt
     8941 atccagaaca tgctggttat cgcatgattc acgcagatgc ccttacgatg gcctttgcaa
     9001 cgcttggttt aatccaattg gtacatgcat ttaacgttaa gtcagtttac caatcaatct
     9061 ttacagttgg agcttttaaa aatagaacct ttaactggtc tattccagta gctttcattc
     9121 ttttgatggt gactattgtt gttcctggat ttaataaact tttccatgta actcatttaa
     9181 gttcaacaca atggttaact gtagttatcg gaagtttatt gatggtagtg ttaacggaaa
     9241 ttgttaaatt tatccaacgt aaattaggac aagacgaaaa agctatttag tttagctgat
     9301 aaaaaaactc acttagaata tctaagtgag ttttttagca atcaaatatt gtcatagagt
     9361 tgcgtaattc gtcttcaaaa tatttttttc gatagttttc aaaactatcc aaatgcttaa
     9421 cggcattata ccttttgcta agattccagg ttaaattagg gttatgattg ggattctgat
     9481 tgacaaagtc aatagttaaa ttccattctc taatatatcc aagtggtcta gaatgataag
     9541 tagtctctcc aattttaacg tcgccaaatg agcggtgtgc atgaccaaaa acaacatctt
     9601 tgatatgata tttttggaat aaatcatgat aagcctgact ccccaaaaaa gcattaaaag
     9661 gagagaatct aggatgagtc atcgtgaatt gtttatgggg aacaaagtgc atagcaataa
     9721 tgatatttga agaatcgact tttgctaata tttcgtctaa gcgttttaaa attgatgctt
     9781 ggatagttac atcattattt ggacgtttta aacgtctatc aaaccaaaaa gtctttttta
     9841 atttttcaac atccttgatg tctctattat ttgaaaagct gtaatcatac cagccatgaa
     9901 aagctagtag catttttttg tcaaatcgat aggtttgaaa gtcaagacgt tggatttctg
     9961 tttctgttaa atcaagcata tcatggtttc ctaaattata gctaagttta atatgctttt
    10021 ttaagttatt gataaaggga agtgtatctt tagtaaaatg attagatata tctccagcga
    10081 tatggaggtg atcaattttt tctttcttaa gcaaattaat taacgttttc gtctcaaagg
    10141 tagaaaaatg attgaggtca atatgtaaat cactcatgat agcaagtttg gtcataaggg
    10201 tattgtacca tattgtagtt caaagagtta aacagtaaat ttttgaaata gttttagagt
    10261 cattctataa tagatatgtc attgtagaat gcgctttcat aagtgtgttt agaagtttta
    10321 tatttaaggt gccttaagga ggtctcatgt ctaattttta taagctcttg aaagaaaaat
    10381 ttcctcgaaa agaagatatc gtaacagaaa tgatcaattt agaagctatt tgtcaattac
    10441 ccaaagggac ggagtatttt attagtgatt tacacgggga atacgatgct gttgattatc
    10501 tcttacggac aggtgcaggg tctattagag ctaaattatt agactgtttt gattggcaga
    10561 aaatagttgc tgttgattta gatgattttt gtatactgct ttattaccca aaggaaaaat
    10621 tagccttcga taaaatgaac ttgtcagcaa gtgcttataa aacgaagtta tgggaaatga
    10681 ttccgttaca aatacaagtt ttaaaatatt tttctagtaa atatacaaaa tctaaagtac
    10741 gtaagcaatt gtcaggtaag tttgcttaca ttatcgaaga acttttagct gagattgacc
    10801 gtaacccaga gaagaaaagc tattttgata ctattattga aaaattattt gaattagacc
    10861 aagtagaaga tttaattata gtattgtccc aaacaattca agttttgatt attgaccatt
    10921 tacatgttgt aggggatatt tacgatcgtg gtaggtatcc tgatcgtatc ttgaataggt
    10981 taatggcttt tccaaatttg gatatccaat gggggaacca tgatgtcact tggatgggag
    11041 cagcatctgg ttcttacctt tgtatggtaa atgttatccg tattgctgca cgttataata
    11101 atattacttt aattgaagac cgatatggta tcaatttgcg tcgcttggtt gattactcac
    11161 gccgttacta tgaacctcta ccttctttcg ttcctatatt agatggagag gaaatgactc
    11221 atccagatga actagacttg ttaaatatga tacaacaagc aacagcaatt ttacagttta
    11281 aattggaagc ccaattgatt gacaggcgtc cagaatttca aatgcataat cggcaactta
    11341 ttaaccaggt aaattataaa gatttaagta tatctattaa ggaagttgtg catcaactta
    11401 aagattttaa tagtagatgc attgattcga aaaatcccag tcgattaaca agtgaggaag
    11461 aagagctttt acaacaattg atgatagcct tccagacttc tgaaagttta aagaaacata
    11521 ttgatttcct atttgaaaaa ggaagtatgt atttaacata taatgataat cttttattcc
    11581 atggttgcat accaatgcat agtaatggtg attttaaaag ctttaaaatt gcaggaaaaa
    11641 cttatggtgg tagggattta ctggatttat ttgaaagtca aatacgtcta gcatatgcta
    11701 gaccagagaa acatgatgat ttggcgacag atatcatttg gtatctttgg tgtggtgaaa
    11761 attcttctct ttttggtaag aatgccatga ctacttttga gcgctattat gtttccgaca
    11821 aagtaactca tcaagaaaga aaaaatccct actttaaact gagagataaa gatgatattt
    11881 gcactgctct tttacaagag tttgatttgc ctaagtttgg gcatattgtc aatggtcata
    11941 cgccagtcaa agaaaaaaat ggtgagcaac cgataaaagc aaatggtaag atgttagtaa
    12001 ttgatggtgg ctttgccaaa ggttatcaga aaaatacagg tttagctggc tacactttga
    12061 tttataatag ctatgggata caattaattt cacaccttcc ttttacatcc attgaggaag
    12121 ttttaagtgg aaccaactat atcattgata cgaagcgatt agtagaagaa gccaaggata
    12181 ggattcttgt aaaagataca actattggac aaaagttaac caaagagatt aaagacttag
    12241 atcaccttta tcgtcatttt caggaatatg atgactagtg tttaagtaat ttactaattg
    12301 atatgtagga tttaattttt cagtttctta ccttgtctct tttattaaat tgacttaaaa
    12361 taatattatg gacataaagg cagaaattca aaaattagca aaagaaattg gtatctctaa
    12421 aattggtttt acgacggcag ataactttga ttatttggaa aagtcattgc gtgcttctgt
    12481 ggaagagggg cgcaattcag gatttgaaca taaagtcata gaggatcgta tctaccctga
    12541 aaggctttta gaatcggcta aaacgattat ttcaattggc gttgcttatc ctcacaaact
    12601 tcctcagcaa cctcaaaaaa catcttataa acgaggaaaa attacaccta attcgtgggg
    12661 gcttgattat cattatgtgg tcggcgaaaa gttagacaga ctatcaaaag gcattgaaga
    12721 gctctgtcga gattttcctt tgcaacaaaa agcaatggtc gatactggag ctttagttga
    12781 tactgcagtt gcccaaagag caggtattgg ttttatagga aaaaatggtc tagttatttc
    12841 taaagaatat ggctcttata tgtttttggg agaattaatt accaatttag agatcgagcc
    12901 agacaagcct gtcgattatg attgtggtga ctgtcgtcgt tgtctagatg cctgtccaac
    12961 ttcttgttta attggtgatg gctcaatgaa tgccaagcgt tgtctatcat ttcaaaccca
    13021 agataaggga atgatggaca tagagtttcg aaaaaagatt aaaacagtta tctatggttg
    13081 tgatatttgc caaatttgct gtccttataa caaaggtata aataatccat tggcgacaga
    13141 gattgatcct gagttggctc aaccagaatt aataccattt ttgagtttat caaatggaca
    13201 atttaaagaa aaatttggta tgattgctgg aagttggcga ggtaaaaata ttttacagcg
    13261 taatgccatt attgcgctag caaatgctca tgataaaacc gcagtggtca aattaataga
    13321 gattattgat aaaaataata accctattca tactgcaaca gctatatggg cattgggaga
    13381 aattgtcaaa aaaccaaacg atgagatact ggagtttatg agtaatttaa ccttaaaaga
    13441 tgaagatagc cgaaaagagt tggaattaat ccgtcataaa tggcaatttt aagcctttta
    13501 tggtattttt aagaacctta tgataaaata aaacagatta acaaagattg gagaaatctc
    13561 atggaagtgg ctgaaattcg ccaaaaaata gtagaaaata aagagaagtt gactagcttc
    13621 aggaggtctc tttgacttag atcgtttgga agaggagatt gctcttcttg aaaatcaaat
    13681 gactgagcca gacttttgga atgacaatat tgcagcgcaa aagacatctc aagaactgaa
    13741 tgagttaaaa ggtaagtatg atactttcca taatatgcaa gaattatctg acgaaacaga
    13801 actcttactt gagatgcttg acgaagatga ttctttgaaa gaagaattag aagaaaatct
    13861 aatgcagcta gataagataa tgggtgctta tgaaatgacg cttctcttat ctgagcctta
    13921 tgaccacaat aatgctatct tagaaatcca tcctggttca ggaggaactg aagcgcaaga
    13981 ttggggagat ttactcttac gaatgtatac ccgttttggc aatgctaatg gtttcaaagt
    14041 agaagtatta gattatcaag caggggatga agctggtatt aaatcggtta ccctctcttt
    14101 tgaaggcccc aatgcttatg gtttgttgaa atcagaaatg ggagttcatc gcctcgttcg
    14161 tatatcacct tttgattcgg ctaaacgtcg tcatacctca tttgcttcag ttgaagtgat
    14221 gccagagtta gatgatacga ttgaagtaga agtacgtgat gatgacatta aaatggatac
    14281 tttccgttcc ggaggagctg gtggtcagaa cgtcaataaa gtatcaacag gtgttcgttt
    14341 gacgcatatt ccaactggaa ttgtggtatc gtctaccgtt gaccgtactc aatatgggaa
    14401 ccgcgatcgc gctatgaaga tgttgcaggc taagctatat cagttagaac aagaaaagaa
    14461 agcacaagaa gtagatgctc ttaagggtga taaaaaagaa atcacttggg gaagtcagat
    14521 tcgttcttat gttttcaccc cttatacgat ggtgaaggat catcgtacta actttgagct
    14581 tgctcaggta gataaggtta tggatggaga aatcaatggc tttattgatg cctaccttaa
    14641 gtggcgcatt gaagactaaa aataatgaag gagaaacatg gcactaatag aaatgtctgg
    14701 tgttactaaa aagtatcgcc gttcaacaac tgccttgaga aacttaaatt tatcaattca
    14761 gcaaggtgag tttgtgtatt tagttggtcc gtcaggtgct ggtaaatcta gcttgattcg
    14821 cctcctatac cgtgaagaaa aattaagttc aggtagatta aaggtagggg aattcaatct
    14881 caataaactc aaacgtcgtc aaattcctat tttacgtcgt agtattggag ttgtcttcca
    14941 agattataaa ttactaccaa caaaaacagt ttatgagaat gttgcgtttg cgatgcaagt
    15001 tattggagct aagcgccgtc atattaaaaa acgcgtgcca gaagttctag aattagtagg
    15061 tttaaaacac aagatgcgtt cttttccaac acagttatct ggtggagaac aacaacgtgt
    15121 ggcaattgca cgtgcgattg ttaataatcc aaaattatta attgcagatg aacctactgg
    15181 taatttggat ccagaaattg cttgggaaat catgcatcta ttggaacgta ttaaccttca
    15241 aggaacgact gttttgatgg caacacacaa tagtcaaatt gttaacacac ttcgtcatcg
    15301 tgtcattgag attgaagcag gtagcgttat ccgagatgaa gagaagggag aatatggtta
    15361 tcatgattaa tttctttaga catttctggg aatcgcttaa aaacttgaaa cgtaactttt
    15421 ggatgacttt tgcttctgta acatcagtta ccataacctt acttcttgta ggcttgtttt
    15481 catcagtttt actgaatgtt gaaaagttaa caactgatgt ttcaggtaat tttactatca
    15541 gtgccttttt aaatgttgac tcaacggatg ctcaaaaaca agtcaaagat aaggacggaa
    15601 agctaaaaga taatcctgat tatcataaag tttacgacaa aattaaacgt atttctggtg
    15661 tagagaaagt cacttattca agtaaagctg aacaattaaa agaagttcaa aaagagtatg
    15721 gttcagatgt tattgatgat acctataaag atgctttgtt agatgtttac gtagttggga
    15781 caagttctgc aaaagtttct aagagtgtca gtgaagcaat tggacgcatt gaaggtgttg
    15841 attatactaa agaacctatt gattcaacaa aactatctaa tctcacagat aacatccgta
    15901 tatggggatt tggtggtgtt gccttattaa ttgtccttgc tatctttttg atttcaaata
    15961 ctattcgtat gtcgattatg tcacgccgta cagatattga aatcatgaga ttagttggcg
    16021 ctaaaaattc ttatattcga ggaccgttct tttttgaagg tgcttgggta gggattttag
    16081 gggctatcgt tccatcgctg attttctatt ttggttatca atttgtgttt aacaagttta
    16141 atccaaaatt tgagacaagc catgtttcat tgtatcctat ggatattatg gttccagcaa
    16201 ttattggtgg tatggtgatt attggtatta ttattggctc acttgggtct gtcctatcaa
    16261 tgagacgtta ccttaagatt taataaaaag agatgaaatc tatttcatct ctttttatta
    16321 attgtagata tcatgttagt ggataaaatt cgaaataaac gtagccaatt gtttttcaga
    16381 tgatttgtga gaaagggtag ctttaccatc ccctttttga tgtccgtagt taccaaatcg
    16441 gctatggttt cctccgacaa tagttcgaaa agtacttgaa tttgggaggc gttttttagc
    16501 ttcctcatat ttttcccagt ttaaaatatg atcattactt gctgtaatgg agagtactct
    16561 taacttttta tgagataggt cggatttacg tgaagggtag gaagctaata atatgagccc
    16621 tctaacaggg gctactttgg cattttggct agcaacaact cctcctaaag aatgtccagc
    16681 aaggtaaaca ttagttaagt ggttctgatt aataattgtt ttagctttat gaggcgataa
    16741 aactggcaaa tttaaaggtg tcttcaaaat ataggtatta tcccctttgt cagctaaatc
    16801 tctagcaagt tgagaataag cttcttcttc cacaagagcc ccttgataga aaataatatt
    16861 tgcttttacc ttacccttgc ttctaaaaag aaggtagttc ttatggtttg tagcggtttt
    16921 agcaattctc ctagctaatg gggaggcttg gtaagtattg aagtaaatgg tcatcccgac
    16981 ggccattaaa atagcaagga tacttgttgt taaccaaaga aaaatagagt gacgttttat
    17041 gttcattgga agaaaggatt aaagtttttt tcgtggccaa tagttgtatt catgccatgg
    17101 cctgggtgaa cactataatg actaggtaag gtgaatagtt cttgacgaat accagtaatg
    17161 aggtcctcaa agttactggt aggtaagtca gtacgaccga tagtttcacg aaatagagca
    17221 tcgccagtaa cgactaattc atctgagtgg aaaacaaacg agactcctcc ccaagaatga
    17281 ccaggtgttg gtaaaactgt gaattcaaaa ccatttagtt gatagggttg ctttaattta
    17341 aaaaagttct ctgccggtct tgctataaca ttaatgatat catcatgacg gcctaaccca
    17401 gataaattat catctgggga agatagccaa gcggcttctt tttctgaaac ataaacaggt
    17461 ggattatcaa aagtatctct gaccaaatct aaactaaaaa tatgatcata gtgagtatga
    17521 gttaagagga tagccactaa aggtttttca aaggatttaa ttttagcaat aatttcttgg
    17581 ccattgctcc ctggatcaat gaggataaca gcttgatcat taacgaggta ataagtattt
    17641 tcaccagcaa tgtggtttat tgttttatag attttcataa tgatagtgta ccaaaaaatc
    17701 aagactttgt taaaaaaaga gtggcggaaa ataaatggca actagtttca gaaaatatgg
    17761 gatctcacag caatttcttg acaccgtttt cataaaggtg tatcataaaa gtaatcaata
    17821 agccaagcct aaagctgaac ttagtttaag aacactgact ttttgagagc aacctaattg
    17881 gttgactaaa gaatatgagg tggaagatat gtcaaaagtt gctattgtta ctggtgctgg
    17941 tcaaggaatt ggatttgcta ttgcgaaacg tcttcatgca gacggcttta aaataggcgt
    18001 tttagactat aatgaagaga ctgctcaggc tgcggttgat aaactatcac ctgaggatgc
    18061 tgttgcagtt gtagcagatg tttcaaaacg tgatcaagtt tttgatgctt tccaaaaagt
    18121 tgtagatact tttggtgatt tgaatgttgt tgttaacaat gcaggtgttg ccccaacaac
    18181 tcctcttgac actattactg aagagcagtt tgaaaaggct tttgcaatca atgtcggagg
    18241 aactatttgg ggaagccaag cagctcagaa acattttaga gaattaggtc atggcggaaa
    18301 aattataaat gcaacttcac aagcaggatg tgaagggaat cctaatttga ctgtttatgg
    18361 tggtacaaaa tttgcagttc gtggtattac acagactttg gcaaaagatt tagcatcaga
    18421 aggaatcaca gtcaatgctt acgcaccagg tattgttaaa acacctatga tgtttgatat
    18481 tgcccatgaa gttggtaaga atgcaggcaa agatgatgaa tggggtatgg agcagtttgc
    18541 caaagatatc actttgaaac gcttgtctga accagaagat gttgctaatg cagtaggctt
    18601 cttagcggga gatgattcaa attatatcac tggacaaaca atagttgtcg atgggggtat
    18661 ggtattccat taaaaatgat taatgctaac actgtgagtt tcactcacag tgttttttat
    18721 atttcttaaa aaattatctt atcttggtta ttggagtgat ggcatctttg aggtatagtc
    18781 atgttttgtt ttatagatat agcgtgctat aatagactta ctatgaccca gaaaaaactt
    18841 agaaaatatg cagtagttga cttggaggct actggagcag gtcccaatgc ttctattatt
    18901 caggttggga ttgttattat ccaaggaaat aaaattattg atagttatga aacagatgtt
    18961 aatcctcatg aatctttgga tgagcatatt gttcatttga caggtattac ggataagcag
    19021 ttggctaaag ctccggattt tggtcaagtg gcgcatcaca tttatcaatt gattgaagat
    19081 tgtatttttg ttgcccacaa tgtaaagttc gatgcaaatt tattggctga gcaactattt
    19141 ttagaaggct gcgagttacg tacaccaagg atagatacag tagaattatc acaagtattt
    19201 tatccctgtt tagaaaaata ttctttaggg gcattagcag aaagcttaaa tattgaatta
    19261 acagatgcgc acactgctat tgcagatgca agagctacag cacagttatt cattaagtta
    19321 aaagctaaaa ttagtagttt acctaaagaa gtgttggaga ccattttaac atttgctgat
    19381 aatcttttgt tcgaatccta tttattgatt gaagaagctt atcaagaagc tgatttcgtt
    19441 aatcctaaag aatattattt ctggcaagga ttggtcttaa aaaaagaaaa agctgttgga
    19501 aagcctaaga aattatctag tgattttcaa gttaacatgg ctttgttagg tatggatgct
    19561 cgccctaagc aagttgtttt tgctgacttg gttaaagcgc attttaatga tcaaacaaca
    19621 acgtttttag aagctcaacc aggacttggt aaaacatatg gttatctctt gcctttattg
    19681 gatcagtctc aaaaacaaca aataatagta tctgttccaa caaagatttt gcaagatcaa
    19741 ataatggcta aagaaataaa gcacattcaa gaactttttc atattccctg tcacagtatc
    19801 aaaggaccga gaaattactt gaagttagat gcgttttata agagcctcca agtgcaggat
    19861 cgaaatcgat tgataaatcg ttttaaaatg caactgctag tgtggttgac ggaaacgact
    19921 acaggtgatt tggatgaaat taaacaaaaa caacgattag aatcttactt tgaccagcta
    19981 aaacacgatg gagaagtgac tcaatcatca cttttttatg atctggattt ttggaaacgc
    20041 agttatgata aagttgcaca aagtcaactt gttattatca atcatgctta ttttcttgag
    20101 cgtgttcagg atgacaaaga ttttgcaaag gggaaggttt tagtttttga tgaagctcag
    20161 aaacttgttt tgggactgga aaatttttct cgtgggcaac ttgatattag tcaccaattg
    20221 caggttattc aaaaaataat tgatagttct attccattat tacaaaagcg ccttctggaa
    20281 agtatctctt atgagctaag tcatgcagta gaactttttt atcgtcataa ttcttttgaa
    20341 ttttcagaga cctggttgaa acgtctaaaa aatagtataa acgctttaga agtagtagga
    20401 ttagatgagt tacaaacttt tttcacagct acttatacta actattggtt tgaaactgac
    20461 aaagtcaatg agaagcgttt aaccatttta cgtggggcga gagaagattt tcttaaattt
    20521 tcaaagttcc tcccccccac aaaaaagacc tatatgatat cagcgacttt acaaatcagt
    20581 cccaaagtct acttgtcaga tcttttagga ggattttcaa gtataagtac agaaaagata
    20641 gcgcatgaga aaaatgccaa tcagaaagta tggattgata cctctatgcc caatatactt
    20701 gatttatcac cggaacagta tgcttacgaa atagccaagc gtttacaaga tataatgacg
    20761 ttaaagcagc ctaccttagt cttattaact tctaaacaga caatgtttat ggtatctgat
    20821 tatttggata agtgggagat aaaacatctt actcaagata aaaatggctt agcttataac
    20881 gttaaaaaac gttttgatcg tggagaaagc aatcttcttt taggtacagg atctttttgg
    20941 gaaggtgttg actttgtaca tcgtgatcgt cttattgaag ttatcacgcg attgcctttt
    21001 gatactccca aagactattt tatccagaaa ctatcgcaga gtttaacaaa agaggggaaa
    21061 aacttctttt atgactattc tttaccaatg actgtcttaa aattaaaaca ggccttggga
    21121 cgtactacaa ggagagaaga acaaaagtca gcggtaatta ttttggatag cagattggtt
    21181 attaaatctt atggacaaac catcatgcat tctcttggta gagattttga aatttctaag
    21241 gaaaaaatta acaaggtctt gactgaaatg gctaaatttt tgatataatg gtaaagatca
    21301 ttgtctaaaa ataagatttt gacagtgcga ctcaagatta aataatagta gtacgttgat
    21361 aaaaggagga gtaatgacct atttgtcaga acgtgtactc aatatggaag agagtgttac
    21421 tttagctgcg ggagctaaag caagagaact cagagtacaa ggaagagata ttcttagttt
    21481 aactttaggg gagcctgact ttgctactcc taaaaacatt cagcaagcgg ctattgaggc
    21541 tataacagat ggacgtgcaa gtttttacac accatcaagt ggtttgccgg agttgaagtc
    21601 agcaattaat gcgtattttg aacgttttta tggctactcc cttaaaccta atcaggtggt
    21661 tgtgggaact ggtgctaagt ttatccttta tactttcttt atgacagtac tgaatcccgg
    21721 tgatgaagta attattccga ctccttattg ggtatcttat gctgatcaga ttaagatggc
    21781 tgaaggtaag ccagtttttg tcacagcaaa agaagttaat cacttcaaag taactgtgga
    21841 acagttagaa gctgttagga ctgataagac aaaagtcata cttcttaata gtccatcaaa
    21901 tcctacaggt atgatttata aagctgagga gttggaggct attggaaatt gggcagtcga
    21961 acatgatatc ctaattttag cagatgatat ttacggtcga ttggtttaca atggcaatat
    22021 ttttacccct atttcaagcc tttcagagtc tattcgaaac caaacgatag tcataaatgg
    22081 agtttctaag acttatgcga tgacaggttg gcgtgttggt tttgcagtgg gtaatcatga
    22141 tattatagcg gcaatgtcta aagtagtcag tcaaacaact tctaacctta cggctgtctc
    22201 acagtatgct actattgagg ctttaaatgg ttctcaagag tcatttgaaa aaatgaggct
    22261 agcatttgaa gaacgcctta atatcattta tccattgttg tgtcaggtcc ctgggtttga
    22321 ggttgttaaa ccacaaggtg ctttttatct tttcccaaat gttacaaaag caatggaaat
    22381 gaaaggatat acggatgtta cagcattcac ggatgccatt cttgaagaag ttggacttgc
    22441 tttagtaacg ggtgctggtt ttggagcgcc ggaaaatgtt cgtctgagtt atgctacaga
    22501 tcttgaaacc cttaaagaag ctgttcgtag actgcatgtc tttatgggaa gtaatgaaat
    22561 taattaaata aaaatagaga gaaacttatg tcaaaaaaac ttatttcaat tgttgatgtt
    22621 aaagattatg ttggtcaaga agttactatt ggtgcttggg ttgctaacaa atcaggtaaa
    22681 ggtaagattg cgtttgtaca gttgcgcgat ggttctgcct ttttccaagg tgtcgctttt
    22741 aaaccaaact tcattgaaaa atatggtgaa gaatcaggtc ttgaaaaatt tgacgttatt
    22801 aaacgtctca accaagaaac atctgtttat gtaacaggga ttgttaaaga ggatgaacgt
    22861 tcaaaatttg gctatgagct tgatattact gaccttgaag tcattggaga gtcacatgaa
    22921 tatcctataa ctcctaaaga gcatgggaca gacttcttga tggacaaccg tcatctttgg
    22981 ttgcgttctc gtaaacaaat ggctgttatg caaattcgta acgctattat ttattcaact
    23041 tatgaattct ttgaccaaaa tggttttatc aaatttgata gcccaatttt atcagaaaat
    23101 gcagcagaag attcaacaga gttatttgaa acagactatt ttgggaaacc agcatttctt
    23161 agccaatctg gtcaattata ccttgaagct ggagcaatgg ctctaggccg agttttcgac
    23221 tttggtccag tctttcgtgc tgaaaaatct aaaacacgtc gccatttaac agaattttgg
    23281 atgatggatg ctgagtattc tttcttgtca catgaggaat cacttgacct ccaagaagct
    23341 tatgttaaag cattgatcca aggcgttcta gatcgtgcgc ctcaagcact tgatatttta
    23401 gaacgtgatg ttgaggctct caaacgttat atcgcagagc cattcaagcg tgtttcttat
    23461 gatgatgcta ttacacttct tcaagagcat gaagcagatg aagatacaga ttatgaacat
    23521 ttagagcatg gtgatgactt tggttcacca catgaaactt ggatttctaa ttactttgga
    23581 gtgccgacat ttgtagtgaa ttacccagca agctttaaag cattttatat gaaaccagtt
    23641 cctggaaatc cagaacgcgt tctttgtgct gatcttctag ctccagaagg ttatggagag
    23701 atcattggcg gctcaatgcg tgaagacgac tacgatgctc ttgtggctaa aatggatgaa
    23761 ctaggtatgg ataaatcaga atacgacttt taccttgacc ttcgtaaata cggctctgtt
    23821 cctcatggcg gttttggtat aggtattgaa cgtatggtaa cttttgttgc tggtaccaaa
    23881 catattcgcg aagctattcc attcccacgt atgttacatc gtatcaaacc ataataaaaa
    23941 agcgctagct agtgcgcttt tttattatgg tctttagaat cgttggttaa ttaaattggg
    24001 actatgtttt tttaacaatg tgattgtcga aaagacaagg ataagactga ttagtcgatc
    24061 catataatct gttccaactt gaacaagtat caaactactt gtcatgtcta atccaattcc
    24121 atgtaagaat tgtgctataa tacttgagcc actagaggta attcctttaa aaaggataac
    24181 tgttataact gaagcaataa tagttcctgg taaagaaata atcagtgatt tccacaatag
    24241 gctagagggt ttacaattcc ttttaatcaa gatacccgta atgatagcaa ccacgatggc
    24301 gactggtgaa taataaagtg aaaaaaatat cagtagtcat ccaattgatg agtgcactga
    24361 gaaaagcagt gctggcagcg taccatggtc ccaggataac tgctattaac aatgtaccta
    24421 tagtatctag atagataggt aggcgtaaaa atagagctaa attagcccca acaaggttga
    24481 tggcgatagc gattgagata aatgaaatta aacgagcagg tgattttgtc atttgttaag
    24541 tcctaacatt tctaagtctt caatgatgac tgaattttgt gcatgtaata ggacggtcat
    24601 aaaatcattc caaaataaat tagtattgac tgaggttaaa atcttactgt tagcatcttt
    24661 tttataaaaa tgatactgat ctacaattgt ttgtcccatg gcgattccgt gacaagcaac
    24721 atcggtatag ctatcaaaac cagtagcgat attttcatta acaaagtaag caatagctaa
    24781 aggatcattt atcacacatc caataatatg ctcatattgc cagtgaaaat cgaagtagaa
    24841 ctttgtgatt ttttggataa aacttgatac gtctggatta atacgctcca tataagataa
    24901 atgattgggg gttaatacaa tatgacgtgt gatgtctagt ccaaccatct ctattttttt
    24961 gtcaaggttt tcaaatacat attgtgcagc atgaggatca caccaatagt tgtattcagc
    25021 aactggagaa caattgccat gtgatttaaa acttcctccc atgctaataa atcgcttgca
    25081 atgtttgcct aatttgggat tagtttgtaa ggctcgagcg atattggtca gaggtccgag
    25141 agctataata gaagtatcat ttttgtgttc aaaatagttt gctaaaaaac aatctgctga
    25201 ttcttcttga aagataattg gttgcgccaa tgtaaagtta ttctctccaa ggccatccat
    25261 tccatgagta tcctgagcag atacaaattc acgttgtaaa ggcaagttat caccaacata
    25321 aactggaatg tcgtgacggt ttagtaattc aagcgttaca aaggtattct ttagtcctag
    25381 ctcaacaggc gaattgcctg ctgttatagt aatagcaaca acttctaact taggatgctg
    25441 aatagcatac atcagtgcta aggtatcatc aataccggga tcgcaatcga taataatttt
    25501 ttctttattc atttgtctcc tttcttttaa tattagtgaa tgttctatgc ttttatgaaa
    25561 ctattaacaa tagtcatggc aataaaaaag atcttggcag ttaccaagat cacacgaaaa
    25621 aatgaattta gttttctaaa ttcaatcata ttttcagtca cttagttttt tattctggga
    25681 agttttcgaa ccagtccgag aaatactcga ttatgcacgt gttttattat aatatgaaag
    25741 cactaacttg taaagattca aactgaccat ttaacagtgt ctagattgat tgttttagca
    25801 tttgaaatta attttgaaat cggacatctg ctgtgtgctt tgttaacgat tagcttacta
    25861 tcagataatg acagtttttc aatactagca acagcgttaa cttggaaata aaatccctta
    25921 cctacttgct tttccttcat gagctggcaa gtaacatcaa cgcgagattt taggtctttg
    25981 aatccttttt gttctaaaat agcttttata gttgcattta aacatgttga ccatgctaaa
    26041 gcaattaatt cctcaggatt aaatcctggt aaatgattta aagggtgatt ggtctccaca
    26101 atttttccat tggagagata aacttttcca tcaacgcctt gagtgtttga tgaggttact
    26161 tcataaagca taataaactc ctttgggtct agtttaccaa atttgggagc attccgacaa
    26221 gattttacta tttagaatga gtttcttttc ctctgaagta aaatcctaat tgctagccat
    26281 ttgtggtata atggcataaa acataagaga aagaggtaat cgtaatgtca gacgaacaaa
    26341 tcaaacttgt gattgtaaca ggtatgagtg gggcaggaaa gacagtggct attcagtcat
    26401 ttgaagattt aggatatttt acaatagata atatgccgcc tactctagtg cctaagtttt
    26461 tagaattagc agcacaatca ggagatacaa gcaagattgc tatggtagtt gacatgcgta
    26521 gtcgtctttt ctttagagaa attaactcta ttttggatag ccttgaaatt aatgataata
    26581 ttaactttaa gattctattt ttagatgcta ctgacaccga gctagtgtca cgatataaag
    26641 agacacgtcg tagccaccct cttgctgccg acggcagagt gttagatggt atttctctag
    26701 agagagaact attagcaccg cttaaaagta tgagtcagaa cgttgtggac actagtgaat
    26761 taacaccaag acaactacgt aaggtcattt ctaaagaatt ctcaaatcaa gatagtcaaa
    26821 gttcttttcg tatcgaagtt atgagttttg gttttaaata tggtatacct ttggatgcgg
    26881 atttagtatt cgatgtgcgt tttttaccaa atccatacta taaaccagaa ttaagagata
    26941 agacaggttt agatactgag gtttatgact atgtcatgag ttttgatgaa tcagatgatt
    27001 tttatgacca tcttcttgcc ttaataaagc caatcttacc aggctatcaa aatgaaggga
    27061 aatctgtttt gactgtggca atcggatgta caggtgggca acatcgtagc accgcttttg
    27121 ctcatagatt atcagaagat ttaaaagcag attggacagt taatgaaagc catcgtgata
    27181 aaaataagcg taaggagacg gtaaatcgtt catgagaaaa cctaaaataa ccgttatcgg
    27241 cggaggaacg ggaattccag ttattttgaa aagtcttcgc ttagaagatg ttgagattac
    27301 agcggttgtc acggttgcag atgatggtgg ttcgtcaggt gagttgcgct cagttatgca
    27361 actaacacca ccaggagatc taagaaatgt tttggttgcc ttgagtgata tgccgaagtt
    27421 ttacgaacaa attttccaat atcgttttgc agaaggtgat ggggattttg caggtcatcc
    27481 tttaggaaat cttattattg ctggtgttgc tgaaatgcaa ggttcaactt ataacgctat
    27541 gcagagttta acacagttct ttcatacaac aggaaagata tatccatcta gtgaacatcc
    27601 gctcactctg catgctgttt ttaaagatgg ccatgaagtg gttggagaga gtcaaatagc
    27661 cgattataaa ggcatgattg accatgtgta tgtcactaat acttataatg aagagactcc
    27721 tacggccagc cgcaaagttg ttgatgcaat tttagaaagt gatatgattg tgttggggcc
    27781 agggtcactt ttcacctcta ttttacctaa cctagttatt ccagaaatta aacaagctct
    27841 tttagaaact cgggcagagg tagcttatgt atgcaatatt atgacacaac gtggtgaaac
    27901 tgagcatttt acagacgcag accacgttga agtattgaaa cgtcatttag gacaagatgc
    27961 tatcgataca gttttggtta atattgaaaa agtgccagag tcttatatgg aaaacaatca
    28021 ttttgatgag tatttggtac aggttgaaca tgatttttca gggcttagaa aacatgccag
    28081 acgtgtcatc tcgtctaact ttttaaaatt agaaaaaggt ggagccttcc atcatggtga
    28141 cttcgttgtt gaggaactaa tgaatttagt aggaacacat ttatgagttt tacagtaaaa
    28201 gtcaaagaag agctgctagg tcataagagt gaaaataaga tggaactgtc agctattatc
    28261 aaaatgtcag gtagtttagg attagctaac cacggcctta acctttctat tactacagaa
    28321 aatgctaaaa ttgctcgtca tatttatagc atgctagaag aacattatca tttgcagcca
    28381 gaaatcaaat accatcaaaa aacgaactta cgaaagaatc gtgtgtatac tgtttttatt
    28441 gaagaaaaag ttgatgttat tcttgctgat ttgaagttgg ctgatgcttt ttttggtatt
    28501 gaaactggta ttgaacactc tatattggac aatgatgaaa atggaagagc ttatctcagg
    28561 ggagcattct tatcaacggg tactgttaga gaacctgatt ctgggaaata tcaactagaa
    28621 attttttctg tttatctaga ccatgcacaa gacttagcta atcttatgaa aaaatttatg
    28681 ttagatgcaa aggttattga acataaacat ggagcagtaa cttatttgca aaaagcagaa
    28741 gatattatgg atttcttgat agtcattgat gccatggaag ctagagatgc ttttgaagag
    28801 attaaaatga tacgtgagac acgtaatgat attaatcgtg ctaacaacgt tgaaacagct
    28861 aatattgcta gaacaatcac agctagtatg aagactatca ataatattat caagattatg
    28921 gatactattg gttttgatgc actgcctagt gatttaagac aagtagctca ggtgcgtgtg
    28981 gctcatccag attactctat tcaacaaata gcagacagtt tagaaacgcc tctgagtaag
    29041 agtggcgtta accatcgctt acggaaaata aataaaattg cagatgaatt atagaaggag
    29101 atattcacat ggcttgtaca acaatattgg ttggtaaaaa ggcttcttat gatggttcga
    29161 ctatgatcgc tagaacggaa gactctgtta atggcgattt cacacccaaa aaattaaagg
    29221 taatgatatc taaagatcaa ccgcgtcatt acaaatcagt tttatcaaat tttgaagtag
    29281 atttaccaga taacccactt ccttatactt cagtaccgga cgcattggga aaagatggta
    29341 tatggggtga agccggtatt aacagtaaaa atgtagcgat gagtgctaca gaaactatta
    29401 caacgaattc ccgcgttttg ggtgcagatc ctttggtttc agatggtata ggggaagagg
    29461 atatactcac tttagtgctt ccctatattc agtcagcgcg agaaggtgtg gagcgtttag
    29521 gtgctatttt ggaaaaatat ggaacctatg aatcaaatgg tattgctttt tcagataccg
    29581 aagaaatatg gtggttagaa acaattggtg ggcatcattg gattgctcgt cgcgtacctg
    29641 atgatgttta tgttactaat cctaaccaac taggaattga tcattttgaa tttaataact
    29701 gtgatgacta catgtgctct agtgatttga aagagtttat cgaacaatac catttagatt
    29761 tgacctattc taatgagcat ttcaatcctc gatatgcttt tggtagccaa cgtgataaag
    29821 atcgtcatta caacacacca agaagttggg caatgcagcg ttttttaaat cctgaaattg
    29881 aacaggatcc acgtagcttg tttattccct ggtgtcaaaa gccttaccga aaaattactg
    29941 ttgaggatat taaatatgtg ttgagtgatc attatcaaga cagtgtgtat gacccatatg
    30001 gaccagaagg ggatgcggta agtaggagag cttttcgttc agttggtatc aaccgaacta
    30061 gtcaaacgtc tattctacaa ttacgaccaa ataaatcact tgaaacgaca ggtgttcaat
    30121 ggttatctta tggctctatg ccatttgcaa ccatggtgcc gttgtttaca caagttgaga
    30181 ctgtaccaaa ctatttttcg aatacaacca aggatgcttc aacagataat ttttattgga
    30241 ccaatcgttt aattgcagct ctagcagatc cacactttta tcaacatgaa gctgatattg
    30301 aaagctatat cgagagaacg atggctcaag gacatgcaca tattaacggt gttgatagag
    30361 aagttgctga gaataaagag attgattttc aacagaaaaa tcaagaaatg agtgactata
    30421 tccaaaaaga aagccaagaa ttgttaaatc gtattctatt tgatgcaagt aatttaatga
    30481 caaatcgctt ttcaatggga gattaaaaaa ctcggaatat tttccgggtt ttttaagata
    30541 aaaacgcttg cataagtata taaaacttgc aaaaaaaaaa tatcgtgcta tactttacta
    30601 gtaaaatatt aaccggtaaa taacgggtta aataaaaagg agaaaaaatg agaaagaaat
    30661 ttcttttatt gatgagcttt gtagctatgt ttgcagcttg gcaacttgtt caagttaaac
    30721 aagtttgggc tgatagtaaa cttaaagtgg taacaacttt ttacccagtt tatgagttta
    30781 caaaaaatgt cgttggtgat aaagctgatg tatctatgtt aattaaagca ggtacagaac
    30841 cgcatgattt tgaaccatca actaaaaaca tcgctgccat ccaagattca aatgcttttg
    30901 tttacatgga tgataacatg gaaacttggg ctccaaaagt agctaagtca gttaaatcca
    30961 aaaaagtaac aactattaaa ggtactggcg atatgttact tactaaaggc gtcgaagaag
    31021 aaggtgaaga acatgaagga catggtcatg aagggcatca tcatgaactt gacccacacg
    31081 tatggttgtc tccagaacgt gcgatttctg ttgtagaaaa catccgtaat aaatttgtca
    31141 aagcttatcc aaaagatgca gcttcattta acaaaaatgc agatgcttac attgcaaaat
    31201 taaaagagct tgacaaagaa tacaaaaatg gtttgtcaaa tgctaaacaa aagagttttg
    31261 tgactcaaca cgcagcgttt ggttacatgg cgcttgatta cggtttaaat caagttccaa
    31321 ttgctggtct tactccagat gcagaacctt catcaaaacg tttaggcgaa ttagctaaat
    31381 acatcaagaa atataacatc aactacattt attttgaaga aaatgcttca aataaagttg
    31441 ctaaaacttt agcagatgaa gttggcgtga aaacagctgt gcttagtcca cttgaaggac
    31501 tttctaaaaa agaaatggca gctggcgaag attacttctc agttatgaga cgtaatttga
    31561 aagttcttaa aaagacaaca gatgttgcag gtaaagaagt agctcctgaa gaagataaaa
    31621 ttaaaacagt tgaaacaggt tactttaaaa ctaaagatgt taaagaccgt aaattgacag
    31681 attactctgg taattggcaa tcagtatatc ctcttcttca agatgggaca cttgatccag
    31741 tttgggatta caaagctaaa tctaaaaaag atatgactgc tgcagagtac aaaaaatatt
    31801 atacagcagg ttacaagact gacgtagaat caatcaagat tgatggtaaa aaacatcaaa
    31861 tgacctttgt acgtaatggt aaatcacaag catttacata caaatatgca ggttacaaaa
    31921 tcttaactta taaaaaaggt aatcgtggag tacgttatct ctttgaagct aaagaaaaag
    31981 atgctggtca attcaaatat atccaattta gtgaccatgg tattaaaccg aataaagctg
    32041 aacacttcca tatcttctgg ggttcagaaa gccaagaaaa attatttgag gaaatggaaa
    32101 actggccaac atacttccca gctaaaatgt ctggacgtga agttgcccaa gaccttatgt
    32161 ctcattaata tttaaaagat gatatcggat acgatatcat ctttttttct ttgtacgtga
    32221 taggctcaaa catagaaaaa agcacttcta atagaagtgc ttttaaaatt aatgctatgc
    32281 ttattgagca gcgtttgcgt ctttgagacc atattttttg ttgaaacggt ccacacgtcc
    32341 atctgcttga gtgaattttt gacgtcctgt atagaatgga tgtgaatctg atgagatttc
    32401 cacacgaata agtgggtaag tttcaccttc gaattctact gtttctttag ttgatttagt
    32461 tgaaccacta aggaatttgt aacctgtagt tgtatctaag aaaacaactg gacgataatc
    32521 tggatggatg tcttttttca tctttaaaaa tttcctttct gccataactc gtctgagtca
    32581 tagttattaa ccactctatt ctatcaaata agaatagtct tgacaagtgt tttgataaaa
    32641 aaagtaaaat tataaagaaa gtatttcctg gatttcttgg taaatagctt gattctcttc
    32701 caaactataa gaattagctc cgctggctaa aggatgtccg ccaccattat gacgtttagc
    32761 tatttcatta atgatgtgtg acttactacg taggcgcaca cgataatgcc catcgctttg
    32821 ttcaacgaaa attgcccaac tttctacaat atcaattttt ccaggtacac caacgattgc
    32881 agcagtctct gcatctgtta catcgaagcg ttttaggtct tcttgtgtta aagtaacgca
    32941 tgcagcgccg ttcttgtcaa ttttaagttg ctcaaaaata aagccttgta attttgcaat
    33001 tttgaaagga aaggaatcca tttgacgtgc cattgcagaa aaatcaaagt caaattcacg
    33061 aagtttgcta gcaatcttca gtgttttact tgtggtagca ggatataaaa agcgtcctgt
    33121 atctcctaca ataccattat atagaactct tgctgcactt gttgataaca acaaatcaca
    33181 agaaagagca aagtctgtca caatttcact tgcactagag gcgttagtat ctacataaga
    33241 gagatctcca tagacttcat cgtttggatg atggtcaatt ttaattaaaa agtcaccttt
    33301 tttataacgt tcatcatcaa tgcgtggggt atttgctgta tctgtaacaa caacaagagc
    33361 gccttgatag tcttggtcgg tcacttgatc cattttagca atccaagcga gagtgggttc
    33421 gtcaaaacca gtagcaagta cttttttctt tggaaaatta tgcctgatga tatcccttaa
    33481 gccgatttgg ctaccaagag catcagggtc tggtctcata tgtcgatgga taatgatggt
    33541 gtcgtactct ttgattttat caagaatttg ttgaaaaata atcatttttt acctacctaa
    33601 ctgttttgtc ttttagtgaa gaacaaggct atattgttga ctatattata gcttaaatct
    33661 tttattgagg aaagaaatta agatagcggt cacaatgttt gtttattttt atatgatggt
    33721 atgttatttt tttataaaat tcggatactg ctaactaata atctttttta taataatatt
    33781 ttgtaaatgt attgcttttt agctaacttt tgttaatata gtaaaaatgc tttttaatag
    33841 gagataaatt atgacacaag cagtattaaa agaattggct aaggcggagt tgcattgtca
    33901 tctagatggc tcactttctt taccagctat tagaaaattg gctaatatgg ctgatataat
    33961 actgccgagt agtgataaag aattaagaaa atatgttata gctccagctc aaacagaaag
    34021 cttggtagat taccttaaaa cgtttgagtt tatcagaccg cttcttcaaa cgaaggaagc
    34081 attacgtttt gctgcttatg atgttgctag acaggcagct ttagaaaatg ttatttatat
    34141 tgagattcgt tttgcgccag aactctctat ggataaaggt ttaactgcaa gtgatacagt
    34201 ccttgcagtc ttagaaggtt tagcagacgc tcagaaagaa tttaatatcg ttgcacgcgc
    34261 ccttgtttgc ggtatgcgcc aatcgtctca taaaacgacc aaggacatta ttaaacacat
    34321 tgttgatttg gctcccaaag gcctggtagg ttttgatttt gcaggtgatg aattttccta
    34381 tccaacagat agtttagtag atcttattca ggaagtaaaa cgctcaggtt acccgatgac
    34441 tttacatgca ggtgagtgtg gttgtgctaa gcatattgcg gatagtttaa atttagggat
    34501 taagcgtatg ggacatgtga cagctttaac aggacagagg gatttaataa agcgatttgt
    34561 cgaagaagat gctgtagcag agatgtgttt aactagcaat ttacaaacaa aagcagcctc
    34621 aagtattcaa agttttcctt atcaagaact ttatgatgct ggaggtaaga ttacaattaa
    34681 tacagataat cgtactgttt ccgatacaaa tttgacgaaa gaatatagtc ttttcgtaac
    34741 ttattttgga acgaaaatag aagactttct tgttttcaat caaaacgctg tgaaggcctc
    34801 ctttacaagt gattcagaga aagatacttt actacataag ctacaagaaa actatgacag
    34861 ctatctaaaa taaccttcac gattctttaa aaatatgata gaatagtggt aatttacaag
    34921 tggaggaaga tatggcttta gcaaagattg tttatgctag catgaccgga aatactgaag
    34981 aaattgctga cattgttgct gataaattac gtgacttggg tctagatgtt gaagtagaag
    35041 agtgtacgat ggttgatgct gcagattttg aagatgctga cattgcaatt gttgccactt
    35101 atacatatgg tgatggagat ttaccagatg aaatcgtgga cttttatgaa gatttagcag
    35161 aggttgattt atctggtaaa gtttacggcg tagtgggttc aggtgatact ttctatgatt
    35221 acttctgcaa atctgttgat gagttcgagg ctcaattcgc attaacagga gcacaaaaag
    35281 gtgctgattg tgttaaagtt gatttagcgg cagaagacga agacattgaa aacttagaag
    35341 cttttgctga agaaattgct tctaaactta actaagctac caaaaagacg aacacgagtc
    35401 gtctttttaa cttttttacc atcttttgta actagaatat gagatataga tatggaatta
    35461 gaagagattc gtcaagaaat agatgaaata gatcagcaat tagtaagcct tttagaaact
    35521 agaatgggtc taattttaga ggtcatagca tttaaaaaga aacatcgact tccagtttta
    35581 gataataatc gtgaaaacga agtgcttaac aatgtattga agaaagtcca aaaccatcaa
    35641 tttgacgatg tgatacgagc aactttcaaa gacattatga cagaatctcg tgtttatcaa
    35701 aaggagaata ttgtagacgg tgattaaacg atttgataat tatgctcagt acgtacttgt
    35761 tttaggtttt agcttactaa ttggtggtgt ggttggggct attacagcag tctttggacg
    35821 tgttctatta ttcctgacag cctttagaag tgattatata gcttacctac tcccttttct
    35881 tagcattgtt ggtttattta tagtttttgt ctatcaaaaa tttgggggta agagtgttaa
    35941 agggatggga ttggtatttg aagtaggtca tggtaatgag gagactattc cgaaacgtct
    36001 tgttcctctc gtgatattaa caacttggtt aacccactta tttggtggta gtgcaggtcg
    36061 tgagggggtt gctgtacaga ttggggctac cgtttcacac tactttcaaa aatattgccg
    36121 tttacaaaac gcttctcaac ttttcctagt aatgggaatg gcagcaggtt ttgctggttt
    36181 atttcagact cctttagctg ctactttttt tgcgattgaa gttttggtag taggtcgttt
    36241 aatggtttcg tatgtattac caagtttaat tgctgccctg acagcaaatt tcgtctcaca
    36301 ttctttaggt ttagaaaaat tttctcatag tatagcaaca tcaatggcgt taacaccaga
    36361 tattattcta aaattattgg ttttaggact atgttttggt ctgtgtggta atttatttgc
    36421 ttatttattg gctaaagcta aattaattgc ttctagtagg ttgctaaacc catataaacg
    36481 gattttcaca cttggattgt tagctacttt tttattgttt atcttccatt ttggacgata
    36541 ttcaggactg ggaacaaatc taattgaggc tagcttcact aataagaacc tttatgatta
    36601 tgattggttg ttaaaattgt gtttgacagt tattactttg gctgcaggtt atcagggagg
    36661 agaagtaact cccttatttg caatcggagc aagtttagga gtgattattg cgcccatttt
    36721 aggtttgcca gttattttag ttgctgcttt aggctatact agcgtttttg ggagtgcaac
    36781 gaatactctg ttaggtccta tcttaatagg aggcgaagtc tttggctttg ccaacactcc
    36841 ttattttgtt attgtatgtc ttgttgctta ctccatatca catgctcata cgatttatgg
    36901 tgctcaatct agatagttag tgatgagttt taaaagagtt tatcagtttg tagaacggaa
    36961 tattccgttc tcttttttat tctcttaaag tttataaaaa tggagtttgt caattttatg
    37021 aatgcaaaaa ttatgtggct aatataattt cttcaaagaa attgatttta tgattgtcat
    37081 taaaatactg taaaatccaa ttgaatgtgt aggaacttag gttaggataa tgtctacaag
    37141 catttaagtc gcgatggtga aatgcattat aagataaaaa gttgatgtgg atttcaactt
    37201 tatgggaaag agttaagtga cacgaaagct aatatagaaa acgtagctta aaaaatcaaa
    37261 tattaaatga attaaactct caaaaatcag aaaaaagcta acctagaaac agtaaatcaa
    37321 atttaaataa tatacaaata actattctaa aggatgacac cttgttcttc tagataaaat
    37381 agctttacaa gtaggttaaa atttggtaga attgtgagag tgtgtgatgg acgcacaaaa
    37441 aacggctaat ccgctgagac aagtacttaa gattagtaag agaaggagaa taaaaatgaa
    37501 tccattaatt caaagtttga cagaaggtca acttcgttct gatatccctg agttccgtgc
    37561 tggtgatact gtacgtgttc acgctaaagt tgtcgaaggt actcgcgaac gtattcagat
    37621 ctttgaaggt gttgttatct cacgtaaagg tcaaggaatc tcagaaatgt acacagtacg
    37681 taaaatttct ggtggtatcg gtgtagagcg tacattccca attcacactc ctcgtgttga
    37741 taaaatcgaa gttgttcgtt atggtaaagt acgtcgtgct aaactttact acttacgcgc
    37801 attgcaaggt aaagctgcac gtattaaaga aatccgtcgt taattttgat gatcagattt
    37861 taaaaatgct tggttgtttg aggatagtaa ctatgtttta aaactggaca accaagacgt
    37921 aaaaaatctg cctgtgggca gtttttttac taggtcccct tagttcaatg gatataacaa
    37981 ctccctccta aggagtaatt gctggttcga ttccggcagg ggacatattc attgcatgta
    38041 aatagcggtt tagagctatt ttgccccaaa tccgccccaa atttctctga ttaagtttat
    38101 cgttcctatc tttttgttct tgtaattgat gtgcgtaaac ttctaaagtg atatttaaat
    38161 tctcgtgatc taaaacttga gagatggaaa ttagatagct tgcaaatgta tgcctgagag
    38221 agtgcactcg tacctcgcga ccagttattt ttcggatagt tttattgact gcattatttg
    38281 aaagtttgtc gaataatctg tcgtttttat tttttgtaaa ttcatgcaaa aaaaataatg
    38341 tatcattgtc aattggtata tttctgatac tacttttgtt ttttgttggc aggtatcttt
    38401 ggttgaaatg ataatcccaa gttttattaa ttgataaata tttgttagtg taatcaatat
    38461 cattaactgt taaacctaaa cattcagcga agcgcatgcc agttttagcg atgaggtata
    38521 acgctgcata cgattgatgt tgtgattttt ctttacaaat ttttatcaag cgtaagtatt
    38581 cattggtttc aagaaatttt atctctattt acgcccctta ttttttgctt taaccttagt
    38641 gaataaacaa aaattttttt ctatatatcc ctcgtgaaca gccatggata cgcaggcttt
    38701 tacatgtatg ttaaaacgct ttactgtatc ttgcacatgc gtttgactat aatgatttat
    38761 gacttgttga tatttagtgg aagtaatatt gcaaagtaat atatttccta ttatatgttt
    38821 atacgatatt cgatattccc acccgttgtc gcgtttacgg aaatacgcca ttgatatact
    38881 ccacattagc taaagaacag ggtgttcaag gctaccttga tggaaaaggc tctcttagag
    38941 atatttgtaa atggtatgat atctcaagtc gctctgttct ccaaaagtgg ataaaacggt
    39001 atactagtgg tgaagacttg aaagccacta gtagaggata tagccgtatg aaacaaggaa
    39061 ggcaagccac atttgaagaa cgtgtagaga ttgttaacta caccattgcc catgggaaag
    39121 actatcaagc agctattgag aagtttggtg tttcctacca acaaatttat tcttgggtgc
    39181 gtaagcttga gaagaatggc tcacaaggtt tggttgatag acgtgtgaaa gggttggaga
    39241 gtaggcctga tttaaccgag attgagcaac tttaactcaa gattaaacaa ttgcaggaac
    39301 gtaatcgtct cttagaaatc gaggttagtt tactaaaaaa gttagaagac atcaaacgag
    39361 gaaacagacg gtaagactag gtaagcattt agcggagttc caagtaatca agaattatta
    39421 cgatgaggaa tctaatgtgc ctattcaggc cttatgccaa ctcttgaagg ggtctcgttc
    39481 aggctattac aagtggctca atcgtcaaaa aacagatttt gagacaaaaa atacaaagct
    39541 aatggctaaa atcaaggaac ttcatagact ctacaatggt atcttaggtt atcgccgtat
    39601 gacaacattt attaatcgtc aacttgggac aacttaaaac aagaaacgga ttcgttgatt
    39661 gatgaacatt ctggggatta gttcagtcat tcgtcgtgtt agccatgctt gtacaaaagc
    39721 tggtgacaga ttttacgaag aaaatattct taatcgtgaa tttacagcca cagctcataa
    39781 ccagaaatgg tgcacagatg tcacctatct ccaatacggt ctgggagcta aagcttatct
    39841 cagtgcgatt aaagacctgt ataacggttc tattatcgct tatgagatta gtcacaacaa
    39901 tgaaatccac ttgttatgaa gaccattaaa aaggggctag agctcaatcc aggagccaca
    39961 cctatcatcc atagcgattg aggtagtcaa tatacttcca aagaataccg ttatatcata
    40021 caacaagctg gtctgacctt atccatgtcc cggattggca aatgtattga taatgcacca
    40081 actgaaagtt tctttgggtt tttcaagact gagtcttacc accttaagaa atacaactct
    40141 tatgatgagt tggtcaatga tgtggcacgt tatatcgaat tctacaacac acaacgttat
    40201 caatcaaaat taaacaacct gactcctcta gaattcagga atcaggttgc ataacttatc
    40261 ttttattatt tgactgtcta cttgacaggg agccgttcag attgcttaac ctttctaaat
    40321 ttgctaaaat agctacaaga aaacgagcca tttaatgctt atttcttata ctgtcttgcc
    40381 tcacgctctc ctcgaccaaa aattgagcgt gaggcttttt gtttcattaa acgatgatat
    40441 ttccatattc atcagtttgt tttccgagag ccatcaaagc ttcgataagg tcgataattc
    40501 caggaataaa ggtaatacta aaaataatat ataaaaaaac ctggcctatt tttcctgcgt
    40561 aaaatttatg cgctccaatg ccgcccaaaa gaacgttaat aaaacataaa
//
LOCUS       DATRSD010000006        40427 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRSD010000006 DATRSD010000000
VERSION     DATRSD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40427
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.006"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..2018)
                     /locus_tag="VBD85_000263"
     CDS             complement(<1..2018)
                     /locus_tag="VBD85_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO7132840.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFIKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALLTGYFSLTKASLKLALAIMGGFLIGLLFAFLTRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELTRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSCF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLQNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYS"
     gene            complement(2020..2256)
                     /locus_tag="VBD85_000264"
     CDS             complement(2020..2256)
                     /locus_tag="VBD85_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132841.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHSSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            2423..3376
                     /locus_tag="VBD85_000265"
     CDS             2423..3376
                     /locus_tag="VBD85_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO7132842.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQTWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFLTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            complement(3426..5321)
                     /locus_tag="VBD85_000266"
     CDS             complement(3426..5321)
                     /locus_tag="VBD85_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO7132843.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELF
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFE
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            5469..6452
                     /locus_tag="VBD85_000267"
     CDS             5469..6452
                     /locus_tag="VBD85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7132844.1"
                     /translation="MIDKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPKVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEALDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTKDGHQDIIEAGK
                     TENALQYEVADMEEAVSGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            complement(6498..7661)
                     /locus_tag="VBD85_000268"
     CDS             complement(6498..7661)
                     /locus_tag="VBD85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7132845.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKGTNDKIKTKGQKVPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFFKCLFFKKM"
     gene            7773..8417
                     /locus_tag="VBD85_000269"
     CDS             7773..8417
                     /locus_tag="VBD85_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO7132846.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQPVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDLTVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGY
                     FHLELSSLADSFGVSYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            8483..9097
                     /locus_tag="VBD85_000270"
     CDS             8483..9097
                     /locus_tag="VBD85_000270"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7132847.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(9169..10170)
                     /locus_tag="VBD85_000271"
     CDS             complement(9169..10170)
                     /locus_tag="VBD85_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7132848.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(10250..11260)
                     /locus_tag="VBD85_000272"
     CDS             complement(10250..11260)
                     /locus_tag="VBD85_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HEO7132849.1"
                     /translation="MFFSVTETKSFSFDNVNLETLLLFGKPSIYLFEEIPRATIGESA
                     YLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDAGRYTYKEE
                     QLRYDFKRSTSHSTCTLDGESLEMIKDSWTYNSYPKCDYCQLTSKDRYHLVEGQLHVQ
                     RASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGLVNDLKLVS
                     PTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCVPLVQTNSH
                     KELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDKNNGKMSRL
                     KN"
     gene            11175..11510
                     /locus_tag="VBD85_000273"
     CDS             11175..11510
                     /locus_tag="VBD85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132850.1"
                     /translation="MDGFPNKSKVSKLTLSKENDFVSVTEKNNLIDKYKELLSKIPEN
                     TQNPGEKNIRNYLEKEYEELLQKDRLFKHEYTEFTKSLNLNETFYSQLKEGEMKLSEN
                     PEKGETNTN"
     gene            complement(11682..12296)
                     /locus_tag="VBD85_000274"
     CDS             complement(11682..12296)
                     /locus_tag="VBD85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7132851.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(12429..12854)
                     /locus_tag="VBD85_000275"
     CDS             complement(12429..12854)
                     /locus_tag="VBD85_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7132852.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(12981..17387)
                     /locus_tag="VBD85_000276"
     CDS             complement(12981..17387)
                     /locus_tag="VBD85_000276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO7132853.1"
                     /translation="MSELFKKLMDQIEMPLEIKNLSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQDLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(17510..18094)
                     /locus_tag="VBD85_000277"
     CDS             complement(17510..18094)
                     /locus_tag="VBD85_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO7132854.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASAWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(18219..20072)
                     /locus_tag="VBD85_000278"
     CDS             complement(18219..20072)
                     /locus_tag="VBD85_000278"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO7132855.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDKNQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFSRGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(20164..21423)
                     /gene="rseP"
                     /locus_tag="VBD85_000279"
     CDS             complement(20164..21423)
                     /gene="rseP"
                     /locus_tag="VBD85_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO7132856.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(21454..22248)
                     /locus_tag="VBD85_000280"
     CDS             complement(21454..22248)
                     /locus_tag="VBD85_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO7132857.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAIIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(22263..23015)
                     /locus_tag="VBD85_000281"
     CDS             complement(22263..23015)
                     /locus_tag="VBD85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO7132858.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(23181..23522)
                     /gene="yajC"
                     /locus_tag="VBD85_000282"
     CDS             complement(23181..23522)
                     /gene="yajC"
                     /locus_tag="VBD85_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO7132859.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(23599..23943)
                     /locus_tag="VBD85_000283"
     CDS             complement(23599..23943)
                     /locus_tag="VBD85_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7132860.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(24134..25297)
                     /locus_tag="VBD85_000284"
     CDS             complement(24134..25297)
                     /locus_tag="VBD85_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO7132861.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(25322..26659)
                     /locus_tag="VBD85_000285"
     CDS             complement(25322..26659)
                     /locus_tag="VBD85_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO7132862.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGSAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPSETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            26813..28339
                     /locus_tag="VBD85_000286"
     CDS             26813..28339
                     /locus_tag="VBD85_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7132863.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQNHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGS
                     "
     gene            28341..29021
                     /locus_tag="VBD85_000287"
     CDS             28341..29021
                     /locus_tag="VBD85_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7132864.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(29042..30037)
                     /gene="galE"
                     /locus_tag="VBD85_000288"
     CDS             complement(29042..30037)
                     /gene="galE"
                     /locus_tag="VBD85_000288"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7132865.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(30125..31732)
                     /locus_tag="VBD85_000289"
     CDS             complement(30125..31732)
                     /locus_tag="VBD85_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO7132866.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(31861..32994)
                     /gene="ugpC"
                     /locus_tag="VBD85_000290"
     CDS             complement(31861..32994)
                     /gene="ugpC"
                     /locus_tag="VBD85_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO7132867.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(33095..33946)
                     /locus_tag="VBD85_000291"
     CDS             complement(33095..33946)
                     /locus_tag="VBD85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7132868.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTPSLEDASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVEKIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(34041..34937)
                     /locus_tag="VBD85_000292"
     CDS             complement(34041..34937)
                     /locus_tag="VBD85_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO7132869.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGDPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     KEMLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNHALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(34991..35968)
                     /gene="lacD"
                     /locus_tag="VBD85_000293"
     CDS             complement(34991..35968)
                     /gene="lacD"
                     /locus_tag="VBD85_000293"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO7132870.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(35970..36902)
                     /locus_tag="VBD85_000294"
     CDS             complement(35970..36902)
                     /locus_tag="VBD85_000294"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO7132871.1"
                     /translation="MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTR
                     VLSQLGDNVVATGLLGGYFGDFIRSGLDALEIRHQFLSIGGETRHCIAVLHEGQQTEI
                     LEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKV
                     VLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDIFRGIDW
                     VIVSLGAKGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDTNLLKR
                     ANVLGMLNAQETLTGHINLTYYQELISQIQVKEV"
     gene            complement(36913..37428)
                     /gene="lacB"
                     /locus_tag="VBD85_000295"
     CDS             complement(36913..37428)
                     /gene="lacB"
                     /locus_tag="VBD85_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO7132872.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(37449..37874)
                     /gene="lacA"
                     /locus_tag="VBD85_000296"
     CDS             complement(37449..37874)
                     /gene="lacA"
                     /locus_tag="VBD85_000296"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEO7132873.1"
                     /translation="MTIIIGADAHGVELKEVIRQHLTSLGKEIIDLTDTSKDFVDNTL
                     AIVAKVNQKEDNLGIMVDAYGVGPFMVATKVKGMIAAEVSDERSAYMTRAHNNARMIT
                     LGSEIVGPGVAKHIVEGFVDGTYDAGRHQIRVDMLNKMC"
     gene            complement(38122..40427)
                     /locus_tag="VBD85_000297"
                     /pseudo
     CDS             complement(38122..40427)
                     /locus_tag="VBD85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
BASE COUNT        13021 a         8158 c         6310 g        12938 t
ORIGIN      
        1 aatgaatagc tttctaattc attgacatta tcccttaaaa tttgagactc tttagaacta
       61 attaaaccgt ccctttcaaa ttcagagatt gttctgcgtt ccaagtaata tcctcttagg
      121 gtttcattta aaatatctgg cgttatgcgt gcaatgacac gttctacaaa taaacctgat
      181 ttaattaatt ctgcgtctcg aatgcgttca ttttgtaaga aatctatcaa agttgcatta
      241 taataacctt ccatatcttc tagcgtttct aaaatgagat cattatttga taagtacaaa
      301 tctgttaagt gctgacgatt gacttctgct agcaaccgtt tcctattttt gaaatgtttt
      361 ttccgatttc gtattccttg tccaaatgtt aaaaactcat gaacaatacc acgaataaca
      421 cgcataagaa tcgttaaact ataagtgata tttgacacaa agctacgatt aacttgacgt
      481 tctaagtatt ttaaataacg ctggtaaata cgatattctt tgatagaaat attgccataa
      541 cgataagcag cttcaagccc gtcgctttct atactaagca tcatcagttg caattcagct
      601 aagtccttct taatgtcact agattcttgt tctaaaatga gatgacgaat acggctatta
      661 taattatcta taactgcata aaatgaagct tgattttcag aactcttacc gtcttcttct
      721 aagacagata atacttttgt taaaatcgat agacgtatta aataatcatc tttcacctgt
      781 aagggaggcg ctaactttgg taatactaaa agtcccgtta aaaaactcat tagtgttact
      841 gcaccaacag tgaaaagaat caaactatac cccaactctc ctatgactga gtgcttgggt
      901 aataagagta ttgttgctat ggaaacactt cctttaactc ctgaaaaagt caacagttga
      961 atctctctcc aatacttttt aaatgattta tggtatttga aagatctcca agcataaaag
     1021 caagaaactg ctagaaagcg aactagaaaa agtgtgcaag taactaaaac aacaatacta
     1081 ataagatcaa aattactata agcgttactt gttaaaagag gaccaatgat acgtgtcagt
     1141 tctcttccaa atattaagaa aactaaacca tttaaaataa aatttaaggt ttcccaaata
     1201 acagaggtaa cgcgatctac ttgagcatca aagagagtca cttttttcaa acgatttgct
     1261 tgcattactc ctgccactac caccgcaata atagctgaaa atcccaacaa atcagcaacg
     1321 aaataagcaa caaaaggtaa ggttaattcc aaaagtagag cccctgtaac atcagcagca
     1381 tcaaatttct ctaggacagt gagacataac ctcgttagaa aagcaaaaag caagccaatt
     1441 aaaaaaccac ccattatggc caatgctaac ttgagactag cttttgttaa ggaaaaataa
     1501 cctgttaaaa gggctgttaa agcaaactgg aaggaaacta gaccactagc gtcatttaaa
     1561 agaccttcca atttgaggat attctctgct cgtttaggaa attgaaacct cttagcaatc
     1621 gatataaagg caacggcatc tgttggacct aaggctgctc caagcgcaaa gcatgccgca
     1681 agtggtaaac taactggcaa aaggtgccct gctacataac caacgacaat agctgttaaa
     1741 aataccgttg gtaagattaa gtaaagaata atagtccgat actttataaa actaccaata
     1801 tcactttctt gaccttctct aaagttcaaa ggggcaataa caaaggcaag aaataactca
     1861 ggatcgatat ggacttgtga cttatgaaaa actaagccag agagaatccc aaagaccaac
     1921 tggataaatg gtaatggtaa tttaggaaat atcctattga taacattaga taaaatcaaa
     1981 aacaggagaa agctgactat tagaatagtt tcttccatat tattcctcct tcataacatc
     2041 tatcaattct ttcttcaatt tctcaatttt atcatcgatt tttttagtat cttctctctc
     2101 caatcgacag cgacatcttt ctttttcaag tattattaat ttcttctttt tcttctttac
     2161 tttacttgag tgaacatttg aaaattcttg atttttagaa gaaaggtatt tatcttttag
     2221 tcgattcaat cgttcttcca aagataattg agacatcata acctcctatt taataaccaa
     2281 attattaata ataagtattt tttctttcat cggctatctc tagtataaca cgcatttctt
     2341 aaattgatag aatttaagaa tatcgttgct cattatctcc atatgtggta gactaacaac
     2401 aatttaaata ggaggttaac caatgaaaaa attaacaaaa ggcagccaca ttcgtgttct
     2461 tagtccctct tcttccattg aacgtctggg tggttttgag gctaacctat tagccaaaga
     2521 agtactagaa aagctgggat ttcaagtgtc attctctaag cactatctag aaaatgatat
     2581 cctctattct gcttcgattg ctagccgcgt agaagattta catgaagcat ttgccgaccc
     2641 ttctgttgat gccatattag caaccattgg cggattcaat tccaacgagc ttttaccata
     2701 tttagattat gatttaatct ctaagaatcc caaaataatt tgtggttact cagattcaac
     2761 ggcattcctt aatgctattt ttgctaaagc aaaaatccaa acttacatgg gtcctgctta
     2821 ttcttctttc aaaatgaagg aaggtcaacc ttaccaaaca caaacttggc ttactgccat
     2881 gacagaaaac cactatgaac tttggcctag tgaagaatgg tctagtgatc cctggtatga
     2941 tcccagcaaa ccacgccaat ttttcctaac agaatggaaa atttataatc acggaaaagc
     3001 ttccggtacg attatcggag gtaatctctc tacgtttggc ctacttcgag gtactcctta
     3061 tgcaccgaaa attgaacgct atgtcctctt gattgaagag gctgaggaaa gtaattttta
     3121 tgaatttgac cgcaaccttg ctgcaatttt acaagcttat cctcatccac aagctattct
     3181 tatggggcgc tttcctaaag aatgcggcat gactccacaa gtctttgaat atatcctttc
     3241 aaaacatgct attttcaagg aaattcctgt tatttatgat atggattttg cgcataccca
     3301 acctcttttg acagtgacta ttggagctga gataagcgtt gacactacta cattatcttt
     3361 atctataaaa gaataaaaaa atactcctgc tactcatctg agtaacggga gtttatcatt
     3421 tagagttacc aaataataac acgtttttca actggacgcc acatgttatc accatcttta
     3481 acatcaaact ctttatggaa ttcttcaaaa ttcgttactg taatattagt tcgccattgt
     3541 gctggggcgt gaacatctac actagctaac atttgcatat actcatcacg tgccttcata
     3601 cgccaaattg tcgcaaagtt aataaagaaa tccctcgctg aaaaatctga ctcacgctgc
     3661 gcagcttcta aagcacaagc aactccacct aaatctgcaa cattttctga tactgttagt
     3721 ttaccattga cttttgcgcc ataagattct agtccatcaa actgctcaac tactttatct
     3781 gtaagttttt tgaaagcttc aaaatcttca tcagtccacc aattattcaa acttccatgt
     3841 tcatcaaatg aagctccatt agtgtcaaaa gcgtgtgata tttcatgtgc aatcactgca
     3901 ccaattcccc cataatttgc tgatgaactt tgctctaaag cataaaatgg tgcttgtaaa
     3961 atggctgctg ggaagacaat ttggttttgt tgtggatcat aatacgcatt gaccatgtgg
     4021 gcaggcatat gccattcact tctatcaact ggtttattcc atttactcca accataggct
     4081 attgaaattt tgtctaaatt agtagcattt tcgacaagcg atagtttagg atcaatgatt
     4141 tttttagtat aagtctctgg tagtttctca gggtatccaa tgtgtggcgt gataacatta
     4201 agtttcacaa tagctttttc tcgcgttgac tgtgcaagcc aatccgcctt ttctaagcgt
     4261 gacttataga catcaatcat cgttgcaatc ttatgctcaa catcagcttt agcttctgga
     4321 gagaattttt cccctgcata ccacaaacct agagcttgat tgtaaggtcc tgaagcaagg
     4381 tagtaagctg cttttttctt atccattgct tgtggtgtgc ctgataaagc tcgactgtaa
     4441 actcccgatt tgatacgaat atcatcagtt aagtaagcat tataggcatt cgcagctgat
     4501 aaaataagat ctgcctttaa taattcccaa ttagattctg agtaatagtc agatgcaaat
     4561 tctgtccaga aacgttcttc aggaacaata actttatctg gtttttgtcc taatatcttt
     4621 tggaaaatcg catctaatgg aagttcaggt gctaattttg tgaaatcttc ccagttataa
     4681 ggatggtaaa gtttaacata ttccgaactc tcttcgcgag ataagacata ttgtgccaat
     4741 tttgcatcca atgctaaaac cttatccaag atatctttaa tttcctgttc tgaaaggcca
     4801 aacaaaggca gtaagtcttc ttgactttta cgccaaagag caaagagttc ctttcctttt
     4861 tcattatctt cactataata agttgtatct ggtaaaataa tcccaggcgc ttcagcccag
     4921 agtacattca attgagcatt cataaagtca ggagccacac caaatggaaa ttcatttggt
     4981 ttaccttcta gttcatattc tgcaactttt gatgcaaact cagagaaaga ttgtaggcct
     5041 ttatattctt caattaatgg taatactggt gatactccta cctcttcacg tttttgataa
     5101 tcagctgtca ttttatgaaa agcaataaaa tgatttaaaa tcgtatcact tggtttattt
     5161 tcatcagcca gccacttatc tgtcgttgac agcattaatg cttcaatgtc atcagcaaga
     5221 tcagaaaaac cacctgtcct tggtttgtca tcaggaataa cagctgtctt agcccactcc
     5281 ccattaaccg cttggtaaaa atcatcttga tattttatca ttattttctc cttaatatct
     5341 catatgtaat ctcttacagt ctaacagaaa attcagagtt tgactattaa aaatactctc
     5401 atattggtgc tttctctaaa tatcattttg ttatttttgc tatactataa accaacaagg
     5461 aggttgttat gattgacaaa tattataatt gggcaacgct tggaaccggc gttattgcca
     5521 acgaattagc ccaagcactg gaagcacgtg gacaaaaatt atattctgta gctaatagaa
     5581 cttacgacaa aggacttgaa tttgctaaca aatatggtat ccaaaaagtt tatgatcaca
     5641 tagatcaagt atttgaagac cctaaagtgg atatcattta tatctctact ccccacaata
     5701 ctcacatctc atttttacga aaggctttag caaatggtaa gcacgttctt tgcgaaaaat
     5761 ctattacttt aaacagcact gaacttaaag aagccttaga tttagccgaa actaaccatg
     5821 ttgtcttagc tgaagccatg actatttttc atatgccaat ttaccgccaa ttaaaaacat
     5881 tagttgatag tggaaaatta ggaccgttaa aaatgattca aatgaatttc ggaagttata
     5941 aagaatatga tatgactaac cgttttttca gtcgtgacct agcaggaggt gctttgctgg
     6001 acattggtgt ttatgcactt tcttgtattc gctggtttat gtcagaagca cctcacaaca
     6061 ttacctctca agttacattt gcaccaacag gggttgatga acaagttggt atcctactaa
     6121 ccaatccagc aaatgagatg gcgactgtca gccttagttt acatgcaaaa caacctaaac
     6181 gagcaactat cgcttacgat aaaggctaca ttgaactttt tgaatatccg cgaggacaaa
     6241 aggcagttat tacttatact aaggatgggc atcaagatat tatcgaagct ggcaaaactg
     6301 aaaatgctct ccaatatgag gtagctgata tggaagaagc cgtttcagga aaaactaacc
     6361 acatgtactt aaactatacc aaagacgtta tggatatcat gacacagcta cgtcaagaat
     6421 ggggatttac ctacccagaa gaagaaaaat gaagagagct tgacaatgat tgtcaagctc
     6481 tttttagtta atatacacta cattttctta aaaaataggc atttaaagaa ataaatcata
     6541 ccaactgcta aactcaaaat agcataaaca tagaaaactg catttatttg tcctagtaaa
     6601 tgatcaaaac aagacagaag aatagccgca ctagctccac caatattaca aaatactaaa
     6661 actaatgtcg ttgcagaacc aaccaacgaa tattcaacac ggtctgctac taggctaaac
     6721 acagcagtga ccatcaggct gtaaaggaaa cctgatgcca tcgcactaat tgttagtacc
     6781 attagattag aaggcaatcc aactccaagc aatgttaaag aaaataagac gaggcagatt
     6841 ggaattaatt gcttatgaaa catagctatc aactgaccaa aactcatacc tgctattatt
     6901 cccataagca tcatcgcact aagaacaaga ctagcttgag ctggcgtacc caaaccaaat
     6961 tctactacta gcagaggtat cctaagatta atcccagtat ttatagttat gacaaatcct
     7021 gctaaaatag ctaaggcgac aatataagtc aaatcagctt tagggacttt ttgaccttta
     7081 gttttaatct tatcatttgt acctttcacc actttcttag gcgcaaaaac aagatataga
     7141 actaagattg gtaaagctaa aaaataaatc ataaatgatt tggaccaacc aaaagtcaac
     7201 aatagcccaa ctaaagctgt caaaccagca tttcctaaaa cttcaaaaga cccccttaat
     7261 cctaagagtt ttcttctttc tttgccttga taatattcac taatcacact aatggcccta
     7321 gcattaataa aaccaatacc actccccaat aaaagacgcg atacaaatac taaaaggtag
     7381 ttctgactga caaccggcaa tcctccccct aatgctgtta gcaatagccc aaagataatc
     7441 atatgtttct cagaaagttt tttacttaac caaggtgtga taagcaagat gaagataata
     7501 gccatcgaag gaattgaaaa taataattca acttggggtg atggtaagcc cttatctcta
     7561 taataagata tcatttgtgg tagggctggt gatgtcgaaa aagttgatag caagataagt
     7621 gataaagaga gtaaacttag cttctccata aagattttca agtgacaata cctctttctg
     7681 atgtcttttt ttatataaat aaagtataat caatattaaa ctagaaatat atgacatatg
     7741 tcctaaaact atctcaaata gggagtcaat acatggatat agaaaactac tgccaagact
     7801 actgtttgga gactatcttt ccaaaacatt atttcaagga tttacaagta atcaccctaa
     7861 ctgctggcca acccgtctgt aaacagggag aacaattaga atacttgcac tacatcgtca
     7921 agggacgttt taaaattgta cgtcgactct ttaatggcaa agaacatatt ttagatatta
     7981 aaacaaaacc aacactgatt ggtgatattg aacttttgac taatagacaa attgtatcat
     8041 ctgttattgc cttagaggat cttacagtca ttcaattatc acttaaagga cggaaagaga
     8101 aactgttaac agacgcaact ttcctcctaa aacttagtca agagctagct caggcttttc
     8161 atgatcaaaa catcaaagcg tctactaatc tagggtatac cgtcaaagag ttacttgcaa
     8221 gtcatatttt agcaattgaa gaacaaggtt actttcacct tgagttaagt agtttagctg
     8281 attcgtttgg cgtcagttat cgtcatttat taagagttat ccacgatatg gtcaaagaag
     8341 gccttatcca aaaagaaaaa ccaaaatact ttatcaaaaa tcgtttcgca ctagaatcat
     8401 taaatattca gccatgaagc gtagctttat catttaatta ttttatgcta aactaaatca
     8461 gagaatacag ttaggagaaa atatgtctgc tattgataaa ttagtaaaag ctagtcattt
     8521 aattgacatg aatgacatta tccgcgaagg caatccaacc cttcgcaaag tggctgaaga
     8581 agtcacattc ccactttctg agaaagaaga aattctcgga gaaaaaatga tgcaattttt
     8641 aaaacactct caagatccaa ttatggctga gaagctaggc ttacgtggtg gtgttggttt
     8701 agccgcacca caattagaca tctcaaaacg catcattgcc gtccttgttc caaacgttga
     8761 ggatgctcaa ggaaatccac ctaaagaagc ttatagcctc caagaagtaa tgtacaaccc
     8821 taaagttgta tcgcattctg ttcaagatgc tgctttatct gacggggagg gatgcctatc
     8881 agtagatcgt gaagtacctg gctatgtcgt ccgccacgcg cgtgtaacta ttgaatattt
     8941 cgacaaaact ggtgaaaaac atcgactcaa attaaaaggt tacaactcta ttgttgttca
     9001 acatgaaatt gaccacattg atggtattat gttttacgac cgtatcaatg agaaaaatcc
     9061 tttcgctgtc aaagaaggct tacttatttt agaataagga caaataacct agagataatt
     9121 ttctctaggt tttatttttt aatagcaaat aaaataagaa atcttttttt aaaaagtcga
     9181 ctctttccaa aacatctggc aatctacaat ctgttggtta tcgacggagt catctccttc
     9241 aatacgatta atcaaaatct gtgctacttg ttcaccttct tcataagccg gctggacaat
     9301 tgttgacact ttaggagatg aaaagtctgt ccattcaata ttgtcaaacc caactaaacc
     9361 aactcgtggc atatcaaaat tgagattttt catggcagta aaaaccattg gtaaagccca
     9421 acaatttggt gcaaatacta atgtctcctc cttatctgga actaccgctt ttagaaagtc
     9481 ttctatatca ctttttgaat gatcatcatc ttcaatcacc aaagtgtcat aaccgaaacc
     9541 attgtccttt aaagcatcca tgaatcccga agctctctca atacgtgtac taagcaagct
     9601 cgtatcagca gtaatcataa taaacttttt ataccctcga tttagacact cctgtgtcat
     9661 atcataaaca gcatcatagt tattagcctt aacccaactc gtcttatgtt cataaagttg
     9721 actatcaaaa aataccattg gtttcttttt ttcttttaaa atacgagaat actttcgaaa
     9781 atttgatgta ggctgaataa tgaaaccatc aacacctaaa tttaacatat tttcaatata
     9841 atcttcttca cttttggcat cataattact attacccaca ataatttgat agcctttttg
     9901 tttggtgatg tgctcaattc cttttacaat ttgatttgaa aatgtgtttg ttatatctcc
     9961 aattaagaca cctagtagtt ttgttttttt agaatttaga cttcttgcta tcgtacttgg
    10021 tctataacct gtttcatcaa tcacctcttg aatacgctgc ctcgtctcat ccgacatttt
    10081 ttcaaatttt tgattcaaga aaaaagaaac cgttgttttg gaagtttttg ataattgagc
    10141 gatgtcatta atggtcattt ttttagacat aacaaacctc ctttctaatt ttactgattt
    10201 tattttttta tggttaatat agtactagta tagcataata atagccctct taatttttta
    10261 aacgactcat tttgccatta tttttatcat atacaatgac ttttcctttg cattttatcc
    10321 cttgcatcaa atagagttta tccccaaaga taatatcatc catatgcact ccaattaaat
    10381 aatgaaagtc ttgagaccta atatcaaatg caatgctatt acttagttcc ttatgactgt
    10441 tcgtttgcac taaaggaaca catttcacct gacagttccg atcaactatc aaggtataat
    10501 ccatcacctc atcaacaaat tttattttct taactaattt atgactttct gtcaattgat
    10561 tataccgctt agaaataagg caatcttcta gattaaaggt cgtaggactt actaatttca
    10621 agtcgttaac aagcccattt tcataaatga cctgatcatc taaaatatat tgatttgtta
    10681 aaacatgctc tcctggacaa ttcaccttat caataactaa ggtaatggcc tgcggtaaag
    10741 ttaacaacca tcgcttatgg taatagatat cagaagctct ttggacatgt agttgtcctt
    10801 cgactaaatg gtacctatcc tttgaagtca gctgacaata gtcacatttt ggataagaat
    10861 tgtatgtcca agagtccttg atcatttcta aagattcccc atcaagggta catgttgaat
    10921 gactagtcga acgtttaaaa tcatacctta gttgttcttc tttgtaagta tatcttcctg
    10981 catcaatgaa aataggtttc tttttatcat agagacaaac actattatta tcactatgag
    11041 tatgagcgct accaaatgga ccgttcttaa aaaatatata acgcctatca tcacgtaaac
    11101 acacatgacc agaatctgga aaaagataag cagactcacc tattgttgca cgcggtattt
    11161 cttcaaaaag ataaattgat ggcttcccaa acaaaagtaa agtttctaaa ttaacattat
    11221 caaaagaaaa tgattttgtt tcagtaacag aaaaaaacaa cttgattgat aaatataaag
    11281 aattattatc aaaaatccca gagaatacac aaaatcctgg ggaaaagaat atacgaaatt
    11341 atctagagaa agaatatgaa gaactactac aaaaagatag attattcaag catgagtaca
    11401 cagagtttac aaaaagtcta aatttgaatg aaacattcta ttcacaatta aaagaaggag
    11461 aaatgaaact aagtgaaaat cccgaaaaag gagaaacgaa cacgaattaa cctttcaaat
    11521 atctacatca aataacagaa gtctccgtat caagcgaatt gtagcttttt tgaatatact
    11581 aaaaacactc ctacctctat tttactagaa gtaggagtgt ttttttcatt ttagttcata
    11641 gatgcagtgc cattgctatc cttgcctttt tttgtacagt attatccgat gactttaaag
    11701 cggtcatcat cattcatgaa ttctttttct cctgtttcag cttcgattga accaaaattt
    11761 agttgtccac gaagtttcca actagctgga acaccataaa cagcttgtac tgatgcatca
    11821 ataactggat tgtaatgttg taaattacca ccaagtccaa gttctgcaga aagggctgtc
    11881 caagtattta ctgaggcaat acctgttgat tgctcagacc aaactggaaa gttatccgca
    11941 taaagaacga attgttcttg aagagatttg acaacatctt gatcttcaaa gaagagtact
    12001 gttccctttg aagctccaaa actagctaat ttttccttag ttccagcaat agctgtctct
    12061 ggagcacctt gtactttcat tgtttctacc aaatcattag caaccaattc atcccagaac
    12121 ttagtaactt catcattcaa taaaatgaca acacgggaag tttgactatt aaaagcagat
    12181 ggggattggc gaactgcctc tttaataatc tctactattt tctcatcaga aacttctgta
    12241 ttgcgaccaa gagcgtaaat agagcgacgg ttttttaatt cttccaaaaa tgacatattt
    12301 tttctccttg ttcttcaatc tcagatatct tactacattt gttctagtga catatgaatt
    12361 agattaaagt tatttattac tatgtagtaa ttatattacc actatatcgt aaagtcaaag
    12421 aatgtgactc acaaggtttt aaattttttt aaaaggttaa tcaagtcttc ttgctctgtt
    12481 tctgttaaga ctgaaaaagc atcttctaca cgtttaatat gctctggtag agcttttttt
    12541 atcacttctt ctccttcagt tgttaaactc accaaaaagg ctctcttatc attcggacaa
    12601 gaatggcgta atacccatcc cttcttttcc atatttttaa taacaacagt catattacct
    12661 gatgtagcaa gcatattttc aatcaattct ccaattttca ttgtgccttt agcatagaga
    12721 acatccaaaa ctgcaaactg agttggtgtt aattcaaatt ctttaaatga ttctgagact
    12781 tttccgtcta ttgttctaaa tgcttttcgc atgacaacca tagatttaac cgctgaattt
    12841 ttaaagtttc ccatttcatc tcccaaacgt attttttctt attttatcac taattagtta
    12901 ttttaagcaa gaatatcatc tttgccctat caaaagcagc tggcaaataa taaggttgag
    12961 taactactca accttattgc ttagaagaaa tcatcaaaaa gactcaactg attgtcttct
    13021 ggtaaatttc ccaaaatacc catctcatca aatttttcaa ctaaggtact tgagactcct
    13081 cctcgtttac gaagctctgt ttttgataaa aattcaccat cttcacgtgc tcgaactatc
    13141 tgttttgcaa cgttttctcc caagccttcc atagcaacaa atggagggat tagcgtatcc
    13201 tcttcaataa taaagtcagt cgcatggcta cgataaagat ctaatttccc aaatttaaag
    13261 ccacgctcaa gcatttcatt aaccaactct aatgtcgtaa aaagatcatt ttcgacattc
    13321 gtcgcctcat tacgctgacg cttctcagtg atatccttca tgcgcgcctt aacggcatca
    13381 agtccagcac tcattgtgcg taattcaaaa gctttggcgc gaatagaaaa ataagcacaa
    13441 taataaaata ttggataatg tactttaaag tacgccacac gtaaggccat taaaacatag
    13501 gctgctgcat gggctttagg gaacatatac ttgatttttc cacacgattc aatataccaa
    13561 tccgggacat tattatcacg cattgcttgg atataaccgt tacgctcatc ttctgaaatt
    13621 ttcaaccata accccttacg cacgcgctcc atgattgtaa aggccatctt tggttgtaga
    13681 ccagcgtgca ttaaataaac catgatgtca tcacgacaac caataacggt tgataaggta
    13741 gcaatacctt ctttgattaa gtcttgagca ttacctaacc aaacatccgt accatgtgat
    13801 agtcctgaca gttgcaacaa ttctgcaaat gtggttggat gggtttcgtt caccatacca
    13861 cgaacaaagt tcgtaccaaa ctcaggaata ccaagcatcc ctgtaggagt tccaatttgc
    13921 tcttctgtca ctcctaaaac ttccgtacca gaaaataact tcataacgtc tggatcatct
    13981 ggaaggatat ttgatggatc aattcctgat aaatcctgaa gtttacgaat catagtagga
    14041 tcatcatgtc ctagaatatc taacttcaag acattctcat caatatcatg gaaattgaag
    14101 tgagttgtct gccaagcagc tgtcatatca tctgctggat attgaaccgg agtaaaatca
    14161 taaacatcca tatagttagg aataacaacg ataccaccgg ggtgttgacc tgtagaacgt
    14221 ttgactcccg cagcaccagt cgctaaccgt tcgacctctg catcgttata aaatttattg
    14281 taatctcgtt cgtaaccttt gacaaaacca aaggcagttt tttcagctac ggtacctacc
    14341 gttcctgcac gaaaagcata ttcttctcca aagatatcac gaacatctaa gtgagcagaa
    14401 ggttgatcat ctccagagaa atttagatca atatcaggaa ccttatctcc atcaaatcct
    14461 aagaaagttt caaacggaat atcctgtcca tctttcttgt acaaagtacc acatttagga
    14521 caatttttat ttggtaaatc gtaacctgaa ccacaagaac catctgtgat aaattcagaa
    14581 tgctgacaat ttgggcaaac ataatgaggt ggcattggat taacttcagt aattcctatc
    14641 atggtcgcta caaaactcga cccaacagac ccacgtgatc ctactagata accacgttca
    14701 ttagaacgtt gcaccaacat ttgcgaagca agatagataa cggcaaatcc attaccaagt
    14761 atagatgcta actctttttc gatacgtaaa tcaataatat caggaagagg attaccatat
    14821 atttcgaatg ctttagcata agttaattca gcaactcgtt cttcagcacg atcaacaaac
    14881 ggggtataga gatccccttt tacaacttca acgtcttcaa atcgatctgc aaaagtattc
    14941 gtattggtta caacgatctc ataggctaaa tctttaccaa gaaaggcaaa ttcatctaac
    15001 atctcatttg ttgtacggaa atgtgctttt ggcaaaggcg ctggttgagc atcctcccca
    15061 cgaccgatag tcctattaat catggcacct tgtccaagac tacgaacaat aatctcacgg
    15121 taaatctcat cttcaggctc aatataatga acatttcccg tagctaaaac aggcttatct
    15181 aaacgccgac cgacctctat taggtctcta ataatttgtt gaatccccgc ctcatcttta
    15241 attaaatcac gaaccaccaa ggggcgataa atagcaggtg gcatgacttc aataaaatca
    15301 taatatttag ctagggtcac tgctgcatca attccatttg ataacaaggc atcaaaaact
    15361 tctccatcag aacaggcagt acctagcaag agaccttctc tgtgagcatc taaaactgat
    15421 ctaggaattc ttgccacccc ttcaaaatat ttaacatttg aaaggctaac taacttaaat
    15481 atatttttaa gtccaacctg attttggaca taaatggttg catgctttat gcgtgccttt
    15541 ttgtaagagt cttcagctac caatttagta ttgagctcca tcaaattagt cacatcacga
    15601 ttttcccgcg cctcttttaa gaaaataaag agtaagcgac ccgttgcttc agcatcgtag
    15661 ttggccatat gatgatgttc caaagcaact tggaaacgtt tggttagagg ccccaaacca
    15721 tgacgcttat attcaggata aagatttctt gcaaattcta aggtatcaat gaccggctgt
    15781 gtgatcaaag gcagattatg acgttcataa ttcgcattca taaaaccaac gtcaaaggtt
    15841 gcattatgcg caactaaaac cgtaccttga caaaaattct gaaattcctg aaggacttgt
    15901 aaaataggtt ttgaacctct gacatgatta tccgtaatac ctgtcaactc agttgtaaag
    15961 tcagaaagag gatggcctgg gtcaataaac tcatcaaatt gctcaataat attacccttg
    16021 aacatttttg atgctgcaat ctgaattagg tcattattag ctgcagaaag acctgtagtt
    16081 tccacatcaa atacaacata agttgcttcg tgcaaattca tatcaacttc gttgtaggaa
    16141 ataggcactt tatcttcaac aatatttgcc tctaatccaa aaattgcttt tatacctgct
    16201 ttttttgctc tgtggtagcc gtgcggaaaa ctttgaacat tggcatggtc agtaattgca
    16261 atagctgggt gtccccactt agctgcagta tcaatgaggc tctcgacagt tggtaaagca
    16321 tccatcgttg acatattggt atgagcatga aattccacac gtttttgatc tgctggcatt
    16381 aagtctttac gttcatgatg cacaatctct ttgatatctt gaacattcat tgtcaaagat
    16441 ttagtaaagt tattgttctc aatattccct ctgacccgca accacgaacc tttagaaatc
    16501 atatcgtact tcttcaattc ttcatcatct ttagcccact tttgcatagc aaaacttgag
    16561 gtataatcag tcatcttaaa attaatgata tgacgtccag tgcgggttgt ttttcgttca
    16621 acactaaaaa ccattccttc aaaaacgata cgattttctt ctgtggtcac ctcaatcatg
    16681 ggtgtaatct ctgctttttc aaagccagct tgacgttgtt tgatacgctc tttgaaatca
    16741 taattttgag ttggtgtcac ttcttctgat ggaggcgctg aatcttctag tgatttttgt
    16801 gcttccaagg cttgcatagc ctcttggtta gctttttcaa gcaattgctc gcggttagtt
    16861 tcaaatgatg attttaagtc ttgcgtcatt tgttcatcag aaaccatatc aatagcaagt
    16921 ttaccaaatc caaacaatga aaattgttgt tccaagcgcg gcaaatgatt ctgacgaaaa
    16981 tgattattat tgacaaattg aggtgctgaa ataatcatct gactgccatt gtaatgaact
    17041 tttaattgtg aaaaagaact cttaaaactt gcactattac acaagggttc acaaaaagct
    17101 tgttgataat aatcctgtag cagatcatca gaaaaatcaa tagtttctgc tcttatatca
    17161 aaagtagcct ttatatctgc tttttcaaaa ctattcacta aacgagtttg taattcacaa
    17221 tacacctcaa ttggcaataa ttctggaaat gaaaaatgaa attcccataa gcgtgataga
    17281 gaatgaacct ttacctcgat aatatcagca gacgaaaaaa cacttaaatt cttaatttca
    17341 agcggcattt caatctgatc cataagtttc ttgaacaatt ctgacatagt tttctcctga
    17401 atatcttact tccattttat cacatatctg taaatatttc gagataacaa aaggaggctg
    17461 ttaaaaaagg catttattag ccttcttaac aacactctct taactgagac tatttatcat
    17521 aagcatctag actgtaggtt tcaataattt ggtttaattt actagcataa gcagtatctg
    17581 tcgcataaag acctgttaga gctgcagtag catccttata agaagaagta tttgatttcc
    17641 aagcagatgc atacttttga ctagatacta actcagcata atcatatagt gaagcagaat
    17701 aatttggata ggcgcgaaaa ggagcttgga tttgagtcat attgcctttc ccatcatctt
    17761 ctaaagtagg catttggaca gatttacctt tatattctcc tttgatgcca aagaggttat
    17821 aattaggagc cttagacaaa tctgattgtc cactggatga ttccaaaata gcttgtgcta
    17881 ataagactga cgcatacaaa tcataggtct tagaaatttc aatagctgtt ggagaaatcg
    17941 tttgaataaa agtcgtcgtt tgatccgatg cactagtgac tgtcggttcg acattatcat
    18001 ttttataatt gtaaaaccaa accccaccca aaccaaaaac taatagtgtt gttgttaacc
    18061 tcaataccaa tttatctttt tttcgacttt tcatgatggc tcctttctaa aatatcattt
    18121 cattataaca aaaggcagtt gctttttacc aataagaacc atactaaatt tcactccata
    18181 taaaaaagtt agcacatcag actaactttt ttatcggctt atttttttgt tagaatctct
    18241 aaagtttcaa ttaagttatc agcgtgaacc tcaatcgtat caccacttgc tttaattttc
    18301 acttcaacga taccttcaga agctttcttc ccaacagtta ctcgaattgg aagtccaatt
    18361 aagtcactgt ctgagaattt agaaccaaca cgttcattac gatcgtctgt caggacttca
    18421 taacctttaa gcatcaaatc agcttcaatt ttctcagtaa gatcttgtga ctcttgatct
    18481 ttgacattga cagtaatcaa atgtacatca aatggggcta attcttctgg gaaattaatc
    18541 ccccaagcaa agcgataagc tccttttggt gtcttattca caaaaagtcg ggcatgttgt
    18601 tcaatgacag ctgagagaat acgactaaca ccaataccat aacaccccat aacaatagga
    18661 tttgagcggc cattttcatc aaggatgttt gctcccatac tatctgaata acgggtacct
    18721 agtttaaaga tatgtccaat ttcaatacca cgagaaaatt tcagagtacc tttaccgtct
    18781 ggagaaatct ccccttcttt tacttcacga atatcaacgt attcagctgt gaaatcacgt
    18841 tctggattaa ctccagtaaa atgataacca tcttggttag caccagacac agcattagca
    18901 aggtcttgaa ctttacgatc agcaataatt ttaacagact caggtaggtt aactggccca
    18961 agagaaccaa aacctgcacc aaaaatctct tttgcttgct cttcacttgc aggctctaag
    19021 aaatcagccg ctaaataatt ttttaacttc acatcattga cttgatcgtt accaactagt
    19081 aaagcaacca cgggctgttc atcagcaata aacaatagtg tcttgatagt ttgattttta
    19141 tcaattgata aaaatccagc tacctcatca attgatttgc agttaggagt ttctactttt
    19201 gttacttctt caaaggtagc agccttagta gatggtttgt actcattcgt tgccatctca
    19261 aggttagcag catagcttga ttcagtggag tatgcaatag tgtcctcacc agaaactagc
    19321 caagccgaga gttctgcctt aatttcttct aaaacatcct ctggaatatc atcaatagaa
    19381 ggaatagttt tatcaaggac taaccaacga tttaaatctg tacgattggg agttactgcc
    19441 ataaattctt ggctatcttt acctcccata gcacctccgt cacctataat gcctttaaag
    19501 tcaagtccag cacgtgtgaa gatggcttca tatgctttac ggtaatcttc gtaagtgaca
    19561 tcaaggtctt cataatcttt gtggaagctg tatccatctt tcatgataaa ttcacgtgtt
    19621 cgaagaagtc cattacgagg acgcttctca tcacgatatt tagactgaat ttgataaagg
    19681 tttagtggta attgcttata agattttact gcatcacgaa caagagttgt gaaagtctct
    19741 tcatgagttg gtcctaagat aaaatccgat tgatcacgat tctttaattt ataaaggtct
    19801 tcaccatatg tttcgtagcg cccagattca cgccataaat ctgctgtaag aagagctgga
    19861 gctaacattt caactgcacc aatcttctca aattcttgac gcataattgt tttaaatttt
    19921 tcaatagtac ggttagctag aggcaaataa gcatagattc cagccgaaac ttggcggaca
    19981 tatcctgcac gaaccataag agcatggctg ataacctgag catcacttgg catttcgcga
    20041 agtgttggaa taagcatttt actttgtttc attacaataa gatacgacta atcaaagttg
    20101 attctttttc aattgagaat tgcaattgaa aaattcgtat ttcctttcac gttttattat
    20161 cttttagaag aaagctcgca tgatatcgtt ccaggttaca gcaatcatta atactaacat
    20221 aactgctacc ccagcgagcg taatataagt ttctgtttct tgtttaagtg gtttgcgacg
    20281 aatggcttct aaaatattca tcactatctt acccccgtca agggctggta tagggatgag
    20341 attcataata cctaagttga tagagattaa tcccattaag ttgagaactg tgacgaaacc
    20401 atttttagca gcttggctac tagcctgata gagagctact ggacctccca atttattaat
    20461 actaaaatga gcaataagtt tctttaattc atttaaaatt ctaaagaaac tttcccatgc
    20521 tagtttaaat ccacctaaaa gcttgtcttt aaagcttgtc tttagagctg gcataatgcc
    20581 gatgatgtag gatttatcaa ctttctgtgg cttaacgttg atcgttttca ccacctcttt
    20641 gctcttaatt ttaagggcaa gtttttggtt tttttctaaa tgactagttg acttttctac
    20701 tgctgctgtc aactgttccc agttagaaac tttgtgcgac cctatttgta aaatacggtc
    20761 attatttttt aaacctgcac tagcagcagg accattttct gatacacgga cctgattggt
    20821 tgataaatct tgaacacctc cttgaataaa agcaagtgca ataaagacta caagacctaa
    20881 aataaaatta ttcatcggac ctgcaaaatt ggtaattaag cgcccccata cagaagcatt
    20941 ctgatactgc acatctaagg gagcaatacg aatttcagta ccatcctcct caatgatagt
    21001 agcatcgtga tcaacactat aggtttttgt ctcacttaag accaatcctg tgatagttag
    21061 tttatcttct aaatcataag ctgttacatt gattggtaaa gaagtattgt ctaattgttt
    21121 tcctgataaa ttaatacgag ttacaatgcc ttctttattt aaagtaaggc tagccggcgt
    21181 ccctgtctta atttcagtct tatcatcacc ccaaccagcc atacggacat aaccacctag
    21241 cggtaatata cgaatagtat aggttgttcc ttctttatca atgtgagaaa agatttttgg
    21301 acccatacca atagcaaatt cacgaacaag aattcctgat ttctttgcaa aatagaaatg
    21361 cccaaattcg tgaacaacta caatgacacc aaaaataata ataaaggtta aaattcctag
    21421 cattcaattc ctctctaaat acaatatatt attttagaat aagccaaaga agtgcatgat
    21481 tgggaaaaca aaaatcatac tatcaaagcg atccaaaatc cccccatgac ccggaatcaa
    21541 tttacccgaa tctttaacac caaaatgacg tttaatacta ctttcaacta aatctccaaa
    21601 ttgaccaaaa atgctaaaga tagccactaa aactaacatg actaggaagc tatgaggcgc
    21661 atagacggtc ttatcaaaaa gcataaaaaa gaaagctacc acaatggctg aagcaattcc
    21721 tcccagactc ccttcaattg ttttatttgg agacactgaa ggtagtagtt tgcgttgtcc
    21781 aaattgacga ccaatcatat aagcaccgat atcagtcgcc cagacaataa ataaagctaa
    21841 caacacctta tctattccag ccatacgagc actgactaga ttttgaaagc caatacccac
    21901 ataaaaacta gacgcaattg gaaaggcagc atcttcaaaa ctgtaagaat tactgttcaa
    21961 aacagtacct gccaaaatca taaaaataac aataccgtat gctgataaac tagcgtcaac
    22021 tggtaaaaag gacaaataac tatctaaagg aactgttaaa acaaaggctc ctatcatagc
    22081 taatgcacct tcaaaggaaa atatttctaa acgacgcata cgaagtaatt cagaaacacc
    22141 aattatagct aaaagtccaa ctaaaaattg gaaaggcaaa ccacccatta ctaaaaaagg
    22201 gataaatatg gcaagcgcga cagctcccca aataacacgt tctttcattt tctctcttct
    22261 ttctatacac taccaaatcg acgatggcgt tgattataat ccacaatggc cttatgtagt
    22321 tcgtcttttt taaaatctgg ccataaaacg ggagtaaagt aaaattcact ataagctgat
    22381 tgccaaggta aaaagttact caagcgtaac tctccactag tgcggataat caaatcaggg
    22441 tccctataaa gatagggtaa gctatttgtc attaaatgat ttgctactaa atcctctgta
    22501 atatcgtcag gattcaactt agcttccaaa acatcctgag caatttcctt aatggcatta
    22561 gtgatttctg aacgtccgcc ataatttagc gcaaaattca atacgagccc cgaattatgt
    22621 tttgtcctca aacaagcgcg ttgcattgca tcgtatgttg ccttaggtaa tttatgagta
    22681 tctccgatga cctgtacacg tacattattt ttatctaact ctggtacata tttatcaaaa
    22741 aattcaaccg gaaggttcat aataaactta acttcatcat ctggacgtga ccaattttct
    22801 gtagaaaagg catagacagt tagtacttta acacctaatt ctgaagctgc tattgtaact
    22861 tcttgaagag catccatccc cgccttatgt cccataacgc gtggttttaa acgttttttt
    22921 gcccaacgtc cgttaccatc catgataata ccaatatgtg ctggtatttt ctccaaaggc
    22981 atttctactt gtttttttct tttaaatata aacataatgt tccttaattt agcaatcgtt
    23041 tttcttatca gccctttaaa agagctggaa atatcccata ctataacatt ttaccacatt
    23101 ttaacttatt tttgagtatt aaaatgccat ttgtgacagc aaactaaaaa gaccagaaca
    23161 atcatctggt cctacatatc ttattcagtt tcgatggctg tttctttatt ttcgtcttca
    23221 ttgacttcaa taatcttaac atcttcaact ggttctgttg gtgttgctgc cttagagaca
    23281 acagatttga tagctgccaa ctcaaacgtc aagtagacac catcaacatc taaaaccatc
    23341 cgttgtgctt cagtattaac ttcatcaact actccaaaaa gaccaccaat tgtgacaatt
    23401 tcatcacctt tttgaacagc attcaattgt ttttgacgtt cttgtgcttg tttcttttgc
    23461 tgacgttgca tgaagaacat cataccaacc ataacaacaa gcataataat aggtaagctc
    23521 atttgattct cctttccgtg ttctacgtaa tcattactat agcagattta tgctatatct
    23581 gcaaggagat actgtctctt agccaataaa ttcacggatt tcttcttttg tcatttttga
    23641 atcgcacttc accttgatag ttccgttttt aacaaccatc aaacctggca ctgtaggaat
    23701 gtcatattta tttcgaaaat ctttatttgc agtccaatga tcaaaatctt cactatctaa
    23761 atggaataat tcaaattcat cttcatcagc tacttctaca attttaggaa caaagaggtg
    23821 acaaaatgga caagaaatac gaccaatgaa cagtacaaaa tcttgatttg aatcaatttt
    23881 atcttcaatt tcggcagccg ttatcaaagt catggttgcc aaaagttctt caaaagaata
    23941 catagttaac caactttcta aatcttatat ttaaagaata acctatgatg gcaaaacttg
    24001 caaggcaaga gtaacaacga ttctatcaaa atcattaact cacttctcgc ttcaaactat
    24061 cctcaaaaat gctcactcat atttaaatga ctcatcaaaa aaactgagca tagctcagtt
    24121 tttattttac accttatttt tgtacagcat tactaacgct tttagcaact acatcggcca
    24181 catcattttg aaaagcattt ggaatgatgt gagttgtgct taattcttcc tccggtatta
    24241 aactagctat gcctctcgct gccgcaattt gcatttcaac agttatcgtt tttgctctag
    24301 catctaatgc tcctctgaaa atacccggaa atgctaaaac attattaatt tgatttggaa
    24361 aatcactacg accagttcca actatgtaag ctccggcttt taaggcttga tctggataaa
    24421 tttctggaat aggattcgcc attgcaaata caataggttt atcagccatc ttggaaatcc
    24481 attctgcatg taatgcttcc ggagcagaaa cacctataaa aacatcggca ttctccaaag
    24541 catcttctaa ggatccagat tggaattctc tattagtcaa ttttgcaatc gctttgtgat
    24601 gaggggctaa tgactcccga tctttatcgt taataatgcc aaatcgatca actaccgtta
    24661 catgttttgc tcctgctgat agaagcttcc ttgttataga taagcccgct gaacctccac
    24721 cattaacaac tactttgata tcttcaatgt cttttttaac taacttcaaa ctgttaaaca
    24781 aagccgctaa tactacgatt gctgtaccat gttggtcatc atggaaaact ggtatatcac
    24841 attcttcaat taggcgttgt tcaatttcga aacaacgtgg tgctgaaata tcctctaaat
    24901 taatccctcc aaaagtcggc gaaatcgctt ttactataga aataatttcc tctgtatcat
    24961 ttgttttcaa cacaattggc acagcatcta catttgcaaa acgtttaaag agtgctgctt
    25021 taccttccat gactggcatt gctgcctcag gaccaatatc tcctaatcca agaacagctg
    25081 aaccatcact tactacggct actgtatttt ttttagtcgt caactcatat gcaagctcca
    25141 catcctctgc aatagccgat gatactgacg ccacccctgg ggtataggca atactaagat
    25201 ccctcttatc ttccacctta acttttgaaa ttacttctaa ttttccacca ttttctctag
    25261 cttggttgat agctaattga cctaaatttt cactcatttt gataactcct tttgataaat
    25321 attagaataa catgcgtaaa atggctgtca tcgtgataac agtaatagct cctcctaaac
    25381 gcgtagcaac ttgagcaaat ggcatcaagt tcattcggtc tgctgtactt aaaatggcaa
    25441 catctcctgt tcctcccata ccactttgac aagctgaaat aatacctgct tcaacaggat
    25501 tcatattaag gaatcgcgat acaaagaatc caacagaaat aactgtaaat acaacactaa
    25561 taacaactat gaaatattgt atgctaagcg ttgccacaac atcttttaac gggatataaa
    25621 gcaatcctag ccctgccatt agaggaaatg taaaattacc agaaataaat ttataaagtt
    25681 gcttagctcc attttgtgtt tcactaggaa taacattgat atatttcaaa atagctgctg
    25741 cgataatcat taatacggga ccaggaaatc cagttaattg ctgcaacaaa tgtccaaaaa
    25801 taaagagact acatgctgtt aaaacacctc cacccatttt cttaacgtca attgggccag
    25861 aatgatcagc aataatatct gacatgtcct ctcctttatt taaccttact aattgccctt
    25921 gaccagacaa gtgtggtttc ttttctccca aacgattcaa tagtgcagta cataaaatgg
    25981 caaagaaatt accaataata gtggctggga tgagttgagc aactagttgt tcgctagcta
    26041 caccggtaat tgaactatag cctaacgata atggtaaaat accttcacca ataccaccag
    26101 ctaaaacggg tgttacgata taaaacaaag tgtgtcgcca ctctaagcct aaaataacac
    26161 cgacaaatgt ccctaccatc atcgcacaaa ccattcctaa taacatgggg aaaatcattc
    26221 ttagtaatcc ctgaatcaac attttccgat tcatccctaa aatactaccg gacactaaac
    26281 aagcaatata aaaataaaga aagttagctt gcttcatcaa catatttgtt gactctaaaa
    26341 catttttatt aatgaggttg aaaaacacca aaatagatgg tactaataaa gataaaatag
    26401 ctgagccacc gaagttttta aaaccaggga tgctagctcc aatagtacct aagaaccatc
    26461 ccattgttaa gatgactgca aatcctccta gcatattgac tggcaatttt tgtaaaaagc
    26521 ctgctagaag aatcaataat gctaaacaaa catatactgg taagggaact gagcctacct
    26581 taatatgaaa tagtttagtc tttaagtctt gcttttgacc tctagaaccc tcattattca
    26641 ctaccttcac gtctgccatg aaagcacctc cttttacttc ttatgtacag tttactttgg
    26701 gacttagtga taaggaagtt tatttaagta attgaattat ttcaacttat ttaattaatt
    26761 taagctggat tctagttttt aggctatgtt atactaaact cggaggatgc tgatgaaaaa
    26821 aaaactatct ctttgggcat ttctatcatt gattttagtc actatgacca tttgtatatt
    26881 ttctattttt tattatgtca ctatacatca gtcctatcgt atggtaaggg tacaggaaga
    26941 aaaaatctta aaaaatactg gttatgcttt atcgagaaat ccacaagtta tacaaacact
    27001 caaagataat cactataatc aaagtctcca aaaacaaatg ctattcctta gcaaaaaatc
    27061 aaatttagac tatattgtcc ttataaattt aaaagggatc cgttttaccc accctgactc
    27121 cactaagatt ggtaaaccat ttcaaggcgg tgatgaacaa gctgttttta agggaaaagc
    27181 tattatgtct actgcagaag gttctttagg aaaatcttta cgatacttaa ttccagtata
    27241 tgatcaccaa aaacaggttg gggcaattgc agttggctta aaattaacga cacttggaga
    27301 cttatctcaa agcagcataa aagagtttag taagcctctc ttgattagta ttttgattag
    27361 cttagtcgtc acaagtataa tttcttatgg cttaaaaaaa caacttcaca acctccatcc
    27421 aagtgatatt tttcaacatt tagaagagcg caatgcaaca ctggaccaaa ttcaagccgc
    27481 tgtatttgtt attgaccaga ggcatatcat caaaagaaat aatcctgcag ctagtttatt
    27541 attcaaaaaa gaggggcaac gagacctatt ttcaggtaaa ttattagaat ctttaattcc
    27601 tcaactcaag cagaatcatt tttcaaaaaa aactgaacaa gtattacatt ttcaaggaca
    27661 agactattta ctatctatct cgccaattac ggttaaaaca caaaatagag gctatgttgt
    27721 cttcttaagg aatgttactg aaacactttt cactttggat caattagcac acactacagc
    27781 ttatgcttct gctctacagg cccaaacaca tcaatttatg aatcaacttc atgttattta
    27841 tggacttgca gacatcgaat actacgacga gttgaaaatt tatttaaaag aattattgga
    27901 gccacaaaat gaatttttag cacgcttatc aatgttagtt agagagcctc gactggctag
    27961 tttcattatt ggcgaaagag aaaaattcgc agaaaaacac atcaatctat ccacagaaat
    28021 tttagtagaa atccctacca agtcaactgt tgaggatgtt aataactatc tcttgctcca
    28081 cagatatatt aatacgaaga tactcacgct attaaattca acgactttag tttctttacg
    28141 tttaaattat caaaataacc tcattgaaac agattatcaa tgggagaatg agaagtggtt
    28201 actcaatgac taccatcagt atttcaacga cgcttatttt caacagttac ttgtagattc
    28261 aagagctact tataagatct ctttaaaaca aaataaggtt attataagaa caaatattat
    28321 ctactcagaa gggtcttagt atggacgtat taattatcga agatgatcct atggtggaat
    28381 ttatccaccg caattacctt gaaaaactca attattttca aaacatctat tcaactgcta
    28441 gtcaaacaca agctattgct tatcttaatg acattaaaat ccaacttgtt cttttagata
    28501 ttcatattaa agaaggcaat ggtctagaat tattaaagtt attacgcaat caacaccaaa
    28561 ataccgaagt cattgttatt tcagcagcca acgaggcgca cactgtcaaa gaagcatttc
    28621 atttaggtat tgtagattac ctgataaaac catttacatt tgaacgcttt gagtctagta
    28681 ttgaaaaatt cctaaatcat tatcatacat ttgaagctga taaaatctat caagacaaca
    28741 tcgatcactt tcaaaaaata gattctggat ggctagaggg agaggttaaa cttgatgaga
    28801 aagggctctc tgaaattacc tatcagcata ttcttgatgc cattcaagag ttagagcagc
    28861 ctttcactat ccaagagtta gcaaaatgca gtcaattttc tcatgtctct gttaggaaat
    28921 atattgccta tatggaagaa aaaggccttc tgacaagtca gcaaatttat actaaggtcg
    28981 gtcgtcctta caaagtttat aagcttataa aaaaaactta ggcctgtaga cctaagtttt
    29041 tttaatcttc gtagccattt ggatgactag aatgccatgc ccatgccgat gaaataattt
    29101 tatcaatatt atcaaattta ggctcccacc caagcatttg acgagctttc tctgaggaag
    29161 caataagcgt atctggatct cctggacgac gagctgcctt ttgagcagga atttctttcc
    29221 cagtaatacg acgagccgcc tctaacatct gaaggttaga aaaacctgtc gaagatccga
    29281 gattaaacac gtttgattca ttcccttggc gaaggtaatc aactgctaat atatgagcat
    29341 ctgccaaatc aaatggatgc acataatctc gaacattagt tccatctgga gtattgtaat
    29401 catctccaaa aatcattatt ttgtcacgta ctccttgagc aacttgaaga atgattggta
    29461 acaaatgtgt ttctggttta tgatcttccc caattgagcc atccggttta tctccagcaa
    29521 cgttgaagta acgtagagcg acaaacttaa taccgtaggc ttgatcagcc catttcataa
    29581 ttgtttccat cataagctta ctttcgccat aaggattaat aggattttga ggagctgttt
    29641 ctaaaatagg aacttgttct ggaataccat atgttgcagc tgttgatgaa aagacaatat
    29701 ttttgatatc acattcattc attacttcta aaagttttat cattccagca gtgttattat
    29761 caaaatattt taaaggattt tccattgact cagcaactag tgaaaatgcc gcaaaatgga
    29821 taacagcatc tacatcagga ttttccctaa aaacttgacg cataaacgtt ttatcggata
    29881 aatctccttc ataaaaaatc gcatctgaat gcaccgcttg acgatgacct gttaccaagt
    29941 tatcaacaac aataactttc tcttttcctt gtgtgattaa ctgatcaacc atatgcgagc
    30001 caatgtaacc ggcgccacct aaaatcagaa ctgccatatt tttctcctat ttcaatactc
    30061 ttttaacatc atttgttacc tcattatact gagaataagc tttttttgca aatatctacc
    30121 gaaattagaa tagctctatg cagaaggcat cccacggctt gagagtgtta cttgatagta
    30181 cttcttgaac ctctgtattt gaaataatag tttctttaac agcttctgga aattcaaaag
    30241 attggttttg atcggataag tttgcaacta ttaaataacg ttctttatct gtttgacgaa
    30301 cataagcaaa taccttatct gctgtttcca aaagtttaaa atcagcatca actaaccagt
    30361 cgtgttcttt acgtaaagca actaattttt gataagtata aaaaatactt tccgtatcat
    30421 ttaacgctgc ctccacatta atttcctgat aattgggatt gactgctagc catggggtac
    30481 cgttagtaaa accagcttga tgtccttgac tccactgcat tggagtacga gcattatcac
    30541 gaccgacttt acgaatacta tctaaaattg ttgcttcaga taccccatta tccatggctt
    30601 ctttagcata gttaagggat tcaatatcat ctacatctgc taaacactca aatggataat
    30661 tggtcatacc aatttcttca ccttgataga tgtaaggcgt tccacgcatc aaatgtagta
    30721 ggattgctag agccttggca gattgttttc tgttatcact atcatttccc caaatcgaga
    30781 gcacccgagg aaggtcgtga ttattccaga ataaagaatt ccaaccttgg ccaagctcca
    30841 attgtgtttg ccattttgta aaaatatctt tcaatgctgg aacatctaat ccgtcactgt
    30901 aatcccactt aggagcgtca ggtttatgtt gaagacctac atgttcaaat tgaaagacca
    30961 tagataattc ttcattatcg ggatttgagt attgttttgc aatgtctggc gttgctcccc
    31021 aagtttcacc caccgtcaat aagtcatgct ttccaaaact agctctgttc atttctttaa
    31081 gataatcatg gagttttggt ccattaccag taatgccctt atctggaatt ttcccaatta
    31141 aatcaatgac atccatacgg aaaccaccaa tacccttatc aatccagaaa ttcatcatat
    31201 cataaatttt atgacgtaaa gcttcattct cccagttgag atctggctga cgtttactaa
    31261 aaaggtgcaa ataatattga cctgatgctt tatcatattc ccaagctgag ccactaaaaa
    31321 tagaagtcaa atcgttaggt tcatctcgcc aaatataaaa atcacgttca ggactattag
    31381 ggttttctcg cgcttctacg aaccaagcat gttcatcaga ggtatgattc actaccaaat
    31441 ccatgatgat tttaatcccg cgttgatttg cctcttgtaa cagttggtcc atatcattca
    31501 tgtcaccgaa aatatctgca atggcttgat aatctgaaat atcataacca ttgtcatcca
    31561 ttggactttg gtaaacaggt gataaccaga tagcagtaat ccctaatttt tcaaggtagt
    31621 ctagtttgct agtaatccct ttaatgtccc ccacaccatc accatctgaa tccataaatg
    31681 aacgtgggta aatttgataa atcgtagctt tatgccacca gtgtttcttc attattcttc
    31741 tcctatcaat aattcattca tcttttattt taacgttttt cctcttttag tgcaatcgtt
    31801 tgcgcaaata acattataaa aaggctggaa cccaaccttt ttatgaatat cgcttatacc
    31861 ttaacgaata gcttgttcag tatctgcatc aaagaaatgc cccttagcaa cattaaacgt
    31921 taggttaact ttttctcctg gattatggaa gtcacgcgct tcaacacgag aagcaaattc
    31981 tgtttgacct aacttaacat aaagcatagt ttcgctaccg agtaattcag aaactaaaac
    32041 ttctgcttca acattggcat ttggataagt atcttggaca agaagattgc ttgaaatgtc
    32101 ttctggacga ataccaaaaa tcaattcttt tcctttatag cctcgtgatt ctaaaagttt
    32161 ttcttgacct tcagggatag cgattcgaag accatcttca ctaataatca taccattttc
    32221 aactttaact ttgaagaagt tcatagatgg actaccgatg aatcccgcaa caaatttatt
    32281 agcaggaaga ttatagagtt cttgtggtga accaacttgc tcaattttac caattgtacc
    32341 atcaccatca gggttcttag tagctgacat aatgacaata cggtctgcca aagtcatcgc
    32401 ctctgtttgg tcgtgggtaa cataaatagt tgttgaacca atacgttgat gaatttttgc
    32461 aatttcagca cgcattgaaa cacgtaattt agcatctaag ttagataaag gctcatccat
    32521 taagaacact ttggcatcac gcacaatagc acgtcccata gcaacacgtt ggcgttgtcc
    32581 acctgacaaa tccgcaggtt tgcgctctaa gaattcagta aggccaatat tagctgctgc
    32641 ttcacgaacg cgcttatcaa tttcttgttt tgagaattta cgcaatttga gcccaaaagc
    32701 catattatcg taaacagtca tatgtggata aagagcatag ttttggaaaa ccatagcaat
    32761 atcacgatct ttaggtgatt tgtcattgac aacttcgcca tcaatcttga gctcaccttc
    32821 actaatatct tcaagacctg cgatcatacg aagtgttgtt gatttcccac aacctgatgg
    32881 tccaacaaaa acaataaatt ctttgtcttt aatatccaag tcaaagtctt caacagaata
    32941 atgcgatgca cttggatatt ttttgtaaat atgatttaaa tttaattcta ccattagatt
    33001 gacctctttt attattgtga ttttattata gcaaagaaag ggttttcata taatggtaac
    33061 cttgcctaaa tttcttgtgc aaactaacta aattttaggt atccatcgaa attaaatagg
    33121 cataggctaa atcatctaat atttttaaat taagccccga agattgggta aatttatcta
    33181 atttatattg caatgaattc cgatgaatga aaagtgcctt agccgtttta gcaaggttcc
    33241 cttgctcttg ccacaaggct tttattatag ccgaagtatc actcgtatca aggatatgtt
    33301 gtaaacattt tgctttaata gaaggcatgg gcgattgatt tgccaaagcc cataaagcca
    33361 tctttggaaa tcgacagtaa tgttgttcgg ccttgtgact aagaaaaagc gtaaaaagtt
    33421 tatattcttc ttcatataat tctttccagt ccactgcttg tagatgcgtc caactatttc
    33481 caaaatagca tcttaaacgc gtgttaaagt cattttcaat cgttggtaaa atatccgtta
    33541 gtaattccgt agcattaaag aaattatcct gatttaaaat catgacactt tgggtttgat
    33601 taattgtcat aattttttct acgtttatta ctaaaggcga caagaaatcc ttaagttctt
    33661 ctgataaggt aaattgatgc tcaataaaaa taaattggac tgccgaatga gattggggca
    33721 accttccacg accttcaact agatagcggt accaaggatt ctgagaatcc actgaactcg
    33781 catcttccag gcttggtgtc atttgtaata agcatagttc acgcttagtt agcgaagatt
    33841 ttggaaagtg gaggacatga ccatcttgct caacgatgat ataatcctca ctctgtttaa
    33901 cttcctgttt agtaataact tctggaaaca gttccttaaa atccatggca tcctccacta
    33961 cagttttgtt cattatatca tatttataac ctctttttga gcacagaaaa agaggagaat
    34021 gtctcctcag aaaatttctg ttataaaata gtgatccgaa tagaatgctg tttcgactgc
    34081 tttggattaa gccgtacgat attttgttta tcttctaaaa tatcagactc ttcaatagaa
    34141 gttgacaaag aactccaggg ttccaaagcc aaaaaaggac caccattgtt acttgtccaa
    34201 agaataagat tctcaaaatc atcaaagtca agttggattc cttttgcatg atttctagat
    34261 tttaagtaaa ccgtcttaga tcgtagttgg tctaaagtaa tagcatcttt ttcaaataaa
    34321 gcatgattaa gagataattg tttttgattt tctaaaaaag gatgtctagc ttgtaaatct
    34381 aacaagcctg tatccggaaa cgactcagga attgtgcaag tttcaaactg ttcaaactct
    34441 agatagtaat cactaaatac ttccttttca aacaaagggc aattaaagcc tgggtgagct
    34501 ccgatagcat atggcatatt tttttctgat tctaagtttt tgacaatata tgtaatggca
    34561 atgcttttat ttcttaaggt gtaagttatt tctaaactaa aacgataagg ataatttatc
    34621 aacatttctt tactactttt aattgtaaaa cgaacagaag aatccgatat gtaatcatag
    34681 tcgaatgttt tgtgacgtac aaagccatgc cttggtatga tacccgtaaa atgtggagct
    34741 tgagctggtc tgtaaatgac acaatcattt cgaacacttc cgcatatagg aaataaaata
    34801 ggagcttgcc caccccaata agtggggtcc ccttgccaaa ggtactcaac gccttgacta
    34861 tctgtaatag aagtcattgt tgcacctaac gcttttaatg tcacttgcaa ctcatgattt
    34921 tgtatcgtaa tagccattat ttttcctttc ataaagagca ataattgctt taccagcttt
    34981 tctctagcaa ttatctttcc ttccaagaag ttgctgtttt ttgcaaaatt ttatttagtt
    35041 catcaatatt ttgaaaacct attgtacgta accattgacg tgcaccagct tctccttttt
    35101 caacatactc tttaacactt ccagcccaag ttgctctacc acaaaggaca ccgttaaatt
    35161 tagcaccagc ttctttagca aacacaagag tctcttgaaa aagctgagca ggaacgcctg
    35221 cactcaaaaa aatgtagggg agtagtgtcg cctgatcttg ttctctaaaa taagccgcag
    35281 ctgtcgcttt attataagca gtctctcctt gcgcaaaacc ttctacatag tccatattga
    35341 caggcacttc caccttcaag acatcaatat taaatcgagg atttgaaaaa actttcatag
    35401 cttcaataac cttacgtggc ttaattttgg catactcaat acctgagcta tccgtaatct
    35461 tttcatcata tgacaaaatt tcaaggaaaa aaggaatgtc ttctgcaaca cattcagaac
    35521 caatccgttc aatataagct tctttttctt cgtttacttc atccgaacta tcaacatcat
    35581 aataaagtaa gaacttaact gcgtctgctc cctcttcttt aatgcgtttc gcagaccaaa
    35641 tgtctaaaca gtctgggagt cgcttggtac tactggtatc ataacccgtt ttttcgtacg
    35701 caagtaaaag accggcatta tcatctaaaa ctttcgtagc tggcaaccca tattctggat
    35761 ctaacaacat tgatgaagcg taaggggtta attcttcagc taccaaaact ttaagtgctt
    35821 ctatttgaga aacagtaggc tcctcagatt gatattttgc cattaatcgc ttcaatgctc
    35881 cacgttggtc aaaagccaag gccgaaataa taccattttt atcactcaat tgttccatat
    35941 gtttttgttt ttgttctgtt aatcccatcc tatacctctt tcacttggat ttgactgatt
    36001 aattcttgat agtaggtcag attgatatgc cctgttaatg tttcttgtgc attaagcata
    36061 cccaagacat tagctctctt aagcaagttt gtatcatcta gttgatgttc caaagcagac
    36121 gcgattcctg caaccgttgc atcccctgaa ccaactggat taataacttc tatttttgga
    36181 atcgtcactt gatagtagtg attcccatgt ttagcaaaag ctcccttagc acctaaagaa
    36241 acaataaccc aatcaattcc tctaaaaata tcttgctgta aggtagattt taattcatct
    36301 aaactatagc taattggctt tccaattaat tgtgttaatt cttcaaggtt aggtttaatg
    36361 acggtcggct tagcactaga ttttaaaacc gaacgtaatg cttctcctga acaatctaaa
    36421 acaactttct tattaaagtg attcgctagg gagattaacc tagcataata atctgagggg
    36481 agtccctttg gtaaacttcc tgatactgtt atgatagttg cagcatcaaa gataagtttt
    36541 agatgattca aaaaggcgtc tgcttcatct ttagtaatat gtggaccttt ttctagaatt
    36601 tcagtctgct gaccctcatg taaaactgca atacaatgac gtgtttctcc accaatactt
    36661 aaaaattggt gtctgatttc caatgcatct aagccacttc ggatgaaatc tccaaaatac
    36721 ccccctaata gaccagttgc cactacatta tcgccaagtt gagaaagaac acgagtcaca
    36781 tttaacccct tacccccagg tgttttggag acgtcggtaa ctcgatttac agtatccatg
    36841 ttaaagtttt ctaagcagta cgaaatatca atagaagggt tcaaagttac tgttaaaatc
    36901 atagatatct ccttagtcgt gatattcacc acgattccat ttatctaaga actctgtgaa
    36961 aaagtcattc tcttcttgat gggcattatg agtctcaacc tcggctatct tttcaattaa
    37021 tactttgttt tcttttgttg gtttatattt agctcttata aatgcctcga tgatatctgt
    37081 cattaacaaa ccaccagtaa ttttcccacc aaatccgata acgttagcat ttaattcttc
    37141 tttagcatag atagctgatg tcaaatcacg taccagagct gagcgaattc ctggaacttt
    37201 attaacagca ttattaatac caactccagt tccacaaata catacaccta agtcggcttt
    37261 tccactagcg acagcttctc ctactttctt accataaata ggatagtgag tacggatatt
    37321 atcataagtt ccacaatcaa taacctcata cccttttgtt tttaaataat ccacaactgc
    37381 aattttgtca taagttacaa tatgatcaca accgaccgca attttcatct gctttacctc
    37441 tcttacgctt agcacatttt attcaacata tcgacccgaa tttgatgacg acctgcatcg
    37501 taagtcccat caacaaaacc ctctactata tgtttagcaa ctcctggacc aacaatctct
    37561 gaacctaaag taatcatcct agcgttatta tgagctctag tcatataagc tgagcgttca
    37621 tctgaaactt ctgctgcaat catcccctta accttagtgg ctaccataaa aggaccaaca
    37681 ccgtaagcat caaccattat tcctaagtta tcttcttttt gattcacttt agcaacgatt
    37741 gctaaggtat tatcaacgaa atctttgcta gtatccgtta aatcaataat ttccttacct
    37801 aaacttgtaa ggtgttgtct aatcacttcc ttaagttcaa caccatgcgc atccgcccca
    37861 ataataattg tcatttcttt ctccttattt cacttttaat acaattgtat gtagtcggaa
    37921 gatattgttt gtttttgttt gtaatatcat catatatctt atttttgttt ttgtcaatgt
    37981 tttgttttgt tttttgtttg aaataaacaa aaaaactccg tgataactat gtcaactaga
    38041 attcactact agctagctat cacgaagttt gatacttctc attattttta tttgtaggaa
    38101 cggttattcc gcaagccata atcataaata aaatttaaac aaaacatgcc aatttgctgc
    38161 cattttctcc cataaagtgc cttgcttctc tgtattttga ctaagaaaac tccaattaaa
    38221 aactttgtaa tttaaagtaa aactattttg atgtttttct ctatgttcat aaaggacacc
    38281 aaattgatca ttattaagtt gttgaacaga gttgtaagca aacgaaccat cttgaatgtg
    38341 atggtgatat aaccacttaa aactaccatt tcgattaact tgcgctagac gtgcatatcc
    38401 atcttggcgc ttcttccctg gcccattagc attcactaat aaaatatact cttttttgtc
    38461 atgctcaaag cgaatagctg atagttggac gtaagcatca tgaatttcct tatatcgttt
    38521 aacatggttt tgccaagtct ccccgccgtc tttacttgtg gctacttcta ggttaccagt
    38581 tagattcctc ataaataact taatgtcccc atttttcaat tgaacgggta cggattctgt
    38641 attttgttct tttttattat ccattgttaa ggagtgaatt ttttcaccat tagaaagtat
    38701 acggttatca ttgactgctt ttccagtatg ccacgttttt ccatggtcgt ctgaataaat
    38761 taggcgtgaa gattgtgaac ctcttagatg agatttccaa ttcgtagaat aggcaggaat
    38821 aataatacga ccagcgtgtg gcccccattt taaaactata ccttttccag gccctattcc
    38881 caaaaatttc atgcctctct gacgaagact tgctgttata tctctaggtg atgaccatgt
    38941 cctaccatca tcatcgctat atgacatcca cacatagctt gattttgcta aacgaaatgg
    39001 tgacgtttta tgttttgtaa aatatatatt tccaagtagc tgttttccct tataaatatc
    39061 ccctagattt gaatggtcac ttttagtagt ttctgttata acatgataat cagtcttttt
    39121 ccctttaaaa ttatatacaa tacctttgtc acggatagta aaaaccttcg atttctttcc
    39181 attattatat aaattaagat aagactgtcc tccgatttgg gtatattctt tttcaggtgt
    39241 gttagcaata ctaataacgc ctcttccttc tggaaacata tcatatattg aaaaaatacg
    39301 tttcgtctta gagctagtat cttgaactaa tgccatatcc atattaatag gtgagccact
    39361 atagtcacca ctagtaacta gcggaactct agggttatta cggagattga caatagtttc
    39421 tctttttccc caagtgacac catcatcctc actacggcga ataaccattc ctatgtttcc
    39481 ccaatcacaa gcttgctcaa ttctttcatc agctcccgca agtaaagttc ctttttccgt
    39541 ttttaataat gaggggatac ggtaaccaaa cacattatct ttattttttc tcccaaaatc
    39601 tcctccccta tatacttctg ttttagcaga aagtatagca cctttaggta atttgagctg
    39661 atggtttaga tgacaataat attgaaaata agattttata tcttcaaaat tcaaattatc
    39721 aacacagcaa acaaggcagt gtgccttagc ttttaatttt tgccataaat taccactata
    39781 ttttatttcc ttctctactt tctctttgcc aatagcaagc atcccctcta taacagacga
    39841 taactttcca tccttttgag agaatgtttt agtaagatgg tgttcctgta tcgcattctt
    39901 tttaaagccc aacgatatct gactagcaat gtccgtatta cctatgacat tacttgtcga
    39961 taaatctccc ttaggaatga tatccaaaga cctcaactta tccctttcaa aacctctatc
    40021 ctctcccgct gtctttctaa attcagcgct attaagtaat gcaaccgaag aaccctttga
    40081 ttctatcagg aatctctgtt gttctgcaat ggaatgactg ctttcgggta taatataggt
    40141 agttctatta tcagcttgag ttacatttag ttctgctaaa tttgcctgtt tacgattttc
    40201 ttctgttgct atttccgctt ttagaggaga tgacaatttt tcttgactgt cagaatcacc
    40261 taagactatt accatatctt tatctttttg atcatctact attaaaccgt ccgatgtatc
    40321 agaatctaat tctaactacc tctcctctat tgaattagta agcttatcta aagtagagtc
    40381 aaatatggga gcagtagttt tttcatcatc tattgatata aaagagt
//
LOCUS       DATRSD010000007        38400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRSD010000007 DATRSD010000000
VERSION     DATRSD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.007"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..417)
                     /locus_tag="VBD85_000298"
     CDS             complement(<1..417)
                     /locus_tag="VBD85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7132874.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLDKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDL"
     gene            complement(407..889)
                     /locus_tag="VBD85_000299"
     CDS             complement(407..889)
                     /locus_tag="VBD85_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7132875.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            1106..4324
                     /gene="hylB"
                     /locus_tag="VBD85_000300"
     CDS             1106..4324
                     /gene="hylB"
                     /locus_tag="VBD85_000300"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO7132876.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKENTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGSATFDNISMKAKGPKDSEHPQPVTTQIEESVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDKFTKLLDKWNDVTIGNHVYDTNDSNMQKINQKLDETNAKNIKTIKLDSNHTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITATLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLD
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKMLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            complement(4376..5422)
                     /gene="rfbB"
                     /locus_tag="VBD85_000301"
     CDS             complement(4376..5422)
                     /gene="rfbB"
                     /locus_tag="VBD85_000301"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO7132877.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPYVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(5629..6222)
                     /locus_tag="VBD85_000302"
     CDS             complement(5629..6222)
                     /locus_tag="VBD85_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO7132878.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            complement(6222..7091)
                     /gene="rfbA"
                     /locus_tag="VBD85_000303"
     CDS             complement(6222..7091)
                     /gene="rfbA"
                     /locus_tag="VBD85_000303"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO7132879.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(7150..8253)
                     /locus_tag="VBD85_000304"
     CDS             complement(7150..8253)
                     /locus_tag="VBD85_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO7132880.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            complement(8262..9050)
                     /locus_tag="VBD85_000305"
     CDS             complement(8262..9050)
                     /locus_tag="VBD85_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO7132881.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            complement(9040..9723)
                     /locus_tag="VBD85_000306"
     CDS             complement(9040..9723)
                     /locus_tag="VBD85_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO7132882.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            complement(9827..10507)
                     /locus_tag="VBD85_000307"
     CDS             complement(9827..10507)
                     /locus_tag="VBD85_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO7132883.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTSDSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            complement(10625..11143)
                     /locus_tag="VBD85_000308"
     CDS             complement(10625..11143)
                     /locus_tag="VBD85_000308"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO7132884.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            complement(11266..13830)
                     /locus_tag="VBD85_000309"
     CDS             complement(11266..13830)
                     /locus_tag="VBD85_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO7132885.1"
                     /translation="MRPIVRKKMYKKGKFWVVAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDTSKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YEVKFTLKGQSVLTPTIPKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVPGTVGESKRLEALEVKLVAKASDFSSKTDHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNSSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNNIAGS
                     TNQGKRIEAIEIKLVETAKIPVTVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEATLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            complement(13982..16180)
                     /gene="recJ"
                     /locus_tag="VBD85_000310"
     CDS             complement(13982..16180)
                     /gene="recJ"
                     /locus_tag="VBD85_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO7132886.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALNMMGAEVQVYLPNRFTDGYGPNESVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFAGLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMTLGTPKEIYDFMMS"
     gene            complement(16177..16938)
                     /locus_tag="VBD85_000311"
     CDS             complement(16177..16938)
                     /locus_tag="VBD85_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7132887.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNTIEKIYGEFGQIDILINNAGFGSFKEFWDYSGEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFSNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFALTPEKVAKKLVSILGKN
                     KRELNLPFILAFAHKCYSLFPKTADYFARKVFNYK"
     gene            complement(16940..17869)
                     /locus_tag="VBD85_000312"
     CDS             complement(16940..17869)
                     /locus_tag="VBD85_000312"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO7132888.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTALRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            complement(17911..18558)
                     /locus_tag="VBD85_000313"
     CDS             complement(17911..18558)
                     /locus_tag="VBD85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO7132889.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYRDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGKYYYSSQGRESVIYIDTKSTN"
     gene            complement(18551..19789)
                     /gene="hflX"
                     /locus_tag="VBD85_000314"
     CDS             complement(18551..19789)
                     /gene="hflX"
                     /locus_tag="VBD85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO7132890.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSKDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            complement(19880..20770)
                     /gene="miaA"
                     /locus_tag="VBD85_000315"
     CDS             complement(19880..20770)
                     /gene="miaA"
                     /locus_tag="VBD85_000315"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO7132891.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLMAYREKLESLSDEDLFEKLTEQSIIIPQVNRRRAIR
                     ALELAKFGNNLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMMDNGLLDEAKWLYDN
                     YPSVQASKGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            20881..21057
                     /locus_tag="VBD85_000316"
     CDS             20881..21057
                     /locus_tag="VBD85_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO7132892.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            complement(21099..22016)
                     /locus_tag="VBD85_000317"
     CDS             complement(21099..22016)
                     /locus_tag="VBD85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO7132893.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYVRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(22151..25909)
                     /locus_tag="VBD85_000318"
     CDS             complement(22151..25909)
                     /locus_tag="VBD85_000318"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO7132894.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVTTGVCIFLHSPQVFAEEV
                     SVSPATTAIAESNINQVDNQQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     RIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTDAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSSNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVSLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQID
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVMTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDRSLDGKLKNQFGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGQPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEEIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKESKTVEKVNRIANKTSITPVVSKK
                     ADSYLTNEANLPKTGDKSSKILSVVGISILASLLALVGLSLKRNRHLK"
     gene            complement(26078..26812)
                     /locus_tag="VBD85_000319"
     CDS             complement(26078..26812)
                     /locus_tag="VBD85_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132895.1"
                     /translation="MRDNHLHTYFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLAHFDYGFRKLNLDVEDLKP
                     FETQLKRIFIKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     LEHFCYEFDRLQGLLKDYQIDENHLI"
     gene            complement(26827..27411)
                     /locus_tag="VBD85_000320"
     CDS             complement(26827..27411)
                     /locus_tag="VBD85_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO7132896.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEARLYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKAYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            complement(27508..28914)
                     /gene="pepV"
                     /locus_tag="VBD85_000321"
     CDS             complement(27508..28914)
                     /gene="pepV"
                     /locus_tag="VBD85_000321"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO7132897.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFASKHNLKAELSVEDEQYTATVYGKSAHGSTPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGPLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            complement(28961..29563)
                     /locus_tag="VBD85_000322"
     CDS             complement(28961..29563)
                     /locus_tag="VBD85_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7132898.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            29733..31514
                     /locus_tag="VBD85_000323"
     CDS             29733..31514
                     /locus_tag="VBD85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO7132899.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKHQWR
                     LLSKIILTSLFISVIGTVALAVVAGTSSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVQHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRGDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGRFASHIPSFAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADKFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDELN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            complement(31556..33337)
                     /gene="uvrC"
                     /locus_tag="VBD85_000324"
     CDS             complement(31556..33337)
                     /gene="uvrC"
                     /locus_tag="VBD85_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO7132900.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     EIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            33496..34263
                     /locus_tag="VBD85_000325"
     CDS             33496..34263
                     /locus_tag="VBD85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO7132901.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(34322..35662)
                     /locus_tag="VBD85_000326"
     CDS             complement(34322..35662)
                     /locus_tag="VBD85_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7132902.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERIQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(35762..36178)
                     /locus_tag="VBD85_000327"
     CDS             complement(35762..36178)
                     /locus_tag="VBD85_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7132903.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPEIEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(36182..36679)
                     /locus_tag="VBD85_000328"
     CDS             complement(36182..36679)
                     /locus_tag="VBD85_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7132904.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            36901..37497
                     /locus_tag="VBD85_000329"
     CDS             36901..37497
                     /locus_tag="VBD85_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO7132905.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            37686..38400
                     /locus_tag="VBD85_000330"
                     /pseudo
     CDS             37686..38400
                     /locus_tag="VBD85_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        12320 a         7562 c         5930 g        12588 t
ORIGIN      
        1 caaatcctgt gctaaaaaat taggattaca atcaattttt gttaattctt ttattggaag
       61 ccatacaaaa tgtcgcttat gcgttccttc tttgatagat tctggcgcct gacccaagag
      121 attaacaaaa tataaaaatt caactgagtg ataatgatgt ctcttaatat taaactgatt
      181 ctcaacaata taggctaaat caccaacttg gctgtgaaga cctatttcct caagtacttc
      241 tctcgcaaca gccttattgc tatcttctcc aacttgaagc gccccacctg gcaaatagta
      301 tttatcgaga tgaggagcat atattaatag cagtttttga ttttctatga ttaaagcaga
      361 aactctgact ccgaatgttt cgagctggtc tctaacccta aaatctctat ttttcataaa
      421 aattaaccgt cttttcaatt aaattttggt gttcatcata aacaaactta gcagaaaaga
      481 aaggaacatc ttctaaaatc catttaaaaa tttcgtaatc tccttgccac gttggcttag
      541 agagaacttg atcatatggt acccattcta aggttccttc tcgtgattca tcatctgaaa
      601 ttaactctcc ttcataatct gttactttaa agacataggt ataccaatca tgacctggcg
      661 taaattcagg aaaagtgatg actcctttaa aatccatttt ttttactgtt aaatgggttt
      721 cttctagaat ttcacgctta gcacattcat ccggagtttc acctgcctcc agtttaccgc
      781 ctaccgagat ccactttccc tcatgaacat cattctcttt tttgttgcgg tgcaatagaa
      841 gtaattcctt gccattgtca atgtaacaaa ttgttgctaa tttagtcatt tcaaactcct
      901 ctcattctca tattataaca aaaaatagcc tttatttagg caatttacta tcaaagtagc
      961 acacaaatga ttaaacaaat tattttgttt accagttatt tacttttcac tttgatgaca
     1021 ttttaaaggt tctttatcat aaacctttaa agcatgctat aattaggaaa aaccggttta
     1081 caattttaaa ccctaaggag taaaaatgga aatcaaaaag aaacatcgta ttatgcttta
     1141 ttcagccctt attttaggaa caatattggt taacaatagt taccaagcta aagctgaaga
     1201 gcttaccaaa actacctcaa cgtcccaaat aagagatact caaactaata atattgaagt
     1261 tctccagact gaaagtacca ctgtcaaaga gactagcacc acaaccacac aacaagatct
     1321 gtctaacccc acagcttcaa ccgcaactgc aacagccact catagcacaa tgaaacaagt
     1381 agtagataat caaactcaaa ataaggagct ggtgaaaaac ggagatttta atcaaactaa
     1441 ccctgtatct ggaagctggt cacatacaag cgctagggaa tggtctgctt ggattgataa
     1501 agaaaatact gctgataaat cacctattat ccaacgtacc gaacaaggcc aagtaagcct
     1561 atccagcgac aaaggcttta gaggtgctgt aacacaaaaa gtgaacattg atcccactaa
     1621 aaaatatgag gtcaagtttg atattgaaac aagtaacaag gctggacaag ctttccttcg
     1681 tattatggag aaaaaagaca acaatacgcg actttggctt tctgagatga ccagcggtac
     1741 tactaacaaa cataccttaa caaagatata taacccaaag ttaaatgtct ccgaggtgac
     1801 acttgaactt tattatgaaa aaggaactgg ttctgctact tttgataata tatcaatgaa
     1861 agcaaaaggc cctaaagact cagagcatcc acaacccgtc acaacacaaa ttgaagaaag
     1921 cgttaatacg gctttaaaca aaaattacgt ttttaataaa gctgactacc aatacactct
     1981 aaccaatccg tctctcggga aaattgttgg tggaatattg tatccaaacg ctactggttc
     2041 aacaactgtt aaaatatctg ataaatctgg taaaataatt aaagaagtac cgttatcagt
     2101 tacagcttca acagaagata agtttacaaa actcctcgac aaatggaacg acgtgactat
     2161 tggtaatcat gtttacgata ctaatgattc gaacatgcaa aagattaatc agaaattaga
     2221 tgaaactaac gccaaaaaca tcaaaactat caaactggat tctaatcaca ctttcctttg
     2281 gaaagattta gataatctca ataattcagc acagttaacc gctacttatc gtcgtttgga
     2341 agatttagct aaacaaatca ccaatcccca ctctactatt tacaaaaatg aaaaagctat
     2401 tcgtactgta aaagagagtc tggcttggct tcatcaaaac ttctacaatg ttaataaaga
     2461 tatagaaggc tctgccaatt ggtgggattt tgaaatcggt gtccctcgct caattacagc
     2521 taccctagct ctcatgaata actacttcac tgacgctgaa ataaaaactt ataccgaccc
     2581 aattgaacac tttgttcctg atgcaggata tttccgtaaa acgcttgaca atccatttaa
     2641 agcccttggt ggtaatctag tcgatatggg gcgcgttaaa atcattgaag gtttacttcg
     2701 taaagacaat actattatcg aaaaaacttc tcattctcta aaaaatcttt ttactactgc
     2761 tactaaagct gaaggtttct atgctgacgg ttcttacatc gaccatacaa atgttgctta
     2821 tactggcgcc tatggtaatg ttctgataga tggtttgaca caattgctgc ctatcattca
     2881 agaaactgac tataaaatct ctaatcaaga acttgatatg gtttataaat ggattaatca
     2941 atcattttta cctttaattg taaaaggtga gttaatggat atgagtcgtg gacgctcaat
     3001 tagtagagag gcagcttctt cgcatgcggc tgcagttgaa gttctcagag gtttcctcag
     3061 attggctaac atgtctaatg aagagcgaaa cttagacctc aaatcaacta ttaaaacgat
     3121 tatcacttca aataaattct acaatgtctt caataacctc aaatcgtatt ccgatattgc
     3181 caacatgaat aagatgctta atgacagtac agtcgctact aaacctttaa aaagtaattt
     3241 atcaaccttt aatagcatgg accgcttagc ttattataat gccgagaaag actttggttt
     3301 cgcgctttca ttacattcta aacgtaccct caactatgaa ggaatgaatg atgaaaatac
     3361 acgtggttgg tataccggag atggtatgtt ctatctttat aatagtgatc aatctcatta
     3421 tagtaatcat ttttggccaa ccgtcaatcc ttataaaatg gctggaacaa ctgaaaaaga
     3481 tgctaagcgt gaagatacca ctaaggaatt catgagcaaa catagcaaag acgctaaaga
     3541 aaaaaccggt caagttacag gaacatctga ctttgttggt tccgtcaaac ttaatgatca
     3601 ctttgctctt gccgctatgg attttactaa ctgggatcgc accttaacag cacaaaaagg
     3661 ttgggttatc ttaaatgata agattgtctt tttaggtagc aacatcaaga atactaacgg
     3721 cattggaaat gtttctacaa caattgatca acgaaaagac gattctaaaa caccttatac
     3781 tacatacgtc aatggaaaaa ctattgattt aaaacaagca agttctcaac aatttacaga
     3841 tacaaaaagt gtctttttag aatcaaaaga acctggtcgc aatattggtt atatcttctt
     3901 taaaaatagc actattgata ttgaacgcaa agagcaaaca ggtacttgga acagcattaa
     3961 tcgtacttct aaaaatacct caatcgttag caatcctttt atcactataa gccaaaagca
     4021 tgacaacaaa ggtgatagct atggttacat gatggttcca aacattgatc gcacaagttt
     4081 tgataaatta gccaacagca aagaagtaga attactagaa aatagttcaa aacaacaagt
     4141 tatctatgat aaaaacagtc aaacttgggc tgttatcaaa cacgataatc aagagagtct
     4201 cattaacaat caattcaaaa tgaataaagc gggactttac ctagtacaaa aagttggtaa
     4261 tgactatcaa aatgtctatt accaacctca aaccatgaca aaaacagacc aattagctat
     4321 ctaactagcc gtacataaaa aacctatcat caaaatgata ggttttttgg ttcaattact
     4381 taatgacttc ttgagtttta gcatacttgg cttctacagc atctttttct gctttccacc
     4441 atgtctcatt ttctgtatac catttaatag tttcttccaa accttctgaa aagtttgtaa
     4501 attgtggttc ccagccgagt tcttcacgca attttgtaga atcgatagcg taacgaagat
     4561 catgaccagc acggtcagtt acgtgatcgt aagcgtcttt tggttgaccc attttctcaa
     4621 ggataagttc aagaacctcc ttgttgtttt tctcaccgtc ggcaccaata aggtatgttt
     4681 caccaatacg acccttagtc aaaatagccc agacacctgt tgaatggtca tttgtgtgga
     4741 tccagtcacg gacgttttta ccttcaccat aaagttttgg tttgattcct gccaagatat
     4801 ttgtaatttg acgtggaatg aatttttcga tgtgttggta tggtccatag ttatttgaac
     4861 agttcgaaat ggtcgctttt acgccgaagg aacgtaccca tgccttaacg ataaggtcag
     4921 aagctgcctt agttgatgag taaggtgatg atggattgta ttttgtttca gcagtgaatt
     4981 tttcacctgg tccttcacca tttcctggaa ggtcttcacg aagtggaaga tctccataaa
     5041 cttcatcagt tgacacgtgg tggaaacgga tatcgtattt acgagctgct tcaagcaaag
     5101 tgtaagttcc gataaagttt gtatggataa atggacttgg atcttccaat gagttatcgt
     5161 tgtgactctc agccgcatag tgaacaatag catccgcttt ggcagccaat ttgtctacca
     5221 attcagcgtc agcgatgtca ccaacaacta actcaacacg atcaccaaga atggcttcaa
     5281 tgttagcacg gttacctgca tatgtcaatt tatcaaggac agttacatga acatatgggt
     5341 ggttattgta aacatagtgt acaaagtttg atccgatgaa accagctcca ccagttacga
     5401 taatgttttt aaattcagtc attttttctc cgaaaaattt tttctttttg ctgtttgaag
     5461 gatactgttc tgttagaagt agttctatgg aatgtcatgc ctaagaaatc aatatttcaa
     5521 atccctgagg gaacaggcct tttgcatgtt tcctttcacc cttgcgtcaa acaacattgc
     5581 taattcgttt ttacttgcta aggttaatgc cttttaagaa gttgaaaatt acaaatcttc
     5641 cttcttcaaa ggttttacat ctttgagaag tgggtgattc ttatctgctt ctgagacttc
     5701 tgcttcttca aggttttccc actgaatgcc tagatttgga tctgcatagt taacgaaagc
     5761 atattttggt ttgagttcaa gtgcccaata atcgttaacg agataagtat aagctgcttt
     5821 atctgaaaga acttggaaac cattagcaac gccgcgtgga acaaagatac cttttgaggc
     5881 atcgataatc gtttggtaaa cgttaccaaa actgtcacct tcacggaggt caacccaagt
     5941 accgatcaca cgtccttcat cagcgatcga aacatattta tcccaaggct ctgcatggag
     6001 acctcgcaat gtattttttt tgttaaacga aatattattt tgtagtttgt ctgcctcaaa
     6061 gaagctttct gggaaaccaa gcggtatcat tttttctttt tgaaagttct ctttgaacca
     6121 tccacggttg tctccacgaa cggtaatatc gaattccaac aatccaggaa tggcttcaat
     6181 tgggcgacaa gttaattctt tatcaaaaaa ttgttcagtc attaggcttc tccaatcaaa
     6241 cgaagtaggt attgaccata ctcatttttc ttaagtggtt gtgccaactc aaggacttgt
     6301 tcacgagtga tgtagcccat gcggtaagca atttcctcaa ggttagcaac ttgaacgttt
     6361 tgcatgcgtt gtactgtttc aatatattgt gctgcttcaa gaagactttc gtgagttcca
     6421 gtgtctaacc acgcaaatcc acgtcccatt aactcaactg ataaatcacc tctatcaagg
     6481 taggctttgt taacatcagt aatttcaagt tctccacgag gacttggctt aatattttta
     6541 gctatttcaa ccacatcgtt atcatagaaa taaagacctg tgacagcata attagactta
     6601 ggttgcgctg gtttttcttc aatagaaatg gcattcatat cagtatcaaa ctcgacaaca
     6661 ccaaaacgtt ctggatcttt tacttgatag ccaaagacgg ttgctccact ttctttacta
     6721 gctgcacgtt gtagcattgc agataaacct ggtccatggt aaatattatc acctaatact
     6781 aaggcaacgt gatcatcgcc aataaattct tcaccgataa tgaaggcctg tgctaaccca
     6841 tctgggcttg gttgttcagc ataagaaaga gagattccta gttctgaacc atctccaagc
     6901 atatcttcaa agcgaggcaa atcttgtgga gtagaaataa tcagtatttc ttttattcct
     6961 gccaacatca aaactgacaa tggataataa atcattggtt tatcataaat tggcatcaat
     7021 tgttttgatg cagcacgagt taatgggtac aagcgagtac ctgaacctcc cgctaaaata
     7081 atacctttca tagtaaggtc tccttattta tattaatcct acatattata acactttttt
     7141 taaacgctat catctactaa tctttttcac gtatttcgta atatcgtaat cctctaaatt
     7201 tagctgattt tctttattta agattaattg acataattca taaccaatta atgggcccac
     7261 tgttaagcca gttgatccta atccacttgc agcatatgca ccctccatac aagggacagg
     7321 gccaaaaaat ggagaaaaat cacttgtata ggcacgaatt ccaacacgtt cagaagtcgt
     7381 atctgctttt tttaattgcg gaaaatagcc aatagcttgc tcttcaaact tgtctaagac
     7441 tttaaaatca atgtttaaat caaaagccat atcattttcg tgacttgcac ctacagagac
     7501 tttaccatta tcaaatggga taatatctag ctcgccttct ggcattacaa caggatattt
     7561 tcctgtatta atattgctaa aataatagtc tcgcaattgc cctttttgag ggcgaatatc
     7621 tacctgatag ttgtgctcat ctaatatctt agctagccag gctccactcg ctaaaacaag
     7681 tttatcaaaa tcaattcctt gaacacgaaa gccattatca gttatcgtaa agtgaacttc
     7741 atccttgatg acattaacac cacttgcttc taacaatatt cgtgttaagt ccgccccttc
     7801 tactcgcgct cctccactcg cataaagtaa ctgttcatag ccatctaact caggaaagtg
     7861 agtatttgct tcacttttat ttagtatttg taaatcacct attagaggag attctagtcg
     7921 acgtttatct gctagtgcaa acaatgactc taactgactc tcgtcctttt ttaataagaa
     7981 aacaccagtt tgctgataaa attttgtttc aaaaccatct ttttgtaaat ccgtgactag
     8041 ctttgaataa aaatcagccc ccaacctcgc catgcgatac caggctttat tacgacgttt
     8101 tgaaaaccat gggctaataa tccccgcagc agccttagtt gcttgtccaa ctccataatc
     8161 aaaaacagta acctgaatat ccttttcttt tgataagtaa taggctagtg tcgctccgac
     8221 tgctcctgca ccaataattg ctatcttttt cataatagct cctacatatg gtaaaaggga
     8281 tttgttggag ctttactctc taaaactgaa atatcccagc ctttttcaag tttccactgc
     8341 tcaatcatag tagctatttt agaaacaaat aaaacctcaa tatgatggcc tgggtcaatc
     8401 gcaagcaaac catttgtgat catttcttgt gcagtatgat aataaatatc tcccgttaca
     8461 aaaacatctg ctccttttgc aatagcttct ttgtagaaac tttgcccact acccccacaa
     8521 atagcaactc gttgaatttc aggattagat ttatcatatg aaactaaacg aacgctatct
     8581 aaaccaaaca catctttaat tttccatgca aaaaattcaa aactctgggg tctaacatca
     8641 ccaatgcgac caataccata accattactt gtctcagata atatatcggg atattgaata
     8701 tctaataatt cacaaaacca gtcgttaagt ccattaggaa cgatatctat attagtatgg
     8761 ctcacataaa cagcaatgtc tgatttaaga aggtctatat aaatcttatt ttgcggtgtt
     8821 gctaccaaat cctttaatgg acgaaaaata ggagcatgct ttacaataag aagatctact
     8881 tgacgttcaa ttgcttcagc tactgtagtc tctcgaacat ctaaagctac catgactgtc
     8941 tttatttctt tatctaaaga tccaatttga agacctgaaa tatctccttc catagataat
     9001 tcctgtggac aataagtttc atatacatca ataagttctc tagctttcat caagaacctc
     9061 ctttatatcc tgtattttat cttccaaaat agcacgttct tccattttac tattgggaat
     9121 agagtttaaa gcaaaagtta atttattaag ctcattttgc catttttctt taaaaactgt
     9181 tgtgttattg gataataaaa atggaccaaa tcgcaattct ttagcagtta aattcatatg
     9241 accatgctta acaactaaaa tttcatagca tttgtcattt tcagtcaaga tatcttctgc
     9301 tactatttca aaatcatttg cagctaacca ttttcttaaa tcatcttccc gattattagg
     9361 ttgtaagact agtgttttaa cgtgctgaag tttgtctata tcgttattca aaatatcagc
     9421 aatcaaacga ccacccattc cacagatagc tatcgtatca atattatctg cttcttcaaa
     9481 agctgacaaa ccatttgcta agcgaacatc tatctttgaa gtgagaccat gttctgaaac
     9541 gtttttcaac gcagactgat acggtccatt caccacttca ccagcaatgg caaaatcaca
     9601 gtatcccatc tgcaacaaaa aaataggtaa gtaagcatga tcacttccta catctaataa
     9661 ccgagctcct tttggaacgt agtttgcaac tttttgtaat cgttttgata attgtaaatc
     9721 cattgtattt ccttcagttt ttctctgtta aaagtataac atcattaaca tcaaaagaaa
     9781 aggagcagac ctaatgtatc aaccattctg tctcctccta gtaaacttat tcccataaat
     9841 tcattgcatt tttaaaatca tccgaaatcg ctaaatcttt catttggtta tcttcacgtg
     9901 cttgttttct ctcttcaatt tgtcgaagtg tcgtcaatcc ttcacgccgc caattgcgaa
     9961 ggatagcatt gatataattc caacttgttt tcccattgaa aaccgcttct ctaagagctg
    10021 ccctaacaat atcaggatct gtctgatctt cttgcagtgt cttttgtaaa tcttctaatt
    10081 caaaaggact taataagcga cctaattcac gttcgaagtc tcctactaaa tctttcagac
    10141 gattcgaatc agaggtttta ttgtctatta ttacttgatt atcctcttcg aaaagtttat
    10201 ctaatttagc aaacacaggg cttgtatcaa agataatctc gatttcatga ttcaactcaa
    10261 ttgttttgac atctaataaa ccttgacttg ttaaattcga tatagaacga ttgatatctg
    10321 ccactgtttt atcaagtgat gtcgcaatac ggctcggtgt tagatcggat aaattagttg
    10381 tattttgtaa atagaaaaat tgccaaacta aaaaatcgtc tgctgtctta aaaatagatt
    10441 taaagtgaaa aaataaagca cttggtagag tcaattgccc tgactgatat tgttccaaat
    10501 aagtcataac ctttcaaatc ctctctatcg tcctaaaatt atgaaatgtg taacaaaaag
    10561 cacctctcaa ctattatcat agcacttaga ggcgccctta tcattcatct atcttgaata
    10621 ggccttatcc tggaaagttc atcaatacct tagtatcata accttcaata gctttacgac
    10681 catttaatcc atctagttca actaggaaag cacagcctgc gacaacgcca ccgagttttt
    10741 caatcatttc aattgtcgct tttactgttc caccagtagc taaaaggtca tcaacaatta
    10801 aaacacgttg accaggttta atagcatctg cgtgcattgt caatgtatca agaccatatt
    10861 ctttttcata gtcagctgaa ataacttcgc gtggtaattt acctggttta cggactggag
    10921 caaaaccaat accaagagca taagcaactg ggcaaccaac aataaagcca cgagcttctg
    10981 gtccaacaat catatcaata tctttatcag cagcatattg tacaatttca cgaacagcat
    11041 agctgtatgc tttaccatca gccattaatg gggagatatc acggaaagta attccttctt
    11101 gaggatagtt ttcaattgaa gcgatgtaat tatttaaatc catttcactt gtctttctaa
    11161 ggggaaaccc ctaaaatgtt ttactcttca ttatatcata aaattaaata aaatagtact
    11221 ggaaacttta aaaccatatg ctagttttgc atatggtttt aaaaattagg catctgttac
    11281 cttgacaaaa ggtgctccaa tatcaattct atcctcacta taatagctct gttctttcca
    11341 aagttgtgaa attggatacc aaccagacaa ggacggtgtg taagggtcag agacatgtgt
    11401 cagtccgtta ttatatcctt tgagaacgat ttcatgactt ccatgaagaa caaaaacatt
    11461 attttgtact gccgctagga catgataacc atccttcaac gcttgtacaa ggttagcttg
    11521 agttcctaat gcagttgcag ttacacccca ttctttggct gcttttaaaa ttcctctaga
    11581 agttgttcct tgaacaccac gattaaattc aactgtctta tggtaaaggt aatctgcaat
    11641 ttgcgttggt aaaacagttt ctcctttcaa agaagacaaa atcatagcta gagaagttgg
    11701 tacacaacca gtttctccta aagttgccat accatatctt cgacttgccc accttccatc
    11761 acgttggcta aagtaaggag tattataatt tgaatgtgcg acttgtagag aagtttttac
    11821 caataattgt ttttgattag aagcattttc ctgataaaca tggataaagt atttaccatt
    11881 gtctctatga tttgtaacat taaatgtcaa tgtagcttca tgatttttgg gtgtgacggt
    11941 ataccactta aggtcatcct gattattttt atctgaccaa atagcaattt ttaaattccc
    12001 aggactaata acatttgtca ctcgtacatt atagtttcca gttcccttat aggtcgtttg
    12061 gacagttacc ggtattttag cagtctcaac taatttaatt tcgattgctt caattcgttt
    12121 tccttgatta gtactaccag cgatgttatt gttataagtc caatcctgcc aaccataatc
    12181 ttgcatgtga actcgataca tcacactata ggttttcgat agcagacctg tcaatcgcat
    12241 ctgaatagct tctacttgtt gagattcatt tggtacatta ctttctctcc ctgaattaac
    12301 aacagtttgc caacctgaac tattaagaaa agttctatat tctatatttc ctagagctgt
    12361 actacctaag ttaagtttga tggcttttat agattttgaa agtccaatac tacctcctaa
    12421 ttggccttcc aaaaccgttg gtttccaacc atctttttga agataagttt gataacttaa
    12481 tccaggatgc ttcatttcta agaatgaatg atctgttttg gaactaaaat ctgaagcttt
    12541 agctactagt tttacttcca aagcctctaa acgtttcgat tctccgactg tacctggtac
    12601 actattattt ttttgccaag ccatccaacc tttatcttgg acgtgagcgc gataataaat
    12661 atcataattt tgttttaaat tcccacttaa gtccaattga atagcttcta gtctttttcc
    12721 ttgataatcc ttacttgttt gactacctaa aatttctccc caacctttat cttgtacatg
    12781 agtacgatag agaatgtccc catacttcaa ggtgctaaga gtgaatttta ctccatctag
    12841 ggctttggat tctcctaaag ttcccgccat ttgtccttca agtttattag attgccaacc
    12901 attttggcct accttaacct gataatttaa gacaggtctc tcttccttag gtatagttgg
    12961 tgttaaaaca gattgtcctt taagggtaaa ttttacttcg taagcttcaa ggcgctttga
    13021 cattcccaaa gttccagcgt aagctccatt tttagcccaa gccatccacc ctatatcttg
    13081 aacatgaaca cgataataga catcatataa tttttgtaaa ttatcagtaa ggttaatgct
    13141 tagtgcttca ataggtctac tctgaccaac agtaccacta acttgaccac tctctactga
    13201 aggttgccaa ccttgtcctt taacataagt tttatagcta agttttcctg ttattccttc
    13261 aggtgcttta atagacaatt ttattgcctc aacctgcttt tcttgtccta ctgttcctga
    13321 aactgttgca ttatcaaccg acttttgcca tccaatatct tgaacatgag cagagtaagt
    13381 cactgttggt aattgctttg ttgaattttg ttgagtaata acttcactat cattagctac
    13441 ttgattaaca ttagaatttg tttgatttgc tgagtgctca gcagtactag cttttacaac
    13501 aacttttgag ctagcatcaa aggttttgac tggggtagct gctatctctc taggattaga
    13561 tgaaacagat gtagcaaagc ctgtttttat taaaggctct gcactatttt tcatagcctc
    13621 cgaggaatta acaggtaacg ttaacttgga agtatccgaa atagtctgac tgctatcagt
    13681 tttctctgag atcgttgaag ttactgcttc tgcctgttct gccttgacat cttgaccaag
    13741 aattgcacta ccacctaaaa tagtaacgat acctgcaacg acccaaaatt tacctttttt
    13801 gtacatcttt tttctaacga taggtctcat agattttatc ctcaccttta atattcttta
    13861 tcaaaaattc cattatcttc ttaattttat tacattttaa taatatttgc aatattatta
    13921 ttgaaataac ttatttatat gctaaaaaaa cctcttaaaa agtggcttct ttagtatatt
    13981 atcaactcat cataaaatca tatatttctt taggtgtccc taaagtcatt aattcttgaa
    14041 acttaactgt ctcttttaat tcttgataaa tttgactctc agagatatct cgcttttctg
    14101 cttctttatt gactgtcata atcccctctg taatcgtaac aaaatgtagt tcttcaaaaa
    14161 tttgaatcat tttaattaat aaaatatcag ggatatgaag gtaggaactg agctctttta
    14221 acttataacg aacgtcaaat tctgggaatt gatatattgt tttatataaa ccagcaaact
    14281 gatcacgtgt accataacca gatataaaat aattttttgg tatctcattt ttaaaataga
    14341 tggcatcaaa ctgtttacct tgagtcattt gttttaattc ttgtggatga tcgggaacag
    14401 tcaaaagaat aacttctttg ctctgctctt cttgagacag gattggcact ccatgaggta
    14461 agctacttgc tttgcttcta atatcaaata attgaatacc gtctacacgc gcatcctcca
    14521 acattaattg tagggttgtg gcaccattcc attgattaac agataaagtg acagctaact
    14581 ccaaatgttg tgcttgttgg aactcttgta gttgactccc catattaaat gctaccaaat
    14641 caagtgcttg tccatcttgt tctagtttta atttcagatg agcaccgttt tgccccatga
    14701 ctctagcttg actcacttta aaatctttta acaaaaagac tggcttttta ttatccattc
    14761 caaatggcgc taacttttca aaatctctaa cagtgtctaa actaagttcg tcaaaacgta
    14821 gctcactatc aattgtcaga gtttttttct gactcatgtc caattgtttt ttagagatat
    14881 agtcacataa aacttgagaa agatcaccca ccttactttc ctctaaagtc atgcctgctg
    14941 ctccgctgtg acctccaaaa gcaatgaata attctcgatg ctggtcaaaa gcttgaaaaa
    15001 tatcaagtgc ttctacactt ctggcacttc ctttagcaat accatcttca atattcaaaa
    15061 caataacagg ttgtccagtt ctctctaaaa tacgacccgc cacaatacct aaaacccctg
    15121 gatgccaatt ttcttttgct aaaacttgta caggctttgt ttgatcaagc atttgcatag
    15181 cttggtcaaa gatagtttga acgatttctt ttcgctcttc atttttttga tcaatcattt
    15241 gggctattgc ttgagattct tcatcatcaa accctgtcag taattcaata gctggattag
    15301 ggtcatcaag tcttcctaaa gcgtttaatt gtggagcaat tttaaatcca atagtttctt
    15361 catttagtgt ttttaaatca atatttgata gagatattag ctcctgtaag cctattcttt
    15421 cactatcttt cataacttca agtccagctt ttaccatgat acgattttca tcagttaggc
    15481 taaccatatc tgcaatcgtc ccaatagcaa ccaaatccaa catttctgtc gggattgttt
    15541 ctaagagagc acaagctact ttaaaagcaa caccacaccc tgctaaataa ggaaatggat
    15601 aattagcatc aggatgttct gggtgaataa tagcataagc acaaggtaaa tctgctggca
    15661 tactgtgatg atcagtaact accacgtcca caccttgatt ttgagcatat gcaattgctt
    15721 catgccctgc cacaccatta tcaaccgtaa tgattaatga aacatcttgt tgttcaataa
    15781 aatatttata aacactttca ttgggaccat aaccatctgt aaagcgattt gggagataaa
    15841 cctggacttc agctcccatc atatttaaag cttctttcat gattgatgct gatgtcattc
    15901 catcagcatc atagtctcca taaactaaaa tagtttcatt attttcaata gctcgtctaa
    15961 tacgattaac agctttatcc atatcattta acaaataagg atcatggaga ttttccaaat
    16021 tagtttgtaa aaaatgatgt aattccgctg atgtcttaat acctcgcgaa taaattaagg
    16081 aagccaccgc ttcacttatc ttttcttttt ttgaagcctc aaaaaagcca gcgtctggct
    16141 tttggttatt gaggacccaa ctatacttag cagatatcat ttataattga atacctttct
    16201 agcaaaataa tcagctgttt ttggaaataa actataacat ttatgcgcaa aagctaatat
    16261 aaaaggaaga tttaattctc tcttattttt tccaagaata gaaactagtt ttttagctac
    16321 tttttctggt gttagagcaa aacgacctat acttgctaaa taatcgccac ttggatcagc
    16381 ctgagcaaaa aatcctgttt tgattggtcc agggtttaca cttgtcacat aaacattttt
    16441 ttctgctaat tctaaacgaa gagcattcga aaatccaacg acagcaaact tactagcccc
    16501 ataaacgcta gctttacttg ttgctatgag gccagccatg cttgcaatat taacgatatg
    16561 accagatttt acaagagaca tcttatgtcc tatctgacga gctatagaca tcgttgcaaa
    16621 tgtattcaca gcaaacatgt ctttgacttc ttcaccacta taatcccaaa attctttaaa
    16681 agaaccaaat ccagcattat taattaaaat atcaatttga ccaaattcac cataaatttt
    16741 ttcaatcgta ttcgttacag cattgtcatt agttatgtct agtgataaac ataaggtatt
    16801 tggtctcttt ccataaagct tttcaagttt ttctcttgat ctccctgtga caattaaatg
    16861 gtcgtcttga ggtaattggt taataattgc ctgtgctaaa ccaccagaag cacctgtaat
    16921 cagtatcgtt cttgccattt tatataccta cttcttccaa atctctaaca agatgggtat
    16981 ttgaaaaaat cgtctttgca tcttcttcca tttgccaaca atctcgcccc ataaaacgag
    17041 cactaacatg atttaataat agtcgtttag catttgcttg tttagcgata tcagctgctt
    17101 gcatattggt tgaatgccca tgcgatttag caattcgctc atcaccctta ccataagtag
    17161 attcgtgaac taaaacatca gcgcccaaag ctaacctaat acttgcatct gtttttctag
    17221 tatctcccaa aatagtgatt actttaccct ttttcggttc agaaatataa tctttagcaa
    17281 taatttctct accatcttct aaggtaacat tctcaccatt tttgactttc ccaaaaagag
    17341 gaccaaatgg aacacctgct agtttcaatg cttcagcatc taaagtgccc tcaagatctt
    17401 tttgcacaac acgatatccc atacaaaaga tagtatgatc tagcttttct gcataaacgg
    17461 taaatttatc tgtttccata atttttccga gtgatgattc atcaaactcg tgaaaatgga
    17521 ttcgataagg taaacgtgaa cctgatgtcc ttaaggcagt cataacaaaa ctttttatac
    17581 caaccggtcc ataaatatct aaatctgtct gctcttcatt ggcttgaaaa gcccgactgg
    17641 ataaaaaacc tggaagacca aaaacatggt ctccgtgcat gtgcgtgata aaaatttttt
    17701 taactttacg aggtttaatc gtagtttcta aaatttgacg ctgtgtaccc tctccacaat
    17761 caaacatcca aacctcgtta atttcatcta ataatttcaa gactaaactt gaaacattac
    17821 gagctttcgc tggttgacca gctccagttc ctaaaaattg aatttccata cttgttgact
    17881 tttttataat ccttccataa cttaataaat ttaattagta gatttagtat caatataaat
    17941 aactgactca cgtccttgac tagaataata gtatttacct gactttgttt taaccaactc
    18001 taatagctct tcttgattga aactagttat tttaataata tcttttttaa gttgtaagac
    18061 attccctagt aaacttgttt ctggtgtaat attttgagat tctgtttcgt caaaatctat
    18121 ttttgccatt ctgataccag accgatctcg atagacttta tattgaggaa aaatctgtgc
    18181 aatttctaag agtaactgcg gtgtaatgac ttctttaact tcagaaaaaa cactcgcttc
    18241 ttcttgatca tttaggtaag caaaaccaaa tgattttcct gataaattca aacgaataaa
    18301 aggtttttct tcatcaaacg ataaatgttt tggaaacaag cccaaggctt tggaaagatc
    18361 aaaatagtaa tcagttgcag cttctggccc ttgcttttga tatatttcag caaaataagc
    18421 attaatcaca ctaggaggtg gggtgataaa atcaagtcgt tgttgcttag agaggccatc
    18481 tagttttttc tctagataaa ctttatctaa gctcgtaaaa ccaccataag tcaaagctaa
    18541 atcaatataa tcaatcataa aattcttcca gcttccattt ttgtttagga gaaatatagc
    18601 ctgtaatatt ctcgtattct tcttcaaagg tgtaggtatc aagcaatgct attttattga
    18661 gatcatagag cttatatgct tgattttgat gcactctaat cgaaaattcg tcaaagatat
    18721 gtcgtatttc atccacaata agacggcgta ataaatcctt actcccttgt tttttggctg
    18781 caattctcac attaggaaaa gttgttgcat tcagctgctc agtgacatcc atcttgttgt
    18841 atatcgctaa acgaggaata tctatcatat ctaagtcttt aagaatctcc ataacaactt
    18901 tttcgtgttc ttcatgatta gggtcactag catcaataac atgaagtaat aaatcgacat
    18961 gtcgactttc ttctaaggta gacttgaaag ccgcaactaa ctctgtaggt aaatcttgaa
    19021 taaaaccaac agtatctgtt aaagtgactt gaaattgatt ttgtaaatag atctgcttgg
    19081 ttgtagcgtc taacgttgca aataattcat tggcttcata ttgcttatca tcggttaaaa
    19141 cattcataat agttgacttg ccagcatttg tatatccaat taatccaatt ttaaaggttg
    19201 tcgaatcaac acgtctttct cgaactgtct cgcgattttt ttctacaatc ttcaattgtc
    19261 tttcgatatc agaaatttga tgacgaatgg aacgacgatt caactctaac tgagattctc
    19321 ctggacctcg gcttccgatt cctcctgctt gacgactaag cataatacct tgtccaacta
    19381 agcgtggtaa catatatttc aattgcgcta gatgaacctg taattttcct tcatgacttc
    19441 tagcacgcat agcaaagata tctaaaataa gttgcatacg gtcaataact ttaacaccaa
    19501 gttcagcttc caaattagag ttttgtcgcg gagtcaaacg attattaacc acaacggtat
    19561 caatctcatc tgcttctaca atagctttta tctcctctaa tttaccagat ccgataaatg
    19621 atttactatc atatttatct cttttttgat aataatgatt aacaacattt gctcctgctg
    19681 tcttagctaa gctagcaagt tcttccatgg acatttcaaa attctcagta tcttgaagtt
    19741 ctaccccaac taaaataaca cgttcttgct cttctttagt ttctatcacc tataatctcc
    19801 tttcaatatt tttttcttgt tctaaatacg taatctttat tataacataa tgccctttaa
    19861 atgcgagaaa cggctttatt tactttgatg ataaaaaatc actgactttt cgcttgattt
    19921 tttgttgata attttcctca ccaaccataa taaattcgac attcatacgg tttctaaacc
    19981 atgttaattg gcgttttgca aaacgtctgg tattttgttt tagtttatca acagcttctt
    20041 ctaatggtat ttgctttgaa aagtaaggaa ataattcttt atagccaatt cctttacttg
    20101 cttgaacact cgggtagtta tcgtatagcc atttagcttc atctaagaga ccattgtcca
    20161 tcatcaaatc aactcgtcta ttgatacgat cataaagcac ctgacgatca tcattcaatc
    20221 caattaataa aacatcatac ggactttcac tattttgcaa attattccca aacttcgcca
    20281 attctaatgc acgaatggca cgtcgacgat taacttgtgg tataataatg gattgttctg
    20341 tcaacttttc aaataaatct tcatcactta gagactctaa tttttcacga taggccataa
    20401 gagtttcttg attgacttcg cctcctaaat gatagccctc aaacaaactc tgtaaatata
    20461 ggccagtacc tccaacaata ataggaattt tccccttaga aataatcgta tcaatagcta
    20521 cttttgcctc tttgacgaaa tcataaaccg aatagttctc atttacttca cgaacatcaa
    20581 tcaagtgatg ataagcttgt tcttgctctt ctttactagc tttagccgtt ccgatatcta
    20641 acttctgata aacttgctga ctatcaccac taatgatttc gccattgaaa gttttagcta
    20701 attcaattcc taatgctgtc ttcccaaccg cagttggacc aacaactgct ataagcttga
    20761 tttttctcat gatacttctc aattctataa aggtttttac ttgaaattta actagtattt
    20821 cgttaccata attatatcat agatagaagc agagtcttca tcatagattg gagaattatt
    20881 atggttaaaa aattcgcatt tgctaaagga atagcaacag gagtcgttgc aacagctgct
    20941 actcttgcag gtgctgcatt tgctatcaaa aaaacaatta ttgaacctga ggaagaaaaa
    21001 atagctttta tcgaagaaaa tcgtaaaaaa gctgcacgta aacgtgtctc tcattaaaca
    21061 ataactccct tactagctag taagggagtt attttatatt aaattgttcg ccttaaaaag
    21121 aaaatataac gtaccattac tatcataaca ataagagttg ttgtcccaat ttcaaagtgg
    21181 actagcccct gtatatcaac tggtaatgat agatgctgaa tacttaattt aagtgaggta
    21241 gctgagataa caagggggaa agtaaaggct gagaaacctg gtgtaaaatc accaatcagt
    21301 aatttaggca cctgaaaaag aatgaaaaaa taaagaaatt gtgccattac gattaatagc
    21361 agtaataaac ctctatttgg tgacacaaaa ctattgacat atcccgctgt gattagcgac
    21421 attggagcgc aaatagtagt tatattaggc ttaactgccg aaggtagccc gattttatat
    21481 gctttaatga atagaaatgg taataatact aaagttgcaa taaaaccata ccaaaagact
    21541 attttcccaa tagtgaattg tccactaatt ggtgctgtca ggctagctac ccctatacca
    21601 acatagagga cagaccaaga aggaaaaaga ttagccatcg aaaagtgtct caaatatttc
    21661 catgaaaaaa tagccatatg aatgattaac gcaataaatg acaaccacca tacacctttt
    21721 gacagaactg ctacccatat tcctaaactt ctaaataata agatataggt tgataaaatc
    21781 atacctgcca taaaaaaagt agtaaagaca gaagcaatca aaggattgct taactccttc
    21841 ctgctttctt ttttattttt taatattcct ttgattaaaa aaatccaaaa aacaagtgct
    21901 actagcccta aacaatagcg aacataggtg ccatatccct ctagaaggtt ccccaaagct
    21961 aatagcccta atactaatcc cgccatcact aatggtggtt tttcccaatt tttcatcata
    22021 tacctcataa aaaaacgttt acatctatta taacacaatt agattttgct gataagtgat
    22081 tttattaact taatgaaaag aagctagtcc attgactaac ttctttttta agctcttcta
    22141 aagtttgctt ttattttaag tgacgattcc tctttaaaga gagacctact agagcaagta
    22201 gacttgctag aatgcttatt cctactacac ttagtatttt tgatgactta tctccagttt
    22261 ttggcaaatt agcttcattt gtcagatatg aatcggcctt cttagaaact acaggagtta
    22321 ttgaggtctt attagctatt ctatttactt tttctaccgt tttagattcc ttgaaagatt
    22381 gtgttttggg ataatctgct tgcaattcct ccttagggct aggagcaact ttgccaactt
    22441 taatcacaat tgctgttaat ggatcaatca gcaaactatc tttttcaaaa tgaacacctc
    22501 taggagttga gattggttta atccccgctt gatcagcatc aacaattact tgccctttta
    22561 aaaggtgttt atatttttca cctagtaaaa cgtttctagc tttactatca gcattaacaa
    22621 atactgcgta aatatcacct ttagaatcta ttgtttggta agcaataacc aaatcttttt
    22681 ctttaatatc accttgacct acctctgtaa tcaagctaac ctcacgatca atttctgctt
    22741 tgctcaattt ccggaaagca tctgttgaac gccttaatgt gattaaacct gctgtatagg
    22801 cctgtgtttt cgttgaaatt gggtgtttgt tattatctgt ggctgctgcc caatcaaaat
    22861 gattaatggc atctgaagaa tcatatgaat catgaataaa atatggatat tctttaacag
    22921 cttcaataag tgttgcttta ttaggcaatt tgtcatctga aacttttgtc atgtaatcag
    22981 ggttaagtaa acgcttcgta cgaccatact cttgaccaga atgaatgaat gctgtccctt
    23041 gagatgttaa aatcattaca tttcctaaac gcagacgtct atgaatctct tcttcagcta
    23101 ccttagggtc tttattaatt gattttgcaa tcacatcatg caaggtaagg ttatcatgtg
    23161 cagcaatata ctgcaccaca tctcctggcg aatctgcttc aaaattccca ggttgtgctt
    23221 tgatattttt aaaaatacct tgtaaagatt gtgggccacc tgtgatgaaa gctggagtac
    23281 cttcatttgg aaaaccagat ttcaagctat tacgaatatc atctgaaaag acgccaactg
    23341 tatcggttga cttcatccaa tcttggtcag ctggtttaac cggctgacct tgatcgcctt
    23401 ggaatgttct ccagccctca ccaatcataa tcatattagg attaatagct ttagcttctt
    23461 tataagctaa ttcaatcgca gccgcatcat gatctcccat catatcaaaa cggaaaccat
    23521 caactttaaa ttcacttgta agatatttaa tggaatcaac caaaacacga cgactcattg
    23581 catgagtggt tcctaaacgt ccccctccaa aactttctct tggtgaacca tcttcattca
    23641 taaagtgata ataattaggt tctatatcct caaagagata agtttttgca gtgtgattat
    23701 agacgacatc aagtataacc cccatgccac gtttatgaat atcatgtatt aattgtttta
    23761 attcggcgat acgtgctgat ggatcttttg gtttctctga atacatccca gaaagagcaa
    23821 aatagctctg tgggtcatag ccccaattgt aattattgtc tgaggaagtg taagctgttg
    23881 agcgtgactt atccatttca ttaacataaa aataactcaa taccggtaaa agctgaatgt
    23941 gtgtaactcc taatttctgt aaataatcta gtttctctga aaaggctgca aaggtaccaa
    24001 attgattttt taattttcca tccaaagatc gatcagaagt gaagtctctt acatgtgctt
    24061 cgtatataac agcatcttgt cttcctttaa aattagcaat tttagcaaaa cttaaatttt
    24121 gaggtccaag ttgacttgga tttacaaaag ctgctttagc cgttttaata tcatcattaa
    24181 cagtattact atcccactct gctaatgact ttgcataagg atctaaaatc ttaaccttat
    24241 ccttacctct ttttatttcg taaagataat agtaaccagt atagttttta atacctaatt
    24301 tagtatcaag tatcgtctgc caaacacctt tattattttt cacaaggggg gtagtcgcta
    24361 caaccctgtt ttggttatct ttgtcataaa taatcatagt gacactatca gcactcggtg
    24421 accagaggct ggcttcaact tttgaaccat cttgattgag aactgcacct aaatttccac
    24481 tataagcata aagttggtct ttaaattccc aagattgcct tgtcatgaca ttgttaccat
    24541 tataagatat gttgaaatga ccttgtttag gattaaagtc gcctttgatt attaaaagag
    24601 atttactagt atcgagagtg atatcagaaa tttgtatagc attttgattt ttatcagtca
    24661 ctttcaattc ttttaaaatt tcagttttat ctaccccatc tagagttgta aaacttgctt
    24721 gaatacttgt taaatcaatt tgttgggcat cctttagctg cacttgatca atgtaataag
    24781 gattattata aacctttgga tccttatctt ttacaaaaat ttggttatgg ttagctaaat
    24841 ctctaaaaac atagtcgttg ggttgaactt tcactgcatc tcctgtctta ctttcatcta
    24901 agattagaaa accaatctct ttggcgttag tttttagtga tacatcaata taccttccat
    24961 atagtccttg attcacaaaa ttactaccat ctggccaagt tgttgagggg gttgcaacat
    25021 ctttaaagag ccatgctgat aagtggtcat agttactaga ggaactcaaa tagttaatac
    25081 ggacataccc ttctttgagg ggttgataag tatgtatacc gtacttttca tcaatccaaa
    25141 cttggttcat ctcaggtcgt aataatggaa tatgatgatc gccgcttaaa tttgtccctg
    25201 ctttgttatt aattaaaaaa gatatttgtt ttcgttgttt ttcagataat ttaaaatcaa
    25261 cataataacc gtaatcatct ttcttagcat cagtcatagg gatagcacca tttggccaat
    25321 tactagacgg ttgatcaaca tctccccata tccaaagtcc agtgctttct acaggatgac
    25381 cttgaggcaa ttctttaaga tgtagcctaa agtaaccatt ttcaatccgt ggagcttgat
    25441 tagctacttt caaggtaggg cttggagcac ctatagcttg agaagttttg tcgggagtat
    25501 tttgcaatgt agatggtgct acttttaaat ctaaggtatt tttatctgaa acaggacctt
    25561 tattttcttc aagcaggctt gtcgccttat ccggctctgt cactcccttt tgagtgtcag
    25621 tttcatctga tactaattgc ttggtatccg gcatatctga gggtgtcaca accgtctcag
    25681 agtttgagtt tatgtcatct tttaaattag tagattgttg gttgtcaacc tgattaatat
    25741 tcgactctgc aatcgctgta gttgcaggag aaacacttac ttcttcagca aatacctgtg
    25801 gactatgaag aaaaatacat acccctgttg taactgaagc tactccaaca cttaactttc
    25861 taatcgtgta ttgagtttgt ttatcaccaa ataagtcttt tcttttcata tcattctcct
    25921 tttttaatga attgttacca ataaaatcat aacaatttac gcaagcgctt gcaacaagta
    25981 aatgatttct ttattttttt gaaattttca ctaaaactat caatagcatt acaaaaaagc
    26041 taagcaatat tgcctagctt ttttatcaaa aatttcctca tatcaaatga ttttcatcaa
    26101 tttgatagtc ctttagcaga ccttgaagtc tatcaaattc ataacaaaaa tgttcaagaa
    26161 tatgaccgtc agagcctata gagtattgtt tacaaccaag ctgtttgagt atctctaaag
    26221 catagcgata aagtttttca ttcccatata gataaaggga tttggtattt agttcaaaag
    26281 ctaacccctt agataacatc tttatgaaaa tgcgcttcaa ttgcgtttca aacggtttta
    26341 aatcttctac atctaagttt aacttacgaa aaccataatc aaagtgagct aaaacgtgcg
    26401 cttccacacg gcctatggca aattccatac gattaaagta atctgctaaa agctcctttg
    26461 ttggtacttt cagagcttct tcttgcagat aatcatacct accattatga tggattgaca
    26521 atagttttaa atcaaattct ttatttttta aataatctaa aatatctgat tccctatctt
    26581 taaaataacc gatttcaatt ccttttttaa atcgatttcc atatttctga ttaagataat
    26641 ctattttttg acaataagca ctataatcag gaacatcgtc ttgaccggtg taaggatttg
    26701 ataaatcaaa atgttctgtc gtgataaatt cacctgtaaa accattaata tagtcctcaa
    26761 atgccgtttg acaatcatag gaaaaatacg tgtgtagatg attatctctc ataccaactc
    26821 ctatccttaa tcttttaaaa tatctgctac tatcttttgt aaatcaacga ttagatcttt
    26881 ttcaaaccat ggattcttct ttagccaaat ttgatttcgc ggtgatgggt gaaccagggg
    26941 taaataatcg ggtagatagt ctttgtaagc tttaactgtt tctgttagat ttttatgtgc
    27001 ggagcttcca agataatatt tctgagcata ctgaccaact agcaaggtca attgaacatt
    27061 aggcatttct tttaaaataa gagggtgcca tttctccgca aaaccttttc tagggggtaa
    27121 atctcctgat tttcctttgc ctgggtaata aaaatctaaa ggtaaaacag caaattttcc
    27181 agaatggtaa aatgtctctt catcaactcc aagccactgg cgtagacgat ctccagattt
    27241 atctttccaa tagagtcttg cttcttgagt ttttaaacca ggtgcttggc caacaatatt
    27301 gatcctagct gttttaggag cagcaaacaa aggatcaata ccattttctg tataattttg
    27361 attctgtgaa tcacttttta ttgctttaat aattttttcc aagtcagtca taaagtctcc
    27421 aaatctaacc ttatgaaaag aggcaaagac actttcatgt cttcaccacc ctttatcatt
    27481 gatcacatat ttagtttaag gattatttta tttgattaat tcatagatag cttctgcgta
    27541 gatagcagcc gaacggaaaa tattttctaa aggcatgtac tcattagctt gatgcatagt
    27601 gttttcatct cctgggaaca tggcaccgta tgcaacaccc cgttcaagta agcgaccaaa
    27661 tgtcccacca ccaataacct gttcatgtcc tttaagacca gtttgctttt catagacagc
    27721 tagtaaggtt gatactaatt catcgtccat aggtacatag tgtggtgtgt gttcatggtc
    27781 agaaagagtc actttttcaa ctccgttaag tttctcaagc ttagtttgga tagttttagc
    27841 atctgtccct tgtgggtaac ggaaattaag agcgatagta ttatcatcat tagtttcatc
    27901 aaactggaag acacctgcat tcatgctcaa tggtcccata cagtcatctt cataagctac
    27961 tccaagtttt tcacctgaga agtcttcgtg aataatgttg gctgtaacat ctaagaaagc
    28021 acgagcagga ccttcaaaat caaattgact tagataaaga gctaaataag tcgccccatt
    28081 aacaccttct tgtggtgttg aaccatgagc agatttacca taaactgttg ctgtatattg
    28141 ctcatcttct acagataatt ctgctttcaa attatgttta cttgcaaaat cagctagact
    28201 agccccaagt gttgtctgat ctaaatgact tgtgaaacgt gctgtggctg attctggaac
    28261 catgttttcg cgaagtccgc cagaaaagct atgcagtcta acagctccct tattttctcc
    28321 tgaaaaatgt aaatattcag taatattacc tttttcacca tttataattg gaaattctgc
    28381 atctggagag aaaccaaaat caggttttgg aagaccaaca tgttcgaagt agtaatccat
    28441 atctccccat ccagattctt catcagttcc tacaacaaaa cgaactttct tagatgttgg
    28501 tagcccaagc tctttaataa tttttagagc ataataacat gccattgttg gtcccttatc
    28561 gtctgatgag cctcgagcat ataaacggtt atccttgata actggttcat aagggtctgt
    28621 atcccaaccg ctgcctgcag gaacaacatc taaatgccca aaaataccta gttcttcttc
    28681 gccttgacca aatgtaaagt gtcccgcata attatctacg ttttttgttt cgtagccatc
    28741 tcgctcagcc atctcaagga aaaattccaa ggcttttaca ggtccaggac caaatggatg
    28801 ctctgcatca gcttgtgaat catcacgctc cgagttaatg cgaagaagat ttattaaatc
    28861 gtccatcaaa gcgtctttgc gtttgtctac ttctgctcta aaatcaattg tcattacggc
    28921 aactccttct ataaaatatt atgtagtttg tgcttaaaaa ttaacgtttt tcaataatat
    28981 gatcaactgg caaacgataa ctaggttcaa ccttttcatc actataacca acagtaatta
    29041 atatttctgg tctaaaacgt ttatctatct ctaagacatc attagtaatc gctttatcga
    29101 accctaaaat catattagaa ctaatacctt gatctgtcaa ggcaagtact aaattcatag
    29161 caacgatacc tgcatttaaa gagagatact ctccagtttg tttttcacta tataaagcat
    29221 agcgtggtgg tagagtttcc atgtaataac caattaaatc atcaggaaga gatctacgac
    29281 caatacgagc tattttccgt gatctctgtc ctaaatctgt atctgtaaat aaagcaataa
    29341 catactgcgc ttgattaatt tggttacaat tactttccgg taaaccttct gctagagcag
    29401 attttttttc ttggacaacg acaaacttcc aaggctgaat attattagca gatggcgcta
    29461 atgtcgcaat ttcaatagca gttctaacat ctttaaagtc gactggttta tcattaaaat
    29521 gtttcacagc atggcgcttc ttatttaatt ctagaaattt cataaaaacc tcctattcta
    29581 tttgattttc cctactattc tatcactttt taaaatgcgt ttcagctttt gaaggcaaaa
    29641 ttgataaaaa agtgaaaccg gtttgaaaac taccataaaa aacaaacaaa cttgtggtac
    29701 aatatctaat gactataaaa aaggagctat tcatgctacg agcattaaaa gaagattttt
    29761 ttctaacctt aacctcttta aaaaatcaat tttgggttta cattggaact ggtatcttaa
    29821 tccaaacttt agttgcttat ttagtaaaag gaattctgag ttttatcttt cgtcggatat
    29881 tgatattgtc aaatactccc gcagtgacta aagacaattg gactttactt tttaatcatc
    29941 ccttagcttt attactcttt atactttata tcgtaatcct aattgggttc atctatactg
    30001 agttcgctat ctatactatt gccatactga aaactgaatt ttctctcagt atcaaaaaat
    30061 ggctaacaat ctttcctaaa aaaataaaat cacttttagg accacagcta atttgggtat
    30121 caatctactt acttttgaca attcctctag ctaaccttgg tctaaggtca agtatactag
    30181 agcatcttaa aattcctgac tttatttctg gcgagttaac aaagacagtc tttggaaaga
    30241 taggttactc taccctttta ctgcttattt gttacttaaa ccttcgatta atttattttc
    30301 tcccgctgac cattttaact gactacaatg ctaaagaagc acttttagag agttggaaac
    30361 ttagccgcgg aaagcatcag tggcgtcttc taagtaaaat tattttaacc agtttattta
    30421 tatctgtaat tggaacagtt gcactagctg ttgttgctgg tacatcttca ttcattgatc
    30481 attctggaaa caattttcct cttcaagcca ctttctataa ccttttaaaa tcaatacttt
    30541 ttgccgtaac acttcttatt aaatgtttaa tcataagtca cctattagca gatattaaac
    30601 aaaataaaag tattgttcaa caatggagag cttggaaagg cgtccaacat aagcaaaaat
    30661 ttaaacacat tgcttgtgtt atcctagctc tagctattgg taccactgcc ttaaaaaatg
    30721 ctatagctct ggctctccta caagataaca tttcaattaa aaaagaatta atagcccatc
    30781 gtggtgatac atcacatggc gttgaaaatt ctattgaagc attaaaagca gcccataaag
    30841 caaaagcaga ttattcagag atggatgtta tcatgacaaa agatcataaa ttagtagtca
    30901 tccatgatga taatctcaaa cgactctctg gtatgaacaa agatgtttct aaactaactt
    30961 tagatcaagt tacaaaaatc ccaattcacc aagggcgttt tgctagtcac attccatcat
    31021 ttgcagaatt tatgaagacc gctcagtcac tagatcaaaa aatcatgatt gaattgaaac
    31081 cgtataatca aaacctcgat atctatgcgg ataaatttat caaagaattt aaagagcttc
    31141 gtctatcaac caagcataaa gtaatgtctc tcaacttgac actcattgaa aaagttgaga
    31201 aaaaattgcc acaacttgat acaggttacc taatcccact acattgggga acgcttcaaa
    31261 atcataacgt tgacttttat gggattgagg aattttcata caatgattgg attgcatatc
    31321 tcgcccaaga atataataaa cagctctatg tttggactat taaccgtgat aaccttatga
    31381 tacgttattt acaaagccca gttaatggaa tcatcacaga tgaactcaac cttttcaagg
    31441 tcattaataa agatatcaaa aatagcccta attattatca aagagctctc caacttattg
    31501 atagcgaggg ttaaattaaa taaaaagctt agtagaacag tatctactaa gttttttatt
    31561 taggaggatc attaagagaa tctcggagac tttctgcaac agtcctagga ataccacaca
    31621 taataatgtc ctcaatctca gctttttgga tattaggtaa gcttttaaaa tgtttcatta
    31681 gcaattgttt acgttttggt cccaacccag tgataccatc taattttgaa gaaaaggaat
    31741 ttttggagcg taattgacga tgaaaagtaa tagcaaaacg atgtacctca tcttgaatac
    31801 ggtgaagtaa gaaaaattct tctgaattac gcggtaattc tactacttct aaaggatctc
    31861 caaaaagtaa ttcatgtgtt tggtgtttat cattcttttg caaacctgca ataggtatgg
    31921 ctaaacctaa ttggttctca ataacatcac gcgctatatt tacttgcccc tgaccaccat
    31981 caattacaat caagtccgga ggtgtcaagc catcttttaa aaccctacta taacgccgat
    32041 gaatgacttc tctcatacta gcataatcat ctggtccgat aacagtctta attttaaact
    32101 ttcgatagtc ttttttactt ggctttccat tcacaaatac caccatagca gctactggac
    32161 ttgtcccttg aatattggaa ttatcaaatg cttctatgcg tactggttta gggatattaa
    32221 gaagattacc taaattttca attgccccat gagttttacg aatatctttt tccagtaagt
    32281 caaacttctg ttgtaaactg acacgagcat tttttattgc cagatttact aactgtttct
    32341 tttcaccacg ttgtggttta acaatcttac agcctacaat tgtttcaacc gacttagcat
    32401 caatatcctg tggaataaag acttctttgg gtagaaaatg ttttgtatct tgataaaatt
    32461 gccctatgta agttagaaaa tcttcctctg gttcattata ataaggaaac atattcacgt
    32521 cacgctgaat caattttcca tttcgaacaa aaaagacctg tacacacatc caacccttat
    32581 caacgaaata accaaaaaca tctctatcct tcatatcttg atgaataaca cgctgcttag
    32641 tacgcaacaa actaattgct tcgatcaaat ctcgatattc agccgcacgc tcaaattcca
    32701 tagtcatagc agcgctcttc attttttcag ttaatccgtt aacaatttta ttatctttac
    32761 cattaagaaa ctgttttaca tcttctctca gactatccca ataagcttta ttagtttgac
    32821 aaactgtatg tgcattgcat tgccctaaat gataataaaa gcatacttta tttgctggat
    32881 tagtacattt cttaaaagga aagagcctat ctagtaaccg cttaatctca gttgctgccc
    32941 cagaatccgg ataaggtcca aaataagtgc catctgattt ttttacttga cgtgtaatca
    33001 taagacgagg ataacgttca tttgttattt tgatatatgg gtaagactta tcatctttca
    33061 atcgaatatt atacttcggc atattttctt ggattaaatt aatttccaat aatagagctt
    33121 cggtattaga tgttgtcaca atgtattcaa agtcttcaat ctcagagacc aaaagttctg
    33181 tcttagtatt atgactccca tgaaaataac ttttcacacg attcttaaga tttttagcct
    33241 tacctacata aataattgtc ccattcttat ctttatgaag gtaacaacca ggactgtctg
    33301 gtaacaattc tagcttatgt ttaatcagtt cattcataga actattataa caaaatgtac
    33361 tgtaatcttt acgaaataaa tactattccc caacatctat tgtatcttcc aatacttgaa
    33421 taaaacccca atactcctag tctaagcaat tattttatgt tacactttaa acaagtgaat
    33481 tattgaggag tttttatgat agacgttgag aatattcttg aaaaaatgaa acctaaccaa
    33541 aaaatcaatt acgattgggt catgcaacaa atggttaagc agtggcaagc tagtgatatc
    33601 cgtccgaaaa ttctgatgca tgtttgctgt gctccttgta gtacatatac ccttgaatac
    33661 ttatcacaat gggcagatgt taccatctat tttgctaatt caaatatcca tcctaaagat
    33721 gaatattatc gccgcgaata tgttactcaa aaatttgttc atgatttcaa caaaaatact
    33781 ggatattctg tacaattctt atcagcacca tatgaaccaa atgaattttt caaaattgtt
    33841 catgggttag aagaagaacc tgaaggtggg gatcgttgta aagtttgcta tgatttccgt
    33901 cttgataaga cagcggaaaa agccgttgaa ctaggttttg attactttgg cagtgcccta
    33961 actattagtc ctcacaaaaa ctctcaaaca atcaatacta ttggtattga tgtccaaaaa
    34021 atttatgata ctcagtatct tcctagcgac cttaaaaaaa ataaaggtta ccaacgttcc
    34081 gttgagatgt gtaaagatta tgatatctac cgacaatgtt actgtgggtg tatcttcggt
    34141 gctaaagatc aagggataaa tcttctccaa atcaaaaaag atgcgaaagc tttcgtgagt
    34201 gataaagacg gcaaagaaga atttcctaat atacgcttca catttaatgg caagtcaatg
    34261 taaatgataa aaaaagactt agtaaagaac taagcctttt tggtataaat cttgtcaaac
    34321 tttaataata caacttctta atcttttgca gacgttggtt tagaaataga ccacaaacaa
    34381 aaatacctgt gattaaacca atccaatagg caaacggacc taactggcta tttttttcta
    34441 agataaccgc taatggcaaa gcacataacc aataagagcc cgcaccgatc ataaatggtt
    34501 ttgttgtatc cttatagcct cgtaaaatcc cctgtacagg agctgcataa gcatctgcaa
    34561 actggaaaaa gagactataa gttaggaatt gagctgtaat agcgacaaag tgagggtcac
    34621 tattatacat tgctgctaca ttctcacgaa atagaaataa aaatagtaag gttcctgatg
    34681 taatccctac cgctgttaag cgtcctatcc tactataagt gtttgcgtcc tgaatgcgct
    34741 ctgcccctac ttcaaacgat attgtaatag ctagagcagt ggaaatactt aaaggaaagg
    34801 catacattaa tgatgaaaaa ttcatagcag cctgatgtgc tgcaataatg attgaagaaa
    34861 attttgccat gaataagcct actactgcaa aaattgcaac ttctgcaaaa atttgtaaac
    34921 caatcggtaa tcccaatcga atatcttcaa taatcaaagg agcttttatt ctttccagag
    34981 tccatatatg atatgtttta atttgaggat gaagtgacat cacaataata ataacaataa
    35041 aaatagccca ataagttaaa gaagttccaa gacctgcccc cgcacctcct agtctaggca
    35101 taccaaattt accgtagata agcatataat taaaaaatga attaaagggt agaattaaaa
    35161 gcatcagata catagataac cttgttaacc ccaatgcatc aaagaatgaa cggcaaatgc
    35221 taaacaacac cagcggcatg attccaatca acatataatt taaataacca cgaccaactg
    35281 ctagaacttc atcttctaaa cccaaactcc ccaagacagg ttgagcaata aattgcatga
    35341 ttaaaaataa tattaaggac agtatcaaac ctaaatatag aaattgatga aattctgtgc
    35401 gaatttgttc tttatttcct ctacccaaat gttgaccaac tactggtact aatgctgaaa
    35461 tcatacctac taataaagcg aaaaacggag tccataaatt actagcagtt gacacacctg
    35521 ccaaatgtag ctgactatac tgtccagtca taaccgaatc aataaaagta gctgaaaaat
    35581 tagcaaattg ataaatcaat actgggataa ataatttcaa aaataaaaca aatttttcct
    35641 tatttgtctg agtttgatac atcttttttc tccttttcac cttaatcaat ttattttagc
    35701 ttagctagga tactctgtca atgctattta aaaaggttga gaaataatcc tcaaccccat
    35761 tttagctaag ctcagtattg atttgccatg tcataccata tttgtcagtc acatttccat
    35821 aagctggact ccacggaact gcttgtagag gcattttaat gttcattgca tctcgagaaa
    35881 gcttattgaa taactcttgc gcttcttcta cagattccac aattaaagct gctgtcatat
    35941 tttgtccaat ttgaaaatca aaaataggat tattgtcaga aagttgcatt cgaataccat
    36001 caatattaaa ttgagcgttc ataattaaat gttcaatttc tggcggacaa tctggccaac
    36061 cctgtgcaaa agttgtgacg tgttctgctt ttgtattaaa agcctcttca taaaatgtaa
    36121 tagcttcggc cccacgacca ttaatattca tataaatttc aattgctttc attaccatac
    36181 ctcacttcat attatccaac caacgctgca tcagagtttc attttcccct tttagggtaa
    36241 aataagaacc acgtattata cctgcgagtt ttgacttttg acgagcaaac ttaaacttgt
    36301 ccgctaattc aataggaata tccattccat ctgataattt taaacctaca gctctctttt
    36361 taggattatc taattcatat aaaatattaa tggctaaacc atcctcatag aaaacatgtg
    36421 tccataaaat acgattctct aaaaaaagct gtgaaatctc taaaggcttt gcagcaaaaa
    36481 caaggttacg ttcatttttt agaaggtgat taatatctgc catcaattct ggatagtctg
    36541 atactggaaa tgtttcgtag gctactttga atttattcca gaaatagcga gcttcatttg
    36601 cacgcaaacc agccaaggct tctgccactg gagaagtctc caaaccttta gttgaaacgg
    36661 ttttaaagtc gataatcatg ttctttcctt tttgattaaa atcttcgata atttagtata
    36721 ctcttttttg aagaaaaagt acattctaat cacttataaa aagtagtaca tttagaacac
    36781 taccacctca gtaatttctt gacctttctc tacttaatac ttataattaa tctagactta
    36841 atgtcaaagc atcaatcaag gtgtaaacct gattcaccta aaaagctgat ggagctaaac
    36901 atgaccaaaa agatattctt atttatctgc gctactcttt tagtaggaat agccttatat
    36961 acaccaacaa cttctatttt taatcataac aacgtttatg ctgccaccaa gaaagcaaaa
    37021 attaaattta ataaaactca aaaaaaaatt gtcaaaaaag caagagaata tgctaagtct
    37081 ggccatatgt ctaaagatag tatcattgaa aaattaaaaa aggattctaa gaaatatcgt
    37141 caagaagaca tcaactttgt gattaataac ctcaaggttg attacaaaaa aaatgccttg
    37201 atttctgcca aaatttattc aaagaccatg aatttatcta aacaatctat ctttgaacag
    37261 ctctattcgg agtctccaga taaggcaacc cactctgata aatttacaaa agaagaatcc
    37321 caatatgcta tagaccatct caaagtagat ttcaaagaaa atgctcttga gactgctaaa
    37381 agttatcaaa gtagttcttc tttatccaaa gaagaaatct ataaacaatt gacatctacg
    37441 ctgggcgata aattcacaaa tgatgaagcc caatatgctg tagatcattt gaaatgatga
    37501 cataccaata gtctatcctt atgtaataga attgatagct tatgctaata ttccagctgt
    37561 aaaatagcaa gaaccctcct aagttcatta agacttagga gggtttttat caatatgaat
    37621 aggttacact aaactagaca gaaattataa agtgttctac acttaagaaa aactggagaa
    37681 aaaccatgtc tagaaaagta cgtcgtcact tcaccgatga ttttaagcaa caaattggtg
    37741 acctttacaa tgttggtaga aagcgtagca gtctcatcaa ggaatatgag ctaacccctt
    37801 ccaccttcga taagtgggtt agacaagcca aaacaactgg ttcattcaag tctgttgata
    37861 atctgacaga tgaacagcgg gagctaattg aactcagaaa acacaataaa gaactcgaaa
    37921 tgcaattaga tatcctaaag caagcagcgg tgattatggc acaaaaaggg aaataatcac
    37981 tgctaataag gataaataca gcatttcaaa gatgtgtcgc tggctgaata tgccacgctc
    38041 aagttattac tatcaagccg tggagtcagt atctgaaacg gagtttgaag aaactattaa
    38101 aagaattttt ctcgatagcg agtctagata cggatccaga aaaatcaaaa tatgcttgaa
    38161 taacgaaggt atcacacttt cacgtcgtcg gattcgacgc attatgaagc gactcaattt
    38221 ggtttctgtt tatcagaaag ccaccttcaa accacattct agaggcaaga atgaagcccc
    38281 tattcccaac cacttagaca ggcaatttaa gcaagaaaga ccactacagg ccttagtcac
    38341 tgacttaacc tatgttcgtg caggcaatcg ttgggcttat gtttgcctca tcattgacct
//
LOCUS       DATRSD010000008        34876 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRSD010000008 DATRSD010000000
VERSION     DATRSD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34876
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.008"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..844
                     /locus_tag="VBD85_000331"
     CDS             <1..844
                     /locus_tag="VBD85_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132906.1"
                     /translation="FVYSVKRLPYVPQVIMTTTSNVLMPQKQVEKVKLLTPFTISNKE
                     VLNHDSLVENDAQKQKVEQEKTKSLAPQKGAVKEQTEQKVSGNTQEIEKKSETVATPQ
                     QSSVAQTSVQQPAPVQSVVQESKASQEEINAAHDAISAYKSTVNIANTAGVTTAEMTT
                     LINTQTSNLSDVEKALGNNKVNNGAVNVLREDTARLENMIWNRAYQAIEEFNVARNTY
                     NNQIKTETVPVDNDIEAILAGSQAKISHLDNRIGARHMDQAFVASLLEVTEMSKSISS
                     RIKE"
     gene            911..1456
                     /locus_tag="VBD85_000332"
     CDS             911..1456
                     /locus_tag="VBD85_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132907.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEMPTRYVKMSEKSKAFY
                     QRLQEKQRKAHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQDNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1607..1696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1852..2418
                     /gene="thiW"
                     /locus_tag="VBD85_000333"
     CDS             1852..2418
                     /gene="thiW"
                     /locus_tag="VBD85_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO7132908.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2415..2969
                     /locus_tag="VBD85_000334"
     CDS             2415..2969
                     /locus_tag="VBD85_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7132909.1"
                     /translation="MKLSLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHERK"
     gene            2973..4259
                     /locus_tag="VBD85_000335"
     CDS             2973..4259
                     /locus_tag="VBD85_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7132910.1"
                     /translation="MLSVEKLACTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKRLTRVNFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSL
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4247..4915
                     /locus_tag="VBD85_000336"
     CDS             4247..4915
                     /locus_tag="VBD85_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO7132911.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLSIG
                     MIFK"
     gene            4999..5679
                     /gene="tenA"
                     /locus_tag="VBD85_000337"
     CDS             4999..5679
                     /gene="tenA"
                     /locus_tag="VBD85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO7132912.1"
                     /translation="MLKDKMIAKGAKYIQSIYQDPFIQGIIKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQIDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKGVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5681..6478
                     /gene="thiD"
                     /locus_tag="VBD85_000338"
     CDS             5681..6478
                     /gene="thiD"
                     /locus_tag="VBD85_000338"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7132913.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6480..7250
                     /gene="thiM"
                     /locus_tag="VBD85_000339"
     CDS             6480..7250
                     /gene="thiM"
                     /locus_tag="VBD85_000339"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO7132914.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYSISLLRGNGSEIAALIGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7264..7935
                     /locus_tag="VBD85_000340"
     CDS             7264..7935
                     /locus_tag="VBD85_000340"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO7132915.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMDEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8062..9321
                     /locus_tag="VBD85_000341"
     CDS             8062..9321
                     /locus_tag="VBD85_000341"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7132916.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPCGVKDIPMLYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTIIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQERLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9423..9977
                     /locus_tag="VBD85_000342"
     CDS             9423..9977
                     /locus_tag="VBD85_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7132917.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFREFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            9970..11253
                     /locus_tag="VBD85_000343"
     CDS             9970..11253
                     /locus_tag="VBD85_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7132918.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGIEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTDLYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11276..12136
                     /locus_tag="VBD85_000344"
     CDS             11276..12136
                     /locus_tag="VBD85_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO7132919.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12138..13058
                     /locus_tag="VBD85_000345"
     CDS             12138..13058
                     /locus_tag="VBD85_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7132920.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13075..13530)
                     /locus_tag="VBD85_000346"
     CDS             complement(13075..13530)
                     /locus_tag="VBD85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO7132921.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQLQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13601..13714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13712..14221
                     /locus_tag="VBD85_000347"
     CDS             13712..14221
                     /locus_tag="VBD85_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO7132922.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            14361..16319
                     /gene="ligA"
                     /locus_tag="VBD85_000348"
     CDS             14361..16319
                     /gene="ligA"
                     /locus_tag="VBD85_000348"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO7132923.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEK
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     AYMAELKIDGLSVSLTYVNGVLQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRVSLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEGEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIENLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16331..17350
                     /locus_tag="VBD85_000349"
     CDS             16331..17350
                     /locus_tag="VBD85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7132924.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEATRAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVDDL
                     EGLGD"
     gene            17354..19654
                     /gene="pulA"
                     /locus_tag="VBD85_000350"
     CDS             17354..19654
                     /gene="pulA"
                     /locus_tag="VBD85_000350"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO7132925.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTQGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYHGKFKGLHQKGTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQNSFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            19860..21728
                     /gene="glgB"
                     /locus_tag="VBD85_000351"
     CDS             19860..21728
                     /gene="glgB"
                     /locus_tag="VBD85_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO7132926.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKNQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            21770..22909
                     /locus_tag="VBD85_000352"
     CDS             21770..22909
                     /locus_tag="VBD85_000352"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO7132927.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMNYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            22899..24032
                     /gene="glgD"
                     /locus_tag="VBD85_000353"
     CDS             22899..24032
                     /gene="glgD"
                     /locus_tag="VBD85_000353"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEO7132928.1"
                     /translation="MKIDKYTAILGNTVSYPDMHSLTDNRPLASLPFDGKYRLIDFQL
                     SSLANAGVRSIYGIFRGNNVRSVFDHIRSGREWGLGTLLSHYSLGFYDTDDDTRTVNG
                     DYYDQILTYLRRSGSDQTIYMSCDMLCNIDLSQVIHLHNANGAQATVVYKKMPRSAIA
                     EANQVLTIDESDHVVSLGKAVDNDDNQKMSAEIYVIDTPWLIEKMEEEAQNNEPRKLR
                     FLLRDLIVESNALAFEYTGYLSNISSIKSYYDANMDMLTPNKFYSLFFSNQKVYTKVK
                     NEEATYFDKQSNVSNSQLASGSIIKGYLDHSIVSRNCLLEKGTRVVNSIIFPKVKIGE
                     GATIENTIIDKCVKVASGVTLKGSLDKPLVIPKFSEINEDIIQ"
     gene            24029..25459
                     /gene="glgA"
                     /locus_tag="VBD85_000354"
     CDS             24029..25459
                     /gene="glgA"
                     /locus_tag="VBD85_000354"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO7132929.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLRLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            25791..25991
                     /locus_tag="VBD85_000355"
     CDS             25791..25991
                     /locus_tag="VBD85_000355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO7132930.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            26024..26740
                     /gene="atpB"
                     /locus_tag="VBD85_000356"
     CDS             26024..26740
                     /gene="atpB"
                     /locus_tag="VBD85_000356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO7132931.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            26758..27255
                     /gene="atpF"
                     /locus_tag="VBD85_000357"
     CDS             26758..27255
                     /gene="atpF"
                     /locus_tag="VBD85_000357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO7132932.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            27255..27791
                     /locus_tag="VBD85_000358"
     CDS             27255..27791
                     /locus_tag="VBD85_000358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO7132933.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEKIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSIDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            27807..29312
                     /locus_tag="VBD85_000359"
     CDS             27807..29312
                     /locus_tag="VBD85_000359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO7132934.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            29328..30209
                     /locus_tag="VBD85_000360"
     CDS             29328..30209
                     /locus_tag="VBD85_000360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO7132935.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLSSRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLISQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            30283..31689
                     /gene="atpD"
                     /locus_tag="VBD85_000361"
     CDS             30283..31689
                     /gene="atpD"
                     /locus_tag="VBD85_000361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO7132936.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            31702..32115
                     /locus_tag="VBD85_000362"
     CDS             31702..32115
                     /locus_tag="VBD85_000362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO7132937.1"
                     /translation="MAQLTVQVVTPDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LNVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            32180..32410
                     /locus_tag="VBD85_000363"
     CDS             32180..32410
                     /locus_tag="VBD85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO7132938.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            32473..33744
                     /gene="murA"
                     /locus_tag="VBD85_000364"
     CDS             32473..33744
                     /gene="murA"
                     /locus_tag="VBD85_000364"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7132939.1"
                     /translation="MDKIIVEGGQTQLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            33746..33937
                     /locus_tag="VBD85_000365"
     CDS             33746..33937
                     /locus_tag="VBD85_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7132940.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            34012..34869
                     /locus_tag="VBD85_000366"
     CDS             34012..34869
                     /locus_tag="VBD85_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7132941.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNVSGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
BASE COUNT        11296 a         5347 c         7144 g        11089 t
ORIGIN      
        1 gtttgtttat tcagtcaaaa gattacctta cgtcccgcaa gtaattatga caacaacgtc
       61 taatgtactt atgcctcaaa agcaagttga aaaagtgaag ctactcaccc catttacaat
      121 ttctaataaa gaagtattga atcatgactc actagtagaa aatgatgctc aaaaacaaaa
      181 agtagaacag gaaaaaacta aatcgctagc tcctcaaaaa ggggctgtaa aagaacaaac
      241 tgagcaaaaa gtatctggaa atactcaaga gatagaaaag aaatctgaaa ctgtggcaac
      301 tccacaacaa agttcagttg cgcaaacttc tgtccaacag ccggctccgg ttcaatcagt
      361 tgttcaagaa tccaaagctt ctcaagagga gattaatgca gcacacgatg ctatttcggc
      421 gtataaatca acagtcaata ttgctaatac agccggtgta acaactgcgg aaatgaccac
      481 gctcattaat actcaaactt ctaatctttc tgatgttgag aaagctttag gaaataataa
      541 ggttaataat ggtgcagtca atgtattgag agaagataca gctcgtcttg agaatatgat
      601 ttggaatcgt gcttaccaag ctattgaaga attcaacgtc gctcgtaata cttataataa
      661 ccaaatcaag acagaaacag ttccagttga taatgatatt gaagctattt tagcaggttc
      721 tcaagctaaa attagccatt tggacaatcg tatcggagcg cgccacatgg atcaagcttt
      781 tgtagctagt ttattagaag ttactgagat gagtaaatca atctcatcgc gtataaaaga
      841 gtagacactg ctatcaaggc gatcttaaac ttttgtatta aactaaccta aaagatagaa
      901 agagactaat atgaaaaaaa taacaacttt aatcttagct agtagcttat tactagttgc
      961 aacgacatcg gttaaagctg atgataactt tgaaatgcca acgcgttatg ttaaaatgag
     1021 tgaaaaatca aaagcatttt atcaaagact acaagaaaaa caacgtaagg cacatactac
     1081 tgtgaagact tttaataatt cagaaataag gcatcaacta cctcttaaac aagaaaaggc
     1141 tagaaatgat atctacaatt taggcattct tatttctcag gagtctaaag ggttcatcca
     1201 acgtattgat aatgcctatt ctttggaaaa tgtctcagat attgttaatg aagctcaggc
     1261 tttgtataaa cgtaactatg atttatttga aaaaatcaaa tctacacgtg ataaggttca
     1321 agtcttactt gcatcgcatc aagataatac agacttaaaa aacttttatg ctgagttgga
     1381 cgatatgtat gaacatgttt atctcaatga aagtagagtg gaggcgataa acagaaatat
     1441 ccaaaaatat aattagtttc taaactaaca aacattccta aatataagac ataaaaccct
     1501 actcattgat tagtgagtag ggttttactg ttttaaatag ctttctgatc agaatgtaag
     1561 ccttgtcatt tcaaaggaac tatgttatta ttcttaagta aattaaatag gacatttggg
     1621 gtgcgtaaca gctgagatta tacccattga acttgatgca gttagtactg tcgaagggaa
     1681 atgtgtgcca ttacaagttt tttgtggtgg aaaagggcgt agtgtcttct tagtagacag
     1741 ctagctctag ttaatcagat aataaactcc gtttgcttgc agatggagtt ttttgttgtc
     1801 aaaatttttt gtaggttgtt gccccttatc tgtcatagag aaggagttat gatgactaat
     1861 cataaaacaa aaaaattaac ggttttagca attatgattg ctttagatgt ttttttaacg
     1921 cccattttta gaatagaagg catggccttt atgtctagtg tggttaatat tatcgctgga
     1981 gttataatgg gaccaatcta tgcattagtt atggcacttg taacggcttt tattcgtatt
     2041 ttaactcaag caggagtagc atctgttgca cctttagcta ttctaggagc tggtccagga
     2101 gctttcttag cgggttggtt ttatcggttt tatgcgaaag actggatgtc ttgggtgggt
     2161 gagtttatag gaacagggat aattggagca cttttatcag ctccaattat ggtctggttt
     2221 tggacttgga cagcggatgg aaatcacgaa gtgttagcta aagcatctgc agctcaatcc
     2281 atttttcttt ttatgccaag gtttataggg gcgactataa ttggcggtgt tattgctttg
     2341 ttgctacttc agggattaaa gcgggttcct gtatttatga caatacaagc attatttaaa
     2401 ggagaaaaag taaaatgaaa ttgagtttaa aagatgtatt attgattgct ctattagcgg
     2461 ttgttctggg cgttgtttac tttggagcag gctatatttc aaatgctttt gtaccttttg
     2521 taggccctat agcgcatgaa gttatttatg gtatttggtt tgtggcaggt ccaatggctc
     2581 tttatatatt gaggaaacca ggaaccgcta ttgtagcaga attgttagct gctttgatag
     2641 aggttttaat tggatcaatt tatggaccaa gtgtactcgt tattggtacc cttcaaggtt
     2701 tagggtcaga attaggcttt acactattta ggtatcataa ttacaagttg ccagctttta
     2761 tattgtctgc tattttgaca tcaattttta gttttgcatg gagtttttat gccaatggac
     2821 ttagtgcgtt ttcattttca tataacattc tgatgcttat agtaagaact gtttcatcta
     2881 ttattttctt cttattaaca aagaatatat gcgaccaatt gcatagaagt ggtgtactga
     2941 atgcttatgg gattcatgaa aggaagtagc ctatgctatc tgttgaaaaa ttagcttgca
     3001 cccatggcga tagtcattat ttatttgatg aagtgacttt tagtcttaac ccaggagaac
     3061 gaattttaat cagtggctat tcagggtgtg gtaagagtac attggctctt ttactatcag
     3121 ggttaaaaga aagtgggaaa ggtcaagttt tattaaacgg ctcattaata gaaccaagcg
     3181 atgtggggtt tctttttcaa aatcctgatt tgcaattttg tatggatact gtggcgcatg
     3241 agttgtattt tattttagaa aatttacaaa tagaaccgga gcaaatgcaa gatagaagtg
     3301 agtttgtatt ggcacaagtt ggtttaaaag gatttcagaa tcggttgatt tatactttat
     3361 ctcaaggtga aaaacagcga ttagcattgg cgaccatttt tttaaaatct cctaaactta
     3421 ttattttaga tgaagctttt gcgaatttgg atcaagagag tgccagtcaa ctattacaat
     3481 tagttcttaa ttatcaagca aacaatcaat caatgctgat tgtaatcgat catttgatta
     3541 cttattatca agatataatg gatcactatt tttggttgga aaaacggttg acaagggtca
     3601 attttgatta tatgctcaat cgtttgaatg tcttcgaatt agaaaagaaa agtcataata
     3661 caggtgacaa actgttatca attaaagatt tccaagttaa actttcaaaa aacaaattca
     3721 tctcttacct tgattttgat ttagcaagtg gggagaggtt gtgcttagat ggaccaagtg
     3781 gcgtggggaa gtctagtttg tttatgggat tgttagggct ttataggact aaagggaaaa
     3841 aacaatttac tcatagaaag caaattccta taagcttttt atttcaaaat cccttagacc
     3901 aatttatttt ttcaacagtt tatgacgaaa tttttcaggt ttgcaaggat agtaacaaag
     3961 cgagggatat tttggaaacc ataaatcttt gggataagaa acagttttca ccatttcaac
     4021 taagtcaagg ccaacagagg cgattagcca ttggcagtat tttagctagt gatagtaaac
     4081 ttctattatt agatgaacca acttatggac aagatgccta tcacgctaat atgattacaa
     4141 ctcttctttt gagttattgt cacaaaaatc attgtggggt tatttttact agccatgatc
     4201 ctcacttaaa agagtgtttt gcaacacgtt tcttggaggt gtcatcttgt tcaatttgaa
     4261 aaactatcat ccagctatta ccttcctatg tttattaata ccaacctttt cgtttagttt
     4321 cacattgagg ttaagaacgt ctttgttatt tctaattata gtagtaacac tgcaatgctt
     4381 cgtaaaggtt tctttaaaaa cttgggcgaa ggtcaatctg atatcatttg tgatgggact
     4441 ctctttattt ctagggacgt atttttgggg aaaattaccc catcaattcg tcttagctag
     4501 tctagtggcc tgtcgtccgc ttatttttat gaacgtgggt ttgttatttc atgcatcaca
     4561 ttctaattat gattttatag aatctcttta tcaaactttc aaagtgccta gtcattttgc
     4621 atatggtatt tttgcggtct ttaatcttct accattaatt aaattacaat accaacgtaa
     4681 tcgactcgca ttccgactga agaatcaggt aacttgggca ttatcgccta gactaatatt
     4741 gtcagtttta ttaaagacga tatattgggt agaacagtta gagttagcaa tgctttctaa
     4801 aggttttgaa gtgggtaaag agcgaacaca tgcttcaact tatcccgttc gttttcgaga
     4861 ttatagttta cttggtatga gtattttatt gtctatagga atgattttta aataagtaca
     4921 attagtttag taatacaatt gtgcatttat atagaatgag cgtgttctgc tcatgagatc
     4981 ataacaaagg agattactat gttaaaagat aagatgattg ctaaaggagc aaaatatatc
     5041 caatccatct atcaagatcc atttattcaa ggaattataa aagggcgttt agaccatgac
     5101 gtaatctgcc attatttaca ggcagataat atctaccttg ggaaatttgc tgatatttat
     5161 gccctgtgct tggcaaaatc tgataattta cgagataaac aatttttcct tgaacaaatt
     5221 gactttacat taaatcggga gttagcagat ggtgaaggcc cacatcaagc cttggccgct
     5281 tatactaatc gatcttatca ggatattatc gaaaagggtg tatggtaccc gtcagcagat
     5341 cattacatta aacatatgta ttttcatttt tacgaaaatg gtattgctgg tgctttagct
     5401 gcaatgagcc catgtccatg gatttaccat caattggcta aaaaaataat tgaggagaac
     5461 cagtttttga atggtaaccc ttttaataat tggataactt tttatgctaa cgatacagtt
     5521 gaagagttaa tggaaaacta ttttcgtatg atggattatt atgcgcagaa tttgtcaaaa
     5581 gagaagcaag ctgatttggt agatgctttt gtgaaatctt gtcagcatga gagacgtttt
     5641 tttcaaatgg caataaatca agaaaaatgg gaggattaat atgacttatt tagcaccagt
     5701 tttaactatt gcaggaactg atcctagcgg tggagcaggg attatggcgg atttaaagac
     5761 atttcaagct cgtcggactt acgggatggc tgttgtgact agtgttgttg ctcagaacac
     5821 ttgtggggtt agaggcgttc agcatataga aacagctatc attgatcaac aactagcttg
     5881 tgtctatgat gatattaagc caaaggcggt taaaacagga atgttagcgg agagggaaac
     5941 aattagttta gtcgcttcat atttaaaaaa atatccgcaa ccttatgtat tagatcctgt
     6001 tatggtggca actagcggac atcgtttaat tgattcagat gccgtagaag cattaaaaga
     6061 agacttattg ccattagcaa ctattatcac accaaattta ccagaagctg aggttttagt
     6121 gggatatgac ttatcagatg aagtaagtat tattaaagca ggttacgata ttcaaaaaca
     6181 atattctgtt agaaatgttc ttataaaggg aggacatcta gatggcttgg ctaaagatta
     6241 tttatttttg gaaaaagaag ggttgataac tcttagtaat caacgtatca atactattca
     6301 tactcatggg actgggtgta catttgcagc agtcgttgca gcggagttag ctaaagggca
     6361 atcaattcta aacgcggtaa gtaccgctaa atcatttatt acttcagcta tcgaaacagc
     6421 gcctgagttg ggccttggaa acggtcctgt caatcatact agctatcaag gagactaaaa
     6481 tgtatttaga acaattaaaa gaggtaaatc ctttaaccat ttgtatcact aataatgtgg
     6541 tcaaaaactt tacagccaat ggtttattag ctttaggagc ttctccggct atgagcgagt
     6601 gcatagagga cttagaagac ttgttaaaag ttgcagatgc attattaatt aatataggaa
     6661 cactaacaaa agaaagttgg caactttacc aagaagcaat caaaatagca aataaaaacc
     6721 aagttccagt tgtattagat cctgtagcgg caggagcaag tcgttttcga ttagaagtgt
     6781 cacttgattt attgaagaat tattctatta gtttacttag aggaaatggt agtgaaatcg
     6841 ctgctttgat tggtgaaaaa caagcgtcta aaggggcaga tggcggaaaa gtggctgatt
     6901 tagaatcaat tgctgtaaaa gccaatcaag tttttgatgt gccagtggtt gtcacaggag
     6961 aaactgatgc tatcgctgtt agaggagaag ttcgcttatt acaaaatggt agtcctttaa
     7021 tgccacttgt tacaggaact ggatgtctat taggggcagt attggctgct tttattggta
     7081 gttctgacag aagcgatgat cttgcttgtc tgacagaggc catgactgtt tataatgttg
     7141 ctggagaaat tgcagagaag gtggcaaaag gtaagggggt gggaagtttc caagttgcat
     7201 ttttagatgc cctttcccag atgaaatcag aaatgattat ggacaaatag gtggtgtcag
     7261 tagatgaaag atacattgaa attatatttt gtttgtggaa ctgtggattg ctctaggaag
     7321 aatatattga ctgttgttga agaagcctta caagcaggta taactctatt tcaatttaga
     7381 gaaaaaggtt ttacagcatt acaaggtaag gaaaaaattg ctatggctaa gcaactccaa
     7441 atactttgta agcaatatca agttcctttt attattgatg atgacattga cttagttgag
     7501 ttaattgatg ctgatggtct tcatattgga caaaatgatt tacctgttga tgaggcacgg
     7561 agacgtttac cagataaaat catcggttta tctgtgagta caatggatga atatcagaaa
     7621 tcacaattat ctgttgttga ttacatcgga attggtcctt ttaatccgac acaatcaaaa
     7681 gcagatgcta agcctgctgt tggcaatagg acaacaaagg cagttagaga aattaatcaa
     7741 gatattccaa tcgttgcaat aggcggaatc actagtgatt ttgttcatga tattatagaa
     7801 tcaggtgcag atgggattgc tgttatatcg gctatttcaa aagcaaatca tattgtagat
     7861 gcaaccagac aattgcgtta tgaagttgaa aaagctctgg ttaataggca aaaacacagt
     7921 gatgtaatca aataaggcta catcgttaat tataagaatt tttaagatat tatatcatca
     7981 cacaatactg gtatttgaaa aagaatttaa actatgataa tatagtagag ttgaattcta
     8041 gttaaagaaa gatttgaaag tatgcgtaaa attattatta acggtggcaa gcaactgacg
     8101 ggagaagttg ctgtatcggg tgccaaaaat agtgtagttg ccttgattcc tgccactatt
     8161 cttgctgatg atgttgtggt cttagatgga gttccggcta tttctgatgt tgacagttta
     8221 gttgacatta tggaaactat gggagctaag attaagcgtt atggtgagac gcttgaaatt
     8281 gatccatgtg gcgttaaaga tattccaatg ctttatggca aaatcaatag tcttcgtgca
     8341 tcctattatt tctatggaag cttattgggg cgttatggtc aagcaacatt aggtttacct
     8401 ggtggttgtg atttaggccc ccgtccgatt gatttacatt taaaagcttt tgaagcgatg
     8461 ggagctagtg tttcttatga aggtgattca atgcgtctag caacaaacgg aaagcctttg
     8521 caaggagcaa atatttatat ggatactgtc agtgttggtg cgacaattaa cactataatc
     8581 gcagcagcta aggcaaatgg ccgtacggtt attgaaaatg ctgcacgtga accagaaatt
     8641 attgacgtgg ctactctttt aaataatatg ggagctcata ttcgtggtgc tgggactgat
     8701 gttattacaa ttgaaggtgt gaaatcgctc catggcacgc gtcatcaagt aattccagat
     8761 cgtattgaag caggaactta tattgctatg gcagcagcta tcggacgagg aataaaagtg
     8821 accaatgtgt tatatgaaca ccttgagagt ttcattgcta agttagatga gatgggagtt
     8881 cgaatgacag ttgaagagga ttcaattttt gtcgaagaac aagaaaggtt aaaagctgta
     8941 tccataaaaa cctcacctta cccaggattt gctacagatt tacaacaacc tctaactcct
     9001 ttattactaa cagcagaagg aaacgggagt ttattagata ctatctatga gaaacgtgtt
     9061 aatcatgttc ccgaattagc acgtatggga gctaatattt ctaccctagg gggcaaaata
     9121 gtttatagtg gtcctaatca attatctggt gcacctgtaa aagcgacaga tttgcgtgca
     9181 ggcgctgctt tagttatcgc tggcttaatg gcagagggac gaactgaaat tacgaatatc
     9241 gagtttattt tacgtggata ttctaatatc atcgagaaac taacatcatt gggtgcggat
     9301 atccaattgg ttgaggaata gtatatataa caaagagttg agaatatttc ttagctcttt
     9361 tcttcttgtt ttagattaag tcacttatag gtatttgaaa tctcttgtgg tataatatta
     9421 tcatggatat atggacaaat ttaggacggt ttgcattcat tgaaacagaa catgttaacc
     9481 tacgtcctgt tgcctacact gatagggaag ctttttggcg aatagcttca aaaaggacaa
     9541 atttacaatt tatttttcct gttcaaacca gtaagaagga aagtgatttt cttttagttc
     9601 attcttttat gaaagagcca ttgggagttt gggcgattga agataaagtt agtcataaaa
     9661 tgtttggagt tatccgtttt gaaaacattg atttatctaa aaagacagct gaaattggct
     9721 attttttgaa agaaagttct tggggtcaag gcattatgac tgaatgtcta aagacacttt
     9781 ctttttttgc ttttcgagag tttgggatgg acaaattgat catagtaaca cataaagaga
     9841 atattgctag tcagaaggtt gctttgaagg cgcatttcaa acaatctagg agttttaaag
     9901 gaagcgaccg ttacacacgc cgtataagag actatattga gtttcaatta actaggggtg
     9961 attatcgtta tgagtaaaca tcaagaaatt ttggagtacc tagaaaattt agctgttggt
    10021 aagagggtta gtgtacgcag tatttcaaat catttaaagg tgagtgatgg cactgcatac
    10081 cgcgctataa aagaggcaga aaataggggt attgttgaga ctagaccgcg tagtgggaca
    10141 gtacgtgttg ctcaaaaagc aaaggttaat attgaaaaac tgacttatgc tgaaattgca
    10201 aggattagtg actcacaagt tgttgcagga atagagggac tcagcaagga gtttagtaaa
    10261 ttttctatag gtgcaatgac tcacagaaat attgaaaaat atttggtgca aggagggtta
    10321 ttgattgttg gcgaccgtga tgagatacaa catttagcct tacaacatca aaacgctatc
    10381 ttagttacag gtggctttaa tgtttcccca tctgtttgtc gtttagcaga taaacttcaa
    10441 attcccgtaa tggtaacaca ctatgataca ttcacagtat caacgatgat taatcacact
    10501 ttgtcaaatg ccaaaattcg tactgattta aagaccgttg aacaagtcta tcagtcgcaa
    10561 atggattacg gtttcttagc gcaggatgat accgtaaaag aatttaatct tcttgttaaa
    10621 caaactaaaa atgttcgttt tccaatagtc aaccaagcta atgttgtcgt tggtgttgtt
    10681 agtgttcaag atattcttgg aaaagataaa gaagtaaaat tggcaacagt catgtcaaaa
    10741 aatatcattg ttgctaaacc tcgaatgagt ctagctaata tcagtcaaaa aatgattttt
    10801 gaagatttga atatgatgcc agtagtatcg gatgactttg aattattagg cgttattaca
    10861 agacgacaag ctgtagaaaa tttatcgatg tcgcaaggga cggatttata cacctatagt
    10921 gatcaaatct tgtctaattt acaaattgaa gatggtcact tttcgttttt agtagaaccc
    10981 gctatgattg accatacagg tagcttaaca caaggagttt tgacggagtt cttaaaagaa
    11041 atttgtattc gtgtcttaac tcgtaagcat caacgcagta ttgttgttaa acaaatgaca
    11101 ctttatttct tgcaacctgt tcaaattgat gaaataataa tggtaacgcc aacaattatt
    11161 tcagaaaaac ggcgcgaagc tacactcgat ttagaattaa aattagaaaa taaaattatt
    11221 gctaaagcta tgatagcggt aaaaattaat tagatataag gagaataaat agaaaatgat
    11281 tacattaaag tcagcacgcg aaattgaagc aatggataga gcaggtgatt ttttagccag
    11341 catccacatt ggacttcgtg atattatcaa accaggagtt gatatgtggg aagttgaaga
    11401 atacgttcgt agacgttgta aagaagaaaa cgtattgcca ttgcaaatcg gggttgatgg
    11461 tgcagttatg gattacccat atgctacttg ttgtgggctt aatgatgaag tggctcatgc
    11521 ctttcctcgt cattataccc taaaacaagg tgacctttta aaagtagata tggttttaag
    11581 cgaaccttta gataaatcaa ttgttgacgt ttcatcgtta aactttgaca acgtggctca
    11641 aatgaaaaag tacacggaaa catatagtgg tggtttggca gactcgtgtt gggcttatgc
    11701 tgtaggtgaa gtttcacaag aggtgaaaga cttaatgtct gtgacaagag aagcaatgta
    11761 tattgggatt gaaaaagcag ttatcggaaa ccgcattggt gatattggag ctgctattca
    11821 ggattatgca gagagtcgtg gttatggagt tgttcgtgat ttggttggtc acggtgttgg
    11881 tccaacaatg catgaagagc ctatggtgcc taactatggt acagcaggtc gaggtctccg
    11941 tcttcgtgag gggatggttt taaccatcga accaatgatt aatactggta cttgggaaat
    12001 tgatacagac atgaaaactg gttgggcaca taaaacctta gatggtggtc tttcttgtca
    12061 atatgaacat caatttgtta tcactaaaga tggtcctgtt attttaacga gtcaaggaga
    12121 agaaagaact tactaaaatg aaattaaaaa aattctttga ggatttgctg gctaaactag
    12181 aatatagacc cattcaagtt tttatgcgtc attttcaaag tgcagagatg gacttatcag
    12241 ccatagcagt tgcttattat ctattagtga cagcttttcc attactagtt attgctgcta
    12301 atatttttcc ttattttcat attaatgtgt cggatttatt gtcacttatg caaaaaaatc
    12361 ttccgaaaaa tatttatgaa cctgcctcac gcttagcagt agatgctttt tcgaaaccgt
    12421 ctaccggcat tttgggattc gcgtcattaa cagctttttg gacaatgtct aaaagtttga
    12481 cctctcttca aaaagccatt aataaagcat acggtgttga ccaacacaga gactttgtga
    12541 ttagtcgttt agtgggtgtg gggactgggc tgatcatcct atttttactg acttttgttt
    12601 taattttttc aaccttttca aaaccagttt tacagataat tgtaaacatg tatgatttgg
    12661 gagatactct aacggcgtgg cttttaaatc ttgcacaacc agttacattt ttgactatat
    12721 ttttaggtat tggaatttta tactttattt tacctaacgc acgaattaga aaagtacgtt
    12781 atgtcattcc agggacatta ttttcaacgt ttgttattgg tttttttagt aatttaatta
    12841 gccaatacgt cttaaatagg gttgagaaaa tggtagatat taaaacgttt ggttcggtcg
    12901 ttatctttat cttaatgcta tggtttattt tcttagccca cattatgatt ttaggtgcaa
    12961 tactgaatgc cagcgttcaa gaaattgcga caggaaaaat tgaatcaagg cgtggagata
    13021 ttatgtctct tatccaaaaa tccaaagaag aaaaataaga aaagacagct cttgttagag
    13081 ttgtcttttt ttgtctttaa aaataacaaa tttgctcaag atgtagttga gtaaaataat
    13141 taatatttga gatacaaggc tttcaatggt gttgatttta tttaaattgt gctgaacgaa
    13201 ttgtccgata atagaaggaa attgatcaac aaaaataaca gctaatatca aatctaatcc
    13261 taatgtgcct aatctagcga tgacaaatgt ttgtaagcgt tgaagctgtc caattttagt
    13321 ttgtttaaaa acaaatcgat catttgtaaa aaaagcaaaa agtatagcta ttatgttagc
    13381 aactattgca gagaatggag cgtcctgcga gaaattaaaa atcccttgtc gaataactaa
    13441 ataaagaagg gatgttaata gtccgaacag taaatataag ctgacttcat tttgaatact
    13501 ttttttcagt aacgatttta atttaaccat atttttatta tatcacggat gcttcaaagt
    13561 gatgaggaaa tctcccataa gcttggcttt actttttgtt tgtgatatac ttagtaagtt
    13621 gttttacaca acccttgatg cttagttccc tttcatcaag catattacaa acgttagaag
    13681 tgttaactat ctaaacgtta aaggagaaat tatgaacaca tttacaactc gcgattacgc
    13741 tcacatggct attgtaacag ctatctatat cgttttaacc ataacgccac cttttaatgc
    13801 tattgcttat ggtgcttatc aattccgtgt ttcagaaatg ctaaatttct tggcctttta
    13861 ccaccgaaaa tacctttttg cggtaacgtt aggatgtatg atttctaatc tttatagttt
    13921 tggaatgatt gatgtctttg taggtggtgg ttcaacctta ctttttgttt atcttggaac
    13981 cattttattc aagcaatatc aaaaagatta tctatttaat ggccttatta acaaagcatt
    14041 ctttttcttt tctttcttct ttgctgcttc aatgattacg gtagcagttg aattgaaaat
    14101 tgtagcagga ttacctttat tattaacatg gttaacgaca gctgttggtg aattggcttc
    14161 acttttggtt ggagcagtcc ttgtggataa gttgagtaga cacgtcgatt ttacaaaata
    14221 aatatattat aaagcgcgag acattgatct cggctttttt gtttgggttt atagttagta
    14281 tagctagaaa atgctgcaac ttttatgaga aatgctccaa tttttactaa tatccttagt
    14341 ctttgtgata aaatagaaat atggaaaatc gtatgaatga attagtatct ttgttgaatc
    14401 agtacgcaaa agagtattat acacaagata atcccacagt ttctgacagt caatatgacc
    14461 agttatatcg agagttagta gaattagaaa agcaacatcc agaaaacatt ttgccaaata
    14521 gtcccacaca tcgagtgggt ggtttagttt tagaaggctt tgaaaaatac caacatgaat
    14581 atcctttgta ttcattgcag gatgctttct caaaagaaga attgatagcc tttgataaac
    14641 gtgttaaagc agagtttcca actgctgctt atatggcaga gctgaaaatt gatggtctat
    14701 ctgtctcgtt gacttatgta aatggtgttt tacaggttgg ggcaacacgt ggtgatggca
    14761 atattggaga aaatattact gaaaatttaa agcgtgttca tgatattcct ctacatttag
    14821 accaaagttt agatattact gttcgcggag agtgctacct tcccaaagaa tcttttgaag
    14881 ctattaacat agaaaaacga gcaaatggtg agcaggaatt tgctaatcct aggaatgcag
    14941 cagcaggaac cttgcgccaa ttaaacacag gaattgttgc gaaacgtaaa ttagcaactt
    15001 ttctttatca agaagctagc cctacgcaaa aagaaacaca agatgatgtt ttaaaagagc
    15061 tagaaagcta tggcttttca gtcaatcatc atcgtttaat tagctcgtct atggagaaaa
    15121 tttgggattt tatccaaact atagaaaaag atagagtttc cctcccttat gatatcgatg
    15181 gtattgttat taaggttaat agcttagcaa tgcaagaaga gctgggcttc actgtaaagg
    15241 cacctcgttg ggcgattgca tataaatttc ctgctgaaga aaaggaagca gaaatattat
    15301 ctgttgattg gacagttggg cgaacaggag tcgtaacacc aacagctaac ctaactccag
    15361 ttcaactagc agggacaacc gttagtaggg ccactctgca taatgtagat tatattgccg
    15421 aaaaagatat ccgtattgga gatactgttg ttgtatacaa agcaggggat atcatccctg
    15481 cagtcttaaa tgtcgttatg agtaagcgaa accagcaaga agtcatgctt attcctaaac
    15541 tatgtccttc ttgtggtagt gaattagttc attttgaagg tgaggtggct ttacgttgta
    15601 ttaatccgct gtgccccaac caaatcaaag aacgtttagc tcattttgct agccgagatg
    15661 ctatgaatat tacaggtttt ggtccatcct tagtcgaaaa attatttgat gctcatttaa
    15721 tagcagatgt tgcagatatt tatcgtttaa gtatagagaa tttattaact ttagatggta
    15781 ttaaagagaa atctgcaact aagatttatc atgcaatcca atcttcaaaa gaaaattctg
    15841 ctgaaaaact tctttttggc ctaggtattc gtcatgtggg aagcaaggcg agccgccttt
    15901 tattggagga atttggtaat ttgaggcaac taagtcaagc aagtcaagaa agtattgcct
    15961 caattgatgg actaggaggg gttattgcca aatcacttca taccttcttt gaaaaagaag
    16021 aggtagataa actactagag gaattaactt cctataacgt taattttaat tatctaggaa
    16081 aacgagttag tacagatgca caactttcag gtttaacagt cgttttaacc ggtaagcttg
    16141 aaaaaatgac tcgtaatgaa gcaaaagaga aattacaaaa ccttggtgct aaggtgacag
    16201 gtagcgtttc taaaaagact gatttaattg ttgcaggaag cgatgcagga agtaaactaa
    16261 ccaaggctca agatttaggg attacaatac aagatgaaga ctggttactc aacctttaag
    16321 tgaggaagaa atgaagaaac aaaaaaaagc gcgtttaatt tataacccaa cttctggtca
    16381 agaaattatg aaaaaaaatg tagcggaagt tcttgatata cttgaaggat ttgggtatga
    16441 aacatcagcc tttcaaacaa caccaactaa aaattctgcc agagatgaag ctactagagc
    16501 tgctcaagct ggttttgatt tgattgttgc agcaggtgga gatggtacta ttaatgaagt
    16561 tgttaatggt attgcacctt tgaaacgtcg tcctaagatg gcaattattc caactggaac
    16621 aactaatgat tttgctcgtg ccttaaaaat accgcgaggt aatcctattg aagcaactaa
    16681 attaattggt aaaaatcaaa tcgttaagat ggacattggt caagctcaag aggacaatta
    16741 ttttattaat attgcagctg caggtagttt gacagagtta acttatagtg taccaagtca
    16801 attgaaaaca acgtttggct atcttgctta tctagcaaag ggtgttgaat tattacctcg
    16861 agttcgaaaa gtaccagtca aaattactca cgataagggt gaatttatcg gtgatgcttc
    16921 aatgattttt gttgccatca caaattctgt aggagggttt gaacaaattg cacctgatgc
    16981 caaattggat gatgggaaat ttacccttat tttagtaaaa actgcaaatc ttattgagat
    17041 tatgcatctc attcgtttgg tgctagcagg tggaaaacat attaatgata aacgagtaga
    17101 gtatattaaa acgtcttatc taactatcga acccctatca gacgagcgta tgatgattaa
    17161 cttagatgga gaatatggag gagatgctcc cattactcta gcaaacctta aaaatcatat
    17221 taggttcttt gctaatactg atgaaatttc agatgatgct ttagttttag ataaagatga
    17281 actagcgata gaagctattg cacagaagtt tgctaatgag gtagatgatt tagaaggatt
    17341 aggagattga tgaatggata atactgtcat tgtacattac catagccgaa agaaaaatta
    17401 ctttaattta agtttatggc agtggcgcga tggctcagag ggtcaagatg ctcatttttc
    17461 acggtttgac agtttcggtg ctgttgcgat actaaaatat cccttgccat attttttaag
    17521 ccattattat gtgattgtta aggataaaaa ttggcatcat aaaacaatag attatcgcat
    17581 tgattgtact caaggtggtg cacgtaaaga agtttggatt gtagatgggg atgacaatct
    17641 gtattattct cgtcaagcag cagttagtag ccatcattat agtagacgcc aacctcatgc
    17701 ctacgacatg gcaatcaata gtagagcgtt tgacaaaaaa tggggatttg atggttggtt
    17761 aggttttgaa tattcagaaa ctaaaacggc ttttagatta tgggccccta ctgcggaacg
    17821 tgtagaactg attttatatc attcgacaga tgaaacagca agtgtatcaa aagttctttc
    17881 tatgaaaaga gggacagccg tcaattataa aaatcataaa gaaaataccc atggagtctg
    17941 gtttactgaa cttgaaggaa actataatta ccaagcatat acttatcgag tctactatag
    18001 acgacgtacc tttaaaatta cgagagaccc ttattccatt gcaacgactg caaatggtaa
    18061 aaggtctatt gttattgctc cagaagcatt aactccggaa gggtttaaaa taagtcatgg
    18121 aaaagaagct aagtggcggc tcgaaaaccc caatcaagca gtcatttacg aaatgcatgt
    18181 gcgtgatttt tcgatatctg agacttcagg agttaagaca gattatcacg gaaaatttaa
    18241 gggattgcat caaaaaggta cggttaatca gcatggtgat aaaacgacct ttgattatgt
    18301 acaagaccta ggagttaact atatccaact tcaaccaatt ttcgatcatc atcagacatt
    18361 tgatgatgat ggtcactatg cttataattg gggatatgat cctgaaaact acaatgttcc
    18421 agaagcaagc ttctctagta atccacatga acctgcaacg cgtattttag aattaaaatc
    18481 tgctatccaa gcgtaccatg atgcaggcat tggtgttatt atggatgttg tctataatca
    18541 tactttttca tcaactgatt ctgcatttca actaacggtt ccagattatt attatcgaat
    18601 gaatcacaat ggaacatttc aaaatgggtc aggctgtggt aatgaaacag ctagtgaaaa
    18661 agaaatgtgt cgtaagtata ttcttgattc agttttgtat tgggtgaaag aatataatat
    18721 tgatggtttt cgatttgatt tgatgggact acatgatgta gaaacgatga acattataag
    18781 aaatgagcta aataaaatcg accctcgtat tttggtttat ggagaaggat gggacatggg
    18841 agctggtctc acgcctcaaa ataaagctaa aaaagataat gcttatcaga tgcctggaat
    18901 tggctttttc aatgatgatg taagagacgc tgtcaaaggt gcggaaattt acggagaatt
    18961 taaaaaaggc ttagtttctg gaaacagtac agaagatatt gttgcaaaag gcattttagg
    19021 aagtgatgag ttagtatcat acattgatcc tagtcaagtt ttaaattatg ttgaagcaca
    19081 tgacaactac aacttaaatg atttgctatg ggaattacat cctaatgaca acgagaaaca
    19141 acatatttac cgagttgaag tagctagtgc tatgaatcta ttgatgcaag ggatggcttt
    19201 catgcaatta ggtcaggagt ttcttcgaac aaagtgttat cctacaggtg ataaaggtca
    19261 acttactcaa gcagataaag aaagggctat gaattcttac aatgccccag accaagttaa
    19321 tcaggtcaac tgggataatg ttacctttca taagagtact attaatttta ttaggaaaat
    19381 aattactttg aagacaaact ctccttactt ttcttattct agctttgaag agattcgcaa
    19441 acatgttttt gtagagtctg ctcaatacca ttcaggtttc ataagtttta cagtcgaaga
    19501 acataagaaa ataaaaataa ttttcaatat taaaggaaaa cgcttgcaga attctttcat
    19561 aggtgatata ataatatcaa atcatgaaga aggaaaagcg atagattcat tagatgatga
    19621 gctttctgta atgatccttg atatcacaaa ataacctgaa aacattcagg ttatttttgt
    19681 gtatattaag gaatttgatt tgaatttgag tttcttatat tttagttaat ttttcggaaa
    19741 tatagagggt gaaatatttt cagatgcgtc tgtatgagga aaatgtattc ctcttttttt
    19801 gtaccattga ggaaatattt ttgtcttata ttgactagta ggacctgtga ggtgagtaga
    19861 tgtccaataa agatgagtta tatacttttg gaataggtga aaattttcat ctccagaatt
    19921 atttaggcgt acatagcgaa aatggaagct tttgtttcag agtttgggct cctaatgctg
    19981 aaaatgttca agttattgga gattttacag attggcgaaa tcgacctctc caaatgaaca
    20041 aaaatcaagc aggagtttgg gaagctaaca gtttggatgc gcgcgaaggt gatttatata
    20101 aatatttggt tactcgtaaa ggtggtcaag tagttgagaa aatcgatcct atggcagttt
    20161 atatggaaag acgtcctgga actgcaagtg ttattaaagt tcttagaaat aaaaagtggg
    20221 aagatggtct ttggatggga aggcgcaaac gtctgggttt tcagaagcgt ccaattaata
    20281 tttacgaagt tcatgctggt tcatggaaaa aagatgattt tggacatcct atgacttttt
    20341 ctcagttaaa agattactta atcccctacc ttgttgagat gaattatact catgttgaat
    20401 ttatgccctt aatggcacat ccgcttgata tgagttgggg atatcaatta atggggtatt
    20461 ttgcttttga acatacctat ggtactccag aagaatttca agactttgta gaagcatgtc
    20521 ataaaaataa tataggagtt cttgtagatt gggttccagg acactttatt cagaatgacg
    20581 atgctttagc ctatttcgat ggaactgcta cttatgagta tcaaaatcat gatcgtgcgc
    20641 ataactatcg atggggagct ctgaactttg atttaggaaa aaatcaagta caatcctttt
    20701 tgatttcgag tgccttgttc tggattgaac attatcatat tgatggtatt cgtgttgatg
    20761 cggttagtaa tatgttatat ctcgattatg atgaaggacc atgggaagct aaccaatttg
    20821 gggataatcg taatttggaa ggatatcact ttttaaggaa acttaacaaa gtaattaaag
    20881 agcgtcatcc taatgttatg atgattgctg aagagtcaac agcttctacc ccaataacta
    20941 aagacttaga gtctggagga ttgggttttg actttaaatg gaatatgggc tggatgaatg
    21001 atatcctgcg tttttatgaa gaagatcctc tctataggca atatgatttt aatttagtga
    21061 cttttagttt tatgtatata ttcaatgaga actttgtact agctttttca catgatgaag
    21121 tagtacatgg taaaaagagt atgatgcata aaatgtgggg tgaccgttat aatcaatttg
    21181 ctggtttacg taatttatat gcatatcaaa tgtgtcatcc tgggaaaaaa cttctcttta
    21241 tgggaagtga gtttggtcaa tttttagaat ggaaatataa tgatcaacta gaatgggaaa
    21301 acttaaatga tgatatgaac caaaagatgc aacgctatac taaacaattg aatcaatttt
    21361 ataaagatca taagtgccta tggcgtattg atgattcctt tgacgggcta gaaattattg
    21421 atgcggataa taaatctgaa actgttttat cttttatacg aaaagatgat aaaggcgatt
    21481 tgttactttg tgtctttaat atgactcctg tagaacgccc taactttact attggtgttc
    21541 cgcaagcggg tatttatgaa gaagttttaa atactgaaat ggaagagttt ggaggtgttt
    21601 ggaaaaacca caatcctgta acgaaaacac aagtggctac atggaaagat tatgatcata
    21661 ctttaagttt tacattacca gcccttgggg caagtgtttg gagaataaaa cgtcggcttc
    21721 gtaaataagt ctgcaattta gtaacctcat tttgaaagga aagaataata tgaaaaatga
    21781 aatgttagca ctcattctcg ccggaggaca aggcactcga ctcggaaaat tgactcagag
    21841 cattgcgaaa cctgctgttc aatttggagg tcgttaccgt attattgact ttgcgctttc
    21901 aaattgtgcg aattcaggta ttaataatgt tggagttatt actcagtatc aaccgctgga
    21961 actcaatact catattggaa acggatctag ctggggacta gatggtatcg attcgggagt
    22021 aactgtatta cagccatatt ctgcaacaga aggtaatcgc tggtttcaag gtaccagcca
    22081 cgctatctat caaaatattg actacattga tcgtattaat ccagaatatg tactcattct
    22141 ctctggggac cacatctata aaatgaacta tgatgacatg ctacagacgc ataaagataa
    22201 tttagcaagc ctaacagtcg ctgttttaga tgttccttta aaagaagcaa gccgttttgg
    22261 aattatgaat actgattcaa acgaccgtat tgtcgaattt gaagaaaaac ctgaacatcc
    22321 taagtcaaca aaggcttcaa tgggaatata catttttgac tggaagagat tacggacagt
    22381 tttaattgat ggtgaaaaga acggcataga catgtcagac tttggtaaaa atgttatacc
    22441 agcatacctt gaatcgggtg aacgtgttta tacctataat tttgatgggt attggaaaga
    22501 tgttggtact attgaatccc tttgggaagc taatatggaa tatattggag aagataacaa
    22561 attacatagt cgtgatcgtt catggaaaat ctattctaaa aatttgattg caccacctaa
    22621 tttcatgaca gaagatgcaa atgttaaaga ttctttagtt gtagatggat gctttgttgc
    22681 aggaaatgtt gagcactcca ttttatcaac taatgtccag gtaaaaccaa atgctattat
    22741 taaagactcc tttgttatga gtggcgcgac tatcggtgaa ggtgcaaaga taaatcgagc
    22801 tattatcggt gaggatgctg ttattggtga tggtgttgtg attgatggaa gtaaagaagt
    22861 agaagttatt ggttataaag aagtagcggg ggttccaaat gaagattgat aaatatactg
    22921 cgattttagg aaatactgtt agttacccag atatgcacag tttgacggat aatcgtccgc
    22981 ttgcttctct accttttgat ggtaagtatc gtttaattga ttttcaatta tcctctttag
    23041 ctaatgcagg cgttagaagt atttatggta tttttcgtgg gaataatgtt cgctctgttt
    23101 ttgaccatat tagaagtggt cgtgagtggg gcttaggtac actgttgagt cactattctt
    23161 taggattcta tgatacagac gatgatactc gtacagtcaa tggggattat tacgatcaaa
    23221 ttttaactta cttgaggcgt tcgggtagcg accagacgat atatatgagt tgtgatatgc
    23281 tttgtaatat tgacttaagt caagtaatcc acttgcataa tgcaaatggt gcgcaagcca
    23341 ccgtggttta taaaaaaatg ccgagatcag ctattgcaga ggctaatcaa gtcttgacca
    23401 ttgatgaatc tgatcatgta gtgagtctag gtaaagctgt agataatgac gataaccaaa
    23461 aaatgtctgc tgagatttat gttatcgata caccttggtt aatcgaaaaa atggaagagg
    23521 aagctcaaaa taatgaacca cgtaaactcc gcttcttact tcgtgattta attgttgaaa
    23581 gtaatgcttt ggcttttgaa tacactggtt atttgtcaaa catttcttct attaaatcct
    23641 attatgatgc taacatggac atgttaacac caaataaatt ttattcgctc ttcttttcaa
    23701 accaaaaagt ttatactaag gtgaaaaatg aggaagctac ttattttgat aaacaatcaa
    23761 atgtctctaa ttcgcaacta gcctctggaa gtattattaa aggttattta gaccattcta
    23821 tcgtttctag aaattgtctc cttgaaaaag ggactcgagt agtcaatagt attatctttc
    23881 caaaagtaaa aatcggagaa ggggcaacca ttgaaaatac tattattgac aaatgcgtta
    23941 aggttgcatc cggagttaca ttgaaaggta gtttagataa accgttagtg attcctaaat
    24001 tttcagagat taatgaggat attatacaat gaaaataatg tttgttgcgg cagagggtgc
    24061 tccctttgct aaaacaggtg gtttgggaga tgtgattggc gcactcccaa aatcacttag
    24121 taaaaaaggt catgatgtcg ctgttgtcat gccatattat gatatggtag accaaaaatt
    24181 tggagaccaa atagaaaacc tcatgtattt ctatacagat gttggctggc gtcatcaata
    24241 tgtaggagtt aagagacttt ctcaagataa tgttacgttc tactttattg ataatcaata
    24301 ttatttttat agaggtcatg tctatggtga ttgggatgat ggggagcgtt ttgcttattt
    24361 ccaattagct gctttagagt tgatggagaa aatcgatttt attcccgatg tcttacacgt
    24421 ccatgattat catacagcaa tgattccttt cttattgaaa gagaagtacc actggattca
    24481 ggcctataat aatataagag cagtttttac tattcacaat attgaatttc aagggcagtt
    24541 tggtccagaa atgttaggag atttatttgg agttggcgct gagagatatg aagatggaac
    24601 tcttagatgg aataattgtc ttaattggat gaaagcagca attctttact cagatagagt
    24661 gacaacggta tcaccaagct acgcaaatga aataaaaact ccagaatttg gtaaaggttt
    24721 agatcaaatt atgcgtatgg aagcaggcaa attatctgga attgtaaatg gtattgatag
    24781 cgatttgtta aatccagaaa cagatgcttt tttgccatat catttttcaa aaagcaactt
    24841 agagggcaaa attaaaaata agttggcttt acaggaaaat ttgggcttac ctcaagataa
    24901 aaatgtacca ttaattggta ttgtttcacg acttaccgat caaaaaggat ttgatattat
    24961 tgcttctgaa ttggacaata tgcttcaaca agatattcaa atggttattt taggaacagg
    25021 ctatcaccat tttgaagaaa cgttctctta ttttgctagt cgttaccctg aaaaactatc
    25081 tgctaatatt acttttgact taagattagc gcaacagatt tatgcggctt cagatatctt
    25141 catgatgcca agtgcttttg aaccttgtgg tctctcgcag atgatggcta tgagatatgg
    25201 tagcttgccc ttggtacatg aagttggagg attaaaagat actgtggtag ccttcaatca
    25261 atttgatggt agtggtacag gtttttcatt taatcatttt tcaggctatt ggttaatgca
    25321 aactctaaaa ctagcattag aagtttataa tgattatcct gaagcgtgga aaaagttaca
    25381 atggcaagct atgtcaaaag atttttcatg ggatacagca tgtgttgctt acgaacaact
    25441 gtatcagcaa ctacaataat caccctattt tagtgattag ttgataagct tatcgaatct
    25501 ctatgctaaa cgtttcttat gtaaaaccac tttagtatag agattttatt atcctataag
    25561 gtaaaatatt attaatgtaa agagacacta tatcaaaaat aaagtgttgt catatcttaa
    25621 taagggatta aattaaagct aaaaaaacta aaaattgtta tttgaaaagg attgtgtaaa
    25681 ccatctcaaa ctatttcatg aaaacgtaat caaatgagga aattcattga catgcacaag
    25741 aaataggaat aaaataacgg agtaaatatt ttttaaagga gaattctatt atgaatttag
    25801 ctattttagc acttggtttc gccgtgatgg gtgtttcaat cggtgaagga attttggttg
    25861 caaatattgc caaatcagcg gctcgtcagc ctgaaatgtt tagcaaactg caaacattga
    25921 tgtttacagg tgtggccttc attgaaggta ccttcttcgt tctatttgcg tttacattct
    25981 tagtaagata gacgacaatt tagaaaaaaa ggagggatga ggattggaat ctacaagtaa
    26041 tccaaccgta tcttttttag gcattgattt tgatttaacc atcctagcta tgtctctctt
    26101 gacaattact attatcttta tcttagtctt ctgggctagt cgtaagatga caataaaacc
    26161 aaaaggtaag cagaatgtct tagaatatgt ctatgagctt gttaataata caatctcaca
    26221 aaatttagga cactatacta aaaattatag tttattgatg tttattttat tcagttttgt
    26281 cttcattgct aacaacttag gattgatgac atcattgaaa acacatgagc ataatttttg
    26341 gacatctccg accgctaatt ttggtgtaga tattacatta tcattattag tagcatttat
    26401 ttgtcatatt gaaggtattc gcaaaaaagg aattggtggt tatctcaaag gtttcttatc
    26461 accaacacca gcaatgcttc cgatgaattt attggaagaa gttactaatg ttgcttcatt
    26521 agcattgcgt ctttttggta atatcttttc tggtgaagtt gtcactggat tacttttaca
    26581 acttgcagtt ttatcaccat ttacaggtcc actagctttt gcattgaata tcgtttggac
    26641 agcattctca atgtttattg gtttcatcca agcgtatgtg ttcattatct tgagctcaag
    26701 ttatattgga cacaaagttc atggtgacga agaagaataa gaaaagagag gagaaatatg
    26761 tcaatactta ttaatagtac aacgattggt gatatcatta tcgtatctgg ctctgtgctt
    26821 ctcttattta tcttaattaa gacttttgca tggaaacaaa tcacaggtat ttttgaagcc
    26881 cgtgaacaaa agatagctaa tgatattgat actgctgaac aggcacgtca acaagctgaa
    26941 gcttttgcaa ctaagcgcga ggaagaacta tctaacgcta aaactgaagc taatcaaatc
    27001 attgataacg ctaaagagac aggtcttgca aaaggtgatc aaattatttc agaagcaaaa
    27061 actgaagctg atcgattaaa agaaaaagca catcaagata ttgcccaaaa taaggcagaa
    27121 gcactagcag atgttaaggg tgaagtagca gatttaacag ttttattagc tgaaaaaatt
    27181 atggtatcaa atcttgataa agaagctcag agtaatctca ttgatagcta tattaaaaag
    27241 ctaggagatg cctaatgaat aaaaagacgc aggctctaat tgagcaatat tcaaaaagcc
    27301 ttgttgaagt tgccattgaa cataaaattg ttgaaaaaat ccaacaagaa gtagcagctt
    27361 taattgatat ttttgaaaca agcgagcttg agggtgtcct gtcaagtctt gccgtttcac
    27421 atgatgaaaa acaacatttt gtgaagacat tgcaaacatc atgttcaact tatcttgtta
    27481 atttccttga ggttattgta caaaatgaac gtgaagcact tctatatcct attttgaaat
    27541 caattgatca ggaacttatc aaagtgaatg gtcaatatcc aattcaaatt acaactgcag
    27601 ttgctttgtc tccagaacaa aaagaacgtc tttttgatat tgctaaaact aaattagctt
    27661 taccaaatgg acagttagtt gagcacattg acccatctat tgttggtgga tttgttgtca
    27721 atgcaaacaa taaagtaatt gatgcaagtg ttcgtaatca attacatcaa tttaaaatga
    27781 aattaaaata gaaagtggtg tgacttttgg caattaatgc acaagaaatt agcgctttaa
    27841 ttaaaaagca aattgaagac ttccagccaa attttgacgt cacagaaact ggtattgtta
    27901 catatatcgg tgatggtatt gctcgtgctc gtgggctaga taatgctatg agtggagaac
    27961 ttcttgaatt ttcaaatggt gcgtacggta tggctcaaaa ccttgagtct aatgacgtgg
    28021 gtatcattat tttgggtgat ttctcagaaa ttcgtgaagg tgatgtggta aaacgtactg
    28081 gtaaaattat ggaagtacca gtaggggaag ctatgattgg acgtgtcgtc aatccgcttg
    28141 gtcaaccagt tgatggacta ggtgaaattg agacgacagc aacacgccca gttgaaacac
    28201 cagcacctgg tgttatgcaa cgtaaatcag tttttgagcc tcttcaaaca ggtctcaaag
    28261 cgattgatgc tttggttcct atcggacgtg gtcaacgcga attgattatc ggtgaccgcc
    28321 aaactggtaa gacgtctgta gcgattgatg caatcttgaa tcaaaaaggt caagatatga
    28381 tctgtatcta tgtggctatc ggtcaaaaag agtctacagt acgtacacaa gttgaaactt
    28441 tgcgtaaata cggtgccctt gattatacaa tcgttgtgac agcttcagca tcacaaccat
    28501 ctccattact tttcattgct ccatatgcag gagtagcaat ggctgaggaa ttcatgtata
    28561 acgggaaaca cgttttgatt gtttatgatg atttatcaaa acaagccgta gcttatcgtg
    28621 aactttcgct tcttcttcgc cgtcctccag gtcgcgaagc ttacccaggg gatgttttct
    28681 accttcatag tcgtcttttg gaacgttcag ctaaagtatc agatgcttta ggtggaggtt
    28741 cgattacagc gcttccgttc attgaaacac aagcaggtga tatctcagca tatattgcta
    28801 caaacgttat ttcaattact gacggccaaa ttttcttgca agaaaatctc tttaactcag
    28861 gtattcgtcc agcgattgat gcaggttcat cagtatctcg tgttggtggt gcagcgcaaa
    28921 ttaaagcgat gaagagagtt gcaggtacgc ttcgtctaga tcttgcttct tatcgcgaac
    28981 ttgaggcatt cactcaattt ggttcagacc ttgatgcagc aacacaagca aaactaaatc
    29041 gtggtcgtcg cacagtggaa gtgttaaaac aaccattaca caaaccatta ccagttgaga
    29101 aacaagttgt gattttatat gctctaacac atggattcct tgatgatgta cctgtaaatg
    29161 atattttagc ttttgaagaa gctttgtatg attactttga tgctcattat gataatctct
    29221 tcgaaacaat ccgcacaaca aaagatttac cagaagaagc agaattagat gctgctattc
    29281 aagcatttaa agatcaatca caatttaaat aataggaggg ttctaatatg gcaggctctc
    29341 ttagtgaaat taaagacaag attctctcta cagaaaaaac aagtaagatt actagtgcga
    29401 tgcagatggt ctcatctgct aagttggtta aatctgaaca agctgctaga gattttcaag
    29461 tgtatgcctc taaaattcgt caaattacta ctaacctctt aaagtcagac ttagtatctg
    29521 gatcagataa ccctatgtta tcttcacgtc cggtgaagaa aacaggttat attgttatta
    29581 cttctgataa gggtttagtt ggtggttaca attcaaaaat cttaaaagcg atgatggaca
    29641 ctataactga ctatcatact gagaatgatg attatgccat catttcaatt ggtagtgttg
    29701 gttctgattt tttcaaagca cgtggaatga atgtttcttt cgaattacgt ggtttagagg
    29761 accaaccaag ttttgatcaa gttggtaaaa ttattgctca agcagttgaa atgtacaaaa
    29821 atgagttgtt cgatgaatta tatgtttgct acaatcacca tgttaacagt ttaattagcc
    29881 aagtcagaat gcaacaaatg cttcctatca aagaattgga tgcagaagag gcttctgagg
    29941 atagagtgat tactggtttt gaacttgagc caaaccgtga agttatattg gagcaattat
    30001 tgcctcaata tactgagagt ttgatttatg gagctattat tgatgctaaa acagcagagc
    30061 atgcggctgg tatgacagct atgcagactg caactgataa tgctaaaaat gttatcaatg
    30121 atttaactat tcaatataac cgcgctcgtc aagcagctat cacacaggaa attacagaaa
    30181 ttgttgcagg tgctaatgca cttgaataat taattagtct atagaaaaaa tagtgaacaa
    30241 aggtatttta aatcctttgt atcatatgat aaggagaaat acatgagctc aggcaaaatt
    30301 gctcaggtag ttggacctgt cgttgacgtt gtatttgcaa gtggcgacaa acttcctgag
    30361 attaataatg cattgattgt ctataaaaat ggcgataagt cacaaaaagt agtacttgaa
    30421 gttgctcttg aacttggtga cggcctcgtt cgtacaatcg ctatggaatc aactgatggg
    30481 cttacacgtg gtttggaagt attagatact ggtcgcgcaa ttagtgtgcc ggttggtaag
    30541 gatactttgg gtcgtgtctt caacgttctt ggagatgcta ttgaccttga agagcctttt
    30601 gcagaagatg cagaacgtca accaatccat aaaaaagcac catcgtttga tgaattatca
    30661 acatcatcag aaatcttaga aactggtatt aaagttattg acttattagc accttactta
    30721 aaaggtggta aagttggact tttcggtggt gcaggtgttg gtaaaaccgt tcttattcaa
    30781 gaattaatcc acaacatcgc ccaagaacat ggtggtattt cagtatttac tggtgtagga
    30841 gaacgtactc gtgaagggaa tgacctttat tgggaaatga aagaatctgg cgttattgaa
    30901 aaaacggcta tggtctttgg tcaaatgaat gaaccaccag gagcacgtat gcgtgtggca
    30961 cttactggtc ttacaatagc tgagtacttc cgtgatgtag aaggacaaga tgtgcttctc
    31021 ttcattgata atatcttccg tttcacacaa gctgggtcag aagtgtcagc gcttttaggt
    31081 cgtatgcctt cagccgttgg ttatcaacca acacttgcta cagaaatggg acaattacaa
    31141 gagcgtatca cttcaactaa aaaaggttct gttacatcaa tccaagctat ttatgtgcca
    31201 gcagatgact atactgaccc tgcgccagct acagcctttg ctcacttaga ttcaacaact
    31261 aaccttgaac gtaaattgac acaaatgggg atttatccag ccgttgaccc attagcttca
    31321 agttctcgtg cactcacacc agaaatcgtt ggagatgagc actatgaagt tgcaacagaa
    31381 gtacagcgtg ttcttcaacg ttaccgtgag ttacaagata tcattgcgat tttaggtatg
    31441 gatgaattat cagatgagga aaaaacgtta gttggacgtg cacgtcgtat ccaattcttc
    31501 ctttctcaaa acttcaatgt tgcagaaaca tttactggtc aaccaggttc atatgttccg
    31561 gttgaagaaa ctgttcgcgg attcaaagaa atcctagatg gtaaacatga tcaaattcca
    31621 gaagatgcct tccgtatggt tggtggtatt gaggatgtta tcgcaaaagc agaaaaaatg
    31681 aactattaat gaggtgatgc tatggcacaa ttgactgttc aagtagtaac accagatggt
    31741 attagatatg accatcacgc tagtctcatt actgttagaa cgccagatgg tgaaatggga
    31801 attttaccag gtcacataaa tttgattgct ccccttaatg ttcatcagat gaagattaat
    31861 cgttctcatc aagaaggtgt agactgggta gctgttaatg gtggtataat tgaagttaat
    31921 gaggatcagg tgacaattgt tgcggattca gcagaacgag cacgtgatat agaccttaac
    31981 cgtgcagaac gggctaagga acgcgctgag cgtgcattag aaaaagctca aacaactcag
    32041 aatattgatg agatgcgtag agctgaggtt gctcttcgtc gcgcaattaa ccgtattagc
    32101 gtcggtaaaa agtagaaaaa tggtctaatt agttttgtga ctaattaggc ttttttagtg
    32161 tataatagaa ggcatgaata tgatattaaa tatagtaact atagttagta attttatttt
    32221 tattgccctt ttttatcaac tttttgtaga tttatttgac tggtctaaaa tgattaaaat
    32281 gtcccctcaa aacatctcaa aattaaaagt ttttattttg ttgataagta tagttggtgg
    32341 ctatgtggtt agtcattttt tattggaagt gattcagttg tgtcaatcaa tcttttgggc
    32401 tctccaataa aggaaatatt agctgaacta tgatataatc aacttactat tgttataaga
    32461 atggagattc aaatggataa aattattgtg gaaggtgggc aaacgcagct tcaaggtcaa
    32521 gttgttattg aaggagctaa aaatgcagtt ttacctcttt tagcagccac aattttacca
    32581 agccaaggga aaacattgtt gaccaatgtt cctatcttgt ctgatgtatt tacaatgaac
    32641 aatgttgttc gtggtttaga tattcaagtt gactttaatt gcgataaaaa agaaatctta
    32701 gtggatgcct caggtgatat tttagatgtt gcaccttatg aatttgttag tcaaatgaga
    32761 gcttctattg ttgttttggg acctatttta gctagaaatg gccatgctaa ggtttcaatg
    32821 ccaggcggtt gtacaattgg tagccgacca attgaccttc atcttaaagg tttagaagct
    32881 atgggagcta cgattacaca aaatggtggt gatattactg cccaagctga aaagcttaag
    32941 ggagctaata tatacatgga ttttccctca gtcggtgcca ctcaaaactt aatgatggct
    33001 gctacacttg cttcaggcac aacaacgata gagaatgctg cacgtgaacc agaaattgta
    33061 gaccttgcgc agttactaaa taaaatggga gctaaagtaa agggtgctgg tacggaaaca
    33121 ttaacgatca ttggtgttga tgctttacat ggtacagaac atgatgttgt ccaagatcgt
    33181 attgaagctg gaacctttat ggtggcagct gctatgactt ctggaaatgt tctagtaaag
    33241 gatgctattt gggaacataa ccgtccttta atctctaaat tgatggagat gggtgttgaa
    33301 gtgtcagagg aagaagatgg tattcgtgta aaagctgata ctaaaaaatt gaagccagtg
    33361 actgtgaaaa cactccctca tccaggattt cctactgata tgcaagctca atttacagct
    33421 ctaatggcag tagttaatgg cgaatcaact atgattgaga cagtttttga aaatcgtttt
    33481 caacatttgg aagaaatgcg tcgcatggga ttacaaacgg aaattttacg agatactgca
    33541 atgattcatg gtgggagagc tttacaaggt gcaccggtca tgtctactga cttacgagct
    33601 agtgcagctt taatcttggc aggaatggtt gcgcaagggc aaacagtagt tggccaatta
    33661 acccatttag accgtggtta ttatcaattc catgaaaaat tagcggctct tggtgctaat
    33721 attaaacgtg taagtgaggc gtaatatggc atctagtggt tggaaatatg tattaaaaca
    33781 aatcggtcta atagtattag tcatactatt agccttgctg tttttagcgg ttggattgat
    33841 gttgggttat agtgtttttg gtgacggaga gcatgcttac tctattttat cgctggataa
    33901 atggcaaaat attattggaa aatttttagg aaaatagaaa gagccactat aagttatttg
    33961 atgcttatag tggtttcctt tgagagttaa ttttagcagt agaataatac aatgacaaaa
    34021 aaacaaaaac aaataacagc ttcgattgta actttgcttt tttcattact ggcaataggg
    34081 ttaggtgaaa gcaaatcttt gcctaatgat cacattttaa aacaggtgac agatttaatt
    34141 attagtaatg aatctggcaa aaatacttcc cttaaaaatg tatcaggaac tcctagtaga
    34201 gagttatctg aatctgtttt gactagcaat gttaaaaaac aattgggaac aaatatagct
    34261 tggaaccagt caggtgcttt tatcattaat caaaataaaa cagatttgaa tgctaaagtg
    34321 tcaagtgcac catatgctat taatgaaata aagaaggtta ataaccaaat tgttccgact
    34381 aaagcaaatg ccttattaac aaaagcaact cgtcaatatc gaaatcgaga agaaacaggg
    34441 aatggtagaa cttattggaa accagctgga tggcatcaga taaatggatt aaaagggagc
    34501 tataaccacg ctgtggacag aggccattta attggttatg ctttagtagg gagtttaaga
    34561 ggatttgatg cctccacaag taatcctaaa aatattgcca cacaagcagc ttgggcaaac
    34621 caagctaata gcaaccaatc aacagggcaa aattattatg aaactttagt tcgtaaagca
    34681 ttggatcgtc ataaaactgt taggtatcgt gtgactttaa tttatgacag agataatctt
    34741 ttatcttcgg gatcacatat tgaggctaaa tcttcagatg gaagtttaga atttaacgtt
    34801 tttattccta atgtacaatc gggattatta tttgattatg caacaggaaa agtgaagcag
    34861 acaaagtaat ctttat
//
LOCUS       DATRSD010000009        33838 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRSD010000009 DATRSD010000000
VERSION     DATRSD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33838
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.009"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..1129)
                     /locus_tag="VBD85_000367"
     CDS             complement(<1..1129)
                     /locus_tag="VBD85_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7132942.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGEISELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTS"
     gene            complement(1216..1719)
                     /locus_tag="VBD85_000368"
     CDS             complement(1216..1719)
                     /locus_tag="VBD85_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7132943.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(1805..3136)
                     /locus_tag="VBD85_000369"
     CDS             complement(1805..3136)
                     /locus_tag="VBD85_000369"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO7132944.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(3146..3763)
                     /locus_tag="VBD85_000370"
     CDS             complement(3146..3763)
                     /locus_tag="VBD85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132945.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRDFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAHYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(3928..7026)
                     /locus_tag="VBD85_000371"
     CDS             complement(3928..7026)
                     /locus_tag="VBD85_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7132946.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(7181..8491)
                     /gene="der"
                     /locus_tag="VBD85_000372"
     CDS             complement(7181..8491)
                     /gene="der"
                     /locus_tag="VBD85_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO7132947.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(8539..9441)
                     /gene="dnaI"
                     /locus_tag="VBD85_000373"
     CDS             complement(8539..9441)
                     /gene="dnaI"
                     /locus_tag="VBD85_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO7132948.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMTRELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(9438..10613)
                     /locus_tag="VBD85_000374"
     CDS             complement(9438..10613)
                     /locus_tag="VBD85_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO7132949.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKVQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(10613..11092)
                     /gene="nrdR"
                     /locus_tag="VBD85_000375"
     CDS             complement(10613..11092)
                     /gene="nrdR"
                     /locus_tag="VBD85_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO7132950.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(11241..12746)
                     /locus_tag="VBD85_000376"
     CDS             complement(11241..12746)
                     /locus_tag="VBD85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO7132951.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(12736..13425)
                     /locus_tag="VBD85_000377"
     CDS             complement(12736..13425)
                     /locus_tag="VBD85_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7132952.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(13660..14193)
                     /locus_tag="VBD85_000378"
     CDS             complement(13660..14193)
                     /locus_tag="VBD85_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO7132953.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQDLV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(14298..15188)
                     /gene="htpX"
                     /locus_tag="VBD85_000379"
     CDS             complement(14298..15188)
                     /gene="htpX"
                     /locus_tag="VBD85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO7132954.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(15209..15763)
                     /locus_tag="VBD85_000380"
     CDS             complement(15209..15763)
                     /locus_tag="VBD85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO7132955.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            15891..16604
                     /gene="rsmG"
                     /locus_tag="VBD85_000381"
     CDS             15891..16604
                     /gene="rsmG"
                     /locus_tag="VBD85_000381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO7132956.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDRVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            16619..17998
                     /locus_tag="VBD85_000382"
     CDS             16619..17998
                     /locus_tag="VBD85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7132957.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQSDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            18011..18682
                     /locus_tag="VBD85_000383"
     CDS             18011..18682
                     /locus_tag="VBD85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO7132958.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINSPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(18717..19547)
                     /locus_tag="VBD85_000384"
     CDS             complement(18717..19547)
                     /locus_tag="VBD85_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7132959.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(19540..21216)
                     /locus_tag="VBD85_000385"
     CDS             complement(19540..21216)
                     /locus_tag="VBD85_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7132960.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVFYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(21233..21781)
                     /locus_tag="VBD85_000386"
     CDS             complement(21233..21781)
                     /locus_tag="VBD85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO7132961.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMTKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(21950..23158)
                     /gene="sstT"
                     /locus_tag="VBD85_000387"
     CDS             complement(21950..23158)
                     /gene="sstT"
                     /locus_tag="VBD85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO7132962.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            23334..24701
                     /gene="brnQ"
                     /locus_tag="VBD85_000388"
     CDS             23334..24701
                     /gene="brnQ"
                     /locus_tag="VBD85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7132963.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVAYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMALKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            24815..25870
                     /locus_tag="VBD85_000389"
     CDS             24815..25870
                     /locus_tag="VBD85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7132964.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(25919..26611)
                     /locus_tag="VBD85_000390"
     CDS             complement(25919..26611)
                     /locus_tag="VBD85_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7132965.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(26608..27678)
                     /locus_tag="VBD85_000391"
     CDS             complement(26608..27678)
                     /locus_tag="VBD85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO7132966.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(27671..29047)
                     /locus_tag="VBD85_000392"
     CDS             complement(27671..29047)
                     /locus_tag="VBD85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO7132967.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFIKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(29181..30005)
                     /locus_tag="VBD85_000393"
     CDS             complement(29181..30005)
                     /locus_tag="VBD85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7132968.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(30146..30979)
                     /locus_tag="VBD85_000394"
     CDS             complement(30146..30979)
                     /locus_tag="VBD85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7132969.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(31081..31770)
                     /locus_tag="VBD85_000395"
     CDS             complement(31081..31770)
                     /locus_tag="VBD85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO7132970.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(31925..32641)
                     /locus_tag="VBD85_000396"
     CDS             complement(31925..32641)
                     /locus_tag="VBD85_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7132971.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(32759..33745)
                     /gene="dhaQ"
                     /locus_tag="VBD85_000397"
     CDS             complement(32759..33745)
                     /gene="dhaQ"
                     /locus_tag="VBD85_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO7132972.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            complement(33755..>33838)
                     /locus_tag="VBD85_000398"
     CDS             complement(33755..>33838)
                     /locus_tag="VBD85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7132973.1"
                     /translation="ELINNSKEELFNRKVIEKIIRTSIENY"
BASE COUNT        10608 a         6746 c         5187 g        11297 t
ORIGIN      
        1 tacttgtagt ctttgtatca attttattag actcaatagc tttagcaatc tgtttaatag
       61 tgtaaccctc ggtaattaat atcttaccta aagctggttt tgtcggttct gcagttccgc
      121 ctttttccaa ttcagcagca atctgatcta aagtcatact ttttttaaga ttgtagtatc
      181 cactttgaaa attactatag tttttgaact tggaatagta attaaagact gtggcgctct
      241 taatgactcc tgctttttct aaaatttgac caattaattt attcccagat cctatcggaa
      301 tttcaacttg tacaaattta tttgaattgg aatcaacagg tttaatagca ctatctacaa
      361 aacgataacc tacaaatgct gtcaagagca atacaagtat gataattgat atcagaatac
      421 gtgcaattcg ttttgccaca ttatctgctt tctgacgtct ttcacgcttg ttatgtttgt
      481 tcttggctga caaagctata ccctcttctt cttttgacaa atcctctact cggataggtt
      541 cctttgctac gggctctaac tctgatatct caccaagttt tacattttca ccttctgtag
      601 gaatatctgt tgctctcaca agaaagattt cttccgtttc ttgattaggc acagcagttg
      661 caacatcagt cttatctgct tttaaatcta tgtcaggtac attttcagtt gtcctaaatt
      721 cactagattt ggagccgcta tcaagctctc ttgaaggttc catcccttga ttgataactt
      781 gttcttcttg ggaagctaat gcttgttccg cctgacgtct ttcttcttct cgagcagctt
      841 ctgccagaag tttcgcctca atccgagcct tctcttgctc ttctaagcgt tgcttagtct
      901 ctagcgcctt tgctctagct tcttgttcgt ctttcaaacg ttgcgcttca taatctgcca
      961 tcaattgtgc tgttctgcga gcagcttctt tggcttcttg ctctttttga tagagttctt
     1021 cttcacgcaa ctttctcaaa cggttagctt cttctaactc tgccagaatt tgttctttaa
     1081 aacttttctg atcatggtta gaatgctgat cgtcattaaa ttcggtcaaa gagatttcct
     1141 cctaataata atcacaggca attatatctt aaatagtcta taaaatcaac aaaactttga
     1201 caatgttatc tacttttaac aaattgatcg tgttggatta ttccaaatca tattatacca
     1261 aagcgaacca ccatgttggg agtcagattc tccctcatct ttaaatccat tcatttcata
     1321 gaaggaaatt aaatcatcat ggcaagttaa acttatacca tctcgttctt gagaaacaac
     1381 caggtctttc atcgctgcta ataatgctgt accaatcccc tgacctttaa aatcaggatg
     1441 tattgataga ctagtaattc ctatgaaacc acctaccctt acagggaatt cagaaacttt
     1501 atggaataaa tcgtccgtta agtaccgacc tttaataacc ggtccttcaa tgtaacctgc
     1561 taaacgacca ttaatctctg caactaaaaa agtatcagtc attatcgtta aacgttcttg
     1621 cattgctttt ttactcgcag cctctgcttc tgaaaaattt atttgttcaa tactaatgac
     1681 ttgctgtaaa tcctctaggc aaccatttct aataatcata ctcatctcct taatttcttt
     1741 atgaaattct cttgataaag tcagatagag gactgagaaa cttctctcag cccttaccat
     1801 caacttattg tgtattttta gttaggttag ctaataattc ttcaaaagag cgctcataca
     1861 attgaatgtc tccagcaccc ataaagacat agacagcatt atcatgattg agtaaaggcg
     1921 agacattttc gactgtcact aaatctgagt gtttgacaat cttagcagct aaatcttcta
     1981 ccttcacctc accattatct acttctctag cagaaccata tatttgagcg agataaacgc
     2041 tatccgcttg actcaaggca tgggcaaatt cgtctaaaag agctatcgta cgagtgaacg
     2101 tatgcggttg gaaaatagct acaatttctt ttgacgggta tttttgtcga gcagcatcta
     2161 atgtcgcaat aatctcagta ggatggtgag caaagtcatc aataatgaca gtatcgtcaa
     2221 taatcttctc agtaaaacga cgctttaccc ctgaaaatgt cttcaaatgc tcagctacta
     2281 atgccatatc aattcccatt atgtaaaggt tagcaataac agcagttgca tttaagatat
     2341 tatgtttacc gtatgctggt acatgaaact gaccaatttc ttcttggtta tagaaaacct
     2401 taaagtcaga accattaaca gttcgagtga tgtcttttgc tataaaatca tttgaatctt
     2461 caaaaccata ataatatatt ggtgcctcag aagtgatttc atgaagtttt ggatcttctc
     2521 cataaatgaa taaacctttt tgaacttgct tagcatagtc attaaaggca ttgaatacgt
     2581 cctctaggcc tgtaaaataa tcaggatggt caaaatcaat attggtaata attgagtatt
     2641 ctggatggta cggcataaaa tgacgttcgt attcatcagc ttcaaacaca aagtaattag
     2701 cattagcaga accacgtcct gtaccatctc caattaggaa agaagtgtct gtaatatttt
     2761 ttaaaacatg agctaataaa cctgtcgttg aggtttttcc atgtgcccca gctacaccta
     2821 gactagtgaa ctgacgcata aaatctccga gaaattcatg atatcgttta aaatgatagc
     2881 ccttttcaat aacataagcc aactcttcat tgttatctgg acgaaaagca tttcctgcaa
     2941 taatctctaa atcctcactg atattattcg gtgagaaagg taatatagtt acacctgctt
     3001 gctctaaacc acgttgggta aaataatatt tgtcaacgtc acttccttgg acgttatgtc
     3061 ccatttgatg aagcatcagt gctagggcac tcattccgga tcctttaata ccaataaaat
     3121 gataagtttt tgacatggtc tctccttaat taatgtcttt aaaacgtgtc agatttaatt
     3181 cttgagcctt ggctctttct ttatgaaact gattgtcttg tttattgtaa acttgactgc
     3241 gtttcaaaaa atcaaaatta tttttctttg tttttgggtt cggtgtatta tcaggttctt
     3301 gaaatatgat tggaatttct gctaatatat agctttcctg ctgtaaacga tcagaaagat
     3361 gacttagttc agtcaccttt cgctgagttt gttttttcgg tactgttgcc ttcaattgtt
     3421 gctgaaaagc ttcctttgat ggcgccttaa attcctgttt cgcatagtga gcttgtcgtt
     3481 tctcccgaac agacctcttt gcctcttgtc gagatttgct caactctgtc gcttttttta
     3541 caaaactctg cggtggtaca ggatcatttt caattacctt aggatgtgat atttcttcat
     3601 gttcataatt gcttttgacc atgctttgat atgttttttc ttgataaaag tctcgaatgt
     3661 tgttaattaa atcttcatta tcataaagat tcatctttac aagcggagac attactggtt
     3721 catcatctgc tatcaacgga aatcgtctag agcctctatt cacattatca cttcctttct
     3781 acaacagacc tattataaac taatttttct gttttttcaa agttttcaac taagtaaagc
     3841 ttatcagatt ggtatttttc attcatttat ataaaaaaga aagcgagaga ccactttctt
     3901 ttttatatta tagtcacatc atcatattta tttagaaact ccaagtatct ctcgaatatc
     3961 atctacgctc atgctagcat gggtttcatt tccatccaga actgttgtaa ccaaatgttt
     4021 tttagtttct tgcatttcta gaattttttc ttcgattgtg cctctagtaa tcaagcgata
     4081 aacctcgaca ttctcttttt gccccaacct atgtgcgcga ctaatcgcct gcatttcaac
     4141 agctggattc caccacaagt caatcagaac aacggtatca gctcctgtaa ggttgagacc
     4201 aacgccacca gcttttaaag aaatcaagaa agcatctttt gatcctgcat taaatgctct
     4261 ggtcatttca tgacgttcat ttgctggtgt agaaccagta attttataag ttgtaagccc
     4321 catagctacc atctcacgtt ctgcgatatc taacataccc ctgaactgag aaaaaatcag
     4381 agcacgatgt ccattttcct taatttgtgt taaaagttga cgtaggcttt ctaacttacc
     4441 gctttcacca tcataatcca taaaaagacg tggagtgtca caaatttgac gtaatcgtgt
     4501 gatgccagat aaaatttcaa ttttacgacg atttaagtca gcgtcactag aatgttggat
     4561 actttcttga atttggcgta attgcgccaa atagataact ttttgactgt ctgtcatttc
     4621 atttggatag ttcatttcaa tcaaatccgg taattctggg agaacttctt cttttcgacg
     4681 tctcatcaca aatggtttga tataacgagc aacttgttta gggttcaact tcaaaaactc
     4741 tttcttacca ggtaataaac caggtaaaat gatctggaaa atggaccaaa tttctaacag
     4801 tttattctca ataggtgttc ccgataatgc aaaacagttt ttgacctcaa aagatctcaa
     4861 gctatgggct attttagttt gagcattttt cataacctgt gcttcatcca aaattaaata
     4921 atcataattt ccttcagaat aagtctcaaa atcctgccta aatgatgaat aacttgtaat
     4981 gattacttga tgacgttcag caataatttc atctctacga atcttagaac cataggcaac
     5041 tgctacatct acgtctggcg caaatttgtg gaattcatct tgccaattat aaattaaact
     5101 tgatggtgat aaaataagca cacgagaatc tctagttagt tttgtactta aaaatgaaat
     5161 cgtttgaagt gtttttccaa gccccatatc atcagctaaa atccctgcaa agccataatg
     5221 atctaacata cttaaccaac ggacacctgt aagctgataa tcgcgcaagg aagctgaaac
     5281 agatagacca ggtattttaa acttttctgg gtgtctcaaa tcttcaatca attggtaaaa
     5341 atgttgtgaa aagctaacat tatcttgatt tgcaaacagt tctgataatt gaaatgctgc
     5401 aatattatct aattcaatat gcccattctt tgctcgtcta gctctcaacc cttgcaaagt
     5461 tgcacttact ttcttagttt cttcatcaaa aataactaat tgccctgatt tattcacaaa
     5521 ataaggatta ttgtcaaaca aagctaccag agcttgatca acatcgtttt ccagtaaatc
     5581 agagaaatca aaagaaatat ctagcaggct tcccttagct tcaatatcaa tttgcggtcg
     5641 ttctatgaga cgataacttt ccaattttgc ggataagctc actgttccca tttttttaaa
     5701 ttgtggaagc atataggtat aaaaatcgta taattcttgt gcactgtatg ctggatggct
     5761 ggttgaaaag tggggtgcaa agccatattt ttctaagagt ttaaatactt tttcttcatg
     5821 tttgaagtga ctagcaaatg ttaactgttc tagttcttga cgattatgaa cagttaaatc
     5881 attaccataa tcaaatacca tttgcaataa aatttcattt tgactattaa tatcgaactc
     5941 aaaatttacc ttaaaatcat gaatagagaa gctccgtgga gcatccacta gtccaatctc
     6001 cttaaaatcc agtaaatgag ctgctaactt atcttgatca tctaaatcaa aatggatatg
     6061 ttttgctaaa tctccctcaa tgggaagact acgaatagcc gttaccatct tttcttgctt
     6121 caaattaaga tggtagaaag tatcgcgata aaacaaataa gaattcgcaa atagtggttt
     6181 taagtctttc tctttgattt ctaattcaat tgattgccta tggacaataa ccttaaattg
     6241 ataaagatga tcctcacctt ctaattcctt aaagtagagg tgattaaatt gatgaaaatc
     6301 actttcaaat gaaaaatttt ctaaagcatt catcaaagta acaccctctt caaaaaaacc
     6361 acgagtgaga ctaaggtgac gaccttgatt tggtaaaata aactctagat caattttgct
     6421 tgaatgactt ggaatgagcc tccataaaaa ttctatcaat tcttgactgg tctcatcaaa
     6481 ctgtattaaa gacaaagttt caaagtattg ttttccaatc tgataataag cttcttttcg
     6541 aacagtattt aaaaaggctt tgatgtcacg aataacataa cttctatcat caggcagtcg
     6601 tctaattttt aaagtccacc agatatcatt agcataagga ttttgttctc cttgagccga
     6661 taattgatat tttatagtat catcttcatt aataattaga ctatctaaaa aaacactacc
     6721 aaaagaggtt ttcttcttag tttcttgttg actttcttgt tgaacttgaa ctttagataa
     6781 gattgcttta ccttctggat cgtttttcaa gaaatgctcc agagcagcta aatgttgaca
     6841 atagcctttt cttgcaaaaa aatcacaaga acattttgtt tcttctgtta ctaatgaata
     6901 ttcaatctga caatccccaa ctttagcttt taatagatta ccttcttgag aaatcaagga
     6961 caccaaacct tgttcgtaga gttctatacc ttgatttcga atacgtcctg gaatcattct
     7021 actcattatg ctaccacctt catcttatcc cttaattata acaaaattca agtccaattt
     7081 cttgtaaaaa tactaaattg attgtttttt gatacagtaa caaataaaaa aggctaaaac
     7141 aactgtccta accttctatt tcaatttcaa caataagatg ttatttacgt ttacgtgcta
     7201 ttagattaat tggtgttcct tcaaaaacaa atgcttcacg gatttgattt tctaagaaac
     7261 gaagatatga aaagtgcatc aattcttctt cgttaacaaa aacaacgaaa gttggtggtt
     7321 taacagcaac ttgagttgcg tagaaaatct taagacgttt tcctttatca gttggtgttg
     7381 gattaatagc aatagcatcc ataatcacat catttaatac agcagacgga atacgtttgt
     7441 tttgactttc gctaatacgt ttaatcatat ctggtaactt gtgcaaacgt tgctttgtct
     7501 ctgcagaaac aaagataatt ggcgcataag acaagaattg gaagttatca cgtatatcag
     7561 cttcccactg actgacagta tgattgtctt tttcgatagt atcccattta ttaactacaa
     7621 taataatacc tttaccagtt tcatgtgcaa accctgcaat acgcttgtca tactcacgaa
     7681 taccttcctc tgcattaatc accataagaa caacatctga gcggtcgatg gcgcgcatag
     7741 aacgcataac agaatatttc tctgtgtttt catatacctt accagattta cgcataccag
     7801 cagtatcaat catagtatac tcttgcccct ggctatcaac gaaattagta tcaatagcat
     7861 cgcgcgtagt accagcaact gggctagcaa taacacggtc ttccccaagg atggcattaa
     7921 ttaaactaga tttcccaaca tttggacgtc caatcaaact aaaacgaata atatctggat
     7981 tttcattttc ctcttctacc ggaagatttt ccacaatagc atccaatata tcaccagtac
     8041 caataccatg aactgatgac aagggataag gatcccctaa accaagagag tagaaatcgt
     8101 aaatatcatt gcgcatctca ggattatcta ccttattaac agctaaaata accggtttat
     8161 tggtcttata aagaatacgt gagacatact catctgcatc agtcacacct tctttacccg
     8221 acacaacaaa cacaataaca tcagcctctg tcatagcaat gtctgcttgg tgcttaattt
     8281 gctccataaa cggtgcatca acatcatcaa tcccaccagt atcaattaat gaaaacttac
     8341 gatttagcca ctcacccgtt gtatagatac gatcacgagt gactccttca acgtcctcaa
     8401 caatggaaat acgttctcct gcaattctgt taaataatgt tgattttcca acattcggac
     8461 gaccaacaat agcaactgta ggtaatacca taattctcct ctcactattg ggaatactcc
     8521 caattccatt ctattttatt aacggcgatt cggtccttct aagtggaatt ctatcgctaa
     8581 ataccgtacg cgctccataa ctcgttttgc ttgccaagtt tcgtcgcttc cttttatatt
     8641 agcccatttt ctttccaaat cgttaaaact ataatttgac gtaaagaaag tgggcaactc
     8701 ttctagcata cgatgttgta atataacttg taaaatctcg tcacgaaccc aggacgttgc
     8761 ctgttcagcc ccaatatcat ctaaaatcaa aatcggaaca cttttcacag catcaatttc
     8821 atcttttaca gttccgcttg aaatagcatt tttgacatca atagcaaaac tagggaaatg
     8881 aagaagagtc gttgacacac cctttctctc tgataattcc ctcgtcattg ctgccattaa
     8941 ataggatttc ccaaccccca tatctccata taaataaagc cccttgtgat tatatgaagg
     9001 ataagtctct acaaaatcaa gcagttgtga catagctttc attcgactct cgttattaat
     9061 atcaaagtca gtcattctga tattccgata ggacttgggt agattaacta aattaatacg
     9121 atcactgata gcttgttttt tttgtgcttc aattaactct cgtgtttcta aataagaaac
     9181 atctgcataa ccttcattca taaccaaaat aggctcatag cctttagcaa tatattgact
     9241 atccttattt ttaaatttct gacgctcaat caaaaattga ttaaatttag acatgctgat
     9301 atttatttca cgctgagaaa gttggtgggt tttaatgaac tccgctacct gagcatctgc
     9361 taaaatcatt tgaataagct catcatttgt attacgaggc actctccctt gattttctaa
     9421 tgcttgtccc actgatttca tgattcacca tcctttccta atcgttctaa acgcttgagg
     9481 gcatctgttt taaactgttc taattcaatt tctttttctg ggctaacctc atctttataa
     9541 tcaggattac tccattcagg aacattcgat tgtttcttct tcgtttctgt tttagtcctt
     9601 actttttgat ctgaaaaatc acgaatttta agcacagcat cttcggccgt cataacattc
     9661 tgaaaagcaa aatcattagc aactttcatg atataggcct tatttaagtt agcagatttt
     9721 gtcttattca aggtatataa aaccataaca ttaatgactt cgtctaggaa attcattttt
     9781 gccaaatcct caagtaaagt cttctcacca cttgttgtca ctgccttacg gcttctcttg
     9841 attttttcaa ggaacactag agcactatca tttttagatt cccttagtat gaccttttca
     9901 ttatttgtaa aactctgatt atctttaata tgctgcccct cattttgttg aactttcatt
     9961 cgctgaggcg caatcattcc attgatagct gtttgttttg ctaattgata ggtatcatac
    10021 caatttaaat gataaagttc tgatacacta taaataccta agacatcatc tttttcattg
    10081 ttaaaacgta agccatctcg catcatcaaa cgtttgaaac tttccaagtc aaaaacattt
    10141 ttactccggt ttacctcttg ttttggttgt cctaaatccg agaaaacatc tgtaaatttt
    10201 ttagagatat ctctagcatt tttaggtatt ttcatatcta attctgccac tgctacctct
    10261 cctatacgtt tttctaacaa acgacgatag atgggattag ataaaaataa ctcatttgac
    10321 atagcttgat tcaacttaac aaggtaagta cctgtggctt ggtaaacagt caacaaatcc
    10381 aaagctgtta acattaccaa tgcatcttga aatcttggca tcccgtattg cagatgattg
    10441 agaacctctg aaaatttatg acgtcgtatt ccgtcatcaa aaaaatgaac aaaataatca
    10501 tagagtgcca tggcatcact tcccataatt gggaaataca attgtattaa actattacta
    10561 tcataaacaa tcttattttg cttaatatat gtaaactcat ctattggact catcacacgc
    10621 taccactctt tttactccgt acgcgctttg taatttgttg aagtagttct tcaatttcat
    10681 ctacatcttt aaaagattta taaacactag caaagcgcac ataagtaatc tcatctaatt
    10741 cggcgagttc atccatcact aaatttccga tagtgatact agaaacctca tcttcatact
    10801 cactcctaat tttacgttcg atacgtaaga tacaattttc aatatcttcg ctcgaaacag
    10861 ggcgtttttg agcactctga ataataccat tcaaaatttt atctcgagaa aattgctcgc
    10921 gagtaccatc tttctttatg actaaaagag gcaattcttc caaacgttca aatgttgtaa
    10981 agcggttgcc acatttttcg cactctctcc ggcgtctgat agtcgtacct tcttcagctt
    11041 gtcggctatc aaccacacta gatttattat agccacattt tggacaacgc atgtcaacac
    11101 ccctattcta tcatatcatt taatcatctt attttaacac aaattcagta ataacaaaag
    11161 aagcgcctct cttatcacct catcatatca aaacacaaaa taatccttat gccttttatc
    11221 attaagcata aggatttttc ttatatttct ttagtttctt caaatttttt tataggaatg
    11281 cgaacaatga attcagtccc ttgatttagt tcacttttaa tatccacctt taaatggtaa
    11341 gcatccataa tttgtttaaa cactgaaagc ccgataccta aaccagcttg tgtactttct
    11401 ctgttacgtg atttatcagt tcgataaaaa cgatcaaaga taaactcaat atcttcatct
    11461 gagatacctt ctcctttatc ttttacaact actgtcgcaa aatcattgtc tactgataaa
    11521 acaacactga cttctttata actaggagag tacttgatag cgttatcaat taagatcatg
    11581 agagcttgct caaaatgtat cttatatatc tttccccaaa cgatatctga aatattattt
    11641 tcaaaaataa attgaaaatc ttctctcaaa atacggaaat tttcaataac aactgatata
    11701 gagcttgata aatctgtcac ttcatcttga tgaagttcca agcttccttg gacgcgaatc
    11761 atatttaaca tgtcattaat cattaaggac atacgatctg cctcatggta tgccgctgct
    11821 aaactttctt caagaatttc tggatcatct ttcccccaac gttgtaataa accaatatga
    11881 ccctttacaa cagcgacagg cgttcttaat tcatgactaa catcactaat gaattgtgat
    11941 tgtcgacgag tatagtcttc caactgatcc aacatcccat caaaaatcct agacaattct
    12001 ccaatttcgt cttctgaacg gaccttactt ctaatttcta aattactagg attttcagat
    12061 atctgatgca ttaagtcgtg taaattttta accggacgaa caatacgctt agtagcacta
    12121 ttaataacga ccaaggctaa aacagtccct aaaacttcca tcataatcaa gaaaacaatg
    12181 atgttttgcc tcatactata ataacgacct aaattataga aaatttgaac gtatccaagc
    12241 aactgtcctg ttttttgaga gtagattgct tttgtaccaa caaaaccttc catatctgca
    12301 actttagaga tgataacttt tcccatggat ttaccaatag ttggcatacc cgttctcagt
    12361 gtagaaaaaa ctggtttcct gttgacatcg tatagggtca tttcttgatt aacataaaga
    12421 atactatcga ttgactcgcc cgttctaaca attccatttt gacgcttatg aggatagatg
    12481 tcatccgcaa aaacctggtt gtacaggatc tctaaaatat catcggatgt caaactagag
    12541 ttcgcttgcg acaaacgaac tctagttttt tctaatgacc taccgacatt aattttttcc
    12601 ctgtgaagga cgtatcttgt tgccgaagta taaactaaaa cagaaaatac tgtaaatagg
    12661 ctaaaaaata agataaaaac taattgcgat aatttttttg acagaggttg ttttacccct
    12721 ataaactgat cttttttatt tttcacgaat cacatagccc attccgcgaa cagtttgaat
    12781 atatgattca cgacctggga tatcaatttt acctcttagg taacggatat aaacatcaac
    12841 aacgtttgtc tctgctgcca catcgtattt ccaaacatgt tctagcaact cttctcgtgt
    12901 cataacacga ttcatatttg tcatcaagac attcaacaaa tcaaattcac gttttgttag
    12961 tgaaatttcc tcatcaccac gcatcgctga acgattatga gtatttaatg acaaatcacg
    13021 gaaactacca ctatcacctt tttcttttgt cttcgtttca atttcttggc gtcggaaaat
    13081 cgctctaaca cgtgctaata attcttcgat tgcaaacggc ttaacaatat aatcatctgc
    13141 tccacgatca agaccagcta caatatccat aacagaatca cgtgctgtca tcatcatgat
    13201 ataggttgtt ttttcagcct gcaggcgacg tgtgatttcg aaaccatcca tctctggaag
    13261 cattaaatcc agtagaatca aatcaaaatc tttttctaat gctgtgtcca atccttcacg
    13321 accgtttgtt tcaacaacaa catcatatcc ttcatgtagt agttctaacg agacgaagcg
    13381 agctaaattt ttctcatctt cgattattaa gatcttttta cccatctaca ccaaccttct
    13441 tattctttac ttaacactat caattatttt ttagaatatc tagataaaaa acaagatagc
    13501 ccacatttta ctaacgtttc tttaaataaa taaatcgcca tcccaaacaa ttgttcgata
    13561 gcgagactta atttttatta ttgagtagtg tcctctgtca cttttcaatt ctactaaaaa
    13621 aaaattaaaa tatcaactga tgttattaat aatttttatc taatcactat caaacatacc
    13681 ttctaaggct gcgaaaggat tagatttttc tttttcttta gcttgttttt cttcatactg
    13741 cttttcagtc attaacgacc aatttttacc tgataaatcc gcttctaccc caacctcatc
    13801 agctgccaga acacgaagag gtatatttaa caagatatta tcgataacgc tttcttctaa
    13861 attaatatca tcttcctcta atattaagac caaatcctga tcaacgagct cttttttagt
    13921 gctaacattt tcagcttcaa taaaaacttc attaatagtt tgcttttcag ataaaacaac
    13981 tggtttcata gaacgactag acggtaatgt aatatcgtaa gccatattat agtcaagtag
    14041 atagagacca tcttcataca ccacatgacc tgaaacttga atatcagaaa tatccattat
    14101 ctctgaatga cgctccataa gcgactcttt gatatctatt tttttatcaa aataaagccc
    14161 ctctggtgat tttttgattt ctgtaagtaa cacgtgccat ctccctttat agtatatgtc
    14221 atattatatc aagttttaca ggctttgtct taaaaaaaca aaacgcaagg catagctacc
    14281 ctacgttttg ttaacgacta catatgacgc aaacgctcaa tacgatctgc aattggtgga
    14341 tgcgtataaa ataaagactt taacccttct tttttcgttg ggtcattgat atagagagcc
    14401 gcactagcat catctactgg atgccccata ggttcagaac gatcaagttt ttctaaagca
    14461 gagatcattc cttgtggatt tcttgttaac tcaacagaac tagcgtctgc taagtattcg
    14521 cgctgtcttg aaatagctaa ttgaactaaa ctagcggcta atggtgcaag gattagtgat
    14581 aaaatagaga aaatcaaaac caagatgttt ccaccatctt ctctgtcatc atcacgccta
    14641 cgacctccgc cgtaaaataa catacggcta ccaatgcttg atattagagt tacagcactc
    14701 gctaaagcta ctgcaatggt tgaaatacgg atatcataat tacgaatatg actaacctca
    14761 tgtccaataa caccctctag ctcttcgcgg ttcataacag ctaataggcc tgtcgttgct
    14821 gcaacagcag cattttctgg cttagagcct gtggcaaagg catttaggga gtcatcttca
    14881 acaatgaaaa cgcgtggcat aggtatctga gctatcattg ccatatcttc aacaatatga
    14941 aaataatttg gcgcctcatc ttctgtaact tcacgcgcat tgttcattga cataacaaca
    15001 ttagttgatt gaaaaatcat acttactgca tagataaccc caataattag agctagaaca
    15061 agcccaaatt ggtagctgcc taataccaaa taacctacag ctgctccaat agctgctaaa
    15121 agacagaaga agacaattaa caagacaact gtttttctct tattacttgc aatttgttga
    15181 tacagcaaaa gcctctctcc cttctcaatt aaaagtcaaa ggaaactttt ggcacttctt
    15241 tttcttcttc aggagtttct aagaattggc ttggtttgaa cccaaatagt tttccaacaa
    15301 tattacttgg gaaagtttct aatttcacat tataattgga tgttgttgtg ttatataact
    15361 gacgtgaata agaaatttta ttttccgtat tagttagttc ttcttgaagt ttaacaaagc
    15421 tattgttggc ttttaaatct gggtaatttt cagcaaccgc taaaatactt gaaagctgtc
    15481 ttgttagctc attacttgct gtcattgctt cagcaggtgt gttagcttta gcaacttgag
    15541 cacgcagttc tgcaatcttc tcaagtgttt tcccttcgta cgcagcatag cctttaacag
    15601 tttcgattag gtttggaatt aaatcgttac gacgtttcaa ttgaacgtct atttgactcc
    15661 atgattcttt agtatgcatg cgacttctaa ctaaactgtt ataagcaaca atcaaccaaa
    15721 taacaaacaa agcaataatt gctattaaaa tcatagttcc catagtagta gctcctttac
    15781 attttttcca ttatagcaat tttctttaga ttaggaaagt cctgttattc ccattattag
    15841 tatttgtgat aaaatagagt cattatacga tatagaaatt gaggtttact atgacacccc
    15901 aagcatttta tcaagttctg atagagcatg gtattacact tactgacaaa cagaaaaaac
    15961 aatttgaaac ttattttaga cttcttgttg aatggaatga gaaaattaac ctaacagcta
    16021 ttacagataa ggaagaggtt tacttaaagc acttctatga ttctattgcg ccgattttac
    16081 aaggttatat tgacaatagt cctcttagca ttttagatat tggtgctggt gcaggttttc
    16141 ctagtattcc tatgaaaatc ctttatcctg aaattgatat tactattatt gattcactga
    16201 ataaacgcat taattttcta aacatattag ctaatgaact tgaactttca ggagttcact
    16261 tcttccatgg gcgcgctgag gactttggtc aagatagagt atttcgagct aaatttgata
    16321 tcgtaacagc tcgtgcagta gctagaatgc aggttctagc agaattgaca ataccttttc
    16381 ttaaagttaa tggtcgcttg attgcactga aagctgcagc tgctgaagag gaattaattt
    16441 ctgcagaaaa agctttgaaa acactatttt cacaagtaac tgtaaataaa aattataagt
    16501 tacctaatgg tgatgaccga aatatcacaa tcgttagcaa gaaaaaagaa acgcctaata
    16561 aataccctcg taaagctggt actccaaaca agaagccttt gtaaagttgg gagctagtat
    16621 gaaacatttc tttgattaca agacaatgtc tgtcgcacgt aaactcagca ttagttttat
    16681 cgctgttatc ctattgggaa gtattttact ttcacttcca atttttcaat atgctaacgc
    16741 ccctaaaaca cattacattg atcacctttt cacgactgtt tcaatggtat gtgtaactgg
    16801 tttatctgtt tttcctatca gtaaggttta taacggttgg gggcagattg tagctatttt
    16861 actcatgcaa acagggggac ttggtttagt aacactaatg tcgttgtctt actataccct
    16921 aagacgtaaa atgtcgttaa atgatcagac ccttttgcag tcagcgataa cttataacag
    16981 ttcgacagat ttaaaaaaat atctctatat gatttttaaa gtaactttaa ctttagaagt
    17041 tttagcagcc agcattttag ccatagattt tattcctcga tttggtttgg gtcatggtat
    17101 ctttaatagt atattcttag ctgtctcagc cttttgtaat gccggttttg ataatctagg
    17161 ggcaacgagc ttagctcaat ttaaactaaa tcctttggtt aacattatcg tttgtttctt
    17221 gattatttca ggaggacttg gatttgccgt atggaaagat ttaattgaag ctactataca
    17281 aacatctcac aagggaccaa aattaatcaa gacatttcca aaacgcctca gcaaccattc
    17341 taaactggtt ttaaaaacaa caaccattat tttactaaca ggaactttgc tttcttggtt
    17401 gttagagttt ggaaatttta gaacaattgc taatttatct ctacctaaac aactaatggt
    17461 cagctttttc caaacagtta cgatgcgtac cgctggattt tcaactattg attataccca
    17521 atctgatttc gcaaccaacc ttgtttatat tatccaaatg ttaattggag gagctcctgg
    17581 tggaacggca ggaggattta aagtcactgt tatcgccatt ttactcttac tatttaaagc
    17641 cgaactgagt ggccaaagcc aagtgacttt tcattatcga acaattcctt ccagtattat
    17701 taaacaaaca ctatcgattt taactttctt ttttatcata cttattagcg gctatctcct
    17761 tcttctggaa ctcaatcccc atatagatcc tttttctctt ttctttgagg catctagtgc
    17821 cttagcaacc gtgggtgtaa caatgaacac taccaatcaa ttaactctgg gcggtcgaat
    17881 tgtaattatg ttcctcatgt ttattggtcg agtcggtcca attaccgtac tgctatcaat
    17941 tcttcagaaa aaagagaagg aaattcacta tgctgaaaca gaaattattc taggttagaa
    18001 aaggtctttt atgaaaacaa aaattattgg tgtacttggt ctaggaatat ttggacaaac
    18061 actcgcacaa gaactaagta actttgaaca agatgttatt gctattgaca gcaatcctga
    18121 aaatgtacaa gctgtcgccg aagttgttac aaaagcagct atcggagaca ttactgattt
    18181 agctttccta aaacacatcg ggatcagtga ctgtgatact gttattattg ctacaggaaa
    18241 cagtttagag agctcagtat tggccgtaat gcactgtaaa aagttaggcg tcccacaagt
    18301 tattgctaaa gctcgaaacc ttgtatacga agaagtactt tatgaaattg gtgctgattt
    18361 ggttatctct ccggagcgag aatctgggca aaatgttgct gcaaacctca tgagaaataa
    18421 aattacagat gtcttccaga ttgaatctga tatttctgtc attgaattta aaattcccaa
    18481 atcatgggta gggaaaactg tcgaacaatt aaatatccgt cacaagtttg atcttaacct
    18541 cataggtatc agaaaagcaa aaaacaaacc tgtcgatacc gaagttccaa tcaatagtcc
    18601 acttgaagaa ggtattattc tagtggctat cgctaactca gatgccttcc aacgctatga
    18661 ctatttagga tactttaact aaagaaaata aaaaactgac tattaaggtc agttttttat
    18721 tgccatgggt tatagaaacg tccatgatta aggtgaatta aataaaggct tacaaacaga
    18781 ctagctagag caagtatgat taagacaatg tcactttttt ccaaagattg cttagagtac
    18841 caagtacgtc ttttattttt accaaatcgt cgaagttcca tagcagttgc tactgtatca
    18901 atacgttcta gagagctaaa aattaatggt gtgacaatct gcaaattccc cttaatgcgt
    18961 gctactaagt tcgatttttt tgataattct attcctcttg cctcttgtgc tcgacgtatt
    19021 gtataaaatt cttcttgaac atcaggtata tatctaaggg tcaaactaac agcataagct
    19081 attttatatg ataaacctaa ttgatttaaa cttgatgcga actgactagg attagttgtc
    19141 atcaaaaaca aaagagctaa aggcactgta caaaagtatt ttaaaatcag attaaagagg
    19201 taaaacaact cttggctagt cacatcatat gggccaattc cttttaacaa taaagaagaa
    19261 ctaccataaa tcttatcacc ataatgggga gcaaaaagat agaccattat aatatttaaa
    19321 acagcgaata caacaactaa agtcataaca aaggacacct gttgccattt gatttttgct
    19381 aatctaaata agactagcga tcccaatgaa acaacaatta aaaatcttgt atcataagtg
    19441 gtcatgcaag caacagaaac taaaataaaa aatagtaact tactggcacc tgatagcctg
    19501 tatagaaagc cataaccctc ttgataacca attaattgtt tagccattct tacctccttg
    19561 attatcaatg taccattgtg ttagtttctg tggagatatc cctaatctgt ctgctaaagc
    19621 aaataaactc gttttcttta aatgagtctt tttcaaaagc tctgtatttg aaaggatgtt
    19681 aataggatga tcatctgcaa caatcagacc attgtcgatt acaattgcac gatctgtata
    19741 ctctaacata agctgcatat catgtgtaat cataacaatg gtatgtccat cacaacttag
    19801 tttatcaagg aaagacatca tttcagtata atgcttcata tcttgaccag ctgttggttc
    19861 atccaaaata ataacttctg gatttaaaat cagtatcgaa gcaatcgtaa cacgtttttt
    19921 ctgaccgaat gatagagccg atataggcca attcctaaat tggtagagcc cacaaacctt
    19981 taatatatcg tagacacgac tttctatatc attatcacta aaacctctta aacggagacc
    20041 cagagcaact tcatcaaaaa ccatcgcttg acttatcatc tgattgggat tttgcaaaac
    20101 atagccaatt cgttctgctc tttcttttat agagtcttga ctgacatctt cattattata
    20161 aattagagta gcattattag ggataaattg acataacgct ttagctaaag tagatttacc
    20221 tgcaccattt ttcccaacga tagctaaccg ctgaccttta tataaatcaa acgaaacatc
    20281 atctaaagta ttattctcta aatcatatga caccgacaaa tgctgtactg ataataattt
    20341 atctgtatgt ttatctaaat ctaatgctcg cattttaggt atcaatagtt cactgaaatc
    20401 aaagtcatca atagacgtca ttgtattttg tttttcaaag tcatatccta aatttttgag
    20461 agcagaaata tacagtggtt ctcgaatacc atattcagat aaaaaattag tttttaaaag
    20521 ttgatccggc tccccattat aaataatttg tccatcacta aaaagaacta cacgatctac
    20581 tcgctcataa aaaacatctt ccaatcgatg ttcaataata atagtggtcg catcttgctc
    20641 ttgatgaata cggtcaacca atgccatgat atcttgtccc gattgtggat ctagattagc
    20701 caaaggctca tcaaataata gaattgggct atcgtctact aaaactccag ctaaactaac
    20761 ccgttgtttt tgtcctcccg acaagtcttg tggacgttta gacaaaagag gagctatttc
    20821 taaacgtttt gcccacattt caacgataga ggccatttct tcctgtgcta caacatcatt
    20881 ttctaatgca aaagcaatat cttctgctac tgtcaatcct ataaattggc catctggatc
    20941 ttgtaaaacc gtagaaacta aatgagactt gtcatatatt gacaagtcaa aagcattttt
    21001 atggttaatc gtaaaaatac cactatattg gcctttatga gtatttggga taataccatt
    21061 taaacaatga ccaagcgttg acttccccga ccctgaaggt cctaatatca agaccttttc
    21121 acctttagga atgcttaagt taatcccttt taaagttggt tcagattgaa cgtcatattg
    21181 aaaggtaaaa tccttccatt caataaaatc tttcatagat tactctctta tcctaatctt
    21241 tggataaact acctttttgt ggtcgtgatt tagcgtatgc tagcaacaaa agcgtaccac
    21301 ctactccaat agtaatggaa ttcacaaggc ttgataagaa accttgtgca aaaactttgc
    21361 ttgcaggttc gctataaaag aaaatatcac catacggtgc aacgacagac caacctataa
    21421 ggttagcaat aacttgaact acattaaata gaagcaaatc cttcttagtc atgccagata
    21481 attgaatagc taatttttta gcaaaaaagc ctatcatcaa acctaaaaga ccactaacta
    21541 aaacccaagt ccaccacgga gaaccatatt gaatcgaatc tttcaatgcg tgtccgataa
    21601 aaccagtaaa aaaaccaact cctggtccat aaataactgc aaataatgct aaaactgcat
    21661 actgcaattg aatattagta tttggaattg gtgttggtat attaactagc atacctataa
    21721 tgataaaaag tgcagctcca attccagtcg ctacaacctt ttttattgtg tttgtattca
    21781 taaaaacctc tctgaaaaac aagcttaaac agtcacttac aatctgttct attttatcat
    21841 atttcaagct gatttgaaac cttattgaca agaagaactg aaaaacgatt gaaaataata
    21901 ttaaatacca aaaaagatct cagatagaat acctaagacc ttcattagtt tatttttttc
    21961 caaaaactga tttttcagct acagccgtga acagaacgtc tgtcgatgaa tttaaggctg
    22021 tctcacacga atcctgtatc acccctacaa tgaaaccaac tcctacgact tgcattgcaa
    22081 cgtcatttga aataccaaat aagctacatg ccaccggaat caataaaagt gaaccaccag
    22141 ttacaccaga agctccacat gctgatacag cagcaaccac acttaataag aatgctgttg
    22201 gaaaatcaac agtaattcct aaagtattta ctgctgctaa agtcaggata ttaattgtaa
    22261 tagctgcacc agccatattg atagctgcac caagcgggat agatactgag taagtgtctt
    22321 tatctaatcc taaatcctca catagtctca tattaacagg aatattcgcc gcagaactac
    22381 gtgtaaagaa agctgtaata cctgaatcct ttaagcatcg taataccaaa gggtaaggat
    22441 ttttacgcat cataacaaaa gcaatgattg ggttaacgac aagagctaca aataacatcg
    22501 tacccaccaa aactaatatt aaaagaccat aatcacctag tataccaata ccgttttctg
    22561 aaatgctagt gaaaaccaac cccataatac caattggggc caaattgata atccatttga
    22621 caacttgtga agtagcatca gcaaaagttt gcattaaatc ttttgtacgc tgattactgc
    22681 ttcgcatcgc caaaccaaaa ataaccgccc aaaatagaat actcatataa ttggcctcgg
    22741 taatggcatg aatgggatta tcaacaattt tcaacaaaag agattgaaag acttctgaaa
    22801 tccccttggg aggtaataag tctgtctttg ctggcgtttt caatatcagt gtcaaaggga
    22861 aaatatagtt cacaacaaca gcgattaggg ccgctgcaaa agttgcagtg atatacaaac
    22921 caattatcgt tgacatattg gtcttaccgc cctcacggtg ttgcgacaag gcactaataa
    22981 ctaaagtgaa aactaaaaga ggggcaatag ctttcaaccc tcctacaaaa agttgaccaa
    23041 gtaaaccaat cgcagaggct ttagggacta aaatacccaa gaataagcca ataacgactc
    23101 caattgttat tttttttatc aagcttgttt ttctccaagc ttttaaaaat cgtttcatac
    23161 taaactccta aaaaattctt aaactctatg atatgataaa aaaaaagatt cgtcaaatac
    23221 cttttttatt ttttagaggt tgacgaattc taaatttttc tgatataatt actttttgaa
    23281 atattcaatc aatttcgaaa ataaaatatt tttttgtaca gaaaggtaca ttgatgaaac
    23341 taaaaaacac ttatatcatt atcggcttga tgctttttgc gctgttcttt ggagccggta
    23401 atcttattta tccagcctac ttaggtatct actctggttc taattgggcg tgggctatca
    23461 ttgggttctg cttaacaggc gtaactttac ccctacttgg tgttgcagct gtggcgtact
    23521 caggagcaac cgatgttgaa agcttcacca aacctgtctc taaaacatat gctattttct
    23581 ttgcagtagc actataccta tctattggac ctttctttgc cattcctcga acaggggcaa
    23641 cctcattctc agttggtatt caacctatct taggcgacag ctccttagtc aaaattatct
    23701 atggtttaat tttctttggt atttcttatt tcttagctat tagacctagt cagattgtag
    23761 atcgtattgg taaatatcta actccctttt tactaactgt tattagtatt ttaattattg
    23821 cctcactcat ccatccagct gctatcgttg gaacaccaca taacgcttca acgaatgtca
    23881 gcgatgcttt caaggattta ccattcctag caggactgat tcaggggtat ggtacaatgg
    23941 atgcccttgc atcaattgct tttgctatta tcgttattca agcaagtaaa cagtacggag
    24001 caattaccaa aaaagaaatt acttctatgg cacttaaatc tggtgctatc gctacatttc
    24061 ttttagcttt tatttatatt tttgtaggac gtattggtgc aacttcacaa agcttgttta
    24121 aatttgcaaa tggttccttc ttacttcaca atactccaat agacggtggg catgttctta
    24181 gtcaatcagc taacttctat ttaggaattg tgggacaagc tattttaggt acggctattt
    24241 ttcttgcttg cttaacaaca gctactggat taatcactgc ttgtgcggaa tacttccaca
    24301 aattacttcc aaagattagt cacattactt gggctacaat ctttacttta attgctatta
    24361 cgttctattt tggtggttta tcagagatta tccgttggtc actaccagtt ctctaccttc
    24421 tctatccatt aactatagtc ttaattttct tagtcttttt tgatcaaaaa tttgaaagta
    24481 gccgcattgt ctaccaaact tctattgccg ccactgctgt cgctgcttta tacgatgcct
    24541 tatcaaaact cggcgaaatg actggtttat ttaccattcc atccgctttg accacattct
    24601 ttactaaagt tgttccttta ggtgaatatt ctatgggatg gattagtttt gcaatttgtg
    24661 gtgtcttagt tggtcttatt ctaaaaaaag taaaagcata ataatttaaa gcagtacgaa
    24721 aattactgct tttattttaa aataattatt gtaaccgctt tcttttggtg gtataatagc
    24781 cttgaaaata aataattttt ggaggaactc taatatgaga gctgcaactt atctttcagc
    24841 taatgaatta tcacttactg ataaggctaa acctcaagta ataaaaccca cagatgcggt
    24901 tgtaagactt gtcaaaacaa ccatttgtgg tactgatcta catatccttg gtggcgatgt
    24961 tcctgcttgt aaaccaggaa caactttagg tcatgaaggc attggtatcg tcgaagaagt
    25021 tggttctgct gtcaatcatt ttaaagtcgg agataaagta attatttctt gtgtcactgc
    25081 ctgtaatact tgctattact gtaagcacca gttgccttca cattgtgaag atggcgggtg
    25141 gattttagga catcttattg aaggtactca agcagaatat gttcatattc cacatgcaga
    25201 tggtagcctt tatcatgcac ctgaaggtgt ttgtgacgat gcattggtta tgttatctga
    25261 tattttacca acttcatacg aaatcggcgt cttaccttct cacattaaac caggtgatac
    25321 tgtttgtatt gtaggcgctg gaccaattgg actatctgcc ttactaactg cacaattcta
    25381 ttctcctgcc aaaattatta tggttgattt atctcaaaaa cgacttgaag cctctaaaaa
    25441 atttggtgca acacacacca ttctatcaac gtctactcaa gaagttaaag aagaaattga
    25501 taaaattacc aaaggccgtg gcgttgatgt cgtattagaa tgtgttggtt atcctgcaac
    25561 atttgatatt tgtcaaaatg tagtttctat tggaggacat atcgcaaatg ttggtgttca
    25621 tggtaaacct gttgaattta acctccaaga tttatggata aaaaatataa cccttaatac
    25681 tggattagtt aatgctaata caactgaaat gctccttgaa gttcttgaga caggcaaaat
    25741 cgatgcaaca caactagtaa cacatcactt caagctatct gaaatcgaag aagcttataa
    25801 ggtatttaaa gctgctgaag aaaacaatac tctcaaagtt atcatcgaaa acgatattac
    25861 tccagcctga taatttttaa acaaataaaa atgtgagaag aatagccttc tcacattttt
    25921 atttatgact caagcgacgt gttaaaaaat cacctaaaaa ttggataaag aaaattaata
    25981 ataaaatcag aatagtcgct actaaagtaa tatcatcacg agaatagtta tatcctttag
    26041 taatagcaac agaacccaat cctcctgcac caatagcgcc agccatagct gtttcaccta
    26101 ctaaagaaat caaagtaacc gttgatactc gaattaaatc tggtagacct tcacgaagat
    26161 aaactacaat aatatcccaa agtgttccac ctgaggcttg tgcagcctca ataactccac
    26221 catcaagttc agctaaaaca acttgaactt gacgagcaaa aaatgggaaa actgccaaag
    26281 aaagaggtac caaagctgct ggtgaaccaa gtgttgttcc tacaattacg cgagttactg
    26341 gcgcaatcaa agcaagaaga ataatgaagg gcagagctct aaaaacagaa acaactttat
    26401 ctaaaactcc aaatactaat ttattagcaa taactcctct aggactagtt aaaacaagga
    26461 ataaacctcc taacaatccc attaaacctc caataaggaa cgaccaaaaa gtcatataaa
    26521 gggtttgtac aatagctgtc tgccagccgt aagcaccttc ccaacccatt tgatatacat
    26581 ttggtaaatg tgtttgaatc cactctatca tgctttacca ccttctttca ataacgttaa
    26641 ttgaacttgc gaatctgtaa ttgcttggca ggcttgtctt aatttttctt cgtcacctga
    26701 taagataact accatttctc caactggaat accatctaaa atttcaatat tcccatagag
    26761 gatgttagca gttacttcaa attctttata aatttgattt aaaagcggct catccgtgct
    26821 atgcccagcg tatttcagtt gaactaattt gctacctact ggtagatttt tgacaacttc
    26881 ttgttgctca atcttaagca ttgcctcatc aataccagta gctattttta taaaatcttg
    26941 tgtaagactc tctctaggat gtgaaaaaat gtctaaaact gatccttctt caatcaattt
    27001 accattttgc ataacagcaa cgcgattagc aatgtctttg acaatttgca tctcatgtgt
    27061 aatcaaaaca atcgtaagtc ccaatttttt attcagatct tggagaaggg caagtatttg
    27121 ttttgttgtt ttagggtcaa gtgcagaggt agactcatct gaaattaaga ttttaggatc
    27181 gttagccaat gcacgcgcaa tggcaacacg ctgtttttgc cctcctgata attgtgaagg
    27241 atagttttga gcacgatcgg ataatccaac aagttctaat aatttagcaa ccttagctgc
    27301 tttcgcctct tttgataaac ctgaatgttt aagagcaaaa gctacattct gctctgctgt
    27361 caactgtgac attaggttaa aatgttgaaa aatcattcca atttcacggc gctgttcgcg
    27421 taattgtgtg ctagtcagtg tcaccttgtt gtcgtaaata acttggtcat caatagtaat
    27481 tttacctgct gaaggttctt gtagcaaatt aataacacga acaagggtag attttccggc
    27541 tcctgaataa ccgacaattc catatatatc accttgatta atatggatag taacatcttt
    27601 aacagcatta atctcacgtt tcttttggtg gaaagtaaca tcaatgttgt ctaatttaat
    27661 aattggttct ttactcatat gattctatta aagcctccac taattcaata tgggtgtaat
    27721 aatcagcaat actaacattt tcatcaacgc catggtctcg actattggca tgtcctatgc
    27781 caatagctgc aattggcgct cttagagctt gatgaactaa atacattggt cctgtacctg
    27841 gtgaggtagg taacagagat atcccttctg gataaaattg ctcagctaaa tcaacaacct
    27901 ttaggataga tggcgctgac atatcactac gataagacat ttcaccaagg gtataggtta
    27961 gctcaatgtc tttaaagcca ttctctttca aatgattttg tattgaatct agtacacctt
    28021 tcggggttaa ccctggtact aacctaacct ctgctttaca tttagcataa gcaggtaaaa
    28081 ttgttttgac gccttcacct tgataacctg aggttatacc ttcaatggct atagaaggtt
    28141 caaaataaag ctttctcaaa aaagttttat gactatctgc taaactgggc aaaactaatt
    28201 ggtaagcacc ttctagcgcc tttgcagatc tataagaata tttttcaact aattctaact
    28261 ctctcttagt agggggaata actttatcat aaataccttc taccaatata tgacctttgt
    28321 tatcccttaa cgtattgagg gcttgaatta aataccatgt cgaggaatca acaaccccac
    28381 caaaggatga atgaatatca acatccgctg aacttactga taaatcaaaa gtgacaatgc
    28441 ctttatttcc accagcaatt tccaactgtc ctttaggatt tttagggcca tcttcccaca
    28501 cgatcaaatc agccccttgt agctgctctt gatacttttc taaataataa tcaaggccta
    28561 ctgaagctga ttcctctgct ccttccacaa taaaagtgat atctagagga agttcccctt
    28621 tgtgtcttga taaatatttt ttaactgctg ataaacgtgc ggtaatgtga cctttatcat
    28681 catctacacc acgtccatac atcttaccat atcgaagtga aagtgtaaaa gggtcctcag
    28741 tccactgttc tacctcatct gctggtacag tatcataatg attgtaaaaa ataatacgct
    28801 tagcatctac ttttgatgat ttgaaatttg ccacgataaa gggagcgcta tagctgtcat
    28861 ctataatgac ttctgcgcca gcttttatga aaacttctcc aagatagctt gataagtcat
    28921 ttaaaccaac tttctgagca aaaattgact taattgcaat taaatctctt aaaatattga
    28981 tatcatcctg aacaatagta tcagatagaa acttttgaat ttgattttct tcgtttgtta
    29041 aagtcatctt tcatccacct tccttcaaaa acaatccaag agcctggcac attgtattcg
    29101 tcccaggccc tttatggcta tatggctgat aagttgtatc ctattaggat tccctgttaa
    29161 acttatacag tcaattcacg ttaccattgt ggaatatcag ctgaagtatc tttgataact
    29221 tttttcactt catctgtgtg ataagcatcc aagatagctt ggatagcttt agcgttcttt
    29281 tgctttttcc aatttttacg tcccgcaatg atattaatcc attgttttga atttttatct
    29341 gatttctcaa caaagatagc atctgaaggt tttaaattag cttgctcaat gtatgtatta
    29401 ttaataatag ctgcatctac atctttgagt gcacgtggtg tttgactcgc atctaactcc
    29461 tgaatattaa tatccttttt attagatgtg atattagcaa ctgttgcaac cttcttacca
    29521 gaaacattca atttgattaa acctgctgac tgaaggacat acaatgcacg gctaccattt
    29581 gttgcatcat ttggaattgc aatagtggct ccttttttca attttttaag agattttacc
    29641 ttctcagaat agatacgaat tggagctaag taagtctttt caagtggaat taagtttttc
    29701 ttattttcct tattccagtt ttctaagaaa ttgtaatgtt gaaaggcatt aatatccaca
    29761 tccttattgg ctgtcgcttg atttggttgt gtataatctg taaattctgt aaatttgatt
    29821 ttagctttat cacctactag cttttcaatt ttatcccaac gtgctttttc agtgtcagaa
    29881 aaggtcataa caccaacttt aacaacttta cttgaagttg agcttgctga aacttcttga
    29941 ttactaaatg tgactcctaa aagtgttgtt aaggctaatc ctaagacaat atgttttaac
    30001 ttcataataa attccctcaa tttttgtgat aaagatatta tcccatagga agttattaga
    30061 atcaaattgt tataaactgt ctcttgttat agttttaaac tatataacaa aaaactaagg
    30121 attgacatct ccttagtttt tattactact tctccttagg agtaacgaga tctttctcat
    30181 ttggtacgta atcacctcca aggtattctt tagctatttt tgaaagtgtc ccatctttac
    30241 gtaattgttt aaggcgatta ttcacatact tttgcaagtc tttttgatcc tgaccaaaga
    30301 tgaaataaat ataaggctga tctctcatag tcaaagggat ggtttttaaa ttcgtgagac
    30361 cttgattctt tatgatagcg ttaacagttg gtccgtcaaa aagtttaaaa tcagcttttc
    30421 catcactcaa attcgttaga atctgtgtaa tattttcatt agtatatttt agagtaacag
    30481 gatttttctg atgctcttta ttgaaatttt ctaattgctt ggcagttgtc gtcccttgga
    30541 caacctgtgt tttatgacca ctaatatcat tataagcttt gatattacta ttcttaggaa
    30601 ctgctaaaac tgaaggagta gagcctattg ggtatgaaaa taaatatttt tgagatctct
    30661 ctgatgaata agaaatatta tttccaccca tttgaaactt tcctgaatca atgccggtaa
    30721 ataccgatga ccattctgtt tttttaaaag tgactttata gttatctgaa cctttaaaaa
    30781 cagctttggc tacttcaata tcaaagccag tcagtttccc atccttcaca taagaaaaag
    30841 gtgccgttgt tccaacagtt gcaaaattaa cttctttttt gcccgtctta gtagtatgag
    30901 attttgagct acaagcagct agagacgcta ctgtcactaa aataattcct gttacactta
    30961 acactttttt tattgtcatc ttttttctcc tctttaattg tacacgacat cataacatct
    31021 ttaagttttt aactaacact tatcttctat cgtctgagag tttttgctat tatatggatg
    31081 ctacgtttca ttaacaaaat aaccaaataa ggtttggttt tcaatttggt gaagcattaa
    31141 ttctggatgc cattgaacac ctataataca ttgttctgtt cgactttcat aagcttctac
    31201 tgtcccatct ctaggatctc tagcactaac tatcagacct tcagctaaca gtttaacact
    31261 ttgtctatga agagaattga tcttaaaatc acctgatata aagccttcta gaaaactccc
    31321 tttttccaca tttaaataat gagcaaatcc aatgtaaggc tcttgatagt gattatcaat
    31381 agattgatta agtgtaccac caagcgccac atttactaac tgcatgccac ggcaaatcgc
    31441 aaagattggt ttattttgtt tcaacgcttc ttcgactaat gcaaattcaa aaatatcacg
    31501 cgcaagtgaa taatcatcac tctcaattat cttctcttca ccataatagg atggtaaaac
    31561 attttggcca ccagatatga tcagcttatc tatcatctca acataagctt tggcactttc
    31621 tgcttcactt attggtagga taactggtaa cccgcctgcg tttttaactc cttccgaaat
    31681 atgcctagat accgaatcat aataataacc tggaatatct gacatttctc tttcatttcc
    31741 tgtaatacca ataataggct ttgttaacat tttgctccct catcaaccaa caactgtcta
    31801 ataattatca ttttacacct aaaaaaactt tttgaataat attttttacc tatcacttct
    31861 ttaacaatac ctatcaactc tcacaaaaat aaaaaccata gtattaatac tacggtttta
    31921 ttttttagaa atctgctaca ttatgataaa ctttttgaac atcatcatct gattcgagtg
    31981 cgtcaataag cttttcaaaa gtttcaaggt catcaccttc caatactact tctgattgag
    32041 gaatcatttc aagttcagta acttggaatt cttctacacc attatcgcga agtgcttgga
    32101 tacctttatg aagatctgtt ggggcggtat aaactgttat tgttccctct tctgcttcaa
    32161 catcatctac gtctacatcc gcttcaagta attgttcgaa gacagtgtca gcatcatcac
    32221 cagcaaaaac gatgacacct tttttatcaa ataagtagga taccgatcct gaagctccca
    32281 tattgccacc gttcttaccg taagcagtac gtacatttgc tgccgtacgg ttaacatttg
    32341 atgtcaaagt atccacaata atcattgaac catttggacc aaaaccttca tagcgtccct
    32401 ctacgaaagt ttcatctgtg tttcctttgg ctttatcaat cgctttatca ataacatgct
    32461 ttggaacttg tgcttgctta gcacggtcca aaacgaattt tagagctgag tttgactcgg
    32521 ggtctggttc accttgcttt gcagcaacat atatttcaac accgaattta gcgtatactt
    32581 ttgagttagc accatcttta gcagtctttt tggcaacaat attggcccac ttacgtccca
    32641 ttggaaaatc tcctttgatt tcatttttaa taacctgctg gctcatatta gcaagcaatc
    32701 ttaattttaa ctctattatt atagcacttt gatagcaatt ggtaaaatgg aaattctatc
    32761 accaagctct agcatctgtc ttatgtttta agtaatcaat ccatctacta tcctttacac
    32821 gacacaaggt aactgataaa ccagccatat ctaaacttgt aattaggtgg cctgttttaa
    32881 taaaaggtaa ttgtaaatca tctaaagcca gaaattccat gacagcattt gtaaaaagta
    32941 attcctccat tttagaactt cctccaagat tatttatcaa tagaatgaat gcttctccat
    33001 cttgccaacg taatttcatt ttcaatttgt taactagttc gtttgccaaa tgttccatgg
    33061 acacgaaagg cattgtccgg tatcctggct caccatgaat accgattcca aatgagatgt
    33121 aaccttcttc taaatcaaaa acaggttgat gctgacctaa aatagtggct gacttacttg
    33181 ctacacctaa agtagccatt gaagttgtca aagatagtcc tagctgttct aattcatcta
    33241 agcttgctcc ttctaaagca gcttgtccaa taattttatg tagcaacaca gtaccagcaa
    33301 cgcctcgatg acgaatttta aaatttgatg tttcaacaga gatgtcatca tgagaaacga
    33361 tgtatttgat tggaatgcct tcttgacgag cctgttcaat cgcttgggaa aattcttcca
    33421 aatcagcttc aaaatttttg ataataacaa agacaccttt gcctcgattg ataaatctaa
    33481 tagtctctag aatatcacta gcacatggtg gtacaaaaat aggaccacta atcgctgccg
    33541 atagcattcc ctcaccaaca tatccaaagt gagccggctc atgtccgctg cctcctcctg
    33601 ataaaatcgg aattacttgc tcggattgcc ctaaataatg aatagcgggc aaatctttta
    33661 tttgctctac tttaggatta gttttagtga ttgcttttat tgtttcaaaa acatattcca
    33721 ttttatgatg ttctgttttt ctcatatcct tgtcctaata attttctatt gatgttctga
    33781 taattttttc aatgactttc ctattaaata attcttcttt tgagttattt attaactc
//
LOCUS       DATRSD010000010        32762 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRSD010000010 DATRSD010000000
VERSION     DATRSD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32762
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.010"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            291..1331
                     /gene="pheS"
                     /locus_tag="VBD85_000399"
     CDS             291..1331
                     /gene="pheS"
                     /locus_tag="VBD85_000399"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO7132974.1"
                     /translation="MDLQKQLEKLKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            1414..1935
                     /locus_tag="VBD85_000400"
     CDS             1414..1935
                     /locus_tag="VBD85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7132975.1"
                     /translation="MSQITFKKVGLDNVNILQNIAIETFRQTFSHDNSEEQLQAFFNE
                     SYTLPVLKSEITHAESDTYFVYLDTDLVGYLKVNWGSQQTEKDLDKAFEIQRIYLLDA
                     YQGQGIGKATFEFALDLAYKSGLDWAWLGVWEFNHKAQAFYAKYGFEKFSEHQFSVGD
                     KVDTDWLLRKSLH"
     gene            1989..4394
                     /locus_tag="VBD85_000401"
     CDS             1989..4394
                     /locus_tag="VBD85_000401"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO7132976.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTYLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIGSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGG
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GAGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVSAIETSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(4463..5365)
                     /locus_tag="VBD85_000402"
     CDS             complement(4463..5365)
                     /locus_tag="VBD85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO7132977.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLLRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASGPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAEQR
                     QRWFNKGFQYGDIQHGDTFSVEHL"
     gene            5543..8776
                     /gene="rexB"
                     /locus_tag="VBD85_000403"
     CDS             5543..8776
                     /gene="rexB"
                     /locus_tag="VBD85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO7132978.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLENLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILELKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKANFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEISQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTQENLKKNHFAALSLFNAAKQE
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DDPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSETEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            8766..12389
                     /gene="addA"
                     /locus_tag="VBD85_000404"
     CDS             8766..12389
                     /gene="addA"
                     /locus_tag="VBD85_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO7132979.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIDSLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSTDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLTNLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQLYQDKYHEVMVDEYQDNNHTQERMLELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNHLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKVENRQ
                     SEDIVRAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSVKAA
                     IQIEKINYFFQETSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIEKIDKYLILLGGKQLEVV
                     KMD"
     gene            12402..13319
                     /locus_tag="VBD85_000405"
     CDS             12402..13319
                     /locus_tag="VBD85_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7132980.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(13352..14728)
                     /gene="mnmE"
                     /locus_tag="VBD85_000406"
     CDS             complement(13352..14728)
                     /gene="mnmE"
                     /locus_tag="VBD85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO7132981.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPKNVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDVAPDELITQL
                     FSQFCLGK"
     gene            14856..16766
                     /locus_tag="VBD85_000407"
     CDS             14856..16766
                     /locus_tag="VBD85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7132982.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWDAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVVEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            16916..17884
                     /locus_tag="VBD85_000408"
     CDS             16916..17884
                     /locus_tag="VBD85_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO7132983.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            17959..18957
                     /locus_tag="VBD85_000409"
     CDS             17959..18957
                     /locus_tag="VBD85_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO7132984.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            19084..20472
                     /locus_tag="VBD85_000410"
     CDS             19084..20472
                     /locus_tag="VBD85_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO7132985.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGYIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     NFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVETKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            20532..22289
                     /gene="lpdA"
                     /locus_tag="VBD85_000411"
     CDS             20532..22289
                     /gene="lpdA"
                     /locus_tag="VBD85_000411"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO7132986.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            22387..23376
                     /locus_tag="VBD85_000412"
     CDS             22387..23376
                     /locus_tag="VBD85_000412"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7132987.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQMKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
     gene            complement(23484..24269)
                     /locus_tag="VBD85_000413"
     CDS             complement(23484..24269)
                     /locus_tag="VBD85_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO7132988.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(24269..25612)
                     /locus_tag="VBD85_000414"
     CDS             complement(24269..25612)
                     /locus_tag="VBD85_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO7132989.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFNTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLTHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEPSQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMTMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            25752..26603
                     /locus_tag="VBD85_000415"
     CDS             25752..26603
                     /locus_tag="VBD85_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO7132990.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            26606..27565
                     /locus_tag="VBD85_000416"
     CDS             26606..27565
                     /locus_tag="VBD85_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO7132991.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            27619..28971
                     /locus_tag="VBD85_000417"
     CDS             27619..28971
                     /locus_tag="VBD85_000417"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO7132992.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMKVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            29094..29465
                     /locus_tag="VBD85_000418"
     CDS             29094..29465
                     /locus_tag="VBD85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO7132993.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            29490..29870
                     /locus_tag="VBD85_000419"
     CDS             29490..29870
                     /locus_tag="VBD85_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7132994.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            29962..31092
                     /locus_tag="VBD85_000420"
     CDS             29962..31092
                     /locus_tag="VBD85_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO7132995.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEAQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKEGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            31096..31833
                     /locus_tag="VBD85_000421"
     CDS             31096..31833
                     /locus_tag="VBD85_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO7132996.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            31834..32604
                     /locus_tag="VBD85_000422"
     CDS             31834..32604
                     /locus_tag="VBD85_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO7132997.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            32594..>32762
                     /locus_tag="VBD85_000423"
     CDS             32594..>32762
                     /locus_tag="VBD85_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEO7132998.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNK"
BASE COUNT        10826 a         5199 c         6532 g        10205 t
ORIGIN      
        1 ctttatctaa tagtgcttgt taaagactga aaaatttgat aaaatagtct ttaattgaat
       61 agaggtctgt gagactagta aaccaaggga agctgcttag ggagttcagc cgtgactgca
      121 agctgaatca gtgaaagagg gtggaattca ctcacaaata ggactataat taaggtctga
      181 ttggtcgatc agatagaaaa cggatggtac cgcgtgtcaa cgctccgatt aaggggtagt
      241 ggcacgcttt tttttgtttt caaagctact tattttgact tggaggaaag atggatttac
      301 aaaaacaatt agaaaagttg aaaatatcaa ctcaagaaaa gcttaaagaa atgactggga
      361 accacactaa agaattacaa gacttacgtg tacaagtttt aggaaaaaaa ggttctctaa
      421 cagaattact aaaaggtctg aaagatttat ctaatgattt acgaccagtt gttggaaaac
      481 aagtcaatga agtacgtgat attttaacaa aagcgtttga ggaacaagct aaagtagtgg
      541 aagctgcgaa gattcaagca cagttagagt ctgagagtgt cgacgttacc ttgcctggtc
      601 gtcaaatgac attaggtcac cgtcatgtct taacacaaac tagcgaagag attgaagata
      661 tctttttagg tatgggattc caagtggttg atggttttga agttgagaaa gactactata
      721 attttgaacg aatgaattta ccaaaagatc atccagcccg tgatatgcaa gatacttttt
      781 atattacaga agaaatcttg ctacgtactc atacttcgcc tgttcaagca cgaacaatgg
      841 accaacatga tttttctaaa gggcctttaa aaatgatttc tcctggacgt gttttccgtc
      901 gtgatactga tgatgctact catagccatc aattccatca aattgaaggc ttagttgttg
      961 gagaaaatat ttcaatgggc gatctcaaag gaacacttca actgatttct caaaaaatgt
     1021 ttggagcaga gcgtaaaatc cgacttcgtc cttcatattt cccattcaca gaaccttcag
     1081 ttgaagttga tgtctcttgc ttcaagtgcg gaggtaaggg atgtaatgtc tgcaaacaga
     1141 caggttggat tgagatattg ggtgctggta tggttcaccc aagtgttttg gaaatgtctg
     1201 gtattgattc tgagaaatac tccggttttg cctttggttt aggtcaagaa cgtattgcaa
     1261 tgttacgcta cggtatcaat gacattcgtg gcttctatca aggtgatgtt cgcttcacag
     1321 atcaatttta agattttaga tagcaagtag catcattggt gtgatgataa atttatgtta
     1381 acgctattac cttactcaat aggaggtttt ctaatgtctc aaattacttt taaaaaggtt
     1441 ggccttgata atgtgaatat tttacagaat attgctatag aaacctttcg gcagacgttt
     1501 tctcatgata atagtgagga acaacttcaa gcatttttca atgagtctta cactttacca
     1561 gtattaaaat cggaaattac ccatgctgaa tctgatactt attttgttta tcttgataca
     1621 gatttagtgg gttatttaaa agtaaactgg ggaagtcagc aaactgaaaa agatcttgat
     1681 aaagcatttg aaattcaaag gatttatctt ctagatgcct accaagggca aggtataggt
     1741 aaggctactt tcgaatttgc tttggattta gcttataaat caggcttaga ttgggcttgg
     1801 ttaggtgttt gggagtttaa tcataaagct caggcatttt acgctaaata cggatttgaa
     1861 aaattttctg agcaccaatt ttcagtaggt gataaagtgg atacggattg gttattaaga
     1921 aaaagtttac actaaaaact acaaagcact tgtataaaaa taaaagaaaa taaatagaga
     1981 gaaattacat gttagtatcg tataaatggt taaaagagtt agtggatgtt gatgttacaa
     2041 cagcagaact agctgagaaa atgtcaacca ctggtattga ggttgaaggt gtggagacac
     2101 cagcggaagg tttatcaaaa ttagttgtag gtcatattgt ttcgtgtgaa gatgttccag
     2161 atacctactt acatttgtgt caagttgata caggggatga tgaacttcgt caagttgtct
     2221 gtggtgctcc aaatgtcaag actggtatta atgttatcgt agcggttcct ggtgctcgta
     2281 ttgcagataa ctataagatt aaaaaaggta aaatccgtgg tatggagtca cttggaatga
     2341 tttgttcact tcaagagttg ggcttatcgg aaagtatcat tccaaaagag ttttctgatg
     2401 gtatccaaat tttacctgag ggagctatac ctggtgatag tattttctct tatcttgact
     2461 tagatgatga aattattgaa ttatcaatta caccaaaccg tgcagatgct ctttcaatgc
     2521 gtggtgttgc ccatgaggta gcagcaattt atgggaaaaa agttcatttt gaagaaaaaa
     2581 atctcattga agaagcagaa agagcagctg ataagatttc tgttgtcatt gaatcagata
     2641 aagttttatc ttactcggct cgtattgtta agaatgtaac agttgcacca agtccacaat
     2701 ggcttcaaaa taaactgatg aatgctggta tacgtccaat taataatgtt gttgatgtga
     2761 caaactatgt tcttttgact tatggtcaac caatgcatgc ttttgatttt gataaatttg
     2821 atgggacaac tatagtagca cgtaatgctg agaatgggga aaagttaatt actcttgatg
     2881 gtgaagagcg tgacttaata gctgatgact tggtcattgc ggtaaatgat caaccagttg
     2941 ctttggcagg cgttatggga ggacaatcaa ctgaaattgg tagttcttct aagacagtgg
     3001 tccttgaagc ggcagtattt aatggaacaa gtatccgcaa aacaagtgga agactcaacc
     3061 ttcgttcaga atcatcttct cgctttgaaa aaggtatcaa ttatgataca gtttcagagg
     3121 ctatggattt tgcggctgca atgttacaag aattggcagg tggtcaagtt ctttctggtc
     3181 aagttacaga aggagttctt ccaactgagc cggtagaggt atcaacgact cttggttacg
     3241 ttaatactcg tttgggaaca gaacttacct atactgatat tgaagaagta tttgaaaaac
     3301 tcggctttgc tatttctggt tcagaagtga aatttactgt gttggtaccg cgtcgtagat
     3361 gggatattgc cattcaagct gacttagttg aagagattgc ccgtatttat ggttacgaaa
     3421 aattaccaac aacattgcca gaagccggag cgactgcagg agaattaacg agtatgcaac
     3481 gcttacgtcg tagagtccgt acggttgctg aaggagcagg attatctgaa attatcacct
     3541 atgctttaac aactccagaa aaagcagtgc agttctcaac tcaagcaact aatataactg
     3601 aacttatgtg gccgatgact gttgatcgct cagcacttcg ccaaaatgtt gtttcaggaa
     3661 tgcttgatac aattgcttat aacgttgctc gcaaaaacag taaccttgca gtttatgaga
     3721 taggaaaagt ttttgaacaa actggtaatc caaaagaaga tttaccaact gaagtagaga
     3781 cgtttacttt tgccttaaca ggtctagtag aggaaaaaga tttccaaaca aaagccaagc
     3841 cagttgattt cttctacgcc aaaggtattg tagaggcgct ctttattaaa ttaaaattag
     3901 atgttacttt tgtagcacaa aaaggtttag caagtatgca cccaggtcgt acagcaacta
     3961 tcttattaga tgggaaagag attggttttg ttggtcaagt acacccacaa actgcaaaac
     4021 aatatgatat tcctgaaact tatgttgctg aaatcaatct atcaactatt gaaagtcaaa
     4081 tgaaccaagc gttgattttt gaagatatta cgaagtatcc gtctgtatct cgcgatattg
     4141 cattattact tgcagaatct gttagtcatc atgatattgt gtcagcaatt gagacatcag
     4201 gggtaaaacg tctaacagcg attaaactgt ttgatgtcta cgctggaaat aatattgcag
     4261 aagggtataa atcaatggct tatagcttaa ctttccaaaa ccctaatgac aatctaacgg
     4321 acgaagaagt ggctaaatat atggaaaaaa tcactaaatc actcgttgag aaagttaatg
     4381 cagaaataag ataatttata ttttacgttg aaaaataaat aaaaaacagc atcaattaag
     4441 atgctgtttt tttatttaaa atttatagat gttctacgga gaaagtatca ccgtgttgga
     4501 tgtcaccata ttgaaagcct ttgttaaacc aacgttggcg ttgttcagct gttccatggg
     4561 taaagctatc aggcactaat tttccgtagg tttctttctg aagggtatcg tctccgacgg
     4621 cgtgggcagc attcatggcc tcttcaaagt ctccttgttc taagagattt tttcccctga
     4681 tgtagtgagc ccatacccct gcataataat ctgcttgaag ttctagccga acatttaaag
     4741 catttgcttc tttcttagta agtccgtgtc gcattctatt atacttatcc ataatgccta
     4801 actctgtttg aatgtggtga ccaacttcgt gggcgatgac gtaggccata gcaaaatcac
     4861 cagtagcacc atatttatgt gataattcat tgtaaaaaga aatatcaaga tagatttttt
     4921 tatctgctga acaataaaat ggtcctgaag cagattcacc tataccacaa cctgtttgaa
     4981 ttgaattggt gtaaagaaca agttttggtt ccttataatt tccaaaacct tgggtttgga
     5041 attcttgtga ccagaaatcc tcagttgagc caaggacttt attaacgaaa tcgatttgtt
     5101 ctctcgttgc gctattatca acagaacgtg agacattctg agattggtaa ctagaaggtg
     5161 aggatgagtc attaaaaatg ctggttagtc cccctccgcc aagtagcagt aagatgatta
     5221 aaaccacaag cttggttttc caactccctc gcagcaataa aagttgaaga atcggtaatc
     5281 cacttcctcc gctagagaat gaacctccgc tactggagcg acgatcttca acattgtcgc
     5341 tttttcttag gtcatctatt ttcataagta tctccaaagt gttttatagg ctcattataa
     5401 actattttaa taagaaatgc ttgaattttt aactaaatat cttcccctag atttgaagct
     5461 atttttggta taatattaat aaactgtaaa ggataagttg aagaaggttt acttctcagt
     5521 tgacttataa gataggagat tcatgaaatt actttatact gatattaatc atgatatgac
     5581 agaaatctta gtcaatcagg ccgcgcatgc tgctgaagct ggatggcgta ttttttatat
     5641 tgctccaaac tctttatctt ttgaaaaaga gcgtgcagta ttagagaacc ttcctcaaga
     5701 agcctctttt gctattacaa ttactcgctt tgctcaattg gcacgttatt ttactttaaa
     5761 ccagcctaat caaaaagaaa gtttaaacga tattggatta gcaatgatct tttatcgtgc
     5821 tttagcaagt tttgaagatg gacaattaaa agtttttgga cgattgaagc aagatgcttc
     5881 ctttatcagt caattggtag acctatacaa agaattgcag acagcaaatc tgagtatttt
     5941 agagttaaaa tatttacatt ctcctgaaaa atttgaagat ttattagcta tttttctagt
     6001 ggtttcagat cttttaagag aaggagaata cgacaatcaa tctaaaattg ccttttttac
     6061 agaacaagta aggtcaggcc agctagatgt ggatttaaag aacactatcc ttatagtcga
     6121 tggttttacc cgtttctcag ctgaagaaga agctttaatt aaaagtctga gttcacgttg
     6181 tcaagagatt atcatcggag cttatgctag ccaaaaagcg tataaggcta actttactaa
     6241 tggaaatatt tattctgcag gggttgattt tttaagatat ctagcaacga catttcaaac
     6301 aaaaccagaa tttatcttgt ccaagtggga aagtaaatct ggttttgaaa tgatttcgaa
     6361 aaatatcgaa ggtaaacatg attttacaaa tagtagtcac attttagacg acactgctaa
     6421 agattgtata acaatttggg agtgcattaa tcaaaaagat gaagtggaac atgtggctag
     6481 agctatacgt cagaaacttt atcaaggata tcgctacaaa gatattttag ttttactagg
     6541 agatgtagat agttataaac tacaattaag taagattttt gagcagtatg atattcctta
     6601 ttatttcggt aaagctgaaa caatggctgc gcacccttta gttcatttta tggattcctt
     6661 gtctcgaata aaacgttatc gatttagagc tgaagatgtt ttaaatcttt ttaagacagg
     6721 tatttatgga gagattagcc aagatgattt agattatttt gaggcttata tctcatatgc
     6781 agacattaaa ggtcctaaga agttctttac agattttgtt gtgggggcta aaaaatttga
     6841 tttagggcgt cttaacacta ttcgtcagtc cctccttact ccactggaaa gttttgtgaa
     6901 aacgaaaaaa caagacggta taaaaacact caaccaattc atgtttttcc ttactcaggt
     6961 aggtttgagt gacaatttat cgcgattagt tggtcaaatg tctgaaaatg aacaagaaaa
     7021 acatcaagaa gtttggaaaa catttacaga tattttagag cagtttcaaa caatatttgg
     7081 tcaagaaaaa ttaaatttag acgaattttt atcgttatta aattcaggta tgatgcaagc
     7141 cgagtatcgt atggtccctg ctactgttga tgtggttact gttaagtctt acgatttggt
     7201 agaaccacat agtaatcaat ttgtctatgc cctaggcatg acacaatcac attttccaaa
     7261 gattgctcaa aacaaatctt taatatctga tattgaacgt cagcttataa atgatgccaa
     7321 tgacactgat ggtcattttg acattatgac gcaagaaaat ttgaagaaaa accattttgc
     7381 tgccctctct ctttttaatg ctgctaaaca agaattagtg ttgactatac cacaattatt
     7441 gaatgagtct gaagatcaga tgtcaccata tcttgtcgaa ctaagagata taggtgttcc
     7501 ttttaatcat aaaggacgcc aatcgttaaa agaagaagct gataatattg ggaattacaa
     7561 agctttatta tcgcgtgttg ttgacttgta tcgctctgct attgacaaag agatgactaa
     7621 agaagaacaa actttttggt cagttgctgt tcgttatttg cgtcgtcaat taacttctaa
     7681 agggattgag ataccaataa ttacagatag tcttgatact gtgacagttt catcagatgt
     7741 gatgaccaga cgcttcccag aagatgaccc acttaaatta tcctcatctg ctttgacaac
     7801 tttttataat aatcaatata agtatttttt acaatatgtg ttaggtttgg aagaacaaga
     7861 ctcaattcat ccagatatgc ggcaccatgg aacgtacctt catcgtgttt ttgaaattct
     7921 gatgaaaaac caagggatag aatcttttga agagaaactc aactctgcaa ttaataaaac
     7981 caatcaagaa gatgttttca aatccttgta ttcagaagat gcagaaagtc gttattcttt
     8041 agaaatttta gaagatatcg cgcgtgcaac tgcaaccata ttacgacaag atagtcagat
     8101 gactgttgaa agtgaagaag aacgatttga actaatgatt gataatacta taaaaattaa
     8161 cggtattatt gaccgtatcg atcgtttatc tgatggttct ttgggtgtcg tggactataa
     8221 atcaagcgct caaaaattcg atatccaaaa attttataac ggtctaagtc cacaattagt
     8281 cacctacatt gatgctatca gtcgtgacaa ggaagttgaa caaaaaccac cgatttttgg
     8341 agctatgtac ttacatatgc aggaacctag acaagacttg tctaaaatta aaaatttaga
     8401 tgatttagta acaaaaaatc atcaagcttt aacttataaa ggattatttt cagaagccga
     8461 aaaagaattc ttagcaaatg ggaaatacca tctcaaagat tccctttatt cagagactga
     8521 aattgctatt ttacaagcac ataaccaatc gctttacaaa aaagcatctg agactattaa
     8581 gtcaggtaag tttttgatca atccatatac agaagatgct aaaacagttg acggagatca
     8641 gtttaagagt attacgggat tcgaggcgga taggcatatg gcacgtgcca gagcgcttta
     8701 taaattacct gctaaggaaa aacgtcaagg tttcttaaca ttaatgcagc aagaggagga
     8761 aaatgatgac ctttaagcct ttcttaaatc cagaagatat tgctgttatt cagacagaag
     8821 aaaaaaattc tgataaaaaa caaaagcgaa cgcctgaaca aattgaagcc atttatactt
     8881 ttggtaacaa tgtcctggta tcagcttcgg ctggttctgg aaagactttt gtaatggttg
     8941 agcggatttt agataaactt ttaagagggg ttccgattga ctctttgttt atctcaacat
     9001 ttacagtaaa agcggcaggt gagctcaaag aacgtttaga aaagaaaata aacgagtctt
     9061 tgaaatccgc agaatcagat gatttaaaac agtttctcac ccagcaactt gtcggcattc
     9121 aaacagctga tattggtact atggatgctt ttacgcaaaa aatagttaac caatatggtt
     9181 atactttggg catatcacct atatttagga ttttacaaga taaaaatgag caagacgtca
     9241 taaagaatga ggtctatgct gatttatttt ctgactacat gactgggaag aatgcagctt
     9301 cattcataaa attagtcaaa aatttctcag gtaatcgtaa agatagcaaa gcttttcgag
     9361 aaatggttta taaagtttat gcttttagcc agtccaccga taaccccaag cgctggatgc
     9421 aaacagtttt tctaaaaggt gcacaaactt acactgattt tgaagctatt ccagatcaag
     9481 aagtttcaag ccttttaaat gtaatgcaaa caactgctaa tcaattacgt gatttaacgg
     9541 atcaagaaga ttataagcaa ttaactgcta aaggggttcc gacagcaaat tacaaaaaac
     9601 accttaaaat aattgaaaat ttagtccatt ggtctcaaga ctttaatttg ctgtatggaa
     9661 aaaaaggatt gactaatttg gcacgtgata tcactaatgt gattccttca ggcaatgatg
     9721 ttacagttgc tggcgttaaa tatcctatct ttaaacaatt gcataatcgt atcgttggac
     9781 ttaagcactt agaagttatt tttaagtatc aaggagaatc tcttttcttg ttagaattat
     9841 tgcaatcctt tgttttagat ttctcagaac aatatttaca ggagaagatt caagaaaatg
     9901 cttttgaatt ttctgatatt gcccattttg caattcaaat attggaagaa aatcatgata
     9961 ttaggcaact ttatcaagat aagtatcatg aagtaatggt tgatgagtat caggacaata
    10021 accataccca agagagaatg ttagaattat tgtctaatgg acataatcgt ttcatggttg
    10081 gagacataaa acaatcaatt taccgttttc gtcaggctga tccacaaatt tttaatgata
    10141 aatacaaagc ttaccaagac aatccaagtc aagggaaatt aattattctg aaagagaatt
    10201 ttcggagtca atcagaggtt ttagacagta caaatagtgt gtttacacat cttatggatg
    10261 aagaagtagg agacattctt tatgatgaaa gtcaccaatt aaaagctggt agtccaagac
    10321 agcaagaaag acatccgaat aataaaacac aagttttact attagatact gatgaagatg
    10381 atatagatga cagtgatagt caacaatatg atattagccc agctgaagca aagttagtcg
    10441 caaaagaaat tattcgattg cataaagaag agaatgttcc ctttcaagat atcaccctct
    10501 tagtttcttc aaggacaagg aatgatggta tcttacaaac ttttgaccgt tatggtattc
    10561 ctttggtaac tgacggtggg gagcaaaatt acttaaaatc agtcgaagtg atggtaatgc
    10621 ttgatacttt gcggtcaatt gataatccct taaatgatta tgctctagtt gctcttttga
    10681 gatctcccat gtttggcttt aatgaagatg atttaacaag aattgctatt caggatgtca
    10741 aaatggcttt ttatcataaa gtgaaattgt cttatcataa agaaggtcat catagtgatt
    10801 taattactcc agaattatct tctaaaatag atcactttat gaaaacattc cagacttggc
    10861 gtgattttgc taaatggcat tcgctttatg atttaatatg gaaaatatat aatgaccgtt
    10921 tttattatga ttatgtagga gctctgccta aagctgagca acgtcaagcc aatctatatg
    10981 ccttggcact tcgagcaaat cagtttgaaa aaacaggttt caagggttta tcacgcttta
    11041 ttcgaatgat tgataaagtt ttagaaaatg aaaatgattt ggcagatgtt gaggtggcat
    11101 taccgcaaaa tgctgttaac ctaatgacta tacacaaatc aaaggggctt gagtttaagt
    11161 atgtatttat tttaaatatt gataagaaat tttcaatggt agacataact tcccctctta
    11221 ttttaagccg aaatcaagga attggtatta aatatgttgc tgatatgcga catgaactcg
    11281 aagaagagat actgcctgca gtaaaggtta gcatggaaac acttccttac caacttaata
    11341 aaagagagtt acgcttagct actttatcag agcaaatgcg tttactttac gtagctatga
    11401 cacgtgcaga gaaaaaatta tatctggttg gtaaggctag tcaaaccaaa tgggctgatc
    11461 attatgactt agtatctgaa aataatcatc tcccattagc tagtcgtgag acttttgtga
    11521 cctttcaaga ttggttactg gctgttcatg aaacctataa gaaacaagag ttgttttatg
    11581 acatcaactt cgtatcatta gaggaattaa cagatcacca catagggatg gttaacccta
    11641 gtctaccttt caatcccgat aataaggtag agaatcgtca gtctgaagat atcgtgcgtg
    11701 cgattagcgt gttagaatct gttgagcaga ttaatcaaac ttataaagcg gctattgagt
    11761 taccgacagt gagaacacca agtcaagtca aaaaattcta tgagcctatt ctagatatag
    11821 aaggtgttga tgtgatggaa actattacta aaacatcagt agattttaag ttacctgatt
    11881 tttctacaag taaaaagcaa gacccagcag ctttaggatc tgctgtacat gaattaatgc
    11941 aacgcataga gatgtcatct catgtcaaaa tggaagatat tcaaaaagct ttgacagaag
    12001 tgaatgctga aacaagtgtc aaagcggcta ttcagattga aaaaatcaac tattttttcc
    12061 aagaaacctc actcggtaag tatatacaag aagaagtaga acatctgcat cgagaggcac
    12121 cttttgccat gttaaaagaa gatccggaga gtggtgagaa attcgttgta cgtggtatta
    12181 ttgatgggta tctactatta gaaaatcgga taattctatt tgattataag actgataaat
    12241 ttgtcaatcc tctagaatta aaagaacgct atcagggaca aatggcttta tatgcagaag
    12301 ctttaaaaaa atcttacgag atagaaaaga tagacaaata cttaattctt ttaggtggta
    12361 aacagctaga agtggtaaaa atggattaaa gaaaggaaaa aatgatagtc gaacaaaaat
    12421 ttggaaatgg ttttacttgg attaacattg aagcagagca attgcgaaca gaaacatcgg
    12481 aaattcaagc aaaatatttg gatagtgaga ttattacata tgctctggat gattatgaac
    12541 gagcctttat ggaatgttct catattaaag gaaaagaagt cttgacgatt atttttaata
    12601 ctattgatct aaagcagaaa gaaagttatt atgagaccgt tccgatgacc ttttgcttat
    12661 cacacgaccg tttgataacg gtgacacggt ctcgaaatag ttatatgcta gaactattgc
    12721 aaaaatatct agaccgtaat ccagatgtct ctcctaaaaa atttttattt gcagctttaa
    12781 ctttgattac aaaacaatat tttaatgttg tatcgaaaat tgatagagaa aaggatattc
    12841 tcaaccgtca attgagagaa caaacaacca ataagcgctt attagccatg agtgacctag
    12901 agacaggatc cgtttatctt ttgacagctg ccaatcaaaa tgcattggta ttagagcaat
    12961 tagatgttca tcctagtcag cgatttaatt ctgaagtgga aaaagaacag ttatcagatg
    13021 ctctcattga agcgcatcag ttagtcagta tgacacaatt aaacagtcag gtactttcac
    13081 aattatcatc tacttttaat aatgtcttaa ataataactt gaacgaaaac ttaacaggtt
    13141 tgaatatcat ttcgattaat ttagcgatta ttgcagcaat tactggcttt tttgggatga
    13201 atattccatt acctcttaca gaatcacgga gttcatggtt aattgttatt gctacttcgg
    13261 tcttactttg ggttattatt gctcaaattt taaaacgagt ttttaactta agaaaataaa
    13321 aatattcggt gcttacgcac cgaatatttt tttatttacc taagcagaat tggctgaaga
    13381 gctgagttat tagttcatct ggagctacat ccccagtgat ttctccaagt atttcccaag
    13441 ttctagtcat atcgacttgt aagagatcaa caggcattcc taattccaag ccctcattga
    13501 cagcttttaa actatcaacg gctttttcaa ttagtgagat atggcgagca tttgatagat
    13561 aagtagcgtc atgttctacc ataccagcgt tgtcaaagaa aatatcgtta atacgttctt
    13621 cgattttatc gatgttttca ttttctaaaa ccgaaattgg aataacattt ttaggtaatt
    13681 cgttaacttc tattttttga ggaagatccg ttttattgag taatacgata cgattacttt
    13741 ctttacttag ttctagaagg gagcggtctt gtaatgttag tggttcggaa ctattgagaa
    13801 ccaacaacac aaggtcggct tcttctaaag ccttctttga acgttcaaca ccaatttttt
    13861 caacgatatc atctgtatca cggataccag cagtgtcaac tagttttaaa ggaactcctt
    13921 taatattgac gtattcttca ataacatcac gagttgttcc ttcaatatct gtcacaatcg
    13981 ccttttcttc acgtaagagg ttatttagta agctggattt accgacattt ggacgaccaa
    14041 tgattgctgt tgaaagtcct tctcgaagaa tttttccacg tctggcagtt cgaagtaaat
    14101 tttccataag tgcttgaaac tcttgtgttt tttcacgcat cagtgttgta gtcatctctt
    14161 caacgtcatc atattcaggg taatcgatat taacttcaac ttgggctaga gtatttaaaa
    14221 tttcttgtcg agtgttattg attagtgtct ttaaagaacc atctaattgc ttaacggcaa
    14281 tatccattgc tttatctgtt ttagctctaa taagatccat aacagcttca gcctgagtca
    14341 agtcaactcg accatttaaa aaagcacgtt tagtaaattc tcctggttca gccattcgtg
    14401 ctccatgtcg taaaattaac tgaagaattt cgttagtaac tgcaattcct ccatgcgtat
    14461 taatttcaat aacatcttct cttgtgaatg tttttggagc aagcataacg cctaacataa
    14521 cttcatccaa gatttcattt ttatctgggt ccacaatatg accatagttt aaggtatggg
    14581 attggatcgc ggatagatct tttccacgat aaatttttga tgctatcttt aaggcatctg
    14641 taccagatat acggacaata ccaatagctc cctcaccgag aggagttgaa atagctgcga
    14701 tggtatcaaa ttcttttgta atagacataa agtctctctc tttcttggaa attactaaca
    14761 ttcattgtaa cctaaagccc taaaaggtgc aatcatttga ttaaaagaga agatttccat
    14821 aatggtttta tttgaaggta tgctataata aaactatgat tatactacaa ggaaacaaaa
    14881 ttgagcgctc attttcaggt gatgtgcttt ttgataatat taatattcaa gttgatcaac
    14941 gtgatcgtat tgctcttgtt ggacgtaatg gtgctggcaa atcaacgctt cttaaaattt
    15001 tagttggtga agaggctccg acaaaaggag aaataaataa aaagcgtgat ttgagtttgt
    15061 cctacttggc acaagatagt cgttttcaat ctgaaaatac aattttccaa gaaatgttac
    15121 aagtttttga ctcattaaga gaagtagaga agcgcttacg tgaattggaa ttacaaatgg
    15181 ggcaagtttc aggaagtgac ctggaacaat tgatgaaaac atacgatatt ttgtcagaag
    15241 agtttcgtga aaaaggtggc tttacctatg aatcagatat aaaggctatt ttaaatggtt
    15301 ttaagtttaa cagtgatatg tgggaaatgc ctattagtga attgtctggc gggcaaaata
    15361 cacgattagc gcttgcaaaa atgctacttg aaaagccaga attacttgtt ttagatgaac
    15421 caacgaacca tttagacatt gatacgattg cttggttaga aaattatctt gtaaattatc
    15481 aaggagccct tattattgtc agccatgacc gctatttttt agacaaggta gctacagtaa
    15541 cttatgattt aacgacacat tctctagacc gctatgtagg caattattcg aaatttatgg
    15601 atcttaaggc agagaaaata gcgactgaag aaaagaattt tgaaaaacaa caaaaagaaa
    15661 ttgctaagtt agaagatttt gtacaacgta acattgttag ggcttcaacc actaaacgag
    15721 cacaagctcg tcgtaaacaa ttagaaaaga tggaacgact cgacaaaccg aatgtagagc
    15781 aaaaatcagc taatatgact tttcatgctg ggaaagtctc agggaatgtt gttttaacac
    15841 ttgagaatgc agctattggt tatgaaggtg tttctttatc ggagccaatc gatttggacg
    15901 tgaagaaatt tgatgctatt gcaattgtag gtccaaatgg tatcgggaaa tcaactttaa
    15961 tcaaatcgct tgttgggcaa attcctttta tcaaaggaga agcaaaatta ggagcaaatg
    16021 tcgaaactgg gtattacgat caaagtcaaa gtaatttaac aaaaacgaat actgttttag
    16081 atgagttatg ggatgctttt tcaacaacgc cagaagttga aattagaaac cgtttaggag
    16141 cttttctttt ttcaggtgat gatgttaaaa aatcagtttc aatgctatca ggtggtgaac
    16201 gagcgcgttt gctattggca aaattatcta tggaaaataa taattttctc atattagatg
    16261 agccaacaaa ccacttggat attgatagta aagaagtctt agaaaatgca ctcatcgaat
    16321 ttgatggcac attacttttt gttagtcatg atcgctattt tattaatcgt gtggcaacta
    16381 aggttctcga aatttctgat aagggaagta ctctctatct aggagattat gattattacc
    16441 tcactaagaa agccgaacta gaggaattag cacgtttaaa tgaagaagaa gtgagtgctt
    16501 caaaaacaga aatcgacgtt acatcagatt atgagacaca aaaagcaaat caaaaggaat
    16561 ttaggaaaat tacccgtcgc gttgtagaaa ttgaggcgcg tttggaagtt cttgaaaatg
    16621 atgaaaacaa tatcaatggt ttaatgttag aaaccaatga catcggaaaa ctctctgatt
    16681 tgcaaaaaga gctggaatca atccaagaag aacaattgct attaatggaa gagtgggaaa
    16741 atctaaatat gagactagat ttgtgagaaa atttcaaaaa caatagtttg tgaaaaaaag
    16801 tacattttaa attgagatgc cttttacgaa aacaaagaaa gcggttcaaa ttcttgcatc
    16861 ttgtgaaaaa aacttataca ataattaagt aataaagatt tttttggagg ttagaatggt
    16921 aactctatct aaagaacaac acttggatat gtttctaaaa atgcaacgta ttcgtgatgt
    16981 tgatatgaaa tttaacaaat tggttcgtcg tggtttcgtc caagggatga ctcacttctc
    17041 tgttggtgaa gaagctgctt cagtaggtgc tatccaagat ttaacagatt cagatatcat
    17101 tttctcaaac caccgtggac acggacaaac tattgcaaaa ggtattgata ttggtggaat
    17161 gtttgctgaa cttgctggta aagcaacagg aacatcaaaa ggtcgtggag gctcaatgca
    17221 cttagcaaac cttgaaaaag gtaactatgg aacaaacggt atcgtcggtg gaggatatgc
    17281 gcttgcagta ggtgctgccc ttacacagca atatgaagga actgataaca ttgttatcgc
    17341 cttttcgggt gactcagcga ctaacgaggg ctcattccat gagtcagtta acttagctgc
    17401 tgtttggaac ttaccagtta tcttctttat catcaacaac cgttatggta tctcaacaga
    17461 catcacatac tcaactaaaa ttcctcacct ttacatgcgt gctgatgcgt acggtattcc
    17521 agggcattat gttgaagatg gtaatgacct tatggctgtt tacgagaaaa tgcatgaagt
    17581 tatcaattac gtgcgttcag gaaatggacc agctattgta gaggttgaat cataccgttg
    17641 gtttggtcat tcaactgctg atgctggggt ataccgtact aaagaagaag ttgattcatg
    17701 gaaagctaaa gatccagtaa aacgctaccg tgcatactta attgaaaatg aaatcgctac
    17761 tgaagaagag cttgcagcta ttgaagcaca agtgattaaa gaagttgaag aaggggttaa
    17821 attcgctgaa gaaagtccat tccctgatat gtctgttgca tttgaagacg tttttgtgga
    17881 ctaaaataac ctaaagtaga tgagatttat ctcgttctat tattcaattg attaaaaaac
    17941 ggatattcgg aggaaataat gagcgaaact aaagttatgg ctttgcgcga agcgattaat
    18001 gtcgctatga gcgaagaaat gcgcaaagat gaaaaagtat ttttaatggg tgaagatgta
    18061 ggtgtatatg gaggagactt cggtacatct gttggtatgt tagaagagtt tggtgctaag
    18121 cgcgttcgcg acacaccaat ctcagaggct gcgattgcag gttcagctat cggtgctgca
    18181 caaacaggtt tgcgtcctat cgttgatttg acattcatgg attttgtaac aatcgcaatg
    18241 gatgctatcg ttaaccaagg tgctaaaacg aattatatgt ttggtggagg tctatcaact
    18301 ccagttactt tccgtgtggc atcagggtca ggtattggtt cggctgcaca acactcacaa
    18361 tcattagaag cttggttaac acacattcca ggtctaaaag ttgttgcacc tggtactgtt
    18421 aatgagtcaa aagcactttt gaaatcttct attcttgaca ataacccagt tatcttctta
    18481 gagccaaaag ctctctatgg taagaaagaa gaagttaata tggaccctga tttctatatt
    18541 ccacttggta aaggggatat taagcgtgag gggactgact taacaattgt ttcttatggc
    18601 cgtatgcttg agcgtgttat gcaagctgct gaagaagttg ctgaggaagg tattaacgtt
    18661 gaggttgttg atccacgtac acttattccg cttgataaag aattaattat cgattctgtt
    18721 aagaaaactg gtaaattgat cttggttaac gatgcttata aaactggtgg tttcactggt
    18781 gaaattgcaa ctatggttgc tgaaagtgaa gcatttgatt accttgatca tcctatcgtg
    18841 cgtcttgcaa gtgaagatgt tccagtaccg tattcacgtg tacttgaaca aggaatctta
    18901 cctgatgtag caaaaattaa agatgctatc tataaagtag ttaacaaagg taaataattc
    18961 ataaaacaaa gagggactga ggaatgagtg aaaacttagc ctctttcctt gtttataggt
    19021 tttaatttat atcgttagat gaaacatata tttgacgata tagtttagtg tgaaaggaaa
    19081 actatggctg tcgaaattat tatgcctaaa ctcggggttg atatgcaaga aggcgaaatc
    19141 cttgagtgga aaaaacaggt aggtgatgtt gttaatgaag gtgacgtcct acttgaaatt
    19201 atgtcagata aaacaaatat ggagattgaa gcagaagact caggtgtttt actaaaaatc
    19261 acacatggga atggagatgt tgttcctgta actgagacaa ttggttatat tggtgctgaa
    19321 ggtgaagaag taacagaagc atcttcttct gaaaatacat cagttgaaga aaacgcgaca
    19381 caagtaactt cagaaccaga aaaagttgaa gaaacatcag aaccaagtgt gccagcagct
    19441 acaagtggtg aaaaagtacg tgccactcct gcagctcgta aattagcaag agagatgtct
    19501 attgaccttg cactagtatc tggaacaggg gcaaatggtc gcgttcacag agaagatgtt
    19561 gaaaacttca agggtgctca accacgtatt acaccattgg cacgtcgaat tgctgaagat
    19621 caaggagttg atatagcaga aatcactggc agtggtattc gtggtaaaat tgttaaaaat
    19681 gatgttttgg ctgctatgtc accgcaagca gcagaggctc ctgtagaaac taaagctaca
    19741 ccaacaacag aagaaaaaca attaccagaa ggtgttgaag tcattaagat gtctgcaatg
    19801 cgtaaggcta tttctaaagg tatgactaac tcttacctca ctgctccaag ctttacactt
    19861 aactatgaca ttgatatgac tgaaatgatg gctcttcgca agaaattaat tgatccaatc
    19921 atggctaaaa caggcttgaa agtcagcttt actgatttga ttggaatggc cgttgttaag
    19981 acattgatga aaccagagca tcgataccta aatgcttcat taattaacga tgcacaagaa
    20041 attgagcttc ataattttgt taatattgga atcgcagttg gtcttgatga tggtttgatt
    20101 gttcctgttg ttcacaatgc tgatcaaatg tcactttctg actttgttat agcatctaaa
    20161 gatgttatta agaaaactca agaaggtaaa ttaaaatcag ctgaaatgtc aggttcaaca
    20221 ttctcaatta ctaacttagg tatgtttggt actaagacgt ttaacccaat tatcaatcaa
    20281 ccgaactcag caatccttgg agtaggagct actattccga ctccaacagt ggttgatggt
    20341 gaaattgtag ctcgaccaat tatggcaatg tgtttaacaa ttgaccaccg tattgttgat
    20401 ggaatgaatg gtgctaagtt tatggttgat cttaagaact taatggaaaa cccatttggt
    20461 ttacttattt gattccgtag gtcttaattt gatataataa gttattcgaa aaacgaaaga
    20521 ggaaataaaa aatggctttt gatgtaatta tgccaaaact tggggttgac atgcaagaag
    20581 gcgaaatcct tgagtggaaa aaaaatgaag gtgacaccgt taatgaaggt gatgtccttc
    20641 ttgaaatcat gtctgataaa accaatatgg aaatcgaagc agaagacact ggtgtacttt
    20701 taaaaattgt acatcaggca ggagatgttg ttcccgtcac tgaagtgatt gcctatatcg
    20761 gagaagaagg tgaagaagta ggtacgtcat caccttcagc tgatgcaact atcacagctg
    20821 aagatggtca atcagtttct ggacctgctg ctccttcaca agagacagtt gcagcagcaa
    20881 ctcctaaaga agaacttgca gctgatgagt acgatatcgt agtcgtaggt ggtgggcctg
    20941 caggttatta cgcagcaatc cgcggtgctc aacttggagg aaaaattgcc attgttgaga
    21001 aaactgaatt tggcggtacg tgtttgaatg ttggttgtat ccctacaaaa acatatctta
    21061 aaaatgctga aatcctagat ggtttgaaag tagcagctgg acgtggtatc aaccttgctt
    21121 caactaacta tgcaattgat atggataaga ctgttgcctt taaaaattct gtagttaaga
    21181 cactgactgg tggtgtacgt ggtctcttga aagcgaacaa agttgaaatt ttcaatggac
    21241 ttggacaagt aaaccctgat aagtctgttg ttattggcga taaagttatc aaaggtcgta
    21301 acgtcgtcct tgcgactggt tctaaggtat ctcgtattaa tattccaggt attgaatcac
    21361 cattagtatt aacatcagat gatattcttg atttacgtga aattcctaag tcacttgctg
    21421 ttatgggtgg tggtgtcgta ggtattgagc ttggtcttgt atgggcttca tatggtgttg
    21481 acgtaactgt tattgaaatg gctgatcgca tcattccagc aatggataaa gaagtatcgc
    21541 tagaacttca aaaaatcctt gctaagaagg gaatgaagat taaaacatca gttggtgttt
    21601 ctgaaattgt tgaagccaat aatcaattaa cgcttaaatt aaacaacggt gaagaagttg
    21661 ttgctgataa agctcttctt tcaattggac gtgttccaca aatgaatggt cttgaaaatc
    21721 ttgagcctga acttgagatg gaacgtggac gtattaaagt taatgcttat caagaaactt
    21781 caattccagg tatctatgca ccaggtgatg ttaatggaac tcgtatgtta gctcatgctg
    21841 cataccgtat gggtgaagta gctgctgaaa acgcactcgg cggtaataag cgtaaagcac
    21901 atctagattt cactccagca gctgtttata ctcatcctga agtagcaatg gtggggatga
    21961 cagaagagca agcacgtgaa caatatggtg acatattagt tggtaaaaat agctttacag
    22021 gcaatggtcg tgctatcgct tcaaacgaag cacatggttt tgtaaaagta attgctgaac
    22081 ctaagtataa agagattctt ggtgttcaca ttattggtcc agctgcagcg gaattgatta
    22141 atgaagcttc tacgattatg gaaaatgagt tgactgtcta cgatgtagca caatctatcc
    22201 atggtcaccc aacattctct gaagttatgt acgaagcttt cttagatgtt ctcggtgaag
    22261 ctattcacaa ccctccaaaa cgcaaataat tttgtttaat atatttgaga ttgagctttt
    22321 atagcttcaa tctcttttca cgtatatatt gtctgaatat tataaaatat tagttaggaa
    22381 tttaaaatga aatatatcgt taacacgtct aatgatccgg cttacaatgt tgctttagaa
    22441 gcttacgcct ttcaaaagtt gacagatatc gatgaaatct ttattctttg gattaatgaa
    22501 ccggctatca ttattggacg ccatcaaaat acaattcaag aaattaacaa agagttcatt
    22561 gataaaaatg gcatccatgt tgtccgtcgt ttgtcaggag gcggagcagt ttatcatgac
    22621 ttgaataatt taaactacac tattatttct aataatacac aagaaggtgc ctttgatttc
    22681 caaacttttt caaaaccagt tattgatact cttgctaaat taggtgttaa agcagaattt
    22741 actggtcgta atgatttaga aatcaatgga caaaaatttg caggaaatgc tcaagcttat
    22801 tacaaaggtc gtatgatgca tcatggttgt cttttatttg atgttgatat gtcagtctta
    22861 gggcaagcac tcaaagtatc taaagacaaa attgaatcta aagggataaa atctgtacgc
    22921 gcacgtgtta caaatattgt tgaccatctt tcagataaaa ttacagtaca agaatttagc
    22981 gatgccattt tagctcaaat gaaggaagaa tatccagaga tggatgaata tgttttgagt
    23041 gatgccgagt tatcagaaat ccaagctatg cgcgataatc aatttgctac atgggattgg
    23101 acgtatggta aagcacctga atacaccatt gaacgtggtg tccgctatcc agctggaaaa
    23161 attacgactt atgctaatgt tgaaaattca acgataaaat ctgtcaaaat ctttggtgac
    23221 ttctttggag tgaaacctgt ggatgatatt gaaaaaatgt tggagggtgt ccgctacgat
    23281 tacaaagatg ttttagcagc gcttaaaaca gttgatacaa gtcaatactt ctcacgtatg
    23341 acacccgaag agattacaaa agctattgta gactaaaatc aaaaaatcta gtcaaagtct
    23401 tcgcctttgg ctaggttttt tgtatggtta agtgacgtta tagtagtaaa aaaacaacgt
    23461 tattgataac gctggttttt atattattca aaatctgctt ttgatttaac gtctccgtat
    23521 tcttcaggaa tctctaaaga taaaatttct tcataactag ggaggacaat ctctttaccg
    23581 tatttgttac ggagggcagt tgccacaaga cggtaagcta gattagcatt acgagaaagg
    23641 attggaccgt gaaagtatga cccaaaaaca ttcttataat gaaccccttc agtaccatct
    23701 tctttgttat tcccatttcc ataaatgacg gttcctaaag gtttttcgtc ttctgataaa
    23761 aaggtacgtc cttgatgatt ctcaaagcca taatatgttt cattgaattc atcgttgtga
    23821 atcttgatat caccaatata gcgattatta ttttggttta gcgtatagtg tcccataacg
    23881 ccaatgccct ctattcgttc tccattagct tgaatatagt attgtcctaa aagttggaaa
    23941 cctccacaaa tagcaagtac tacaccatta ttttgaatga atttgttaat gtcttctttc
    24001 ttactgggaa gatcgcgtgc aacaatggct tgttcgtagt cttgtccacc accaaaaaat
    24061 gccatctgat aatagttggg atcaaatcta tcttctaatg aaacgatatc aaatgtcatt
    24121 tgacatccta gtttttctcc cacatatttc atcattaaga tatttccgtt atcgccataa
    24181 gtatttaata ggttaccata aagatgagct acattaagag tgtatttata atctttggta
    24241 gtaggagatt ttagtgaggt ataagtcatt aattcatctc ctttctgata gcgttgtggt
    24301 tagcaagaat ttcacgaaac tccagcatag ctgtatatgt tgctaaaata taagcatgct
    24361 cagtctcttg ctgctcaatc attgtcataa tatcttgcag tttatcagct tgttttatgc
    24421 gtttctcatc ataaccagtt acacgtaagc gacgagcaat ttcagagtgg cgaacaccac
    24481 cagcaaatat ttctgggata ttcatcgtga gaatagtttc aaaattagcg tcccaaatcc
    24541 aagaagtatc aataccgtca gcataattgg cattcagtag tactgagaga gagaacgggt
    24601 atggagcaag tttaatcata tcaagagctt gactagctcc taccggattt ttaatcagaa
    24661 ctaaagtaca ttttttattt ccaattgtaa aggtttcttg acgaccaaaa acggcacggc
    24721 ttttatcaaa tccatcctta atttgacttg gttctacacc aaaatattca gcaacagata
    24781 ctgccgccaa agcattgtaa atattataaa ggccacccac gttgatatta tattgttggc
    24841 catcaataac gaaacctgat gaagtatttg tcagatgtgt tagccttgtt aaggcataat
    24901 caaggtttgg acgttcaaaa tcacaattag gacaagtgta gtcaccaagg ttggcatatg
    24961 tgttcaatcg ataagttaaa attgcttgac actttggaca gagaataccc tcagtattat
    25021 agtgtgctaa tcgtggctca tgtttgtcgg tattaaatcc atagaattgc acagggttag
    25081 tcacgctttt agaattaaaa agtggactgt caccgttagc taagatagta gcttgcggag
    25141 cgttagcagc tccatctaaa atcatttgat aagttgtgta gatttcgcca taacggtcca
    25201 tttgatcacg gaaaatattg gtaaagacga acaaactagg ctttatatac tgtgtaatac
    25261 ggggtaaact cgcttcgtct atttcaagga cagctatttt tttaccagat ttacttttct
    25321 tagctgttag gaaggtcgaa acaatacctg taatcatatt tgctccactt gggttggtta
    25381 ccacctgacc gaaggcttcc tttaaaatac caactgttaa agctgttgtt aaggttttac
    25441 cgttagtacc agtcacaact acaatttcat aatctttggc aattgtatct agtatatctt
    25501 tatcgaattt gagtgctaaa ctacccggat aagtagagcc acgtcccatt ttacttaaaa
    25561 ggtaatgagc agacttaccg gcagccactc ctagtgctgt atttattttc ataaagatat
    25621 tttatcataa aaagatgctt tcttgctaaa gaaaatatta tgaaaacatg gtataatgta
    25681 gacgatggaa aataagaaat caaggagtta agcaagaaac tcttcgacag gctaccagta
    25741 gagaggaaga tatggatatt ttttcagcta ttgattcaaa gttttgggcg agcattatgg
    25801 aaaatccatg gatgatcctt attcatctca tggatattat catagtagct gttctgattt
    25861 ataaatttat caaagcgcta gcaggcacaa aaattatgtc cttaattcag ggcgttattt
    25921 tgtttatcat cattagattt gtgtcagaat ggatagggct aacaacgatt acatttttga
    25981 tgaatcagat tgttacttat ggtgtaattg caggtgttgt catctttgct ccagagatta
    26041 gaacaggtct cgaaaaattt gggcgaacac cacaactatt tacgcaacgt tctcaattaa
    26101 gcagtgatga aaagctagta gatgcccttg ttaaggcagt tgcttatatg agtccgcgta
    26161 aaataggagc cttgatttca attgaacgta cccaaacttt gcaagagtat atagctacag
    26221 gtatcccatt agatgcagat atttccagtg aattgttaat taatattttc attcctaata
    26281 caccactgca tgatggtgct gttattgtaa aagataaaaa aattgctaca gcatgctcgt
    26341 acttacctct atcagaatca tcttcgattt caaaagaatt tggaacacgc cacagagcag
    26401 cgattggtct atcagagaat tcagatgcac taacagttat tgtttctgaa gagactggtg
    26461 gtatttctgt tgctttgaaa ggtgaatttt tacatgatct ttctaaagat agctttgaag
    26521 caattctgag aactcagttg attcaaaatc aagaggaaaa ctcaaaatta gcttggtata
    26581 atcaactatt gaggaggaag tagacatgaa aaaatttttt acaaataaat tttggttagg
    26641 cgttgtctca ctctttctgg ctattttatt atttttaacg gcaactgcaa caagtatgaa
    26701 ccatcaagac aatagtaaga ttgcaggagc gtctgagaca tatacacata ccctaacaga
    26761 tgtgccaata gacatcaaat acgatagtga tgattacttt attagtggat attcttatgg
    26821 ggcagatgtc tatatgtcat cggttaatcg tgttaagtta gattctgaaa taaatgagga
    26881 tacacgaaaa tttaaagtag tcgccgattt gacaaatatg aaaccaggga ctcacaaagt
    26941 cccactaaag gtagtaaatt tgccatctgg tgtaaatgct acggtttctc caacaacaat
    27001 tacagtaact atgggtaaaa agaaaacaaa agagtttccg gtttatggac atgttaatga
    27061 taagcaaatt aaagctggtt atgcagtcga taagatgtca gttgatgtgt caaaagttaa
    27121 agttactagt gatgaatcta ttatagatcg tattgaccat gtcgctgcca atatccctga
    27181 tgataaagtt cttgatgatg attttaacaa gacagttact ttacaagctg taacagcaga
    27241 tggtacagta ttagcaagta ttattcaccc ttctaaagca actttatctg tcaaagtaaa
    27301 aaaattgacc aagacagtac cgattaattt gataccagta ggacaattca gcgatagtat
    27361 ttcaaaaatt aactacaagt tgagtcaaga aaaagcagta atttcaggaa caaaagaggc
    27421 tttagaagct atttctgtta ttaatgctga ggtagatatt tcagacgtaa ctaaaaatac
    27481 agaaaagaaa attaatcttt ctgcaaacaa tgtttcagtt gatcctgctc aagtaacagt
    27541 tcaattgaca actactaaaa aataatagat aataaaaacg aaaaaaatgg aggtagcgtt
    27601 tttcgctaga aaaaaattat gggaaaatat tttggtacag atggtgttcg cggtgaagct
    27661 aatgttgaac tgacgccaga attggctttt aaattaggac gttttggagg atatgttcta
    27721 agtcaacacg aaactgatcg tccgagagtt tttgtagctc gtgatactcg tatttctggt
    27781 gaaatgctgg aatctgcttt gattgctggt ctactttcag taggtattga ggtttataaa
    27841 cttggtgttt tagccactcc tggagtgtct taccttgtcc gtacagaaaa agctagtgca
    27901 ggggttatga tttcagcaag ccacaatcca gcacttgata atggtattaa gttctttggt
    27961 agtgatggtt ttaaacttga tgatgatcgt gaattagaaa ttgaagcttt acttgatgcg
    28021 aaggaagaca ctttaccacg tccatcagca caaggtttag gtactttagt agattatcct
    28081 gaaggacttc ggaaatatga gaagtttatg gaatcaactg gtattgattt agaaggtatg
    28141 aaggttgcgt tggatacagc aaatggagca gcaacagcct cagctcggaa tatcttttta
    28201 gacttgaatg ctgatataag tgttattggt gatcaaccag atggtttgaa tatcaatgat
    28261 ggtgttggtt cgacacatcc tgagcaatta caaagtttag ttagagaaaa tggttccgac
    28321 atcggtctag cttttgatgg tgatagtgat cgtttgatcg ctgttgatga aaatggagag
    28381 attgtagatg gtgataaaat tatgtttatc attgggaaat atctctctga taaagggcag
    28441 cttgctcaga atacaatcgt aacaacggtt atgtcaaatc tcggctttca taaagcgctc
    28501 gaccgtgaag gaattcataa agcgattacc gctgtaggag accgctacgt ggttgaagaa
    28561 atgcgtaaat caggatataa tcttggcgga gagcaatcag gccacgttat tatcatggat
    28621 tacaatacta caggggatgg acaattaaca gccatccaat tgactaaggt catgaaagaa
    28681 actggtaaga aattatcaga gttagctagt gaagtgacga tttatcctca aaaattagta
    28741 aatattcgtg ttgaaaataa catgaaagat aaagcgatgg aagttcctgc aattgcggaa
    28801 attattgcta aaatggaaga agagatggat ggcaatggtc gtattctagt acgtcctagt
    28861 ggtactgagc ctcttcttag agttatggca gaagcgccaa caaatgaagc ggttgattat
    28921 tatgttgata ctatcgcaga tgtcgttcgt acagaaattg gtttagatta aaacggcgtt
    28981 attctgctat aaagtcgtca atgttataaa ataataaaga gatggaattg gagtggagca
    29041 tcaagcatac gcctttctct ctctttttga tttgagaagg gagtttagag aaaatgacta
    29101 aagaagcaga attattatgg gctaaacaca agtatttagt cttaagtaaa tctcagaaaa
    29161 tttatttgga tattagacaa actttgaaat caccaaattg taccgtttta gacgttcagt
    29221 cactcattga ccaagcagtg cttttagaag aatcaccatc acaagtgact aatgcttata
    29281 tgcatatatg gggctatttt aaaaataagg cagagcgtca agaaaaagaa gaatttttaa
    29341 ccttgctgga aaaatacagg aaaacaggtt atcaacgaag aaaactgtta gcttttctaa
    29401 aacagctatt agctaaatac ccaaattctt atttacaaaa ctcttctatt ttcgaagaag
    29461 agtaaaagat tgatagttga ggtatataaa tgagattatg gcatcaagat attattgagt
    29521 tattgcctag gcagcaactt ttaggacagc atagagagtg ttgtgcattg agagggaatg
    29581 ggtgggggag aaaacatgag actgttgatt atgtttttcg atattcacct tataggttat
    29641 ttgcatatca tcaattagtt atggaagaaa tgatggagcg tgggtatcgt gtatctaaag
    29701 aatggctaat tgcagaatat aggggaatga agtgcccaag atatgataca cttaatcctg
    29761 ttgacttgga gacacctatc tatcccgagc acaatcaaga ctatctacaa gaatgtctat
    29821 ggaatttaaa agcaaaagga attgaccttc ctataaataa gataaaatag agatttatct
    29881 ctattttatt ttggaataaa aagctatatt atcaagtttt agaggagtgt ttttttaagg
    29941 gcaaagtggt aaaatagaaa catgttaaag aaaccaacct cagcatatgt acacattcca
    30001 ttttgtaccc aaatatgtta ttattgtgat ttttcaaaag tttttattaa aaatcagcct
    30061 gtagacgctt atcttcaggc tcttatccgt gaatttcggt cttatgatat tacagaattg
    30121 cgcaccttat atattggagg agggacacct acatctataa gcgctgtgca actggactat
    30181 ttattgacgg agttaagtag agatttaaac ttaaatactt tagaggagtt cactatcgaa
    30241 gctaatccag gagatttaac cgttgataaa atagaggtgc ttcaaaagtc agctgtcaat
    30301 agagtttctc taggagttca gacctttaat gataaacatt taaagcgtat tggacgcagt
    30361 cacaatgagg cacaaattta ttctacaatt gatgctctta aaacagctgg ttttcaaaat
    30421 atctctattg acctaattta tgccttacct ggacaaacga tggatgatgt tagaagtaat
    30481 gtagccaaag ctctctctct aaatattcct catttgagtt tatatagttt aattttagaa
    30541 caccatactg tatttatgaa taaaatgcgt cgtgggaaat tacatttgcc aacagaagat
    30601 ttagaagccg agatgtttga atatatcatt tctgaaatgg aaagaaatgg ttttgagcat
    30661 tatgaaattt ctaattttac aaagccgggt tttgaaagtc gtcataattt gatgtactgg
    30721 gataatgtag aatattatgg tgttggtgca ggcgcttctg gttatcttga tggtattcgt
    30781 tatcgtaata gagggccaat acaacattat ctaaaggggg tttcagaagg taatgcaagg
    30841 ttatctgaag aagtattatc aaaaaatgaa atgatggaag aggagctttt cctcggtctt
    30901 cgtaaaaaag agggtgtttc catcggaaaa tttgaacaaa aatttggaac gtcctttgag
    30961 aaacgttacg gtcagattgt tcaagaactc caaagtgatg ggcttttgaa agaaaataat
    31021 ggttttatcc agatgactaa aaaaggtctc ttcttaggag atacggttgc cgagaaattt
    31081 attgtggagt aaaaaatggg tttattatat cgagaaactt acgaagtgcc tttttatgaa
    31141 tcagacacta atcattatat gaaattaccg cagttgttgg ctttagcgtt acaaatttca
    31201 gccaaacaat ctttaaaatt aggaataggc gatgacatcg tttttaaaag atacggattg
    31261 gtttgggtcg ttacagatta tattattgat attgagcgtt tacccaagca tgctgaaaag
    31321 atagtcattg agactgaagc caaagctcat aataaattat tgtgttaccg ctatttttat
    31381 atttatggtg aagacgggca aaaaataata acgatttcat ctgcttttgt cttaatggat
    31441 tttaaaacac ggaagattca tcctgttctt gatgatatta cttcaattta ccaatcccaa
    31501 agaatcaaaa aagttattcg agggccgaaa tatcatccca ttggagatag taaagttaaa
    31561 caataccatg ttcgttattt tgatttagat atgaatggac atgttaataa tagtaagtat
    31621 ctagaatgga tgtatgatgt gttggactta gattttctaa gtagccacat tcctaaaaaa
    31681 attgatttaa aatatattaa ggaaattcaa tatggaacgg atattaagtc tcattggtat
    31741 caagatggtc ttgtgacacg tcatgatatt attggtggcg atgctattca tgctcaagct
    31801 aggattgaat ggcaggaaaa aaaggaggat taaatggctt ataaaggata tcttattgat
    31861 ttagatggta ctatttacaa agggaaaagt cgtatcccag caggagagcg ttttatcgaa
    31921 cgtttgcaag aaaaaggcat tccttatatg ttagtaacta ataatacgac tcgtacacca
    31981 gaaagcgttc aggaaatgct tcgaggcttc aatgttgaaa caccacttga aacaatctat
    32041 accgcaacta tggctacggt tgactatatg aatgatatga accgtggaaa aacagcttac
    32101 gttattggtg aagaagggct taaaaaagct attgcagatg caggttatgt ggaggataca
    32161 aaaaatccag cctatgtggt tgttggttta gattggaatg ttacttatga taaattggca
    32221 actgctacgt tagcaattca aaatggtgct ttatttattg gaaccaatcc agatttgaat
    32281 attcctactg aacgtggttt attacccggt gctggctcct taaatgcttt attagaagcc
    32341 gcaacgcgta taaaaccagt ctttattgga aagccaaatg ccatcattat gaataaggca
    32401 cttgaaattt taaatattcc tagaaatcaa gcagtcatgg tcggtgataa ttatttgaca
    32461 gatatcatgg ctggtattaa taatgatatt gatactttgt tagtaacaac gggttttaca
    32521 acagttgaag aagtaccaga tttaccaatt caaccaagtt atgttttagc aagtttagat
    32581 gaatggacat ttaatgaagg ataagttatt agtagtttta acttggattt ggattatatc
    32641 gttagcaact ctagctacaa tttatattgc ttggttgatc tatcctatag aaattcagtt
    32701 tttaaaatta gaaaaggttg tgtatttaaa ggctgaaaca atttattata attttaataa
    32761 gt
//
LOCUS       DATRSD010000011        30500 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRSD010000011 DATRSD010000000
VERSION     DATRSD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30500
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.011"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            102..887
                     /locus_tag="VBD85_000424"
     CDS             102..887
                     /locus_tag="VBD85_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7132999.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            complement(965..1603)
                     /locus_tag="VBD85_000425"
     CDS             complement(965..1603)
                     /locus_tag="VBD85_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO7133000.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            1820..2476
                     /locus_tag="VBD85_000426"
     CDS             1820..2476
                     /locus_tag="VBD85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133001.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            2473..3246
                     /gene="fetB"
                     /locus_tag="VBD85_000427"
     CDS             2473..3246
                     /gene="fetB"
                     /locus_tag="VBD85_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO7133002.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            3410..4228
                     /locus_tag="VBD85_000428"
     CDS             3410..4228
                     /locus_tag="VBD85_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO7133003.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(4265..5149)
                     /locus_tag="VBD85_000429"
     CDS             complement(4265..5149)
                     /locus_tag="VBD85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7133004.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            5342..6400
                     /locus_tag="VBD85_000430"
     CDS             5342..6400
                     /locus_tag="VBD85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7133005.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            6414..7406
                     /locus_tag="VBD85_000431"
     CDS             6414..7406
                     /locus_tag="VBD85_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO7133006.1"
                     /translation="MKLKVFNVREEEATLAKDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            7613..9163
                     /locus_tag="VBD85_000432"
     CDS             7613..9163
                     /locus_tag="VBD85_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO7133007.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            9230..10249
                     /locus_tag="VBD85_000433"
                     /pseudo
     CDS             9230..10249
                     /locus_tag="VBD85_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            10266..12065
                     /gene="uidA"
                     /locus_tag="VBD85_000434"
     CDS             10266..12065
                     /gene="uidA"
                     /locus_tag="VBD85_000434"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO7133008.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            12094..12765
                     /locus_tag="VBD85_000435"
     CDS             12094..12765
                     /locus_tag="VBD85_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO7133009.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            12882..13499
                     /gene="eda"
                     /locus_tag="VBD85_000436"
     CDS             12882..13499
                     /gene="eda"
                     /locus_tag="VBD85_000436"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7133010.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            13516..14916
                     /gene="uxaC"
                     /locus_tag="VBD85_000437"
     CDS             13516..14916
                     /gene="uxaC"
                     /locus_tag="VBD85_000437"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO7133011.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNVFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            14934..15980
                     /gene="uxuA"
                     /locus_tag="VBD85_000438"
     CDS             14934..15980
                     /gene="uxuA"
                     /locus_tag="VBD85_000438"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO7133012.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            16104..16943
                     /locus_tag="VBD85_000439"
     CDS             16104..16943
                     /locus_tag="VBD85_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7133013.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            17096..17908
                     /locus_tag="VBD85_000440"
     CDS             17096..17908
                     /locus_tag="VBD85_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7133014.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            17924..19714
                     /locus_tag="VBD85_000441"
     CDS             17924..19714
                     /locus_tag="VBD85_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO7133015.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMARRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(19772..20857)
                     /locus_tag="VBD85_000442"
     CDS             complement(19772..20857)
                     /locus_tag="VBD85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7133016.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            21067..22071
                     /gene="ccpA"
                     /locus_tag="VBD85_000443"
     CDS             21067..22071
                     /gene="ccpA"
                     /locus_tag="VBD85_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO7133017.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            22203..23669
                     /locus_tag="VBD85_000444"
     CDS             22203..23669
                     /locus_tag="VBD85_000444"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO7133018.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGKQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVESASISAWVPKIEEAN"
     gene            23714..24712
                     /locus_tag="VBD85_000445"
     CDS             23714..24712
                     /locus_tag="VBD85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7133019.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKESAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYHVAESRSIERIAHELASVYQKVMEL"
     gene            24714..26048
                     /locus_tag="VBD85_000446"
     CDS             24714..26048
                     /locus_tag="VBD85_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7133020.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            26505..28448
                     /gene="thrS"
                     /locus_tag="VBD85_000447"
     CDS             26505..28448
                     /gene="thrS"
                     /locus_tag="VBD85_000447"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO7133021.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIQDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARV
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            28670..29374
                     /locus_tag="VBD85_000448"
     CDS             28670..29374
                     /locus_tag="VBD85_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133022.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGRIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            29472..30491
                     /locus_tag="VBD85_000449"
     CDS             29472..30491
                     /locus_tag="VBD85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO7133023.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
BASE COUNT         9951 a         4621 c         6198 g         9730 t
ORIGIN      
        1 ataggcatat gatatggttg tgacaaaagg agagcaatta gctaaaacgg atatcaatat
       61 ggtagacgct tgtttaatgt aattagaaag aggtaaagct tatgaaaaga ttacataaac
      121 tgtttataac cgtaattgct acattaggta tgttgggggt aatgaccttt ggtcttccaa
      181 cgcagccgca aaacgtaacg ccgatagtac atgctgatgt caattcatct gttgatacga
      241 gccaggaatt tcaaaataat ttaaaaaatg ctattggtaa cctaccattt caatatgtta
      301 atggtattta tgaattaaat aataatcaga caaatttaaa tgctgatgtc aatgttaaag
      361 cgtatgttca aaatacaatt gacaatcaac aaagactatc aactgctaat gcaatgcttg
      421 atagaaccat tcgtcaatat caaaatcgca gagataccac tcttcccgat gcaaattgga
      481 aaccattagg ttggcatcaa gtagctacta atgaccatta tggacatgca gtcgacaagg
      541 ggcatttaat tgcctatgct ttagctggaa atttcaaagg ttgggatgct tccgtgtcaa
      601 atcctcaaaa tgttgtcaca caaacagctc attccaacca atcaaatcaa aaaatcaatc
      661 gtggacaaaa ttattatgaa agcttagttc gtaaggcggt tgaccaaaac aaacgtgttc
      721 gttaccgtgt aactccattg taccgtaatg atactgattt agttccattt gcaatgcacc
      781 tagaagctaa atcacaagat ggcacattag aatttaatgt tgctattcca aacacacaag
      841 catcatacac tatggattat gcaacaggag aaataacact aaattaataa aagagcaact
      901 aattgctctt tttttgttgt cattcttagt gattaatagt gttaaataga aatttttgga
      961 tacattactt agtgttccga tatgatttga caccagaaat aaggacagga attaaggtaa
     1021 caaatacaat tcccaagata ataagcgaga agtgctgctt cacaaaagga atattaccaa
     1081 agagaaaacc agaacctgtt gcaataactg accaagataa tgctgcaatg aaggcgcgtt
     1141 tgataaatac ttttggaggg agctgactaa tacctgctac aaaaggaaca aaggttctga
     1201 taatagggat atagcgccct aaaatgatgg cagcagtacc ttttttctca aaataaagct
     1261 cagcatctcg gatatttttt tctttaatga aacgacgaaa gaaaggatgt ttgataaaac
     1321 ggtaaccaaa tgtacgacca atcaagaagt tgcaactatc accaataaat gctgcaataa
     1381 agaaaataat aagaaaagtt acaatattga aggacatttt tggatttgca gcaagggctc
     1441 cagcagcaaa taataaacta tcacctggta aaaatggaaa gatgacagcc ccagtttcaa
     1501 cgaaaataac gaggaagagc aatagatagg tccaattacc aacagtgtta gccatagcat
     1561 agatatgagt atcaatatgt aaaataaaat cgattaaaaa cataggagtc tcctttattg
     1621 atcaactaaa gtgcagagac ttggatgata actatcttgt ttataaggcc tcttacaaag
     1681 cctattttat cagaaaattt ttaataagag gtaaaataag tttacttatt aatttagaat
     1741 tattctaaag agcttttttt tgatttttat gttagactta attataatga ttataaataa
     1801 gaaataaagg agcttaacaa tgtcctattt aacctttaaa cacgttgatt ttaaaacgga
     1861 tgataagttg gttttaaatg atatcaactt tgcaattgat gaaggtgatt ttgtttctat
     1921 tgtagggcca tctggaagtg gtaagagtac tgtattaaaa ttagccagtg gtttaatgag
     1981 tcctactgca ggtcatattt tctttgatgg aaaagatttg aaccaattag aaccgattga
     2041 aagtcgaaaa atgatttctt actgttttca aacgccacat ttatttggta atacagtgga
     2101 agacaatatt agttttcctt accacattcg acacgaaaaa gtagattata gacgcgttga
     2161 tgatttattt caacgttttg aaatggacca gtcttacctg aaacaggatg tcaaaaaact
     2221 atcaggagga gaaaagcaac gtattgctct gattaggcaa ttattgtttg aacctaaagt
     2281 tttactctta gatgaagtta cttcggcttt agacaatcat aataaagcta ttgttgaaaa
     2341 ggttattaaa tcattacatg acaaaggaat aacgattttg tggattacgc atgatgagga
     2401 acagagtcgt cgatttgcca ataaagtctt aaaagttgtc aatggtagta ttgaagcaat
     2461 ggaggttata aaatgagcgg tgcaaattct atttctttaa tgtcattact aatggcatcg
     2521 tcattagttt tgattacttt atttttttct tactggcaaa agttaaacct agaaaaagaa
     2581 gtgattatta gcgctatccg agcagtgatt caattattag cagtgggttt tttgctagac
     2641 tatatatttg gttatcaaaa tcctattttc acagctcttt taatgctatt catgattatt
     2701 aatgcttctt ataacgcagc taagcgtggt aaagggatta ataaagggtt tgttatttca
     2761 tttatagcta ttgggtcagg gactataatc actttatctg tcttgatttt ttcaggaatt
     2821 ttaaaatttg ttccgaatca aatgattcct gtaggtggta tgattattag taattctatg
     2881 gtagcaatcg gtttgtgcta taaacaatta ttgtccgaat ttagaagtaa acaagaggaa
     2941 gtggagacaa aattagcttt aggagcagat attttgccag cttcgattga tattattcgt
     3001 gatgttatca aaactggtat ggtacctaca attgattcag ctaaaacact tgggattgta
     3061 tctttgccag ggatgatgac agggttaatt cttgctggaa cttcacctat ccaagcggtt
     3121 aaataccaaa tgatggtgac gtttatgcta ttagcaacga cttcgatagc ttcttttgta
     3181 gctacttatt tggcttataa aattttcttt aataatcgta agcaattagt agtaacaaaa
     3241 gattgattca ttttaataaa cagtatgtta gtctggtaag tatagtccag gcttttttgg
     3301 tatcttaatc ttatttttga tcgaaaattg accaagttat tgagtcgatt acgtttaaat
     3361 gctactattt aatagaaatt gttgtaggga ttatcaaaag gaggaagaaa tgattttaaa
     3421 aatttgtcgt gcagcatata gtttacaatg gggaggtgtt taccaattag ctttgctgga
     3481 ttatcctcga attaaggcgt ttgaattgga aaggatagga gctttcatag cttacgagaa
     3541 acaatataaa agaaaaactg agatacaatg tgacgataaa catctcctcg caaaaattgt
     3601 tcatttttta aaatacaata gttttacttt tccctatatt cccaaatata gagaagcggc
     3661 agctactttt aatgaggatg gtattagttt aacttctgat tttttaagcc atacatgtac
     3721 gattgaaact gcaaaactaa tttttaaaga aggtaaaatc ttatcagcag ttaaagcctt
     3781 taataagcct gctgaagtac tggtaaaaga taagaggaat gctgctggag accctaaaga
     3841 ttactttgac tatgtgatgt tgaactggtc aaataccaat tctggttatc gtttagtaat
     3901 ggaaagattg ttaggcaaag caccatctga acaggagtta acagtaggtt ttaagccagg
     3961 ggtcagtttt cattttactt atcaagatat catcaatcat cctgattcta tttttgatgg
     4021 ttatcatcct gctaaaatta aaaatcagct ttctttagca gaacatttag ttgcatgtgt
     4081 tatcccaaaa cattatcaag aagattatca aagccttgtg cccaatgact tgaaacacag
     4141 ggtttattat ttagattact gtaacgaaac actttatgag tggaatcaaa aagtttatga
     4201 ttttctttgt catttggaaa ataaataaaa ccaaaaagtt taaaactttt tggtttaact
     4261 gctattaatt ggtctttaaa agacttaaaa attcttcaat cataggacta aaagaagtat
     4321 catttcggta agcaatacca atcggataaa aattattttt atccttaagt gggacccagc
     4381 ataaatcttg aacttggcta atgctttcaa attcacttgg taaaatagtg actgcattga
     4441 ggtcttgtaa actatgaata agaacttccc agtcattgtg tttaaagacg atatgggggt
     4501 caaatcctaa agcacgacat ttacgaggca acatctctcc tagcatataa tgttcgttga
     4561 gtgaggcaac tttataatct cttaaatcat taagttcaat ttcaggaagt gttgcaagag
     4621 gatgcgattt tggcatgaca atgcttactt tatatccttt agttgaggtt tgcagtggtt
     4681 cgatagtgat atcattgcgg gtacttggaa aggacaataa gccaatatcg atctttcctt
     4741 ttgctaacaa ttcttgtaat ttacgggaac catcttgaat caaagatact tcaacatttg
     4801 aatgtgtagc cataaaagtt gaaatctgtt tcataaattg aatagcaaaa agagaagtaa
     4861 gccctacacg gataccttcc tttttttctt gatttaattg ctggatctcc gtaaccattt
     4921 gattaacatt agtcatcaac aattgtcctt tctcatagag aatttctcct gcttcagtta
     4981 aagcgagttg acgaccattt tgtaagaata aaatagtatt taattctgct tctaattttt
     5041 taatggattg tgaaagtgta ggttgggtca caaaaagact ttttgcagcg cgtgaataat
     5101 tttttgcttc tgcaacagca ataaagtaag taagttgtct aatatccata atatctgtcc
     5161 ttttttctta gtaatagtat agcacaagtc tatcaatctg acctttatga ttatttccac
     5221 aataatatta gtaaaagcta tacttatata attttttcca attttccttt tgcaaatagc
     5281 tatgatatag ttaatatagt tagtgaaatc acaaacaatc attattagta ggaggcatct
     5341 tatgtcggat gttgtcgaaa aacaaacaac taaatcgttt ataatgaacg tattaaatgg
     5401 tttagcttta gggactgtta ttgtcttgat accaggagca atccttggag aattaatgaa
     5461 agctttactg ccaatgtgga gcggctttgc aactttaatc gctgcaacag cagttgcaac
     5521 aagtatgatg gggcttgtta ttggtattat ggttggtctc aattttaaat ttaatcccat
     5581 tcaatcagca tcactaggat tagcggttat gtttgcaggt ggagctgcaa ccttccttaa
     5641 aggcgctatt atgctaaaag gaacaggcga tatcattaac atgggaatta ctgctgcctt
     5701 aggtgtcctt ttgatccaat ttttatctga caagacgaaa tcattcactt tgattgtcat
     5761 tccaacagta acactcttac tagttggagg agtaggtcat gtgttattac cttatgtcaa
     5821 aatgattacc accatgattg gacaagggat tgcttcatta cttggtttac aacctgtttt
     5881 gatgtcaatt ttaattgcga tgattttctg tttcttgatc gtatctccaa tcacaacagt
     5941 tgggattgct ttggcaatta gtttgtccgg aattggttct ggtgctgcta atttaggtat
     6001 ctgtgctgct agttttgggc tttgtatggc tgggtggtca gtcaattcta aggggacagc
     6061 cttagcgcat gttttagggt ctcctaagat ttcgatggct aatgtattag caaaaccgaa
     6121 gattatgcta cctatgatat cttcagcagc aatactagga attctcggag ctctctttaa
     6181 tattcaagga acaccagcta gtgcaggttt tggtatcagt ggtttgattg gaccgattaa
     6241 tgctcttaat ttagcaaaag gtggatggtc agttatgaat atgttattga taattataat
     6301 atttgttgct gcaccaatta tattgaattt tatttttaat tacctcttta taaaagtact
     6361 taaaattatt gatccaatgg actataaatt agatatttaa ggagatatta gtgatgaaac
     6421 tcaaagtatt taatgttaga gaagaagaag caacattagc aaaagattgg gcaaaccgaa
     6481 atcatgtgga actttctatg agtgaaggac ctttgacatt ggaaactgtt aatgaggtag
     6541 aaggttttga tgggattgct aatgcacaga ttgagccttt agatgatgct atttatcctc
     6601 ttctcaaaga aatgggaatt aagcaaatcg ctcagcgcag tgctggtgtt gatatgtata
     6661 atcttgagtt agctaagcaa catggtatta tcattagcaa tgtgccaagt tactcaccgg
     6721 agtctattgc agaatttaca gtgacaatcg ctcttaacct tattcgaaaa gttgagctta
     6781 ttagagctaa tgttcgtgaa cagaattttt catggacctt gccaattcga ggacgagtcc
     6841 taggtaacat gactgtggct ataataggaa ctggtcgcat tggtctagcg actgctaaaa
     6901 tttttaaagg gttcggatgt cgtgttattg gttatgatat ttaccataat ccaatggcag
     6961 atggtatcct tgagtatgtc aattcggtag aagaagctgt tgaagaagct gatcttgtgt
     7021 ccttacatat gccaccaact gctgaaaata cgcatctatt taatttagat atgtttaaac
     7081 agtttaaaaa aggtgctatt ttaatgaata tggcacgtgg agcattggtg gaaaccaaag
     7141 atcttcttga ggctttagat caagggcttt tggagggagc tggtattgat acttatgaat
     7201 ttgaggggcc atatatacct aaaaattgtc aaggtcaaga catttcagac aaggatttcc
     7261 taagactgat taaccatccg aaagtaatat acactcctca tgcggcttat tatacagatg
     7321 aagcggtcaa aaatctagta gagggtgcct taaatgcttg tgtagaagtg attgaaacgg
     7381 gaacaactac tacgaaagtc aactaaaaga tgcttaggca tctttttttg tggggtaaca
     7441 aaacatatga tgatcatgac ctttaaagca aaaaaacatc ttatgtattt atagctggaa
     7501 agtctttttt ataataaaaa aagatatttt ttataaaaat attgaaaaaa catcagatga
     7561 atagtattat ttaatcataa aagcgtttac aaaatacaag gagggttctc tcatggagaa
     7621 aacaatggat atggaacaac gtgaagaagt tgtatcttat aaccgagcga aagtatggca
     7681 gttagttatg tttgccatga acaatgcttc tacaaatata tacttattta cgtttatgtt
     7741 tgttacctat ttctcaactg gcgttcttgg ctcggcagcc atttttgtca gtcaaattat
     7801 gggttatatc cgtatttttg atggttttat tgatccagct attggtatta tgattgataa
     7861 aactgataca aaatttggta agtatcgtcc tattttgatt attgggaacg ttattacggc
     7921 tttgtcattg atttttcttc ttgcattgag aggtgtcgat gaaaatattc gtttcccatt
     7981 atttatcctt gttctaatta tccataaaat tggatactca atgcaacaga caataactaa
     8041 agcagggcaa acagcactga caaatgaccc taagcaacgt cctattttta atatcgttga
     8101 tgcagttatg acaacatcat taatgacagg cggtcagttt gtcgtttcag ttttcttagt
     8161 accaaaattt ggtaatttta cacctcaatt cttcaatgtc cttatttttg gaacaattct
     8221 tatttcagct attttagcaa tcgttgcaat tattgggatt tgggcaaaag acagaaaaga
     8281 attctttggt ttaggagaga atacccaaaa gactgctcta aaagattact ggaaggtgtt
     8341 gaaaggaaat aaaccgcttc agatattatc cattgctgca gctttggtaa aatttgccat
     8401 ccaattcttt ggcgattccg ttgttatggt gctattgttt ggtattttat ttggtaatta
     8461 tgctttatca ggacaattct cacttctttt cattgttcct ggtgttatta ttaatatttt
     8521 gttctcaaca attgctcgta aaaaaggtct tcgtttttca tatgtcagag caattcagat
     8581 cggtatgatt ggtttacttg cttttggtgc agttctttac gttggtaagc ctggtgacct
     8641 tagtttaaca agcctaaatc tttatactat tttatttatc gttactaata ttattgcgag
     8701 atacgcttca caagcacctg ctagtttagt attgactatg ggagcagata tttctgacta
     8761 tgaaacttct gaatccggtc gttatgtatc aggaatgatt ggaactatct tctccttaac
     8821 agattcgatt gcttcttcat ttgctcctat ggtagtaggt tttgtgttag caggaattgg
     8881 attttcaaaa tcatttccaa ctatcgaaac accactgccc cctgatttaa aaatggcagc
     8941 tattagtatc cttgtggcca ttccatttat agctttgtct atagctcttc ttttaatgaa
     9001 attttacaaa ctagataaag aagaaatggt tcgtattcaa gagaagattc aagttatgaa
     9061 agcagcaact actaaggaac gtgctaaaga tattgctcgt aatgttcctt tgacagatat
     9121 ggattatgtt gatgtaacaa aatatcctat tgataaagat taaaaagttt agctgctctt
     9181 caatagagca gctattttgt taaataagag ctaccattgg aggattctta tggcaaaaat
     9241 tatttcatta ggtgaggttc ttttgcgatt atctccacct caataactga tgcaggctaa
     9301 tcacctaaaa tgccaattcg gtggttcaga acttaatgtc ttagcaagcc ttgctcaact
     9361 aggttaccat gtaggattgg tctcagcttt gcctgataat gatttgggca agatggcaag
     9421 tcaatttata ttgtcacagc aaattagtcc agctgctata attaaaaaag aaggacgact
     9481 aggaatttat tactatgaac aaggattttc ggtaagaaca aataaggtta tctacgatcg
     9541 taactattca tctttttggg aaagtacctt gagtgattat gattttactt ctatttttaa
     9601 gggagtggat tggtttcatg tttctggaat tactccagca ctaaccaagg acttatatga
     9661 ggtgactcgg ttccttatga caaaagctaa agaaggaggt gtaaaagtat cgattgatct
     9721 caattttcgt gagagtttat ggtcatcttt tcaagaggca cgtgagcaat tatcgccact
     9781 attaggatta ttagatgttt gctttggctt agagccgatt tatttagctg gcgaaagtga
     9841 agatttaaaa gatgagttgg gcttatccag accttacctt gatattgaat tacttgaaaa
     9901 gataacccaa aagattgtcc aagaatatgg gttagactat attgccttta ctcaaaggga
     9961 aatggaatat actaatcagt atatgttaaa atcatatttg tatcataata atatgttata
    10021 tcaaactgat aaaactggcg ttgaagtatt agatcgtgtg gggacaggag atgcctttgc
    10081 tgcaggttta attcatgcgc ttctggaaaa agaaactcct caaagggcct tagaaatagc
    10141 tatggctaca tttaaataca aacataccat tcaaggagat atcaatatca tgacacgaga
    10201 tgatattgct tacctcattg aaaaagaaac aaatgatatt aaacgatagt gtaaaaggag
    10261 agtgtatgtt atatccatta ttgacaaaaa caagaaatac ttatgattta ggcggtattt
    10321 ggaactttaa attaggagaa cataatccaa atgaattact accttcagat gaagtcatgg
    10381 ttatcccgac ttcatttaat gatttgatgg taagtaaaga aaaacgtgat tatataggtg
    10441 atttttggta tgaaaaagtc attgaagtac ctaaagtatc agaggatgaa gagatggtgc
    10501 tgcgttttgg ctcagtgaca catcaagcta aaatttatgt tgacggtgta ttggtaggag
    10561 agcataaggg aggttttact ccttttgaag ttcttgttcc tgaatgtaag tataataatg
    10621 agaaaattaa ggtatcaatt tgtgctaata acgttttaga ctataccacg cttccggtcg
    10681 gaaattatag tgaaatcatt caagaagatg gtagcattaa gaagaaagta cgtgagaatt
    10741 ttgatttctt caattatgca ggtgtgcatc gtcctcttaa gttgatgata cgtcctaaaa
    10801 atcatatttt tgatattaca attacaagtc gtctatcaga tgatttacag tcagcagatt
    10861 tacatttttt agtggagact aatcaaaagg ttgacgaagt tagaattagc gtttttgatg
    10921 aagataacaa actagtgggt gaaacgaagg atagcagatt atttcttagt gatgtccatc
    10981 tttgggaagt tttaaatgcc tatctgtaca cagcgcgtgt tgaaattttt gttgataatc
    11041 aactccaaga tgtctatgag gaaaattttg gtcttagaga aatagaagtg acaaatggtc
    11101 aattcctatt gaatcgcaaa cctatttatt ttaaaggatt tggtaaacat gaagatacgt
    11161 tcattaatgg cagaggtttg aatgaagctg ctaacttaat ggatttgaat cttttgaagg
    11221 atatgggggc aaactctttt agaacatccc attatcctta ttctgaagaa atgatgcgtt
    11281 tagcagatcg aatgggagtg ttagtgatag atgaggttcc agcagtaggg ttatttcaaa
    11341 attttaacgc ttccttagat ttgtcaccaa aagataatgg tacgtggaat ttgatgcaaa
    11401 caaaagcggc gcatgaacaa gctatccaag aattagtgaa gcgtgataaa aatcatccta
    11461 gcgtcgtgat gtgggtagtc gctaacgaac cggctagtca tgaagcggga gcacatgatt
    11521 attttgagcc attagtaaaa ctttataaag atttagaccc tcaaaaacgt cctgtcacct
    11581 tggttaatat tttaatggca accccagata gagaccaagt gatggacctg gttgatgttg
    11641 tctgccttaa tcgttactat ggttggtacg ttgaccacgg tgatttaaca aatgcagaag
    11701 taggtataag aaaagagtta ttagaatggc aagataaatt tcctgacaaa ccaattatca
    11761 taacggagta tggcgctgat acgttaccgg gattacattc tacttggaat attccatata
    11821 cagaagaatt tcaatgtgat ttttatgaaa tgagtcatcg tgtttttgat ggtattccta
    11881 atttagttgg tgagcaagtc tggaattttg cagactttga aactaatctg atgatacttc
    11941 gtgtacaggg gaatcacaaa ggtctctttt caaggaatcg ccagccgaaa caagtcgtca
    12001 aagaatttaa aaaacgctgg atgactattc ctcattacca taataaaaaa aatagtgtaa
    12061 aataggggta gttattttag aagaggtgat gatatggcta gacccttagt agaacaaaca
    12121 gcagatcgtc tattgcattt aattttagaa agagaatacc ctgttggtgc taagctcccc
    12181 aatgaatatg aattagcaga agatctagat gttggtcgca gcacaatccg agaagcagtc
    12241 agaagtttag caacgcgtaa tattttagaa gtcagacagg gctctggaac atatattagt
    12301 tctaaaaagg gtgtttctga ggatccatta gggttctctc taataaagga cacagatagg
    12361 ttgacatcag atctctttga actacgcctt ttattggaac ctcgaatagc agagctggta
    12421 gcttatagaa ttacagatga tcagttacaa ttactcgaaa agcttgttgg agatattgaa
    12481 gatgctgttc acgcaggcga tccgaaacat ttacttttag acgtggaatt tcattccatg
    12541 ctagctaaat acagtggtaa tattgcaatg gatagccttt tgccagtcat caaccagtca
    12601 attcatctga taaatgcgaa ttatacaaat cgtcagatga agtcggatag tttagaagca
    12661 catagagaaa ttatcaaagc aatccgagaa aaaaatccag tagctgcaca tgatgctatg
    12721 cttatgcata tcatgagtgt tagacgttca gcattgaaag cttaatataa gaagaaaaat
    12781 agctattctt gaatagctat ttttttagta ttcttcttga caattcatca gatgacttat
    12841 ataatatagt taaagataac ggttacattt tgggaggtat tatgttaaat caattgaaaa
    12901 acaactattt ctttgctgtt attcgaggga aatcatctga ggatgcgctc gaaatcgcaa
    12961 agcatgctat tttgggtggt attcggaata ttgaagtaac tttttcgact cctgaagcaa
    13021 gcaaagtcat caaacaatta tcagatgact ttaaaaataa taaagaaatt attgtggggg
    13081 ccggaacggt catgacaact gaacttgcta aagaggctat tgatgctgga gctaaatttt
    13141 tggttagccc acactttgat tcagatattg ctaatttagc taatgaaaac aaggtttatt
    13201 atttcccagg ctgtgcaacg gcaacagaaa tcgtggtagc tagaaagtat aaatgtcaga
    13261 ttataaagct ttttccaggt ggagttgtgg gtccaggttt tatcaaagat attcatggtc
    13321 ctattccaga tgttgattta atgccatcag gtggcgtctc tgtttcaaat gtagttgaat
    13381 ggagaaaagc aggagcagtt gcggttggtg ttggctcagc attatctagt aaagtagcca
    13441 cagaaggcta tgatagtgtt actaaaatag ctaaacagtt tgtttctgca cttgattagt
    13501 gagtaagaga agaatatggc gtttaatact gaaacattta tgttaaaaaa tcaagcagct
    13561 atacagctgt atgaagaggt caagagacaa cctatttttg attatcattg ccacttagat
    13621 ccaaaggaca tttttgaaga tcatattttt gataatattg tggatttgtg gcttggtggt
    13681 gaccactata aatggcgtct tatgagggct aatggtattt ctgaagcaga aattacaggt
    13741 ccagcatcca accttgagaa atttaaagca tttgctagaa cactagaaag agcatacggt
    13801 aatcctgttt atcattggtc tgctatggaa ttgaaaaatg tctttggtgt taatgagatt
    13861 ttgacagaat caaatgctga ggaaatttat catcgtttaa atcatttctt gaaagagcat
    13921 aaaataagtc ctagacgttt aattgcagat agcaaagtta tgtttattgg tactacagac
    13981 caccctcttg atactctcga gtggcataaa aagttggctg ctgatgagag ttttaaaaca
    14041 gttgttgctc cgacatttag gccagatgaa gcttttattg aacatcgaca ttttgttgat
    14101 tttattacta aactgggtga cattactcaa aaagagatta cagatttttc aacatttata
    14161 gcagcaatgg aagaacgcat tgcgtatttt gctcaaaatg gttgtagggc tagcgatatc
    14221 agttttacgg aaatcgtctt tgaacaaact gatgaattgg agttgaacga tttatttaat
    14281 aaagtttgtg aaggctatat cccaaatcaa tcagaaatca gcaaatggca aacagctgtt
    14341 tttatggaac tttgccgatt atataaaaaa tatggttttg taacacaggt gcattttgga
    14401 gctttgagaa ataatcattc tactattttt gaaaaattgg gagctgacgt gggtgtggat
    14461 tcacttggtg accaagtggc tttaactgtt aatatgaacc gtttattgga ttccttggtt
    14521 aaaaaagata gtctgcctaa aatgatttgg tataatctca atccagctta caatatagca
    14581 gtagctaaca ctttggcaaa ttttcaagct aacgaacttg gagtgagatc ttaccttcaa
    14641 tttggtgcag gttggtggtt tgcagatact aaattaggga tgattagtca aatgaatgcc
    14701 ttagcagagc aaggcatgtt ggcgaatttt attggtatgt taacagattc tagaagtttt
    14761 ctatcttatc aacgtcatga ctattttcga cgtattttat gtacctactt aggagaatgg
    14821 attgaagaag gagaagttcc agaagattat caagctttag gaagtatggc taaagacatc
    14881 gcttatcaaa atgcagttaa ttattttaag aactaaaaag gaagagtgaa atcatggaaa
    14941 tgtcatttag gtggtatggt gaagacgacc ccgttacttt agaaaatatt ggtcaaattc
    15001 caacaatgaa agggattgtt acagcgatat acgatgtacc agtaggagaa gtttggtcac
    15061 gtgagcgcat tcaacagctg aaagaaaagg tagaggcagc agggcttaaa atttcggtta
    15121 tcgaatcagt acctgttcat gaagatatca aattgggacg tccaacacgt gatctcttga
    15181 ttgataatta tattcaaacc gtcaaaaatt tggctgcgga aggtattgat actatttgtt
    15241 ataattttat gccagtattt gactggacac gcactgattt agcatatcaa tatccagatg
    15301 gttccacagc tttgatattt gatgagacgg tttctaaaaa aatggatcca gtaaacggtg
    15361 aattatcttt gccaggatgg gatgccagct atagtaaaga agaaatgaag gctatcatgg
    15421 atgcctatgc tgaaattgat gaggaaaaac tttgggaaaa tctcacttat tttattaaac
    15481 gtattattcc tgaagcggaa gccgtaggcg ttaaaatggc cattcatccg gatgacccac
    15541 catattctat ttttggttta cctcgcatta ttactgggtt agaggctatt gagcgttttg
    15601 tgaaattgta cgatagtaaa agtaatggca tcactttatg tgtgggttct tacgcttctg
    15661 atccccagaa tgatgtttta gagatttcgc gacgtgcttt tgagttagat cgtgtaaact
    15721 ttgttcatgc tcgcaatatt aaattaggtg atggaaagtc atttaaagaa tcggctcatc
    15781 catcagaata cgggtcaata gacatgtatg aagttattaa gttatgccat gaatttgggt
    15841 ttgagggtgc tattcgacct gaccacgggc gtatgatttg gggagaaaca ggtcgtccag
    15901 gatatggcct atacgataga gctttaggag ccacttacgt ttctggttta tacgaagctg
    15961 tgattaaagg ttcaaaataa ggcgatgctt ccaaatttct cctgttccaa tctcctgaaa
    16021 gagaaatttg gaaagttatt atcataatat gattgtactt agctaagagt agaatactta
    16081 tagaagttaa tgagaggaat aagatgtcaa aaacaattac atttaccaat aaagttgtcg
    16141 tcataacagg tgcaggcggt gttctctgtg gttatatggc taaagaattt gcgaaagcag
    16201 gcgctaaagt ggcattatta gatttgaatc aagaagctgc tcaaacattt gctgatgaaa
    16261 ttgtagaaga aggtggtatc gctaaggctt ataaagctaa cgttttgtct aaagaaaacc
    16321 ttgaagaagt gcatcaagcc gttttagagg atcttggacc aactgatatt ttagttaatg
    16381 gtgcaggagg caacaatcct aaagctacaa ctgataatga atttcacgaa ttagacttac
    16441 cttcagaaac aaaaaccttc tttgagttag atgaagctgg tattagcttt gtttttaact
    16501 taaattattt aggaacatta ttgccaacgc aagtatttgc ccaagatatg gttggacgtg
    16561 aaggagcgaa tattattaat atctcttcaa tgaatgcttt tacccctctg accaaaatac
    16621 cagcttattc tggagctaaa gcagctatta gtaacttcac acaatggcta gctgttcatt
    16681 tctcaaaagt tggtatccgt tgtaatgcta ttgcacctgg tttcttggta acaaatcaga
    16741 accgaagctt gttatttact gaggacggtc agccaactgc gcgtgctgag aaaattctta
    16801 ataatacacc aatgggaaga tttggagaag caagtgagct gattggcgga ttgttctttt
    16861 tagcagatga aaaatcatca agttttgtta atggcgttgt ccttccgatt gatggtggat
    16921 ttgctgctta ctctggtgtt taatttacaa gataatagcc acttaacaca taagagatta
    16981 ttgagttgag tggtttttta aggaaatttg tttaatgatc ttaccactaa atgatgggta
    17041 ggttgctaat tggaaaatcc tcataagtta gataaattta gaaactggag ataatatggt
    17101 agatacaaat cacaaaatcg ctttttgcat agattctgat ggttgcgcaa tggatacaat
    17161 gacatataag catcaactct tttttggtcc aattgctgca gatatatttg ggatatcaga
    17221 acaagaaaga acaagttttt taaaagagtg ggatagagtc aatctttatt caagaactcg
    17281 tggagttaat cggtttgtgg gattggttat gggccttgag tttgccggag tagaacatat
    17341 tgataaatta aaagaatggg tcgaaacaac agactcattg tccaataatt ccttagagtt
    17401 agcgcttgcc aacgatttta gtagagattt agagaaggct ttagaatggt ccaatgaagt
    17461 gaatcgccaa atcaaaaatt atgagggaga ttctttagcg ttttcaggtg tgttagaaag
    17521 tttagaacga ttaagtcaat ttggtaatgt ttatgtcgtg agttctgcta acaaggaagc
    17581 tgtttggcaa gagtgggaag aacagggatt gctaaagtat gttgatgata tctattgtca
    17641 agatagaggt aaaaaagaag atgttatcgc cactttaatt gcaagtggtt actgccgtga
    17701 aactattttg atggtaggag attccctggg tgatctggct gcagcagaac aaaatggtat
    17761 tgcattttac ccagtacttg ttggtaagga agtcaaatca tgggaaattc ttagagaaga
    17821 tattggtgaa gcttttgcaa aaggtcaatt cgaacagcaa aggcaaaaag aatctattaa
    17881 taccttttgg gctaatttag ataactaata gaaaggatag gctatgacac atttagttga
    17941 cttaacaaag aaacccttca atctaaatca agaagcaatt gagtggattg aaaaaacaat
    18001 taatgagatg acattggatg agaaaattgg gcaacttttt ttcaatatgg gagctagtcg
    18061 ttcagaagaa tatttaacag atgttttgga tcggtatcat attgctgctg ttcgctataa
    18121 tagaggtagt tcaagtgaaa tctatgatca gaatttaatt ttacagacaa agtcgaaatt
    18181 gccgatgttg attgctgcaa atacagaagc tggtggagac ggtgctgtca cggatggaac
    18241 taaagttggc gatgaaataa aagtggctgc tacaaatgat ccgaaatatg cttatgaaat
    18301 gggaagaatc gcagggatgg aggcatctgc tgtgggttgt aatgcttcat tttctccaat
    18361 tgtagatttg acacgtaatt ggcgtaatcc tatcatagct agtcgaaatt ggggtgctaa
    18421 cgttgatcaa attatttcac tttcaaaaga gtatatgaaa ggaatcatgc aatataatat
    18481 tgtacctttc gctaaacatt ttccagggga tggcattgat gagcgtgatc atcatttgtc
    18541 atttgcttcc aatcctatga gtaaagagga gtggatgtcg acatttggcc gcatttatgg
    18601 tgaattggct gacgcaggtt taccaggagt catggcaggt cacatacatt taccaaacgt
    18661 tgaaaaagaa atgcatccag agcgagacct tgatgatatg ttaccagctt cattgaataa
    18721 aacactttta gatgagttat tgagaggaga gttaggttat aatggagcta ttgtaacgga
    18781 cgcttctcat atggttggaa tgactgcttc aatggcaagg agagatttgt tgccaacggc
    18841 gattgaagca ggttgtgatt tattcttatt ctttaatgac cctgatgaag acattcaatg
    18901 gatgaaagaa ggttacgaaa aaggtattct aacagaggaa cgtctccatg acgctttacg
    18961 tcgtactctc ggtctaaaag cgaaattagg cttgcataac tatgagggac gtcgccaaga
    19021 actctttatg cctaaagata aggctatggc tttaatcaac acattagagt cgcaaaaaat
    19081 agctgatgaa gttgctgata aagcagtaac tctggtaaaa gataaacaaa aagatatctt
    19141 tccagtaaat ccagaacgtt atcgtcatat cttacttgtt aacgttgaag gatataaggg
    19201 aggttttggt gcgatgattg ctggaaacaa gcaacgtgca tcggattaca tgaaagagtt
    19261 acttgaggca cgtggccatg aagtaactgt ctgggagtct accgaagagc gtattatgaa
    19321 attgccacaa gaggagcggg cagcagctat cgctaatgtt tacgcacaaa aacagccaat
    19381 cgcaaatcta acagaacact atgacttgat tattaacttg gtagatgtca atgctggagg
    19441 aacaacacaa cgtattattt ggccagcagc taaaggcacc cctgatcagc ctttttacgt
    19501 tcatgaaata ccatctattg tgatttcagt acaacatgca tttgctttag cagatatgcc
    19561 acaggtaggg acttatatta atgcttatga tgggcttcct agcacgataa gtgcagtagt
    19621 tgcgaaattg gctggagaat cagaatttac aggagtttca ccagtagatg cttactgtgg
    19681 tttaattgat acacatttat ggcgccaatg ctaatagaaa caagatgatg tacttttatg
    19741 gaacataaaa aaagagctta aagctctttt tttatccttc aaaatagagt agttccttgg
    19801 gtgttttggt aaatacttca aagccggttt tagtcacata accgcagtct tcaattcgca
    19861 caccaacttt atctggtata taaatacctg gttcaacaga gaagcacatg ccttcttgaa
    19921 tctccatatc gttaccagcc ataatagatg gaaattcatg gacatccata cctaatccat
    19981 gaccaaggcg gtggttaaag tattgtccat aaccagcctt ttcaataaca tttcttgctg
    20041 ctgcgtcaac ttcagaagca aggactcctg gcttaataaa atcaatagcc gcttgatgtg
    20101 cttcaagaca taaatggtaa atatcttttt tgaattgatc aggtttcccc actgctactg
    20161 tacgggtcat atctgacgta taacctagtg tttctacacc taaatcaaat aataacaaag
    20221 cattattttc gattttatta gttcctggga taccatgtgg atttgctgca ttattgccag
    20281 tcaaaaccat agtgtcgaaa ctcattttat tgataccttg ttttttcatt tcaaattcga
    20341 tttgagcgat aatatcggtt tcagtgttgt ttagcgagat attgtcaaat ccaacttgaa
    20401 cagctttatc agcaaattct ccagcgacta acattttctc aatttcatct cgtgatttaa
    20461 ttaggcgcat gttttgaata taaggtgtta ggttttcaaa atgaccatca aaaacggttt
    20521 ggagaccttg gaatttagta acattaagat tatcaaattc ggctagaact ttagaaacag
    20581 aaaaggatgg taagttgcta gctatttttt gccaaggatt ttctgaatct atgtaaccaa
    20641 agacgggaaa tggtacactt tgttttgcac gcgatacttc tagtgctggt acaaataaaa
    20701 taggatcgcg atcttcatag acaaataaaa acatttgtct ttcatgggga tcgcaaaaga
    20761 aacctgttaa atagttgact gtcacaggat cagaaaagac agcaagttca gcttggactg
    20821 aatgtaagtg atggcgtata cgattaagtt ttgacataat aacccttctt cctaaaagga
    20881 acttagtcta gttaggctag tttcttatgc ctattatttt tccaaaaaaa taggaaaaaa
    20941 tcaagtttag ttagtatttt gaaagttttt cataaaaaag ctttataaaa gcttgaaagt
    21001 gtttccaaaa ggtgataaaa tatcagtgaa agcgtcattt tcaaatagaa aggaagttaa
    21061 atcaatatga atacagatga tacgattacg atttatgatg ttgcccgtga agcaggtgtt
    21121 tcgatggcaa ctgttagtcg tgtggtaaat ggaaataaaa atgtcaagga aaatacacgt
    21181 aagaaagtcc ttgaagttat tgatcgtctt gactatcgtc caaatgctgt ggcgcgtggt
    21241 ttagcaagta aaaaaacgac aactgttggt gttgtcattc ctaatatcgc aaatagctac
    21301 ttttcaattt tagcgcgtgg tattgatgat attgcagcaa tgtacaaata taacattgtc
    21361 cttgcttcaa gtgatgaaga cgatgataaa gaggttaatg ttgttaatac tttgtttgct
    21421 aagcaagtag acggtattat ttttatggga caccacttaa ctgaaaagat tcgtgcagag
    21481 ttctctcgtt cacgcacacc gattgtttta gcaggtactg tggaccttga gcatcaatta
    21541 ccaagtgtta atatcgatta caaagcagca gcagttgatg ttattgatat tttagctggc
    21601 aatcataaag acattgcttt tgtatcagga ccacttattg acgatattaa tggcaaggtt
    21661 cgtttggcag gatacaaaga aggtctcaag aaaaatggct taaactttaa agaggggttg
    21721 gtttttgaag ccaactaccg ttatgctgaa ggctttgcat tagctcaacg cgtgattaat
    21781 gctggagcaa cagcagcata tgttgctgag gatgagttag cggcaggttt gttaaatggt
    21841 ctttttgagg caggtaaacg tgttcctgaa gattttgaaa ttatcacaag caatgactcg
    21901 ccaatcgcac aatatacacg tcctaactta acttctatca gtcaaccggt ctatgatttg
    21961 ggtgctgtta gcatgcgtat gctaacaaaa attatgcaca aagaagagtt ggaagaaaaa
    22021 gaaatagtgc ttaatcacgg gattgttaaa cgtggcacaa caaataatta gtgttagagt
    22081 atgttatttg aaaaatagtg gtagaatggt agtagcttag cgcttaactt tctgatactt
    22141 aacatgatat attgagagga ttagggtagc ttagcttcct aatctttttt tagaggtgta
    22201 atatgacaaa tgaattgata atgcaagctt ttgagtggta tttacctagt gatgggaatc
    22261 actggaagaa attagaggag tctatatcag accttaaaaa acttggaatt agtaaaatct
    22321 ggttaccacc agcatttaag ggaactagca gtgatgatgt aggatatggt gtttatgatc
    22381 tctttgattt aggagaattt gaccagaatg gaacaattag aacaaaatat ggtaggaaag
    22441 aagagtatct aaagcttatt aagtcgttaa aggcaaatgg cattaaaccg tttgcagata
    22501 tcgttcttaa ccataaagcc aatggtgatc ataaagaaaa atttcaagtc atcaaagtca
    22561 atcctgaaaa tcgtcaagaa gcattaagtg aaccctatga gattgaagga tggacgggat
    22621 ttgatttccc aggtagacag ggtgagtaca atgattttaa atggcactgg taccacttta
    22681 ctggtttaga ttacgatgct aaaaataatg aaacagacat ctttatgatt gtaggcgata
    22741 ataaaggttg ggcagacgat gatttgattg atgatgaaaa tggtaatttc gattatctca
    22801 tgtacaatga cattgatttt aagcatcctg aagttataaa aaacttacaa gattgggcta
    22861 agtggtttat tgaaactaca ggcattgaag gttttcgttt ggatgctgtg aaacatatcg
    22921 attcttactt tattcaaact ttcattaatg atattcgtac aaaaataaaa cccgatttag
    22981 aggtctttgg cgaatattgg aaatctgacc aaacatcaat gaaggactat ttagaggcaa
    23041 cacaatttca attttctctt gttgatgtta ccttacatat gaattttttt gatgccagtc
    23101 accaaaaccg tgattttgat atgcgaacaa tttttgatga tagtcttgtt attgataatc
    23161 ctgagtatgc tgttacattc gtagaaaatc atgatacgca aagtggacaa gctctagaaa
    23221 gtagagtgga agattggttt aaacctttag cttatggctt gatactttta cgccaacaag
    23281 ggactccatg tcttttttat ggtgattact atggcattca aggagaattt ggtcaaccat
    23341 cctttaaaga ggttattgat aaaatggctg agctacgcca aaactatgta tttggcaaac
    23401 aagtcgatta ttttacgcat tcaaattgta ttggttggac ttgtcttggg gatgaggaac
    23461 ataattcttg tttagctgtg gtcctaacaa atggtgatca gggatggaaa catatggaag
    23521 taggggaaat ttatgcaggc aaaacgtttg ttgattattt aggtaattgt gaacaagagg
    23581 ttgttatagg agatgatggt tggggtgatt ttttagttga atctgcgtct atctcggctt
    23641 gggttcctaa aatagaggaa gctaactgat aaatcaggtg aaaaaaaagg taaaatgaga
    23701 tataataaga cctatgaaag ttttactgta tttagaggct gaagaatact tgaaaaagtc
    23761 aggtatcggt cgtgctatca aacatcaaga gaaagctctt caaattgctg ggatagatta
    23821 taccactaat cctacagatg actttgacct tgttcatatg aatacctatg gtattagaag
    23881 ttggcttttg atgagtaaag ctaaaaaaac tgggaaaaaa gtaattatgc atggccattc
    23941 cactgaagag gactttagaa attcttttat tggctcaaat ttagtatccc cgctttttaa
    24001 atggtacctt tgccgttttt atcaaaaagc agatgccatt ataaccccaa ctgattattc
    24061 taaacaacta ataaaagcat atggcatcaa gaaaccaatt tttgttttat ctaacgggat
    24121 tgatctaagt cgctatcaga ggtcagaaaa gaaagaatct gcatttcgtc actattttca
    24181 cttaagtaag gatgataaag ttgttatggg ggccggtctt tatttcatgc gtaagggaat
    24241 tgaccaattt gttgaagtag ctgcaaaaat gcctgatatt cgttttattt ggtttggaga
    24301 aacaaataaa tgggttattc ctcgaaaggt taggcaaatc gttaccaagc aacatccctc
    24361 aaatgttact tttgctggtt acataaaagg tgatgtttat gaaggtgcca tgagtgctag
    24421 cgatgctttc ttctttccga gtcgtgaaga gacagaaggg atagtagttt tagaggcttt
    24481 agctagtcat cagcatgtgg ttttaagaga tatccctgtg tatcacggtt gggtgaccga
    24541 agacagtgtt gaattagcga ctgatgttga tggttttgtt gaaaagttgg ataaagtctt
    24601 atctggtaag agtgataaga ttaaagaagg ttatcatgtg gctgagagtc gaagtatcga
    24661 acgaattgcc catgaattgg caagtgttta tcaaaaagta atggagttgt aagatgcgta
    24721 taggtctatt tacagataca tacttccccc aagtttcggg agtttcaaca agtatccgca
    24781 ctttaaagga agggttggag aaggaaggac acgaagttta tattttcaca actaccgacc
    24841 gtaatgtcaa acgttttgaa gaccctacaa ttattcgttt accaagtgtc ccttttattt
    24901 cttttacgga tcgtcgagta gtttatagag gtttaatttc tgcttatagg atagctaaag
    24961 actacgaact tgatattatt catactcaaa cagaattcag cttagggctt ttaggaaaat
    25021 tagttgcaaa agctttaaga atacctgttg tgcatactta tcatactcaa tatgaggatt
    25081 acgttggtta tatcgcaaaa ggcaaattga ttaagccaag tatggtcaaa tatatcatgc
    25141 gtacctacct aagtgatttg gatggtgtca tatgtccttc tcgaattgtt ctcaatcttc
    25201 tagatggtta cggtgtcaaa attccaaaac aagttattcc gacaggtatt cctgttgaga
    25261 actatcgtcg agaggatatc tctgaagaaa caatcaagaa tctacgaacg gagcttggac
    25321 ttgcggataa tgatacaatg ctattgagtt tatcacgggt atcttttgaa aaaaatattc
    25381 aagcaatctt gatgcattta tcagctgtcg ttgatgaaaa tcctcacgta aaattagtga
    25441 tagttggaga tggtccttat ctttcagatc taaaagaact tgttcactct ttagaacttg
    25501 aaaattcagt tatttttaca ggtatggttg agcatagcca agtggctatc tactataaag
    25561 catgtgattt ctttattagt gcttcaacaa gtgaaacgca aggattaact tacattgaga
    25621 gtctggcgag tggacgtcca atcattgcgc aaagtaatcc ttatctagat gatgttattt
    25681 cagataaaat gtttggaact ctgtacaaga aggaaagtga tttggcagat gctatcctag
    25741 atgcaatagc agagacaccc aaaatgactc aagaagctta tgaacagaaa ttatatgaga
    25801 tttctgctga gaatttcagc aagtctgtat atgcttttta cctagacttt ctgatttcac
    25861 aaaaagctag tgtgaaagaa aaagtctctc tgacaatagg aaataaggat agccattcta
    25921 ctcttcgttt tgtcagaaaa gcggtatatt tacctaagaa agtttttacc tttacaggaa
    25981 gagcctctaa aaaagtggta aaagcaccta aacgtcgtat ttcatctatt cgagattttt
    26041 tagattgaga cgaataaaat tagtaaatgt cttgaaatta taaaaaatat tgttataata
    26101 aaaaatgaag acatgttagg ttctaataga ttttaagagc gagcactggt tggtggaaag
    26161 agtgcaatta gagaacttag tactacttcc ttgattttat gcaaacataa aagggtggtt
    26221 cccttatcgc cactagaggt ctgacacaag tatactcgag tttttgtgtt ggataaattg
    26281 aggtggaacc acgtagtctt tacgtccttt tggatgtaaa ggctcttttt ttataaaaga
    26341 agtggttgca taaaagaggc agattgcttt gctaatgtta tacttattat ccctagctag
    26401 gataatgtgg aattcaatca aaggtcaatc cctttgttta ttagtgaatt aatgtgttta
    26461 ggaattggta tcatcctaac agttattaag aaaaggagaa caagatgatt aaaatcactt
    26521 tcccagatgg tgcaattcgt gaatttgaat ctggcatcac aacatttgaa attgcacaat
    26581 caatttctaa ctctttagct aaaaaagctt tagctggtaa atttaatggt caacttattg
    26641 atacaacacg tgctatcgaa gaagatggta gtattgagat tgtaacacct gaccacgaag
    26701 atgctttagg agttcttcgt cactcagcag cgcatttatt tgctcaagct gcaaaacgcc
    26761 tttttccaga cttatgttta ggtgtaggtc cagctattca agatggtttt tactacgata
    26821 ctgataataa atcaggtcaa atttcaaatg atgatcttcc tcgtattgaa gaagaaatga
    26881 aaaaaattgt taaggaaaat cacccttgta ttcgtgaaga aatcagtaaa gaagaagcgt
    26941 tagaactttt taaagatgac ccttataaag ttgaattaat tagtgaacat gctgaagatg
    27001 gtttaactgt ttatcgtcaa ggtgaatttg tagacctttg tcgtggacct catgtcccat
    27061 caacaggacg tattcaagtc tttcatcttc taaatgtcgc tggtgcttac tggcgtggta
    27121 acagtgataa tgctatgatg cagcgtgtct atggtactgc ttggtttgat aaaaaagatt
    27181 tgaaagcata tcttaaacgc cgtgaagaag ctaaggaacg tgaccatcgc aaattaggta
    27241 aagaacttga tttatttatg gttaatcctg aagtaggtca aggattgcca ttctggttgc
    27301 caaatggagc aactattcgc cgcgaattgg aacgttacat tgttgataag gagattgctt
    27361 caggttatca acacgtttac acacctccga tggcatctgt tgaattttac aagacttctg
    27421 ggcactggga ccactatcgt gaagatatgt tcccaacaat ggatatggga gatggagagg
    27481 aattcgttct tcgtccaatg aactgcccac accatattga agtttataaa catcatgttc
    27541 attcatatcg tgaattgcca attcgtatcg ctgaacttgg tatgatgcac cgttatgaaa
    27601 aatcaggggc cctcacagga ttgcaacgtg tacgcgaaat gacactcaac gatgcccata
    27661 ttttcgttac acctgagcaa attaaagatg aatttcttaa agctcttaat cttatagcag
    27721 aaatttatga agattttaac ttaacagatt accgtttccg cttatcttat cgtgacccag
    27781 aagataaaca taagtattat gataacgatg agatgtggga aaatgcacaa gctatgctaa
    27841 aagaggctat ggatgatttt ggattagatt attttgaagc agaaggcgaa gcagcctttt
    27901 acggtcctaa attggatatc caggtgaaaa ctgctcttgg taatgaagaa acactatcta
    27961 ctatccaact agatttcctt cttccagaac gctttgatct caaatacatc ggtgcagatg
    28021 gtgaagaaca tcgtccaatt atgattcacc gcggtggtat ttcaacaatg gagcgcttta
    28081 cagctatttt aattgaaact tacaaaggtg cctttccaac ttggcttgct ccacaacaag
    28141 tttctgttat tccaatttca aatgaagccc acattgatta tgcttgggaa gtggctcgtg
    28201 tccttaaaga tcgtggtatt cgtgctgaag tagatgaccg taacgaaaag atgcaatata
    28261 agattcgtgc ggctcaaacc caaaaaattc catatcaatt gattgttggt gataaggaaa
    28321 tggaagaaaa agccgtaaat gtgcgccgct atggaagcaa agcgactgaa acaaaatcta
    28381 tagaggaatt tgtagaaagt attttagctg atattgcaag aaaatctaga cctgacgaag
    28441 ttaaataaat tttattagtc attataaaaa tcattaataa atgatgtgaa ctgattactg
    28501 tatgatgcag aatcagtttt ttagatgcta attttttctt tatgtttaat tataagctaa
    28561 ttatagataa ataatctcat ataacattca caaaattaga tttttttggt ataattaata
    28621 tgattgacac ataaaggaaa atagtattaa taaaaagggg tatggagcat tgcgaacagt
    28681 attagtagtg caaggtgatg acgaaacaat agagttatta aggtcgtatt tagagggagc
    28741 tctttataag gttgtgatgg ctagtgatgg tgaggaggct ttttctttat ttcagcaaca
    28801 tcagattgat ttagcaatca tagatattac gctacctaaa atagatggat atgagttgac
    28861 gcgactcatt cgtcaagata gtcagattcc tattattatg ctagcagcta agacaacaga
    28921 tatggacaga atattaggat tgaatattgg agcagatgat tttatcacga aaccgtttaa
    28981 ttctttggag gttttagctc gaattaattc gcaactgcgt cgttactatg aattcaatag
    29041 tcttgccaaa cctaaaaatc aatttataaa gataggggaa ttggaattag atgaggaaca
    29101 tgttgaacta actaaaaatg gtaaacatat caagttgact gctacagaat ttaagattct
    29161 tcatatctta atgtcatcac caggaaggat ttatactaaa acacaactct atgagaagat
    29221 taatggtagg tatttagaag gtgatgagac aacgattatg gtgcatattt ctaatatccg
    29281 tgacaaaatc gaagatgata gtaaatatcc taaatatata aaaacactta gaggagtagg
    29341 atataaaatc gaaaaaccgc agtatcttgt ttaaaaatat aatggctagt aagagccatt
    29401 ttttagtgaa gatatttctt ggttttaagc tttccttttt gctataataa gtaaaattgg
    29461 aggggaggga tatggcaaag accgctctta atattccttt tacagttgaa gggatagaaa
    29521 attattatca aaatataaaa atgatactgt ctttagcaga taaatggagc atacctaaag
    29581 atgatctatt acttttttta aagggcctca aggctattga tgatataaaa gttggctctc
    29641 ctatttttaa gtatttttgg acttctttaa gtttacaagc acccctcaaa gctttagaat
    29701 tcgtgttaga gcaggctaaa atgccaactg aattgtcagg agaattatca gaaacacaat
    29761 acttggtcgc acagttttca gatgagttag ctccacatga tgatttctgg atagccttat
    29821 cacaagttat ttatgattct tttcctggga atagtctagc cgaagatact gtattaaata
    29881 ggaaactcca tcaattccgc tatttgattt ctagtcagca agcacagtat gttcgacgat
    29941 actttaaaga tgttggtatg actgatagag atgctttagt aaactaccta tcatgtttaa
    30001 gagaaccaga tagtatcgcc tactatgaat ctgctcgctt gcataataaa cgtcggagaa
    30061 atggtgaaat ctttggtttc ccagatgatg agccggttat taatagtaag cttttaattt
    30121 cattccatac ggaatttatt attgatgaca aaggaaactt tttaaacgag attgacgcag
    30181 aagtaataac acgtaatggt attatcaatg gagctagctt taattatgct tttaaaaata
    30241 atacacgtca taaagaatta gatgttgatc ctgtaaagtt agacccaaaa tttcgaaacg
    30301 atatgactag aggttataga tcgccaaatc tatctagaag gaaatggttt ttctttaaag
    30361 aagaggatta cgattgtagt tatttcaata aaaaaggata ttacgcattt ggaaggcgta
    30421 gtgctaagca atcggttgat aaacaagtca aatacttgaa gaaggctgtg cagaaaatgc
    30481 gcatcaactg atacatatac
//
LOCUS       DATRSD010000012        29295 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRSD010000012 DATRSD010000000
VERSION     DATRSD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29295
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.012"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(39..236)
                     /locus_tag="VBD85_000450"
     CDS             complement(39..236)
                     /locus_tag="VBD85_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133024.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(250..1983)
                     /locus_tag="VBD85_000451"
     CDS             complement(250..1983)
                     /locus_tag="VBD85_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133025.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNQLPVAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVVFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELMAQK
                     GIYYQMQNAQK"
     gene            complement(1984..3702)
                     /locus_tag="VBD85_000452"
     CDS             complement(1984..3702)
                     /locus_tag="VBD85_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133026.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFQIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     VMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRTLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAITKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEE"
     gene            complement(3714..4316)
                     /locus_tag="VBD85_000453"
     CDS             complement(3714..4316)
                     /locus_tag="VBD85_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7133027.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(4318..5295)
                     /locus_tag="VBD85_000454"
     CDS             complement(4318..5295)
                     /locus_tag="VBD85_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO7133028.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGPVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(5300..6556)
                     /locus_tag="VBD85_000455"
     CDS             complement(5300..6556)
                     /locus_tag="VBD85_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO7133029.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKTLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(6648..7244)
                     /locus_tag="VBD85_000456"
     CDS             complement(6648..7244)
                     /locus_tag="VBD85_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO7133030.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(7237..8067)
                     /gene="prmC"
                     /locus_tag="VBD85_000457"
     CDS             complement(7237..8067)
                     /gene="prmC"
                     /locus_tag="VBD85_000457"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO7133031.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(8067..9146)
                     /gene="prfA"
                     /locus_tag="VBD85_000458"
     CDS             complement(8067..9146)
                     /gene="prfA"
                     /locus_tag="VBD85_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO7133032.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKIDEVIDALVMYDQTQKLEALNK"
     gene            complement(9181..9750)
                     /locus_tag="VBD85_000459"
     CDS             complement(9181..9750)
                     /locus_tag="VBD85_000459"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO7133033.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            9888..10070
                     /locus_tag="VBD85_000460"
     CDS             9888..10070
                     /locus_tag="VBD85_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO7133034.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            10219..11157
                     /locus_tag="VBD85_000461"
     CDS             10219..11157
                     /locus_tag="VBD85_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO7133035.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIEVLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEISEAMILSNPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            11175..11777
                     /locus_tag="VBD85_000462"
     CDS             11175..11777
                     /locus_tag="VBD85_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7133036.1"
                     /translation="MKLIGIVGTNSNQSTNRQLLQYMQQHFADKAKIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDANFDDFRLFVKITGKLSNTRDLLQKEAENFDWESL"
     gene            11801..13036
                     /locus_tag="VBD85_000463"
     CDS             11801..13036
                     /locus_tag="VBD85_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7133037.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQDQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITGIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TGDAPVNQATDATSGASEH"
     gene            13135..13923
                     /locus_tag="VBD85_000464"
     CDS             13135..13923
                     /locus_tag="VBD85_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO7133038.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AMTILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(14018..15292)
                     /locus_tag="VBD85_000465"
     CDS             complement(14018..15292)
                     /locus_tag="VBD85_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO7133039.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGVFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVADAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLGGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNGTNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(15292..15873)
                     /locus_tag="VBD85_000466"
     CDS             complement(15292..15873)
                     /locus_tag="VBD85_000466"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO7133040.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVDFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(15977..16072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(16128..17111)
                     /gene="guaC"
                     /locus_tag="VBD85_000467"
     CDS             complement(16128..17111)
                     /gene="guaC"
                     /locus_tag="VBD85_000467"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO7133041.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVDGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(17314..18654)
                     /locus_tag="VBD85_000468"
     CDS             complement(17314..18654)
                     /locus_tag="VBD85_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7133042.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLSGVLIDSFAPNLPILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKSVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            18946..19617
                     /locus_tag="VBD85_000469"
     CDS             18946..19617
                     /locus_tag="VBD85_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133043.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKIEANDIKTFSELSKTMTEFHYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPSAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(19688..21688)
                     /locus_tag="VBD85_000470"
     CDS             complement(19688..21688)
                     /locus_tag="VBD85_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO7133044.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGFLNSFAHPEVFKAINPYYGLKLLFSLENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSIKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(21823..22584)
                     /locus_tag="VBD85_000471"
     CDS             complement(21823..22584)
                     /locus_tag="VBD85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO7133045.1"
                     /translation="MISRKVALVTGASAGFGAAIVTNLVSDGYSVIGCARRMDKLKCL
                     GEKFSEGHFYPLQMDITSRESVDKALESLPKNLQSIDLLVNNAGLALGLDKSYEADFG
                     DWMTMINTNVVGLIYLTRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGASKAFVK
                     QFSLNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDIA
                     NTVSWVASQPEHININRIEIMPVSQTYGPQPVYRD"
     gene            complement(22646..23638)
                     /gene="pta"
                     /locus_tag="VBD85_000472"
     CDS             complement(22646..23638)
                     /gene="pta"
                     /locus_tag="VBD85_000472"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO7133046.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDIDPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(23664..24548)
                     /locus_tag="VBD85_000473"
     CDS             complement(23664..24548)
                     /locus_tag="VBD85_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7133047.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(24545..25381)
                     /locus_tag="VBD85_000474"
     CDS             complement(24545..25381)
                     /locus_tag="VBD85_000474"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO7133048.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDEGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(25356..26027)
                     /locus_tag="VBD85_000475"
     CDS             complement(25356..26027)
                     /locus_tag="VBD85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7133049.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            26137..26709
                     /locus_tag="VBD85_000476"
     CDS             26137..26709
                     /locus_tag="VBD85_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO7133050.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            26886..27860
                     /locus_tag="VBD85_000477"
     CDS             26886..27860
                     /locus_tag="VBD85_000477"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7133051.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            27864..28979
                     /locus_tag="VBD85_000478"
     CDS             27864..28979
                     /locus_tag="VBD85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7133052.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            28981..>29295
                     /locus_tag="VBD85_000479"
     CDS             28981..>29295
                     /locus_tag="VBD85_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO7133053.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLV"
BASE COUNT         9696 a         5669 c         4600 g         9330 t
ORIGIN      
        1 tagctttttt atatctatct aacgatgttc tattattttc aatcatgaaa tataactgca
       61 attaacttca cgatcaaata atagagcaga cttacaatta accacgttat cccaataact
      121 gcaactaaac ctattaaaac agataatacc gtattcttag ttgctagttg gttactgata
      181 aaccatgaaa ctgcagaaat aaatatcagt aataatagtt gaagcctttt tttcatataa
      241 ttcctcttac tacttttgtg cattttgcat ttgataataa ataccttttt gagccattaa
      301 ttcagagtgg ttacccactt ctacaatttc accagatacc ataactaaaa taatatctgc
      361 tgtttgaatg gtagataagc gatgtgcaat aataaagctt gttctcccct tcatcaacat
      421 ttggaatgct tcttgaacaa gaacttcagt acgagtatct atagacgatg tcgcctcatc
      481 taatatcaat attctaggta atttaagaaa aatacgagca attgtcaata gctgacattg
      541 cccctgtgat agcgaatctc cagcatcttc taaatacgta tcatatccat tcggtaattg
      601 tttgataaag aaatctgcat tagctgcttt tgcagcctct atcacttctt cacggctagc
      661 tttaggattg gcatatgcaa tgttatcgtg aatcgtagca ctttttaacc acgtttcttg
      721 taataccatt ccaatttctt gccttaattg tgatggctca taatcagtaa taggcttacc
      781 atctaaaagg atatttcccc cgtcaacttc atagaaacgc attaataaat tgattaaagt
      841 agacttccca gcacctgttg gtcctacaat agcgacctta gctcctgctg gaatgtgtaa
      901 attaatacca ttcaatagtg gttttgattt attataacca aaaacaacat ttttaaaatc
      961 gatttgaccc ttgaccgttg atgaatcaag tttttctgtc cctgttatat ttggtgacga
     1021 ttcttctaaa atagagtaaa ggcgttctgc acaagccaga gcactttgca tctcagacag
     1081 gacggaagag atgtcattaa atggcttagt atattgattg acataattta agaaagtgat
     1141 taattgacca acactaaaag cccctgacat aatacgaagg gcaccaatcc cagctaagaa
     1201 gccataaatc aaactattga taaatcgggt tgatggattg acagttgaag aataaaaaat
     1261 tgctgattga gaaaagttag catactcttg attaatagtc ctaaaatgat cactactttc
     1321 ttcttgcgca ctgaatgctt gaataaggct ttcttgactc accatctctt caatgaattg
     1381 agtttgtcgt cctcgtgatg ccgtttgatt ttgatataag tgataactct ttttagcaat
     1441 aaagcgggct agaaagagtg ataaaggcgt cagaaataat accaaaaaaa gcattaataa
     1501 atcaatcttt gccatactaa aaatagtaac tagaatagtt aataaaccaa caaagaactg
     1561 gttaaaaacc attaataaac catttgataa ttgctccgta tcggtagtca cacgactaat
     1621 caagtcacct atgccacgtt tatcaaggta agctacaggt aattgattca acttttccat
     1681 tacagccttt cttaaagaag caacataatg aaaaattaag cggttataga gcagaggatt
     1741 aatccattga ataatagtat ttgctaaaat aactgcaacc atcttccaca ttataggtaa
     1801 caataatatc atagaatgag gcgataatac aacgtctacc gcttgtccaa ttagaacagg
     1861 caaataaaca gtgaggcaaa cttgtactac cgtccctaat aatgccattc ctactaagct
     1921 ttttttagaa agaagatctt gaacaagctt tttttgtatc tttttaggat gacttgcttt
     1981 catttactcc tccttaaaat tttgtgaatg gtaaatggac ttataaactt tgttatgagc
     2041 taataaagat tgatgattat caaaaccaac ttgtttcccc ttatcaagga gtaagattct
     2101 atctgcattt tgtatactat taattctctg tgatacaata ataagattag tttggttgaa
     2161 atgtttagtt atggctttaa ataaacgagc ttctgttaaa taatccaatg cagatgttgc
     2221 atcgtctaaa atcaaaaatg gtatcttatc ttgaactaaa gctcttgcaa tcgtcaacct
     2281 ttgcctttga ccaccagaga aattcctacc aaaactctct acaggggcat caagttgacc
     2341 atctttttct tttacaaaat cactagcttg tgctatttct aaagcagtcc aaagtttttc
     2401 ctcactaact ttaccaagtc ccaaagaaag gttagaacga atagttcctt taaacaactg
     2461 agcattttga ggaacaactc ttaccagggt acgccaattt gaaattgtat ctggactttt
     2521 tccctgatga tatatatcaa tatccccttc ttgcacttta taaatatgaa gcagtaaatt
     2581 aaccaaggtt gatttcccag agccagttcc accaattatc cctaaagttt ctcctacatt
     2641 catagaaaaa gtaatatcag ataaagcttt ttcagaggaa tttggataag aaaatgccat
     2701 ttcttgtact tctaaagcct tattcgaata ttttggctca agtttgtcat caattatttc
     2761 agacggtctt tcaaaaacgg ctataattcg cttagcactg atataacttt ggttcagtga
     2821 tgtcaccaac attgtcattt ttaacaattc aacaagaatt tgcaataagt aattaattaa
     2881 agcaaccaac attccttgtg ataaaagatg attagctata tttaaattac cacgccaaat
     2941 aatcacaacc aaagtgatat taacaactag aaatgttaga ggcgttacta aactagaaag
     3001 cctacctgct tttaattgta aattcgtata atgataattg atatcattaa atgcttcact
     3061 ctctctatct acttgattaa aggcacggat aactcgtaca ccttgaagtt gttgcctagt
     3121 caactttacc aaataatcgg ttgaagttct gattttaagg taaataggat ttaatagtcg
     3181 agacattaca aaaacaatga taaataacgt gacaaccatt accaagaacc aaatagtcaa
     3241 tgaggggcta atggaaaaag ccataattat ggcaccaaaa acaataatcg gagccctcaa
     3301 aaataaacgt aaaaattgat ttaaaccagt ttggatttga aatgtatcag cagtcaaacg
     3361 agtaatcaag ctagcagttc ccaattcatc tctgtctttt ttacccagcg acatgacttt
     3421 ttgatagaga tcctctgtca attgtctcgt ataaccaact gcggcttttg aagaataata
     3481 ttgagcagtt atcgctacta caacacccac cgcagccaaa aagaaaatca atccaatttg
     3541 caacagtaaa ctacttctat cccctcgtgg aatggtctcg tcaatcattt ttgcaataac
     3601 gattggtacc aaaagttcaa aagaggcttc taataatttg aaaaggggtc caaagatagt
     3661 ttctttgata taccccttaa aataataagt tagtcttttc atacttaccc ttcttaaaac
     3721 aactccatta tttttatcaa ataaaggttg aattgaactt cacgtgagta gtaaatatta
     3781 ccaccatttt tatagagctt ccctcctgaa attaaggcga gaggatgata gtaaaagtaa
     3841 gtgtccccgt tataatttcc taggctggga gctacaacat ttttagagta atttttggcc
     3901 aactcaatgg aattttcacc cccatgatcc gctacataat caatataaga agaaccaaaa
     3961 ttatatgctt ggacagcagt ccatacatca accttagctt ttttagcttg acttatattt
     4021 tgtgacaaca gagtgacgcc atgtttaata ctggtgtgac tatccgtaat actgttcgta
     4081 gttccactga tactttcact tgattgcatc acatctatag catctccctt agtttcagta
     4141 taaatcatag ataatactaa gtgcacatta gcctctgaac cattctctgc aagagtattc
     4201 tgcactaaat catgatattg aagcactttt ttaacatttt catggatgat aactagacgg
     4261 tacccaatga aaataataat aattaatgct atcaaacgtt ttaaaaattt aaacatatta
     4321 tttattttga atatcctgta tgtttttgat tagtattgag tatgtaccat cagggttttg
     4381 gataaattca actgattcag catcttggta gacattgttg ggtacaatca attcaatacc
     4441 atttgaaaga gatagcttct gattctctaa cttcttaatt tgacgggaat gatcaatatc
     4501 acttacttga acaggtccag gtattgcgtc tttcaattca tcaacaaaag tcagcctggc
     4561 tgttaaatta tcatcaaata attgatctgc taatttttct ggagacaatt cttgctcttc
     4621 ttctaaattc ttataaatag ccgatttcat ttttgattgg aaagaaaaat catctttgtt
     4681 gaaattttca gcaatttttt gagctgtctg ttcaaccatt ttgatagatt ttttaacaga
     4741 ctgttctggt tgtacttgta ataaattttc agaaaaataa ttagcaaatg aaccattgtg
     4801 tttgatacgc ttttcaatta aatagtattg ctttgatgac ttgttaacaa ccaaagcttc
     4861 atctggagtt tgagcagcac ttggaaggtt attctgtgtt attgtaattg gctgctcttg
     4921 attttcagaa acatgagcaa attgttcctt taatgagatt cgaaggaaag caaaatgttc
     4981 cattccatcc ttgtcaaatt gaataaaaac taagtcattc gttttttgat cttccgatat
     5041 gacaaactct tctttccaaa gttgcgcaat ttgacaggaa gaaacatata aatcatcttg
     5101 taaatgactc ataaatacat tatcctctcc aaaatagcca cgcttagctt catcagaaaa
     5161 aactttactc aattttttac gaaagtagtc atcaatcctt ggagttaggg ttaacggagt
     5221 atctgatagc actaactctg tgtcgttagg agaaaactga tgtataataa tctgtttgat
     5281 ataaaaatcc atcataacat taattctcat acaaagggaa agcgtccgtt aatgatttaa
     5341 tttcttgacg aacttctgtg aggacatctt gattttcatg atttttgagt gccttaatca
     5401 ttaattcagc gattcttcga gattcttcaa ctcccattcc tcgactagtg attgctggag
     5461 taccaattcg aatacctgat gttttaaatg gtgatagtcg ctcaaacggt atagagttct
     5521 tgttaagagt gatattgact tcttcaagaa cattttgtgc ctttttgcca ttctcaataa
     5581 cttttgtgac gtctactaag aacaaatgat tatcagtacc atcagaaatc aaatgaaagt
     5641 catcatcttc tttaaaaact ttagccatgg cttgagcatt tttaataata tcctcaccgt
     5701 atattttaaa ggaaggatct agggcttctt tcaaagctac agcttttgca gcaattacat
     5761 gttctaacgg tccaccttgg agtcctggaa agactgcaga attaattttt ttagcaattg
     5821 cttcatcatt ggttaaaatc aaaccacctc taggtccacg tagagtctta tgggttgtcg
     5881 ttgtcgttac atgagcataa ggaataggac tagggtgatg accagaagca actaaacctg
     5941 caatgtgagc catatcaacc attaagtaag catctactgc gtctgctatt tgacgaaatt
     6001 tttcaaaatc aataatgcga gaataggccg acgcccctgc aacaattaat ttaggttgcg
     6061 tttcctgtgc tattttcaaa atgttatcat agtctagcat ctccgtctta ggatcaacag
     6121 agtaagaaac aaaatgataa gtttttcctg agaagctaac tgaagcgcca tgtgttaagt
     6181 gcccacctgc tgctaagtcc attcctaaga cagtatctcc cggctcaatt aaagccatgt
     6241 aagctgctgc attagcctga ctaccagaat gtggttgaac attagcaaac tcagcattaa
     6301 ataacgtctt agctcgttct atagctaaac tttcaactac atcaacacaa tctgttcctc
     6361 cataataacg atgactagga tacccttcgg catatttatt tgtcaaaaca gaaccttgtg
     6421 ctgccataac agcttttgaa acaacgtttt ctgatgcaat taattcaata ttattttgtt
     6481 ggcgaatttc ctcatcatga atagcctgcc aaagttcttg gtcaaattct ttaaaattgt
     6541 ctttatcaaa aatcattttt tactccttaa gctcgttata acttttaaat aatggtttca
     6601 atcattatat ttatcacatt tttactatta gttgcaagat gattaaatta attttcaaaa
     6661 attaattcag gtatcgtagc cgttagtacc tcttgcgtga ttgcaccttg ccttaaaatc
     6721 ttaactctat caccagacaa atcaataata gtagaatcca cacctgtcaa tgctttatca
     6781 tcttcaatac ctaaaacttg atgattaaac tgtttttgaa tttctgagaa tacacgacca
     6841 ctatcacatc cagaaatatt agcagaagga ccaatcaatg gaccatggtt agcaatcata
     6901 tcaagggtta cagggtgctt aggcatccga aatcctacgc ttgataaccc agaattgatc
     6961 caatccggaa cttcttgtga agcttccaaa ataatggtta gcggtcctgg taaaaaagca
     7021 tcgtacaatt gcttcaaata ggtcggttga tttttagaat attttaaaat agtctcaaaa
     7081 tcacatatat ttaagttcat agccttatca cgaggccttt ttttaagctc atagacgtgg
     7141 ttcacagctt cttcactcaa agctttggca aagattccgt agaccgtttc tgtcggcata
     7201 ataactgcgc catgatcctc taaaatctgt cccaaatcat tcattatcta ataccaccat
     7261 tctatcttta ccaaatatat ctttcaaaac tctgcaacga ttgtttggaa aatatctttt
     7321 caataaactt cgtaaatcat caccttgttt gtaacctatt tcaaaatata atttacctct
     7381 aggttgcaaa tattctcttg agttttcaat gattttacga taaatagcta atccttcttc
     7441 gtctgcaaaa agggcactat gaggctctga agctagcaca ttcttcccca cctcgtcttt
     7501 gtcattgtaa gaaatatagg gaggattaga cacaataata tcaaatttat ctgaaatgtt
     7561 actaaagaca tctgattcta taaaagtcac ttccgcatca caattattag cattttcctt
     7621 tgccaagtct aatgcagata cagaaatgtc actagctaga acatcccaag aggggcgttc
     7681 tttttttaga gaaatggcaa ttgctccact acctgtacca atatctaaaa cagaacaatc
     7741 ttctacttta ttctctgata agattagatc aaccaactcc tctgtctcag gcctcggaat
     7801 taaaactcgt tcatcaacaa agaaaataag gtctctaaag taagctttac ctgtaatata
     7861 ctgcggtgat ctatgttttt taagctgttg aaaaatattt tccattaatt gctgatcagt
     7921 atcactcaca ctactatttt gatttaaaag gtaatcagtt gttgaccatt gttttaggtc
     7981 ttttaataca taaataaaat tttcgacttc ttcaccacaa gcctctaaca actgttcata
     8041 atgcttgatt aattgcgcat aattcattat ttatttaagg cttctaactt ttgagtctga
     8101 tcatacataa caagtgcatc aataacttca tctatttttc cagataaaat agtatctaat
     8161 ttttgaagtg tcaaaccaat acgatgatct gttacacgat tttgtgggaa attataagtg
     8221 cgaatacgtt ctgaacgatc accagtacca actgttgatt tacgttcagc atcttgttca
     8281 tcttgagcaa tttgcgcaaa atggtcagca acacgagcac gaatgatttt catcgcttta
     8341 tcacggtttt tttgctgagt acgctcttct tgcatttcaa ctttgattcc tgtaggaatg
     8401 tgtaccatac gaactgcagt agcaacttta ttgacgttct gaccaccagc accagacgcg
     8461 tggtaaatat cgacgcgaag gtctttttga tcaatttcgt attctacttc ttcaacttct
     8521 ggcattacca aaacagttgc agttgaagta tgaacacgac cctgactctc tgtcaccggg
     8581 acacgttgaa cacgatgagc tcctgattca tatttaagct tagaataaac tgactgacca
     8641 gaaaccatag caaccacttc tttaatacca ccaacaccat taacggatga ttccataact
     8701 tcaaaacgcc atccttgagt ttcagcatat ttttgataca ttgttaaaag atcaccagca
     8761 aaaagcgctg cttcatcgcc accagcagca ccacgaattt ctaaaataat gtttttatca
     8821 tcattaggat ctttaggtaa tagaaggatt tttaattttt cttcatactc ttctttagct
     8881 gcttttgatt ctttaagttc ttctttagcc atttcttcca attcagcatc accagaagca
     8941 tctttgatca tttcttcagc atctgaaata ttttgaatga cttgcttata ttctctataa
     9001 gctgtgacag tttctcgagt attagcttct tcacgtgata attccataaa tcgctttgta
     9061 tctgaaacaa catctggatc actaagtaac tcacctaact cttcataacg atcttcaact
     9121 gcctgcaatt gatcataaat attcattttt ctttgtatct cctttaaaac cgattatctt
     9181 ttaaatatct ggattaaaat aatgttttcg acaaactggg atatatgttt cattaccacc
     9241 aatttgtatc tgatcacctt catagacagg ttttccgttt tctgtcctta aaaccatggt
     9301 tgctttctta ctacagtatt ggcaaattgt tttgatttca tcaatcttat cggccaataa
     9361 tagcaaatgt tttgaacctt caaaaagatt gttttggaaa tcatttttca aaccaaaagc
     9421 catgactggt acatctaagt catctactac tctagctaaa tcgtagacgt ttttcttact
     9481 caaaaattga cattcatcaa ttaaaacaca ataaggcttt tgtggtaaat tctgaatata
     9541 ggagaaaatg tccatatcat ctgaaattgg aactgcttca cgacgcattc caatacgact
     9601 tgaaacgact ccaaattcat ctcgagtgtc caaggcgctt gtcataataa ccacaggttt
     9661 tccttgttct tcatagttat gagcaacttt taaaatttca atggttttac cagaattcat
     9721 tgtcccatat ttataataga gttgagccaa agcggaaaat cctactttct atagtattct
     9781 acctatttta ccacaaaggc tgaagttttc ccagccattg aaaacagtgt gtactttttc
     9841 acaacattat gttacaatga tgcccaataa cataaaggag taacattatg ccatttgtaa
     9901 aaattgactt gtttgaggga cgctcacaag aacaaaaaaa tgaattagct cgcgaggtta
     9961 ctgaagtagt atcacgcatt gcaaaagcac ctaaagaaaa tatccatgtt tttatcaacg
    10021 atatgcctga aggaacctac tatcctcaag gtgaacttaa gaaaaaataa gaaatagtaa
    10081 aagaggttac ccaggtaacc tcttttgata gtctttgcaa gcatatttct tgacaaattg
    10141 aaaatatttg aaaataagta ttgattttca agttagaaaa cggtatcatt agaatatatt
    10201 gctttggggg tggaaaattt gcaactttct catagtttaa gactcatggg aacgactatc
    10261 gatattcaaa ttaattctaa aaatgctcaa aaacaaatcc aagaggtcat agaagtcttg
    10321 gagctctaca aaaatcgctt tagtgcaaat gactttaatt cagagttaat ggctataaat
    10381 aataacgctg gtatcaagcc tattcaagta catccagacc tctttgaatt aatcacaatt
    10441 gggaaagaac acagcttagc taggcctagt aacctaaata ttgctattgg tcctttagtg
    10501 caaacttgga gaattggatt ttcagatgct aaactaccta gccctagtga aatatcagaa
    10561 gctatgattt tatctaaccc aactcatata cttctagatt caaaaaatca atctgttttt
    10621 ttaaatcaga taggaatgaa aattgatttg ggtgcacttg caaaaggata tatcgctgac
    10681 aaaattatga cctatctcaa aaatgagatg attgattctg ctatcattaa cttaggcgga
    10741 aacgttttag ttcacggaga taatcctaat cgttcagaag ggtattgggt tatcggcatt
    10801 caacatccga aaaagaaacg gggacaaaat attggagctg tcaaaattaa aaaccaatct
    10861 gttgtaacct caggtactta tgaacgccgt ctgattattg acgataagga ataccatcat
    10921 atttttgatc gacagactgg ttatccgata caaacagaga tggctagcat ttctattgtt
    10981 tctaaacaat ccgtcgactg tgaaatctgg accacaaggt tgtttggctt atccattaaa
    11041 gaggctcttg atattttaaa cgctgtttcc tacattgaag gcattatcat cactaaagat
    11101 gatcgtatct atttatctga tggattaaaa catcattttc aactatttta tcattaagaa
    11161 agaggatttc taatatgaaa cttattggca tcgttggaac taactcaaat caatcaacaa
    11221 atcgtcaatt attacaatac atgcaacaac actttgctga caaagcaaaa attgaactca
    11281 tcgaagttaa agaccttccg ttatttaata aacctgctga taaaaatgtt ccccaagtaa
    11341 ttttggatat tgctgccaaa attgaagaag ctgatggtgt tattattggt acgccagaat
    11401 atgatcattc tataccttcg gccttaatga gtgttttagc ttggctatca tatggtattt
    11461 acccacttct caacaaaccc gttatgatta ctggtgcttc atatggaaca cttggatcat
    11521 cacgtgcaca attacaatta cgtcaaatac ttaacgctcc tgaactaaaa gcaagtgttt
    11581 tacctgacga attcttgtta tcacactctc tccaagcctt tgataaagat ggcaacctac
    11641 acgatatcga aacaagtcag aagctagatg ctaactttga tgattttcgt ctttttgtta
    11701 agattactgg taaattatca aatactcgtg atttacttca aaaagaagca gaaaattttg
    11761 actgggaaag tttataagag tatagaaggg aataactaag atgaaatttg taggtattgt
    11821 tggctcaaat gcagagcaat catataatcg tatgttgctt gaatttattc gcaagaattt
    11881 taaaaccaaa tttgaattgg aagtccttga aatcgatgat attccaatgt ttaatcaaga
    11941 ccaaaattgg gaagaaagtt tccaacttcg tcttcttaat aataagatca cacgagctga
    12001 tggcgttatt attgccactc ctgaacataa tcatactatt acggccgctc ttaaaagtgt
    12061 gcttgagtgg ctctcatttg ctgtgcatcc actcgaaaat aaacctgtta tgattgtcgg
    12121 tgcttcatat tatgatcaag gcacatcacg tgctcaaata caccttcgta aaatccttga
    12181 tgctcctggc gttaatgctt acacacttcc aggaaatgaa ttcttattag gaaaagctaa
    12241 agaagccttt gacgataatg gaaacattat caatcctggg actgttcaat ttttagaaac
    12301 ttgtcttgat aattttatca aatatgtagg agttgtatca tcattgagaa aacctaaagc
    12361 tattgaatct gaagacctct actgtcaggg acaaattgat acaaccatta ctggtatcga
    12421 cccagatgat cctgattggt tagacaaggc agctgagaaa gttaacgccg ttgaagggga
    12481 tgcttacgtt aaactagaca atggtttact tactgttaac caacttaata tgtttttaaa
    12541 tgctatgcca tttgagctaa cgtatgcaga tgataataat caatttctat attacaataa
    12601 taatcatcca gatccagata caatgttagg aaaacgtgtt cgtgaacaag taggaaaccg
    12661 tatggctaca gttcatggta gtttaccacc ggcacgaatg aaaaatgttg aatgggttat
    12721 cggtactctt cgtaatggaa atcaagaata tgtacgtaca gtcgttcctg gtacacctcc
    12781 agaagttatc aatactcaca actatcaagc tatgtactac ccagacggat cttatgctgg
    12841 tattaatgaa attatcttta actttaaacc gtggttagat tggtacctcc aaactactgg
    12901 acaacgttta gtcggtggaa ctactgctgc tcctggagct tcagcagttg acgctacttc
    12961 tagcgcttca actagcacag gggatgctcc cgttaatcaa gccacagatg ccacttctgg
    13021 tgcctctgaa cattaatctc ctcgcttttg aggatctgat accttaaaaa gatatcaaga
    13081 tcctcctttt taatatttaa tcaagttaat aaaatcacat aaaaggaata tactatgtca
    13141 ctttttcaag aaaaaattgc ttacaattgc gctaaaaagg aagcgcttta taaagagagt
    13201 ttaggacgct acgccttgag gtcaatgcta gcaggggctt atttaacaat gagtactgct
    13261 gctggtatcg tcgcagctga tactattggt aaaatttctc ctgctctatc aggttttgta
    13321 tttgctttca tctttagttt tggacttatt tatgttttaa tatttaatgg tgaattggcg
    13381 acatctaata tgctttatct cactgcagga gcctataata aaaatatctc ttggaaaaaa
    13441 gccatgacaa ttttaattta ttgtactttt ttcaacctcg ttggtgcttg catattagct
    13501 tggttgttta atcaatcgta ctcattccag catttaacaa atgatagctt tttagggcat
    13561 gtcgttgcta aaaagttagg aaaacctagt tcaggagctt tcctagaagg tattattgct
    13621 aatatgtttg tgaatttagc tatccttgct tacatgttat taaaagaaga atcggcaaaa
    13681 atgacggtta ttttatctgc tatttttatg tttgtttttc tatcaaacga acacttaatt
    13741 gctaattttg catcctttat gttagctgct tttagccata tcgaacatat taaaggtttt
    13801 actcttctaa acgttatccg ccaatggaca cttgtattct ttggtaactg gataggaggg
    13861 ggcgttttca tcggcctagc ctatgcttgg ttaaataaaa caaaaacttc tcaccatgaa
    13921 taaaagaaaa actgagtaat ttattactca gtttttcttt tatattctca tcatgaaatc
    13981 aataaaaatt aacttttttg ataaataaag gaaagtatca tattaatttt ttaggcaatc
    14041 cattcaaaat gatatttaaa acaacagctg tcagggttga gataacaata ccatttgtca
    14101 aaaacatttg taaagtatta ggaagactaa tgaagaggtt tgtcccatta aaacctactc
    14161 ctgctgcaat tgatacggct gcaatgataa agttatgctc attatgctca aaatcaacct
    14221 gattaagcat tttcatccct tgaagtgcta ccataccaaa taaaactaac atagcaccac
    14281 caagaactgg acttggaatc atttgagcca ttgcgccaaa tttaggtaac aagccaagta
    14341 taactaagaa gagagctgta aaatatatcg gcttacgcgt acgtatccca gatatctgta
    14401 ccaatcctac attttgagaa aaaccggtat atggaaaggt attaaataag ccgccaagta
    14461 atactgccaa tccttctgaa cggtaaccat tacgaagtct cttactgtct aatttatcgt
    14521 ttgtaatatc tgaaagcgca aggtaaacac ctgttgattc taccatagaa actgttgcaa
    14581 taatacacat cattaaaata gaagtaaatt caaatctagg agctccaaaa taaaatggct
    14641 tcggaatatg tacaagtggt gcatctgcta ccacagaagc atcaactaag cccataaatg
    14701 ctgcaagaat tgtacctgat atgagtccaa taaggattga aattgacttc aaaaagcctt
    14761 ttgcgaaaat attaattaat aaaacaacac caatcgttac cagtgataaa gttaaagatt
    14821 gtaagctcgg ttcttttgca ttatcaccca tatttcccat cgcaactggt attaatgtca
    14881 aaccaattgt agtaataact gatcctgtta caattggagg aaagaaattt gcaactttag
    14941 aaaaaacgcc agcaactaaa acgacatata ttcctgaagc aattaaagct ccaaacatat
    15001 agccagaacc ttgttgtgca ccaataattg ataagggagc tacagactga aaggcacacc
    15061 ctaatacaac tggaagaccc actccaaagt gcttacttaa tcgtaattgt aataaggtgg
    15121 caatcccaca cataaaaata tctgtcgcaa taagataagt tagttgctta gcattataac
    15181 caagagcact agcaatcatg ataggtacaa gaatggatcc tgcatacata gctaacaggt
    15241 gttgcaagcc tagaagtgct gcctgcgagt tactttgctc attaaatttc attaagcatc
    15301 agcctccgca aaaacaactc gaccattttc aaacgcttta atacgagcaa gagaagtaac
    15361 tggaacacct gttttttcta acaaatcacg cccatcttgg aaagattttt caataacgat
    15421 tccgatacca gcaaccttag ctccagcttg accaataatt tcaagtaatc ctttagccgc
    15481 ttgaccgttt gctaaaaagt catcaatgat gagtacagta tcatcgttag ataaaaagcg
    15541 actcacaata gaaacttgac tcgtaacttg ctttgtaaaa gaatacactt cagcagttaa
    15601 gataccttca gtcatagtaa tgttcttagc ttttttagca aatatcattg gtacgcccaa
    15661 tgcttgagct gcgtacactg ctggcgcaat tccagatgct tcaattgtaa caaccttcgt
    15721 aatgccggct tctttatatt tatcagcaaa aactttacct atttcctgca ttaactcaaa
    15781 atctacctga tgagtcaaaa aagaatcaac ttttaaaatg ttctcaccta aaacatcacc
    15841 atctttaaga atacgttctt ccaataactt cataataacc ccttcactct ttagaaaata
    15901 aaaaaataac ttacctcaat cagaggacac aaatctcctc taatttatcg cttcattaac
    15961 atgaaccaac aggcaagcta tttattagct tacttattgt tagacattac ggtgtcttgt
    16021 agagacgcta agccaatcct cagcataaat aagtaaatca tatatttaat tgttatgtat
    16081 gatagataac tattgtaaca tttttaatta agatttcaag atttaattta aatggaatca
    16141 ccattccaaa tagaattttt aacaataacg taatcaacgt gtctcaagct atccaattct
    16201 tttcctcctg cgtaagaaat agaactttgc aaatcttgtt gcatttctgt caaagtatcc
    16261 tctagtcgcc ctttaacagg taatagaatt ttcttacctt caacattttt atgttctccc
    16321 ttttgatatt cagaagctga gccatagtat tctttaaatt gctgtccatc tacttccaca
    16381 agttttccag gactttcaag gtgaccagca aacagcgaac caatcattac catactagca
    16441 ccaaagcgga tagattttgc aatatcccca tgtgttcgaa taccaccatc agcaataatt
    16501 ggcttgcgag cagctttaga acaccaacgt aaggcagcta attgccaacc tccagtacca
    16561 aaacctgtct tgactttagt aatacaaact ttaccaggtc caataccaac tttagttgca
    16621 tctgcacctg cattttccaa ttctctaaca gcttcaggcg ttcctacatt tccagcaata
    16681 acgaaagttt ctggaagctc ttgtttaatg tgttggatca tctctataac actgttagaa
    16741 tggccatgtg caatatcaat agtgataaac tcaggagcat cttctttcag acttgtcacg
    16801 aagtcatatt catagtcttt gaccccaacc gaaatcgaag caattaagcc tttatcatgc
    16861 atacgcttga taaaaggtct tcttgcttct tcattaaaac ggtgcataat ataaaaataa
    16921 ccttcacacg ctaatgtttc agctacctct tcatcaataa tagtctgcat attagcaggg
    16981 attactggaa gtttaaaggt atagtttcca agtttgacac ttgtgtcagc ttgtgagcgg
    17041 ctagatataa tacacttgtt tggtattaat tgaatatctt cataatcaaa aacaggaata
    17101 tcgttaaaca taatggttct cctactgagt aaaatgacct tcctataact ttgtataaca
    17161 aagcatcgct aggtaaaaac atacctttcc tttttaaatt atagacttta tttatttttt
    17221 agtcaatttt tgctcaattt atttgaataa tgttcgtttt tgcaaaagaa attaaaatcc
    17281 tatagagcag tactctatag gattttacat ttattatatt gcttttcttt ttcgaagata
    17341 agttaatcct acaatagcta agataacaac aacgataacg aatagaactg caaattgact
    17401 accaattata gctccatttt tcggaatagc tacttgtccg atggtaaaaa tttgtgaggc
    17461 tactgcggtt gcaaaagctc ccgcaaattg ttgtagtgta ttcaagatac tattaccatc
    17521 cgctgactgt gctggcttta gttttgttaa agctgtcgta accatattac ttgctccaat
    17581 ccccatacca attttagtaa gaatatcaaa ggctaaaagt actacaacag acttcgcagg
    17641 aatgaatata gctgtaccaa ttaaacctat caaagaaagg gagataccta tcataattgg
    17701 cttaaatgca ccaattttat ccaggagata acctgcaaaa ggggataaaa aaactactgc
    17761 tattgcacct ggaaacataa acaaaccagc tttggttgaa gtttgatgta aaacaagttg
    17821 caagaggtta ggtaataaga acgaaagtgc tagaggaatc atttgaaaag ccaggacgcc
    17881 atacaataag attgagaaat ctttatcttt aaaaacattc aaattcaaaa attctagctg
    17941 tttatttaaa tagtaaaaaa taacaaaact aacaatagct actaataagt aaaaaataga
    18001 catcttttca atagctaaaa gaagggttgc caagccaata cctaaactga caaaggcagg
    18061 aaaattgagt ttatcattaa ttcttacttg acgtttggga atagaagcca aacctataaa
    18121 ggtggatatg ataagtattg gtgccagaag cataaaaatc atcttccatc ctaacattcc
    18181 tgaaataaca ccgccataag taggtccaac tgccggtgct aaaagtgtga ccatagcagc
    18241 tactcccatc attagtcctc tacgctccat cgggatttgt gtaagaatga tatgaaacaa
    18301 taaagggagg gctaatcctg tcccaacacc ttgcaagact ctccctacta acaatatagg
    18361 taaattaggg gcaaagctat caattaaaac accagataaa aaaagcacat ttgccaatgt
    18421 aaaaatttgt ctctctgata aatttttaat cataaatgct gacaaaggca cagtcacagc
    18481 aacagctaac aaatttcctg ttgtcaccca ttgaataaca gcggggttaa ccccaaattc
    18541 tttcatcaaa agcggaaaag tgacattcat agatgtttca ataagaatcc ctgaaaaacc
    18601 taatatagcc gttgtaatta tggcaggcat aatacgccat ccctttttag tcattaaaat
    18661 ctccttgctt tgattattat cataaaatag agcaccccgc taaatcaagg tgctccattt
    18721 aattcattcg aattaaaatt aaattgttta tattagttgt caaaggacaa tcaacagctc
    18781 ttattatatc aaataaagct aagaaaaaca aagagttttt tgaatattaa tgtcttcact
    18841 ttcttatcta ttctctaccc atcaatcacc acttcagaga acataacaat tgcaatttac
    18901 tcttaatacc gttatcatgt tcatatgcta atacccaaga aaatagtggc ttttgcctca
    18961 aatgaatctg aaaataattt ctttattacg ttaaagactt actttaatta tttattttct
    19021 atacaaatca ttacagatat ttcaactctc aatcatgcag attttgacgg tagctttgca
    19081 ttccatgata tagaaactag catacctcat ctggtaattg actccaacta cttagcgatt
    19141 agtcagacta actcaaaaat agaggctaac gatattaaaa cgttctcgga actctccaaa
    19201 acaatgaccg agtttcacta tatgctaaat tttgacttat ttaaccatct cccatatcgt
    19261 tttagattgc ataataagga cggtcaaaca atttattcga accataaacc tgaagatcct
    19321 tttgatattt atcctgaaga agaatatcct atagacaaat gggttcaaaa cagcctaata
    19381 gagaaaaaag cgaaagagct acatttactc ttacctagcg catcacaaga ttatattcta
    19441 gtacaatctt ataaacgctt agaaaatgat agtgggcaac tagttggtta tattgaacat
    19501 gttcataata ttaaaccact gctagagggt tatttaaaag aatccggtca agctatcgtt
    19561 ggatggtctg atgtcacgtc tggtgcttct atctctaacg atgattttga actataatcc
    19621 actcgtcact ctaaaaagaa aactaataac taaaaggcac tggtttaatt tcagcgcctt
    19681 ttattaatca atctccagtt gttgtaagtt caattttttc tttaattggt agacctcgat
    19741 tagctgtaaa aatcaaagga actacttcgg ttgtaacttc ggaaaattgt agaccaaacc
    19801 atcgtattgg tgttgctgtc caatgcttta tagatgattt cattagcata acaaatcgtt
    19861 caccaggttt taggttttga ctacttgaaa aacgttcatc aaggacaata aacctaaaat
    19921 ctcctacttt actatcaggt ctaacgctgt atgtctttcc ttgtttaggc agacgtccac
    19981 tttcaagtaa ttcttctaca atcgtacgaa gatagcgtga cacagtctga cgcatcttaa
    20041 atccaaggta aagttctacc ttcacaatga agtcggttcc catcatgtca accttatact
    20101 cagctgtata aggttcatca gtgaccttaa tgtttacaaa ccaataaact tgtgcttttt
    20161 tagggcgctt atcaaggata gaatacatga tagacttatc aatcatgtct ccttccatac
    20221 gatttgtcaa ataaacaacg ttagtatgat aaatcggata acgatgatca tgtctaagtt
    20281 tatctaattg tccgatgtaa tcttttaaat ccaacaactt aacataacgc gaaacaattt
    20341 tactaccttt ataccagata gtcatgatga atataattgc tactgctata ataacaacaa
    20401 catagcctcc atgcataaat ttaacagctg aagcaaggaa gaagattccc tctaaaatac
    20461 caaagaaaat catcatcaat aagactaaac ctctttttac tcctttttga attaaaaaga
    20521 aagacagtaa aatagttgtc attagcatcg taattgttat cgctaatcca tatgccgctt
    20581 ccatgtgcgc tgaagtctta aaaagcaaaa caatagaggt tgtaatggca aataagaacc
    20641 aattaataac aggtatgtag gtttgaccaa tattgtcacc aggataagtt gaacgaaatt
    20701 gtgggaatat ttttagtcgc atagcttcag aaactaaggt aaatgatcca gaaatcagtg
    20761 cctgtgaagc gatgatagct gccaaagtag ctaaaataac gacatgcatt gtaaattgcg
    20821 aagggatact agcaaaaaag ggattcaatt catttcctgc gttcttatta gctaaaatcc
    20881 atgccccttg cccacaataa gaaagtataa tggcaacctt aacgaacggc catgaaacat
    20941 gtatatttcc acgcccaaca tgacctaagt cagagtatag tgcttctgcc cccgttgtcg
    21001 ccaggaaaat agatcctaaa ataaaaatac ctttatgatt ctctagacta aataacaatt
    21061 tcaaaccgta gtatggatta attgctttga aaacttctgg atgggcaaaa ctattaagga
    21121 aaccgctaat acctaaaaag gcaaaccata taaacataat tggcccaaat aatttcccaa
    21181 tcacacccgt tccaaacctt tggatggcaa aaagtaacag taaaataaac aaggtcgcaa
    21241 aaataacgtt actctgattc tggaaaatat gctgcaaact aggaactact tttaatccct
    21301 caacggctga agttacggtt acagctggcg tcaaagctcc atctgacaac aaggttgcac
    21361 ccccaataac agctggcaca attaaccaag gtgtcatttt tctaacaagg gtatataaag
    21421 aaaaaatacc accttcgtgg tgattatccg cctttaaagc tacaagcaca tacttgatag
    21481 ttgtaataag tgtcagggtc catatgatta aagatataga acctaagata aacgattctg
    21541 tgacactaga aataccacct tggttttcaa ccaatgattg cattgtatag agtgggcttg
    21601 taccaatatc tccataaaca atgcctaaag caataataaa tcctgctttt gatgctttat
    21661 caaaagaaga atgattgaca tgttgcattt ctgccaaccc tccgattaaa taatattagt
    21721 caaaaaaatg gctgaactca ttatagcaga ttaagttaaa aaaacaacac ttatctataa
    21781 aaagaccaga aattattaaa gcgtttcctg gtctttacta ttttaatcac gataaacagg
    21841 ttgaggtcca taagtttgac tgactggcat tatttctatg cgattaatat taatgtgttc
    21901 tggttggcta gcaacccatg acacggtatt agcaatatcc tctgcttgaa ttgcatgcgc
    21961 accttcataa agtttttcaa ctcttttatg atcccctttg aaacgtacag ttgaaaattc
    22021 tgtcccttca cataaaccgg gttctaggtt agtcactctg attttagttc cagctaaatc
    22081 ggcacgcaag ttgagagaaa attgttttac aaaagctttt gatgcgccat agacattagc
    22141 tcctggataa ggaattgttc ctgcagttga tcccaaattg ataattaacc ctctattaac
    22201 ttctaccatt ttaggtaaaa tgcatcgtgt caagtaaatt aatcctacaa cgttagtatt
    22261 aatcatagtc atccaatctc caaaatcagc ttcatatgat ttatccaatc caagtgctaa
    22321 acctgcatta ttaaccaaca aatcaattga ttgcaaattt ttaggcagac tttctaaagc
    22381 tttgtcaacc gattctctac ttgtaatatc catttgaagt ggataaaagt gaccctctga
    22441 aaacttttct ccaaggcatt ttaatttatc cattcgacga gcacaaccaa taacgctata
    22501 accatctgaa actaaattag taacaatagc tgcaccaaaa ccagcagatg cccccgtcac
    22561 aagcgcaact ttacgtgata tcatagacac tcctcctcta gtatatagaa aaacgagcta
    22621 ggaaaaatcc ttctcgtttt ataaattatt ttgatttctc atcaatagct tgagcagctg
    22681 taatgatagc aagtttataa atatcttcag cacttgatcc acgtgataaa tcatttactg
    22741 gagcattcaa tccttgtaaa atcggtccga ttgcttcaaa catacccaaa cgttgtgcaa
    22801 ttttatagcc aatatttcct gattgaaggt ctgggaaaat aaaaacattt gcttttccag
    22861 caacatcact attaggagct ttaatttctg cagtttccgg aacaaaagct gcatcaaatt
    22921 gtaattctcc atcaacagct aattcgggag ataaatcttt ggcaatttta gctgcttctt
    22981 gaactttttc agcttgaggc gcttttgcac ttccttttgt tgaaaaacta agcatagcaa
    23041 tcttgggatc aatatcaaat attttagctg tatccgcagt atttactgca atttctgcta
    23101 gctcttgtgc attaggatca atgttaatag cacagtcagc aaaaatataa cgttcctgtg
    23161 tattttcacg gttcatcaag aatacacctg aagtgcgtga aattcctggt tttgttttaa
    23221 taatttgaag agcaggacgt acagtatcag ctgttgaatg aatggcccct gataccatac
    23281 catctgccaa accaagtttt actaacataa ccccaaaata gttaacatcc ttcaaaagac
    23341 gatctgcatc ttccatagta gctttgcctt tacgaatctc aacaaaagct tctttcatct
    23401 cttcaaattt ttcgtattgt tcagggtcaa taacgctata atcttgatca acaaaaccaa
    23461 gtcttgccaa agaatcgtga acttcgtcag ccttacctaa aataacagga tcaacaagac
    23521 cttcaaattt caaacgagct gctgcacgta atacacgttc atcgcttccc tcagggaaaa
    23581 caatcttaac ataatcactt gcaacttttt ctcttaaacc accaaataag gaacgcatat
    23641 atataaaacc ttctatttct aaattaatgt aattccatga taacgctgtc aaagtcatct
    23701 gtcaagtcta gcgtctgttt atttattttt cccataaaag ggtcgtaaaa agacaaagaa
    23761 tgacaatgca atgcttgcct gtttattcct agatccatac gtcctccgta caaatcatct
    23821 cccaaaagag gaaagccgat atgagcaaag tgaactctaa tttgatgagt acgccctgta
    23881 tgtaatttga tatccactaa tctaacatca ccataacgag caactacttg ataactagta
    23941 tgtgcatatt tgcctgattc atggactcgc ctcgtaataa tactatcaac atctcgtgca
    24001 ataggagcta taatatcacc actatctgct aaatcaccac tccctgatac taatgcataa
    24061 tacctttttt caattgcttt agcctgaagt tgtttatcta gacgagcatg ggcataacca
    24121 tgcttggcga ataacattaa tccacttgta tctcgatcca accgggtcac aatatggacc
    24181 tgctgattgg catagttatt agacacataa tagtgtttta taaaattagc aattgtattt
    24241 gaatgaatag agctaggaat actagcgaaa ccaaaaggtt tatttaaaat caaaaaatga
    24301 tcatcctcat aaacaatatc taaatcgtga ggtactggtt ccaaagtttc gtgagaaggc
    24361 tcgtcaggaa tatctatcgt aacaacatcg ccaatctcta ataagaaaat agcgttctgc
    24421 tcttcaccat tgacaaaaat cttaccacct ctgtacttaa tctttgctaa taaccccctt
    24481 gaaacatcat gacttttcaa aagtgtttta actttgcacc tttcttctgc tatgtattca
    24541 aatttcaatg ttcaggttct ccgataaaag catcgttaac acgttcccaa aagctagtgt
    24601 gacttggagt tgaaacaaag ttaatacttt tttcgtcaat actatattct attttggtaa
    24661 catttttgta atggaccgtt ttgttatcta tagaaattgt atagacacca actcgtttag
    24721 gaactatctc tatagcatct tttttgggga tgataaccga tgaccccaag gtacgataga
    24781 cgcgattatt aagactagaa atttctgtta gttgcaaggc ttctatcgtt gggtggagta
    24841 ctgccccccc tagtgattta ttgtaagcag tactaccggt tggtgtagaa actaaaatgc
    24901 catctcccct aaacctttca aataccactt gattaataac aacatccgct accattgttt
    24961 tttcaatacg cttaatagta gactcattta aagcacgcgc acgaataact cgaccatctt
    25021 caagggtaat agttactttc aaaatgggat aggagatttg ttcaccttca tcatttttca
    25081 aattattaat taatgtgtca acttcaaaat ctcgatagtc tgtataaaac cctaaatgtc
    25141 ctgtatgaac accgacaaac cttactttat ccagttgttt ttcgtacata tggaaggctg
    25201 acaaaagcat accatctcca ccaatagaaa tgacaatatc aggatccttt tttgataaat
    25261 aaaaatcagg atcatgttta aaagcagcaa agagctttga ggctacacgc ttgctttgat
    25321 attttccgtt agcaattata gcaacacgtg ttgctctatc tgtaaaattc atctgtgtca
    25381 tcactatttc ctacaccatc gctcaatttt cgatttaaag gatcaaataa aagctgtgct
    25441 tcacgaatat cttcgcgaat cttacgcatt tcttcatcca attctaaagc gatttttgct
    25501 gttttctcca gtctttgttt aatttcttca gggaagtccc cctgatactt gtaatttaat
    25561 gaatgttcga tggttgccca aaaattcata gccagagttc taatttgtat ttctgctaaa
    25621 actttctttt gtccatctat tgtgtctaca ggatactcta caacgacatg atatgaacga
    25681 tatccactag atttcatatg ggtaatatag tctcgctcct gaaccactgt catgtcatga
    25741 cgttttctca ataaggcaag gacttcatca acgtcatcta caaattgtac catgatacga
    25801 agacctgcaa tatcttgcaa gtcctgagca agattttctt ctgaaatacc acgtaaaacc
    25861 atcttttctt gaatactttc aaccgacttt acgcgtccag taacaaattc tattggagag
    25921 tgacgatttt gctttctaaa ttgtttacga ataccacgaa gctttatttt taattcacca
    25981 accgtttgaa tataagggtc tagaaatgtt tcccaatcca ttgacattct aatcctccaa
    26041 aatatttaat gtactctctt gtaaaataaa ctttttttct ataaaattaa cacaacaatt
    26101 aatattatac catatttagg aaaaggaagt gataagatga cacacctaga aattgaatac
    26161 aaaactctat tgaataaaga cgaatttaat cgtctaacaa gcttattttc tcatgttcaa
    26221 cctattactc aaaccaatta ctattttgac acagaaacat ttgaaatgaa agcacaccga
    26281 atgtcacttc gcattagaac gttacctaac cgtgccgaac taacactaaa aatccctaga
    26341 gaagtaggaa atttagagca caatcatgac cttactttag aagaagctaa atatatcgtt
    26401 aaaaatggcc agttccctga agatactgag attgcttctt taatccttga aaagggagtt
    26461 gatccaacca aattagctgt ctttggacaa cttacaacaa caagacgaga gatggaaact
    26521 tcaatcggct taatggctct agacagcaat atatatgctg atattaaaga ttatgaactt
    26581 gagttagaag tcaaacaacc taaacaaggg aaaagagatt tcgaccagtt tttaaaagaa
    26641 aataatatta attttaaata cgccaaaagt aaggtggcta gattctctgc tactttaaaa
    26701 aataactaac gttgagagat tggattttcc aatctctttt ataattctca tttaagcaag
    26761 aacttaaaat ctcatactat ctctcctcaa cattgttatt gcaagcattt tcatgtttct
    26821 tatcgtgaat tttttctatt tttttggtaa actattagta tcaatctaaa ggagtcatct
    26881 atctcatggc agaacaatac gccgataaac aaatcaaact tttctcatta accgctaata
    26941 gagaaattgc tgaaaaaatt tcccaagctt caggaattcc tttgggtaag atgtcatcac
    27001 gtcaattctc tgatggcgaa attatgatta atattgagga aacggtgcgc ggtgacgata
    27061 tctatatcat ccaatctaca agcttcccag tcaatgataa tctctgggaa ttactcatta
    27121 tgattgacgc ttgcaaacgt gcaagtgcta atactgttaa catcgtagtc ccttattttg
    27181 gatactctcg tcaggaccgc atcgctgcat ctcgtgaacc tattactgca aaattggtcg
    27241 ctaatatgtt agttaaagct ggagttgatc gagttcttac cctcgactta catgcagtgc
    27301 aagtacaagg gttcttcgat atcccagttg acaatctttt tactgtccca ctatttgctg
    27361 aacactataa tcaactcggt ctctctggtg aagatgtcgt cgttgttagt cctaaaaatt
    27421 ctggaatcaa gcgtgctcgt agtttggcag aataccttga ctctccaatt gctatcattg
    27481 attatgcaca agatgattct gaacgtgaag aaggctatat tattggtgag gtagaaggta
    27541 agaaagctat tattattgat gatattttaa atacaggaaa aacctttgcc gaagctgcaa
    27601 aaattttaga acgtggtgga gcaactgaga tttatgctgt tgctagtcat gggctttttg
    27661 ctggcggcgc agctgatatt ttagagtctg caccaattag agaaattatc gtaacagact
    27721 ctgttttatc taaagagcgt attcctagca atattaaata tctgaccgct agccatctca
    27781 ttgcagacgc tattatccgt atccatgaaa gaaaaccttt aagtccatta ttttcatatc
    27841 gttcagataa gaaggattaa ttgatgattt atttagacaa tgctgctact accgctctaa
    27901 ccccatctgt tattgagaaa atgaccaatg tcatgacaag taactatggt aatccatcta
    27961 gtatacatac ctttgggcgt caagcaaatc aacttttacg tgaatgtcga caaattattg
    28021 ctgaatatct aaatgttaat tcacgtgaaa ttattttcac ttctggggga actgagagca
    28081 acaatacagc tatcaaaggg tatgctcttg caaatcagct aaaaggtaaa catattatta
    28141 cctctgaaat tgaacatcat tcagtcctac atactatgac ttacttatca gagcgatttg
    28201 gttttgatat tacttactta aaaccaaacc atggacaaat tactgcaaaa gacgttcaag
    28261 aagctttacg agatgatact attatggtat ctctcatgtt tgctaataat gaaaccgggg
    28321 actttttacc aattcaagag attggtcagc ttctcaggaa ccaccaagct gtttttcacg
    28381 ttgatgccgt tcaagtcttt agcaaaatgg aacttgatcc tcattcttta ggaattgact
    28441 ttttagctgc ttctgcccat aaatttcacg gtccaaaagg tgttgggata ctttactgtg
    28501 ctccccatca ctttgatagt ctacttcatg gtggagacca agaggaaaaa aggcgtgctt
    28561 caactgaaaa tataattggt attgctggaa tgtctcaagc tcttactgat gctacgacta
    28621 acacccttaa aaattggact cacattagtc agctgagaac gaccttttta gatgctattt
    28681 cagaccttga cttctatctt aataacggtc aagactgctt acctcatgta cttaatatag
    28741 gttttcctag acagaataat ggcttgttat tgacacagtt agatttagct ggattcgcag
    28801 tttcaacagg ttctgcatgt actgcaggaa cagtcgaacc tagtcatgtc ttaacaagct
    28861 tgtatggagc caactcacca cgtctaaatg aatcaatacg tattagtttt tcagaactaa
    28921 atacccaaga agaaattctt gaattagcta aaaccttaag aaaaattata ggagattaat
    28981 atggcatttg aaaaagaaat aaatcttcca gactgcaatt acatttacac tatcagcccg
    29041 aacattaaaa aatatacctt acgcgatact acattcactc aaaatcgctt aggtaattat
    29101 gaattagagc gtctcctaga aacagtacct aattctggcg atggctttcc tctaaagatt
    29161 acaatcaata aagaactttc tggttttaag ctcttaatta ctgataaatc tggccttaga
    29221 atagtaaata tctttaaatc taccgatcat aaaattcttc aagacaaatt ttatttctta
    29281 atggacagcc tcgtt
//
LOCUS       DATRSD010000013        29072 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRSD010000013 DATRSD010000000
VERSION     DATRSD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29072
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.013"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(7..666)
                     /locus_tag="VBD85_000480"
     CDS             complement(7..666)
                     /locus_tag="VBD85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7133054.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQESYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
     gene            complement(685..1395)
                     /locus_tag="VBD85_000481"
     CDS             complement(685..1395)
                     /locus_tag="VBD85_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133055.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEPSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(1395..2159)
                     /locus_tag="VBD85_000482"
     CDS             complement(1395..2159)
                     /locus_tag="VBD85_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133056.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISIDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(2160..3113)
                     /locus_tag="VBD85_000483"
     CDS             complement(2160..3113)
                     /locus_tag="VBD85_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7133057.1"
                     /translation="MKENMKSILSWLAIVVLDYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            complement(3116..3985)
                     /locus_tag="VBD85_000484"
     CDS             complement(3116..3985)
                     /locus_tag="VBD85_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7133058.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(4091..5257)
                     /locus_tag="VBD85_000485"
     CDS             complement(4091..5257)
                     /locus_tag="VBD85_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7133059.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            complement(5412..5657)
                     /locus_tag="VBD85_000486"
     CDS             complement(5412..5657)
                     /locus_tag="VBD85_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO7133060.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(5757..6347)
                     /locus_tag="VBD85_000487"
     CDS             complement(5757..6347)
                     /locus_tag="VBD85_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO7133061.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(6472..7101)
                     /gene="upp"
                     /locus_tag="VBD85_000488"
     CDS             complement(6472..7101)
                     /gene="upp"
                     /locus_tag="VBD85_000488"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO7133062.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(7211..8380)
                     /locus_tag="VBD85_000489"
     CDS             complement(7211..8380)
                     /locus_tag="VBD85_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7133063.1"
                     /translation="MITYDFTSLPERFSSNTIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKEKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(8516..9064)
                     /locus_tag="VBD85_000490"
     CDS             complement(8516..9064)
                     /locus_tag="VBD85_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO7133064.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(9237..10589)
                     /locus_tag="VBD85_000491"
     CDS             complement(9237..10589)
                     /locus_tag="VBD85_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO7133065.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPTLILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            10795..12144
                     /gene="trkA"
                     /locus_tag="VBD85_000492"
     CDS             10795..12144
                     /gene="trkA"
                     /locus_tag="VBD85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO7133066.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTSFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            12149..13588
                     /locus_tag="VBD85_000493"
     CDS             12149..13588
                     /locus_tag="VBD85_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7133067.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(13612..13863)
                     /gene="yidD"
                     /locus_tag="VBD85_000494"
     CDS             complement(13612..13863)
                     /gene="yidD"
                     /locus_tag="VBD85_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO7133068.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(13863..14585)
                     /locus_tag="VBD85_000495"
     CDS             complement(13863..14585)
                     /locus_tag="VBD85_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7133069.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(14575..15159)
                     /locus_tag="VBD85_000496"
     CDS             complement(14575..15159)
                     /locus_tag="VBD85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO7133070.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(15156..15863)
                     /locus_tag="VBD85_000497"
     CDS             complement(15156..15863)
                     /locus_tag="VBD85_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO7133071.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(15863..16603)
                     /gene="xerD"
                     /locus_tag="VBD85_000498"
     CDS             complement(15863..16603)
                     /gene="xerD"
                     /locus_tag="VBD85_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7133072.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(16593..17066)
                     /locus_tag="VBD85_000499"
     CDS             complement(16593..17066)
                     /locus_tag="VBD85_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7133073.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(17063..17584)
                     /locus_tag="VBD85_000500"
     CDS             complement(17063..17584)
                     /locus_tag="VBD85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7133074.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKELSR"
     gene            complement(17566..18540)
                     /locus_tag="VBD85_000501"
     CDS             complement(17566..18540)
                     /locus_tag="VBD85_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO7133075.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(18537..19331)
                     /gene="racE"
                     /locus_tag="VBD85_000502"
     CDS             complement(18537..19331)
                     /gene="racE"
                     /locus_tag="VBD85_000502"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO7133076.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(19506..19745)
                     /locus_tag="VBD85_000503"
     CDS             complement(19506..19745)
                     /locus_tag="VBD85_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO7133077.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(19903..20445)
                     /locus_tag="VBD85_000504"
     CDS             complement(19903..20445)
                     /locus_tag="VBD85_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133078.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(20507..21028)
                     /locus_tag="VBD85_000505"
     CDS             complement(20507..21028)
                     /locus_tag="VBD85_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO7133079.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(21096..21785)
                     /locus_tag="VBD85_000506"
     CDS             complement(21096..21785)
                     /locus_tag="VBD85_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO7133080.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(21808..22311)
                     /locus_tag="VBD85_000507"
     CDS             complement(21808..22311)
                     /locus_tag="VBD85_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7133081.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(22349..23092)
                     /locus_tag="VBD85_000508"
     CDS             complement(22349..23092)
                     /locus_tag="VBD85_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO7133082.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            23124..23402
                     /locus_tag="VBD85_000509"
     CDS             23124..23402
                     /locus_tag="VBD85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO7133083.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            23487..24419
                     /gene="yidC"
                     /locus_tag="VBD85_000510"
     CDS             23487..24419
                     /gene="yidC"
                     /locus_tag="VBD85_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7133084.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLTLTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKINRNAGKQKRRG"
     gene            complement(24619..25284)
                     /locus_tag="VBD85_000511"
     CDS             complement(24619..25284)
                     /locus_tag="VBD85_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7133085.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKSSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(25308..26165)
                     /locus_tag="VBD85_000512"
     CDS             complement(25308..26165)
                     /locus_tag="VBD85_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7133086.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSSKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(26326..27786)
                     /locus_tag="VBD85_000513"
     CDS             complement(26326..27786)
                     /locus_tag="VBD85_000513"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO7133087.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(27889..28371)
                     /gene="greA"
                     /locus_tag="VBD85_000514"
     CDS             complement(27889..28371)
                     /gene="greA"
                     /locus_tag="VBD85_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO7133088.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(28444..>29072)
                     /gene="mltG"
                     /locus_tag="VBD85_000515"
     CDS             complement(28444..>29072)
                     /gene="mltG"
                     /locus_tag="VBD85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7133089.1"
                     /translation="FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLE
                     GLVEDMISTMNTKMAPYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNR
                     LSAGQALQSNIAILYAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSI
                     SAIEATIKPASTDYLYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
BASE COUNT         9349 a         5746 c         4484 g         9493 t
ORIGIN      
        1 atatttttaa gaaagtggtt ttttcatggt cttttctaaa gtttccacaa taacatcatc
       61 aaatactagt tcttgtttta aaacatcctt gtcagcgtat ccttctagtt gtatctcgac
      121 cgctttctta cctgcttttc gttcaataat gactaaacgt ttaacactta aatttgcact
      181 agaaagacga tttaggactt ttgatagtac ccctatcccc tcatcagcta atataaccaa
      241 gcgataactt tcttgaccat accccgcaat ttcaaggaag gctttaaaaa catccctatc
      301 cgtgacaatc ccataaagtt gtccattatc aactactggt aaaacaccga tctttcgaga
      361 catcatcaaa taaatagcgt cctcaaggct agcatactgt gaaacagtca caatatcttt
      421 aatcataata tctcgaattt ttgtcttatt aagaagatag ttcatctcat atatagaaag
      481 actggttgct ttagaaggtt gtgcttctgc cattgtccct tctgtcacta accccactag
      541 ctgatcattt tcaacaactg gcaaacgcct taagtggtgt tctctcaata aatcagcagc
      601 ttctgcaact gtagtatctg gagagacata aactaatttt tttgtcataa aatctttcac
      661 tggcatacct gactcctcta aatattatcc acctaagtaa gctttacgaa cttgatctga
      721 aactagtaat tccttacctg tccctgataa aacaaccttc ccagtctcta gaacatatgc
      781 tttatcagca atcgtcagag ctttatttgc attttgttca actaaaagaa ctgtagtccc
      841 ttgttttttg atgtcttcaa tgatattaaa aatttcttga ataaaaattg gagctaggcc
      901 cattgacggt tcatctaaaa gtaataattt aggacgactc atcaatgcac gccccatagc
      961 gagcatttgc tgctcaccac ctgataaggt tgctgcatct tggtttttac gttcttctaa
     1021 gcgaggaaaa cgatcaaaca ctttttttaa cattttttga ttttgttcac gatccttttg
     1081 aagaaaggct cccatttcta aattttccat aacagacaaa cttgagaaaa cgtggcgccc
     1141 ttctggaacc tgagctaaac cattgccaac aatttttcta gctgctaact tatgtatagg
     1201 tttccccata aaagaaatag aaccctgact tggtcttact aaaccagaaa ttgtcctcaa
     1261 aattgatgtc tttcctgcac cattggctcc aattaaggtt acaacctcac cttggttaac
     1321 ttcaaacgag acatcattca ccgcttgaat aacaccatag tgaatagata gattttctac
     1381 tttcaacatg gtcattatag ctcacccccc aagtatgctt ctataacacg tttattattt
     1441 ttaatttctt caggtgtccc gtgagcaatc agacgaccat attccaatac ataaattctc
     1501 tctgttactt gcataaccaa gttcatatca tgttcaatca gcataatagt aatatcaaaa
     1561 tcactcttta tttgactaat taactgcgtc aattctgcag tttcttgagg attcattcct
     1621 gccgcgggct catccaaaaa caatattttt ggttctgttg ctaaagcacg aacaatttct
     1681 aaacgccgtt gttttccata agggaggttc ttagcaagag catcttgata tgctttcaaa
     1741 ccaaaaatct ctaatagctc cagtgccttt ttccttaagg ctttttcact atgatagtat
     1801 ttgggtaatc gtaaaaaact agcaatagga tgtgataagt gatgattact gagtcctact
     1861 agtacattgt ctaaaacagt catattttta aaaagtcgga tattttgaaa agttctacct
     1921 aaacctaaag aagctatttt agctggtttc ctcccattta atatcttacc atcaattgat
     1981 attgtccctt tactaggaag gtatactcct gttaagagat taaaaagcgt tgtcttaccc
     2041 gcaccgttgg ggccgattaa gccaattaat tcacctttat gtaatttcat cgaaacatca
     2101 cctacagcag ttaggccacc gaaatgttta cttaaatttt ttacttcaag aagtgccatc
     2161 taattgcctc ccttactcgt cttacgaaat agatgagata gagttaactc tttcgttcct
     2221 aataaaccgc taggtctaaa aatcataatt aaaatcaggg ctaaagcata gataatcatt
     2281 cgaacagaag cgtagtcttg tagtaacata ttcaataatc ctaataaaat tgctgctaaa
     2341 actgtccctg tcattgaacc taaaccacct aaaacaacaa taattaaaac attgacagac
     2401 atcataaatg aaaagtcttt tggcataaca gttccaacgt agccagcctg taaactacca
     2461 gcgatagaag ctaaaatagc tccaaaaaca aaaacaatta ctttcacttt tgttgtatcc
     2521 acacccatag actctgctgc aatttcatct tctcgtatag caattgtatt acgccctaag
     2581 ggactacgca aaaaattcat tgcaaggata aggctcacaa ctgccacccc ataaactaaa
     2641 gaccaggttg taaaaggcgg aatccccatg atacccgcag caccattcgt aatatcacct
     2701 ccattaacca acaaaatacg gataatctca gcaacaccta aagtagctat agctaaataa
     2761 tcacctttta gtctcaaagt aggaattcct acacctaagg caatacctcc tgcaacaaga
     2821 gttccaacaa gtatagctat caaaaaacct acataagttg gcattttaac gccgataata
     2881 gcaactgcat aagcgccaat agccatgaaa cctgcatgtc ctagtgaaaa ctgtcccgaa
     2941 caacctacaa ttaggttcaa accaactgct aaaataacat taatcccaat tgtttcaata
     3001 atttgaatat gatagaggtt aaatataccc atagatatta gcacgctaat caagagataa
     3061 tctaacacta ctatagctaa ccaagataaa attgatttca tattttcttt catgcctaga
     3121 ccttctcctt aatatttttt ccaagaattc ctgctggacg gatcaagaaa atgagaatta
     3181 aaatagcata aacgatacca tctcgaaaat ctgacacacc aagtgcagtc gctaaagttt
     3241 ctaaaatgcc aataacaaaa ccaccgatag ccgctcccgg aatgatacca ataccaccta
     3301 agacagctgc aacaaatgct ttcaaccctg gagttacccc cataagaggt tgaacagaat
     3361 tataataaag cccaatcagt actccacctg ctccagctaa agcagacccc agcgcaaatg
     3421 taaaactaat ggtacggtta acattaattc ccatcaattg tgcggcatcg ctatccactg
     3481 ataaagctct catagctttc cccattttag ttttttgaac gatgaactgt aaagtaagca
     3541 ttagtaatag cgcaatacct aaaatgatga gctgaacatt ggttatagtt atcggtccta
     3601 aattatattt aacagtatga atggcttgcg gaaatgccct agtatccgct ccaacaaggt
     3661 ataccatccc gtactccaaa agaaaagaaa caccaatagc tgtaattaac gcggcaattc
     3721 tagttgattt tctcagtgga cgataagcta aatattcaat tacaacacct aggaaagcag
     3781 accccagcat tgcaattaat aaagctagga aaaaattaag atgaagatga ttaatcaaat
     3841 aatagcccat gaaagcgccc atcatataga tgtccccatg agcaaaatta atcagcttaa
     3901 taatgccata taccatagta tatcctaagg caagtaaagc atatatggat cctaagataa
     3961 gtccattaac gagctgttga agcatattat aaccatactt tctattatat attattgtcg
     4021 aattattctg aaaaattact ttttaaaaaa ggggagctga aaattaaact cccatttttc
     4081 tagagaatac ttattttgct gaaataatgt taacgctact tgtttttcca tcctctaatt
     4141 tgacaacgta agctgattta acaacattgt gatttttatc aatagacatt ttccctgtca
     4201 cacctttaaa atctttcagc ttagctaaag cttttttaat atcaattgaa gttttagctc
     4261 ctttagcaga tttagctgcc atatacacag cgtcgtatga taaagccgag aacatggatg
     4321 gttcttcacc atatgcctta acgtaatgat catggaaagc tttagctttg gttgatcctt
     4381 gtgtagtgaa acctgtcaaa taataaacgt ttgaagctcc tacaggtgtt gccgattgca
     4441 caaatttcgg actatcaaaa ccatcaggcc caagaaccgg tttagagata cctaaatcac
     4501 gcgcttgctt aactattaat cctgtctcgg tatagtaacc tggcatcaca atagcatcat
     4561 attctttccc tttcaactta gtcaatgacg cttggaaatc agtatcacca gcggagaatg
     4621 tcatactatc aacaattttt ccactataac tttccttaaa tgattttgct accccctttg
     4681 agtaatctga tgaattatca taaaatagaa caactttttt agctttaagg ttgtctgtag
     4741 catactgaga caatagcttt ccttgataat tatcgacaaa agtagctcgg aagaaatact
     4801 gatttaaggt ttttccatca gaaccataga ctaaattgtc ttgtgtcgca gcaggtgcaa
     4861 tcattggaac tgctgctgca tttacttttg gagttgaaga tgcagctgca cccgatgttg
     4921 ctggtccgat aatgacatta gctcctttgg taaccaagtt tgtggcaacc gttgctgcct
     4981 ctgcattttc tgatttatta tcctgtgata ggacttttaa cttcttgccg ccaacccctc
     5041 cagcagcatt aatttctttt actgctaagt cagctccatt cttcattgaa tttccatacg
     5101 aagaaacgtt tcctgataat tcccagttat accctacctt aatatctttc cctatttttg
     5161 ttcctgtcga agatgctcct cctccgacat ttccacatgc tgctaaaaga actgtactag
     5221 ctaaaacaag cgctcctaaa ctaagtctct tttccatatt tttctccttt tttgaaaatg
     5281 gtaactaaaa taatacagaa ttatcagaat attgtcaata gtttttaaat tgtttttttg
     5341 aaaattatag aaactaaata aaaaagaagg acatctgaac tccttctttc taataactac
     5401 tagtaaatat cttaacgata taagtttcca acaaaatcct gatcaatatc atcaattgct
     5461 gataaccgaa catccttaac aaattttaaa tgttttatct ctttactaac gctaacaata
     5521 tcctctttat taacataaat gactagatat cttaatctac gagaatgata atgaacatct
     5581 ccgtatttat ataatttacg agcgtcacga ttatagtaaa gataaacgct cattcctaaa
     5641 cgctcttgtt tattcaataa ttttttcctt tctgcgtcat atctgtaatt actaagatta
     5701 taacataatt ccaacaaata aaaaaagagt tggtaaactc aactctttta aatctattat
     5761 tgtaaattat tattttccat aatagcatca ataaagccat aatcaagtgt ttcttgagca
     5821 ctcatccaac gatcacgctc tgcatcatca tggacttttt caatagattg accagaatta
     5881 tcagctaaga ttttttctaa agtatgacgc gtttttaaaa gatgctcagc agcgatagcc
     5941 atatcagatt gctgtgtacc tccgcctgtt ccgcccattg gttggtggat catatattct
     6001 gcattcggta acataaaacg ttttccttta gcaccacttg aagcaataat ggttcccatc
     6061 gaagcagcca tccccataac aatcgtctgt acgtccgatt taatgaagtt catggtgtcc
     6121 acaatagcaa gtccagccga tactgaacca cctggtgtat tgacataaag gtaaatatcc
     6181 tttgtattat cttgtgcatc gagaaacaat aactgtgcaa tgatactatt ggccatatta
     6241 tcctcaactt ggcctgtcaa cataataata cgatctttta aaagacgtga gtaaatatca
     6301 taagaacgtt caccacgact tgtttgttca ataactactg gaatcatatc tttctccatt
     6361 tctttttatc actgtcacta ttataatcat ttggtcaaca aaggtcaaat aataactttt
     6421 ttaagaaaag gctttctgag ttcttcagaa aagccttatt catctcaaat cttattttgt
     6481 accaaataag cgatccccag catctccgag tccaggaaca atgtagccat gttcgttgag
     6541 tttttcatcc aaagcagcgg tataaatatc tacatctgga tgtgcttctt gaagtgctgc
     6601 cactccttct ggagctgcta ctaaacaaac gaatttgata ctagctgcgc cacgtttttt
     6661 tagagaatct actgctaaaa tagcagaccc acctgtagca agcattggat caactacaaa
     6721 aatctgacgt tgatcaatat cttcaggtaa cttaactaaa tattcgactg gttggaaagt
     6781 ctcttcatca cgatacatac cgatatgacc aactttagca gctggcacaa ggcttaagaa
     6841 accatcaacc ataccaatac ctgcacgtaa aattggaaca attgctaact ttttcccagc
     6901 taattgtttt tgtacagttg tcgctactgg tgtttggatt tcaacatctt ctaaaggcaa
     6961 atcacgagac acttcatagc ccattaacat cgcaatctca tcaactaact cacgaaaatc
     7021 tttagtagat gttgtagtgc gacgtaagat tgacagttta tgttgaataa gtggatgtga
     7081 aatgacttga aattttccca ttataatgac cgctttctat ttaattttga gcattaaaaa
     7141 cattctaatg cttgtttaca ttcaaagtat tataacataa aaaccctttc catgcgactt
     7201 tttttatagt ctagtcattt tttctaaagt ttgtcactaa tcgcaaaact gcctcctcaa
     7261 taacagacct aggagctgcg acattgattc tagcatgttt tttcccttct tttccaaaag
     7321 ttaaaccatc atttaatatt aatttggcat catatcgaag tttttcttga atttctaaat
     7381 gcgttaaatt ataagcagaa aaatctaacc aaacaagata agttccctct ggtttcatca
     7441 ctttgatatg agttttttgc gttaattgct cataaaggta ttctattgat ccttctaact
     7501 ccatttttag ggcctttaac cattgtttct cttttgtaaa ggcaacttct gtagctaata
     7561 accccaatga agaaatttct tgttgattgt tagctatttg cctcttctta aaatccgagc
     7621 gtaacttttc attttcaatt attgcaaaac tatttttagt ccctgcaata ttaaacgttt
     7681 tagttgcact agataaaata acactcaatt ctttaaaact agaatcaaca gtattaaagc
     7741 tatggtgtac attatcaaat aataccaaat cttgatgtat ttcgtcagaa acgaggataa
     7801 cattatatcg tttacagata tctcctattt tttgaatttc tccttttgtc cagacacgcc
     7861 caccgggatt gtgtggacta caaaaaatat aaagctttac attattttca atgatatctt
     7921 tttctaattg tttaaagtca atctcaaaat actgattatt attaagtaaa ctatttgaaa
     7981 ctaaatgacg attattgtat tttatagttc tagcaaaggg cggatagact ggggtattaa
     8041 ttagaacagc atctccctcc tttgtgaagg cttgtatagc aactgaaatc gctggtacaa
     8101 ccccatcaat taataataat gattttttat caaattgata accatgttct tgcacttccc
     8161 aattagagat agcttggtat aaactgtctt taggactatc atagccaaaa accatttggt
     8221 gtgaaaaatc ttcaatagcc tccgacattt ctggaaagat aggaaaatcc atatccgcaa
     8281 tccaaagggg aaggatttct tgatcttttt gaaccgcctt ccattttatg gtattacttg
     8341 aaaaacgttc tggtaaactt gtgaaatcat atgtaatcat aattactcct gactattcta
     8401 cagctaaaaa tatcccttgt cgccttggtg actattttac catttgtttt tgatactata
     8461 tactaattaa cattctaaag ataaaagtga ccattttaaa cgatcacttt ctttattatt
     8521 ttaatttatc attttcataa gtatggctta attctaaatt aggaaagctt tgctgcctaa
     8581 gggcttcata aacaatcata caaacagtgt ttgaaacatt caagctacgc acatgctcat
     8641 cgttcatggg aattcgtaag gccttttcgg catgctcgcg cataaaagtt tctggcaatc
     8701 ctttatcttc acgaccaaac ataaaataat ggtcttgatc atcattgtaa ttttcatctg
     8761 agtaaacctt gtcagcaaat ttgctaatta ggtgaacttt ccctcgacat gacaacataa
     8821 attcttccaa accatcatag aaactaacat ctagcttatc ccagtaatcc agtcccgctc
     8881 gtttcatttt tttatcatca ataggaaatc ccatcggtcg tatgatgtgc aaaggagcat
     8941 ttgttgccgc acaagttcta gcaatattac cagtatttgc tggaatctgt ggttcaaata
     9001 aaacaatatg atttcgtccg ctatcggaac gatgattttt ctgcgttagt gtttcaatat
     9061 tcatctcatc atcactttct agaactcatt ctgactcgca aaaaaaccac actgctcgga
     9121 gtcaatctca gcaaacaatg tggtttatgg tggtttgtta acttaaatca taacaggttt
     9181 gatttaaact aacaaatcgt tgacactatt atagcacttc agtctacaaa gtcaacctat
     9241 tcatgagttt ttgggttatt tttttgatag atataatgac aaatcaaaca gaataataag
     9301 caaaaacatt ggaaaattaa aaccgtaaag aaattactta acaaactccc gataaggagg
     9361 atcaaaccgc ctaaaattgc tagaatcggt gaaactaagc cggtaaattt atttttaata
     9421 tcttttttga gataaaggct gagtactaaa acataaagaa tgattaaact ggtattatta
     9481 aacaccacag caatttcact aatatcacta cccggcaata agttaaattt tgtaaccata
     9541 aaatgaacaa aaagccaaaa aatagctaca gccgtcaccg tcaaactagc tggcaatgac
     9601 atttgatact tcaaatttat gttagccatc ctctcacttt tgatccatcc gcgttccgca
     9661 aaagcttgag gtagacgcat cgtcccaagt aaaagtccat tggcaacacc taatacagat
     9721 agaataacaa tgaaagataa taaacgccca acactaggac cgaaaataat attagctgca
     9781 taattaatag catcattacc ggttgtcata ataaagctag cacctaaaat ttgtgtcaaa
     9841 ccatagaaaa atgccaaata tgataataag ataagcgttg gaccaatgac aaaggcaagt
     9901 ggtaaattct tcttgggatt cttgacttct ggtgcaatat taacaaaaat tgtccaacca
     9961 tcatatgcaa aatacaatgg taccagacct gacacccaac tccatccaac atttgacggt
    10021 tgaatagctg ttaaatgttg tggaagttgt ggagctttat cagaccaaaa agcaccaatt
    10081 aaagcaatta gaactaaagg aatcattttt acaaaggtcg ttaaagattg gaatccccct
    10141 gcaatacgct tagcaaagat attcatgaga ctaagcgcta aaatataaac agcagctaaa
    10201 ataatctgtg attccaaact tgatgattca cctaaagtat aaaatgcagc tacccatgaa
    10261 acgattgctg ttaaagttgg aagatacaaa aaagacgcaa acaagcctaa agttgctgct
    10321 aaagctgggc taacatattt ttcgtagtaa gaaaaaatac caccactttc actcgttcta
    10381 atagctagct cagaaattga caaacttcca aaaacaatcg aaaaagaacc tagtactaaa
    10441 attaccatgc ctaaaaagac atctccacca gtaaatttaa ggatgtcatc aactttaaaa
    10501 taaatgcctg aaccaatcac tacacctaca atcatagcaa ttgtagtcat taatccatag
    10561 gtctgtttaa tagaattatg attctccata atatctccat ttctactgta cgcttaaagg
    10621 ttaattataa cctaatttag ggcttgtcac aagtttatct gtgttatttt tcaccagatt
    10681 ctgataagag aatcatatta ctcttatcag aaagatgaaa acgtttttta accataattc
    10741 tcgtgcattt ttactaaaaa aaaggtatca tagagtttat tattggaggt aaaaatgaga
    10801 ataattgttg tcggcggagg taaagtcgga accgcacttt gtcgctcact tgttgctgaa
    10861 aagcacgatg ttgttttaat tgagaaaaaa gaaaacgtcc ttaaacgtgt cactaaacag
    10921 catgatatta tgggaattgt tgggaatgga gcaaactata aaattttaga acaagccgaa
    10981 gtcaaaaatt gtgatatttt tatcgctatc actgatcgag atgaagttaa tatgatttca
    11041 gcagttcttg ccaaaaaaat gggggcaaaa gagactgttg ttcgcatgcg taatcctgag
    11101 tattctaatc cttacttcaa agataaaaac tttcttggct tctcttcagt tgtcaaccct
    11161 gaattgttag cagcacaata tatcgctaat accatagaat ttccaaatgc aacttcagtt
    11221 gaacacttcg ccaacgggcg tgtgatgtta atggaattta aaattttaga aggaaataaa
    11281 ctctgtcata cttcaatgag tcaaattcgt aaaaaatttg gtaatattgt tatttgcgca
    11341 attgaacgcg atggcaaatt gattattcct gatggcgatg caactatcca agtcaaagat
    11401 aaaatctttg taactggaaa tcgtattgaa atgatacttt tccataatta cgttaaaaat
    11461 aaagttgtca aaaacttgat ggttatcggt gcaggacgca tcgcctacta cctactcaat
    11521 atccttaaaa ataccaatac acacgtcaaa ctagttgaac tcaaccaaga gcaagctgaa
    11581 tattttagtc aagaatttcc caatgttcct gtcgttcatg gggatggtac tgctaaaaat
    11641 attttgttag aagaaagcgt tacaagtttt gacgcagttg ctactttgac tggtgttgat
    11701 gaagaaaata tcattacctc aatgttctta gaaagtatcg gtatccctaa aaacattacc
    11761 aaagttaatc gaactagcct actagaaatc attgacgata aacaactatc aagcattatc
    11821 acacccaagc gtattgccgt agaccatgtg atgcactttg tacgcgggcg tgtaaatgct
    11881 caggattcca acctagaagc tatgcatcat attgctaacg atcgcattga aaccttacaa
    11941 tttgaaataa aagaaactag caaattagca aatcgtagcc ttgcatcttt aaaattaaag
    12001 caaaatattt taattgcagc gattattcgc aataataaaa cgattttccc aactggtgag
    12061 gatgttttaa cagtgggtga tcgtatcgtt gttatcacct tattaaaaaa tattactaga
    12121 actagtgata tgttagcgag gtaggaatat gaataaaagt atgattcgtt tccttctctc
    12181 aaaacttttg ctaattgaag ctgctcttct agcgattcct ctaactgttg gacttattta
    12241 ccgtgaaccg caaagtgtca tgatgagtat agtcattacc atgattattt taatcatcct
    12301 tggattactg ggttctctct ttaaacctaa aaattatcat atctacacta aagaaggcat
    12361 gttaattgtt gcactctgct ggatactttg gtccttcttt ggcgcccttc catttgttat
    12421 ttcagggcaa atccctaata ttatcgatgc tttctttgaa gtatcttctg gttttacgac
    12481 aacaggagca actattcttg atgacgtcag tgtcttatca cccgcgctct tattttggcg
    12541 tagctttacc catcttatcg gtggaatggg ggttttagta tttgcccttg ccatcatgga
    12601 aaattctaaa aatagccatt tagaggttat gagagctgaa gttcctggac ctgtatttgg
    12661 taaagttgtc tcgaaactta aaaaaactgc acaaattttg tatttgttat atcttcttat
    12721 gtttgcagta tttgcagtaa tattatattt tgcaggtatg cctttctttg atagtattat
    12781 catagcgatg ggaactgcag gtacaggtgg ttttgcagtt tacaatgata gcattgctca
    12841 ttataatagt cctctgataa ctaacttagt ttctattggt atgcttatct tcggggttaa
    12901 ctttaatctt tactatcttc ttttactccg aaaaattaaa gcattctttg gtgatgagga
    12961 actaaaaacc tatctccgca tcgtggcaat agcaactttc atgattgctc taaacgtcat
    13021 cggtatgtat gataattttc gacaaggcct cgaacatatt ttctttgaag tatcagctat
    13081 tattacgaca actggttttg gcgtgactga tatcactcgt tggcctttgt tttcacaggt
    13141 tattttgcta ttcttgatgt ttatcggtgg ttctgctggc tctacagctg ggggctttaa
    13201 agtcatgcgt agcttaatcc tagcaaagat tgcacgtaac caagtattgt ctacactcta
    13261 tcctaataga gtcatgtcac tacacattaa taaaagcgtt ctagataaaa atacacaaca
    13321 tggtgtttta aaatatctaa ctatctattt agctatcttc atggcactcg ttcttgtttt
    13381 aacgcttgat actaatgatt ttttggttgt tatcagtgct gcagcctcat gttttaacaa
    13441 cataggccca ctcttaggta gcaatgaaac tttctcattc tttagtccat tctcaaaact
    13501 ccttctttca tttgctatga tagcaggacg tttagaaata taccctgttt tattgatgtt
    13561 tattcctaaa acatggtcaa aactataatt acaaataata aaacggatat atcaatctga
    13621 tatatccgtt ttattacgtc ttaagctaaa atgatcaggg acaggatcat ttcctccgtg
    13681 ggctaaggga tgacatcgca aaatacgtgc aatccccatc aacacacctt ttagaccatg
    13741 tttttgaata gcttctatca tatacgtaga gcaagttgga cgataacgac agctagctgg
    13801 gaaagctgga gaaatatttt tttggtaaaa gcgaactaag aaaatcaaaa aagatttaag
    13861 cattaagatt tattaatagc cgcattatgc aactgactaa tttctttttt atttaatcgt
    13921 cgtgcttccc caggacggag acccgttaaa tctaaggtac cgaattgtgt acgagatagc
    13981 ttgtccacta ataacccaac ttgttcaaac attttcttaa cttgatgatt acgaccttca
    14041 tgaatagtta attcaactac tgaacgattt ttttcatgat ccacttttat gatagtatag
    14101 cgagcaggtt ttgttttctt accatcgata acaacgccac gcgttaatgg tcgaaggttt
    14161 tctttagtag ctattccttt aacacgtgct aaataaactt tatcaatttc attacgagga
    14221 tgaatcattt tatcagtaaa atcaccatca ttagtcaaaa ttaaaagtcc agttgtatcc
    14281 caatccaagc gaccaacagg atatatccgt tctttgactt gtggcaacaa gtcaataact
    14341 gtcttacgtc ctttatcatc tgagacactt gaaataacac cacgtggttt atttaagaga
    14401 taatatacct tctcttcgtt gtaaattgga cttccttcaa tttcaaccag atcaccagcc
    14461 tttacttggg tcgccaattc attaaccact tgaccattaa tcgtgaccat tccttgcttg
    14521 attaattcct cagcttttct acgacttgca atccctgcat gcgcaatata tttattaatt
    14581 ctcattgttt tccttatctt ctgtattaat tgaatccatt gaaaataacg atacttcttc
    14641 gtcaactaat tcaattgaag aggcgtcaat taagtcatct aactgattaa ttcccatata
    14701 atctaagaaa taattagttg tctcataaag gttaggacgt cccaacactt ctttctttcc
    14761 agcctcttta attaaaccaa aagctatcag tctcgttata gctccactgg aattcacgcc
    14821 acgaatatca tcaatttcaa tcctagtaat aggttgttta taagcaataa tagatagaac
    14881 ttccaaactt gcccgagata gactctgatt aatcggagcc ttcgcataat ctctcaacaa
    14941 agtcatataa ctatcctttg ttaccaattt ataagtttgt gctgtctcaa gcaataatag
    15001 actagaatcg tcatcttctt catatcgttt agccaacttt tctagttgct gtattaatgc
    15061 tgatggtgtc aaagacaata attctgccat ttgacgcaaa ctcagtccat cttcacctgc
    15121 aacaaaaaga agagcttcta ttgagcctaa ataagtcatt tctcttcctt tcttaagata
    15181 acttgactaa aattactgtc ttgttctact gtgatttgat gtaattttat caactctaac
    15241 attgccaaaa ataaagttat catttcagat tttgtttggc aatccgcaaa cacttcttgt
    15301 aatgtcgttt tctttttaag attaaaatgt ctttcaataa cgatcatcat atcttcaata
    15361 cggtagtctt ctttctcaat taccgtttga ctatttgaca attctttttg tttttgggac
    15421 atcatttgag aaaaagtcaa aaaaagatcc atcaccgatt tatcgtgtag aagaattgca
    15481 tcttcaaaga taacttcttg ttttggtttt gaaaaatatt tggcgcgttc ctgatgctga
    15541 ttagctaact cttcacttag cactttaaaa cgtctatatt cctctaattg gctcaacaat
    15601 tccatttctg gatcatcttc aataggattt gactccacta ctttaggtaa taagttacgg
    15661 ctttttatca acatcagctg acttgccata agcatatact caccagctac ttctagacgc
    15721 atcgcctgta atgttgctat ataagcaagg tactgctcaa taacttccac aatcggtaca
    15781 tcgtatatat caacctcata tttagatacg aggtgcaaga gtaagtctag gggcccctcg
    15841 aaatctttta acttaatatc cattatctat aatatctctc taaagtgata ggagttttta
    15901 agcctagaag gcgactgagc tccgtcatcg ttttgccatt ttctttttgc tttagaataa
    15961 attgttctcg cagaagttgt gcagttagtt gttgctcatt cttctcattt aagaaatctg
    16021 ttaattgatt aaaaaaccat tgtctagaat aaggcttacc gttatgctca aagactaagt
    16081 caccatcttc ctctaaagat tccaaaagaa aaggaagcaa gtcttctgga agttttaaaa
    16141 tacgcttcat ctgcgatttt tcaatactta agatattaaa agttgtatca aaatcagcct
    16201 tcttcaaatt agcaatttca gccggagtta aacctaaaga taaaatcaac aaagcaatca
    16261 aacgaccact cggataatca gtatcttgat agaaatttga taaatccatt atttgagact
    16321 ttgtctgagc caaagtgatt ttttcagtct cttgtagtct gtaaaattcc ttcagcgttc
    16381 ctctattata taaaaagaaa agaaattgat tgactgcaga taactttcgc ttacgagctg
    16441 ttaacttaaa ttcacttaga gactgttgat acaatgccag tttttcagaa tttacatgtc
    16501 caccaattat tttataaaat tgctttaaat catagtgata ggacttacgc gaattgagag
    16561 ataatttctt actctcaata aaattattaa tatcatttat cattgttttt aggagtgata
    16621 gtatattcgt ctgtaaaatc atgtaggagg ctattaatag cttttaaaat cgatttacgg
    16681 gttataatgc ctctaaaatc attttgatca ctgataacag gtaaaaatgg ataatctaca
    16741 atcttatgca taattgctgt cagagtagct gcctcattaa tcggctcgat ttttgtatta
    16801 accatctcaa caatatcagt ctgtgccatc tcccaatctg ttaattgacc tttagattgg
    16861 tagcccataa tgtctgagat actaatagta cctacatatt ttttctcttt agtgataact
    16921 ggtacacgtg aaaaaccatt acttgctaat agtaacataa cgtggtctgc attgtgtgta
    16981 tccacaaaaa tagcaacgtc ttcagctgga attagatagt ggtccaaatg atttagcaaa
    17041 aacgactcaa attctttagc aatcatcttg ataattcctt tgtcaagtta ggatagggtt
    17101 ggtgttggcg agtataatac tctaccaaaa ccttactatc tgttatagtt acaacagcgt
    17161 aaagcttctc atttattggt cctcttggct gcaaaacact acctggattg ataaaaatag
    17221 ttttaccatt tttccaagca tctgcacgat gaagatgacc atataaacaa atatccgcgt
    17281 cttcttgctg agctaataaa tccaatttat cccaggtaaa attaatctga tggagatgac
    17341 catgggtttg aacaataaca gcattatcta tttttgtaac caaaacttcc ggataacctg
    17401 aatcataatc acaatttcct gtgaccacat gaatgccttc ccatatagga tctgagctag
    17461 gaagttcgga atccccattg tgaaaaatag catccacttt tcccaaataa tgattcttta
    17521 tatcttttac aatgtcacga tctccgtgag aatcactcat tacaactaat tttctaattg
    17581 ccattttgga aatacctcca tcaatttacg gacagcttgc ccacgatgtg atagattatt
    17641 tttttcttgt gcgctcaatt cagctgctgt cctactacct tctccgacaa taaagagcgg
    17701 atcataacca aagccatttt cccccttagg ttccgtacca atataacctg gccactctgc
    17761 ttcaactacc aaactttctt tatttggagc tgacacgact aaggttgtat gaaactgtgc
    17821 tgaacgtctt tctttgtcaa ataccatggc taattcatgc aataatttgg cattattacg
    17881 agcatctgtc gcgtcaggac ctgaaaaacg agcagaccat acccctggca aaccacctaa
    17941 agcatctact tttaatccgg aatcatctga aattaccatt tttccagtta attttgaaat
    18001 agtctctgct ttaagtcgag cattttcttc aaaggtcata ccagtctctt caacttctgg
    18061 taaatctggg taatcattta aattttcaac tttgagtcct aatttcccaa ataattctct
    18121 aaattccttc gtttttcctt cattgtgagt cgcaattaat atggtatccc caaaattatt
    18181 atcattcttt aaatcaaaga atttttctaa agaagtgccc tctttaggta agtgaactaa
    18241 gaacaactga tcatcttcac taactaagat ggccccttta tgttgagtca ctttgatatg
    18301 ccttgaatac tcttgattaa tcatatcaac gaggaatgct aataattgaa aatccgaagt
    18361 catctgcaca acgtgaagct gtagaccttc aactttcaaa gttgataata gacgatgaaa
    18421 aacatcttgg atagcttcgt aagattcatc attaccaaaa caagtaaaat aattaaaagc
    18481 ttggaaacta cctaaaaacc aattgccttc agttttactt tcaaaaattg tttttgtcat
    18541 aatacgatac gctccacatt tatttcttga ctaagccatt gttctgcaat ttctttaaaa
    18601 ccttttgggc tggcggttgt gtaaaagtga tgaccaccgt gtttattttg ccaattatgg
    18661 tttatctcaa aatagttcaa taaaacagaa atatcacgaa cggtttctgc gccactatca
    18721 attaatttaa cctcagcccc cataacattt tgaatgatgg gacgtaatag gggataatgc
    18781 gtgcaaccta aaattaaagt atctaattta ccaactaatg gggacaacgt ttcataaacc
    18841 acctttttgg ctaaactaga agacatctga tttgattcca caattggaac aaatttcgga
    18901 caagcaaggg ataccacagc agtatttgga gacaaagctt gaattttttg acgataagca
    18961 tctgatttaa cagtcatggg agtacctata ataccaactt tccctaaatt agttgatttg
    19021 atagctgcgc tagctcctgg taaaataacg cctaaaacag ggatgtctag tttttcttta
    19081 atttcttgcc aggcaactgc agttgctgta ttacaagcta taacaatcat cttaacattt
    19141 ttagtcaata agaagttaac catctgccag gtaaactctc taatctgttg agcaggtcta
    19201 ggaccatacg gagctctagc ctgatctcca atgaagatta cttcctcttc tggaagttga
    19261 cggaacattt ccttaacaac cgttaaacca cctacaccag aatctagaaa gccaataggc
    19321 ctactatcca tttttattcc tccaatatta gtgactaaaa gaggctggac aacagtccta
    19381 gcctctctat acaataacca atcagtcaaa tctacacttg accaagtttg cccaaatctt
    19441 gtctagaaat ctgatggact atcactcact atttcatcac taaacgcttc aatgctaaaa
    19501 cactcttatt ttttagtttt gatagctgtt tttgcgtgtt ttacaatatt acggtatgtt
    19561 tgttgaactt tagcttcact aggtttttgt cccatttgac tcatcatttc acgaatagca
    19621 tccggtgtta aacgagggtg ctctccaatc tctttttcaa tttgtttacg agcaataaaa
    19681 atgcctccca cgagaccacc aaacaaagcg acgataatta ataaaatcca aatagttgtt
    19741 gacatctatt cattctcctc atttatttac atgactcatt atagcaaaaa aagaaagagt
    19801 ttacaaatat atgtaaacaa aatcaaagcc tctatcgtcc ttgtcacgtc aatacactat
    19861 taaattttca ctagagcatg gttaagcaca tagtcgagtc ctttatctta tcctaagtgt
    19921 tttccccaac ttttgtgtca tccatttcca aatgaagaga gctatgtaaa aatctaccaa
    19981 tccatgtagc aaagagccta gtcctatcaa agaaaatagt gaccataaat aagttgaact
    20041 actgttagaa gtcattgtga ttatataaac agtaataaat tcgagcagac catgaattat
    20101 gttaatagct acagcaaata acaatgtttg ccatttactc atcaataaag agggctttga
    20161 cttaatcaag aaagccgcaa ttaatgcaaa tccgatatgt gataaagctc taagaacgat
    20221 tacaatcgga aaaccagcta gtagaaatcc aagtcctgtc cctaaagcta ccaatattgc
    20281 aactggaaca gatataaaaa tacttaaaaa taaaggtaca tggcttgcta aggtgaaaga
    20341 agcaggccca ataattattt tagcaggcat gattatagga atcatgattg caaaagctac
    20401 taaaatagaa gtataagaaa ccatttgtgt tgtttttttt gacattttta tctcctttga
    20461 agctattata caaaataacc ttacaggtgt aaagacacct gtaaggttat ttgtctatgt
    20521 ataaaattcc tgcttttttt agagcctcac atatttggtc aaaaactgac tgatcttgac
    20581 aagaaatcat atgtgtatgt agaccatctg tcaaagagga tagcaattct gcattggaaa
    20641 cttttaattt agcagtaaaa ttatcaatgt ctttttggct ttgaatgttt aagggagcag
    20701 tcagcatccc ataaataggg tgctctacct ccacattcat aatgattcct tgataccgca
    20761 aaataatttc cagttcttct tctgtctgtt ctatgccatg ctgacaaacc aatctagctg
    20821 tgaattgatg agtacttaga gcactggaca taaggtatcc ttttggggta gaaataatat
    20881 cacattgttg tgcacgcaat aaggcaatat ctccaacaat cacctgccta ctaacactaa
    20941 aaatctttgc taatgtgctt gccgatatag cttcttttgt tccttttaaa gtcgttaaaa
    21001 tattttctcg acgctcctgt gccttcatat aacaaattcc ttctttggtg ttatttcaca
    21061 attaaaaact ggctagaata tctaaccagt tagcctcaat catttcgtgc aaaaagacga
    21121 agaatattta agaatagatt aataaaatca agataaaggc ttagtgccat cgaaactgcc
    21181 caaccatctg cgacttggcc gccttggcta ttgtagacat atttaatcat ttggttatcg
    21241 taagcaatca atccagaaaa gatgatgaca catacgatac taatgatata actcataccc
    21301 ccactaccga taaataaatt aacaagactt gcaattaaaa tcccaattaa agcagccatt
    21361 aacgctttac gcatacctga taaatctttt ttagtcttag ctccaatcaa agccattgca
    21421 aagaaaacag ccgcagatgt tataaaagct tgaagaactg tagtttgcgt gtaccttgcg
    21481 ataatgaaac ttaaagtaaa cccattcaaa gctgaataaa tcaaaaataa tggtaatgca
    21541 gctggtgtgt ttcgacgcgc cgctccacta gcaacaaaga ctaaaaccaa ttctaagatg
    21601 attgctccaa aatacaaccc tggcattttt tgcatgatgg caatcatatt ttgtggaaac
    21661 atatacagca taatcgcaga aacagcagct gacaaaccta ctcctatccc tacaagaccg
    21721 taaatcttag caaaaaattg attcagccct gaatcagatt gtgtgtaaat gacgttatca
    21781 ttcatatgta ttttcctatt ctaaatttta atgattatga ctacctaaga atttgcgact
    21841 aaaacggata ggtttcttaa aaatacgttt gagtggacga gaagcctctc gtactgccca
    21901 atacttatca accatagtca caatataact ttctttataa cgtggcggtg caatagtcgc
    21961 tccccacatg tgcttcaaaa taatatcttc ttctctagcg tttaaatcag ttaattttcg
    22021 cgcattacga accgctatcc ttggatgaac ccaggcatgg ctcttgttaa atttcgttac
    22081 acgccaatca taataaaaga aatcatgtag taaaccacct cgagcagtac tactcgcatc
    22141 ccaaccaaac ttcttagcaa ttttataact tgtataacta acatttatag aatgctctaa
    22201 gcgagttgag tgatgatgtt ggacaatagc ttcgagtttt tggaaacgtg gatggtcaat
    22261 aaggtgaccg accaaatcca tgaagtcctt atcgtcttga tactttttca ttataatcac
    22321 ccctctgggg atattataac atatgaactt aaattaagct gaaaatgact actcccgcag
    22381 ctactgctac attaagactt tctgcttgtc caggcatttc aatatgtgct aaaacatctg
    22441 cttcttctgt catagttttg gaaattccct gtccctcatt tcccatgatt aaagcaaaat
    22501 aatcatattt agggagattc ttataattta ccgattgttc agataaagtt gtcgcaatca
    22561 tagttatccc ttctgcttta caaaagttaa caaactgctc tatttctacc ctaaatactg
    22621 gtaaatgaaa gtgactgcct tgcatagaac gaagtgtttt ttggttatac aaatcagcag
    22681 atttctgaga taaaaaaaca gcatcaaaat tcgctgcatc tgcagttcga accattgttc
    22741 ctaggtttcc tggatcctgc acatcttcta atacaagaaa acgtcctttt ttaatatctg
    22801 tccatcttgt ctcttgaaag gcaatctcag ctacaatccc ttgaggagtc tgtgagtcag
    22861 ttaattcttt caaaacatca tctgtaacaa caatagctct ctcgggtttt ctcaaacgat
    22921 ctattgccgt ttctgtcaca aaaatattta aaaattgtgc accatatttt tcagcttcct
    22981 caaataaatg ccagccttca ataagatatg aagatttccg atattttttt tgtaacaact
    23041 ttttcgtttt tttaatgaga ttatttgatt ttgaagttat aatagtagtc ataaacctat
    23101 tatatcagaa aagaggtcaa gttatgaaaa aagttcattt aattgtctca gggcgcgttc
    23161 aaggagtcgg atttcgctat gcaacttatt ccttagcttt agaaataggt gatatttacg
    23221 gtcgcgtttg gaataacgat gatggaactg ttgaaatttt agcacaatct acagattcaa
    23281 ataaaatgac acaatttatt cagaaaattc gaaaaggacc ttcaaaatgg tctaaagtaa
    23341 cttatgttga tattaaacta gataacttcg atgattttaa tgactttaaa atgagtaatt
    23401 aaaaatcatt ctgcggaagt attgtttaat tcttaaaaaa aaagtaaaat agataggtat
    23461 attaaaaatt agaggaacta aaaacattga aaaaaacatt gaaacgtatc ttattctcta
    23521 gcttatcttt gtctatgtta ttattattga caggatgtgt tagcgttgat aaggctggaa
    23581 aaccctatgg agttatctgg aatactcttg gagtgcctat ggccaacctc attacttatt
    23641 tcgctcaaca ccaagggctt ggttttggtg tcgctatcat tattgtaacc gtcatcgtac
    23701 gtgtcgttat cttaccactt ggactttatc agtcttggaa agctagctat caagctgaaa
    23761 aaatggcata ctttaagcct ttatttgaac ctattaatga acgactacgc aatgctaaaa
    23821 cacaagaaga aaaattagca gctcagacag aattaatgac agctcaacgt gagaatggcc
    23881 ttagcatgtt tggcggtatt ggctgcttgc cattactcat tcagatgcca tttttctcag
    23941 ctattttctt tgctgcacgc tatactcctg gagtctctag tgcaaccttc ttgggactta
    24001 acttaggtca aaagagtctg acacttactg tcattatcgc cattctttat tttgtacaat
    24061 cttggttgtc aatgcaaggt gttccagatg aacaacgtca gcaaatgaaa acaatgatgt
    24121 atctgatgcc aataatgatg gtattcatgt ctattagttt acctgctagt gttgcgcttt
    24181 attggtttat tggtggtatt ttcagtatta tccagcagct agttacgact tatgtactta
    24241 agcctaaact acgccgaaaa gtagaagaag aatatactaa aaatcctcct aaagcctaca
    24301 aagctaataa cgcacgaaaa gatgttacaa attcaactaa agccactgaa tctaatcaag
    24361 caattattac atctaaaaaa ataaatcgta acgccggaaa acaaaaaaga cggggataaa
    24421 acaagcatac taaataatgg tgtgttgtcc taaacagtta aagattatta tgtaattcaa
    24481 gtaaaaaaga cttagttcta tgtcctacat agctaagtct ttttagtttt gcctaacttt
    24541 tcatataaaa agcaccctaa aagtcagata aaaatctagc ttttaaagtg caagtaatct
    24601 agctaacaga ctaccttatt aataaagtaa tcgtttttcc caccagtctt gaataattgc
    24661 ataaagcgta caaattaccc aatagataag ggctactaag atataggcgg tcatatagtc
    24721 ggagtaggtt cccccaacta ttttagcatt attaaagata tcaggtaccg taatcatcgc
    24781 agtcaatgag gaactcttga ccatatctag caaaacatta cttaaagaag ggagtgcaat
    24841 tcttgttgct tgcggaataa taatgcctct ataaatcgtc ggtgttttta accctaaagc
    24901 tctagcagct tcccactgac catgatcaac agctaaaaga gctgctctga taatttctga
    24961 tatatatgca ctagacatca tggtaaaacc tacgatagaa gcagtcacag catctaactg
    25021 tatccctata aaaggcaaac caaagtagat aagaaagagt agaaccatta aaggaatccc
    25081 acgcattaag gaaatatgaa tattagctag atacctcagt aagcggaaat gagacatctt
    25141 cattagagct acaaagaatc ctacgatagc tcctaaaata aagccaacca aggataatcc
    25201 aatggtatat ggtaacccct gaataacact tggaaaagcc ttaacagcta gttccaaatt
    25261 aaaaatggca tcccagttta tcacttatct actcctctcg aaatttttta tttaacttta
    25321 attactggaa ttttatccgt tccagcaggt tcttttgtga gatcttgacc agcataatat
    25381 ttttcagaga tttttttcaa agaaccgtct ttcttcatat ccttgatagc tgcatcaatt
    25441 tttgttttga ggctcttatc ttttttactc atcacaatac cttgctcagt cggattatac
    25501 ttcacatcac ccatttttac tttgatatct ggatattgtt ttgttacata ttttacagca
    25561 atgacttgtg tgtagtaatc attcggaatg aaatctgtac gtccagttga tacatctcga
    25621 aggtaaacgt cattagttac attgtcataa ataacaggtt cagcaccttg ctgttttgca
    25681 attttcatat attgcgttcc agcgccacca ccagcttttt tacctttcca gtcagataaa
    25741 tcttttgctg taatctttga agaaccatct gcacgtacaa tcatacctcc aacagaatat
    25801 ttataaggtt gagagaaatt gtattttttc aaacgctcgg gagttgtgtc aaagttattg
    25861 acagcaacat ccaccttacc actatctaca gaagtaaatg attcagcaac tccaacttca
    25921 acaaacttaa cttttatctt aagtttttta gcaatcgctt tcatcatatc aatctcataa
    25981 ccagtcaatt ttttatggtc atcataataa cttgttggat aaagcgttgc aggggtagca
    26041 accttaagga caccatcttt tttaatattg tcccatgttg tttctttgct agattgacca
    26101 tttgagcaag ctgctaatgt cattgcagat aaaccgacta aaccaagtaa aattgtcttt
    26161 ttcataagta ctcctgtaga ttaaattata ttaaaatggt ttggcgtact ctatcgaaaa
    26221 ttaaatttaa tgaaaattaa tacataaatt tacgataata atacaaccat tgttaagagt
    26281 accatattgt atccgttttc gcaaaaatat tgtttacttt tcaaattaat ggtaaacagg
    26341 ccctttgaat tgcttctctt tttcaaacaa ctctgcaaca gcaagcaata aatcttctct
    26401 ccctttagcc gctgtcaatt gtacacctaa aggtagcccc tctttagtta gatgagttgg
    26461 tatggctaaa gaaggttgcc ctgtcaagtt tggctgatgt gtaaaaggtg tccaggccat
    26521 tgagtcttca aacattttcc aaattaaatc ctgttgttca cttactgaga attctcccat
    26581 atgcctcaat tgtttctgga gtgtctcgtc taaatcaaat tgtccatgaa aaggagctgg
    26641 ttgattagtt gctgctgtta aaattaaatc ataattttca tgaaacctcg ccatggtagc
    26701 tgcaaattga tcccatgaat ctaagagttt actgtaatct ttagccaata ccctttgtcc
    26761 actctgatac atagcccagg tcattaattc catatcgcta aattccattg aacgccctaa
    26821 actcttttcg atgtcatcaa acattgctgc tgtttcaacg ctattcatca tacaataagt
    26881 tttcatagaa tgaataccat ctaaaggttg ttctactaat tctaaatgat tccctttttg
    26941 agacagaaaa tctgcggctt ctttaatagc tgctttagcc tcacttgata cctttgtttt
    27001 taagggagaa tccatcaata ctgctatttt taggggttta ctaacgctaa actcgaaaag
    27061 actctctttg ctgagttttt tcaggggaaa tgggctttct acttgatatg attgtaaata
    27121 atataatagt cgttttgtat ctctaactga ctttgttaaa gcaaaatgac tagaagcccc
    27181 ttgccaacct ctataggatg aaggtccaac aggtatacgt cctcgactcg gttttaaacc
    27241 aatcaaacca ttaaaacttg ctggaattct gattgaccct cctccatcac tagctcctgc
    27301 aattggaacc attcctgaac taacagctgc ggctgcacca ccactagagc ctccagcatt
    27361 tcttgaatgg tcaaatggaa gattaacatt cccgtgaagt tgaccatcac taatattctt
    27421 aaaaccaaat tcaggggtat ttgttcttcc taaaataata aaccctaatt tttcaaagga
    27481 ctgcactaaa tagtccgttt gtttagcatg atagtgttta aataattgtg aacctgaggt
    27541 tgagagttgt cctttttgat tctgtcccaa atctttcaga agaataggaa ctcctgcaaa
    27601 aggagcattt gattcatttg cgtatttggc tgcttgacgc gcctcattat attgcttgct
    27661 gacaacagca ttaacgctta cattctgttc ctcgatcttg tagattgctt gctctactaa
    27721 ttcttgcgac gaaatcttat gctgttttat cgcttgtacc atagcggtag catctttaaa
    27781 aaccatataa acacctcata aaatgtattt ttattataag ataacaaaaa atacttaaaa
    27841 aaatagaata acgtctagac tctagatatt attctatgat ttatactatt aatttgtttt
    27901 ttcaacacta atgatttcaa cttgatagct accagcaggt gattcaattg tagctaagtc
    27961 tcctgttttt ttaccaatta aagcatgagc aattgggctt tcattcgaaa tttttcctga
    28021 aaaaatgtca gctccagcag caccaacgat atggtaagta tctttatcat ttgtaccaac
    28081 ttcttggaca aggacagttt taccaattgc aacctcatct ttagcaactg catcactatc
    28141 aataatttca gcataacgaa tttttgtttc taaaatttga atttgaccct caacaaatgc
    28201 ttgttcgtct tttgctgcat catattcgct attttctgat aagtcaccat aagaacgagc
    28261 aatcttgata cgctctacaa cttctggtct acgaactaat tttaactctt caagttcttt
    28321 ttcaagttga tctttttcca cttgtgtcat tgggtaagtt ttttctgcca ttttatttct
    28381 cttttctttt cattttttca agtcaaagat ataaacatga tgactcgcac catgtttata
    28441 agcttagtta atttgactat tgatatattt ttcaacgtta gccttgtgtg tttcaaaatc
    28501 tttagcgtag tatacatttc cggtcttcac atcagcgaca aaataaaggt aatctgttga
    28561 agcaggtttt atagtcgctt caattgctga gatacttgga ctatcaactg gtccaggcat
    28621 caaaccagta ttagtataga tgttgtatgg tgatttaatt gaagtattaa tttgagcatc
    28681 ttcagctaaa cttgttttat cacctaattt ccccatagca tacaaaattg caatgttact
    28741 ttgaagagct tgaccggctg ataatcggtt ataaaagaca cttgctattt tacggcgatc
    28801 ttcgtctgtt gatccctctt tttcaacaag agatgataat gtcaaaacat catttacaga
    28861 catattcttc gctttaatag tgttatagta aggtgccatc ttagtattca ttgttgaaat
    28921 catatcttca acaagtcctt ctaaggttgt atctttataa tagttgtagg tagctgggaa
    28981 taaatatcct tccaattgat aaatagcttt tgatttatca ggtaaacttc ctagtaaatt
    29041 tggatattta gctaccattt ttttaataaa cg
//
LOCUS       DATRSD010000014        27224 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRSD010000014 DATRSD010000000
VERSION     DATRSD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27224
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.014"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..408)
                     /locus_tag="VBD85_000516"
     CDS             complement(<1..408)
                     /locus_tag="VBD85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO7133090.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPL"
     gene            complement(383..1222)
                     /locus_tag="VBD85_000517"
     CDS             complement(383..1222)
                     /locus_tag="VBD85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO7133091.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(1215..2051)
                     /locus_tag="VBD85_000518"
     CDS             complement(1215..2051)
                     /locus_tag="VBD85_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7133092.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGVEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFREKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(2164..3822)
                     /gene="recN"
                     /locus_tag="VBD85_000519"
     CDS             complement(2164..3822)
                     /gene="recN"
                     /locus_tag="VBD85_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO7133093.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGSRASVEVIRHGANKAEIEGFFSVEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(3834..4307)
                     /locus_tag="VBD85_000520"
     CDS             complement(3834..4307)
                     /locus_tag="VBD85_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO7133094.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(4294..5121)
                     /locus_tag="VBD85_000521"
     CDS             complement(4294..5121)
                     /locus_tag="VBD85_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEO7133095.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGMVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPIQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(5114..5986)
                     /locus_tag="VBD85_000522"
     CDS             complement(5114..5986)
                     /locus_tag="VBD85_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7133096.1"
                     /translation="MVTIEKIDEAIHRYYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(5986..6201)
                     /locus_tag="VBD85_000523"
     CDS             complement(5986..6201)
                     /locus_tag="VBD85_000523"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO7133097.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQRTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(6179..7519)
                     /locus_tag="VBD85_000524"
     CDS             complement(6179..7519)
                     /locus_tag="VBD85_000524"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO7133098.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLIPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTFSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRIHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(7645..8481)
                     /locus_tag="VBD85_000525"
     CDS             complement(7645..8481)
                     /locus_tag="VBD85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO7133099.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNQTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(8478..9332)
                     /locus_tag="VBD85_000526"
     CDS             complement(8478..9332)
                     /locus_tag="VBD85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO7133100.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(9471..11165)
                     /locus_tag="VBD85_000527"
     CDS             complement(9471..11165)
                     /locus_tag="VBD85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7133101.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADILLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQDPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(11452..12186)
                     /locus_tag="VBD85_000528"
     CDS             complement(11452..12186)
                     /locus_tag="VBD85_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7133102.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKEVFEQTKEIRTRDFLSKVL"
     gene            complement(12179..12871)
                     /locus_tag="VBD85_000529"
     CDS             complement(12179..12871)
                     /locus_tag="VBD85_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7133103.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
     gene            complement(13014..13244)
                     /locus_tag="VBD85_000530"
     CDS             complement(13014..13244)
                     /locus_tag="VBD85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO7133104.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13542..15803
                     /locus_tag="VBD85_000531"
     CDS             13542..15803
                     /locus_tag="VBD85_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7133105.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQSGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKVQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESAKG"
     gene            15989..16444
                     /locus_tag="VBD85_000532"
     CDS             15989..16444
                     /locus_tag="VBD85_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7133106.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            16508..16810
                     /locus_tag="VBD85_000533"
     CDS             16508..16810
                     /locus_tag="VBD85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO7133107.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTKAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(16927..19719)
                     /locus_tag="VBD85_000534"
     CDS             complement(16927..19719)
                     /locus_tag="VBD85_000534"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO7133108.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTKMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(20004..20774)
                     /locus_tag="VBD85_000535"
     CDS             complement(20004..20774)
                     /locus_tag="VBD85_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO7133109.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(20784..21572)
                     /locus_tag="VBD85_000536"
     CDS             complement(20784..21572)
                     /locus_tag="VBD85_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7133110.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(21574..21828)
                     /locus_tag="VBD85_000537"
     CDS             complement(21574..21828)
                     /locus_tag="VBD85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO7133111.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWFPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(21831..22436)
                     /locus_tag="VBD85_000538"
     CDS             complement(21831..22436)
                     /locus_tag="VBD85_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO7133112.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEIIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(22448..23122)
                     /locus_tag="VBD85_000539"
     CDS             complement(22448..23122)
                     /locus_tag="VBD85_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO7133113.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHVVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(23128..24408)
                     /gene="ftsZ"
                     /locus_tag="VBD85_000540"
     CDS             complement(23128..24408)
                     /gene="ftsZ"
                     /locus_tag="VBD85_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO7133114.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHTQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(24430..25803)
                     /gene="ftsA"
                     /locus_tag="VBD85_000541"
     CDS             complement(24430..25803)
                     /gene="ftsA"
                     /locus_tag="VBD85_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO7133115.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKANIGERIRG
                     IFGSMFD"
     gene            complement(26076..27212)
                     /locus_tag="VBD85_000542"
     CDS             complement(26076..27212)
                     /locus_tag="VBD85_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO7133116.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
BASE COUNT         8464 a         5460 c         4296 g         9004 t
ORIGIN      
        1 aagcggaaga gttcctactg aaaatgaggt cactttaagt tgaacagcgc catttgttaa
       61 acgataaggt tcaaaataga tgtataaagg cacttcataa cctaataatt gataagagcc
      121 ttcaaatagt atagaagatg aagcagcata aatcttataa ttcatcttct tagtttgata
      181 ttgttttaaa taaagtgcaa ttgtcttatt cagttgcgac ttattggtcg taaaagtacc
      241 tacttttact ttatcttgta ccccagtcga aatttttcct gtattaggct cacgtacttg
      301 aattaagcga cttgcaatca ctgctgtgaa ggaaaggtta attgccaaca ataagagaaa
      361 tgcccatttc cagaaattta aattacggcc agttttttct tgtttcattt attttctcca
      421 taacggcgtt agacatgatt tgatagccaa tattattggg atgaaaatgg tctccagtaa
      481 agagagcatc attagtgata cttgcctgac tatttgatga ctctgtaata ccctctttac
      541 catttattcc cttataaagg cggtcattaa ttgggacaaa ataaacattt tctgaagcat
      601 caactacttc ttttgtagct ttattccaat tatcaataac ggtttgcatt ttagttaatt
      661 gtggaaagtt taggtaaaaa ggattataaa tgcctaaaac ataaataggc aatttaggat
      721 tatcttgtct tgcttttgca aggatttctt tcaaacgttc cttatatgct tctgctggtt
      781 tctcaaagga atttagtgat aaatgactga gctctttacg aataacagcc aagacatcat
      841 taccaccaac agttagcgtc aataaatcag ctttctctaa atctttttcg atttgaggat
      901 ctgtcgtcat acgttttaaa atttgttgac tagtattccc agacacacca taattaacag
      961 aagtcacttg gtaagagtat cgattatgga gtgattctga tagcagtgga acaaaaccac
     1021 cttgagaggt tgtatcgccc acaccttcgg tcagagaatc tccaagagca acatagttaa
     1081 gtgggataac tttctttgtt aggaagtctt tttttgttaa tttaggattt gattttggaa
     1141 tgatcaagga aaaaatccca aaagataaaa gaagacttac cagaaaaaaa gaaagtccgg
     1201 taagtatttt tttcttattc ataacgaacc ataacagcaa atgcatcctc accagtatga
     1261 gtttggatga ttgagccagt ttctaaaacg gatataggag cacctagaac agctagtttt
     1321 tctctaaagt tattagccat atcagcttta ccacagtagg agatgccaat ttcagcaatt
     1381 tttcgtgttt gggcactttc aacaaaatta tctaaccatt tgctgaaggt ctttagaccg
     1441 cgacctttaa caatcggtac taattcatgg tttgttagct ccataattac ttttatatta
     1501 agcaatgaac ttagaagacc cgttacacgt ccaatacgac caccttttac taaattttca
     1561 agggtcgata cgccaataaa taattctgac ttctggcgta cttcttccac acgagccaag
     1621 atggtatcta aatcagctcc ctctttagct aatttcgcag cttctacaac ctggaatttt
     1681 tgacactggt ctgtaaaagt agaatcaata actgtaacat ctgcaccagc aatattagct
     1741 ccctggcgtg atgcttcaat agttcctgat agtgtatgcg ttaaatgaat tgcaatgata
     1801 tgttccacac cttcattcat taatttttca taaatttctg caaaaacacc tacaggtggt
     1861 tgacttgttt ttggcaattc cttacttcca cgcattaggt tcaagaattc cccttgtgct
     1921 tttaagtcat tgtctgaata caaggtacca tctatcataa ctgacaaggg aacaactgta
     1981 atgtcaagct ctttaataag ttctggttca atggtaatgg aagaatccgt tacaattttg
     2041 attttactca tatcctatcc tattctatac tttaatattt caataccctt attataacaa
     2101 aatttaagtg tatcaacatt aatttctcct aacaatgtta gctttttaaa gacgttacca
     2161 aagttaacta ccaagtaatt ctttagcttg agtacgtgca gtgtctgtta cattgtttcc
     2221 tgccaacatt ttagcgattt cttctacacg ttcttcatag cttaaaagcc ttaccgttga
     2281 aactgttgaa gagtcactgg aaattttttc aataaaatat tggtaatccg ctattgcaat
     2341 aacctgagct aaatgagaaa ttgctaaaac ttgtccatga ctaccaatct tgtgaatttt
     2401 ttgagcgata gcctgggcca cgcgaccaga aactcctgta tccacctcat caaatacaat
     2461 ggaagtttta tcttccttac gtgaaaaagc agatttaata gctaacatca atcgagataa
     2521 ctctcctcct gaagccactt ttaccaatgg cttaaagccc tctccgggat tcgttgaaat
     2581 ataaaattca acgatttcgt tcccttcttt attaaatttt ccttttgtaa attgaacttg
     2641 gaaatcagct ttctccatat aaagctctgt taattcttgt ttgatttcat tttccaattg
     2701 tttagctagc ttatggcgct ctaaacttaa ttgatttgct gattcaatca aatcgtgttc
     2761 taaaatcttt aattcttttt ctaaagcatc tgaggactct tcactacctg tcaatagact
     2821 atattcttta gtaatgttat caaaatagtc taaaacatca ttcacatcac cgccatattt
     2881 tcgcgttatt gtgttaataa catctagtct attttcaatt tcttgtagta aaccagcgtc
     2941 aaaatctaaa tcatcgataa cgtcacctaa acgtttagta acttcttcaa taacgtagta
     3001 agcttctgaa agattggtgg aaagatcttt atattctcga tcaaattctt ctaaagccat
     3061 aaggtcattc attgcagaac gaacattcga taaacttgaa aactcttcgt tatctaacat
     3121 aagatatgca tttgtcaaag tatctgcaat attcttatga ttcattaatt tatcacgttg
     3181 cttgagtagc gtttggtctt catctgattt aagggctaca gactcaattt ctgctatttg
     3241 aaattctagc atttcaatac gtgatttatt ctcttgttca tttttttgct tatccaatac
     3301 gcgtttacga agctgacgat aagcatcaaa aagactttga taacgttctt ttatgacatt
     3361 aaattctgta ttaccaaatt catctaacat cagaatgtgc atgttaggtt tcattagttc
     3421 ctcttggtca tgctgaccat gaatgtctac caaataatgc cccacagctt ttagtgttga
     3481 caaattcacc atttgcccat taattcgact aacgctgcgc ccattttgaa agatttctcg
     3541 gcgaataatt aattcatctg ctaattcaat gccattttct tccaataatt ggactaatga
     3601 ttgatttttt tcaacagaga aaaatccttc aatttctgct ttgttagcac catggcgaat
     3661 cacttcaacg ctagcacggg atcctaacat catattcata gcatcaataa tgatagattt
     3721 ccctgcacca gtctcaccag ttaaaacagt cattcctgtt tcaaaattaa gtgaaatttc
     3781 ttcgataata gcgaaattct taatagaaat ttctaaaagc ataaggtcct ctactaatct
     3841 tttttctcca acattgcaat cattgattga cgaatgtgat cagcatctgc ttctgatttt
     3901 gcaatcaaaa ggagactatt atcgtcagct gtcaaactaa aaatatgttc ttgacaatgt
     3961 gacattatga aggttttaat taattgactg ttacctggta tgacattaat attgataaat
     4021 tgctctaaac ctagtagctt atctgatatt gataaaatac ttgttttaat aggctttgcc
     4081 acagcagttg taaagatagg gtcgttttca tttgacaaac cgtaaatata gcgacctttt
     4141 gctgatggca cttttataat gccaatttca ttcatatcac gtgaaatagt tgcttgagtt
     4201 agagttaccc cataagcttc caatctgcgt aacaactcat gttgtgtttc aacagcatgg
     4261 ttaagaacaa tttgcttaat taaatttaaa cgttcactct tcttcatttt tcttaaattc
     4321 cttatgtgct ttttctataa catcttgtat ttggtcaagc acaagatttt gtggatcttg
     4381 acacttttgc aaatgcatta aaaactcaat atttccatgt ccaccttgaa tgggcgaaaa
     4441 atcaagatgt ttaaccgtat atccataatc tttcgtgaaa ttggtcactg ttgtcaaaac
     4501 cttttcatga accaacttgt ctttgacaat accattttta ccaatttgct cacgacctgc
     4561 ttcaaattgt ggtttaatta atgccactac ttgtccacca tccactaaaa tttcttttag
     4621 agctggtaaa atcaaattaa gagagataaa tgagacatct atcgatgcaa attcaggcag
     4681 tccctccttg aaatcttctt tttgggcata cctaaaatta tattgttcca tagaacgaac
     4741 acgatgatcc tgacgtaact tccaaactaa ttgatttgtt cctacatcta ctgcgtaaac
     4801 taaacgcgct cctgattgta gcataacatc agtaaaacca cccgtagagg cgccaatatc
     4861 tatagttagc ttatctgcaa ctgaaatttc aaaaacttgt aaagcttttt ctaatttcaa
     4921 tccacctcta ctaacatatt ttagtttttc accttttagt tttaattcag tatcgtctgc
     4981 aaccttttca cctggtttat cataacgttc tccattgata acgttaatca ccattcctgc
     5041 cataacacca cgtttcgctt gctctcgtgt atcaaaaagt ccctgtttat aggcaagaac
     5101 atctaccctc tctttagcca ttcaaccgta atccctctat tatatctatt atttttctag
     5161 cattaaagtt agcttttttc tctagatttt gaaaaattgc ctgagctttt ttcaaagtat
     5221 catcaagtat ttcctgtgac ttatccaaac ccaataaatt tggataagtt gtcttttctg
     5281 ctacaatgtc tttatttggt gtcttcccca attcttcaaa actagcagtc acatctaaaa
     5341 tatcatcacg tacttgaaaa gcatgaccga ttaaaaggcc agcttgatgc agttttcctt
     5401 ttacttcttc cgacttctca gctaaaatac ctgctgcaac aaagggataa gctaacaggc
     5461 gtcccgtttt attaatatga atcaagctta aatcgtcaat agataaaact ttgttttctc
     5521 ccttcatatc taacatttgt ccaccaacca tgccaaaact acccgcagac atagacaata
     5581 actctattaa cctaacagta acatcagctt taaaaccagc ttttacaact aaatcaaatg
     5641 ggtctaaaaa aagggaatct ccagctaaaa ccgctgtagc ttcatcaaac tttttatggt
     5701 ttgttaagcg gccacgacga aaatcatcat tatccatcgc cggtaaatca tcatggatta
     5761 aagagccggt gtgaatcatc tccaaagcag cagccacatc ataatgacca tctattagtt
     5821 caactccaaa tccctctaaa atttctaaaa ggatgcgtgg acgaatacgt ttcccacctc
     5881 catcaactga gtagaggata gccttaatca aatcaggaga aacgacacta tgtgtctgtt
     5941 tgtaataacg atgaatagct tcatcaattt tttcaatagt aaccatcaag tatccatttc
     6001 tacttccgtt ccgtcagctt gcatcacttt tacaagcgtc tcttcagctt cttttaaagt
     6061 tctttgtaac tcttttgaaa taagcattcc tttttgaaat tcagcaatgg catcttctaa
     6121 ggcaacatca cctgtttcta aacgtgagac aattgtctct aattcctgta aattttcttc
     6181 aaatgttttc ttgtctgaca tccttcacct caacttctaa aagaccatct tgcatcttaa
     6241 cttgcaaatg atctccttca tttattccat tagtagtaga tactaaagta tggttttttt
     6301 cgataatagc gtaaccacgc gctacaattc tagaactatc caatgatata agcgcatctt
     6361 gagccttttc aaaacgtgct agcttgctgt catactgact agtcattgta cttgataata
     6421 gtcgtctact ttgtacaaca cgctcttggt aagtatgaat acgctgtttt aaatctatgc
     6481 cttgcagttt ttgatgaagc aaaccttgct tttgtcttac tgtttgtagt ttatcacgca
     6541 tagaaaatgt taactgctga tttaggttat ctaatttttg aaggaaacca tcatatagac
     6601 gctctggttg cctaaaaata actgattgct ttgatttttg caacctctcc tcttttgtcc
     6661 tgataagacg aaggctagac tgatacattc tattttcacg ttcagtaatc caagacaaga
     6721 tatcaatttt agtaaccggt gtagcaagct cagctgctgc agtcggagtt gctgctctgc
     6781 gatcggctac aaaatctgct aaagttgtat cagtttcatg tccgacacta gaaataacgg
     6841 gaagacgaga ttcaaaaata gcttctacaa cacattcttc attaaatgcc cataagtcct
     6901 caatagaacc tcccccacgg ccaacaatta atagatccaa atcctttttc tcgttagcta
     6961 aagcaatagt ttgtgctatc tcctgagctg cgccctctcc ttgaactttt gtggggaaaa
     7021 gtaatatttc cactcctggg aatcgacgag atactgttgt aataatatcc cgaataacgg
     7081 ctccacttgg actagtcaca actcctattt ttctaacaaa ttgaggaatt aattgcttat
     7141 gacgatcatc aaaataacct gcttgagaga gtttcttctt tagttgttca aattggatag
     7201 caagagctcc tataccatcc ggttctgctt tttcaacaat aatagagtag gagccgcttg
     7261 gttcataaag ttgaacacgg ccaactacat taaccttcat cccttcctct aattcaaaac
     7321 ccagtttttt gaattgacct gaccacattg tcgcctgaat gactgattta tcatctttta
     7381 aagagaaata ttgatggttt gggcgacgac gaaaattaga cacttgccca gttaaataaa
     7441 ccctctctaa ataagggtct ttgtcaaatt ttaattttaa atacttagtc agtgtcgaca
     7501 cggataaata gtctgacata gcacctcctc atgagagttt tattaacttc attataccaa
     7561 aaaaacttaa aaaataccta tttgctctac aaatagctcg ataaagcaaa agaagtgtca
     7621 tgctaacacc tctctactta ttttttattt agatagtcta gccatttctt ttggaatctc
     7681 atcactaatc gttgtcggta gaacaacgta ggcttcctta gataattctt gagcaattgc
     7741 cgaatgtaga taaactccca ctgatacctt atctagagga gaggcttctt taaattgcgc
     7801 taacatacct gcaatcatac cacacaaagt atcccccatc cctccagtcg cctggtaagg
     7861 acctcctact ataatttctt tttcgtctaa atcttgaaaa atacgcgtat gcgaactctt
     7921 agcgactaag atcgttcctt tgggaaaaga cttaagagcg gtttgggtat tttcttttgt
     7981 ctgttgcgat acagcaatac ctgacaatcg ctcccattct ttttgatgag gtgtcaacac
     8041 taaattttta gcctttgttt gcggaaacgc accttctgat aacagagtca aggctgatcc
     8101 atcaatgatg acactttgat gtgattgaat cttctccatg gtctggttca agatttttcg
     8161 agatgattct gatactccta atccaggacc aattacaata acatcacttt gaacaattga
     8221 tttttccaac catttgtaat catcactatc aaacgccatt acctctggca gttgactatg
     8281 caaagagggg atattgcaac tttgggttgc tacagtaact aatcctgcac cagttttgac
     8341 acaggccaaa gctgccatta taatagcacc tccataggga taaaaacctc ctatcagaag
     8401 aacactacca taatcacctt tatggcttga tttagggcgt ggcttaatta acgccctcgc
     8461 ttcttgttca ccaacaatca tagactcaca cttctaagag ctgcttgata agtttgttcc
     8521 aaaagcattg taatcgtcat aggtcctacg cccccaggaa caggtgttat catactagca
     8581 acttctgcca cttgttcaaa tacaacgtct ccaatcaatt taccattttc atcgcgattc
     8641 ataccaacat caatcaccac agcaccttct ttaacgaagt cttttgtaac aaagtgcccc
     8701 tgaccaatcg caacaataag gatatcagcc tcctttgtta cctcagaaag gtttcgagtt
     8761 cttgaatgtg tcaaagtaac tgttgcgttt ttatcaagga gaagttgtgc cattggctta
     8821 ccaacaatat tggaccttcc gatgataaca gcatgttttc cctctaaatc cacatggtac
     8881 tctctaaaca tctccataat accagctggc gtacaaggta ccatcatcgg tcgtcctgac
     8941 caaagatgac ccgtattcat tgggtggaaa ccatcaacat ctttcttagg atctattgct
     9001 aaaataattt ttttatcgtt aatatgttgt ggtaacggaa gttgaactaa aataccatgg
     9061 atacttttat cttcattgta ttgatggata atatcaatta gttcttcttg agaaatggat
     9121 tcagataatc gtagagtctc acttttaaaa ccagcttcta atgcagagcg ttctttattt
     9181 cgaacgtaca cttggctagc tggattatcc ccaacaagga ttacagctaa accaggtatg
     9241 ataccatgct gttcctttaa cctttcaact tttcgcccta actcagcctg cattttttgt
     9301 gataaggctt taccatctat taattcagtc attgatactc cttcttcatc cgttttttct
     9361 atattatatc aaaaaatagc gattatgcta ttttatatcg ctattcattt tgtatcaatt
     9421 ttaatcaata gaaaaggcaa tgaatccctc atcgcctttt tacatatgac ttatttagta
     9481 ctattcattt tagcacgaca agccgactca attgcattaa gtttcgataa actatctgct
     9541 tctgtacaac caatcgtata aaggtaacat tttatcttag gttccgtccc tgacggcctt
     9601 aaagcatacc atgaaccctc attaaaataa tattttaaac aattttgctt tggaaaatcc
     9661 ttataaccat ccttgaaatc aatagaattc tccaatgtca tctcacctac ttgtaatatt
     9721 gggtcctgtc taaaatcctc cataatacga ctaatacgtt cttgcccctc agcaccctct
     9781 aactcaagag aaaattggcg ctcgttgtaa tagccaaatt catcgtaaat ggtttgcaaa
     9841 acgtctaaaa gtgtttgccc tcgttcttta taataggcag tcatttctac taccatcatt
     9901 gaagcactca cagcatcttt atcacgtaca aaagtgccat agcaaaaacc aatactttct
     9961 tcatagccaa agagataagt tttatccttt gagatatcat attcattagc ttttccacaa
    10021 atatttttaa atccggttaa agtttcaaca gtttcaatat tatatttatc tgcaataact
    10081 tttgatagat caccagttac aatggatttt accaatacag gatgatgtgg caaattccct
    10141 aaggcacatc gttgtgaaaa aatataatag gaaagaagtg cccctatctt attaccattt
    10201 aagaaaatat attctccttt actatctttg acttccaatg ctactcggtc acaatctgga
    10261 tctgtggcaa gtaagatatc tgcatcaact gactttccta gagattctga ataggcaaat
    10321 gctttaggaa cttcagggtt tggatagcca accgttgtaa aatcaggatc gggcatttcc
    10381 tgctcaggta ccacataaac attttcaaaa ccacggcgtc ttaaaacttc gcgcacaggt
    10441 aaatttccta cgccatttaa aggagtataa actactcgga ctgacttatc aatgttagta
    10501 tcattaatag ttaaaccaag cacttctttt ttatatgctt cttcaatact ctcatcaata
    10561 taacttgcca aaccacttgc cagagcctct tcaaagggta tttgcttaat ttgctgataa
    10621 tcggttatag catccatatg attggcaatt tggtcagcaa tatcatctaa aatctgagat
    10681 ccttctttcc aatatgcctt ataaccatta taagcttgag gattatgact agcagtaacc
    10741 atcacacccg aaacacatcc tagagcacga atagcatatg agcacattgg tgttgggcga
    10801 atccctttat aaatataaga cttaatacca tttgctgcca taatggaaca agttaattct
    10861 gcaaattcct tagattgata acggacatca taactaactg caattccacg tgcaatagct
    10921 tcagggccat gatcaataat cgtattagct aatgcttgtg ctgctttccc caccatataa
    10981 gtattcatac gattggttcc tgctccaagt ttacctctca atcccgccgt tccaaattct
    11041 aatgttttgt aaaaacgatc ctgaatttca gattcatcgc ctttaatagc ttctaaatct
    11101 gacttaatat ctttaccgag tgagtcgttt tctaaccact cttgataaat ctctttataa
    11161 ttcatatgag acatcctatt ctaagaccaa aatggtaaaa atcagctcca tgataagaga
    11221 ttttagttct tcaggagcta actgaatagt atcaaattaa ccttataaag tcaacttatt
    11281 ttataagtta actgcaccta atatatttaa gacatctaaa tttttttcat catcctatca
    11341 ctcttaatct acattatata tagacttacc aacttccaaa caatacagta acaatttact
    11401 cataacaatg gaataacaaa agtctaaaac aagttgtttt agacaatgaa attataatac
    11461 tttacttaag aaatctcttg tgcggatttc ttttgtctgc tcaaatactt ccttaggggt
    11521 cccttgctca acaataattc ccgcatccat aaaaatgaca cgatccgcta cttcacgtgc
    11581 aaaacccatt tcatgagtga caataaccat cgtcatacca gatttagcta aatcttgcat
    11641 aacagtcaag acttcaccta ccatttcagg atcaagagct gaagtaggtt catcaaaaag
    11701 aaggacatca ggattcattg caagacctct tgcaatagca attcgttgtt gttgtcctcc
    11761 agataagcta gctggataag cattagcctt ctctttgagt ccaacttttt caagtagctc
    11821 gtatgctttt gtctgagcat caagcttaga aagtcccttt gtcttaatag gtgataaagt
    11881 aatattttct agtacagtca tattgggaaa tagattgaac tgttgaaaaa ccatgcccat
    11941 tttttcgcgc attttaaaaa tatcattctt tttgtctgtt atatcaatcc cttcaaaagt
    12001 cactgttccc tttgttggta cttccaagag attcattgtt cttaaaaatg ttgacttacc
    12061 agagccagaa gggccaataa taaccaccac ttctccttga tgaatatcca agtcaatgcc
    12121 ttttaaaacc tcatttttcc caaaactttt atgaagccct tgtattgaaa taattggctc
    12181 aaccatctat ttttacccct ttcccaagat atttctccat ttgttttaac aaagctgaga
    12241 gaatcgttgt caacattaaa tagtaaaatg ctgcaaataa taacggtgca actggtgagt
    12301 aagtagccgt tacaacggat tgtgctccgt tccataattc catgacacca atagtttgaa
    12361 ggagggcact atccttaata attgtaataa attcattccc tagagcaggt aaaatatttt
    12421 taaaagcttg gggtaagata acatagcgaa gtgtattttt aggtcgaatc cccaacgagt
    12481 aagctgcttc tatttgtcca gatggtacag cctcaatccc tgcacgtaca atttccgaaa
    12541 tataggcacc actatttaag gaaatgataa tgataccagg taaaagtcgt gtaaaatcta
    12601 aatctaaaac accaaagcta atcgttggta aattgttaaa atgcatccat gcgaaagcaa
    12661 tcataatttg aactaccatc ggtgtcccac gaaataccca tacatagaaa ttagctaata
    12721 ttgtgagaaa atgtaaatta gtacgcttta ctaaagcaat taacacgcct ataatagttc
    12781 caaaaaaaac aacacatgtt gaaatcataa tggttaccat cacaccataa ttaaaatagg
    12841 accaatattg tggtaaaaat gaaaaattca ttgacatctc ctaaaaaagt aattgccaat
    12901 agtctatcac acaaagaata aaaatacaag tttaaataaa tatttttgtt tttattctat
    12961 tttactgtat atttagatat aaaaaaaaag accttcaagg tcttttataa ttatcagtat
    13021 aataaatcgt aaatttccat tgcgataagg tcaatgctgt caaatgaata tgattcacgc
    13081 gcttctacat cacgtaatgt gaaaacagga ccgttttcag ggtcattact aaaagatact
    13141 tctgctacaa caacaccttc acgttcaaaa ttacgtttta aattaccacc atcagtcgcc
    13201 attaattcga ggcgattgat gattctcact aaatgtgatt ccatcttact tactcctata
    13261 tattttctag tcctttctat tttaacacaa aagtgaccaa agcaacatga aaagcaacat
    13321 ttttactaac tttttaacga ttttatcgtc taaaatccgc aaataacgat ttttctcagt
    13381 agtcatctat ttttcaattt agcactctaa tatattcagt gctaaaaaca gaactttttt
    13441 cttgatttct aggctttagg tgctataata aaagcataaa gtttgactat atttgactat
    13501 atacacatca atagttaaat gtcataagaa aggggtagcc tatgctctgt caaaactgca
    13561 aactaaacga atcaactatt catttgtata caaatgttaa tggaaaacaa aagcaagttg
    13621 atctttgtca aaattgctac caaattatta aaactgaccc taataatcca cttttttcag
    13681 gtttaaatca tgtttcacat gcgcctggtg gcatcaatcc tttctttgat gatttctttg
    13741 gagatttaaa taatttcaga gcctttaatg gccaagattt accaaacact cctcctacac
    13801 aatcaggagg aaaccgaggt ggaggaaacg gtaatggacg aaataataat cgcaaccaaa
    13861 ctgcaactcc tcctcaagcc aaagggattc tagaagaatt tggtatcaat gtcactgaaa
    13921 tagcccgcca tggtgatatt gaccctgtta ttggacgtga ctcagaaatt attcgtgtta
    13981 tcgaaatcct taatcgtcgt acaaaaaata atcctgtact tattggtgaa cccggcgtag
    14041 ggaaaactgc tgttgttgaa ggacttgctc agaaaattgt tgatggtaat gtgcctcata
    14101 aacttcaagg caaacaagtt attcgtttgg atgttgtgag tctagtacaa ggaacgggca
    14161 ttcgtggtca atttgaagaa cgcatgcaaa agttgatgga agaaattcgt caacgtcaag
    14221 atgttattct attcatagat gaaattcacg aaatagttgg agctggtaca gctggcgaag
    14281 gtagtatgga tgcaggtaat attttaaaac ctgcccttgc acgtggagaa ttacaactag
    14341 taggtgctac tactctcaat gaatatcgta ttatcgaaaa ggacgctgcc ttagaacgcc
    14401 gtatgcaacc tgtaaaagtc gatgagcctt ctgttgaaga aaccattact attttgaaag
    14461 gtatccaaaa aaaatacgaa gattatcatc acgtaaaata taataatgat gccatagaag
    14521 cagctgcagt actatctaat cgttatatcc aagaccgctt tttacctgat aaagcaatag
    14581 acttattaga tgaagctggt tctaaaatga acctaacact aaattttgtt gatccaaaag
    14641 aaattgatca acgtctcatt gaagcagaaa atttaaaagt gcaagcgact cgtgaagaag
    14701 attacgaacg tgcagcttac ttccgtgacc agattgcaaa atataaagaa atgcaacaac
    14761 aaaaggtcga cgatcaagat acacctatta ttaccgaaaa aacaattgag cacatcattg
    14821 aagaaaaaac gaatatccct gttggtgatt taaaagaaaa agaacaatct caattaatta
    14881 atctcgcaga tgacttgaaa caacatgtga tcggccagga tgacgctgtc attaagattg
    14941 caaaagctat tcgtcgtaat cgtgttggtc ttggtagccc aaaccgtcct attggttcct
    15001 ttttatttgt aggaccaacc ggtgttggta aaactgaact ttctaaacaa ctagcaattg
    15061 agctctttgg ttcagctgat agtatgattc gttttgatat gtcagagtac atggaaaagc
    15121 atgctgttgc taaattagtc ggagcgcctc caggatacgt gggatacgag gaagctggac
    15181 aattaactga aaaggttcgt cgaaatcctt actcgctcat ccttctagat gaaattgaaa
    15241 aagctcatcc tgatgtcatg catatgttct tgcaggtcct tgatgacggt cgattaacag
    15301 atggacaagg aagaactgtt agttttaaag ataccattat catcatgacc tcaaatgctg
    15361 gttctggtaa aactgaagca agcgttggct ttggtgcctc acgagaaggt aggacgaatt
    15421 ccgtactagg tcaactaggt aacttcttca gccctgaatt tatgaaccgc tttgacggta
    15481 ttattgaatt caaggcttta gataaagaga atctcctcaa tatcgttgat attatgttat
    15541 ctgacgttaa tgcacgtctc gccatcaatg gtattcatct agatgtcact gataaagtga
    15601 aagaaaaatt ggttgattta ggttacgatc caaaaatggg agcacgtcca ttacgtcgta
    15661 ccattcaaga acatattgaa gatgctatca cagattacta tcttgagaat ccaagcgaaa
    15721 aagaacttcg tgctattatg actagcaatg gaaatatcat aataaaatct tctaaaaaaa
    15781 ctgaggaaag tgcaaaaggt tgagtaagaa actcaacctt ttttcacact cttctcaaaa
    15841 aataatagcc tctataaagc tttttctctc ctatctcgtc tttaaatttt aatacaatta
    15901 ttaaggaaat attccaaaaa ttttagtata ataaataaaa aataattaaa gcttttaaaa
    15961 tcataaaaca caatcaggag agagaactat gactaatcct actttcggtg aaaaaataga
    16021 taatgtagac tatagatcac gatttggtgt ctatgctatt attccaaacc caactcatga
    16081 taaaattatt ttagtacagg cacctaatgg tgcttggttt cttccaggtg gggaaattga
    16141 agaaaatgaa aatcacctag aagctttaac ccgtgaatta attgaagaat taggttattc
    16201 tgcaacaatt ggccattact atggtcaagc tgatgaatat ttttactcta gacaccgtga
    16261 tacttactac tataatcccg cctatattta tgaagttact gcctatcata aggagcaagc
    16321 gccactagaa gatttcaacc atctagcctg gtttcctatc caagaagcta aagaaaagct
    16381 aaaacgagga agccatagat ggggtgtcca agcttgggaa aaaaatcacc attctaggaa
    16441 ataattcata cctttcttcc taaatagtgt tataataaac ctaataagaa tcagggggaa
    16501 aatagttatg agactaatta atacaaccag tagtcatcct gaactagttc gaaatcaact
    16561 ccaaaataca gatgcaaaat tagtggaagt ctattcagct ggcaacaccg atgtcgtttt
    16621 tacaaaagca cctaaacatt atgaattatt aatttcaaac aaatatcgtg ctatcaaaga
    16681 tgaagaatta gaagctattc gtgagttttt cttaaagcgt aaaatagatc aatctattat
    16741 tattcaagag cagatgaaat cactccatac tgccaagtta attgaaattt catatccaac
    16801 aacagcataa ttaaaagaag cctaatcaag gcttcttttt tgatgactac taaacaaatg
    16861 tcttaccaac ctcttccatt aaaactggca agatatttgt caaaatatag cgttaaataa
    16921 tttttattaa gcttcaaaac cttgagctac tgcttcagga taatattgct caataattgc
    16981 tgcactagca tcacaaacag ctacaccata cgaacgcatc tttgtggttg gatcaatacg
    17041 gcgtgagcgt tcgcagactt caccttcagc gtgttcaaca gtaaatgcaa caccttcaaa
    17101 tgatactgag tcagctggtt tgtctgcttc atcagcaatt gttaattgtg agacaatcat
    17161 caagagtgca atatcgctat ttaacgctgt taataacgtt ttcacttctt gactagcata
    17221 aattgttaaa tgtgcttcta gagatttacc aataacttta gcgttacgtg cttcttcgag
    17281 agctttttga gcttgtgttc gtaacgtcat gaatgctgac cactcttcaa gaatttcttc
    17341 ttgaccagag aatgtctgtg caacaggcat ttctgctaat tgaacaaact cctcctcctc
    17401 atgttcaagg tatgaccaaa tttcttcagc agtatgcggt agaatcggag tcaaaagttt
    17461 tgtaagttta actaaaatat catagaaaac agtttgcata cgacgtcgtt ctggactgtt
    17521 agccgcttca atgtaaacca catcttttgc aaaatcaagg taaaatgctg atagatcaac
    17581 tgttacgaag ttaacgacag ctttataaat tgccataaaa tcataagcag cataagcttt
    17641 gttgattgta tccacaactt gattaaattt aatagtcata taacgatcaa cggcacctaa
    17701 attttcatag gctactgcat cttgtttagg attaaaatcg gaagtatttg caatcaagaa
    17761 acgcaatgta tttctgatct tacgataagt ttctgatgtt tgttttaaga tatccattga
    17821 aatacgaaca tcattacttg agtcaacaga agttacccac agacgcaaga tttccgcacc
    17881 aaattgtttt tcaacatcac tcggaagaat tgtatttcct agagatttag acattttctc
    17941 acctttacca tcaagtacaa aaccttgaga taagactgct ttgtatggag cgtgaccatt
    18001 aacagcaact gatgtaatta gtgatgagtt gaaccaacca cggtattgat cagaaccttc
    18061 aagataaaga tctgctggat aggaaaggtt ttcacgagca ttcattactc cattccatga
    18121 tgaaccagaa tcaaaccaaa catccatgat gtctgtttct ttctcaaaga gaccattagg
    18181 tgaacctgga tgagtataac ctgctggtag aaggtcctta gcatcacgtt gccaccaaac
    18241 gattgagccg tattcagcaa ataaatctgc aacgtgatca gtcacttctt ttgtcatgat
    18301 agctgtacca tcttctgcat aaaaaattgg aagtggaaca ccccaagcac gttgacgtga
    18361 gattacccag tcgccacggt cacggatcat gttataaaga cgttttttac cccactcagg
    18421 ttggaaattt gtcttttcaa tttcatcgag gatttcttga cggaatttcg aaacagaggc
    18481 aaaccattgt ggaaccgcac gccaaatgat tggctttttc gtacgccagt caaatggata
    18541 tgagtggttg ataacttcgc tggctaaaag aagatctccc aatttttctt ttacaagtgg
    18601 tgtcactttg tcatagaatt gaccttcaaa atcaggacca gcattttcca tcatcaaacc
    18661 acgactatct acggtaacta caacatcaag tccattagca ataccaacgt tatagtcgtc
    18721 ttcaccaaaa ccaggagccg tgtggacaat acctgtacca gaatctgttg taacatggtc
    18781 tccaaggata accaactctt ctacttctgt atcccatgga tgttctgtaa caatgtgatt
    18841 aagttcttta ccagtgtgat gagtcacaat ttcaaagttt tcccagccaa acttagcagc
    18901 gagactatct acaagaacct ctgcaagaag gtatttacgc tcactaccta ctggtgcaac
    18961 tacaacatac tccatatctg gtcctactgt taaaccgcgt gaagctgtta cagtaaatgg
    19021 tgttgtcgtc caaacgacga tataggtatc tgtatcaaga attcccttac catctttaac
    19081 tttattggca tagtagagtg atgtcgaatc aatatcatga tattcgattt cagcctcagc
    19141 aagggcagac tctgatgacc atgaccaata cactggttta gcaccacgat agatataccc
    19201 tttatctgcc atagcaccga aaacacgtac ttgatctgct tcataatctg gtgtcaatgt
    19261 aatataggga ttttcccaat cggcagaaac gcccagacgt ttaaaatcat cacgttgttt
    19321 gtcaacttgg ctgagagcat aatcacgaca catttcaagg tattcagcca agtccatttc
    19381 tttacgttta accccttttt tagctaatac ttgctcaatt gggagtccat gagtatccca
    19441 accaggcaca taaggagctc gaaaacccga cattgatttt gaacgtacaa taatgtcttt
    19501 agaaatttta tttagtgcat gtcctacatg aatattccca ttagcatagg gaggtccgtc
    19561 atgaaggtga aaggctggtt ttccttcatt caatgcttga cgttttttat aaatgtcagc
    19621 ttgatcccat gcttcttgcc attgaggttc cttatttgga agccctgcac gcattggaaa
    19681 agctgtttgt cctaaattca aggtttcttt taatttcata ctaactcctt atctcatagt
    19741 aatagttatc acaattttta aaattgctaa ataaaaaagc tttcatcaca taaaggacga
    19801 aagctcgtgg taccacctta attcagagca gaaaatagct atttcttact ccctcaacaa
    19861 cccgtaaggt gggttaagcc ttctacctta cttaaataat agttcaggta gaatgattga
    19921 aaatgataat ttcaatatta gggattgtag ggctctcacc atctcctact cgctgataat
    19981 ataatattaa aaccttgttt ttcttaatct tcaatattta atttaaaagt ttgagtttca
    20041 cctaaattga tcggttcttc catttggaaa ttattagtat ctaataaacc tgactcttca
    20101 agttgtttgt tactttcttc aacacgacgc tgtaattctt ccatttcatc tggtgagaat
    20161 tgacgagtag catcaaatga ctcagtgtca tccaccactg gtaaagcatc atcttcatca
    20221 agaacttttt caacaacttc tttgaaagaa gcatcagaat tttgaagata aatagctgtt
    20281 ggtttcaaca gttcttccca agcagatgag ttagctaatt ttaactgacc ttctaattca
    20341 gaaagtaagc gttggtggaa aacacgtgat tgacgtttca aatcttctgt ttctatagcg
    20401 acacgcttag catcatctgt cgcatcacga aggatttgat ttgcttttaa tttagcctca
    20461 tcaattaaat gttgagcatc aaatgtagct ttccccatta ggttagatgc ttcatcctgt
    20521 gctgaaattt tcacacgttc agcagtttct tgagctaaaa taactgattg acttaacgat
    20581 tccttcattt cgttgaagta agcgattttc tcttctaaat ctttaatgta ttgctcttgc
    20641 tcacgattac gtctaatcaa gtcttcgtaa tcgtcaacaa caatctctaa aaattcatta
    20701 acttcttctt cgctataacc gcgaaatttt gatgaaaatg ttttatcttt aatttcaagt
    20761 gctgtaagtg gcatgttttc tccttagtta tgtatcattt tgtctacttc taacttatat
    20821 ttttgatttt tagttaaccc taggttatgg ttcaacgtaa atcgaccata tcctctaaca
    20881 ctaatcaaat ccccctctac taattgttca gaaactcgat tgacgattgc ataattgacc
    20941 ttgactttat ctgcctcaat caatttcgtc gactgtgttc gtgaaatctt taaaatcgtt
    21001 gctaaaattt tatcaagacg taaactggaa gcaataacag ttagtttttg aatatcttgt
    21061 ttgggagtta gaagtttgga caagggaact tcagctaatt gtacactagc agttccaatt
    21121 tttgtaactg aatgaacaag gtgatttgtc atttgactat cgaccaaaac ctgagcacag
    21181 ccttcttcaa ctaggatatc ccctaaaatt gatcgtttta cacctaagta attcagtaaa
    21241 gttcccataa ttttagcatg ggtaaggtga ttaaatttag cattatactt tatttcaatt
    21301 aggctaaggt taaaatcaac catatctaac tgatagtatt ccggtgctat tattacttta
    21361 acatattctg tttgaaaata ctgaccagaa gtgtaataat gactacctcg cctctctaga
    21421 acactttcga gtattttaaa ttccctagga tttagaaatt cagtggtaat aaacgaatag
    21481 gtgttttcga catattgagc taaatggtct attttattga taaacgcata ctcttctggt
    21541 cgaaaatgct gataaatatc atctaacgtc atcttatgta aaaagcaaga ttaaaaatct
    21601 actcaacata tttagtaaaa agaatgctac tattactgta aaatctagac ctaaaaattg
    21661 tagattaaat cttctgagtg ggcgaataat cggatcaaca atctgtacca ctagtctacc
    21721 aatccatgaa tcgtatgctc ccggaaacca agataacaag gcataggcta gtaagatata
    21781 agaatagacc ctaattgttt ttaataatat taatagtact aatatcatat ttatcgtctc
    21841 ttcatatcaa aatcaaaact cgtctcttga ccagtctttg gaatattcat ctcctcaata
    21901 tcgaccataa cattggctgg tgttaataga aacattgaac tcccaacctt ttgtaatgaa
    21961 ccatataata ctctacttgc accatcaata taatccaagc aacgtcgtgc ttgcgcatca
    22021 agcatatatt gaaaatcaat caagacacat tcattaacga taagtaaatc aacaatttct
    22081 tgtgcatcct catacttacg gggatacttc agagcaattg ttacttgttc tctctgttgt
    22141 cgaactgagt ctttattttg aatcctagga gtagcttgct gataagcatt gtctctctgt
    22201 accctttgtc tctcctgacg attaggatta taggtagaag ttctcggacg actacctata
    22261 atctcttcct ctgcggagtt agtcatatga ctacgctgag aagcttcctg tgctgtagct
    22321 gctctggaat ctctttgcac tgaagttctc tcttgtactt cgtgtacttc attttcactt
    22381 acatcgtcag tgtcaaaata agaaataatt ttgtcaaatc tatcttttaa tgccatattc
    22441 ccctccatta tttaaagaat gaagttccta tccttacaaa tgttgatcca ttttgaatag
    22501 caatatcata atctctactc atgcccatgc ttaattctgt aaacggcatt cgttctatgt
    22561 tcctagcttg aagccttttc ctaagactct cagtctcttc aaaaattgat gatatttcct
    22621 ctttagttgc ttttaaggga gccatagtca tgatgccgat aagttcaatt ttatcgtacc
    22681 gagaaattaa gttcagtgca tcatctattt gctcaatagt aaaaccatgc ttactgtctt
    22741 ctcgtgatat attaacttgt agaaaacatt taactggttt ttgagcacgt ttttgaatct
    22801 cagctgctaa cttaacagaa tctaaagcat gaaaataatc aacataatta atgacatctt
    22861 taacttttcg acgttgtaaa ctaccaatga gatgccatgt aagcttttca tcttttaatg
    22921 cttgatattt ttcaagaaat ttatcaacac gattttcacc gatatgatta acacctgttc
    22981 taataagcgc ttctgttgtt tgacagttaa catattttgt tacagctacg acatgaactg
    23041 attcaggagc gcgacctgct tttagtgcca atttactaac attgtcaaaa atcgctgttt
    23101 tattttcttg taaattcatc attttaatta acggttttta aagaatggag gtgtttcaag
    23161 ttcatcatcc atatcatcat tttctgaaaa cgttgacata gataacttgc tatcaagttc
    23221 accttctgtc ggacgtgaaa tattatcacg acgaaggtcc caattaccaa aagcagaact
    23281 ttgttgctga ttttgtgtat gaggctgatt ttgttgagtt ggcatttcgc gagactctgt
    23341 catatcaaag ttgccacgac gatcaaagtt tgaatttgaa gtactttgac gttctgaagg
    23401 tgcttgatta gttggagctg atgttgtaaa accagatact tgattagttt tatctttacg
    23461 cacacctgtt gcaacaactg ttacgcggat ttcgtctttc atatccatgt caattgaagt
    23521 gcctaaccaa atattgacac ctttacctgc tgcttggcta acaatctctg atgcttcctc
    23581 tgcttcagta agtgtcatat ccataccgcc ggtaacattg acaataacgt cttctgctcc
    23641 atcaatcgtt gtttcaagaa gtggtgaata aattgctttt cgagcagctt cagtaatacg
    23701 ttcctcacca gaaccgatac caattcccat cagtgcatta cctttatttg ccataactgt
    23761 tttaacatct gcaaagtcaa ggttaattaa acctgggtta gtaatcaaat ctgtaatacc
    23821 ttgaacacct tgacgtaaaa cattatctgc ttcactaaga gcttcaagca atggtgtctt
    23881 cttatctaca atttcaagaa gattattatt tgaaatgata agcagtgtat caacttgttc
    23941 tcttaattct tgaatacctt cgatagcgaa gttagaacgt ttattaccct caaagccaaa
    24001 aggacgtgtg ataaccgcta ctgtaagtgc tcctaaactc tttgcaatac gtgcaataac
    24061 tggagccgca cctgtaccag atccgccccc catacctgct gtgataaata ccatatcagc
    24121 gccagtaagt gcttctgtta aaacttcttc actttcttca gctgctttac gaccaacttc
    24181 aggttgtcct cctgcaccaa gtccgcgagt taattttgga ccaagttgaa tgacagtttc
    24241 agcttttgag ctacttaagg cttgaatatc agtatttgct gcaataaact caacgccagc
    24301 gacaccttcg tcaatcatgc ggttaatagc gttaccgcca ccgcctccaa caccaataac
    24361 tttaataact gcaccttgta ctgatgctgt atcaaatgaa aataccattt ttattcctca
    24421 cttattgttt tagtcaaaca tacttccaaa aattcctctg atgcgctcac caatattagc
    24481 tttaggctca gtaggctctt gagcgttttc tcttgctcgg attggttcta aattagaatc
    24541 ttccatagaa gaggttaacg gctcagaata aggcattgtc gacattgtat ttgttttttc
    24601 tttataatca aagtcgactg gtttatgtct tagcatttca tcgccagtta cagcatgttg
    24661 tgcgataatg tcaacttctg acatcattcc tacataatca acgatactaa taacattagc
    24721 gaacattgga ttgcgaatac ctacttggtt tggaacgtgc aatttaactc tagttccaaa
    24781 aatttgttga gcaacctcga caactcctgg cataattgca ccacctccaa caagaataat
    24841 accaccaggt aaatcaagta aacgacctct acccaagtct tgtttgacat gttctaaaat
    24901 ttgacgaata cgtcctgaaa taatttgcga aagatagctt tctgtaattt caacaggttc
    24961 ctcgttacca acaacattaa cttgaactgt atcagaagta cttgcttctt caacattagc
    25021 ttgtccaaag ttgaatttca atgcttcggc gatttctact gttgtacgta acactttaga
    25081 aatatctttg gtgacataat cgctaccttc agaataaata ttagtatatt gcaattcttg
    25141 attacgcata gaagcgactg ttgtttgtcc gcctcccata tcaatcacag ttgcaccaaa
    25201 ctcacgttca ccttcgttta gaactgattt tgctaaggct agaggggcaa tcacaacatg
    25261 ctctactttt atacctgctc gttcaacagt tttacgaaga ttatgaagga tagttgttgg
    25321 tccagtatag ataagaccac gcatctcaag gcgaatcccc atcatccctc tagggtctct
    25381 aatcccttgg aaaccatcta caatgaattc aagcggaatc aatgaaataa cttctctttc
    25441 aggagtaatg cttttagtca atgctgattt aacaacactt tcgacatctt catccttaat
    25501 ttcttttgat tcattgggaa caggaatcat tccttgagtt ggttcaattt ggagaagatt
    25561 tgctggtaat ccaacattga ttttgtcaat ggtgatgcct gctttctcct cagcttgttt
    25621 taccgcctct ttgattgcag ttgctgctgc ctcaatatca ataattatac catcttttac
    25681 accggaacta gggacgttgc taaccccaat tacattcatt tcatttgcaa taaattctgc
    25741 aactaaaact ttaatcgagc ttgttcctat atccaaacca gtaaaaaagc catttctagc
    25801 cattcactcg acctcgctat ctttccattc ttttctattc tacatctgcc aaaaacagaa
    25861 ttattcagaa aatattatac cataaaaaaa gcagaattgt taccaaatta cgttttttta
    25921 ttaagcaaca tcaaattgaa atatttctaa cttatttaaa ccattcgcaa cttataacaa
    25981 acttaaattt ttttcaagaa atcaattttt actagcaatc tatgactgac ttgatatgta
    26041 taaaaaagac tgataaatcc agtctttttt tatcgctaat taacattttg agggttgggt
    26101 gcctgctctg ttgctatctg ttgtccttgt tgattagtat ttgagttatt tgtttgtcct
    26161 tgactatttt ccgcaacctg accattttgt gtttgtgttt tatcagttga tttatttttt
    26221 gtatcttccg ctttcacagg ggttgattca atagtacttg ttgttgtgta aactcccact
    26281 tccatatcaa caatagaagg ttccttaagg ttcttcttaa tttgtttgta aaaaggaagt
    26341 ctttctttaa atttagataa tggtattcta atactatttc catcatgcat atctaacagc
    26401 aggaggtcag gtgtcgtttt agaatcagct aaacttatca cctgaatctc acttattaaa
    26461 tcagggtcta aagcctttaa atctttaatt aatagcttaa tactatcttc cttatcaagg
    26521 ttaattgtta agaagtgctt tggtagctct gaactattta caggatcagc cttttttcca
    26581 gtttccaaga caggctgata tccttgcttt gtatgtgcat atgcaataat cttattttct
    26641 tgaacttgaa tatgaaactt attgggaaat tgataagtca tctgagctgt ttttacccat
    26701 acatcttctg cagctaaacg ttgttcaata gctttatgtt taaaaattaa agaaaagaaa
    26761 taatcgtttt tttgaatatt cgttttttct atcaaaatat catcaggtgt atgctgattt
    26821 ccactaactg ttattgtttt ttgcttacta aaaggagtta gtaggaaaac ggaaactaaa
    26881 atgactagga atgctactac aaatataggt gcagttctaa tgcgattagt tttggctaag
    26941 ctgttgacta ttttttcttt tttctgtttc ttttcaatct taggttttga aatctttgga
    27001 aaatgaagct tcttaatttt agtagtattt tgaggttctt caggagaaga aatatttaat
    27061 ttacttcttt tatctaagcg taatttttcg ttaatacgtt tttgttcttc ttcatcttct
    27121 ttgcgttttt ttaaaaattc aaggttacgc ttttgccatt ccgttaagac aacttcttct
    27181 ttttctgggg tatctgattt cttcttaggc atgttttatc cttt
//
LOCUS       DATRSD010000015        27091 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRSD010000015 DATRSD010000000
VERSION     DATRSD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27091
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.015"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            1..188
                     /locus_tag="VBD85_000543"
                     /pseudo
     CDS             1..188
                     /locus_tag="VBD85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(324..394)
                     /locus_tag="VBD85_000544"
     tRNA            complement(324..394)
                     /locus_tag="VBD85_000544"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Thr,seq:ggt)
     gene            complement(465..770)
                     /locus_tag="VBD85_000545"
     CDS             complement(465..770)
                     /locus_tag="VBD85_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7133117.1"
                     /translation="MFPNLLSLISYHEQQIASRRLSHHLGLEQSIVLYAMAKEETISN
                     ERCPHPKLINVLEGKLEIILGDQKHILEKDSLISLSPQEIHHLRAIENSKFLQIELD"
     gene            complement(772..1110)
                     /locus_tag="VBD85_000546"
     CDS             complement(772..1110)
                     /locus_tag="VBD85_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7133118.1"
                     /translation="MSYIEKIEANKVLDLKQIVPIEEEQMLSKTLVQRKDLGMTVFSL
                     DKGQEIGRHSSPGDAMVNILSGLAQITIDQDKFDVAAGECIVMPANIPHSLYAKEAFQ
                     MLLVVVKPEV"
     gene            complement(1131..1886)
                     /locus_tag="VBD85_000547"
     CDS             complement(1131..1886)
                     /locus_tag="VBD85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7133119.1"
                     /translation="MSHKEIGHQFLARLGKKRLRPGGVQATNWLIKKGHFSADSRVLE
                     VACNQCTSSIDIAKAFSCHITALDLDSKVIEKAKENVKKAQLEEFIEVIQGNALKLPF
                     PDNSFDIVINEAMLTMLSNSAKEKAIKEYLRVLKPGGRLLTHDVSYQDEDTAKLIDQL
                     RHTINVNVAPLKIDDWQNLFLQNGFSSSEYQFGKMSLMSPLGMIKDEGLFNTIKIISR
                     GLKKENRSQFLKMKHFFTKTGKNLCYIAVASTK"
     gene            2104..4145
                     /locus_tag="VBD85_000548"
                     /pseudo
     CDS             2104..4145
                     /locus_tag="VBD85_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            4260..4709
                     /locus_tag="VBD85_000549"
     CDS             4260..4709
                     /locus_tag="VBD85_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7133120.1"
                     /translation="MIEKLIYNDLISLNHRYRDQTNLLEVLSQYLLQKGYVKTEFSKA
                     ILQREKDYPTGLQLENMAVAIPHTYSEYVLKPFIYINKLKEPISFIQMGTEDEIVMAR
                     YVIVLGISNPKDQAGLLAELMTLFSNPKIVQQLEMAQTKEALKNIFN"
     gene            4728..5006
                     /locus_tag="VBD85_000550"
     CDS             4728..5006
                     /locus_tag="VBD85_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133121.1"
                     /translation="MKKLIVACGSGVATSQTVASKVDRLLKEAGISCDIEAVDMKSLE
                     QHLKTADAYISIVKAKKDYGVPVFNGVAFLTGMGQKQELEKIISFVKG"
     gene            5018..6355
                     /locus_tag="VBD85_000551"
     CDS             5018..6355
                     /locus_tag="VBD85_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7133122.1"
                     /translation="MDVLQTVINYILDLGAAVFVPFLMLIIGLCMKMKFRDAFTSALI
                     LGIAFTGMGILVNYIMTSMGAAANDLTKHTGLSLPAVDIGWPGAASISWAWPYAFLVF
                     PLQLAINFVMLLLNQTKTLNVDLWNVWNKIFTAVIVTYFTNNVIFGFIAAAIIIILEL
                     KLGDVFAPEVERLTGVPGVTVPHFICLIAVILHPIDELLKKIPVLNKDFDADTLKEKI
                     GVFGENSVMGAIIGFVLGIASGNGIKFALTLAVQAATALTLFPMVSKLFSQALSPISE
                     AVSDFMREKFEDREVYIGLDWPILGGRNELWVAVIFTIPFLLIGSIILPGNIVLPFAG
                     IINLSFVVGALLLTNGNVIRMIIHGLISTPLFLYGATYFTPYMTRLAKETGTLKQKGL
                     ISWATFEGPDIRYLLATVFQGKVLSVILLIIWLALYVWLLKERKKYNTNLIVE"
     gene            6378..7208
                     /gene="rhaD"
                     /locus_tag="VBD85_000552"
     CDS             6378..7208
                     /gene="rhaD"
                     /locus_tag="VBD85_000552"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEO7133123.1"
                     /translation="MTYPDYINELLEVTYDMWQHGWDEYNGGNVSYRLTKEEIGTLRH
                     DLEDTLFTYNDSQEEVDVLSVPDNMQEEYLLITASGSHFRTLKNQPEIDTGIIRITST
                     GYQVIAGFNSGKKPTSEIFMHILAHSARVSQNPNHRVVIHNHATNIVIYSLLEEITSR
                     RLTIDLWSVLTESIVVFPDGIAVLPWEVPGTYQIGLDTAHELKKHRLVVWAKHGVLST
                     GVSYQDCFGLIETADKAANIALKLRSTSGKSIQDNNILTTDDLEKVCQKLQVKGRYLL
                     "
     gene            7343..7807
                     /locus_tag="VBD85_000553"
     CDS             7343..7807
                     /locus_tag="VBD85_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7133124.1"
                     /translation="MLEDKVIVFQKSHLDSSEEALDYVSHLLLQSGAVKESYPNAIQE
                     REKAFPTGLMFSDYGVAIPHTDIEHVNYSQMAIVSLKNPVVFQQMGNGEDVEVNIIFM
                     LALKEAHQQVDLLSQLIELFQNKVLLKTLLALGNERSAIDQALGLLKAQGIE"
     gene            7820..9151
                     /locus_tag="VBD85_000554"
     CDS             7820..9151
                     /locus_tag="VBD85_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7133125.1"
                     /translation="MLDILQKFVNLGAIVVLPILIFIFGILLGTKPRKAFISGVTVGI
                     GFVGLNMVVDLLGNSLGPAAQAMVKRFGLHLTTIDVGWPAASAISYGTLLGSLAIPIG
                     VGINLLLLFLGMTKTLMVDMWNFWHAAFVASLVYAVTHNFALGLYATVVYMVMIYLLG
                     DIIAPMIKTFYGFPNITFPHGTSAPGVIFAIPLNWLFDRIPGFNKIDAKPETIQKRFG
                     VFGDTTVMGLIIGIVMGLLANYNVTNTLQLGIKTAAVMVLMPRMVALLMEGLTPISES
                     ANTFVQKRFPGREVNIGMDSALSVGHPAVLSSSLILIPITILLSVILPGNTTIPFGDL
                     ATIPFAVCLMAAVFGGNIIRTVVGGTIYMAVIMYITSWVAPLVTLSAKAANFDLSGHS
                     RITALVEGGLWPTWLFVFAAKTLPWLILTLILIISLSVMYYLNKVKKEGEI"
     gene            9153..9437
                     /locus_tag="VBD85_000555"
     CDS             9153..9437
                     /locus_tag="VBD85_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133126.1"
                     /translation="MKKLLIMCGSGIATSTVVVGKVKTWLEENHYQDQVRIYQSKIAE
                     EINHIDDYDIVISTTVVPDNVQDKVIMGLPLLTGMGVDAVWSELEAQIKD"
     gene            complement(9722..10552)
                     /locus_tag="VBD85_000556"
     CDS             complement(9722..10552)
                     /locus_tag="VBD85_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7133127.1"
                     /translation="MNHKELHLFNNLSYIDLYPIQFGKEACAPLHSFGPSLKEHYLFH
                     YIVSGRGTFYNTEEETAHKLEAGQGFLIPPHLVCSYEADKADPWTYIWIEFDGLKSDY
                     FLKQAGLDHKHLIFSQEKNSFDSPVYTEAINILASHNQRSAKVIAHLYLFIDALISQS
                     INHKVIPHNETKDFYIREAINFIERNFQFPITVEEIAKSCNLNRHYFCRLFREQTNSS
                     PQQFLIQYRLSKACALLKNRNLNLSDIAEQIGYSNQFNFSAAFKRQYGISPRQWRKTL
                     "
     gene            10791..12068
                     /locus_tag="VBD85_000557"
     CDS             10791..12068
                     /locus_tag="VBD85_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEO7133128.1"
                     /translation="MKKMGYYMGLAAACLALLAGCSNNSNKESKASDNKSGTTEISYA
                     IWDSAQEPGIRKIADKFEEKNPKIKVKIQVVSWDAYWTMLEAGATGGSLPDTFWMHSN
                     EIYRYGANKMLLSLDDYIKKSKEVKLSNYPKGLTKIYNIKGEQYAIPKDYDTIGLWYN
                     KKMFDAAGVSYPDETWDWNKLKEAAKKLTKPDGSQYGFLAPLHNQEGYYNFVYQNGGT
                     IITKDKKSGYDDPKTVEALKYYLSFVKENLSPKVTEDKKRAEVMQNGQAAMGYFGSWN
                     LNSFASNDYMKENFDVAVLPKANDGKRSTIFNGLGNAISAKTKHPQAAWKWVEYLSSK
                     EAQDMQAKLGVAISAYKGSDKVWVDSNKTFAIKHFVEMIDYAQIRPYSNATTKWEDKV
                     YEELKPAYLGKESLEKAVKKITKTMNRALEREK"
     gene            12152..13042
                     /locus_tag="VBD85_000558"
     CDS             12152..13042
                     /locus_tag="VBD85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7133129.1"
                     /translation="MKNKKKWNEYLWGWALVAPTIIGLMVLNIIPIFQTLKMSFFKSG
                     DFGQNDIFVGLDNYKRLIEDEQVWQATWNTFKYTLLVVPLTVILALVLATLLNSKIKG
                     KHVYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINFLLRKIGLNGLNWIEDPNIALYS
                     IALIGIWSTVGYSMILILAGLQEIPKDFYESATIDGASAVKQFFSITLPLISPTLFFV
                     VVTSIIQAMQVFDAIYLMESVTSPAYNKTVSLVYLFYNNSFKYADQGYGSTIVMLLLV
                     IILIITLIQMKVQKKWVYYS"
     gene            13056..13889
                     /locus_tag="VBD85_000559"
     CDS             13056..13889
                     /locus_tag="VBD85_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7133130.1"
                     /translation="MYKTKKKFPYLWAHVFLIFVALGMIIPFIWMVLTSFKTITESTQ
                     LNPFHFFPSKWQVSNYTEVIRTNNFPILYFNTIMMMLWRIVSSVLFSAMAAYAFARLK
                     FPGRNLLFGLVLFQMMVPPQLFVIPQYLMVNQLNMRNTIFALVFPGLVSAFGTFLLRQ
                     FFIGLPDELEEAAKLDGCNIGQTFFKIMLPLAKPGLIALGIFTALFAFKDLLWPLIIN
                     SESDAATLSSALSKIQGAYAVNYPQLMAASVLAIWPMVVLYIIFQKQFIQGISTSGGK
                     L"
     gene            13899..16100
                     /locus_tag="VBD85_000560"
     CDS             13899..16100
                     /locus_tag="VBD85_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEO7133131.1"
                     /translation="MPINFREKEKEFHLYNDSLSYVICVLPNGHIGNLYYGKRLSENM
                     SYAYLLENKYRPLTAYVYEDDDCFSLQHTRQEYASYGSGDMILPAFEISHEDGSHLSH
                     FVFYDYAISKGKPQLSGLPNTYVESDEEATSLELYLKDHNSETELCLTYTIYESRAVI
                     TRNAKFTQRGEKELCLKRALSLCLDLPDKDYEWLHLDGAWGRERYLQSSLLHQGSQSI
                     YSLKGASSAEHNPFIALKRPDANEHEGEVMGFSLVYSGNFLAQVDVTPFHQTRVSMGI
                     HPLGFEWYLKSGESFQTPEVVMVYSHLGLNGMSQIYHDLFSQRLARGEWRDRERPILL
                     NNWEAMTFNFNENSILSLAEVAAKLGVELFVMDDGWFGQRNNEKAGLGDWRVNQKKLP
                     NGLLGISQRIHQLGMLFGLWIEPEMVNKDSDLYREHPDWILHHPKHSQSQGRHQYTLD
                     LSREEVYQNIYQQLHDLLANHGIDYIKWDMNRYMTEAYSYHATKEHQGAIFHRYILNL
                     YRLYETLTSEFPHILFESCSSGGARFDPGMLYYAPQTWTSDDTDAYERLKIQYGTSLV
                     YPLITMGNHVSEVPNQQLLRTTPLSTRANVAYFGSFGYEIDLMLLEEEELELIKKEID
                     FYKNYRRVFQFGNFTRLINPFETDDAAWQVISEDGHHVFVGLYLGLLKLNGPSYHRLH
                     LKGLEAEASYRLNNQVYLGSALMNAGLVINEKDFTEGYKDFASAIYHLEKM"
     gene            16190..17362
                     /locus_tag="VBD85_000561"
                     /pseudo
     CDS             16190..17362
                     /locus_tag="VBD85_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275277.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
     gene            17372..18856
                     /gene="galT"
                     /locus_tag="VBD85_000562"
     CDS             17372..18856
                     /gene="galT"
                     /locus_tag="VBD85_000562"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEO7133132.1"
                     /translation="MMKTLLDKFVDQLIEIGTFEELDRIYLNNRIMALVGEEGLDQQT
                     DAESLIDIKDKLVDIAVKNGACQELLSKKDMLGAQLMDFITPLPSQVNAAFWKTYKEN
                     PKQAIADFYQLSKANDYIKVKANAKNIYYQVTTDYGNLEITINLSKPEKDPKAIAEAK
                     LSKECHYPKCQLCLENEGYQGRIDYPARTNHRVIRYEINKEIWGFQYSPYAYFNEHCI
                     FFYGKHQPMKISSKTFERLLDIVDRFPGYFAGSNADLPIVGGSILTHEHYQGGRHTFP
                     MEVAEIEEDLTFKGFKKVRAGIVKWPISVIRLRSENQQQLIALATKILENWQNYSDES
                     VHILSESSGIPHHTITPIARKKGNSFELDLALRDNQTSTEFPDGMYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKEELGLVERYLLGEVNEVVSYHQKWADNLKLVSEITSGNVE
                     QIVKDAVGKVFSRVLEDAGVFKRTPEGKAAFRRFVESVGVIKGE"
     gene            18858..19853
                     /gene="galE"
                     /locus_tag="VBD85_000563"
     CDS             18858..19853
                     /gene="galE"
                     /locus_tag="VBD85_000563"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7133133.1"
                     /translation="MAILVLGGAGYIGSHMVDYLLTKTDETVVVVDNLVTGHREAIHL
                     EAIFYKGDLADKTFMRQVFVENPSIDTVIHFAAYSLVAESMEQPLKYFNNNTAGMINL
                     LEVMTEFAIKFIVFSSTAATYGIPNDIPIKEITPQRPINPYGESKLMMETIMKWADAA
                     YGVKFVPLRYFNVAGAKPDGSIGEDHKPETHLLPIILQVAQGKREKVMIYGDDYQTSD
                     GTNIRDYVHPFDIAAAHLLAVDYLRKGNPSTAFNLGSSIGFSNFQILEVARRVTGKDI
                     PVEVTKRRIGDPDILIADSSKARDILGWQPEFDDIEKIISSAWAWHSSHPDGYKS"
     gene            19979..20980
                     /locus_tag="VBD85_000564"
     CDS             19979..20980
                     /locus_tag="VBD85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEO7133134.1"
                     /translation="MTIIKKNFGEKLAFLYELKNKNGMKVTLTNFGARIVDIFIPIDE
                     HLRNISLSATSVEDYYQKDIYVGATVAPVAGRISGAKTHIKGKCYHFTENEPNRTLHS
                     GVDTSVEQYWSTEIEDKENKIIFSLFLPDGKNGFPGNVTVIATYQLTEENELKVNYQA
                     QSDCDTIFNPTNHVYFNLTGDFQKSVASHQLKISSQEFVPLGKDHLPTGEILTVEGTD
                     FDFREFALFSKGLNRFSFQTRLVNGFDHPWLLEVGNNAVEVVSPDNKISLIMKTNQPA
                     VVIYTYNHGPTALAACHGAFSLECQSLPNACNLENLGSILLEKGKVFDSHFSYRFVF"
     gene            21171..21425
                     /locus_tag="VBD85_000565"
     CDS             21171..21425
                     /locus_tag="VBD85_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO7133135.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDRDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            21534..21845
                     /locus_tag="VBD85_000566"
     CDS             21534..21845
                     /locus_tag="VBD85_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133136.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGVVFA
                     K"
     gene            22113..22691
                     /locus_tag="VBD85_000567"
     CDS             22113..22691
                     /locus_tag="VBD85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133137.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSQRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWEGRK"
     gene            22688..23380
                     /locus_tag="VBD85_000568"
     CDS             22688..23380
                     /locus_tag="VBD85_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133138.1"
                     /translation="MNDFFNWLITLYNDDKVAFVSLCLTLVFGFFSWLAQLKRNKQDD
                     IRQAEQDEFNRLAQDELKEFQSFQKKFAEYQQKVSELQFNFESQENFKPYFEFSKTKS
                     STYVEEGKLIARICLTNVGRGTATKITTNPIDYDQDGNPMYFRTIPTKQTHAIYDYFS
                     ETFAIPNDSIFLSTTALEQTENQNHCIEFKIKFCDSSGRTYEQKFEFGYDNFLVRGIN
                     LKSVTYEPTLIK"
     gene            23402..26053
                     /locus_tag="VBD85_000569"
     CDS             23402..26053
                     /locus_tag="VBD85_000569"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO7133139.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNRGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLEALGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKI
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(26271..27050)
                     /locus_tag="VBD85_000570"
     CDS             complement(26271..27050)
                     /locus_tag="VBD85_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133140.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHNVIEEICLYLIAKESILYFESLDENSHLQLKELLDYN
                     DEWPFDIFDDMDSYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
BASE COUNT         8753 a         4308 c         5343 g         8687 t
ORIGIN      
        1 gttaacaaat tcttggcaag ctcattggaa aattagaaat agaatctaag atgtcatcaa
       61 caggtgctag acatacttat agaagttatt tgttggctaa cggagtagat atttgggcag
      121 ttgcaaaatt aatggggcat aaagatatca aacaattaat agaaacatat ggtcatttgc
      181 ttctgtaagt ggaagaaaag gaaaatgatc agcttttaaa aattcttaat aataaaggta
      241 gtttttaaaa tgactatcaa gaacaaatta agaacaaaaa taaaagaaac cctattataa
      301 taaggtttca ctcatatatt ttctgccacc accgagaatt gaactcggaa ctgagcatta
      361 ccattgctct attataccat ttaactatag cggctacttt tctatgatac tataagattt
      421 tgacgaaatc aagtatttct ttacttagaa aaatagtcgt ttttttagtc taattctatt
      481 tggagaaatt tactattttc aatagctctt aaatggtgta tttcttgtgg ggagaggcta
      541 ataaggctat ctttttcaag gatatgtttc tgatcaccaa ggataatttc aagttttcct
      601 tccaaaacat ttattaattt tggatgagga catcgttcat tgctgatagt ctcttcttta
      661 gccatagcgt acaaaacgat actttgttcc agacctaggt gatgacttaa tcgacgacta
      721 gcgatttgtt gctcgtgata agaaataagt gatagtaaat ttggaaacat cttacacctc
      781 aggcttcaca acgacaagaa gcatttgaaa ggcttcttta gcatagagac tgtgaggaat
      841 gttagcaggc attacaatac attcaccagc tgctacgtca aatttgtctt gatcaatcgt
      901 gatctgcgca agtccactaa ggatatttac catagcgtct ccgggagatg agtggcgtcc
      961 aatttcttgc cccttgtcta atgaaaagac cgtcattcct aaatctttcc gttgaactaa
     1021 agttttacta agcatttgct cttcttcaat aggtacgatt tgttttaaat caagaacttt
     1081 attggcttca attttttcga tataagacat aataactcct ttttaatagt ctattttgta
     1141 ctagcgacag caatatagca gagattttta ccagttttag taaagaaatg tttcattttt
     1201 aaaaattggg aacggttttc ctttttcagt cctcggctga tgattttaat ggtattgaat
     1261 aaaccttcat ctttaatcat tcctaatggg gacataagac tcatttttcc aaattgatat
     1321 tctgaacttg aaaaaccatt ttgtaagaat aaattttgcc aatcatctat ttttagagga
     1381 gcaacgttaa catttatagt atgtctaagt tgatcgatca gcttggcagt gtcttcatct
     1441 tgataagaaa catcatgtgt gagtaaacgt ccaccaggtt ttaggacacg taagtactct
     1501 tttatggctt tctccttagc tgaattacta agcatcgtta gcattgcttc gttgataacg
     1561 atatcaaaac tattatcagg aaagggcagt ttgagtgcat ttccctgaat aacttcaata
     1621 aattcttcta attgagcctt tttgacattt tctttggctt tttctattac tttactatct
     1681 agatcaagcg ctgtaatgtg acaggaaaat gccttagcta tatcgatgga actggtacat
     1741 tgattacaag ctacttctag aacacgactg tcagcactaa agtgtccttt tttgataagc
     1801 caattcgtcg cttggacacc tcctggtctt aagcgttttt ttcctagtcg tgctaagaat
     1861 tgatgtccaa tttctttatg tgacaataag atcacctctt tcttttaata gtaatatcat
     1921 tttttcttaa ctataacaca tagatgctaa gataacaagg agtaatagtg attgaatggt
     1981 ataaatgatg aattttagaa aacgttttct ttgtaattag gaatgtggta tcatgaaaat
     2041 gagtggcgct acgctagaca gcatttcttt aagcacgagg ttactatgaa taagagaacg
     2101 tttttgttat tacaacaact agcacaatca aggctagcaa ctttagagca attagcaact
     2161 caatttaaaa cttcaacacg tacgattcga aaagatattg atatgattaa cgattgtttg
     2221 attaaagcag gagctgattt aatttatttt ggtaatgagg gtgaagtttg ctctgacaaa
     2281 gccaaaaatc atttgaacga tattcctaaa atgatttctc tcacagacta taggttatct
     2341 agggaagaaa ggttaatttt agaatcttat tttctattag ttagcttgac tcctataacc
     2401 atcaagggct taagtcgaaa aatgtatttg agtcgtaata ctgtggttaa tgatttacct
     2461 tcattacgct ctttttgacg caaaacgaat taattttgaa atcagataaa caaggtttaa
     2521 tcattgaagg ttctgaatcg cagaagcgtt tattgttgat gcatcattat atttcactaa
     2581 gtgataacga ttttctcaga gaatatctat ttcctagtga agatgaccca tcgttattaa
     2641 tttcagtagt tcgtaaagct gagaaacaga atcaggttgt ttttcgcgat tactcatttc
     2701 aatatgttca aacttatttg aatgttttat gttctcgttc tgctaacaac ttgatgattg
     2761 aaacggaagg aatgtttcat ttttctgcat tttccttgat tattagtcaa tcaatattag
     2821 aggatttatc tcaaaaagtt agatttccct tatcgcttaa ggaggttgag tttttagctt
     2881 ttctaattgg aaatttgact tttgtgaaga aatcttctcc gattgatgat agttttaaaa
     2941 tccaattttt aactagacaa tttatcaagc aaatatcagg agatattgca tatgatttga
     3001 ctcaggacat tagtttgcta gaaaatttgt ctaagcatat ggagacgatt ttttataacc
     3061 aacctttaca agctcctgat tcgtcattgc taaccgatat cattgaatcc aataaaagca
     3121 ttgtcacagc cattaaaaag catttgtcta tcttccatat tctcaaatct aaggtatttg
     3181 aacccaatga tatctattac attgctattc atatttgtgg tgctgtggag cgtcagcgtt
     3241 tggaaatagc acattttaaa gttttactgg cttgtcatgc gggtataggg acatcaatgt
     3301 tattgaaaca acgtttaaaa gagcacttta actttgatat tatagatgtt gttaccattc
     3361 atgaagcaga ggaagagcag catcgagctg attttattat aacgacggtt ccactccaaa
     3421 agacaaataa tgaaacgatt gttgtctctg cttctttaac agaaacagat attatcagaa
     3481 tcaatacatt aatgagaaaa atcagtaagg aaggcaacgt agaagaaaat ccggaaacca
     3541 gtcaggcttt atttaaagat ttgttgccaa tactaaaaaa aataggttgt gagcaaaaag
     3601 atttggttat gattcggcaa gttataaatc gtcactgcca gaaatctatg agcccatttg
     3661 aggagtcaat ggctcatcag gttattcaat tatcagattt attaacacct aattatattg
     3721 acttgcaagg gaaagcgtcc tcgtgggagg agtctataag gcagtcggct aggctcctac
     3781 gggaagagtc attaattgag gaatcatatg tagaagctat gattgcaaat gtactttctt
     3841 atggtccata tatattggtt gcaaagggag tagctgttgc tcatgcaggc attgatgacg
     3901 gtgttaatgg tttgggaatg tcgctttatc gcttagataa gggagtaaat tttggcaatc
     3961 cagaattgga tcccatccgt tatatcattt gtttatgtgt aacagattac ggtaagcatg
     4021 tgactgcttt taatactctg cttaatttat ttcaggatag tagtatgaga gaaaagttgg
     4081 atagggttga tactgctaaa gattgttatt gtctgattaa aaaatacgaa gaaaaggaaa
     4141 attagtaaga ccaaaatact tcataaaaac tgaagtattt tggtcttttt gtgtgaacat
     4201 tttaaattta taataaagac agtaaaagct atcttgcaga aggaggagaa ctattgtcta
     4261 tgattgaaaa gttaatctac aatgatttaa tttctttaaa tcaccgttat cgagatcaaa
     4321 caaatttact agaagttttg tctcaatact tattgcaaaa aggctatgtt aaaacagagt
     4381 tttcaaaagc tattttacag cgagaaaaag attatccaac aggactacag ttagaaaata
     4441 tggctgttgc tattcctcac acctattcag agtatgtcct taagcccttt atttatatca
     4501 ataaattaaa agaacctatt agtttcatac aaatgggaac agaggacgaa atagtgatgg
     4561 cacgttacgt catcgtttta gggatttcaa atcctaaaga tcaagcaggt ttactagctg
     4621 aattaatgac actcttttct aatcctaaaa tagttcaaca actcgaaatg gcacaaacca
     4681 aagaagcctt aaaaaatatt tttaactaat tgatggaggt atttattatg aaaaaattaa
     4741 ttgtagcttg tggttcggga gttgcgacct cacaaactgt tgcatctaaa gtagaccgtt
     4801 tgttaaaaga agcaggaatt agttgcgaca ttgaggcagt agatatgaaa tctttagagc
     4861 aacatttaaa aacagctgat gcttatattt cgattgttaa agccaaaaaa gattatggtg
     4921 ttcctgtctt taacggcgtt gctttcttaa caggaatggg tcaaaagcaa gaattggaaa
     4981 aaattatttc attcgttaaa ggttgaggag tagcgttatg gatgttctac aaactgttat
     5041 taattatatt ttagatttag gtgctgctgt ttttgttcct tttttaatgc ttattatcgg
     5101 tttatgcatg aaaatgaaat tccgtgatgc tttcacatct gcacttattc tcggaatagc
     5161 ttttacaggg atgggaatcc tggttaatta cattatgact agtatgggtg ccgcagcgaa
     5221 tgatttaacg aaacataccg gtttaagttt accagcagtt gatattggat ggcctggagc
     5281 agcctctatc agttgggctt ggccttatgc ttttcttgtt ttcccattgc agctggctat
     5341 taattttgtc atgttactct tgaatcagac caaaacactt aatgtggatt tatggaacgt
     5401 ttggaataag atttttactg ctgtaattgt aacttatttt accaataatg ttatctttgg
     5461 ttttattgca gcagctatta tcattatttt agaattaaaa ttaggagatg tctttgctcc
     5521 agaagttgag cgcttaacag gtgttccagg tgtcacagtc ccacatttta tttgtttaat
     5581 tgctgtaatt ttgcatccta ttgatgaact attgaaaaaa attcctgttt taaataaaga
     5641 ttttgatgca gatacactaa aggaaaaaat aggagtgttt ggagaaaatt cagttatggg
     5701 agcgattata gggtttgtct tagggattgc ttctggtaat gggataaaat ttgcgttgac
     5761 cttagctgtt caagctgcaa cagccttgac tttatttcct atggtttcaa aattattttc
     5821 tcaagcgctt tccccaattt ctgaggcagt gtcagatttt atgagagaaa aatttgaaga
     5881 tcgggaagtt tatattggtt tggattggcc aatactaggt gggcgaaatg aactttgggt
     5941 ggctgttatt tttaccattc cattcttatt aataggttca attattctac cagggaatat
     6001 tgttttacca tttgcaggga ttatcaatct ttcatttgtt gtaggagctc tattgctcac
     6061 aaatggcaat gtaatccgaa tgattatcca tggacttatt tcgactcctt tattcttata
     6121 tggagcaact tatttcacac catatatgac tagacttgct aaggaaactg gtactctaaa
     6181 gcaaaagggt ctaatttctt gggcgacttt tgagggccca gacattcgat accttttggc
     6241 tactgttttt caaggtaaag tattatctgt tattttactt atcatttggt tggcattgta
     6301 tgtttggcta ttgaaagaac gcaaaaaata taacacgaat ttgattgtgg aataaaagag
     6361 aagacagagg gaaaaatatg acatatccag actatattaa tgaacttttg gaagtcacgt
     6421 atgatatgtg gcaacatggt tgggacgaat ataatggcgg taatgtgagt taccgattaa
     6481 ccaaagaaga aattggtact ttaagacatg atttagaaga taccttattt acttataatg
     6541 acagtcaaga agaagtagat gttctatctg taccagataa tatgcaagag gagtaccttc
     6601 ttatcactgc ttctggtagt cattttagaa cattgaaaaa tcaaccagag attgatacag
     6661 gcatcataag gataacttct actggctatc aagttatagc aggctttaat agtggaaaga
     6721 aaccaacaag cgaaattttt atgcatatac tagcgcattc tgcgagggta agtcaaaacc
     6781 ctaaccaccg tgttgttatt cataatcacg ctactaatat tgtcatttat tccctattag
     6841 aagaaataac tagtcgacgt ttaacgatag atctttggtc agttctaaca gaatcaatag
     6901 ttgtttttcc tgatggcata gcagttttac catgggaagt tccaggaact tatcaaatag
     6961 ggttagatac agcacacgaa ttaaagaaac atcggttagt cgtttgggca aagcacggtg
     7021 tcctttcaac aggagttagt tatcaagatt gttttgggtt gattgaaaca gcagacaagg
     7081 cagctaatat tgcacttaaa cttagaagta ccagcggtaa gtcaattcaa gataataata
     7141 tattgaccac agatgatttg gaaaaagtat gccaaaagtt gcaagtaaaa gggcgttact
     7201 tattataatt agctcatgaa tgatggataa tagtaacagg agaccaaaat gcttcacttt
     7261 gagtgaagta ttttggtctt tttatgaaat cgattccata ttataataaa accaagaaaa
     7321 cctaagtaaa ggagaggcgc tgatgctcga agataaagtc attgtatttc aaaaatcaca
     7381 tttagattct tcagaagaag cattggatta tgtatcccat ttattgctac aatctggtgc
     7441 tgttaaagaa agttacccca acgctattca ggagcgtgaa aaagcatttc ccaccggtct
     7501 aatgttctca gactatggtg ttgcgattcc tcatactgat attgagcatg ttaattattc
     7561 acagatggca atagttagtc ttaaaaatcc tgtagttttt cagcaaatgg gtaatggaga
     7621 agatgttgaa gttaatatta tctttatgtt agctttaaag gaggctcatc aacaggttga
     7681 tttattgtcg cagttaattg aactttttca aaataaagta ttacttaaga cattattagc
     7741 tctaggtaat gaaagaagtg ctattgacca ggctcttggt cttttaaaag ctcaaggaat
     7801 tgagtaggag gtctttttaa tgttggatat tttgcagaaa ttcgttaatt taggagcaat
     7861 agtcgttttg ccaatactga tatttatctt tggtatttta ctgggaacga aacctagaaa
     7921 agcatttatt tctggggtga ctgttggtat tggttttgta ggattaaata tggttgttga
     7981 tttactaggt aatagtttag gtcccgcagc acaagctatg gttaaacgat ttggcctaca
     8041 tttaacaacg attgatgtcg gatggccagc agcctcagca atttcttatg gaaccctttt
     8101 aggaagctta gccattccga ttggtgttgg tattaacctt ttactcctat ttttaggaat
     8161 gactaaaaca ttaatggtag atatgtggaa tttttggcat gcagcttttg tcgcctcgct
     8221 tgtttacgca gtaactcata attttgctct aggtttatat gccacagttg tttatatggt
     8281 tatgatttat cttctaggtg atattattgc gcctatgatc aaaacatttt atggcttccc
     8341 taacattact tttccgcatg gaacctcggc tccaggggta atatttgcta ttcctttgaa
     8401 ctggttgttt gaccgcatcc caggtttcaa taaaatagat gccaaacctg agactattca
     8461 gaagcggttt ggagtttttg gtgatacaac agtaatgggg ttaatcattg gtattgttat
     8521 gggacttctg gctaattata atgtcactaa tactcttcag ctaggtatta agacagcagc
     8581 agtcatggtt ttaatgccac gaatggttgc ccttttaatg gaaggattaa cgcctatttc
     8641 agagtcagca aatacttttg ttcaaaaacg ttttcctggt cgtgaggtca atattggaat
     8701 ggattcagcg ttatcagttg gacatcctgc tgttttatct agttctttaa ttttgattcc
     8761 gataactatt ttattatcag tcattttacc tggtaatacg accattcctt ttggcgattt
     8821 agcaaccatc ccatttgctg tttgtttgat ggcagcagta tttggtggaa atattatacg
     8881 tacggtcgta ggaggtacta tctacatggc agtgattatg tatatcactt catgggttgc
     8941 tcctttagtg acattatctg caaaagcagc taattttgat cttagtggtc attcacgaat
     9001 cacagctcta gtagaaggtg gtttatggcc aacgtggtta tttgtatttg cagccaagac
     9061 actaccgtgg ctgatactaa ctcttatttt gatcattagt ttatctgtta tgtattatct
     9121 caataaggtt aaaaaagaag gagaaattta agatgaaaaa attattgatt atgtgtggct
     9181 cagggattgc aacgtcaaca gttgtggttg gaaaagtaaa aacatggctc gaagaaaatc
     9241 actatcagga tcaagtgcgt atttaccaat caaaaatcgc tgaagaaatc aaccatattg
     9301 atgattatga tatcgttatc agtacgacag ttgttccaga taatgtccaa gataaagtta
     9361 ttatgggttt accgctatta actggtatgg gggtcgatgc tgtttggtca gaacttgaag
     9421 cacaaattaa ggattaataa aagaagagta atgttttttg taagtaaggc tgatacatga
     9481 ttttagcctt acttttttct tttgagataa caattgcatc aattagttaa aagaggccat
     9541 gtctgaggga aataagaaga ctcttttact ggcaacttgg ttgtgcgatg caaaagagtc
     9601 gattgttatt aattactttg attattctat tttttgatag gttccttgtt tattaaccat
     9661 tataatatgg tgatattata gggttagtgt agtaaatctt agctgatatt taacgtttag
     9721 tctaaagagt tttacgccac tgtctaggag atattccata ctgacgtttg aaagcagcag
     9781 agaaattaaa ttggttagag tagccgatct gttcggctat atcactgagg ttcagattac
     9841 ggttttttag caaagcgcat gccttactca agcgatactg aattaaaaat tgttgaggcg
     9901 agctattggt ttgttctcgg aagagacgac aaaagtaatg cctatttaaa ttacaggatt
     9961 tggctatttc ctcaacagta ataggaaatt gaaaattacg ttcaatgaag ttaatggctt
    10021 cacggatata gaaatccttt gtttcattat gaggaataac tttatggtta atagactgac
    10081 taattagggc atctataaaa agatagagat gcgcaatgac ttttgcactt cgctggttat
    10141 gacttgctaa gatattaata gcttctgtgt agactggact atcaaaagaa ttcttttctt
    10201 gcgagaagat caagtgttta tggtctaaac cagcttgctt tagaaaataa tcacttttta
    10261 gaccatcaaa ttcaatccag atataggtcc aggggtcagc cttgtcagcc tcgtaactac
    10321 aaactaagtg aggaggaatg agaaaacctt gccccgcctc caatttgtga gcggtttctt
    10381 cttcggtatt ataaaaggta cctcgacctg atacgatata gtggaaaaga taatgctctt
    10441 ttaatgaggg tccaaaagaa tggagaggag cacatgcctc tttcccaaat tgaataggat
    10501 ataaatcaat ataagagaga ttattgaata aatggagctc tttgtgattc attattaatt
    10561 tcctcaaagt ttaatacaaa ttaaagcgtt tacttaagcg tattataaca tagtttatat
    10621 gagaatgtga ttatttatat gattaagtgc taatatgacc ataaattcat ataaataaag
    10681 taaaacatta gcattcaaat gtatgcgctt acatgataaa ataaacttgt ttcaatgagt
    10741 tagaaaataa gcttttctaa ccaactatat taaataatag gagtgtagct atgaaaaaaa
    10801 tgggttacta tatgggatta gcagcggctt gccttgcact attagctgga tgttctaata
    10861 acagtaataa agaatccaaa gcgtctgata ataaatcagg aacaacagag atatcatatg
    10921 ctatttggga ttctgctcaa gagcctggaa ttagaaagat agctgataaa tttgaagaaa
    10981 aaaatcctaa aattaaggtg aaaattcaag tggtttcttg ggatgcttat tggacaatgc
    11041 ttgaggcagg agcaacaggt ggatcattgc cagatacttt ctggatgcac tctaatgaaa
    11101 tttatcgtta tggtgcaaat aagatgcttt tgtcacttga tgattacatt aaaaagagta
    11161 aagaagtcaa gctcagcaat tatccaaaag ggttaactaa aatctataat atcaaaggtg
    11221 agcaatacgc aataccaaaa gattacgata ccattggttt gtggtataac aaaaaaatgt
    11281 ttgatgcagc gggggtttca tatccagatg agacatggga ttggaacaaa ttaaaagaag
    11341 ctgctaaaaa actaactaag cctgatggta gtcaatacgg ctttttagca ccgcttcaca
    11401 atcaagaagg ctactataat tttgtttatc aaaatggtgg taccattatt acaaaggaca
    11461 aaaaatcagg atacgatgac cctaaaacag tcgaagcctt gaaatactat ttaagttttg
    11521 ttaaagagaa tttatcacca aaggtgacag aggataaaaa acgtgctgaa gtgatgcaaa
    11581 atggacaagc agccatgggc tacttcggct catggaacct caattctttt gcttcaaatg
    11641 attatatgaa agaaaacttc gatgttgcag ttctaccaaa agcaaatgat ggtaaacgtt
    11701 caacgatttt caatggtttg ggtaatgcta tttctgccaa aacaaaacat ccacaagccg
    11761 cttggaaatg ggttgaatac ttaagttcaa aagaagccca agatatgcaa gccaaacttg
    11821 gtgtggcaat ctctgcttat aagggttcag acaaggtttg ggtggattct aataagactt
    11881 ttgctataaa acatttcgtt gaaatgattg attatgcaca aattcgacca tattcaaatg
    11941 ctacaactaa gtgggaagat aaagtctatg aagaattaaa acctgcatac ttaggcaaag
    12001 aatcactaga aaaagccgtt aagaaaatta ctaaaacaat gaatcgagcg cttgagcggg
    12061 aaaaataaga tactgaaaaa actctggaga tgggatttaa gggaaaatat ttccaatctc
    12121 gtctccagtt tttaactaat gaggtacaaa gatgaaaaat aagaaaaaat ggaatgagta
    12181 tttgtggggg tgggcattgg ttgctcctac gattattggt ttaatggtct taaacattat
    12241 tcctattttt caaacgttaa agatgagttt ttttaagagt ggtgattttg gacagaatga
    12301 tatttttgta ggacttgata attataagag gttaattgag gatgaacaag tttggcaagc
    12361 cacatggaat acttttaaat atactctctt agtagttccc ttaactgtta ttttggcgct
    12421 agttttagca acgcttctca actcaaagat taaaggtaaa catgtttatc gtacaatctt
    12481 tttcctacct atggttgctg ctccagcagc ggtcactatg gtttggaaat ggctttataa
    12541 tacagatttt ggcttaataa actttctttt acgtaaaata ggattaaatg gtttgaattg
    12601 gattgaggac cctaatattg ccctatactc catcgcttta attggtattt ggagcactgt
    12661 aggttatagt atgattttga tcttagctgg cttacaagaa attccaaaag atttttacga
    12721 atccgctacg attgatggtg ccagcgctgt taaacaattt ttctctataa cgctaccttt
    12781 aatctcccct actctattct ttgtggtagt tactagtatt attcaagcaa tgcaagtttt
    12841 tgatgctatt tacctaatgg agtctgttac aagtccggcc tataataaga ctgtttcgtt
    12901 ggtctacctc ttttacaaca attcttttaa atatgccgac caaggttacg gatccactat
    12961 cgtaatgtta ttacttgtta ttattttgat tattacctta atccaaatga aggtacagaa
    13021 aaaatgggta tattatagtt agaaagggta tcaatatgta caaaacaaaa aagaagtttc
    13081 cttatttgtg ggcacatgtg tttttgattt ttgtagctct aggaatgatc attcctttta
    13141 tttggatggt cctaacttcc tttaagacca ttacagaatc aacacaacta aatccatttc
    13201 atttttttcc aagtaaatgg caagtgtcca attacactga agtaatcaga acaaacaatt
    13261 ttcccattct ttatttcaat actattatga tgatgttatg gcgtatcgtt agttcagtat
    13321 tatttagtgc tatggcagca tatgcatttg ctcgtcttaa atttcccgga agaaaccttt
    13381 tatttggatt agtcctattt caaatgatgg ttcctccaca attatttgtt atcccacaat
    13441 atctaatggt taatcaacta aatatgcgaa atacgatttt cgcgttggtg tttccggggt
    13501 tggttagtgc ctttggtacc ttcttacttc gtcagttctt tatcgggcta ccagatgagt
    13561 tagaagaggc tgctaaacta gatggttgta atatcgggca gactttcttt aaaattatgc
    13621 ttcctctagc aaaaccaggt ttaatcgctt taggaatttt cacagctcta tttgccttta
    13681 aagacctttt gtggccttta attatcaatt ctgaatcaga tgcagctact ctttcaagtg
    13741 cattatcaaa aatacaagga gcttatgctg ttaattatcc acaactaatg gcagcaagtg
    13801 tacttgctat atggccaatg gtagttcttt atattatatt tcagaaacag tttatccaag
    13861 gaatttcaac ttccggtggg aagttatagg agagaagtat gccaattaat tttcgtgaaa
    13921 aagaaaaaga atttcatctc tataatgaca gcctcagtta tgttatttgt gtcttgccaa
    13981 atgggcatat tggaaatctt tattatggga aacgattatc agaaaatatg tcctatgctt
    14041 atttattaga aaataaatat cgaccactta cagcctatgt ctatgaagat gatgattgtt
    14101 tttcgttaca acatacgcgt caagaatatg cttcttatgg cagtggagat atgattttac
    14161 cagcttttga aattagtcat gaagatggta gtcatttatc acattttgtt ttttatgatt
    14221 atgctatttc aaaggggaag cctcaattat ctggtttacc taatacctat gtagaatctg
    14281 atgaggaagc aactagtctg gagctttatt taaaggatca taatagtgaa acagaacttt
    14341 gtttaactta taccatctat gagagtagag ctgttatcac aagaaatgct aaatttaccc
    14401 aaagaggcga aaaagagctt tgtttaaaaa gagcccttag cctttgccta gacttacctg
    14461 ataaggatta tgaatggctt catttagatg gtgcttgggg aagggaacgt tacctacaaa
    14521 gttcgctatt acatcaaggg agccaatcaa tttacagttt aaagggtgct agtagtgccg
    14581 aacataatcc ttttatagct ttaaaaagac ccgatgcaaa tgagcatgag ggagaagtaa
    14641 tgggcttttc cttagtatat agtggaaatt ttttagcaca ggttgatgtg acaccatttc
    14701 atcaaactag agttagtatg gggattcatc cgttaggatt tgaatggtat ttaaagtcag
    14761 gagaaagttt ccaaacccct gaggttgtta tggtttacag tcatttagga ttgaatggca
    14821 tgagtcaaat ctatcacgac ttatttagcc aacgtttggc tagaggagaa tggcgtgata
    14881 gagaacggcc tattcttctt aataattggg aagctatgac atttaatttt aatgaaaatt
    14941 ccattttaag cttagctgaa gtagctgcta agcttggtgt agaacttttt gttatggatg
    15001 atggctggtt tggtcaaagg aataatgaga aggctggcct tggagattgg cgtgtgaatc
    15061 aaaaaaaact tcccaatggt ttacttggta ttagtcagag aatccatcag ttagggatgt
    15121 tatttggttt gtggattgaa ccagagatgg tcaacaaaga tagcgatctc taccgagaac
    15181 atcctgattg gattttacat catcctaagc attcacaatc tcaaggacgc caccagtata
    15241 cactagattt gtctcgggaa gaggtatatc aaaatattta tcaacagtta catgatctgt
    15301 tagctaatca tggtattgac tatataaaat gggacatgaa tcgttatatg acggaggctt
    15361 acagctatca tgcgactaaa gagcatcaag gagcaatctt tcatcggtat atcctaaacc
    15421 tttaccgact atatgagacg ttaaccagtg agtttccaca tattctattt gagtcatgct
    15481 ctagtggtgg agcaaggttt gatccaggaa tgctttatta tgctccgcaa acatggacta
    15541 gcgatgatac ggatgcttat gaacgtctaa aaattcagta tggtacaagt ctggtttatc
    15601 ctcttataac tatgggaaat catgtttcgg aggttccaaa tcaacaactc ttacgtacca
    15661 caccgctttc tacaagagct aatgtagctt attttggaag ttttggatat gagattgact
    15721 taatgcttct tgaagaagag gaattagagc taatcaaaaa agagattgat ttttacaaaa
    15781 attatcggag agtcttccaa tttggtaatt ttacgcgtct aattaatccg tttgaaactg
    15841 atgatgctgc ttggcaagtc atctcagaag atggacatca tgtttttgta ggtctctatt
    15901 taggtttgct aaaactaaat ggtccttctt atcatcgttt acacttaaaa ggtttagaag
    15961 cagaagcaag ttatcgttta aataatcaag tctatctagg aagtgccttg atgaatgctg
    16021 gcttagttat caacgaaaaa gacttcacag agggctataa agattttgca tcagctatat
    16081 accatttaga aaagatgtaa aaagctcgaa ctaactttca caacacactt gttagtgctt
    16141 tgttagtagc taacaatagt aaatgtaata gaaggagtga aaaataatta tgactgaaaa
    16201 agaaatacag acagcttttg caaatgtttt tcataatgag gcagagagtt ttttcttctc
    16261 accaggacgt atcaatttaa ttggagaaca tactgattat aatggagggc atgtctttcc
    16321 agctgctata acattaggaa catacggagc tgccaggaaa agaaatgatt aattattacg
    16381 cttttactca agtaactttg aagacattgg tgtgattgaa gtcaatttga ataatcttgt
    16441 ttacaataaa gctgataatt gggctaatta tgctaaaggt gttcttaagt ttttacaaga
    16501 agcaggacat actatcgata ccgggatgga tatttacgtt tctggcaata tccctaacgg
    16561 ttcaggttta tcttcatctg cttcgcttga attattgatt ggcattatgg cagaagagat
    16621 ttttaatctt aaattggatc gtcttgattt agtaaaaatc ggcaagcaga cagaaaataa
    16681 atttattggt gttaattctg gtatcatgga tcagtttgct attggaatgg gagctgataa
    16741 acaggccatc tatcttgata ccaacagttt agcatacgat ttagttccgc ttgaactggg
    16801 agacaatatt gtagttatca tgaataccaa taaacgtaga gaattagcag attctaagta
    16861 taatgagcgc cgagcagaat gtgaaaaagc agtggaagaa ttacagattg ctcttgacat
    16921 taaaactttg ggagacttag atgaagcgac atttgatgag tattcttatt tgataaaaga
    16981 tgctaatcgt ctcaaacgag cccggcatgc ggtatctgaa aatcaacgca ctttaaaggc
    17041 aaaagaagct cttatatcag gagatttatt acgatttgga cgactaatca atgcttctca
    17101 tgtttcgtta gaacatgact atgaagtgac tggaatagag ttggacacac tagttcatac
    17161 tgcgtgggag caagatggcg tgcttggagc tcgaatgact ggagcaggtt ttggcggttg
    17221 tgctattgct atcgtagcta aagataatgt aaaacaattt acagcaaatg tcggtaaaat
    17281 ctatagtgaa attattggtt attcacctag tttttatatt gcagaaattg ccggaggtag
    17341 ccatattctt acttgtaaat aaggaaataa tatgatgaaa acattattgg ataaatttgt
    17401 agatcagctt attgaaatag ggacctttga agaattggat aggatttacc tgaacaatcg
    17461 tattatggct ttagtaggtg aagaaggctt ggatcagcaa acagatgcag agagtttgat
    17521 tgacatcaaa gataaacttg tagatatagc cgtgaaaaat ggtgcttgtc aggaactatt
    17581 atctaagaaa gacatgctcg gtgcacagtt gatggatttt ataactccat taccaagtca
    17641 agttaatgct gctttttgga agacctataa agaaaaccct aagcaggcta ttgctgattt
    17701 ttatcagttg agtaaagcaa atgattatat caaagttaaa gctaatgcta aaaacatcta
    17761 ttatcaagtg actacagatt atgggaattt agaaattacc atcaacctct cgaagcctga
    17821 aaaagaccct aaagctattg cagaagctaa gctttctaaa gaatgtcatt acccaaagtg
    17881 tcaactttgt ttagagaatg aaggttatca aggtcgtatt gattatcctg cacgtactaa
    17941 ccatcgtgtt attcgatatg agataaataa agaaatctgg ggattccaat attcacctta
    18001 cgcttacttt aatgagcact gtattttctt ttatggaaaa caccagccta tgaagattag
    18061 ttctaagaca tttgagcgtc tcttagatat tgtcgatagg tttcctggat attttgcagg
    18121 atcaaatgct gaccttccta ttgtcggtgg ctctatttta acccatgagc attatcaagg
    18181 aggtcgccac acatttccaa tggaagtagc agagatagag gaagacctta ctttcaaagg
    18241 ttttaaaaaa gtaagagcag gtattgtaaa gtggccaata tcagttatac gcttgcgaag
    18301 tgaaaatcag caacaattaa tcgctttagc aacaaagatt ctagagaatt ggcagaatta
    18361 ttcagatgag tctgttcata ttttatctga gagttcaggt attccacacc atacaattac
    18421 acctattgct cgaaaaaaag gtaatagttt cgagttagat ttggccttac gtgataatca
    18481 aacatcaaca gaatttccag atggtatgta tcatccacac cctgatgttc aacatattaa
    18541 gaaagaaaat attggcttaa tagaagtcat gggattagcg attttgccgc cgcgtctcaa
    18601 ggaagaactt ggtttagtcg aaaggtatct tttaggggaa gttaacgagg ttgtctccta
    18661 tcaccaaaag tgggcagata atttaaaatt agtaagtgag attacctcag gaaatgttga
    18721 acagattgtc aaagatgctg taggtaaagt gttttcccgt gtacttgagg atgcaggagt
    18781 attcaaacga actccagaag gtaaagcggc ttttaggaga tttgtggaga gcgtaggagt
    18841 cataaaggga gaataaaatg gcaattttag tcttaggtgg tgcaggttat atcgggtctc
    18901 atatggtaga ttacttactg acaaagacgg atgagacagt tgttgttgta gataatttag
    18961 tgaccggaca tagagaagca atacacttag aagcgatttt ctacaaggga gatttagcgg
    19021 ataagacttt tatgagacag gtatttgtag aaaatcctag tattgacact gtaattcact
    19081 ttgcagccta ttctctggta gcagaatcta tggagcagcc tttaaaatat tttaataata
    19141 atacagcagg catgattaat ctcttagaag ttatgactga atttgctata aaatttatcg
    19201 tattttcttc aactgcagca acttatggta ttccaaacga cataccaatt aaagaaataa
    19261 cgcctcaacg tcccattaac ccatatggtg aaagtaagtt gatgatggaa actattatga
    19321 agtgggctga tgctgcttat ggggttaaat ttgttccttt gcgctatttt aatgttgcag
    19381 gagcaaaacc ggatggttca attggtgaag atcataaacc tgagactcac cttttaccaa
    19441 tcattcttca agtagcacaa ggaaaacgcg agaaggttat gatttacggt gatgattatc
    19501 aaacatcaga tggaaccaat attcgtgact acgttcatcc atttgatata gccgctgctc
    19561 acttactagc ggttgattat cttcgtaaag gcaatccttc aactgcgttt aatttaggtt
    19621 cctctatagg attctctaat ttccaaattt tagaagtagc tcgcagagtg actggtaaag
    19681 acattcctgt tgaagtgaca aaacgtcgta ttggggatcc agatatcctt atagccgatt
    19741 ctagcaaagc ccgtgacatt ttgggttggc agccagagtt tgatgatatt gaaaagataa
    19801 tctcttcagc atgggcttgg cattcgagtc acccagatgg ctataaatcc taaagaataa
    19861 agctttaacc atatgataag aaatttactt caagcagatt agtatttttg gattagaaag
    19921 agtcttatta gcataatgaa tttatcaggt atcattagtt tgaaagaaga aaaaatatat
    19981 gactattatt aagaaaaact tcggagagaa gctggcattc ctctacgaat taaaaaataa
    20041 aaatggaatg aaggtcactt tgacgaattt tggtgcacgt atagttgata tcttcattcc
    20101 gattgatgaa cacttgcgta atattagttt atcggctact tctgttgaag attattatca
    20161 aaaagatatt tatgtggggg caacggtggc ccctgtggca ggtagaattt ctggagctaa
    20221 aacccatatc aaaggaaaat gttatcattt tactgaaaat gaacctaata ggacattaca
    20281 tagtggtgtt gatacatctg tagaacagta ttggtctaca gaaatagaag ataaagagaa
    20341 taaaattatc ttttctctct ttcttccgga tggtaaaaat ggctttcctg ggaatgtgac
    20401 tgtgatagct acttatcagt tgacagaaga aaatgaactc aaggtcaact atcaagcaca
    20461 atctgattgt gataccattt ttaacccaac aaatcatgta tattttaatt taacagggga
    20521 ttttcaaaaa tccgttgcta gccatcagtt aaagatttca agccaagagt ttgtgccact
    20581 agggaaagac catttaccta ctggtgaaat actgactgtg gaaggtacag actttgattt
    20641 tagagaattt gccctttttt caaaaggtct aaatcgcttt tctttccaaa cgagattagt
    20701 aaacggattt gatcatccgt ggcttttaga agtaggcaat aatgccgttg aggtagttag
    20761 tcctgataat aaaataagct taataatgaa aaccaatcaa ccagctgttg ttatttacac
    20821 ctataatcat ggtccaacag cactagcagc ttgtcatgga gcttttagtt tagaatgcca
    20881 atctcttcct aatgcttgta atctagaaaa cttaggatca attttgctcg aaaaagggaa
    20941 ggtatttgat agccactttt cttatcgttt tgtgttttaa tatgacatct aaaagatgtg
    21001 atgccaaaac tagaatggca tagagaacca tacatttcaa taggtcttcc ttagataaaa
    21061 ctagttgttt ttatatctgt cagaaaaatc ataatttatt tttgatataa tgattttttg
    21121 agtatttaat ttatatgtta taatgaaaaa aactaaaaaa gaggaatatc atgtcatacg
    21181 aacaagaatt tttaaaagac tttgaagagt ggttacaatc gcaaataagt atcaaccaaa
    21241 tggcaatgga cagtgcaaag aaagttctag aggaagatag ggatgagcga gcagcagatg
    21301 cgtatattcg ttatgagagt aagttagacg cttatcgctt tttgcaaggg aaatttaata
    21361 actatcataa ccaaaaatct ttccatgatt tgcctgatgg tctttttgga caacgtcatt
    21421 attaaaaggt ttatcgctag aaagcttgtc aaataggcat ttctatgata cattgttata
    21481 gtagttttta gatactatga tagatttcat aaatgaggag aaagcattat acaatggcaa
    21541 agcgtattaa accgaataaa aaaagagagc ttttgaaagc taaagggatc aattggaagg
    21601 atgttcgact atctcaaact ttacaagaag tcgcatcacc gaaaaaaaag gctcctgaag
    21661 cgccaaaggc agtcgatctt ttagaaggtt taactgctaa taaacaagat gttttaaaag
    21721 aagctggcct tgtcagcctt gaagcgtttg cgaaagtaag tgaagcagat gttttagcac
    21781 taaaaggtat tggtccagca gctattaaac agttagttga taatggtgtt gtttttgcaa
    21841 aataatttga tttaccttgc gaaagtagcc ttattttgct agaataaatt aacttagagg
    21901 tgttttgaaa tcccatttgt cacaggaagc ggtcatactg agaaatccgc acaaatgtca
    21961 aagcttgttg ctaagtccaa taattgaaac aaaacgagtt gcggatgtta tcatccgaat
    22021 ttgggtggta ccgcggaaat cgtaatacga aattcgtccc tgtcagaatt gacagggatt
    22081 ttttgtattt aggagtgagg agattgaagt atatgcagat tctagaagat ttcgatggca
    22141 aagcattacc aaaactagaa acggatcggc ttatcttgcg tcagcgaacg gtgggggatg
    22201 tcccagccat gtttgattac gtttgcttgg aagaggttgc ctatccagca ggactcagcc
    22261 caatagcttc tttagaagat gagtacgatt attttgaaaa tcgctactat caaaatctgg
    22321 aaaaggcaaa attaccatct ggttatggta ttaccgttaa aggaagtgac cggattatcg
    22381 gctcttgcga ctttaaccac cgccatgaag acgatgtttt cgagattggc tacatgcttc
    22441 atcctgacta ctggggtcat ggctatatga cagaagctgt cgcagccttg attgaagtcg
    22501 gttttaccct tctcaatctc cacaaaatcg aaatccgctg ctatgactac aataagcaaa
    22561 gccagcgcgt ggcagaaaaa ctaggattca cccttgaagc aaccatccgt gatcgtaagg
    22621 ataatcaagg taatcgctgt gtcaatttga tatatggttt gctgaggagt gagtgggagg
    22681 ggagaaaatg aatgattttt ttaattggtt aattacacta tataatgatg ataaagttgc
    22741 ttttgtatca ctttgtctaa ctttagtgtt tggatttttt tcatggttag cccaattaaa
    22801 aagaaacaaa caagatgata ttcgacaagc tgaacaggat gaatttaaca gactagctca
    22861 agatgagtta aaagaatttc agagttttca aaaaaagttt gcagagtatc aacaaaaagt
    22921 gagtgaattg cagtttaatt ttgaaagtca agaaaatttc aaaccctatt ttgaatttag
    22981 caaaactaaa tcaagtactt acgttgaaga aggaaaattg attgcaagaa tatgtctgac
    23041 aaatgtaggg agaggcacag ctacaaagat tacgaccaat cctattgact acgaccaaga
    23101 tggaaatcca atgtatttta gaacaatacc tactaaacag acacatgcta tatatgatta
    23161 ttttagtgag acttttgcta ttccaaatga ttctatattt ttgagtacaa ctgcactaga
    23221 acagacagag aatcagaatc attgtataga gtttaaaatt aaattttgtg attcatcagg
    23281 cagaacttat gaacaaaaat ttgaattcgg ttatgataat tttttagtaa ggggaataaa
    23341 tctgaaatca gtaacttatg agccaacctt gattaaataa gagaattaga aaggacacta
    23401 tatgtcaaaa gaactatcac caaaatacaa tcctgccgag gttgaggcag gccgttatca
    23461 aacttggctt gaccaagatg ttttcaagcc atcaggggat gctgaggcta agccttattc
    23521 tattgtgatt ccaccaccaa acgttacggg taaattgcac ttgggtcacg cttgggatac
    23581 aactctacaa gatatcatta ttcgtcaaaa gcgtatgcag ggctttgata cactctggct
    23641 tccaggtatg gaccacgctg gtatagccac tcaagctaaa gttgaggagc gccttcgtga
    23701 gcaaggcatt tcacgttatg atcttggtcg tgaaaaattc ctagataaag tttgggaatg
    23761 gaaagacgag tatgcagcaa ctattaaatc tcagtggggg aaaatgggtc tttcggttga
    23821 ttatagccgt gagcgtttta cccttgatga gggactttca aaagcggtaa gaaaagtctt
    23881 tgttgatttg tacaatagag gttggatcta tcgtggggag ttcatcatta attgggatcc
    23941 agcagctcgt acagcccttt cagatattga agttattcac aaggatgttg agggtgcttt
    24001 ctatcatatg aattatatgc tggaagatgg ctcacgcgct ctagaagtag caacaactcg
    24061 tcctgagact atgtttggag atgtggctgt agcggtaaat ccagaagatc cacgttacaa
    24121 ggatttgatt ggtcaaaatg ttatccttcc tatcatcaat aaacctattc caatcgtcgc
    24181 tgatgaacac gctgatccag aatttggaac aggtgtggtt aagattacgc cggctcacga
    24241 ccctaatgac ttcgctgtag gtcaacgcca caacttacca caagttaacg ttatgaacga
    24301 tgatggtact atgaatgaac ttgcggatga atttaatggt atggatcgtt tcgaagctcg
    24361 taaggcagta gtcgctaaat tagaagcgtt aggaaactta gtaaaaattg aaaaaatgac
    24421 tcattctgtt ggtcattcag aacgaacagg cgttgtggtt gaaccacgtt tatctactca
    24481 atggttcgtc aagatggatc aattggctaa gaatgccatt gccaaccaag atacagaaga
    24541 caaggtagag ttttatccac cacgatttaa cgatactttt atgagctgga tggaaaatgt
    24601 ccatgactgg gttatctctc gtcaattatg gtggggacac caaattcctg catggtacaa
    24661 cgtcaatggt gagatgtatg ttggagaaga tgccccagag ggtgacggat ggacacagga
    24721 tgaagacgtt ctagacacat ggtttagttc tgctttatgg ccgttttcaa caatgggatg
    24781 gccagacacc gaagcagctg acttcaaacg ctacttccca acgtcaacct tggtaactgg
    24841 atacgatatc atcttcttct gggtatcgcg catgattttc caatcccttg aatttacagg
    24901 acgccaacca ttctcaaatg ttcttattca tggactcatc cgtgatgaag aaggacgtaa
    24961 aatgtctaaa tcacttggca atgggattga tccgatggat gtcattgaga aatatggtgc
    25021 ggatgccctt cgttggttcc tctcaaatgg ttcagcccct ggtcaagatg tgcgtttttc
    25081 atacgaaaaa atggatgctt cgtggaattt cattaataag atttggaaca tttcacgcta
    25141 tatccttatg aataacgaag gtttaacgtt ggatcaagcc agagaaaatg tggaaaaagt
    25201 tgttaatagt caagttggaa atgtaacaga ccgttggatt ctccacaatt taaatgagac
    25261 tgtgggtaaa attactgaaa actttgacaa gtttgaattt ggtgttgctg gtcacatcct
    25321 ttacaacttt atctgggaag agtttgctaa ttggtacgtt gagttaacaa aagaggttct
    25381 ttatagtgac aatgaagatg agaaagttgt tacacgttct gttcttcttt ataccttaga
    25441 tcaaatcttg cgtcttcttc atccaatcat gccatttgtg acagaagaaa tttttggaca
    25501 atatgcagaa ggatcaattg tccttgcaag ttatccacaa gttaacgcga catttgaaaa
    25561 tcaaacagct cacaaaggcg ttgaaagctt gaaagatttg attcgttccg tgcgtaatag
    25621 cagagcagag gtcaatgttg ctccatctaa accaatcact atcttggtta agacaagtga
    25681 cagcgaactc gaaagcttct tcaaagacaa tagcaactac atcaaacgct tcacaaaccc
    25741 agagacgctt gaaatcagct cagcaattgc cacaccagaa cttgccatgt catcagtcat
    25801 cacaggagct gaaatcttcc tcccacttgc tgacctcctc aacgtagaag aagagttggc
    25861 acgacttgaa aaagaacttg ctaaatggca aaaagaacta gatatggttg gcaaaaaact
    25921 cagcaacgag cgctttgtag ccaacgctaa accagaagtt gtccaaaaag aaaaagacaa
    25981 acaagccgac taccaagcta aatacgacgc aacccaagaa cggattgtag agatgcaaaa
    26041 acttgttaaa tagttaaaat catccattac ttccgcagag tcctgccgct agaccatcag
    26101 tctaagcgac agggcttttt gctatattca aagattgtga aaaattaata tatatgatgc
    26161 ctacttcttg cgtctgaaat acaagaagtg aaaatgattt ggccataaag aaaaacaggc
    26221 agaaacccaa atgattaatg cctatttctt atattttttc gtcaattatt ttataaataa
    26281 aattgaggta caaaccagtt tttaaaatga taaggtgaat cttcttcaat atagatgtct
    26341 gtatataaaa aggtatagct atccatatca tcaaaaatat caaaaggcca ctcatcgtta
    26401 taatctaata attccttaag ttgtagatgg gagttttcgt ccagagattc aaaataaaga
    26461 atggattctt ttgcaattaa atagagacaa atttcctcta ttacattatg cgctgtataa
    26521 gatgaaccaa ctttatttct tgatgtatat tgagccaaaa ctttgcttaa tttataaaca
    26581 aactcataat catattttgt gataaattgt tgaggcaata gtgataaaag tagggaagta
    26641 tctaattgtc ccagatgact ttctttaggt agtttagata attcctccaa atcattatta
    26701 atatcatcag aaagtatttc acaaccagca ataaatgaaa gagcaggagt aagtcccata
    26761 gtctggtaaa tcttttcagg ccaaagttta ttttcctttg cttcctcaaa taatttatct
    26821 ataaaatcac attctttctc tttagggaat ctctccaaaa ataataagtc aagtatgata
    26881 tccttagatt tcaaaggaat tgccccacca tttttttcaa atgattccca atctgattta
    26941 gacatattgg atatttctgc caaatcagtt gacgacacgc caacttctaa cctacgatgg
    27001 tgaatgtagt tagcaagtga cttactaata ataaaatcag aatttagcat tccgttctcc
    27061 ttttgattcc tattcattat gattatcaca t
//
LOCUS       DATRSD010000016        26971 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRSD010000016 DATRSD010000000
VERSION     DATRSD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26971
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.016"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            82..2583
                     /locus_tag="VBD85_000571"
     CDS             82..2583
                     /locus_tag="VBD85_000571"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7133141.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITKENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QDLALANEKVQSEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2673..3281)
                     /locus_tag="VBD85_000572"
     CDS             complement(2673..3281)
                     /locus_tag="VBD85_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7133142.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVTAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTNLSTNEKNRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3374..4762)
                     /locus_tag="VBD85_000573"
     CDS             complement(3374..4762)
                     /locus_tag="VBD85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7133143.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFVTIKKKTYYVMTKDYSGILVDGSIKKVPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDEHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(4759..5445)
                     /locus_tag="VBD85_000574"
     CDS             complement(4759..5445)
                     /locus_tag="VBD85_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133144.1"
                     /translation="MTKIIYFADDEKNIRDLVVPFLEHDGFTVRAFETGDLLLEAYKN
                     QKPDLVILDIMMPGTNGLDVMKSIRQYDNVPIIMLTARDSDVDFITAFNLGTDDYFTK
                     PFSPIKLSLHVKALFKRLDEKAIKNDTQYQFLDLTLDTEKRIALLSNEEMPLTKTEFD
                     FLLVLIEKPETAFSRETLLNRIWGFDDIESRAVDDTIKRLRKKFKQYHSQVSIKTVWG
                     YGFKLIKEDK"
     gene            complement(5678..10393)
                     /locus_tag="VBD85_000575"
     CDS             complement(5678..10393)
                     /locus_tag="VBD85_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7133145.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVAESHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLIGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASTIPAIFTQKEFGDVLAQKSYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKGQPHLAISP
                     NDDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESDIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     KSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            10716..11408
                     /locus_tag="VBD85_000576"
     CDS             10716..11408
                     /locus_tag="VBD85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO7133146.1"
                     /translation="MEKGFQDGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITITVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLVTDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLATFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            11398..11721
                     /locus_tag="VBD85_000577"
     CDS             11398..11721
                     /locus_tag="VBD85_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO7133147.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKCLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            12091..14328
                     /gene="metE"
                     /locus_tag="VBD85_000578"
     CDS             12091..14328
                     /gene="metE"
                     /locus_tag="VBD85_000578"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO7133148.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNIIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENLAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     KLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRGNLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDVKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            14373..16217
                     /locus_tag="VBD85_000579"
     CDS             14373..16217
                     /locus_tag="VBD85_000579"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO7133149.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNLAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLATGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHFGPYHMIQSLKQV
                     PLFAQSYLSVYPNASQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLK
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNYFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            16564..17271
                     /locus_tag="VBD85_000580"
     CDS             16564..17271
                     /locus_tag="VBD85_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO7133150.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(17436..18521)
                     /locus_tag="VBD85_000581"
     CDS             complement(17436..18521)
                     /locus_tag="VBD85_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7133151.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(18722..19261)
                     /locus_tag="VBD85_000582"
     CDS             complement(18722..19261)
                     /locus_tag="VBD85_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO7133152.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLERRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVIENYPQKIKILKHQRDL
                     EQYLKELPVASNTDPLKSL"
     gene            20030..20797
                     /gene="cfb"
                     /locus_tag="VBD85_000583"
     CDS             20030..20797
                     /gene="cfb"
                     /locus_tag="VBD85_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO7133153.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLANKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            20970..21272
                     /locus_tag="VBD85_000584"
     CDS             20970..21272
                     /locus_tag="VBD85_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7133154.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(21429..22292)
                     /locus_tag="VBD85_000585"
     CDS             complement(21429..22292)
                     /locus_tag="VBD85_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7133155.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARHRISLHSNVREADSINSKQYTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(22597..23157)
                     /locus_tag="VBD85_000586"
     CDS             complement(22597..23157)
                     /locus_tag="VBD85_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO7133156.1"
                     /translation="MRITIFCGASTGENPVYSEKTVSLAQWMAQNKHSLVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVGEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(23154..23471)
                     /locus_tag="VBD85_000587"
     CDS             complement(23154..23471)
                     /locus_tag="VBD85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7133157.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            23685..24299
                     /locus_tag="VBD85_000588"
     CDS             23685..24299
                     /locus_tag="VBD85_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7133158.1"
                     /translation="MNKKSFFQCVILFVSFLVLTLAVESQSDMISYLDNITSAFFQSI
                     RNPDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
     gene            24548..25624
                     /locus_tag="VBD85_000589"
     CDS             24548..25624
                     /locus_tag="VBD85_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7133159.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQMIQKLNDLKKDY"
     gene            25626..25832
                     /locus_tag="VBD85_000590"
     CDS             25626..25832
                     /locus_tag="VBD85_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO7133160.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGSIAFLILEGNNE"
     gene            25829..26731
                     /locus_tag="VBD85_000591"
     CDS             25829..26731
                     /locus_tag="VBD85_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133161.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            26728..>26971
                     /locus_tag="VBD85_000592"
     CDS             26728..>26971
                     /locus_tag="VBD85_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133162.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPEPTYIDSYIQYFKNMNQLGLVI"
BASE COUNT         8460 a         4427 c         5021 g         9063 t
ORIGIN      
        1 ttgagggtat tttcaccttt tttattgcta tttttcgtgt tataataaga agactatact
       61 tttttgacat ggagaagtgc gatgacattc tataatcaca aagaaatcga acccaaatgg
      121 caagcctttt gggcagataa tcatactttt aaaacaggaa cagatgcatc aaaacctaag
      181 ttttatgccc tagatatgtt cccataccca tctggagctg gtctccacgt tggtcaccca
      241 gaaggctata cagcaactga tattctcagc cgtttcaaac gtgcccaagg tcacaatgtc
      301 ctccacccaa tgggttggga tgcctttgga cttcctgctg agcagtacgc tatggatact
      361 ggtaatgacc cagccgagtt tacagctgaa aacatcgcta actttaagcg ccagattaac
      421 gctcttggct tctcatacga ctgggatcgc gaagtcaaca caactgaccc taactactac
      481 aagtggacac agtggatctt tactaagctt tatgaaaaag gcctagccta tgaagctgaa
      541 gttcccgtta actgggttga agaattggga acggccatcg ccaacgaaga ggttcttcca
      601 gatggcacat cagaacgtgg cggctaccca gttgtcagaa aaccaatgcg ccaatggatg
      661 ctcaaaatca ctgcctatgc agagcgcttg ctagaagact tggaagaagt tgactggcca
      721 gaatcaatta aggacatgca acgcaactgg attggtaaat caactggtgc caatgtaacc
      781 ttcaaggtta aggatacaga taaggacttc actgtcttca caacacgtcc tgacaccctt
      841 tttggtgcaa cttacgcagt tcttgctcct gaacatgcct tggttgatgc tattacaaca
      901 gctgaccaag cagaagctgt tgccgaatac aaacgtcaag caagtcttaa atcagatctt
      961 gcccgtacag accttgctaa agaaaaaaca ggtgtctgga caggtgccta tgctattaac
     1021 ccagttaatg gtaaagaaat tccagtttgg attgcggact atgtccttgc cagctacggt
     1081 acaggtgcta tcatggctgt tccagcccac gatgaacgtg actgggaatt cgcaaaacaa
     1141 tttaagctag acatcattcc agttcttgaa ggtggtaatg ttgaagaggc tgcctttaca
     1201 gaagacggtc ttcacatcaa ctcagacttc ctagatggtc tcgataaagc gtcagctatt
     1261 gccaaaatgg tcgaatggct tgaagcagaa ggtgttggta atgagaaagt cacttaccgt
     1321 ctccgcgact ggctcttcag ccgtcaacgt tactggggtg agccaatccc aatcatccac
     1381 tgggaagatg ggacatcaac agcggttcct gagagtgaat tgccactggt tcttccagta
     1441 accaaggata tccgtccttc aggtactggt gagagcccac ttgctaactt gacagactgg
     1501 ttggaagtga ctcgtgaaga tggtgttaaa ggtcgtcgtg agactaacac catgccacaa
     1561 tgggcaggtt caagctggta ctatctccgt tacattgatc cacacaacac cgaaaaactg
     1621 gctgatgagg aacttctcaa acaatggttg ccagttgata tctatgtcgg tggtgcagag
     1681 catgccgtgc ttcaccttct ctacgctcgc ttctggcaca aggttcttta cgatctcggt
     1741 gtcgtaccta ccaaagagcc attccaaaaa ctctttaacc aagggatgat tttgggaaca
     1801 agctaccgcg acagccgtgg tgcacttgtg gcaactgaca aggttgaaaa acgtgatggt
     1861 agcttcttcc atgtggaaac tggggaagag cttgaacaag caccagctaa gatgtctaaa
     1921 tcacttaaga acgttgtcaa cccagatgat gttgttgaac agtacggtgc tgacaccctt
     1981 cgtgtttacg agatgttcat gggaccactt gacgcttcaa tcgcttggtc tgaggaaggt
     2041 cttgaaggct cacgtaagtt cttggaccgt gtttaccgtt tgatcacgac taaagagatt
     2101 actaaggaaa atagcggagc ccttgacaag gtttacaacg aaaccgttaa ggctgttact
     2161 gagcaagttg accaaatgaa attcaacaca gctatcgctc agctcatggt ctttgtcaat
     2221 gcagctaaca aggaagacaa actctttagc gactatgcca aaggctttgt tcaattgatt
     2281 gcaccattcg caccacactt aggtgaggag ctctggcaag tcttgacagc ctcaggtgag
     2341 tcaatctctt acgtgccatg gccaagctat gacgaaagta aattggttga aaacgaaatt
     2401 gaaatcgttg tccaaatcaa aggtaaggtc aaagctaaac ttgtagttgc taaagacctt
     2461 tcacgcgaag aattacaaga tcttgccctt gctaacgaaa aagtccaatc cgaaatcgct
     2521 ggcaaagaca tcatcaaagt catcgcagta ccaaacaaac tggttaatat tgtggtgaaa
     2581 taagataaag attttactag aaatagtgat atttacgcgt ataaaatccc aaaagtttat
     2641 gaacttttgg gatttttcta tttttagcta aatcagtcag ctcgtactgt ttttttagca
     2701 tagtaagttt ttaatacctc ttttgtaata ccatgttttt gatagatttt ttcgattttt
     2761 gacgttaata tttttagttc tttattttgg ttttcaatag agttatcaaa tttttgatag
     2821 cggtcgttca accttgtctc aaggtaagca cgttgttcaa aatataattt taatctattt
     2881 ttttcgttag tggataggtt tgtcttatca atcaattttt tgacctcact tgtgctatcc
     2941 catgtttgat tatcaataga agtatagaat ttagttagta gacgtttatt ttgcttctct
     3001 agttggttga tatctttgtt aatatacaat tcaataactt ttaaatcttt tgcttttttg
     3061 acttcaatat gtcgttggat atggtcgatt ttagcgtaga gaggagcagc ttctttatct
     3121 gcaactttta acttatgttc aatagttttt tgactcatat gaacagttga tgttttttgt
     3181 gattgttcag ccgttacatg atttggttgt aaactcgtat atgttgctaa agagagaacg
     3241 gcaataagcg ataaggaatg tattttttta gaatgtaaca tataaaaact cactttcttt
     3301 tagaataact ttttgatcta taggtattat aggttaaaaa agcaataact tctttgaatt
     3361 tttgtaggaa aagttaggca attttgtggc aattgaaata aatcgtaaaa gttgttgagg
     3421 ttgaatcact ctcagcgatg atattaccat gatgttgctt gataatttct ttagtcattg
     3481 caagtccaat acctgtttgg ccgccatgtc ctttatagaa gcgatcgaaa atataaggta
     3541 agtcttcttt tgaaatggct ggtccgtcgt ttgagatagt cagtttttga tttgtgtaag
     3601 agatttcaat atgagaaaca gcatagcgta gtgcgttact aaggatatta ttgatagctc
     3661 gttgaatcaa ctcttcgtta ccttttagta acttttcatg ctcgtctggt ctataagtga
     3721 tactaatttt ttgtttatta gctaggggtt tgaaataagc aataatacta ttaagtaatt
     3781 catttaaaga aaattcatct ttttgatcac ttaagttact gtctaattta gagagtaaga
     3841 taatatcgtc tacgagctgt ttcatttttt tgctctcttg cagaataaca gagtgtgcga
     3901 gctcggcatc aataataccc tcttgaactc cctcagtata tccttggatg gacattaaag
     3961 gtgtccgtaa ctcgtgggag gcattttgaa agaagtttct ttggctctct tctgcctggt
     4021 agattcgatt tgccatttct tggctctcaa gtagaagagg atcgaaatca gaaaagacaa
     4081 tttctttttg actagattga tgattgcctt gatttttcca aaggctggaa atataagttt
     4141 gtactgattg aatagattct ctaattccag tgaaagtctt cctcataata aatagcatgg
     4201 gaatataggt tagtactaag ataactataa gaaaatgatt aattttagta attagatgct
     4261 gggtgtaagt aatgtctgaa aagttaatca catgaaaaag ttgcgactgt gctttaggaa
     4321 ctttttttat gctaccatca acaaggatac cagagtagtc tttggtcatt acataatagg
     4381 tttttttctt gatggttaca aacataccat ctttttcctt ggaaatttgc cacaatcttt
     4441 tttgggtaag atagcgaata atatcatggc tctctttttt atgattatca gtgccatatt
     4501 tcatacgata atgctggtca aaaatagcat agtaagacca aacaaagatg ctatcttggt
     4561 taaccatatt ttcatctggt attttcttgt tgttatagat actatctaaa atggtgaaat
     4621 tcgcctgcat ggaattcctt gtcatttgat ggacatagtc tttaatgaca tagttgacag
     4681 aaaaatagat gaggtaaaaa ccaagaaata gagtgatgct attaataagg ataagattgc
     4741 taattaaact tttctttttc atttgtcctc tttgattaat ttgaagccgt aaccccagac
     4801 agttttgata ctaacttgac tgtggtattg cttaaatttt ttacgtaatc gtttaatagt
     4861 gtcatcaact gctctgcttt caatatcatc aaatccccaa atacgattaa gcagtgtttc
     4921 cctagaaaaa gcagtttcag gtttttcaat taatactaaa agaaaatcaa attctgtctt
     4981 ggttaagggc atttcttcat ttgataatag ggctattcgc ttttcagtat ccagagttaa
     5041 atctaggaac tgatactgag tgtcattttt gattgctttt tcatcaaggc gtttgaacaa
     5101 tgctttaaca tgtaggctaa gttttatagg tgaaaaaggc ttggtgaagt aatcgtcagt
     5161 tcctaaatta aaagccgtaa tgaaatcaac atcagaatct ctagctgtta acatgataat
     5221 aggaacatta tcatattggc ggatgctttt cataacatct aaaccattag ttccaggcat
     5281 cattatgtct aatataacta aatcgggttt ttggttttta taggcttcta agagaaggtc
     5341 tccagtttca aatgctcgaa cagtgaaacc atcatgttct aaaaaaggaa ccaccaagtc
     5401 acgaatattc ttttcatcat cagcgaaata aattattttt gtcatgtata cctcaatacc
     5461 catagttata atttgacttt tagctagctg tttatgtcat atcaaatata acaaaaaaag
     5521 agtttttttc ctattctttg aagtgttata gtgttataaa acaagatact gcagagaaaa
     5581 ggtctttagg aacatcattt taagctcctc aaaaaggcac ccgtcactaa ctattaatag
     5641 ctagtaattg atgccttgct ctatttttta aagtatttta ttctcttttg atagacttta
     5701 gtttgattgt tgccagacct agcatagaga gactaaatag caatattgta taggttattt
     5761 taggagtgct ttcgcctgtt ttaggaagct tgtttgtctt taccttttga ttatcggttt
     5821 gactagtcac ccctaagaca cgcttctgag caacctcact ttctttagtc atgccttttt
     5881 tgaatatcaa cgcctggggc tgatttggcg gattgatagg tgtggtattg agagagtcat
     5941 tatcattgag tgattgacta taattctctg cggaagaaac tctatcgcga ccatcaagct
     6001 cttgttcggt tgcttttatt tggtttaata actcttcaac ttgagcttgt gtagcttgtc
     6061 tattggaaag cagcaattct gctttcttga taaggttatt ataattttct ttagcaaatt
     6121 gactggcgtt ctgatattta gatgatgttt gtttcagaac atttgcttta attatcatat
     6181 ggtgtaaacc agagtaatca gtttcatgac catttaattg atttttagca atatcaagat
     6241 tttctaaggc tttgtctact tctgattgag tgacgttagg tttttgtaga aggtttttcg
     6301 ccaattggac ttgattgata taagttgatt ggcttaatgg actagcatta acaaattgat
     6361 agtttccctt gatttctgtt tcagctaaaa tagcttttgc cagtaaagaa gtatctgttt
     6421 ctttaccgtt aagagcttgg cgactttctc tcaaagcagc aatagcatta tcaatacttg
     6481 tttgttccac tctattttta attaaagttt gtgccttttc aagattagct aggtaattat
     6541 tttttaagtg agttccagca ttataaaaca caggttgggt aataatgtca gtttgttctg
     6601 ctagggcatt aatcagagga gctttattaa tcagcgtcaa ttttgttagg tttacttgac
     6661 cttcttttac ggatacaagt aaatcatcta agttctctaa agtgctatac ccactaggca
     6721 gagttacgtt taaacgatag tctcctacag gtatgttttt accatagtca gtaggagaat
     6781 aagttgcttt tggtagatca acaacagtat ttgtcttagt aaccaactgg actgttgctc
     6841 cttttggtaa aagcttgtca aattcaacga gtaaggctgc tttcttgagt aagttaactt
     6901 taaataagac gtctttaagg ctatcttttt cagaaatagt tacagtgaca cttttgggac
     6961 tgattagatt tgctcgttcc tcatcgtata agaatagatc aaacgtatag gtaccaaaag
     7021 gcaatttcag taaattgagg tctttcccgt gatgttgctt tttgatgaca ttacctttgt
     7081 catctttgac agagtaagag aaatcaatat cgacattact gttaagctca gggctaaaca
     7141 ctttgacgtt tactaaacca tttttattgc caatattgat caaatcttcg agttttgctg
     7201 atactagatt gcctgcataa tcttcaacag tgtagtagac atgagagaaa tcgttaacct
     7261 ctttaggtag aataaagcta ccatcctttt cttgtttgat aaatactttg ttatcttcaa
     7321 caaggatacc gtcttctcct ttttgacgta agacgctatt ataatgacca tctttatctt
     7381 tttttaagta aaaaacttgt tctctaaaga tatcagattc cccgtgttct actgcaggac
     7441 gagctttgaa gatacgtcta tctttgtcat aagttgctgt tgttagtgta ggagcttgtc
     7501 tatccaaagt gatatcaaac accatttgtt gtggcttaga gccagggaca tcagagtaat
     7561 aagttaaaac gtatttgtat tttccatctt ctaaaggaat accatcagta gtggttcctt
     7621 tccattctgt gtcatataaa aatgtagatt ttggattttc agtatttccg ctgtagtaat
     7681 ttttatctcc tgcttgggga gcacttaccc atagtggttt ttgtaaatta acatcatctg
     7741 cacgatagac tttggctctt aaattattga aattacgtaa gaaaacacct ttcactgcaa
     7801 cggcatcttg attttggtca tcattaggtg aaatggctag atgaggctgg cctttttgat
     7861 ctagttgtaa gatccatttt ccatctatag acttatagct tcccaaggct ttcaattgga
     7921 taggactagt accgtctgtt gagtagatag gttctgaact tgtagtcatt aaggcagtat
     7981 aatcttctga accaggaact tcgtttgttt gttttggttg gaaataaaat cccttttctt
     8041 tgttagcaac aagcttataa atggattttt ctaaaacttc taagttttgg aattctccct
     8101 taaatccaac ataaggaata ctaagaactt ccccaccatc aacagggtct gtaaaacgta
     8161 cgtagccttc taggaaatag ccgtttggca ttactttttt taacatgtca tggtatttcg
     8221 aaacatctat atcaattgta atcgttttag tttgccctgg ttcaatccgt atcgttttcc
     8281 ctgtaaatgt accaagttgt tgaggagcca atgtgacaaa gccatcttta acttgatcag
     8341 tattcaaata ggttgtatag tgtaaatctt ttgcaacttt attgatatta tgaacagtaa
     8401 catcaaagga aattttatct ttaatatttc ctaatgtaac gctaccatag ttgttttccc
     8461 caccagttag gtataatcct gtctgaactg ctgctgcgac atcgataata cctgctcctt
     8521 gttggcgtgg tgaggtgtaa gcgcctgtat ctttgtttag gtgtgcttta gcagtactca
     8581 taagaaggta tttgacagtt ctttcaatgt cacctttttt aagttctgga tgttctttca
     8641 ataagtattg tttaactaga gcagtagcac cagcgacatg gggagaagcc atacttgtcc
     8701 cactcatcat atcatattca ttatcattga tagcggcgta aatagagcct ccaggagcag
     8761 ataagtcagg ttttaattgt ccgtctgctg ttaatcccca gcttgaaaag tcagatagga
     8821 cacctgcatt agggttagct tgtttatttt tgatattgtt aaatacaatt ttatagcttt
     8881 tttgagctaa aacatctcca aactcttttt gggtaaaaat ggcaggaata gtacttgctt
     8941 caggatctaa actcattgtt aggttagctt ccccagcttt gttattaaag ataatagctc
     9001 ccacagcacc gtgattaata gcattgacga ctttttttgt aaaagtaata tctccacgtt
     9061 caattaaggc gattttacca tttaaagttt tgtccttata atcattgtcg tttccttttc
     9121 ctacgaaaac ataatcatat tgcttcccta cttcaaaggt cttatcacta actttaggat
     9181 ctgcataagc agctaatccg ttgttgagat ttttgttgtt ttctaatccg ataatgttaa
     9241 agactttatt aaccagtgtt gtattattat atgatgctac agaaatggcc tcacgagctg
     9301 ttgatggact accaattaaa ccataatcag gataaagagc agaaggcttt gatgctccac
     9361 tcccaaatgt accgtcgtta cctgctgcta taacaacaga aacgccagcg agtctagcca
     9421 tctctaaagc ttttataagt cggtcatcgg cattaactaa agagccatta gctccaccta
     9481 aacttaaatt aatgctatca gcaccgagtt taacggcatc ttcaatagct ttaacataaa
     9541 gggctggtcc agttcctctt ttttcatcag agaagactct cataaacatt acttgtgctt
     9601 caggagcaac accatagatt aattcatttg tatctttctt gctaggatta gctgttgcga
     9661 tactggttac gtgcatacca tgagaagtgc ttttcacctc ttttagctct gtattgacat
     9721 caacatagtt gtgaccaaag attactttgt tgttatacca ttttccatag tttataccag
     9781 cttttgcttt agcagcattc atttgctgtt cgttttggta ttttgctttt gtcgaatcat
     9841 ttagttggag agcatcgtga ttggtatcta gtcctgagtc aataattgct acaacagttc
     9901 cctctccttt gtagcctgtg ttccatactt tggggacagt gataatttta ttactatcaa
     9961 tgttttgagg ggtttcttta gaaatagctg gtgtaggttt gactgctaaa ttttgtttgg
    10021 ttaaaggagt tttagaaact tcttgagtgc tttcaaccgg ttgatttgcg gctggggaaa
    10081 atgtctcggt ggcttgagag tgagactcag ctactggttg tgtttctgtt ggactattat
    10141 tattagcctt tgtaacaaga ttgtcttgag ctgtcgacgc aagggctgat gacgtgttgt
    10201 tagtaggtgt ctcatttgct atgctcgttg gactgtcttt tacttgctgt tcaactgcaa
    10261 tatcagatgg cttatttatg acagaatcag cagctacatt tgtgataccg cctaaaaaaa
    10321 atagggttcc caaaagtaca gatacggcac ctaacttata tttccggatt gaaaaacgct
    10381 gttttgtgtt cataaaaatc tccttttctt gtaacataat aattacaaaa actattaaaa
    10441 cataaaaaat caaaattttc tacaattttc tgaattttca ttacaaatta ttttgtggtc
    10501 aatttaagac cgtacctaaa gtatttttta aaaatcattc tatgaaaata ataatattgt
    10561 ccaactatat ggctacaata tcaaaggttt atagaataat tataggatat ttattaggct
    10621 aattgctggc aaagctaaat aatcgttccc cttctttctt agtaatcacc gttgtgctat
    10681 aataactgaa tatactgaaa atttaggggg atatgatgga aaaaggattt caagatggag
    10741 tgaaagatgc cttgcccaca gctttagggt atatctcaat aggcttggcg tttgggatag
    10801 ttgcttcagc atcagattta tctgcaatag aagtgggtct tatgagtgcc ttggtatatg
    10861 ggggatctgc tcagtttgct atgtgtgcgc tattattagc aaaagcagac ttgatgacta
    10921 ttacgataac agtattttta gttaatttga gaaatatgtt aatgagttta cacgcgacta
    10981 ctatcttcaa gtctgctcac ctaatgaatc aattagccat aggaactcta gtaacagatg
    11041 agagctacgg cgttttactt ggagaagcat tacatcacaa agtcgtatcg cctagttgga
    11101 tgcatggtaa taatgtgatg agttatttga catgggtaat ctcaacgata ataggaactt
    11161 tactggggag tactattcct aatccagaaa tgtttggttt agactttgca ttagtcgcca
    11221 tgtttatagg tctctttgta tttcagttgt ttgggatgtt atctgatgga aagcgacttg
    11281 ttgtatatgt tcttgcttct gtaggtttaa gttacttctt attagccact tttttgtcag
    11341 gagccttatc agtattgctt gcgactgttg ttggatgtag tgtgggagtt gttttagatg
    11401 ataaatagta ccgtttttct tgctattctt ttaggttttg ttgtttcttg ggtgccaaga
    11461 gtcgctccat tcatatttgt aaaatacaaa gggctaccaa atagtcttgt gaaattttta
    11521 aaatttcttc ctctttctat tttatttgcc ctaacattct ctagcttttt ttcagttaaa
    11581 ttaggacaat tgccaacact aaaatgctta gatttattgg ctggcatacc aactctagtt
    11641 gtcactattc gatatcgaaa tttattttac actgttctta ctggtatcgt tactatggct
    11701 ttattgagat tactatttta acgtgtagga atgggagatt aataaaaaca caaaaaacgc
    11761 atctatttct agatacgttt tttgtgtttt tgtctagaca ggaacttttg atagccgata
    11821 tgtaatagtt tgactaattg ttaataatat caacagattt ttctgtatct tgataatatg
    11881 agttgattgt aagattgctt tttaaagata tctacgtgtt gctgtttaaa aatttataaa
    11941 aaggtctata gtggtttgta tagtttaaaa ctatataaat cactaaaaat ataaaatatt
    12001 aaaagctatt gatattatag ttttcatgga ctaaaataat tcataaaact aaaagggtta
    12061 gttattagct ataacttgat ggaggaaatt atggtaaaag tttcaaattt agggtatcca
    12121 cgtcttggtg aacagcgcga atggaagcaa gcgatcgaag ctttctgggc agggaatctt
    12181 gaacaaaaag atttagaaaa acaactaaaa caattacgta tcaatcattt aaagaaacaa
    12241 aaagaggcag gtattgacct tattccagtg ggggattttt cttgttatga tcatgttttg
    12301 gatttgtcat ttcaattcaa tataatccca aagcgtttcg atgagtatga gaggaattta
    12361 gacctttatt ttgctattgc aagaggtgac aaagataatg tcgcatcatc tatgaaaaag
    12421 tggtttaata ccaactacca ctacatagtc ccagaatggg aggttgagac taaacctcac
    12481 ttgcagaata attacttact tgatctttat ctagaagcta gggaagtagt tggtgataaa
    12541 gcaaagccgg ttatcactgg tccaataacc tatgtttcct tatcatcagg aattgtcgac
    12601 tttgaagcga ctgttcagcg gttattacca ctttataagc aggtctttca agatttgata
    12661 gatgcaggcg ccacctatat tcagattgat gagccgatat ttgtaactga tgaaggtgaa
    12721 cttttagtag atatagctaa gtctgtctat gatttttttg caagagaagt accacaagcc
    12781 cacttcatct ttcaaaccta ctttgaatca gcagtctgtt tagataaact ctctaagctg
    12841 ccagtaacgg gatttggcct tgattttata catggtaggg cggaaaattt agctgctgtt
    12901 aagcaaggtc tattccgcga aaaagaatta tttgcaggaa ttgttaatgg tcgaaatatc
    12961 tgggcagtaa atttagaaga aacgttggct ttattggaag agataggtcc ctttgttaaa
    13021 cgtttgaccc ttcaaccttc ttcaagtctt ttacatgtac cggtgacgac taaatacgaa
    13081 acacatttag accctgtgtt aaagaatggc ttatcatttg ctgatgaaaa actaaaggaa
    13141 ttagaactat tagctagtgc ttttgatggt aataaaacaa agggatatca cgaagcttta
    13201 tctcgttttt cagctcttca agctgctgat tttcgtcatg tagcattgga atcattagca
    13261 gaagtaaagc ttgaacgaag tccgtataaa ttacgccaag ctttgcaagc tgaaaaatta
    13321 cagttaccga ttttaccaac aacaactatt gggtcctttc ctcaatcacc tgaaattagg
    13381 aagaaacgcc ttgcttggaa aagaggaaat ctatctgact cagattataa agatttcata
    13441 aaaactgaaa ttagaagatg gattgctatt caagaagatc ttgatcttga tgtgttagta
    13501 catggcgaat ttgagcgtgt ggatatggtt gaattttttg gtcaaaagtt agctggtttt
    13561 acgacaacca aattaggctg ggtacagtct tatggttcaa gggcggtcaa accacctatc
    13621 atttatggtg atgtcaaaca tattcaaccc ttaagccttg aagaaacggt ttatgcccaa
    13681 agcttgacta agaaacctgt taaaggcatg ttgacaggtc ctattactat aacgaactgg
    13741 tcatttgagc gagatgatat tagccgatct gatcttttta atcaaattgc tttggctata
    13801 aaagatgaga ttcaactttt ggaacaatca ggcattgcta ttatacaagt ggatgaagca
    13861 gcccttcgag aaggtttacc cttacgccag caaaagcaac aggcttactt agatgatgct
    13921 gttgcggcct ttaaaattgc aacttcatct gtgaaagatg agacacaaat tcatacacat
    13981 atgtgttatt caaaatttga cgaaattatt gattctatcc gtgcactaga tgcagatgtt
    14041 atttctattg aaacgagtag aagtcatggg gacatcattg aaagttttga aacagcagtt
    14101 tatcctctag gaattggcct gggtgtttac gatattcatt cccctcgcat acctactaag
    14161 gaagaaatta ttgtcaatat tcaacgatca ctaaaatgtc tatcaaaaga gcaattttgg
    14221 gtaaaccctg actgtggctt aaaaacacgt cgtgaagcag aaacaattgc tgccttggag
    14281 gttcttgttt cagctaccaa agaggttcgt cagcaattag ataattaata ggttaatcag
    14341 taaaggattg ccatgaggga ggagtaaaga agatgtccaa gtttttagag aaattaaaaa
    14401 cagatattct agtagctgac ggtgcaatgg gaactctcct ttatacttat gggctagata
    14461 cttgtcatga atcctacaat gtaacacatc cagaaaaggt tctagccatt catcaagctt
    14521 atattgaagc gggtgcagat gttattcaga cgaataccta cggggctcaa cgtcatcgat
    14581 taaagaacta cggtttggaa gatcaggttg tctctatcaa tcaagcagca gttaatctag
    14641 ctcatcaagc gactttgggt aaagaaacat ttattttagg tactgttgga ggttttcgtt
    14701 ctcaaagaca atgcgattta acattggata atattgtaga agaaactttg gaacaagttg
    14761 aggctttatt ggcgactgga cagctagacg gattattgtt tgaaacttat tatgatattg
    14821 aggagataac gacagtttta aaaattgtga gggagatgac tgatctacca attattacta
    14881 atatttctct tcatgaagca ggggtaacat caaatggaaa gcctattgta gaggcgctca
    14941 gccaattagt tatgttaggt gcagatgtga ttggcttaaa ttgtcacttt ggaccctatc
    15001 atatgatcca atcattgaag caagttcctt tatttgccca gtcttatttg tcagtttatc
    15061 ctaatgcaag tcagttatct ttagacggtg agaatagtca ataccagttt agtcagaatt
    15121 cagagtattt tggaaaatct gcagaacttc tcgtagcaga aggagttagg cttattggtg
    15181 gttgttgtgg gacaactcca gatcatatta gagctgtgaa acgatctatt agaggattga
    15241 aacctattga acgaaaggtt gttactccta taatacctgt aaaagatttt gttaggcgta
    15301 tcagacgaac agatactcta gtggataagg taaaaaaaga agtaacaatt atcgcagaat
    15361 tagatcctcc aaaacattta gatatcgttc agtttcaaaa ggctatacga gcgattgacc
    15421 aaaaaggtat tgcagcaatt actttagcag ataattcact atctaataca cgtatttgta
    15481 atttaagtat agctagcttg cttaaagatg aaattagtac acccttttta ctacatatag
    15541 cttgtcgtga ccataattta attggcttac agtctagact gttgggcatg gaattattag
    15601 gttttaacca tattttagcc ataacaggag atccgactaa acttggtgat ttcccaggag
    15661 cgactagtgt ttatgatgtg acttccttta aactcctttc tctgattaaa caacttaatc
    15721 aaggtttatc ttatagtggt gctagcttga gacgtccaac cgattttaca gttgctgctg
    15781 cctttaatcc taatgtgaaa aaccttactc gaaccgtaaa attgattgag aaaaaagtag
    15841 cttctggtgc tgattatttt atgacacaac caatatttga tcactctgtg ttaaaggaat
    15901 tagctgatct tactaaaaca gttgaacaac ccttttttat cggaattatg ccaataacaa
    15961 gttataataa cgctgttttt ctacacaatg aagttcctgg tattaaactt tctgagtcat
    16021 ttttatctgc tcttgaaaag gtaaaggacg acaaagaagc ttgtttaact ttggccttaa
    16081 acgagagtaa gtccttgatt gatgaagcct tgaactattt taatggtatc tatttgatta
    16141 ctcccttttt acgctatgat ctgactcttg agttaattga ctatatacaa aaaaagcaag
    16201 taagaaagtc ctcttaactt tcttactttt tagttggtcg tttattatta taataaaagt
    16261 tacgtaccaa aacagaggtt aaactagatt tttaagccac cttctatact aagataaatt
    16321 agtttcttta agttttcttt aagtttatag acttagaata aagcgttatt ttgatgggta
    16381 gcataaaatc aaggcataat atagatgtca gcgatttttt tagactaacg aaataacgat
    16441 gtaaagctta ataggggtgt atcaaatact cacttatata gtaccaaaag cctatagcct
    16501 gttggctagc ttgaccgtat tcaaatattg aataaatctt ggttgagaaa gggaggtata
    16561 aaaatggtat gtgtcgattt tattgatcaa aaagtttata gtgaagcaga aggtctattt
    16621 gtttccgatt tagacttagg cattaaaggt ttaataagag cacgttatcc ttttgctctg
    16681 gatagtgact ttatttcgta tggaaattta agtcattatt gtatgaatta tcttgacgaa
    16741 attgttcagc gagcgaatca gaaaaatgag aagataaaat ataatgtgac taccttgatg
    16801 aaagaagaag agaagccctt caacgttgaa gagcgtttga acaagcaagc aactatcggt
    16861 caacgaattg ctgatgatgt tgcgcgtttt ggcggatctt ggacttttat tatcgttttt
    16921 gtatccatta tggctatttg gatgttggtt aatatcatga agccttttgg tattcaattt
    16981 gatccttacc catttatcct gttaaactta gctttatcaa cgattgctgc aatccaagca
    17041 ccacttatta tgatgagtca aaatcgtgct gctgattatg atcgtttgca agctcgaaat
    17101 gactttaatg tcaataaaac ctctgagtta gaaattagat tattgcacga aaagattgac
    17161 catatggtac aacaagatca gtttgaattg ttagagattc aaaagttaca gacagaaatg
    17221 ctagtatcat tgggaaatca gttagcacaa ttgaaacaac tacaaaaata atcttttagc
    17281 agttatttaa aaatactgat acaatactaa gaatttaaat taatactgca acttcctata
    17341 atggagagca gtatttttac tttattaata acaaaaaccc cttagatgat gtgtctaagg
    17401 gatttttgta tcggaataat cgagttggtg aattattagg ctagtgtctg tccgaaagcg
    17461 tctgctgcta aaatagcttg agcaaacatt ttgggatcga aaggggtatt tttgtattca
    17521 ttagtagtca ttgcaatttc aacaattttt ggaatatctt tttctgataa agagacgtca
    17581 gctagagttg ttggcaaacc tagtgactta ttaaaacgag ctactttttc aagttcttct
    17641 agttcgttga aataagcatg taaaactaac acaccaaacg caactacaac gccatgtaag
    17701 aattcacctt gtctttgaat agcggttgta ccatagtaaa atgcatgcgc gtgacaagaa
    17761 ttgtagtaga aatcaggttg attaactaaa ttagaagcat agccagttga gattaagata
    17821 tcaagcgcaa tttcttcaac tgcacgcgaa gctaaattag cttcaacgtc ttttagacct
    17881 tgttcaccaa attgataaaa agcttccttt gaagacagag cgactgcttg tcctaacact
    17941 gcagtatgtg gtagtttatt ggtcttagcc tctaaggtag cacgttctac ttcaggggct
    18001 tttgaaatac cgtccccaat accagcccaa aaatatttgc ttggtgcctg tgcaatgatc
    18061 cgtgtattga taaaaatatg aagtggagac tcgggatagc cgtatcttaa gaaagaatgg
    18121 tcatcattat atacaactgc aatggcagta cctgcggagc aattagaaca aatagttggg
    18181 atagtaaagc tatttttacc taactctttt gctaccattt tagctgtgtc taaagctttt
    18241 ccaccgccaa aacctaagat agcatcagca gcttgaactt gtgggttggc tactaacttg
    18301 tcaatgttag attgtgttga atccgttcca tagacaaagc ttcctgtgac tttaatattt
    18361 gcttcctcta aaatagagcg aacctcggct tctgagctgt ctagggcacg tttgccaccg
    18421 acgaaagcaa tgctttgaat acggtaagtt gccaaaacgc taggaatttc tttgaagcaa
    18481 tcttctccgt agctataatt tgccattaca tttgttgtca ttcagtgtcc tcctctattc
    18541 caaaggtcga ttagagacag ctattttcta aaagtgaata gttttttgtc tcttgaccaa
    18601 gtgattatca tattatagca gaatattcag aaaattggaa catgaaaaac aatttaattt
    18661 tcaagaataa gaatagaaaa ggtaaagcga gatatacgag taaatactgt cagtataata
    18721 attataagct cttgagaggg tctgtattgg atgcaacagg gagttctttt aggtattgct
    18781 caagatcgcg ttggtgtttt agaatcttta ttttttgagg gtagttctca ataacttgct
    18841 tataatttga tttttgttta tcagagcgac cgtctttaag aatccaactg ataaattcaa
    18901 aatctaattt ttccgggcat ccattggcca tatcaggtcg agtcttacca cgaaacttac
    18961 agtaacgttt gaatgctctc cacaagcagt tgaagcgatt aaagtcaaaa aaaataatct
    19021 catctgcatc tgctagacgt tcttgatata gcagtttttt gtagttacct tctataatcc
    19081 atgttcttct ttctaacatg ctgctgacat cgtcaatcat gtcatcatat tttctctctt
    19141 cccaatttgg ggcgaagtga atactatcaa ggtgtaatac attgcaatta tagtaatttc
    19201 ccaattttct cgctaaagtg gacttacccg acccactata tccgattatt gctattttca
    19261 tatactacct ccgttaaatt gtttacttga agttaatgct gtacttagga tttacttatt
    19321 ttataagggt tattcacttt tttagattaa tatatttttg atataagtta tccatacaca
    19381 tggtaacata attaccagta aatgtaatct agatatcaat actatggttg acgatattgt
    19441 taacttttgg ttcaaaaaat tgggtaaaaa tcatcggagg gaactgccaa acaaactcac
    19501 agtttaaaat actaagtaat aaaaagtctt gaacttatta tgtttaaggc tttttaagcg
    19561 tctaaactac ttggtctagt atgaatttct aaacagaaag taaaaataat gagttttagt
    19621 atggtttttt gggcataatg gaacgatttt atctaaaata gtacgtttct tattaaattt
    19681 attttttaag aaaaacatta ggctaatcta tttgataaaa atagtgataa gtagaaattt
    19741 tctaaaatat ttagatttat ataaggtggt aaattctcgt tatggttttt tacatgaaaa
    19801 attaaattaa attatcagaa attaacaaaa ggataggcgc aagtatatta tagcaaaatg
    19861 ataatattag ttttatgaaa aaaatgcagt ttatttgtaa taatgtaaaa tataggggaa
    19921 aagaaagcgc ttgacgacct tttggacaag tagtaagata caaacatggg ccctgtaaat
    19981 taaaaatact gcagtaaaag tgattttagt ttaaaggagg aaattcatta tgaacgttaa
    20041 acatatgatg tatctatctg gaactctagt ggctggtgca ttgttatttt caccagctgt
    20101 attagaagta catgctgatc aagtgacaac tccacaagtg gtaaatcatg taaatagtaa
    20161 taatcaagcc cagcaaatgg ctcaaaagct tgatcaagat agcattcagt tgagaaatat
    20221 caaagataat gttcagggaa cagattatga aaaaccggtt aatgaggcta ttactagtgt
    20281 tgaaaaatta aagacttcat tgcgtgccaa ccctgagaca gtttatgatt tgaattctat
    20341 tggtagtcgt gtagaagcct taacagatgt gattgaagca atcacttttt caactcaaca
    20401 tttagcaaat aaggttagtc aagcaaatat tgatatggga tttgggataa ctaagctagt
    20461 tattcgcatt ttagatccat ttgcttcagt tgattcaatt aaagctcaag ttaacgatgt
    20521 aaaggcatta gaacaaaagg ttttaactta tcctgattta aaaccaactg atagagctac
    20581 catctataca aaatcaaaac ttgataagga aatctggaat acacgcttta ctagagataa
    20641 aaaagtactt aacgtcaaag aatttaaagt ttacaatact ttaaataaag caatcacaca
    20701 tgctgttgga gttcagttga atccaaatgt tacggtacaa caagttgatc aagagattgt
    20761 aacattacaa gcagcacttc aaacagcatt aaaataatat ttgcattttt cgtgtgatgc
    20821 tgtcgacttc gtgattttgt actaccatga tttttacgat taaaaggttt acgataatag
    20881 tcataatagt agaacgatgt caccatttta aataataaag tgattagtca tttgactaaa
    20941 tttaccaagt atcaaaggaa ataaagatta tgactaaaaa gataactgtt gtagcattag
    21001 aaacattgat tgcccagcat aataatatcc atttgataga cgttcgtgaa gagcatgagt
    21061 atcgtggggg gcatattcca ggtgcgataa atcttccttt gagtcaactc agtcataagt
    21121 ttgaacagtt agataaaaat aaggaatatt atcttgtttg tcaacgaggg ggaagatcta
    21181 ttagagcatg ccaatttctt gagttgaaag gctacaaagt aatcaatgta gatggtggca
    21241 cagaagcgtg gagaggaaat ctagaagtgt aaatatgtcc tgtgtagtct accatatttg
    21301 ataataaaaa aattgacatt atctcactat taatgacctt ttagtaaaca taaagaaaag
    21361 tatgtgtttt gaaaaggagg atgattatcc ttttcaaaac catacttttt gtgtcttgtg
    21421 gcttggtctt aattaatagt tgttaggaca atgtcgtaaa tgacaaggag aataaaaggc
    21481 caactagtaa gtaataattt tgtgtattgt ttagagttta ttgagtcagc ctctcgaacg
    21541 ttagagtgca gacttatacg atggcgagca gtattcctat gcgtaaaaag gtaatataat
    21601 ataaataaga ttgctaaaat tagcaatata ttttcaaaat aagtcccgta taactcgttt
    21661 actgttgata gctgggtgaa aatttctacg ataaagttat tgcagatatg aaggagtagg
    21721 gctaccttaa aggaataatg gtaagcgata aaacccatta tgatacccgc aatagatgta
    21781 gtcaatagct gagaaatgtc ttggtgcatt aaaccaaata ggatgccaga agtaataatg
    21841 gcaaaccatt taccatactt ggataataaa ttcaatacta ttcctcggta taaaacttct
    21901 tcaaaaaaag gacctacaac aacagaataa ctaatcataa gtatactgaa ggtggcatca
    21961 taattttgac tttccgattc cataatattg agatgattta gcacagcatc ccatattgtg
    22021 gagactatat tattagtagt atatataatg cttaacaaga ataaaatagt ggtgaaagtg
    22081 attgtatgat gagttgccgt tagatgtttt tttaaatttt taaaacccat aaaaaaacaa
    22141 ggaatgagta aggcgattaa agtacttgct atacctatcc agacatcatt ttgtaagatt
    22201 tcagcaaata gtgtagtcgc caaacttaaa acaatagcct gagctaaaag tatcagcccg
    22261 attaggttaa gtctcttttt ttcttgattc aattgattac ctccttatta ataacggtaa
    22321 cgacaaagaa atattataat gtataatgta taggtttcta agtcattagt caatagggac
    22381 gagcacttgg atacttctaa taagataaaa tacatagaca agactatggt tttggcttct
    22441 ctaaagatta cctaaaaatt tataagttct cgagcagaaa ggatgagttt ggatattgag
    22501 aggatgggct tgaccagctg aaagaaaaat gaaccaaggg taatcgcttc gaaaaaagaa
    22561 ggaaatttca attagaaact tccttttttt atcaatctag tattggcgcg tgctagggac
    22621 tttataattc gttataaaat cttcgatttc agcaatatca tctgagaata ggacattttc
    22681 tctatcttcc agactaagaa aaccctcacc aaccatatga tcaaacatat tttttaaatc
    22741 attaaagtag ccattaacat tatataaaat gcaaggatta tcattttgac caatgcgaga
    22801 ccaagagata acttcggaaa tttcttcaag agtaccaggt ccaccaggta gagcgataaa
    22861 ggcatctcca agtagcatca tttttgcctt acgttcaggc atattattta ctatgataag
    22921 ctctgataaa ttttcatgtg caatttcacg atctcttaaa aatgtcggaa taacaccagt
    22981 agtatagcca ccatttgcaa tcacagtatc agccatgact cccattaatc caacttttcc
    23041 tccaccatac accagagaat gtttgttttg tgccatccat tgtgccaagg aaactgtttt
    23101 ttcactataa acaggatttt ccccagtact tgctccacaa aaaatagtta ttctcatgtt
    23161 tcaaacctct tttctaattt agtgcgatgt gcgctgataa tctcttctag tgagatatta
    23221 tactgatcag caagtatgaa gatattatct aagacatctc ctagttcttc ttttatatca
    23281 tttaagtttt ccaaatcagt ttgttcggtt tcgtcaggtc tgtcacgtcc aatttcatat
    23341 ttgcgaatgg cttgagccaa ttcgccgact tcttctgaaa gaaagttact tctaataaaa
    23401 gaattgtatt gataccagtt tcttttttta tagaattcgc tcacccattt ttgataatct
    23461 gtaatatcca ttgttccctc cgtttcaagt atattatagc aatattgctt tattaatgaa
    23521 aatttgaatg ctaattaata ggaaaagttc gtatttggta tttcttctag tttgtgaata
    23581 gtcatattta aacctaaaag gttagcgcgt gctttaagca ttttttaaat attttaaacc
    23641 ataatgtatg aagatttatc tctttattaa agtgaggtta tgtagtgaac aagaaatcct
    23701 ttttccagtg tgttatacta tttgtttctt ttttagtgtt aacattagca gttgaatctc
    23761 aaagtgatat gataagttat ttggataata tcacttcagc tttttttcaa agcatacgaa
    23821 accccgattt aactaactta atgacgataa tatcaacagt tgtcagccct ttgacaacgt
    23881 cattaatagc acttgttatc cttgggtatc aatatttttt aaaccaaaga atagctgtgt
    23941 ggctatttat gttatttttt ggtaccaatg ctttagcatt gcttttaaaa gatatcatcg
    24001 cacgtcatag acctatgaat caacttattt ttgactcggg ttatagtttt cccagtggac
    24061 atacgatatc aacattttta ttaatgattc ttgttttagt ggttgcgaga cagagattga
    24121 gaagagtatt gtcacaagta gttttcgtaa tatttgcatt agtgatcctt gcatcagtta
    24181 ttttttcaag gctctattta gaaaatcatt ttttaacgga tattttaggg tcgttgttac
    24241 taggagcatc gtcatattgt ggtttgtctg ctattgtatc tttgaaagag ttacaataaa
    24301 aatagtagtt accaatgaat tataaaaaag cctttcttaa gggctctttt ataattcatt
    24361 atttttcaat gacaaatatt agtgaagtta atttttattt aaccattgtt attattaatg
    24421 ctgttttgcc acttattaat caacgaaaaa gtttcttcta gggaaacttt tttatattat
    24481 atttcctgat aggaactttt atttttgatt tagaaactct ataatggacg tgaaaggaga
    24541 ttgaccgatg cttggagaga atatctattt acaacgtact cagattggta tgacgcaaga
    24601 gaatttgagt gattatctgc atttaacaaa aactacaatt tcaaagtggg aaaataatca
    24661 agctaaaccg gatattgact atcttatttt gatggctaat ctttttgata ttagcttgga
    24721 tgacttagtc ggttatcaaa aaacattgtc agatgatcaa agaaatcagc tcattaagga
    24781 tctaaaaata aaagcaaatg tattgtctga gagagatttc tttcaggaag ttaaggaatt
    24841 atcaaaacag ttccctaacg attttaagac tctgttgata atgattaata tggtcttgag
    24901 caacctaacc aaccttaatg attcagaaat gaaagagtgg tcactaagta cactaaataa
    24961 aattattagt aaaacagtag ttgagtctga cctccagacg gcgataatgt tcaaaacggt
    25021 cgttcttttt cacaaccttg aatatgatga agtgataaac ctttaccaga atagtcccta
    25081 taaattaggt gaagaattac ttttagctaa tagctttttt gcaaaaggcg atgtttaccg
    25141 agctaagcaa gtattacagg ttgaaattta tcagcagatt ctacttgtat gcgagtatct
    25201 attgagttta tcaacttttg aagttgaaga aaacaaaggc gatatttttg aaaggcttga
    25261 acatatgata gaagcgtttc aattaaactc tttgcatccc aacacagtca tcaagtgtta
    25321 ttatagttta gcttgtcatt atagtaaaag tgatgatgat aaggcattag gatacttaaa
    25381 acaatgtttt aaatctttaa aacaattgct ccaaagattt gaattacacg gtgatacttt
    25441 cttttatacc atcgatgatt ggttagagac aatacctact ggtattgcac caccgacgag
    25501 tcaattacag gtaatagaac gagttgagga cttatttcaa aattcacaat ttaaagaact
    25561 atcagggcgt aaagaatttc aacaaatgat tcaaaaactt aacgatttaa aaaaggatta
    25621 ctaatatgaa tacagaaata ccaaatcaat tattaccatt cctagttccg ttgattatgt
    25681 tgcaaggagc tttaattatc atttctctag tcaaactaag taaacttaag atgactaaac
    25741 acctttcaaa gccagtttgg tttttggtca ttatatttct taatataatt gggtcaattg
    25801 catttcttat tttggaaggg aataatgaat gaaagctatc tcatttcaaa acgttacaaa
    25861 atcttttggt cctaaaaaaa tattaaataa tgtttctttt gatttagaag aaaatatgat
    25921 ttatggattc gtaggaccaa atggagcagg taaaacaacg acaataaaaa tgattttagg
    25981 attgttaaaa ttcgatacgg gtttcataac aatttttggt aaaaaagtta attttggaag
    26041 aacagatacc aatcaactga ttggctatct cccagatgtt cccgagtact acgattatat
    26101 gacagctcta gaatatcttg atttatgttc tggtttagcg agatcaaagc ataagctttc
    26161 taacaaagaa ctacttaggt cagttggact tgacgataat caccaaaaga ttgctactta
    26221 ttctcgagga atgaaacagc gtcttggatt agcccaagca ctggtccatg atcctaaaat
    26281 tattatttgc gatgaaccaa cctctgcatt agacccgaaa ggtcgccaag atattttaga
    26341 tattatttca aatcttagag gagaaaaaac tgtgattttt tctacccata ttctctctga
    26401 tgtcgaaaaa atatgtgatc acgttctggt gttaactaag tgtgggattt attctcttga
    26461 agagttaaaa ggaaaaaaat cagaggaaaa ttattcggtt cgtattttaa taaaagtgac
    26521 aaagtctgaa gctaaggtac tatctcataa ctatcagata gaaaagaaag ataatgagta
    26581 tgcactgact ctcaaaggct ctaaaatgga taacaaagca gatttactag ctggttttta
    26641 ccaagattta gtttctttaa agattagccc atcagctatt gaagtcattg acaatagttt
    26701 agaggagctt tatttggagg ttaccaaatg aaatctttag cagtaatgtt aaaaaaagaa
    26761 tggatggaga atgttaggac atacaaagtt atatctattc tgattacttg ttcaatattt
    26821 ggtatattag gtcccttaac tgcacttatg atgcctgata ttatggcagg aattttacct
    26881 aaaaaactac agggagcgat accagagcct acctatattg attcttatat tcagtatttt
    26941 aaaaatatga atcagcttgg tttagttatt t
//
LOCUS       DATRSD010000017        26590 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRSD010000017 DATRSD010000000
VERSION     DATRSD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26590
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.017"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            41..523
                     /locus_tag="VBD85_000593"
     CDS             41..523
                     /locus_tag="VBD85_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133163.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(594..1703)
                     /gene="dinB"
                     /locus_tag="VBD85_000594"
     CDS             complement(594..1703)
                     /gene="dinB"
                     /locus_tag="VBD85_000594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO7133164.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTLILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPDRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            1873..4185
                     /gene="pflB"
                     /locus_tag="VBD85_000595"
     CDS             1873..4185
                     /gene="pflB"
                     /locus_tag="VBD85_000595"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO7133165.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            4286..4660
                     /locus_tag="VBD85_000596"
     CDS             4286..4660
                     /locus_tag="VBD85_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7133166.1"
                     /translation="MLNHKFLQVLKYEGVVSITSWIELAPHVTNTWNSYLTITDDQRI
                     LAPAAGMTHLENDLNNNSKIIMTLGSREVEGRDGYQGTGFRIEGTAKLLEAGSDFEIV
                     KEKYPFLRKVLEVTPINVIQLL"
     gene            complement(4695..5624)
                     /locus_tag="VBD85_000597"
     CDS             complement(4695..5624)
                     /locus_tag="VBD85_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO7133167.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(5621..6376)
                     /locus_tag="VBD85_000598"
     CDS             complement(5621..6376)
                     /locus_tag="VBD85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO7133168.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESLGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            6501..7397
                     /locus_tag="VBD85_000599"
     CDS             6501..7397
                     /locus_tag="VBD85_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO7133169.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIGFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(7476..8324)
                     /locus_tag="VBD85_000600"
     CDS             complement(7476..8324)
                     /locus_tag="VBD85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7133170.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAMFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(8493..8945)
                     /locus_tag="VBD85_000601"
     CDS             complement(8493..8945)
                     /locus_tag="VBD85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7133171.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFERLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(8963..10165)
                     /locus_tag="VBD85_000602"
     CDS             complement(8963..10165)
                     /locus_tag="VBD85_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7133172.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            10321..10980
                     /locus_tag="VBD85_000603"
     CDS             10321..10980
                     /locus_tag="VBD85_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7133173.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            11004..13289
                     /locus_tag="VBD85_000604"
     CDS             11004..13289
                     /locus_tag="VBD85_000604"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO7133174.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFVPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     DWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKIFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTIKFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIDPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            13293..13652
                     /locus_tag="VBD85_000605"
     CDS             13293..13652
                     /locus_tag="VBD85_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133175.1"
                     /translation="MKLLTKELFDDSQHFWYQINLSQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNHFWYFGNTTDTSILMIAHLNRKFYIQVNLKDFDFALNLITINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            13698..14678
                     /locus_tag="VBD85_000606"
     CDS             13698..14678
                     /locus_tag="VBD85_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7133176.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMTINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGVYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(14763..16511)
                     /gene="cydC"
                     /locus_tag="VBD85_000607"
     CDS             complement(14763..16511)
                     /gene="cydC"
                     /locus_tag="VBD85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO7133177.1"
                     /translation="MFKIPLFKELKTDQWVKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIGWLLYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFVFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(16504..18222)
                     /gene="cydD"
                     /locus_tag="VBD85_000608"
     CDS             complement(16504..18222)
                     /gene="cydD"
                     /locus_tag="VBD85_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO7133178.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYILLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESHLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDKGSLKVDG
                     REVVNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVIRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNNV"
     gene            complement(18222..19241)
                     /gene="cydB"
                     /locus_tag="VBD85_000609"
     CDS             complement(18222..19241)
                     /gene="cydB"
                     /locus_tag="VBD85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO7133179.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(19242..20669)
                     /locus_tag="VBD85_000610"
     CDS             complement(19242..20669)
                     /locus_tag="VBD85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO7133180.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(20772..21980)
                     /locus_tag="VBD85_000611"
     CDS             complement(20772..21980)
                     /locus_tag="VBD85_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7133181.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIRDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(21993..22892)
                     /locus_tag="VBD85_000612"
     CDS             complement(21993..22892)
                     /locus_tag="VBD85_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO7133182.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(23301..24224)
                     /locus_tag="VBD85_000613"
     CDS             complement(23301..24224)
                     /locus_tag="VBD85_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133183.1"
                     /translation="MKKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDAELYERAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAAT
                     PQVSSPKAERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYQQQNAAVTPQVSS
                     PKAERQLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSTPKSER
                     QLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSSPKAERQLTDS
                     EIYERAQKQVLPKYIQGSLSGILNQHSTLNV"
     gene            24496..24630
                     /locus_tag="VBD85_000614"
                     /pseudo
     CDS             24496..24630
                     /locus_tag="VBD85_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            24643..26223
                     /locus_tag="VBD85_000615"
     CDS             24643..26223
                     /locus_tag="VBD85_000615"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEO7133184.1"
                     /translation="MAEKITSLRQALWNSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEEHFNTFTDTQKIFEDAYQDEDLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFKDIDLYSKKLGSTPQKQNQTIANVMKT
                     LNEIDFESVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFSGREDQK
                     GMTLYDPAMESGTLLLNAKKYSHQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDAHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLNDIDQEIAKTNAKLDQLMK
                     RLVGTTKEAQDELDAFRK"
BASE COUNT         8930 a         5134 c         4356 g         8170 t
ORIGIN      
        1 taatactttt attaaggttt atttagaaaa aggagatatt atggtttcat atgaaaaagt
       61 ccgccgctct ctgcgcacag ctacaatcac tatcattgta ttaaatagtt tgtctttagt
      121 attcagattg tttacaggaa tttcagtgca gctagccaaa actgaaatta ataaagggaa
      181 cacaggtaat cttcctaaag aacatatcga agcagtcttg agcgccacaa caccatttat
      241 gctatttgtc actgctttaa tagtattagt caacattgcc attgttattt tctgtattaa
      301 aaatttaaga gcaattaaaa gaaatcaaat ggttaattac cttccttatt accttggctt
      361 cgccattact gtaggacttg ttattttggg ttttctaact actaaagcac catgggcaat
      421 agctataaat atcgtcttcc aagctatttt cggtcttctt tatttccatg cttatcaaaa
      481 agcacaaaaa ctcaacgaac gtgatcttga agagactaac taaaagtaaa agcactagaa
      541 tctagtgctt ttttcttttc aaagagagtt aaatatagtc atcgtcccaa cttttaaaaa
      601 tctaaataga tagcttcatg ttcattttcc agggtcgtta ctgtaacacc taagagtcgt
      661 atgccaaaaa ctgactcttc taaggtatca aatatcgctt tagcaacttg atcaatgatt
      721 tgaaaatctt gagtatactc ttctaatgtc atacgtttcg tcaatgtttc aaaatccgca
      781 tatctaactt ttaaaacgat tgtcttacca actttcttat ttttctccag agaagcaaca
      841 actctctgaa cgttctttga aatctcagct ttgatatctg cttcgttata tagtaatttc
      901 ccataggttt tctcacttcc tattgattta cgaactctat caggttttac cggggaagca
      961 ttaatccctc ttgccttacg ataaaggtca tacccgaaac gaccaaacat atcaattaaa
     1021 gaaatttcag ataaggaaag gaggtcctca ccagtataaa cccctaaagc atgtaatttt
     1081 tcgacggagc gtttaccaac accatgaaat ttctctattg gtaagggctt caagaaatct
     1141 tgagcttgat ctggtaggat gagggtaagt ccttttggtt tctcaaagtc agaagccaat
     1201 tttgccaaaa atttattgta ggaaataccc gctgaacagg tcaaatggac atcattccaa
     1261 atatcgtatt gtatcatttt agcaagttta acagctgatt ttattcccat tttattttca
     1321 gttacatcta aataagcttc gtctatactc atcggttcaa ccaaatccgt atacttttta
     1381 aatatatctc gtacttccat tcctacttgg cgatattttt ggtaatttcc cgaaataaag
     1441 atagcttgag ggcatcgttc atacgcttct tttgaagaca tagctgaatg aactccaaac
     1501 tttcgagcct cataattaca agtagaaaca acccctctgc cgccggtttt tctgggatca
     1561 gaaccaataa taacaggctt tcctttaaga gagggattgt cacgctcttc cacagatgca
     1621 aaaaaagcat ccatatcaat gtggataatt ttacgcgacg tatcatttat taaaggaaaa
     1681 attaacattg ttctctcccc cattctctct actccattat aacagattac aacttaatga
     1741 tacagattaa aaaaactgta ttataaaaga atcataattt tgtcaagaaa tatgcttgcg
     1801 aaaacgttta ctttttccta aaatggatat gtaaacaaaa ttaaatctgt cacaatgttt
     1861 aaggagaaaa ctatggcaac agttaaaaca aacacagata tctttgaaca agcttgggaa
     1921 ggctttaaag gtgttgattg gaaagaaaaa gcaagtattg ctcgcttcgt tcaagcaaac
     1981 tatgcacctt acgatggaga tgaaagcttc ttagctggag caactgaacg ttcacttcac
     2041 atcaaaaaag tgattgaaga aacaaaagct cactatgaag aaactcgctt cccaatggat
     2101 actcgtgttg cttcaatttc tgaattacca gctggtttta tcgacaaaga taacgagctt
     2161 atctttggta ttcaaaacga cgagcttttc aaattgaact tcatgcctaa aggtggtatc
     2221 cgtatggcgg aaactacact taaagaaaat ggttatgaac ctgatccagc tgttcacgaa
     2281 atcttcacta agtacgctac aacagtaaat gatggtatct tccgcgctta tacttctaac
     2341 attcgtcgtg ctcgccatgc acacactgtt actggtcttc cagacgctta ttcacgcgga
     2401 cgtatcattg gtgtatatgc tcgtctagct gtttacggtg ctgactactt gatgcaagaa
     2461 aaagttaatg actggaatgc attgaatgat attgatgaag agtcaattcg tcttcgtgaa
     2521 gaaatcaacc ttcaatacca agctcttggt gaagttgtaa aacttggtga cctttacggc
     2581 gtagatgttc gtaaaccagc catgaacact aaagaagcca ttcaatgggt gaacattgct
     2641 ttcatggctg tatgtcgtgt tatcaatggt gctgcaacat cacttggacg tgttcctatt
     2701 gtccttgata tctttgcaga acgtgacctc gctcgcggta cattcacaga atcagaaatc
     2761 caagaatttg ttgatgattt tgtccttaaa ctccgtactg taaaatttgc tcgtactaaa
     2821 gcttatgatg ccctttactc aggtgaccca acctttatca caacttcaat ggcaggtatg
     2881 ggagctgatg gacgtcatcg tgttaccaaa atggactatc gtttcttaaa tacacttgac
     2941 aatatcggta actcaccaga accaaactta actgttcttt ggtcagatca attaccatat
     3001 gctttccgtc gctactgtat gtcaatgagt cataaacact cttcaatcca atatgaaggt
     3061 gtatcaacaa tggctaaaga aggctacggt gaaatgtcat gtatttcatg ttgtgtatct
     3121 ccacttgatc cagaaaacga agacaaacgt cacaaccttc aatattttgg tgctcgtgtt
     3181 aacgttatga aagcacttct tactggtctt aacggtggtt atgatgatgt tcataaagat
     3241 tacaaagtat ttgacatcga cccaattcgt gacgaagtac tcaactttga cactgtaaaa
     3301 gctaactttg aaaaatctct tgactggttg actgatactt atgttgatgc gatgaatatc
     3361 atccactaca tgactgataa atacaactat gaagctgtac aaatggcatt cttaccttca
     3421 catgtacgtg ctaatatggg atttggtatc tgtggttttg caaatactgt cgattcatta
     3481 tctgcgatta aatacgctac tgttaaacca atccgtgacg aagatggtta catctacgac
     3541 tacgaaacag ttggtgactt cccacgttat ggtgaagatg atgatcgtgt tgactcaatc
     3601 gctgaatggt tacttgaagc cttccatgga cgccttgcta aacacaaact ttataaagat
     3661 gctgaagcaa ctgtttctct tcttacaatc acatcaaacg ttgcttactc taaacaaact
     3721 ggtaactcac cggtacataa aggagtatat ctcaatgaag atggtagtgt aaacctttct
     3781 aaagtagaat tcttctcacc aggtgctaac ccttctaaca aagctaaagg tggctggtta
     3841 caaaacttga attcactttc taaacttgat ttcgctcatg caaatgatgg tatttcattg
     3901 acaactcaag tttctccacg cgctcttggt aaaacatttg atgaacaagt tgataacttg
     3961 gtaacagtcc ttgatggtta ctttgaaaac ggtggtcaac acgttaactt gaatgtcatg
     4021 gaccttaaag atgtctatga caagattatg aatggtgaag atgttatcgt tcgtatctca
     4081 ggttactgtg tcaatactaa atatctaact cctgagcaaa aaactgagtt gacacaacgt
     4141 gtcttccatg aagtactttc aatggacgat gcattaacta actagtttgt attcgatgaa
     4201 gtaggtttga ttatatcaaa cctactttta tctatattat caatagtact aattacttct
     4261 ttatataacc tcaaggaggg gcctaatgtt aaatcacaaa tttcttcaag ttctaaaata
     4321 tgagggtgtc gtttcgataa caagctggat cgaattagct cctcacgtta caaacacttg
     4381 gaatagctac ctgactatta cagatgacca gcgtatatta gctcctgcag caggaatgac
     4441 tcatcttgaa aatgatctca ataataattc taaaattatt atgactctgg gcagccgtga
     4501 agtcgagggt cgtgatggtt atcaaggtac aggctttaga attgaaggaa ctgctaagct
     4561 attagaagca ggaagcgatt ttgaaattgt taaagaaaaa tacccttttc tgcgtaaagt
     4621 attggaagta actcccataa atgttatcca acttttataa aaaacttctc ctagaaaatt
     4681 ctaggagaag ttttttattc aaagtacgat gttatcgcct cttgtacatg tcgtcgtttc
     4741 ttaatccaaa gtggacggtc atcataggaa aaggttctgt ttgtcaaaaa aatagctgct
     4801 gcctgctttt gataatttag tgcaataaaa ggaccagtat aaccagtatg gtttatccaa
     4861 tccttatcta tatcccaagc taatgatctt gatttactca aactataatt attccacaaa
     4921 ggcttcgcaa aatcatcttt taaataatga atagaaaatc tttgtaaatc atcaatagtt
     4981 gaaaataaac ctgcagaacc agtatgcttt cctaatacct tagctttagg atcgtgtaca
     5041 ataccatcgt taataccggc tacagttgga acagcttcta ctctaggacc aaaagaggtt
     5101 tctttcatct gaaaaggagt gaaaatataa cgtttgaaaa gtttatccaa actatcccct
     5161 aatacctctt ctaacataaa accaagtaaa agaaaattaa tatcagtgta tttaaacgat
     5221 ttatcttcca aaaccttgat atgattaatg gcatctttca gctgagtagc attcaactga
     5281 tcacgatttg gaataaaagg gtcaattcca ctgctatgtg tagctaattg ccttacagtt
     5341 aacgtctgat gatgaaacgt agggtaataa taccttaatg gcttatcaat atcaatagtc
     5401 ccttgataaa ctaactttgc tagtaaagtt cccacgccga caacttttga tacactggct
     5461 agatcataga ccaatcccgc tttaactttc tcattgccat caattgtccc caagtaatgt
     5521 tcgtgccact ctccattttc ataaatagct aagcttgcac catgataaag attatctttg
     5581 atttgctcct taatgagttc taaaacagta ttagttttaa tcattctttt gaaatccaaa
     5641 tctcaatatt actagtgtca gagatgacta aaatcttttc tttagaatca aggtaaacat
     5701 caagtcccaa tgattcaaaa gttgatttca agtcatttaa attagtatct tcattaacac
     5761 agcattctag tatctcaata tcccaagttt catttggagc aatttgcaaa tcttgtccct
     5821 gtgcttcttt gaaagataaa ttaattggga acttcccagc aaatacttta tcgtaaaaag
     5881 cttcagcttt cacggcatca ggtacattca aactaataga ttggaaagca aagtttgata
     5941 gacctgtagt gtcatctttt ttttctaact ctgggcgctc aattgccact aattgtgaca
     6001 aatcatcttc agcatgaagt aagaaaaggt ctccctcggg cgaaactgtt tcataggcat
     6061 aaccattttg accttgatat acttgaatgg cttgcgcacc atttgccaaa agcttttcaa
     6121 tatcttttgc atcttgagac ttaacaataa tctttgctaa cttcttagta ccattcaccg
     6181 cacgagtacg ataagttggg gattcctcaa taataaagct agcctctttg ttctgccatg
     6241 ctgagaaaac tgcgattgca ttttcttcac ttatcaattt aaatccaaga gactcctgat
     6301 aaaatgcaat attcaagtct ctattattaa cacgtaatac tgggtgttta aatgtcaatg
     6361 aatgaaataa cgtcataaat tcctccttgt tcagtctagt ctattttaga taattttaga
     6421 taatttagca agaatctttg tgctctcaat accataaagt caaattgaca gtttcactat
     6481 ttttttgtag actggatatt atgaatgaaa cactactatt acatgggatt cagcttattc
     6541 tgattatcgc catgattatc actttttatc agattgttag acatattagg tctcaaaaaa
     6601 taaatccttt caaacgattt ttcactggtt tgtggatcgg ttttgtaact gatgctctcg
     6661 atactcttgg tatcggctct tttgcaacaa cgaccacatt ttttaaacta actaagcttg
     6721 ttgaagacga tcgtaagata cccgcaacaa tgacagctgc acacgtactt ccagttttgt
     6781 tgcagtcact ctgttttatt tttgttgtaa aagtagaggc tttaacacta ataacaatgg
     6841 ctggtgcagc ttttattggg gcttttgttg gagctaaaat gactaaaaat tggcacgctc
     6901 ctactgttca acgtattcta ggaacactgc tcatcacagc tgctattatt atgctctatc
     6961 gtatgatcac taaccctggc gctggtattt cagattcagt gcatggtcta cacggcattt
     7021 ggttatttgt tggtatcggc ttcaatttta ttattggtgt attaatgact atgggactag
     7081 gtaattatgc tcccgaactt atcttcttct ccctaatggg actgagtcct gcagttgcta
     7141 tgcctgtcat gatgctagat gcagctatga taatgacagc ttctagtaca caatttatca
     7201 agagtggtcg tgttaattgg aacggctttg ccggtctggt tactggggga atattaggcg
     7261 ttattgtcgc tgttctcttc ttaactaacc ttgatcttaa cagcttgaaa acactggttg
     7321 taggtattgt tttatttact ggagcaatgt taatacgttc ttcctttaaa aaaactatgc
     7381 ccaaaagtca tcattaaacg aaatcagcta gcaagactgg ctgattttta tatttaaaac
     7441 aaaaaaagac cggtaaaccg atctttcttt aactattaaa gatataatag tttgaacaag
     7501 gctacagcga gtatacttgc tacgattggt gctaaaactg gcacccatgc ataccaccat
     7561 tttgaatctt ctttagcttg tccaagaatt tgttttggta ataagcggtg aacaatacgt
     7621 ggaccaaggt cacgcgcagg gttcaatgca ggtccagtcg gtccacctaa agaagcaact
     7681 agagtcatta ccaagaaacc aatacctaag tgagctacag caagtgaacc agttacgtat
     7741 ggtgaaatcc ttgtcgctgc tgtcgtttgg tcataaccag cttgaacaag tttaccaaca
     7801 agttctacac caaaatagtt cttcgttaaa gccaaagctc caaagaaaag aacaaaagaa
     7861 ccaacgaatt catttaagaa accattaatg taagccgctt tacgacttga tttctgtcca
     7921 tcatctaatg cagaaatagt tgaaaatgat ccaagaacat gatttggatt ttctgtctta
     7981 acaaagtaag gttgataaac cataacgaca actaactgtc caaacattgc tcccaaaatt
     8041 tgtgcaagaa tgtattgtcc aacgtgtgcc caagggaaaa gacctgaaaa tgccaatcca
     8101 agtgtgaaag ctgggttaat atggtttcca gagacattac caaacataag cgctggcatc
     8161 ataacaccta aaccataacc aattgcaata ataatccaac ctgaattatt tccttttgtg
     8221 ccttttaagt caacgttagc tacagcccca tttcctaaga tgataagaaa agcggttgcg
     8281 ataaattctg tgatatactt cacagtccaa gtgatttcga tcaatccttg tttcctccca
     8341 aaaaaatatt tacaacatta accattctac caagtaagct taatattgta aagcttatca
     8401 caaaataaaa ttaataaaat ttttaaccaa atatatttga taaaaaaata gagtactagg
     8461 ttcggactac ctagtactct attttattta gtttataagg ttttcttact atcacgaacg
     8521 actagcatat caactttcga atgacgtaaa atgtattctg aagttgagcc gatcaatagt
     8581 ctttcaaaag tgtttaatcc tgttgcgcct accataatta aatctgcttt ttcccttatg
     8641 gggatatcct ttgccaaaag agtcttagga tttccgattt cagtaatttt tttgatcttt
     8701 gtaaggcctt ttgaatgtgc taatttctca aaataggcta gcacatctgc tgactcttgt
     8761 tcttgcctat caaaaacata gtcactaaag actccctcac tttggtaagc gtgagcatct
     8821 ataacatgcg ttagcagtaa ttcagcatca ttacgaagag caacatttat ccctttttct
     8881 accgccaatt ctgactcgta agacccatcg atagcaataa gaatacgctc atatttttgt
     8941 gtcatagctt aattcctccc ttttatatag ttttagtttc aacttgtcta gatgatttta
     9001 agaaatatag aataccacaa acaacaggaa taatcgctgc tatccagaat aattctctaa
     9061 aggattgcac accagctcca agaaaaccat ctttaacaag tcccaaaatg taaggtccag
     9121 cacctaaccc taaatcaaga cctatcatgt aagttgacat ggctgtattg aaacgatgtt
     9181 cctcaacacc tttgattgat gctgcttggc cacaagacat aaaggtacca taaccaaaac
     9241 caatcaaagc acctgataga aggtaggtaa cacttcccat agcactccct aataaagcaa
     9301 gtcccaaagt taagaacaga taacttggat aaagcaccca cttatctccc ttagcgtcca
     9361 ttaatcttcc catagatggt ctggttaaag tgatgacaag tgcataaaca ataaagaaat
     9421 aagctcctac tgtcatcaaa ttaatttctg ttgtatataa tttttggaaa cctaacacgg
     9481 aagcatagga gatacccatc aaaaatgcaa taattgtgat aaaaattgct tttttctcaa
     9541 tgaaactatc aatagtccat gattttgatt tagctaactg ttctggattt aaagtaatat
     9601 ttttgactgg gaaaacaaat gctcccaaca ctacaatcgc aattaaaata ctacataata
     9661 caataaccat tttaaagtta atatgaaggt tgtctagcat aaatgttcct acaaaaggac
     9721 caatagctgc ggctaaactt gttgatagac cgtaaaagtt aatcccctca cctcttttat
     9781 cagctggtat ataagctgtt acaatagtat tagttgctgt tgacacgacg ccataaccaa
     9841 aaccatttag gaaacgaact aaatacatta ctccgatact tggcatataa aaataagcta
     9901 aagttgttag taagtaaaaa atagcccctc cacgtaaaac taacttacgt cctagaactt
     9961 ctaattgctt gccaaatata agacgagcaa tcaaagtccc tacaatataa atccccgtcg
    10021 ctaatcctgc ttggctagtg ctaacaccta actctttagt cgcaataaaa gctatgataa
    10081 cggtaaacaa ataatagacc atataaacaa taaagtttaa gatagtaatg cctataaaat
    10141 gtttgttgaa taatttgtct tccaatgcaa atggctcctt ttttcaattt cattttcata
    10201 aacattttac aaaaatatta gaagatacga tatttcaatg gtataatata agtgctagta
    10261 tcagactgca ttaacaaatg ttaaggtggt ctatcaaacc taaaacattt ggaggaagtc
    10321 atgaatcatc aaattttaca aaattacatt aatagtcata atcttccaat tatcgaaaaa
    10381 gactaccata aataccttac atttgaaagt ttagaagaag actatactta tatcctaaaa
    10441 gatggtattg ttaaacaaag tgttctctct aaatatggta ctgagttcaa tctgcgttat
    10501 gtgacaggct tagaaatcac ttctatccta aatacagact actcccaaca catgggagaa
    10561 ccttacaacg ttcgaatcga atccgaaact gcccactttt acaaagtacg tcgctctacc
    10621 ttcctaaaag atatcaataa tgatattgaa ctgcaaggat atgtgaaaga tttctatcat
    10681 aaccgccttg aaaaatctat gaaaaaaatg caatgtatgc tcactaatgg tcgaattggt
    10741 gctatctcca cacaattata tgacctcagt aagatgtttg gtgaagaacg agataacggt
    10801 gatatctata ttaattttgt tatcacgaac gaagaacttg gtaaattttg cggtatatcc
    10861 acaggaagta gcgtgagccg cattttgaaa caactaaaag acgatcatat tattcgtatt
    10921 gaaaaacaac atatcattat tacaaatgtt gagaaattaa aagatcatat tgtattttaa
    10981 tataacaata gaaaggatat attatgcgtt ataatcaatt cagctacatt ccaactaaac
    11041 ctaacgaagc ttttgaagag ctaaaaggac taggttttcc attaaacaaa aagaattctg
    11101 ataaagctaa tttggaagct tttctcagac attctttttt aaatcaaact gatactgatt
    11161 acgctcttag tctccttatc gttgatgcaa aaaccgatgc tctaaccttt tttaaatcaa
    11221 atagtgactt aacactagaa aatttacaat ggatttattt acagttatta ggctttgtcc
    11281 cctttgtaga ctttaaagac cctaaagctt tcttacaaga tattaacttc ccagtctcat
    11341 atgacaatat ttttcaaagt ctacatcact tactcgcctg tcgtggaaaa tctggcaata
    11401 cattaatcga ccaattagtt gctgatggtt tacttcatgc agataatcac taccattttt
    11461 tcaatgggaa gtctctggcc actttcaata ctaaccaatt gattcgcgaa gttgtctatg
    11521 ttgaaacatc cttagatact atgtctagtg gtgaacatga tttagtaaaa gttaacatta
    11581 tcagacccac taccgagcat actatcccca cgatgatgac agctagcccc tatcatcaag
    11641 gtatcaatga tcctgccgca gaccaaaaaa cataccaaat ggagggtgcg ctagcagtta
    11701 aacagcctaa acacatacaa gttgacacaa aaccatttaa agaagaagta aaacatcctt
    11761 caaaattacc catcagccct gcaactgaaa gcttcacaca cattgacagt tatagtctca
    11821 atgactattt tctttctcgt ggttttgcta atatatacgt ttcaggtgtg ggtactgctg
    11881 gctctacggg tttcatgacc agtggggatt accaacaaat acaaagcttt aaagcagtca
    11941 ttgattggtt aaatggtaag gttactgcat tcacaagtca taaacgagat aaacaagtca
    12001 aggctgattg gtcaaatggc cttgtagcaa ccacaggtaa atcttatctc ggtaccatgt
    12061 caactggttt agcaacaact ggcgttgagg ggctgaaagt cattatcgct gaagccgcaa
    12121 tctccacatg gtatgattat tatcgagaaa atgggcttgt gtgtagtcca ggcggctacc
    12181 ccggtgaaga tttagacgtt ttaacagaat taacatactc acgaaacctc ttagctggtg
    12241 attacatcaa aaacaacgat tgctatcaag cattgttaaa tgaacaatca aaagcaattg
    12301 accgtcaaag tggggattac aaccaatact ggcatgaccg taattaccta actcacgtca
    12361 ataatgtcaa aagtcgagta gtttacactc atggactaca ggattggaat gttaagccaa
    12421 gacatgtcta caaaattttc aatgcattgc ctcaaaccat caaaaaacac ctttttttac
    12481 atcaaggtca acatgtgtat atgcataatt ggcagtcgat tgattttcgt gaaagcatga
    12541 atgccttact aagccaagaa ctacttggca ttgacaatca tttccaatta gaagaggtca
    12601 tttggcaaga taatactact gagcaaactt ggcaagtttt agatgctttc ggaggaaacc
    12661 atcaagagca aattggctta ggtgatagta aaaaacttat tgataaccat tatgacaaag
    12721 aagcctttga tacttattgt aaagacttca atgtgttcaa aaatgatctt ttcaagggaa
    12781 ataataaaac caatcaaatc actattaatc ttcctctaaa gaaaaattat ctcctgaatg
    12841 gacagtgcaa actccatcta cgtgttaaaa ctagtgacaa aaaggccatt ttatcagccc
    12901 aaatcttaga ctatggtcct aaaaaacgat tcaaagatac accaaccatc aaattcttaa
    12961 acagccttga taatggtaaa aattttgcca gagaagcttt acgtgaactc ccgtttacta
    13021 aagatcatta tcgtgtcatc agtaaaggtg tcttgaacct tcaaaatcgt acagacttac
    13081 ttacaattga ggctatcgat ccagaacaat ggtttgatat cgagtttagc ctccaaccaa
    13141 gtatatatca attgagtaaa ggtgataatc taaggattat cctttataca actgattttg
    13201 aacataccat tcgagataat gctagttact ctataacagt agatttgagt caatcttatt
    13261 taactatccc aactaatcaa ggaaattaac ttatgaaact tctcactaaa gaactgtttg
    13321 atgattctca acacttttgg taccagatca atttatcaca agagagtaac ttcggagcag
    13381 tttttgacca tgataataaa aacattccac aggttgttgc aactattgtt gatgatttac
    13441 aaggttccgg aagttcgaat catttctggt attttggcaa tactactgat acttccatcc
    13501 ttatgattgc tcatttaaat cgaaaattct atattcaggt taatttaaag gactttgact
    13561 ttgcactcaa tttaataact ataaataatt ggaagagtct cctccaaact caactcgaag
    13621 ctctaaacga taccctagca atatttcaat aaataaggta gaatggagtg acaaagcaac
    13681 gcgagggaga ctgattaatg tcatcttatt ggaataacta tcctgaactt aaaaaaaata
    13741 ttgatgaaac caatcaacta attcaagaaa gaatacaggt cagaaataaa gatattgaag
    13801 cggcgctaag ccaactcaca gctgcgggag gaaaacagct cagaccagca ttcttttacc
    13861 ttttttctca acttggtaat aaggagaatc aagatactca gcaactaaag aaaatcgctg
    13921 cttctttaga aatccttcac gttgctacat taatccatga tgatgtcatt gatgactcac
    13981 cactaagacg tggaaatatg accattcaaa gcaagtttgg caaagacatc gcagtttata
    14041 ctggggattt acttttcaca gtctttttcg atcttatttt agaatctatg gctgatacac
    14101 catttatgac gattaatgca aaatctatgc gtaaaattct catgggagaa ttggaccaga
    14161 tgcaccttcg ttacaatcaa caacaaggta tccatcacta tttacgtgcg atttcaggta
    14221 agacagccga actctttaaa ttagctagca aagaaggagc ttactttggt ggtgcagaga
    14281 aggaggttgt tcgtctagca ggccatatcg gctttaatat tggtatgaca ttccaaattt
    14341 tggatgatat tctggattat actgcagata aaaaaacatt taacaagcct gtcttagaag
    14401 atttagcaca aggcgtttac agccttcctc tacttcttgc cattgaagaa aatcctgata
    14461 ttttcaaacc tattttagat aaaaaaacag atatggctac tgaagacatg gaaaaaattg
    14521 cttatctcgt cgtttcccat agaggtgttg acaaagctcg ccatctagct cgtaaattta
    14581 ctgaaaaagc tattagtgac ataaataagc taccccagag ctctgcaaaa aaacagttgc
    14641 tacaattaac taattacctt ttaaaacgca aaatttaaat aataaaaaaa cattccacaa
    14701 tgctagaaaa gcagttaggg aatgtttttt tactatcatt tatttatcgc acctatcaat
    14761 tatcatagat caccatcatc agcggctttc agctgacggt aacgttggct actttgagac
    14821 aattcttgag gagaaccttc caactctaat tgcccatttt ctataaataa gatacgatca
    14881 gcatgttcaa taccttttaa gtgatgtgta atccaaacta aggtcttacc ttccaattct
    14941 ttcataaata cccttagtaa ggcttgttca gtaataggat caagtccaac agttggctca
    15001 tctaagataa caattgggac atcttttagt aagattctag ccaaagcaat tctatgcctt
    15061 tcgccacctg aaaacctaag tccagcttca tcaaccattg tatagagacc atctgataaa
    15121 tcagtgacca tctctttcaa tccaactcgt tcaagaactt tccatacatc ttcttcacta
    15181 gcatcttggt ttccaatgcg aatgttattt agcagggttg tattaaaaag gtagggcgct
    15241 tgttgtatca ctccaatata gttagaaatg caatcaccaa ctattgaaac atcagcaccg
    15301 cctagggtaa tctccccttg acttgctttc aagtcgccac gaagtagact agctaaggta
    15361 ctcttgccag aaccactacg ccctaaaata gcaatttttt ctccttcttt aatatccaaa
    15421 tctaaatgat gcaaaaccca tttctcttgt ggcttatact ggaaacttaa attcttgacg
    15481 gaaaaatcat atggcttatt aggcaattga ttttgagttg tttcaaaata cgtttctgat
    15541 aattcattaa gacgatgaat tgaatcagaa tatttatttg tttcttgagc agcagcagac
    15601 aaaccagcaa atgcctctga caagggaaat actgttagaa caaatgctgc aatccagtta
    15661 gcttcgccac ctctgtgacc aataaactga ttactagccc aaataataac caaaatagct
    15721 agaaagccaa aaactaattc cacaataaga gctctacgat tatcaaagga acgaattttt
    15781 ttctgaacag ccatgagttc actttccgaa cgctcatgca aggcgacgta ctcttgacca
    15841 cgttgactaa aaatccagtc tgaaatacct aagacattat ccgtcaaatc agtataaaga
    15901 tgatttttta attctttttc acgagtctga cgagcaccat tagctaaaat cgaccaaagt
    15961 ggaaaaacaa aaatcataat agctaaataa agcaacatca ttaaagcgaa ccatagagaa
    16021 aagaagcctg ttgcaataat aataaagcta tataataacc accctataat cgttggaaat
    16081 attgtcctaa gatacaaatt ttgcaaataa ttaatatcct ccgctaaaag ccccataaca
    16141 tctcctaaac gaaaatcacg cttcataaag atagcattgc tttctaagga atggtaaagc
    16201 ttcaagcgca gttgtgaagt cattcgtaat acccaattgt gactcgttag gcgttctata
    16261 taacgaaaaa caggacgtcc aataccaaaa gcacgtgtta gtacaattgg aacatacact
    16321 agaagaatat tactcggtag tgaagctgac ttactaatca gatagcccga attaaacata
    16381 agagcactgg cagaaaagaa agtcataaaa ccaaggaaaa gagcaattac taaggaaact
    16441 ttatattgtt tgaaaaatgg ttttacccat tgatctgtct ttaattcttt aaacaaaggg
    16501 attttaaaca ttatttcctc ccatagcatg cttcagttga tagagatatc cttttttagc
    16561 taataattcc tgataagagc ctacttctgc aactctcccc ttctccataa cgactataac
    16621 atccatttgg ttcaaccagt gaagacgatg agttgcaaag ataactaaac gatctgacat
    16681 cagtggcaac attttctctt ttaattctaa ctctgtttca atatctaaat gagcagttgg
    16741 ttcatcaaaa accataatac gacgattttg atcaagaaaa gcacgagcca gagctatacg
    16801 ctgggcttgt cctccactaa gtggacgcgc tccattacca atacgcgttt ctaaaccatc
    16861 gggtaactcg cttagtaagc tatctaaacc taccatatga atcgctctta tcacctcttc
    16921 gtcactagca ttgggagtat agaaggtaat attatcacgt aatgacattt caaaaacata
    16981 aggagtttga ggaatataga tcatctgttt atgccaatct tcttgatcaa ggttaaccac
    17041 ttctctacca tccactttta aagaaccttt atcaggccct aaaaaaccac tcagaaggtt
    17101 aattaagcta gacttacctg aaccactaac tcctactaaa gcaacctttt gatgtcctct
    17161 aaatcgtaag gttacatcct caacaacacg tttttggtcg tacgcaatag caatgttttc
    17221 taaatccaaa tgactctctt tagaccaatc atctatgact aattgctcac ctttaatacc
    17281 agttttgttt agaacttttt gaatcgcttg aaatgcattt ttcccgtcta acgttgcatg
    17341 ataatcacta gaaaaatctc ttacgggtaa aaagtattca ggggataaaa tcaaaattgt
    17401 taaagccggt aaaaggtaaa tctgctcatt taataatcgt aaacctagaa aaacagcaac
    17461 gatagcaatg gataaagttg taaaaaagtc taaagcaaac gttgataaaa taccaatttt
    17521 caaagtactc attgttgctt tacgaaaaga ttcactagtt tgataaatgc ttttaccata
    17581 acgtttacta agaccaaaat atttgagagt atcaatgcct cgtaatgaat ctaaaaaatg
    17641 attagaaagt acttgatagg attcatattg tttatctgct ttagcttgag ccgcatagcc
    17701 taaaataatc ataaagatga taattaaagg aaaaacgata agtaaaattg cacctgattc
    17761 aatatctaaa tagaaaatat aagctagaat aatccatggg ataatagaca tgttaatcat
    17821 cttattcaag actaaacgta aataattttc aactaaagaa acgccatcta atgccattgt
    17881 aacaacatta ccagttcctt gttcttgaac gactttagga cctagatcaa ataacttatc
    17941 taataattga cgtctcaaaa gactagattg tgctgttgaa aagtcatcaa gttttctatt
    18001 tttgatgtaa tcaataacat gacggcctaa atatgagacc ataaataaca aaatataaac
    18061 cgtttgagaa gaaagttttt gcccttccca caaaccagtt atagaaagac tcaaataata
    18121 agcttgtccg ataataaaga ttgcttgaag aacatcaagt cccgcaagca atcctaacaa
    18181 cttatgaata cctgacaagc gcataaccgc tttatctagc attatttttc tcctgtcaca
    18241 atcactggca aggtaatacg ttttctaaag atataatatg cccatgctgt ataagctaga
    18301 acaaagggaa ctagagtaat tgcgacaatc gacattactt ttaaagtata tggcgttgag
    18361 gatgcatttt gaatcagcaa atcatattta gggctaattg atgaaatcat aactcgtggg
    18421 aatagacctt ggaacaacag aacaaccact gaaacaagac tgagaccact cgctaaaaat
    18481 gctgtcatct ctgctccttt aaaagttgaa gcatgcgcta atacagctaa tacaacaata
    18541 accaagacca taattgtcgt aactatcgga tgattgctaa agaaatctgt cttgaaatac
    18601 aagagaactg caaacaaggc taaaccaaga tagagtaccc aataaagcaa ctgagcataa
    18661 ttacgggcac gctctctaat cggcccttct gtcttcaatg caatgtagtt taaaccatga
    18721 agataggcta gcaataccat tgcaactcca ccaaccaacg agaatatatt aaagtaatca
    18781 ctgaattgag ctgataagtt accactggca tcaagtggca taccttgaat caaacttata
    18841 aacataatcc caaagaagaa tggaacaata gcggaaccaa ttgttaaggt ccagttccaa
    18901 aactgctttt tctctgcagg aactttatgt ctaaattcga aggaaacacc acgaataatt
    18961 aatccaaaga gaatcgtaag gagaatcaaa tagtaaccac taaatagtga agcataccag
    19021 tatggaaaag aagcaaacat tgctccacca ccggtaagaa gccaaacttc attaccatcc
    19081 caaactgggc caatggtttc tactacttga tctttttcat gctcattatg tgttaaagtt
    19141 tgaacagcca tcccaacccc aaagtcgaat ccttcaagga agaagaatcc tgaaaatagg
    19201 aggccgatta ggaaaaacca aaagaattgt aaagcactca tttagtatgc tcctttctca
    19261 aagggatcaa ttgaagtata tgcaccatga tgtccatgtt catcttcata ctctggccct
    19321 ttcttcaatt ctcttattgt taggcttatc atcatcaccg ctaaaccacc aaacaacaag
    19381 aagtacacag tattggaaac aaataatgat gcaactgaaa cgtttggaga gacagagtcc
    19441 ttaatcttga aaagaccata gacagtccaa gggtatcgtc cttgttctgt cacaatccaa
    19501 ccaaatgtat tggctaagaa tggtgcaaag gttgttaaag caacaatcca aagcatccat
    19561 ttgtgtttat aaagaattgg ttttttctta cgagttaaaa agaggcctaa aacagaaaca
    19621 cccataatag ctgttcccat cgccgccatt gtacggaaac catagaaaag taggttaacc
    19681 attgggtagt agttatcttt accatactta gcaaccaatt ctttattagc tgtatccata
    19741 cctttcacag aaccagatgg ttttccgtaa gataaaatac tcaacatata gggaatcttg
    19801 atcccaaaaa cttgtttgtg ttctgcttca tttgcccaag caactacact ccaagctgca
    19861 ggatcacccg aatcttcata atcaccttcc atagcagcaa atttcattgg ttgatcgtga
    19921 atcaaggctt tcatttgtaa atccccaact cccataactg aaattgaacc aagaagtgct
    19981 actactaaac caatacgaac tgatttctta taaagttcaa ccgttgtatc tttcagctgt
    20041 tctgatttta agaggcggaa ggctgacata ccagcaacca ctgtaccgcc catcgtaatg
    20101 gcaccaaaaa taacatgggt aaattcatag aagaactgtg gattttttac aagtgcaaga
    20161 aaatcagtca tttgcgctct accgtttaca acttcataac caacaggatg ttgcatgaag
    20221 ctattagcag taagaatcca catcgctgac attaatgagc caaaaaccac tagccaaatg
    20281 aatgtcacat gtaatttttt actaattttc ttgttatccc atgtaaacat ccataaacca
    20341 aggaaagtag attccataaa gaaagctaat aaagcttcaa tagccaatgg agctccaaaa
    20401 atatcaccta cgaaacgtga atagtctgac cagttcattc caaattggaa ctcttgaatg
    20461 atacctgtca caacaccaac tgcaaaacta aggagcatga tattacccca aaattttgtc
    20521 aattttttat actcttcatt ttttgtgata acatacattg tctccataat agcaaccact
    20581 aggcatgtac caattgtaaa tggtacaaag aaaaagtgga aaactgtagt catcgcaaat
    20641 tgaaaacgag ctaaagtttc aattgtcata caaaacctct tattttgttt taatcatttc
    20701 ttttatagaa atatctgcaa gagctagttt cctagcttcg taacaattgg taaataactt
    20761 actttaatat cttaatgata taaatcaaaa cgtcccttag ctagaagttc tttaagtcca
    20821 cccatatcaa caagtgattt gttcatgata atcttcttca taaccgatgc agggtatttt
    20881 ttaattttag tttttccaac aacaccaagt ccatgcgtat ttcctactga agcaactgtg
    20941 ccttgcggac tatacgaaaa gtcttctgtg gcttcacctt tgatttgatg taaaaggttt
    21001 ttagcaacat gagctcccat acgagttgca atttgagctg ttgttgggaa tgggcgacca
    21061 gactcagtgt ccataaatgc agacacatca ccgataacat aaacattatc atattttgga
    21121 tcacgcaaat cactgttgac cattacacgt ccacgacgtt gatcaaatcc tgattctccc
    21181 ataaccggac ttccactaac acctgttgtc caaatgatag tacctgcggt aatagatttg
    21241 agttcttcat cctctttact agtaccataa acaacttcac ccggtttgat ttccttaatc
    21301 atacttccaa gcatcaaatt aatacctaaa tccttaatta agttgacacc atattgcgct
    21361 aattcatcat caaacattgg taggatgcga gttgctgcct ctacacaaat aatttcaatt
    21421 tgttcaggtt ttacacccgc taaagcagca tattttggtc gctcatcaac catagctccg
    21481 gctaactcaa tacctgtaaa accagcacca catactaaga gtttgagaag attttcatct
    21541 tttgtctcac gatacaactt cattgtatta acaatgtgat tatggatgtt ttctgcttgc
    21601 gaaatattgg tcatctgcaa agcattctcc atagcaccct tgatgccaaa agtttcagat
    21661 acaaagccta aggcaactac gacataatca taatggagtt caccgttatt tttaactgtt
    21721 actgttttat tttcagcatc gactctaagc acctcatctt gcatgaagtt aaccttatta
    21781 gtgttgataa catccctaat tgggaatgta attttttctt ttggctgaga tcctgcagct
    21841 acttcatgta attctgtagc ttcgtagtga taatcattca tgtcaacaag ggtaatatga
    21901 aaatcacctg attgtttttg taaattacga actgctttca aaccggcata accagcacct
    21961 aaaactagga tttctttcat gataaaactc ctctaaaaaa tgattccaat tagatacgtt
    22021 aagacttggg ttgcagatcc cagtgctaga atacgtaccg cacaaacaaa tgtttctctc
    22081 ttaacctgtt tttgccacaa tagttttgtc tgcttataaa taaatggtat taataacaaa
    22141 ctcaagagta ctgtaacagg cgcaagtcca aaaagaaatt caaaaacaat tgccaataaa
    22201 gctatactat tagaaatagc aaacagcaag agtccacctc ttatccctgt ataatgtaca
    22261 agggtatagc gatgattttt ttcatcttct tctttgtcgc acagattatt cgccaacatt
    22321 aggttggcaa tccataaggt gttcggcaag gaaatcaaga aaattttgct taagttgagg
    22381 atatcccacg aaaagttttg ataagtgttt agatatacac aaattaaact aatcataaat
    22441 cccattgtca agccagaaaa tacttctcct aagggcagac tagataaagg tcttggacca
    22501 aaggaatata aaacaccaat tccaaaacaa aaaagtccca tccataaaag aggcagaccg
    22561 acttgggaaa ctaaaactaa accaatcata ctagaagtaa tgactaaact tgccataata
    22621 acttctattt ggcgtaggga taggttctca cgaccaataa tgttagtgtc attcttataa
    22681 tccaaatcca cagcattccg ataatcattg taattattcc aaatgtcaac gaacatattg
    22741 aataaaaaca tagcaacgaa gaataaaccg acatatacag ggtgtactga attgtagtaa
    22801 taagcactaa aacacaaacc aatcaaaaat ggtaaaacac tagctgtttt ggctttcatt
    22861 tctaccaact ctagaaagat tggtaatgtc attattaaag tcctctctat acataatctt
    22921 caacaatact attataatgc aatttctacc atttttaaaa caatagggct tgatttttga
    22981 ttaaaaatac aaaaaatttt tcttaaaatg agttaatcac acttagaaca ctaaatacta
    23041 ttttgaggag tcctattcat cacactatgc catgtaatat tcggaaaaat agtattgcaa
    23101 ccttcaatta ctccttatgt tggcaccagc ctttgaaagt cctctgattg cactgcttag
    23161 ttagtatttt catcagctgt atggtctcta agagccatca gcatactaaa aaaactagtg
    23221 ttacactttg aaaagtgtaa cactagctcc aatttgtttt aaatagctta actatttaat
    23281 cattaatcta ctttatctat ttaaacattt aaagttgaat gctggttaag gatacctgag
    23341 agagaacctt ggatatattt tggtaatact tgtttttggg cacgttcata gatttctgaa
    23401 tctgttaact gacgttctgc tttggggctt gatacttgcg gtgtcactgc tgcgttttgt
    23461 tgtttgtaaa gtgttgaatg ttggttaagg atacctgaga gagaaccttg gatatatttt
    23521 ggcaatactt gtttttgggc acgttcatag atttctgaat ctgttaactg acgttctgat
    23581 ttaggggttg atacttgcgg tgtcactgct gcgttttgtt gtttgtaaag tgttgaatgt
    23641 tggttaagga tacctgagag agaaccttga atatattttg gcaacacttg tttttgggca
    23701 cgttcataga tttctgaatc tgttaactga cgttctgctt tggggcttga tacttgcggt
    23761 gtcactgctg cgttttgttg ttggtaaagt gttgaatgtt ggttgagaat acctgaaaga
    23821 gaaccttgga tatattttgg taatacttgt ttttgggcac tttcataaag ttctgaatct
    23881 gttaactgac gttctgcttt ggggcttgat acttgcggtg tcgctgctgc gttttgttgt
    23941 ttgtaaagtg ttgaatgttg gttgagaata cctgaaagag aaccttggat atattttggc
    24001 aatacttgtt tttgggcacg ttcataaagt tctgcatctg ttaactgacg ttctgctttt
    24061 ggaggtgttg cttgcggtgc tgtgaaagtt gaagctgaaa caccttgtgt agaaccaaga
    24121 gctacgaatg ctcctccagc aaacattgct gagacaagac cgagtttcaa atagttagca
    24181 gttgagacgt tgttgacttt tttttctgta ataagttttt tcataagaat tacctctttc
    24241 ttttgggtta tttattttat gaactaagta taacaacaag aaataaagta aggttaaact
    24301 aaatataaaa taattcttaa gtcattctta ggaaaccata aaatcataca ctaacaaaaa
    24361 atgaggtgat aatactgtat gtattagata tttattctat tgtataaaca gcaatcaaaa
    24421 gtactttaaa cttcagggag caggatcatc acaattaaat atatcaaaaa aagtgtagaa
    24481 aattttacat ttctaatacc aaattttaac gaacaagaag ccattggtga cttcttccaa
    24541 accttagacc aacaaattac tcaaacggaa gaaaaactaa cagaacttaa agcactcaaa
    24601 caaacattac tcaatcgttt attcgtataa gaaaggacgc acatggcaga gaaaataact
    24661 tcactacggc aagcactatg gaattcggcc gatcaattac gtggtcaaat ggatgccaat
    24721 gactataaaa actatcttct tggactcatc ttttataaac acttatctga taaattactc
    24781 ttggctgttt gtgataattt agaagaacac tttaatacct ttacagatac tcaaaaaatc
    24841 tttgaagatg cctatcaaga tgaagaccta aaagatgacc tcatttctgt tgttactggt
    24901 gatttaggtt actttattga accgacactg acctttgaaa aactcatcca agatgtttat
    24961 cacaatactt tccaattaga atcattagct caaggttttc gcgatattga acaaagtggt
    25021 gaagattttg aaaacttatt taaagatatt gatctttact ctaaaaaact aggttctact
    25081 cctcaaaaac aaaaccaaac cattgccaat gtcatgaaaa cattaaatga gattgacttt
    25141 gagtcagttg atggggatac tttaggcgat gcttatgagt acttgattgg tgaatttgcc
    25201 agtgaatcag ggaaaaaagc aggtgagttc tataccccac aagctgtctc tcacttaatg
    25261 actcagattg tcttttcagg tcgtgaagat caaaaaggaa tgactctcta tgaccctgct
    25321 atggaatctg gaacactcct tctaaatgct aaaaaatata gtcatcaatc agataccgtt
    25381 tcttactatg gtcaagaaat caatacttct acttataacc tggctcgcat gaacatgatg
    25441 cttcatggcg ttgccatcga aaaccaacac ttaagtaatg ctgatacctt ggatgcggac
    25501 tggccaacag atgagccaac caattttgat ggggtgttga tgaacccacc ttattcttta
    25561 aaatggtcag cgactgctgg tttcttaacg gatccacgtt tttcaagtta cggtgtatta
    25621 gcaccaaaat caaaagctga ctttgccttt ttacttcatg gtttctatca tttaaaaaat
    25681 acaggaacta tggcaattgt cttaccacat ggagttcttt tccgtggagc tgcagaaggt
    25741 aaaattcgcc aaaaactact cgaacaaggg gctatcgata ctattatcgg acttccatca
    25801 aatatctttt ataatacaag catccctacg actattatca tattaaagaa gaacagaacc
    25861 aacaaagatg tcttctttat agatgcttca aaagagtttg ataagggcaa aaatcaaaac
    25921 acaatgacag atgctcatat caagaagatt cttgatgctt ataagagtcg tgataattct
    25981 gataagtttt catatttagc ttcatttgat gaaatcattg aaaatgacta taacttaaat
    26041 atcccacgct atgtcgatac ttttgaggaa gtccctgtta aaccacttcc agaattagcg
    26101 aaacaactca atgatattga tcaagaaatt gccaaaacaa atgccaaact agatcaactc
    26161 atgaaacgac tagtcggaac aactaaggaa gctcaagatg aattggatgc ttttagaaaa
    26221 taataatcag gcatgtgtca aacttcccaa cagtcaattt tgaagttgaa tcaaaaaaga
    26281 tataacccct tatttctcag atatgtttca aaaagtgaat aaaacaaaca cctttatata
    26341 tttttaaaaa gcaacctaga attgagttat taggtgaaaa tgatataatg aatgtcgtac
    26401 aaaaaaagat tgcatctgct tttgatcatt tttaagaata gggcagtgta aggcaattat
    26461 aaattaacaa caaggactaa tcccttgata tgaaaggaat ttattaatta ttatgatgaa
    26521 tatgcaagac ttgttccatg attttttatg tcttgtaatc atcagaaatg ttgattttaa
    26581 gccttttaaa
//
LOCUS       DATRSD010000018        26271 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRSD010000018 DATRSD010000000
VERSION     DATRSD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26271
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.018"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..283
                     /locus_tag="VBD85_000616"
     CDS             <1..283
                     /locus_tag="VBD85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7133185.1"
                     /translation="GQLIGKNPLLSKSTISRENNHSSQGDSNKQSFSKKVSQVTNVAN
                     RPMLTNNSRTISVINKLPKTGDDQNVIFKLVGFGLILLTSRCGLRRNEN"
     gene            490..1566
                     /locus_tag="VBD85_000617"
     CDS             490..1566
                     /locus_tag="VBD85_000617"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO7133186.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNARAMTGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIADF
                     PGAVLQDLPSQQIYPQAINAVGYRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            1736..2074
                     /locus_tag="VBD85_000618"
     CDS             1736..2074
                     /locus_tag="VBD85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133187.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2205..3746
                     /locus_tag="VBD85_000619"
     CDS             2205..3746
                     /locus_tag="VBD85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7133188.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQNFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTAGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            3786..5018
                     /locus_tag="VBD85_000620"
     CDS             3786..5018
                     /locus_tag="VBD85_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO7133189.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5231..5932
                     /gene="pyrF"
                     /locus_tag="VBD85_000621"
     CDS             5231..5932
                     /gene="pyrF"
                     /locus_tag="VBD85_000621"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO7133190.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRSSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQIQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            5945..6574
                     /locus_tag="VBD85_000622"
     CDS             5945..6574
                     /locus_tag="VBD85_000622"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO7133191.1"
                     /translation="MDLARQIAMELLDIQAVYLRPKQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQEHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREANIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            6586..7878
                     /locus_tag="VBD85_000623"
     CDS             6586..7878
                     /locus_tag="VBD85_000623"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO7133192.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSPE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAQERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8043..8966
                     /locus_tag="VBD85_000624"
     CDS             8043..8966
                     /locus_tag="VBD85_000624"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO7133193.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQYLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            8980..10056
                     /gene="carA"
                     /locus_tag="VBD85_000625"
     CDS             8980..10056
                     /gene="carA"
                     /locus_tag="VBD85_000625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO7133194.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVETSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTLDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10087..13269
                     /gene="carB"
                     /locus_tag="VBD85_000626"
     CDS             10087..13269
                     /gene="carB"
                     /locus_tag="VBD85_000626"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO7133195.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRASRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESRAFTLKVLD"
     gene            13396..13719
                     /locus_tag="VBD85_000627"
     CDS             13396..13719
                     /locus_tag="VBD85_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133196.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            13716..14459
                     /locus_tag="VBD85_000628"
                     /pseudo
     CDS             13716..14459
                     /locus_tag="VBD85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611752.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
     gene            14451..14678
                     /locus_tag="VBD85_000629"
                     /pseudo
     CDS             14451..14678
                     /locus_tag="VBD85_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
     gene            14760..17777
                     /gene="esaA"
                     /locus_tag="VBD85_000630"
     CDS             14760..17777
                     /gene="esaA"
                     /locus_tag="VBD85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HEO7133197.1"
                     /translation="MKRNNFRILWYIIAVALFLVAIAGLNLKLQGDHAKENKTTQSAT
                     NTKLNIALVNEDQNVSNGKESYNLGASYIKSIERDNSQNWSVVSRGTAQNGLNKGDYQ
                     LMVIIPNNFSQKLLDVNKANAEQTTISYKVNAKGNLALEKKATEKGKDIVSELNSHLV
                     NMYMASILSNLYTAQENVQAMVNVQSGNISNYQKNLLDSATNFQNIFPALVNQSSSSI
                     TANESLKKSLEASDNMFNDLVTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNE
                     LLEKQLSDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSE
                     ALKSQDNVAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSNNEKTLKDFKAK
                     VADFTNSLISSIPSLNLSELHLTQEEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNR
                     LFEARKNRDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDP
                     SQNNNIIINSSYQGESSNSSITKPSYTITIGNQKQIVQNDDGKTQRSYLETEATYQRT
                     LQEVIDAYNTTHNLVSKYYIFSDGEEPQSLFDQFLNQSVNDTMVDLVKNGITKYLMDE
                     NTADAQQKVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQSINMKVQNI
                     TQDQSKVNDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNK
                     DVQDAKNSGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFL
                     SRPVAESASAVRATENTYRPFTWILLLEVVSLFTAYIFATQNLIKKLTDRYNVNRWLQ
                     TDFLNVIVISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIK
                     NFKAVGMGLALFMIISFVYLSNAVGTVATVSGLPKLLKAINPLSILENQLSSYFDNVT
                     TGFIFLILVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            17786..18259
                     /gene="essA"
                     /locus_tag="VBD85_000631"
     CDS             17786..18259
                     /gene="essA"
                     /locus_tag="VBD85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7133198.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDENRQSKVSLQKKQSKQLFQYSNKGQSDRKKRIKQLF
                     SEESNQFKFVTRTEEPTVSADIGHMIMYLVLISLVLILASIMTFKIKDKKDEYTY"
     gene            18243..18485
                     /locus_tag="VBD85_000632"
     CDS             18243..18485
                     /locus_tag="VBD85_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO7133199.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            18485..19762
                     /gene="essB"
                     /locus_tag="VBD85_000633"
     CDS             18485..19762
                     /gene="essB"
                     /locus_tag="VBD85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO7133200.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAIDADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKELSSEPSSTTTSTSSKTKSR"
     gene            19769..24178
                     /gene="essC"
                     /locus_tag="VBD85_000634"
     CDS             19769..24178
                     /gene="essC"
                     /locus_tag="VBD85_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO7133201.1"
                     /translation="MLVICYYQSLRYEFNIEEEKSFLISSNGKSPIPVSELENDITLK
                     NMQGQLVYIIDQKEKELTNGVEISGIVFYLANNQKEIYTPLDYEDILIGDKEGYHIRF
                     KEGAPNLLLKKIESNWQLNLFEGDIYLNNHLQKVVQQLPLSLGDEISFQGIIIKLFPD
                     EIQIWGGTDYETSLTKKVMSAYQFYAGYPDFHRSPRIIYRSSEDKITVNAPGNEPNKS
                     KDELLKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMIFSITGYFKNRKQYKQ
                     DLQERIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQEMADTYHHRIYEKTPL
                     HFDFLYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYFNNRYIKNMPIVANLS
                     HGPVGYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEEMDKWSWMRWLPHATL
                     QDVNVRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTLFSPHYVVIVTDEKLI
                     LDHVIMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDRNTGQLVIEEGELKET
                     DFELDHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMYQAEEFEDLHVQERWI
                     SHAPYKSLAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSGKSEIIQSYILSLAVN
                     FHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRLF
                     AKADVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPEFMKELVSTARIGRSL
                     GIHLILATQKPSGVVDDQIWSNSRFKLALKVADRGDSIEMLHTPDAAEITQAGRAYLQ
                     VGNNEVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYEILNDDLSGLDQAENI
                     KEVPTELDAIVENIQALTKEMGISDLPQPWLPPLSNQIAVTDLRKEESVDLWSKAPSY
                     KAVLGFMDIPSQQAQEVAWHDFEDDGHLSIFAGPSMGKSTALQTVTMDLARHNSPEFL
                     NLYLFDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFIARIKVEMSDRKKALSRYNVAT
                     AKLYRQVSGETMPQILIVIDSYEGLREAQTLTNLEACFQNISRDGSSLGISLVISAGR
                     MAALRSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGRGLIKRDDIEVLQVAL
                     PTEGTETFDIISNIQTESDAMNDKWTGPRPKAIPIVPEELTFDDFMATDSVQADLSAN
                     RLPLGLEMVDVESFSLPLTKFKHLLFLSDSDEGLENLGSHLLKTLIKVPEYSTMIIDS
                     LGEHEAYQGQVRTYVGADMISDMAEQLNYELGKRQEQNAFDRWFILIPDFESFVSKTN
                     LSLEQIQNLLDNGPKVGLHLIIGSAFSFVGSKLDPVNKYVKTNSQYVMLGMRLMDQTF
                     LEKVYNSKEARLERDEAYIHDRKNYQKLKLSID"
     gene            24191..24526
                     /locus_tag="VBD85_000635"
     CDS             24191..24526
                     /locus_tag="VBD85_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HEO7133202.1"
                     /translation="MAKIQSDASLASSCATSIQTGANGITSVGKATKDSDSQYSGQTS
                     ASLYIDNEASKSGSIAGKLTEFIGLIHSTASEFEAVDQKLSQSISTPKQYTTPSGNST
                     TFQPKPYFN"
     gene            24535..24891
                     /locus_tag="VBD85_000636"
     CDS             24535..24891
                     /locus_tag="VBD85_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133203.1"
                     /translation="MNDKQLKEVERNEAILRKELERIEDKKIVLKKSYDKTINMQLDI
                     QQSLRDSSQSLSPEEVMEQEEIMLIFNRQSRIVEDYFQEEMAKLNKQETDAKDTLEGL
                     VQERQKLYVSQSEKGE"
     gene            24891..26177
                     /locus_tag="VBD85_000637"
     CDS             24891..26177
                     /locus_tag="VBD85_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133204.1"
                     /translation="MVKVNAGSLAAQASSVSTVVASRVTALNSAKQSLTSFANQAGLE
                     GTAYQNAKSYATGVLGTLIDGMILLSEETEEGVSKLQSLYSDKCGGESLDSDVLTKKI
                     EKDEELLAKIHDLQAHLDKHATFLSHAFDGLEATIKARLKKNKKKLKYLMEFNAESST
                     VFDGLTEVQDAVSAGLNYVSEGFGNFNGTFDTSGKGLDWTKTIKTGMEKRRDKVSIDK
                     NSLLKEVKKAYKAGKINKQTYQSIKSGLVNSSTAFLKELAKAKLTDKAVETFTKESWE
                     ALSKNFQTMQFSGVLAAVNGAELSTAIDVNPAYAEMIRGGSQFVKKGVPVIGAAVDFG
                     LQLKDGEDVGDAAVKTGGHVAIGIGAAKAGAIIGSAFPVVGTAGGAVVGFAIGVAGSM
                     AFDWLYDNKEQVYNTTKKVISTRKTIDSVVYEGFWR"
BASE COUNT         8948 a         4332 c         5018 g         7973 t
ORIGIN      
        1 aggtcaactt atagggaaaa atccacttct ttcaaagtca actatatcta gagaaaataa
       61 tcactctagt caaggtgact ctaacaaaca gtcattctct aaaaaagtat ctcaggttac
      121 taatgtagct aatagaccaa tgttaactaa taattctaga acaatttcag tgataaataa
      181 attacctaaa acaggtgatg atcaaaatgt catttttaaa cttgtaggtt ttggtttaat
      241 tttgttaaca agtcgctgcg gtttgagacg caatgaaaat taagtataat caatcattta
      301 gtaactatta taatgatata tgcaatcaat aaaaaggaat cggatacaag attccttttt
      361 ataattaggt tggttagggt gactttttca tttggctatt cttgaaagtt tataaaaatg
      421 tagtataata gtcacattaa aatgttttga aaatattgat gaacaacatc aacaaataga
      481 ggtcattata tgggatatac cgttgctatc gtaggtgcta caggtgccgt aggaacacaa
      541 atgattcgtc aattagaaca atcgaattta ccaatagaac aagtgaaact tttatcatca
      601 agtcgctcag caggtaaaat tttacatttt aaagatgagg ctatacgtgt tgaagagaca
      661 acaaaagaat cattttacga tgttgatatt gccttgtttt cagctggtgg atctatttca
      721 gcaaagttcg ctccttatgc agtaaagtct ggagcagttg tagtagataa cacgtcatat
      781 tttcgtcaga atcctgatgt tccactagtt gttcctgaag taaatgctcg tgccatgact
      841 ggtcataatg gtatcatagc ttgccctaat tgttctacta ttcaaatgat gattgcttta
      901 gagcccattc gtcaaaaatg ggggatagag cgtgttatag tttccaccta tcaagctgtt
      961 tcgggttcag gtgcacgtgc tgttgaagaa actaaggaac agttgagaca agttttaaat
     1021 gataatcttt cacctgatca attaattgca acagtcttgc catgttctag cgatcaaaaa
     1081 cattatccta tcgcattcaa tgcattacct caaattgata tttttactga taatgattat
     1141 acgtacgaag aaatgaagat gactttggag acaaagaaaa ttatggaaga tgctactatt
     1201 aaggtttcag ctacctgcgt ccgtattccc gttttatcgg gtcattctga gtccatttat
     1261 attgagacga aagaattagc ttcaatctct gaaattaaaa aggctattgc agattttcct
     1321 ggtgccgtcc tacaagacct tccatctcag caaatttacc ctcaagcgat taatgctgta
     1381 ggctataggg agacctttgt tggtcgtatt cgaaaagacc ttgatcaaga aaatggtgtt
     1441 catatgtggg tagtttcaga taatctattg aagggggcag cttggaattc tgttcaaatc
     1501 gcagaaacat tgcataagaa tgggctggtt aaaccagcga aagagttaaa atttgaatta
     1561 atatagaaga atatgacatt gcttaatact tatgctgact gctagtgata ttgattttta
     1621 atcaatatca ggtaaaacac ttgacaaatt atgaaaacat ttgcataatg aaattaacaa
     1681 atgaaaatga ttgcaaatca ttttaacatt gtttccacat taaggagggg ctcttatgca
     1741 aatcatcaaa cgtgatggac agattgttga atttgatcct gaaaaaatct accaagctat
     1801 cttgaaagct gctcaaacag tttatgtgct tgatgagtca cttcgtcaag accttgctca
     1861 agtgactaaa aaagtaatct tagatttaga ggatgctcag gttgaacgac caacgatcaa
     1921 tatgattcag tctttggttg aaaatcgttt attggatgca ggatacattc atattgctga
     1981 acactatatt tcataccgtt tgcaacgtga tttagagcga aatggttatg atgatcaaat
     2041 cactgttcat ttacactttg aaaaaattaa gtaaggtttg acttttaagt ttaaactctc
     2101 tatacttgta tatggaactt actatgtaat gtactaattt aaaaacttgt agagtgtctc
     2161 tatgagtttt ttaataacct ctattatcaa tcaaaggatt ttaaatgagt attttagaag
     2221 taaaaaactt aagtcacggt tttggtgatc gtgctatttt tgagaatgtt tcttttcgcc
     2281 ttttaaaagg tgaacatata ggacttattg gtgctaatgg tgaaggaaaa tcaacattca
     2341 tgtcaattgt cacaggtcat cttcaacctg atgaaggaaa aattgaatgg tcaaaatatg
     2401 taactgctgg ttaccttgat caacatactg tattagaaga aggtcaaaca gttcgtgatg
     2461 ttttgcgaac agcatttgat gaacttttta aaacagaggc acgtatcaat gatatatata
     2521 tatccatggc agatgatggc gcagatgtag atagccttat ggaagaagtt ggagaactac
     2581 aagatcgttt agaatcacgt gatttttata cacttgatgc taaaattgat gaagtcgcac
     2641 gggcattagg agttatggat tttggtatgg attctgatgt tactgaatta tctggtggtc
     2701 aaaggacaaa ggttctttta gcaaaactgt tattagaaaa gccagacatc ctcctgcttg
     2761 atgagcctac aaactacctt gatgctgaac acattgattg gttaaaacgc tatttacaaa
     2821 attatgaaaa tgcttttgtt ttaatctcac acgatattcc atttttgaac gatgttatca
     2881 atattgtcta tcatgttgaa aatcaagatt tagtaagata ttccggtgat tatactaatt
     2941 ttgaatcagt ctatgcaatg aaaaaggctc aattagaagc agcctatgaa cgtcaacaaa
     3001 aggaaatagc tgatttacaa aattttgtta atcgtaataa agcacgcgtt gcaacacgta
     3061 atatggcaat gtctcgtcaa aagaaattag acaaaatgga tattattgag ttacaagcag
     3121 agaaaccaaa gccaagtttt gaatttaaag aatctcgcac agcaggacgt tttattttcc
     3181 aagctaaaga tcttcaaatt ggatatgatc gtgctttaac aaaacctcta aacctaacat
     3241 ttgaacgtaa tcaaaaaata gccattgttg gagcaaatgg gataggaaaa acaacattac
     3301 ttaagagttt actaggtatt atccccccta tttctggtaa tgttgaacga ggtgatttta
     3361 ttgatttagg ttattttgaa caagaagtac ccggaggaaa tcgccaaact ccacttgaag
     3421 cagtatggga tgcttttcct gctttgaacc aagcagaggt tagggcagct cttgctcgtt
     3481 gtggtttaac ttcaaagcat attgaaagtc agattcaggt gttatctggt ggggagcaat
     3541 caaaggtcag attttgctta ttaatgaatc gcgaaaataa tgtccttgta cttgatgaac
     3601 caaccaatca tttagatgta gatgctaaag atgagttaaa acgtgcttta aaagcttata
     3661 agggatcaat cttgatggta tgtcatgaac cggactttta tgaaggatgg atggatgatg
     3721 tttgggactt taaccaactt agttaagtgg ctagggtaat aaagtagaag gcagggccaa
     3781 ataagatgca aaatgatatg agcgttgttc ttagagcagg taaattactg attgaaagcg
     3841 gagcagaagt ctatcgtgtc gaagacacga tgaagcattt tgctaaggct cttcagatag
     3901 aaaattttga agcttatgtg gttagtagca gtattatcgc ttccgggatt aaccgctatg
     3961 gtaaacaaga agctaaagta tgcaatacag atggtgtaac agcaaattta ggtagacttg
     4021 aagctgttaa taacctttct aggcaaattg ctaaacaaga cttagtatct ccagaagaaa
     4081 ttgtaaagca gttagactta attgaacatc aaaaagatta tagtttactt gttactttaa
     4141 tttcttattt ttgtggagcg ggtagttttt ctttagcctt gggtagttcc ttattagatt
     4201 cattttccgc tgctgtaaca ggcttaattt taggatattt tctgaatttg atggaaagtc
     4261 gtatacatac aggatttctt ttgaccattc taggtagttc tgtagtggct ttaagtgcta
     4321 atttattata tttttcagga ttaggtgagc atcgtagcat catcatatta ggggcactaa
     4381 tggttatggt gccaggggct gcctttgtta attcggtgag agaattttcg cagaataatt
     4441 tttcaacagg tttagctttg attatgtctg ctttactcat ttgtatttct atatcagcag
     4501 gcgtggctat tacaattgaa attattccgt ttgcagaaca aatgacaggt tctttttctg
     4561 gtgttcctaa taccatcctt gagattatca tcagaacact aatggccggt ttagggacga
     4621 tagccttctc tattctctac catgttccaa aacgttattt ccttgactta ggtatactag
     4681 gagcaatatc ttggatgctc tacctgatct tatggcaaca atttcatatg gatgctatag
     4741 ccgtattttt tccagggctc ttcatcactt atttttcacg gctattagct gctaaacgta
     4801 agtgtcctgc cactgtcttt ttagcgacaa gtatgtttcc tcttattcca ggacttagct
     4861 tctatagagc tgtttacttt ttattgacgg gagcagatgc cttagcaatg gaatatttta
     4921 gatcctgttt tgtaacagct tttacaattg ctatagcaat cagtattgta cagcagattc
     4981 ctctatcgtt ctttattaga cgtaaaatga taaaataatt tagtatttat ttgacaaaat
     5041 gattgtaaac ttgtagaata cctacaaatg tatctttaag tgagtccaga gaggcgaaca
     5101 aggtcaccat cataagtcaa atagcgagtg ctttttcgct aactttttat gaaaccttgc
     5161 cctcaggcaa ggttttttgt gtcatactgg tacatttcca acaattatta tttctataga
     5221 gaggaattat atgttagaaa aatgccctat cattgcctta gatttttctg atttggcttc
     5281 tgtaacaact tttttagaac attttccaaa agaagaattg ctttttgtca aaataggaat
     5341 ggagctttat tattccgaag gtccctcaat tattagatat attaaatcct taggacatcg
     5401 catttttttg gatttaaaat tacatgatat ccctaataca gttcgatcat ctatgtctgt
     5461 cttagccaaa ttaggcattg atatgactaa tgtccatgct gcaggaggag tcgaaatgat
     5521 gaaagcagct agagaaggtt tgggggaggg tccaatacta ttagcagtga cacaattaac
     5581 ctcgacttca caagaacaaa ttcaggtgga tcaacatatt aacttaagtg tagttgattc
     5641 ggtttgccat tatgctcaaa aagctcaaga agcaggctta gatggagtag tcgcatcggc
     5701 tcaagaggta aagcaaatca agaaacagac taacgaacat ttcatttgct taacgcctgg
     5761 tatcagacct ccacaaacca atcagttaga tgatcaaaaa agaacgatga ctcctgagca
     5821 agcacgtata gttggggctg attatatcgt tgtgggaaga cctataacga aagcagaaaa
     5881 tccatatcaa gcatatctag acataaaaga agaatggaat cgtattaaat aaaggagtac
     5941 tgagatggat ttagcaagac agattgccat ggaactatta gatattcaag ctgtttactt
     6001 aagacccaaa caacccttta cttgggcttc gggggtcaaa tcgccaattt acactgataa
     6061 tcgtgtaacc ttatcgtatc ctgagacaag gacactgatt gaaaatggtt ttgttaaaca
     6121 aatacaggaa catttcccaa acgttgatat catcgcaggg acagcgactg caggcatacc
     6181 acatggcgct attatagctg ataagatgaa cttgcccttt gcctatatac gctcgaaggc
     6241 aaaggatcat ggtgtgggga atcaaatcga gggacgtgtt tactcaggcc aaaaaatggt
     6301 tatcattgaa gatttgattt caacaggagg atctgtttta gaggctgtta cggctgcgca
     6361 gagtcaaggt attgaggttt taggggtagt agcgatattt acatatcaat tagctaaggc
     6421 tgaacaagca tttcgtgaag ctaatattcc tttggtgact ttgacggatt acaatcaatt
     6481 aattaaagtt gctaaagtta atggatatat tacagctgac caactggtat tactgaaaaa
     6541 atttaaagaa gatcaaatga attggcaaag ttagagagga cagatatgta tatcattaaa
     6601 aacggattga ttatcgaccc tcaaagtggt tttaatcaag tatcagacat gctgattgat
     6661 caaggaaaaa ttaaacaaat atcgaaagaa attgatatta aaggcattcc aatcattgat
     6721 gcaagcaata aaattgtagc tccaggatta gtcgatattc atgtacattt tcgtgagcct
     6781 ggacagacac ataaagaaaa tatccataca ggagccttat cagctgcagc aggtggtttt
     6841 acgacagttc ttatgatggc caatactaac ccaacgattt ctagcccaga aattgtaaaa
     6901 caagttaagg agagtgcagc aaaagaagca attaagattg aaacagttgc tacaattact
     6961 aaatccctga atggtaaaga tttagttaat ttcgaagaat tattagaagc tggagttgct
     7021 ggattctcag acgatggtat ccctttgaca gatactaagg ttttgcaaga agctatgaac
     7081 ttagctagaa aacatgatgt tgtcctatcg ttacatgaag aggatccatc gttaaatggt
     7141 gttcttggta tcaacgaaca tattgctcag aaaatttatc atgtctgtgg agctagtggt
     7201 cttgctgaat attcaatgat tgcgcgagat gctatgattg cttaccaaac acaggcgaaa
     7261 gtccatatcc agcatttgtc aagttcagaa tcagtagaag tagttgattt tgcgcagaag
     7321 cttggtgcta acttaacagc agaagtaacc ccccaacatt tttcaaagac agaggactta
     7381 cttttaacta agggtgctaa tgcaaaatta aatccaccac tgcgacttga aaaggataga
     7441 caagccctca ttgatggtct taaaagtggt gttatttcaa ttattgcaag tgatcacgct
     7501 ccccatcata ttatggagaa agcagcagat aatattagtc aagcgccctc tggtatgact
     7561 ggcttagaga catcattagc cttagggatt acttatttag tatctactaa agaactctca
     7621 atgattgact ttttggcaaa aatgacatgt aatcctgcac agttatatgg atttgatgca
     7681 ggttatctca gagaaggtgg acctgcagat atcgttattt ttgatcaagc tcaagagaga
     7741 ataataaagg cagaatttgc ctccaaatcc agcaactctc cttttatcgg tgataaattg
     7801 aagggagttg ttcactacac aatttgcaat ggtgaaatag tctatcaaaa agatagccac
     7861 gcagcaacat tggtataggt aaaacatttc ttattaatat cctttaaggt attgtccaga
     7921 gaggcaaaca aggaattgcg ttaacaaata gtaagttagt tccctatcta gcttactatt
     7981 ttgcagagcc tccttgtttt aggaggcttt tttctagcta aaatttatag aaagaaatta
     8041 tcatgaatca tcaaacacaa acactttcat tagaacattt tgtcagttta gaagaattat
     8101 caaatcaaga agttatgtct ttgataaaga gaagtatcga agttaaagaa aatcctagca
     8161 acattggttt tgataaagat tactatgtgt caaatctctt ttttgagaat tcaacaagaa
     8221 cacataagtc atttgaaatg gcagaactca aattaggatt aaaaacaatt gaatttaatg
     8281 ctgatacaag ttctgttaat aaaggagaaa ccctttatga cactatctta acaatgagtg
     8341 ctctaggatt ggacgtttgt gtcattaggc accctgatat tgactattat aaagaattga
     8401 ttgctagtcc gaacattcat tcagctattg tcaatggggg tgatggctca ggccaacatc
     8461 caagccaatc acttttggat ttggtaacta tttacgaaga atttggatac ttcaaaggac
     8521 taaaaattgc gattgtagga gatctaactc actcacgggt agctaaatct aacatgcaag
     8581 tgctaaaacg attaggagct gaaattttct tttctggacc caaagaatgg tatagtagtc
     8641 agttcgatga atatgggcaa tatttaccaa tagatcagtt agttgatcaa attgatgtcc
     8701 ttatgctttt acgtgttcaa cacgaacgtc atgatggtaa aggggtgttt tcaaaagaaa
     8761 gctatcatca acaatttggt cttacaaaag aacgctataa acatttaagg gatacagcaa
     8821 ttattatgca cccagcaccc gtcaataggg atgtagagat tgctagcgat ttagtagaag
     8881 cagataaggc tcgaatagtg aaacagatga gtaatggcgt ctatgcaagg atagctatct
     8941 tagaagcagt gctcaatagc agataataaa ggagataaaa tgaagcgtct attattattg
     9001 gaagatggta gtgtgtttga gggtgaggct ttcggagcgg atgtagaaac aagtggtgaa
     9061 attgttttta gtacaggaat gacagggtat caagaatcta taacagatca atcttacaac
     9121 ggtcaaatca ttacttttac ttatcctctt attggaaatt atgggataaa tcgagacgat
     9181 tacgaatcta ttagaccaac atgtaaggga gtaattgttt atgaatgggc agagtatcca
     9241 agtaactggc gtcaacagat gacacttgat gaatttttaa aattaaaagg aatacctgga
     9301 atttcaggta tagatactcg agcattaaca aaaattattc gaaaacatgg aacaatgaaa
     9361 gcttgtttaa taaacgaggg aaattctatt catgaagcac tagaaaacct tcaaaaaagt
     9421 gttcttttaa ataatcaaat tgaacaagta tcaaccaaat tagcttatgc ttcgcctgga
     9481 gttggcaaaa atattgtatt agttgacttt ggtttaaaac attctattct aagagaactg
     9541 tcacaacgtc aatgtcatat tacagtcgtc cctcatacta cgacagcgca agaaatctta
     9601 aatctcaatc cagatggagt actcttatct aacggccctg ggaacccaga acaattaccg
     9661 aatgctttac aaatgatcca agaaattcaa ggtaaaattc caatttttgg tatttgtatg
     9721 ggacatcaac tatttgctaa agccaatggt gcaaagactt ataaaatgac ttttggtcat
     9781 cgaggtttta accatgctgt tcgtcatttg caaacaggac aggttgattt tacaagtcaa
     9841 aatcatggtt atgctgtctc gagagaagat ttccctgagg ctctcttcat tacacatgaa
     9901 gagattaatg ataaaaccgt cgaaggtgtt cgacataaat actatcctgc cttttcagtg
     9961 cagtttcatc cagatgcagc accagggccg catgatacat cttatctttt tgatgaattt
    10021 attaatatga ttgatgattt ccagcaaaaa agctagcttt aaaatataga attagaggag
    10081 tttgatatgc caaagcgcac agacatacgt aaaattatgg ttatcggttc tggcccgatt
    10141 gtgattggac aagcagcaga gtttgactat tctggtacac aagcctgtct ctctttaaaa
    10201 gaggagggat accaagttgt tttggtcaat tcgaatccgg ctactatcat gacagataaa
    10261 gatattgctg ataaagttta tattgaacct attaccttgg agtttgtcac aagaattctc
    10321 aggaaggaaa gacctgatgc tctccttcca actcttggag gtcaaactgg attgaatatg
    10381 gctatggctc tatcaaaaaa tggtatccta gaagaattga atgttgaact attagggacc
    10441 aaattatcag ccattgataa agctgaagat cgtgatttat ttaaacagtt gatggaagaa
    10501 ctcaatcaac ctattccaga atccgagatt gttaattcgg tagaagaagc tattcaattt
    10561 gcagagcaaa tcgggtatcc attaattgtt cgtcctgcct ttactttagg aggtacaggt
    10621 ggaggcatgt gcgataatca agaacaattg gttgacatca cgacgaaggg gttaaagtta
    10681 tcgcctgtga cacagtgcct tattgaacgt tcaattgctg gctttaaaga gattgaatac
    10741 gaagttatgc gtgatgcagc agataacgct cttgttgttt gtaatatgga gaattttgat
    10801 cctgttggta tccatacagg ggactctata gtttttgcac ccgcacaaac gttatctgat
    10861 gtggaaaatc agttattacg agatgcgagt ttagatatta taagagcttt gaaaattgaa
    10921 ggaggatgca atgttcaact agcccttgat ccgaatagtt ttaaatacta tgtcattgaa
    10981 gttaatccaa gagtatcaag atcttctgct ttagcttcaa aagctactgg atatccaatt
    11041 gctaaattag cagctaagat agctgttggt ttgacactag atgaggtaat taatcctatt
    11101 acaaaaacga cttacgctat gtttgaacct gctttagatt acgtagtcgc aaaaatgccg
    11161 agatttcctt ttgataaatt tgaaagtggc gatagaaaat tgggaacaca gatgaaagct
    11221 acaggcgaag tcatggcaat aggacggaat atagaagaat ctttgttgaa agcttgtcgt
    11281 tcactagaaa taggtgtcga tcacattaaa atagcagatt tagataatgt atcagatgat
    11341 gttttattag aaaaaataag aaaggcagaa gatgatcgct tattttatct agcagaagct
    11401 ttacgtagac attatagtat tgaaaaatta gcaagtttga ctagcattga ttctttcttc
    11461 cttgataagt taagagtgat tgtagagtta gaagatctat tatctaaaaa cagactggat
    11521 atcaatattc tcaaaaaggt taaaaataaa ggtttttcag ataaagctat agcgagttta
    11581 tggcaaataa acgaagatca agttcgtaac atgcgaaaag aagcaggaat tcttccagtt
    11641 tataagatgg ttgatacatg tgcggctgag tttgattcag caacgcccta cttttattcg
    11701 acttatgccg tagaaaacga gtcattgata tcagataaag cctctatttt ggttttagga
    11761 tcgggcccaa tccgaattgg acagggagtt gaatttgatt atgcaacggt tcattctgtt
    11821 aaagccatca gagagtctgg ttttgaagcg attatcatga actctaatcc agagacggtc
    11881 tcaacggatt tctctatttc agataagctt tactttgaac ctttgacttt tgaagatgtt
    11941 atgaatgtta ttgacttaga aaaacctgaa ggggtcatct tacaattcgg tggtcagact
    12001 gctattaatc tagcaaaaga tttaaacaag gcaggggtta aaatactagg cacacaatta
    12061 gaagatttgg atcgtgctga aaatagaaaa caatttgaag ccactttaca agctctaaat
    12121 atccctcaac caccaggttt taccgccact acggaggaag aggcagttaa tgcagcgcaa
    12181 aagattggtt atcccgtgtt ggttagacca tcatatgttc ttggtggacg agctatgaag
    12241 atagttgaaa acgaagaaga tttacgtcat tatatgacaa ccgcggtaaa ggcaagccca
    12301 gatcatccag tgcttataga tgcatacctt ataggtaaag agtgtgaggt tgatgctata
    12361 tccgatggtc agaatatact gattcctgga ataatggaac atatagagcg agctggtgtt
    12421 cattcaggag attccatggc tgtataccct cctcaaacat tgtctgagac cattatcgaa
    12481 actattgtag attatacaaa acgcttagca attggattaa actgtatcgg tatgatgaat
    12541 attcagtttg taatcaagga tcagaaagta tacgttattg aagttaatcc tcgtgctagt
    12601 agaacacttc cattcttatc aaaagtaact cacattccta tggcccaagt agcaacaaaa
    12661 gttatcttag gagataaact ctgcaatttt acatatggtt atgatctcta tccagcttca
    12721 gatatggtcc acattaaagc ccctgtcttt agttttacca aacttgctaa agtagatagt
    12781 ttattaggtc ctgaaatgaa atcaacaggc gaagtgatgg gatcagatat taatcttcag
    12841 aaagccttgt ataaagcctt tgaagcagca tatctccata tgccagacta tggtaacatt
    12901 gtttttacag tagatgatac agacaaagaa gaagctttag aacttgcaaa agtctatcaa
    12961 agtattggtt atcgtattta tgctacccaa gggactgcaa tttattttga tgctaatggt
    13021 ttagagacag ttttagtagg taagttagga gagaatgatc gaaatcatat tcctgattta
    13081 attaaaaatg gtaagattca agcagttatc aacacagttg ggcaaaataa cattgataac
    13141 catgatgctc tcatcatcag acgttctgca attgaacaag gagttcctct atttacatct
    13201 ttagatactg cacatgcaat gttcaaagtt cttgaaagca gagcatttac attaaaagta
    13261 ctagattaat caaacggtca aggtaaaatt aacttccagc aaaacagaat cggactagat
    13321 taaatatagt ttgttctgtt tttataaaaa aagcctaatt gtgataaaat agggaacaaa
    13381 cttaaggagt taattatgtt aaaaacacga acacatatta atgaattaga aacaaaaatc
    13441 gcccttgatt tagtacacag atatctaggt attcaacaac ttgttttttc aaagtggagg
    13501 cattctttag aaactggttc atggtcttct attataacaa taaatggcgc taatattatc
    13561 aattacattg ttgatgatat tagcgacagt gatagtattt caggtattat ttgtcatcct
    13621 aaaagttttc atctcagacc tagagaaaaa aaggaaccta ttcagtctat tgatgagtta
    13681 gatatcatct ggtctttgaa tagagaggaa aatgaatgat tccagatagt agttttaaag
    13741 tgctatcaga catggcatat aatgttgaag aatccaaagc aaaaagtaat ggacacctta
    13801 aaattataga aggtagtcaa attattgatt tccatcaaaa acgctctttt atggtattag
    13861 aggttaatga taatatcgat aatggtatgc aggctatggc atgtcagtct ttggatgaga
    13921 gtaatcatat tgttatagca tattcaggaa cgaacgcttt taataagaaa gatatcttaa
    13981 cagatatcga ttcagttgta ttggggaaga acaataaatt gctaacttgt ccttcaagta
    14041 ttaccctgag agaggatggc atttctctgc cttgtaaaaa tccaagtcaa attacaacat
    14101 ctaaactatt tgcacaaaaa attaagatga actatcctga tgcaaccctc tggttaacag
    14161 ggcattctct aggtggttat ttggccatga ttgtggcttc agaaatgaga ttatcagcgc
    14221 gtgtttttaa ttcccctagt cccattaaca atatgtctga agcagccgcg tcctatgtaa
    14281 tttcacatcc agagaagtat cgtagttacc gtattaataa agattacatt ggtaattttg
    14341 gtattaatag ttcagatgat aaattaggta tttctaaaag agtcaatgct aagaagcaac
    14401 atggttttta taaaaagatt ttctttcacg aattgacacg atttcgatta aatcaacaag
    14461 taacagaagt tcttaatttg cttactcaag agcaagcagt tattgacgaa aattgggatg
    14521 gatctgcttt tgattctttt gaagcacaat tcaatgaatt atctccaaaa attacagaat
    14581 ttgctcaatt actcgaagat attaaccagc aattattaaa agttgcagat atcattgagc
    14641 agacggatgc agatattgct tctcaaatta gtggatagta tcgtatcata gtctataagt
    14701 gaataggaag gcaaagcctt cctattttgt tgaaaataaa atatatttta ggaagaatta
    14761 tgaaaagaaa taactttaga atactttggt atattatagc tgttgctctc tttttagtag
    14821 ctattgctgg ccttaatctt aagcttcaag gcgatcatgc aaaagagaat aaaacaacac
    14881 agtcagctac taacactaaa ctaaatatag cattagttaa cgaagatcaa aatgtatcta
    14941 atggtaaaga aagttataat ttaggagcta gttatattaa gtctatcgaa cgtgataata
    15001 gtcaaaattg gtcagttgtt agtcgaggta ctgctcaaaa tggattaaac aaaggtgatt
    15061 atcaattaat ggttatcatc cctaataact tttctcaaaa actccttgat gtcaataaag
    15121 caaatgcaga gcaaacaact atctcttata aggttaatgc caaggggaat ttagcattag
    15181 agaaaaaggc aactgaaaaa gggaaagata ttgtttcaga gttaaatagc cacttagtaa
    15241 atatgtatat ggcaagtatt ttaagtaact tatatacagc tcaagaaaat gtacaggcca
    15301 tggtaaatgt tcagtcaggt aatatctcaa attaccaaaa aaatctttta gattctgcaa
    15361 ctaatttcca aaatatcttt ccagcccttg ttaaccagtc tagtagttcc attactgcta
    15421 acgaatcact gaaaaaatct ttagaagctt ctgataacat gtttaatgat ttggtgacaa
    15481 cccagacaaa tactggaaaa gatttatcaa gcttaataga acagcgccat caagatagca
    15541 tttcgtatga agcgttttcg gcctcattac tagaaatgaa taacgagtta ttagagaagc
    15601 aattgtctga tattatcaca caagcacaaa aagaccaaga aacattatca tcacaactca
    15661 atagtattat gggtgatgat aacgataacg atcataatca taaagaaaac tcatcagctt
    15721 atctaaatgt tgcaaggcaa aaaatccaag aactatctga agcactcaag tcacaagata
    15781 acgtcgctaa agatcaaagt gaacaactag ataaaattgt tagagaggga ctagcaagtt
    15841 actttgctaa gaataataaa gataatatta ctttattaga attattgaag agtcattcta
    15901 ataatgagaa gactttgaaa gattttaaag ctaaggtagc agatttcaca aattctctca
    15961 tttcaagcat tccttcacta aacctttcgg agttgcactt aacccaagaa gaagaaaaag
    16021 ccattcagtt tacatcttct gattcggaaa tcattaaaaa agtaagcaac gaaagaactc
    16081 tgtatttaaa tactaattta ttgaatcgtc tttttgaagc taggaaaaat agagatgaag
    16141 ctaaaaataa agttaatcaa ttaaaattgc catctagttc tactcgaact ggtgagcaga
    16201 tagtttctgt tgaatcaaat aatccagatt acagggtaga tacctggacg gttaatggta
    16261 agcaaacaag aaccttggac cccagccaaa acaataatat tattatcaat agcagttatc
    16321 aaggggaatc atctaactct agtattacaa aaccaagcta tacaatcact atcgggaatc
    16381 agaaacaaat tgtacaaaat gatgacggta aaacccaacg gtcttacttg gagacagaag
    16441 caacgtatca acgaactcta caagaagtaa ttgatgcata taacaccact cacaatttag
    16501 tatctaaata ctacattttt tctgatggcg aagaaccgca aagtttattt gatcaatttt
    16561 taaatcaaag tgttaacgat acgatggttg atctggtcaa aaatggtata actaagtatt
    16621 tgatggatga aaacactgct gatgcgcaac agaaagttaa agatgtcatg gaggaagttg
    16681 aaaatagcca agatgaattg gcggatcaaa tggcaaaagt aactgagaca aatgtacgac
    16741 taacagatac aatcaaaaaa cagcttgaga cccttcaatc aattaacatg aaggtccaaa
    16801 acatcacaca ggatcaatct aaagtaaacg actcacagaa aacaactgat caacaattat
    16861 ctgatttgaa gaaccagcta gatgggctaa tgacctctgc aactggcgta aaagatatgt
    16921 ccaaatctaa tagtcaagag gcggaccaag ttaatcaaat attcacatca ttcaataaag
    16981 atgttcaaga tgctaaaaat tcgggcaaca aactttcgac ggatgcaaca gatttaatgg
    17041 ctaacttcca aaaagaattg gccaataacg gtgatttcgt agcttctttc tctaaagttt
    17101 ttaatactgc ttataaaaat ggagtgccaa atgatattct ccttaatttc ttatcacgac
    17161 ctgtcgctga atcagcgtct gcagttaggg caactgaaaa tacttatcgc ccattcactt
    17221 ggatactatt attagaagtt gttagcctat ttacagcata tatctttgcg actcaaaacc
    17281 ttattaagaa attgacagat aggtataatg tcaatcgctg gcttcaaaca gactttttaa
    17341 atgtcattgt catttcaggc ctctctcttg tgattggttt agcactggga gttatctcaa
    17401 gcagaagtct tcatgtcatg cctgaatatg taccatcttg gttcctagtt atgactctgt
    17461 ttagtttttt attaattcat agtcagtatt tctttattaa aaattttaaa gcagttggta
    17521 tgggtttagc tttgtttatg attattagtt ttgtttattt atcaaatgcc gtaggcacag
    17581 tagcgactgt tagtggcctt ccaaaattac taaaagctat taatccacta tctatccttg
    17641 aaaatcaatt atcatcttat tttgataatg tgacaactgg atttattttc cttatcttag
    17701 tacttcttgt ggatgttgct tttattatca tgaatatttt tatcacctta aattttgaag
    17761 ctaaagtaaa agagtgaggt tgttgatgaa gttgaaacga tttttagcct gtctactaat
    17821 attatcctgt atgagtcaaa catcagtatt ttctgatggg ttaaagaata atgatattca
    17881 ttttgataca aaacgtttag aatcagattc acaaaaaaat tccggttttc aaactgaatt
    17941 aactaaagat ttatttaaaa aagaagacct taaaaaattg gatgaaaacc gtcaatctaa
    18001 agtctcactc cagaaaaagc agtctaagca actgtttcaa tattcaaata aaggacaatc
    18061 tgataggaaa aaacgtatca aacaattatt ttctgaagaa agtaatcaat ttaaatttgt
    18121 tacaagaaca gaagaaccga ctgtttcggc tgatattggt catatgatta tgtacctggt
    18181 tctcattagt ttggtactaa tattagcttc tataatgact tttaaaataa aggacaaaaa
    18241 ggatgaatac acatattaac attactctag atatggggga aacctcaaaa gatttacgta
    18301 tccctttgaa aattagtgtt aagcaattag taatggaatt agatagtatt tttagccgta
    18361 ctgaaaggcg taccaaatat caactaagag ttataaataa aggtatttta ttgggagaga
    18421 acgatatatt agcgcaccat ccagtaacaa ctggtgatcg tataaggata gaggagtttt
    18481 attaatggaa acagattcgt ttgtatttga agaacaagac tttcaatttg aaaaaggacc
    18541 agatttttgg cagttatctc ttaagcgttc aaaagtagga actcaaaatc tgcaacaact
    18601 gcaattgtta gaaattcacc attctttgtt gatgcctatg acaactgcaa ttgatgcaga
    18661 taccattcaa tttcaattcc agacagaagc gcacgctcta tcttttgaaa gctttaaaaa
    18721 agaaacttta tctgaaaaat tacgattagc cttgaatgtc ttggatttag ataaggcttt
    18781 gtctttgcca gtaaatttca ttttgcatcc ctctaatcta tttttaacaa aaaatgcaac
    18841 tgctaaaata gcttatcgta gccttcctgg gattatgaca cctgagaagt ttggtccaga
    18901 ggagttttta tatcaattca aatgttttgt ctttgcatta ctgacgcaac atgactatat
    18961 agaattgtat aatggtgcta tttctgttat tgaagtatca gatttcctaa aaagcattta
    19021 tcatgcagaa actattcagg ctgttagaga catcattact actgattatg agcagcaagt
    19081 agaagtagaa actcatacgt tagcaaaagt atcaaaggca aaatataagc tttataaata
    19141 cataagtgtc tggctaggag ctttatctac aatactcttg attccgctag tatatctggt
    19201 ttttattcac aatccattca aagaaaaaat gttggcagcg gatacttcat ttattaaagt
    19261 tgattataat caagtgatta atcgactaga acatgtaaaa gtaagtaagt taccttatac
    19321 acagaagtac gagttggcct attcctatat taatggaatg tcattttctg aagaacagcg
    19381 tgaagttatt ttaaacaatg ttacgctcaa gactgatgag ctctatcttg attattggat
    19441 taatattggc cgtggtttag atgatgatgc tattgatgct gccaaacgtt tagacgattc
    19501 tgaccttgtt atttatgcta ttgttcagaa aatggatcag gttagaaaag acaatagttt
    19561 atctggtaaa gatcgtgaac aaaaactttc tgagctacaa acagactatg ataaatattg
    19621 gaaagaccgt aagacagctt taacagatga ggaatccaaa tcgaaaaata gcaataatca
    19681 ttcgacaaat tccaacaaag agttgtcatc tgaaccatca agtactacaa caagtacatc
    19741 ttctaaaact aaaagtaggt aggtggatat gttagttatt tgttattatc aatcactccg
    19801 ctacgaattt aatattgaag aggagaaatc ctttctaata tcatcaaacg gcaagtcgcc
    19861 aataccagta tcagaattag aaaatgacat cacgctaaaa aatatgcaag gtcaattggt
    19921 atatattatt gaccaaaaag aaaaagagtt aactaatggt gtagagattt caggaattgt
    19981 attctattta gcgaataatc aaaaagaaat ctatactccc ttagattacg aagatatatt
    20041 aatcggtgat aaagaagggt atcatatccg ttttaaagaa ggagccccta atttattact
    20101 gaaaaaaata gaaagtaatt ggcagcttaa tttatttgaa ggtgatattt atttgaataa
    20161 tcatcttcaa aaagtagtgc agcagttacc tttaagttta ggtgatgaga ttagcttcca
    20221 aggcataatt attaagcttt tcccagatga aattcaaatc tgggggggaa ctgattatga
    20281 aacatcatta actaagaaag ttatgtcagc ttatcaattt tacgcaggct atcctgattt
    20341 tcaccgctca cctcgtatta tttatcgttc cagtgaagat aaaattacag ttaatgcgcc
    20401 tggtaatgaa cctaataagt ctaaagatga actgttaaaa ttaattgttc ctcctctagt
    20461 gatgattgga gtcagcattc ttatttctat ttttcgtcct cgtggtatct atatcattgc
    20521 aacaatgtca atggctctag tgacgatgat tttctctatc actggttact ttaaaaatcg
    20581 taaacagtat aagcaggact tacaagaacg cattgattcc tatcatgact atttgtctga
    20641 taagtcaatt gaacttcaga agttagctaa agagcaaaag cgtgggcaac actatcatta
    20701 cccaacgatt gaaggtttac aggaaatggc tgatacttat caccatcgta tttatgaaaa
    20761 gacaccactt cattttgact tcttgtatta tcgcctaggt ttgggagaag taccaacgag
    20821 ctataatatt cattattctc aaccagaacg ttctgggaaa aaagatcctt tagagaacga
    20881 aggctataac ctctacttta acaatcgtta tattaaaaat atgccgattg ttgcgaattt
    20941 atcacatgga ccagttggct atataggacc acgaggttta gtattggagc aactccaatt
    21001 gatggttaat caattagctt ttttccattc ttaccatgat gttcaattta ttactatcgt
    21061 tccagaggaa gagatggata agtggtcttg gatgcgttgg ttgccacatg caaccttaca
    21121 agatgtcaat gttcgtggct ttgtttataa tcaaagaagc cgtgatcaag tgctcaacag
    21181 tcttaaccaa attttaaaat tacgtcgcac acaacgcgaa gataaatctg ccaaagaagg
    21241 gactttgttt tctccgcatt atgtcgttat tgtaactgat gaaaagttaa ttttagatca
    21301 cgtcatcatg gaattcttta cggaagatcc tacagaattg ggatgttctc tcatctttgt
    21361 tcaagatgtt atgagttcac tttcagaaaa tatcaaaacc attattaata ttaaagaccg
    21421 taatactggt cagttagtta tagaagaagg agagcttaaa gaaacagatt ttgaattaga
    21481 tcatttctta gaggattatg acaaggaaaa tatttcacgt cgcctagctc cgttaaatca
    21541 cctacaaaat cttaaatctt caataccaga agcagttact tttatggaaa tgtatcaagc
    21601 agaagaattt gaagatttgc atgttcaaga gcgttggata agccatgctc cgtataaaag
    21661 cttagctgtt ccacttggct tacgtggtca ggatgatatt gtttatctaa acctacatga
    21721 aaaagctcat ggtcctcatg gattagttgc aggaacaacg ggatcaggga agtcagaaat
    21781 tatccagtcc tatatccttt ctttagcggt taatttccat ccacacgatg tagctttcct
    21841 tctcatagac tacaaaggtg gggggatggc aaatctcttc aaggacctgc cacacttact
    21901 tggcaccatc accaacttag atggtgccca atcgatgcgt gctttggttt ctattaatgc
    21961 tgaattgaaa cgtcgtcaac gtttatttgc taaagctgat gttaatcata ttaaccagta
    22021 tcagaagaaa tacaaacttg gagaagtttc agaaccaatg cctcatctct tcttaatctc
    22081 cgatgagttt gccgaattga aatccaacca acctgagttt atgaaagagc ttgtctcaac
    22141 agcgcgaatt ggtcgttccc tagggattca cttaatccta gcgacccaaa aaccatctgg
    22201 cgtggtagat gaccaaatct ggtcaaactc acgcttcaaa ctagctctta aagtagcaga
    22261 ccgcggcgac tccatagaaa tgctccatac cccagatgct gccgagatta cacaagcagg
    22321 acgtgcctac ctccaagttg gtaataatga agtctatgaa ctcttccaat cagcatggtc
    22381 aggagcagat taccaacctg aaaaagacga ccaagggatt gaagaccata ccatttattc
    22441 catcaacgac cttggccaat acgaaatcct aaatgatgat ctttcaggct tagaccaagc
    22501 cgaaaatatt aaagaagtac ctacggaatt ggatgccatc gttgaaaaca ttcaagcttt
    22561 aaccaaagaa atgggcatct cagacttacc tcaaccgtgg ctaccacctc taagcaatca
    22621 gattgcagta accgatctcc gtaaggaaga atcagtagac ctctggagta aagctccatc
    22681 ttacaaagct gttctgggct ttatggatat accaagccaa caagcacaag aagtagcttg
    22741 gcacgatttt gaagacgatg ggcatttgtc aatttttgca gggccaagta tggggaaatc
    22801 cacagccctt caaacagtaa caatggatct agcacgtcac aatagtccag aatttctaaa
    22861 cctttatctc tttgattttg gtaccaatgg cctcttacca ttaagacgtt tgccacatgt
    22921 agcagatttc tttaccattg atgatgacga aaaaatagcc aagtttattg cccgtatcaa
    22981 agttgaaatg tctgatcgta agaaagctct cagtcgctac aatgtcgcga cagcaaaact
    23041 ttaccgtcag gtatctgggg aaaccatgcc tcaaatcctg attgttatag acagttatga
    23101 agggctacgg gaagctcaga cactgactaa cttagaagct tgtttccaaa atatttcgcg
    23161 tgatgggtcg tcacttggta tttccttagt tatctcagct ggtcgtatgg cagctttgag
    23221 gtcgtcctta atggcaaacc ttaaagaacg tatcgctctt aaattaacag atgactctga
    23281 atcacggaca cttgtcggtc gtcaccaaca catcatggaa gatattccag gacgtggatt
    23341 aatcaaacgt gatgacattg aagtgttaca agtggccctc ccaacagaag gcactgaaac
    23401 ctttgacatc atcagtaaca tccaaaccga atctgacgcc atgaacgaca aatggactgg
    23461 accaagacca aaagcaatac caattgttcc agaagaatta acctttgacg actttatggc
    23521 tacagatagt gttcaagcag atctatcagc taatcgttta ccacttgggc ttgaaatggt
    23581 tgacgttgaa agctttagcc taccattaac gaaatttaag cacctcttat tcctatcaga
    23641 ttcagatgaa ggtttagaaa atctaggcag tcatttactt aaaacgttga ttaaagtgcc
    23701 agaatacagc accatgatca ttgattcctt aggcgaacat gaagcctacc aaggacaagt
    23761 gagaacctat gtcggtgctg atatgattag tgatatggca gaacaactca attatgagct
    23821 aggaaaacgt caagaacaaa acgctttcga tagatggttt atcttaattc cagattttga
    23881 aagtttcgtc agcaaaacaa accttagcct agagcaaatc caaaatctct tagataatgg
    23941 acccaaagtc ggcttacacc tgatcattgg ctcagccttt agctttgttg gttcaaaact
    24001 tgaccccgtt aacaaatacg tcaaaaccaa tagccaatat gttatgctag gcatgcgcct
    24061 aatggaccaa acattcctag agaaagtgta taatagtaaa gaagcacgtc tggaacgcga
    24121 cgaagcctac attcacgata gaaaaaacta tcaaaaacta aaactcagca tagactagag
    24181 aggagagaga atggcaaaaa tacaaagtga tgcatcactg gcatcaagct gtgccactag
    24241 cattcaaact ggagcaaatg gtattacttc agttggtaaa gcaacaaaag atagcgacag
    24301 tcaatattca ggtcagacga gtgcctcatt atacattgat aatgaggcca gtaaaagtgg
    24361 atcaattgca ggtaaattaa cagaatttat tggtctgatt cattctactg ctagcgaatt
    24421 tgaagctgtc gatcaaaaat tgagtcagtc tatttctaca cctaagcaat acacgactcc
    24481 atctggcaat tcaacgacat ttcaaccaaa accgtatttt aactaggagt ctttatgaat
    24541 gataaacagt taaaagaagt cgaaagaaat gaagccattc ttagaaaaga actggaacgg
    24601 attgaagata agaaaatagt cttaaaaaaa tcctatgaca aaactattaa catgcaactt
    24661 gatattcaac agtctctgag agatagttct caaagtcttt caccagaaga agttatggaa
    24721 caagaagaga tcatgttaat ttttaatcga cagtcaagaa tcgttgaaga ttattttcaa
    24781 gaagaaatgg ctaaactcaa caaacaagaa acagatgcta aagacacatt agaaggactt
    24841 gtccaagagc gacagaaact atatgtgtct caatctgaga agggagaatg atggtaaaag
    24901 ttaatgcagg ttcacttgct gcacaggcgt catccgtatc aacagtagtt gcttcacgtg
    24961 ttacagcttt aaatagtgca aagcaaagtc tgactagttt tgctaatcaa gcaggcttgg
    25021 aaggtactgc ctatcaaaat gcaaaatcct acgccacagg tgtattagga acattaatag
    25081 atggtatgat ccttttatct gaagagacag aggaaggtgt tagtaaacta caaagtttat
    25141 atagcgataa atgtggaggt gaaagtctag attctgacgt cctaaccaaa aaaatcgaaa
    25201 aagatgaaga attattagct aaaattcatg atttgcaagc acacttagac aaacatgcca
    25261 cttttttgtc gcatgccttt gatggattag aagcgacaat aaaagcacgg ctcaaaaaga
    25321 ataagaaaaa attaaaatat ctcatggaat tcaatgcgga atcctcaaca gtctttgatg
    25381 gactaacaga agttcaggat gccgtttcgg caggccttaa ctatgtctca gaaggttttg
    25441 gaaatttcaa tggtacattt gatacctcag gtaaaggtct tgattggaca aaaacaatca
    25501 agactgggat ggaaaagagg agagataagg tttcaattga taaaaattca ttattgaaag
    25561 aagtaaaaaa agcttataaa gctggtaaaa ttaataaaca aacttatcaa tcaataaaaa
    25621 gtgggttagt aaatagttcc actgcttttc tgaaagaatt agctaaagca aaattaaccg
    25681 ataaagcagt ggaaacattc actaaggaat catgggaagc attgtccaag aatttccaaa
    25741 cgatgcagtt ctctggtgtt ttagctgctg taaatggagc tgaattatca actgcaattg
    25801 atgtcaatcc tgcttatgca gagatgattc gtggaggtag tcaatttgtg aaaaaaggag
    25861 ttcctgttat tggtgctgca gttgactttg gactacaact taaagatggt gaagatgttg
    25921 gtgatgcagc agtcaagaca ggtggtcatg ttgcaattgg tattggtgct gcaaaagcag
    25981 gagctataat agggagtgca tttccggtag tcggaactgc tggtggtgcc gttgttggtt
    26041 ttgctattgg ggttgctggt tcaatggctt tcgattggtt gtatgataat aaagaacaag
    26101 tttataatac tactaagaaa gtaatttcca ctcgtaagac tattgattca gtagtttatg
    26161 aaggtttttg gaggtgaaat taatgaataa taaaatttta gcactaggaa cttattactc
    26221 aaatgatatt ttatatgata agcaaacaaa tattttgtat aaggatgtta a
//
LOCUS       DATRSD010000019        25712 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRSD010000019 DATRSD010000000
VERSION     DATRSD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25712
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.019"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..773
                     /locus_tag="VBD85_000638"
     CDS             <1..773
                     /locus_tag="VBD85_000638"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7133205.1"
                     /translation="VLSQSSFYVARAIKKNSCNQKNQLIGYYLDNGEHEYIIPAGIWL
                     DSVGVSIFPEYYEEYLPKIFHQDFSSLSEIVPRIGTDLFIPKISTILDDIATYTPTSE
                     ISELFYEAKILEIIEAFLSWKSNAVHNVIDKEDSEALHRLGHYLEQNFSNPTNIQMYA
                     RMCNMSKSKLSELFHSIYGYTIVEFIKNCRIDKAKRLLLENDLSIQQIASAVGYSHQS
                     SFTYIFKQHVGCTPREYRKDNSNFHSISSTISTSKNHL"
     gene            1048..1407
                     /gene="mobC"
                     /locus_tag="VBD85_000639"
     CDS             1048..1407
                     /gene="mobC"
                     /locus_tag="VBD85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO7133206.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKKRLAKCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATNNINQIAKRVNSTGVIYKEDIGDIKKEIEHFSKELW
                     QIHSLLLKRTSETEGEE"
     gene            1407..2738
                     /locus_tag="VBD85_000640"
     CDS             1407..2738
                     /locus_tag="VBD85_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO7133207.1"
                     /translation="MAITKIHPIKSTLNLAIDYIVNGDKTDEQLLVSTHKCHESTAHT
                     QFLRTRNDAGTKGTVLARHLIQSFLPGETSPELAHQIGMELCKKILKDEYEFVLSTHV
                     DKGHIHNHIIFNNVNMVTGRCYQSNKKSYHQIRYQSDKLCKENSLSVIDEFYESYKKK
                     YKINGKSWYENEQAKHGTSWKSRLQFDIDRMIKQSKDWDEFLKKMADLGYEIKYGKHI
                     AFKPKDKARFTRTKTIGEDYTEERLKERIAEREFIKTPDVKKRIGNVIDMNTNAKVKE
                     SKGYEYWATKHNLHTMAESVIYIREHGIKSVKQLDEYIQKAADERQNIQEKIKAIDKE
                     MQKLSTTMEQVHTVKKYRACYKEYTANPSDKAFFEEYKAQITLYENALSELKKSYSKL
                     PNSKDILAELDKLQEKKNTLMQEYSSSKSTMDELYKIRKNYGIYMGKEMER"
     gene            complement(2807..3466)
                     /locus_tag="VBD85_000641"
     CDS             complement(2807..3466)
                     /locus_tag="VBD85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7133208.1"
                     /translation="MDYKTMRNQIEDMVNDNHKDFVKAVISMEKGINDESALDKLYEA
                     YMDNDSLNLLHEEFDYMIEDLREQGQIKDLPYVQEEKDNLVNIVGNIVGKIDVVEREN
                     KNGEAFQVVNFSVASKDDEGNKVYHNCSAYGEKGDIPKDFKQGDFVKLFGQIRTSVDD
                     NGKEHTNIRILSSKLLKAKEQMKGRDEKKESVLGAIKKYQAEDKEKPKEKKEVSKETE
                     R"
     gene            complement(3507..12224)
                     /locus_tag="VBD85_000642"
     CDS             complement(3507..12224)
                     /locus_tag="VBD85_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO7133209.1"
                     /translation="MRINDFHNILELIKQDVLHSEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEAIACAKFDYWRERFNRTVMRGQKGIPILEDYGTFKKVDYIFDIGQTVSRNR
                     DVNEVNLWKFDKENHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYSIDYELLRENFQRLDSISLMSLGESVSDI
                     SGKIIDATIQKSKELELQKEVLRGKEAGYNKIKEELEEVEEYVLRRDDQGRNENERVL
                     RNGEYRRDNRENQGEYAKQLGGRDGLHKEVSKSDLRSDEAGVSFTERGAEPLRDVSRS
                     IQGEEVDRTPDGHSETGDRIYENRETETDGSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GNLKENTEAEIREAEKASFSLPENSYGQISLTIPLTENDIDTVLINGGNHDGGRLPVI
                     AEFSKEKTVEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQVL
                     NWNDAAMRINELLNSGEFATNAELLEALDYERDRISESLWYLSHDLSEEGKEQGYFEL
                     FERGGGFSEETKRLSEALKNPEYLKETIREYDRLLEGYKENRNVLRFHYHKVDSLYQK
                     LQELELPRKEYSTNLAELPKVKSFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHAVSGAMGSDEWHDAKGLKLQKNDCNDVFLTWTSVAKHIDEL
                     FSKNLYLEEKETENKSEIKEPQYYSKDDPENLMTDEMLERVPELYAQEDVALADKEVH
                     TAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILEDFP
                     KTFRELKDSELKKQMDEQELQSVFNGELSFVEEELEAPEETEEVRRVDTVQATLFDYL
                     KDREEVELNEKEGNHLEDFAVKEGNTVYFNHEEYTIREISKNEITGRNDLWLDPVRSG
                     NHQIPIVTFDDNEDLLEQVSLERPNFIVGDEIRYKDKDYTITRFDDMGNNLKTVTVKD
                     NTEYLGGMITGSDVIPYRLESDLERIFENLTYQSPEKTTEEIEIKKAEAHNFQIKEET
                     LPDKLSPSERLNNNLEAISMLNRIERGERDLDITAQEVLARYVGWGGLADVFDEEKGG
                     QWKEARSFLKENLSQAEYEAARESTLTSFYTPKTVIDGVYKTLSDMGFKQGNILEPSM
                     GIGNFIGNIHDEMNKSKFYGVELDSVSGRIGKLLYPESDIQIKGLEETSFSNNFFDAI
                     IGNVPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKDESVR
                     RYLAARAEFLGAIRLPNDTFKGVAGTEVTSDIIFLKKRDSIRERDEDWIHLAEDENGL
                     LYNKYFVNHPEQVLGSMEEISGRFGNTIACLPKENTDLKELLTKASEEISKNANYEEI
                     ELLDDEITSIPATDDVKNFSYTIIDDEVYYRENSLFVKKEVTDKNKEKIKDYIELNAA
                     LKDVISKQKEDFSDDEVKKAQEKLNEIYDSFSKKHGYVNNLSNTRALKEDSNFPLVSS
                     IEILDEEENFKAKGDIFSKRTITKAKVMDHVDTSLEALVLSVSEKGYVDFEYMGSLTD
                     KDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVTKDEY
                     LSGNIRDKIAIVDSYLAKLRQTERELPHLGYAEDGKEKELISYEMNRLEYQKAELTKV
                     LPKELEASEINVRLGATWIPNKDIEKFIFETLKTPGYAKWDIKVKFSNLTSEWNVEGK
                     SRDRGNDLAEMTYGTSRVNAYKLIEDALNLKETKVFDQIVNSDGSKTSVLNKKETLLA
                     GQKQELLKEEFKNWIFNDQERRNRLVKIYNERFNSIRNREYDGSNLSFEGMTTEIDLR
                     PHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHLTGQI
                     GREFMQLYPSANIMVADKKDFEPKNRKRFIGRIATGEYDAVVIGHTQFEKIPMSKEYQ
                     EKHIQDQIDEIINYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDVITFE
                     ELGVDKLFIDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGGKGIV
                     FATGTPVSNSMTELYTMQRYLQYESLKKNNLEHFDSWASTFGETQSAFELSPEGTGYR
                     VKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEAHYEVIKTLPSEEQKEILKSLS
                     ERADDVRNRVVEPDEDNMLKITNDGKKLALDQRLINPLLPDNLDSKVNVCVKNVFAIW
                     DKTRENKSTQLLFSDMSTPKGNGEFNIYDDVREKLVAMGIPKEEIAFIHEANSDKQKD
                     ELFAKVRKGEIRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIVRQGN
                     ENKEVSIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNYAEIK
                     ALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKLIEAV
                     KKDIKDVEAKAEGEEKFTSITIGGEKILDKKLAGEKLLEAISKVKINESKVIGKYRNM
                     DLEVSYNFFTNEHNFSLNGAAKHSGELGTSADGNITRLDNALEKMPEKLKRLEEKLFS
                     TKEQLENAKEELQKPFEKADELKNKVLRLAELNKLLDMGEVEEKRNDNPLVEDVKRAI
                     IDFCNREYEENHSYDEFDALYPDLKHIGIAYTDTPDERHGIQFELDLENYTATQFVND
                     VVVSHYDYVKENGSVEKALDVIKFEMENGEFNTFVSVDEEELKQAIGLEIDDEGNFYD
                     PLSKDLDNDGIADRYDNDFKDSDYFESTYDVEDNLHTKEEATQKTEDKPSILGQIRAY
                     QEESKTEEKQTAKEQEYVR"
     gene            complement(12308..13855)
                     /locus_tag="VBD85_000643"
     CDS             complement(12308..13855)
                     /locus_tag="VBD85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEO7133210.1"
                     /translation="MQLLDIKRMAHNILENQNIESSFIEYKKSANFKDKILKTACAFA
                     NNYMNNEIGLLFVGIEEVDDKETGEKAIPKRPIEGIKEAKLEGIENEIKSLLANIHPK
                     INYHIITDQIDDEYYIVVAVESGSNGPFQTSERAEKDKDIRLKAGRYIRVGRDSRLPN
                     PTEEFELLKKFANFSFSSNLNDTATIDDLSYEYMKEYLLQTGAKKDIREMSKLDMAKS
                     MGLVSESEYGGYRAKNFAVLMFADTPNKFIPNAHVEIIREIDGTDKMESKKFDGPVWI
                     QAKQVSKYFEDNIMASYTIREADKIEHKIVYNYPLTAFEELATNAILHKEYDTPEYVG
                     IYIYKDRISFVNHNRPLPPVTIEALNRDRTFDRRQYLNKELKDMFFSLNLIESYGSGI
                     RRAKDALLENGSPELKFYPDNEEDNYTNAVMGVNKEFLKEFNGSTTKETTKETTKETT
                     KEKNSIQEQIVLLMKDNPSITAEQIANEIKEITADGVRYHIRNLKAHGVIKREGSTKS
                     GKWIVLK"
     gene            complement(13937..15649)
                     /locus_tag="VBD85_000644"
     CDS             complement(13937..15649)
                     /locus_tag="VBD85_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEO7133211.1"
                     /translation="MEHILVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGH
                     LIQMANPDAYDEKYAKWNMADLPIIPKDYKYEVAKSTKKQFNILKKLMNDKEVDTVIN
                     ACDAGREGESIFRLVYNQANCKKKMKRLWISSMEDSAIRDGFDNLKDGKDYDNLFESA
                     QARAIADWLVGMNISRLYSCLYKQNYSVGRVQTPTLYMIVKRDEEISNFKKEKYYTVE
                     LSMNGFTLSTDKIGDEITAEQLINLIGDNIEITDVIQKEKITKPDLPFDLTTLQRECN
                     KYFGYSTKQTLDYAQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKT
                     VFNSKKVTDHHAIIPTVSSLSEDLSSIPDSEAKVYRLISNKLHASVGYPLVENTTKIV
                     AEFDGFEFTSSGKVIRDEGFSKYLKEYKSKKNEFIELPDVSIGDVLSIENKEIKEKFT
                     QPPKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKGFVERDKK
                     NLIATHKGISLVTIVADTFKSAETTAKWEMELADIAQGKSSKEKFLKDIESEIQEAVL
                     KYRK"
     gene            complement(15744..17279)
                     /locus_tag="VBD85_000645"
     CDS             complement(15744..17279)
                     /locus_tag="VBD85_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEO7133212.1"
                     /translation="MKTSWKKNKKFWTAALAVVVSISCLLGLWTVVYAHEQSLTEAPT
                     SDKAVQTQVEVNVKYIFEDEKVYKEEKIKAEKGQLLDSGDLPMLPDNMKFIDEFLFYE
                     VKGDGNDEIIRKVAKTEAKDKETQTEDEKPKQDESSQTKTPKTEDKSTQTELSKDDIS
                     KMEKDTKELQEKLDKLNSEMKDKDKLSDNQKEKIKELEAQIESLKEKMKKDKENKDLS
                     EEMKKEMDKLNKKIKEIEEKANEANKAPVISNPSALNSPISGIKTNSGISNQTPVNNT
                     GKSTTTQASSENTVKDTSNSETKEKDIRYPNKLTPKAPANNNQDSSMDGTSKTVNTNK
                     GVASAPSKARGTVTENKDNANKDYPIHHGDSGDNKETDLYSADARQFITFQTKNGKTF
                     HLIINHDETQENVMLLTEVSEDDLLNMVEKKEAPKQEISKEEPTKEEAKPEKKEEKSN
                     LGTYIILLLVIGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSEAESEEEIGEAEVE
                     DEE"
     gene            complement(17254..17493)
                     /locus_tag="VBD85_000646"
     CDS             complement(17254..17493)
                     /locus_tag="VBD85_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7133213.1"
                     /translation="MKRELTVVKNKIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLMAKVNRQRVEMKKEKSNENQLEKE"
     gene            complement(17505..19637)
                     /locus_tag="VBD85_000647"
     CDS             complement(17505..19637)
                     /locus_tag="VBD85_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7133214.1"
                     /translation="MGKKLKKDFRERHKASLEREMLHSETFTTSEESKLKHNDDYRGK
                     IAHDKDRFQDKVHKKTIKVSSDNEKAYGTSKRNARYRASDKDSVAAVSETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSDYFESTYDVDDNLHKKDEFFGSSSITHE
                     AKKRKYKRKNYTESLYTRKKEDVSKENKSEGKKTGKDAASEKVHSSLSKEQRKKLKKD
                     MVKVSALSGLVKGSETVRDYLSHGSDENKGVEAGEKTADASSKLIHGIKKYSDKKKAK
                     KGYDLTNKDYKIRKRKSKLEFRDAKENLKKTNEYKRANAYKRFQKKNQVKAAIRRENK
                     SRLRDRIKENLIGSLKASKRFIVRKAKGLMIIFIGLIILGTFFINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLNEINQNFSAMESELQNEVDHVKNNYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEVEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMGEAFGNS
                     ELISANGGVGGGVEYEASSEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSTAGLTYGHVGIYIGDGKVMDNIGRI
                     RVTTLDNWIASFCKHHPVGFGFPPNVKK"
     gene            complement(19641..22070)
                     /locus_tag="VBD85_000648"
     CDS             complement(19641..22070)
                     /locus_tag="VBD85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7133215.1"
                     /translation="MKDKRKIQQVKLEQNKKKLRSQKQDLKQNKGKSKKDKGGLLDLI
                     FRKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRLEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSNLKSMGVRAESLTGEERLKILHD
                     ILNPNKTFEFSYKDLKKKESTKTYIAPDEFNFTPSRYFKFGKFIGAVSHFQILASELS
                     DRMLAEFLDIDDNINISFHIKAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFAKTVQKLDNTISQISSIANKH
                     NCKLKRLDHAQEQGLISVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFNGEVIKVSSKSKDFLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYERYFENPEPCNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFLDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(21982..22371)
                     /locus_tag="VBD85_000649"
     CDS             complement(21982..22371)
                     /locus_tag="VBD85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEO7133216.1"
                     /translation="MAYVPIPKDLKKVKTKVAFNLTKRQMIGFTLAGLVGLPVYLFMR
                     KVVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     RQKKDSAGKVRTKQKEVKKSKTGLKAK"
     gene            complement(22375..22638)
                     /locus_tag="VBD85_000650"
     CDS             complement(22375..22638)
                     /locus_tag="VBD85_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7133217.1"
                     /translation="MIKRKTVFTAVVIGAGVIAVIDRIKLFSKINDLEERTKDIGRCH
                     NDFCILQERYNKNIDKQIESIQEEIGSVYEHMAELSKEKEDGR"
     gene            complement(22656..23519)
                     /locus_tag="VBD85_000651"
     CDS             complement(22656..23519)
                     /locus_tag="VBD85_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133218.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSATVSGDQIVAMMDTLKEKG
                     LGELVMILFETSLIKVAIEVISIVIMLVVYGRMFEIYVYSSVSAIPFATMGNKEWGQI
                     GTNYIKGLFALGLQGLFLMVCLGIYAVLVKTIQITDIHTSTMTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(23539..23754)
                     /locus_tag="VBD85_000652"
     CDS             complement(23539..23754)
                     /locus_tag="VBD85_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEO7133219.1"
                     /translation="MEFFTQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(23758..24069)
                     /locus_tag="VBD85_000653"
     CDS             complement(23758..24069)
                     /locus_tag="VBD85_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7133220.1"
                     /translation="MKQEMININANLLAEPTFSSFDKEGETVEVANFTLVKKYGKGKE
                     YINCAAYGEKSEKAKDFEKGDLIHIFGYFKKREKEGKTYKNFVVKSYNKIEKKEESEE
                     E"
     gene            24454..25629
                     /locus_tag="VBD85_000654"
     CDS             24454..25629
                     /locus_tag="VBD85_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEO7133221.1"
                     /translation="MFYIGVDIAKKNHEASIIDSDGKLVSESFSFSNSIRGLEKFQKF
                     ISSFSIDSNNCIIGMEATGHYWLSLYSFLIDLGFSCVVINPIQSDAFRKMYIRQTKND
                     SVDSFVIAQILRFGEFSVSHFSDEDTFALRNLSRFRFALVDEASDWKRKLVCILDQVF
                     PEYSSLFSNIYGVASKELLSKYPLPEDMISIPADELAKMLSKCSKGRFGIDKAKEIQE
                     KASNSFGVKFALKSFSFQIKQIIAQISFLEDQIADIEDEISSMINSLCPVITSITGIG
                     DVLGAAIFSEIGDISRFERANQLVAYAGLDVAVKQSGNFNATDTKISKRGSPYLRRAI
                     WLAASVAAFKDPALSVYYQKLRQRGKAHGTAIGAVARKLTNIIFAVLRDNKTYIPNI"
BASE COUNT         6856 a         5439 c         3932 g         9485 t
ORIGIN      
        1 tggtactttc ccaaagttca ttttatgttg caagagcaat taaaaaaaat tcttgtaatc
       61 aaaaaaatca acttattggt tattaccttg ataatggcga gcatgaatat ataattcctg
      121 ctgggatttg gttagacagt gttggagttt caattttccc tgaatattat gaagaatacc
      181 ttccaaaaat atttcatcaa gacttttctt cactttcaga aatagtccct cgaattggta
      241 cagacctatt tataccaaaa atctcaacaa tacttgatga tattgccaca tacacaccaa
      301 catcagaaat ctcggaacta ttctatgagg ctaaaatatt agaaataatc gaagcatttt
      361 tatcttggaa aagcaatgct gttcacaatg ttatagacaa agaagattca gaagcacttc
      421 atcgtcttgg tcactattta gaacaaaact tttccaatcc aacgaacatt caaatgtatg
      481 cacgcatgtg caatatgagc aaatctaaac tgtctgaact atttcactca atatatggat
      541 atacaattgt agaatttatt aagaactgcc gcatagataa agcaaaaaga ttattattag
      601 aaaatgattt atcaatacaa caaatcgcta gcgctgtggg gtattctcat caaagcagtt
      661 ttacttatat tttcaaacaa catgtaggat gcacgccacg tgaatataga aaagataatt
      721 ctaattttca cagcattagt tctacaattt caacttctaa aaaccattta tgatattaac
      781 agttctgtaa cctcaatcat tatctgtatt ttggcaatct ttatatagaa tagcccacaa
      841 ttgtttgtta taatacagtt ataaattaaa aaatttgctt aacgcttaat ctacaaatca
      901 ttcttttgag caagcacagt aagtgtacgg acacttacga aaaaattgac gaagcagccc
      961 ttggggatct gcttcttttt ttatcaattt ttagcttgtt agggagaacc ctaagacccc
     1021 gaaatacatt caaaggagga actaactatg gcaaatagaa taagaaatga aagacttgaa
     1081 attaaactaa ctgaagaaga aaaggctctt tttgaagaga aaaaaagact tgcaaagtgt
     1141 agaaacatga gccatttcat ccgcaaatgc gttttggaaa aggaaattta tcaagtggat
     1201 ttagagcctt tcagagattt acaaggctta ctttctaatg caacaaacaa catcaatcag
     1261 attgcaaagc gagtaaattc gacaggtgta atctacaaag aggacatcgg tgatataaaa
     1321 aaagagattg aacatttctc aaaagagctg tggcaaattc attcactact tctgaaaaga
     1381 acatctgaaa cggaaggtga agaataatgg ctattacaaa aatacaccca ataaaatcga
     1441 ctcttaatct tgctatcgac tacattgtaa atggagataa gacagacgag cagcttttag
     1501 taagcactca taaatgccac gaatcaactg ctcacactca gtttttaagg acacgaaatg
     1561 acgcaggaac aaaaggaacc gttcttgcaa gacatctcat tcaatcattt ttaccgggag
     1621 aaacaagccc tgaattggct caccagattg gtatggagct gtgtaaaaag atactcaaag
     1681 atgagtacga atttgtctta tctactcacg tagataaggg gcatatccac aatcacatca
     1741 tcttcaataa tgtaaatatg gtaacaggta ggtgctacca gtctaacaag aaaagctacc
     1801 accaaatccg ttatcagagt gataaactct gcaaagaaaa tagcctatct gtcattgacg
     1861 agttttacga aagctataag aaaaaatata agattaacgg taaatcttgg tatgaaaacg
     1921 aacaggcaaa acatggcact tcttggaaaa gcaggcttca atttgacatt gacagaatga
     1981 taaaacagtc aaaggactgg gacgaatttt taaagaagat ggctgatctt ggctatgaaa
     2041 tcaaatacgg taagcacatt gcttttaagc caaaagataa ggcgagattt acaaggacta
     2101 aaacaatcgg agaagattat accgaagaaa gattaaaaga acgaattgca gaaagagagt
     2161 ttatcaagac tcccgatgtc aaaaaacgca tcggcaatgt tattgacatg aacaccaatg
     2221 caaaggtaaa ggaaagcaaa ggctacgaat attgggcaac caaacataac cttcatacaa
     2281 tggctgagtc tgttatttat atcagagaac atggcattaa atctgttaaa cagcttgacg
     2341 agtatattca aaaagcagcc gatgaaaggc aaaatataca agagaaaatc aaggctattg
     2401 ataaggaaat gcagaagctt tccaccacta tggagcaagt tcataccgtt aaaaaataca
     2461 gagcgtgcta caaggaatat actgctaatc cgtctgacaa ggcatttttt gaagagtaca
     2521 aagctcagat taccctatat gaaaatgctc tctcagagct taaaaaatcc tattccaagc
     2581 tcccaaattc aaaggatatt ttagctgagc ttgataaatt acaagaaaaa aagaacaccc
     2641 ttatgcaaga gtattcttcc tcaaaatcca ctatggacga gctttataag atacgaaaaa
     2701 attacggaat ctacatgggt aaggagatgg agaggtagtc tttatctcct ttttttccta
     2761 aataaaaaag agtcggtgca gtcctaagac cacaccgacc ataagtttat ctctcagttt
     2821 cttttgatac ttctttcttt tctttaggct tttccttatc ttcagcctga tattttttaa
     2881 tggctccaag cacagactct ttcttttcat ctcgaccttt catctgttct tttgccttta
     2941 agagttttga agaaagtatc ctaatgttag tatgttcctt gccgttatcg tcaacggaag
     3001 ttctaatctg tccaaagagt tttacaaaat ctccctgctt aaagtctttt ggaatatcac
     3061 ccttttctcc gtatgcggag caattatgat acaccttatt cccctcatca tctttagatg
     3121 ccacagagaa atttactacc tgaaaggctt cgccgttctt attttccctt tcaactacat
     3181 cgatctttcc tacaatattt ccaacgatat tgacaagatt atctttctcc tcttgaacat
     3241 aaggaagatc tttaatctgc ccctgttctc ttaaatcttc aatcatgtaa tcaaattcct
     3301 catgcagtaa attaaggctg tcattgtcca tataagcctc atagagctta tccaaagcac
     3361 tttcatcgtt gatacctttt tccatgctga taaccgcctt aacaaagtcc ttgtgattat
     3421 catttaccat atcttctatc tgatttctca ttgttttgta atccatgttt tatctccttt
     3481 catggcaaaa gggaggtttc cctcccttat cgtacatatt cctgttcttt tgcagtttgt
     3541 ttttcttctg ttttactttc ttcctgatag gctctaattt gtcctaaaat agacggctta
     3601 tcctctgttt tctgtgttgc ttcctctttg gtatgcaaat tatcttcaac atcataggtt
     3661 gactcaaaat aatcgctgtc cttaaagtca ttgtcatatc tgtcagcaat tccatcatta
     3721 tcaaggtctt tagaaagtgg atcatagaag ttaccttcat catcaatttc aagtccaatt
     3781 gcttgcttta attcttcttc atctaccgaa acaaaggtat taaactctcc attttccatt
     3841 tcaaatttta tgacatcaag agccttttca acacttccat tttcctttac ataatcataa
     3901 tgacttacta caacatcatt tacaaattga gttgctgtat aattctcaag gtctaactca
     3961 aactgtatgc catgtctttc atccggtgta tcagtataag caattcctat gtgctttaaa
     4021 tcaggatata gagcgtcaaa ctcatcataa ctatgatttt cttcatactc tctgttgcag
     4081 aagtcaatga tggctcgttt tacatcttct acaaggggat tatcatttct cttttcttcc
     4141 acttctccca tatcaaggag cttattcagt tctgccaagc gaagcacctt attctttagt
     4201 tcatcagctt tttcaaaagg cttttgtaat tcctctttgg cattttcaag ctgttctttt
     4261 gtgctgaaga gcttttcttc aagcctcttt aatttctcag gcattttctc aagagcatta
     4321 tcaagccttg taatattacc gtccgcactc gttccaagct ctcctgaatg ctttgcagca
     4381 ccgtttaagc tgaagttatg ctcattggtg aagaagttgt aacttacctc taaatccatg
     4441 tttctatact taccgataac tttgctttca ttgatcttta ctttagaaat agcttcaagc
     4501 agcttttctc cggctaactt tttatctaat atcttctcac caccgatagt aatagaagta
     4561 aacttttcct ctccttcagc ttttgcttct acatctttaa tatctttctt gacagcttct
     4621 atgagttttt ccgttcttgc tatttcttcc gggtagtttt tagcaacctt atcctctaat
     4681 ctgtaacggt tggacttgta gtttgcttca agcattttaa gttttgtaac ctcattatcc
     4741 aaatccatct tttccttgat tttcggatca cctgtagcaa gagctttaat ctctgcatag
     4801 tttaagctgc tttcgtccac atcttccgcc actctgacag gtgttttact tgtcataatc
     4861 tgagaaatga acttctgttt attctcgatg gtctgccaaa ggtacgcatc aaaggtattc
     4921 tccgttacat atctgtatat gctgacttct ttgttttcat ttccctgtct tacaattctt
     4981 cctgctctct gctcaaggtc ggcaggacgc catgggacgt ctaaatcatg aagtgcaatc
     5041 agcttgtttt gcacattcgt tccggctccc attttctgtg tggaacccat taaaatacga
     5101 atctcacctt tccttacctt tgcaaagagt tcatcctttt gcttatcgga atttgcttca
     5161 tggataaagg ctatttcttc tttcggtatt cccattgcca caagtttttc tctaacatca
     5221 tcgtaaatat taaactctcc atttcctttt ggagttgaca tatcggagaa aagaagctgt
     5281 gtggacttat tttctcttgt cttatcccaa atggcaaaaa catttttcac gcacacattg
     5341 accttgctgt caagattatc cggaagcagt ggattgatta aacgctgatc taaggcgagt
     5401 ttcttgccgt cattagtaat tttaagcata ttatcttcgt caggctctac taccctgttt
     5461 ctcacatcat cagctctttc cgataagctc tttaggattt ccttttgctc ctcacttggt
     5521 aaagttttaa taacttcata gtgtgcttca ggtgttggaa gatttagcat atctgctgtc
     5581 tgaatatccg caacttcttt aaacatagac attagttcag gaaggttata gaacttggaa
     5641 aatcttgtct ttactctata ccctgtacct tcaggagata attcaaatgc tgactgcgtt
     5701 tcaccaaagg tagaagccca gctatcaaaa tgctccaaat tattcttttt aaggctttca
     5761 tactgaagat aacgctgcat agtataaagc tctgtcatcg agttactgac aggcgttcct
     5821 gtggcaaaga caattccttt tccacctgtc atttcatcca tgtatctgca cttcataaac
     5881 atatcggagg acttaaaggc ttcagactgc ccaatacctg ctacatttct catttttgta
     5941 taaaggtaga gatttttgta attatgtgcc tcgtcaatga agagcttatc tacacctaat
     6001 tcttcaaaag taatgacatc atctttttta aaatcatcgt ttaacttttc aagccttgtt
     6061 tcaagtttct tctttgtctt ttcaagttgc tttacggtaa aattctgatt tctgtcatgc
     6121 ttatattcct ccacatagtt tataatttca tcaatctgat cttgaatatg cttttcctga
     6181 tattccttac tcatagggat cttttcaaac tgtgtatgcc cgattacaac ggcatcatac
     6241 tctcctgtag caattcttcc gataaatctt tttctgtttt ttggctcaaa gtctttctta
     6301 tctgcaacca taatgttagc tgacggatat agctgcataa actcacgacc gatttgacct
     6361 gttaagtgat tcggaacaac aaacaaggac ttactgcaca ttccaagcct tttactttcc
     6421 atcgcagacg ccaccatttc aaaggtctta ccgcttccta ccacatgggc aagcaaggta
     6481 tttcctccat aaaggcttct tgctatggca tttctttgat gaggtcttaa atcaatttct
     6541 gtggtcattc cctcaaaaga aaggttgctt ccgtcatatt ctctgttgcg gatagagtta
     6601 aaacgctcgt tatatatctt tacaagacgg tttcttcttt cctgatcgtt aaatatccag
     6661 ttcttaaatt cttcttttag aagctcctgt ttctgtcctg caagaagcgt ttccttttta
     6721 tttaatacag aagttttcga gccgtccgaa tttacaatct ggtcaaatac ctttgtttct
     6781 tttaaattta gagcatcttc aatcagttta taggcattta cccttgaggt gccgtaggtc
     6841 atctctgcaa ggtcatttcc tctgtccctg ctctttcctt caacattcca ttcacttgtc
     6901 agatttgaaa atttaacctt aatatcccat ttggcatatc ccggagtttt tagcgtttca
     6961 aagatgaatt tttcaatatc cttattcggt atccaagtag ctccgagtct tacattgatt
     7021 tcacttgctt caagctcctt tggaagaact tttgtaagct ctgccttttg atattccaaa
     7081 cggttcatct catagcttat cagctctttt tctttgccat cttccgcata gccaagatga
     7141 ggtaactctc tttcggtttg tcttaacttt gcaaggtaac tgtctactat ggcaattttg
     7201 tccctgatat ttccgcttag atactcatcc tttgttacat agccgtactt atatgaatta
     7261 ctgccgtttg cacaggcaaa aggtagatct ccatcttcaa ggttaaaaga taatggtcta
     7321 taaaagtttt gttcttctct gatgtttaga tagatttccc ctctaagttc ttctatcaaa
     7381 gtcggtctgt ctttatctgt aaggcttccc atatactcaa aatccacata tcctttttca
     7441 gataccgata aaacaagagc ttcaagggaa gtgtctacat ggtccataac cttcgccttt
     7501 gtgattgttc gcttggagaa aatatctccc tttgctttga agttttcttc ttcgtcaagg
     7561 atttcaatgg aagaaacaag cgggaagttg ctatcctctt ttaaggctct ggtattagaa
     7621 aggttattta catatccatg cttcttagaa aagctgtcat aaatttcatt tagtttttcc
     7681 tgagctttct ttacttcatc atctgaaaaa tcttctttct gtttggaaat aacatccttt
     7741 aaggctgcat ttaactcaat atagtcctta atcttttcct tgtttttatc cgttacttct
     7801 ttctttacaa acagggaatt ttctctgtaa taaacttcat catcaattat ggtatatgaa
     7861 aagtttttaa catcatctgt ggcaggaatt gaggtgattt catcatcaag tagctctatt
     7921 tcttcataat tagcattttt agagatttct tcgcttgctt ttgtcagtag ctcctttaag
     7981 tctgtgtttt cttttggcag gcaagctatt gtattcccaa atcttccgga tatttcttcc
     8041 atagaaccaa gcacctgttc aggatgattg acaaagtatt tgttatataa aagcccgttt
     8101 tcatcttcag caaggtgaat ccagtcctca tccctttctc tgatactgtc ccttttcttt
     8161 aggaagataa tatctgaggt tacttctgtt cctgctacgc ccttaaaggt atcgttcgga
     8221 agtcttatcg ctcctaaaaa ctctgctctt gctgcaaggt agcgtcttac actttcgtcc
     8281 tttttatcca tcgttccact tgatgtaata aaggcgataa taccgccgtt tcttacttta
     8341 tcaatggact tggcaaagaa ataatcatgg ataaggaagt tatttttgtt atactccctg
     8401 tcatttacct tgtattctcc aaagggtaca ttgccgataa tcgcatcaaa gaagttattt
     8461 gagaaagaag tttcttcaag ccccttaatc tgtatatcac tttcagggta taacagcttt
     8521 ccaattcgac cgcttacaga gtcaagctct acaccataaa acttagactt attcatttca
     8581 tcatggatat tgcctataaa gttccctatt cccatacttg gctctaaaat gtttccctgt
     8641 ttaaatccca tatccgaaag cgtcttatac actccgtcaa tcactgtttt cggtgtataa
     8701 aagctcgtta aagtagattc tcttgcagct tcatattctg cctgagataa gttctctttt
     8761 agaaagctcc tcgcttcttt ccactgtccg cctttttctt catcgaatac atcagcaagt
     8821 cctccccagc ctacatacct tgctaaaact tcctgagcgg taatatctaa atctctctca
     8881 cctctttcaa ttcgatttaa catcgagatt gcttcaaggt tattgtttaa tctctcactt
     8941 ggagataact tgtcaggtag cgtttcttct ttaatttgga agttatgagc ctcagctttc
     9001 tttatttcaa tttcttcagt tgtcttttca ggactctgat atgtcagatt ttcaaatatc
     9061 ctctcaaggt cgctttcaag acgataagga atcacatcgg agcctgttat cataccgcca
     9121 agatattctg tattgtcctt aaccgttacc gtctttagat tatttcccat atcatcaaag
     9181 cgtgtaatgg tatagtcttt atccttgtat ctgatttcat ctccaacgat aaagtttggt
     9241 ctttcaaggc ttacctgctc taataagtcc tcattatcat caaatgttac aatcggtatt
     9301 tgatgattac cacttcttac cggatcaagc cataagtcat ttcttcctgt gatttcattt
     9361 ttagaaatct cccttatagt atattcttca tggttgaaat agacggtatt accctcttta
     9421 actgcaaaat cctctaaatg gtttccctct ttttcattga gctctacttc ttctctatcc
     9481 tttaggtaat caaataaggt agcttgaact gtgtcaaccc ttctaacttc ttctgtttct
     9541 tcaggtgctt caagttcttc ttctacaaag ctaagttctc cattaaagac agactgaagc
     9601 tcctgctcat ccatctgctt tttaagttca ctgtctttaa gctctctaaa ggtctttggg
     9661 aaatcctcta aaataagcct ttgggcattt agttccttca gctcttcaag attaaaatat
     9721 ccccattcag gctcaatccc aagaacaagt ccaaaggcat caccgctttc tctatcatat
     9781 tccgtcatat accaagtcca atttgaacgg aaaggaatga tatatgcagt atgaacctct
     9841 ttatccgcta aagctacatc ctcctgtgcg taaagctccg gcactctttc aagcatttca
     9901 tctgtcatta agttttcagg atcatctttg gaataatatt gtggttcttt aatctctgac
     9961 ttgttttctg tttccttttc ttcaagataa agatttttag aaaacaactc gtcgatatgc
    10021 tttgcaacac ttgtccaagt aagaaacaca tcattacaat catttttctg taatttaagt
    10081 cccttagcat cgtgccattc atcacttccc attgcaccgg aaacagcatg ggaatgtcct
    10141 ccaattccat attcgtcttt taagaaattc gctttttcct gaagcgtatg attttcttta
    10201 aaaaacttgg tgattcgttc ttttcctcta tcaacgccac ttcctcttga aagactttca
    10261 aggacttcat cttctgtaat aaaggacttt accttcggaa gttctgccaa attggtgcta
    10321 tattcctttc gtggtagctc aagttcttgt aatttctgat aaaggctatc taccttgtga
    10381 tagtgaaacc ttagtacatt cctattttct ttgtatcctt ctaaaagtct gtcatattct
    10441 ctgattgttt ctttcagata ctcaggattt tttaatgctt cagataatct ttttgtttct
    10501 tccgaaaagc ccccgcctct ttcaaaaagt tcaaaatatc cttgctcttt tccttcttca
    10561 cttaaatcat gcgatagata ccatagagat tctgaaatcc gatctctttc ataatcaaga
    10621 gcttctaaaa gctctgcatt tgtggcaaat tccccactat taagaagttc atttatcctc
    10681 attgcagcat cattccagtt taagacctgc gtattatctt ctcttgccga tgttccatag
    10741 gctaagtgaa tgcctttatc cgaataccag gaagatactt ctctttcatc aatgtaaaat
    10801 ccgtttccgc ctttaaaggt atctttgaga tattctccca attcttctac tgttttttct
    10861 ttggaaaact cagcaataac aggaagtcta ccgccatcgt ggtttcctcc gttaataagg
    10921 acggtatcta tatcattctc agttagcgga atcgtaaggc ttatctgtcc ataggagttt
    10981 tcgggtaaag aaaaagaagc cttttcagct tctcttatct ctgcctcagt attttctttt
    11041 agatttccac tacttccttg atggtcattt ctttgagagc tgaaatcatc gccccatact
    11101 gcggattgtt ctcgtcctct atcttccaag ctgccatcag tttcggtttc tctgttctca
    11161 taaattctat cgcctgtttc tgaatgtcca tcaggtgtcc tgtcaacttc ttctccttgt
    11221 atagatctac taacatctcg aagtggctct gctcctcgct ctgtgaaaga aactccagcc
    11281 tcatcactgc gtaggtcgga tttggatact tccttatgaa gtccgtctct tcctccaagc
    11341 tgtttagcgt attctccttg attttctcta ttatctcgtc tgtactctcc atttcggaga
    11401 actcgctcgt tttcatttct tccctgatca tctcgtcgaa gtacatattc ttctacctcc
    11461 tctaattctt ctttaatctt attatatccc gcttcttttc ctcttaaaac ttctttttga
    11521 agctcaagtt ctttgctttt ttgaatggtc gcatcaataa tctttccgct aatatccgat
    11581 acgctttcac caagactcat tagagatatg ctgtcaagtc tttgaaaatt ctctcttaaa
    11641 agctcgtaat ctatcgaata atctaactta aatcttgacg ctaccgcata gcttaccgag
    11701 tcccttacaa acttggtaaa ggatattcta tcctcatccg ctactctaag ttcattcatt
    11761 aaggtatcta ttttttcatc accgtagagt ctgcttaaag aaaagatgtt ttcaagtgtg
    11821 ctttcacttt cctcatagcc ctcgcttttt atcatttcct ttaacacatc ttgatgattt
    11881 tctttgtcaa acttccaaag atttacttcg ttgacatctc tgtttcttga aactgtctga
    11941 cctatatcaa aaatatagtc cacttttttg aatgtgccat agtcctctaa aatggggatc
    12001 cctttctgtc ctcgcattac tgttcgatta aaacgttccc tccagtagtc aaacttggca
    12061 caagctattg cttcaggatt tttatcatag atacttaatt gactcctaaa gtcatatctt
    12121 tgattgtttc cgacaacctt taagagcttc agatactctg cttcactatg aagcacatct
    12181 tgttttataa gctctaaaat gttatgaaaa tcatttattc gcatttttac ctccttcgtt
    12241 tttccaaaca aaaaaaggcg gttagatttt actctaatca ccttttggtt ctatgatctt
    12301 atttattcta tttcagcact atccatttac ctgatttggt agagccttct cttttaataa
    12361 ctccatgagc tttaagattt cttatatggt atcgcactcc atctgctgtt atttctttaa
    12421 tctcatttgc tatctgttcc gcagtaatac ttggattgtc tttcataaga agcactatct
    12481 gttcttggat gctgtttttt tctttggtag tttctttggt agtttctttg gtagtttctt
    12541 tggtagtgct accattaaac tctttcaaaa attctttatt cacacccata actgcgttgg
    12601 tatagttatc ttcttcatta tccggataaa acttaagctc aggagaacca ttttctaata
    12661 gtgcatcctt tgcacgcctt atacccgaac cgtaagactc tatcaaatta agtgaaaaga
    12721 acatatcttt aagttctttg ttaagatact gccttcgatc aaaagttcta tccctgttaa
    12781 gagcctcaat ggttacagga gggagcggtc tattatgatt cacaaaagaa attctgtcct
    12841 tgtaaatgta tatcccaaca tattcaggcg tatcgtattc cttatgcaaa atggcatttg
    12901 tagcaagttc ctcaaatgct gtaagaggat agttatagac aatcttatgc tcgattttat
    12961 ccgcttccct tatggtatat gaagccataa tattatcttc aaaatatttg ctgacttgct
    13021 ttgcctgtat ccaaaccgga ccatcaaatt tctttgattc cattttatca gttccatcaa
    13081 tttctcttat gatttcaaca tgggcatttg gaataaattt atttggtgta tctgcaaaca
    13141 tcagcacagc aaaattttta gctctataac cgccatactc actttcactg acaagcccca
    13201 tactctttgc catatccagc ttagacattt ctctaatatc tttttttgct cctgtttgaa
    13261 gcaaatattc tttcatatat tcatagctta aatcatctat ggtagctgta tcgttcaagt
    13321 ttgagctaaa agaaaagttt gcaaacttct tcaaaagttc aaattcttcg gttggatttg
    13381 gaagccttga atctctaccg actctaatat atcttcctgc ctttaatcga atatctttat
    13441 ctttttctgc cctttcactt gtttgaaacg gaccgttact accactttct accgcaacta
    13501 cgatatagta ttcatcatca atttgatccg taataatgtg atagttgatt ttaggatgaa
    13561 tattggcaag gagcgattta atttcatttt ctattccctc taattttgct tcctttatcc
    13621 cttcaatcgg tcttttagga attgcttttt ctcccgtttc tttatcatcg acttcttcaa
    13681 taccaacaaa caacaagcct atctcattgt tcatatagtt attggcaaag gcacaagcag
    13741 tttttagaat tttatcttta aaatttgccg actttttata ttcgataaaa ctgctttcta
    13801 tgttttgatt ttcaagaatg ttatgagcca ttctttttat atctaaaagc tgcataatac
    13861 ctcctctttt caaaattttt atctgtatat tataccattt ccagaggatt ttctccactc
    13921 tgaaatcagc aggcacttac ttacgatatt tcaaaaccgc ctcctgtatc tcgctttcaa
    13981 tatctttcaa gaatttttct ttggaagatt tcccttgggc aatatccgct aattccattt
    14041 cccactttgc tgtcgtttct gccgatttaa aagtatctgc tacaatcgtt acaagactga
    14101 ttcctttatg tgtagcaatt agatttttct tatctctttc gacaaatccc ttaaagatta
    14161 gattttcaat aattcctgcc cttgttgccg gagtgccaag accttttctt tccacttcta
    14221 cacctttttc taaggcatca tttcctgcaa tctccataga ctttaaaagc gtatcttcag
    14281 taaagtgttt cggtggttga gtaaattttt ctttaatctc tttattttca atgcttaaaa
    14341 catcaccaat gctcacatca ggaagctcta tgaactcatt cttcttggac ttgtattctt
    14401 tgaggtattt gctaaagccc tcgtctctaa ttaccttgcc tgaacttgta aattcaaatc
    14461 cgtcaaattc agctacaatc tttgttgtgt tttccactaa tggatagcct acacttgcgt
    14521 gaagcttatt agaaataagc ctatatacct tcgcttcact atcagggatg ctcgataaat
    14581 cttcacttaa cgagcttact gtcggaataa tagcgtgatg atccgtaact ttttttgagt
    14641 taaatactgt cttgatacgc tctgtatcaa aatcattttt tcctaaaatg ttattgaccg
    14701 tacttacaat catatcttcc gttaagcatc tgctgtctgt tcttggatag gtaattagtt
    14761 tcttttcata taggctttga gcataatcaa gcgtctgctt tgttgaatat ccaaaatatt
    14821 tattacactc tctttgaagt gttgttagat caaagggtaa atccggcttt gtaatctttt
    14881 ctttttgaat gacatcagtt atttcaatat tatcgcctat taaattaata agctgctctg
    14941 cggttatttc atcaccaatt ttgtctgtcg ataatgtaaa gccgttcata gaaagctcta
    15001 cggtgtagta tttttctttc ttaaaattac ttatttcttc atctcttttt acaatcatgt
    15061 aaagtgttgg tgtttgtact ctgccgacag aataattttg cttatacaga caagaataga
    15121 gcctgctgat attcattcca acaagccagt ctgcaatagc tcttgcctgt gccgattcaa
    15181 agagattatc atagtccttt ccgtctttta gattatcaaa tccatctcta atggcactat
    15241 cctccataga tgaaatccaa aggcgtttca tcttcttttt gcaatttgcc tgattatata
    15301 caagtctaaa aatgctctcc ccttctctac ctgcatcgca cgcattgata actgtatcaa
    15361 cctctttgtc gttcatcagc tttttaagga tgttaaattg tttcttggtt gactttgcca
    15421 cttcatactt ataatcctta ggaataatcg gtaaatcagc catattccac ttggcatact
    15481 tttcatcgta agcatccgga tttgccatct gaattaaatg acctacgcac caagatacct
    15541 tatatccatt tccctcatag tatccatctt tcttttttgt agctcctatt acctttgcaa
    15601 ttgatatagc tacactcggt ttttctgcta tcacaagtat atgttccatg tatatttttc
    15661 ctcctgcttt cattgtaaaa gggcgaaaga ttttactctc tcgccctgcg tttgcaccca
    15721 taacaatata ttttaagatg catttattct tcatcttcaa cttctgcttc tcctatttct
    15781 tcctcacttt ctgcttccga aaagaaatca tcatcttctt cctctaatgc ttcaagctcc
    15841 ttatcctctt tcttttttac gaccttaaag taataacctg ctcctaatgc tccgcctata
    15901 acaagaagca gaataatgta tgttcctaaa ttactctttt cttctttttt ctcaggcttt
    15961 gcttcttcct tagttggctc ctctttgcta atctcctgct tcggagcctc tttcttttca
    16021 accatattta gaagatcatc ttcagatact tccgttaaaa gcattacatt ttcttgtgtc
    16081 tcatcgtggt tgatgatgag atggaaggtc ttaccgttct tcgtttgaaa ggtaataaac
    16141 tgtcttgcat cagcagaata cagatctgtt tctttgttat caccgctatc cccatgatga
    16201 attggatagt ccttattcgc attgtcctta ttttccgtaa cggttcctct tgcctttgac
    16261 ggagcagaag ctactccctt attggtattt acagtcttac ttgtcccgtc catagatgaa
    16321 tcctgattgt tattggcagg agctttcggt gtcagcttat taggatagcg aatatctttc
    16381 tctttcgttt cagaattact tgtatctttt acagtatttt ctgaactcgc ctgtgtagtt
    16441 gtgctttttc ctgtattgtt tacaggtgtt tgatttgaaa tgcctgagtt tgtcttaatt
    16501 ccggaaatag ggctgttcag tgcagacgga ttagatatta cgggagcctt gtttgcttca
    16561 tttgcctttt cctcaatctc tttaatcttt ttattcagct tatccatttc cttcttcatt
    16621 tcttctgata agtccttatt ttccttatct tttttcattt tttctttcaa gctttcaatc
    16681 tgggcttcaa gttctttgat tttttctttc tgattatcgc ttaatttgtc tttatccttc
    16741 atttcgctat ttaacttatc aagtttttcc tgaagctcct tagtatcttt ttccatttta
    16801 gaaatatcat ctttagaaag ctctgtctgc gttgatttat cttccgtctt tggagttttc
    16861 gtctgcgagc tttcatcctg tttcggcttt tcgtcttctg tctgagtctc tttatcttta
    16921 gcctcagtct ttgctacctt tcggataatt tcatcatttc catcgccttt tacttcataa
    16981 aatagaaact catcaataaa tttcatatta tcagggagca tcggcagatc accgctgtca
    17041 agaagctgtc ctttctcagc ctttatcttt tcttccttat aaactttttc atcttcaaag
    17101 atatacttta catttacttc tacttgtgtt tggacagctt tatcgcttgt aggtgcttcc
    17161 gttaagcttt gctcatgagc ataaacaact gtccaaagac ccaaaaggca gcttatactt
    17221 acaacgactg caagtgctgc tgtccaaaac ttcttattct ttttccaact ggttttcatt
    17281 tgacttctcc tttttcattt ctactctttg tctgtttacc tttgccatca aatcactgat
    17341 tgtaatgttg ttctttctgc aaatggcaat gatttcttcg ttttcaagtt cttcttcacg
    17401 aataaataaa ggctccaatt cttcatcaat cagagccttt ttatccttca acttctttat
    17461 tttgtttttc actaccgtta attctcgttt cacattgtta cctcctattt ctttacgttt
    17521 ggtgggaatc caaatcctac gggatgatgc ttgcagaaag acgcaatcca attgtctaaa
    17581 gtggttactc ttattcgtcc aatattatcc ataactttac catctccaat ataaattcct
    17641 acatgaccat aagtaagtcc tgctgtgctt ccactactac tgctttccac cgctacaagc
    17701 attcccacct tgagttttga cctgtctgat gtaaaagtat agtttcggta catatcgcag
    17761 gcatttccgc caatatatcc aagccctgca ttttgataga cctgtgatac ccacatggca
    17821 caccagcctg cacccggaga tggcgtgatg tatgcagcat taacaatctt cttttgaact
    17881 tcacttgatg cttcatactc aactccacca cctacgccac catttgcact gataagttca
    17941 gaatttccaa aggcttctcc catatttcct tgtgcaagaa ataaggcttc ataatgcttt
    18001 aggttgtctg gatagtttgc aaagaccttt cggataatgc tgtccatctc ttttttatgc
    18061 agcgttacaa tgagcttttt gtattcataa ggttcttcat gactttcggt atattcgttg
    18121 ccatcttcat cggtataggt ttctgtaact gtcctgtatc tgatttcaac ttcttcccta
    18181 tattcaaggt catacatgga ctcaaacaat tcttttaata tggattctac ttccgataca
    18241 ctctttacct ctccacatct tgatgtaatg taggataaaa gctcatggac attatgacca
    18301 atatactctg tattatttaa gatgtactcg tcatagccgg gatagttatt tttgacatga
    18361 tcaacctcgt tttgaagctc actttccata gcagaaaagt tctgattgat ttcatttagg
    18421 acatttggct ttgacaaata gcttgttgta agaacagaac ttgtggagtt cataaagcct
    18481 gtcattcccg ttcctgcaaa gttgataaag aaagttccca aaataataag acctatgaaa
    18541 ataatcataa gtccctttgc ttttcttact ataaatctct ttgaggcttt caacgagcca
    18601 atgaggtttt ctttaatccg atctctgagt cttgacttat tctcacgacg aatggctgcc
    18661 tttacctgat ttttcttttg aaatctttta taggcatttg ctcttttata ttcattcgtc
    18721 tttttcaggt tctctttggc atcacgaaat tcaagcttcg attttcgctt cctgatttta
    18781 taatccttat ttgtaagatc gtatcctttc ttcgcctttt tcttatcaga atatttcttt
    18841 atgccatgaa tgagtttaga gcttgcgtcc gccgtctttt ctcctgcttc caccccttta
    18901 ttttcatcac ttccatgaga aagataatcc cttacggttt cacttccttt gacaagtcct
    18961 gaaagggcag atacctttac catatctttc tttagctttt tcctctgctc tttagaaagg
    19021 ctgctatgaa ctttttcgct tgcagcatct tttccagtct ttttaccttc agatttattt
    19081 tcctttgaca catcttcttt ttttcttgta taaaggcttt cggtgtagtt tttcctctta
    19141 tactttctct ttttagcctc atgagttata gaggaacttc caaaaaactc atctttttta
    19201 tggagattat catccacatc ataggtcgat tcaaagtagt cgctgtccct aaagtcatta
    19261 tcgtatctgt ctataatccc gtcattatca aggtcttttc ctaaaggatc atagattttt
    19321 ccatccttta cctccgtaat ataatccgat cttcctgttt cacttacagc agctacacta
    19381 tccttatcag aagctctata tcttgcgttt ctcttggatg ttccataagc cttttcatta
    19441 tcagaactta ccttgattgt tttcttatga accttatcct gaaatctgtc tttgtcatgg
    19501 gcgatttttc ctctgtaatc atcgttatgc tttagcttgc tttcttcaga tgtagtgaat
    19561 gtttcactat ggagcatttc tctttcaaga cttgccttat gcctttccct aaaatccttt
    19621 ttcagctttt ttcccatagg ctacctcact tcttcaggct tggtagtcat cttctgatag
    19681 agaattgtat ctttcgggaa cttatcaagg aaaggaacaa tggtatttcc aaagaacagt
    19741 agtccctcgc cctcatttga gttggtaaca tatctaagct gaggaagtga aattttaagt
    19801 tttcttgcta aaatttctct atctcctgac gcttgattaa gcattaagac aaagtccgta
    19861 ttgtcaaaga tattttcaat ttccttactc ataagtaggt ctttgacatt ttgcgtaata
    19921 cctgttggaa tacctcccca ttttctgaaa cgcttccata tttccacaga ataagatgct
    19981 gtctgctcat cttttagcag caagtggaac tcgtcgatat aatatcttgt cgctttgttt
    20041 cctctgtttt gcgatacctt attccacacc tgatcctgta taacaagcat accgattttc
    20101 tttagctgac ttccaagttc tttaatatca aaacaaagga gcttcttatt taagtccaca
    20161 tttgacctgt gattaaaaac attaagggaa cctgatacat agatttccat ctccgttgcc
    20221 agctttttac cgaccttttc ttcctgtcct tttaacatat cgtataggtc ttgaagtata
    20281 ggcatattac aaggttctgg attttcaaaa tacctttcgt aaatcttagg taagcacctg
    20341 tctataacgg acttttcttc tgccgtaaga ccactaccgc ctactacaag ctccagcata
    20401 ctcataatga agtttgcttt gtctttcagt ggcgcatccc catcgccata gttcatattt
    20461 atatcaaggg gattcaggaa atctttggac ttactgctga ctttaatgac ttctccatta
    20521 aattgtctta caaggtttcc atactctcct tccggatcgc agataatcac atcatcgtct
    20581 gttactaaaa ttgcatttgc catttctctt ttagcactaa aggatttacc acttccagga
    20641 gttccgagga ttagaccgtt tggatttttg agttttttcc tatctgccat aatcaggtta
    20701 tggcttaagg catttagtcc gtaataaaga ctgttacttg aattgataaa aagctcctct
    20761 gtggtaaagg gcataaaaac tgccgtagat gatgaggtta gtcctctatc tatctcaatc
    20821 ttatttacgc caagaggtag cacgctgata agtccttgtt cttgcgcatg gtcaagtctt
    20881 ttcagcttac agttatgctt atttgcaatg gaacttatct gagagattgt gttatcaagc
    20941 ttttgcaccg tttttgcaaa gttcataaag acgatactta ccacaaacat tcgttcatct
    21001 cttgtctgca agtcttttaa gagactcttt acatcttctc cataggtgat taagtccgat
    21061 ggaagaatgt ccatatcata accgcttctt accgctttct tattttcctc aatcttcatc
    21121 ttgtcaatat cggtattttt tctctttacc attttaatgg cttccgattg gtcgattgcc
    21181 ttaatatgaa atgaaatatt gatgttatca tcaatatcca aaaactcggc aagcatacgg
    21241 tctgaaagct cacttgcaag aatttgaaaa tgacttactg ccccaataaa ttttccaaac
    21301 ttaaaatacc ttgacggagt aaagttaaat tcatcaggag caatatatgt ctttgtgctt
    21361 tcttttttct ttaagtcctt gtatgaaaat tcaaaggtct tattgggatt taatatatcg
    21421 tgaagtatct ttaatctttc ttctcctgta agactttcag cacgaacccc catacttttg
    21481 agattagata atatatctat ctccagtctt tcaagttttg atgttgcctg ctctaaatta
    21541 tccgcttcta ctgtaaaggt tacatacttt gattttttca gtccgttatt tccctttaca
    21601 atctggcttt taagcatctc tctaaactca agacgtatat cgtcaaaacc gtccttttta
    21661 tccggtatct gaattgccga cttcatttct tcgtttctac cgagttgatt gatgtaggaa
    21721 aactcaatac tgatgctcgg atcaaatgag tttagaaaat ttgcaaactg attaaaaatc
    21781 aagtctcgat cttcatctaa agcaagttgg tagttaatat cctgaaaggc tatgctttta
    21841 ctaaagtgct tttcgtcaag ctgacatata ccgcttttta agagtctaag atagggaatg
    21901 gtatcttcga cagtatatct ttttggttct tttctaaaaa taaggtcaag tagcccacct
    21961 ttatcttttt tagattttcc cttattttgc tttaagtcct gtttttgact tcttaacttc
    22021 tttttgtttt gttctaactt tacctgctga atctttcttt tgtctttcaa ggtaaacctc
    22081 ctttctcact cttttttgtg gctgataaaa cttatgaagg taaatatatt taaaatattt
    22141 ctcaaaggtc agtccgtcct tttcaaaaag agtgatgaaa aatataggga gtgtggacac
    22201 aatgagaaat atgacggcta tatcatttgg aacgaccttt cgcataaata aatagactgg
    22261 tagtcctacc agtcctgcga gtgtgaaacc tatcatctgc cttttggtta agttaaaagc
    22321 tacctttgtt tttaccttct ttaggtcttt tgggattggt acatacgcca taacttacct
    22381 cccatcttct ttttcctttg aaagctctgc catgtgttca taaacagagc cgatttcttc
    22441 ctgaatgctt tctatctgtt tatctatatt tttgttatat ctttcctgca aaatacaaaa
    22501 gtcattatgg cagcgaccga tgtcttttgt tctttcttcc aagtcattaa tcttgctaaa
    22561 aagtttgatt ctatcaatca ccgctataac acctgctccg attactactg ctgtaaatac
    22621 tgtttttctt ttgatcatcc tatccttcct ttctgttagt gtgcatttaa tacgcttttg
    22681 gccagagttc cgctctttag catcattagt cccagcaaaa ccgcatatcc aagtatcgtc
    22741 atggtacttg tgtgtatatc tgttatctgt attgtcttaa ctaatactgc gtatattcca
    22801 agacaaacca ttaaaaagag tccttgtagc ccaagtgcaa atagtccctt gatgtaattt
    22861 gttccaattt gtccccactc tttgtttccc attgtggcaa atggaatggc tgaaaccgat
    22921 gagtaaacat aaatctcaaa cattcttccg taaaccacaa gcatgattac aatagaaatt
    22981 acctctatgg caacctttat gagtgaggtt tcaaagagaa tcatgacaag ctctccaagc
    23041 cccttttctt ttaaggtatc catcattgcc actatctgat ctccggaaac ggtggcagag
    23101 gtatttatca cccctgccgc cttatttacc atatgttgtg ccacatcaaa aactgccatt
    23161 gaaaactcaa aagcatgaga tactaaccat acagcaatcc acatcttgat gatgtactta
    23221 aaaaattcaa aggtatctgt atcatgcata ttgttctttt gcattaccat attgataagc
    23281 tcaatacaaa ggactgctgt gatgattagt cccgctatgg gaataatgac ggaatcgtta
    23341 atgcttttga taaaggcaaa tacatctccg ttccacccca tgggagtttt tcctacatct
    23401 gttgcaactg caccaacttt atcgttgatg tcaagaaaca tggactctaa gtttgcttgg
    23461 ataccgccca gtagaagttc cttaaaaaat tcttctatct tgtcgaatat tccaaacatc
    23521 taagctctcc ttttcttctt acttgagtac attggcaaga agtggaatca gcttaagtcc
    23581 gattaggaca ataccgcctc ctgccatcag ctgcttaata ccttgagatt ttgcacccgg
    23641 attgtcattg ccatatcctt ccatcaggtt gataacaccc catgctccaa gtcctgcacc
    23701 tactgccatt accaaaatct ttaatacatt aactgcctgt gtaaaaaatt ccataattta
    23761 ttcctcctcg ctttcttctt tcttttcaat cttgttatat gacttcacta caaagttttt
    23821 gtaagtcttt ccctcttttt cacgtttctt aaagtaacca aagatatgaa tcaaatcgcc
    23881 tttttcaaag tcctttgctt tctccgattt ttctccatag gctgcacagt tgatatactc
    23941 cttgcctttc ccgtactttt ttacaagcgt gaagtttgca acctcaacag tttctccctc
    24001 tttgtcaaaa cttgaaaaag tgggctctgc caataaattg gcattgatgt taatcatttc
    24061 ttgcttcatt aaaaatttct ccttttcttt caataaaaaa gcgatcggag ttttttcttt
    24121 tccaatcgct gatggtcatc ctatttactt ttttcttagt gggacttcgt attcttatca
    24181 tcttacctcc tcgtatatta tagttgtagt aataaatact acattttgtt ctcaatccca
    24241 ctttcttatg ggataataat tgtatagata gaggtcgtag ttaacctcct tgaagctagt
    24301 cttcctaaat tagggttacg cctcttcttt acgtctttat cagtatgagc tggatagcgc
    24361 atttcgttgc tgtatgtcta acgaggtttg attgtcgtaa agttcttgag ggtcctttct
    24421 ctatctaaat ttatattaaa gctaggtgat tttatgtttt acattggtgt tgatattgca
    24481 aagaaaaacc acgaagcctc aataattgat tctgatggga aacttgtttc tgagtccttt
    24541 tctttttcta attctattag gggtttagag aagtttcaga aatttatttc ctctttttcg
    24601 attgattcta acaactgtat tattggtatg gaagctactg gccattattg gctttcgctt
    24661 tattctttct taattgattt aggtttttct tgtgttgtta ttaaccctat tcaatctgat
    24721 gcttttagaa agatgtatat tagacagact aagaatgatt ctgttgattc ttttgttatt
    24781 gctcaaattc ttaggttcgg tgagttttct gtttctcatt tttctgatga ggatactttt
    24841 gctttaagaa atctttctag gttcaggttt gctcttgttg atgaagcttc tgattggaag
    24901 agaaagcttg tttgtatttt agatcaggtt ttccctgagt attcttcgct tttttctaat
    24961 atttatggtg tggcttctaa ggagcttttg agtaagtatc ctttgcctga ggatatgatt
    25021 tctattcctg ctgatgagct tgctaagatg ttgtctaagt gtagtaaggg tcgctttggt
    25081 attgataagg ctaaagaaat tcaagagaag gcttctaatt cttttggtgt taagtttgct
    25141 ttaaagtctt tttcttttca aattaaacag attattgctc aaatttcttt tcttgaagat
    25201 cagatagctg atattgaaga tgaaatttct tctatgatta attctttatg ccctgttatt
    25261 acttctatca ccggtattgg tgatgtctta ggtgctgcta ttttctcaga aattggtgat
    25321 atttctcgtt ttgagagagc taatcagttg gttgcttatg ctggtttaga tgttgctgtt
    25381 aagcaatctg gcaattttaa tgctactgat actaagattt ctaaacgtgg ttctccttat
    25441 cttagacgtg ctatttggct tgctgctagt gttgctgctt ttaaagatcc tgctttgtct
    25501 gtttattatc aaaagcttag acaaaggggt aaggcgcatg gtacagctat tggtgcagtt
    25561 gctagaaaac ttactaacat aatttttgct gttttaagag ataacaaaac ttacataccg
    25621 aatatttagt tgttatttag tttttctaag cctaattttt aggcttattt gatgtgccat
    25681 aaattacaag attaattatt ttttattttc tc
//
LOCUS       DATRSD010000020        25581 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRSD010000020 DATRSD010000000
VERSION     DATRSD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25581
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.020"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(8..394)
                     /gene="rplQ"
                     /locus_tag="VBD85_000655"
     CDS             complement(8..394)
                     /gene="rplQ"
                     /locus_tag="VBD85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO7133222.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(409..1347)
                     /locus_tag="VBD85_000656"
     CDS             complement(409..1347)
                     /locus_tag="VBD85_000656"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO7133223.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1397..1780)
                     /gene="rpsK"
                     /locus_tag="VBD85_000657"
     CDS             complement(1397..1780)
                     /gene="rpsK"
                     /locus_tag="VBD85_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO7133224.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(1798..2163)
                     /gene="rpsM"
                     /locus_tag="VBD85_000658"
     CDS             complement(1798..2163)
                     /gene="rpsM"
                     /locus_tag="VBD85_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO7133225.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2181..2297)
                     /gene="rpmJ"
                     /locus_tag="VBD85_000659"
     CDS             complement(2181..2297)
                     /gene="rpmJ"
                     /locus_tag="VBD85_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO7133226.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2323..2541)
                     /gene="infA"
                     /locus_tag="VBD85_000660"
     CDS             complement(2323..2541)
                     /gene="infA"
                     /locus_tag="VBD85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO7133227.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2657..3295)
                     /locus_tag="VBD85_000661"
     CDS             complement(2657..3295)
                     /locus_tag="VBD85_000661"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO7133228.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGESILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3390..4694)
                     /gene="secY"
                     /locus_tag="VBD85_000662"
     CDS             complement(3390..4694)
                     /gene="secY"
                     /locus_tag="VBD85_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO7133229.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(4715..5155)
                     /gene="rplO"
                     /locus_tag="VBD85_000663"
     CDS             complement(4715..5155)
                     /gene="rplO"
                     /locus_tag="VBD85_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO7133230.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5280..5459)
                     /gene="rpmD"
                     /locus_tag="VBD85_000664"
     CDS             complement(5280..5459)
                     /gene="rpmD"
                     /locus_tag="VBD85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO7133231.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5474..5968)
                     /gene="rpsE"
                     /locus_tag="VBD85_000665"
     CDS             complement(5474..5968)
                     /gene="rpsE"
                     /locus_tag="VBD85_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO7133232.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(5987..6343)
                     /gene="rplR"
                     /locus_tag="VBD85_000666"
     CDS             complement(5987..6343)
                     /gene="rplR"
                     /locus_tag="VBD85_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO7133233.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6444..6980)
                     /gene="rplF"
                     /locus_tag="VBD85_000667"
     CDS             complement(6444..6980)
                     /gene="rplF"
                     /locus_tag="VBD85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO7133234.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7090..7488)
                     /gene="rpsH"
                     /locus_tag="VBD85_000668"
     CDS             complement(7090..7488)
                     /gene="rpsH"
                     /locus_tag="VBD85_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO7133235.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7643..7828)
                     /locus_tag="VBD85_000669"
     CDS             complement(7643..7828)
                     /locus_tag="VBD85_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO7133236.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(7846..8388)
                     /gene="rplE"
                     /locus_tag="VBD85_000670"
     CDS             complement(7846..8388)
                     /gene="rplE"
                     /locus_tag="VBD85_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO7133237.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8412..8717)
                     /gene="rplX"
                     /locus_tag="VBD85_000671"
     CDS             complement(8412..8717)
                     /gene="rplX"
                     /locus_tag="VBD85_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO7133238.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(8797..9165)
                     /gene="rplN"
                     /locus_tag="VBD85_000672"
     CDS             complement(8797..9165)
                     /gene="rplN"
                     /locus_tag="VBD85_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO7133239.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9190..9450)
                     /gene="rpsQ"
                     /locus_tag="VBD85_000673"
     CDS             complement(9190..9450)
                     /gene="rpsQ"
                     /locus_tag="VBD85_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO7133240.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9476..9682)
                     /locus_tag="VBD85_000674"
     CDS             complement(9476..9682)
                     /locus_tag="VBD85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO7133241.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9692..10105)
                     /gene="rplP"
                     /locus_tag="VBD85_000675"
     CDS             complement(9692..10105)
                     /gene="rplP"
                     /locus_tag="VBD85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO7133242.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10109..10762)
                     /gene="rpsC"
                     /locus_tag="VBD85_000676"
     CDS             complement(10109..10762)
                     /gene="rpsC"
                     /locus_tag="VBD85_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO7133243.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(10775..11119)
                     /gene="rplV"
                     /locus_tag="VBD85_000677"
     CDS             complement(10775..11119)
                     /gene="rplV"
                     /locus_tag="VBD85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO7133244.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11135..11413)
                     /gene="rpsS"
                     /locus_tag="VBD85_000678"
     CDS             complement(11135..11413)
                     /gene="rpsS"
                     /locus_tag="VBD85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO7133245.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11512..12345)
                     /gene="rplB"
                     /locus_tag="VBD85_000679"
     CDS             complement(11512..12345)
                     /gene="rplB"
                     /locus_tag="VBD85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO7133246.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12363..12659)
                     /locus_tag="VBD85_000680"
     CDS             complement(12363..12659)
                     /locus_tag="VBD85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO7133247.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12659..13282)
                     /gene="rplD"
                     /locus_tag="VBD85_000681"
     CDS             complement(12659..13282)
                     /gene="rplD"
                     /locus_tag="VBD85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO7133248.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13306..13932)
                     /gene="rplC"
                     /locus_tag="VBD85_000682"
     CDS             complement(13306..13932)
                     /gene="rplC"
                     /locus_tag="VBD85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO7133249.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14037..14345)
                     /gene="rpsJ"
                     /locus_tag="VBD85_000683"
     CDS             complement(14037..14345)
                     /gene="rpsJ"
                     /locus_tag="VBD85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO7133250.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14550..15830)
                     /locus_tag="VBD85_000684"
     CDS             complement(14550..15830)
                     /locus_tag="VBD85_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7133251.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITVYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTLLIGAILGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGMLTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIL
                     FELGLLGVWMATIADNIFRWLFLKVHYHRYIQKM"
     gene            complement(15935..17425)
                     /locus_tag="VBD85_000685"
     CDS             complement(15935..17425)
                     /locus_tag="VBD85_000685"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO7133252.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPLVKVGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDNKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKNGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSILRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQPINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPRAVTDLQKAEVIHRTVVPTSNMRETLEECLGLKQ"
     gene            complement(17545..18561)
                     /gene="adhP"
                     /locus_tag="VBD85_000686"
     CDS             complement(17545..18561)
                     /gene="adhP"
                     /locus_tag="VBD85_000686"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO7133253.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKIAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(18740..21382)
                     /gene="adhE"
                     /locus_tag="VBD85_000687"
                     /gene_synonym="adhC"
     CDS             complement(18740..21382)
                     /gene="adhE"
                     /locus_tag="VBD85_000687"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO7133254.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21652..23430)
                     /locus_tag="VBD85_000688"
     CDS             complement(21652..23430)
                     /locus_tag="VBD85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7133255.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLLTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLISMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYVIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23427..23807)
                     /locus_tag="VBD85_000689"
     CDS             complement(23427..23807)
                     /locus_tag="VBD85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133256.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(23814..24251)
                     /locus_tag="VBD85_000690"
     CDS             complement(23814..24251)
                     /locus_tag="VBD85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO7133257.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVNDGCKQWLTLINNSRD"
     gene            complement(24403..25401)
                     /gene="ruvB"
                     /locus_tag="VBD85_000691"
     CDS             complement(24403..25401)
                     /gene="ruvB"
                     /locus_tag="VBD85_000691"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO7133258.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
BASE COUNT         7675 a         5312 c         4386 g         8208 t
ORIGIN      
        1 ataaaaatta tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg
       61 ttttaagaat acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa
      121 aaagtttttg aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg
      181 atgcaatttc attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt
      241 tacctagagt aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag
      301 ttacaattga ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt
      361 gtgagctagt gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt
      421 ttttaatcca agacctagat cggcaagttt gattttaact tcttcaagac tcttacgtcc
      481 taagtttcgg actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt
      541 aattccagca cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt
      601 gcggtcaagc actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt
      661 agcaacctct gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag
      721 accaagagca tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc
      781 aaagccatcg ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg
      841 tgtatagatt gaatctacag ccaatgttcc cactggtgca tcatcttttt tatttccttc
      901 tgctggaaca taacctcggt ttttagcaac tgtcattgtt gctttcaaac tgtgaccttc
      961 agcaattgtg aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat
     1021 atctccggca gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc
     1081 gacgtaagat ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg
     1141 tacacctggg atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc
     1201 tgcacctgga agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta
     1261 gccacgttct agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt
     1321 tattattggt ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta
     1381 ctataaaatg ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta
     1441 caggagtcac atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag
     1501 cagattcacg acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt
     1561 cttgtgcaga tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac
     1621 gagaaccttt aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat
     1681 ctgtaatcat aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga
     1741 tgttcttttt cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta
     1801 ttttttctta cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt
     1861 attttgtcca cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc
     1921 catcaaacgt ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc
     1981 atcaacttca cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc
     2041 ttcagaaata cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac
     2101 ataagtaagt gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc
     2161 cattttatct cctttctata ttatccttga cgttgtttgt gttttgggtt tgtcggacaa
     2221 attaccataa cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc
     2281 gatggtctta ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt
     2341 gatgcgtcca cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa
     2401 aatacggatg tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt
     2461 ttccaactca acagtaaaca ttgcattagg catagtttca acaactttac cttcaatttc
     2521 aatcacatct tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctgt
     2581 aaagaggtaa caattataag tcagactaac ttagtatatc attactaatc tacttatgca
     2641 agtagattag cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt
     2701 ctgttatttc ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa
     2761 gaatagattc tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat
     2821 catcttcacg ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt
     2881 ggaaagtttc accagtttta cgattgataa tacgaccact caaacgctct ataagacatg
     2941 atggatccac tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag
     3001 catctaaggc gtgtgcttgt tcaatagtac gtggatatcc atcaagtaaa aaaccttttt
     3061 ctgcgatatc atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa
     3121 ccaattcacc tttatcaata taacttttag ctaaacgtcc catttcggtt tgattagcca
     3181 ttgcggcgcg gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacgatct
     3241 tagctgcttg agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag
     3301 tctccttatt ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa
     3361 atagaaggtt gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag
     3421 cgtctcttca aaagatagcc ctcaagttgt ttcatacctt caatacctgt tgcaatcaaa
     3481 atgagtaagc ttgtaccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca
     3541 ataattggta acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc
     3601 aatagcgatg acatatactc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac
     3661 gaattttttt gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag
     3721 aaagagaaca gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta
     3781 agtaattctt ggagtttagt aagccaagga atttctttac cattttgaaa gaaaggaata
     3841 atcgtacttg gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga
     3901 tttactttta gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg
     3961 attggaattt tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact
     4021 gctacaataa gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt
     4081 acaaagaaat cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatccccgca
     4141 aagataatca tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa
     4201 gtaaccacca tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc
     4261 ggagttttaa ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat
     4321 tgtacgaatg caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct
     4381 acctctcctt gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga
     4441 acaacaatag acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc
     4501 attgcattac cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct
     4561 aaactcttaa cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg
     4621 ataaagatag taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt
     4681 aattttaaaa acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt
     4741 tagcagtgat agctgcttca gcagatttag aaaatttagc tgctttaaca gacaatttct
     4801 tggttaattc accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag
     4861 cttctttaag aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa
     4921 ggttcacaag agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacgac
     4981 ggaacaatgg agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt
     5041 tttgtccttt ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca
     5101 cacgattgcg gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt
     5161 tttctcctta tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga
     5221 ctcgcctata cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcatttaat
     5281 taagcttctt caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca
     5341 ttatcttcct taacaactga agagtttaat ttaccaagtc caagggcaac aactgtttta
     5401 cgttgttctg gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt
     5461 ataatcccct ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt
     5521 ctgcacgttt taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt
     5581 ttgaaccaag tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa
     5641 cagcaccacc agcggcaacc ccagcaccct caacagctgg tttcaacaat actttcgctc
     5701 caccaaattc tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca
     5761 tgtttttctt agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac
     5821 ctgtaccaaa accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta
     5881 gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa
     5941 gttctactgc attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc
     6001 cgttttcacg agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc
     6061 ggtcaaacac cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga
     6121 caacaacggc ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac
     6181 ttgcgagcgt tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac
     6241 ggaaaatgtt caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt
     6301 ggcgtttttg gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa
     6361 tatattgtta tgtcaaaatg acaatattaa aatgaaagga tagagaattt gcagaaaaga
     6421 ttctacaaaa tctatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat
     6481 tcaccaacgt aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg
     6541 taagcagcag tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga
     6601 gttggcactt caaaagtaac accttctgga gcttcaactt cgtcttggtg agacttacca
     6661 acagaaagaa caagttttga tccttgaagt tgtgcacgat aaccaacacc gcgcatttca
     6721 agagcttttt tgaaaccttc agaaacacca acaaccatgt tgttcaagtt cgcacgagtt
     6781 gtaccatgaa ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc
     6841 ccttcaacag tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct
     6901 tttacagtaa caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact
     6961 ttattaccaa tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa
     7021 ctagttttca cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta
     7081 ggtaaaattt taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc
     7141 tttgtcagtc aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac
     7201 tttaggcata tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa
     7261 gttagtgata acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg
     7321 tttgtcatct tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc
     7381 aatccctttt ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc
     7441 attacgaata cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc
     7501 ctcttattag cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttgta
     7561 agactaggtc ttagtatatt tgcgtttaaa aatcagtgcc attatcaacg acacctttta
     7621 aacacaaagc atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt
     7681 tataagccaa gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg
     7741 gacgtccgca tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt
     7801 tcttagcaat catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg
     7861 cattccaagg ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat
     7921 aacgatatca agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa
     7981 ttgttcttta actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc
     8041 atggaagtca cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat
     8101 acgttcacca cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc
     8161 agcgattgat ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc
     8221 agcagcagct ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag
     8281 aacgattttc tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa
     8341 cgcaggaaca acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct
     8401 ttccttcgtg attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg
     8461 tcaacaactt tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt
     8521 gacacatgga ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt
     8581 ttttgatgtt tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga
     8641 gctttgagaa ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg
     8701 ccttttttta caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct
     8761 aggaaatcct aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga
     8821 aacgatcttc atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt
     8881 tccgcgagga gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta
     8941 tgaaccgtct gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc
     9001 acctttttta actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc
     9061 gatgttagcg aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc
     9121 accgctatta tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt
     9181 ttagtttgat tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt
     9241 agctgaaagt gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt
     9301 ttcatcatgt gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt
     9361 acgtttagtt tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc
     9421 gtaaagagtt ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt
     9481 tcatttctga ttgcacagtt ttaacacgtg caatttgttt cttaacttcg tcaaggcgag
     9541 cagtcttttc aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga
     9601 gttcgttttc tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat
     9661 ctttaatttc ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc
     9721 atttaactgg taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa
     9781 caccagcaat ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg
     9841 cccctttacc agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga
     9901 agattttaat ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag
     9961 cttcgatttg acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga
    10021 atgaaacttc tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt
    10081 gtttaacacg tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg
    10141 gaagaacttc tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag
    10201 cttcttccca agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg
    10261 aataaccttc agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa
    10321 tacctttagc tcctgcacgc attgtacgtt gaattgcttg tttttgagca cgacggaaag
    10381 caacacgttg ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag
    10441 gttgtttgat ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac
    10501 cacgaagagc gtcaacgttt gctccacctt taccgataac catacctggt ttagcagtgt
    10561 gaagtgaaac aataactttg tttactgcac gttcaatttc aatagttgaa actgatgcgt
    10621 cagctaattc tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt
    10681 attctttttc agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa
    10741 ttggatgtac tttttgaccc acgattttac ctccttattt ttctgataca actacagtta
    10801 cgtgagttgt acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt
    10861 tcattgttgg tccttcgtta gcgaatgttt cagatactac caagtttgct ttttccaaac
    10921 caaagttgtt ttctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg
    10981 ctttgtttgg agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa
    11041 gatcaagtac taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag
    11101 ctgaagtaat ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag
    11161 ctgcgtgacc tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt
    11221 cctcttggat ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga
    11281 tgaaacttgg gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt
    11341 catttgcttg agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa
    11401 gactacgtcc cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact
    11461 atagtgtact accgagctgg cggatttgta taagattatg ctattaacct attatttttg
    11521 gttacgacga cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc
    11581 aagtgcaggt ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc
    11641 accaccaccg tgtgggtgat cattagggtt cattacagaa ccacgaactg tagggcgaac
    11701 acctttccaa cgattacgtc ctgctttacc aatgttaaca agtgattgtt gttcattacc
    11761 aacagtacca attgttgcac ggcaagtacc aaggatcata cgtacttcac ctgattgaag
    11821 gcgaacaaga acatatttac cttcttgacc aagtacttga gcagaagcac cagcagcacg
    11881 aatcaattca gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg
    11941 aatgttagca agtggaagtg cgttaccaac tttgatatct gcttctggac cagaaataat
    12001 gcgttgacct acttcaaggc ctttaggcgc aaggatgtaa gctttaacac catcagtata
    12061 gtgtacaaga gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc
    12121 ttcaacacca tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc
    12181 accttggtga cgaacagtaa tacgtccgtt attgttacgt cctgctttat tctttagtga
    12241 aacaagcaat gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat
    12301 attacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta
    12361 gattattcag cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgatg
    12421 atagcttttt tagtttttga agtgaaacct gtgtaacgac caacacgttt tgcttttggt
    12481 ttaactgtca cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct
    12541 tgtttgatca aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca
    12601 agagcaacca ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt
    12661 atgcaagaac cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt
    12721 taacgatatc aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac
    12781 gcgctgaaag tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa
    12841 tgctaagtgc tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt
    12901 tttctacagc tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa
    12961 ggcgacgaac tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa
    13021 caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt
    13081 tttgacgcca tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag
    13141 taccttggcg aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg
    13201 gttcgatacc gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt
    13261 caaatagttt aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt
    13321 aactgctgat ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag
    13381 gacaacgttt ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt
    13441 accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc
    13501 catagaacct ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc
    13561 atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac
    13621 atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt
    13681 tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc
    13741 aggtttgtta ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc
    13801 gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac
    13861 agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat
    13921 tccttttgtc atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttatgag
    13981 cgttttttgt gctcaaaaaa gttttttacg agcgtttttt gtgctcagaa atcaaattaa
    14041 agtttgattt ctacgttgac accacttgga agatcaagtt tcatcaaagc atcaacagtt
    14101 ttttgtgttg ggttgatgat gtctaccaaa cgtttgtgtg tacgcatttc aaactgttcg
    14161 cgagaatctt tatatttgtg agtcgcacga ataattgtgt aaagactgcg ttcagttgga
    14221 agtggaactg gtccagcaac tgtagcacct gtacgtgttg cagtttcaac gattttttct
    14281 gccgctgtat caagtgtacg gtgttcgtac gctttcaaac ggatacggat ttttttgttt
    14341 gccatctttt ctcctctcgt ctatttacga taataggcta gctcctcaag aaaaccaaca
    14401 cgagttgcgt ggcaatgcaa cctagcgtgt cgcaacctct tgcatcatag ctaaagctgt
    14461 tcatacacag caccagtata tgatatcaaa aaaaaacagc atacgcaagc attattttat
    14521 ttttttcaaa aaaatgcaac catttctggt tacatttttt gaatatatcg atgatagtga
    14581 actttcaaaa acagccacct aaagatatta tctgctatcg tagccatcca aaccccaagc
    14641 aaacctaatt caaaaagtat tcctaataaa taaccaagaa ctactctgat taaccacatg
    14701 cctatagtcg ttgtgtaaaa tgggagttta gcatttccta atccttgcca agctgctgta
    14761 taaaccaagg tcccaatagt agcaggtact cctacgaaag atagcaaaat aacaatcaaa
    14821 cttcctttaa tggcagaagg attatttgta aataactgtg tcaatggttt gccggcaaga
    14881 agcataaaac tcgatataag gatcattaga actaagccaa tataatacga actttgaata
    14941 gtttcctcta tagattctct atttttttgt cctacatatt ttgcagttag gataattgta
    15001 gcagttgcga tgccaagtcc tggcatataa ttaaattgtg tcaaggtttc tccaatagca
    15061 tttcccgcta caacatttgt acctaactga acaacaatag caactataac aacatctcca
    15121 gctcgcatca tcagacgttc tcctgctgat ggtaaggcaa gattccaaat ttgtgcactg
    15181 attttccaag tcattctttt tattatcttt ttaataggaa gatacttcgc aaggatacaa
    15241 ataccaatta atcttgcgat aagagttgat actgccactc cgaccaaacc atagcgccac
    15301 tcaaaaatag caaaacctga taagactgca ttgaggaaat tgacaaaaat actgacatac
    15361 attggtaaac gaggttgccc ttgcacacgt aaaaatgagc ctagggtggt taacattcct
    15421 aaagtaacaa cacctccacc cacaattgct aaatataaac caccaacttg tgcgacactt
    15481 tttgttgtcc ctaaaagttt aaagaaagtt tgtccaaaaa caatcgagat aattcctaat
    15541 attgccccta tcagtaatgt tagaaaaatg gcttgagaac atactgaaat tgcatcatcc
    15601 ttctcactac ctgctaacga cttggccaat agacttgcta tactcgcccc taaagctata
    15661 aagatagctt gataaacagt aattatatta ttagcaactg aaacacctga tactgctaca
    15721 acacctaact gagccactag ataattatca actactccca tgagcatttg taagatattt
    15781 tcaatcatag ctggtatagc taactgaata atctctttac tataacgcac agtatatcct
    15841 ttctattacc cagaatcatt aaaaagagag tgatgggaaa atttctaaaa tgtttctttc
    15901 tcactctaaa tattttaaaa caattgtatt tttattattg tttcaaacct agacactctt
    15961 caagcgtttc tcgcatattt gaggtaggta ctaccgttct atggattact tcggcctttt
    16021 gtagatctgt aaccgcccta ggtaaagaaa cacctgacaa atcgtttaag attgaaatag
    16081 cagcgaaatc ctgtatctct tctttatttg taatagcctt tgtaacaaca cacgggaact
    16141 tataaggact tgctgttgac acaattactg ttggagtttg atctttagtt tctgtctgat
    16201 aagcttggta aacagctgat gctacagctg tatgcggatc ctctatatac tgatactggt
    16261 caaataaacg tttgatgtct aattcaacaa attgttctgt cgcaaatccc gctacaaagc
    16321 tctctaaaat atttgcttgc ctagcctcta aggcatactc cccagtagtc actaaatctt
    16381 ccattaattt tttagtcgtt tctgcatcat cgcctaaaag gtgaaagatt aatcgttcta
    16441 gattagagga gaccaaaata tccatagagg gacttgatgt cactttaaat tctcgatttt
    16501 tatcgtaggt ttgtgtctta aaaaaatctg ttagaacgtt attatcattt gaggcacaaa
    16561 tgagtttggt aacaggtaat ccaatttgac tagcataata tgctgctaaa atattaccaa
    16621 aatttcctgt aggtacacta aaattaattg gttgaccaat tgagatttcc tttgacttaa
    16681 ctaattgagc atatgcatat atatagtaaa caatttgagg aacaaggcga ccgatgttca
    16741 ttgaattggc tgatgataac tgcatatgct gctgtgataa ttttgatcga agaatggaat
    16801 tattgaacat ttctttaacc gaagtctgtg catcatcaaa atttccttca atcgcaacaa
    16861 catgggtatt ttgcccggct tgtgttatca tctgcaattc ttggatataa ctaacaccat
    16921 ttttaggata aaagacaata atctcagttc ctggaacatc tgcaaaacct gccatcgctg
    16981 ccttgccagt gtcacctgat gttgctgtta aaataacgat tttattatcg accccttgtt
    17041 ttttggctgc tgttgttagc aaataaggaa gaatggataa agccatatct ttaaatgcaa
    17101 ttgtaggacc gtgaaataat tcaaggtgat accggtctcc aacctttact aacggggcta
    17161 tttcagttgt atcaaattta gtatcataag cttgactaat acagtaatcc aattcttgtt
    17221 cagtaaaatc atcaaaaaaa gctgacaaaa cgagtttagc cacttcttgg taggaagcat
    17281 ccttaagttt agtgaaatct aaatcaactt ttggaaatgt gatgggggta aagagtcctc
    17341 catcgtcagc taatcccttt agaatcgctt gactagctgt cactttatta ctagcatcac
    17401 gtgttgattg atatactatt gccataagaa tccctcttta tataaatatc ttttagttat
    17461 tgtaacacaa tttaataaca tgtccattgt tcgttatata atcaaccccc tttattaaaa
    17521 taaagggggt tcgtaacatc attatcactt tgtaaagtca agaactttac gcccttgaat
    17581 caatcctctt tccatttcat cgaatacttg aggagctgta tccacaggca ctttttcaac
    17641 caccggtact accaagcctt ctgctccaaa tgcgaaggct tcttctaaat ctttacgagt
    17701 tccaactaag gaaccaacca cacgaatccc atcaagtact gtttttacaa tactcaattc
    17761 catatattct gatggaagac caacagctac cactgtacct ccagcacgaa cgctgtcaat
    17821 cgcctgatta aacgcaattt tagaaacagc agtcacaacc actccatgac aaccgcctgt
    17881 cttttcttga atataggccg caacatcttt tatttctttg ccattaactg tcaaatctgc
    17941 tccaacctct ttagctaatt gaagtttatc tgcgttaata tcaacagcta caacatgagc
    18001 attgaatact ttttttgcat attggactgc taagtttcca agaccacctg caccatacac
    18061 tgcaatccac tgaccaggag cagctccagc ttctttgata gccttgtatg ttgttactcc
    18121 agcacaagtg attgatgatg cttgagctgg atctaatccc tcaggaacct taaccgcata
    18181 gtccgcggta acaatagcgt attcactcat accaccatca acactgtatc cagcattttt
    18241 aacactacga caaagcgtct cacgtcctgt agtacagtat tcgcaatgac cgcatccttc
    18301 aaagaaccat gcaatagaga cacgatcacc aaccctcaaa gacgttacgc cttctccaat
    18361 ttcttctact ataccaatgc cttcgtgacc caaaatgcgt cctggtacct ggccgaaatc
    18421 tccatgtgca acgtgtaaat cagtatggca cacaccacag tattctactt ttacaagtgc
    18481 ttcaccatga ccaacttttg gaagatcatg ctcaacaact tcaacacctg tactagcttg
    18541 attaacaaca accgctttca tgataaaacc tcctagaaat aaatatatct tattttgtaa
    18601 tcgtttacat tttatattgt attatgcttc acaaactatg tcaagtgatt ttcagaaaat
    18661 tttttgaaaa tattcttaac tggttaatct ttttgaaact aaaaatccca ctcaatagag
    18721 tgagattctt cgactgttgt tacttacggc gtcctggacg ttcctcataa ccataataag
    18781 catcttcgat gatttcttgc atgtgatcta ccattggaag gcgtggattt gctggtgaac
    18841 attgatcttc atatgctaag aaagctagtt cacgagattt ttctttccat tctttttcat
    18901 caatgccttg gtcacggaaa ttcatcttaa tacctaaacg tgttccaagg tcatacacag
    18961 cttttgcata tgattcgaca gcttcttctg gtgttgctgc tggtaagccg agtaatttag
    19021 cgatatcttg atacttttca tcagcacgat agtaattgta cttaggccat gttgctgttt
    19081 tagctggacg agttccatta tagcggataa catacggcaa aagaattgca tttgtacgtc
    19141 cgtgtacggt atggaattgt gcgccaattt tatgcgccat tgagtgagaa atacctaaga
    19201 atgcattggc gaaagccata cctgccatag ttgacgcatt gtgcatcttt tcacgtgctt
    19261 caaaatcagc atcttttact gagcgttcta agtagtcaaa cacaatttta attgcttgaa
    19321 gagcaagtcc gtctgtataa tcatttgcca tctgtgaaac ataagcctct gttgcatgtg
    19381 tcaatacatc catacccgta tcagccgcaa taaaaccagg aacagtcata acaagggccg
    19441 gatcaacgat agctacagtt ggtgttaatg aataatcagc gattggatat ttacgattat
    19501 ttgctttgtc agagataacg gcaaatggtg tcacttcaga acctgttcca gaagttgttg
    19561 gaatagcaac aaattttgtt ttcttaccaa gttctgggaa tttgaacgca cgtttacgga
    19621 tatccatgaa tttttgaacg aggtcatgga aatcaacttc tggttgttca tagaagagcc
    19681 acataacttt cgcagcatcc ataggagaac caccacctaa tgcaataata gtgtctggtt
    19741 tgaaggtacg catcaaatct gttcctttca ttactgttgt aatgtctgga tctggttcta
    19801 cttcagcaaa aatctgataa acaactttat tgcgacgaag atcaagttgt tcaatgatac
    19861 gatccaagaa accaagctca accatggcgt gatctgtaac aatcatgaca cgttcaacat
    19921 cgcgacattt ttgcaaatat tgaatagaat cacgttcgaa gtatgttttt gaagggactt
    19981 taaaccattg catattattt ctacgtcttc ctactttttt gatattcaag agatttatag
    20041 cactaacgtt atcaccaact gagttacgtc catacgaacc acaaccaaga gtcaatgatg
    20101 gcaagaaagc attgtaaaca tccccaatac caccaaaagt agaaggagaa ttccaaataa
    20161 cacggatagc acgaattcta gttccaaatt cacgagctaa gtctgcatct tttgtatgaa
    20221 tagcagctga gtgccctaga ccattaaact caaccatttg acgagctttt tcaacaccat
    20281 cttctgtaga ttcagctttc aatactgcaa taacaggtga tagtttttca cgtgtcaaag
    20341 gttctttttc actaacttca gtacattctg cggcaagaat atttgttcct tctggaacag
    20401 taaatccagc ttgttcagca atccaaactg ctgattttcc aacaatattt ggatttaatt
    20461 ttgcaccagc acagtttttg ctatttgctt ttgcaccaaa acagaattct tcaagaagag
    20521 ctttttcttt cttattaacg aaataggtat gataagattt aaattcttca acaaattctt
    20581 tataaatttc tttatcaata ataacagcct gttctgatgc acataccata ccgttatcaa
    20641 atgatttaga cataacaata tcgtgagcgg cttgtcggat attagctgat ttttcaacat
    20701 aagctggaac attcccagca cctacaccaa gggcaggttt cccacatgag taggcagctt
    20761 taaccatcgc attaccacct gtcgcaagga tagtggcaat accatcatgg ttcatcaaag
    20821 cattagttgc atcaatcgat ggttgttcaa tccattggac acaattttca ggagctccag
    20881 cagcgatagc agcatcacga acaatacgtg ctgcatgtgc tgatgattct tgtgctgatg
    20941 ggtggaaggc aaaaataatt ggattacgtg ttttaagaga gattaatgac ttgaaaatag
    21001 cagttgaagt cgggttggtt gttggcgtaa taccacagac aactcccact ggttcagcaa
    21061 ttaatgtcaa gcctgtgaca tcgtcttctt caataactcc cactgtcttt gtatgacgca
    21121 tgttgttaac gacgtgttca catgcaaata aattttttgt cgctttatct tcaaagacac
    21181 cacgaccagt ttcctctact gcatgaagag caagttctcc atgggcatcc aaggctgcaa
    21241 cagaagcttt agctacaata taatcaactt gttcttgatt aagttgacgc atctcatcaa
    21301 gtgcttttaa accattttga accaatgtat caattgcaag agcaacatct gtagtttcta
    21361 ctgctttagt tttttcagtc atttgagact ccttttgatt tgttaaatgt ttcacaaatt
    21421 tgtttacgag attattatag catatttaaa aactttgtaa atagtttcac aacttttttg
    21481 tgaacttttt ttcaatcttt cggaaacatt tcttgaaagc gctttaatta tatttgtttt
    21541 ttcattacta attttcagat taaagccaat cgatttcatt tgtatcacca taaacggagg
    21601 tatctattat tcgttttcac aaaaaaagcc gagtacatga ctcggacttg tttattttgc
    21661 agctcgttga gctgattttt gaatagcttt tttgacgtta ctaacatata aatcagcccc
    21721 tgtagtatcg ctatcactat aatgcacacc atctgtattt ccccagattt ctggatgttc
    21781 tgtcgcaacc ttataccagt cagcaaccgt tatgtaatta tattttttca ttagcgagag
    21841 ttcataatca cgcactgtcg taacttgact catattttta gcattataag gtgttactat
    21901 aatcaaacgg tgtcccttag gcaaagattt aataaattgg ctcaagtctg tcttgtagtt
    21961 atctaatgaa ttcacaccaa ctgccaaaac aacaattttg gacaaggctt tattttgaat
    22021 acggttttca aaaatatcaa atgatttact aaaattacga ctcaccgcag catctaattg
    22081 tacttctggt aacaatttat taaaagcagc acttgacctt aaggcaactg aatctccgat
    22141 tactgtcaca tcactaagag cacctgcatc ccctgcggct agtgtatgtg ttcggttagt
    22201 atttgttcta gcttgttgta acgaattctg caataattct gtctcaaaac tacctatact
    22261 tggagctgtc aacattgtca ctacagtaat aagagttagt acccctccga tactaaataa
    22321 ccactttttg taaggcaaca agtcaaattc ataatcaagg aatttaggtt ttctacctaa
    22381 aataaaaggt tcaatgacat aaaacgataa tgttgagaag actgctgata atactactgt
    22441 caaaataaca gctatccagt ttggggataa acgactaaaa ataatataaa atggccaatg
    22501 aaaaaggtac ataccgtaac tgatatcagc aagataagta atcacgaaag gttctgaaat
    22561 atctggtgta tgctcgtgta aaatacgtgc attgtaaatc attacactag caaaaatgct
    22621 agacaataca aaaccaaaaa gatatgttag ccgattatca aaatcaagtg caaaagttaa
    22681 aatcattaga ccagcaaaag cactacccat catgattaaa ttatgcttca aagtcagatt
    22741 tttgatattt ttcttgaatc gtccggtgat ctctcgaata ccagatattg tagccaccat
    22801 cgctcctaaa aagaacggaa atatatgtga taatgtagag aaatatatcg ttgaaaaatt
    22861 atcaacgaaa aaggctctaa caaacatggt aaggaagctg actccaaaaa ctcccatgga
    22921 aataaggaac aaagtccccc tcaattgctt ttgatctttt gatctttttg ataagagcca
    22981 taccgttaag ccccataaaa cataaaaatg gacttcaata gaaagactcc aggtatgcac
    23041 aaataagtga gggataaatt gactttcgta gttaccacca gtcaagattt cgtaaaagtt
    23101 gctggtaaaa cctaaagcag tcataatttg agagccaata cttgctctaa aatcgctttt
    23161 aaccagaaat acaaaaggta ttgtcagcaa aaccatcaat actaatggtg ggaaaatacg
    23221 gtaaaaacgt ctacgacaaa aactaacaaa atcaattttt ttcgttttag aaaattcatc
    23281 aatcaacaag gccgtaatca gaaaaccaga aaatgtaaag aagatgtcaa caccaacaaa
    23341 tcctcctgga aaactattct taaagaaatg gtaaagtaaa acaagtagta gtcctgttat
    23401 acgtaccaaa gaaaaccatt ttattctcat ttttgataaa tcccctgttt aaatgtcccc
    23461 ttatagactt tcttattttt agcattgagg cgaccttgtc catcaggttg acctttttta
    23521 aagtcacctg tataagacca gccatgaact gaaacaaatg ttcctttccc ttcaaagacc
    23581 ccatttacaa aatcgccctt atagtaatcc ccattctcat aggttaattt acctttacca
    23641 gtcatatggt gattaacaat agagcccaca tattttatct taccgccatc atagctaagg
    23701 actccttgac tagaagtcgt taaggtaaat actgataaac caaaaacaac aataattatc
    23761 aaagataata tttctagatg agttcgtgtt attttaaaat tcttcatttc tacttaatct
    23821 ctactattat ttattagcgt taaccactgt ttacagccat cattgactag acgataggtt
    23881 tcctcaaaat caccagtata ccatggatca ggaacaccac catcacaaaa aaggctaatc
    23941 ttatcatcat aggttccttg tgatagagct tttaaatcct tgacattttg ctcatccata
    24001 ccgatgatat aatcaaaata tgctaaatcg tctttagaaa taggttgtga ccctttatcg
    24061 ttagtgtaag gaatttgata acgctgcaaa atcgcttgcg ttcccctatg tataggattt
    24121 ccatgctccc atgttgaagt cgcacgactc tcaaccgaga ggctatcctc cttatctaaa
    24181 ttcgccataa caaactcagc catagggcta cggcagatat ttccaagaca aacaaaacag
    24241 actttcttca tagcaaactc cttttggtct attataacag aaaaaaggta cttataatac
    24301 gtttaactgc tcaaagaata actacaacat atctaatact taacattgca tgaaaattaa
    24361 aaaattaaga ctagataccc tagccttaaa atgacttatg ttttatttat caaatcgctg
    24421 ataacctaaa tgttcgtatg ccttatccgt agctacacga ccggtacggg tacgcataat
    24481 aaaacctttt tgaattaaat aaggttcgta catgtcttca acagtatctc gttcttcagc
    24541 aatattaacg gatagagttc ctaaaccaac aggacctcca ttatacattt caatcatggt
    24601 tcttaagatt ttttgatcga cgtaatcaag cccctcgtga tcaacatcta acatcgttaa
    24661 tgctttatct gtaatattgt catctatcaa accatctccc atgatttgag cataatctcg
    24721 aacacgtttc aataaacggt tagcgatacg tggcgttcca cgactgcgac gcgctaattc
    24781 agaagcagct tcataagtaa ttttcatttc aaaaatatct gctgtacgct caataatttc
    24841 tgtcaaatcg ttttcttcat aatactccat atgccctgta ataccaaagc gagcacgtaa
    24901 gggattagat aacatacctg cacgtgtcgt tgcaccaatt aaggtaaagg gcggcaaatc
    24961 taaatgaaca cttctactag tttctcctgc accgatcata atgtcaatat aaaagtcttc
    25021 cattgcacta taaagtacct cttcaaccgc catcggcata cgatgaattt catcaataaa
    25081 aagaacatca cctggttcta aatcatttaa aatggctact aaatcccctg atttttcaat
    25141 tgcgggacct gatgtttgtt tgagattgac acccaactca ttagcaatta caaatgccat
    25201 ggttgttttc cctaaaccag gagggccaaa taataacaca tgatccaatg actcatcacg
    25261 caatttagca gcttcaataa atatttttag ctgatcttta accttatctt gtccaatata
    25321 ctctcttaaa tactgcggac gaagtgtacg ttctaccaat tcttcgtcac ccattgcatc
    25381 actatctaaa aatcttgtca tgatattatt ataacattaa acatttagaa ctgtatagag
    25441 gagtaaagca tatgttcagt gaactactac tatccccacc agaaaacccc agtcattact
    25501 gtaaaaaata ctttaaacat ctacttacct ctttctctta atctactgta agtataaatt
    25561 taaaaataaa aaactaacat g
//
LOCUS       DATRSD010000021        25555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRSD010000021 DATRSD010000000
VERSION     DATRSD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.021"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            53..274
                     /locus_tag="VBD85_000692"
     CDS             53..274
                     /locus_tag="VBD85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133259.1"
                     /translation="MTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQ
                     FQAEISKNIEDVRQGKFYTSEEVRAELGL"
     gene            264..599
                     /locus_tag="VBD85_000693"
     CDS             264..599
                     /locus_tag="VBD85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO7133260.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIIND
                     IEKLEVFPEVGFDADEKYGSEISNYHSTRGYTLSKDYIVLYHIEEEENSVVIDYLLPT
                     RSDYMKLFK"
     gene            complement(904..1374)
                     /locus_tag="VBD85_000694"
     CDS             complement(904..1374)
                     /locus_tag="VBD85_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133261.1"
                     /translation="MTDKKIVYYFDHDLSKKDMGGVGPTYTGKNLLFLLWWTYSYVAE
                     DNLDIDTSLFEVPEFNDFYNNGLELYRIKNELANYNIRFGYEVNPKDSTKMLIGILPT
                     DEEDYVKGYRNFIADISNPDTRYAIYHRLIKTMDTMLPIEQINVYKSLAKGNKK"
     gene            complement(1542..2798)
                     /locus_tag="VBD85_000695"
     CDS             complement(1542..2798)
                     /locus_tag="VBD85_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEO7133262.1"
                     /translation="MSYVVARMAKYKSGQLTAIYNHNERIFKNHSNKEIDVEKSHLNY
                     ELTNRDQAQNYHKQIKEHINENRLSTRGVRKDAILCNEWIITSDKTFFNSLDEKQTRE
                     FFDTAKDYFAEKYGDANIAYARVHLDESTPHMHLGIVPMKNGKLSSKALFGNKEKLVA
                     IQDELPKYLNEHGFNLQRGEIGSKKKHLETAEFKEKQRLLDNADRKLADKHEELKALD
                     DKISNVNDTIADKESRLKELEAKEWDAVGDLKQYELEKQSLAESIEDIKDIELLQLDR
                     IQKEDLVKQSFDGKLKMDKETYNRLFQTASKHASSNAELKRDLVKAQSQNNHLSRELL
                     NHRKTAEKNIKLSQENRKLKDKVKMLDEQVKILNKSLSVWKEKAKEFMPKQVYRETLS
                     IINTLNPIGLAKTAIRQVKKMVDSNS"
     gene            complement(3115..3549)
                     /locus_tag="VBD85_000696"
     CDS             complement(3115..3549)
                     /locus_tag="VBD85_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133263.1"
                     /translation="MRIKDYADSLGVSSQSIYKRIRSPKYKERLKGHLYRDNQKVENL
                     DLIGIKILEDYHFENDVIELEKTLGDIQEEFEQEKKGMQYRIDRLADKLTPLLEDNQN
                     LVQKNYELLNYVRSLERQKLLLIIALAVMVITLLVAIWLAIF"
     gene            complement(3585..4460)
                     /locus_tag="VBD85_000697"
     CDS             complement(3585..4460)
                     /locus_tag="VBD85_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO7133264.1"
                     /translation="MELKVSLDNITITAYIKSYKLLGLKRLVEGHTAIIIQTAMTDMF
                     RAYTKDGGQVVLHMEYDKIKGQAFNARPFRLEFNPNKLRSVDTEIIDTIIPYLEDISI
                     SRADLAFDLFEVDCSEFVLEKKGRPTATKEFRSDTGKLETKYLGASRSEKQVRLYNKK
                     KEQLENGTDKEKEFASQFKHWWRLEFQLRSRSVEEIFEVINTVVFKPFKFEGLPVETQ
                     IYLVALTRDKNLWKQLHRNTRAKYKKILETYQTSDIDYLGLLKDLLKHERLRLENQLA
                     YYGGRIDKNSFQPFC"
     gene            complement(4760..5401)
                     /locus_tag="VBD85_000698"
     CDS             complement(4760..5401)
                     /locus_tag="VBD85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133265.1"
                     /translation="MIKIYFGKDAALNQAIQSRLDSYQIDYQAFSSKDIDAKTLMEWL
                     FKSTDIFELLSTKMLKYKLNTQITLSQFVRKILKDVNSTLKLPIVVTDEVIYSNMSPD
                     YVTVLLPKEYRKIKRIQLMRKMEQLDEGRLFWKNFELFRKQSELRWFELNELLFADVS
                     DDLGEIKKAKDRFFSYKKNNQVPPNEIIERILKIFLVDREDFFKKSPSDLQNF"
     gene            complement(5405..5731)
                     /locus_tag="VBD85_000699"
     CDS             complement(5405..5731)
                     /locus_tag="VBD85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO7133266.1"
                     /translation="MDYDNENYLIPKILLQDDFYSSLSAKDILVYAVLKDRQIEALEK
                     GWIDTDGSIYLNFKLIELAKMFSCSRTTMIDVMQRLEEVNLIERERVDVFYGYSLPYK
                     TYINEV"
     gene            complement(5737..6018)
                     /locus_tag="VBD85_000700"
     CDS             complement(5737..6018)
                     /locus_tag="VBD85_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133267.1"
                     /translation="MTEGFTIQLPKVTEKKLLARYDDMLQKAIEKALEDKELYKPIVR
                     MAGLCRWLDVSTTTVVKWQKQGGMPHMVIDGVTLYDKHKVAQWLQQFER"
     gene            complement(6159..6452)
                     /locus_tag="VBD85_000701"
     CDS             complement(6159..6452)
                     /locus_tag="VBD85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133268.1"
                     /translation="MAYTLEERETIVRYDEMDKCWYFESNVRRHVTKILKMEHAFDEI
                     EKELENDFCIYVRAKLSDLNNFSVNPFVKNKRKLSDEQRLELSRLAKKRFSSD"
     gene            6617..7168
                     /locus_tag="VBD85_000702"
     CDS             6617..7168
                     /locus_tag="VBD85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004235211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7133269.1"
                     /translation="MNTRIPYLVALSGKTLKQISEETGIAYPTLTSYNQGVRNPKKNN
                     AVILAKYFGVSVGYLLGDEKEPNLINPGDESLIKGLFRVAQGKTNLDKVTEWTPFKDD
                     VSFVKTMLLSTPTLERKIDEIFEGIEPNIIQALKQRIKEVAEEFPYTINHSGDEKSDY
                     HFVWQAWEESDEYKKRMAEKHKK"
     gene            7237..8391
                     /locus_tag="VBD85_000703"
     CDS             7237..8391
                     /locus_tag="VBD85_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7133270.1"
                     /translation="MSIHKYPSKKAKNGYLYLVKVYMTRDGQRADHIKRGFRTRKEAK
                     DYEARLIYLKASGKLEEFIKPTHKTYNEIFEKWYQAYQDTVEPTTASRTLDMFRLHIL
                     PVMGDLPISKISPLDCQNFIIEKAKVFKNIKQIKSYTGKVFDFAIKMKLLKHNPMTEI
                     IMPKRKKTRIENYWTVQELQEFLAIVLQEEPYKHYALFRLLAYSGLRKGELYALKWAD
                     IDFQSETLSVNKSLGRLDGQAIEKGTKNDFSVRRIKLDSETISILQEWKANCQKEKAQ
                     LAVAPLSIEQDFIFTYCTRSGSIEPLHADYINNVLSRIIRKHGLRKISPHGFRHTHAT
                     LMIEIGVDPVNTAKRLGHASSQMTLDTYSHSTTNGEDKSIKQFADYLKAK"
     gene            complement(8519..8911)
                     /gene="rpsI"
                     /locus_tag="VBD85_000704"
     CDS             complement(8519..8911)
                     /gene="rpsI"
                     /locus_tag="VBD85_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO7133271.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8932..9378)
                     /gene="rplM"
                     /locus_tag="VBD85_000705"
     CDS             complement(8932..9378)
                     /gene="rplM"
                     /locus_tag="VBD85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO7133272.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDAADVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(9600..9722)
                     /locus_tag="VBD85_000706"
                     /pseudo
     CDS             complement(9600..9722)
                     /locus_tag="VBD85_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10259..11119)
                     /locus_tag="VBD85_000707"
     CDS             complement(10259..11119)
                     /locus_tag="VBD85_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7133273.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(11212..11730)
                     /locus_tag="VBD85_000708"
     CDS             complement(11212..11730)
                     /locus_tag="VBD85_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO7133274.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(11727..12482)
                     /gene="rlmB"
                     /locus_tag="VBD85_000709"
     CDS             complement(11727..12482)
                     /gene="rlmB"
                     /locus_tag="VBD85_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO7133275.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKTLSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKEFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12585..12971)
                     /locus_tag="VBD85_000710"
     CDS             complement(12585..12971)
                     /locus_tag="VBD85_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO7133276.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(12964..14307)
                     /locus_tag="VBD85_000711"
     CDS             complement(12964..14307)
                     /locus_tag="VBD85_000711"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO7133277.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     FGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQGAMDDDFNTANGITVIFEMAKWINSGHYTSRVKETFAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(14304..14486)
                     /locus_tag="VBD85_000712"
     CDS             complement(14304..14486)
                     /locus_tag="VBD85_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO7133278.1"
                     /translation="MKDYVTTQILSILGILAAACLSYLKFAQGNPLLGIIWAAFVLMY
                     VVRLYKAYQKQKKEKP"
     gene            complement(14496..15080)
                     /gene="cysE"
                     /locus_tag="VBD85_000713"
     CDS             complement(14496..15080)
                     /gene="cysE"
                     /locus_tag="VBD85_000713"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO7133279.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(15089..15841)
                     /locus_tag="VBD85_000714"
     CDS             complement(15089..15841)
                     /locus_tag="VBD85_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO7133280.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRLFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(15843..17972)
                     /gene="pnp"
                     /locus_tag="VBD85_000715"
     CDS             complement(15843..17972)
                     /gene="pnp"
                     /locus_tag="VBD85_000715"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO7133281.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKIRPDGRRVDEIRPLDAEI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAEPRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(18353..18622)
                     /gene="rpsO"
                     /locus_tag="VBD85_000716"
     CDS             complement(18353..18622)
                     /gene="rpsO"
                     /locus_tag="VBD85_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO7133282.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(18710..19969)
                     /locus_tag="VBD85_000717"
     CDS             complement(18710..19969)
                     /locus_tag="VBD85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO7133283.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLGQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLAYILGLVHTFMILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLKGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(20078..21007)
                     /locus_tag="VBD85_000718"
     CDS             complement(20078..21007)
                     /locus_tag="VBD85_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO7133284.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNALAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGISAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGREDFSKGFIQLRQGKDITLVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVQHCKSIYKS"
     gene            complement(21004..21861)
                     /locus_tag="VBD85_000719"
     CDS             complement(21004..21861)
                     /locus_tag="VBD85_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7133285.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVEAL
                     AVLYGDIMDINPEKFKESDRDYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVRPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERALIS
                     LRESLEVVE"
     gene            complement(21864..23219)
                     /locus_tag="VBD85_000720"
     CDS             complement(21864..23219)
                     /locus_tag="VBD85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO7133286.1"
                     /translation="MKGLLDFLVNIASTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGTGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGGLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(23232..23516)
                     /locus_tag="VBD85_000721"
     CDS             complement(23232..23516)
                     /locus_tag="VBD85_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133287.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(23519..25555)
                     /locus_tag="VBD85_000722"
     CDS             complement(23519..25555)
                     /locus_tag="VBD85_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO7133288.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGG
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDILRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRRYQDIEMIIKQED"
BASE COUNT         8008 a         5219 c         4086 g         8242 t
ORIGIN      
        1 aaaaaataga gctgtcacat tccaagctaa caaagaattg gtaagcgaag caatgacagt
       61 attaaacaaa aaaaatttaa ccttatcatc tgctttaaga ttattcctac aaaatgtcgt
      121 tgtcacaaac gaggttgact tattgacgga agaggagtta gagaaagaaa aacttttcaa
      181 gcaatttcaa gccgaaatca gcaaaaatat tgaggatgtt cgtcaaggga agttttatac
      241 ctctgaagaa gtgagggctg aacttggact ataagaaata tcagattatc tatgctcctg
      301 atgttttaga gaagctaaaa gaaattcgtg attatatttc ccaaaactat tcctcaacat
      361 caggtcagcg taaaatggag caaatcatta atgacataga aaaacttgaa gttttcccag
      421 aagttggttt tgatgccgat gaaaaatatg gttcagaaat cagcaattat cacagtacta
      481 gaggttacac cttaagtaaa gattatattg tcctatacca tatcgaagag gaagagaata
      541 gcgttgtcat tgattacttg cttcctactc gaagcgacta tatgaaattg tttaaatagc
      601 aatcaaaaag ccttagcgta atgccaaggc tttttgaaga ttacaaatta actaggagca
      661 aaattagttc gggctgttta gtagatattc cacaaggcag agccaaccct gtattgataa
      721 acaacacctt tgagaaaact gttaccagtc aatcaacctt ctcctaatgt aaaagctgag
      781 aacccagtcg gggatttacc taagtatact caggaggcaa cacctctacc taataccttc
      841 aataagtatt ttttgaatga attgttacca attcaaactc agtctcaact ttcttccact
      901 ctatcacttt ttattacctt ttgcaaggct tttataaaca ttaatttgtt caatcggtag
      961 cattgtgtcc atggttttaa ttagtcgatg atagattgcg taacgtgtgt caggattgga
     1021 aatatcagca ataaaatttc tatatccctt aacataatct tcttcatcag ttggcaaaat
     1081 acctatcagc atctttgtac tatcttttgg attaacctca tatccaaatc tgatattgta
     1141 attagctagt tcatttttta tacgataaag ctctagacca ttattgtaaa aatcattgaa
     1201 ttctgggact tcaaacaatg acgtatcaat gtctaaattg tcttctgcca cataagaata
     1261 tgtccaccaa agtaggaata aaagattttt accggtgtag gtaggtccta ctcctcccat
     1321 atcttttttt gataagtcat ggtcaaagta ataaacaatt tttttatctg tcattttaat
     1381 ttctccttac tcatttattc catggttctc ttgacaacaa gtaacctaga ctgttttagt
     1441 ctatggcgat cggtcgccag ttctttagcc tgacgactcg accccatcta gtctaaactt
     1501 gcctagcttg ttatcaagag actttcacgg cataaaagta gctaactatt actatcaacc
     1561 atcttcttaa cttgtctgat tgctgtttta gctaaaccaa taggatttag agtgtttatg
     1621 attgataatg tttctctata aacctgttta ggcataaatt ctttagcttt ttctttccaa
     1681 acagagaggc ttttattcag aattttcact tgttcatcaa gcatttttac tttgtccttt
     1741 aattttctgt tttcttgact taatttgata tttttttcag ctgtcttacg gtgattgagt
     1801 aattctctag aaaggtgatt gttctgcgac tgggctttaa ccaagtctct ttttaactca
     1861 gcattactag atgcatgttt cgaagcagtt tgaaatagtc tattataggt ttctttatcc
     1921 atcttcaatt tgccatcaaa gctttgtttt accaaatctt ctttttgaat tctatcaagt
     1981 tgtaatagtt caatgtcttt tatgtcttca atgctttcag ctagtgactg tttctctaat
     2041 tcatactgct tcaaatcgcc aacagcatcc cattctttcg cttccaattc tttaagccta
     2101 ctttctttat cagctattgt atcgttaaca tttgaaatct tatcatcaag tgctttcaat
     2161 tcttcatgtt tatcagctag ttttctatca gcgttatcca gtaatcgttg tttttcttta
     2221 aattcagcag tttccaaatg ttttttctta ctgcctattt cgccacgttg taggttaaag
     2281 ccatgctcat taaggtactt gggaagttca tcttggattg ctacaagttt ctctttattt
     2341 ccaaacagtg cttttgaact taacttacca tttttcattg gcacgatacc taaatgcata
     2401 tgcggagtac tttcatcaag gtgaactcta gcgtaagcaa tattagcatc accatatttt
     2461 tcagcaaaat aatctttagc tgtatcaaaa aactctcgtg tctgtttctc atctaacgag
     2521 ttaaaaaacg ttttatctga agtaataatc cattcgttac agagtatagc atctttacgg
     2581 actccacgag tagataagcg attttcattg atatgctcct ttatttgttt gtgataattt
     2641 tgtgcctggt ctcgatttgt cagttcataa ttaagatgag atttctcaac gtcaatttct
     2701 ttatttgagt gatttttaaa aatccgttca ttatgattat aaatagccgt caactgacct
     2761 gacttgtact tagccattct agcaacaaca taactcattt tttaattctc ctttctcggc
     2821 tcaaaaggtc gccgagatgg tggcggtaat tgccaccatg aataagcaac ctattatctg
     2881 gatttcttct taagggatcg gcataacttc catgtaaagt gtaacgcact acactttact
     2941 acgtaaatgt gcgttctccg aagggtcttg cagacagctc ttcgctccga atacaggagc
     3001 gacttcgaac cacctctcga gccacaagag gagcttcgat tatactcgcc gaagcgagta
     3061 gggcacttaa gcagtgccct tggtcggacg cttcgcattg tccgacaatt gccatcaaaa
     3121 gatggcaagc catattgcca ctaataaagt gattaccatg acagctaaag caataatcag
     3181 taatagcttc tgtcgttcca agctccgaac atagttgagt aactcataat ttttttgtac
     3241 caagttttgg ttatcctcaa gtaaaggagt taacttatca gctagtctat ctatccgata
     3301 ttgcattccc ttcttttctt gctcgaattc ttcttgaata tcacccagcg ttttttccag
     3361 ttcaatgaca tcattctcaa aatgataatc ttcaagtatc ttaatgccta tcaaatcaag
     3421 attttcaacc ttttggttgt ctcggtataa atgacctttc aacctttctt tatattttgg
     3481 agagcgtatg cgtttataga tagattggct actaactcct agactatcag cataatcttt
     3541 tattctcatt tttgacctcg taactccttg ataatactga attttcaaca aaagggttga
     3601 aagctatttt tgtctatcct acccccataa taagcgagct gattttctag tcttagtctt
     3661 tcatgcttca acaagtcttt cagcaaacct aaataatcaa tatctgatgt ttgataggtt
     3721 tctaaaatct ttttgtattt tgctcgtgtg tttctgtgta gttgcttcca gagattttta
     3781 tctcgtgtaa gagctacaag atagatctga gtttcaacag gtaaaccttc aaatttgaat
     3841 ggtttaaaaa caacagtatt gataacctcg aatatctcct caactgaacg acttcttaac
     3901 tgaaattcca atcgccacca atgcttaaat tgacttgcaa attccttttc tttgtcagtc
     3961 ccattttcta gttgctcttt tttcttgtta tacagcctaa cctgtttttc cgagcgagaa
     4021 gcacctaaat acttcgtttc tagttttcca gtatctgaac gaaattcttt ggtcgcagtg
     4081 gggcgccctt ttttctctag cacaaactca ctacaatcaa cctcaaacaa atcaaaagct
     4141 aagtcagcac gagatattga aatatcttca agatatggta taatagtgtc tataatttca
     4201 gtatctactg aacggagttt attagggttg aactccaaac ggaaagggcg agcattaaag
     4261 gcttgtcctt ttattttgtc atattccatg tggagcacaa cttgacctcc atcctttgtg
     4321 taagctctga acatatcagt catagcagtt tgaataataa tagctgtatg accttcaacc
     4381 aatcttttta agcctaggag cttgtatgat ttgatgtaag ctgttatcgt aatattgtct
     4441 aagctgactt ttaattccaa acaagactcc tttcttttct tggtcgccta tcggcgccaa
     4501 tttttttgac tgcgtaaagg cctacgtaaa atgacattct cgggggagca accccgagcc
     4561 gaccccttcg gagtcgaacc attttcattt tgctccagcc aaacttgcca aaaaacgggt
     4621 gtagtaaatg cacatcaaat gtgcacatag cgggctgtta gagggcgccc tgttataaat
     4681 ttttcaaaac ccctatagat cggacctcaa atctccgttt tcaaaatatt ttcacaactg
     4741 ttttcataac tatttttgac tagaaatttt gtaagtccga aggagatttt ttaaaaaagt
     4801 cttctcggtc aaccaaaaat attttgagga ttctttcgat aatctcatta ggaggtacct
     4861 ggttattttt cttgtagcta aagaaacggt ctttagcttt tttgatttct cctaaatcgt
     4921 cggatacatc agcaaacagt aactcattca gttcaaacca acgtagctct gattgcttac
     4981 ggaataattc aaagtttttc cagaatagac gaccctcatc taactgctcc atcttcctca
     5041 tcagctgaat tcttttaatt ttccgatatt cttttggcaa gagtactgtc acatagtcag
     5101 gggacatgtt cgaataaata acctcatctg tcacaacaat gggaagcttc aaggtgctat
     5161 tgacatcttt taaaatcttt cgaacaaatt gactgagggt aatttgtgta tttagcttat
     5221 acttcaacat cttagtactt agtaattcaa atatatccgt tgacttaaac agccattcca
     5281 tcagtgtttt agcatctata tctttacttg agaatgcttg atagtcaatc tgataactat
     5341 ccaatcgcga ctgaatagct tggttaagtg ctgcatcttt tccaaaataa atctttatca
     5401 ttgcttatac ctcgttgata taggttttgt agggtaaaga gtagccataa aaaacgtcta
     5461 ctctttctcg ttcaatcaaa ttaacttctt ccaatcgttg cataacatct atcattgtgg
     5521 ttctgctgca agaaaacatt ttagctaatt caatcagttt aaaatttaga tatatactgc
     5581 catcagtatc tatccaacct ttttcaagag cttctatctg tctatctttc aagacagcat
     5641 agactaaaat atctttcgca gatagactac tgtagaaatc atcttgtagt aaaatcttag
     5701 gtattaagta gttttcgtta tcatagtcca taactcttac ctctcaaatt gttgtagcca
     5761 ttgagcgacc ttgtgcttat cgtacagggt cacaccatca atcaccatgt ggggcatccc
     5821 tccctgtttt tgccatttga caacggttgt cgttgagaca tctagccaac ggcacaagcc
     5881 agccatacga actataggct tatagagttc tttatcctca agggcttttt ctattgcctt
     5941 ttgaagcatg tcatcatagc gagccagtaa cttcttttca gttactttgg ggagctgaat
     6001 ggtaaatcct tctgtcattt tgagttctcc tttttgttaa aatcaatttt aagctctgaa
     6061 acgcttattg taatctgata tttcaggatt gaaatatctt cctagggtaa atataccaga
     6121 gaagattttt aagtctgttt ttgattgctg tacgcttcct agtcagacga gaaacgtttt
     6181 ttagcaagtc gacttaactc taatctctgt tcgtcagata gttttcgttt attctttaca
     6241 aacggattga ctgaaaagtt atttaaatca gacagttttg cacgaacata gatacaaaaa
     6301 tcattctcga gctctttttc gatttcgtcg aatgcatgct ccattttcaa aatctttgta
     6361 acgtgtcttc taacgttact ttcaaaatac caacacttat ccatttcatc gtaacggaca
     6421 atcgtttctc gttcttctag tgtgtaagcc ataactgacc cctttccatt ttttacaaac
     6481 tccgaaagca tttatttttg cattatttaa atacataata cattttaaaa tacatttgtg
     6541 tttatgtcaa tactttttgt accttaattt gtatttttgt gttatttgtg ttattttatt
     6601 tacaaaagga gatgaaatga atacaagaat tccatatcta gtcgctttaa gcggtaaaac
     6661 actgaaacaa attagcgaag aaaccggaat agcttatcca acattaacaa gctataacca
     6721 aggtgtacgc aatcctaaaa aaaataacgc agttattctt gcaaaatatt ttggagtatc
     6781 agttggatac ttattaggag atgaaaaaga gcctaactta ataaatccag gtgatgagtc
     6841 tctaataaaa ggtcttttta gagtggctca aggtaaaact aatctagaca aagtaactga
     6901 gtggacacct ttcaaagatg acgtcagttt cgtaaaaaca atgctccttt ccaccccaac
     6961 acttgagaga aaaatcgatg agatttttga aggcattgaa cctaatataa ttcaagcact
     7021 caagcaaagg ataaaagaag ttgccgaaga atttccttat acaattaacc atagcggaga
     7081 tgagaaatca gattatcact tcgtttggca agcatgggaa gaatcggacg aatacaagaa
     7141 aagaatggct gaaaaacaca aaaaataatc aaagcaatct gtaatttcct tattttacaa
     7201 gctccgaaac cttagaaaaa taaaggagaa attacaatgt ccattcacaa atacccaagc
     7261 aaaaaagcca aaaatggcta tttgtattta gtcaaggtct atatgacaag agacggtcaa
     7321 cgtgctgacc atatcaaacg tggctttcgt actcgaaagg aagccaaaga ttacgaagct
     7381 aggctaatct accttaaggc ttcaggaaaa ttggaagagt tcatcaagcc aactcataaa
     7441 acttataacg agatattcga gaaatggtat caagcctatc aagatacggt agagccgacc
     7501 acggcttctc gtactcttga tatgtttagg cttcacattc tgccagtcat gggagactta
     7561 cccatcagca aaatatcccc tttagactgc cagaacttca tcatagaaaa agctaaggta
     7621 tttaagaata tcaagcaaat caagtcctac actggcaagg tctttgattt tgctatcaag
     7681 atgaaactgc tcaaacataa ccctatgaca gaaatcatta tgccaaagcg gaaaaagaca
     7741 cggatagaaa actattggac agttcaggaa cttcaagagt ttttagccat agtgcttcaa
     7801 gaagagcctt acaagcatta tgcactgttc aggctactag cctacagtgg cttaaggaaa
     7861 ggcgagctat acgcccttaa atgggctgat atagattttc agagtgagac actaagcgtc
     7921 aataaaagct taggacgatt agacggacag gcaatcgaaa aaggaactaa aaatgatttt
     7981 tcggttcgaa ggataaagct agatagtgaa actatttcta ttctgcagga atggaaggca
     8041 aattgccaaa aagaaaaggc acaactagca gtcgcacctc tttctatcga gcaagatttt
     8101 atttttactt actgcacacg gtcaggctca atagagccac tacatgcaga ctatatcaac
     8161 aatgtactat cacggattat caggaagcat ggcttaagga aaatcagtcc tcacggtttc
     8221 aggcataccc atgctacctt aatgattgaa attggagttg acccagtcaa cacagcaaaa
     8281 cgattaggac atgccagtag tcaaatgact ttagatactt acagccattc aacaactaac
     8341 ggagaagata agtccatcaa gcaatttgcg gactatctca aagctaaata acagtcaaaa
     8401 aagatgtgaa agtttttcca aaacggtatc aaaaaaggta tcaaaagcaa aaaacgacaa
     8461 aaaaagcacc ttgcaagaga cttgcaaaat gcttgtaatc gttgatgtaa agcggttctt
     8521 aacgttttga gaactggcta gctttacgag ctttcttaag acctggtttt ttacgttcaa
     8581 ccatacgtgc gtcacgtgta agaagtccag cacgtttcaa tgaatcgcgg aaatctgggt
     8641 caacttctaa aagtgcacgt gagataccat gacggatcgc acctgattga cctgcatatc
     8701 caccacctac aacgttaaca aaaacatcgt atgaaccttg agttgaagta actgcgaaag
     8761 gttggttgat aacaagacga aggtctgcgt gtgggatgta ctcttctaca tcttttttat
     8821 tgattgtaat tttaccagta cctgggacca aacgaacgcg tgcaacagcg tttttacggc
     8881 gaccagtacc tgcatattgt gcttgtgcca ttatataatg ctcctttcct cttagataag
     8941 tcctgagata tcaagtactt ctggttgttg tgcagcatgt gtatgctcac cgccaacaaa
     9001 tactttcaat ttcatacctt gtgcacgtcc aagagtgtta tgtggaagca tgcctttaac
     9061 tgatttttca atcaaacgaa cagcattttt agaacgaagt tcacctgctg agatttgttt
     9121 caaaccacct ggatacattg agtgagtgta gtagatttta tctgacgctt ttttaccagt
     9181 taatttaact ttttcagcat tgataacaat cacaaagtca cctgtatcag tgtgtggtgt
     9241 gaaagttggt ttgttttttc cgcgaagtac gctagcaact actgcagaaa gacgtccaag
     9301 aggtacatct gctgcgtcaa caacgtacca tttgcgttca acttggcctg gtttagccat
     9361 gaaagttgtt ttgttcatga tttctcctat acgaattcgt ttttgtttac aggtgtggat
     9421 gttccggtcc acgagagtat ttgaaaggtt ccggggcctt acaaatgggg taaacaatac
     9481 cgcttataat catatcaaaa taaggtagca ttcgtcaagt tatttctttt attttatcaa
     9541 ttttcatccc aaaataaatc atctacagtt ttattcaaag cttgacacaa tttaatcgct
     9601 taattatagt ctcctttttc aatggcggct attgtctgac atgatacacc gaccttttca
     9661 gctagagact gttataataa atccatacta gctctagccg tctttaagcg taaatttttc
     9721 atattactat ctgtccttat acttatggta gtacactgaa attcccatag ctatcaatga
     9781 tattcctata actgctaaaa ttgcatcatc tccccttgaa aaataagtag caatatcaac
     9841 cttcctagta aaagcatcaa taaaatagtt attcctatga gggcaaatgc gccagcaaca
     9901 aaaacatttt ccaaaaactt tcatcaacaa acggagagac gctttttaga ttagaagtcg
     9961 taacaaaata gaaataccac taaataaagt taatattatt ataaaatcag gtaacacact
    10021 ccccttatta gctttccaaa aacactttca agccataaga taaaaaacat cgtctaaaaa
    10081 catttttgat aactttgtaa aaccaattgt tgttgtcgtt tatcatattg aggatagttc
    10141 ttattttatt ttttataata ggtctcctac tattttatag tttcatagca tcatataaaa
    10201 tacattttat aaaatgtata ctaaaagaac ataacgtact gttatgccct ttgatttgtt
    10261 atcggttttc ttcccctaaa gagaatagag ctaaagcacc tgagcccaca tgagctgaaa
    10321 taactggccc taatggacga ataataactt cttcaatacg ctcatctgct aataactgtt
    10381 ctttcataac ttgagctgaa tctttctctc cactataagc aataattaaa gtgctataag
    10441 atagtgtttt tgttgcctga gtcacaatct ctttaatacc ttttttacgg ccccttgtct
    10501 tggcaaatgg tactaattta ccctcactat ctaacttaag taatggttta atcttagcaa
    10561 cacttccaat gatagctgca ccttttgata agcgaccact acgcattaaa tggttgagat
    10621 cgtcaactaa aaagtatgtg tttaatttag ggagaagtga ttcaattttt tgtttcactt
    10681 cttctaaaga tttaccttct tgtcgctctt tagttgctaa catcgctaag acaccctcac
    10741 cacatgaagc tgccatcgta tccacaatct caatttgagc atcaggatat ttgtctaaga
    10801 ccatctcacg cgcaatagtt gcactctggt aagtgcctga taaatgtgaa gatagcgcta
    10861 aatataacaa ggcatggtca ttttctgcat aggttgaaaa aacttcttca aactgaccaa
    10921 cgttaatttg gcttgttgta ggctttgctc cctcttgcat acgctccaac aagaagtcac
    10981 tagtaatttt ttcgtctccg actgtctcat aagtctttcc atcaagttca atggttaaac
    11041 ctataatatc gacattgtgc tcttgagccc acttttcatc taaatccgat gtcgaatccg
    11101 ttagtatttt aaaagtcatt gttacctcaa tctattattt ttatttgata atcaaagtag
    11161 ttccaatatt atatcaaaaa aattgttggc tggctaataa agtgttaggt tttacatacc
    11221 atctaaaaaa tccttgagtt ttcctagttg ttcatcattc cacggcctta actttggcgt
    11281 actcaaatcg atttgactgg acatcttatc caaatctgat tttactgtcg ctacacgctg
    11341 ttccaattct ctagccgaga cacgtaaagc accctgagaa aatatagtcc attgttcatt
    11401 taaatcactg gtagcaacgg agactaaatt taacacagat tgattaagtt ctgccgctgc
    11461 tcgttcaata tagctatccg cagtttcgtc ctcctcagta aagataacgg atattttgta
    11521 ttggtcataa cgttgacgta caccaggaac atactgtgca tcaaatacac aaataatatc
    11581 aatgtgttcg aaatgtgcgt aatgatttag ctttctaagt aatacttccc ttgcctcttc
    11641 taaacgattg cttttaaaaa gctgtcgagt atctttccaa aaagcaatca tattgtagcc
    11701 atcaacgagt aagatagaat gttttttcat aggcggtttc taaatacttc atacatcaaa
    11761 atagcggctg cgacactggc atttaaactc tgaacatgac cattcatggg aattgtaatc
    11821 atttcatcta cttgcttttt gatattatga gaaatacctt ttccttcatt accaataact
    11881 agggctaatt tacctttggt attccactta tgcgatggtg tcccattcat atcagtgcca
    11941 aatatccaga actccttatc ttttaaagta tccaaagttt gactgagatt agttaccctt
    12001 gcaataggaa catgttcaac agctcctgta gatgtctttg aaaccactgg ggtcactcca
    12061 acagaacgat gtttggggat gataatacct gttacattgg ttgcatcagc tgtgcgtaga
    12121 atagaaccta ggttgtgagg gtcggtcaga ccatcaagga ttaaaatgag aggattttct
    12181 tcattctctg cctttgtcat tatctctgat aaatccgcat aggcaaattc cgaaactttt
    12241 agaacgaatc cttggtgaac tccaccattt gtcatgtctg ataaggtttt tttaggagtc
    12301 caggaaatgg atactttttt ttcagtcgct agtgctttga ctttatcaac gttctttcct
    12361 cttaagtcat cttgcaagta taatttatta ccagtattag ctcttaaact ctctgtcaca
    12421 gcatgtaagc catagacaag gtcacttgat tcttctttaa attgtttatc tttcatagtc
    12481 atattatatc atgaatgcta tagcataaag ctttttatag caatatttta ccaacaaatc
    12541 ctaatgtctc tataaaaaag agaggatata gcctctcttt ttgtttattt ttcaatggtt
    12601 tcaatacacc actgtattag agtttccaag cgctttatct gaccagtcat atccaagtac
    12661 cccataagag cctcaaaacc tgtagacata cgataggtaa taatatcagc atttttagct
    12721 tttgtatgac tattcgcatt acgtcctcgt ttataaatta actgctcttc atcagttaaa
    12781 atattttctt ctaacatggc attaattaac aatgcttggg cattagcaga aacatattgg
    12841 gttgctttcc gatgtaactg attaggtttg gtaaaaccct gcataattaa atggcgtcgg
    12901 atatacaaag aataaactgc atctccttca aaagctaaag caataccatt aatcaaacga
    12961 acatcaatca cgagtccacc tcacgccatc cttagtatct aacaatttaa ttccttgttt
    13021 tgctaattca tctctaatac gatcagcagt tgcaaaatca cgattagctc gtgcttcttg
    13081 acgttgttct attaaagatt caatatcagc atcaagaact tcctcttgga atacaatacc
    13141 aaaaatttct aataattcag caaaggtttc cttaactctt gaagtatagt gtcctgaatt
    13201 aatccatttt gccatttcaa agataacagt aataccgttt gctgtattaa aatcatcatc
    13261 catcgctcct tgaaatgctt tcacaaattg ctctaattct tcatcattag cattctcttg
    13321 gattggtaag ttgaaagtat tttttaaata tttaaggtta acttccgcat catgaacggc
    13381 cttttcagtg aaattcacgg gtttacgata ctgttgtgtc gctaagaaaa aacgtattac
    13441 ttgtccgtca acgctcttca acatatcgtg aacagtaata aagttaccaa gagatttaga
    13501 cattttctcg ttatcaacgt taacaaaacc attatgcatc cagtaatttg caaaggtctt
    13561 acctgtttta gcttcagatt gtgcaatctc attcgtgtga tgtggaaatt ctaaatcagc
    13621 tcctccacca tggatatcaa tagtatcacc aaagatctca gttgccataa cagaacattc
    13681 aatatgccac cctgggcgcc ctttccccca aggactttcc catgagactt ccccagattt
    13741 agctgatttc caaagtgcaa aatctaaagg attctcttta atctctcctt caccatcaac
    13801 acgaccactt gcaccaatct ctaaatcttc caaagtttta ttggctaatt ttgcataatg
    13861 atgtgattta cttactctaa aataaacatc tccattagct tcataggcaa attccttatc
    13921 aactaaaact ttaacaaaat caataatctc atccatatag tcaataactc gaggattttt
    13981 tgtagcaggt ttgacaccta atgcagcaac atcttccata aatgctgaaa taaacttatc
    14041 tgaaaaggat tttgtatcca tacctgcttc agctgcacct tttataattt tatcatcaac
    14101 atcagtaaag ttagaaatat aattgacttg gtaaccacaa tactcaaaat aacgacgaat
    14161 agtatcaaaa gctactacac tgcgggcatt cccgatgtgt atatagttat aaactgttgg
    14221 cccacagacg tacatgttga ctttaccttc attgagaggt ataaaatctt gtaaactgcg
    14281 agtcatagtg tcgtaaattt ttatcatggt ttttcctttt tctgtttttg ataagcttta
    14341 tacaaacgaa caacatacat taaaacaaaa gcagcccaaa taattcctaa gagagggtta
    14401 ccttgtgcaa actttaaata gcttaaacaa gctgctgcta gtatacctaa gattgataat
    14461 atttgagttg ttacataatc cttcacaaat acctcctaaa gtttggacga ataataactt
    14521 tcttctcgat catgttcaat acttcggatt tgaagatcat ccttttgacc atggacacgg
    14581 acaactttag ctggaacacc aactactgtt acgtctgctg gcacatctgc aaggacaact
    14641 gctgcagcac cgactttagc attttctcca acctcaattg gaccaatgat ttgagaatgt
    14701 gctgaaatta gcgctccttt acgtatggtt gggtgtcgtt tgcctttgtc tttacctgtg
    14761 ccacccaatg taaccccatg gtatagcata gcacctttct ctacaatggc agtttctcca
    14821 atgaccaatc ctgaaccgtg gtcaataaaa accccttccg aaatcgtcgc tccgggatga
    14881 atttcaattt gtgtccagaa acgccaaaat tgactgtgca tacgtgctaa caatttaaaa
    14941 ttatgattcc ataaaaaatg agatagccta tgagctgcaa gagcttttat tcctggataa
    15001 gttaaaataa cttctagaga actacgagca gctggatctt gttctttgac aatagctata
    15061 ctttctttcc accaccccat agttatcctc aatcgttttc tacatcagac caatcaacta
    15121 aaacttgttg ttcatcaaat ggattgatgg ctactccaaa ggtatcatca atctcatttt
    15181 ctccattacg cggtttataa atatcgtcaa tcgtccaggc taaaattact ccttgtgctt
    15241 tcctaaaagc accaccaaaa tcattatggt tataccattt ttcaaaactt aaaaggtttg
    15301 agaatactgg aacataactt tttccttcgg gggtagacat tgtagggaaa agacggtcaa
    15361 aactaccttc ttcacgtgga tggacaaagg caggtaccaa gtaggtttta tccatgatat
    15421 cggcaacaat gttatcttca tttaacaact gatttaagat tgtggtgtaa ttattcataa
    15481 attgcaccat atcttcacag tagaaaatag tcgagttccc aaaatcgcct tcttttttta
    15541 aattatagac caatcctgct aagccatgtg aaatagcttc atcaagaacg tctaaactac
    15601 gacgactctc ccaaaacata tctcgagcac tttcttgctc ctctttgaaa tgattaaggt
    15661 cagtaatatc cgtaaaaaca ggaacaactt cttgaccatc tacttgaata gcatagggtt
    15721 ccttgcttgc ccaaaccgta gagtggtgca aggcattcac aagacctata ctatctaaaa
    15781 aattatccgg tgcattgata aaagcacgca atctaatgtc taactcgttt gtacttgtca
    15841 tattaagatt cctttttagg attgtcagct tttggaggac gtggtaacaa tgctttcata
    15901 gaagcatcaa ctcgcccttt atcatcaatt ttgatgactt tcacatcaac ttcttcacca
    15961 atttcaagaa catcagctac atttgctgta cgtgtccatg caatttctga aatatgtacc
    16021 aatgcatctg ttttatcaaa gaggttgaca aaggcaccga atttctcgat acgaactact
    16081 ttagcatggt aaacttctcc cacttttgct tcacgtacta aacttgcaat aatgtctttc
    16141 gtacgatcaa tagcagcctg atcactagag aagatagata catttccttc ttcatcaata
    16201 tcaatcttaa cacctgtttc agcgatgatt ttatcgatag tttcgccacc tttaccgatg
    16261 acaactttaa tcttatcaac atcgattttt atcatatcaa tcttaggtgc cgttggtgct
    16321 aattgtggac gtggctctgc gatagctcca tgaaggacat caagaatttc aaagcgtgct
    16381 tttttagctt gcgcaagagc ttcttccaaa atttgagggg taataccttc aattttaata
    16441 tccatttgaa gtgctgtaat cccttcacgt gtacccgcaa ctttaaagtc catatcgcca
    16501 aaatggtctt ctaatccttg aatatcagtg aggactgtat agtttgttcc atctgatatg
    16561 agccccatgg caataccagc aactggagct tttataggca caccacctgc cataagagca
    16621 agcgttccag cacatataga agcctgagat gatgaaccgt ttgattctaa aacttctgca
    16681 actagtcgga tagcatacgg aaactcctcc aagcttggaa ggacttgttc taatgcacgc
    16741 tctcctaaag caccgtgacc aatttcacga cgccctgcag ctccataacg tcccgtctca
    16801 ccaactgaat attgtgggaa gttatagtgg tgcataaagc gtttcttata ctctggtgtt
    16861 agcccatcaa tgatttgtgc ttcacccata ggtgctaacg ttaatactga taatgcttgg
    16921 gtttgtcctc gtgtaaagag acctgatcca tgaacttgtg gtaaaaaatc aatctctgcg
    16981 tctaatggac gaatctcatc cacacgacgt ccatcaggac gaatcttatc ttctgtaatg
    17041 aggcgacgaa cttctgcatg ttccatttgt tctaaaattt cagcaacatc acgcataatg
    17101 cgatcatatt cttcatgttc agcgtaacgt gcctcatatt cacctataac tacttcttta
    17161 actgcttctg tagctgcttc tctagccttt ttctcttcaa cttgtactgc agcctgtaaa
    17221 gctatgttat gagtagcaat aatttctgct tgaagttctg gatcaacttg aagaagttct
    17281 acctcggctt tttctttacc aatagcagta acaatctctt cttgaaaggc gattaattca
    17341 cagacagcct catgtccttt aagaagagcc tctagcatga tttcttctga aagttctttc
    17401 gctccagatt caaccatgtt aatcgcttct ttagtacctg caaccgttaa ttctaacgca
    17461 gaagcctctt gttcttgtgc tgtcggatta ataataaaat taccatcaac ataagcaact
    17521 tgaactccag caattggccc attgaaagga atatcggaaa tcgataatgc taatgatgaa
    17581 ccaaacatag cagccattgg tgcgctagca ttttcatcaa atgaaagaac agtattgatg
    17641 acctgtactt cgttacgaaa tccctcagca aacatcggac ggattggtct atcaataaga
    17701 cgtgctgtta aggtagcatc cgtacttgga cgcccttcac gtttgttaaa gccacccgga
    17761 aatttcccag cagcatacat tttttcttcg taattgactt gaagtgggaa aaaatcacct
    17821 gttgacattt tcttagacat aactgctgct gtgagtacag tagaatctcc ataacgaacg
    17881 acaactgatc catttgcttg tttagccact tggccagttt caacaactaa tttttttcca
    17941 gcaaaaatca tttcaaagac ttgttttgac atattattct ccatattttt ttgtttacgc
    18001 gtttcctaca aagttcaatc tagctgttct aaactcaact ttgcataaaa ccacgtataa
    18061 gctttattct aacataattc tataaaattg agtagcgaaa atccgtggag gaagctgtat
    18121 tatcattcat taatgtaaac gtaaaaagtc tgattattaa tactagcaat cagaccttgt
    18181 tacaatgatt aaaataaatt tctagggtta atcttagctt gcttaataac cctcttcata
    18241 ggttaaaaca ctttcttact gctggcctcc tcaaagtact tacgttcata gtgcgtttaa
    18301 aacaaaaaac actagaaaat tttctagtgt ttttacttga ctctgttgtc tattaacgac
    18361 gaagtccaag tgattgaatc aattcacggt aacggttaac atctgtacga cgtaggtatg
    18421 ctaataagtt acgacggtga ccaatttttt tcatcaatcc acggtaagta gcgtggtctt
    18481 ttttgtgttg tttgatgtgg tcgttaaggt ggttgatttc ccaagtaaga actgctactt
    18541 gaacttcaac tgatcctgta tcaccttcgt gacgtgcata ttgagcaatg atttcatttt
    18601 ttttctcttt tgagattgcc ataatatatt ctccttttat tgcttcatcc gagtcttagg
    18661 attggcactc ctaaaaccaa gaaaaagttg gtttgtctta tagaccctct tattttatca
    18721 aattagaata aactttgtca aaagttttta atagaaatat ggataaccaa ctgtctctaa
    18781 aggaaaatcc ttcatagact aaacgagact ttgcatcttt ttgccgaaag accttagcat
    18841 aagtagatgt catactagtt ggcccacaca taaatattgt tggttgtcct tcaaaaacag
    18901 actgactaaa atcaagtctt cctttgaggc tactattatt taagtgaagt ttaaaattag
    18961 gattggcttt agcataggat tctaacatat cttgataaat caaattgtct tgatttgaaa
    19021 aggtataaaa gaagtcaact ctcttagtca aaatactatt ttctctaata aatgaaatga
    19081 agggagtgat gccgatacct cctgcaatcc aaacctgttc tttcttgtct ttatcaaata
    19141 acatatgacc ataagctcta tctaaggcta tttttgtccc aacttttagc tgtttgtaaa
    19201 tggatttcgt ataatctcct gaagctttaa cagttaaaaa tatcactcta tcatgaccac
    19261 ctgatatcga gaaaggatga gctgctgatt caaagccagc ttgataaatt tttaaaaaag
    19321 taaattgtcc atagtcatat cgataaggac gcttcattgc tatttcaatc tcagttgtat
    19381 catggttaag atgagtaacc ttttgaacgt accctacacg acgaaaacgc attctagaat
    19441 ataggaaaat aatataaaat ccagatataa ccccaatgac agcataacct aatacgatta
    19501 aactgagtaa agtatttcct agtatcctat ctcctaaaat cataaaagta tgtaccaatc
    19561 ccaaaatata agctaaataa acaaagcgat gaataaaacg ccaaatttca tactttaaaa
    19621 aatttccgaa ataagctaca aaaacaatac ttagaaaaag atataatccc gcactgccaa
    19681 tagtctttgc aaactcactt ccatgtccgc cttgtccaag accaattttg tgcaatagaa
    19741 gcaaaatcat tgaaaataca gccattacct tgtggtaaaa atacatattt tcaatacctt
    19801 gaaagtaact ttcaagtatc ctaaatttgg ttgataagag gaatgttagc gataaacttg
    19861 tcaaagctaa tcctggtaca acaaattcac ttaatcctgc attttgccat gctactagtg
    19921 tcaccactat gctagataaa attaaaacaa tacctttcca agctcgcata acgaccaccc
    19981 ctttattttt agtaaggata aaataccaca agatacttat acaaaactta aaaatttcag
    20041 aaaataacaa aaaggcaact ccaatgttgc ctttttgtta tgatttataa attgatttgc
    20101 aatgctgtac tatatcttta acagcgagtc catactcctc taataaatat tccatctgac
    20161 ctacctgacc aaatcgctca tcaataccca tacgactaac agtagtgtct ttctccatac
    20221 taagccattc gcataatgca cttccgatac cgccaatacg gttatgattc tcaatagtta
    20281 caatggattg atctatcagt aatggtttca gttcttctgg aaggggctta attttaaaaa
    20341 ggtcgataac tgaggcctca atccctagtt ctttgaggta gtcggctgct tctatcgctc
    20401 tagaaaccat aataccactt gctacaagag taatatcttt tccctggcgt aattgtataa
    20461 aaccctttga aaaatcttcg cgtccctcat aaactgcagt gggggcctta cgaatagttc
    20521 taatgtattt taggccatca atacttaatg tttgttttaa aattgcttca aactgaatat
    20581 catcactaac ctcaaaaata gtcgctttgg gaattaacct tagtagacct aactcttcaa
    20641 aaggcatatg agttccgcca ttcatttctg cgctaattcc tgcatctgag ccaataatcg
    20701 tagctgataa ttgtgagtaa cctaacgata agaatacttg atcaaagaca cgccttgacg
    20761 cgaaaggacc aaaagtatga aggtatggct tgtaaccttt aatggcgagc cctgctgcaa
    20821 ggcctaccat ctcagcctcc ataataccta gattaatata acgttttcca aattctgatg
    20881 ccagtgcatt tgttgacatt gaactcgaca aatcggcttc taaaacggta atttgtttat
    20941 tttcttgatt agcttgaagg aggaaatcac gatagacaag cctcatttct ttggttgaac
    21001 atctcattct accacctcca aactttctct caagcttatc aacgctcgct ctaacattgt
    21061 tttttgttgt aaatctggtc gtaaatgatg attagaagct aactcctcca actctttcac
    21121 accttgtccc ttgatgctat ctaaaacaat acattttggt ctgacactat ttgaatcttg
    21181 aaaagtttta atagctttgt caattgcctc aatgtcatct ccttttacac gtactgcatc
    21241 aaatccaaaa gcttcaaatt tagcaacaaa gtctccagga ttacaaatat cagctgtcaa
    21301 accatctaat tgttttttat tatcatctac aaaaacaatt aaatggtgca gttgatgatg
    21361 cgcagcaaat tgtatagcct cccaacattg tccttcattt aattcaccat cacctacaat
    21421 agtataagta taatagcttg aattctcaat cttttgagca taagcaattc ccgttgcaat
    21481 actaatgcct tgacctaacg agcctgtcgt tacatctata ccaggagtta aattgcggtc
    21541 aggatgcgaa ggtagtttgg taccgtttgt attgagcgaa tgaaggaatg ttttatcaaa
    21601 aaaacctttt aaatagagtg tactatacaa ggctggacca gcatgtcctt ttgataagac
    21661 catataatcc cgatcacttt ctttgaattt ttcaggattt atatccataa tatcaccata
    21721 cagaacagcc aaagcctcaa caattgataa actaccccca tagtgaccga atcctaaatg
    21781 gtttagcgtt tctagagtat ttaaacgaat ctccgttgca aatcgtctta gagatgaata
    21841 tctttcttct ggtgatgtca ttattaacct tccttatggg ttgattttcc aacaaatgaa
    21901 atgccaaaaa gtccaattaa gataactaca ataccaataa caatagctat cgcaccgccg
    21961 acatgattaa gagcgccaag aatgataccg gtcaaaccaa aatcagcatc tgagaatgtt
    22021 gaccctttga aaccaagtcc acctagtaca ggcatcaaga aaatcggcaa gaaactgatg
    22081 aggatacctt gaacaaatgc accaatagtc gctcctctca caccaccaga agcattaccg
    22141 ataacacctg ccgttgcccc acagaagaaa tgaggaacga cccctggtaa aatgacagta
    22201 gtgccagtca caatcatgac tatcatacta accaatccac caacaaaact tgagataaaa
    22261 ccaatcaata ctgcattagg tgcataaggg taaacaattg gacaatccaa ggctggtttt
    22321 gaatttggaa ccaatttttc tgagattccc ttaaaggctg gtacaatttc acctaggata
    22381 aggcgtacac ctgctaaaat aacgaataca cctgccgcaa attgcccagc tagttggagg
    22441 gcataaacaa gaccattgac accgttactt atttcttttg caatataagc ctcaccagca
    22501 aacactgcaa cgataaggta aataattgcc attgagatag taatacttac ggtactgtca
    22561 cgtaaaaatg ataaactctt tggaaattta atatcttctg ttgattttga tttatctcca
    22621 acaattccac caataaatcc actcaaccaa tagcctaaag aaccaaagtg cccaagtgcc
    22681 accttatcat tacctgtcag ttgaatcatg tatttttgca caaatgcagg agatacgctc
    22741 ataataatcc ctaacgctag acctccaaaa aggatgagcg agaacgaagt aaagcctgct
    22801 acggcaaaaa taacagcaat catacaagcc atatacaatg tatgatgtcc tgtcagaaaa
    22861 atatatttaa attttgtaaa acgagcaatt aaaatattaa aaatcattcc cgctaacata
    22921 atcaaagcag ttgctgagcc atacttcgta agagctactg ctacaatggc ttcattatta
    22981 ggaactaccc ccaccaaatg aaaagcatgt tcaaacattt ttccaaatgg attcaaggaa
    23041 ttttggacta tccctgtacc tccagaaacc actaagaagc caacaaatgt ttttattcca
    23101 ccttttacaa tatcaggaac gccttttttc tgcagtacta aaccgataat ggctatcaag
    23161 gcgactaaaa tagctggcgt gctggcaata ttaactaaaa aatccaatag acctttcatg
    23221 gcatcaacct cctagattaa gcctttttct tggcaaatac ctgtcactaa ttctctcaat
    23281 tcatccatat caatgatact gtttaaaata cgaacatccc ccaggtgacc agctgaatcc
    23341 tccaaatcac gaccaacaat ccaaacatct gctgcacttg gatctgctcc acctaaatca
    23401 taatgttcaa cttcaacatc agaaaccccc aaatccttca aaatagactc aatattcatt
    23461 tgtaccataa aacttgaacc taaacctgat ccacatgctg ttccaattct taacatcttt
    23521 aatcctcctg ttttataatc atttcaatat cttgatacct acgtgaagct aacaaagctt
    23581 taacattatt attatcacga agtattgttg tcaaatgcga tagagctttc aaatgcgttt
    23641 cattatcaat agctgctatg cataataaga gataaatttc ctgtttgggg tcatccaata
    23701 aataaatagg ctgttccaaa acaagcattg acataccgac agagtttacc ccatcttctg
    23761 gtctggcatg tggaatagca atacctttac caagattgat aaaaggacca aactcttcaa
    23821 ctttttcaat catagcttct gggtaacttt ccgtaatttt tccagaagct aacaagggtt
    23881 ttgccgctaa acgaattgcc tctttccaat tcatttgttc agaacttgtc tgataagttt
    23941 cctctgttat aagttggtgc aacaagggcc tcacatcctt tctaagtata tcttgtttta
    24001 ccattgtcct caaagccaat tttaattcct cttcacaatg aacatgagcg tattttttta
    24061 tagttgctat taattgatca agttggaaat cgtcaaggca agccttggga aaatctgaaa
    24121 taaccagttc tttgagctgt tgtacttgct ctgctgtcat catcagcgaa actagataat
    24181 taggtttttt tgtttctaca aatatagtag aaaagaccat atcgtaagtt tgattgtcaa
    24241 tcagtttcag ctgctcaatt ttagagacac gatgaaaatg aatttgagga aataatccac
    24301 gcaatttttc cttaatcact aaggatgaac taacgccgtt tggacagagt atcaaagctt
    24361 tataagacat tgattgtgta ccacctgatt gtctaagata gcctccaaag tgaatcacga
    24421 agtaagaaat ttcactatcg ggtattaatc ctacttgctc ttccaaagga cgtagtgcct
    24481 ttttaaccaa tagaaataaa tcactgtaat gctctttaat attttgtgta taaccactat
    24541 cgccagttaa accatatttc aatcggaaat aagctggaat aatatgacgc ttcagacctt
    24601 gtaataactc ttctttattg gaaaaattaa gaagtgaaac agtttccatc tcatcaacga
    24661 ttgcttttgc caatgcttca aagaaatcat catctttagt tccctcacct tcaaaacagc
    24721 cagataagat caaggtaata taatcttttt ctttctgatc taatggttta gataaggaaa
    24781 tatcctgact taaattagtc acaataatag tcgttaactc ttttaattgt tcagaaacaa
    24841 taggagaacc ctgcaaaaca cgatctactg acctttgata acgacacagt attaaaatga
    24901 taaaatataa agatttctca agcctatctt gtattggaga aagttgaaaa gattcataga
    24961 aatattctac cgtcttttct aacttctcat atgagagagc gaatttccaa gaagacaaga
    25021 tataattcaa agaccaaaaa ccaatgggag attctagtag ttgagttatc atctgaaaag
    25081 caacttgatg cttatccatc tcatctccta ctagactata tccctttgct cttgtataag
    25141 tgagtgagat acctcttttt gctaatttag aacgtagcat cttgatatct gataaagtag
    25201 tattcttact tactttcaaa aaatcttgat aatgcacatt ggagacaaac tctcttcggc
    25261 aaaagctata aaggtaaatc agacataccc tttcttcctg ggttaggtaa aattgctcag
    25321 cttctaataa ggagacaatt ttatcatgct ctttttcatc tcctaataac tgataacgtc
    25381 cagcagagtg agcaatagct ggtaggtgat tttttgctaa aaaactattg agatagttaa
    25441 tttgctctat gataactttt attgaaaact ttgtttgcac tgttaactca tacaaggaaa
    25501 gatttttccg agctaaaaat accaacatta tcacaacagt cttattatca accat
//
LOCUS       DATRSD010000022        25484 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRSD010000022 DATRSD010000000
VERSION     DATRSD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25484
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.022"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..402
                     /locus_tag="VBD85_000723"
     CDS             <1..402
                     /locus_tag="VBD85_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133289.1"
                     /translation="SRLWTNLFVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALA
                     LAFVFNYLLLFQLLALGHHFDYQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDSIL
                     IRELKPVILLIVLMLIVTEYYIPYKIKKMID"
     gene            460..1260
                     /locus_tag="VBD85_000724"
     CDS             460..1260
                     /locus_tag="VBD85_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO7133290.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS"
     gene            1250..1885
                     /gene="trmB"
                     /locus_tag="VBD85_000725"
     CDS             1250..1885
                     /gene="trmB"
                     /locus_tag="VBD85_000725"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO7133291.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            1929..2016
                     /locus_tag="VBD85_000726"
     tRNA            1929..2016
                     /locus_tag="VBD85_000726"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1963..1965,aa:Ser,seq:gga)
     gene            2375..2851
                     /gene="rimP"
                     /locus_tag="VBD85_000727"
     CDS             2375..2851
                     /gene="rimP"
                     /locus_tag="VBD85_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO7133292.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            2878..4038
                     /gene="nusA"
                     /locus_tag="VBD85_000728"
     CDS             2878..4038
                     /gene="nusA"
                     /locus_tag="VBD85_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO7133293.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            4060..4356
                     /locus_tag="VBD85_000729"
     CDS             4060..4356
                     /locus_tag="VBD85_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO7133294.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            4349..4651
                     /locus_tag="VBD85_000730"
     CDS             4349..4651
                     /locus_tag="VBD85_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO7133295.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            4671..7424
                     /gene="infB"
                     /locus_tag="VBD85_000731"
     CDS             4671..7424
                     /gene="infB"
                     /locus_tag="VBD85_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO7133296.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADHKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQELAK
                     AAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQTRSDKSRQFSN
                     ENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVTERKFHELPKE
                     FEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAHA
                     KVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEA
                     GGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIVAADDGVMPQT
                     VEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGESEFVEISAKF
                     GKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQD
                     PIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYADEKAARAA
                     GEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSVEALAASLLKI
                     DVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQADADDVEIRQHS
                     IIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSS
                     VRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDDTIEAYIMEEI
                     KRK"
     gene            7515..7883
                     /gene="rbfA"
                     /locus_tag="VBD85_000732"
     CDS             7515..7883
                     /gene="rbfA"
                     /locus_tag="VBD85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO7133297.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(7967..8971)
                     /locus_tag="VBD85_000733"
     CDS             complement(7967..8971)
                     /locus_tag="VBD85_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133298.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            9135..9551
                     /locus_tag="VBD85_000734"
     CDS             9135..9551
                     /locus_tag="VBD85_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO7133299.1"
                     /translation="MTISSAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSPLIDEDLAMMSEVDSVFQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQSSLEAKKGKTLERVACNCIPGQCQCH"
     gene            9564..11798
                     /locus_tag="VBD85_000735"
     CDS             9564..11798
                     /locus_tag="VBD85_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO7133300.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSESGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGMIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEA
                     AQTSKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGGDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSATI
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            11839..12045
                     /locus_tag="VBD85_000736"
     CDS             11839..12045
                     /locus_tag="VBD85_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO7133301.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVIGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            12155..12769
                     /locus_tag="VBD85_000737"
     CDS             12155..12769
                     /locus_tag="VBD85_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO7133302.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDILGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            12784..13596
                     /locus_tag="VBD85_000738"
     CDS             12784..13596
                     /locus_tag="VBD85_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7133303.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSTSLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNKAGVAKAIYDYALR"
     gene            13709..16351
                     /gene="polA"
                     /locus_tag="VBD85_000739"
     CDS             13709..16351
                     /gene="polA"
                     /locus_tag="VBD85_000739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO7133304.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASNEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKTEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSNVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIRELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            16381..16821
                     /locus_tag="VBD85_000740"
     CDS             16381..16821
                     /locus_tag="VBD85_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO7133305.1"
                     /translation="MVYHFQNPSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVECQKLHSETN"
     gene            16903..17382
                     /gene="perR"
                     /locus_tag="VBD85_000741"
     CDS             16903..17382
                     /gene="perR"
                     /locus_tag="VBD85_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO7133306.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            17535..19100
                     /locus_tag="VBD85_000742"
     CDS             17535..19100
                     /locus_tag="VBD85_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO7133307.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKSSEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            19213..19899
                     /locus_tag="VBD85_000743"
     CDS             19213..19899
                     /locus_tag="VBD85_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133308.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            19901..20938
                     /locus_tag="VBD85_000744"
     CDS             19901..20938
                     /locus_tag="VBD85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7133309.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRCFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAESHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRLYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(20952..21692)
                     /locus_tag="VBD85_000745"
     CDS             complement(20952..21692)
                     /locus_tag="VBD85_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO7133310.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISYYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWQFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            21879..23021
                     /gene="tgt"
                     /locus_tag="VBD85_000746"
     CDS             21879..23021
                     /gene="tgt"
                     /locus_tag="VBD85_000746"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO7133311.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGMNNRNF"
     gene            23128..23439
                     /locus_tag="VBD85_000747"
     CDS             23128..23439
                     /locus_tag="VBD85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133312.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDETFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            23446..23985
                     /locus_tag="VBD85_000748"
     CDS             23446..23985
                     /locus_tag="VBD85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7133313.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYTNIPLDKSLLQNLVFIPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            24124..24900
                     /locus_tag="VBD85_000749"
     CDS             24124..24900
                     /locus_tag="VBD85_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7133314.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            24900..25406
                     /locus_tag="VBD85_000750"
     CDS             24900..25406
                     /locus_tag="VBD85_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO7133315.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
BASE COUNT         8612 a         3903 c         5172 g         7797 t
ORIGIN      
        1 tcgcgtttat ggactaactt atttgtgagg gcatttctaa gaagctcaga ttatcttggt
       61 ttaacaataa ggttggtaac tttaaatatc ttatcagtca tctttgttaa cgaaacctac
      121 ctcgcccttg ctctggcatt tgtatttaac tacttattat tgttccaact attagcttta
      181 ggtcaccatt ttgattatca atacatgaat cagctctatc cagttagatt aaatgctaaa
      241 gcaagtcagc taaaaggttt tctcagagta ttatcatatg ctgtgacagt aattgatagc
      301 atacttataa gagaattgaa acctgttata ttattgatag ttttgatgtt aattgtgacg
      361 gaatattaca tcccttacaa aataaagaag atgattgact aaagcctata aaacaagtaa
      421 aataagagga aacgactaaa tgaaaaggag ggactgctgg tgacaatatc aaatcaagaa
      481 ttgacgctta ctcccttaag aggaaaaagt ggcaaagcct atattggtac ttatccaaat
      541 ggtgaacgtg tctttgtcaa atataatacg acacctattt tgccagcctt agctaaggaa
      601 cagattgcgc cacaactact gtgggcacgc cgtacaagta atggcgatat gatgagtgct
      661 caagaatggt tagatggtcg aacattgact aaagaagata tgggtagtaa acagattatt
      721 catattttat tgcgcctgca caagtctcgt cccttagtaa atcagctgtt acaattaggg
      781 tataaaattg aaaaccctta tgatttgttg atggattggg aaaaacaaac tcctattcaa
      841 atccgggaaa acacttattt gcaatctatt gtgactgagt taaagcgtag tttacctgag
      901 tttcgaacag aggttgcaac aatcgttcac ggagatatca aacatagcaa ctgggttatt
      961 acaacaagtg gtttaattta tttggttgat tgggattctg ttcgtttaac agatcgcatg
     1021 tatgacgttg catatatttt aagtcattat attccacaaa aacactggaa agattggctg
     1081 tcttactacg gttataaaga taatgagaaa gtctggagca aaattatctg gtatgggcaa
     1141 ttttcatatt tatcacagat aatcaaatgt tttgataaac gagatatgga gcatgtcaat
     1201 caagaaattt atgaattacg aaagtttaga gagttaatta aaaagcataa tgcgagttag
     1261 aaaacgtaag ggtgcagagg aacacttaga aaataatcct cactatgtta tttcaaatcc
     1321 agaagaagcc aaagggcgtt ggcatgagat ttttggaaat aataacccca ttcacataga
     1381 agttggttcc ggaaaagggg cttttatcac tggtatggca gagcaaaatc cagatatcaa
     1441 ttatattgga attgatattc aattgtcagt gctttcctat gctttggata aagttctaga
     1501 tagcggtgct aagaatatca aattactatt agttgatggt tctagtctat caaactactt
     1561 tgatactggc gaagttgatt tgatgtatct taactttagt gatccatggc ctaagaaaaa
     1621 gcatgagaaa cgtcgattga cctataagac tttcttggat acctataaag acatactgcc
     1681 tgagcagggg gaaattcatt ttaaaacaga taatagaggt ttatttgaat atagccttgc
     1741 tagcttttct caatacggta tgactttaaa acaagtttgg ttagatttac atgcaagtga
     1801 ttaccagcaa aatatcatga ctgagtacga acgcaaattt tcaaataaag gacaggttat
     1861 ctatcgtgta gaagcacgat tttagaataa gtaaatagta agtgggtagt tatttgctac
     1921 tcctttatgg agaggtcgca tagtggctga gtgcgcacgc ttggaaagcg tgtagtccct
     1981 aatgggggct cgggggttcg aatcccctcc tctcctttaa tcttgttaat gtatcagtgt
     2041 ttattaagtg ttcacctttt tcactcgtca aaagtccaag tttcgaaaaa tttcttttat
     2101 tttatcatcg tttctctttg agcttgaagt attagcataa agttttctaa gctcttacaa
     2161 agaactgagg acaaaatttt aattttttga tataagatta gtgtacggtg atgctaattt
     2221 aaagtaaatg attgcaataa tcgtattttg tgctataata gtattaaata atacagaaag
     2281 gggcggagtt ttacttcgcc tcttatgtgt gtgattcata gaatttatag tcattataag
     2341 ttctaaagca agttagaaag aagaggtagc tgttatcgca aatcaaacta ttgttgatat
     2401 tgttactcaa gtcgtaactc ctgctattca agcaccgttt gaattagtag atgtagaata
     2461 cgaaaaaatg ggtggcgact atgtgctcag tattttaatt gataaacccg gaggcattac
     2521 ggttgaagat acagcacaat tgacagacgt tgtaagtccc ttacttgaca caattcaacc
     2581 agatccattt ccagaacagt atatgcttga agtctcaagt cctgggttgg aaagaccact
     2641 taaaacagca gaagcattat caaatgctgt tgggtcttat atcaatgtaa gtctgtataa
     2701 atccattgat aaagttaaaa tatttgaagg tgacttatta tcatttgatg gtgaaacctt
     2761 gacaattgat tatatggaca aaacacgtca taaaactgta gatattccct atcagacggt
     2821 tgcaaaagcc cgtcttgcgg ttaaacttta agaaaggatg attcttgcga agcaagaatg
     2881 gacactatga gcaaagaaat gctagaagcc ttccgtattt tggaagaaga aaaacacatt
     2941 aataaagaag atattattga tgcagttact gagtcactaa aatctgccta taagcgtcgt
     3001 tatggtcaat cagaatcatg cgttattgaa tttaacgaga agaaagcaga ctttaccgtc
     3061 tatactgttc gtgaagttgt tgatgaagtt tttgatagcc gtttggaaat tagcctaaaa
     3121 gatgcccttg ctattagctc agcgtatgaa cttggtgata agattcgctt tgaggaatct
     3181 gttacagaat ttggacgcgt ggcagctcag tcagcaaaac aaactattat ggaaaaaatg
     3241 cgtcgtcaaa tgcgtgaagt aaccttcaat gaatacaaac aacacgaagg tgaaattatg
     3301 actggtactg ttgaacgatt tgaccaacgt tttatttatg tcaatctcgg ttcacttgag
     3361 gcgcaattat cgcatcaaga tcagattcct ggagagagct ttaaatctca tgatatgatt
     3421 gatgtttatg tctataaagt ggagaataat cctaaaggag tcaacgtttt tgttagccgt
     3481 agtcatccag agtttatcaa acgcattatg gagcgagaaa ttccagaagt atttgatgga
     3541 actgttgaga ttatgagcgt ctctcgtgag gctggagatc gtactaaagt tgctgtacgc
     3601 agtcataatt caaacgttga tgcgattgga acaattgttg gacgcggtgg aagtaacatt
     3661 aaaaaagtta tcagtaattt ccatcctaaa cgcgttgatg ctaaaacagg attggagatc
     3721 ccggttgaag aaaacattga tgttattcaa tgggttgaag atccagcgga attcatttac
     3781 aatgctattg caccagccga agttgatatg gtactttttg atgatgaaga tacaaaacgt
     3841 gctactgtag ttgttccaga tagcaagtta tcgctagcta ttggtcgccg aggacaaaat
     3901 gtgcgtttag cagctcattt gactggctat cgtattgata ttaaatctgc aagtgaatac
     3961 gaaaaaatgg aagcgcaaga acttcaaact gaggaagtag ctcaagaatc tgaagttatt
     4021 tcagataagg cagaataata taaatgagag aggtgtttta tggctaaaac aaaaaaaata
     4081 cctttaagaa aatcagttgt ttctggtgaa gtgatcgata agcgagatct tttacgaatt
     4141 gttaaaaata aagaaggaca ggtttttata gatcctaccg gaaagcaaaa tggtcgaggt
     4201 gcctacataa aattggacaa tgatgaagct atcctagcga aaaagaaacg agtgtttgat
     4261 cgcagttttt cgatggaagt ttctgatgaa ttctatgatg aactattggc atatgtcgac
     4321 cataaggtca aaagaagaga gttaggcctt gaataattca gaaaaagtat taaatctcat
     4381 tgggttagca cagcgtgcgg ggcgccttat tacaggtgaa gaattggtta ttaaagcaat
     4441 tcaaaatcaa caagtatcgc taatctttct agcaaatgat gctggcccaa atttgacgaa
     4501 aaaagtaact gataaaagta attactacaa aacagaagtc tccacagtgt tttcaacact
     4561 ggaattaagt gatgcgttag gcaaaccaag aaaagtggtt gctgtcgcag acgctggatt
     4621 ttcaaagaaa atgaggactc ttatggcata acagaatagg aggacatcaa ttgtcaaaga
     4681 aaagattaca cgaaatcgct aaggaaattg gaaaaacgag taaggaagtg gttgagcaag
     4741 ctcaatcgct tggcttacct gtaaaaagtc atgcttctag cgttgaagaa aatgatgcta
     4801 cacgtattgt tgagagtttt agctcttcta aaactaaagc tccaacaaat tctgttcaaa
     4861 ctaatcaagg tgtgaaaaca gaatctaaaa ctgttgaaac caaacaaggc ttgtccgatg
     4921 ataaaccttc aactcaacct gtagctaaac cgaaaccaca aagtcgtaac tttaaggcgg
     4981 agcgcgaggc tcgtgctaaa gctgaagcgg agaagcgtca acataatggg gatcaccgta
     5041 agaataatcg ccataatgat acacgctctg acgacagacg ccatcaaggt caaaagcgtt
     5101 caaatggtaa tcgtaatgat aaccgacaag gtcagcaaaa taaccgtaat aaaaatgatg
     5161 ggcgttatgc tgatcataaa cagaaacccc aaacgcgtcc tcagcaacct gcaggcaatc
     5221 gcattgactt taaagctcga gcagcagctt tgaaagctga gcaaaatgct gaatactcac
     5281 gtcatagtga gcagcgtttc cgtgaagagc aagaactagc taaagcagca gcgttaaaag
     5341 cgcaagaaga agctcaaaaa gctaaggaaa aacttgcttc taaacctgtt gctaaggtaa
     5401 aagaaattgt caataaagta gccgctacgc catcacagac agcagatagt cgtcgtaaaa
     5461 aacaaactcg ttctgataag tctcgtcaat tttcaaatga aaatgaagat ggacaaaagc
     5521 aaacaaaaaa taagaaaaac tggaataatc aaaaccaagt gagaaatcaa agaaatagta
     5581 actggaatca taataagaaa aataaaaaag gtaagactaa tggagctcca aaaccagtaa
     5641 cagagcgtaa attccatgaa ttaccaaaag aatttgaata tactgagggc atgaccgttg
     5701 cggaaattgc aaaacgtatt aaacgcgaac cagcggaaat tgttaaaaaa ctctttatga
     5761 tgggagttat ggcaacgcag aatcaatcat tagatggaga cactattgag ctattgatgg
     5821 ttgactacgg gattgaagca catgctaagg ttgaagttga tgaagctgat attgaacgct
     5881 tctttgcaga tgaggattac cttaatcctg ataacttaac ggaacgtccg cctgttgtta
     5941 ctatcatggg acacgttgat catggtaaaa caactcttct tgatactcta cgtaattcac
     6001 gtgtcgctac tggcgaagct ggtggaatta ctcaacatat tggtgcttat cagattgaag
     6061 aagctggcaa gaaaattact ttcttggata caccagggca tgcggccttt acttctatgc
     6121 gtgcgcgtgg tgcatcagta actgatatta ctatccttat cgttgccgct gatgatggtg
     6181 ttatgccgca gacagttgag gcaattaatc actctaaagc tgcgggtgtc ccaattattg
     6241 ttgctatcaa caaaattgat aaacctggtg caaatccaga acgcgttatc agtgaattag
     6301 ctgagcatgg tgtcatctct actgcatggg gtggtgagtc tgaatttgtt gaaatttcag
     6361 ctaagtttgg taaaaatatt caagaattac ttgagacagt tcttttagtt gctgaaatgg
     6421 aagaattaaa agcagatgca gatgttcgtg ctattggtac tgttattgaa gcccgtcttg
     6481 ataaaggtaa gggagccgtt gcaacgcttc ttgtccaaca aggtacttta aatgtacaag
     6541 atccaatcgt tgttggtaat acatttggac gtgttcgtgc gatgactaat gatcttgggc
     6601 gtcgtgtcaa agttgcgggt ccatctacac cagtttctat tactggtttg aacgaagctc
     6661 caatggctgg tgatcatttt gctgtttatg cagatgagaa agctgcgcgt gctgcaggtg
     6721 aagaacgtgc aaaacgtgct ctcttaaaac aacgccaaaa cacgcaacgt gttagcctcg
     6781 agaacttatt tgataccctt aaagctggtg aagttaagtc agttaatgtt attatcaaag
     6841 ctgatgtgca aggttctgta gaagcacttg cagcttctct ccttaagatt gatgttgaag
     6901 gagtaaaagt taacgttgtt cactcagcag ttggtgctat taatgagtct gatgtaacac
     6961 ttgctgaagc atcaaatgct gttattatag gatttaacgt acgccctact ccacaagctc
     7021 gtcaacaagc agatgctgat gatgtcgaaa ttcgtcaaca tagcattatc tataaagtta
     7081 tcgaagaagt tgaagaagcg atgaaaggta aacttgatcc tgaataccaa gaaaaaatcc
     7141 ttggtgaagc cattattcgt gagaccttta aagtttcaaa agtgggaact attggtggat
     7201 ttatggttat caatggtaaa gttactcgtg attcaagtgt tcgtgttatc cgtgatggtg
     7261 ttgtcatttt tgatggtaaa ttagcaagtc ttaaacacta taaagatgat gttaaagaag
     7321 tcggcaatgc tcaagaaggt ggtctaatga ttgagaatta taacgatctc aaagaagatg
     7381 acactattga agcatacatt atggaagaaa ttaaacgtaa ataaccccta tgagaaactc
     7441 aacttgtatc tttagttggt agggaaatac aaaattatgg ctaggctcgt cctagctttc
     7501 aagttatgag cacaatgaag aaaggattaa ttatggctaa tcatcgtatt gatcgcgttg
     7561 gaatggaaat taaacgtgaa gtcaatgaga ttttgcgtct ccgagttaat gatccacgtg
     7621 tacaagatgt cacaattact gatgttcaga tgttgggtga cctttcaatg gctaaagtat
     7681 tttatactat tcattcgaca cttgcttctg ataatcaaaa agcacaaatt ggtttagaaa
     7741 aagcaactgg tactattaag cgtgaacttg gcaaaaacct tactatgtat aaaataccag
     7801 atttacaatt tgttaaagat gaatctattg aatacggaaa taagattgat gaaatgttgc
     7861 gcaatctaga taaaaaagat tagaatcaag attaagaata ataaaaaata aaccatgtca
     7921 aaatcatggt ttatttttta tagctttaga gttgagatag ctagtattaa ggagttttat
     7981 tgatgatatc aatcatttga tactgtgctg ttttagcttc ttcaataggg taaattggat
     8041 aaacatgatt cattccctct tgtgcaatat agttaacggg gtgattagct gcttggagtt
     8101 gtgcagcata atcgcgaata tctgggaaga atatttctcg tgttccagta aataacgtaa
     8161 taggagcgag tttagtagca ggggcatttt taggactaac atatgtatgg ttagtattat
     8221 tagaaccatt tgcccaaatt tcacctacac gtgcgagacc ccaagcagat aggataggat
     8281 cagtatcttc gtatttggga atttctggat gtttcattgt cacatctagc catggagata
     8341 agaggataat atttttaggt tgaggaatag cctcttgccc tgattggtga gacaatgcat
     8401 gcgctaatcc tagtgctaat cctccgccag ctgaatcacc catcaaagtg agattagcgt
     8461 ttttttcatg aaagtgtcta taaaggttaa ccaatctagg gatagcataa tcataggtat
     8521 aacgaggagt ttttggataa attggtaaaa taatttttgc atcaagacta gttgagagcg
     8581 tcttaagcat gctgatatgg taaggcgttg gattatttaa gtaagagcca ccagctaaat
     8641 agaaaatggt cttttgatta gctttaccgt tgtcattcca agaatagact tgcatatcaa
     8701 aaagtctctc ttgaacaagc cctccattaa cagagtttgg gtctaagtaa taacccttat
     8761 cagattgttc acggctagca tttaagtact tttgaaatga gctttcatct tgaactttat
     8821 aataactttt atcgcgcttt agccaaaagt attgttcgat aaaccaactt ttccatgaac
     8881 gcccacttgc ttttgcatgt ggtgtacagg cgatggataa aatggtgata atactaaata
     8941 gtagcaatag ttttttgaag gacggtttca aggtattctc cttaatataa tgttattttt
     9001 tgtgtaatat tattataata aaaccaacat ctttgtcaat agaataaaga aagatgagaa
     9061 atgtttttcg gtttacaaat gtagatttat ttgctaaaat tggtttacaa acgtaaatcg
     9121 aaagaggtga gaaaatgact atttcaagcg cagaatggga aattatgaga gtggtttggg
     9181 cacagcagaa tacaaccagt aatgagatac tagcagtttt actagagaag tatgattgga
     9241 ctccttcaac tgtcaaaacc ttattaagaa ggctcttaga taaaggctac gtgagcagag
     9301 aaaaaatggg aaaaggattt agctattctc ctttaattga tgaagattta gctatgatgt
     9361 cagaggtaga tagtgtattt caaaaagttt gccagaccaa acatgttgct attgttagac
     9421 acttattgga aagtatacca atgacagaga aggatcgatt aaatttgcaa tcatcactag
     9481 aagctaagaa aggcaaaaca cttgaacgtg tagcttgtaa ttgcattcct ggtcagtgtc
     9541 aatgccatta gaaaggagaa aatatggcta atagaaaaga aacatttttg attgatggga
     9601 tgacttgtgc atcttgtgct ttaacgatag aaaaagctgt caacaagtta gaccatgtgg
     9661 atagtgcagt agtaaattta gcaacagaga aaatgacagt tacgtttgat gatacaacct
     9721 tatcgccaaa tgtgattgaa gagtgtgttt ctgagagtgg atacgaagca tcgcttttca
     9781 aagaggaaac ttctaaaagc caatctgaac ggcaccaatt agccattgaa aagatgtggc
     9841 atcgcttttg gatgtcagca gttgcaacta tacctcttct ctatatttcc atgggaccaa
     9901 tgattaactt atggttacca tcatttttga tgccagataa gggacctcta aactatggta
     9961 tgatacaatt attgttaaca ctaccggtga tgtattttgg gcgtatcttt taccaaaatg
    10021 gttttaaagc actttttaaa aggcatccca atatggattc attagttgct atagccacaa
    10081 cagcggcttt catttatagc ttatatggat tgtacgaaat attacagggt gacatacact
    10141 acgctcacca gctttatttc gaatctgttg cagttatttt aacacttatt actctaggaa
    10201 aatattttga aatattatcc aaagggcgta cttcggcctc gattgaaaaa ttattaactt
    10261 tatcagcaaa agaagcaaga gttatcaaag atggtgaaga ctatatggtt cctttggata
    10321 aagttaaaat aggtgaaaca attcttgtca aacctggaga aaaaattcct cttgatggcc
    10381 atgttgtagc aggagaatca agtattgatg aatccatgtt aacaggtgaa agtattccag
    10441 ttgaaaaaaa ggttggtagt aaggtttacg gtgccagcat taatgggcaa ggtagcttaa
    10501 cgattttcgt tgaaaaagaa gctggcggtt cccttctatc acaaattatt aacttagtag
    10561 aagctgcaca gacaagtaag gctccaattg ctaacttagc agacaaagta tcaggagtct
    10621 ttgtcccttt tgttattgtc attgcaattt tatcaggctt gagttggtac ttaattctgg
    10681 ggcaaagttt tgccttctct ctgaagatta tgattgcagt cttagtgatt gcttgtccat
    10741 gtgctttagg gttggcaact cctacagcta ttatggtggc tagtggtaaa gcagctgaga
    10801 atggtatttt atttaaaggt ggtgaagtgt tggaaaaggc tcaccacatt gatacaatag
    10861 tttttgataa aacaggaacc ttgaccaaag ggaaaccaga agtagttgct atcaaaacat
    10921 atgggggaga taaagaggag tttttaggtc aggtagcatc agtagaaaaa ctttcaaatc
    10981 atccacttag ccaaacaatt gttaataaag caaaagagaa agaacttccc ttaagggaag
    11041 tcatggcttt taaaaatatt ttgggttacg gtttgagtgc aactattaat ggtaaaacta
    11101 tgcttgtagg taatgctaac ttaatgacta agaacgatgt taatttagat ttagcaaagg
    11161 ctgatataga aatagctcaa gaagaagcac aaactgtggt ttacgtttcc gaaaatggag
    11221 tgttgagcgg tttaataact ttaacggacc agctaaagac agatagccaa gaaacagtta
    11281 agcagttaca aagattagga tttaatttag ttttacttac tggtgataat aaggctagtg
    11341 cagatgccat agcacaaaaa ttgggtatca cgacagttgt ttcagaagtt ttacctgatc
    11401 agaaagcaaa cgttatttta gaacttaaag aaaaaggcgg acagattgcg atggttggtg
    11461 atggcatcaa tgatgctcct gcgctagcta gcagtgatgt aggtatctct atgagttctg
    11521 gaacagatat tgctattgaa tcagcagata tcgtgttgat gaaacctgag ttaacagatc
    11581 tcttaaaagc aatgactatc agtaaacaaa cgattcaaat tattaaggaa aatttatttt
    11641 gggctttctt ttataacgtt ttagcgattc cagtggcaat gggtgtttta catctttttg
    11701 gtggtccctt actcaatcct atgttggcag gcttagctat ggcgtttagt tcagtttcag
    11761 tagttttaaa cgcacttcgc ttaaaagtct taaaataata aagataataa gactgaattt
    11821 ttatgaaggg agaagaatat gaaacatacc tatcgagtta gtggcatgaa atgtgatggt
    11881 tgtgccaaaa cagtttctga taagttatca agtgtgattg gtgttgacga ggttaatgtt
    11941 gatttgacta aaaatcaagt tgtcgtaagt ggaaaaacat tcaaatgtct tttaaaacgt
    12001 tctttaaaag acacaaagta ttcactagaa gaggaaataa attaataact aaagaagtgg
    12061 ttacagcttt taatttctta atttgagcag tttctcactt cttttttatt ctaaaaatga
    12121 taaactagaa tagaattagt tgtgaggagt gcagatgtca attgatattt ggaacatttt
    12181 aagtttggtt ggaaccgtag cttttgcaag ttcgggagct attgtggcga ttgaggaaga
    12241 atttgatatt ctagggcttt ttatcttagg ttttgtaacg gcatttggtg gtggagctat
    12301 tagaaatgta ctaattggct tgcctattga aacactttgg agtcaaggaa ttgcttttta
    12361 tgcagcagca gcagctattt tatttattat gattttccct aatttgttga gtggtaaggg
    12421 acgggatgcc gaagttgtat cagatgctat tgggttagca gcgtttagtg ttcaaggagc
    12481 actatatgcg actcaatctc atcaacctct tagtgctgtc attgtcgcag cagtactaac
    12541 tggtgcaggt ggtggtattg tcagagatgt tctggctggc cgaaaacctg gagtactaag
    12601 aagtgaaata tatgcaggtt ggtcaatatt agtggggatc attttatatt ttaaaattgc
    12661 taaaacaaca actgactatt atttgttagt gttagttgta actagcctaa ggatgttggg
    12721 ttataagaaa caatggcact tgccagttgt cagaccacgt aagaaatgat agaggagaaa
    12781 aagatgataa aattaattgc tattgatatg gatggaacgc ttttaaatga tgaaaagaaa
    12841 attccaaagg aaaatatcca agctattaaa gaagcaactc aagcaggtat taaaattgta
    12901 ctgtgtactg ggcgaccgat gtcaggtata cttccttatt ttaatgaact aggtttaaca
    12961 aaagaggagt atattatcat gaataatggt tgtagcactt atagcacgaa agattggcaa
    13021 ttgattgatt ctgcaacttt aacacatgat gaactcatct ttcttgagga ggttgttaaa
    13081 gaatttccca acgtttgttt aacattaaca gctgaaaata cattttacgc tgttggagag
    13141 gaagttcctg aaattgttgc ttatgatgct gatttagttt ttacaaaagc aaagtcaaca
    13201 agtttagatg ctcttcgcaa tcaagaagaa attgtctttc aagcaatgta catgggatta
    13261 gatgcagatg taactgcttt tcaagaagct gttgaagaag ccttaatctc gaaatttagt
    13321 ggagttcgaa gccaagacta tatttatgag attatgcctc aaggagtaac aaaagcaaga
    13381 ggcttaaaat ccttgatagc aaaattagga ctggatatta atcaagttat ggccatcggt
    13441 gatgctccta acgatattga attattagat ttagttccca atagcgttgc tatggggaat
    13501 gcatcagatg agattaaatc tagatgtaaa tatataacgg tggataataa caaagcaggg
    13561 gttgctaaag ctatttatga ctatgcttta cgttaaaata gatatagaat aataaaggga
    13621 actttaaaca ctgtttgttt agggttcttt tgtttaataa actaacaaaa ttcagaagtt
    13681 tacagatagc ttatgctata atagacctat gacaaacaag aataaattac ttttaattga
    13741 tggctcatct gtagcctttc gtgctttttt tgctctttat aatcaaattg accgctttaa
    13801 aaacaattct ggacttcata ctaatgccat atatggtttc catttgatgt tgaatcatat
    13861 tttaggtcgt gtccagccta gtcatatttt agtggctttt gatgccggaa aaactacttt
    13921 ccgtacggaa atgtatgcag attataaagg tgggcgtgct aaaactccgg atgaatttcg
    13981 tgaacaattt ccttacattc gtcaacaatt agatgtctta ggcatcaaac attatgaatt
    14041 ggaacattat gaagccgatg atatcattgg tactcttgca aaacaagccg aagctagtaa
    14101 tgaacatttt gatataacgg tggtttctgg ggataaagat ttaattcaat taacagacac
    14161 taacacggta gtagaaatct ctaaaaaggg agttgctgag tttgaagaat tcacgccagc
    14221 ttaccttatg gaaaaaatgg gaattacgcc gagtcaattt attgatttaa aagctttgat
    14281 gggagataaa tcagataata ttcctggggt tactaaaatc ggtgaaaaaa ctggcttgaa
    14341 gttgctatca gaatatggtt ccttagaagg gatctatgaa aatattgagg ctatgaaaca
    14401 gtctaagatg aaagaaaacc ttatcaatga taaggagcaa gctttcttat caaagaccct
    14461 agctacaatc aacatagcat cacctataac aatcggctta gaagatatcc tttatagtgg
    14521 accgcaagat ataaaagcct tgtctcaatt ttatgatgag atggatttta aacagtttaa
    14581 agcagctctg ggtgaggaga caagtcagga agattttgag gttgacttta ctgaagtgga
    14641 gcaattaaaa acagaaatgt tctccgataa tgatttttat tactttgaga tgttgggtga
    14701 taactaccat gtagaagatt taattgggat agcttgggga aatagcgata caatctacgc
    14761 tactagtaat gtatcgttat tgcaagaagc tttatttaaa aaagctttat caaaacctat
    14821 caaaacttat gattttaaaa gaagtaaggt ccttttaaat cgttttaata ttgatttacc
    14881 agagccagcc tttgatacta gattggccaa gtaccttttg tctactactg aggataatct
    14941 agtatcaaca attgctaggt tatatacaaa tctgccactg gatactgatg atgctgttta
    15001 tgggaaaggt gctaaacgcg ctataccaga aaaaacacga tttcttgagc atctagctaa
    15061 aaaagtaaaa gttttagtgg attctgaggc taatattatg caacagctta aagctaatga
    15121 gcaagaagaa ttgctgtttg aaatggagca acctttagcg aatgttttag ctaaaatgga
    15181 aattcgaggt attaaggtta agaaaaatac cttgaatgaa atggccattg aaaatcaaaa
    15241 agtcatcgaa acattaacac aagaaatcta tgaattggct gggcaagagt ttaatattaa
    15301 ttctccaaaa cagttaggaa agttgttgtt tgaaacgtta ggtcttcctg tagaaatgac
    15361 taaaaaaaca aaaacaggtt attcaactgc agtagatgta ttagagcgct tagctccgat
    15421 ttctcctctt gtaactaaaa ttttagagta ccgtcaaatc actaaattgc aatcaaccta
    15481 tattattggt ttacaagatt atattttaga agatggtaaa attcatacac gttatgttca
    15541 agatttgact cagacaggtc gtctttcaag ttctgaccca aaccttcaaa atataccagt
    15601 tagactagag caaggacggc ttatccgaaa agcttttgtt ccatcagaag acaatgctgt
    15661 tttattgagt tcagattatt ctcaaataga attgcgcgtg ttagcacata tttcaaaaga
    15721 cgaacatttg atcgctgcct ttaaagaggg agctgatatt catacttcaa cagctatgcg
    15781 tgtttttggt atcgaaaaac ctgagaatgt cactcccaat gatcgtcgaa atgctaaggc
    15841 tgttaatttt ggaattgttt atggtatttc agattttggt ctatcgcata atttaggaat
    15901 acctcgtaaa ctagcgaagc aatatattga cacctatttt gagcgttatc ctggaataaa
    15961 aaactatatg gaaacagttg ttcgtgaagc aaaagataag ggttacgttg aaacactttt
    16021 ccatcgtcgt cgttcattac cagatattaa ttctcgtaat tttaacattc gccaatttgc
    16081 agagcgcaca gcaatcaatt caccaatcca aggaagtgca gctgatattc ttaaaattgc
    16141 aatgataaat ttagataggg tacttgataa aggtggttat aaatcaaaaa tgctgttgca
    16201 agttcatgat gaaattgtac ttgaagtccc taacgaggag ataggagcaa tcagggagtt
    16261 ggttacaaaa acaatggagt cagcaattag tttatcggta cctttgattg cagatgagaa
    16321 cgcaggtgag acttggtatg aagcgaagta gtggacaaca tacgtaaagg agtcattaat
    16381 atggtttatc attttcaaaa tccaagtgat tttatgttaa aaaattattt aacaaaagca
    16441 aagaccatcg cagttgtagg actgtcagac cgtcaagaaa ctgcagctta tcaagtatca
    16501 aaaataatgc aagaagcagg ttatcaaatt attccagtta atcctaaaaa tgcaggacag
    16561 aaaatacttg ggcaaatgac atatgcctca cttaaggatg tcactgaaca tattgatatt
    16621 gttaatattt ttcgacgtag cgagtattta cctgatattg caagagaatt tttagaagta
    16681 gacgctgata ttttctgggc tcagcttgga cttgaaagtc aagaagcaga gacaattcta
    16741 aagcaagcag gacacaaaca gattgtcatg aataaatgtc tgaaagttga atgccaaaaa
    16801 ttacactcag aaactaacta attaccgtgt ttaataggca aataatataa attatgctat
    16861 aataaccatg ctaaattaaa gttcgtgctg gaaaggagtc ctatggacag tcattcacat
    16921 ggtcatagac ctctcgatgc ttatgaaaac gtcctagagc atctccgtga aaaacgtatt
    16981 cgaattactg agactcgaaa agctattatt tcctatatgg ttaatagtcg tgagcatccg
    17041 agtgcagaaa agatttacaa tgatttatta ccagaatatc ctaacatgag cttggcaaca
    17101 gtttacaata accttaaggt ccttgtggat gaggggtttg tgacagagct aaaactttgc
    17161 aattatagta caacttatta tgattttatg gggcatcagc acctaaacat tgcctgtgaa
    17221 gattgtggga aaatcgttga ttttgtcgat gttgacttat tggatatttc tagagaagca
    17281 caccaacaaa caggttttga agtgactaga gttcagttag tagcttatgg tatctgtccg
    17341 gagtgtcaga ggaagcgtaa ggataaatat aaaaaagatt gaaacgtaat tgttcaatct
    17401 ttttttgttt atagactaag agaaaaagtt taagttttac taagtaataa ttaagaaata
    17461 atttataatt gcttaaaaaa tgatttattt tttgttggta tactctcatt aacaaaaaga
    17521 aagaggaatc ccttatgaaa atatcacaat acaataaatg gtcaattcgc cgattgaaag
    17581 ttggagctgc atctgtaatg attgctagtg gaagcattgt tgctttaggg caatctcata
    17641 ttgtttcagc agatgagatg tcccaaccta aaacaaccat tacagctcca acagctaaca
    17701 cttctacaaa tgtagaaagt agtactgata aggcattatc aaaagttact acgatggaga
    17761 caagttcaga aatgcctaaa atgcaaaata tggcaaaagt tgaaaaaacg agtgacaaac
    17821 ctatgatggt ggctacctct gttagaaaaa tgatggcaac gccgacacct gtagcgatga
    17881 ccaaaacgac tagtgtagat gaggtaaaga aatcaactga tactgctttt aaacagacag
    17941 tagacgttcc ggctcattat gttaatgcag caaaagggaa tggcccattt ctagcaggtg
    18001 taaatcaaac gattccatat gaagcgtttg gtggagatgg catgttaacg cgtttaatcc
    18061 ttaaatcttc tgaaggagca aaatggtcag ataatggtgt tgataaaaat agtccattat
    18121 taccattgaa aggcttaact aaaggtaaat atttctatca agtttcttta aatggtaata
    18181 cgacaggtaa agagggacaa gctctcttag atcaaattaa agctaatgat aaacatagct
    18241 accaagccac aattagagtt tatggtgcta aagatggtaa agttgattta aaaaacatga
    18301 ttagccaaaa aatggtaacc attaacattc ctcacattac aactgatatg gaagtaaaaa
    18361 attctctaaa gatggccttt aaagaaaaag tagacgtccc agcaaaatat gttagtgctg
    18421 caaaagctaa aggtccattt ttagcaggtg ttaacgaaac gattccatat gaagcgtttg
    18481 gtggggatgg catgttaacg cgtttaatcc ttaaagcttc agagggagca aaatggtcag
    18541 acaatggtgt tgataaaaat agtccattat taccgttaaa agatttaact aagggtaaat
    18601 acttctacca agtctctctt aatggcaata cagccggtaa aaaaggccaa gctctcttag
    18661 atcaaattaa ggcaaatgga agccatactt atcaagcaac tatcactatt tatggtacga
    18721 aagatggcaa agttgatatg aatactattt taggacaaaa gacagtgatg attcatatta
    18781 atgtcgctaa aaaagatatg aatagcacat caatgatgat gaaaaaagat aagatgacaa
    18841 tgcctatgaa gaaagaaatg acttcatcta aaataaatac cggtatgatg atgtctaaca
    18901 acaagatgtc agctaatatg caaatgtcat cacaagccaa atcaaatgat aaagctggta
    18961 aaaaaatgtc tatgatgtct aaaaatttac ctaatacagg tgaaacaaag caacaaaatg
    19021 taggtgtgct aggtatgctt agtctagcct ttgcgacagg tctaactgcg ctaggcctta
    19081 aaaaatctaa acaaagatag ggatagtatt taattcagaa caccaaattt gtttaaaatt
    19141 attgagggtt atcgtggtaa aatagtgtta tactagaggt atagcactat tttgactcat
    19201 taggagagta gaatgacaca aaaattatta ttagttgacg atgaatttga aattattgat
    19261 attaatagac gttatctaga gcaagcagga tatgaagtgt cagtagctgc ggatggcata
    19321 gaagcactca aagaggtaga tgagaatcgt tttgacctaa ttattagtga tattatgatg
    19381 cctaaaatgg atggctatga ttttattagt gaagttctag ttcgagaacc caaccaacct
    19441 tttctcttta taaccgctaa agtatctgaa ccagataaaa tttactcttt gagtatgggt
    19501 gcagatgact ttatttccaa gcctttcagt cctagggaat tagttttacg tgttaaaaat
    19561 attctaagac gtatctatgg gaatcatcaa caatctgaag tgttgacgat tggtgatttg
    19621 gttatcgacc aaaaacaacg tttggtaatg gtggactgca atacaatttc cttaaccaat
    19681 aaatcatttg acttgctttg gatattagca aatcatttga atcgtgtttt ttcaaaaaca
    19741 gagctttatg aacgtgtctg gggcgaagaa tttttagatg acactaatac tttgaatgtt
    19801 catatccatg ccttacgcaa tgaccttgct aaatttagta cagacaatac cccaactatc
    19861 aaaacagttt ggggattagg gtataaatta gaggaataaa atgaaattaa aatattatat
    19921 tgttattggt taccttattt ctatgttaat aactgtagca ggtgttttct ttgggcttaa
    19981 tcatatgctg attgagactc gaggtgttta ctacatcctt tcagttacaa ttattgcctg
    20041 tattgtaggt ggtatagtta atttgttctt attatcaagt gtatttacat ctttgaaaaa
    20101 gttaaaacaa aaaatgaagg atatttctca aagatgtttt gatacaaaag cacaaatttg
    20161 ttcaccacaa gaattcaagg atttagagac agcctttaac caaatgtctt cggagttaga
    20221 gtcaacgttt aaatctctaa acgagagtga acgcgaaaaa acaatgatga ttgcacaact
    20281 ttcacatgat atcaagactc caattacttc tatacaatca acagtagaag gtattttaga
    20341 tggtattata tctgaagaag aagttaacta ttatctcaat accatttctc gtcaaacaaa
    20401 ccgtcttaac catctggttg aagaattaag ttttattaca ttagagacta tgtcagacac
    20461 ggctgaatct cataaagaag aaacaattta tctggataaa ctactgattg atattttatc
    20521 tgaatttcaa ttggtgtttg aaaaagaaaa tcgccaagta atgattgatg ttgcgccaga
    20581 tgtttcgaaa ttatctagtc aatatgataa attatcacga attttattga atttgattag
    20641 caatgcagtc aaatattctg atcctggaag tcccttgact atcaaggcct attcaaatcg
    20701 tcaagatatc gttatcgata ttattgacca aggctatggg ataaaagatg aagatcttgc
    20761 ttctattttt aatcgtcttt atcgtgtaga atcttcacga aatatgaaga caggaggaca
    20821 tggtctaggt ctttatattg cgcgacaact tgcacatcaa ctgaatgggg atattttggt
    20881 cgaaagtcag tatcaaaaag gcagtaagtt ctctttagta cttaaattac aaaaatagct
    20941 aggaattatt cctagctatt ttttgtaatg tttctataaa agattaagcg cgcagtagtt
    21001 tggtaaggaa ggagatagat atagactaat ccgaaactga agattgtaag gaactgccaa
    21061 ccaataaaac ttaagtctaa aaggaagagt ctaagtttat taccattcat tagttctcga
    21121 ctagcaacca gtactttaga cggtccctca tagtttccac tttctaattg agtgtaaaat
    21181 actaaagttg agatactata ggagtaatat tgttgaacga gttttataac tgccttagta
    21241 gtaattaggc tactaacaac tattaaaaca ggggcaagga taaggttacc actcaaaagt
    21301 agcctaaaac ttgaaatgat gattagtaga ccaaaagtac ttagtagata aaagaaagat
    21361 aaaattagtc cttttagaac aagtactgag agtaatttcc agaaaatatg gcttgaaaat
    21421 acataagtat tgtccgagaa tctaacgttt agtttctgat ttttgacgac atctaacatt
    21481 acaaaactga ctgaaataat aaaaagtgtg taaaagaaat aaactggaag aggtacaaaa
    21541 aaatcaagta gtgtcatatt attatattga taataatatg atatatataa cgtaataata
    21601 ctaaggagaa tcgggagtaa aaaaagttga ttcttacctt gaagattgct taatatagtt
    21661 tttgcctcat ttttaatttc tgaattcgtc atatctcctc catacacaaa agtacaattc
    21721 atttcattat aacacattcc ttaagatgag catgacaaga gtacattttt ttgataaaat
    21781 cttaatggct aaaaaagaaa ttaatacaaa gtattacatt tagatattga ttagcactaa
    21841 agcccatgac tgtttttcat ggaagcaagg agaaaacgat gactgatcat ccaataaaat
    21901 atcgattaat taaacaggaa aaacacacag gagccagact tggtgaaata atcacaccac
    21961 atggtacatt tccgacacca atgtttatgc ctgttgggac acaagcaact gttaagacac
    22021 aatctcctga agaactaaaa gaaatgggtt caggaattat tctatcaaat acctaccatt
    22081 tatggttacg tccaggtgat gaattgattg ctaaagctgg aggtttacat aaattcatga
    22141 actgggatca ggctattcta acagacagtg gaggctttca ggtttattct ttggctgata
    22201 gtcgaaacat cactgaagaa ggagtaacct ttaaaaacca tttaaatggt gcgaaaatgt
    22261 ttttatcacc agaaaaagct atttcaattc aaaataacct tggttctgat attatgatga
    22321 gttttgatga gtgtccacaa ttttaccagc cttatgatta tgtaaaaaaa tcaattgagc
    22381 gaacaagccg ttgggcagaa cgtggattaa atgcccaccg ccgtcctcat gatcaaggac
    22441 tatttggtat tgttcaaggg gcagggtttg aggatttacg tcgtcaatca gcacgagatt
    22501 tagtatcgat ggattttcca ggttattcaa ttggagggct tgcggtcgga gaaacacatg
    22561 atgagatgaa tgcagttctt gattttactg ttccaatgct tccgaatgat aagcctcgtt
    22621 atttgatggg agttggtgca ccagatagtt taatagacgc cgttatccga ggagtagata
    22681 tgtttgattg cgtcttacct actcgtattg ctcgaaatgg gacatgtatg acaagtcaag
    22741 gtcgcttagt tgttaagaat gctaaatttg cagaagattt tacgcctctt gatccaaatt
    22801 gcgattgcta tacttgtaaa aattatacac gcgcttatat tcgtcactta ttgaaagcag
    22861 atgaaacttt tggtattcgt ttgactagtt accataatct ttatttcctt gtcaatctta
    22921 tgaaagatgt cagacaagct attatggatg ataatcttct agaattccgt caagatttta
    22981 tggagcgcta tggctatggc atgaataacc gtaattttta aaaaagttcc tggtaaaaca
    23041 gggctttttt ttgaattgtt tgttaaccgt tttttaaaaa aagttgacaa tagaaaaact
    23101 tttgtataat atgttatagg aggaatcatg atgcaagagt actttggtat tggtcttgat
    23161 agtagcagcc gctgttacca ctatcatact aaattggata ttgtcgcttt aaaatgtgct
    23221 gtttgtcaaa aatattatgc ttgttataaa tgtcatgatg cactcgagga gcattgtttt
    23281 gcagcaacaa aatctgatga aacgtttcct gtcttatgcg gttcttgtcg tcagatgttg
    23341 acattaaaag agtataaaac tggtttttgt ccttattgtc gtatgctttt taatccaaat
    23401 tgtcaacgcc atgattctat ttatttttcg aaggagtaat ttttaatgtt tcacacaaaa
    23461 agcctaatcc agatggctat gatggctact ttattggtgg tacttggttt cattcctgct
    23521 ctaccacttg gtttcattcc ggtccctatc gtcttacaaa acctaggggt tatgatggct
    23581 ggtgttattt tgggcggaag aaaagggagt ttatctattt tgctcttctt tcttgtcggt
    23641 ttagttattc ctgtattctc aggtttacgt tcgactatac ctgtattgac aggaccaaca
    23701 gctggttatg ttattgcttg gttgtttgtt cctttgctta tctcatatgg tgtaaaatta
    23761 gtaaataaaa agaattttat agctattttc cttattgtat ggctagcagg ggtccttttt
    23821 gtagatgtag caggagcagt ttggctggca tcttatacga acatcccatt agataaatca
    23881 cttcttcaaa acttagtatt tatcccagga gatacaataa aagccattat agctgctgtt
    23941 gttgctgtta aatataaaga tagttttttc ttgactgaaa aataatcaag attgctatat
    24001 attagatgga ctagtaaatc taaaaattta gatttactag tcttttttgt gcataaaatc
    24061 taaaacagtt attcttaata aaatttggta taatagcatt aaatcagtaa ggaggggatt
    24121 atcatgaaat taacaacgtt aggttgttgg ggtggttatc cttataaaga tggaggaacc
    24181 acaagttttc tattaactgg agaaaataat tttcacttat taatagatgc gggaagtcgt
    24241 tcaatcaatg agctagaaaa agaaataagc ccgttggatt tggatgcagt tatcatcagt
    24301 cattatcatc cagatcatgt agcagatttg ggtgtgttgt atcattatcg tttgctatac
    24361 cctaaagata aatgggaacc caaagtattg ccagtttatg gacataatga ggatgattat
    24421 gagttttcta aattgacatt acctaacgtt gctcaaggaa ttgcttatca taccaatgga
    24481 gttgagcaag ttggtccctt caatattacg tttatcaaaa cagtccaccc agtcgtctgc
    24541 tatgccatga ggattacaga aagagaaact ggtcaagtat tagtctttac aggagattct
    24601 gggtattttg ctgaccttgt accttttaca aaaggcgcag atttattttt agcagacgtt
    24661 tatctttacg agggaaatga aaatcatatt gcacatttga ctagtaaaga agcaggtgag
    24721 attgcttata acgctaaagt taagaaatta gttttaacac atgttccgca gtttccggga
    24781 gaaggaataa accccgaaaa tcatttggaa gaacttcgac tacagtctga aaagtatgca
    24841 ggtggcatct ctgttgattt agctcagcca cataaatctt gggacttagg aaaactataa
    24901 tgcagaaatt tacccaagaa gaaaaagaat ggtttatgac agaagctcta aaggaggcaa
    24961 aatcctcctt agagaaagaa gaaattccta taggttgtgt cattgtaaaa gatggtcata
    25021 ttattggaag aggacataat gctcgtgaag agtttaataa agctatttta catgctgaaa
    25081 tcatggctat taacaatgca aatgagaagg tagggaattg gcgcttatta gatactactt
    25141 tatttgttac tgtagaacct tgtgtaatgt gtagtggagc tattggctta gctagaattc
    25201 cccacgttat atatggcgct aagaatacga aatttggagc agcgggaagt ctctatgata
    25261 ttttaagtga tagccgactt aatcatagag ttgaggttga aaccggcatt ttagaagatc
    25321 aatgcgctaa aataatgcag gatttctttc gaaaaggacg acaacgcaag aaagaagcta
    25381 aactaagtga aaaaactaag atttgatagc ttgcaataaa atattagttt tgttataata
    25441 tctttggagc aacatttact cgtgaagtgg gtcaggggag gaat
//
LOCUS       DATRSD010000023        25014 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRSD010000023 DATRSD010000000
VERSION     DATRSD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25014
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.023"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            36..3440
                     /locus_tag="VBD85_000751"
     CDS             36..3440
                     /locus_tag="VBD85_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO7133316.1"
                     /translation="MFKSNYERKMRYSIRKFSVGVASVAVASLFMGSVAHASELVKDD
                     SVKTTEVAAKPYPSMAQTDQGNNSSSSELETTKMEIPTTDIKKAVEPVEKTAGETSAT
                     DTGKREKQLQQWKNNLKNDVDNTILSHEQKNEFKTKIDETNDSDALLELENQFNETNR
                     LLHIKQHEEVEKDKKAKQQKTLKQSDTKVDLSNIDKELNHQKSQVEKMAEQKGITNED
                     KDSMLKKIEDIRKQAQQADKKEDAEVKVREELGKLFSSTKAGLDQEIQEHVKKETSSE
                     ENTQKVDEHYANSLQNLAQKSLEELDKATTNEQATQVKNQFLENAQKLKEIQPLIKET
                     NVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREYEGKLNQSKNLPELKQLE
                     EEAHSKLKQVVEDFRKKFKTSEQVTPKKRVKRDLAANENNQQKIELTVSPENITVYEG
                     EDVKFTVTAKSDSKTTLDFSDLLTKYNPSVSDRISTNYKTNTDNHKIAEITIKNLKLN
                     ESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQVPKTPEQKDSKTEEKVPQEPKSNDKN
                     QLQELIKSAQQELEKLEKAIKELMEQPEIPSNPEYGIQKSIWESQKEPIQEAITSFKK
                     IIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTRKVVQYMNKYPDNAEIKKIFESD
                     MKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDDLDKKVEQDQPAPIPENSEMD
                     QAKEKAKIAVSKYMSKVLDGVHQHLQKKNHSKIVDLFKELEAIKQQTIFDIDNAKTEV
                     EIDNLVHDAFSKMNATVAKFQKGLETNTPETPDTPKIPELPQAPDTPQAPDTPHVPES
                     PKAPEAPRVPESPKTPEAPHVPESPKTPEAPKIPEPPKTPDVPKLPDVPKLPDAPKLP
                     DGLNKVGQAVFTSTDGNTKVTVVFDKPTDADKLHLKEVTTKELADKIAHKTGGGTVRV
                     FDLSLSKGGKETHVNGERTVRLALGQTGSDVHVYHVKENGDLERIPSKVENGQVVFKT
                     NHFSLFAIKTLSKDQNVTPPKQTKPSTQGSQVEIAESQTGKFQSKAANHKALATGNET
                     VAKGNPTSTTEKKLPYTGVASNLVLEIMGLLGLIGTSFIAMKRRKS"
     gene            complement(3570..3734)
                     /locus_tag="VBD85_000752"
     CDS             complement(3570..3734)
                     /locus_tag="VBD85_000752"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7133317.1"
                     /translation="MEATNHLIKLFKYNIFSFRNFENFKKRIFIALNIKKKGQNASFL
                     VCSLVSTNYS"
     gene            complement(4091..5113)
                     /locus_tag="VBD85_000753"
     CDS             complement(4091..5113)
                     /locus_tag="VBD85_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag3 family transposase"
                     /protein_id="HEO7133318.1"
                     /translation="MSTNHSTKKSLYSHLSASERGEISAYLKMGKKPSEIAHLLGRHR
                     STISREIKRGSVSQVQDKNGKRIYSTVYFPDSGQRVYETNRRKSAYHKLSYCSQTFFK
                     ELEKALKTKPRCHSVDSFVQTYQEKPPLEVIPSTKTVYRYIKDGLLRVKPIDLPKMVC
                     IRKRAKVTPKATKKILGKSIEERPETITNRSEFGHWEIDLMLGKKTKGEAVVMTLVER
                     QTRFVIAVKLANKQAETINRAVKSLLSQYPIRSIASDNGSEFSSLSDLKGVEVYFAHP
                     YASHERGTNENFNGLLREFLPKGVSLNSLTTEELNLYVSAINDRPRRIQKYKTANILF
                     GLAQTA"
     gene            5306..5422
                     /locus_tag="VBD85_000754"
     CDS             5306..5422
                     /locus_tag="VBD85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO7133319.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(5514..6398)
                     /locus_tag="VBD85_000755"
     CDS             complement(5514..6398)
                     /locus_tag="VBD85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO7133320.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(6546..8210)
                     /locus_tag="VBD85_000756"
     CDS             complement(6546..8210)
                     /locus_tag="VBD85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO7133321.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDVLGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(8210..8575)
                     /locus_tag="VBD85_000757"
     CDS             complement(8210..8575)
                     /locus_tag="VBD85_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7133322.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSTTEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(8707..8895)
                     /locus_tag="VBD85_000758"
     CDS             complement(8707..8895)
                     /locus_tag="VBD85_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO7133323.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            9134..10051
                     /locus_tag="VBD85_000759"
     CDS             9134..10051
                     /locus_tag="VBD85_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7133324.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(10136..11017)
                     /locus_tag="VBD85_000760"
     CDS             complement(10136..11017)
                     /locus_tag="VBD85_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO7133325.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(11158..12546)
                     /gene="argH"
                     /locus_tag="VBD85_000761"
     CDS             complement(11158..12546)
                     /gene="argH"
                     /locus_tag="VBD85_000761"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO7133326.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRITLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLETMTVNEHIMMTSTETDFSNATELADYLASKGIPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKK
                     QILLARKELKAE"
     gene            complement(12565..13755)
                     /locus_tag="VBD85_000762"
     CDS             complement(12565..13755)
                     /locus_tag="VBD85_000762"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO7133327.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWKWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLSDLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAQVVARHSSNSLYDENLATYTAADSFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            complement(13909..14988)
                     /locus_tag="VBD85_000763"
     CDS             complement(13909..14988)
                     /locus_tag="VBD85_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7133328.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVADGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDYMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSDDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGRISVSSQLEIGTEFCVELPLS"
     gene            complement(14972..15643)
                     /locus_tag="VBD85_000764"
     CDS             complement(14972..15643)
                     /locus_tag="VBD85_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133329.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(15674..16360)
                     /locus_tag="VBD85_000765"
     CDS             complement(15674..16360)
                     /locus_tag="VBD85_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133330.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(16360..17448)
                     /locus_tag="VBD85_000766"
     CDS             complement(16360..17448)
                     /locus_tag="VBD85_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133331.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDGLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            17630..18616
                     /locus_tag="VBD85_000767"
     CDS             17630..18616
                     /locus_tag="VBD85_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7133332.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGACFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            18609..19520
                     /gene="rbsK"
                     /locus_tag="VBD85_000768"
     CDS             18609..19520
                     /gene="rbsK"
                     /locus_tag="VBD85_000768"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO7133333.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWAIIKKADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRETDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWHIK"
     gene            19495..19893
                     /gene="rbsD"
                     /locus_tag="VBD85_000769"
     CDS             19495..19893
                     /gene="rbsD"
                     /locus_tag="VBD85_000769"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO7133334.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGVPSFQEVLDIYLENILVEKVILAEEIKEANPDQLSRLLAKLDNSVSIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            19909..21387
                     /locus_tag="VBD85_000770"
     CDS             19909..21387
                     /locus_tag="VBD85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO7133335.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLSQLSI
                     LVMDEPTAALTDRETENLFRVIRSLKQEGVGIVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDDQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            21389..22330
                     /gene="rbsC"
                     /locus_tag="VBD85_000771"
     CDS             21389..22330
                     /gene="rbsC"
                     /locus_tag="VBD85_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO7133336.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            22383..23351
                     /locus_tag="VBD85_000772"
     CDS             22383..23351
                     /locus_tag="VBD85_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7133337.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSENSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDMLSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(23391..24845)
                     /locus_tag="VBD85_000773"
     CDS             complement(23391..24845)
                     /locus_tag="VBD85_000773"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO7133338.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     SESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSVFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         8071 a         4693 c         4381 g         7869 t
ORIGIN      
        1 tactgtagat aaataaaata ttggaggata tcgatatgtt taaatctaat tatgaaagaa
       61 aaatgcgtta ttccattcgt aaatttagtg taggagtagc tagtgtagcg gtagctagtt
      121 tgttcatggg aagcgttgct catgcaagtg agcttgtaaa ggacgatagt gtgaagacta
      181 ccgaggttgc agctaagccc tatccaagta tggctcaaac agatcaagga aataattcat
      241 catcctcgga acttgagaca acaaagatgg aaattcctac aacagacata aaaaaagctg
      301 ttgaaccggt cgagaaaaca gctggggaaa catctgccac tgatactgga aaacgagaga
      361 aacaattaca acaatggaaa aataatctaa aaaatgatgt ggataacaca attctatctc
      421 atgaacagaa aaatgagttt aaaacaaaaa ttgatgaaac aaatgattct gatgcattat
      481 tagaattaga aaatcaattt aacgaaacta atagactgtt acacatcaaa caacatgaag
      541 aagttgagaa agataagaaa gctaagcaac agaaaactct gaaacagtca gatacgaaag
      601 tagatctaag caatattgac aaagagctta atcatcaaaa aagtcaagtt gaaaaaatgg
      661 cagagcaaaa gggaatcaca aatgaagata aagattctat gctgaaaaaa atcgaagata
      721 ttcgtaaaca agctcaacaa gcagataaaa aagaagatgc cgaagtaaag gttcgtgaag
      781 aactaggtaa actctttagt tcaactaaag ctggtctgga tcaagaaatt caagagcatg
      841 tgaagaaaga aacgagtagt gaggaaaata ctcagaaagt tgatgaacac tatgctaata
      901 gccttcagaa ccttgctcaa aaatctcttg aagaactaga taaggcaact accaatgaac
      961 aagctacaca agttaaaaat caattcttag aaaacgctca aaagctcaaa gaaatacaac
     1021 ctcttatcaa agaaacgaat gtgaaattgt ataaggctat gagtgagagc ttggagcagg
     1081 ttgagaagga attaaaacat aattcggaag ctaatttaga agatttggtt gcgaaatcta
     1141 aagaaatcgt aagagaatac gaaggaaaac ttaatcaatc taaaaatctt ccagaattaa
     1201 agcaactaga agaggaagct cattcgaagt tgaaacaagt tgtggaggat tttagaaaaa
     1261 aatttaaaac gtcagagcaa gtgacaccaa aaaaacgtgt caaacgagat ttagctgcta
     1321 atgaaaataa tcaacaaaag attgagttaa cagtttcacc agagaatatc actgtatatg
     1381 aaggtgaaga cgtgaaattt acagtcacag ctaaaagtga ttcgaagacg acgttggact
     1441 tcagtgatct tttaacaaaa tataatccgt ctgtatcaga tagaattagt acaaattata
     1501 agactaacac ggataatcat aagattgccg aaatcactat caagaatttg aagctaaatg
     1561 aaagtcaaac agtgactcta aaagctaaag atgattctgg caatgtagtt gaaaaaacat
     1621 tcactattac agtgcaaaag aaagaggaga aacaagttcc taaaacacca gagcagaaag
     1681 attctaaaac ggaagaaaag gttcctcaag aaccaaaatc aaatgacaag aatcaattac
     1741 aagagttgat taaatcagct caacaagaac tggaaaagtt agaaaaagca ataaaagaat
     1801 taatggagca accagagatt ccatccaatc cagagtatgg tattcaaaaa tctatttggg
     1861 agtcacaaaa agagcctatc caggaagcca taacaagttt taagaagatt attggtgatt
     1921 catcttcaaa atactacaca gagcactatt ttaacaaata taaatctgat tttatgaatt
     1981 atcaacttca tgcacaaatg gagatgctga ctagaaaagt ggttcagtat atgaacaaat
     2041 atcctgataa tgcagaaatt aaaaagatat ttgagtcaga tatgaagaga acgaaagaag
     2101 ataattacgg aagtttagaa aatgatgctt tgaaaggcta ttttgagaaa tatttcctta
     2161 caccatttaa taaaattaag cagattgtag atgatttgga taaaaaagta gaacaagatc
     2221 agccagcacc aattccggaa aattcagaaa tggatcaggc taaggaaaag gctaagattg
     2281 ctgtatcgaa gtatatgagt aaggttttag atggagttca tcaacatctg cagaagaaaa
     2341 atcacagtaa aattgttgat ctttttaagg aacttgaagc gattaaacaa caaactattt
     2401 ttgatattga caatgcaaag actgaagtag agattgataa cttagtacac gatgcattct
     2461 caaaaatgaa tgctactgtt gctaaatttc aaaaaggtct agagacaaat acgccagaaa
     2521 ctccagatac accgaagatt ccagagctac ctcaagcccc agatacaccg caggctccag
     2581 acacaccgca tgttccggaa tcaccaaagg ccccagaagc accgcgtgtt ccggaatcac
     2641 caaagactcc agaagcaccg catgttccgg aatcaccaaa gactccagaa gcaccaaaga
     2701 ttccggaacc ccctaagact ccagacgtcc ctaagcttcc agacgtccct aagcttccag
     2761 atgcaccgaa gttaccagat gggttaaata aagttggaca agcagtattt acatcaactg
     2821 atggaaatac taaggttacg gttgtatttg ataaacctac agatgctgat aagttacatc
     2881 tcaaggaagt aacgacgaaa gagttggctg ataaaattgc tcataaaaca ggaggaggaa
     2941 cagttcgtgt gtttgactta tctctttcta aaggaggcaa ggaaacacat gtcaatggag
     3001 aacgaactgt tcggctcgcg cttgggcaga ctggctcaga tgttcacgtc tatcacgtaa
     3061 aggaaaatgg cgaccttgag cgtattcctt ctaaagttga aaatgggcaa gttgttttta
     3121 aaacgaacca cttcagtttg tttgcgatta agacactttc taaggatcaa aatgttactc
     3181 caccgaagca gactaaacct tctacccaag gcagtcaagt agagattgca gagagtcaaa
     3241 ctggaaaatt ccagagtaaa gcagctaatc ataaagcact ggctactgga aatgaaacag
     3301 tggcaaaagg aaatcctaca tcaacaacgg aaaagaaatt gccatataca ggagtggcat
     3361 ctaatctagt tcttgaaatt atgggtctcc ttggtttgat tggaacttca ttcatcgcaa
     3421 tgaaaagaag aaaatcatga ttcagttttt taaaaatatc cactttcgat atctagcatt
     3481 tgattggtta tctgtggatg attctaaaga tgttacctat cgttggtatg taacaattat
     3541 aagtcatttc atataaaaga ggctctttgt caactgtagt tggttgaaac aaggctacaa
     3601 actagaaagg acgcattttg tcctttcttt ttgatgttga gggcaatgaa aatacgcttt
     3661 ttgaagtttt caaaattccg aaaactaaag atattgtatt tgaaaagttt aatgagatga
     3721 ttagttgctt ccaattttgc gttggagtag gtttactgaa ggacgttgac gatattctct
     3781 ttgcttttga gaatgatttt aaagatagtc tgaaaaagag gatgaacttg ctttagaacg
     3841 tcctgaatga gttcgaagaa tttatcaaac ttcttgttct ggaagtaaaa aaacaagatc
     3901 taatggggaa tctcagtcat agaagtcttt tcaatcaatt tttgagcaat tctttggttg
     3961 ataaatacaa aaaacgtgac gattcttctt gataagagga gttttaattg catatatttt
     4021 ttgagtaaat aatatccccc acttgtcaag tcaagtgcaa caagttcatt gataactaga
     4081 gttccagagg ttaagctgtt tgggctagcc caaacaaaat atttgcggtt ttatactttt
     4141 gaattcgtct aggtctgtca ttgatagcag agacgtagag attgagttct tctgtcgtta
     4201 gtgagttaag agagacacct tttgggagaa actctctcaa gagaccattg aaattttcat
     4261 ttgttcctct ttcatgagaa gcataaggat gggcaaaata gacttccaca ccttttaagt
     4321 ctgacaagct actgaactct gagccgttgt ccgatgcgat agagcgaata ggatactgtg
     4381 atagtaagct cttaacagcc ctattgatgg tttctgcttg tttattagcc agttttacag
     4441 cgatgacaaa tcgtgtttga cgctctacta gggtcatgac aacagcttcc cctttggtct
     4501 tcttgccaag catgagatca atttcccaat gtccaaattc agaacgattg gtaatagttt
     4561 ctggacgttc ttcaatggat tttcctaaga ttttcttcgt ggccttaggc gttactttag
     4621 cccgttttcg gatgcacacc atcttaggta aatcaatcgg tttaaccctc aacagtccgt
     4681 ctttgatgta acgatacact gtcttggtgg aagggataac ttccagtgga ggtttttctt
     4741 ggtaagtttg aacaaagcta tcgacactgt gacaacgagg tttcgttttc agggctttct
     4801 caagttcctt gaagaaggtc tgggagcagt acgatagttt atgataggca ctttttcgac
     4861 gattggtttc ataaacacgt tgaccactat ctggaaaata aaccgttgag tagattcgtt
     4921 tcccgttctt atcttgaacc tgagaaacac ttcctcgttt gatttcacga ctgatggttg
     4981 agcgatgacg cccaagcaga tgagcaatct cagagggttt tttgcccatc ttgagatagg
     5041 cgctgatttc tccgcgttca gaggctgaaa ggtgtgagta taacgatttt ttggtagaat
     5101 gattagtgga catgttcatc tgctttctat actgagttgg ggaattctag tatatcagac
     5161 gagcatgtct tttttgttgc acttcatttt acaacgcggg tttgctattt atttttaaat
     5221 ttatcaacaa agtactttac cagttaattt ttttgtggta tactggtgtc tgttaacgtt
     5281 aataaattgt ctataggagg taatcatggc tgaaaaaaca gacctatcgt cagcctatcg
     5341 cagacttaaa agtcctaaca ttaaaactag aaaacgtgct ctgaaaatca ttcatgagta
     5401 caaacgttac ggtaaaaaat aacttttttc cgtctctcca attataataa ataagtcaga
     5461 cacaaaataa ccagagcaaa tgctctggtt attttgcttc tataaaaagt tcattatctt
     5521 gttttaagag cagataaaac ttgtgtacga atatcttcaa caccttctgg gttatttggt
     5581 aagaaaatcg tttgattacc attaatagca aatgtattta aagtatctaa atattgattt
     5641 gttaacaaga tagacatgat ttgttcttct gtcaatgtca cgttagcatc tttcaattct
     5701 tgaatcgaat cagctaaccc atctacaatt gctttacgtt gttgcgcaat acccacacca
     5761 tgtagacgat ctttttctgc ttctgcttct gcagcagtta caattttgat cttatcagca
     5821 ttcgctaatt cttgagctgc tacacgttta cgttgtgcag cattgatttc gttcatggat
     5881 tgttttactt ctgcatctgg ctcaacctta gtaattaaag tcttaacaat gatgtagccg
     5941 tatgttgaca tttcttctgc cacttgatgt tgaacttcca aggctatttc atctttcttt
     6001 tcgaatagct catctaatgt aagtttagga actgatgaac gcaaagcatc ttcgatataa
     6061 gatttaatct gagcttctgg ctttattaat ttgtaataag cgtctgtaac gttattttca
     6121 ttgacacgat attgtgtagc aatatttaaa gtgacaaaaa cattatcttt tgtttttgtc
     6181 tcaactataa tttcactctg taaaagacgt aactgcactc gtgctgcaat cttatcgata
     6241 cccaatggta cacgaatatg aataccgcta gtagcagtct tttgatattt accaaagcgt
     6301 tcaatgatgg ctaccgtctg ttgtttaaca acgtataaag aagttatcaa cagaacaatc
     6361 actaaaacca aaataaccgt aagaataatt aatgacatca taaatctcct ttgtatgaat
     6421 gttttaaaac aattctagca caatttgtca aaattttcaa aatactgatg ctttatcata
     6481 ctaaaataga aactctgata aaataaaaaa ttagaagtat ctctcctaat tttttatttt
     6541 atcaattatt caacgctcat gaggtatgga tatacaggtt gatctccttg gtggatctca
     6601 acttcaacat cttcataagt ctcctcaaga tattccgaca aggtctcggc taatgcttgt
     6661 ttaccatctt caccaacata gattgtcacg atttcactat cttcatcaat cattttatca
     6721 aaagtgtctt taagtgcttt ttccatatct ggtgtagata ctagaatttt accatcaacc
     6781 atcccaagga cgtcgttttc atgaatttca aggccatcta tcgttgtatc acgaacagct
     6841 aaagtcacac taccactaat gacatctgag aggctatttg tcatatcagc tacattagtt
     6901 tcaagagact tagcaggatc aaaagcaagt aagctagtaa atccttgtgg aactgtacgt
     6961 gtctctacta ctgctgctgg aatatcaacg acatctgcag ctgactgtgc agccatgaag
     7021 atgttcttat tgtttggtaa aataataaca tttctagcgt ttactttttc aatcgctttt
     7081 acaatatctt ctgtagatgg gttcatagtc tggccgccag agatgatata atcaacacct
     7141 tgtgatttaa aaatctcagc tagaccgtca ccagctacaa cagcaataat accatattct
     7201 ttagtttctt tgacagtttc ttcaacttca accttttgca tttgggcatc atgttggtta
     7261 cgcatgtttt caactttaac cttcacaagc gatccatatt tcaaaccttc ttgcataaca
     7321 agacctggat cttctgtatg aacatgaacc ttgacaattt catcatcatt aacaactagt
     7381 aaagaatccc ctagattgct aagataacct tgaaattctt cgtagttaaa ttccttaacg
     7441 taagttggtc cttgctttaa accaaccatg acttctgtac aatagccata tttaatatct
     7501 tctgtggcta cgtggcctac tacagcttta tggtgctctg cattaaccat ttcagtcatt
     7561 gttgcgggag ttgctttaaa atcttctgat gcgatatatt ccccagtcag tgctgaaagg
     7621 aaaccttcgt aaataaagac gagtccttga ccaccagaat caacaacacc aacctcttta
     7681 agaacaggta gcatatccgg tgttttagca agagcacgct tagcaccttt gagagttgcg
     7741 cgcataactt caactgcatc atcagtttct tcagcttttt ttagagcagc ggtagctgca
     7801 ccgcgtgata ctgtcaagat agttccttca actggtttca taacggcttt ataagctact
     7861 tcaacaccat tctggaaagc gtgtgccaaa tcttgacccg ttaattcttc tttatccttg
     7921 atactttgac caaaaccacg gaataattgt gatgtaatta cacctgaatt tccacgtgct
     7981 cccatcaata cacctttaga aagaatttga ccaacttctc caacagtcgt cgctggtttg
     8041 tccgaaactt ccttggctcc gttttccata gtcatcccca tattcgtacc agtatcacca
     8101 tctggaactg ggaatacatt taatgagttg acatactctg cctgtttact cagacgtgta
     8161 ctggcagctt gtaccatttc ttggaataaa cttgtcgtaa tctttgacac taatcttctc
     8221 ctacaacctt aatactttgc acataaacat tcaccatatc agcagataaa ccaagctgat
     8281 tttcgagatt gaatttcact cgctcttgaa tattttttga aacttcacta attttgacgc
     8341 cgtaactcat gacagtataa acatctactg cgatgcccgc ttcactagac ctcacaacaa
     8401 caccttttgc gtagttttcc ttacgcaaaa gtgcttgaaa gttatctttg atagcacttt
     8461 tacttgccat accaacaacg ccaaaaattt ccgttgttga acctccaacg acagttgcaa
     8521 taacgtcatc agagagttca atttgaccat ctcttgtatt gatttttaca gtcatatcta
     8581 ctacctcaaa aagtttttat cactctattc tatcatatct cactattatt gtaaaaaaat
     8641 agaggtatct aactatcaaa tcagccttag aaacactaaa aaaagcccta aggctttctc
     8701 agtttattaa acgcgttcta ctttaccaga tttaagcgca cgagctgaaa cccaaacttt
     8761 ttttggttta ccgtcgataa gaacagtaac tttttgaagg tttggtttaa cagtacgttt
     8821 tgtttggttc atcgcgtgtg aacggttgtt accagaaact gttttacgtc ctgtgaaata
     8881 acatacttta gccattttac gagttcctcc tatattgatc aatatttacg gatgtgctag
     8941 caccacatac tagactattc tatcacaact tacttcttct agcaagttct tttcaaaatt
     9001 actttttcat ttgttttatc taaatcgctt acatattttt tataataaga cacacttcct
     9061 tctaattcat tttttagttt acattttctt tcaaaatatt ataatagaca acaataatca
     9121 aaggaggttt catatgacac gtaaaattgg aattataggc atggggcacg ttggatcaac
     9181 tgttgcacac gctcttattg cacaaggtat tggtgatgat tatgtcttta ttgatactaa
     9241 tgttgataag gttaatgctg atatgattga ttttcaagat gctctggcaa atgctactta
     9301 tcacgctaat atagtattaa acgactatga cgcgttgtca gatgctgatg ttgtgatttc
     9361 agccttaggt gatatttcac tacagaataa caaagaagct aatcgctttg ctgaactacc
     9421 atttaccagt aaagaagtct acgacgttag ccaaaagtta aaatcatctg gttttagagg
     9481 gatccttatt gtcgttacta atcctgttga tgccatcacc cagctttatc aagaatatac
     9541 tcaatttcct aaatcacgcg ttatcggaac aggaacctta ttggatactg ctcggatgaa
     9601 aagagtgctt ggtcaacgcc tacaagttga tccaaggagt atcggtggtt ataatctagg
     9661 agagcatggt aactcccaat ttgtcgcttg gagtcaggta actgtaaaag gacaaccagc
     9721 tatatcacta ttaaaagtag aagaattaga acagataaaa gaagcttctt taaaaggcgg
     9781 gcacactgtc ttttttggta aaaaatatac tagttatggt atcgctaccg cagctgtccg
     9841 catcgtttta gctgtgttaa atgattccca tgagttacta cctgtatcta ccttctatga
     9901 gtccgaaggg gtttaccttg gatatccagc tattattggt agagtaggaa ttgtagaaag
     9961 aatttctctt tcactaagcc caaaagaaga gcaacaactc acagattcag cggcatttat
    10021 tcgaaattat cttaagcata tcaatcttta aagagtatct atttaataaa aagtcatata
    10081 atccaaaaaa agctcaccat aagtgagctt ttaactatta tctgttttat gttaattatg
    10141 ctttgtttgc tgaaccaaac acgtcaatac gttcttcaac tgcttctgta attgcatcga
    10201 aaccaggttt caagaattta cgtgggtcaa agagtttttt cttcatgtat tcagcttcgt
    10261 tagcgttgaa ttcaacagcg tagtcacgag ttgcttgaca gaaagctaat tgacattctg
    10321 tgttaacgtt aactttagca acaccaagtt tgatagcttc ttggatttga tcatcaggaa
    10381 tacctgaacc accgtgtaac acgattggga aaccaggaac agcttcagtc aatttcttca
    10441 agtgatcaag gtcaagtcct tcccagtttg ctgggtatgg tccatgaatg ttaccgatac
    10501 ctgcagccaa gaagtcaata ccagtttcaa ccatagcttt agcgtcttcg attggtgcta
    10561 attcaccttt accgacaata ccatcttctt caccaccgat agtaccaact tcagcttcta
    10621 ctgaaatacc tttagcatgt gctttagcaa caacttcgcg tgctttttca aggttttctt
    10681 caactggaag gtgtgaacca tcaaacataa ttgaagtgta acctacttca atacactcaa
    10741 gggcatcatc gtaatgaccg tggtcaaggt ggatagcaac tggtacagta atacccattg
    10801 attcaaccaa agtttcaata agttgtttac aaagtttgta accacccatg tatttagccg
    10861 cacccattga agtttggata agtactggag cttttttagc ttctgctgcg cgcaagatag
    10921 cttgagtcca ctcaaggttg tttgtgttaa atccacctac tgcatagccg ttatcacgag
    10981 ctgcttggac gaatttttct gctgaaacga ttgccattat tcaggcctcc tatttatttt
    11041 ttgggcgttg cccacttaca tgttaattct agcacaattc tataaaaaat gctagttttt
    11101 tactgaaaat acgtgagaaa cttaacagag ttaaacgagg tttttttcag aaagcagtca
    11161 ttccgctttc aattctttac gtgccaatag gatctgtttt ttaacctggt caaaccccgt
    11221 tcctcctaat gaatttcttc gtttaactgc tgtctttgca gttaatatct catagatatc
    11281 gttttctatc aactctgaaa tttcttgata atattttaaa ggtatatcct gtaaataaga
    11341 accattttta ctgcattcta aaaccaattt acctacaatt tcatgtgctt ttctgaaagg
    11401 tatcccctta cttgctaaat aatctgctaa ttctgttgca tttgaaaaat cagtctccgt
    11461 tgaggtcatc attatgtgct cattcacagt cattgtctca agcatattag ccatgatttc
    11521 aatagctatg ctgactgttt ctacgctatc aaacatccct tctttatctt cttgtaaatc
    11581 tttgttataa gctaacggca atgatttcat aacagttagt aaactaaata aatttccata
    11641 aacacggcct gtcttccctc gaattaattc tgccatatca ggatttttct tttgaggcat
    11701 aatagatgat cctgtagaga aagtatctga taatgtgatg aattgatatt cataactgca
    11761 ccaattaatg atttcttcac aaaaacgtga taaatgcatc attaaaatgc tagcattgga
    11821 taaaaattct atgataaagt cacgatctga gacagcatca agcgaattac tatatggttt
    11881 ttcaaaatct aataaacgag ttgtcatatg gcggtcaata ggaaacgttg tccctgctag
    11941 agcagcagct cctaatggtg atagattggt gtgtttcata ttaaactcta atcgctctgt
    12001 atcacgcgta aacatattat agtaagccat tagatgatgt ccaaatgaaa tcggctgagc
    12061 atgttgtaaa tgtgtatacc ctggcataac agtatagata tgattttctg ctaaattaac
    12121 taaggttatt cgtaaatgaa gcaatttctt catcatttct tgcaatttat ctttcaaata
    12181 aaggtgcata tctgtagcca cttgatcatt tcgtgaacgt gcagtatgaa gcttacctgc
    12241 tacttctcca atttcagcgg ttaataagct ctctatgttc atatgaatgt cttcatttga
    12301 tatgctaaaa gtcaattgac ctgctctgta tttatcttgc agtattttta aaccatcttt
    12361 tatcaatcct gcttcttctt gactaataat atccgttttg cctaacattg tgacatgggc
    12421 catagaagcc ttcatatcat agggagctaa tttttggtcg aaactgattg atgcaccgaa
    12481 ctcttcaacc catttttcaa gactactttc aaaacgacca ccccataact tatgattttt
    12541 tgtcatcctc ttatcttcct ttatttatcc tttgtttact tgggcattaa cttgggttgg
    12601 aagtccccag agtttgataa aaccaactgc tgcatcttga tcaaagctat cagcagctgt
    12661 ataggttgct aagttctcat catataatga attagatgag tggcgagcaa cgacttgggc
    12721 agaaccttta tataacttaa cttttgtagt accattaacc actttttgtg tttctttgac
    12781 ataggcaatg atagcttttg tagctggact aaaccataaa gcattataaa taagatttga
    12841 tagttcattt tcaaggatag gtttaaaatg agacacttca cggactagtg ttaaatcttc
    12901 aatttcttta tgggcagcta atagtaccat tgccgcagga cattcataaa tttcacgtga
    12961 tttgataccg actaaacgat tttcaacatg atcaatgcgt ccaataccat gttttcctgc
    13021 aatttcattt agcgataaaa tcaaatctga tagtgtcatt tcttgattgt ttatagctat
    13081 aggcttccca ttttggaacg taatatcaat atattctgca caatcaggag cttcttctgg
    13141 tgattttgta ataccaaaag cttcttctgg cgcttgattc caaggattct caagcacacc
    13201 acattcattg gcacgtcccc ataaattttg gtctatagaa taagggttat ctaaatctgc
    13261 tggaataggt acgccgttag cttttgcaaa agtaatttcc tcttcacgat gccacttcca
    13321 ctcacgaaca ggggcaatta cttttaattc aggatctagt gctgcaattg caacttcaaa
    13381 acgtacttgg tcatttcctt tacctgtaca tccatgtgca attgtggtcg cacctgtttg
    13441 atgtgccatt tcaactaatt tttgagcaat aattggacga ctaagcgctg aaacaagcgg
    13501 gtatttttgt tcatacatcg catgtgcctg taaagctggt aaaacaaaat gctcagcgaa
    13561 ctcatcttta acatctaaaa tataagattc tatggcacct attgttaaag ccttatcgtg
    13621 aataaaatca agatctttac cttcgccaac atccatacag acagcaataa cgtcataatc
    13681 tttttttaac caagctatag caacagaggt atctaaacca ccagaataag ctaaaattaa
    13741 tttttctttt cccataaaaa atccccttcc aaattcctgt attttgtaat attatacact
    13801 tgttatattt attgtcaaat atttttatag tttttgtcta tttttagtat atttatttct
    13861 aaataaataa aacaataact gccaattggc agttattgtt ttaaataatt atgataaggg
    13921 tagttctaca caaaattctg ttccaatttc caactgactg gagacactaa tacgtccctt
    13981 acaaagaaca attatacgtt taactattgc tagtcctaat ccatttcctt gttgattatg
    14041 cgactgatcc gcttggtaaa atttatcaaa tatatgctga acatcatatt gagaaattcc
    14101 cctaccttta tcagaaataa taactctcag ataatgatta ttagtttctt ccattcgtac
    14161 acttaagtct acaatatcat cggaatattt tattgcatta tctaaaaggt ttatccaaac
    14221 ttgcatcagc aagtcagagt ttgagtaaac agtatatggt tttgaatcca gttgaaaatt
    14281 tatcctttta gcttgccaac gttctgttaa actaatcatt gcttgacgaa tttgttcatc
    14341 tacacgaaca gaggatagct ccccaatagt ctcctgatta tccacgcgcg acatttgcaa
    14401 catcgtatca cataatcttg ttaaccttag gctctctgtt ttaacaagac ctaataactc
    14461 actttgtgtt tcttcatcta attctggatc ttgtaatagt tctacaatat tagctactgc
    14521 agcaatcgga gtctttagct catgagaaac attagtgata aagtcttttc gcatataatc
    14581 catatgagat aaagaaacaa tcatctgatt aatactttca gatagctcat ccaattcatt
    14641 ataatactca taggatttac gacgattact gtttcgataa atatgccctt caaagtcacc
    14701 gtctgcaaca ttggacacaa tatggctaat atctaaaatt ggttttgtta aatgataaga
    14761 accgtaccac atactaatac tagctatcac aaggcttaaa aaaacaacag cgagagcgct
    14821 aacttgtaat acatgagtag ttaagactcc ttgaaaaaaa gtaaaaacta ttgctgcaac
    14881 tagtaaaaga gaagctagga ctgagaaaag catcgttaac acaaacatta atgtgaaata
    14941 aaatcttaac ggaaaccgaa atcgccttag cttagtcatt ttttgcacga taccctaccc
    15001 ctctcaccgt ttcaatctta aaatctggta agtgttctac ttttcgtcta attttattga
    15061 tgcaagcatc taccacccgc tcatctaaat cagtatccat tccccaaata ctatccaata
    15121 gttctaaacg tgtgaaaatc ctattaggat agctaagtaa gtggaaaaga accctaaatt
    15181 ctttctgagg aaatacaatt tcttgctcaa aatatttcaa gcttaattca tcttcattaa
    15241 gacaagtatt cccaataatt agctgatgtt gagctacaat attagctctc ctcaagagtg
    15301 ccttaatacg taaagctaat tcttgtaact gcacaggctt aaccatataa tcatctgcac
    15361 caaggccaaa cccttttgtc atatcttcca tttgtgattt tgctgtcata acaatgattg
    15421 ggacattata agcagctgac ctgagttctt gaattaattg ataaccatcc atcatgggca
    15481 tcatgatatc agttataatc aattgtactt cttctgtatc tagacaattt agagcttctt
    15541 gtccatttga agcagtgata aattggtagc cctcttgtgt taattttgtg gtaatgattt
    15601 tttgttgaac aatgttatcc tctacaacta atattttaat cattaaatca cctctagctt
    15661 attgtaacac aagttagaag ttttctttaa ctaatttccc gtcctttaaa cgataaacgg
    15721 tatctactaa atctagaacg cgctcatcgt gtgtcaccat aatagctgca gtattatgtg
    15781 atttaacttc ctgacgaata agttctgtaa cttgacgccc acgttcttca tcaaggctag
    15841 ctgtcggctc atctgccaag ataaccttag gatgattaat aaatgctcta gcaattgctg
    15901 cgcgttgctt ttgtcctccc gataattggt tagggtattt atgtgcacac tgctctattc
    15961 ctaaatctga taataattta ttaatttctt ccaagcttgt tttcttatca tagtgttttg
    16021 caaatcgagc ctcttgttgc aactgttctt ggattgttaa ataaggaagc agttgatgac
    16081 tttgaaatat aaaacctagt gcttcacggc gcaattgtgt tctttgtctt tgagacaaat
    16141 ccgtaacgtt ctcatgattg acatacaagc taccttgatc tgcgccaaga agtaaccctg
    16201 caatggataa aagtgtgctt ttacctgaac ctgagggtcc cagtatagct acaaactcac
    16261 ctgcagaaac tgataaatct aaattatcta atattgataa aagctcatct ccatctggat
    16321 agtgttttga gatgtgtttt aattctaaga ttgctgacat tattctcctc ccccattaat
    16381 aacttcgact gggtcgattt tagcaacttt tacaattgac aaagcactac taatcattgc
    16441 aataactaaa aatacaaacg atactagaat aatattttgc caattaataa caaatggcat
    16501 ttgagctggt aatacataag agagagcaat cgccaatcca tctccaacaa tgataccaaa
    16561 cagtgccaat atgatgactt gactcaattg aaatagggca atctctgaca tttttgtccc
    16621 aatagctttc atgacgctaa attcatgacg tttttgaagt gtgatgatgt agaaaaagac
    16681 acctaaaata ccagctgagg ctacgacaag gacccataaa atcatattca aagtggattg
    16741 ttcggcacta taaccaggaa tatgttgaat gacatctttt ttactaacag cttctaattg
    16801 agttggtaaa tgagatatac ttttatcatt tttcataact aatgcctgag gtaagaactg
    16861 gtattgagga ttaatctttt tattaatttc ggtataaatg tctttatcaa taaatgccac
    16921 aggtccgtga ccatacatac tattatggac aaatccaacg actgttagtg atatagatga
    16981 tgacttatca atcaccttat cacctaactt tatcccttca gcttttaaac tatcatttaa
    17041 aataatagct tttttgtaac ttgtcagttg ttttccctca gaaagtgtag gtgccataaa
    17101 agaatcttta tcaatagcaa aataagaaat atcaattttc ttttcatgtc cttgtcgagt
    17161 taaactcgaa cgttggatat ttaaagtggt actatctttt aaattcaaac tgtttaagtc
    17221 tgtctgatct ttagttgtca atacagatga tgtaatcact tgttcagcac cttcattcaa
    17281 aatataggtt tgagctgggt tattttcaat agcagcacta acagctctac ctaaaccgtt
    17341 tgctaaacct gataaaaaaa ctaccatgaa catcataaca atgattagca attcaatcag
    17401 gaggtatttt ttcttataaa aggttaattc tttccaagca tgtgacatgg tttttctcct
    17461 ttgatctttt gatgtgaaca tagtatcaca gctttatgac ttgaatatga cctctaaaaa
    17521 taatgatatt ttatcattaa attatttaaa aaatagcttg acaatttttt agtaagcgat
    17581 tacaattcta attatgaaac cgttcccaca ataaaagaaa ggagttttta tgagtacgat
    17641 tagacaagtc gccgagaaag ctggcgtatc aacatctact gtctcaaggt atatatccca
    17701 aaatggttac gttagtcaaa aagcaagtca aaaaatcgag caagctatcc gcgaactcca
    17761 ttatgttcca aatttccttg cacaatctct aaaaaccaaa aaaaatcaac ttgttggatt
    17821 actacttccc gatatttcaa accctttctt tccaagactt gctagaggag ttgaagaatt
    17881 tctaaaagaa cagggttatc gtgtcatgct tggcaacaca aataacaaga gccatttaga
    17941 agaagagtac ttaaatgtgc tattacaaag taatgctgca gggattatta caacacacga
    18001 tttcaccaaa aatcatcctg aaatagatat tccagttgta gttgttgacc gcgttaatca
    18061 agaaacacag tacggcgttt tctctgataa taaagaggga ggaaaattag cagctcaagc
    18121 tatttggaca gcaggtgcta ccaatatatt actcattcgt ggtccgctag ataaagctga
    18181 taaccttaac caaaggtttc aaggaagcca aaattaccta ttaaacaaag gggcttgttt
    18241 tgctatcgaa gatagtgcca gttttgattt tgcagagatt caaattgaag cgaaaacttt
    18301 acttgaccac catccagaca ttgatagcat tatcgctcca tctgatatac atgctattgc
    18361 ctacctacac gagattctta atcgagggaa gcgaatccct gaagatgtac aaattattgg
    18421 ttatgatgat attttaatga gccaatttat ctacccttcg ttgtcaacta ttcaccaatc
    18481 gtcatatatt atgggacaaa aagcagctga attaattttc aaaattacta atcaattacc
    18541 tattaccaat aagcgtatta aattacccgt tcattatgtc gaacgtgaaa ccctaaggag
    18601 gaaaatagat gagtaacatt gtcattatcg gtagtatttc catggactta gttatggaaa
    18661 ccaatcgtat cgccaaagag ggagaaaccg tatttggcca acgtttttca atggttccag
    18721 gaggtaaagg tgctaatcag gctgtcgcta ttgggcgtct tagccaagat cgagataata
    18781 ttactattct tggtgccatt ggcgaggatt cttttggtcc cattcttttg gataatttaa
    18841 acaaaaatca tgttactacg gattttgtgg gaacgatacc atcttcttca ggtgttgccc
    18901 aaatcactct ttataataat gataatcgaa tcatttactg tcctggagcc aacggaaaag
    18961 ttgatactaa aaaatggtct caggagtggg caataattaa aaaagctgat ttagttgtat
    19021 tacaaaatga aattcctcat caagcaaata tgaaaattgc taatttttgt aaagaacata
    19081 gtattaaatt actctataat cctgcgccat ctagagagac tgatattgag atgcttgata
    19141 aggtcgatta tttcacacca aatgaacacg aatgtcaaga gctgttccca aaccaaaaac
    19201 tagaggatat attagccaca tatcctgaga aactaattgt aacactcggc acaaaaggag
    19261 ccatctactc tgatggaaaa gaaagccatt taattcctgc cttagaaact aaagctgttg
    19321 atacaactgg tgcaggtgat acttttaacg gtgcattcgg ttatgctatc tcgaaaaaat
    19381 ttaagattgc taatgcctta cgttttgcaa ctctagctgc tcacttatcc gtgcaaaaat
    19441 ttggtgcaca gggtggcatg cctacaatta aagaaatgga ggacaaccaa cattatgaaa
    19501 aagactggca tattaaatag tcatctcgct aaattagcag atgaccttgg gcatactgat
    19561 caagtttgta ttggagatct gggtctacct gttccaaatg gcattccaaa gattgattta
    19621 tctttgacct ctggagtacc tagtttccaa gaggtacttg atatctattt agagaatata
    19681 ttagttgaaa aagtcatttt agcagaagaa atcaaggaag caaatccgga tcaattatca
    19741 aggttattag ctaaacttga taactctgtt agcatagagt acgtcagtca caatcattta
    19801 aaacaaatga cccaagatgt caaagcagtt attcgtacag gtgaaaatac accgtattca
    19861 aatattattc tacaatctgg tgttatcatc tagaaaggag tttagtccat gaaaattgac
    19921 atgaggaata tctccaaatc cttcggtact aataaagttc tagagaagat tgatttggaa
    19981 ttacaatctg gtcaaataca cgcacttatg ggtgaaaatg gtgcgggtaa atcaacttta
    20041 atgaacattc taacaggact ttttccagca agtacaggaa ctatttatat tgatggcgag
    20101 gaacgaactt tttcaaaccc tcaagaagca gaggaatttg gcattagttt tattcaccaa
    20161 gagatgaata cttggccgga aatgactgtc ttggaaaatc tctttcttgg acgtgaaatc
    20221 aagacaactt ttggcttact caatcaaaaa ttaatgagac aaaaagcact tgaagctttt
    20281 aaaagattag gtgtgactat tcccctagac atcccgattg gtaacttgtc tgttggacaa
    20341 caacaaatga ttgaaatcgc caaaagtctc ctaagccaac tctctatttt ggtaatggat
    20401 gaaccgactg ctgctttaac agatcgtgaa actgaaaatc ttttccgagt tatccgaagt
    20461 ttgaaacaag aaggagtggg cattgtctat atctcccatc gaatggagga aatttttaaa
    20521 atcactgact tcgtaaccgt catgagagat ggtgtcattg tagatacaaa agaaacatca
    20581 ctcacaaatt ctgacgaatt ggttaaaaaa atggttggcc gaaaattgga agattactat
    20641 cctgaaaaac attctgaaat tgggcctgtt gcttttgaag ttagtaacct ctgtggagat
    20701 aatttcgagg acgtttcctt ttatgtccgt aaaggcgaga ttcttggatt ttctggatta
    20761 atgggagctg ggcgtactga agttatgcga actatctttg gaattgataa aaagaagtct
    20821 ggtaaggtga aaattgatga ccaagaaatc actattacta gcccaagcca agctatcaag
    20881 cagggcattg gttttttgac cgaaaataga aaagatgaag gtctcatcct agacttcaat
    20941 atcaaggaca atatgactct ccctagcact aaagatttta gcaaacatgg tttctttgat
    21001 gaaaaaacaa gcacaacatt tgtgcaacaa ttgattaatc gtctctacat taaatcaggt
    21061 agaccagatt tagaggttgg aaatctttct ggaggtaacc aacaaaaggt cgttctcgct
    21121 aaatggattg gtattgctcc taaagtctta attcttgatg agccaacgcg tggtgttgat
    21181 gtcggagcta aacgagagat ttaccaatta atgaatgaac tagcagacag aggtgttcca
    21241 attgtcatgg tatcatcaga cttacctgaa attctaggtg ttagtgaccg catcatggta
    21301 atgcacgaag gtcgaatcag tggagaactc agtcgaaaag aagctgacca agaaaaagtt
    21361 atgcaactag ccacaggagg taagtaaagt gaaaaatatg atgaaatata tatcagagtt
    21421 aacaaccctg attgcgttag ctggtttaat ggttattatc acactcatta atcctaattt
    21481 cctcacaacc aataatctac tcaatttatt attacaagta actgcaaatg gttttatcgc
    21541 tttcgggatg accttcgtta ttttgactgg cggtattgat ttatcagtcg ggtccatatt
    21601 agccctatct agtgcattaa cagctggatt aatcggccac ggagtacccg ttggtattgc
    21661 tatcgtttta tctgttgcct taggtggcat cttgggaatg ctaaacggac tcttcatttc
    21721 ttacggaaaa ttagcaccat ttatcgttac actggcaaca atgactatct ttagaggagc
    21781 aacgctagtt tattcaaacg gcaacccaat taccgctggc ttatccgata gttttctatt
    21841 ccaatttttg ggacaaggct atattgttgg tatcccattc ccagtcattc tcatgtttct
    21901 tacttttatt attttgtata ttttgttaca taaaacagcc ttcgggaaat ctgtttacgc
    21961 tttaggaggt aatgaaaaag cagcgtatat ctcgggtatt aagttaaaca aagttaaaat
    22021 tatcatctac actatctcag gtatcatggc ttccatttct gggttaatta tcacatcacg
    22081 ccttagctca gcacaaccaa cagctggagc aagttatgag atggatgcta ttgccgctgt
    22141 tgtcttaggt ggaacgtctt tatctggtgg gaaaggacgt atcattggaa cattgattgg
    22201 tgctcttatt attggtgtcc tgaacaatgg attaaacatc attggtgtct ctgcattttg
    22261 gcaacaagtt gtgaaaggta tcgttatttt aatggctgtt ttacttgatc gctttaaagt
    22321 agcaaaatag tctttactct aatttctata agttcattat attttatcta aaggagtttc
    22381 aaatgaaatt tggaaaaaaa cttggctttc tagccttatt gatgtctatt gtcctcattc
    22441 tcggtgcatg tggtaaaact ggacttggaa attcttctga gaattctaca aaaaatgtaa
    22501 ccaaaaaatc tgctaaagat ttgaaactag gtgtatctat ttcaacaact aacaacccct
    22561 actttgttgc gatgaaagac ggtattgaca aatatgcgag caataaaaaa ataagcatta
    22621 aggtagctga tgctcaagat gacgccgcac gtcaagcaga tgatgtccaa aactttatta
    22681 gtcaaaacgt agatgctatt ttgattaatc ccgttgactc aaaagctatt gtaacagcta
    22741 ttaagtctgc aaacaacgct aatattcctg ttattctaat ggaccgtggt agtgagggag
    22801 gcaaagtatt aactactgtc gcttcagata acgttgctgc tggtaagatg gctgcggatt
    22861 atgctgttaa aaaactaggc aaaaaagcaa aagcctttga attatctgga gtaccaggtg
    22921 cctctgctac tgtagaccgt ggtaaaggat ttcattcagt tgcaaaatct aaacttgaca
    22981 tgctttcaag ccaatctgcc aattttgacc gcgccaaagc tttgaatact acacaaaata
    23041 tgattcaagg acataaagat gtccaaatta ttttcgcgca aaatgatgaa atggcacttg
    23101 gggctgcaca ggctgtcaaa tctgctggct tgcaaaatgt tcttatcgta ggaattgatg
    23161 gtcagccaga tgcccatgat gccattaaaa aaggagatat ttcagccact attgcacaac
    23221 aaccagctaa gatgggagaa atagcaattc aagcagcaat tgatcattat aaaggcaaaa
    23281 aagtagagaa agaaacaatt tctccaattt accttgttac caaagacaat gttgaaaaat
    23341 acaattggta aaagtacaca aaaagtcgtg aaaattcacg actttttata ttataatttt
    23401 gctaacatat tatcgatatg ttgaactgat ttttctttac ctaataaata aatagtatct
    23461 ggtaactctg gtccatgcat ttcaccagaa accgcaatac gaataggcat aaataggttc
    23521 ttacctttaa tacctgtttc tttttgaaca gccttaattt gagggaaaat agtatctcta
    23581 gtgaattctt cgtcagaaag cgatactaac ttttccttaa aaacagataa gactgttggg
    23641 actgtttctg ctgccattac ttctttttcg gcttctgtta attctggaaa atctgcaaag
    23701 aaaaggtctg ttaatggtac aatctcatca gccgatttta attgaggttt ataaagctca
    23761 actaattttt cagctttgtc tgtaagtcga ccagcttctt ctaagaatgg tttgcaaagt
    23821 gcaaaaacac tctcaaaatc agcatttttt aggtaatcat tactcatcca atccattttc
    23881 ttctgatcga atgctgctgg tgatttgcta agacgatttt catcaaatag attaattaat
    23941 tgctcgcgtg agaagatttc ttcttctcca ccaggattcc agccaagaag ggcaatgaaa
    24001 ttaaagacag cttccgacat ataacctttt ttacgatagt cttcgataaa ttgaagagta
    24061 ttggtgtcgc gttttgataa cttcttacca gtctctgagt ttataatcaa tgtcatgtga
    24121 ccaaattgtg gcgcttccca accgagtgct tcataaacca ttaattgttt tggcgtattt
    24181 gcaatatggt catcgccacg gataacatgc gaaatttgca tgtcatgatc atcaatgact
    24241 actgcgaaat tgtaagttgg ataaccatct ttcttctgaa taacccagtc tccgccgata
    24301 ttagagcctt caaactcaat gtcacctttt accatatcag tccatttata aatcccagat
    24361 tcactaacag caaggcgtac tgtcggaatg atgcctgctg cttcacgctc tgcaatgtaa
    24421 gcttcttttt cagtttctga cattccgata aattcgttga tatagcgagg tgtttcgcct
    24481 gccaattctt ggcgttcacg ctctgctgct aattcttcct cagtcacata ggacttgtag
    24541 gctttacctt ctgctaacaa ctgatcaata taacgttggt agagctcaag gcgttctgat
    24601 tgacgataat tttcatgtgt ttcaggactt tcatcccagt ccatacctag ccaacgtaag
    24661 ttctccaatt gagagcgttc accatcttcg acgtggcgtt ttcggtcagt atcttcaata
    24721 cgaataacaa aatctccacc atgatggcga gcataaaggt agttaaagag cgcagtacgc
    24781 gcatttccga tgtgtaacaa tcctgttgga cttggtgcat aacgcacacg aatcttatta
    24841 gccaaaattc tactcctata ttttctgata gtaaactttt tcagtttaac tgaattagtc
    24901 aagggggcaa aacatcattc aaactcttac agatgattgc caatcaaccc tattatacca
    24961 ctaaatcact aaaaatgata atcagtaata atagccacac taattttcga taaa
//
LOCUS       DATRSD010000024        23580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRSD010000024 DATRSD010000000
VERSION     DATRSD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.024"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            146..763
                     /locus_tag="VBD85_000774"
     CDS             146..763
                     /locus_tag="VBD85_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133339.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDGSSNIVLRANNKVKKANILARIQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            931..2370
                     /locus_tag="VBD85_000775"
     CDS             931..2370
                     /locus_tag="VBD85_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7133340.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFNLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            2398..2676
                     /locus_tag="VBD85_000776"
     CDS             2398..2676
                     /locus_tag="VBD85_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133341.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            2743..3228
                     /locus_tag="VBD85_000777"
     CDS             2743..3228
                     /locus_tag="VBD85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7133342.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTESVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            3341..4006
                     /locus_tag="VBD85_000778"
     CDS             3341..4006
                     /locus_tag="VBD85_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO7133343.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            4010..4873
                     /locus_tag="VBD85_000779"
     CDS             4010..4873
                     /locus_tag="VBD85_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO7133344.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            4875..5591
                     /locus_tag="VBD85_000780"
     CDS             4875..5591
                     /locus_tag="VBD85_000780"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO7133345.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIIVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            5602..6249
                     /gene="fsa"
                     /locus_tag="VBD85_000781"
     CDS             5602..6249
                     /gene="fsa"
                     /locus_tag="VBD85_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7133346.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            6413..7396
                     /locus_tag="VBD85_000782"
     CDS             6413..7396
                     /locus_tag="VBD85_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7133347.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            7631..8650
                     /locus_tag="VBD85_000783"
     CDS             7631..8650
                     /locus_tag="VBD85_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133348.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVFKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYMIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            8669..9625
                     /locus_tag="VBD85_000784"
     CDS             8669..9625
                     /locus_tag="VBD85_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7133349.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKSRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLVEEAADNIIAFFDGKPQNIVNK"
     gene            9723..11174
                     /locus_tag="VBD85_000785"
     CDS             9723..11174
                     /locus_tag="VBD85_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7133350.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVVIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            11196..12185
                     /locus_tag="VBD85_000786"
     CDS             11196..12185
                     /locus_tag="VBD85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7133351.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAEAFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            12251..13768
                     /locus_tag="VBD85_000787"
     CDS             12251..13768
                     /locus_tag="VBD85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO7133352.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILAHHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            13788..14549
                     /locus_tag="VBD85_000788"
     CDS             13788..14549
                     /locus_tag="VBD85_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO7133353.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNSLACDTTYLHCKNFLQVAGKPHLSKSLFEGEIDLTDLLK
                     SFSNCEYLALEYPTELEVLKRDVQRLISISNSQNF"
     gene            14615..16294
                     /locus_tag="VBD85_000789"
     CDS             14615..16294
                     /locus_tag="VBD85_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO7133354.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            16611..17702
                     /gene="ulaG"
                     /locus_tag="VBD85_000790"
     CDS             16611..17702
                     /gene="ulaG"
                     /locus_tag="VBD85_000790"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO7133355.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            17792..20170
                     /locus_tag="VBD85_000791"
     CDS             17792..20170
                     /locus_tag="VBD85_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO7133356.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGHEAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            20546..21769
                     /locus_tag="VBD85_000792"
     CDS             20546..21769
                     /locus_tag="VBD85_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7133357.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            21788..23515
                     /locus_tag="VBD85_000793"
     CDS             21788..23515
                     /locus_tag="VBD85_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO7133358.1"
                     /translation="MENLLQHKLPVAPFVESATNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITAAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         7431 a         3884 c         4765 g         7500 t
ORIGIN      
        1 agcttattat atcataatgt ataatcaaac attcgttagt actgattaaa aggtttatga
       61 aggggtggtc actattgttt cattgaatta gtaactttaa cgacgaaatg ttataatgtt
      121 attagtttat aaaagaggag attgtatgcg taaatttttc caagatgtta ttgtagaggt
      181 ggcttattat ttagagattg ctttgtcagt tattctggca acagctatta ttttctttag
      241 tttagcactc tttcatgatt tgtttagcat gttgtctgtt gatcaaaatg ttgataagtt
      301 gttccagact ttccttgctc gcgccatgac aatagcagtc gggattgagt tgatcaaaat
      361 gttttcaaaa ccttccccag atactttaat tgaagtgtta ttgtttgcct tagctcgtca
      421 acttgtaatt gaacatggta gtgtttcaga ttaccttatg gggattgctt cagttgctat
      481 tttgtttgct gttcgtaaat atcttttcac ccattttgat gggtcaagca acattgtttt
      541 acgtgctaat aacaaagtca aaaaagctaa tattttagct aggattcaac taccttgtca
      601 acccaatgaa actttaggag acttacttgt tcgtcattta aatgatgagg aaaaggacgt
      661 tgctattggt tcagtagtct atattaataa cgttgcattg cgtgttgata gtatgcacga
      721 tggtcttgtg acgcgtgtag aaatcattag gagtctacat taaaaaatat aaaaaatcat
      781 gtatcttagg atacatgatt tttttgtaaa ttaggttcag cacaattttg cacacttaag
      841 tgtgtacttc ttggtctttt ttaaaacgct tacaaaaaat ataataaaga tacaaaaaaa
      901 cattacttaa ataaagagag gtctttagtt atggaaaact tcctagctcc ccttaattgg
      961 ttctcgcaaa atatcttgca gaatccagcc ttcttcgtag gtctattggt actcattggg
     1021 tatcttttat tgaagaaacc acttcatgat gtctttgctg gttttatcaa agcgaccgtt
     1081 ggatatttga ttttgaacgt aggtgcagga ggattggtta atacattccg tccaatctta
     1141 gttgctcttg ctaaaaaatt taatttagaa gctgctgtta ttgaccctta ctttggttta
     1201 gcatcagcaa atgctaagtt agaaacaatg ggctttatca gtgttgcaac aacagcactc
     1261 ttgattggtt ttgggattaa cattctcttg gttgctcttc gtaaagtaac taaagtccgt
     1321 actcttttta ttactggtca tatcatggta caacaagctg caacaatttc agtatttgta
     1381 ctgctcttga ttccacaact tcgtaatggt tttggtgcat gggctgttgg tatcatttgt
     1441 ggtctatatt gggcggtaag ttctaatatg accgttgaag caactcaacg ccttacaggt
     1501 ggtggtggat ttgccattgg acaccaacaa caatttgcaa tttggtttgt tgataaggtt
     1561 gcgccattct tcggtaaaaa agaagaaaac cttgataact taaaattacc aacattctta
     1621 aatattttcc atgatacagt tgttgcatca gcaacattga tgctagtttt ctttggagga
     1681 atcttagctg tccttggtcc agacattatg tctaatgtta aattaattgg acctggggca
     1741 tttgtaccaa ctaagcaagc tttcttcatg tacatccttc aaacatcatt gacattctca
     1801 gtttaccttt tcattttgat gcaaggtgta cgcatgttcg ttacagaatt gacaaatgcc
     1861 ttccaaggca tctcaaacaa acttcttcca ggatcattcc cagcggttga cgttgcagct
     1921 tcatatggtt ttggttcttc aaatgcagtt ctttcaggat ttgcttttgg tttgattggt
     1981 caattaatta caatcgctct acttgttgtc ttcaaaaatc caatcttgat tatcacagga
     2041 ttcgttccag tattctttga taatgcggca atcgctgttt atgcagataa acgtggtggc
     2101 tggaaagcag cagtagctct atcatttatc tcaggtatta ttcaggtggc acttggtgct
     2161 gttgctgtag gtcttttagg acttgcaggt ggttatcatg gaaatattga ctttgaattc
     2221 ccatggttag catttggtta catcttcaag tatttaggta tcgctggtta cgtgattgta
     2281 tgtctcttct tcttagctat tccacaactt caatttatga aatctaaaga taaagaagca
     2341 tactatcgtg gtgatgcgcc tgcagaataa tagaaattta taacggagga aataaaaatg
     2401 gttaaagttc ttacagcatg cggaaacggc atgggatcat caatggttat caaaatgaaa
     2461 gttgaaaatg cgcttcgtca attaggcgtt agcaactttg agtcagcatc ttgctcagtc
     2521 ggagaagcaa aaggtttagc tgcgaactac gatatcgtgg ttgcttcaaa ccaccttatc
     2581 catgaattag acggacgtac aaaaggtcat ttagtaggtc ttgataacct tatggatgac
     2641 aatgaaataa aaacaaaact acaagaaatt ttgtaagtta atagacaaag ggagtgagac
     2701 aaaagtttct ctcccatatc tttagacttt taaggagaaa tcatgaattt aaaacaagcc
     2761 tttatcgaaa atgactcaat tcgtcttaaa ttatctgcaa gcgattggaa agaagcaatc
     2821 aaattatcaa ttgatccatt gattgaaagt ggtgctgttg atgcagaata ttacgatgcc
     2881 attattgaat ctacagagga atttggtccg tactacattc ttatgccggg tatggcgatg
     2941 cctcatgctc gtccagaagc aggcgtaaaa cgtgatgcat tttcattaat tacattaaca
     3001 gagtcagtag tctttccaga tggcaaagaa gtctcagtat tgttagcact tgctgcaaca
     3061 agttcagcta ttcatacttc tgttgctatc cctcaaatta ttgccctttt tgaattggaa
     3121 aattcaattc agcgcttgac ggaatgccaa gaagcaaaag aagtccttgc aatggtagag
     3181 gaatcaaaaa acagcccata tttagaaggt ttagatttag aaagctaatc aataaactga
     3241 tattaataga aaggatagga cctataaaaa atctctgtga taagaaactc tttgccacaa
     3301 ataaaatgag tatttttatc aggtccttag aataaacaac atgacaaaac gtttaccaaa
     3361 tttacaagtt gcacttgacc actcagattt acaaggagct atcaaagctg ccgtatcagt
     3421 aggacatgaa gttgatgtta ttgaagctgg aactgtttgc cttcttcaag taggtagtga
     3481 attagttgag gtccttcgca gtctcttccc tgacaaaata attgttgctg atacaaaatg
     3541 tgctgatgct ggtgggactg ttgctaaaaa taatgctgtt cgcggtgcag actggatgac
     3601 ttgtatctgt tgtgctacta ttccaacaat ggaagcagcc ttaaaagcta tcaaagaaga
     3661 acgcggcgat cgtggagaaa ttcaaatcga actatatggt gattggactt atgagcaagc
     3721 gcaacaatgg ttggatgcag gtatttcaca agccatttat caccaatctc gtgatgcgct
     3781 tcttgctggc gaaacatggg gagaaaaaga tcttaataag gttaaaaaat tgattgatat
     3841 gggcttccga gtttctgtaa ctggtggttt atcaactgat actcttcaat tatttgaagg
     3901 tgttgatgtt tttactttta ttgcgggtcg tggtattaca gaggcagatg atccagccgc
     3961 agcagcacgt gcatttaagg atgaaattaa acgtatttgg gggtaatgta tgacacgtcc
     4021 aataggaatt tacgagaagg caacaccaaa acattttaac tggttagaac gcctacaatt
     4081 tgctaaagaa ttgggatttg attttgtaga attatccatt gatgagtcag atgaacgttt
     4141 ggcacgtttg gagtggtcaa aagaagaacg acttgagttg gtcaaagcta tttttgagac
     4201 cggagttcgt gttccaacaa ttacatttag tggtcatcgt cgtttcccaa tgggatctaa
     4261 tgatcctgag aaagaagcgc gtgcgatgga tatgatgaaa aaatgtatcg tctttgctca
     4321 agatattggg attcgtaata ttcaattagc aggatatgat gtgtactatg aagaaaaatc
     4381 tccagagaca cgtgcacgct ttattaagaa tcttcgtcaa gcttgtactt gggccgaaga
     4441 agcacaagtt attctgtcta ttgagattat ggatgaccca tttatgaatt ccattgaaaa
     4501 atatttggct gtggaaaaag agattgattc tccttatctc tttgtctacc cagatactgg
     4561 aaatgtatca gcatggcata atgatctttg gagtgaattt tataatggac atcgctcaat
     4621 tgcagcgctt catattaaag atacctatgc tgttaccgaa acatctaaag gtcaattccg
     4681 tgatgttcca tttggtcaag ggtgtgttga ttgggaagaa atgtttgctg tcattaagaa
     4741 aacaaattat aatggtccat tcttgattga aatgtggtcg gagaactgtg agactgtaga
     4801 ggaaacacgt gcagccatta aagaggcgca agattttctt tacccattaa tggagaaagc
     4861 aggagtgaaa taatatggca aaatcattgc aagaaatgcg tgaacgtgtg tgtgaagcta
     4921 ataaatcact accagttcat ggtttagtta aatttacttg gggtaacgtt tctgaagttg
     4981 accgcgaagc aggattgatt gttatcaaac cttcaggagt tgactatgac caattgacac
     5041 ctgaaaatat ggttgtcaca gacttagagg gaaatatagt ggagggggat ttgaatccat
     5101 catctgatct tccaactcat gttcaacttt ataaagcatg gccagaagtc ggtggtattg
     5161 ttcatactca ttcaacagaa gcagttggct gggcacaagc gggtcgcgat attccatttt
     5221 atggaacaac acatgcagat tatttttatg ggccagtacc atgtgctagg tcacttagtg
     5281 aagatgaagt taacactgct tatgaaaaag aaacaggtag tgttattata gaggaatttg
     5341 aacgtcggaa tttagatcca atggctgttc ctggtattat tgtacgtaat catggaccat
     5401 ttacttgggg gaaagatcca gcacaagcag tttatcactc agtcgttctt gaagaagttg
     5461 ccaagatgaa tcgtttcact gaacaaatta atccacgtgt tgaaccagct ccaaaatata
     5521 ttatggataa acactattta cgtaagcatg gaccaaatgc ttattatggt caaaaaggac
     5581 aagaacatta aggagaataa tatgaaatac tttttagata ctgccgatgt ttctgaaatt
     5641 cgtcgtctta atcgtttagg aatcgttgat ggtgttacta ccaatccgac tattatttca
     5701 cgtgaggggc gtgactttaa agaagttatt aacgaaattt gccaaatcgt tgatggtcca
     5761 gttagtgcag aagtgacagg tttaacttgt gatgaaatgg ttaccgaagc tagagaaatc
     5821 gctaagtggt caccaaatgt tgttgttaaa attccaatga ctgaagaagg tttagcagca
     5881 gtttcacaat tatcaaaaga aggtataaag acaaatgtga cattaatatt tactgtggct
     5941 caaggtcttt cggcaatgaa agctggtgca acctttatta gtccttttgt gggtcgtctt
     6001 gaagatattg gaacagatgc ttatgctttg attcgtgacc ttcgtcacat tattgatttt
     6061 tatggtttcc aatctgagat tattgctgct agcattcgag gattggcgca tgtggagggc
     6121 gtcgctaaat gcggtgcgca cattgccact attccagata aaacatttgc atcactattt
     6181 actcacccgc ttactgataa agggattgaa actttcttaa aagattggga tagtttcaag
     6241 aagaagtagt gttacgataa agcaaactct taattatact agtgcaagac aaactttgat
     6301 aatgggtttg tcttttttta taatcttctc caataagaac acttattttc atttttctta
     6361 ttaaatgata taatacgggt aaattaagtc taattagaat aaggaaatag agatgtttga
     6421 gaaggtaacc attagtgata ttgcaaattt agtaggtgtg tcaaaagcta cagtttccta
     6481 ttatctcaat gggaactata agaaaatgtc tctgcaaacg aaagaaaaaa tacgtttagc
     6541 tattaaggag actggttatc aaccaagtaa gattgctcaa agtttagtta caaaaaatac
     6601 ccgtactatt ggggtcgtta ttgctgatat tactaatccc tttatttcgt ctgtgatgaa
     6661 gggaatacat gatacttgtc aacaatttgg ttactcggtt aactttacta actcggataa
     6721 tgatattgac attgagttgg aaaatttaaa ccgtttaaat cagcaaaacg tttcaggaat
     6781 tattttagat tctgtagatc ctaatcattc atttatagaa actttgagta atgatcgtct
     6841 tgtcatggtt gatagacaag ctaaagatat taaggtagat actgtagtat ccgacaataa
     6901 agaatcgact caaatatttc ttgaaaaaat gcaagaaact gggtatcatg acatctattt
     6961 tgtcacttat cccattgaag gaatttcaac ccgtgagctt cgttacgaag gttttaaaga
     7021 agtggtcagt tcaaatcctg ataagttgat tattatcact gaagatggta gtacacagag
     7081 aattcttgat attattgaac attcagagca aaaaccaggg ttcttaatga tgaacggtcc
     7141 aactcttttg aattttatga aaaaattaaa tcaatcaacg gtcagctacc ctgaagatta
     7201 tggtcttggg agttatgaag atttagaatg gatgcaggta cttacaccaa acgtttcctg
     7261 tataaaacag gattcatatg gcattggttg cctggctgcc caatgtttaa ttgaaaaaat
     7321 tagtcaagga aacgagccaa ctactgcaag gttactagaa gtaaaaaatc aaatcgttat
     7381 tcgtcaatct ttttaaaaat gtttgcgaga gcaagcattt ttttataaaa aagcaaaaaa
     7441 cactatgtaa acgcttgctt ttataaaaaa tgatagtata atagaattat aaatgagtta
     7501 accgtttaac ttaagaatag gtaagctttt aacgaaccat ctctaactct cttaactcaa
     7561 atgtttttat ttttttgcac cattagttaa acggttaact caagttttat cttcaccaaa
     7621 ggaggataga atgaagatta ataaattatt gtcagacaga cttgaagctt gttattcagt
     7681 ttgtcgtttg caaaatcaag actatgattt tttattagta gcttcagagg ttgatcaagc
     7741 ctgctttgct tatgatttga atagcgattt agcaaaaatt gttgtttggg aagatgtggg
     7801 aggtaccatg agtattattc caattcccgg aaccatggat tttttggcaa cccagaaatt
     7861 ttacccaggg tttaaggcca aagattgtca ggttgtttat gcaagttttc aaggagatca
     7921 ttgggacatt tgtaacgcat ttgattttcc gtatttacat cgctttgatc tcattaaagg
     7981 taaagaaaat caactttact ttataggttg tacaattgct aacagtaaag cctacactga
     8041 ggattggagt gataaaggcc gaatttttgt tgctcgtttt aatactcaaa accatacatt
     8101 ggaaggattg caacaattgc ctcaaacttt attaaaaaat catggatact atgccattca
     8161 ggatgaagga tattcattga ttacttcagt ggaaggggta ttcaaactca cttatccaga
     8221 attttctact acaggcgact ggcaattaga acggcttttc gatgaggaga caagcgatgt
     8281 ggtgaaagtg gatattaatc aggatggcaa ggatgagtat atgattatcc aaggttttca
     8341 tggagatcgt ttacgtatct tcactgaaga tttcggtcga gaattattcc attatccaga
     8401 aaaaacccca tttggtcacg ctatttggag tggtcgttta cttaatcaga cttgttttgt
     8461 attcgggtgg cgatcagaaa aagcagaatt aaggcttttt cactttgtag atgggcactt
     8521 ggtttcagaa ttagtagatg caaaagcagc ttctagtaat gtcttagctt ttgaaaaaga
     8581 tggaaaagct tatcttttct cagccaataa cggacgtggc gaagttgctc tttatcaatt
     8641 agtaaaataa atttaagaag gagacaatat ggacaagaaa aaaatcttag taacgggtat
     8701 tgtgcctaaa gaaggtctaa gaaagcttat ggaccgattt gatgttactt attcagaaga
     8761 tcgcccattt tcacgtgact atgtgttaga gcatttatct gaatatgacg gatggttact
     8821 catgggacaa aaaggcgata aagagatgat tgatgcaggt gaaaacttac aaattatttc
     8881 tttaaatgcg gttggttttg atcatgtaga tactgcgtat gctaaagaaa aaggaattgt
     8941 agtctctaat tcccctcaag cagtacgagt tccgacagcg gaaatgactt ttgctttaat
     9001 tttagctgcc agtaagcgcc ttgcatttta tgatagtatt gtaagaagtg gagagtggat
     9061 tgatccgagc gaacaacgtt atcaaggttt gactttgcaa ggttcaacat taggtattta
     9121 cggaatggga cgcatcggtt tgacagtcgc aaattttgct aaagcatttg ggatgactgt
     9181 tgtttacaat gatgtttatc gacttcctga agataaggaa aaagagcttg gtgtaaccta
     9241 tctcgaattt gatcagctta ttgaaacagc tgatgtcata accatccacg ccccagcatt
     9301 accatcaaca atccacaaat tcaataaaga tgtttttgct aagatgaaaa gtcgaagcta
     9361 cttaatcaac gctgctagag gcccaatcgt ttctgaagaa gcacttattg aagctctgaa
     9421 agaaggtgaa atcgcgggtg cgggattaga tgtttttgag aatgaaccgc aggtatctga
     9481 aggattgaga agtcttgata atgttatcat gagtccacat gctgggacag gtactattga
     9541 gggacgtaga actttggttg aagaagcagc tgacaacatc attgccttct ttgatggcaa
     9601 gccacaaaat attgtcaata aataattggc tagtgagcgc agagccatct tttgaaaaaa
     9661 tgggacctcc cattttttta cgggtaaatg taagcgctta ataatttaat aaaggtgaac
     9721 ttatgctaga agctttacat aaattctttg aagtttttgg tgcagcaact gttgtacccg
     9781 ctatgatttt tattgtcagc ctttttctaa aggtcaatcc aaggcgagct ttctttagcg
     9841 ccttacgagc tggtgttgga ctcacaggct ttggttggat tatatcagct tttgctccta
     9901 tggtgactaa gtatatccag cagatggtta aaactactgg gctccattta ccaattgtag
     9961 atattggttg gcaggctggt tctctcaccg ccttttcttc tgagattggc ctctctttct
    10021 ttgtttttgg cttgctaatt gaattggggc ttttcttgct cgggattacc cgagtatttg
    10081 tcccatctaa tctttggaat aactttggct atatgatttg gggtacaatg gcctatgcag
    10141 caacaggtaa ttttatattg tctttcgcat ttatggtttt tgtcttactc tattcattag
    10201 tgatgtcaga agtattggca gatcgatggt cggagtatta cggtgttaaa aatgcaacga
    10261 tcaactcgat tcataatatt gaaaccctta ttccagctct tattcttgat cctctttgga
    10321 acctacttgg tgtcaataag gtaaagttga acccagaatc attaaagact aagttaggga
    10381 tatttggtga gccaatgaca cttggtttta tacttggtgt gattattggt gtattaggta
    10441 gtttacgtaa ccttgcaagt attgatactt ggggaggtat acttggtttt gcagttgctc
    10501 ttgcagcggt tatgactatt tttcctttga ttacgggggt atttgctagt gcttttgctc
    10561 ctcttgctga agcagttgaa cgcaataaga aaaaagaaag tcaagctgag caaggagcac
    10621 tggataaaaa acgttggttt attgcagtag atgacggtgt tggttttggt gaaccagcaa
    10681 ctattatcgc aggtctcatt ttagtaccaa taatggttgt tatttccttg attttacctg
    10741 gaaatgaagc tttaccagtc gttgacctta ttgctatccc ttttatgatt gaagctatga
    10801 tagctgtgtc aaaaggaaat attcttaaag ctattttgaa tggaatcata tggttttcac
    10861 taggtcttta tgctgctagt gccctaggtc caatctacac agaagctgtt aagcattttg
    10921 gaactgcttt accagcaggg gtaactttga ttatgagctt taatctccta gctcatcctt
    10981 tgacagctct agtcttcttt gcttggatat ctgggaaccc actctatatt ggtgtgacag
    11041 ttgttattta tcttgctttc ttgttcttgc ttcgtactcg tcgtgaatct atttacgcat
    11101 accttaaaac tatggctgat aagaatagcg gatatgaagg tagttgtgaa aaaatcttta
    11161 ttgctgaaga ttaaaaatct gttggaggaa tactcatgaa aaaaattgat ggacatgctc
    11221 atattgttga tacaattgca ggatttaatg gtaaaggacg ccttaatgcc cttgggaacg
    11281 gttatgctat ttgggatgat ggtgatttaa ttaagctcat cccagacgaa tacggtgaca
    11341 aagcatttac agctgaagct tttttgaagt atatggatgc taatggtgta gataaagcag
    11401 tcattttgca aggtcatctt aatggctatc aaaattatta tacgcattta gctattaaac
    11461 gttatccaga acgttttaca ggcgcattct ctgttgatcc atttgcagat aatgcaatgc
    11521 aaattgtaaa acgtcatgtt gaagtccttg gttttcgagc tattaaattt gaaattagtc
    11581 aaggaggggg tatccatgga taccgtggac aaaaaacgcc tttccgtctt gatacagacc
    11641 cacatgtgag tcgtattttg acttatctgt tagactatcc aggatttgta gttacagttg
    11701 attatggcaa ttgggatcaa attagtcacc aacctgatgc tattgctaat ttagctcgcc
    11761 tttatacgag tctcgatttt gttgtttgtc acctctcttt tccacatgtt gagcattcaa
    11821 atcgattgcg tgctgaactt aatatgtgga aaaacttcaa caatatctat actgatattt
    11881 cagctattca agatattgat gccccagata gttttccttt tccaaaatca gaacaaaatg
    11941 tacgtattgc taaagaagtt cttggggcta agcgtatcat ttggggaact gattctccat
    12001 ggtcagcgac cttcaatact tacgaagaat tagctacttg gctagagaat gttgatatct
    12061 tcagtcaaga ggaactagaa gatgttatgt acaataatgc tgagcgtgtt tactttaaag
    12121 aagaacatgt aaaggctaac agagcaggta tagatgatac aaccaaagag ctcaatctct
    12181 attagaagag ttttcaaggt acaagagttt cttgtacctt ctttttaaat caacaatgag
    12241 gagacaaatt atgacttact accttagcat tgactacggc ggtacaaata ccaaggcgct
    12301 tatttttgac aaattaggac accaaatcgc tgtttcgagt tttgaaactt taaaaaatga
    12361 ggctcaatct ggtcatcgtc aagtaaacct tgttaaaacg tggaatgcta taacttctgc
    12421 tattagagag gttattcaaa tctcaaaact cagccctgag cagattagtg cagtagcatg
    12481 tattggacat gggaaaggtc tttatctgct agataataag ttggagccac ttgaacaagg
    12541 aattttgtct acagataatc gtgccaaaga tttggcgcaa tatttcgaat ctaaacttga
    12601 taatatttgg gagttgactc ggcaacatat tttcccttca caaagtccag ttattttacg
    12661 ttggcttaaa gattatcagc ccgaaaccta taaatcaata ggggcggtcc tttctgcaaa
    12721 ggactttatt cggtataagc ttacaggaaa agtacagcaa gaatatggtg acgcttcagg
    12781 taatcattgg ataaatttcc aaacaggaac ttatgatcca gctattttag atttttttgg
    12841 cattagagag atagaaaact cacttcctga acttatagat agtgcagatt tagttcctgg
    12901 gggaattagt tctcaagcag caaaagagac tggtcttgta gaagggaccc ccgttgttgg
    12961 agggctcttt gacatcgatg cttgtgctct tggatcaggt gttttagagt cagatacttt
    13021 tagtgttatt tcgggaactt ggaatattaa tacttatcca agtttaaaac cagcaaagca
    13081 agatagtggt cttatgactt cctattttcc agatcgtcgt tatctcttag aggcaagtag
    13141 ccctacttct gcagggaatc ttgattttat gttaaaaatg ctcatgcatc aagaaattga
    13201 taacgctaaa tctagtggag gttctatcta tgataattta gaagaatttc tcactcatac
    13261 tgatgctaca catcatggac ttattttctt tccgtttctt tacggtagta acacatcaca
    13321 agatgctagc gcttgctttt ttgggctaac aactaaatcg acgaaatctc agatgatacg
    13381 tgcggtatat gaaggtattg cgtttgcaca taagcagcat atcactgatt taataaaaag
    13441 taggggcggt gtgccaaaaa taattcgttt ctctggcgga gctaccaact caccagcatg
    13501 gatgcaaatg ttttctgata tcttaaacct tcctattgaa acagtagaag gcacagaatt
    13561 aggaggatta ggaggagcta ttttagcaca tcatgcttta gataagattt cgttaaagga
    13621 agcagtccaa gatatggttc gtgtaaaagc tatttataaa cctcaattat ccgaagtaaa
    13681 ggggtacgaa aaaaaatatc acgcttacca aaaattatta gaaacactgg atcctatttg
    13741 gtcggaactc ggtcatctga ataagtaggg atatcaaagg agttgttatg aaacaagaag
    13801 atattattat caatagtatt gtattcaaaa aacagttaga caaaggtttt acacaagttc
    13861 agctcattaa gttagtaaag gatttaggat tttcacgatt tgagatacgt caagaattgc
    13921 ttcaagatcc agatagagag ctacccgctt taaaagcaga ggctgacttt tatgatatca
    13981 atctttacta tagtgctaat gaagacctta tcaaaggagg gaaagttaat ccttatctta
    14041 ataaaggcct taaagaagca tcacagttag gagctccttt cattaagcta aatgttggac
    14101 aaaccagaaa cttaagtaaa gaagagttag aacctcttaa agagatattg aaaagccaaa
    14161 ctattggtat aaaggtggaa aataatcaag accctaaagc agctacagtg gagaattgcc
    14221 aatattttat gactctagtg aaagaattac aaataccaat aagttttgtt tttgatactg
    14281 ctaattgggc ctttatcaac caagatctat accaagcggt aaatagctta gcttgtgata
    14341 ccacctacct tcactgtaaa aattttttac aggtagctgg aaagcctcac ctctcgaaga
    14401 gcctcttcga aggagagatt gatctgactg atttgttaaa gagtttctct aattgtgagt
    14461 atctagctct agaatacccc acagagttgg aggtattaaa aagggatgtt caacgtttaa
    14521 tctctatcag caatagtcaa aacttttaat gaaaaggctt taaaaagatg ttataataag
    14581 tctgtatgaa ttcgagtaga aaagaggtat attcttgatt attctcgata aaaaaagtta
    14641 tgacctcctc ttttacctat tgaaattaga ggaacctgaa acagttatgg caattgccaa
    14701 cgcacttaat cagtctaggc gtaaagtgta ttatcactta gagaagataa acgatgcact
    14761 gcctagcgat gtgcctcaga ttgttagtta tccacgtgta ggaatcttgc taacagaaaa
    14821 acaaaaagca gcctgtcgtc ttttattaga tgaagtgact gattatagtt acgtcatgaa
    14881 aagtagtgag aggctgcagc tgtctttagt atctatcgtg gtagctaagg accgcataac
    14941 gattgatagg ttgatgcaac taaacgatgt ttctcgaaat actatcttaa acgatttaaa
    15001 cgaattaaga agtgagttag cagagaaaga atataattta cagttacaat caacaaaatg
    15061 tcgtggttat tttttagatg gtcacccatt gtccattatc cagtacttat ataagctctt
    15121 agatgatatc taccataatg gaagtagtag ttttatagac ctttttaatc ataaactgtc
    15181 tcaagctttt ggtgccagca catatttttc taaagaggtt cttgattatt ttcaccatta
    15241 tctcttcatt tctcaacgaa gtctaggtaa gaaaatcaac agtcaagatg gtcagtttat
    15301 gattcagatt ttgcctttta tactaatggc ttatcgtaag atgcgattaa gtcctgaagt
    15361 acagacctct cttaatagtg attttagctt ggtttggcaa cgtaaggaat atgagattgc
    15421 taaagagttg gctgatgagc tggaagaaaa ttttcagtta tcactggatg agattgaagt
    15481 gggactagta gccatgctta tgcttagttt ccgcaaggac cgtgacaatc atttagagag
    15541 tcaggattat gatgatatgc gagctactct aaccagtttt ttgaaagaat tggaagaacg
    15601 atatcacctt cactttgttc ataaaaagga cttactaaga caacttctta ctcactgcaa
    15661 ggcactctta tatcgtaaac gttatggtat tttttctgtt aatcctttaa cagagcatat
    15721 taaagacaaa tatgaagaac tttttgccat aacctcgtct tctgtaaagc ttttagagaa
    15781 agcttggcaa atcaaattga ccgatgatga tgtagcatat ctaacgattc atttaggagg
    15841 ggaacttcgt aatagtcaac aatctcctaa taaacttaag ttagttattg tatctgatga
    15901 aggaatagcg attcagaaac ttcttttaaa gcaatgtcaa cgctacttaa caaacagtga
    15961 tatagaagct gtttttacaa ccgaacagta tcaaagtgtg agtgatctta tgcatgtaga
    16021 tatggttgtc tctactagtg acgctttaga atctcgtttt ccgatgttag tagttcaccc
    16081 tgttttgaca gatgatgata ttattcgctt gattcgcttt tctaaaaaag gtaactgtgc
    16141 aaatagtaat caatttacca atgaacttga aaaaacgatt gctcaatatg tcaaggaaga
    16201 tagtgaacgc tacgtgctga aatctaagat tgagaaactt attcatcaag aattgctcca
    16261 agacgtcctt ccccttcaaa gtacagtttg ttaaaatata attttatggt agcctctcag
    16321 cttttctgaa gacagaaaaa gaagctgggg cttttttggt gcttttaaac aatgtgctag
    16381 atatttaatc gcaatgagtt cctctatgac atgataatta aagttgagaa agctagtact
    16441 tttaatggga gtacttttta tttgtgctta ttttaattaa aattatgagt ctaatttgga
    16501 acagacagcc cagttttgaa cacataaatg tgtgacactt ggtctttttt tcaaaagtat
    16561 ttgaacttat actagtagat gtaagaaaca attaatcagg aggaaataaa atgcctaacg
    16621 ttaaagatat tactagagaa tcatggattt tatcaacatt cccagaatgg ggaacatggc
    16681 taaacgaaga aattgaagaa gaagttgttg ctgagggcaa ctttgcaatg tggtggttag
    16741 gaaactgtgg tgtttggatt aaaacaccag gtggtgctaa tgttgttatg gacctatggt
    16801 caaaccgcgg taaatcaaca aaaaaagtaa aagatatggt tcgtggacac caaatggcta
    16861 atatggcagg tgttcgtaaa ctccaaccaa acttacgcgc acaaccaatg gttattgatc
    16921 catttgctat taatgaattg gattactatc ttgtttcaca tttccacagt gaccacattg
    16981 atatcaacac tgcggcagcc attattaata atcctaactt agaccatgtt aaatttgttg
    17041 ggccatatga atgtggtgaa atttggaaaa aatggggagt tccagaagaa cgaatcatcg
    17101 tgattaaacc aggtgaatca tttgagttta aagacatcaa agttactgct gttgaatcat
    17161 ttgaccgcac ttgcttagtg acacttccag tagatggtgc agaagaacat gatggagaac
    17221 ttgctggttt agccgtgact gatgaagaaa tggctcgtaa agcagttaac tatatttttg
    17281 aaacaccagg tggaacaatc tatcatggag ctgactcaca tttttcaaat tattttgcta
    17341 aacatggtaa agactacaag attgacgtag ctattaacaa ctatggtgat aacccagttg
    17401 gtattcagga taaaatgact tcaatcgatc ttcttcgtat ggcagaaaat ctccgtgcca
    17461 aagtcattat tcctgtacac tacgatatct ggtctaactt catggcttct acagacgaaa
    17521 tcttacaatt gtggaaaatg cgtaaagaac gccttcaata tgacttccat ccatttattt
    17581 gggaagttgg aggtaaatac acttatccac aagataaaga tagaattgaa taccatcacc
    17641 cacgtggttt tgatgattgc ttcgaacaag aatcaaatat tcaatttaaa gcacttctat
    17701 aatggctaat gagagactgt atgtcgcatg atatgcagtt tttcaatttt aaaatatgtt
    17761 actaaaatca caattaagat taggagaaat aatgtcagaa tttgatacaa aatcatattt
    17821 agaaaaactt gatgcatggt ggagagcagc taattatatt tctgcagcac aaatgtatct
    17881 aaaggataac cctctcttga gacgagagct ggttgaaaac gacttaaagg ttcatccaat
    17941 tggtcactgg ggcactgtac ctggacaaaa ttttatctat gctcacttaa atcgcgctat
    18001 taataaatac gatttagaca tgttctatat tgaaggtcca ggtcatggtg gtcaagttat
    18061 ggtatctaat tcatatttag atggttcata tactgaatta aaccccaata ttgagcaaac
    18121 agaggatggc tttaagcagt tatgtaaaat cttctctttc ccaggtggaa ttgcatccca
    18181 tgcagcacca gaaacaccag ggtcaattca tgaaggcggt gaacttggtt acgcactttc
    18241 tcatgcgaca ggtgccatcc tggacaatcc agatgttatt gctgcgactg ttattggtga
    18301 tggtgaaggt gaaacaggac cacttatggc tggttggcta tctaatacct ttatcaatcc
    18361 agtaaatgat ggtgctgttc taccaatctt ctatttaaat ggtggtaaaa tccataaccc
    18421 aactatcttt gaacgtaaga cagatgaaga attatctcag ttttttgaag gattaggttg
    18481 gaaacctatt tttgcagatg ttgttgaact ctctgaggat catgcggctg ctcatgcttt
    18541 gtttgcagaa aaattagatc aagctattca agagattaaa accattcaat cagaagcacg
    18601 acaaaaacca gcagaagaag ctatccaagc aaaattccct gtcttagttg cacgtattcc
    18661 taagggttgg actggtccaa aagcttggga aggaacacca attgaaggtg gcttccgcgc
    18721 tcaccaagta ccaattccag tagatgccca ccatatggaa catgttgatt ctcttttgtc
    18781 atggcttcaa tcataccgtc cagaagaatt atttgatgaa aatggtaaaa tcgttgatga
    18841 gattgctgct atttcaccaa aaggtgatcg tcgcatgtcc atgaatccaa taaccaatgc
    18901 tggtattgtt aaagcaatgg atacagcaga ttggaagaaa tttgctcttg atattaatgt
    18961 tccaggtcaa attatggcac aagatatgat tgaatttgga aaatatgcag cagatttagt
    19021 agatgctaac ccagataatt tccgtatttt tggtccagat gaaacgaaat caaatcgtct
    19081 tcaagaagtg tttacacgta ctagccgtca atggcttggt cgtcgtaaac cagattatga
    19141 cgaagcttta agtccagctg gacgtgttat tgattcccaa ttgtctgaac atcaagcaga
    19201 aggtttctta gaaggttatg ttttaactgg tcgtcacggc ttctttgctt catacgaatc
    19261 attccttcgc gttgtagatt caatggtaac acaacatttc aaatggttac gtaaatcaaa
    19321 aacacacaca acatggcgta aaaattatcc agcgcttaac ctaattgcag cttcaactgt
    19381 tttccaacaa gatcataatg gttatactca ccaagatcca ggtattttaa ctcacttagc
    19441 tgaaaaaaca cctgaataca ttcgtgaata tttaccagca gatactaact cacttctagc
    19501 tgttatggat aaagcgttta aagctgaaga taaaattaat ttaattgtga catctaagca
    19561 ccctcgtcca caattttact ctattgctga agcagaagag ttagtggcag aaggatataa
    19621 ggtgattgac tgggcttcaa atgtgtcgct taatcaagag ccagatgttg tctttgctgc
    19681 ggcgggaaca gaacctaact tagaagcttt ggcagcaatt agcattcttc ataaggcttt
    19741 cccagaactt aagattagat ttgttaatgt attagacatc ctaaaacttc gtcatccttc
    19801 acaagatgct cgcggtttat cagatgaaga gtttaacaaa gtctttacca cagataaacc
    19861 tgttattttt gctttccatg gttatgaaga tatgattcga gatattttct ttagtcgtca
    19921 caatcataat ttgcatacgc atggttaccg tgaaaatggt gacatcacaa cgccatttga
    19981 tatgcgcgtg atgtcagaat tggaccgatt ccatctggca caggatgcag ctcttgcttc
    20041 tttaggacat gaagcgcaag ccttcagtga tgaaatgaat caaatggttg cttatcacaa
    20101 agactatatt cgtgaacacg gagacgatat cccagaagta caaaattgga aatgggaaaa
    20161 tatcaagtag tcactttttc aggcctatac ttacaaacaa aaacactttt agattttttc
    20221 taagggtgtt ttgttatttt atagaagaat gaccgctttt tcaaaagcgt tgagtggtca
    20281 cttttaaagt tgtaatatta atagtaataa gtctttaaat aattcaataa tgtcataaca
    20341 ataatggtgt gggctaatta aaatatctct aagcaaaggt gttgacataa agagaccata
    20401 catgatatta tagttatgtt ctgtttcggc agtttatttt tttgagtaaa aaagtgacta
    20461 cccttttacg ataacttaac gtcactttat agataagaca cataacctta aaaataagat
    20521 acgaaatttt tagtaggaga taagaatgac aaatatttta gaagtgaaga atttaaccaa
    20581 aatttttggg aaaaagcaaa aagctgctct tgaaatggtt aagcaaggaa aaagtaagac
    20641 agaaattctt gaaaaaacag gagcgactgt aggtgtctat gatgcgagtt ttgaaataaa
    20701 agaaggcgaa attttcgtta tcatgggact ttctggttca ggaaaatcaa ctttagttcg
    20761 gatgttaaac cgtttgattg acccatcatc aggaaatatc tatttagatg gtaaagatat
    20821 tgcaaagatg aatgtagaag atttacgtaa cattcgtcgt cacgatatca acatggtttt
    20881 ccaaaatttt ggtcttttcc cacatcgtac aattttagaa aatacagagt ttgggttaga
    20941 gatgcgtggt gtttctaagg aagaacgtac aacgttagct gaaaaagctt tggacaatgc
    21001 aggtctatta ccgtttaaag atcaataccc cagtcaatta tcaggaggaa tgcaacagcg
    21061 tgttggttta gcacgtgccc ttgctaacag ccctaaaatc cttctgatgg atgaggcctt
    21121 ctcagcttta gacccattaa ttcgtcgtga aatgcaagat gagcttcttg atcttcaaga
    21181 cacgaataaa caaactatta tttttatcag ccatgatttg aatgaagcgt tacgtattgg
    21241 tgatcgtatt gctcttatga aagatggaga aattatgcag attggtacag gagaagagat
    21301 tttgactaat ccggctaatg attttgttcg tgagtttgtt gaagacgttg atcgctcaaa
    21361 agtgttaact gctcaaaata ttatgataaa acctttaaca actgttttag aaattgatgg
    21421 cccacaagtt gctttaacaa gaatgcatcg tgaggaagtc tcaatgttaa tggcaacgaa
    21481 tcgtcgtcgc caacttctgg gatccctaac cgcagatgct gcaattgaag ctcgtaagaa
    21541 aggtttgccg ctttctgaag tgattgataa agatgttgta acggtctcaa aagatacagt
    21601 tattacagac atcatgcctt taatttatga ttcatctgct ccgatagccg ttactgatga
    21661 taatgataga ctcctagggg ttatcattag aggacgtgtg atcgaagcac ttgctaacgt
    21721 tcaagatgaa acagtagtag aatcgccaaa agaaacggta gaagcttaat agagaggaca
    21781 aatcattttg gaaaatttat tacaacataa gttaccggtc gcaccatttg tagagtcagc
    21841 gaccaattgg attacgaaaa ctttttctgg tttatttgac tttatccaga caatcggtaa
    21901 tgcattgatg gattggatga caaaaacatt actattcatt aatcctttac tttttattgt
    21961 actaattaca gctgctgtat ttttcttagc taagaaaaaa tggcaattac cgacatttac
    22021 tttcattggt ttgctattta tctataacca agggctgtgg gagcagttga ttaacacttt
    22081 taacttggtt ttagtagcaa gtttaatttc tattattatt ggtgtaccac taggtatttg
    22141 gatggcaaaa agtgataaag ttaaacaagt tgttaatcca attcttgact ttatgcaaac
    22201 tatgccagca ttcgtttacc ttattcctgc agtagccttc tttggtatcg gtatggtacc
    22261 aggtgtcttt gcctctgttg tatttgcttt gcctccaaca gtgcgcttta ctaacttggc
    22321 tattcgtgaa atcccacttg agttaataga agcatctgat tcatttggtt caacagctaa
    22381 gcaaaaatta ttcaaagttg aactaccact tgctaagaac actattatgg caggaatcaa
    22441 tcaaactatg atgttggctc tttctatggt ggttacagga tctatgatcg gtgcacctgg
    22501 acttggtcgt gaagtgttat cggcgcttca acatgctgac attggtacag ggtttgttag
    22561 tggtctatcg ctagttattt tagcaatcgt gttagaccgt gttagtcaat tctttaattc
    22621 taaaccgggt gagaagcaag ctaaaacatc aaaagttaaa aaatgggtag gtttaggggc
    22681 cctggctctc ttcattctag cagctcttgg tcgcattgtt gtcaatatga cttcaggaaa
    22741 tgaagccaaa ggtcaaaaag tgaaaatagc ttatgtccaa tgggattcag aagttgcttc
    22801 aaccaacgtt attgctgaag ttttaaaatc taaaggatat gatgttgaat tgacaccact
    22861 tgataatgca gtcatgtggc aaacagttgc caatgggaat gctgatttta caacaagcgc
    22921 ttggttacca aagacccacg ggcaatattt taataaatac aaaaatagtt tagatgactt
    22981 aggtcctcac gttgagaacg tcaaaatagg tttagttgtt cctaaataca tgaacgtaaa
    23041 tagtattgaa gaattatcaa atcaagcaga taaacaaatt acaggtatcg aaccaggtgc
    23101 aggtattatg aagtctgcta aacaatccct taaagattat ccaaatcttt caagttggaa
    23161 gttgttatca gcatcaacag gagctatgac aacaacctta gggaaggcta tcaaaaacaa
    23221 agatcaagtg gtcattacag gttggtcacc tcactggatg tttgctaagt atgatttaaa
    23281 ataccttaaa gatcctaaga aatcatttgg tggtgaagaa catatcaata caatagcacg
    23341 caagaatttg aaaaaagata tgccaaaagt atataaaatt atcgataagt tcaagtggac
    23401 taaagaagat atggaatcta tcatgttaga tatggataaa ggtatggagc ctgctaaagc
    23461 agctcaaaaa tggattaaaa accacaaaaa agaagtttca gagtggacta aatagtatag
    23521 aaaagaaaca cgtctcttta gagcgtgttt tttaatgtga cttaagtcgt ttcagttatt
//
LOCUS       DATRSD010000025        23501 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRSD010000025 DATRSD010000000
VERSION     DATRSD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23501
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.025"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            172..1050
                     /locus_tag="VBD85_000794"
     CDS             172..1050
                     /locus_tag="VBD85_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7133359.1"
                     /translation="MRESIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            1157..1747
                     /locus_tag="VBD85_000795"
     CDS             1157..1747
                     /locus_tag="VBD85_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7133360.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYYNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1781..3052
                     /locus_tag="VBD85_000796"
     CDS             1781..3052
                     /locus_tag="VBD85_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO7133361.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLNGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            3065..3496
                     /locus_tag="VBD85_000797"
     CDS             3065..3496
                     /locus_tag="VBD85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133362.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3588..4472
                     /gene="truB"
                     /locus_tag="VBD85_000798"
     CDS             3588..4472
                     /gene="truB"
                     /locus_tag="VBD85_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO7133363.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4485..5417
                     /locus_tag="VBD85_000799"
     CDS             4485..5417
                     /locus_tag="VBD85_000799"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO7133364.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5460..5873
                     /locus_tag="VBD85_000800"
     CDS             5460..5873
                     /locus_tag="VBD85_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO7133365.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5866..6147
                     /locus_tag="VBD85_000801"
     CDS             5866..6147
                     /locus_tag="VBD85_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO7133366.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSIKVDDLLD
                     SYKEFKKVVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            6137..6901
                     /locus_tag="VBD85_000802"
     CDS             6137..6901
                     /locus_tag="VBD85_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO7133367.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6969..8279
                     /locus_tag="VBD85_000803"
     CDS             6969..8279
                     /locus_tag="VBD85_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO7133368.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8458..9324
                     /locus_tag="VBD85_000804"
     CDS             8458..9324
                     /locus_tag="VBD85_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO7133369.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9370..10287
                     /gene="pstC"
                     /locus_tag="VBD85_000805"
     CDS             9370..10287
                     /gene="pstC"
                     /locus_tag="VBD85_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7133370.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGAFPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10277..11164
                     /gene="pstA"
                     /locus_tag="VBD85_000806"
     CDS             10277..11164
                     /gene="pstA"
                     /locus_tag="VBD85_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7133371.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11176..11979
                     /gene="pstB"
                     /locus_tag="VBD85_000807"
     CDS             11176..11979
                     /gene="pstB"
                     /locus_tag="VBD85_000807"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7133372.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11991..12749
                     /gene="pstB"
                     /locus_tag="VBD85_000808"
     CDS             11991..12749
                     /gene="pstB"
                     /locus_tag="VBD85_000808"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7133373.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12783..13436
                     /gene="phoU"
                     /locus_tag="VBD85_000809"
     CDS             12783..13436
                     /gene="phoU"
                     /locus_tag="VBD85_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7133374.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13582..16131
                     /locus_tag="VBD85_000810"
     CDS             13582..16131
                     /locus_tag="VBD85_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO7133375.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16293..16973
                     /locus_tag="VBD85_000811"
     CDS             16293..16973
                     /locus_tag="VBD85_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133376.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16957..18270
                     /locus_tag="VBD85_000812"
     CDS             16957..18270
                     /locus_tag="VBD85_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7133377.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18359..18691
                     /locus_tag="VBD85_000813"
     CDS             18359..18691
                     /locus_tag="VBD85_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO7133378.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18709..20274
                     /gene="ffh"
                     /locus_tag="VBD85_000814"
     CDS             18709..20274
                     /gene="ffh"
                     /locus_tag="VBD85_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO7133379.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20341..21027
                     /locus_tag="VBD85_000815"
     CDS             20341..21027
                     /locus_tag="VBD85_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO7133380.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKPSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            21017..21802
                     /locus_tag="VBD85_000816"
     CDS             21017..21802
                     /locus_tag="VBD85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7133381.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     GLWRHLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAF
                     IIDQLAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNK
                     FQAKELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGL
                     VFTAKSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(21835..23496)
                     /locus_tag="VBD85_000817"
     CDS             complement(21835..23496)
                     /locus_tag="VBD85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7133382.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYQANKNNKDVVDASDARTTYIIYNQTGSVKALTNQKIRQALNLATDRKGVVK
                     AAVDTGSTPAESLVPKKLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSL
                     KLTITADSDSPAAKNAVDYVKSTWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFS
                     WGGDYPEGSTFYGLFTTNSAYNYGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEK
                     ALFDQSYYNPVYYLGKKGLQNSKLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         7952 a         3949 c         4317 g         7283 t
ORIGIN      
        1 ggttttatag ttaaccatca attaaaaaaa ctcaatatgt tagatgcctt gaaatcagta
       61 gattagttta taataaagat tatcttttct aatcttttcg ataacgattg acagcaagta
      121 atcttaattg tataatataa cagtataaag ttagataaaa gtgagatatt tatgagagag
      181 tctatttttg aggaaactgc aaaaggtaaa gtatcaaggc atgtcctttt aactatatta
      241 ttggctttga tattactatt tacagcagat ttccttacct atattattat tgaattgatt
      301 atctcgcttg atttttggaa actagagtgg gatagcgata tcgtccagtt aatcatttta
      361 ctagcaactt caattagtac ggtaatgtta tttatgtggg tgaaatttaa agagaagcgt
      421 ccaattagtt ctctagggct atttaaagaa ggagccctaa cagaattatc tagaggatgg
      481 ttagtcggga gcgttctctt tggagcctgc ttaatattaa caataatttc aggctctgga
      541 gttatcgaaa ctgttcgttt tacagctgat aaattgattt ggtttcttat ttttgctgtt
      601 ggatggcagg tacaaagcgc atctgttgaa atattgtgtc gtggatggtt acttccggtg
      661 agtgccacaa agtatagtaa gatagtttct gtcagtatca gttcaatctt tttcggtctt
      721 ctgcatagcg ctaataatca tgtaagcctt atctcgattt tcaatctgtg tctctttgga
      781 ctatttctat cgctctatgt tattttaaaa ggtaacatct ggggggcgtg tggtattcat
      841 ggcgcctgga actgtgtaca agggagtgtc tttggaatcg aagttagtgg agagccaatg
      901 ttgtctaact ccctagtcca tgttaaaacc tatggtgctg actggattag cggtggaaaa
      961 tttggtgtgg aaggaagcat gattaccagt attgttctta tcgtagcatg ttattggctt
     1021 tatcaaaaat cgaattttcc ttacgactaa tagatttaat ctaaataagc ttctcttact
     1081 gaaagcttat tttttatttt agtattaaaa aactatgttt tgtgatataa tattttaaag
     1141 acgaaaggag tctattgtgc cacaaaatcc aacaagtaac ccagagaaaa aaacttattt
     1201 attatctgaa ttggtagact gctttaaagg taaagctgtg ccttcaaaag cagaagcagg
     1261 tgatatccgt attattaatt tgagtgatat gtctccttta ggaattgact actataactt
     1321 aagaacattc caagatgaac aacgatcgct tttaaaatat ctacttcagg aaggcgatgt
     1381 tttaatcgct tcaaaaggaa ctgtcaagaa agttgctatt tttgaagagc aagattaccc
     1441 tgtcgttgcc tctgctaata ttacaatact aaggcctaca caacacatta gagggtacta
     1501 cttaaagctt ttttttgatt cagaagaagg tcaacaggca cttgagaatg ctaacaaagg
     1561 aaaagctgtg atgaatatta gcacaaagga actgttaaat attgcaatac caagtatccc
     1621 gctttttaga caggattatc ttatccaacg ttataaacag gggctcaacg attacaaacg
     1681 taagatagca cgtgcagagc aggagtggga acgtatccaa aatgacatca gacaacaact
     1741 tagctagaac caaatacaaa acatcatagg aagagttatc atgaacgaaa tcaaatgccc
     1801 tcattgtgga acagcttttg ccatcaacga gtctgaatac catcaattac tagaacaaat
     1861 tcgcggagat gcttttgaca aagaagtaag tgaacggttg gaaaaagaac gtctaatatt
     1921 aggggagcaa gcaaaaaatc aattacagga agttgttgta gaaaaagaca aggagatagc
     1981 taaacttcag tacaaagtca aacaatttct tatagaaaaa gacaatcttc tcaaagacaa
     2041 tgagtaccaa ctcgctgagc aattaaatca aaaagacatg atgcttcgcg accttgaaaa
     2101 ccaaatcgat agactacgtt tagagcatga aaatagcttg caagaggcgc taacaaaagt
     2161 cgaacgagaa agagatgcaa tacaaaatca gttgcacatt caagaaaaag aaaaagattt
     2221 agctttagct tcagtaaaaa gtgattatga agtacaacta aaggcagcca atgaacaagt
     2281 agaattctat aaaaacttca aagctcaaca gtctactaaa gcagtaggag aaagtttaga
     2341 acattatgct gaaacagaat ttaataaagt gcgacatttg gcctttccta atgcttattt
     2401 tgagaaggac aatacattat caagtcgtgg ctcaaagggg gactttatct atcgagaaaa
     2461 ggatgaaaat gaccttgagt ttttaagtat catgtttgaa atgaaaaatg agtctgatga
     2521 tactatcaag aagcataaaa atgaagattt tttcaaagaa ttagataaag atcgtcgtga
     2581 aaaatcttgc gaatacgcag ttttagtaac tatgcttgaa gcagacaatg actattataa
     2641 tactggaatt gttgatgtta gtcacaaata ccctaaaatg tacgttatac gtccacaatt
     2701 ttttatccaa ttaaatggta ttctaagaaa tgcagcactc aataccttaa aatataaaca
     2761 agagcttgct ttgatgaaag aacaaaatat tgacatcaca cattttgaag aagatttaga
     2821 tattttcaaa aatgcatttg ctaaaaatta taattctgca agcaaaaatt tccagaaagc
     2881 aatcgatgaa atagataaat ctattaaacg tatggaagct gttaaggctg ctttaacaac
     2941 gtctgaaaat caactacgtc ttgcaaataa taaattagac gatgtttctg tcaagaaatt
     3001 aacaagaaaa aatccaacaa tgaaagcaaa attcgatgct ctaaaagact aagaggttat
     3061 gattatgaag attagaactg ctacactaga cgatagtgaa aaattagtcc ctttgtatca
     3121 agaattagga tacgcaatat cattatcaga gattcaatct attttaaagg ttattcttac
     3181 tcattcagat tatggctttt tgatagctga ggataatggc aaactcctcg ccttcgttgg
     3241 atatcataaa ctttactttt ttgaaaaaag tgggacttac tatcgtattt tagcactagt
     3301 agttaatgaa aagcaccgtc gtaaaggcat cgctagtcaa cttattaatc acgttaaaca
     3361 gctagctaaa acagatggaa gtgaagtact ggctttaaat agcagtttaa aagaatatcg
     3421 tcaggaagct tatcattttt atgaaaacct agggttcaag aaggttagta caggcttttc
     3481 atactattta gattaggtat aaatccttat agcactatac caaaagagcg taagttttta
     3541 cgctcctttc tttgtgttac cgcttattta ctgatataat ataaaacatg attacaggaa
     3601 taattaattt aaagaaagaa gctggaatga catcacacga tgctgtattc aagcttcgaa
     3661 agatattaca tacaaaaaaa ataggacacg gcggaactct tgatccagat gttgttgggg
     3721 ttttaccaat tgcggttgga aaagccactc gtgttattga atatatgaca gaaagtggca
     3781 aaatttatga aggagaaatc actttaggat atgccacaag tacagaagat tctagtggag
     3841 aggtcatttc aagaactcca ctaactcaat ccgatcttag cgaggatgtt gtggatcatg
     3901 cgatgaaaag ttttaccggt ccaattacac aagttcctcc tatgtattca gcagttaaag
     3961 taaatggtaa aaaactctat gaatatgctc gttcaggaga agaagtagag cgacctaaac
     4021 gacaaataac aatttctgaa tttagacgca caagcccatt gtacttcgaa aaaggcatct
     4081 gccgttttag tttctatgtc tcatgcagta aaggtaccta tgttaggacg ttagctgttg
     4141 atctaggaat aaaactagga tatgctagcc atatgtcttt cctaaaaaga acctcatctg
     4201 caggattatc tattacacaa tcattaacgc ttgaagaaat taatgaaaaa tataagcaag
     4261 aagatttttc atttttactt ccaattgaat atggtgtttt agatctccca aaagttaacc
     4321 taactgagga agataaagtt gaaatctcat acggacgtcg tatcttatta gaaaatgaag
     4381 cagatacctt agcagctttt tatgaaaata gagttatcgc tatactagaa aaacgtggca
     4441 atgaatttaa accacacaaa gtattgttat aaagaaagtc aatcatgaaa atccattaca
     4501 ttaatgatta taaagatatt caagctaaag aagattgcgt cttagttttg ggctattttg
     4561 atggcttaca tctgggacat aaagccctct ttgataaagc taaaaaaatc gcgaccgaaa
     4621 aaaatttgaa aattgtcgtc ttaacgttta atgagacacc tcgtctaacc ttcgctcgct
     4681 tccaacctga attattgcta catcttacct ctccagaaaa acgttctgaa aaatttcaag
     4741 agtatggcgt cgatgagtta tatttaatga attttaccag tcatttttca aaagtatctt
     4801 ctgacctttt tattaagaaa tatatttatg gtctcagagc aaaagctgct gttgtcggtt
     4861 ttgattataa gttcggtcat aatcgtacca gtggtgatta tctagcgcgt aatttcaagg
     4921 gaccagtcta tattattgat gaaatttctg aaggtggtga aaaaatctct tctacacgta
     4981 ttcgtcaatt gattacagag ggtaatgttg agaaagctaa ccaacttttg ggttatgagt
     5041 tttcaacctg tggcatggta gtacatggag atgctagagg acgaactata gggttcccaa
     5101 ctgctaatct agctcctatt aatcgcactt acttacctgc agacggtgtt tatatttcca
     5161 atgttctgat taatggaaaa tattatagag caatgacgag cattggaaaa aatatcactt
     5221 ttggtggtac agaacttcgt ttagaagcta atatctttga ttttgatggc gacatttatg
     5281 gagaaactat tgaaattttc tggttaaaaa gaatcagaga aatggtaaag tttaatggta
     5341 ttgacgatct tgtaaaacaa cttaaaaaag ataaagaaat tgctttaaat tggaaaaaag
     5401 atagtcaaac tctttaattt tttgtataat agtctctagt aaatacaaga ggcggttata
     5461 tgattacctt atttttatca cccagctgta ctagttgtcg aaaagcgcgt gcttggctaa
     5521 gtaagcatga ggttgctttt gaagagcata acattattac aagtccactc aataaagaag
     5581 aactattaca aattctatcg tttacagaaa atggtacaga agacattatt tccacacgtt
     5641 caaaagtctt tcaaaaactt gctattgatg tagacgaatt atcaacgtct tcattaatgg
     5701 aattaatttc agaaaatcca agtcttttac gacgcccaat tattttagac aaaaaacgta
     5761 tgcagattgg ttttaatgag gatgaaattc gtgccttctt accgcgtgac tatcgtaaac
     5821 aagaactgaa acaggccact attcgtgctg aaattgaggg aaaacatgat tagtagtaat
     5881 tatagttatc ctcttgatcc ttcatggaat acggaagata ttactaaggt acttcgcttt
     5941 ttaaatcaag ttgaacacgc ctatgaaaat agtattaaag ttgatgattt gctagatagc
     6001 tataaagaat ttaaaaaagt tgttaaatcc aaggcgcagg aaaaacaaat tgaccgtgaa
     6061 ttccagagaa ctagcggtta ttccacttat caagctgtta aagcagcaca acaacaagcg
     6121 aaaggattta tttcacttgg acgctaaatt tgatttcgct aaacaattag tgtataaagc
     6181 tggtcaattc attaaatctg agatgcaaaa tacttttgat gttgaggaaa agtctcgatt
     6241 tgatgattta gtgacaagtc ttgataaaaa aacacaaaaa ttattaattc aagaaattat
     6301 tcaacattac ccagatgata atatacttgc cgaagaagat gatgtgcgct cacccattgc
     6361 acaaggtaat gtttgggttt tagaccctat tgatggaact gttaatttta ttgttcaaaa
     6421 agataacttt gctgttatgt tagcttatta cgaagaaggc gttggacaat ttggtatcat
     6481 ctacgacgtt atggctgata ttctttacag cgggggagga cattttgatg tctatgctaa
     6541 cgataaaaaa attgttcctt ttcaagagtg tcctttagaa cgttgcttac ttggagtaaa
     6601 ttctgctatg tatgcagaaa atgattgcgg tattgcccat ttagcttctg aaaccttagg
     6661 tgttcgtatc tatggtggtg ctggaattag catggcaaaa gtcatgcagg gcaaattatt
     6721 agcttatttt tcgtatattc aaccttggga ttatgctgca gcaaaaatca tgggggagac
     6781 attaggattt accttattaa cgcttgacgg ggaagaaccc aactattcaa caagacaaaa
     6841 agtaatgttc ctaccaaagt cgaaattaaa tcttatccaa tcctacctta ccaaaaaata
     6901 aacttaaaac cttctaatta aatagaaggt ttttttgcac gcccgtgatt atgtagtata
     6961 atagaaacat gaaattaccg aatgaattta ttgaaaaata tcagactata cttaaagatg
     7021 aagcagaagc tttttttgac agttttgaac aaaaacccat atcggcatat cgcaccaatc
     7081 ctctcaaaga aaagcaactt gattttccaa atgccatccc aagtacccct tgggggcatt
     7141 atgggaaaat atcaggaaaa tcaatagaac acacaactgg ccttgtttat tctcaagaac
     7201 ctgctgctca gatagtggct cagattgctg aaccacaaga aggaatgaaa gtgctagatt
     7261 tagcagctgc acctggtgga aaaacaacac accttctatc atatttaaac aatactggtc
     7321 tattagttag taatgaaatt tcaaataaac gtagtaaaat tttagtcgaa aatgtcgaac
     7381 gttttggtgc tagaaatgtt atcgtgacta atgaaagctc ccaacgacta gccaaatgtt
     7441 ttaattcttt ttttgatctt attgtttttg acggtccgtg ttctggtgag ggaatgttcc
     7501 gcaaagatcc tcaagcaata caatattggc acaaagatta ccccactgaa tgtgcacagt
     7561 tacaaagaga tattttaaaa gaagcaatca aaatgctagc tcatggtggt atacttgtat
     7621 actctacttg tacatggtca ccagaagaga acgaagaagt ggttaattgg ctccttcaag
     7681 agtatgatta cttagaactt gttgatattc ctaaactaaa tgggatggtt gaagggatta
     7741 atgtaccaca agttgcaagg atgtaccctc atcattttca aggtgaagga caattcgttg
     7801 caaaactgag agatactaga tctaaggaag cacagaaaat taagccaaaa gcacagaaaa
     7861 taaataaaat gcagttacaa ttgtggcaac aatttgcaca agaccattta aagatagact
     7921 tgaacggtgt tttagatgtt ttcggcgacc aactctatct tctacctaat ggtctccccg
     7981 atttgtctaa attaaaaata gcacgcaatg gtctccatct aggcactttc aagaagaatc
     8041 gttttgagcc atcgtttgct ttaggaatgg cacttagcga acatgaccta gtacagtcta
     8101 ttgaaattga tatagaacag tttgaggtgt acgtatctgg aaatgtagtc aaactagata
     8161 agactgttcc aaacggttgg tatcaaattc ttgtaaaagg caatggatta ggttttgcaa
     8221 aagtgacaaa taatactctt aaaaattatt accctaaagg actacgattt cagacataac
     8281 tgttattgaa tacttttaat aaaaaaggaa aaagaaatac ttttcaaacc ctagaaactt
     8341 atggtactat gggacagtga tgtgtgtttt tactatttgt aaatcattaa acactcaatg
     8401 attttagtaa tatatatcaa ctactaaata tcaaaattta ttcaaaagaa taggaaaatg
     8461 aaaatgaaaa aaaacatttt tttgttaacc ttgctagcca ccgtcttatt gacgctgtct
     8521 ggttgtgcta attggattga taaaggtcaa tcaatcacca gcgttggttc aaccgcctta
     8581 cagccacttg tagaagctgc agcagatgag tttggtaaga caaacctcgg taagactatc
     8641 aatgtccaag gaggaggctc aggaacaggc ttatcacaag tacagtctgg agctgttcaa
     8701 attggtaata gcgacttatt tgcagaagaa aaagatggta ttgatgctaa gaaattgaaa
     8761 gaccatcaag tcgcagtagc tggattagct gttattgtta ataaaaaagt taatgttaaa
     8821 aatctaacca cccaccaact tcgtgatatt tttgcaggaa aaatcaagaa ttggaaagaa
     8881 gtgggaggac aagatttaga tatttccatc atcaatcgag cagcaagttc tggatcccgt
     8941 gcaaccttcg ataatactat tatgggaaat gttgctccta ttcaaagcca agaacaagat
     9001 tcaaatggta tggtgaaatc aattgtttct caaacacctg gtgcgatttc atacctcgct
     9061 tttgcatacg ttgataaatc tgtcggaaca ttaaaactaa atggttttgc gccaacagct
     9121 aaaaatgtta ctaccgacaa ctggaaactt tggtcatatg aacatatgta tacaaaaggt
     9181 aatgaaacag gtctaacaaa agaattctta gactacatga aaagtgataa agttcaaagt
     9241 agtatcgtac aacatatggg atatatctct attaacgaca tgaaagttgt taaagatgct
     9301 gaaggtaaag taaccccaaa ataaaactta atcacatcta gataaagcat cttgatccta
     9361 ggaggaatta tgaaaaatca agaattagct aaaaaattaa catctccttc aaaaaactct
     9421 cgattagaaa aatttggtaa aggaataact tttctttcgc ttgccctaat cgtcttcatt
     9481 gttgcgatga ttttgatttt tgttgctcaa aaaggactat ctacattttt tgttgatggc
     9541 gttaaattaa cagacttctt atttaataca aaatgggaac caagtgctaa gtcatttggt
     9601 gcttttccaa tgatagctgg atcatttatt gttactattc tatcagctat cattgctaca
     9661 ccatttgcta tcggtgctgc cgtatttatg actgaaatct cacctaaata tggttctaaa
     9721 attttacaac ctgcagttga attactagtt gggattcctt ctgttgttta cggttttatt
     9781 ggtttacaga ttattgttcc cttcgttcgc tctatttttg ggggaacagg ttttggcatt
     9841 ttatctgggg tctgtgtcct cttcgtcatg attctaccaa ctgttacttt catgacagta
     9901 gatagcctta gagctgtgcc acgtcactat aaagaagcaa gtttagccat gggggcaact
     9961 cgttggcaaa caatttggcg tgttatatta aacgctgcac gtccaggaat tttcactgca
    10021 attgtttttg gtatggcacg tgcatttggt gaagctctag caattcagat ggttgtcgga
    10081 aactctgcaa ttttaccaac gtcattgaca acaccagcag caacattaac gtctgttcta
    10141 acaatgggta ttggtaatac tgtaatggga actgtacaaa acaatgtcct ctggtcgcta
    10201 gccttagtac ttttaattat gagtttagca tttaatacag ttattaagtt aattacaagg
    10261 gaaggaaaga aaaactatga acgctaaaaa ggctgacaaa ttagcaacta caatactcta
    10321 tagtattgca gctatcatcg ttacaatcct cgcctctctt ttaattttta ttcttgttcg
    10381 cggattgcca cacgtatctt ggtcattttt aactgggaaa tcatcatcat atgaagcagg
    10441 aggcggtatt ggaatacaat tatacaattc ctttttccta cttattgtta ctctgattat
    10501 ttctattcct ctatctttag gagctggaat atacctctca gaatatgcta aaaaaggacg
    10561 tttaacaaac tttgttagaa catgtatcga aattttgtca tctcttcctt ctgttgtagt
    10621 tggtctgttt ggttacctaa tcttcgttgt tcaatttcaa tatggcttct caattatctc
    10681 aggtgcctta gcacttactg tttttaactt accccaaatg acacgtagtg ttgaagatag
    10741 ccttcaaaat gtacaccaca cacagcgtga agcaggattg gccctaggta tttctcgttg
    10801 ggaaacggtc atttacgtcg ttgtccctga agctctgcca agtattgtaa ctggtgttgt
    10861 gctagcttca ggacgtattt tcggtgaggc agcagcactt atttatacag ctggtcaatc
    10921 tgctccagca cttgattggt caaattggaa cgtccttagt gttacaagtc caatctcaat
    10981 tttccgtcaa gcagaaactt tagctgttca tatttggaaa gttaatagcg aagggacaat
    11041 ccctgatgca acacaagtat ctgccggtag cgccgcagtc ttattggttg ttattcttat
    11101 tttcaacctt tcagctcgct cgatcggtaa gaaacttcat tcgaaattaa cctcgtctaa
    11161 ttaaaaggag tagtaatggc agaatataat tgggacgaac gccatatcat taccttccct
    11221 gaagaaaatt cagctctcac cacaaaagat ttacacgttt actacggtga gaaagaagct
    11281 atcaagggaa ttgatatgca atttgaaaaa aataaaatta cagccttaat cggcccatct
    11341 ggatgtggaa aatcaacata tcttcgtagc ttaaatcgta tgaatgatac gattgatatt
    11401 gcacgcgtga ctggacaaat catgtatgaa ggaattgatg ttaacgctca agatatcaat
    11461 gtttacgaga tgcgaaaaca tatcggaatg gttttccaac gtccaaaccc gtttgcaaaa
    11521 tcaatttata aaaacattac ctttgcatat gagcgtgctg gtgtaaaaga taaaaaattc
    11581 ttagatgaag ttgtcgaaac ctcactaaaa caagctgctc tatgggatca agtaaaagat
    11641 gaccttcata aatcagcatt cactttatct ggcggtcaac agcagcgatt atgtattgca
    11701 cgtgctatcg cagttaaacc agaaattctc ttaatggatg aacctgcttc agcactcgat
    11761 ccgattgcaa ctatgcaact agaagagaca atgtttgaac tcaagaaaaa ttacactata
    11821 attatcgtaa cacataatat gcaacaagca gcacgtgcta gtgattatac tgcatttttc
    11881 tatcttggag acttaatcga atacgataaa acgaataaca ttttccaaaa tgctaaatgt
    11941 caatctacta gcgattatgt ttctggtcga tttggatagt gaggtaaaag atgacacaac
    12001 ctattttaca agtcagtgat ttgtctgttt actataataa gaaaaaagct ttaaaagaag
    12061 tctcaatgga cttctatcct aatgaaatta cagccttaat tggtccgtct ggttctggaa
    12121 agtcaacact cttacgagct attaaccgta tgggagactt aaatcctgaa gtcactctaa
    12181 caggagcagt gatgtataac ggacataatg tttatagccc gcgcactgat accgtagaac
    12241 ttcgtaaaga aattgggatg gtttttcagc agcctaatcc atttcctatg tcagtatttg
    12301 aaaatgttgt ttatggtctt agactcaaag gaataaaaga caaagctacg ttagatgaag
    12361 ccgtagagac ttcattgaag ggcgcgtcta tctgggacga ggtcaaagat agattacacg
    12421 actctgctct tggtttatct ggtggtcaac aacaacgtgt ttgtattgcc agaacccttg
    12481 ctaccaagcc taaaattatc cttctagatg agccaacctc tgcacttgac ccaatttctg
    12541 ctggtaaaat tgaagagact ttacatggtc taaaagatca atataccatg ttacttgtta
    12601 cacgttctat gcagcaagcc tcacgtattt ctgatcgtac aggtttcttc ttagacggta
    12661 acttaattga atatggcaat acaaaagaaa tgtttatgaa tcctaaacat aaagaaactg
    12721 aagattacat tacaggtaaa ttcggttaat accatataat aagatcagaa agagaataca
    12781 atatgttaag atcaaaattt gatgaagaat tagataaact gcataaccaa ttctatgcaa
    12841 tgggaattga agctatcgga caaatcaaaa aaacagttcg tgcttttgtc agtcatgatc
    12901 gagagttagc taaagaagtg atagaagatg atgtaacgct taacaacttt gaaacgaagt
    12961 tagagaaaaa atcattggaa attattgcac tccagcagcc tgtatcacaa gacttacgca
    13021 ccgttattac cgttttaaaa gctactagtg atgttgagcg tatgggagac catgcagcag
    13081 cagttgcaaa agcaaccatc cgtatgaagg gcgaggaaag aatcccagca gtagaattag
    13141 aaatcaataa tatgggaaaa gcagttaaaa acatgcttga agaagcctta acagcttata
    13201 tcaatggtga tgatgaaaaa gcttacgaag tagcagcaat ggatgaaata gttgatgatt
    13261 acttccgtga tatccaaaaa atggtagttg aaacaattca aaagcatcct gacgtagcat
    13321 ttgccgccaa agaatacttc caagtattga tgcacttgga gcgaattgga gattatggca
    13381 aaaatatctg tgaatggatt gtttatttaa aaactggaaa aattatcgag ttatgatata
    13441 attattgctt gtagagtggt aattgtaagt atagttcgtg cgatagaaaa gctgtaacta
    13501 tcttgttaca gcttttctat cttttctagc ccatacagga ctagtaatgt ttcaaatgtt
    13561 tctgaattag ttaggagtta tatgaaaact gtagaacatt ttgttaccaa atttgtccct
    13621 gaaaactata accttttctt ggatattaat cgtcaaacta aaaccttttc aggtaacgtt
    13681 gctgtgtctg gcgaagcctt agataacaat atctcttttc accaaaaagg gttaaccatt
    13741 aaatctgtct tattagataa tcaaccatta gatttccaac tagatgaaga caatgaagct
    13801 atgcatattc aattgcatga aactggtagc atggtattag tctttgaatt cagcggtcac
    13861 attaccgata atatgacagg tatgtatcca tcttactata cagttaatgg catcaaaaaa
    13921 gaggttatct ccacacaatt tgaaagtcat tttgctcgtg aagtattccc aagtattgac
    13981 gaaccagaag caaaagcaac ctttgatttg agcctaaaat tcgatcaaaa agaaggagag
    14041 attgctcttt caaatatgcc ggaaatcaat gctgaacaac gtcaagaaac aggtttatgg
    14101 acatttgaca ccacacctaa aatgtcctca tatttattag cttttgcctt gggggaattg
    14161 catggtaaaa caacacatac caaaaacggc actcttgttg gctcatatgc cacaaaagcc
    14221 catcagttaa atgaattaga tttttcttta gatatcgttg tgcgtgttat tgagttttat
    14281 gaggactact ttggtgtacg ttatcctatt cctcagtcac tccatgttgc tctacctgat
    14341 ttttctgctg gagctatgga aaattgggga ttagtaacat atcgtgaagt ctatctttta
    14401 gtagatgaga actctagtgt ttccagccgt cagcaagtag cattagttgt agcacatgaa
    14461 atagctcacc agtggtttgg taacctcgtt actatgaaat ggtgggatga tttatggtta
    14521 aatgaatcat ttgctaatat gatggaatat gtatcaattg attatattga accaaaactt
    14581 aatatatttg aagactttca aacaggtggt ctaccattag ctcttaagcg tgatgccaca
    14641 gatggcgtcc aatctgttca cgttgaggtc aatcatcctg atgaaattaa cacacttttt
    14701 gatccagcta tcgtttatgc aaaaggtagc cgcctcatgc acatgttacg tcgctggtta
    14761 ggcgatacag attttgccgc aggactaaaa atttattttg aaaaacatca atatcaaaat
    14821 actattggtc gtgacctttg gaatgcactt tctcaaacct caggaaaaga tgttgcagct
    14881 ttcatggatt cttggttaga gcaaccagga taccctgtga tggcagccaa aatagaagaa
    14941 gatgagctca ttttaactca aaaacaattc tttattggtg aacacgaaga taagtctcgc
    15001 ttgtggcaaa ttcctcttaa tagtaattgg gaaggaatcc cagagatatt aaccgaggaa
    15061 acggtagtaa tccccaattt tagtcagtta gctgaaaaga acaaagaaaa tggtgcactg
    15121 cgtttcaata ctgaaaatac tgcacattac attactaact atcaaggtca actcttagag
    15181 catatcattt ctgatcttcc tcttatggat aacatttcta aactacaaat tgtacaagaa
    15241 cgtcatttac ttgctgaaag tggtatgatt tcatatagta gtttaatacc tttagtctct
    15301 cttttgagcc aagagacatc atatcttgtt aactctgcta ttaagagtgt tattgatggt
    15361 ttatccttat tcgtccagga agatagccaa gatgaattcg actttaaaga atttgttaat
    15421 aaattatcag catttaattt taatagactt ggctttgaaa aaagagaagg agaaggagat
    15481 gatagcgaaa tggtccgtca tctatcccta agccttgctc tctactcaga taatgaacat
    15541 gctattgaag aagctcacca tatttttaaa gcccacgaaa ataatatcgc tgctatacca
    15601 gctgcaattc gcctgttagt tcttaccaat gaaatgaagc atttcgaatc caaagaactt
    15661 agccacttgt tattagagac ttatagtact actaccgatg ggaatttcaa acgacaacta
    15721 gcgagtgctt tatctcatac tacagatagc aagacactca agaaattatt gtcagattgg
    15781 aaaaataaag atattgtgaa accacaagac ttagctatga gttggtatgc tactttcctt
    15841 aaaaattctt ttacacaaga aagtgtctgg gagtgggccc aagaaaactg ggaatggatc
    15901 aaagcaactc taggtggtga tatgagtttt gataaatttg tgatttatcc ctcaagtagt
    15961 ttcaaaacag aagaacgcct tgaacaatat aaaaactttt tcgagcctca attaagcgat
    16021 atggcgatta gccgtaatat ttcaatggga attaaagaaa tttctgctcg tgtcctttta
    16081 atcacgaagc aaaaagaaga agttatcaat actatcaaaa aatatatcta ataaactcaa
    16141 atagagattg atggattatt caatctctat ttctttgtct gtgtcatgaa attagttgac
    16201 ataaaacata ctttatttag gatttattaa gcaaagtttg gtattctaat attaatagtt
    16261 cagataaaat atgtacatcg aaaggatata ctatgataaa aattttatta attgaagatg
    16321 acttgagtct ttccaattcc gtttttgatt tccttgatga ctttgccgat gtcatgcaaa
    16381 tttttgatgg agaagagggt ctttacgaag ccgaaagtgg agtctatgat ctgattcttc
    16441 tagatctcat gttacctgaa aaaaacggct tccaagtgtt gaaagagcta cgtgaaaaag
    16501 gtattacaac ccctgtatta ataatgaccg ctaaagaaag cattgatgat aagggacaag
    16561 gtttcgactt aggtgctgat gattatctaa caaaaccttt ttacctagaa gaactaaaaa
    16621 tgcgaattca ggctctttta aagcgttctg gaaaatttaa tgataacagc ctaatttatg
    16681 gtgatattcg cgtagacatg tctactaact caacctttgt taatcaaaca gaagttgagc
    16741 tcttaggtaa agagtttgat cttttagtct atttccttca aaatcaaaat gttatcttac
    16801 cgaaatctca aatctttgat cgtatttggg gatttgatag tgatacaacg atttcagttg
    16861 tagaagtcta tgtctctaag gttcggaaaa aattaaaagg aacacttttt tcagaaaacc
    16921 ttcaaacttt gcgtagtgta ggatatattt taaagcatgt cgaaaccgat taagataagt
    16981 aaattcaaaa aaaatgtctc agatagtcat tttatccatt ttttcacagt ttttagtggt
    17041 atttttctgg ttatgactgt tatcatcctc caagtcatgc gctatggtgt ttattcttct
    17101 gttgatagta gtttaaagta tatcagtacc catcctaaaa attacatcaa tatggttatg
    17161 tcacgtactg ctgcctacct agataactcc aatatcgcaa gtgtcaagtt aaaacccggc
    17221 ggtcagacag tagctaatac tgatattatt ctttttacat cagaagaaga agtcattaac
    17281 tattttgacg cattttcaaa ctatcagttt ttaaaaccta ataaaaaaaa tctaggtggc
    17341 attagtgaat taactttaac caacatcttt ggtcaagatg agacatatca tgctgtcacc
    17401 gtaaaagtta ataaccctgc ttatcctaat gtgacttaca tgacagctat tgttaatatt
    17461 gatcaattag taaatgctaa agaacgctat gaaaaaatta ttatatttgt catgactact
    17521 ttttggatta tttcaattgg agcaagtatc tacttagcta aatgggcaca aaaaccgatt
    17581 atagaaaact acgaaagaca gaaggctttt gtcgaaaatg ctagccacga gctgcgtact
    17641 cccctagctg tcttacaaaa tcgcctagaa acgctattca gaaaaccgaa tgccaccatt
    17701 ttagaaaata gtgaaaatat cgcttcaagt ctagatgaag tacgaaatat gcgtatttta
    17761 accactaatc ttttaaactt agcaagacgt gatgatggaa tcaaaccaga attagctgtt
    17821 attaagccta cactttttga tagtatcttt gagaattatg atctaatcgc ccaagagaat
    17881 ggtaaaaatt ttactggaca taatatgatc caagatagtt tcaaaacaga taagacactg
    17941 ctaaaacaac tcatgactat tttatttgat aatgctataa aatatactga caatgatggt
    18001 agcattgact ttaccatatc tgaaactgat aagtatctct ttttagaaat cgctgataat
    18061 ggaccaggaa tttcagagga ggataaagtc agaatttttg atcgttttta ccgtgttgac
    18121 aaagcaagaa cacgacaaca aggagggttt ggcctcggcc tttctttagc tcaacaaatc
    18181 gttaactcac tgcgaggtaa tattacagta attgataata aacctagagg cagtattttt
    18241 aagataaaac taccaaaagt aaaaggttag gccatgttag tcctaacctt ttaaatttta
    18301 aagtggtctt gcatacttga tatagcaagc tgataggaaa tttggtataa taaaagctat
    18361 ggaaattgaa aaaacaaatc gtatgaatgc cctttttgag ttttatgcgg cattattaac
    18421 agataaacaa atgaattata tcgagttata ctacgccgat gactacagcc ttgctgaaat
    18481 agctgaagaa tccggtgtta gccgacaagc tgtctatgat aatatcaaac gaactgaaaa
    18541 aattcttgag gcgtacgaaa tgaaactaca tatgtattca gattatattg tccgtagtca
    18601 gatttttgat gacattctag aaaaatatac cgatgatgcc tttttacaag aaaaaatttc
    18661 cattctaagt agcattgata atagagatta aatgaggaga aataatttat ggctttcgaa
    18721 agtttaacag agcgcctgca aggagttttt aaaaatattc gtggtaagaa aaagttatct
    18781 gaaaaagatg tgcaagaagt caccaaggag attcgtctag ctcttttaga agctgacgtt
    18841 gctcttccag ttgttaaaac ctttattaaa catgttcgtg aacgtgctgt tggacatgaa
    18901 atcattgata cactagatcc tacgcaacaa attgttaaga ttgtcaatga agagctaaca
    18961 gatttattag gggctgagac ctctgaaatt gaaaaatccc ctaaaattcc aaccatcatt
    19021 atgatggttg gtctacaagg tgctggtaaa acaacttttg ccggtaagtt agcaaacaaa
    19081 ctaatcaaag aagacaatgc tcgtcctatg atgatagcag ctgatattta tcgaccagct
    19141 gctattgatc agttaaaaac ccttggtagc cagattaatg ttcctgtctt tgatatggga
    19201 actaaccatt cagcagttga gattgtcact aaaggtttag aacaggctcg tgaaaatcgt
    19261 aatgactatg ttcttattga tacagccgga cgcctacaaa ttgatgcaac ccttatgcag
    19321 gaattgcatg atgtcaaagc catagctcaa cctaatgaaa tactccttgt cgttgacagc
    19381 atgattggac aggaagctgc aaatgtcgct gaagaattta accgtcagct cagcattagt
    19441 ggtgttgttc tgacaaagat tgacggtgat acacgcggtg gtgctgccct ttcagtacgc
    19501 gaaattactg gaaaaccaat taaatttaca gggacagggg aaaaaatcac tgatattgaa
    19561 actttccatc ccgatcgtat ggcaagccgt atattgggga tgggagacct gctaacactt
    19621 attgagcgcg ctagtcaaga atacgacgaa aaacgttcaa tggaattagc ggagaaaatg
    19681 cgtgaaaaca cctttgactt caacgatttt attgatcaat tagatcaagt tcaaaatatg
    19741 gggccaatgg aagatctcct taaaatgctt ccaggaatgg ctaacaatcc agcaatgaaa
    19801 aacttcaaag tggatgaaaa cgaaattgct cgcaaaagag ccattgtgtc atcaatgact
    19861 ccagaagaga gagaaaatcc agatctcctc aatccaagtc gtagacgtcg tattgctgct
    19921 ggatcaggta atactttcgt tgatgttaat aaattcatta aagattttaa ccaggcaaaa
    19981 caaatgatgc aaggcgtgat gtccggtgat atgaataaaa tgatgaaaaa aatgggaatt
    20041 gatcctaata acctccctaa agatatgcct ggaatggacg gaatggatat gtctaattta
    20101 gaaggtatga tggggcaaaa tggcatgcct gacttatcta gtttaggtgg cgatatggac
    20161 ttcagccaaa tgtttggtgg tggcttaaaa ggaaaagtag gagcctttgc agctaaacaa
    20221 tcaatgaaac gtatggctaa taaaatgaag aaagctaaga agaaacgcaa ataaaaatgt
    20281 ctatttaaat agacattttt atttttgtct gctataatag acaaaaaggt ggtgatattt
    20341 atgtatttag cattaatcgg tgatatcatt aattcaaaac agatacttga acgtgaaact
    20401 ttccaacagt cttttcagca actaatgacc gaactatctg atgtatatgg tgaagagctg
    20461 atttctccat tcactattac agctggtgat gaatttcaag ctttattgaa accatcaaaa
    20521 aaggtatttc aaattattga ccatattcaa ctagctctaa aacctgttaa tgtaaggttc
    20581 ggcctcggta caggaaacat tataacatcc atcaattcaa atgaaagtat cggtgctgat
    20641 ggtcctgcct actggcatgc tcgctcagct attaatcata tacatgataa aaatgattat
    20701 ggaacagttc aagtagctat ttgccttgat gatgaagacc aaaaccttga attaacacta
    20761 aatagtctca tttcagctgg tgattttatc aagtcaaaat ggactacaaa ccattttcaa
    20821 atgcttgagc acttaatact tcaagataat tatcaagaac aatttcaaca tcaaaagtta
    20881 gcccaactgg aaaatattga acctagtgcg ctgactaaac gccttaaagc aagcggtctg
    20941 aagatttact taagaacgag aacacaggca gccgatctat tagttaaaag ttgcactcaa
    21001 actaaagggg gaagctatga tttctgattt tttaagggat aatccaatcc taactttatt
    21061 attttgtgca cattttttag ctgactttca gtggcaaagt caatccttag cagatagtaa
    21121 atcacatagt tggaggggac tctggagggg actctggaga catctcttga ttgttttcct
    21181 ccccctagca gctttaatga tattgatacc agaaactact ttgttaaatt taagcatatg
    21241 gggaagtcat attgttattg atagtataaa gaaattgagt tacccttggg tagaggaagg
    21301 gcatttccaa aaagcagctt ttattataga ccaactagcc cactatactt gcattatcgt
    21361 tttttaccat gcgcttccta catatttacc gcctaatcat tggcttttgc ctatcaagca
    21421 ctttattgtc atagctctgg tttttatcat tatcaccaaa cccattaata ttgtgtttaa
    21481 aattttcttt aataaatttc aagctaaaga attatcatca ttattaacac aagaaaaaac
    21541 aaaaataatg aaagaaaaat ctgaagatca tgaagaaaca attgaaggag caggagctat
    21601 gattggtaat ttagagcgtc taattatggc tatattacta atctcaggac agtatgctgc
    21661 tataggtctt gtttttactg caaaatccat tgcacgttac gataaaattt ctaaaagtca
    21721 ggtttttgcc gaatattatt taattggatc attgttctca attataagtg ttttgataac
    21781 tcattggtta cttttggtat agaaaagcgc tagaaatatc ctaacgcttt tcttttattc
    21841 tgtcttataa gcataagtaa aatcaacatt caaaccagtt gaatttctta ccaacccctt
    21901 taatttagaa ttttggagtc cttttttccc taggtaataa actgggttat aatatgattg
    21961 atcaaataaa gctttttcag cagttttgta atcattagcg gcatctcctg gtttcaaggc
    22021 gtcagtagta attgcctttt gataggcatt gtcatactct ttagaagaaa atttaccata
    22081 attatacgca gaattagtcg tgaataaccc atagaaagtt gatccttctg gataatctcc
    22141 tccccatgag aagagcacga catcaaaatt ttcatttttt gcatcttcaa ggcgttgctt
    22201 gaaagtaaca aatttttctt caacagttag accaggcaaa gcactctccc atgtagattt
    22261 cacatagtca acagcatttt tagctgctgg agagtccgaa tcagctgtga ttgttaactt
    22321 gagagatgac tgaccaacct cagcaagacc ttctttaaaa agtttttgag ctttacttgt
    22381 attatacgta taacctgggg cagtatactt ggaaagatcc tcaccattag gtaatttggc
    22441 taatttttta gggaccagag attcagcagg agttgaacct gtatcaactg ccgctttaac
    22501 aacaccttta cgatcagtcg ctaaattcaa agcttgccta attttctgat ttgtcaatgc
    22561 tttaaccgaa ccagtttggt tataaataat atatgtagta cgtgcatcag acgcatctac
    22621 tacatcttta ttatttttat tagcttgata aatagcagaa gttcctgata tttcagcaaa
    22681 atcaatctga ccttgcttgt acatttgaac tgcagtatct ggttttttga tagtttgaac
    22741 tatgacacta tttgttttaa catgtttaga atcccaataa tatttatttt tgactaattt
    22801 aaatttacca tttgaaccat tccaatcctt aacaaggtaa ggacctgagt aaatttgatt
    22861 ttttgatgtt gtaccataat ctttaccaac tttttcaaca tacgattgtt tttgaggcat
    22921 aaaattagag aaggctaaat aatatttaaa ttgagggcat ggacttgtca atttaaaggt
    22981 cacttgattg cctttagctg ttacaccaag tttatttaaa tctttaatat ctccagaatt
    23041 aatcttatca gcatttaaaa ggtgcgattc tgtcgccaag taagcatatt cagatgcagt
    23101 tttaggatca actattctcc gccatgtgta aacaaaatct tcagcgctta gtttgcttcc
    23161 atctgaccat tttaaattat cacgaagtgt agcagtataa gtaagaccat cagaagaaac
    23221 ggatactttt ttagctaaat cgggttttgg tttgccctct ttatcaattc taagtaaatt
    23281 gctgcctgaa ttacctatag ctaagtttga ataagcatct gtattctttg aaatgtccaa
    23341 tgtactgatt tcagtaggaa tataccaatt aataacttta ttatctccag agttctttga
    23401 ctcattacca caggcaacca gggtaactgt ggtggctgtt agagtcgcca aagatatacc
    23461 caaaaactta agtttttttc tattaaaact agacataata c
//
LOCUS       DATRSD010000026        23396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRSD010000026 DATRSD010000000
VERSION     DATRSD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.026"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..468
                     /locus_tag="VBD85_000818"
     CDS             <1..468
                     /locus_tag="VBD85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO7133383.1"
                     /translation="CWLCAGTLRNFIWNKLSGINETLTSDIDVVFFDKNISYEETVAL
                     EQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTSSKDAISKFPEKCTAVGARLDDRNQL
                     ELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYLKRVDKKKWNNIWPRLTIIKK"
     gene            688..1107
                     /locus_tag="VBD85_000819"
     CDS             688..1107
                     /locus_tag="VBD85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO7133384.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1307..1786
                     /locus_tag="VBD85_000820"
     CDS             1307..1786
                     /locus_tag="VBD85_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7133385.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLISGERFAGKKQFIRDIQPFFNAFKAD"
     gene            1908..2600
                     /locus_tag="VBD85_000821"
     CDS             1908..2600
                     /locus_tag="VBD85_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7133386.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGLG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2597..3349
                     /locus_tag="VBD85_000822"
     CDS             2597..3349
                     /locus_tag="VBD85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO7133387.1"
                     /translation="MKKNKVIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTKEQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMAKHHLTLEEYITLLKENNQ"
     gene            3346..3921
                     /locus_tag="VBD85_000823"
     CDS             3346..3921
                     /locus_tag="VBD85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO7133388.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4016..5098
                     /gene="hrcA"
                     /locus_tag="VBD85_000824"
     CDS             4016..5098
                     /gene="hrcA"
                     /locus_tag="VBD85_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO7133389.1"
                     /translation="MCKSANFKKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYYVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QSLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLATKLGDYYRYLNSNHYEVH"
     gene            5101..5673
                     /gene="grpE"
                     /locus_tag="VBD85_000825"
     CDS             5101..5673
                     /gene="grpE"
                     /locus_tag="VBD85_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO7133390.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5854..7683
                     /gene="dnaK"
                     /locus_tag="VBD85_000826"
     CDS             5854..7683
                     /gene="dnaK"
                     /locus_tag="VBD85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO7133391.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            7972..9111
                     /gene="dnaJ"
                     /locus_tag="VBD85_000827"
     CDS             7972..9111
                     /gene="dnaJ"
                     /locus_tag="VBD85_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO7133392.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFDGGGFGGFEDIFSS
                     FFGGGGMRNPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGT
                     SPVTCQKCHGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTH
                     KVSVKIPAGVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLD
                     ISFVQAALGDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVN
                     IVTPTKLNDAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9225..10472
                     /locus_tag="VBD85_000828"
     CDS             9225..10472
                     /locus_tag="VBD85_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO7133393.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYHRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETISRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADQPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10543..11319
                     /gene="truA"
                     /locus_tag="VBD85_000829"
     CDS             10543..11319
                     /gene="truA"
                     /locus_tag="VBD85_000829"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO7133394.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIHGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMKEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            11282..12040
                     /locus_tag="VBD85_000830"
     CDS             11282..12040
                     /locus_tag="VBD85_000830"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7133395.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTVM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYTDVITPNLVEAQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12050..12514
                     /locus_tag="VBD85_000831"
     CDS             12050..12514
                     /locus_tag="VBD85_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7133396.1"
                     /translation="MRKEKTNQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12511..13080
                     /locus_tag="VBD85_000832"
     CDS             12511..13080
                     /locus_tag="VBD85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO7133397.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13117..13959)
                     /locus_tag="VBD85_000833"
     CDS             complement(13117..13959)
                     /locus_tag="VBD85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO7133398.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGTVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEKGIDLPTYNFSN
                     NHSR"
     gene            14116..15399
                     /locus_tag="VBD85_000834"
     CDS             14116..15399
                     /locus_tag="VBD85_000834"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO7133399.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            15588..16163
                     /locus_tag="VBD85_000835"
     CDS             15588..16163
                     /locus_tag="VBD85_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO7133400.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEDDEEEEEEEEV"
     gene            16436..18040
                     /locus_tag="VBD85_000836"
     CDS             16436..18040
                     /locus_tag="VBD85_000836"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO7133401.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLKLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            18149..19075
                     /locus_tag="VBD85_000837"
     CDS             18149..19075
                     /locus_tag="VBD85_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133402.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTAVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNSSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSGVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            19122..19207
                     /locus_tag="VBD85_000838"
     tRNA            19122..19207
                     /locus_tag="VBD85_000838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19156..19158,aa:Leu,seq:aag)
     gene            19270..19716
                     /locus_tag="VBD85_000839"
     CDS             19270..19716
                     /locus_tag="VBD85_000839"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO7133403.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAYMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            19878..21242
                     /gene="radA"
                     /locus_tag="VBD85_000840"
     CDS             19878..21242
                     /gene="radA"
                     /locus_tag="VBD85_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO7133404.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFENAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            21378..21875
                     /locus_tag="VBD85_000841"
     CDS             21378..21875
                     /locus_tag="VBD85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO7133405.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTDDVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            22029..23348
                     /locus_tag="VBD85_000842"
     CDS             22029..23348
                     /locus_tag="VBD85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO7133406.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDNKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
BASE COUNT         7750 a         3554 c         4902 g         7190 t
ORIGIN      
        1 tgttggctgt gtgctggcac attgagaaac tttatatgga ataaactttc tggaataaat
       61 gagacattga catcagatat tgatgttgta ttttttgata aaaatatttc ttatgaagaa
      121 acggtggcat tagaacaaca attaaaggat aactatccac agtacgattg ggaacttaaa
      181 aatgaatttt acatgaacac tcattctcct aataccccaa agtatactag ctctaaagat
      241 gctatttcga aatttcctga aaagtgtact gcagtgggag caaggttaga tgatagaaat
      301 caactcgaac tttacctccc ttatggtgaa gaggaaatat taaatttcat tgttagtcct
      361 acgccatatt ttgaagagga tctattacgt tataatgttt atttgaagcg agtggataag
      421 aagaaatgga ataatatttg gcctcgttta acaatcataa aaaaatgata acgttactgg
      481 agttggctta acagctttta aatttttaga ctgacaaatg gtatagacct ttaataaggt
      541 cgtttatcga taatcatagt gtactgtgtt ataatcgtta tagcataaaa acacgttgca
      601 gttggtagtc tgcaagcgtt cagtgaacgt cagtaacctt ccctccaggt cgtccattgc
      661 aatttaatat ttaaaggagg ggactgtatg aaaatgacag tctattttga tggtaatttt
      721 tggttgggtt tgattgagta cgatgatgat ggtgattaca aagtttttcg ttattttttt
      781 ggaaaagaac ctaaagatga tgatgttttt aattttataa atcataaact taatgatttg
      841 ataaaaaaat atgagtttgt taaaacggat atttctctaa agcgtacgaa tgaacacaaa
      901 aaaagcccta aaagaatgca aagagaaatt aatagagaaa aacgtaagcc agtagtatct
      961 acaaaagcgc aattggcgat gaaaactatt catatgagta taaaaaacga acgtcaatta
     1021 agtcagaaat gtaagaaaaa tgagttaaga aagcatcggt accaattaaa acaagaaaaa
     1081 cgttaccaaa agaaaaaagg gcattgattg tgtcatcata taaaaagctt gagatgatac
     1141 tctcaagctt tttatatgtt aactaatatg agtcactaaa tagtctgata atgctcaatt
     1201 aatgtgtttt gggaaaaaat catttcctta aaggacacca ttgtcatgtt gttatcacct
     1261 tacttttttt atacttagta tatgtttaag aaaggaataa gttgttttga gagattttga
     1321 ggagttgttt gataaagtaa agccaatcgt aatgaagctg agaagaaatt attttgttca
     1381 actttgggaa tatgatgatt ggattcaaga aggacgtatt gtacttttta ggttgcttga
     1441 agagcatccg tatttattgg ataatgaaag caaactattc atttacttta aaacaaaatt
     1501 ttcaaattat ttaaacgatg tattaagaca ccaagattgt caaaagcgac aatttaataa
     1561 aatgccttat gaagaaatca gtgaagttag tcactatgtt aagagtaaag gactagtttt
     1621 agatgactat attgcttatc gtgatacttt aacaaaagtg gaagaaactt tgtcagatat
     1681 tgataaagaa aaatttgaaa aactgattag tggtgaacgt tttgcgggga agaaacaatt
     1741 catacgagat attcaacctt ttttcaatgc ttttaaagcg gattaatcga ttttatttct
     1801 gcagtagata gctgcagaat ttttttataa ggaaagttcg ctgatatatt cctgtgaaag
     1861 cctttgtttt ttgttataat ataataaaat gcttgtgagg tgtcagtatg agtaaagttc
     1921 gtttatatat cgctcgtcat ggtaaaacaa tgtttaatac aattggtcgc gctcaggggt
     1981 ggagtgatac tcctttaact acctttggag aactcgggat taaagagctt ggtttgggac
     2041 taaaggcttc aaatattagt tttaaagaag ctttttctag tgatagtgga cgtactttac
     2101 aaactatgga aattattctc agggaagtgc aacaagaaaa tattccctat acgagagata
     2161 aacgaatccg cgagtggtgc tttggtagtt tggatggagg atatgatggc gaccttttta
     2221 atggcgttct gcctcgagtt tcaaacggag atatgagcca tttgacacac gaagaaattg
     2281 ctaatttgat ttgtcaagtt gatacggctg gctgggcaga accgtgggca atattgagta
     2341 accgtattct gtcaggcttc actgctatag ctaaaaaaat agaggatatt gggggaggta
     2401 atgcgattgt tgtcagtcat gggatgacta ttgcgacttt tttatggtta atcgaccata
     2461 gtacccctcg tagtttaggc ttagacaatg gtagtgtctc agtcgttgat tttgaagatg
     2521 gaacattttc tattcaatct attggggata tgtcctatcg agaaaaagga agagaaattt
     2581 tagaaaaaac attacaatga aaaaaaataa agttatccga ttcagtttag ttggtgttct
     2641 acttgcgata ctatgcttta gtctttttgc tttattgaag cctaacagtc aacaatcatc
     2701 atctcaaaag ttgaggaatg aggatataaa aaagacatcc tctcaaaaaa gaaataagaa
     2761 attacgatta ccagctgtat catcaaaaga ttggaacttg attttggtca atcgtgacca
     2821 taaacatgaa gaattaagtc cagatgtggt gcctgttgaa aatatttatt tggataaacg
     2881 tattacgaag caagctactc agtttttaga ggctgctaga gcaattgatt cacgagaaca
     2941 tttaatttcg ggttatcgta gtgttgccta tcaggagaag ttgttcaatt cttatgttac
     3001 tcaagaaatg actagtaacc ctaatttgac gaaggaacaa gcagaaaagt tggtaaaaac
     3061 ttactctcag cctgcaggtg ctagtgaaca ccagactgga ttagcgatgg atatgagtac
     3121 tgtagattct ttgaatgaga gcgatcctag agtagtcagt cagttgaaaa agatagctcc
     3181 acaatatggt tttgtcttac ggtttccgga tggtaaaaca gcagaaacag gggtaggtta
     3241 tgaagattgg cattaccgct atgttggggt agagtctgca aaatatatgg ccaaacatca
     3301 tttaacatta gaagaataca taactttatt aaaggagaat aaccaatgag gaaacgtttt
     3361 tccttgctaa attttattgt tgttactttt attttctttt tctttattct ttttccgctt
     3421 ttaaaccata agggaaaagt agatgctaat tctaggcaga gtgttaccta caccaaagaa
     3481 gaatttatac aaaaaattgt gccagatgcg caagatctag gaaagtcgta cggtattcgt
     3541 ccttcattta ttattgcaca ggcggctttg gattctgatt tcggagagaa aatattagct
     3601 aataagtatc ataatctgtt tggtttgctt gcagaaccag gaacgccctc aattacctta
     3661 aatgatagta gtacaggaaa aaaacaggaa aagcaattta ctcattataa atcttggaag
     3721 tattcaatgt atgattacct tgctcatata aaatctggag cgacaggcaa aaaagattcg
     3781 tatactataa tggtgtctgt taaaaatcca aaaactttag tgcaaaaatt acaagatagt
     3841 ggttttgata atgacaaaaa gtacgctaaa aaaatgacgg aaatcattga tttgtatgat
     3901 ttaacaagat atgataagtg atattagcac tctttgaata agagtgctaa tttttgatgt
     3961 tttttccttg actatctttt aaaaaggagt ataataaaat catagttagc agtcgatgtg
     4021 taagagtgct aatttcaaaa agaccgagag gggtgatgtt tttgtgatta cacaacgtca
     4081 aaatgatatt ttgaatttaa tcgttgagtt atttacacaa actcatgaac cagtagggtc
     4141 taaagcttta cagagaacga ttgattctag tagcgccact attcgtaatg atatggcaaa
     4201 actagaaaag ttggggttat tggaaaaagc gcacacttct agtggacgga tgccgagtcc
     4261 agctggtttt aagtattttg tagagcactc ccttagacta gatagtattg atgagcaaga
     4321 tatttattat gtcataaaag cttttgattt tgaagcattc aaattagaag acatgcttca
     4381 aaaagctagt catattttat cggagatgac gggctataca tccgttattt tagatgtgga
     4441 gccagctcgt caacgtttaa cagggtttga tgttgttcag cttagcaacc atgatgctct
     4501 agcagtcatg actttagatg aatctaagcc agtgacagtg cagtttgcta ttccaagaaa
     4561 ctttttgaca cgtgacttga tagcatttaa agctatcgta gaggagcgct tgctggatgg
     4621 tagtgtcatg gatattcatt ataagttgag gacagaaatt cctcagatcg ttcagaagta
     4681 ttttgtaact acggataatg tgttacaact atttgattat gttttttcag agctgtttct
     4741 ggaaacggta tttgtagctg gcaaggtaaa ttctttaacg tattcagatc tatcaacgta
     4801 tcagtttttg gataatgagc agcaggtagc gatttctttg aggcaaagtt tgaaagaggg
     4861 tgagatggct tctgtccaag tggcagatag tcaagaagcc gcacttgcag atgtgtcagt
     4921 tttaactcac aaatttttga ttccttaccg tggatttgga ctcctgagtt tgattggtcc
     4981 tattgatatg gattatcgta ggtctgttag tttggtaaac attatcggaa aggtattggc
     5041 tactaaatta ggtgattatt atcgatacct aaattctaac cattatgaag ttcactgata
     5101 atggcagtat tcaataagtt atttaaaagg aggcactcag tgtcagaaga aattaaaaaa
     5161 gacgatttac aggaagaagt ggaagccact gaaactgaag agacagttga ggaagttatt
     5221 gaagaaattc ctgaaaaatc agagttagaa ctcgcaaacg agcgtgctga tgagtttgaa
     5281 aataaatact tgcgtgctca tgcagaaatg cagaatatac agcgtcgctc tagtgaagaa
     5341 cgtcagcaac ttcaacgcta tcgttctcag gatctggcaa aagcaatctt accatcgctt
     5401 gacaatctag aacgcgcctt ggctgttgaa gggttgacag atgatgttaa aaaaggttta
     5461 gaaatgactc gtgatagcct tattcaagca ctaaaggaag aaggcgtaga agaagtagaa
     5521 gtcgactcat ttgaccacaa ctttcatatg gcagttcaga cccttccagc cgatgatgaa
     5581 catccagccg atagcattgc agaagtcttt caaaagggat ataaattgca cgagcgtctg
     5641 ctaagaccag cgatggtagt tgtctataac taaggtaaat gagttttcgt ttttgtccgt
     5701 aatgacagta aactagatag caagttagaa gctattcagc ttgctgatta aactatagtg
     5761 attgcttaga attggaagta aaataattcg agtgcttact aagataaatt gaaataaaaa
     5821 gtaataaagt attataaaat aagaggtatt aacatgtcta aaattattgg tattgactta
     5881 ggtacaacaa actcagcagt agcagttctt gaagggactg aatcaaaaat cattgctaac
     5941 ccagaaggca atcgtacaac tccttcagta gtatcattca aaaatggtga aattatcgtg
     6001 ggtgatgctg caaaacgtca agcggtaaca aatccagata ctgttatctc tatcaaatca
     6061 aagatgggaa cttctgaaaa agtttctgca aatggtaaag aatatactcc tcaagaaatt
     6121 tcagcaatga ttcttcaata ccttaaaggt tatgctgaag actatcttgg agaaaaagta
     6181 gaaaaagcag ttattactgt tccagcttac ttcaacgatg cacaacgtca ggcaactaaa
     6241 gacgctggta aaattgcagg tcttgaagta gaacgtatcg ttaacgaacc aacagcagcc
     6301 gcacttgctt atggtatgga caagactgac aaggatgaaa aaatcttagt ttttgacctt
     6361 ggtggtggta catttgacgt atcaatcctt gaattaggtg atggtgtctt cgacgttctt
     6421 gcaacagcag gtgataacaa acttggtggt gacgactttg accagaaaat tattgatttc
     6481 ttggtagaag aattcaagaa agaaaatggt attgatcttt ctcaagacaa aatggctctt
     6541 caacgcttga aagatgctgc tgaaaaagct aaaaaagacc tttcaggtgt aactcaaact
     6601 caaatttcat taccgttcat cactgctggt tctgctggtc ctcttcactt ggagatgagc
     6661 ttatcacgtg ctaaatttga cgatctcact cgtgaccttg ttgaacgtac gaaaactcca
     6721 gttcgtcaag ctctttcaga tgcaggcttg tcattgtcag aaattgatga agttatcctc
     6781 gttggtggat caacacgtat cccagcagtt gttgaagctg taaaagctga aactggtaaa
     6841 gaaccaaaca aatcagttaa ccctgatgaa gtggttgcta tgggtgctgc tatccaaggt
     6901 ggtgttatca ctggggatgt gaaagacgtt gttcttcttg acgttacacc attgtcactt
     6961 ggtattgaaa caatgggtgg tgtcttcact aaattgatcg accgcaacac aactatccca
     7021 acatctaaat cacaagtctt ctcaacagca gcagacaacc aaccagccgt tgatatccac
     7081 gttcttcaag gtgaacgccc aatggcagca gataacaaaa cactcggtcg cttccaattg
     7141 actgatatcc cagctgcacc tcgtggaatc ccacaaattg aagtaacatt tgatatcgat
     7201 aaaaatggta ttgtatctgt taaagctaaa gatctcggta ctcaaaaaga acaacacatt
     7261 gttatccaat ctaattcagg attaactgat gaagaaattg ataaaatgat gaaagatgct
     7321 gaagcaaatg ctgaagcaga tgcaaaacgt aaagaagaag ttgatcttaa aaatgaagtt
     7381 gaccaagcca tctttgcaac agaaaaaact attaaagaaa ctgaaggcaa aggctttgat
     7441 acagaacgcg atgcagcgca atcagcactt gatgagttga aaaaagctca agaatcaggt
     7501 aaccttgacg acatgaaagc taaacttgaa gctcttaacg aaaaagcaca agctcttgca
     7561 gttaaacttt acgaacaagc ggctgcagca caacaagcag ctcaaggggc tgaaggtgca
     7621 caatcagctg attcatcaag caagggtgat gatgttgtag atggcgaatt cactgagaaa
     7681 taattattaa tattgttcag attcatttga atataagcat gaaaactata ctagcatagt
     7741 aaagttcttc gtgatagagg ttgctcaata atctagataa gtttcagatt acataagcta
     7801 attttgctat cactaaataa aaacatatta ataataaata ggcggggcgc ctcgctccgt
     7861 ctgttttatt aagtgtcata tatatgttaa ctatttagag ttgtaactga gcaagaataa
     7921 ttgttaatct cttcaagagt agtatatgaa caaaatataa aggattagat aatgaacaat
     7981 acagaatttt atgatcgtct tggcgtttca aaagatgctt ctcaggacga aataaaaaaa
     8041 gcttaccgta ggatgtctaa gaaataccat ccagatatta ataaggaagc gggagcagag
     8101 gaaaaatata aagaggttca agaagcttat gaaaccttga gtgatacaca aaaacgtgct
     8161 gcctatgatc aatatggcgc tgctggggca aatggcggtt ttggtggctt cgatggtggc
     8221 ggttttggtg gcttcgatgg tggtggcttt ggtggttttg aagacatctt ctcaagcttt
     8281 tttggtggag gagggatgcg caatcctaat gcaccacgtc aaggagatga cctacaatat
     8341 cgtgttaatt taagttttga agaagctatc tttggagctg aaaaagaagt atcttataat
     8401 cgggagtcat catgtcatac ttgttcagga agtggagcta agccaggtac tagccctgtg
     8461 acttgtcaaa agtgtcatgg ttcgggtgtg attaatgttg atacacaaac acctctagga
     8521 acaatgcgtc gccaagtgac ttgtgatgtt tgtcagggta gtggtcaaga aatcaaagag
     8581 aaatgtccaa catgtcatgg aactggtcat gagaagaaga cgcataaagt atcagttaaa
     8641 atacctgcag gagttgaaac tgggcaacaa attcgtttaa caggacaagg tgaagctggc
     8701 ttcaatggtg ggccttatgg tgatctcttt gttattatta acgttctccc aagccaacag
     8761 tttgagcgta atggatcaac aatttattat actttggata ttagctttgt ccaagctgct
     8821 ttgggtgata caattgatat acctactgtc catggagcgg tagaaatgtc aattcctgct
     8881 ggtactcaga caggaaaaac attccgtctt cgcggaaaag gtgcccctaa acttcgaggg
     8941 ggtggacaag gtgatcagca tgttactgtt aacatcgtaa ctcctactaa attgaatgat
     9001 gctcaaaaag aggcattgca cgcttttgca gaggcaagtg gagataagat ggtacatccg
     9061 aagaagaaag gtttctttga taaagtcaaa gatgctttag atgttgatta aaaaataaaa
     9121 gtctagaatc gaaagtattc tagacttttt tgtattgcta gagggattgc ttgaacctaa
     9181 attttacagt acaatgaatc taatattgta ggggggcaat ttttatggta accaaagttg
     9241 aagaaattcg ttcatatttg atagcttcta tacaaaatgg taagttggct ccaggagatc
     9301 gcctaccatc tataagacag ttagctaatc aattttcctg taacaaagat acagtgcaac
     9361 gagttttgat ggaattgcgt tttgataatt atatctatgc aaagccgagg tcaggctatt
     9421 acgtctttga ttctcatcaa gaggaagttg aagaaggggt tagtttacca aactctgaga
     9481 ttgcaaatat agcttatgat gattttagat tgtgtttgaa tgagaccctt attggtaggg
     9541 aagattacct tttcaattat taccatcgtc aagaaggtct tcttgattta agtaaagcgg
     9601 tggctaaatt aatggaagaa acaggggtct atgttcctct tgatgatatt gttattacag
     9661 ctggtactca acaggcatta tttattttga cacaggttac ctttccaaat cgaaaatctc
     9721 gagttttaat agaagaaccg acctatcctc gtatgattga actaatcaaa acacaaaatt
     9781 taccctatga aactatttct cgaggtactc atggaattga ttttcagcgc ttagaggaga
     9841 ttttccagac acaatcaatt aagttttttt atgttatacc tcgcatgcat aatcctttgg
     9901 gaacatccta taatccggta gagatgaaaa gattaataga gatggcagag aagtatgatg
     9961 tttatattgt ggaagatgac tatatgtctg attttgcaag tcagtcacca ttacattatt
    10021 atgatactca cgggcgtgtt atttatctaa aatctttttc aaaggctatt ttccctgctt
    10081 taagattagc tgcgatttgt ttaccacaag ctttaaaatc aacatttatg gcttacaaga
    10141 agttgatgga ttatgatact aatctgattt tacaaaaagc attagcgctt tatattgaaa
    10201 atggccttta tgctaagaat agtcaatatt tgaaatatcg ttatcagaaa gaccttgcaa
    10261 attcaaaatc tattttagct gatcagccta atctaccccc atatagttta catcacgata
    10321 gtgtattatt tgattgttcg aaactcgata acttcaaaat attacggcaa tacggcgata
    10381 ctttggaaaa ttatttttgt caaaaatcgc atcaatctct cttacaagta aaaaatgatt
    10441 cctgcttaaa gcagttcttg ggatcgttgt agtataattg tagggggaca gtttaaaata
    10501 gatattatag ttttcataaa tgaaaggtgg taaaataaac ccatgacaag gtataaggca
    10561 cagatttctt atgatggttc agctttttca ggcttccaaa gacaaccaaa ttgtaggaca
    10621 gttcaagaag aaattgagag aactcttaag cgcctaaata gcgggaacga tgtgataatc
    10681 catggtgctg gtcgcacaga tgctggtgtt catgcttatg gacaagttat tcattttgat
    10741 ttaccacagg cgcgtgatgt agaaaaatta cgctttggtt tagatacaca gtgtccagac
    10801 gatattgata ttgtcaaagt agagcaagtg tctgatgatt ttcattgtcg atatgataaa
    10861 cacattaaaa cttatgagtt tttagtagat atcggtaggc cgaaaaatcc tatgatgaga
    10921 aactatgcga cacattaccc ttatcctgtc attattgagc ttatgaaaga agctattaaa
    10981 gatttagtag gtacccatga ctttacaggc tttactgctt caggtacgag tgtagaaaat
    11041 aaagtacgta ctatttttga tgcgaaaatt cagtttgaag cgtctaaaaa tcttttaatt
    11101 tttaccttta caggaaatgg ttttttgtat aaacaggtgc gtaatatggt tggtaccctg
    11161 ctaaaaattg gtaatggacg catgcctatt agccaaatca aaacaatttt acaggctaaa
    11221 aatcgggatt tagcaggtcc aactgcggca ggaaatggtt tatatttgaa ggagattatt
    11281 tatgaagacg aggaatgttt tagcaatttc aggaaatgat attttctctg gcggcggctt
    11341 gcatgcagat ttagcaactt acgttgttaa taaattacat ggttttgtgg cagtaacatg
    11401 cttaacagta atgtcagata aagggtttga agtcattcct atagaggcat ctatactaaa
    11461 gcagcaacta gaaagtttaa aagatgtaga gtttgggtct attaaactag gattgcttcc
    11521 caatgtagaa acagcacaag ttgttttgga atttgtaaaa tcaaaacaag aatgtccagt
    11581 agtcttagac ccagtcttag tttgtaaaga aaatcatgat ttagaagtta gccaattacg
    11641 tgagcagcta attgcgtttt tcccatatac tgatgtgatt actcctaatt tagtagaggc
    11701 tcaattattg acgggtcttt caattgaaaa cttagatcaa atgaaaattg ctgctgagaa
    11761 actttacgat atgggggcta aacatgttgt tatcaaaggg ggcaaccgtt taaatgctga
    11821 agaggctaca gacctttact atgatggtga acgttttgaa acctatgttt ttcctgtagt
    11881 agatgccaat aatacaggtg ctggatgtac atttgcgtca agtattgcca gccaattagc
    11941 tatgggtaaa aatgtagaag atgcagtcaa aatgtctaaa ggttttgtct atcaagctat
    12001 taaagcatcg gataagtacg gagttgtcca acatttttag gagattttaa tgaggaaaga
    12061 gaaaactaat caattggttc aattggcaat tgtaacagct atatctattg tattggggat
    12121 gtttataagt attccgacac caacaggttt tttgacatta ctagatgctg gaatattctt
    12181 tgctgccttt tactttggca aaaaagaagg ggctgtggta ggtgccttag ctggtttttt
    12241 aattgattta ttgaaaggtt atcccaattg gatgttcttt agtttattga ttcatggggc
    12301 gcaagggtat ttagcgggtt taccaggtag aagacgtctt ttaggtctta ttagtgcaac
    12361 tcttgttatg gtattaggtt atgctatagc tagtggattg atgtatggtt ggggagctgt
    12421 tctaccagat attcctggca atattatgca aaatatggtt ggtatggtcg ttggttttgc
    12481 tcttaataag tctctagaaa gagtgaagaa atgatactag attataataa gttaaagcag
    12541 gaaactaaag ctattgtagt tgatattatt gagcgtagcg ctcttaaaaa aggtcaaatt
    12601 tttgtactag gtctatcatc tagtgaggtt tctggtggtt taattggaaa aaattctagt
    12661 tctgaaattg gtgaaattat cgttgaagtt atcctcaaag agttacattc acgcggcatc
    12721 tatttagcag ttcaaggttg tgaacatgtt aatagggcgt tggttgttga agcagaattg
    12781 gctgaacgtc aacagttgga agtcgttaat gttgttccta atctacatgc tggcggtagt
    12841 ggtcaggttg cagcttttaa gttgatgaca tcaccagttg aggttgaaga aattgtggca
    12901 catgctggaa ttgatattgg agatacttct attggaatgc atatcaagag agtacaagtt
    12961 cccttgattc caattagtcg tgagttggga ggagcacacg ttacagcact agctagtcgt
    13021 cctaaattaa ttggcggtgc tagagcagga tatacgagcg atccaatccg taaattttaa
    13081 aataaacctc tgctatgcag aggtttattg tttatctcaa cgagagtggt tattggagaa
    13141 attgtatgtt ggtaagtcaa tacctttttc aagaatagct ttttgataaa gtttgtaaaa
    13201 ttcagcatag atgtcaaact gagcaccgtt ttgcgtgaag atattaatac gataaacaaa
    13261 ttgaccatta gcattggttg taggaccaaa aacagtaggc tctccaacga tagcagggtg
    13321 ttttgaaaca tactcttcat tgatttttgt tacaatatca gaaatctgat caaggtttgt
    13381 atgaacaaat aaaggaatat caatttgagc acgcatattg ctacgagact tattgctgac
    13441 aattgtaata ttacgattag gaatgaagtg taaggttcca tcgaaatcat gaatttgtgt
    13501 ggtgcgtatc ccaacattag taacagtgcc tgatacagta ccaacattga taatatcacc
    13561 aacatcaaat tggttttcta gcagaataaa aaagccattg acaacatctg ataagaagcc
    13621 ttgagcacca agtccaatag caaccccagc aataccagcc ccagctaata aactagaaat
    13681 tggaacgccc aatatactta aaatccaata gaatagaaag aaataaaggg tatagtttaa
    13741 aacattgtga gaaagtttag ctaatgtttt ttgacgagct actttttgtc tagaaaaggc
    13801 gagtgagcga ttaacagtct tttcgaaaag ataattgatg acttgcctaa cgataacgaa
    13861 tgcaatgatc aataataaga ttgatattag tttggtgaag aaagtaaata aaacttcctc
    13921 tacattaaga tggtctataa atttttctaa agtaatcata gtaatagtct agtaaaaaaa
    13981 aagccataga gcaagtattt taaaagctta tcgttaagca gtttaaagct tcttttttgc
    14041 ataaaaactt tgtaaaacgt tttaaattat ggtaaactaa actgtatcta aataatttta
    14101 aggagaaatg acttaatgtc tacatcattt gaaaataaag ctacaaaccg tggtattatc
    14161 acatttacaa ttagtcaaga tgagatcaag ccagctcttg atcaagcatt caacaaagtt
    14221 aaaaaagatt tgaacgttcc aggattccgt aaaggacata tgcctcgtac agttttcaac
    14281 caaaaatttg gtgaagaagc actctacgaa aatgcattga atttagtgtt gccaaaggct
    14341 tacgaagcag ctgttgcaga acttggttta gacgttgttg ctcaaccaaa aattgatgtt
    14401 gtatcaatgg aaaaaggtca agattggaag ttgacagctg aagttgttac aaaaccagaa
    14461 gttaaacttg gtgattacaa agacctttca gtagaagttg atgcttcaaa agaagtttca
    14521 gatgaagaag ttgatgctaa agttgaacgt gagcgtaaca accttgcaga attgactgtt
    14581 aaagatggtg aagcagcaca aggtgataca gttgttattg actttgtagg ttcagttgat
    14641 ggtgttgaat ttgatggtgg taaaggagat aacttctcac ttgaacttgg ttcaggtcaa
    14701 tttatcccag gatttgaaga acaattagta ggttctaaag ctggtcaaac tgtagacgtt
    14761 aatgtaacat tcccagaaga ttatcaagca gaagatcttg ctggtaaaga tgctaaattt
    14821 gttacaacaa ttcatgaagt aaaaactaaa gaagttccag cacttgatga tgaacttgca
    14881 aaagatattg atgaagaagt tgaaacactt gatgagttga aagcaaaata tcgcaaagag
    14941 ctagaatcag caaaagaaat tgcatttgat gatgcagttg aaggtgcagc aatcgaatta
    15001 gctgttgcca atgcagaaat tgttgaatta ccagaagaga tggtccatga tgaagtacat
    15061 cgtgctatga atgagttcat gggtaatatg caacgtcaag gaatttcacc agaaatgtac
    15121 ttccaattaa caggtacaac tgaagaagac cttcataagc aataccaagc agatgctgac
    15181 aaacgcgtta aaactaacct tgttattgaa gcaattgcag ctgcagaagg ctttgaagca
    15241 acagatgaag aaatcgaaaa agaaatcaca gatcttgctt cagaatataa tatggaagct
    15301 gaccaagtac gcggtctact ttcagcagat atgcttaaac atgatattgc aatgaaaaaa
    15361 gcggttgacg ttatcactag ctcagctact gtaaaataat tgcttattaa attttgaaaa
    15421 gattgggctt cccagtcttt ttatttgtga ttattctttt gacgaatagc taaaaatagc
    15481 gtaaaatagt aaatagcgaa aatttgaaaa tatgacttat ctattttcta tctataaaga
    15541 atattgctaa acaaaatgat agtcttacac aattaaggag aataactttg gaattagaag
    15601 tatttgctgg gcaagaaaaa agtgaacttt cgatgattga agttgctcgc gctattttag
    15661 agcaacgtgg tcgtgataac gaaatgtact tcagcgattt ggtaaatgat attcaaacct
    15721 accttggaaa atctgactca gcaatccgtg agtcattgcc atttttctat tcagatttga
    15781 atacagacgg aagttttatt ccacttggcg aaaacaaatg gggacttcgt tcatggtatg
    15841 ctatcgatga aattgacgag gaaatcatca ctcttgaaga agatgaagat ggcgctccaa
    15901 aacgtaagaa gaaacgtgtc aatgctttca tggatggtga tgaagatgct attgattaca
    15961 atgatgatga tccagaagat gaagatttca cagaagaaac accaagttta gaatatgatg
    16021 aagaaaaccc tgacgatgaa aaatcagagg tagaatcgta tgattctgag attaatgaaa
    16081 tcattcctga tgaagattta gatgaggatg tagaaatcaa cgaagaagac gatgaagagg
    16141 aagaagagga agaagaggtt tagacaggga ttttgtattt gacaaatagt cagatatagt
    16201 ttaaactatt attcgggcac ctcttatgag gtcagataaa gctccctagt gatagggggc
    16261 tattttgttt ttgggaaaga tacagaatgg ttccagatag ttttctgagt aggcccttga
    16321 tcagatagtt tctactagtt ttgactagag aagctggtat tatttatcaa tcatgtgttt
    16381 tgggttttac ccgtaaacta actgttagat taacatttaa tttaaggagt ttttaatgac
    16441 aaagtatatt tttgttacag gtggtgtggt ctcttctatt ggtaagggga ttgttgcagc
    16501 aagtcttggg cgcttactaa aaaaccgtgg gctaaaggtg acaattcaaa aatttgatcc
    16561 atatattaat attgacccag ggacaatgag cccttatcaa catggagaag tttatgttac
    16621 agatgatggt gcagaaactg accttgacct tggacactac gagcgtttta ttgatattaa
    16681 cttaaacaaa tattcaaatg ttacaacggg taagatttat agtgaagttc ttaaaaaaga
    16741 acgccgtgga gaataccttg gtgcaactgt tcaagttatc ccacacgtta cagatgcttt
    16801 gaaagaaaaa attaaacgtg ctgcgacaac tacagattca gatgttatta ttactgaagt
    16861 tggcggtact gttggagata ttgagagcct tccgttttta gaagcacttc gtcaaatgaa
    16921 ggcagatgtt ggttctgata atgttatgta cattcataca acattattac catacctaaa
    16981 ggctgccggc gaaatgaaaa caaaaccgac tcaacattct gttaaagagt tacgtggctt
    17041 aggtatccaa cctaatatgc ttgttatccg tacagagcaa ccagcaggtc aaagtattaa
    17101 gaataaattg gcacaatttt gtgatgttgc accagaggct gttatagagt cccttgatgt
    17161 tgaccatatt tatcaaattc cacttaatat gcaagctcaa aatatggatc aaattgtttg
    17221 tgatcacttg aagcttgaga cacctgctgc tgatatgaca gaatggtcag caatggttga
    17281 taaagtaatg aaccttgaga aaaaagttaa aattgcattg gttggtaaat atgttgaatt
    17341 acctgatgcc tacctttcag ttgtggaagc ccttaagcac tcagggtacg tcaacgatgt
    17401 tgctattgat ctaaaatggg taaatgctgc tgaagtgaca gaagataata ttaaagaact
    17461 tgtcggagat gcagatggta ttatcgttcc tggtggattt ggccaaagag gtagtgaggg
    17521 taaaattgaa gcaattcgct atgcacgtga aaatgatgtt ccaatgcttg gtgtttgtct
    17581 cggaatgcag ctaacttgtg tcgaatttgc gcgtaatgtt ttaaaacttc atggagctaa
    17641 ttcagcagag cttgacccta aaacaccgtt cccaatcatt gatattatgc gtgatcaaat
    17701 cgatattgaa gatatgggtg gtactcttcg ccttggtcta tatccatgta aattgaaagc
    17761 aggttcaaga gctgcggcag cttataataa tcaagaagtt gtccaacgtc gtcaccgtca
    17821 ccgttatgaa tttaatacaa aattccgtga acaatttgag gctgctggat ttgtcttctc
    17881 aggggtatca ccggataatc gtttgatgga agttgttgag ttaccagaga aaaaattctt
    17941 tgttgcggca caataccatc cagaattaca atcacgtcct aaccatgctg aagagcttta
    18001 cacagctttt gtaacagcag cagttgaaaa tatgaaataa ctttatcgcc tcccagtatt
    18061 aggaggcgat tttatttttt tagatagttt cactatcaaa gtatcattag ctcttttctt
    18121 tttctaaaaa taaggtataa taggttgcat gaaaaagatt cgattatcaa agtttattaa
    18181 aatgattgtt gttattttgt ttttaattag tgtagcagct agtttttatt ttttccacgt
    18241 tgcccaagtt cgagatgata aatcctttat ttcaaatggt caacgtaagc ctggaaactc
    18301 tttatatgct tatgataaat cctttgataa gctattaaag caaaaaatag aaatgacaaa
    18361 ccaaaatata aagcaagttg cttggtatgt tcctgctgct aagaaaactc ataagacagc
    18421 tgttgtcgtt catggttttg cgaatagcaa agagaatatg aaggcatatg gttggctgtt
    18481 tcataagtta ggatacaatg ttcttatgcc tgacaacatt gcacatggtg aaagtcatgg
    18541 gcagttgata ggctatggct ggaacgaccg cgagaacatt atcaaatgga cagaaatgat
    18601 agttgataag aattcatcaa gccaaattac tttatttggt gtttcaatgg gtggagcaac
    18661 agtcatgatg gctagtggtg aaaaattacc tagtcaggtt gttaatatca ttgaagattg
    18721 cggttattct ggtgtttggg atgaattaaa atttcaggct aaagagatgt atggtttacc
    18781 agccttccca ctcttatatg aagtttcaac aatttctaaa atcagagcag gtttttcgta
    18841 tggacaagca agtagtgtcg aacaattgaa aaagaataat ttaccagccc tctttattca
    18901 tggtgataag gataattttg ttccaacaag tatggtttat gacaactata aagctacagc
    18961 aggtaagaaa gagctttata ttgtaaaagg ggcaaaacat gcgaaatctt ttgaaacaga
    19021 gccagaaaaa tatgagaaac gtatctctag ttttttgaaa aaatatgaaa aataatattg
    19081 acaacaatat gataagttga tataatttat tatgttgtta agcggggatg gcggaattgg
    19141 cagacgcgca agactaagga tcttgtgacc gcttttggtc gtgagggttc aagtccctct
    19201 ctccgcagac tagtcgagtt catttggaac tcgacttttt gcatatttaa gataaagatg
    19261 gagtatcaca tgtcaaaagt acgtggcttt gaattagtta gccaatttag caataaagag
    19321 ttattaccaa aacgagaaac agctcacgca gctggctatg atttaaaagt agctaaaaaa
    19381 acggttattg aacctggtga aattacattg gtcccgaccg gtattaaagc atacatgcaa
    19441 ccaggggagg ttctgtacct ttatgatcgt tcatcgaatc cacgtaaaaa agggattgtc
    19501 ctaataaatt cagttggtgt tattgatggt gactactata ataaccaagt caatgaagga
    19561 catatttttg cacagatgca aaatattact gatcaggctg ttattttaga agaaggggaa
    19621 cggattgttc aggctgtttt tgccccattc ttattggcag atgacgatca ggctacagga
    19681 atgcgtacag gtggttttgg tagtactgga aaataaaatt attttacgaa taatgttaga
    19741 aaaggctaga cttacttctt agcctttttt gatgagagta aacttatatt ttctctaact
    19801 tggtgtgata taatgagtgt actaattatt tggtaccaag ctatcatact aatttagaag
    19861 ttataaagga ggcctgagtg gctaagaaaa aatcagtctt cacctgtcaa gagtgtggtt
    19921 accaatcacc taagtatttg ggacgttgtc caaattgctc agcctggtct tcatttgtag
    19981 aagaggttga agttcaagaa gttaaaaatg cacgagttag tttgaacggt gaaaagtccc
    20041 gaccgacgaa gttaaaagat gtttcctcaa ttaactattc tagaactaag actgatatgg
    20101 atgaatttaa ccgtgttcta ggaggcggtg ttgtaccagg aagtttggtt ctgattggag
    20161 gagaccctgg gataggtaaa tctactttat tactacaagt atcaactcag ctagctaata
    20221 aaggtacagt attatatgtc tcaggtgagg aatctgcaga acagattaag ttacgaagtg
    20281 agcgtctagg cgatatcgat aatgaatttt acctttatgc cgagactaat atgcaaagta
    20341 ttcggtcgga gattgagaag attaaaccag attttcttat tattgattct atacaaacta
    20401 ttatgagtcc cgaagtatca agtgtccaag gctctgttag ccaagttcgc gaagtgaccg
    20461 ctgagttgat gcagttagct aaaactaata atattgctac ttttattgta ggtcatgtga
    20521 ctaaggaggg aacattagca ggccctcgaa tgctagaaca tatggtggat acggttcttt
    20581 attttgaagg ggagcgtcat cacacgttcc gtattttacg ggcagtcaaa aaccgttttg
    20641 gttcgaccaa tgaaataggt atctttgaaa tgcagtcggg tggtctagtt gaagttttga
    20701 atccaagtca agtttttctt gaagaacgtt tagacggtgc aacaggatct gctattgttg
    20761 ttactatgga aggaacaagg cctattttag cagaggttca agctttagtg actccaactg
    20821 tttttgaaaa tgctaagcgc acaaccacag gtcttgactt taatcgtgtc agtcttatta
    20881 tggcagttct agaaaaacgt tgtggtttat tgcttcaaaa tcaagatgcc tatttaaaat
    20941 cagcgggtgg tgttaaattg gatgaaccag caattgattt agctgtagct gtagctattg
    21001 cttccagtta caaagaaaaa cccacaaatc cacaagaatc atttattgga gaaattggac
    21061 tcactggtga gattcgtcgc gtgacacgta ttgagcaacg tattaatgag gctagtaaat
    21121 tgggctttac taaaatttac gcaccaaaga attctctagc tgggattgaa atacctaaag
    21181 gcattgatgt tattggtgtg acaactgtta gtcaggtctt aaaagcagtg ttttcaacat
    21241 aatgattaga ttcggttcag ccgaatcttt tttggtggcg agagacattt cgtgaaaaat
    21301 aatgggcaaa atgataatat aaaagggttg gtatttagta aatatcgaat gatgttacac
    21361 taacaaagga gatctctatg accacttatt ttgataactt tttaaaaacc aatcaagcct
    21421 atgctgactt acatggaaca gctcatctac ctatcaagcc aaagacaaag gttgctattg
    21481 taacctgtat ggattctcgt ttacacgttg ctcaagctct agggctagct ttaggagatg
    21541 ctcatatttt acgtaatgct ggtggacgag ttacagatga tgttctacgt tctttagtta
    21601 tttcgcaaca acaattagga acacgagaaa tcgttgttct acaccataca gattgtggag
    21661 cacagacgtt tactaacgaa gcttttgctg ctcaattaca gagagatttg ggtgttgata
    21721 tgcatggaca tgattttctt ccttttaatg atattgaaga aagcgttcgt gaagatgtgg
    21781 caaaattaca tgcttctcca ttaatcccaa atgatgttgt tatttctggt gctatttatg
    21841 atgtagatac tggtcgtatg gtggaagtta aatagaaaaa atcctgtcct tagcgatggg
    21901 tttttttatt ttagaaaact agatttaaag gtaacagtta ggcttatcat gccttatgct
    21961 ggaattcaga cagaggaaac atcttttgct atactgaaac tatattataa aagcggagga
    22021 aatttaaaat gaaaaaatat gatgtcattg ttcttggttt tggtaaagca ggtaaaacat
    22081 tagcagcaaa attagcaaca caaggtaaaa gcgttgcaat ggtcgaagaa gacaataaaa
    22141 tgtatggggg aacatgtatc aacattggat gtattccgac aaaaacgctt ttagtatcag
    22201 ctagcaaaaa tcacgatttt caagaagcta tgactactag aaatgaagtt acaagtcgtt
    22261 tacgtgctaa aaattttgct atgttggata ataaagacac tgtggatgtt tataatgcta
    22321 aagctcgctt tatatcaaat aaagtggtcg aattaacagg aggagctgac aaacaagaac
    22381 ttacagctga tgttattatt atcaatactg gtgctaagtc agtacaatta ccaattccag
    22441 gattagctga ttctcaacat gtttatgatt caacggcaat ccaagaattg gctcatcttc
    22501 ctaaacggtt aggtatcatt ggaggcggta atattgggct tgaatttgcg actttatatt
    22561 ccgaattagg gagcaaagtg acagttattg atagtcaatc tcgtatcttt gcgcgtgagg
    22621 aagaggaatt atcagagatg gctcaagatt atttagaaga gatggggata tcttttaaat
    22681 tatcagccga tatcaaatct gtgcaaaatg aagatgaaaa cgttgttatt agctttgagg
    22741 atgaaaaatt aagctttgat gcagtattat atgcaacagg acgtaagcca aatacagaag
    22801 gtttagcact tgaaaataca gatattaaat tgacagaacg tggagctatt gctgttgatg
    22861 aatattgcca aacttctgtt gagaatattt ttgcagttgg tgatgttaat ggcggccctc
    22921 aattcacata tatttcttta gatgactcac gtattgtctt aaattatttg aatggtgata
    22981 aggattattc tctaaaaaat cgaggagcag taccgacttc aacctttaca aatcctcctt
    23041 tagcaacggt aggtctagat gaaaagaccg ctaaggaaaa aggatatcaa gttaaatcta
    23101 atagccttct tgtttctgcc atgccacgtg cacatgttaa taatgacctt agaggtattt
    23161 ttaaagttgt ggtagatact gagacaaatc ttattttggg agctcgctta tttggtgcag
    23221 aatcccatga attaattaat atcatcacaa tggcaatgga taataaaatc ccatatactt
    23281 atttccaaaa acaaattttc actcacccaa caatggtaga gaattttaat gatttgttta
    23341 acttttaaga atatttttaa aaagtaaggc aattttgctt tacttttttt atcgaa
//
LOCUS       DATRSD010000027        23136 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRSD010000027 DATRSD010000000
VERSION     DATRSD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23136
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.027"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..158
                     /locus_tag="VBD85_000843"
     CDS             <1..158
                     /locus_tag="VBD85_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EbsA protein"
                     /protein_id="HEO7133407.1"
                     /translation="IFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYD
                     KKALTKS"
     gene            complement(145..342)
                     /locus_tag="VBD85_000844"
     CDS             complement(145..342)
                     /locus_tag="VBD85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO7133408.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEVP
                     NSDQDTVLAVKSCPTKALTIL"
     gene            388..912
                     /locus_tag="VBD85_000845"
     CDS             388..912
                     /locus_tag="VBD85_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7133409.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWLTAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            923..1606
                     /locus_tag="VBD85_000846"
     CDS             923..1606
                     /locus_tag="VBD85_000846"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO7133410.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFIGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    1627..1752
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1767..2297
                     /gene="infC"
                     /locus_tag="VBD85_000847"
     CDS             1767..2297
                     /gene="infC"
                     /locus_tag="VBD85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO7133411.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            2337..2537
                     /gene="rpmI"
                     /locus_tag="VBD85_000848"
     CDS             2337..2537
                     /gene="rpmI"
                     /locus_tag="VBD85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO7133412.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            2595..2954
                     /gene="rplT"
                     /locus_tag="VBD85_000849"
     CDS             2595..2954
                     /gene="rplT"
                     /locus_tag="VBD85_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO7133413.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(3195..5339)
                     /locus_tag="VBD85_000850"
     CDS             complement(3195..5339)
                     /locus_tag="VBD85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO7133414.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDDRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKAIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            5474..6631
                     /locus_tag="VBD85_000851"
     CDS             5474..6631
                     /locus_tag="VBD85_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO7133415.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQVFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILTPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            6631..7308
                     /locus_tag="VBD85_000852"
     CDS             6631..7308
                     /locus_tag="VBD85_000852"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO7133416.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            7402..8469
                     /locus_tag="VBD85_000853"
     CDS             7402..8469
                     /locus_tag="VBD85_000853"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO7133417.1"
                     /translation="MQVEVDLPNHSYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYAFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGTIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFDILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            8470..9636
                     /gene="aroC"
                     /locus_tag="VBD85_000854"
     CDS             8470..9636
                     /gene="aroC"
                     /locus_tag="VBD85_000854"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO7133418.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIIVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            9720..10055
                     /locus_tag="VBD85_000855"
     CDS             9720..10055
                     /locus_tag="VBD85_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO7133419.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LAAQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
     gene            10230..11579
                     /gene="gorA"
                     /locus_tag="VBD85_000856"
     CDS             10230..11579
                     /gene="gorA"
                     /locus_tag="VBD85_000856"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO7133420.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEVNNLNFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPDSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKGYIKADEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGEDNIKVYTSTFTSMYTAVTTNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(11622..12074)
                     /locus_tag="VBD85_000857"
     CDS             complement(11622..12074)
                     /locus_tag="VBD85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133421.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            12321..13466
                     /locus_tag="VBD85_000858"
     CDS             12321..13466
                     /locus_tag="VBD85_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7133422.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQETDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEDFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            13468..14682
                     /gene="thiI"
                     /locus_tag="VBD85_000859"
     CDS             13468..14682
                     /gene="thiI"
                     /locus_tag="VBD85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO7133423.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            14784..15962
                     /locus_tag="VBD85_000860"
     CDS             14784..15962
                     /locus_tag="VBD85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO7133424.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKHVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVMLYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKPNGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    16038..16117
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            16152..16466
                     /gene="rplU"
                     /locus_tag="VBD85_000861"
     CDS             16152..16466
                     /gene="rplU"
                     /locus_tag="VBD85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO7133425.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            16473..16811
                     /locus_tag="VBD85_000862"
     CDS             16473..16811
                     /locus_tag="VBD85_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO7133426.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            16833..17126
                     /gene="rpmA"
                     /locus_tag="VBD85_000863"
     CDS             16833..17126
                     /gene="rpmA"
                     /locus_tag="VBD85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO7133427.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            17342..18247
                     /locus_tag="VBD85_000864"
     CDS             17342..18247
                     /locus_tag="VBD85_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7133428.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            18256..18720
                     /locus_tag="VBD85_000865"
     CDS             18256..18720
                     /locus_tag="VBD85_000865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7133429.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVGVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            18704..19594
                     /locus_tag="VBD85_000866"
     CDS             18704..19594
                     /locus_tag="VBD85_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7133430.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLEKLRKN"
     gene            19770..20291
                     /gene="pyrR"
                     /locus_tag="VBD85_000867"
     CDS             19770..20291
                     /gene="pyrR"
                     /locus_tag="VBD85_000867"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO7133431.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            20340..21410
                     /locus_tag="VBD85_000868"
     CDS             20340..21410
                     /locus_tag="VBD85_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO7133432.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSILRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEILNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVSQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            21466..23064
                     /locus_tag="VBD85_000869"
     CDS             21466..23064
                     /locus_tag="VBD85_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO7133433.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRDRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLIMRDSLNNCLYVSDLEYIEFNKTTGKSIAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSHQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVAAVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFLFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLCD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKEAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYIFSGCQKALIMVDKGYS
                     LGKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKIKNFSISCQSI"
BASE COUNT         7610 a         3518 c         4657 g         7351 t
ORIGIN      
        1 gtatttttta catgcgaaaa tggcctaaaa aatatttttt agatgcacta gctattgaac
       61 ctaaatttaa gggagaagtg gagcttctcg ataatttgat taaaatggat tattttgaat
      121 gttatcgcta cgataaaaaa gctcttacaa aatcgtaaga gctttggtgg gacatgattt
      181 aactgctaaa acagtatctt gatcagaatt tggaacctct ttttctaggt tatctgtatc
      241 ggaaaattta acgataccat catcttgata atcaaaaata tttgaatatg tttggcaaag
      301 cccgcaggca atacattttt ctggaattag tgcaactttc atagtattat tgtaatactt
      361 attgatacga ttaacaaggg ggaagtcatg gcgaaaaaac catgggaaaa aaaagttgtg
      421 gaaaacaata gtcatagaaa agacaaaatt acaagaacga gtagaggtgt ggtgtcaagt
      481 actccttggc ttaccgcttt cttgagtgca ttctttgtta ttgtcgttgc tattttattt
      541 atagtatttt atacttcaaa tagaggagaa gacagagcaa aagaaacttc tggtttttat
      601 ggtgcttcaa gtcaaaaagt taatagttct aaaacgaaaa aagcatctac aagtaagaaa
      661 acaacgtcat catcaaaaac taaaactagt gataaatcaa caacatcttc tagtacagta
      721 gcaagttcga gtgagacatc gacatcatct tcagtaaacg gtggaacaat cgtagttcaa
      781 ccaggtgagg gagcagcctc catagctgct cgtgcaggta tctcagttgc tcgtttacaa
      841 gaacttaatc ctggtcatat gacctcaggg tattggtatg ctaatccagg cgatcctgtg
      901 gttgtaaatt aaggggagga ttatgaactc aattaatatt gctattgatg gaccagcatc
      961 aagtgggaaa agtactgtcg ctaagattat tgctaaaaat ttgaattaca cttaccttga
     1021 tacaggtgct atgtatcgtt gtgctactta tctggcttta cagcatggat atgaagcaca
     1081 agatgtttcg aaaattttag gactcctagc tgaaagaccg atttcttttg gtaaagcaga
     1141 agatggcagc cagacagtct ttattggcac agaagaagtt actttagcta tccgtcaaaa
     1201 cgatgttact aacaatgtct cgtgggtttc tgctatccca gagattcgag aagagttagt
     1261 aaaccagcag cgtcgtatag ccaaagatgg tgctattata atggatggtc gtgacatagg
     1321 aacagttgtt ttgccagatg cagaattaaa gattttttta gttgcttctg ttgatgaaag
     1381 agctgagcgt cgctttaaag aaaatcaaga aaaaggcatt gagagtgatt ttgaaacact
     1441 gaaatcagaa attgctgctc gtgattataa agatagccat cgagaagttt caccattaaa
     1501 ggcagcagaa gatgctattg aatttgatac tacaggagta tcaattgaag gtgttgtaac
     1561 ttttattcaa gaaaaagcag aaaaaataat tgacatgaag aattagtcat gatatactag
     1621 ttgcaatgag aaaagcagaa gtgagaactt ctcgccttgc gacttaggtt gtctggccca
     1681 acatgtcaag attccatcta ggaagattat atgtgcgggc ttgatttatc aagtccgctt
     1741 tcgtttttct ctaaaaaaat aaagaggtga agatcatagc taaaaaagat ctattcatta
     1801 acgatgaaat tcgcgttcgt gaagttcgtc tggttggtct cgagggtgaa caattaggta
     1861 ttaaaccatt gtctgaagct caagcaattg cagatgatgc taatgttgat ttggttttaa
     1921 ttcagccaca agctacacca cctgttgcga aaattatgga ctatggtaag ttcaaatttg
     1981 agtatcaaaa gaaacaaaaa gaacaacgta aaaaacaaag cgttgttact gttaaggaag
     2041 ttcgtcttag cccagttatt gataaagggg attttgaaac aaaacttcgt aacggtcgta
     2101 agttccttga aaaaggtaac aaagttaaag tttctatccg attcaaagga cgtatgatta
     2161 ctcataaaga aattggagca aaggtattgg cggaatttgc tgaagcgaca caagatatcg
     2221 ctattattga gcaaagagct aagatggatg gtcgccaaat gtttatgcag cttgcaccaa
     2281 ttccagacaa gaaataattg tcgactttat agtattattt aagaggagaa tttaaaatgc
     2341 caaaacaaaa aacacaccgc gcatcagcta aacgctttaa acgcacaggt tcaggtggat
     2401 tgaaacgctt ccgcgcgttt acttcacacc gtttccacgg taaaactaaa aaacaacgtc
     2461 gtcatcttcg taaagctact atggtttcat caggagattt caaacgtatt aaagcaatgc
     2521 ttactcgcct taaataataa ggtctctgta aacatttact acatattata aaggattatt
     2581 tggaggaaat ataaatggct cgtgttaaag gtggagttgt ttcacgtaaa cgtcgtaaac
     2641 gcgtacttaa attagctaaa ggttactatg gtgcaaaaca tatcttgttc cgtactgcaa
     2701 aagaacaagt aatgaattct tactactatg cataccgtga ccgtcgtcag aaaaaacgtg
     2761 acttccgtaa attatggatc actcgtatca atgcggctgc tcgtatgaat ggtttatcat
     2821 attcgcaatt gatgcacggt ttgaaattag ctgaaatcga agttaaccgt aaaatgcttg
     2881 ctgatttagc agttaacgat gcagcagctt tcacagctct tgcagatgca gctaaagcta
     2941 aacttggtaa ataagaatta aaaaagttgg gtctacctaa cttttttaat tactagaaag
     3001 aaagtaccta tattataatt taagtgaaaa gtttcgttct atatatgtag aatgaaactt
     3061 ttttgtttta attcgatatc tttattattg ttataatttt cctttttgat ttttattgtt
     3121 ttatagtatt attccgtata aaaagcacct caatcgttgg attgaggtgt ggattaaact
     3181 ttaaatggca aggattattt tttagatgtt ttatgtgtct tattaggatc ttgcaattcc
     3241 aagtaacttg gagcgtggaa gagatcaact gttgatttgt tgttatgttt gctgtatatg
     3301 cttgttgaac gatctttcaa tttacgttct ttagctttga gtgctttaag agaatgtgtg
     3361 taattgaatt tttcaacttc tacagttttc aaaccattgt tttcatcaaa tcgtagtaaa
     3421 tctccagttt gaatcgaatc acttgtagat aattgtttat tagtagcatc tcgaatagct
     3481 tttatttcag ctttagttgt ttcatcagga tttgtgattt cttgtcctga atcagtgtaa
     3541 tagagatgtc cactatagtt agtgtattta ggagtaatat actgaccagt tgttctcata
     3601 gcgaccattt gtttattatc tttagataat aagtcttgtc ctaactgagt atatttagac
     3661 gtgtctatcc ctagaatatg aagtaaagtt ggtaaattat ctacctcacc accgtatgta
     3721 ttactgataa aaccttttga atagccagga atgtggatca tatatggaac acgttgtaac
     3781 atagcgttgt cgtattcaga ccatgtttca gggtctttac ctaataattc agctaagcta
     3841 ggattgcgtg tattagaaat accgtagtga tccccataca ttacaataat agaattatca
     3901 tacacaccag cagcttttag atattcaaaa aatgatttaa gagctgtatc aaggtagttt
     3961 gctgtggcga aataaccgtt aatcgtttca tcgttagtct ttgcaagtgg gaatccctct
     4021 tcgtcagact ctcccttcaa acttgtgtaa gggtaatggt tacttactgt aataaattta
     4081 gtataaaacg gttgttgcat gtgctctaga tatttgatag agtcagcaaa catatattta
     4141 tcattcaaac cgtattggaa tgaattatct ttggtttgtt ttgagaagta gctagagtca
     4201 aagaagtagt cataacccca ctgtttgtaa gcattatttc tattccaaaa tgttccgacg
     4261 tttccgtgga agaccgcact actgtaacct ccattttgag ctaagatatg tggagcagca
     4321 aattgtgtat tttcaccacc ataattaacc atgaatgaac cactacttag accaaaaagt
     4381 gagttttcca tgagtgtctc agcatcggat gtcttaccag ctttgacttg atggaagaag
     4441 ttagagaagg aaatagtctc tttcgaatga taaaggctgt taataaatgg agtaaccaca
     4501 tgttctttac catcaacatt taatttatag tcaatcaaga attgttgaaa actctccaag
     4561 tgaatcatga taacgtttcg cccttttccg ataccataat attctggatt gggtttggca
     4621 taatgctctg ctacatattt tttggcagtc gccaattctt gtgcagtggc accatttctt
     4681 tctttctgtg cttgataagt ttgattacca ctataaatcg aaaatgatgg caagccaaga
     4741 gctttaacaa tataggtgtt tgaaaaacca cgacttagta gttctggacg atcaatttca
     4801 gctaagaata agttaattga aaagagtaaa ccagacaaag ctgtgataga aaaactagca
     4861 cgtttattaa atggacgatc atctaagtgg attttcttgg ttgcaaacaa gaaaataagg
     4921 ataatgaaat cgaaaacgta gacaagatcc caaattctca atagattaag agcagagtct
     4981 ccaaggccag ccgaagtttt actactagcc aagacggcgc taactgtaat gaaatcggaa
     5041 aactctctat agtagattgc gtttgcaatt agaaggatat ttacgatagc ataggtgata
     5101 aaagcggtaa tataaaaagc tttggttcgc ttaacatata aggccaaacc tatgattaag
     5161 agtcctaagg ggatggggtt tataatggtc aaaaacaact gataggaatt ttctaaacct
     5221 aaattaaaat cagtatggta agcccaaatt gtctttaacc aatatataac tactaacaat
     5281 aaaataaagc cgagtcgtgt attgatcgca cggctagcaa aatctttaat ttttttcaca
     5341 gtaggaactt cctttaattt ttttcctaaa gataaaatct cagattaaat tataacataa
     5401 aattttgtaa catacggtaa agaaaggtcg aattcagtat gaaaaaagat aaaaatttgt
     5461 tataatactg tttatgaata aacttaaagt gaattctgtc gttgaaagaa aaatcaaatc
     5521 aggtgctcag ttactggaaa aaaaagattt tgataccagt ttagttaacc agttggttca
     5581 acttttttca cagtcaaatc aattcttagg gatggcctat ctttcaccac aaaataaagg
     5641 tattggttgg ttactatcaa gacaagtttt tgattttaac catgattact ttgtatcgct
     5701 attcgaaaaa tctagagaga agcgtcaaaa atttgaaaaa tctagccaaa caacagccta
     5761 ccgcttgttt aatcaggatg gagataactt tggtggccta actatagatt tttatagtga
     5821 ctatgctctt ttttcgtggt acaatgaatt tgtttatact aatcgacaaa tgattgttgc
     5881 agcctttaag caggtctatc ctaatattaa aggggcatat gaaaaaattc gtttcaaagg
     5941 tttagacttt gaaagtgctc atttgtacgg tcaagaggct cctgaatcat ttttgatttt
     6001 agaaaataat atcaaatata gtgtcttttt gaatgatgga ttaatgacag gtattttcct
     6061 tgaccaacat gatgtcagaa aagccttagc aactaatcta tcagaaggta aaaaagttct
     6121 aaatatgttt tcatacactg cggctttttc tgtagctgca gctgttggag gggcattaga
     6181 gacaacttct gttgatttag caaaacgctc gcgtgaactt tcaaaagcac actttgatgc
     6241 taatcagatt gtcacagata accatcgatt tatcgttatg gatgtttttg aatattataa
     6301 gtatgccaaa agaaaacatc tatcatatga tgtgattgtt attgatccac caagttttgc
     6361 tcgaaataaa aaacaaactt tttcggttac taaagattac tataaattaa ttgaacaagc
     6421 tttagatatt ttaacccctg gtggaactat cattgcatca actaacgcag ctaatctaac
     6481 cgtatcacaa tttaagaaac aattggaaaa ggggtttggt aaagcttcac ataattacat
     6541 tagcttacag cagttgcctg aagatttcac tgtaaatgat aaggaccaac aaagtaatta
     6601 tttaaaagta tttacaataa aggtaaaata atgaaaatag tagtaccagt aatgcctcgc
     6661 agtcttgaag aggctcaaga aatagattta tcaaaatttg atagtgttga tattattgaa
     6721 tggcgagctg atgccttacc aaaggatgac attattaatg tagctccagc tatttttgag
     6781 aaattcgcag gtcatgaaat tatttttact cttcgtacaa cgcgtgaagg tggtaatatt
     6841 gtcttatctg atgctgagta tgttgagtta atccagaaaa ttaattctat ctacaatcca
     6901 gattatattg attttgagta tttttcacat aaagaagttt ttcaagaaat gctagaattt
     6961 ccaaatttag tcctgtctta tcacaatttt caagagacac cggagaatat tatggagata
     7021 ttttcagaat taacagccct agcaccacga gttgtgaaaa tcgcagtaat gccaaagaat
     7081 gaacaagatg tcttagacgt tatgaattac actcgcggtt tcaagactat taatcctgat
     7141 caagtttatg cgacggtatc tatgagtaaa attggacgta tttctcgttt tgctggtgat
     7201 gtaactggat ctagttggac atttgcatat ttagattcat ctatcgcacc cggacaaatt
     7261 actatttcag agatgaagcg tgtcaaagca ttgcttgacg ctgactgata cgagataaca
     7321 aaatgttaaa tatactgatg agatccctag caattgctta gggtttttgt cttattaaaa
     7381 caaacatgag aggacattag aatgcaggtt gaagttgact taccgaatca ctcttatcat
     7441 atcaaaattg aagaaggatg tttttctgag gctggtgact gggtatcaca tttatggcag
     7501 aaacaaatga taactattat tacagatagt aatgtagaga tattatacgg ggagtcgctt
     7561 gtcaaccagt taaaaaaaca aggttttaca gtacatgttt ttagttttgc tgcgggtgaa
     7621 gctagtaaaa ctttggaagt tgctaatcgt atttatgctt ttttagcaaa gcatcatatg
     7681 accagaagcg atgggatcat tgcacttggt ggaggcgtcg tcggagattt agcagcgttt
     7741 gttgcttcta cttatatgcg gggaatccat tttcttcaaa ttccaactag tttaacagca
     7801 caggttgatt cttccattgg cggaaaaaca ggagtcaaca cttcattcgc taaaaatatg
     7861 gtaggtactt ttgcacaacc tgatggtgta ctgatagatc ctgttactct aaaaacattg
     7921 ggcaatcgtg aattagttga aggtatggga gaagtcatca aatatggctt gattgatgat
     7981 ataaaactct ggcatatttt agaagagatg gatgggacta ttgatagtat ccttgataat
     8041 gcattagcta ttatttatca ctcttgtcaa gttaaacgaa agcatgtttt agctgatcaa
     8101 tatgataaag ggttaaggat gcatttgaat tttggccaca ctatcggtca tgctatcgaa
     8161 gtacatgctg gctatggaga aataatgcat ggagaagctg ttgcaattgg aatgatccag
     8221 ctttctcgtg tggctgagcg taaaaatctt atgccaagag gtatcagtca agatatttat
     8281 aatatgtgtt taaaatttgg ccttccggtc cactatgcgg aatgggataa ggacgtctta
     8341 tttgacatat tgagtcatga taaaaaagca agtggacaat ttattaaaat tgttatactg
     8401 cctcaattag gtagtgcaac agtccaccaa atacctttag aagaaatgag agattatttg
     8461 gagaaataga tgagatattt aactgctggt gagtcacatg gaccgagttt aacagctatt
     8521 atcgaaggga ttcctgcggg actgaaatta tctgctaaag atattaatga agatttaaaa
     8581 cgccgccaag gtggttatgg tcgtgggaac cgaatgaaaa ttgaaactga tcaagtgatc
     8641 attagttctg gtgtgcgcca tggaaaaacc ttgggttcac ctattacact gacagtaact
     8701 aataaggatc atagcaaatg gctggacatc atgtcagtcg aagacattga agagcgtcta
     8761 aagcaaaaac gtcgtattaa gcatcctaga cctgggcacg cagatttagt aggtggaatc
     8821 aaataccgat ttgatgattt gagaaatgct ttagagcgtt cttcggctcg tgagaccaca
     8881 atgcgagtag ctattggtgc gattgctaaa cgtattttga aagaaattgg tatcgaaatt
     8941 gccaaccata ttgtagtgtt cggtggcaag gagattactg tccctgataa gctaacagtt
     9001 caacaaatca aagtgttatc tagtcaatcg caagtagcta tcgtcaatcc atcatttgaa
     9061 caagaaatta aggattatat tgacagtgtg aaaaaggctg gagatactat cggaggagtg
     9121 attgaaacta tcgttggagg agttcccgtt ggtttaggct cttatgtaca ctgggataga
     9181 aagttagatg ctaagatagc gcaagcagtt gtatctatca atgcttttaa aggtgttgaa
     9241 tttggcctag gttttaagtc agggttttta aaaggaagtc aagtaatgga ctccatatca
     9301 tggacaaagg accaaggtta catacgtcag tcaaataatt taggcggttt tgaaggcggt
     9361 atgactaatg gagagcctat tatcgttagg ggagtaatga aaccaattcc tacgctttac
     9421 aagccactaa tgtcagttga tattgatact cacgagcctt atagagctac agtggaacgt
     9481 tctgatccca cggcacttcc ggctgcaggt gtcgttatgg aagctgtagt agccaccgtc
     9541 ctggtgacag aagtgctaga aaaattttct tcagacaaca tgtatgaact aaaagaagct
     9601 gtaaaactat atcgcaatta tgttaatcat ttttaaatga aaactagtag tattcaattg
     9661 caaaaaaagg tataaaagtc taaaatagtg gtaagttcaa gtagaaaaag gagtaactta
     9721 tggctaatgt atatgattta gcaaatgaat tagaacgtgc tgttcgtgct ttaccagaat
     9781 accaagcagt tttaactgca aaagcagcta ttgaaaatga tgcagatgca caagtgcttt
     9841 ggcaagactt tttggctgcc caatcaaaag ttcaagaaat gatgcaatct ggccaaatgc
     9901 caagtcaaga agaacaagat gaaatgtcta aacttgggga aaaaattgaa tccaatgacc
     9961 ttttaaaagt ttattttgac caacaacaac ggttgtctgt ctatatgtct gatatcgaaa
    10021 aaattgtctt tgcacccatg caggacttga tgtaatatta taaaagagag agctccttga
    10081 gttttctctt ttataatatt tcaatttatg atagtctact gaactagtat tgcccacatt
    10141 atattggttt tgcattctac gtagcagaaa attgtgactg taaacacttt gtgatagagt
    10201 ataggtatca atgattcaag gagagagtaa tgtcaaaaca atatgattat atcgttattg
    10261 gtggaggtag tgcaggcagt ggtaccgcta atagggcagc catgtatgga gcaaaagtcc
    10321 tgttaattga aggtggacaa gtaggtggaa cttgtgttaa cttaggttgt gtaccgaaga
    10381 aaatcatgtg gtatggtgca caagtttctg agacactcca taagtatagt tcaggttatg
    10441 gttttgaagt caataatctt aattttgatt ttactactct aaaagctaat cgcgatgctt
    10501 acgtgcagcg gtctagacag tcgtatgccg ctaattttga gcgtaatgga gtcgaaaaga
    10561 ttgatggatt tgctcgtttt attgataacc atactattga agtgaatggt cagcaatata
    10621 aagctcctca cattactatt gcaacaggtg gacaccctct ttaccctgat attattggaa
    10681 gtgaacttgg tgagacttct gatgattttt ttggatggga gaccttacca gattctatat
    10741 tgattgttgg ggcgggctat atcgcggcgg aacttgctgg agtggttaat gaattaggcg
    10801 ttgaaaccca tcttgcattt agaaaagacc atattctacg cggatttgat gacatggtaa
    10861 caagtgaggt tatggctgaa atggagaaat caggtatctc tttacatgct aaccatgtac
    10921 ctaaatctct taaacgcgat gaaggtggca agttgatttt tgaagctgaa aatgggaaaa
    10981 cgcttgtcgt tgatcgtgta atatgggcta tcggccgtgg accaaatgta gacatgggac
    11041 ttgaaaatac cgatattgtt ttaaatgata aaggttatat caaagcagat gaatttgaga
    11101 atacttctgt agatggcgtg tatgctattg gagatgttaa tgggaaaatt gccttgacac
    11161 cggtagcaat tgcagcaggt cgtcgcttat cagaaagact ttttaatcat aaagataacg
    11221 aaaaattaga ttaccataat gtaccttcag ttatttttac tcaccctgta attgggacgg
    11281 taggactttc agaagcagca gctatcgagc aatttggaga agataatatc aaagtctata
    11341 catcaacttt tacctctatg tatacggctg ttaccactaa tcgccaagca gttaagatga
    11401 agctcataac cctaggaaaa gaggaaaaag ttattgggct tcatggtgtt ggttatggta
    11461 ttgatgaaat gattcaaggt ttttcagttg ctatcaaaat gggggctact aaagcagact
    11521 ttgatgatac tgttgctatt cacccaactg gatctgagga atttgttaca atgcgctaac
    11581 gctatcaaag aatacaaaaa aagctaggga acctagcttt tttaaggtgt tgttgtattt
    11641 tcaatagttg aggtatcggt agtagtatta tttgtttgat cattgttagt actagatgat
    11701 gtttgatcgt tattggcagc agaatttgaa ctgctactcg gagtactact agcagaggaa
    11761 ttttctagtg tttggctgga tgatgccctt gtctcatctt tttcagtaga aacttggtta
    11821 ctagaatttt gtccactttc tcttataatg acggtttttg aagtagaaga atcagtctta
    11881 ctagagtctt tgtgattatt aatcgcatta gtaatagtaa tagaggcaac aataatactt
    11941 aaaacactac cgactaatgc aattgttttt tgaagtgcag aaaaacctgt ttttatatgc
    12001 ttagcgggtg attgagttga atcagtatta gtcttagtag actttttctg ttgtcttgaa
    12061 tatccttttg acatgacgcc ctccttaaat gtacatcaac tattatatga ttttatccta
    12121 aaaaaagcta aagaaaaaat aatttaaaac atttaaataa gcaacctagg ttattaagct
    12181 atgttagtgt aataggtagt gagttttgtg ataaattatg agaattatca taaatattgt
    12241 tttgggatgt tcacaaacta atataaatag taggaaaatt atgctataat aacactgatt
    12301 gactttagaa ggacagattt atgatttatt ttgataattc ggcaactact attccctatc
    12361 cagaagtttt aaggacttat caagaagtag ctagtaaaat ctatggaaac ccctcaagtt
    12421 tacatgagct aggaacaaca tcttctcgga ttttagaggc tagccgtaaa caaattgcta
    12481 gtttacttga gcttaaagca aatgaaattt tctttacctc tggtggtaca gaggccgata
    12541 attgggttat taagggtctt gcgtttgaga aacaacattt tggaaatcat attattgttt
    12601 ctgatatcga acacccagct gttaaagagt ctgctaaatg gcttggagag tatgggtttg
    12661 agattgatta tgcgccagta gatgataaag gatttgtaga cgtagaagca ctggtcaaat
    12721 taattaaacc agaaacgatt cttatttcaa ttatggcaat aaacaatgaa attggttcta
    12781 ttcagccaat aaaagctatt tcagacttgt tgtcagataa acctactata tcgtttcatg
    12841 ttgatgctgt tcaagcaata ggtaagatac cgactaaaga ttatttgact gagcgagttg
    12901 attttgcatc tttttctagt cataaatttc acggcgttcg tggggttggc tttctatata
    12961 ttaaagaagg aaaacgtatt tcaccattac taacaggtgg tggacaagaa actgatttac
    13021 gttcaactac tgagaatgtc gcagggattg cagctactgc taaagcttta cgaatggtta
    13081 tggacaagga agtagtagct attcctaaaa taagtaaaat gaaaaccatt attcatgatg
    13141 aattagccaa atatgaggat attacgttat tttcgggtaa ggaagatttt tcacctaaca
    13201 ttatcacgtt tggtatcaag ggtgtccgag gagaagttct tgtccatgcc tttgagggac
    13261 atgatatttt tatctcgaca actagcgctt gctcttctaa agcaggtaaa ccagcaggaa
    13321 ctcttatagc aatgggaatt tcaactaagt tagcccaaac tgctgtacgt atcagcttag
    13381 atgatgataa tgatatggga caagtagagc aatttttaac tatttttaaa caagtttatg
    13441 aaaaaactaa gaaagttaga ggataagatg cagtattcag aaattatgat tcgttatgga
    13501 gaactctcta ctaagaagaa aaaccgtatg cgcttcatca ataagttaaa aaataatatg
    13561 gagcatgtac tctccattta tccagatgtt tcagtaaaaa cagatcgtga tagaggacat
    13621 gtatatctca atggtacaga ttatcatgaa gttgcagagt ccttaaaaga gatttttggt
    13681 atccaagctt tttctccatc ttttaaagta gaaaaaaatg ttgatacatt ggtaaaagct
    13741 gtccaggaaa ttatgacttc cgtttataaa gatgggatga cttttaaaat taccgcaaaa
    13801 cgtagtgacc actcatttga attggatagc cgtgctctaa atcatacttt aggagatgct
    13861 gttttttcag tcttgccaaa tattaaggct cagatgaagc aaccagatat caatcttaaa
    13921 gtcgagatac gagatgaggc tgcttatatt tcatatgagg atattagggg tgcaggagga
    13981 ttaccagtag gaacatctgg aaaagggatg ctgatgttgt ctggtgggat tgattctccg
    14041 gtagcaggtt acctagcgtt aaaacgtggt gtagatatag aagcagtcca ttttgcaagt
    14101 cctccttata ctagcccagg tgcattgaaa aaagcacatg atttaacacg taaattgaca
    14161 aaatttggtg gtaatattca atttattgaa gttccattca cagaaattca agaggaaatt
    14221 aaggcaaaag ctcccgaagc ctacttgatg acgttaacac gtaggtttat gatgcgtatt
    14281 acagatcgta ttcgtgagga ccgaaatggt cttgttatta ttaacggtga aagtttagga
    14341 caggtggcaa gccaaacgtt agaaagtatg caagctatta atgctgtcac tgcaacaccg
    14401 attattcgtc ctgtggtcac gatggataag ctagaaatta ttgatattgc tcaaaaaata
    14461 gatacttttg atatttcaat tcaaccattt gaggattgct gtacgatttt tgcaccagat
    14521 cgcccaaaaa ctaaccctaa aattaagaat acagaacagt atgagaaacg tatggatgta
    14581 gaaggtcttg tagagagggc agttgcaggg attatggtaa ctactattca acctcaagca
    14641 gatagtgatg atgttgatga cttgattgac gatttattat aatgaatttt taaactacag
    14701 atttactctg tagtttttta ttaaaatgat tgcgctatca taaggttata aaaaatgcta
    14761 taatacgaat aaaggaggca agtatggcaa gaatccctta tcagaaaaca cttatttttt
    14821 gtgtagccgt tattattgct atttttattt tggggttatc taaggattta gcacaatcta
    14881 aagggcaaaa ggttgcaaat aataatactg taaaaacagc acgtgtggtt gcaaacggag
    14941 atattttgct gcacgatgta ctatatgcca gtgcgcgcca acccgacggc acttataatt
    15001 ttactcctta ttttaaagag gtaaaatctt ggattgagag cgctgattta gcgattggtg
    15061 attatgaagg gacgattagt tcagaatatc ctttagcggg ctatccttta tttaatgctc
    15121 ctaatgagat tgcaactact atgaaagaaa ctggatatga tgtcgttgac ttagcacata
    15181 atcatattct ggattcacaa ctggcaggtg ctatcaatac tgtcaagact tttaatcgtc
    15241 tgggattgga tactattggg gtttatttaa aggatagaaa taaagaagat atcttaatta
    15301 agcatgtaaa tggtattaaa attgctattt taggttattc gtatggctac aatggtatgg
    15361 aagctaatgt ttcaaagagt gactatgaaa aacatatgtc tgatttagat actaaaaaga
    15421 ttaagcaaga tattaaaaag gctgaaaaag aagcagatat caccattgtc atgccacaga
    15481 tggggataga atatcaaaaa aaacctacta cagaacaagt aatgctttat catagtatga
    15541 ttaaatgggg ggcagatatt atattcggag gacaccctca cgttgttgaa ccgtctgaag
    15601 tgataaaaaa agatggccag aaaaaattta ttatttactc tatgggaaat tttatttcaa
    15661 atcagcgttt agaaacggtt gatgatatct ggacagaaag aggattatta atggatgtaa
    15721 ccatagagaa aaaaggtcaa aaaacagtaa ttaaaaaagt taaggcgcat ccaacactag
    15781 tagaagcaaa accaaatggc cgttatgttg aagaaggctt tccagcttat gattatagga
    15841 cgctagtttt agaggattat attgaaggtg gtaagaaacg ttctacgatt gatcagacta
    15901 tgaaagataa agttgataaa gcatataagg agatgaatga gttagttaat ttgaagtggt
    15961 aaagttgata attttatttt gttttatctt gcataaaaaa agagtttatg ataaaatttg
    16021 aatgttgact aaatgcacat cctgtgcaac cgcgcgaaaa tcgttagatt aagtagagca
    16081 aggctctcac gatttatagg cgagtcttca cagagggaaa cctcacaaga aatttttata
    16141 ggaggtgcat catgagcaca tacgcaatca tcaaaactgg tggtaaacaa gttaaagttg
    16201 aagtaggaca agctatctac gttgaaaaac ttgacgttga agcaggagca gaagttacat
    16261 ttaacgaagt tgttcttgtt ggtggtgaaa caactaaagt tggtactcca gttgttgaag
    16321 gagctactgt tgttggtact gttgaaaaac aaggcaaaca aaagaaagtt gtttcttaca
    16381 aatacaaacc taaaaaaggt agccaccgta aacaaggtca ccgtcaacct tatacaaaag
    16441 ttgttatcaa cgcaatcaac gcttaatttt tcatgattaa agcaactttt actcgtaatc
    16501 aatcaggcta tctttatagt gctgaaatca gcggtcatgc tggtagtggc gagtacggct
    16561 ttgatgtgat ttgtgcagct gttagtactt tatcaattaa ttttattaat tcattagagg
    16621 ctttaacaac ctgccaagcg caattgatta tcaatgacgt tgaaggtggt tatatgaaga
    16681 tagatttatc ttctatacca caacataaag aggataaggt tcaattatta tttgagtctt
    16741 accttcttgg aatgactaat ctgtctaaag attcttctga atttgttagt acagtagtca
    16801 tgacacacta atttaagaga ggacacaaca ttatgttaaa aatgaatctt gctaacttgc
    16861 aacttttcgc ccacaaaaaa ggtggaggtt ctacatcaaa cggacgtgat tcacaagcaa
    16921 aacgtcttgg agctaaagca gctgacggac aaacagtatc aggtggatca attctttacc
    16981 gtcaacgcgg tacacacatc tatccaggtg ctaacgttgg acgtggtggg gacgatacac
    17041 tttttgctaa agttgaaggt gttgttcgtt tcgaacgtaa aggacgcgat aaaaaacaag
    17101 tgtctgttta cccaatcgca aaataatgcc tagttaagac tttatgacta ttcataaggt
    17161 cttttattat tgtcagagct ttcattttgt aacaaaagac aatatgttaa gagatttatc
    17221 attaatactc ataaactttg ttgttctttt atagtgaaat agtcctaatt catgaagaat
    17281 tttccgcaag gatagttatt ttttggtata atgaagggta ctaaaatgag gaagtgataa
    17341 aatgaatatt caacaactgc gttatgttgt tgctatcgca aatagtggta cttttcgtga
    17401 agcagctgca aaactatttg ttagtcagcc aagtttatct gttgccgttc gtgatttgga
    17461 aacagaatta gggtttcaga tttttacgcg tacaactact ggtgcagtgt tgaccaatca
    17521 aggcatgact ttttatgaga atgctttaga agttgtgaaa agctttgatt cattcgaaaa
    17581 acagttttcg caatctgaag ctacagagca agaattctca atagcaagtc aacattatga
    17641 ttttctacct cctttaatca cagcgttttc aaaatgtaat gataatttta gctatttccg
    17701 tattttcgaa tcaacaacaa tacgtatctt agatgaagtt gctcaaggga atagcgaaat
    17761 aggtatcatt tatatcaata gtcaaaataa aaaaggtcta cttcagcggt tggacaaatt
    17821 ggggttagag tttgtagagc ttattccttt taaaactcat atttatcttg ggaaagacca
    17881 tcccttggct agtaaaacat ctctgataat gacagattta gaaggattac caaccgtacg
    17941 ttttacacag gatagagatg attatcggta ttactcggag aattttgtgg aagtacttga
    18001 tagttctgtt acctataatg ttactgatag agcaacatta aatggtattt tagaaagaac
    18061 ccaagcttat gcgacgggct caggattctt ggatagccgc agtgtgaatg gtatcactgt
    18121 tattccatta gaagatcatc tagataacca aatgatttat atcaaacgaa aagatcgtaa
    18181 tttatctcag atggctttaa aatttgttgc tgttatggaa gagtatttta agggatttaa
    18241 ttcataggag aagtaatgag aaagattatt ataccaataa taactatttt gttgattgct
    18301 ttagatcaac tatctaagtt atggattgtt aaacatattg aactaaacca aattaaggaa
    18361 ttcattccaa atatcgttag tctaacttat ctgagaaatt atggtgctgc attttctatt
    18421 ttgcaaaatc aacaatggct ttttacctta attacaatct ttgttgttgg tgttgctatt
    18481 atttatttga tgaaacatat taatggaagt tattggttat taatttctct aaccttaatc
    18541 atctctggtg gattaggaaa ctttatagat cgcttaagat taggttatgt cgttgatatg
    18601 gtgcatttag attttattaa ttttgctatc tttaatgtag cagactcata tctgacaatc
    18661 ggtattattt gcttaatgat tgcactttgg aaagaggaaa gtaatggaaa tcacaattaa
    18721 aatagcagga gttaggctag ataaagcttt agcggataat tctgaactgt cgcgtagtca
    18781 agctaatgaa gaaattaaaa aagggattgt gttagtaaat ggacaagtaa aaaaagcgaa
    18841 atatactgtt caagaaggag atcggattac ttttgatatt cctaaagagg aagtactaga
    18901 ttatcaagct gaaaatatcc ctttagatat tatctatcaa gatgatgacg ttgctgtggt
    18961 taataagccg caagggatgg ttgttcaccc ttctgcaggc cacagttcgg gcaccttagt
    19021 taacgccttg atgtatcata ttaaggattt atcatcaatt aatggcgttg ttcgcccagg
    19081 gatcgttcat cgtattgata aagatacttc tggtttattg atggttgcaa aaaatgatag
    19141 agctcaccaa gtgttggctg aagagttaaa agataaaaaa tcgcttcgta aatatttagc
    19201 aattgtgcat ggtaatttac caaatgatcg aggtgtgatt gaggctccga ttggtagaag
    19261 cgataaagat cgtaagaagc aagccgtgac tgctaaaggt aagccagcaa taactcgttt
    19321 tcatgtttta gaacgttttg gtgattatac cttagttgaa ttgtcattgg agacaggtcg
    19381 aacacaccaa attcgggttc atatggctta tattggccat cctctagccg gtgatcctgt
    19441 ctacggtccc cgtaagactt tagggggtaa aggacagttt ttgcatgcac aaacactagg
    19501 ttttacgcac cctagtaacg gagagaattt gattttttca gtagaggttc cggaaatttt
    19561 tcagacaacc ttagaaaagt tgagaaaaaa ttaagttttt cttgaaaaat acttttacca
    19621 tttgctataa tataagcaaa tcattccttt aaccttatcc agtgagatag ttaaggagaa
    19681 acttcagact aaatcaatgc ctgagcggct ttgtcattct agagagtctc cttttgaagg
    19741 ggctctttta tcttagagag gatactgcaa tgaaaagaaa agaaattatt gatgatgtga
    19801 ccatgaaacg tgctattaca cgtattacct acgaaattat agaacgtaac aaaaatttag
    19861 ataatattgt cttagcaggc attaaaacaa gaggtgtttt tttagccaaa cgcattcaag
    19921 aacgcttgaa gcaattagaa aatttagata ttcccgttgg agaattggat actaaacctt
    19981 tccgtgatga tatgaaagtt gaagttgaca ccacaactat gccagttgac atcactgata
    20041 aagatattat attaattgat gatgttctct atacaggtcg cactattcga gctgctattg
    20101 ataatcttgt tagtttaggg cgtccgagtc gtgtcagttt agctgtatta attgaccgtg
    20161 ggcatcgtga actacctatt cgtgcagatt atgtggggaa aaatattcca acaagtcaat
    20221 ttgaagaaat tctagttgaa gtgatggaac atgatggtta cgatcgtgtg agtattattg
    20281 atccgagtta agtagacgac tattatttac gatgatttta tagaagggga agaactacaa
    20341 tggcaaaaaa attgttaatt ctagaggatg gtacggtttt tgaaggacta tcctttggtt
    20401 catctcttga tgtaactggc gaattagttt tttgtacggg gaataccggc tatcaggaaa
    20461 taataacgaa tccttcacat aatggaaaaa ttttagtttt tacctcaccg ctaataggaa
    20521 attatggtat tcatcgcagt tactctgaag ccattattcc cacttgttta ggagttgttg
    20581 ttgcagagta ttccagatgt gtaagtagcg atactagcaa gatgaatcta gatgaatttt
    20641 tgaaaatgaa aaaggttcct gcaatgtcgg gggtcgatac aaggtatttg atgcaagtta
    20701 ttaaagaaaa aggatttgta aaagctactt tagcagaagc aggtgatgtt ttatcacatt
    20761 tacaggatca attaatagcg acagtattac cgacaaataa tgtggaacaa gtttcgacaa
    20821 aaacagctta tccgtcgcct gctagcggtc gaaatattgt tgtacttgat tttggtctta
    20881 aacattctat tttgagagaa ttgtctaaac gtcagtgtga cgtgactgtc attccctaca
    20941 atacgtcttt agaaggtatt aaaaatctat acccagaagg tatcatatta tctaatggtc
    21001 cggggaatcc agaaaagctt caggaaatct taaacactat taaagaatta caaaaatctg
    21061 ttcctatgtt aggtattggt ttggggcacc aattaattgc catggcaaat ggtgcagaaa
    21121 ttatgagact cccagttgct aagaaggggc caaattatcc catgagagat attgctacgg
    21181 gtcgtttaga aacagttagt cagtttaatc attttaccgt taaccggcta aatcttccgc
    21241 atgatttact ggtaacacac gaaggtttga atgatcaaga gattgttgct ctaaggcacc
    21301 gctcatttcc agtgatgtcc gtgcaatttt acccagaggc tgctccaggc cctcatgatg
    21361 tgacctattt ttttgatgaa tttttagaaa tgattggatc acgcgcttaa acataaggta
    21421 ttatttaaag tcagtcggtg agctgattaa taaggaggtg cgtatatgct aaatacaaag
    21481 gataaacagc ctattttgct tattattttc ggacagaaaa tgaaagaata tgacgattta
    21541 attagatcaa ctgttgagag gttatctaaa agggatagac ctgttagtat tcttatctcg
    21601 aaggagctaa accatgtgat tcaccattat gatgcacaat ttgaaaatct tgagacgctt
    21661 gtggggtata taaatacaca tgaagatagt ttgatttacc ccctttgctt agataaaaat
    21721 catgagctat ttcaagagtt tttagcgtgg cagagtcatt attcaaggca gtatatcagc
    21781 gataatttat cagaaaaaag tgttcaggca atagacaata atcaagactt cgtgcaagta
    21841 gattgtttaa taatgagaga ctctttgaat aattgtttat atgtctctga tttagaatat
    21901 attgaattta ataaaacaac tggtaagtct atagcgattg taccaagtca aactttatcc
    21961 gatgctgcta ggcaaacaat tcgagatgtt gcatttgatg tgtgtcgaaa ggcaaacatt
    22021 attggagtgt gttattttag tttcttaata gatttgaaca gtttggatta tcatataatt
    22081 agcttatcat ctggtttgtc tcatcaaagc attttatttg agactattac gacctatcct
    22141 gttttagaga tagcaactaa attgacagtg ggatatactt ttagtcaact gaagcattct
    22201 tattacccaa atacctctgc ctttttagag ccacaattag actatgtagc tgccgtatct
    22261 tttagttttg aaaaagttga ttatatcttt tttgctcgta atatagagca attattttta
    22321 aatttactcg aggctagcag tcatgatcat tttttatttc tgagtgatat tagcgaggaa
    22381 gatttgatgt ttgctttgat tcagaaaaaa gaaaatcgtc tagcttatct tttagaggct
    22441 tttagacgtg gctttgattt gtgtgattta tcctcagtta ctaaaatcaa ccctttttac
    22501 ttagataaat gtttacatat tgtagagttg tatgaaaacc tgaataaaag ccaatataat
    22561 gtagatattt acaaggaagc taaaaggtat ggtttttcgg atgactatat tgctagtagt
    22621 tggcaaatct ctttgataga catgttagaa tatcgaaaaa aacatagtgt cgcacctgtt
    22681 ctgaagcaag tcgaacaaag ttctggtgtt ctaactggcc atcaaattca gtattttcga
    22741 agctatgatt ggcattcaga ctacattttt agcggttgtc aaaaggcttt gataatggta
    22801 gacaaaggtt attccctagg aaaattaaat gagttaatta agcaaatcaa acaaactcat
    22861 ttagaattat taattgttac caatcaacct ctcctcatag aacaattaaa tgatacatct
    22921 ataatttttg acactatagg tattgagacc atattaacaa ttatgggtat agaagaggtg
    22981 gaagaagtgt atttattggg agaaagtcaa gcagacatgt tttcaaaaat taaaaatttt
    23041 tctatctctt gtcagtctat ttagcaattc ttaataaaag ctgagatcta tatctcagct
    23101 tttatttgtg gtaaaaagta aagtactttc aatttt
//
LOCUS       DATRSD010000028        22780 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRSD010000028 DATRSD010000000
VERSION     DATRSD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.028"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBD85_000870"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBD85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO7133434.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBD85_000871"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBD85_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO7133435.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3369
                     /gene="nikA"
                     /locus_tag="VBD85_000872"
     CDS             1753..3369
                     /gene="nikA"
                     /locus_tag="VBD85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO7133436.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISL
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3356..4300
                     /locus_tag="VBD85_000873"
     CDS             3356..4300
                     /locus_tag="VBD85_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133437.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4300..5106
                     /locus_tag="VBD85_000874"
     CDS             4300..5106
                     /locus_tag="VBD85_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133438.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5094..5882
                     /locus_tag="VBD85_000875"
     CDS             5094..5882
                     /locus_tag="VBD85_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133439.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5869..6549
                     /locus_tag="VBD85_000876"
     CDS             5869..6549
                     /locus_tag="VBD85_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133440.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            6670..7398
                     /locus_tag="VBD85_000877"
     CDS             6670..7398
                     /locus_tag="VBD85_000877"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO7133441.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7414..7971
                     /gene="frr"
                     /locus_tag="VBD85_000878"
     CDS             7414..7971
                     /gene="frr"
                     /locus_tag="VBD85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO7133442.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8089..8943
                     /gene="cvfB"
                     /locus_tag="VBD85_000879"
     CDS             8089..8943
                     /gene="cvfB"
                     /locus_tag="VBD85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO7133443.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9081..9590
                     /gene="msrA"
                     /locus_tag="VBD85_000880"
     CDS             9081..9590
                     /gene="msrA"
                     /locus_tag="VBD85_000880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO7133444.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9587..9802
                     /locus_tag="VBD85_000881"
     CDS             9587..9802
                     /locus_tag="VBD85_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO7133445.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9959..11731
                     /locus_tag="VBD85_000882"
     CDS             9959..11731
                     /locus_tag="VBD85_000882"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO7133446.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11855..12841
                     /locus_tag="VBD85_000883"
     CDS             11855..12841
                     /locus_tag="VBD85_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO7133447.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12932..13735)
                     /locus_tag="VBD85_000884"
     CDS             complement(12932..13735)
                     /locus_tag="VBD85_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO7133448.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHNRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13927..14403
                     /locus_tag="VBD85_000885"
     CDS             13927..14403
                     /locus_tag="VBD85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7133449.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14725..15531
                     /locus_tag="VBD85_000886"
     CDS             14725..15531
                     /locus_tag="VBD85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7133450.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            15926..16423
                     /gene="ybeY"
                     /locus_tag="VBD85_000887"
     CDS             15926..16423
                     /gene="ybeY"
                     /locus_tag="VBD85_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO7133451.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            16404..16802
                     /locus_tag="VBD85_000888"
     CDS             16404..16802
                     /locus_tag="VBD85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO7133452.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            16844..17743
                     /gene="era"
                     /locus_tag="VBD85_000889"
     CDS             16844..17743
                     /gene="era"
                     /locus_tag="VBD85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO7133453.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            17980..18381
                     /locus_tag="VBD85_000890"
     CDS             17980..18381
                     /locus_tag="VBD85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133454.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFIIMKGCLTSISDILIYFYL
                     HLVVVYYTIGMILSLGRIISPEHSMFNKLRK"
     gene            18841..19092
                     /locus_tag="VBD85_000891"
     CDS             18841..19092
                     /locus_tag="VBD85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133455.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
     gene            19111..19365
                     /locus_tag="VBD85_000892"
     CDS             19111..19365
                     /locus_tag="VBD85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7133456.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLTFLLDLL"
     gene            complement(19704..19898)
                     /locus_tag="VBD85_000893"
     CDS             complement(19704..19898)
                     /locus_tag="VBD85_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133457.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            19897..20091
                     /locus_tag="VBD85_000894"
     CDS             19897..20091
                     /locus_tag="VBD85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133458.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(20142..20318)
                     /locus_tag="VBD85_000895"
     CDS             complement(20142..20318)
                     /locus_tag="VBD85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133459.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(20354..21946)
                     /locus_tag="VBD85_000896"
     CDS             complement(20354..21946)
                     /locus_tag="VBD85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO7133460.1"
                     /translation="MKIKKIISGLAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     EQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKLQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AATAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDAD
                     HLLDSQYNALAKDIPADRHQGA"
     gene            complement(22007..22075)
                     /gene="shp2"
                     /locus_tag="VBD85_000897"
     CDS             complement(22007..22075)
                     /gene="shp2"
                     /locus_tag="VBD85_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO7133461.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            22163..>22780
                     /locus_tag="VBD85_000898"
     CDS             22163..>22780
                     /locus_tag="VBD85_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133462.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTQLAINCLIISIDHSYFEHSHY"
BASE COUNT         7452 a         3393 c         4407 g         7528 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa
     2341 tgggttgtga aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac
     2401 tggggaaaga aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg
     2461 cgtgctttag cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt
     2521 ttagacactt ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct
     2581 atgtcaacaa gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt
     2641 cgtcaggcaa tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga
     2701 acagaaaagc cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta
     2761 aatccttata gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag
     2821 atgggaaaag ataaggttcg tgaaaaagat ggtaaagctc taaccttacg tcttccttat
     2881 atcgccacta aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa
     2941 atcggtatta atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa
     3001 aagggtaatt ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct
     3061 tggatgtcgg cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa
     3121 aatctagcaa ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag
     3181 gaagaaaatg ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta
     3241 tatatcccat taacttatca atcggtgatt tctctttata ggaaaggtga ttttaaaacc
     3301 atgcgttttg cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta
     3361 tctaagtagt tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc
     3421 acttctaaca tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg
     3481 tttatcaaaa ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt
     3541 agataagcca ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt
     3601 tggatattct tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc
     3661 tactttattt ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg
     3721 tgtgtgggct ggcttacatg aatctgctcg gagtgatcac ttaattcgat ttttgagttt
     3781 ttctagtgtg tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc
     3841 aaaattaaat cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag
     3901 tattacctta agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag
     3961 tcaagaaaat agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta
     4021 tattgtcaca catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt
     4081 gacttgggtt tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg
     4141 aataggacgt ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg
     4201 tatgctaatt tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa
     4261 ttgggttgat cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt
     4321 tcgagtagct tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc
     4381 tttgatccac aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg
     4441 ttagggactg accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat
     4501 tcactgtttt tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt
     4561 cttattgttg gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt
     4621 attttagctt ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt
     4681 ctagggaatt taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg
     4741 accaatttag ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga
     4801 ctttcagtct ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc
     4861 gttatggtcc ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta
     4921 gggattggtg tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga
     4981 tattttagga cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta
     5041 ttttccttta atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac
     5101 agttagaaat aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta
     5161 gttgtaaaat agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa
     5221 ccctcttggc aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca
     5281 tattttttaa gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg
     5341 gacgagatat tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa
     5401 ttgaagcgca tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct
     5461 tatctaaagc gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa
     5521 aaaaataccc ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat
     5581 tatctcttga tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata
     5641 attgttctac tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta
     5701 ctgtaacgca tgattatcag ttagctagag acctgggggg acaattatta gttataagtg
     5761 agggagaagt tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact
     5821 atacgaaagc actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt
     5881 aatcatgtcg gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt
     5941 aaaagaggag aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc
     6001 cgactcatta taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt
     6061 tacacaccta aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg
     6121 gtcaatccat attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaca
     6181 acttttgaat tatgtcaaat attagaagcg gtgggtttag atggaactta tctaaaatat
     6241 aaagctagac aacttagtgg tggtcagcta cagagagttt gtatcgctag agctttactc
     6301 ttaaagccta aaattattat ttttgatgaa tccttaagtg gattagaccc agtaactcaa
     6361 attaaaatgc tacgtttatt acaaaaaata aaacgccgtt atgaattaag ttttataatg
     6421 atttctcatg accctaaaat ttgtcaagct atctgtaacc gggtctttct gataaaaaat
     6481 ggttatttag tagaagataa tgaattcttg aagagagcgt gttctactaa ctgtttgacc
     6541 aatctctgaa ttattatgtt tttcttattt taggagttga taaagaaatc aacttactag
     6601 tgtcattgct ctattaaaat tttgcctttt atggtaaaat aataaagaca aaataaggag
     6661 aacatagtag tggaacctaa atatcaacgt atattaatta agttatctgg tgaagcattg
     6721 gcgggagata agggtgtcgg tattgacatt cctaccgttc aatctattgc aaaagaaatt
     6781 gccgaagtac acaattcagg tgttcaaata gcacttgtca ttggtggagg taatctttgg
     6841 cgtggagaac ctgcagcaga agcagggatg gatcgtgttc aagctgatta tacagggatg
     6901 ttagggacag taatgaatgc tcttgtaatg gctgacagcc ttcaacaata tggggtagat
     6961 actagagttc aaacagctat tccaatgcaa actgttgctg agccttacgt acgtggtcgt
     7021 gctttacgtc atctagagaa gaatcgtatt gttgtctttg gagcgggtat tggttcgcct
     7081 tatttctcaa ctgatacaac agcagcgctt cgtgctgctg agattgaggc agaagcaatt
     7141 ttaatggcta aaaatggagt agatggtgtt tataatgctg atccaaagaa agatgccaat
     7201 gctgtcaaat ttgatgaatt aactcacgtt gaagtaataa aacgagggtt aaaaatcatg
     7261 gatgcaaccg catcaactat ttcaatggat aatgatattg accttgttgt tttcaatatg
     7321 aatgagactg gtaatattaa gcgtgttgtt cttggagaac aaatcggaac aactgtttca
     7381 aacaaagcat ctgaatagga gtgtataaaa aatatgacta aggaaatcgt tacaaaagct
     7441 caagagcgtt ttgagcaatc acaccaaagt ttatcaagag aatttgcagg gattcgtgca
     7501 ggacgtgcta atgctagctt gcttgatcgt attcaagttg agtattatgg agcgccaacc
     7561 ccccttaacc aattagcttc tattactgta cctgaagctc gtgttctttt aatttcacca
     7621 tttgataaat catcaatcaa agatattgag cgtgctatta acgaatcaga tttaggtatt
     7681 aatcctgcaa atgatggttc agttattcgt cttgttattc cagctttgac agaagaaaca
     7741 cgtcgtgatt tagctaaaga agtgaaaaaa gtcggagaaa atgctaagat tgctatccgt
     7801 aatattcgcc gtgatgctat ggatgaagct aagaagcaag aaaagaataa agaaattaca
     7861 gaagatgact tgaaatctct tgaaaaagat attcaaaagg ctactgatga tgctgtaaaa
     7921 catattgatg agatgactgc taataaagaa aaagaattgt tggaagttta atagtaacgt
     7981 ttgctatctt tattatgatt ggcaagtaat aaaatgatta tggaggtaac tgatagaatc
     8041 aaaaccagat ggatttggtt tgtcggttac ctttttaaaa ggagaaaaat gaatacatta
     8101 ttagcgacag tcataacagg cttagtaact gatgaaaaca aagattttta ctttatccaa
     8161 aaagatggct ttacctttgc attatcaaag tcagaaggag agcatcacat tggtgaaatg
     8221 gttaaaggat tcgcctatac agatatgcaa caaaaagcac gtcttacaac taaagaaaca
     8281 tttgcgactc gagatcatta tggttggggg acagttactg aggtacgtaa agacttggga
     8341 gtatttcttg atactggtct tccagataag caagtggttg tttctttgga tgttcttcct
     8401 gaattgaaag aactttggcc taaaaaaggt gatcgcctct acgtttgctt agatgttgat
     8461 aaaaaagatc gtctctgggc tctccctgca gatcctgaag tgtttcaacg aatggcaacc
     8521 cctgcatata acaatatgca aaatcaaaat tggccagcaa tcgtttatcg cttaaaattg
     8581 tcaggaactt ttgtttatct accagaaaat aatatgctcg gctttattca ccctagtgaa
     8641 cgttacagtg aacctcgttt agggcaagtg ctggatgcac gagttattgg cttcagagaa
     8701 gttgatagaa ccttaaattt atcattaaag cctcgttctt ttgaaatgtt agaaaatgac
     8761 gcacaaatga ttttaacgta tttagaaagt aacggaggat tcatgacttt aaatgataaa
     8821 tcttctccag aggaaattaa agctactttt ggaatttcca aaggtcagtt taaaaaagca
     8881 cttggaggac tcatgaaagc taaaaagatc aaacaagatc agctaggtac agaattatta
     8941 taaacttttg aacttttaaa gttttctcac gaggtcgttt tagttatagc tagaattaat
     9001 ggctaattat agaaaaaagc tgagaggaaa ttttggcttt ttttgttata atgtatctat
     9061 taaaaagaat ggagaacgat atggaacgtg cgatttttgc tggaggttgt ttttggtgta
     9121 tggtacaacc atttgaagaa ttagatggta ttgaatctgt tttatctgga tacactggag
     9181 gacatgttga gaatccaact tataaagaag tttgtagtaa aactactgga catacagaag
     9241 ctgttgaaat tatttttaat cctgaaaaaa tctcttatgc agatttagtt gagttatatt
     9301 gggcacaaac agatccaaca gacgcttttg gtcagtttga agatcgtggg gataattatc
     9361 gtcctgtcat cttttatgag aatgaagagc aacgtcagat tgctcaaaaa tcaaaagata
     9421 agctacaagc ttcgggtaga tttgatagac caattgtaac cagcattgag cctgctgata
     9481 ctttttaccc agcagaagac tatcatcaag cattctatcg gactaaccct gctcgttacg
     9541 ccttgagtag tgccagaaga catgcatttc tagaggagaa ctggcattga gaaaatcatt
     9601 ttacagttgg ttgatgacac aaagaaatcc aaaatcaaat gaaccagtag caatcctagc
     9661 agattacgct tttgatgaaa ccacttttcc aaaacatagt tcggattttg aaacagttag
     9721 tcgctattta gaagatgagg ccagtttttc ctttaattta acagattttg atgatatttg
     9781 ggaagattat ctcaatcatt aacaataata tagagattgt attttcaatc tcttttttag
     9841 gttatagaaa ttggacacat tgtctaagac tgtctgaaaa gcaggttttt taatggcaat
     9901 cgttttcaca aaggagtaga atatagtcat ctaaaatata gccctaggga ggttgtttat
     9961 gagatataca aatggaaatt ttgaagcctt tgcaagacct cgaaaacctg aaggtgtgga
    10021 taaaaaatcc gcttatattg ttggttctgg tttagcagga ttagctgccg ctgtcttttt
    10081 aatacgtgac ggtcaaatgg atggtcaacg tattcatatt tttgaagaac tacctctttc
    10141 tggaggatca cttgacggtg tcaaacgacc tgatatcggt tttgtaacgc gtggtggtcg
    10201 tgaaatggaa aatcacttcg aatgtatgtg ggatatgtac cgttccatcc cctctctcga
    10261 agttccagat gcttcttatc tagatgaatt ttattggctt gacaaggatg atcccaattc
    10321 atctaactgt cgcctcattc ataaacaggg gaatcgctta gaatctgatg gtgattttac
    10381 actcggaaca cattccaaag agttagttaa gctagtcatg gagactgaag agtctttagg
    10441 tgctaagacg attgaagaag ttttttcaaa agaatttttt gaaagtaatt tttggactta
    10501 ttgggctact atgtttgcct ttgagaaatg gcattcagcg attgaaatgc gtcgatatgc
    10561 tatgcgcttt atccatcata ttggtggtct gcctgatttc acttcattaa aatttaataa
    10621 atataatcaa tatgattcta tggtgaaacc aatcatcagt tatttagagt ctcataatgt
    10681 agatgttcaa tttgatagca aggtaactaa tatctccgta gactttaaga acggtcagaa
    10741 gcttgcaaaa gctattcatc tcacagttgg tggtgaagca aaaacaattg atcttactcc
    10801 aaatgatttt gtttttgtaa cgaatggttc tatcactgaa agtacgaact atggtagtca
    10861 tgatactgta gctaaaccta ataccgattt gggaggttct tggaatcttt gggaaaactt
    10921 agctgctcaa tctgatgaat ttggacatcc taaagtcttc tacaaagata ttccaaaaga
    10981 gtcatggttt gtttctgcta ctgcaacaat aaaagatcca gctattgaac cttatataga
    11041 gcgtttaaca catcgtgacc tccatgatgg taaagtaaat accggaggca ttgttacagt
    11101 tacagattct aattggatga tgagttttgc tatccatcgc caaccgcatt tcaaagagca
    11161 aaaggaaaat gaaacaattg tttggattta tggactctat tcaaatgtgg aaggtaatta
    11221 tattaaaaaa ccaattgaag aatgtactgg tcgagaaatc acagaagagt ggttatatca
    11281 cttaggtgtt cctgaaatga agattcatga tttatctgat aaacaatatg taagtacagt
    11341 tcctgtttat atgccttaca ttactagtta tttcatgcct cgagttaaag gtgaccgacc
    11401 agatgttatc ccacaaggat ctgttaacct agccttcatt ggtaattttg cagaatctcc
    11461 atccagagat acagtattta caacggaata ttctattaga actgctatgg aagctgttta
    11521 tactttctta aatattgaac gtggtgttcc agaagtcttt aattcagcat ttgatattcg
    11581 tgtcttgttg caatctttat actatctcaa tgataagaaa tctgttgaag atatggattt
    11641 accaatacct gccttaatgc gtaaagtagg catgaaaaaa attagaggaa cttatttaga
    11701 agaattactt cgagaagctc atcttttata atgtaaaata aaatcgagaa aacttgtttt
    11761 tctcgatttt ttagttttac aacgactaaa atcaccaaag aaaatgaaat tttgttataa
    11821 tgtagtcact tactgtacga aaaggaagaa taatttgcaa gaatactcta tcgaaattac
    11881 tttacaacat ccagatgata tgatgtcact ctttggcagc aatgagcgcc atttaaaatt
    11941 aatcgaagaa aatttagatg ttatcattca tgctagaact gaacgtgttc aggtattagg
    12001 tgactctgaa gaagcagtag aaaccgctcg gttaaccatt gaagcattat tagtgcttgt
    12061 taaccgcggg atgactgtga atacttcaga tgttgtaaca gccttatcca tggcacaaaa
    12121 tggttcaatt gataaattcg ttgcccttta tgaggaagaa attattaagg atagctatgg
    12181 taagcctatc cgtgttaaga ctttagggca aaaaatatac gttgatagcg ttaaaaatca
    12241 tgatgttgtt tttggtattg ggcctgctgg aacgggaaaa acttttttag cagtcactct
    12301 agcagtaacg gcactaaagc gtggtcaagt taaacgaata atcttaacga gaccagcggt
    12361 tgaagctggt gaaagccttg gtttcttgcc tggagatctc aaagagaaag ttgatcctta
    12421 tttgagacca gtttatgatg ccctatatca aattttaggt aaagagcaaa caagtcgctt
    12481 aatggagcgt gaaatcatcg aaattgctcc attggcttac atgcgtggtc gtactttaga
    12541 tgatgcattt gtcatattag atgaagcgca aaacaccact ataatgcaaa tgaaaatgtt
    12601 cctaacacgt cttggcttca attctaaaat gattgtaaat ggcgatgtta gccaaattga
    12661 cttaccaaag aatgttaaat ctggtttgat tgatgctgtt gagaaattac gaaatatcaa
    12721 aaagattgac tttattcatt tatcagcaaa ggatgttgta agacaccctg tcgttgctga
    12781 aataataaat gcttattcag attcagaaag tagtcacaag ctacaatctg ataaggactg
    12841 atgtttgcca ctaggagacg ttgttagaga gtgtgtcaat atgtaaaaaa cgcctaatgc
    12901 tttaggcgtt tttatgatgg tgtacttcaa cttaacctat aaagtgttga aaattttctt
    12961 taagcatgac gtcgagatgc attattatat cgtttaatgt ttctttaaca gtgtgttcgt
    13021 tggagttgtg atgacatagt ttaatgatat tttcttcgaa atgtttagga agcaatgtag
    13081 cattcttctt agcataggaa atcattcttt tttcgccagg atgtgtcaac ttattaagtg
    13141 caaaaataat atcaaagtag gaatctaaga aagcagtagt tctattgtga gtactaacaa
    13201 agtcttggcg tttaagggct tttataattt gtttatcgta agagggaagc ttgccagtta
    13261 ataagttacg attttgaatg ataatttgtt tttgtaactg actgggataa gaaacttcga
    13321 atctctcttt gagtgattgt aattgatttt caggatcata gagtatttgg caattgatga
    13381 ggttatgcca aaaacaagta gtgtatccaa ttcgagaatt gtggtgttca acgacatctt
    13441 ctaagtctga taaaaagtta tctatgttac gatagagaat atcaatatca gttccgtcgt
    13501 ttaaagtacc attatcttca agctcccagt aatgattgtt aagttcaatg taatgacagt
    13561 gcttgttaag gatacgttta cgactagtga tatcaggagt tgctgcacaa taaacataca
    13621 aatcataatc ggaagattgg tcaaaactgt caccagagcg tgagcctccg agtgctatag
    13681 caacaacatt tggaagttta gagaaatctt gccagagttt tgtgaaatgg tccataaatg
    13741 ctctcctaaa aatgtaaaat attgatgtgt ctccattcta cagggctatc tctttttagt
    13801 caataattaa tagtaagtta ataaaagagg taacggttct aatattcaag aagtgtatat
    13861 gagtgatttg aaaaggagat attttatgct acaatacgta catattagtt taaacaaggt
    13921 gatagaatga aacaagatta tatttcctac attaggtcaa aagttggaca tgagactatc
    13981 tttttaacat attctggtgg tattttgaca gatggtaagg gacgtgtctt actccaatta
    14041 agagctgata agaattcttg gggaattatt ggtggatgta tggagttagg tgagtcatca
    14101 gtagatacat tgaaaagaga atttttcgaa gaaactggat tgagagtgga accaatcaga
    14161 ctgttaaatg tttatactaa ctttcaagac tcttatccca atggcgataa agcacaaacc
    14221 gtcggtttta tttatgaagt aagctgtcca aaaccagtta atatagaagg ttttcataat
    14281 gaggaaacat tgcaattgga ctatttttct aaagaagacg tcaaaaatat tacgatcgta
    14341 aatgaacaac accaattaat tttagatgaa tatttttcac aaacattcca aatggggcgt
    14401 taaaaggatg ggaaatacta ggtgtgactt taaagaactt ctaactttta ttgatatgtt
    14461 gttaggcatt cttgtagaaa caaatgttaa gaaaataagg agttctcatt tgtatatttt
    14521 ataattatta aaaagaaaaa cagtagcttg caataattta gtgactgttt ttttagaaag
    14581 cataagaagt acagaaaaag aaaattatgg aatatttgat ggtggggata tgctttactt
    14641 acggtattgg aatgatatac ttaaagaaat ccagttttag aaatccaaat ttctaagtaa
    14701 aatagcataa aggagagtag atgaatgaag ataacattac acggcgttgc tgaaacgtta
    14761 ttaatcacct tatatattag ggctaaagat gcgatggcta aacatccgat acttaatgat
    14821 caaaaatcct tagcaattgt tgaacagata gaatatgatt ttgataaatt cgataattca
    14881 gaagcttctt tttatgcaac attagctaga attcgcgtta tggatagaga aatcaaaaaa
    14941 tttattagag aaaatccaaa tagtcaaatc ctttcaattg gttgtggact tgatacaagg
    15001 tttgaaagag tcgataatgg acaaattagg tggtataacc ttgatttgcc agaggttatg
    15061 gagataagaa aattattttt tgaagagcat gaaagagtta ctaatatagc aaaatcagcc
    15121 ctagatgaaa cttggacacg ggaggtaaat ccccaaaatg ccccttttct aatcgtgtca
    15181 gaaggtgttt taatgtttct aaaagaagat gacgtagaga cttttcttca tatcctgaca
    15241 aattcattta gccaatttat ggcacaattt gatttgtgtc ataaggaaat gattaataaa
    15301 ggaaagcaac atgatacagt aaagtatatg gatacagaat ttcagtttgg tatcacagat
    15361 ggtcatgaga ttgtggattt agaccctaaa ttaaagcaaa taaatctgat taactttaca
    15421 gatgagatga gcaaatttga gttaggcaca cttcgctctt tacttccaac aattcgtaaa
    15481 tttaataatt gtttaggtgt gtacgaatat aaagcatctg agaaaaagta acaaaaatct
    15541 atttatataa agagacattc taaatgtaaa tttgtcataa tcgttatagc atttgtagct
    15601 ttgtttattt aggaattagt tttacgtaag ttaggcgctc taaagtttgt attttgaagt
    15661 aaatactaat tcataagtca gaattttatt gaaatggatt ataagtgtgt aatagtgatt
    15721 attagctttc tctccctttt ttaatactct tatggtataa tgatgtgaaa gaatagcttg
    15781 aaggaaggaa gagaacgcac ttttattttg agaactagtt ttagataaaa gtgaaaaact
    15841 taggaaagta gatgtgtcga ttatttagaa ttatgcatta cactttatct tttctaaacg
    15901 ttttgggtcg ttcttactat ataagatgta cgttgaaatg attgatgaaa ctggacaagt
    15961 ttcagaagat atcaaaaagc aaactcttga tttgttagag tttgcagcac aaaaaacagg
    16021 taaagagaat aaagaaatgg ctgtaacgtt tgtcactaat gaacgtagcc atgaattgaa
    16081 tttagagtat cgagataccg atcgtccgac tgatgttatc agtttagaat ataagcctga
    16141 ggttgatatt tcttttgatg aagaagatct ggctgaaaat cctgaacttg ctgagatgct
    16201 agaagatttt gattcttata ttggtgaact ttttatttct attgataaag ctaaagaaca
    16261 agcagaagag tacggtcact cttatgaacg tgaaatgggt tttttagctg tacatggttt
    16321 tttgcatatt aacggttatg atcattatac acccgaagaa gaaaaagaga tgttcagctt
    16381 acaggaagaa attttaactg cttatggact taaacgacaa taatcaaaaa aaatggaaaa
    16441 atcgcacctt aacatcaagc atggaatttg ctgttacagg gatttttaca gcttttaaag
    16501 aagaaagaaa tatgcgtaag catttggttt ctgccatttt agtaatactt gctggtctta
    16561 ctttccaagt atctatggtg gagtggttat ttcttttatt gagtattttt ttggtaatta
    16621 cttttgaaat tatcaattca gctattgaaa atgtagttga tttggcaagt aattaccatt
    16681 tctcgatgct ggctaaaaat gctaaagata tggcagcagg agcggtttta gttgtttcct
    16741 tatttgccgt attggtggga ttgattattt ttatacccaa gatacttgct ttacttttat
    16801 gatgagaagt attacgaata aattgaatgg agaaagaaaa gaaatgacat ttaagtcagg
    16861 atttgtagca attttaggtc gtccaaatgt tggaaaatca accttcctaa accatgttat
    16921 gggacaaaaa attgctatca tgagtgataa agctcaaaca acacgtaata aaattatggg
    16981 gatttataca acagaaactg agcaaattgt tttcattgat accccaggaa ttcataaacc
    17041 aaagaccgcc cttggtgatt ttatggttga atctgcttat agtactttac gtgaagttga
    17101 aacagttctt ttcatggttc ctgccgatga aaagcgaggc aaaggcgatg atatgattat
    17161 tgaacgtctt aaggctgcca agattcctgt tattttagta atcaacaaaa ttgataaggt
    17221 tcatccagac caacttttgg aacagattga tgacttccgt agtcaaatgg attttaaaga
    17281 agtagtgcca atttctgccc ttcaaggaaa taatgtgcca accctaatca agttattgac
    17341 tgataatctt gaagaaggtt tccaatattt cccagaagat cagattactg atcatccgga
    17401 acgattttta gtatcagaaa tggttcgtga gaaggtactt catttaacgc aacaagaggt
    17461 tcctcactca gtagctgttg ttgtcgaatc catgaaacgt gatgaagaaa ctgataaagt
    17521 gcatattcgt gcaactatca tggttgaacg tgatagtcaa aaaggcatta tcattggaaa
    17581 acaaggtgct atgctcaaga aaataggaaa aatggcgcgc cgtgatattg aacttatgtt
    17641 aggtgataaa gtttacctag agacttgggt aaaagtcaag aaaaactggc gtgataaaaa
    17701 gcttgattta gctgattttg gctataatga aaaagagtat taatgagttt attttgagat
    17761 ccgcaagtta gtggggaaga gttagataag ttcaccgtga gacttgattc gcacagaaaa
    17821 atcaattcac gaggataaaa ccaactagtc ataattttga gattatattc acaaattaat
    17881 taaagggagc caaacatgct tgttggcccc ttcttaaata aaggagagca catgactcaa
    17941 gattatattt gctacctata aagtagagga ggtgaggata tgcataatat attaagattt
    18001 ttaggcattg ttattatttc tgcagtgatt ctatttagta taggtagttt ctacgattta
    18061 tcattaatga aaaatatact acttatctgt tggagttttc tctttgatct attggttttt
    18121 gtcttcaaac aaagacaaac gacagaagta ctcacatggt atcaagttgt taagcaattt
    18181 tggttattta ttaagtgtac tattcttatt cctatcttag ttgcttttat tataatgaaa
    18241 ggatgtttga caagtatatc agatatatta atttactttt atttacacct tgtcgttgtt
    18301 tattatacga taggaatgat tttatcatta ggtcgtatca tttctcctga acactctatg
    18361 tttaataaac ttagaaagtg aaatgaatta tatttaaaat ttgttttcaa cagagcggat
    18421 ttgaccatct gttgtttgcc atgcctttca taatttttca gtaattatga tagttgttct
    18481 gttctcccaa tcataaaact ttaaataaga ttctattagt tcttgcctca ctctccttaa
    18541 gtttttggta aaaatcgaaa agttaccaga ctttaaaggg gagtttttta tttgtcaaca
    18601 gttagtagaa aaacgatata ttgctccatt tagattaatt atggagataa ccaaataata
    18661 gcttgtgata agaagatacc tttgaagttt cagaaacaat ctgttttcca tcctgctata
    18721 ttcatctttg ccaaagttgc aacaacacaa taataacaat tgcttgggca ttgggtgatt
    18781 agtttactat caatagggtt agagtggagt tggagtatat ggattggcat taatcttata
    18841 atgatgattc tgttgagtta tgtgctaaca aaaactgagc caatcagtaa ctaccaagag
    18901 tcatcatcta acctcaaaaa atggctattt gcaataggtg cttttagcgt tttattttgc
    18961 tgggcactac tagtaaaaac atttttccca ataaggcaaa accaggaggg attaatagct
    19021 gatatgaatc aggtcaatca ctactttcga gttgtcttat tattgactat ctgtcttatt
    19081 gccccaattt aagaagagat aacgttcagg ttgtttgtca tgagaacatt tgggaactcc
    19141 ctgagtggtc ccttggttgt gatcttatct tctatactat ttagctggtc tcacttacat
    19201 ggtctatctg tcgtcgactt tgttgtctat tttggtatgg gtttgatttt cgctagtttg
    19261 caccactata caaaatccat tcattattcg attggcgagc atattgtttg gaatagtttg
    19321 tcatatattt tttattttct aacatttttg cttgatttac tctaagtaat aagattgaga
    19381 gactaatcac attactaaga catttttgtc agtttaaaag agctttttgg ctcttttgtt
    19441 attcgttttt ttaatgaata aaatgttatt attttattgt taactaaata aagttcgtaa
    19501 ctttatatga tttatataga attacgagtt ttttgttaga taaatatgag aaaatatagt
    19561 atgacgaatt cctaaataaa aaataatgaa aggaacatga tgttaaaaga tgttaatgtt
    19621 ggaatgagtc atttattagt tagaggtgct attattctag tagtagggat tgtttttctg
    19681 ttgcaacaga ttcagtaagt acttcaagtg ggagctttgg aataggttat cgagaagaaa
    19741 agacccagac gttctctttg gatcagctgt cccaagtgat tttgtattgc ggggagccca
    19801 atactgttag atttacttat acaaatagct tctatatgat gaaagatatt tatatcgctc
    19861 tggatggtgc tggtagctct ttgaccactc atgatcatgc tgttcctaat gattaaacca
    19921 gattatataa ggtatttcaa agtcattgac gatatgcatg ataccatgta ccaacagact
    19981 gtttttcatg ttcaactgca agcttggaaa tacaccatga aatcgattga tcaattagtt
    20041 gtagatgatt tgaatcatat cagtaatgag aaaaccactt tggttaaata aaaaggaaca
    20101 ctgtcaaaaa tggcagtgtt cctttttatt ttaggagaga gctaagattg gtcatctaat
    20161 gggatttcca catgttcaac tgtcatagta atgacaggtt tgataacaca atctctgtct
    20221 ttagggatag tctcctgttg cagaggcgcc tcatcgctcc aaattttctt gtcagaacaa
    20281 ttggaatgta gataatttga atcagtcttt ttagacattt catatccttt ccaggctcaa
    20341 tacaattttt gagttatgca ccttgatggc gatcagctgg aatatcttta gctaatgcat
    20401 tgtattgact atccaaaaga tgatcagcat ccattattgt tttaccaaca aggaaatatt
    20461 tattgctttt tatattatta tcccatgttg tatcaacata atatttaacg ccattaatag
    20521 taacaatgtt ccaagcgtga tttccacctc cagcttgacc agttacatac catactgata
    20581 agccagcagt agcagccata tctttaaaca ttactgcaaa ggcttgacaa acaccacgtt
    20641 tatctttgta gagtgcttca ggagcatgga tactgatacc actagcagtt tcacgagtgg
    20701 ttgccagttc ttcagtcgcg taagtgtaat tattaaccaa gtggtcatga attactttag
    20761 ctttttcata atcacttgtg atatttttgt tagtaatatt ttctttgaca aaagaatcta
    20821 caaactgttt atattcttta tgtaatccta ccatgtcacc tttaagagta taggtgattg
    20881 tgatgtcgct ttttacaata atgcgttgga tttgtccacc aagagtataa gtgctaatgt
    20941 tacttttggc attgaattca gttgctgttc caagtgtatc tccaatttcc cggatgatgc
    21001 ttccgagatt tttttggtat tcgtcaactt gttcttgagt tttaaactcc atttgaacac
    21061 tgatgtgtgg tacttggttg ctaatattat ttcgtaaaac ttcttttaaa ctatcgtaat
    21121 cggcaatatt agctgactca ggctgtgatg cctttgcatc agaggtagcc tgtttttcct
    21181 cactcttagt tacttcttgt ttattgcctg acgcttctga ttgagtagtt ttttcttcta
    21241 ctttgtttgc aggaacagaa gtattttcgc taactttgag agtagcaagt ttagaagtct
    21301 cagtttcaat agccgctgtt gatagggcat ctacttcttg ttgaaggatt gccatagcag
    21361 ctttgtaatt attaacttca gctacaagag gttcaatttg atttctgagt gaatgaaata
    21421 aattttcagc ttcttgtata gaattcacag agttgtagtt tacagttgct aaaatagctt
    21481 gaattttgtt aacaacctga ttacgttgcc ccacgagctg agctgcttta ttttgacttg
    21541 tctgaagctt attatgattt tggtcaatca gattttgaac agtttggtta atgacattac
    21601 gttgattaac gatagcatta tagttactgg gagagagatg accagctttc acctggttaa
    21661 ataattgctc atctttttgt ttaaataatg ttatagctct atttaactgt tcaatagttg
    21721 gattagcatt tagttgagtg aatgagttga cattatcaac aacaaattga ttgagcaaac
    21781 gatgttgttc cttgatgtaa ctaataagct gctcttcatt attttgctta ctgatatagt
    21841 gatactcact ggtggtgtca tcagctttaa cctcatagtt aatagttgat agtgaactga
    21901 taattaaagc tgcggcaagg ccactaataa tttttttaat cttcattagt tctcctgtta
    21961 taaactaaat aaaatgggat tccataacaa ccatttgtgg ggagttttat ccaccaacga
    22021 taattaggat atccatgatt aatgtgaaca acaaagcttt gttaattttt ttcatgacta
    22081 tctcctttct gattttctat tttgtacttg tattatataa tggatttaaa gggtctttta
    22141 aattgttgtg aaagtgggaa aaatggaaaa agaattagga aaaacactaa gaagattacg
    22201 taaaggaaaa aaagttagta tttcttcttt agcagatgag cacctatcca agtcacaaat
    22261 ctcaaggttt gagagaggag agtcagaaat tacttgtagt cgtttgctta atattttaga
    22321 caaactcaac atcactattg atgaatttgt gtcgattcat tctaaggcgc acactcattt
    22381 ttttatcctt ttaaatcgag taagaaaata ttgtgctgaa aaaaatgtaa ctaaattagt
    22441 ggctctctta gaggatcata accataaaga ctatgaaaag attatgatta aggctcttat
    22501 tttttcaatt gatcaatcaa tagagcctaa tcaagaagaa ttagcacgct taacagacta
    22561 tctttttacg gtggagcagt ggggatatta tgaaattatt ctcttaggaa attgctcacg
    22621 tctcattaat tacaatactt tatttttatt gaccaaagaa atggttaatt cgtttgctta
    22681 ttcagaacaa aataaaacca ataaaatcct agttacacaa ttagcaatta attgtctaat
    22741 catcagcatt gatcactctt actttgagca tagccactac
//
LOCUS       DATRSD010000029        22549 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRSD010000029 DATRSD010000000
VERSION     DATRSD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22549
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.029"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            110..916
                     /locus_tag="VBD85_000899"
     CDS             110..916
                     /locus_tag="VBD85_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7133463.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            999..1502
                     /locus_tag="VBD85_000900"
     CDS             999..1502
                     /locus_tag="VBD85_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO7133464.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1606..1782
                     /locus_tag="VBD85_000901"
     CDS             1606..1782
                     /locus_tag="VBD85_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO7133465.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(1879..2256)
                     /gene="mscL"
                     /locus_tag="VBD85_000902"
     CDS             complement(1879..2256)
                     /gene="mscL"
                     /locus_tag="VBD85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO7133466.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            2380..4254
                     /gene="dnaG"
                     /locus_tag="VBD85_000903"
     CDS             2380..4254
                     /gene="dnaG"
                     /locus_tag="VBD85_000903"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7133467.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            4262..5371
                     /gene="rpoD"
                     /locus_tag="VBD85_000904"
     CDS             4262..5371
                     /gene="rpoD"
                     /locus_tag="VBD85_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO7133468.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            5480..5821
                     /locus_tag="VBD85_000905"
     CDS             5480..5821
                     /locus_tag="VBD85_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO7133469.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            5911..6765
                     /gene="rfbD"
                     /locus_tag="VBD85_000906"
     CDS             5911..6765
                     /gene="rfbD"
                     /locus_tag="VBD85_000906"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO7133470.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            6882..8036
                     /locus_tag="VBD85_000907"
     CDS             6882..8036
                     /locus_tag="VBD85_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO7133471.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSFDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWAK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            8026..8967
                     /locus_tag="VBD85_000908"
     CDS             8026..8967
                     /locus_tag="VBD85_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7133472.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            8969..9700
                     /locus_tag="VBD85_000909"
     CDS             8969..9700
                     /locus_tag="VBD85_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7133473.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            9697..10050
                     /locus_tag="VBD85_000910"
     CDS             9697..10050
                     /locus_tag="VBD85_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO7133474.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            10043..11776
                     /locus_tag="VBD85_000911"
     CDS             10043..11776
                     /locus_tag="VBD85_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7133475.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIILN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNTDKKVSLYQPNGSWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            11801..12625
                     /locus_tag="VBD85_000912"
     CDS             11801..12625
                     /locus_tag="VBD85_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO7133476.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIVYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGRYMELPPESKRESHSYYTWYWKEG"
     gene            12625..13347
                     /locus_tag="VBD85_000913"
     CDS             12625..13347
                     /locus_tag="VBD85_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO7133477.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            13347..14405
                     /locus_tag="VBD85_000914"
     CDS             13347..14405
                     /locus_tag="VBD85_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO7133478.1"
                     /translation="MMIKFSNKIVQEDLALISDQNYINWERLANKAVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEGRLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            14407..15342
                     /locus_tag="VBD85_000915"
     CDS             14407..15342
                     /locus_tag="VBD85_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7133479.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHGLEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHVTGLKTFVDFYEIVQKVVNKYQNDVALNQRLAFEKERLAA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            15351..16298
                     /locus_tag="VBD85_000916"
     CDS             15351..16298
                     /locus_tag="VBD85_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7133480.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLLDAKRLSVDYLFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGISGVIGSGYNLNRGVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            16300..17664
                     /locus_tag="VBD85_000917"
     CDS             16300..17664
                     /locus_tag="VBD85_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO7133481.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGSILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            17664..19088
                     /locus_tag="VBD85_000918"
     CDS             17664..19088
                     /locus_tag="VBD85_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7133482.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDVLVHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSAGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAAESLMIICISY
                     GNALGTQYLLASRRTKAYTISAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            19085..19933
                     /locus_tag="VBD85_000919"
     CDS             19085..19933
                     /locus_tag="VBD85_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7133483.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLHLPKVGMLSCLYQDMNRENLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRYKYLVFMRNEFVRVLVAEEQKSKKIVAMIKGLKDGLLMK
                     VDKYGK"
     gene            19923..21062
                     /locus_tag="VBD85_000920"
     CDS             19923..21062
                     /locus_tag="VBD85_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7133484.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHGDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKYVSDENIDFVICNT
                     RFQLTTLEGLSFAKNHHLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFAGTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKN"
     gene            complement(21114..>22549)
                     /locus_tag="VBD85_000921"
     CDS             complement(21114..>22549)
                     /locus_tag="VBD85_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133485.1"
                     /translation="CHQLGLSELLIRKYCHDLTTLFNSQLSLNIEKSTIVYQSNGVTR
                     EQAFKYIYHQSHVLQLLKFLITNDSGRLPLTYFSEKFGLSCATAYRIRKHISPLLEKL
                     GFQIVKNTITGDEYRIRYLIAFLNAQFGIEVYPMSKMDKLLIKRLLLEHSTTFTASHY
                     FPNTFIFFDTLLSLSWKRINYNVVVPYSSLFTELQNIFIYDTLQYCVKNVIIDSFKIN
                     LKKDDIDYIFLAYLTSHNSFSNPNWTEKRIDNVIAIFENYPKFQKLLQPLKDALPLSG
                     SYHDELVKVAIFFFRTFILGLNQLIPETISFPSWNYHRHDKLTTILRPIITNWLSKIG
                     EYTFKEQHFLLLCTHLERIIKNHIPPIQIAVLTTDFINNQILTECLLQRFSSKKIHFH
                     PYYLLTDDLSNITNLNPDIIITNKKLSPFIKHEISSESLITYIDLINTSDQINQIQKI
                     ISSIQEEKYCKLFAKTNEITNRSSYNS"
BASE COUNT         7636 a         3246 c         4365 g         7302 t
ORIGIN      
        1 agcaagcata gttatcggtg taatttactg aatttttatt caaaaaaact tgacttatgt
       61 ataaagatga tttatacttg ttcctatcat aataaaagga agatagttta tgtcaaaaaa
      121 aataatatta ggaattttat ctcttttatc tgtcgttact ttggtggcgt gtggttcatc
      181 agacaaacag ctacaagata aagttgagaa aaaagggaag ttagttttag cggtgagtcc
      241 agattatgct ccctttgagt ttaaagcgct tgttaatggg aaagataaag ttgtcggcgc
      301 agatattgag ttagctcaag ctattgctga tgagcttcat gtcaaactag aggtatctcc
      361 tatgagtttt gacaatgttt tatcaagttt acaaacaggt aaggctgata tggctatttc
      421 tggattatca tatactaaag agcgtgctaa agtctatgat ttttcaaaac cttattacac
      481 tacagaaaat gcggttcttg ttaggaagtc tgatttagga aaatatactt caatcgaaag
      541 ccttaaaggt aaaaaaatag cagtacaaaa aggaagtatc gaagagggag tttctaagaa
      601 atcattcaaa aattctcacg tcgtgtcact gacagcgatg ggagaagcga ttagtgagtt
      661 aaaatcaggc aaggtagaag ctgttgattt agaaaaaccc gtagctgaag gttacttggc
      721 ccagaatcca gaccttgttc ttgctaagtt tgcccttaaa acaggagaag gggatgctaa
      781 ggctgtagct ttacctaaag acagtggaaa aatgttgaag actgttaaca aagtgataaa
      841 gagacttgaa aaagaagata aatataaagt ttatatttca gatgcggcta aattaactgg
      901 caatcaagtt gaataatatt aaaaagccta gtcataggct tttttggatg ttataatatg
      961 gcttgactaa gaataatagc ataaaggcag aggaatatat ggttcagggt tcaattaata
     1021 ttgaaaaata taaagatttg gcgctccaaa aacaaggcga gcatcgtaaa tttttagcaa
     1081 gtttaaaaaa gaaagctcct aaaaacttag ataaaatagt ccaacagata catcaagagg
     1141 tttttcaaga aattgattgt acagcatgtg ctaattgttg taaaactttg ggtcctctat
     1201 ttacagaagc tgatatttct aggatttcta aacatttccg gatgaagtta tcggcttttg
     1261 aagatttatt tttagaagta gatgaagatg gagataaagt atttaaagcg atgccttgtc
     1321 cgtttttagg cgatgataac ttatgtagta tatacgatgt cagacctaaa gcttgtcgtg
     1381 aatttccaca tactgatcgt aagaaaatct atcaaattaa taacttaacg ataaaaaata
     1441 ctcttacttg tccagcggca tacctttttg tggaaaaatt gaaatcacgt atagggaaat
     1501 aataacccag agaaagtatc ataaaaatat tgacaatctg cttttatttg ggtatcctag
     1561 taagtgaacg tgaaaatgtt acaatatctt gaggaggtga aacatatgtc aaaaacagta
     1621 gtacgtaaaa acgaatcact tgacgatgca cttcgtcgtt tcaaacgttc tgtgactaaa
     1681 gctggtactc ttcaagaatc acgtaaacgt gaattctacg aaaaaccttc tgtaaaacgt
     1741 aaacgtaaat cagaagcagc tcgcaaacgt aaaaaattct aattagatag aatatgaaaa
     1801 aacagttcag aattgagctg ttttttcata tattataact ataagttaat aaaaaaactc
     1861 ggtaatccga gtcttttatt atttgttagc gagtaagtcg cggatttcag caagtaattg
     1921 ctcttgtgtt ggttcaacaa cttcaataat ttcttcttca gcaggtttct tagccataac
     1981 cttgttagca gctttaacga tgaagaataa agtagtacca acgattaaga aattaataac
     2041 tgcacttaag aaattaccgt aagcaacacc attccatgaa agctgtgcga tatttgaaac
     2101 gccagcagct tttaatacag gattaaggat aagcggtgta ataacgtctt taacaagtga
     2161 agtgatgata gcattaaaag ctgcacctaa aataacagca acagcaaggt ctaaaacatt
     2221 acccttaaat aaaaattctt ttagttcttt aatcatgtga acaagctcct taatattatt
     2281 taattcacgt tcattatagc agatttcttt tgagaaatcc aagtaatagg tttcaaatta
     2341 tctgtaaatt tttatattaa atcagtgaag aagaaatcct tgaaaaagct tttgaaaaaa
     2401 tttacacaga aaagaaaaaa tgatataatc aaatgtaaaa ctataatggg ttatttctgt
     2461 ggaggtcatg acttggcaat tgataaagaa aaaatttctg aaatcaagaa cagtgttaac
     2521 attgttgatg tcatcggtga agttgtcggt ttgacaaaaa caggacgcaa tcatcttggt
     2581 ttatgcccat ttcataaaga aaaaacgcca tcttttaatg ttatcgaaga taggcagttt
     2641 tttcattgtt ttggttgtgg tcgctctggt gatgtcttta aatttgtgga agattaccaa
     2701 catattagtt ttttagatag tgtacaagtt ctagctgaac gttcaggaat acctctagat
     2761 actaatttta aggggcaagt tcctaaaaaa cctaaagcta atcagtcgct tttagatatt
     2821 catagagttg cttcgggatt ttaccatgcc tatttaatga caactaatga tggtgaacgc
     2881 gctagacaat accttgccga gcgcggagtg acagaggact taattaaaca ttttcaaata
     2941 ggattatcgc cgggagggca ggattttcta tatcgtcgtc tggcaaaaga gtttgatgaa
     3001 aaaactttga tgtcttctgg tttgttcaat tattcagaaa atagcaatca gttttacgat
     3061 tcatttaata atagaattat gtttccgtta actaatgata ttggtgaagt cattgcgttt
     3121 tcaggacgag tttggactca agaggacatt gatagaaaac aagctaaata taaaaattct
     3181 cgtgccaccc ctatttttaa taagtcttac gagctttacc atttagataa agcgcgtgct
     3241 gtaattaata aagcacacga agtttaccta atggaaggct ttatggatgt tattgcagct
     3301 tatcgtgcag gaattgagaa tgttgttgct tctatgggga ccgctttaac caatgaacat
     3361 gttagacacc ttaaaagatt tactaagaaa gttgttctga cttatgatgg tgatagagct
     3421 ggtcaaaacg caattgacaa gtctttagaa ctgctatcag atatgactgt tgatatcgtt
     3481 aggataccta ataaaatgga ccctgatgaa tttctacaag caaattcagc agaagatttt
     3541 aaacaactct tagaaaatgg gcgcatcagt aatacagagt tttatatcca ctacttaaag
     3601 cctgaaaaca ccgataattt acaatctgaa attgcttatg ttgaaaaaat agcgaaatta
     3661 attgctaaat ctccatcgat aacagcacaa aattcatata ttacaaaagt ggctgaactc
     3721 ttgccagatt ttgattattt tcaagtagaa caatcggtta ataacgaacg attgcatcat
     3781 agaagtcaac agcaagcttc atcttcagta caaacaagtg ctacagtgca attaccacag
     3841 acaggtaagt tatcagctat cactaaaaca gaaatgcaat tgtttcatcg attattgaat
     3901 catccctacc tgttaaatga attccgtaat agggataatt tttattttga tacaacagaa
     3961 atacaagttt tatatgagtt gctaaaagaa agtggtgaga tcacgtctta tgatttatca
     4021 caagaaagtg acaaagttaa tcgtacttat tatataattt tagaggaaca attacctgta
     4081 gaagtatcta ttggagagat tgaagccgtc gaaaaggcac gtgaccgttt attgaaagaa
     4141 cgtgatttaa gaaaacaaag ccagttaatt agacaatcta gtaatcaagg tgatgaagaa
     4201 ggagccttgg ctgcattaga aaatttaatt gctcaaaaaa gaaatatgga atgaggaacc
     4261 tatggcagag aaaaaaggaa atacaacttt taatgttcaa gttgcggatt ttattcgcaa
     4321 tcataaaaaa caaggtactg caattgatga tgaggtaaca gaaaaacttg ttattccttt
     4381 tgttttagat gcagatcaaa ttgatgattt gttagaacga ttgacagacg gtggcatttc
     4441 aatcacagat aaagaaggca atccttcaac aaagtatgtg gtagaagggc caaaacctga
     4501 agaattgact gatgaagagc tcataggaag taattctgct aaagtcaatg acccagtacg
     4561 tatgtacttg aaggaaattg gtgttgttcc tcttttaacg aatgaagaag aaaaagaatt
     4621 ggctgttgcg gttgctgagg gagatttaat ggctaaacaa cgtttagctg aagcaaacct
     4681 tcgtcttgtt gtctcaattg caaaacgtta tgttggacgt ggaatgcaat ttcttgattt
     4741 aattcaagag ggtaacatgg ggctcatgaa agctgttgat aaatttgatt attcaaaggg
     4801 attcaaattt tcaacttatg caacatggtg gattcgtcaa gctataacac gtgcaattgc
     4861 agatcaagca cgtacgattc gtattcctgt tcatatggtt gaaacgatta ataaattggt
     4921 ccgtgaacaa cgtaatcttc ttcaagaact tggtcaagat ccaactcctg agcaaatcgc
     4981 agaacgcatg gatatgacac cagataaagt acgtgaaatc ctaaaaattg ctcaagagcc
     5041 ggtatcgcta gaaacgccaa tcggtgagga agatgatagc catttgggtg atttcattga
     5101 agacgaagtt attgaaaatc cagtagatta tactacacgt gttgttcttc gtgagcaatt
     5161 agatgaggtt ttagatactc ttactgaccg tgaagagaat gttcttcgtt tacgctttgg
     5221 tcttgacgat ggtaaaatgc gtacgttaga ggacgtaggt aaagtattta atgttactcg
     5281 tgagcgcatt cgtcagattg aagctaaagc ccttagaaaa cttcgccatc ctagtcgtag
     5341 caagcaactt aaagatttca tggaagattg attgcttctc gataatcgtt tttagaatag
     5401 gaagtttagt ttctaataaa aatgatttgc aaaggagtta tcttaaagac ggtattaata
     5461 ttttcagaga ggtattataa tgtcagaagt aaagcaatat agcgaagaag aagttggaaa
     5521 aataaaagat cgtatcctag aagctttgga gatggttatc gatcctgaat tgggaataga
     5581 tattgttaac cttgggttaa tttatgagat tcgctttgaa gataacggtc gaactgaaat
     5641 tgatatgact ttaacgacta tgggatgccc gttagcagat ttattgacag atcaaattca
     5701 tgatgttatg aaaacagtcc cagaggtaac tgaaaccgag gtgaaattgg tatggtaccc
     5761 agcctggtct gttgataaga tgagtcgtta tgctcgtatc gctttgggaa ttcgttcata
     5821 agataaagag taaagggatg aaatattccc ttttttagtg tacaataaac gaagaattaa
     5881 aagttaaact tatttttaaa ggagcaagga atgattttaa ttacaggggc aaacgggcaa
     5941 ttaggtagtg aattacgtca ccttttagac gaacgaactc aagagtatgt tgctgttgat
     6001 gtagctgaaa tggatattac aaatgccgag atggtggaca aagtctttga agaggtgaag
     6061 ccatctttag tttaccattg tgcagcttac acagctgttg atgcggcaga agatgaagga
     6121 aaagagctag actttgcaat caatgtcact ggtacagaga atgtcgctaa ggctgctgct
     6181 aaacatgatg caacacttgt ttacatttcg acggactacg tgtttgatgg tgaaaaacca
     6241 gtggggcaag aatgggaagt agatgatctg cctgatccca aaacagagta cggtcgtaca
     6301 aaacgtatgg gtgaagagtt agttgaaaaa tatacttcaa aattctatac cattcgcaca
     6361 gcgtgggtat ttggtaacta tggtaaaaac tttgtcttta cgatgcaaaa ccttgcaaaa
     6421 acacacaaaa ctctaactgt tgttaacgac caacatggac gaccaacatg gacacgtact
     6481 ttggctgagt ttatgactta tttagctgaa aatcaaaaag attttggtta ctatcattta
     6541 tcaaatgatg ctaaagaaga tacaacatgg tatgattttg cggttgaaat cttaaaagac
     6601 acagatgtgg aggttaagcc tgttgattct agtcaattcc cagcaaaagc aaaacgtcct
     6661 ttaaattcaa ctatgagttt ggaaaaagcg aaagcaacag gatttgtgat tcctacttgg
     6721 caagatgctt taaaagagtt ctacaagcag gaagttaaaa aataagatca aaacaaagag
     6781 tagtaaggct agagaatcct tttagcttac tgctcttttt agctaacttt tctaatttat
     6841 ggtataattg tatggattgt ttagctagaa tggagaagat gatgcaagat gttttcatta
     6901 taggaagtag agggttgcca gctcgttacg gtggttttga aacttttgtt tcagaattga
     6961 ttaatcatca aaaaagtttc gacataaaat accatgttgc atgccttagt gataaagaac
     7021 atcatactca ttttaacttt gctgacgctg attgttttac tataaatcct ccccaattag
     7081 ggccagcacg tgtgattgct tatgatatta tggccattaa ttatgccctt gacttggtta
     7141 agacacatga tttaaaagag cctatttttt atattttagg aaatacaatt ggtgccttta
     7201 tttggcattt tgccaataaa atacataaag tcggtggctt attgtatgtt aatccggatg
     7261 gtttagagtg gaagcgatca aagtggtctc gtcccacaca gcgttattta aaatacgccg
     7321 aaaaatgtat gactaaaaat gcagacctaa ttatttctga taatattggt attgaaaatt
     7381 acattcaatc tacctactct aatgtgaaga caaggttcat tgcttacggt acagagatta
     7441 attctaggaa attatcgtca gatgatccac gtgtcaaaca gttgtttaaa aaatggaata
     7501 ttaagtctaa gggttactat ctaatcgttg gtcgatttgt ccctgaaaac aattatgaaa
     7561 cggctattag ggagttcatg gcttcagata ctaagcgtga tttagttatt atctgtaacc
     7621 atcaaaataa cccctacttt gaaaagttgt ccttaaagac aaaccttcaa caagataaaa
     7681 gagttaagtt tgtaggtacg ctctatgaaa aagatctgct ggattatgtt cgtcaacaag
     7741 cctttgctta tattcatggg catgaagttg gcggtactaa tccaggactg cttgaggctt
     7801 tagctaatac tgatttgaat cttgttctag atgttgattt caacaaatca gtagcaggtc
     7861 tctcaagttt ttactgggct aaaaaagagg gggatttagc taagcttatt aacgatagtg
     7921 atcaacaaca ggatttatct acttacggtg atcgagcaaa agccattatt caagaaaact
     7981 atacttggaa aaaaattgtt gaagaatatg aggatttgtt tttaaatgaa agttaatatt
     8041 ttaatggcca cctacaatgg agagaagttt ctggcgcagc aaatcgaaag tatccaaaag
     8101 caaaccttta aagagtggaa cctcctaatc agagatgatg gttcaagtga caagacatgt
     8161 gatattatta gaaactttac tgctaaagat tctcgaattc gtttcattaa tgaaaatgag
     8221 caccataatt taggagttat taaatcattt tttacgctag ttaattacga agttgcagat
     8281 ttctattttt ttagtgatca agatgatgta tggttgccag aaaagttatc tgtaagttta
     8341 gaggcagcaa agcataaagc atctgatgta ccattacttg tttatactga tttgaaagtt
     8401 gttaatcaag aattaaatat tttacaagac agcatgattc gggctcaatc gcaccatgcc
     8461 aatacgacat tattaccaga actaactgag aatactgtta ctggtgggac tatgatgata
     8521 aatcatgcat tagctgagaa gtggtttact cctaatgata tcttaatgca tgattggttt
     8581 ttagcactct tagcagcaag tttaggagaa atcatttatt tggatttacc aactcaacta
     8641 taccgtcaac atgacaataa tgttttgggg gcgaggacaa tggataagag atttaagata
     8701 cttcgtgaag gtcctaaatc tatatttaca aggtattgga aacttatcca tgactcacaa
     8761 aagcaagctt ccttaattgt tgataagtat ggtgatatta tgacagctaa tgatcttgag
     8821 ctcattaaat gttttataaa aattgacaaa cagcctttta tgacaagatt aaggtggtta
     8881 tggaaatatg gttatagcaa aaatcaattt aagcatcaag ttgtgtttaa atggttgatt
     8941 gcaacaaatt attacaataa gagataagat gatgaaaaaa attttactag tgatacctgc
     9001 ttataatgaa gaaggtagta ttgcaaagac agtccagact attgtagatt ttaaggcgtc
     9061 tcgctcacta ccttttgagc tggattatat tgttattaac gatgggtcaa ccgatggtac
     9121 tccagagtta ttagatagat tgggtttaaa tcatattgac cttgttcaaa atctaggtat
     9181 tggtggctgt gtgcagacag gatacttata cgctaatcgt aatcattacg atgttgccgt
     9241 tcaatttgat ggtgatggtc aacatgatat tagaagcatt gaagatgttg ttatgccaat
     9301 cttaaatgac gaagcagatt ttgttattgg ttctcgcttt gttgataaaa aacatcaaaa
     9361 ttttcaatcg acagcaatga ggcgtcttgg cataaattta atatctgctg ctataaaatt
     9421 aacgacggga cacaaagtct atgatacaac aagtggttat agggcagcca acgcggcgct
     9481 aatagcttat ttatcgtgtc attatccagt acaataccca gagcctgaat caactgctcg
     9541 gattttgaaa aaaggctatc gtttgaagga agtcacagct aatatgtttg aacgtgaggc
     9601 aggtacttca agtataagtt ccctaaaatc gattttttat atgacggatg ttttaacatc
     9661 aattattatt gcagggttta ttaaggagga tgataaatga gtttagcatt gcaaattgaa
     9721 atgtttattt tagccattgt cctactttat atgattataa gaatggttaa taaaaacaca
     9781 ttttctgtta aacgttcttt accttggcta ttagttggtt taattctagt ctttattagt
     9841 gtatttcctg gggtggtctc atttatcgct catcaccttg gattcgcttt aacgatgaat
     9901 ttcttgttat ttggtgctat tttatttctg ttttgtttag aattatttga tactagtagc
     9961 aataccaaaa gagatgagca aattaaacgc ctgattcaag aggtttcttt gttgaaaaaa
    10021 gagatagagg atagcacttt agatgagtaa aaaacatatt tttatttgtc tgtttttcat
    10081 gcttttttta gtaggttgcc aaaataataa aaaagtaaaa acagataaaa aagtagatat
    10141 tacagcttat tattatcata ataatacccc gcaatacagt gttcagatta atgctattca
    10201 tttacaaaca gcattggata aagtgaggac gggggagttt gagagcaaag actttacaca
    10261 ctatactttt gatagtatca agcggaagta ccaagttagt ggtaaaaaag tccttatgga
    10321 taataactat agatttggtt ctggtataaa aaatactaga caggatgcta ttgctattgt
    10381 aaggttactg cttgaaaaga ataaagatta tttaatttac atgaatggct tagaagagcc
    10441 aagtgtttta tataatccag aaaataaacg ttataagttc acaaataaca aaggaaaagc
    10501 aattggtaat attccagttg gtttaactgc ttttgaaaac agcgcagaaa ctcaagaata
    10561 tgtgttgaaa aatattcaga aaaacgaaac gatttatctt ggtaataccc gagtagataa
    10621 tcctcgagta actgttaata acaaaaaaag agatactata ggagttgaat acggtaaacg
    10681 tattacttat aggattccaa tttattcaaa gcagctgact gtcagagtta gccctaattt
    10741 tgtagtagat agtactaatt ataattaccg actttctcaa gcgcctatta ttgcggaaga
    10801 aggtaaagag ggacgtttgt atccaataga tagcagtatt agtgtcgatg gtgataaaat
    10861 tattttaaat agtagtggtg atgaaacaga gcaaaaagaa ttattacaaa atagcctgta
    10921 taaacttcac tctattgata agttagactt tgatttgaca aatgctgata ttaaagagtt
    10981 agagattaca ggacatgttg cttcaaaaag agattataat ttgtctgttg ctcaggaaaa
    11041 gaaaggcgtc atagagacta aacggagtct aatttctgtt gataatcaaa aacaagatca
    11101 agggattttt gtcgaggatt ctaaaggcta tataaagaca cctagtttga gcagttatgg
    11161 tataaatttt gccatgtatg acgacaatca aaatagatta ttgcaaggtt cagagtatat
    11221 tttaggaaga ttgaatacag ataaaaaagt atctctatat caacctaatg gctcttggat
    11281 aaatactcaa aaaacgatta agagtttaaa taaagaatat cttaaccaaa atgcttacca
    11341 tattagagga aataacatta taggtattga tggctattca aatgacatag acttgaatcc
    11401 taggttatgg gcttataact ataaaaaaca agtgaaaagt aaccaatcat tatttaaatt
    11461 acgaggtttg tctaataagt ataagtattt cttaatacag acaaaatctc caaaaggcta
    11521 cttacaaagc aataaaccat tttattttac agtaaataaa gatagtgttt ctaaagcaca
    11581 atttggtaac tataatataa atggttatat aatggacatg cagtacaaca aacaagaata
    11641 caatgcactt gcaaacactc caaaaggtca aaaaacaaag aaaactttca aacccatgac
    11701 cctagtgatt ctttttgcag tagctttatt tattttctat attacagcta taagatttgt
    11761 atttaaaaga atgtgagtta actgttagac agaggtaatt atgaaagaaa tgacggtctc
    11821 agaaataaga gaagttcaac ttgaaatgtt agcttatatt gataaagttg cgcgtgataa
    11881 taaaattgaa tattctttag gcggaggcag cctactggga gcaatgagac ataaaggatt
    11941 cattccatgg gatgacgata ttgatttgat gctagaaaga agccagtatg agcgtttgat
    12001 gaaagcatta gctgatgcta ataatagcga ttttaaattg ctacatcatt ctgttgaaaa
    12061 aaatctatgg ccatttgcta agctgtatca cacaaagtca atgtacttaa gtaaaacaga
    12121 tagaattcat ccttggacag ggatttttat tgatatcttc ccacttgacc gattaccaga
    12181 gagtgcagaa gaacgtcaaa gattctttaa aaaagttcac tctgctgctg caaatttgat
    12241 gtgtacgact taccccaatt ttgctagtgg ttcccgaaag ctttatgcaa atgcaaggct
    12301 aattttgggg ctaccccgat tcatcgttta tcacggccaa gctaaaaaac gtgctgaaat
    12361 tgttgatcaa gtgatggaaa catataataa tcaagaggtt ccttacatgg gttataccga
    12421 ttcaagatat cgcttgaaag aatattttcc aagagaaatt ttttctgagt atgaagatgt
    12481 tatgtttgaa aacattaaaa cccgaaaaat taaaaatgag catgcttatc tgaatcaatt
    12541 atatgggggt aggtatatgg aattacctcc agaaagtaag agagaaagtc atagttatta
    12601 cacttggtac tggaaagaag gttaatgatg aatattggag ttatttttgc aggtggtgtt
    12661 ggtcgtcgca tgaataccaa aggtaaacca aagcaattcc tagaagtgca tggtaaaccc
    12721 attattgtgc atacaattga tattttccaa aatacagaag ctattgatgc tgtagtagtt
    12781 gtctgtgtat cagactggct agattatatg aataatctgg tagagcgttt caaccttacg
    12841 aaagtaaaag cagttgttgc aggtggagaa actggccaaa tgtcaatttt taaggggctt
    12901 gaagccgctg aacagctggc tacagatgat gccgttgttt taattcatga cggagtacgt
    12961 ccattgatta atgaagaagt cattaatgct aatatacagt cagtaaaaga aacaggaagt
    13021 gcggtaactt cagttcgtgc taaagagaca gttgttctag tcaatgattc ttctaaaata
    13081 tctgaagttg ttgatcgtac tcgttccttc attgctaaag cgccacaaag tttttatcta
    13141 tcagatatcc tttctgttga acgtgatgcc attagcaaag gaattactga cgcaattgat
    13201 tctagtactt tgatgggtat gtacaatcgt gaactcacta ttgttgaggg accatatgaa
    13261 aatatcaaga taacaacacc agatgatttt tatatgttta aagctttgta tgatgctaga
    13321 gaaaatgaac aaatttatgg gatgtaatga tgattaaatt tagtaataaa attgtacaag
    13381 aagatttagc acttattagt gaccaaaatt atattaattg ggaaagactt gctaataaag
    13441 ccgttttaat aagtggttct aatagtatgt tagcgagtta tatggtattt ttactagcct
    13501 atctcaatga gacaagaaat taccaaacgc aaattattgc gacagctaga aacattgaaa
    13561 aagcccgtga taaattttca gatttagttg ggaaagatta tttcacctta attccttatg
    13621 atgtagaagg acgcctagag tatgatggaa aagtagatta cattatccat gcagcaagca
    13681 atgctagtcc aactgctatt ttgagtaatc ctgttagcat cataaaagct aatacaattg
    13741 gtacattgaa tttattagac tttgcaaagg aaaaaacaat tgaaaacttc ttgttcttat
    13801 caacgcgtga agtatatgga acatctatta aagaagtgat tgatgaagaa gcttatggtg
    13861 gatttgatat tttagctact agagcttgtt acccagaaag caaacgtatg gctgaaactc
    13921 tactgcaaag ttactatgat caatataagg taccctttac tattgcccgt attgcacatt
    13981 catttggtcc tggtatggag cttggtaacg atggtcgtat tatgaatgat cttttatcga
    14041 atgtcattga tggcaaagat atcgttttaa aaagtagtgg tacagcagag cgtgctttct
    14101 gttatttagc agatgccgta tcagggttat ttaccatttt attaaatggt gaagttggtc
    14161 aagcttacaa tgtagcaaat gaagatcaac ccattatgat taaggatttg gcacaaaagt
    14221 tagttgatct cttttctgat aaaaatattt cagtagtatt cgatattcct aaaactatga
    14281 gtgcaggtta tagcaaaatg gggagaacac gtctcactat ggccaaatta gaagcgttgg
    14341 gatggaagcg agaagtttct cttgaatctg gaatattaaa aacagtacaa gcatttgagg
    14401 agtaatatgg taaagacagc agttttaatg gcgacataca atggcgaaaa atttatatct
    14461 gaacaacttg attcaattcg ccaacagaca ttaaaaccag attatgtatt attgagggat
    14521 gattgttcaa cggatgaaac agtcaatgtc gtcaataact atatcgcaaa acatgggtta
    14581 gaaggctgga aaattgttaa aaacgacaaa aacttaggct ggcgtttaaa ttttcgtcaa
    14641 ttacttattg atgtgttagc ctatgaggtt gactatgtct tttttagtga tcaagatgat
    14701 atttggtatc ttgataaaaa cgaacgacag tttgccatta tgtcagataa tcctcaaatt
    14761 gaggttttga gtgcagacgt cgatattaaa acgatgtcta cagaagccag tgttccacat
    14821 tttttaactt tttcttctag tgatagaatc agtcagtatc ctaaagtata tgattatcaa
    14881 actttccgtc ccggatggac cattgctatg aagagagatt ttgcgcaagc tatcgctgat
    14941 aattggactt ttggagctaa tatttcgcac gataacctta tcgagacaac tgccagtatt
    15001 attggaaatg gttataacct taacgaaagc gtaggagttc atattcgata tggaggaaac
    15061 gcaagtggta atagcaaatt tgttttgaat tggaagtcgc ctaaaacaca acatgtcaca
    15121 ggattaaaaa catttgttga tttttacgaa atcgtgcaga aggttgtcaa caaatatcaa
    15181 aatgatgtcg ctttaaatca acgcttagcc tttgaaaaag aaagattggc agcttctaaa
    15241 ttgtcattaa ttcataatag tgcttttgtg attaaaaatt ggcgactcta taactctaat
    15301 agtggacgta tgagagattt attatttgcg tttaaaaagt aaggaataat atggtaaaaa
    15361 cagcagtttt gatggcaaca tataatggtc aaggtttcat ccacgatcag ctagattcaa
    15421 ttagaaatca aacgttgaga ccagactatg tcttaatgag ggacgatggc tctactgatg
    15481 atactgtaaa ggtggttgaa gactatataa aagagcaccg tttagatggt tggtcaataa
    15541 ctagtaatga taaaaattta ggttggcgtt tgaattttcg tcagttgctt ttagacgcta
    15601 agcgtctttc tgtagattat ttatttttta gtgatcaaga tgacacatgg tatcatcata
    15661 aaaataagat gcaagttgat attatggaag aacgccaaga tataaacctt cttagcgccg
    15721 atattgatat caaaaagtta tcagaagagg ctactattcc caataatttt aagttcaatg
    15781 gtgaggaaac aatttctaag tatccattag acttttcata tcataactat cgtcaaggtt
    15841 ggactttttg tttacgcaag tcgtttgttg atcaagtaat gactttttac agtgataatt
    15901 taattttgtc acatgataat ctttttgcag ggatatctgg tgtcattggt tcaggttaca
    15961 atttaaacag aggtgttggt gaacataagc gacatgctga gaatgcaagt gggaatatct
    16021 tgaatatgag ctcaagttac gaaaggcatt tacaagaact gtattttgtt ttgagttact
    16081 atatcatcct tgtaccattt ttagaaaaaa gtagctctag tcaattaagg gaagctaaag
    16141 cttatctgga ttttaatcag caacgttata aaaatgctca aaacaaagat tttgtagcaa
    16201 tacttaaaca aatgatgact aaaaaacggt tctatgatag cttttcaaat cgcattcgtg
    16261 acttggtgtt tttattaaaa gatataaaaa ggagttaata tgaacctcaa aaaagttcgt
    16321 ctatttgaaa agttttttct agacaagaac ttgcataaaa catattttat attaggctta
    16381 acgattacta ttgtattgag cctattaatg ccatttttca atgaacctga tggacaatac
    16441 catttagctg tgtctggtcg tatttcaaat aacattattg atacttctta ctatggagaa
    16501 tataatattg attcaggtat gagacgacaa tcgacctcct atagagatgg tactagattt
    16561 gaaaaatact atttaaataa agccaatttt gttgatggta atcatttgcc tcgtgagata
    16621 aactattcta aacataactt tgttttttgg ggtcatcttg tgccagcagt tggcttattt
    16681 ttaggcaaat ttgtgtatcc ttctatgggg gtaatgatta cagtagcaag gttatttagt
    16741 tcctttgtct caatcacctt ggttgccctc attatccggt atgtgaaaaa aggaaagtta
    16801 atctactttg ctattttcct cagtcccgta gttctaaatt catttgctag tctatcttat
    16861 gactcgactg gatacattgt atttggagca tttttatcgc ttttgataaa tcttttagtt
    16921 gataaatcgt tgactaaaaa tcgtagcatt gctcttatag gatcgattct tttagtcatt
    16981 gttgcatgta agcagaatta ctggctgttt ttattgctag taataccggt attattgcag
    17041 gttaataacc ctaaattaat gggctataat cagaagctat cacatttatt tcaaggtctt
    17101 aaatctaaaa agctattttg gctatgtcta acctttttgt cacttgctat agtttatgtt
    17161 ctgaccgcaa gacatggtgg tgtccttgct gttttaagac gttatgtaat gacatttggc
    17221 tataattatg ctggtatcaa tattttaagt aatgacatta cttcctggct tgcctctcca
    17281 tatccaagtc agaattttat tcccacttgg gtgtcatcag tatggtatct catggtcttt
    17341 ctagtatggt tttctgaaga aaaatttgtt aagtcaaaaa atatgggatt ccttttctta
    17401 gctagtttct ttgtgggaat tcttggtgtc ttttttatca tgttagaata tcgtggagcg
    17461 ccaacaagct acattgaagg agttcaagga cgttacttta ctgttacatt gcttttggta
    17521 caagtttttg catcaagtat tccagcgcgt ctaaatgagg caggaagaaa agtggtgccg
    17581 tatgcgttaa ctggcttggt aattgtatca aatgccttat tgttatttga tactgttatt
    17641 agtttaatta tgaggagaaa ctaatgaaac ttttgaaaaa tatgttttat aatacctcat
    17701 accagttatt gactttgttg ttacctttag taacagttcc ttatgtttcc cgtgtactat
    17761 ctcctcaagg tatcggtatt aatgcctata cgagctccat tgtcatgtac tttactttat
    17821 ttggtgcttt agggatatct ttgtacggta atcgtgagat agcttttgtg cagtcaaata
    17881 agtataagag aagtaagata ttttgggaat tagttgtatt aaagcttgcc tctgtatcta
    17941 tagcaacgtt attattcttt ggatttgtct tacttacaaa cgaatggcaa ttattctatt
    18001 taattcaagg tattaattta ttagcgactg caactgatat ttcgtggtat tttattggag
    18061 ttgaagactt taagataatt gtcattcgta ataccattgt taagcttatt acagttgttt
    18121 tgaccttctt agttgtgaag acgccagatg acctagcact ttatatgttt ttaattgcat
    18181 ttgcatcatt actcggaaat ttaacagtct ggcatcattt aaagcatgaa atcatcaaga
    18241 taccatttag tagattagat gtcttagtac atctaagacc aactttaatg ttatttttac
    18301 cccaaatcac catgcagatt tatctctctc ttaacaagag tatgctggga gcaatggata
    18361 gtgtggtcag tgctggatat tttgatcaat ctgataaaat tattcgtatt ttatttacca
    18421 ttgtttcagc aatcggagga gttttcttgc caaggctctc cagtctattt tctgcaggaa
    18481 aagaaaagca agctaaggct ttacttttga aacttgttga tttaagcaac gctatctcaa
    18541 tgttaatgat tgcgggagtg gtaggagttt cgtcgacatt tgctgtcttc ttttttggga
    18601 aagggtatga agctgtcggg ccgctaatgg ctgctgagtc tttgatgatc atttgcattt
    18661 cttatggtaa cgctttaggg acacagtatc tactggcttc aagacgtaca aaagcttaca
    18721 caatatctgc agttattgga cttgtggcaa atgttgtgct taatatactt ttaataccaa
    18781 ttttaggtgc tatgggagct attatctcta cagttattac tgaattcatt gtctccctat
    18841 atcaagctat ctctttaaga gatgtattta cttttaaaga attaacaaga ggaatgctgc
    18901 gctatttaat tgctgctact cttagtggag ctgtactcta ttatatcaat actcaaatgt
    18961 cagtatcatt ggtaaattat gtcattcaaa gtttagtagc agtaactatc tatgtcggta
    19021 tcgtttttat aaccaaagca ccagtcattc agttatttag agattttaga aaagagcata
    19081 gaacatgaaa tatttagcgg gtattgtcac ctttaacccc aatattgaac gcttagatca
    19141 gaacattcga gcaatttatc ctcaagtaag ccacatctat atcgtagata atggctcgaa
    19201 aaataaggaa gaaatttctc aattagtagc cgattataat gaagaggggc acctaactgt
    19261 tgattattta acagaaaata aagggattgc atatgctttg aactgtatcg gtcaatttgc
    19321 agtagcacaa gaatttgatt ggttcttgac tttagatcaa gattcggttg tcctaggtga
    19381 tttgatagat aattatgaaa attaccttca tttacctaag gtcggtatgt tatcttgttt
    19441 gtatcaagat atgaaccgtg aaaatttagt gatgcaagaa tttgactata aagaaattga
    19501 agagtgtatt acttcggctg ctttaatgaa aacgagcgtt ttcgaagaaa catctggctt
    19561 tgcagaagag atgtttattg attttgttga ctctgaaatg aattatcgtc tgtcagagat
    19621 gggatataag acttatcagg ttaattttat tggtctgtta cacgaaattg gacactcttc
    19681 acgagttaaa aaatttggcc atgtttttca tgtattgaat cattcaccat ttcgaaaata
    19741 ttacatgata cgcaatgcga tctatattat aaaaaaatat ggtaagaaga agcgatacaa
    19801 atatttagta tttatgcgaa atgaatttgt tcgagtgttg gtagcagaag agcaaaagag
    19861 caaaaaaata gttgctatga ttaaaggctt aaaagatggc ttattgatga aagtggataa
    19921 atatggaaaa taaggtaaaa acagttgctg ttttctcagg atattatcta cccttcctcg
    19981 gaggtattga acgttataca gataaaatga cagcagattt ggttaagcgt ggctaccgtg
    20041 ttgttattgt aacaactaat catggtgatt taccgattat agatgaagac aagggtagaa
    20101 aaatttaccg tttaccgacc aaaaatattg tcaagcagcg ctatcctatt attaacaaaa
    20161 atcgagaata caacacgctg atgaaatatg tttcagatga aaatattgat tttgtgattt
    20221 gtaatacacg ttttcagtta acaacgctag aagggctatc ttttgcaaag aatcaccatt
    20281 tacctagtat tgttttagac catgggtcaa gtcatttttc tgtcaataat cgttttttag
    20341 atttctttgg agctatctat gaacatcttc tcacagcgcg tgttaagcat tatcgtccag
    20401 atttttatgc ggtatccaag agaagcgttg aatggttaaa acattttaat atagaagcta
    20461 aaggagttat ttataattcg gtatctgaaa gtctaggctc tgattttgct ggtacggctt
    20521 accttgaaaa atctgctgat gatattttca tcacttatgc tggtagaatt attaaagaaa
    20581 aaggcattga attgctctta gaagcatttt cgatgtcaca gtactctgaa aatgtttatt
    20641 tacagattgc aggagacgga cctgaattag cacatttgaa agagaaatat cagtccaaac
    20701 agattaactt tttggggaaa ttaaactttg aacagactat gtctttaatg gcacagactg
    20761 atatctttgt ttacccttca atgtatccag agggattacc aacctctatt ttagaagctg
    20821 gtctactttc aagtgctatt attgcaacag atcgcggtgg aacagttgaa gttattgata
    20881 gccctgagtt aggaatcatc atggaagaaa atactcagtc actccatgaa tctcttgatt
    20941 tattagtaaa agataaggca ttaagagaga aactacaaca gaatattgct aaaagaatta
    21001 aagaacattt cacatgggaa aaaacagttg aaaagttgga ttatattatt caaaaaaatt
    21061 aacattaaaa agctagagca ttacacaatg ctctagcttt ttaatgttaa tttttaagag
    21121 ttataggagc tacgattagt tatttcatta gtttttgcaa aaagtttgca atatttttct
    21181 tcctgtattg atgaaataat tttttggatt tgattgattt ggtctgaagt attaatcaga
    21241 tcaatatatg taattaaact ctctgaagaa atctcatgtt tgataaaggg agaaagcttt
    21301 ttattggtaa taataatgtc tggattaaga ttagtaatat tggaaagatc atcagttaat
    21361 aaatagtaag ggtggaaatg aatcttttta gaagaaaatc tctgtaataa gcattctgtt
    21421 aaaatttggt tattaataaa gtctgtagtt agtacggcta tctgtatcgg aggaatatga
    21481 tttttgatga ttctttctag atgagtacaa aggagaagaa aatgttgttc tttaaacgtg
    21541 tattctccaa ttttacttaa ccaatttgta attatcggtc ggagaatagt agttaattta
    21601 tcatgtctat gatagttcca tgaaggaaat gaaattgttt cagggattaa ttgatttaat
    21661 cctaaaataa atgttcggaa aaaaaagata gcaactttta ccaactcatc atgataggag
    21721 ccagataagg gaagagcatc tttgagtggc tgtaataatt tttggaattt gggataattt
    21781 tcgaaaatag ctattacatt atcgatacgc ttctcagtcc aatttggatt agaaaaagag
    21841 ttatgggaag taaggtaagc aagaaaaata tagtctatat cgtctttttt taaattaatt
    21901 ttaaaggaat ctataataac atttttgaca caatattgta atgtatcata gataaatata
    21961 ttttgtagtt cagtgaaaag ggatgagtaa gggacaacta cattataatt aatacgtttc
    22021 catgatagag acaacaatgt atcaaagaaa ataaatgtat ttgggaagta atgagaagca
    22081 gtaaaagtag ttgagtgttc taataacaat cgtttgatga gcaatttatc catcttagac
    22141 atgggataaa cttctatacc aaattgagca tttaaaaatg cgattaaata gcgaattcga
    22201 tactcgtcac cggtaatagt atttttgaca atctgaaatc caagtttttc tagtaatgga
    22261 ctaatatgtt ttcgtatgcg ataagcagtt gcacaagata gtccaaattt ttcactaaag
    22321 taagttaaag gtaacctgcc tgaatcattc gtgatcaaaa attttaaaag ttgtaaaacg
    22381 tgtgattgat gataaatata tttgaaagct tgttctctag ttacaccatt agattggtag
    22441 actattgtac tcttttcgat attcagagag agttgactgt taaataaagt tgttaaatca
    22501 tgacaatatt tcctaataag tagttcagat aaaccaagtt gatgacatg
//
LOCUS       DATRSD010000030        21966 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRSD010000030 DATRSD010000000
VERSION     DATRSD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21966
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.030"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(324..396)
                     /locus_tag="VBD85_000922"
     tRNA            complement(324..396)
                     /locus_tag="VBD85_000922"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Lys,seq:ctt)
     gene            complement(678..1946)
                     /locus_tag="VBD85_000923"
     CDS             complement(678..1946)
                     /locus_tag="VBD85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO7133486.1"
                     /translation="MADNLALRMRPRNINEIIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDEALDFIITATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNAKDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(1939..2430)
                     /locus_tag="VBD85_000924"
     CDS             complement(1939..2430)
                     /locus_tag="VBD85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133487.1"
                     /translation="MIIRPMQDCDREAVLTLQNTGWTALTSPVYDRKWTESDLEKNLA
                     NGMSFFVAEVDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            2756..3226
                     /locus_tag="VBD85_000925"
     CDS             2756..3226
                     /locus_tag="VBD85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO7133488.1"
                     /translation="MAKFGFLSVLEEELDKHLNYDFAMDWDKKNHALEVTFVLEAQNS
                     SAIETVDDQGETSSEDIVFEDYVLFYNPAKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDANTFENGRAELKETVFYAYPRY"
     gene            3269..3577
                     /locus_tag="VBD85_000926"
     CDS             3269..3577
                     /locus_tag="VBD85_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7133489.1"
                     /translation="MKSGQKIIELRLYDERRQTITIGDYIVFYLLNDKRKKLTTEVVC
                     LHIFDDFSQLYESLDLLKCGYTLDDIETARPEEMERYYPPEQIRKYGALGIELRLLHG
                     "
     gene            3570..4028
                     /locus_tag="VBD85_000927"
     CDS             3570..4028
                     /locus_tag="VBD85_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7133490.1"
                     /translation="MDSFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     GGREDNETPLECLFREVDEELSLTLTRNHIDWVKTYRDMLKPDKLSVFMVGHISQKEY
                     DSIVLGDEGQGYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            4000..4470
                     /locus_tag="VBD85_000928"
     CDS             4000..4470
                     /locus_tag="VBD85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133491.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVKRPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSVAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(4509..5225)
                     /locus_tag="VBD85_000929"
     CDS             complement(4509..5225)
                     /locus_tag="VBD85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7133492.1"
                     /translation="MKTVKEVSILSGVSVCTLHHYDKISLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGDDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSIN
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            5349..5807
                     /locus_tag="VBD85_000930"
     CDS             5349..5807
                     /locus_tag="VBD85_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO7133493.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDRNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVGDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYPPWCSDLNRTALVTQRWQLNYFIDLSRNNEGVTT"
     gene            5804..6757
                     /locus_tag="VBD85_000931"
     CDS             5804..6757
                     /locus_tag="VBD85_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO7133494.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEVVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAFDLDVVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            6757..7497
                     /locus_tag="VBD85_000932"
     CDS             6757..7497
                     /locus_tag="VBD85_000932"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO7133495.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDT
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDLVTQKATELGAAAI
                     WAFPADWSVVKWDGKKLAKKEDKLAKIAMGAAEQSKRNRLPQVRLFEKKADFQAELNG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEAGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            7494..8054
                     /locus_tag="VBD85_000933"
     CDS             7494..8054
                     /locus_tag="VBD85_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7133496.1"
                     /translation="MIIAIQGGMAVGKTTALKIIEKVYPKIAISFEDISPVVDKINKH
                     QLDNNKLDDYCEIQQLFIQHERDRYELVRDNEIVLMDYGAEEIAFYTQYFPKVAGYSW
                     TLPSNLLLEVNRLKECFADYIIFLDADKTVLYQRQKNDFNRRRHFFKWYTHHLLPAKK
                     HYLDTFSNVIHINTNHLNAEEVAELP"
     gene            8185..9066
                     /locus_tag="VBD85_000934"
     CDS             8185..9066
                     /locus_tag="VBD85_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO7133497.1"
                     /translation="MKKHKILSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNAKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            9081..9926
                     /gene="pstC"
                     /locus_tag="VBD85_000935"
     CDS             9081..9926
                     /gene="pstC"
                     /locus_tag="VBD85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7133498.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYKP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQADLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            9928..10806
                     /gene="pstA"
                     /locus_tag="VBD85_000936"
     CDS             9928..10806
                     /gene="pstA"
                     /locus_tag="VBD85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7133499.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAVGRIIGE
                     SAALIFTAGTVAKVAHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            10799..11548
                     /gene="pstB"
                     /locus_tag="VBD85_000937"
     CDS             10799..11548
                     /gene="pstB"
                     /locus_tag="VBD85_000937"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7133500.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTCQLFSLPQDERTENYITGRFG"
     gene            11545..12201
                     /gene="phoU"
                     /locus_tag="VBD85_000938"
     CDS             11545..12201
                     /gene="phoU"
                     /locus_tag="VBD85_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7133501.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            12201..12878
                     /locus_tag="VBD85_000939"
     CDS             12201..12878
                     /locus_tag="VBD85_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133502.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFKAQGFSSSELFWEAIQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            12871..14526
                     /locus_tag="VBD85_000940"
     CDS             12871..14526
                     /locus_tag="VBD85_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7133503.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNHIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIGIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            14684..16867
                     /locus_tag="VBD85_000941"
     CDS             14684..16867
                     /locus_tag="VBD85_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7133504.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSISLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLADSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AAAQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            16906..17736
                     /locus_tag="VBD85_000942"
     CDS             16906..17736
                     /locus_tag="VBD85_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO7133505.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLAKSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPNDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            17952..18704
                     /locus_tag="VBD85_000943"
                     /pseudo
     CDS             17952..18704
                     /locus_tag="VBD85_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            18701..20035
                     /locus_tag="VBD85_000944"
     CDS             18701..20035
                     /locus_tag="VBD85_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7133506.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKALRSSEVSPTDRR
                     ILQFTNGCMVSYYLLMQFLTYFEYELAINTVIYRQFLLVAYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIANHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(20106..20288)
                     /locus_tag="VBD85_000945"
     CDS             complement(20106..20288)
                     /locus_tag="VBD85_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133507.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYSLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            20577..20750
                     /locus_tag="VBD85_000946"
     CDS             20577..20750
                     /locus_tag="VBD85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7133508.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAGHLSEKTAAANARNRM
                     GLYPGSVCRVYHV"
     gene            20878..21666
                     /locus_tag="VBD85_000947"
     CDS             20878..21666
                     /locus_tag="VBD85_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133509.1"
                     /translation="MSYQFTPDLYGLLGENGTGKTTLLNLMSHFTFADSGNIYWNGQE
                     QSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVDKKKAKQEIPRLLELVGLGDV
                     GKKKISSYSGGMKQRLGIAQALINDPEIIILDEPTVGLDPKERVKFRHILSQLSTNKI
                     IILSTHIVSDVEAVAKEIIVLKNGKFIEHGNTPQLLKTIEGKVWEITTEPDLSQIPNI
                     AIVNEKVISDSRVFRVVSDICPSDSAQLVVPTLEDFYIYHFRGE"
     gene            21672..21950
                     /locus_tag="VBD85_000948"
     CDS             21672..21950
                     /locus_tag="VBD85_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133510.1"
                     /translation="MISIFPFELKKLLQRKAALGTFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGPPYI"
BASE COUNT         6895 a         3561 c         4530 g         6980 t
ORIGIN      
        1 attatcaata attggaataa caaaatcatt tatttatcct ggaattgaaa cacaatctaa
       61 agttttactg gaaaatgaat ataaataaaa taattatcaa cataacttgt tagatatctt
      121 ttcagtgaag tgtataatta atcaaataga attattatag cgcaaagttt tgacatatag
      181 ttttgaaagg aaaaaaattt tataactatt gagtagaata ttaaacattt ttacagaatt
      241 atttagggga caaatggtgg gacaaatccc ctttcagaca agcaaaaaaa cctttaaaca
      301 aaggcttttt ttgtatagta tgatgccccc tacagggctc gaacctgtga cccatagatt
      361 aagagtctac tgctctacca actgagctaa ggaggctagg aagctgtatt ggcaccgatg
      421 atccactttt tgtattgatc ccgcacaaaa taagcaggtg ggtaactcgc acctctttaa
      481 gtggcttccg cgtgttaacg gcttgcgcac tagagatgac ttcgtttccc gaaatacaaa
      541 gaaatagtcg gtcaacactt agatgtggat tcgtatgcca cagcaatgtt tctatgtctt
      601 tatgatacca ctttttataa aattttcaag caaaattatc gatttttgaa atcgattgcg
      661 atttttaaaa ctagcaatca gtctgagagc ttatcaatgc gctctttatt tgaaccaagt
      721 gcgcgttcgt atttaccagt ttcattggct tcaaaataat tatgtcctac tagtttatct
      781 ggtaagtatt gttgtttaac ccatttttca ggataggcgt gagggtactt gtaatccttt
      841 gcattgccca aggttttact accgctgtaa tgtccatctc ttaggtgacg tggaataggt
      901 aaatttcctg aacgacgtaa atcagctaag gctgcatcca tagccaaata ggctgaattt
      961 gatttaggtg atagagctaa atccacaaca atgttagcaa taagaatacg agcttctgga
     1021 aaaccaatgc gttgggcagc ttccaatgca gtcacagtat gtatttgtgc ttcggggtta
     1081 gctaagccga tatcttcata agcaataatg gtcaaacgtc ttgctaaact aggtaaatct
     1141 ccagcttcta ctaaacgagc ggcatagtgc aggcttgcat tgacatcaga acctctaatt
     1201 gatttttgta aggctgatag gacatcataa tgcccatctc catttttatc cattgtaata
     1261 taactacgct gcagactatt ttccattgtc tctagactaa tatgacggct accatcttca
     1321 ttaggagaag tagacatgac tgctagatcc aaggaattat aagcagatcg caggtctcca
     1381 tttgtggcgg taataatgaa atctagcgcc tcatcatcaa ttgtcactaa aaaaggaaat
     1441 ccccgttctt tatcggaaat tgctagttga atggcttttt taatatcctc gttagaaaga
     1501 ggttctaact caaaaatttg taccctactt ctaatggcag gagttactga aaagaaagga
     1561 ttttcagttg ttgctccaat cataataata ttgccattct caagtaaagg taaaagaaaa
     1621 tcttgttttg tcttgtcaag tctatggatc tcgtcaagta ataaaactaa gccaccagaa
     1681 aatttagctt cttctgcaat ttcttgaaga cgttttttac tatcaacagt ggcattaaaa
     1741 gtacgaaagg catatttagt agtaccagca atagcagaag caatagaagt cttcccgata
     1801 cctggtggtc catataggat catggaagat aacatattag ctgcaaccat tcgatcaata
     1861 attttgccat taccaacgag atgttgctgc ccaatgattt cattaatatt gcgaggacgc
     1921 atacgtagag caagattgtc agccatgatt attctccaaa tcttttgcga aaattaaagc
     1981 atcaacataa tgattttcaa tgaaaaaggc tttagtgatt cgagcctctt cggtaaaccc
     2041 gtatttttga tagagggaaa tggctctgct gttattgccc atgacatgca tggcaatctt
     2101 gatataacct tgagctttag cctcagttag cagagctttc aagagagctt tacccagtcc
     2161 ttgaccttgg tagggttcag cgatgagaat gccgaaagtg gcaacatgct tgccagctgg
     2221 gaaaggatag tagggaccaa agtccaagac gccagcgatc ttatcatcga cctcagcgac
     2281 aaagaaggac atgccatttg ccagattctt ctccaagtca ctctcagtcc acttgcggtc
     2341 atagacagga gaggtaagag cagtccagcc agtattttgt aaggtcagaa ctgcctcacg
     2401 gtcgcagtcc tgcataggtc tgataatcat aacatgatac aaagggacag ttcggtttac
     2461 cgaaaatttt cgtaaagaaa tagggaacga cgaagtgtcg tcagacacta ggaagttcat
     2521 cttttcttct agaaaattag tcccgtgttc aaatcctttc tattttctat atagagtttt
     2581 tctcttggct gacgctttta acatagtatg aagtcagtca aaaacgacta gtctttgaga
     2641 ctattgtaac ataaaaataa gtaggaacta ccttttgatt aacctgagtc tgtaacgttt
     2701 ttcttctata ttcatgttat aataagaaag aataaataga aaataaggac ataaaatggc
     2761 taaatttgga tttttgtctg ttctggaaga agaattggac aagcacttaa actatgactt
     2821 tgccatggat tgggacaaga aaaatcacgc tcttgaggtg acctttgtcc tagaagcgca
     2881 aaacagctct gctattgaaa ccgtggacga ccaaggggag acgagcagcg aggacatcgt
     2941 ttttgaggac tatgtactct tttacaaccc tgccaagtct cgctttgatg ctgaggacta
     3001 tctggtgact atcccctatg agcctaagaa gggcttgtcg cgtgagtttc tggcttattt
     3061 tgcggagact ctcaacgagg ttgccactga agggctgagc gacctcatgg acttcctcac
     3121 tgatgacagt atcgaagagt tcggtctttc ttgggatgcg aatacttttg aaaatggcag
     3181 agcagagctg aaagagacag tgttttatgc atatccgagg tattgagata aagcatgaaa
     3241 tgatgttagc atcggaacca tttgaaaaat gaaatcaggt cagaaaataa ttgaactcag
     3301 gttatatgac gaacgaaggc agactattac aattggtgat tacattgtct tttatctgtt
     3361 aaatgataaa agaaagaaat tgaccacaga agttgtttgt ttacatatat ttgatgattt
     3421 ttctcaactt tatgaaagtt tagatttgct aaaatgtgga tatacattgg atgatataga
     3481 aacagccaga ccagaggaga tggaacgtta ttatccccct gaacaaatta gaaaatatgg
     3541 agctttagga atagaattga ggttattgca tggatagttt tgatgagtta tttgatttct
     3601 caggtgcaaa gatcgctctt ttctgtgaag gaaagatttt gaccagccta agagatgatt
     3661 ttccagattt gccttatgca ggcttttggg acttaccagg aggtggtcgt gaggacaatg
     3721 aaacaccttt ggaatgtctt tttcgtgaag ttgatgaaga attaagtctg accttaactc
     3781 gaaaccatat tgattgggtc aagacttata gggatatgtt gaagcctgat aaattatcag
     3841 tttttatggt tggacatatc agtcagaaag agtacgatag cattgttcta ggtgatgaag
     3901 gacaaggcta caagctcatg tccattgacg aatttctaag ccataaaaag gtcattcctc
     3961 agttgcagga gaggttgaga gattatttgg aggtggaaga tgattacttt acaaaaggcg
     4021 gaagctagtg atttagagaa gattattgcc attcagagag ctagctttaa ggcagtctat
     4081 gagaaatatc acgatcaata cgatccctat gtggaagaag ttgagcagat tcgatggaaa
     4141 ctagtgaagc gaccagattg tttctatcat tttgtccttg ttgatgagac aattgttggt
     4201 tttcttagat tggttatcaa ggatgaggag aagcgtgctt ggcttggtac ggcggctatt
     4261 cttccccaat atcaagggca gggctacggc tcagtagcga tggctttatt agagaaaacc
     4321 tatcctaagc ttacaaagtg ggatttatgt acgattgcgc aagaaaaact aatggtcagt
     4381 ttttatgaaa aatgtggtta tcattcaacc cataccgaac ctgagcaaga aggaatggat
     4441 atggtctata tgacaaagaa gatagaataa aaaaagaaaa ctagatgatt gtctagtttt
     4501 ctgatagttt attggcaata gtaagcaata gcttggctga caaactcact tgttcctgct
     4561 ccaccagctt tgttaattga ttgactaaac cggtcatctg attgatacat ttgtcctagt
     4621 ccagcaagga tttcattggt acaggtgtaa aaatgctgag agatatagtc ttgtagccgt
     4681 ttcacacaca tttgaacaga ttcatcagat acattttgtg tctttaattg cccaaattgt
     4741 accattattt ttgccatttc ttggctaatt tgtgcaaagt catctgaagc atgtttttga
     4801 gcaaattctt ggtaggctgc tgtttgtccc catttttctt ttgcttcagc ttgtaattgt
     4861 tctaattctg ttttattata aacatcgaaa ttcatataat catctccttt ttcttgtaat
     4921 cgtttcgcat gggtgataac ctgctcaagg tgcgatcttt tccattcaag caatttgatt
     4981 tgttgggcta ataagtcttg cctttcctct tttgcttgct caagcaggta tttgatatct
     5041 ttgagaggga attccaactc acgaaatagc aaaatttctt gcaggcgaat caatgcttca
     5101 tcgtcataaa gtcgatagcc tgcttcggat agggcggtag gtgggaataa actgatttta
     5161 tcatagtgat gcaaggtgca cacacttaca ccgcttaaga tactgacttc tttaactgtt
     5221 ttcatggtaa taacctcctc tcacttacat agtataaagg ataacctaag gtcagggtca
     5281 agcaaaaaat aaattatttt gaaaagtatg gttaatcata ttgatttgaa gataaaggag
     5341 ggatagttat agaaatagtt gagctactaa gggaatggta ttcttccttt gaagttaaag
     5401 aagaaaagtg gaatgaaaaa tatgaaggta tcctcataag aattgaaggc gatagaaact
     5461 ataaaaggtg tcgtttagct cataagacag ctagcaagat ggggtatttt acagtatttt
     5521 ggcagaaaga tggaaatggt aaaaatattc ctttttatga acaagatgaa gtgggagatt
     5581 tgattattgt cattaaagat ggtaggagga aaggtttgtt catcattccc aaagaagttg
     5641 cagtgagtaa gggtattctt tcatctgcta actctcaagg aaagatggct atgcgatttt
     5701 accctccttg gtgtagcgat ttaaatagaa cagctttggt gacacagcga tggcagttga
     5761 actattttat tgatttaagt agaaataatg aaggagttac tacatgaaca catggaatga
     5821 actaacggtt cacgtgaatc gtgaggcaga ggaagctgtc tcgaatttat tgattgaaac
     5881 tggcagtcag ggggttgcca ttagtgactc ggctgattat cttggacagg aggatcgttt
     5941 cggcgagctt tatcctgagg tggagcaatc tgatatgatc gctattactg cctattatcc
     6001 tgatacgctt gatattgagg tggttaaggc tgatttggct gatcgtttgg ctaattttga
     6061 aggctttggt cttgcgacgg gtagcgtcaa ccttgacagt caggaattgg tcgaagagga
     6121 ctgggctgat aactggaaga agtactatga gccagctcgc attacccatg atttgaccat
     6181 cgtgccgtca tggactgact atgaggctaa ggctggtgag aaaatcatca agctggatcc
     6241 tggtatggct tttgggactg ggactcaccc aacgactaag atgagtcttt ttgccttgga
     6301 gcaagtgctt cgtggtggtg agacggttat cgatgtgggg actggttcag gcgttttgtc
     6361 tattgctagc tctctcttgg gtgccaagga catctatgcc tttgatttgg atgttgttgc
     6421 ggtgcgcgtg gctcaggaaa atatcgatat gaaccctggt actgacaata ttcatgtggc
     6481 tgctggtgac ctgctcaaag gtgtccagca agaggcagat gtcattgttg ccaatatttt
     6541 ggcggatatt ttgatccatc tgacagacga tgcctaccgt ttggtcaagg atgagggtta
     6601 tctcatcatg tctggtatca tttctgaaaa atgggatatg gtgcgtgaga gtgctgaaaa
     6661 agctggtttc ttccttgaga ctcacatggt tcaaggtgag tggaatgcct gcgttttcaa
     6721 aaagacagat gatatttcag gagtgattgg cggctaatgc aacagtattt tgtaaatggc
     6781 aaagcagatg actttgtgac catcgaggac aaggatacca tcaagcacat gttcaatgtc
     6841 atgcgattga cagaagatga ccaagtggtc ttggtttttg atgataccat caagcgtctg
     6901 gcaaaggtgg tggacagctc tgcccatcgc ttccaaattc tagaagagct agacagcaat
     6961 gttgaaatgc ctgtccaagt gaccatcgcc tcaggctttc ccaagggaga caagctagac
     7021 cttgtgacac aaaaggcgac agaattgggt gcagcggcta tctgggcctt cccagctgac
     7081 tggtcagtcg tcaagtggga tggcaaaaaa ctggctaaaa aagaagataa gctggcaaaa
     7141 attgctatgg gcgctgcaga gcagagcaag cgtaatcgtc ttcctcaggt tcgtttattt
     7201 gagaagaagg ctgactttca agcggagctt aatggctttg ataaaatttt tatcgcctat
     7261 gaagagtctg ccaaggaggg tgaaatgtca gcgctagccc agaacttgca gactgtaaaa
     7321 gcaggtgaca agctcctctt catctttgga ccagaagggg gcatttctcc aaaagaaatc
     7381 gcagcttttg aagaggctgg agcgataaaa gtgggcctgg ggccacgcat catgcgcaca
     7441 gaaacagcac cgctctatgc tctaagtgtt attagttact cagcggagtt attatgatta
     7501 ttgctattca aggtggtatg gctgtaggga aaaccacagc tttgaaaatc attgagaaag
     7561 tttacccaaa aatcgctatt agttttgagg acatttctcc agtagttgat aagattaata
     7621 aacatcagct tgataacaat aaattagacg attattgtga aattcaacaa ctatttattc
     7681 agcatgaacg agatagatac gaacttgtta gagataatga aatagtgtta atggattatg
     7741 gagctgagga aattgctttt tatacacaat actttccaaa ggtggcgggt tatagttgga
     7801 ctttaccttc taatttgtta ctagaggtaa ataggctaaa ggaatgtttt gctgattata
     7861 ttatttttct agatgcagat aagacggtcc tataccaacg tcaaaaaaat gattttaata
     7921 gacggcgtca ttttttcaaa tggtatactc atcacctgtt gcctgctaaa aaacattatt
     7981 tagacacatt ttccaatgtt attcatatta atacaaatca tttaaatgct gaggaagtgg
     8041 ctgaattacc ataataaaaa aaaaggagtt tggataattt tccaactctt tttaatttta
     8101 cattgatttt acaaaaatcc tcaaagagat ttgatatttc ttaactataa taaaatcaaa
     8161 tcaaaaaata tttaggagtt catcatgaaa aaacacaaaa tactatctct tttggcggta
     8221 tctggtctta tgggaattgg catcctagca ggatgctcaa atgattcatc atcttcatct
     8281 aaagggacaa ttaacattgt ttctagagag gaagggtctg gaactcgtgg agctttcata
     8341 gagttatttg ggatcgagtc aaaaaataaa aagggcgaaa aagtagacca tacttctgat
     8401 gcagcaactg ttaccaattc aacttcagtt atgctaacca ctgtctctaa agacccgtca
     8461 gctattggtt attcttcact aggttcatta aatagtagcg ttaaagtgct taaaattgat
     8521 ggcaaaaatg cgactgtaaa agatattaaa tcaggaagct ataagatttc tcgtcctttc
     8581 aatattgtaa caaaagaagg taaggaaaaa gaagcaacaa aagattttat tgattatatc
     8641 ttaagcaaag atgggcaagc agtcgttgag aaaaatggct atattccact tgacaatgct
     8701 aaagcatatc aagcaaaagt gtcatctggt aaagttgtta tagcaggttc aagttcagta
     8761 acgccggtta tggaaaagat taaagaagcc tatcataaag taaatgcaaa agtagatgtt
     8821 gaaattcaac aaagtgattc ttcaactggg attacaagcg caattgatgg atcagcagat
     8881 ataggtatgg catcacgtga attggataag acagagtcat ccaaaggggt caaagctact
     8941 gttattgcaa cagatggtat tgcagtagtt gttaataaga aaaataaagt taatgatcta
     9001 tcaacaaaac aagtaaaaga catttttact ggaaagacaa cgtcttggtc tgacttatca
     9061 aagtaaggta agagtaaaca atgaaacaaa caaaagaagg tcttatgcaa gctgtgtttt
     9121 tcatcacagc ttgcgtcagt gttatatcag ctattttaat ttgcctcttt ctcttctcta
     9181 gtggtcttcc agctattacg aaaatcggct ggggaaattt tatatttggt aaagtctggc
     9241 atcctagtaa taatattttt ggcatctttc caatgattgt aggttcctta tatgttacag
     9301 cgggagcttt acttttagga ggaccgatag gtatccttac tgctgttttt atggcttatt
     9361 tctgcccaga aaatatttat aagcctttaa agtcagctat caatttaatg gcgggaatcc
     9421 cttcagttgt ttatggtttc tttggactcg ttgttattgt ccctatgata aggcaatata
     9481 ttggtggctt tggtatggga gtcttagcag cttcaatttt gctaggaatt atgattttac
     9541 caacaattgt tagcatttca gaatcttctt taagagctgt tccagagagc tattatgaag
     9601 gcggtattgc ccttggtgcc tctcatgaac gaagtgtctt ctttgctgtt ttaccagctg
     9661 ctaaacgtgg tattttagca tcggttgttt tgggaattgg acgtgctatt ggtgaaacaa
     9721 tggctgtcat catggtagcg ggtaatcaag ctgacttgcc tcaatcgctg acatcaggcg
     9781 ttagaactct aaccactaat attgtcatgg aaatgggcta ttcttctggt ttgcatcgtc
     9841 aagccttaat tggtacagct gtggtactct ttatctttat attaatgatt aatatcagtt
     9901 tttcagcatt acagaaagag gaataagatg gaaaatatta accaaagaac gtcaaaacaa
     9961 tatttagcat ctttaaaaaa agatcccctt tccttctttc tttttgctat tgtctatttg
    10021 ggagcaattc taagttttgc tacaattgca tttgttgtta tttatatttt agttaagggg
    10081 cttcctcatg tgaatactgg cttgtttgca tggacttata atactcagaa cgtttcacta
    10141 ttaccagctt tcattaacac tatctttatc atcgccttaa ctttattatt tgcagttcca
    10201 cttggtattg gtggatcaat ttatctaacg gaatatgcta gacgtgataa tccttatctc
    10261 aaaattatcc gtgttgcaac cgagacattg gcagggatac cttcaattat ttatggtttg
    10321 tttggtgcat tattttttgt aaaatacaca cacttaggct tatctttgat atcgggttct
    10381 ctgactttga gcatcatgat tttacctttg attatgagga caactgaaga agctttacta
    10441 tctgttccgg attcttatcg agaaggagcc tttgcgcttg gtgctggtaa attgagaacc
    10501 atatttaaaa tcgtattacc tagtgctatg tctggtattt ttgctggtat tattttagcg
    10561 gtaggacgta tcattggtga gtcagcagca cttattttca cagctggaac agttgcaaaa
    10621 gtagcacatt cggtatttag ttcatctcgt actttagcag tacatatgta tgctatttcg
    10681 ggtgagggct tatatgttga ccaaacttat gcgactgcgg tcatcttatt gcttttagtt
    10741 attattgtca attttgtatc tggactagta gccaaacgat taggaagtga gtcgaacagt
    10801 gagtaaatta gtaattaata atttagattt gtattatgga gaatttcacg ctctgaagga
    10861 tgttaattta gacattgaag aaaaagaaat cacagccttt attggacctt ctgggtgcgg
    10921 aaagtctact ctcttgaaaa gtattaaccg tatgaatgat ttggtcaaaa attgcaaaat
    10981 cacaggggat attactttag aaggcgagga tgtctataga caattggata ttaaccaatt
    11041 gcgtaaaaag gttggaatgg tatttcaaaa acctaatccg tttccaatga gcatttatga
    11101 taatgttgct tttggtccaa gaacgcacgg tattcattcc aaagcagagt tagatgatat
    11161 cgttgaacgt tcccttaaac aggcggctct atgggatgag gttaaagatc gtcttcacaa
    11221 atctgctctg ggtatgtctg gcggacagca gcaacgtttg tgtattgcgc gtgctcttgc
    11281 tattgagcct gatgttttac ttatggatga acccacaagt gccttggatc ctatttcaac
    11341 agcaaaaatt gaagaattgg ttatccaact gaagaagaac tacactattg ttatagtgac
    11401 acataatatg caacaagctg ttcgtatttc tgataaaaca gcattctttc tgatgggaga
    11461 agtggttgaa tataacaaaa cgtgccaact cttttcattg ccacaagatg agcgtacaga
    11521 aaactacatt acagggagat tcggatgatt cgttcacgat ttgcaagtca acttaatgac
    11581 cttaataaag aaattatttt tatgggggca ttgtgtgagg acatcattgg gaagtcctta
    11641 ggtgctctaa caaatagtaa tgacgtttac cttgacgata tttctgaaac ttatcacaag
    11701 attgaacaaa tggaacggga tattgaggag agatgtttaa aacttttact tcgtcagcaa
    11761 ccagttgcca aggatttacg ccgtatttct tctgcattaa aaatggttta tgatatgaaa
    11821 cgtattggtg ctcaagctta tgaaattgcg gaaattgtta gcttaggtca tattatccaa
    11881 ggtgcaggtt ctgaaaggga tcaacttaac agcatgtcta ataatgtcat tagtatgctg
    11941 actaagagta tagatgcttt tatttatgac aatgaagaac aagcgcatca agtaattgaa
    12001 caagatagga cagttaacca ggaatttgat acaataaaga aacagttagt gttatatttt
    12061 tcggtacagg atgttgatgg tgaatatccc atagatgttt taatgatcgc caagtaccta
    12121 gagcgtattg gtgatcatac agtaaatatt gctaaatggg tgcttttctc aataacaggt
    12181 aacctagatg gagaagcgta atgatatatt gtgttgaaga tgatgctgat attcgcgaaa
    12241 tgatgctcta tactttgcaa atggcaggtt ttaaagctca aggattctcg agttcagagc
    12301 ttttttggga agctattcag gagaaagttc cagacttgat tttacttgat attatgttgc
    12361 cgggagatga tggtttgact attttagagc gtttaaggag aaaacatcaa acggaaatga
    12421 taccagtaat catgacaact gctaaaggta gtgagtatga taaagttaaa gggttagatc
    12481 ttggagcaga tgattatctc gtcaaacctt ttgggatgat ggaaatgatt tcacgaataa
    12541 aagcagtctt gagacgtagc cgccaagtag attcaaaagc tcatattatc attggaaatt
    12601 tagagattga tccgactaat tattgggtca aaaggggaac ggaaaaaatc cacttaaccc
    12661 taaaggaatt tgagttatta gtattattct tccgtaatcc caatagagtc tttacaaggc
    12721 aagaacttct ggataaggtt tggggagaac aatttttagg agaaactaga actgtggatg
    12781 ttcacattgg gacactgcga acaaaacttg gtgaggatgg ctatttgatc gctacagtaa
    12841 ggggagttgg ataccgtttg gaggaaagac atgactaaaa aaattttccg tacaacttta
    12901 tccgctagtt tgggaattgt tttagttaca attttgatga ttatgggatt tctctataat
    12961 tacttcaatc acattcagcg agaacagttg agaacacaaa cggcactggc gtcacaagga
    13021 atttcctttg aaggtaaaga ttattttgaa aatcttaaaa cttcaaatgt gcgtattact
    13081 tgggtagata ataagggaca agtgctttat gatacccaat cagatgcaaa acatatgaaa
    13141 aatcacgcca atcgtcaaga aattaaagaa gctatcaaga gtggttacgg tgagagcacg
    13201 aggtggtcag cgacacttac tgagaagtct atctatgctg cgcaacgttt aaataatgga
    13261 acgatagttc gtttatcagt tgcgcaacaa actatttttt atcttctcct tggtatgatg
    13321 agtcccttag caattatcat cttacttgct attattttat ctgtattaat tgcacgatat
    13381 atagcgaaaa aagtatcaga gcctttaaac aatattgact tagatcatcc tttatcaaat
    13441 gatagttacg aggagataac accattatta aggcgtcttg atagccatca ggctaagatt
    13501 caacatcaaa aactgttatt acaaaagcgt caaaaagaat ttgataccat tatttctaaa
    13561 attaaggaag gaatgattct tttagatgat caagcaagaa tcgttagtat caacgcagaa
    13621 gctttaaagt tattccaaat taatgatgat tggcatgggc gctttatgat ggaagttagc
    13681 cgagatttaa ctctcaaaga cttaatcgat caaggactaa aagggaaaaa gaaagaggct
    13741 aatattggca ttgaaaataa tcattaccgc gtcttggtaa gaccaactac agataacaac
    13801 cgagtaacag gattggttgt tctactgttt gatgtgactg atcaactcca aatggaacaa
    13861 cttcaacgtg aatttactgc caatgtctct catgaattga aaaccccgct tcatgttatt
    13921 tctggttact cagaactgtt agctaatcaa atggttccaa acgaagaagt cccacaattt
    13981 gcagctaaaa ttcataaaga atctgaacga ttagttaaat tggttgagga tattatcaac
    14041 ctttctcacc ttgatgaaca agaaaaatta ccgcaagaga cagtaaatct gtatgactta
    14101 acacaaaaag ttttagaagg tttacaagct aaagcagata agaagcacat acaaatcaat
    14161 tttaatggag aagaagctat tttgcgaggg aatccagttt tattgaattc cttggtgtac
    14221 aacttgtgtg acaatgctat cacctataac catgagaaag gtcaagtaaa tgtgactttg
    14281 aaaaattcac ctgatacaat taccttagaa gttagtgata cgggactagg cattgcagag
    14341 aaggacaaaa aacgtatttt cgaacgtttc tatcgcgtcg ataaaagtcg ctcaaaaata
    14401 gttgggggga ctggtttagg tttgtctatt gtaaagagtg ctttagattt tcacaatggt
    14461 agtataaaag tggacagcca tttggggcag ggaacaacaa tgaccgtttt acttcacaaa
    14521 caataaaaac tcacaaataa aagtctagat gatattatct agactttttt agtaattcag
    14581 aaaaaatata taagtaaggg tttacaaaaa acaaataagt gctataataa aaccataata
    14641 acgcaaacgt ttgcactatt aagtgcataa aaggagattg aagatgaaaa ataatgtcaa
    14701 acagctcttt agttttgaat tttggcagaa gtttgggaag gctttgatgg ttgttattgc
    14761 tgttatgcca gcagcaggat tgatggttag tattggtaac tccattagtc tattggatcc
    14821 atcaaatgtt ttattagggc gcatagctaa tgttattgct caaattggtt ggggagttat
    14881 tggtaactta cacatcttat ttgcattggc tattggtggt agctgggcta aagaacgtgc
    14941 cggtggtgct tttgcggctg gcttatcctt tattcttatt aaccttatta cagggaactt
    15001 ttttggtgtt aaaacagata tgttagcaga cagtaaagca actgttcaaa cggtatttgg
    15061 tgcaaccatc agagtttcag attactttgt taacgttctt gggcaacctg ctcttaatat
    15121 gggtgtattt gtcggtatta tttcaggttt cgttggtgca actgccttta ataaatatta
    15181 taactatcgt aaattaccag atgctttaac ttttttcaat ggaaaacgtt ttgttccatt
    15241 tgttgttatt tatcgttcag tcattgttgc attaatttta tcagtatttt ggccggttgt
    15301 tcaatcaggt atcaatggct ttggtaaatg gattgcaagt tcacaagaca gtgctccaat
    15361 attagcacca tttgtctacg gtacgttaga gcgtttattg ctcccatttg gtttacacca
    15421 tatgttaact attccaatga actacacgca actaggtggt acatacactg tccttacagg
    15481 tgcaactaaa ggagcacagg tgttgggaca agatccactg tggttagcat gggttggtga
    15541 tttaattaac ttaaaaggct caaattcaag tcaataccat cacttattga caagtgtcac
    15601 tccagcacgt tttaaagtgg ggcaaatgat tggtgcttca ggtattttga tgggattatc
    15661 ttatgctatg tatcgtaacg ttgataaaga taaaaaattg aaatacaagt caatgtttat
    15721 ttcagcagca gcggctactt tcttgacagg tgttactgag ccaattgagt acatgttcat
    15781 gtttgcagca atgccactct accttgtata tgctgtcgtt caaggatgtg cgtttgctat
    15841 ggcagatatt gttaacctcc gcgttcattc atttggtaat atagaatttt taacacgtgt
    15901 tcctatgggc ataaaagcgg gccttggtgg tgatattttc aactttgttt gggtgacact
    15961 tctttttgca gtattgatgt actttattgc aaactttatg attaaaaaat tcaatttagc
    16021 aacagcagga cgtaatggta attatgataa cgaagaggtt gataatgctc cttcaacagc
    16081 ttctggttct gctgatgcaa attctcaagt tgtacaagtt attaatttac ttggtggtcg
    16141 tgataacatt gaagatgtag atgcatgtat gactcgtctt cgtgtcacag ttaaagatgg
    16201 aaatagcgtg ggttctgaag cagcttggaa aaaagctggc gcaatgggat tggtattaaa
    16261 aggtaatggt gttcaagcta tttacggacc taaagcagat gtccttaaat cagacattca
    16321 agatttgcta gattcaggta cagttattcc aatagttgat ttagaaacag gccaacctgt
    16381 agcagcagcc caagttacaa cttataaagg aataacagaa gaaattgttt cggtggctaa
    16441 tggtcaagta gaagcattgg atgttgttaa agaccctgtc ttttctcaaa aaatgatggg
    16501 tgatggtttt gctgtggaac caactgatgg caatatctat gttccagttt caggtacagt
    16561 tactagtgtc tttccaacaa aacatgcttt tggtttatta acagaaagtg gcctagaagt
    16621 tttagtacat atcggtctag atacagtagc tcttgatggt caaccatttg aggttaaaat
    16681 ttctagtggt caaaaagtgg tagcaggtga tcttgctgtg gtagcagatt tggaagctat
    16741 caaagcagct ggtaaagaga caagtgtgat tatcgtcttt acaaatgttt cagatataaa
    16801 aactgttaaa cttgaaaaat caggtccaca aatagctaaa acagttgtgg caaaagtcga
    16861 actgtaacaa acgataggag ctgggcaagt ctcagcttct ttctgatgaa aggatactct
    16921 atggcaaaat gcttaacgtt aaatactcat tcatggatgg aagtaaatgc tctaaagaaa
    16981 ttatttgatt tagcagagca tatcttccgt gaaaaatatg atatcatctg cctacaagag
    17041 gtgaaccaga gcatatcttc ccctcttgct aagagtagtc caaattatca tccgattgaa
    17101 ggtacaccag ctcttcatca agataacttt gccttgcaat tagtgcatta cctaaatctt
    17161 caaggtctac actatcattg gacatgggct tataatcata ttggttatag caagtaccat
    17221 gaaggggtag cgatactttc tctgaaacca ttaaagccaa atgatatttt agtttcagct
    17281 gtagacgatg agactgatta tcatactcgt cgtgctctgg tagctgagac cactctgaat
    17341 gacaaagtcg ttacagtagt tagcctccat ttatcatggt ttgagaaagg atttgcggag
    17401 gagtggaaac gactagaaac taccctactt gaggtagaaa ctccactttt actcatgggt
    17461 gactttaata atcctactgg aaatcagggt tatgaacttg ttcttaatag tccattagca
    17521 ttaaaggata gccaccaaat agccaatcat gtatttgggg accatactat tatggctgat
    17581 attgatggtt gggaagggaa taaaaaggct cttaaagttg accatatttt tactagcgaa
    17641 gacctttcaa tcagttcaag tcaagtagtt tttgaaggtg gtgaagcacc tgtcgttagt
    17701 gatcattacg gcttggaaat tacgttaaat ctttagatta atctatttaa tatattaaaa
    17761 gcatcctaaa tttgtctata aaacaagact tagggtgctt tttgaaaaat atgtcacatt
    17821 atttgactcc ttacaaagtt tggaatgcat gagaaaatat gataaaatga taataactat
    17881 tataggtgat tttaataaat attttaacca attggtttat ctattagaac aattaatacg
    17941 aggagacgaa agtgaatatt tttattttag aggataattt tattcaacag gcacgtttag
    18001 agagaataat tgaggaagtt aaaattagga gaagtataca cagtaaaatg gtaaatacct
    18061 ttgaaaaacc agttcaatta ttggaatcaa tctgtgaaaa aggtatgcat aatttatatt
    18121 tcttagacat tgaaataaaa aatgatgagc agaggggctt agaggtcgca aaacaaatta
    18181 gacaaattga tccttatgct cagattgttt ttgttacaac tcattcggaa ttaatgcctt
    18241 taacctttcg ttatcaggta tcagctttag actatatcga taaaggactt tcataggaag
    18301 catttagcag aaggattgaa gaagttttac tatatgctaa taatgcttgc agtaaaaact
    18361 tactagaaga ttctttttat tttaaatcac gctatagtca agtacaagta ccttttaacg
    18421 atatccttta tatagaaacg tcattaaggt cacatagagt tgtcctttat actgcaaaag
    18481 acagaatgga attcacagcc actttggggg atattttaaa acaagaacct aggctctttc
    18541 aatgtcatcg ctctttttta gtaaatccct tgaatatttt taaagtggat aggtcaaata
    18601 aactaatcta tttaaaaaat ggttcgaatt gtttgatttc tcgtaataaa ataaaagata
    18661 ttatggtagt aatagaggaa aatcaaagag gtagaggaat atgaattcta ctctatccat
    18721 cttagagata gctttaacga taatattaac aatatttctc ttttctaaaa tcagtggacg
    18781 tcaattaact ttaatagaga ttggaatttg tattgctctg agattaggac ttgtaatttt
    18841 atttatagca gctaatacta ttattaatct ttcatttcta aagtactggc ttctccccat
    18901 ctatatgtta agtctggctt tcttgttgct aagacctata tctaaaactt tagtagtctt
    18961 ttatggcttg tttccaattg ttctcagaaa tctttgttca cgttctttag ctttttttgt
    19021 ctttcctttg tttaatattg attttcatac aaccaaccag gggttttggg gatattttgt
    19081 tgatgtcttg tctactcttt gtgttctctt atttttaaga tggatgcaat acgattttaa
    19141 agcattgcga agtagtgaag tttcgccaac agataggaga attctccaat ttacgaatgg
    19201 gtgcatggtg agttattact tattgatgca gtttttaact tacttcgaat atgaattagc
    19261 catcaatact gttatttacc gacaattttt gcttgttgct tattggatta tatttacggg
    19321 agcaatagcc agtttagacc ggagacttcg aaattcttta caggagaggt tagctgttca
    19381 aagagaactc caattggcta atatgataga ttatagccac catattgagg gattgtataa
    19441 agaagttagg agcttccgac atgattatag taacatgtta acgaccttga aactagggat
    19501 tgataatgaa gatatatcta ttgtcaaatc agtttatcag tctatcttta aagaatctaa
    19561 tacacatttc cgacatccta agtataatat agaccggttg gttaatgtag ataacccagc
    19621 cttaaaaagt cttttagcag ccaagctttc acaagcgata gccaatcata ttatggtatc
    19681 tatagaagta ccagacagta ttaaacctca agggatggaa ctgattgatt ttattaccat
    19741 tgtatctgtt ttatgggata acgctattga ggcatcttta gagacagacg atcctaagat
    19801 agccatgtct tattttacat taaaacataa acaaattttt atcatagaaa attcaactaa
    19861 ggaaaaggca atagatacgt caacgatatt caattatgag ataagtagta aggggcggaa
    19921 tagaggaata ggtctttaca atgtcagggg agttatcaat cgttatccaa atatttctat
    19981 tgcaacaagt agccaagatt ttagtttctg ccaaactttc gaaatcatat tatagtttta
    20041 aaaagttcaa tttatctaag tttattattt tcttaatacc atcaatccaa ccaccaccag
    20101 tgatattatt tagttgttcc ggcgataatt cctgaagctg tagttccatc tcgattagtt
    20161 ttggccagtt ctcgtaagtg gtttaatgaa tattttgaac catagaactt tgcgattgaa
    20221 gcaagtgcag ttactccaca atccttagta tcaacctgac gcaccaatgc ttttttgtac
    20281 gtcaacattg tgtaattcct ttcttcgtta tcgtattaat attttgccat aaaaatatat
    20341 acttattaaa aatagcttaa acagctattg caacgcgtcc aatggtttct ataaggatat
    20401 aaaggtagat agtacttttc tgttttgagg cgattcaacg acatttcagg gaataaacgt
    20461 ttctattttt atttgaatgc tatattaaat gtgtcaagtt gcaacagaca aaaattttat
    20521 ataggagata ttcaaaataa atgtaaaatc aattgaacaa ttagaagtta tggataatga
    20581 agcattatca catgttgaag gtggccttgg tcatagatgg gtatacagta agaaccggta
    20641 aacatactta tacaagtgca gggcatcttt ctgaaaagac tgctgcagca aatgcaagaa
    20701 atcgtatggg cctttaccct ggaagcgttt gtcgtgtcta tcatgtataa ttgatttttt
    20761 atttaagcga tagggaatct tccttgtcgc ttaagtttgt atagggagag acaatactat
    20821 gttattacag ctagataata tcactaaaag ctacggaaaa aaatagtact taaccaaatt
    20881 tcctaccagt ttactcctga tttgtatgga ctcttaggag aaaatggtac tggcaaaaca
    20941 accttattaa atttgatgag ccactttaca ttcgcagata gtgggaatat ttattggaat
    21001 ggccaggagc agtcggaaga attttatcgt catattggtt ttttaccgca acattttcgc
    21061 tattatgatc aatttactgg cattgctttc ttaaattata tcgctacttt aaaaggtgtt
    21121 gataaaaaga aagctaaaca agaaattcct agattactag agcttgttgg tttaggcgat
    21181 gtaggtaaaa agaagatttc atcatattcc ggtggtatga agcaacgttt aggcattgct
    21241 caagcactta tcaatgaccc tgaaataatc attctggatg aaccaactgt tggacttgat
    21301 cctaaggaac gggttaaatt tcgccatatt ttgagtcagt tatccaccaa taaaattatc
    21361 attttatcaa cgcatattgt gtcggatgtg gaagctgttg ccaaagaaat tatagtatta
    21421 aaaaatggta aatttatcga acatggcaac actccacagt tgttgaaaac tattgaggga
    21481 aaagtgtggg aaataacgac tgaaccagat ttatctcaga tacccaatat tgctattgtt
    21541 aatgaaaaag tcatttcaga tagtcgggta tttcgtgtag tgagtgatat ttgtccgagt
    21601 gattcggcac agttggttgt tccgactcta gaagatttct acatctatca ttttagaggg
    21661 gagtaagtaa gatgatatca atttttcctt ttgaattaaa aaaactgcta caacgtaagg
    21721 cagcattggg tacttttatt atcttccttc tcgctatttt tggtctattt taccagcatt
    21781 tttttgttgg gcaaattagt ggctattctg cagataaaat acatggtcga gaagcggttg
    21841 ccattaatcg tagaatagct gaaaaatatt caggggactt atctgaagaa cgagtaacaa
    21901 atattattaa aaactatgtt aatccgccag caggaccccc ctatatttga tgtttttagt
    21961 tattat
//
LOCUS       DATRSD010000031        21950 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRSD010000031 DATRSD010000000
VERSION     DATRSD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21950
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.031"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(60..230)
                     /locus_tag="VBD85_000949"
     CDS             complement(60..230)
                     /locus_tag="VBD85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133511.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFVYSRG
                     YAPKHFLSAYDK"
     gene            complement(571..1311)
                     /locus_tag="VBD85_000950"
     CDS             complement(571..1311)
                     /locus_tag="VBD85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7133512.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(1321..2871)
                     /locus_tag="VBD85_000951"
     CDS             complement(1321..2871)
                     /locus_tag="VBD85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7133513.1"
                     /translation="MKKILLSLFTALLITSGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRTLRYIEKRFDSDNYT
                     TGANQLQV"
     gene            3007..4881
                     /locus_tag="VBD85_000952"
     CDS             3007..4881
                     /locus_tag="VBD85_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO7133514.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKARKVSCQIEDLED
                     GYRLKVFNDGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKMIERHGFILSFLLRILLPSFFIIVTLFISIRVSLFRKKVNKYGQFP
                     KDLHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQTQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFVVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHQFAISVNQSSNHFSTITENVSHASNFSVNSGGSSGGFSGGGGGGAF
                     "
     gene            4927..5766
                     /locus_tag="VBD85_000953"
     CDS             4927..5766
                     /locus_tag="VBD85_000953"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO7133515.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLIAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            5886..6641
                     /gene="mecA"
                     /locus_tag="VBD85_000954"
     CDS             5886..6641
                     /gene="mecA"
                     /locus_tag="VBD85_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO7133516.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQAEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            6643..7803
                     /locus_tag="VBD85_000955"
     CDS             6643..7803
                     /locus_tag="VBD85_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO7133517.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            7968..8738
                     /gene="sufC"
                     /locus_tag="VBD85_000956"
     CDS             7968..8738
                     /gene="sufC"
                     /locus_tag="VBD85_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO7133518.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            8775..10037
                     /gene="sufD"
                     /locus_tag="VBD85_000957"
     CDS             8775..10037
                     /gene="sufD"
                     /locus_tag="VBD85_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO7133519.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHVIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            10039..11271
                     /locus_tag="VBD85_000958"
     CDS             10039..11271
                     /locus_tag="VBD85_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7133520.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            11258..11701
                     /locus_tag="VBD85_000959"
     CDS             11258..11701
                     /locus_tag="VBD85_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO7133521.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGDQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            11801..13219
                     /gene="sufB"
                     /locus_tag="VBD85_000960"
     CDS             11801..13219
                     /gene="sufB"
                     /locus_tag="VBD85_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO7133522.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(13291..14478)
                     /locus_tag="VBD85_000961"
     CDS             complement(13291..14478)
                     /locus_tag="VBD85_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7133523.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTSLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIAILCLLAGIVLLIK
                     RSR"
     gene            complement(14631..15866)
                     /locus_tag="VBD85_000962"
     CDS             complement(14631..15866)
                     /locus_tag="VBD85_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7133524.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            16107..17762
                     /locus_tag="VBD85_000963"
     CDS             16107..17762
                     /locus_tag="VBD85_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7133525.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKK"
     gene            17881..18795
                     /locus_tag="VBD85_000964"
     CDS             17881..18795
                     /locus_tag="VBD85_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133526.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     IALLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            18805..19836
                     /locus_tag="VBD85_000965"
     CDS             18805..19836
                     /locus_tag="VBD85_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133527.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRIVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            19849..20895
                     /locus_tag="VBD85_000966"
     CDS             19849..20895
                     /locus_tag="VBD85_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133528.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTDTESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKA"
     gene            20895..21827
                     /locus_tag="VBD85_000967"
     CDS             20895..21827
                     /locus_tag="VBD85_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133529.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNNPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         6980 a         3560 c         4290 g         7120 t
ORIGIN      
        1 taattgaatg aaaatttata cttgtgaaag tgtatcggtt aagttaataa ggtcagtttt
       61 tatttatcgt aagcacttag gaagtgtttt ggagcatacc cacgagagta gacaaatccg
      121 gcatcacgtg aaggataaga cactgtgtct gccttcgcaa gacttgctga agtagctaat
      181 aatagagtga cagctgaagc tgctgttaaa acactgattt taagtttcat tataaaatcc
      241 tttcaaagat aataaactat tcttgcacag tggttagttc attttgtgca taccatatta
      301 tatcatcaaa agcccattca aacaacattt tttaatttta aacaaatata taactaatta
      361 aatatcgctt aaaattaaaa aatgagattt agtaacttat ttaatagtat acaatccaat
      421 aacgactgtc taatttgatc tatagttgag gctagtcgtg gatttcacca aagatgtagc
      481 aataaaagat cccctccagt tccattgcaa tgatgacaat gacaaaacct gtaggggtat
      541 aaatagtcta tatttaaatg tgaataaatt ttattttaag aagcgttgta gaaattcctt
      601 agttctttct tgtgttggat tttcgaaaag ttgtttagga gtaccctgct ctgcaatgat
      661 tcctttgtcc atgaaaataa cgcggtctga tacttcttta gcaaattcca tctcgtgtgt
      721 cacaattatc attgttaatc cagatttagc tagatcttgc attgttttaa ggacctcacc
      781 aaccatttct ggatccaagg ctgaagttgg ttcatcaaaa agtatagctt ctgggttaac
      841 agaaagtgct cgggcgattg cgacacgttg cttttgccca cctgataatt gttttggttt
      901 agctttccaa tattgctcag tcatgccaac agcatttaag ttttctttag caatcttttc
      961 agcttcttgg cgttcacgtt ttagaacagt agtctgtgcg acaatagcat tctctaagat
     1021 gtttaaattt tcaaagaggt taaaagattg aaagaccatc ccaagttttt cacgataatt
     1081 atttaaatca taaccttttt caagaacatt gtgaccgtga tataagattt ctcctccact
     1141 tggttcttct aagaggttaa tagaacgtag gaaagttgat tttcctgatc cagaagaacc
     1201 aatgatagaa ataacttcgc ctttgttgac cgaaagcgaa atgtctttta acacttcatt
     1261 gcttccgtaa gattttttca aatgtttaat ttctaaaata gcttgtgtca taccaacctc
     1321 ctagacttgt aattgattcg caccggttgt gtaattatct gaatcaaaac gtttttcaat
     1381 gtaacgtaag gtacgtgtta cggtgaaggt taagatgaag taaataatcg caataatggt
     1441 gaatgtttgg aaatattggt aggtttgtgt tgcaacagta ttaccagaga agtagagttc
     1501 gacaacagag ataacgttca gtacagaggt atctttgata ttgataacaa actcattacc
     1561 agtagctggg agtatgttac gtacaacttg tggtagcaca atttttcgca tggtttgtcc
     1621 gtgggtgaaa ccaagtgcag ttgcggcttc aaattgtcct ttatcaacag agaaaatacc
     1681 accacgtaca atttcgctca tataagcacc agtattgatt gaaacaataa atatagctgc
     1741 tagtgtacgg tccagcgaaa ctccgaaagc ttgtgctgtt ccgtagtaga taaccattga
     1801 ttgtacaatc ataggtgtcc cgcggaagac ctcaatatag atgttaagta accaacctaa
     1861 taatttttgt aaggcagcct ttaacttatt gtcagatttt ggtgctgtac ggaaaacacc
     1921 gatgagaagg ccgataaata atccgacgat agttcccacc atagaaatta ggagggttgt
     1981 agctgttcct ctgaggaatt gagaaccatt gttttttaaa atagttgcca tctgttcaaa
     2041 gaagtttggt tttccatttt tttctttttt aacggatggt tgttctttaa tcatcttatc
     2101 catcaaggcg atttgtttat ctctagaaat gctctcgaga acttgattga cttgattaat
     2161 acgattgtca cctttacgca taccaacaga aatatttgta tcggcatcag atgttttaaa
     2221 cccttggtgc agttcaatca tttttaattt aggattagca gtttgagcgg aagttgcatc
     2281 tggacgttct gaaacatagg cgtctacaac accagattct acagcttgtc ttaattgatt
     2341 aaagtccccc atggcaactt cttttttgac accattgatt tgatcaataa ggttgtataa
     2401 gtagactcct tgttgggcag ttactttagc atttttgaaa tcactaatat ttttagcatt
     2461 agtatatttt ccttcagcat taacaactag agtaggttct gaaatgtagt aaggctttga
     2521 aaaattaatt tcttttttac gttcttcggt aggactcatt cctgcaataa tcatatcgag
     2581 ttttccagaa gttagtgctg gaacaaggcc ttcccatttt gttttgacaa caacaacttt
     2641 tttatttaac tttttagcta actttttagc gacttgtaca tcataaccat tagcatattg
     2701 atcagttcct tcaataggaa cggcaccatt tgtgttatca ttttgtgtcc agttgaaagg
     2761 cgcataggct gcctccatac cgacacgtag atattcatct gcttgtatac ttgtcatacc
     2821 tccagaggtg ataagcaaag cagtgaataa acttagtaat atttttttca ttcaacagcc
     2881 tccgataagt tacttcctcc tattttaccg aaaaacaagg tcgatttcaa tatcattatc
     2941 ttattgaaag cgattaattg tatttttatg ttaaaatggt atacaaacaa aggaaggagg
     3001 atgataatga agaaatgttt tttggctatt tgtttagctc ttagtttttt tatggtttca
     3061 gttcaagcag atgaggtgga ctataacatt cctcattatg agggtaatct aactattcac
     3121 aatgataata gtgctgattt tacagagaag gttacttacc aatttgattc gtcctataat
     3181 ggacagtatg tcacattagg tacggcgggt aagttacctg acaattttga tattaataat
     3241 aagccacagg ttgaagtttc aattaatggt aaagcaagga aagttagttg ccagatagaa
     3301 gatttggagg atggctaccg tttgaaagtg tttaatgatg gtgaagcagg tgatactgtt
     3361 aaagtcaatg ttcagtggaa actaaaaaat gttctattta tgcataagga tgttggtgaa
     3421 cttaactgga ttcctattag cgactgggat aaaacgttag agaaagtaga tttttggata
     3481 tcaactgaca aaaaggttgc tctttctcgt ctttgggggc acttgggtta tcttaaaact
     3541 cctcctaaaa taagacaaaa taataatcgt taccatttga cagcttttaa tgtaaacaaa
     3601 cgattagaat ttcatggtta ttgggataga tcttatttta atctacctac aaacagtaaa
     3661 aataattaca agaaaaaaat tgaacatcaa gagaagatga tagagcgtca tggttttatc
     3721 ctaagtttct tgttaaggat attattacct tcattcttta ttattgtgac actattcatc
     3781 tcaattaggg tgtccctgtt tagaaaaaaa gttaataaat acgggcaatt ccctaaggat
     3841 cttcatttat atgaagcacc tgaggacctt tcaccactag agttaactca aagcatttat
     3901 agtatgagct ttaaaaattt tcaagatgag gagaagaaaa cacaccttat cagtcaagaa
     3961 caactcattc agtcaattct attagacttg attgatagaa aagtattgaa ttatgatgat
     4021 aacttgttat ctctagctaa cttagataga gcttctgatg cagaaataga ttttatagag
     4081 tttgcttttg cggattctac gagtttgaag ccagaccaac tcttttctaa ttaccaattt
     4141 agttataaag aaacactacg tgaactgaaa aagcagcaca aggcttcaga tctgcaaact
     4201 caaatgagac gccgaggaag taatgcctta tcaagaatta cgcgtctcac aaggttgatt
     4261 tctaaagata atataaactc tcttagaaga aagggaattt catcacctta tcgtaaaatg
     4321 tcttcagaag agtctaaaga attatctagg ttgaaaagat tcagttacct atcacctctt
     4381 atttcttttg ttgttataat ttatacgctt tttttaaatt attttaccta tttctgtatc
     4441 tatctcttat tgtttgttgt tatcctgttg ttgaataaaa tcatttttat gatgacaaga
     4501 aaaataagta acggttatat tgtaactgaa gatggagcaa gtcgtgtcta ccaatggact
     4561 agttttagga acatgctaag ggatatcaaa tcgtttgatc gttcagagtt agaaagtatc
     4621 gtattatgga atcgaatatt ggtttacgct actttattcg gctacgctga ccgtgttgag
     4681 aaagtactca gagtgaacca aatagatatt ccagaaagat ttgcaaacat tgatagtcat
     4741 caatttgcga tttcagtcaa tcaatctagt aatcattttt caacgataac tgaaaatgtt
     4801 agtcacgctt ctaattttag tgttaattca ggcggttctt caggtggttt ctcaggcggc
     4861 ggaggtggcg gtgccttcta attttccaaa agtatgatat aataaagaaa aatttatgga
     4921 gttattatgt taataattga attactcaaa gccctatttt taggagtcgt tgagggagtg
     4981 acagaatggt tacctgtttc tagtactggc catttaatct tggttcaaga gtttatgaag
     5041 ttgaaccaaa gcaaatcctt tgttgaaatg tttaatattg ttatacaact tggggcaatt
     5101 atggcagtta ttgtcattta ttttaaacgc cttaacccat ttcagccagg taaatctgct
     5161 agagaaattc gtttaacttg gcaattatgg ttaaaagtag tgatagcttg tattccatct
     5221 attttaattg ctttaccatt tgataattgg tttgaagctc attttaattt catgatcccg
     5281 attgcaatag ccctaatctt ttatggtttt gtcttcatat gggttgaaaa acgtaatgca
     5341 cacctcaaac cacaggtaac cgaattggca agtatgtctt ataagacagc tttcttgatt
     5401 ggatgcttcc aggttctcag tattgttccg ggaaccagtc gttctggagc tactatttta
     5461 ggagcaatta ttattggaac tagtcgttcg gtcgctgctg actttacttt cttccttgcc
     5521 atcccaacta tgtttggtta tagtggactt aaggcggtta aatacttttt agatggtaac
     5581 gtcttgagtt tagaccaatc tttaatactt ttagtagcaa gtctgatagc tttcgtagtt
     5641 agtttatatg ttattcgttt cttgacagac tatgtcaaac gacacgattt caccatcttt
     5701 ggtaagtatc gtatagtctt aggaagttta ctcatcctct actggttagt tgttcattta
     5761 ttctaaaata taacataaaa agctgagtat tcctcagctt tttagcgtgt taggctgcga
     5821 aaaattgcta aaaaatagct ttttcggtat aataagaaaa ctgaatactc gtatgaggtg
     5881 atgatatgga aatgaaacaa atcagtgaaa caacactgaa aattacaatt agtatggaag
     5941 atttagaaga tcgtggtatg gagctgaaag atttcctaat ccctcaggag aagactgagg
     6001 aatttttcta ttctgtcatg gatgaattag acttgccaga aaactttaaa aatagtggta
     6061 tgttaagttt tcgagtaaca cctaaaaaag atcgcattga tgtttttgtt acaaagtctg
     6121 aattaagtaa agatttaaat ttagaagaat tagcagattt gggtgacatt tcaaaaatgt
     6181 ctccagaaga cttttttaaa acattggaac aatcgatgtt ggaaaaaggg gatacggatg
     6241 cccatgccaa attagcagaa attgaaaata tgatggataa agcaactcaa gaagtagttg
     6301 aggaaaatgt ttctgaagaa caagctgaaa aggaagtaga aacgattgga tatgttcact
     6361 atgtctttga ttttgataat attgaagctg tagttcgatt ttcacaaacg attgattttc
     6421 caatagaagc ttctgaactc tataaaaatg gtaaaggcta tcatatgaca attttgctag
     6481 acttggaaaa ccaaccaagc tactttgcta atttgatgta tgctcgaatg cttgaacatg
     6541 ctaatgtggg cacaaaaaca cgagcttact tgaaagagca tagtattcaa ttgattcatg
     6601 atgatgctat ttcaaaatta caaatgattg agatgggata aaatgatacc ttttacaatt
     6661 gagtatattt ttgtattgat tggagcattt ttattatcaa tcattctaac tcctattatt
     6721 cgagttatat ctttgaaggt aggagcggtt gataaaccga acgctcgaag aatcaacaaa
     6781 gtaccaatgc caagtagtgg tggtttagca atatttctct cctttgttgt aacaacatta
     6841 tttttcatgc caatggcggc cagtagacac tttatagaag tctcttattt tcactacatt
     6901 ttgcctgtaa ttattggcgg tctcgtagtt acaacaacag gatttataga tgacatcttt
     6961 gaattaagac ctcgttataa aatgttggga attattattg cagctattat catatggaaa
     7021 tttacacatt ttagatttga tagttttaag attccaatag gtggtccact cttggagttt
     7081 ggtcctattt taactttctt tttgacagtt ttatggatta tttcaatcac taatgctatc
     7141 aatctgattg atggtctaga tggtctagtt agtggtgttt caatcatcag tctagcaacg
     7201 atggcagtgg tttcttattt ctttttacct aaaatagatt tttttctcac gctaactatt
     7261 gttattttaa tcgcctcaat agttggtttt ttcccttata attatcatcc agccattatc
     7321 tacctaggtg atgctggtgc cttgtttata ggatttatga ttggtgtatt atcgcttcaa
     7381 ggtttaaaaa actccacagc tgttgcagtg ataacacctg tcattatttt aggagttcct
     7441 attttggata cagctgttgc tattgtgagg cgtaaattat cagggaaaaa gatttctgaa
     7501 gctgataaga tgcacctgca tcaccgattg ctatctatgg gatttacaca tagaggagca
     7561 gttttagttg tttatggtat tgcaattatc ttttcgctta tcgctttgct tttaaatgtt
     7621 tctagtcgta ttgggggaat ttttcttcta ttggctctac tattagtaat ggaaattttt
     7681 atagaaggtt taaatatttg gggtgagaat cggacaccgc tatttaatct cttaaagttc
     7741 attggcaaca gtgattacag acaaagtgtt attgctaaat atagcgataa gcatcaaaaa
     7801 taaaagcctg aaaaggcttt tttagtttta atcatttaga tagcagtcac acctcatttt
     7861 tgctataatt atttagaata taatatcggg tgagtgccct ataatatctt ctttaaacca
     7921 tagagaaata tcacatattc tctgttaacc aagaaaagga gtgtacaatg tctgtacttg
     7981 aaatcaaaaa tcttcatgtt tctattgaag ataaagaaat tttaaaaggt cttaatttaa
     8041 cgctgaaaac aggagaaatt gctgctatca tgggtccaaa tggtactggg aagtcgacac
     8101 tttcagcagc tattatggga aatcctaatt atgaagttac agcaggtgaa attctttttg
     8161 atggtgaaga tattttagag ctagaagtgg atgaacgtgc tcgtttgggt ctatttcttg
     8221 cgatgcaata cccttcagag gttccaggga ttactaatgc tgaatttatc cgagcagcta
     8281 tgaatgcggg aaaggctgat gatgataaaa tttccatccg ccaatttatt actaagttag
     8341 atgaaaagat ggaactactt ggcatgaaag aagaaatggc ggaacgttat cttaacgaag
     8401 gtttttcagg tggtgagaag aagcgcaatg aaattttaca actcctaatg ttagagccta
     8461 agtttgccct cttggatgag attgactctg gcctggatat tgatgctctt aaagtggtat
     8521 caaagggagt caatgaaatg cgtggtgaag gttttggtgc tatgattatt acacactatc
     8581 aacgcctttt gaattacatt acaccagata aagtacacgt tatgatggac gggaaggtag
     8641 tattgtcagg aggaccagaa ttagcggttc gccttgagaa agaaggttat gctcaaatcg
     8701 ctgaagagct tggtttagag tataaagaag aagtctaatt tgtcacttca actagaagag
     8761 aaaaggagtt acaaatgtct aaagaagcta tattaaattt tttacaagcc aaaggtgagc
     8821 ctacttggtt acaagaatta cgactaaagg cttttgaaaa aatagaagaa ctagaattac
     8881 cagtaattga acgtgtaaaa tttcatagat ggaatttagg tgatggaact attttagaga
     8941 atgactatac agctaacatt ccagatttca cagagttagg aaacaatccc aagttggttc
     9001 aaattgggac ccaaactgtc cttgaacaag ttcctatgga attaatagag aaaggcgttg
     9061 tctttacaga tttctattcg gctttggaag aaattccaga agtcatagag cgctactttg
     9121 ggaaagcacg tccttttgaa gaagatagac ttgctgccta tcacacagct tacttcaaca
     9181 gtggtgcagt gctttatatt ccagataatg ttgaaattac tcaaccaatc gaaggtcttt
     9241 tttatcaaga tagtcaatct aaagtaccat ttaataagca tatccttctt attgttggaa
     9301 aaaatgcaaa agttagttac ttagagcgat ttgagtcaat tggtgatggg acagaacgaa
     9361 caagtgctaa tatttctgta gaagtaattg cccaagcagg tagccaaatt aaatttgcct
     9421 caattgatcg cctcggagaa aatgtgacaa cgtttataag tcgtcgtggt cgtcacagta
     9481 gcgatgcaac aatagattgg gcccttgggg tgatgaatga gggcaatgtt gttgctgact
     9541 ttgattctga tttaattggt gatggaagcc acgctaattt aaaagtagtt gctgcttcgt
     9601 cagggcgtca agttcaagga attgatacgc gtgttacaaa ctacggctgt aattcagtag
     9661 gacatatttt acagcatggg gttattttag agcgtgggac tcttactttt aacggtatcg
     9721 gacatgttat aaaaggtgca aagggagcag acgcccaaca agaaagccga gttctcatgt
     9781 tatcagacaa agcacgttca gatgctaatc ctatcctttt gattgatgaa aatgatgtta
     9841 cagctggtca cgcagcctca attggtcagg ttgacccaga ggatttatat tatttgatga
     9901 gccgtggctt gaaccaaaaa acagcagagc aattagttat tcgtggattt ttaggaacag
     9961 tcattgctga aataccagta aaagaagttc gcgatgaaat gattgccgtt attgatacaa
    10021 aactagaaaa acgttaatat gacattacta gatagttata aactaaagca agatttccca
    10081 attttaaatc aattagttaa tgatgagccg cttatctatt tagataatgc agcaacgaca
    10141 caaaaaccga atcaagtttt agaaactttg cgagattact accaaaatga caatgcaaat
    10201 gtccatcgtg gtgttcatac tttagcagaa cgagcgactg ctcagtacga gactgctcgt
    10261 gaaaaagcaa ggcagttttt aaatgctaaa tcaagtaaag aaattttatt cacacgtggc
    10321 accacaacag gattaaattg ggtagctaag tttgccgaaa gtattttaga acgaggtgat
    10381 gaggtactta tttccattat ggagcatcat tctaatatta tcccatggca acaagcttgc
    10441 gagagaactg gtgctaaatt ggtttatgcc tatctaaaag atggttcttt agatttagaa
    10501 gatttctata ataaattatc ctctaaaact aaatttgtta gtttggcaca tatctcaaat
    10561 gtacttggct gtattacacc agtaaaagca atagcagaac gtgtgcatca agttggggca
    10621 tacatggttg ttgatggagc tcaatcagcg ccacatatgg cgattgatgt tcaagacctt
    10681 gattgtgatt tcttcgcctt atctggccat aagatgcttg gtcctactgg tattggtgtt
    10741 ctttatggga aagaaagcat tttagataag atgccacctg tcgaatttgg tggtgaaatg
    10801 attgattttg tctatgagca tagtgcaaca tggaaggaat tgccctggaa gtttgaagct
    10861 ggaacgccta atattgctgg agcgatcgct tttggagaag ctttagatta tttaacggat
    10921 gttggtatgg atgagattca tcaatatgaa caaagtttag tttcttatgt gttgcctaaa
    10981 ttacaggcta tcgatggttt aactatttac ggacctagtg atgctgaatc tcatgtaggc
    11041 gttattgcct tcaatttaga aggtttacac ccacatgatg tggcaactgc aatggattac
    11101 gaaggagtgg ctgttcgtgc aggtcatcat tgtgcacaac cccttattaa tcatcttggt
    11161 atccattcgg cggttcgagc aagtttctat ttctataata ccaaagagga ttgcgacaaa
    11221 ttggtagacg ctatccaaaa aactaaggag tttttcaatg gcactctcta aattagataa
    11281 cctctacatg gcggtggtag ctgatcattc aaaacaccct catcatcatg gatttttaga
    11341 aggcgttgaa caagtgcagt taaacaatcc tacatgtgga gatgttattt ccttatctgt
    11401 taaatttgat gggaatatta tttcagatat tgcatttgct ggtaatggtt gtacaatttc
    11461 tacggcttca tcaagcatga tgacagatgc agttattggg aaaacaaaag aagaagcatt
    11521 gcagcttgct gatgtattct ccaaaatggt tcaaggcgat caaaatccaa aacaagagaa
    11581 acttggggat gcagagtttc tagctggggt atcaaaattt ccacaacgta tcaaatgtgc
    11641 tacgctttcg tggaatgctt taagaaaggc tattgagaga gataatcagg cagaactctg
    11701 acaaccatag ttgaaggaat tacatttaaa caacttttac tgacataagg tagtctagta
    11761 gaagaatgtc ttaaatttaa atattaaaga aaggataagt atgtccgaaa taaatgaaaa
    11821 agtagaacca cagccaatag atcttgggga atataaattt ggatttcatg acgatgtaaa
    11881 gccaatttat tctacgggaa aaggtctaaa tgaggctgtt attcgtgagt tatctgcagc
    11941 taagggtgaa cctgagtgga tgttggactt tcgtctaaaa tccttggaaa cgtttaataa
    12001 aatgccaatg cagacctggg gagcagattt atcagatatt gattttgatg atattattta
    12061 ttatcaaaaa gcatctgata aacctgcgcg tgattgggat gatgttccag aaaaaatcaa
    12121 agaaactttt gaaagaattg ggattccaga agctgaaaga gcttatcttg caggagcatc
    12181 agcacaatat gaatcagaag tggtttatca taatatgaaa gaagaatatg ataagctggg
    12241 tattgttttt acggatactg actctgcact taaagagtac ccagagctat tcaaaaaata
    12301 ttttgctaaa cttgtccctc caacagataa taaattagct gctctgaact ctgctgtatg
    12361 gtcaggtgga acatttattt atgtccctaa aggtgttaag gtggatattc cacttcaaac
    12421 ttacttccgt attaataatg aaaatactgg acaatttgaa cgtactctca ttattgtcga
    12481 tgagggagca agtgttcact atgttgaagg ttgtaccgcc ccaacttatt cttcaaatag
    12541 tttacatgca gctatagttg aaatttttgc acttgatgga gcttatatgc gctatacgac
    12601 tattcaaaat tggtccgata atgtctataa tttagtgaca aaacgtgcta ccgctaaaaa
    12661 agatgcaaca gttgagtgga tagatggaaa tctaggtgct aaaacaacaa tgaaataccc
    12721 atcggtttac cttgatggtg aaggagcacg tggcacgatg ttgtctattg cttttgcaaa
    12781 caaaggacaa caccaagata cgggtgcaaa gatgattcat aatgctcccc atactagttc
    12841 atccattgtc tctaaatcaa ttgctaaggg tggaggaaaa gttgattatc gaggtcaagt
    12901 gacatttaat aaagattcca aaaaatcagt gtcacatata gaatgtgaca ccatattgat
    12961 ggatgatatt tcaaaatcag ataccatacc gtttaatgag attcataatt cacaggttgc
    13021 tttagagcat gaagcaaagg tgtctaagat ttctgaagag caactgtact acttgatgag
    13081 tcgaggttta tctgaagctg aggcaacaga aatgattgtt atggggtttg ttgagccctt
    13141 tacgaaagaa ttaccaatgg aatatgcggt agagttaaac cgcttaattt cctatgaaat
    13201 ggaaggttca gttggttaat tgaagatttc aagagataat aaaaagcccc tatgttattg
    13261 atgaaggggg cttttatatt agcacagtta ttatctagag cgctttataa gtaatactat
    13321 cccagctaaa agacaaagga tagcgatggt gcccagaaca attaaaatag atacaaaatg
    13381 tgattgaata aattgatctg tttttgtcca ccactgttct tttgtttgag gctgttcttg
    13441 aatagatttg gttttggttt tagtaacttc ttttttaggt ttaacggctt ttaatacagg
    13501 agttttagaa gaaatatttt tagcgtcttt aaaacctttt tctacaagag cgttgacaaa
    13561 cggatgacga tagtactctc cgtcttggtc tgaccaatca ccaactccta aaaccacagt
    13621 tatcaagcga gtattctggc gtttagctgt aactaaggca ttaaaagcag cgctagggct
    13681 agaaccagtt tttaagccat ctactccttc taatccaaat ttagcgccgg gggtagagta
    13741 gttataagtt gtaaatgttt cttcataagg agttccgacc atggccttga cttcaggata
    13801 ttttgtatag ttcagtatat cagggtattt tttaaggaaa tgataggtta aaattgatag
    13861 atcacgtgca gtcgtaacgt tagtagcatt gttatcgtat tcttttgggg agtaaagtcc
    13921 attaaaagca ctcgctaccg ccccactggg gttataaaag tgagtttttg tcataccgag
    13981 tttcttggcg gtttcattga ttcgtttaat gaaggcgtca ggattgtttt gtgataagtg
    14041 gttagcaatc ataatagttg ctacattaga tgacgggaca gctgtcatag taatcagttc
    14101 acgaatagga taagcaaccc cagcatgaat attattatta ctaatttcat aaatcttact
    14161 tatggcttgg tctgtttctg ttgcagtcac tgtggtgttg aggcttgttt ttcctttagc
    14221 taagtcttca aatagtaaat ataaagtaaa cattttagac atgctagcgg gatcacgttc
    14281 taaatcagcg ttatcttccc acaaaatatg acctttattg tcaataacga tagacgcttt
    14341 aggtttatta atatctttaa catcatatcc ggcttctcgg gtaatttgca taatatcttt
    14401 atgttcttca gcttgcacag agtttacaca agtcagggat aataaaatgc agaggaaaga
    14461 tacgattaat ttaggcatag gtaactcctg aaaacttttt attaatatta taacaaaaaa
    14521 tcactaataa aaaagacatt tcattagtga tttttaattt tatatattct cctaaaccta
    14581 gctaaagaaa ctgctgtgca gcaactcctt gtgtcaaagg gagattacta ttaaagtttt
    14641 tcgttaacgt agcgcacaaa atgattccac cacactttga ggaagaaact gcgctcaata
    14701 ctatcttttg ccactaaatg aacgctaggt tcctttatga gatagccttt tccaacctta
    14761 ttaaggtcaa cgtaataagc tttccctagg ttctcttttt ttgtaatagg agcagtaagc
    14821 tcttttttaa agttaatttt taactgattt tgtttagtga tatttttctt ctgtactact
    14881 ttcaaatcgt ttttagcaac agcgatgacc gattgttctt ttccgtctct cacacttgct
    14941 tctttacctt tatatgcagc tcctttagct aatacaaggt taggttcgta ggtgtttgtg
    15001 atatagttca agagagagtt agttgctgta aagcgagcat atttgtcttt atcggcctta
    15061 tcagcatgca taataacagt gagtagtctc attccacttt cagtagatgt agcaataaaa
    15121 gattggccag ctaattccgt tgtcccagtt ttcaaacctg taatacctgg tctaaagaca
    15181 ggcatatctg gtagcatgta gttataagaa tgcataatat ctttatcaaa tttagcaacg
    15241 gacttactag taatcttaag aatggaagga tattcgttga ccaaatggta ggcaacaata
    15301 gcaatatcac gtgcactcat tttattttcg tcgttttgtg acgattttgg ataaatgtga
    15361 ttgcctaaca tactattatt taagccagaa gcattgacta ggtggctatc atgaattccc
    15421 cacttttcca attgagcagt cattttatca acaaatttac tttcggttcc tgaaatatgt
    15481 tcagccaaag caatggctgc actgttagca ctagaaatca tggcagcgtc cacgagctgt
    15541 ttaacagtat agcgcctttt ttctaaagga acattactag catcagattc gcgtgttagt
    15601 tgataagggt agtcagatat atttactttg gtattccact tgaggttacc gttatcaatt
    15661 tctttataga ccatataaac ggtcattatt ttagtcaagg aagcaatagc agcgggtttg
    15721 ttagcatctt tttcatacaa gatttttcct gaatctaaat caactgccaa ggcatgttta
    15781 gcggaagcat taaaagattc agcagagatg gttggcataa aaaaaatgca actaagtaat
    15841 agaatagaag taataatttt tttcaaaata acacctttag aaatttttta aactttgtcc
    15901 ctcctattat atcatatcac tatatcatac taggaattat tatttttgat aattaaataa
    15961 agaaaacgtt tgttataaaa atgtaaaaaa atgtaaaaaa atgaaagtta aaaaattatc
    16021 tgaaaatttt gcgtttttgt cttttcttaa gaattttttg tggtataatg ttagtcacta
    16081 gataaatgaa ttaggagaac aaagctatgg caaaacagaa aaataactgg cgccgtgttg
    16141 gagttggtgt ccttacactt gcttcagttg cgactcttgc tgcatgtgga agtaaatcag
    16201 cttcccagga ttctaatgga gcgattaatt gggctattcc aacagaaatc aatacactag
    16261 atttatctaa agtcacagac acttactcaa atctagctat tggtaactct agtagtaatt
    16321 tccttcgctt agataaagat ggaaagacaa gaccagattt ggctactaaa gtagatgttt
    16381 caaaagatgg cttaacttat acagctacat tacgtaaagg cttgaagtgg tcagatggca
    16441 gtaaacttac tgcaaaggat tttgtttatt catggcaacg tttagttgat cctaaaacag
    16501 cttcacaata tgcttacctt gctgttgaag ggcatgtgct taatgccgat aaaatcaacg
    16561 aaggacaaga gaaagacttg aataagctag gtgttaaggc ggaaggcgat gacaaagttg
    16621 ttattacttt atctagtccg tctccacaat ttatctacta ccttgcattc actaacttca
    16681 tgccacaaaa acaagaagtt gttgaaaaat atggaaaaga ttacgcaact acttcaaaaa
    16741 atacagttta ctcaggacca tatactgttg aaggttggaa tggttcgaat ggtactttca
    16801 cgctgaagaa aaacaaaaat tattgggacg ctaaaaatgt aaaaacaaaa gaagttcgca
    16861 tccagactgt taaaaaacca gataccgccg ttcaaatgta taaacgtggt gagttagatg
    16921 cagctaatat ctcaaatact tctgctattt atcaagctaa taagaataat aaagatgtca
    16981 cagatgttct agaagcgacc actgcctata tggaatataa tactactggt tctgtgaaag
    17041 ggcttgataa tgttaagatt cgtcgcgcct taaacttagc aactaaccgt aaaggagttg
    17101 ttcaagcagc cgttgataca ggctcaaaac cggcaattgc ttttgcacct actggtttag
    17161 ccaaaacacc agatggaact gatttggcaa aatatgttgc cccaggttat gaatataata
    17221 aaactgaagc agcaaaactc tttaaagaag gtttggctga atcaggcttg actaagctaa
    17281 aattaacaat tacagcagat gctgatgctc ctgctgccaa aaactctgtt gactatatca
    17341 agtctacttg ggaagctgct cttccaggac ttactgttga agaaaaattt gtaaccttta
    17401 aacaacgctt agaagacagt agaaaacaaa actttgacat cgtagtttct ctttggggtg
    17461 gggattatcc agaaggatca actttctacg gcctctttaa gtcagattca caaaataacg
    17521 atggaaaatt tgctaacaag gactatgatg ctgcttataa caaggcaatt tctgaagatg
    17581 cgatgaaacc agcagaatca gcgaaggact ataaagaagc agagaaaatt ttatttgagc
    17641 aaggtgctta taacccactc tacttccgta gtggtaaagg tttacaaaat ccaaaattaa
    17701 aaggtgttat tcgtaatact acaggtttgt caatagactt tacacatgct tataaaaaat
    17761 aattaaatta ctttacacta aacctttgca agaattggta attgccagtt cttgcttttg
    17821 gttaagtaaa gaaagtgaaa gagtttgcta tttttagcaa ttctaagaaa aggaaatgat
    17881 atgattaaat atattttaaa gcgtgttgct attttgttag taacgctatg ggttgtcata
    17941 accctatcgt tctttctgat gcagatcctt ccaggaacac cctacaataa tccaaaactc
    18001 actgaagaaa tgatcgcatt gcttaataaa cagtatggtt tagacaagcc tgtatggcaa
    18061 caatatttga cttacctttg gaatgtactt catggtgact ttggaacgag ttatcaatca
    18121 gtgaatcagc cagtatcacg tatgattagc ttgagattag gagtttcagt acatctaggt
    18181 gttcaggctc tcgtatttgg tgttttaggc ggtattttgg taggagctat ttcggcacgt
    18241 cataagaatg ataaagtcga tggtatctta agtgttattg caacgctagg gatttctatg
    18301 ccttcattta tcattggtat cttattatta gactattttg gatttaagtg gaacttgttg
    18361 cctttatcag gttgggggac tttctctcaa accattttac cttctttagc tctaggactc
    18421 cctacattag catctgtttc acgcttcttc cgtagtgaaa tgattgaaac tttaaattct
    18481 gattatgtgc aactagctcg ttcaaaaggt atgacaattc gtcaagtaac tcgaaaacat
    18541 gcttaccgta attctatgat tccaatttta actctaattg gccctttggc ggcgggtctg
    18601 ttaactggtt ctgcactaat tgagcagatt ttctcgattc caggaattgg gcagcagttt
    18661 gttacttcga ttccaacaaa agactatcct gttattatgg ggacaaccat tgtttatgca
    18721 gtaatgttga tggttgctat cttgataaca gacgttgtta ttagtatcgt agacccacgt
    18781 gttcgcttgc agtaaggaga tgttatggca gataaaaaca gaacatttaa acttgtaggt
    18841 gcaggatctt ctagcacaca agaaaaaatt gaaaagcctg ctctttcgtt tatgcaagat
    18901 gcgtggcgtc gcttgaaaaa aaacaaatta gcagtagttt cactctattt attagctctt
    18961 ttacttactt tttcgttagc ctcaaattta tttgtaactc agaaggatgc taatgggttt
    19021 gattcgaaaa aagtaactac atatcgcaac ttaccaccta aattgagttc aaaccttcct
    19081 ttttggaatg gtagcattaa gtacgctgga aatacagaat ctacagacgc ttataagtct
    19141 caaaatgtcc ctgaaaaagt taagtatgct ttaggtacag attctctagg gagaagtgtt
    19201 gctaagcgca tcatcgtagg tattcggatt tccttacttg tagctattgc agctacattc
    19261 attgatttaa ttatcggtgt tacatacggt cttgtttcag gttttgctgg aggacgcctt
    19321 gatacattaa tgcaacgtat cgtagaggta atttcatcaa ttccaaactt agttatcgtt
    19381 acaatgttag gtttggtcct tggtaacggt attacggcta ttattatctc tattgctttt
    19441 accggatgga cttctatgtc tcgtcaggta aggaatttaa ccctatcata tcgagaaaga
    19501 gagtttgtgc ttgctgcacg ttcacttggg gaaagtccaa ttaaaatagc cttcaaacat
    19561 attttaccta acatttctgg tattatcatt gtacaaatta tgatgacaat cccaagtgct
    19621 attatgtatg aagcggtact ttcagcaatc aatttaggtg ttaaaccacc gacagcttca
    19681 ttaggatcat tgatttcaga tgcacaagaa aatttacaat attatccata tcaagttatc
    19741 ttgccagctt tagcattggt aatgatttca ttggcattta ttttgcttgg tgatgggttg
    19801 cgagatgctt ttgatccaaa atctagcgat gaataaggag gtataatcat ggaaaaagaa
    19861 actattttaa gtgttaataa tcttcatgtt gacttccaca catatgctgg agaagtaaaa
    19921 gcaattcgtg atgtcaactt tgaattaaaa aaaggtgaga ctcttgcaat cgttggtgaa
    19981 tctggttcag gaaaatctgt aactaccaga actttaattg gtttaaatgc taaaaattca
    20041 gagatatcag ggaatgttca atttaagggg cgtaaccttg ttgaactatc agaagaagag
    20101 tggactaagg tacgcgggaa tgagatttct atgattttcc aagaccctat gactagtttg
    20161 gatccaacga tgaaaattgg catgcaaata gcagaaccaa tgatgattca tcaaaaaatt
    20221 tcaaaaaagg atgctttaaa attagctctt gagctaatga aagatgtagg tatcccaaat
    20281 gctgaagagc atattaatga ttacccacat cagtggtctg gaggaatgcg ccaacgtgcg
    20341 gttatagcga ttgctttagc tgctgatcct gaaattctta ttgctgatga accaacgact
    20401 gctttagatg taacaattca agcacaaatt ttgaacttaa tgaaaaaaat tcaagcagaa
    20461 cgagactcct ccattgtttt cataactcat gacttagggg tcgttgcagg gatggcagac
    20521 cgtgtagcag ttatgtatgc agggaaaatt gttgaatttg gaactgttga tgaagtcttt
    20581 tataatccac aacatccata tacttgggga ttattgaatt caatgccgac aaccgacaca
    20641 gaatcaggta gtttagagtc aattccggga acaccaccag atttgttgaa cccacctaaa
    20701 ggagatgcat ttgcagctcg taatgaattt gcattagata ttgatcatga agaagaacca
    20761 ccgtacttca aagtttcaga aacacatttc gctgctacat ggcttttaga tgaaagatca
    20821 cctaaggtgc ttcctccatt accgatccaa aaacgttggg aaaaatggaa cgagattgaa
    20881 gggaggaaag cctaatgact gaaaatcgaa aaaaattagt tgaagtcaaa aatgtttctt
    20941 tgaccttcaa taaaggaaaa gctaatgaag tgagagcaat tgataatgtt agttttgaca
    21001 tttatgaagg tgaagtgttt ggattagttg gggaatctgg atcaggaaag acaaccgttg
    21061 gacgttcaat tttaaaactt tatgatattt ctgatggaga aattaccttt aatggagaag
    21121 tgatctcaca tctgaaaggt aaagcattac atagttttcg taaagacgct cagatgattt
    21181 ttcaggatcc tcaggctagc ttaaacggcc gtatgaagat tcgtgatatc gtagcagaag
    21241 gtttagatat acataaatta gctaaatcaa aatcagatcg tgatagtaaa gttcaggccc
    21301 tattagatct tgtagggtta aataaagatc atttaacacg ttatccgcat gaattttcag
    21361 gtggacaacg acaacgtatc gggattgcac gtgctttagc agtagagcct aaatttatca
    21421 ttgctgatga accaatatct gccttagatg tttcaatcca agcacaagtt gttaatttaa
    21481 tgcaaaaatt gcaaagagaa caaggattga cttatttgtt tatcgcacat gatttgtcta
    21541 tggtaaaata catttcagat cgtatcggtg ttatgcattg ggggaaactg ttagaagttg
    21601 gaacatctga tgatgtttat aacaatccaa tccaccctta tacgaagagt ctattatcag
    21661 ctattccaga accagatccg gagagtgaac gtcaacgtgt tcaccagccg tataatccag
    21721 ctattgagca agacggacaa gaacgtcaaa tgcacgagat tacccctggt cattttgttt
    21781 tatctacacc ccaagaagct gaagaatata aaaaacaaat attataaaag atggctagcc
    21841 catcttttat ttttttacta ataaaataaa tttaggtatg gaattttaaa ctctatgtta
    21901 ttggtgttag ccaacaggaa acttttttca gaaaatgtag aaaaagtatg
//
LOCUS       DATRSD010000032        21846 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRSD010000032 DATRSD010000000
VERSION     DATRSD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21846
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.032"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            412..1374
                     /locus_tag="VBD85_000968"
     CDS             412..1374
                     /locus_tag="VBD85_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7133530.1"
                     /translation="MKKITKMVISSVLATVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKAKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTD
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGRQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            1390..2286
                     /locus_tag="VBD85_000969"
     CDS             1390..2286
                     /locus_tag="VBD85_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133531.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKTSKSLTKEG"
     gene            2291..3094
                     /locus_tag="VBD85_000970"
     CDS             2291..3094
                     /locus_tag="VBD85_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133532.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLISIDQGAITLDNQSITKDSVEKRSKDISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            3269..3553
                     /gene="groES"
                     /locus_tag="VBD85_000971"
     CDS             3269..3553
                     /gene="groES"
                     /locus_tag="VBD85_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO7133533.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDATKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            3649..5271
                     /gene="groL"
                     /locus_tag="VBD85_000972"
     CDS             3649..5271
                     /gene="groL"
                     /locus_tag="VBD85_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO7133534.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(5306..5668)
                     /locus_tag="VBD85_000973"
     CDS             complement(5306..5668)
                     /locus_tag="VBD85_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133535.1"
                     /translation="MQNRLLKVAFFITFTELIMGLIVGAYFKLSYFLAPPKEPSMGAG
                     YGMLFAMLFILISYLILLAIIQFLAYQSQSKIIAIIVLLLFFLPLLANIGWPILMIFL
                     PQVVVTIISLLSQHKTPL"
     gene            complement(5681..6418)
                     /locus_tag="VBD85_000974"
     CDS             complement(5681..6418)
                     /locus_tag="VBD85_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7133536.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYQEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            6577..7356
                     /gene="udp"
                     /locus_tag="VBD85_000975"
     CDS             6577..7356
                     /gene="udp"
                     /locus_tag="VBD85_000975"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO7133537.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            7377..8579
                     /locus_tag="VBD85_000976"
     CDS             7377..8579
                     /locus_tag="VBD85_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO7133538.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            8609..9280
                     /gene="deoC"
                     /locus_tag="VBD85_000977"
     CDS             8609..9280
                     /gene="deoC"
                     /locus_tag="VBD85_000977"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO7133539.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            9347..10558
                     /locus_tag="VBD85_000978"
     CDS             9347..10558
                     /locus_tag="VBD85_000978"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7133540.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            10728..12368
                     /locus_tag="VBD85_000979"
     CDS             10728..12368
                     /locus_tag="VBD85_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133541.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(12393..13277)
                     /locus_tag="VBD85_000980"
     CDS             complement(12393..13277)
                     /locus_tag="VBD85_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7133542.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYTQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKILNNYKKGVG"
     gene            13321..15642
                     /locus_tag="VBD85_000981"
     CDS             13321..15642
                     /locus_tag="VBD85_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7133543.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKKNAKSRPEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGKLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYRYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSITTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            15691..15843
                     /gene="rpmG"
                     /locus_tag="VBD85_000982"
     CDS             15691..15843
                     /gene="rpmG"
                     /locus_tag="VBD85_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7133544.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            15879..16052
                     /gene="secE"
                     /locus_tag="VBD85_000983"
     CDS             15879..16052
                     /gene="secE"
                     /locus_tag="VBD85_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO7133545.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            16364..18526
                     /locus_tag="VBD85_000984"
     CDS             16364..18526
                     /locus_tag="VBD85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648230.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7133546.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFAVCSGIVHADTSSGISDS
                     IPHKKQVNLGAVTLKNLISKYRGNDKAIAILLSRVDDFNRASQDTLPQLINSTEAEIN
                     NTLPQGRIIKQSIPVVRLKVERLGSGAIKAESINNIKAESINKIQAKPINTIKAESIN
                     TIKAESIHKIKPQSIKSTSATHVKVSDQELAKQSRRSQDIIKSLGFLSSDQKDILVKS
                     ISSSKDSQLILKFVTQATQLNNAESTKARHMAQNDVASIKNISLEVLEEYKEKIQRAS
                     TKSQVDELVAEAKKVVNSNKETLVNQANGKKQEIAKLENLSNDEMLRYNTAIDNVVKQ
                     YNEGKLNITDAMNALNSIKQAAQEVAQKNLQKQYAKKIERISLKGLALSKKAKEIYEK
                     HKSILPTPGYYADSVGTYLNRFRDKRTFGNRSVWTGQSGLDEAKKMLDEVKKLLKELQ
                     DLTRGTKEDKKPDVKPEAKPEAKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKD
                     LLKDDQVKDKYVDILAVQKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDK
                     KVAKPEAKPEVKPEAKPDVKPGVKPDVKPEVKPEAKPEAKPEAKPEIKPDVKPEARPE
                     AKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPATKKSVNTSGNLAVKKAIENKKY
                     SKKLPSTGEAASPLLAIVSLIVMLSAGLITIVLKHKKN"
     gene            18732..19271
                     /gene="nusG"
                     /locus_tag="VBD85_000985"
     CDS             18732..19271
                     /gene="nusG"
                     /locus_tag="VBD85_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO7133547.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            19409..20254
                     /locus_tag="VBD85_000986"
     CDS             19409..20254
                     /locus_tag="VBD85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO7133548.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKVDGIISVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPKYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYAVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVVWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKDAKSGMPELN
                     SYLMK"
     gene            complement(20297..21544)
                     /locus_tag="VBD85_000987"
     CDS             complement(20297..21544)
                     /locus_tag="VBD85_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7133549.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGFFGMMNWLPTIIQTNLNVSVKNSSLWMIATILGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKHQKYHTNLTQLDVKPTD"
BASE COUNT         7336 a         3446 c         4426 g         6638 t
ORIGIN      
        1 atttaaatta tctatctaac aagaattttc aaaaaaatca aatttaacta ttgacaatta
       61 tttttaaatg acgtaaactt ataggcaacc ataaacaagg agagaataat atgttaatca
      121 aatcgtctat tattaaacaa acaaatactt attattactt tagtttcttt agatagacgt
      181 ttgtagcctc aaggatacaa acgtcacgca aagtttgtat ctatgaagct aaagcatttt
      241 atgatgaaag cagagccgat tagatcattt caataatcta gttggctaag gcaatatttt
      301 ttcgtcctct cgtgcacaga ggaattgaga gaaataatta accaactaga tgtagggtct
      361 agttggtttt ttgttttatg gaaaatttga tggtattaca aggagacagt aatgaaaaaa
      421 ataacaaaga tggtaattag cagcgtttta gcaactgttc tgatgacagc ttgttcacaa
      481 tcttctcaaa atggtcaatc tgatgtaaaa catattggta ttttacaata tgtagaacat
      541 ccctctctaa ctgccactag aaaaggattt ataaaagaat tagctaaaga aggctataaa
      601 gatggcaaaa atatcaaaat tgagtataag aatgctcaag gggatcagtc aaatatccaa
      661 tcaatttctg aaaaactcat taaagataat aaattagtat tgggaattgc gactccggcg
      721 gctcaatcgt tgacaactgt gtcaacagaa acacctattt tgtttacagc agtgacagat
      781 ccggtatcag ccgaattagt aaaatcaatg aaaaaacctg aaggtttagc gactggtaca
      841 agtgacatgt ctccaattaa aaagcaagta tcccttttga gaaaagtaat gcctaaggct
      901 aaacgtgttg gtattatgta taccactagc gagcgtaact cagaagttca ggtaaaacaa
      961 gcaaaaaaga tcttccaaga ggcggggatt aaaacaagtg ttaaaggtat ttcttccact
     1021 aatgatgtac aagatactgc taagagttta atgtctaaaa cagatgttct ttttgtacca
     1081 acagataata ttatagccag ctcagtaacg ctgttaggga atctatcgaa agaattaaaa
     1141 gtgcctgttg tgggaggttc agcagatatg gtaccaagtg gtttgctctt ttcttatggg
     1201 gctgattatg aggctttagg tcgtcaaaca gcaagacaag ctgttaaaat cctcaaaggt
     1261 aaagacgtcg ctaaagttcc ttcagaatat cctcaaaatc taaaagtagt agttaatgaa
     1321 gatatggcaa aagagcttgg aattgatgtt tcaagtatta aaaataataa ataatcagaa
     1381 aggtattgta tgttagatat tatcttatcc ggaatttcgc aaggattact ttggtcaatt
     1441 atggcaattg gcgtgtttat cacttttcgt atcttagaca tagccgatct ctctgcagaa
     1501 ggggctttcc ctatgggggc tgcagtttgc gccttatgta tcgttaatga tattaatcca
     1561 attgtggcaa ccatagcggg catgttaggt ggtatgttgg caggactagt atcgggtttt
     1621 cttcatacta aaatgaaaat accagccctc ctcacaggta ttattacgtt gacaggactt
     1681 tactctatta atttacttgt tttaggaaga tcaaacgtct cttttgctct caaaaatact
     1741 ttggttacaa tggtaacaag attaggttta aataaacttt cagcagtttt attaattgga
     1801 attgtttgtg taggcttggt tatcttaatt ttgtatctct ttttaaatac ccaactagga
     1861 ttagctctaa gagcgacagg tgataatgag gctatgggac aagccaatag tattaaagta
     1921 gatcggatga aaatgctagg atatatgatt gggaatggcc tgattgcttt atctggtgca
     1981 cttttagcgc aaaataatgg ctatgcagat ttgaatatgg gagtgggcac aatcgttatt
     2041 ggattagctt caattatctt ggcagaagta atgataaaat acttacctct tggcaaaagg
     2101 ttatggtcaa ttgtattagg ttctgtttta taccgtatga ttattgtttt catattaaca
     2161 accgatattg atgcacagat gataaagttg gtttcagcaa ttttgttggc tctcattttg
     2221 tatgtcccag aattacgagc taaactagga gtaaaaacct ctaaatcgct tacaaaggaa
     2281 ggataagaac atgtcattac ttgaattagt caatctgcac aaaacttttg aaaaaggaac
     2341 tgtcaatgag aaccatgttt tacgtggttt agatttaacg attgaagacg gtgattttat
     2401 ttcagtaatt ggaggtaacg gtgcaggaaa atcaaccctc cttaactgta ttgctggtct
     2461 aatctctatt gatcaggggg caatcacatt agacaaccaa tcaattacta aagactctgt
     2521 agaaaaacgg tctaaggata ttagtcgagt ctttcaggat cctagaatgg gaactgcaac
     2581 aaatttgacg attgaagaaa atatggcaat tgcacataaa agaggcaata agcgtcacat
     2641 ttttagacag tcagtgactg acgatgaccg tcaactattt aaaaagtctc tatcacaatt
     2701 aggtctaggc ttagaaaatc gaatgaagac tgatgctgca tttttatctg gaggtcagcg
     2761 tcaagccctc acgttagcta tggcaactct tgttcgtccc aaattattat tactggatga
     2821 acatacagcg gctctagatc ccaaaactag cgatatggtt atggaattaa cacagaaagt
     2881 gatagaagaa caacgattga cagccttaat gattacacac aatatggaac atgccattgc
     2941 ttatggtaat cgtctcgtga tgctctatca cgggaaaatt gttgttgatg ttaaaggaga
     3001 agcaaagaga aacttaacag ttgcagaatt aatggaactt ttccataaaa actcaggtca
     3061 acagttgata gatgatgccc ttgtcttagg ttaaaatata aatttctcta gtgaatggac
     3121 aggataacta gggaaatttt tcttgtttga aacgtgcctt ttctcttgac taattctgac
     3181 caagtgatag aatagttttt gtagttagca ctctctgtat aggagtgcta aaaaatcaaa
     3241 atatatctcg tatggaggtt cgtttattat gttaaaacca ttaggtgatc gtgtgatcat
     3301 ttcgtttgtt gaaactgaag agaaatctgt cggtggtttt gttcttgcag gagcaagcca
     3361 tgatgcgaca aagacagcca aagttcttgc tgtgggtgac ggtattcgta ccttaacagg
     3421 ggaattagtt gcacctagcg ttgcagaggg tgatactgtt ttagtcgaaa atggtgctgg
     3481 tttggaagtt aaagacggta acgaaaaagt gacagttgtt cgtgaaagtg acattgttgc
     3541 tgtcgttaaa taggttttat taagcaagaa aggatatagg cctattagag aaaagttgct
     3601 ttgtaaagaa agtagacttt gttaaatttt aggcctgggt attaaattat ggcaaaagat
     3661 attaaatttt cagcagatgc ccgctcagca atggtgcgtg gtgttgatat tttagctgat
     3721 acagtcaaag taacattagg tcctaaaggc cgtaatgttg ttcttgaaaa agcatttggt
     3781 tcgcctttaa ttacaaatga tggtgtgaca attgctaaag aaattgagct agaagatcac
     3841 tttgaaaata tgggagctaa acttgtgtca gaagtggctt caaaaactaa tgatattgca
     3901 ggggatggca ctacaactgc tactgttttg acccaagcta ttgtacggga aggtcttaaa
     3961 aatgtaactg caggggcgaa tccgattggt attcgtcgtg gtattgaaac agctgtttca
     4021 gcagcagttg aagagctaaa agagattgca caaccagttt caggcaaaga agctattgct
     4081 caagttgcag ctgtgtcttc acgttctgaa aaagttgggg aatatatttc tgaagctatg
     4141 gagcgcgtgg gtaatgatgg tgttatcact attgaagaat cgcgaggtat ggaaacagag
     4201 cttgaagttg tagaaggaat gcagtttgac cgtgggtact tgtcacagta tatggtaact
     4261 gataacgaga aaatggtctc tgaacttgag aatccgtata tccttattac agataagaaa
     4321 atttcaaata tccaagaaat tttaccatta ttagaagagg ttcttaaaac aaatcgtcca
     4381 ttgctaatca tcgctgatga tgttgatgga gaagctctcc caacgcttgt tcttaataaa
     4441 attcgtggaa ctttcaatgt cgtagctgtt aaagcgcctg gatttggtga tcgtcgtaaa
     4501 gccatgctgg aagatattgc tatcctaaca ggaggaactg tcgttactga agaccttggt
     4561 ttagacttaa aagatgctac tatgcaagtt ttaggacagt ctgctaaagt aacagtagat
     4621 aaagattcta ctgttattgt cgaaggtgcc ggtgactcat cagcaattgc taatcgcgta
     4681 gctatcatta agtcacagat ggaggctaca acttctgatt ttgatcgtga aaaattacaa
     4741 gaacgacttg ccaagttagc cggtggtgta gcagtaatta aagttggtgc agcgactgaa
     4801 acagaattaa aagagatgaa acttcgcatc gaagatgcgt taaatgcaac gcgtgctgca
     4861 gttgaagaag gtattgtttc aggtggaggt acggctcttg tgaacgttat tgaaaaagta
     4921 gcggcactga aacttaatgg tgatgaggag actggacgta atattgttct tcgtgctctc
     4981 gaagagcctg ttcgtcaaat tgcttacaat gctggatatg aaggttcagt tattattgaa
     5041 cgtttaaaac agtctgaaat tggtacagga tttaatgcgg ctaatggaga atgggtagat
     5101 atggttacca caggtatcat tgaccctgtc aaagtaacac gttctgcact tcaaaatgcg
     5161 gcatctgtag caagtcttat cttgactaca gaagcagtag tagcaaataa acctgaacca
     5221 gaagctccta cagctcctgc aatggatcca tctatgatgg gtggcttcta atcactaact
     5281 tatcacaaca taaaactccc ctttgttaaa ggggagtttt atgttgtgat aatagagata
     5341 tgatagtcac taccacttgc ggtaagaaga tcataaggat aggccatcca atatttgcca
     5401 gtagtgggag gaaaaaaagt agcaaaacaa tgattgctat tatcttagat tgagattgat
     5461 aagctaagaa ttgaattatg gctaatagaa ttaagtaact aataagtata aataacatag
     5521 caaagagcat tccgtaccca gcacccattg acggttcctt tggaggggct agaaaataac
     5581 ttaacttaaa ataagcgcca actattaggc ccataattaa ttcggtaaaa gttatgaaaa
     5641 aagcaacttt taatagtcta ttttgcatag gcttgtctcc ttaatgagag gtaatggaca
     5701 attcaaagtg ctcaatggga acgtaatgct tattacacat tagtattttc tcttggttaa
     5761 aaattgtttc ttgtattaat atgaattgtc catgatcgga tagggtatat ttggttgctg
     5821 aaaaattgcc agatgtcgca tatcccaaat ggctttgtga gtggcttgat atgccttctt
     5881 gatagatacc ttcttcaatg aagtttaaca actgtttagt atcatgtaaa gaaatctcag
     5941 catctgaaat aagttcgata gggataaaag aaagtgtgta agctaatggt ccatcatcgg
     6001 tgtggtacca acgatcagca ataacaacta caggggtttc ttgtttaagt gaagctgtga
     6061 tagcttctgc aggaacctct acacgaaatt ctaattccac ttctgtaatt ttggaagtta
     6121 gacaagtttt aatagggtgt tgtctatttt cgtaaccgtt ttcagacaag ttgctagaat
     6181 tttctctaat gaaattacct tttcctctga tatttttaac caaatggtct tcttgtaata
     6241 gtgctaatga ctttcgtaaa gtagctcgac taacattaag ttgccctgct aattcgggct
     6301 cagaaggcaa ttgcattcca ggagaataag taccgtcttg gatcatgtta aaaatcttat
     6361 tgtagacttg cacatgcttt agtttattta gtttattatt tagttcattt ttaggcattg
     6421 taacctccgt atacaagtta gtttaattga aaaaattaga aaaatcaagt taaaaaataa
     6481 aacgttttct ttcgataaaa gcgtttacaa aggttgtctt gtatgttatt atacaagtgt
     6541 aaaaaaaata gacaagacat taaggaggtc tttatcatgc agaattattc aggtgaagtc
     6601 ggattgcaat atcacctaca gattcgtcca ggtgatgtag gtcgttatgt tatcatgcca
     6661 ggtgatccaa aacgttgtgc aaaaattgca gaacattttg ataatgcagt tcttgtagca
     6721 gacagccgtg aatatgttac ttatacaggt acattaaacg gtgaaaaagt cagtgttact
     6781 tcaactggta ttggtggtcc atcagcgtct attgcgatgg aagaattgaa actctgtggc
     6841 gctgatacct ttatccgtgt tggaacatgt ggtggtattg accttgatgt taaaggcgga
     6901 gacatcgtta ttgcaactgg agctatccgt atggaaggta caagtaaaga atatgctccg
     6961 attgaatttc ctgctgtagc tgacttggag gtaacgaatg ctctagttaa tgcagctaaa
     7021 aagcttggtt acacaagtca cgcaggagta gttcaatgta aagatgcatt ttatggacaa
     7081 catgagccag aacgtatgcc agttagttat gaattattaa ataaatggga agcatggaaa
     7141 cgtctgggaa caaaagcgtc tgaaatggaa tctgcagctc tttttgtagc agcaagtcat
     7201 ctaggagttc gttgtggatc agatttcctt gttgttggta atcaagaacg caacgctttg
     7261 ggtatggata acccaatggc tcatgacaca gaagcagcta ttcaagttgc tgttgaagca
     7321 ctccgcacac ttattgaaaa tgataaatca caataagcga ggttttggaa gtcatcatgc
     7381 aatttattta tagtattatt ggtattttat tggtattagg aattgtgtat gcaatttctt
     7441 tcaatcgtaa gagtgtttct ctaagtttaa ttggaaaagc tcttatcgtt caattcatta
     7501 ttgcgctaat cttagtacgt atcccactag gccaacaagt tgttagtgtt gtttcaactg
     7561 gagttactaa agtaatcaac tgtggtcaag ctggtttaaa ttttgtgttt ggttcattag
     7621 cagatagtgg agcaaaaact ggttttattt tcgccattca aacgcttggt aatattgttt
     7681 tcttatctgc cctagttagt ctactttatt atgtaggaat ccttggattt gtagtaaaat
     7741 ggataggtaa gggtgttggt aaaattatga aatcctcaga ggttgagagt tttgttgccg
     7801 tagctaatat gtttcttggt caaacagaca gtccaatttt ggttagcaaa tacctaggtc
     7861 gtatgactga tagtgagata atggttgtgt tggtatcagg tatgggaagt atgtcagttt
     7921 ctattcttgg tggctacatt gcattaggca ttccaatgga atatctcttg attgcttcaa
     7981 caatggttcc tattggcagt attctcattg ctaaaatctt attgcctcaa acagaacctg
     8041 ttcaaaaaat tgatgacatt aagatggata ataaaggtaa taacgccaat gtgattgatg
     8101 caatcgctga gggtgcaagc acaggtgcac aaatggcttt ctcaattggt gctagtttga
     8161 ttgcctttgt tggtttagtt tctttgatta atatgatgtt aagtggattg ggaatccgct
     8221 tagaacaaat cttctcatat gtttttgctc catttggttt tcttatggga tttgaccaca
     8281 aaaacattct tctagaagga aaccttcttg gaagtaagtt gattttaaat gagtttgttt
     8341 cgttccaaca attgggtcac ctaatcaaat ctttagatta tcgtacagca ttggtagcaa
     8401 ctatttcact ttgtggtttt gctaatttat caagtttagg tatttgtgtt tcaggtattg
     8461 ctgttctttg tccagagaaa cgtggcaccc tagctcgact tgttttccgt gcaatgattg
     8521 gtggtattgc tgtaagtatg cttagcgcct ttatcgtcgg tattgtaact ctattctaaa
     8581 agttgacaaa agaaagaagg atagtaacgt ggaagtaaaa gatattttaa aaacggtaga
     8641 ccatactttg ctagcaacaa cagcaacatg gccagaaatc caaacaattt tagatgatgc
     8701 aatggcttat gaaacagctt cagcatgtat tccagcctct tacgtcaaaa aagcagcaga
     8761 gtacgtttca ggtaaattag ctatttgtac tgttattggg ttcccaaatg gctatagtac
     8821 aactgcagcg aaggtttttg aatgtcaaga tgctattaaa aatggtgctg atgaaattga
     8881 catggtcatt aatttgacag acgttaaaaa tggggatttt gatactgttg aagaagaaat
     8941 tcgtcaaatc aaggctgcat gtcaagacca tatcttaaaa gttatcgttg agacatgtca
     9001 attaactaaa gaagaactta tcgaactttg tggagttgtc acacgttcag gtgcagactt
     9061 tattaaaacc tctactggtt tttcgacagc aggtgctaca tttgaagatg ttgaagtgat
     9121 ggcaaaatat gtcggcgaag gtgttaaaat taaggcagca ggtggaatct catcattgga
     9181 agatgctgaa aaatttattg ctttaggagc ttcacgcttg ggtactagcc gtatcatcaa
     9241 gattgttaag aaccaaaaag ttgaagaagg cacttattaa ataatataaa ggaaatagga
     9301 aggatgctgg tctgacttcc tattttcaat tgataaagga gaagttatgt cacaatttga
     9361 tcgcattcac ttagttgtct tagattcagt gggtattgga gcggcaccag atgccaatga
     9421 ttttgtcaat gcaggagttc ctgatggtgc ttcagacacg cttggccaca tttcaaaaac
     9481 agttggactt gcggtgccaa acatggctaa aatcggactt ggtaatattc ctcgtcccca
     9541 agcgcttaag accgtccctg cagaagaaaa tccaagtggc tacgcgacga agttacaaga
     9601 agtatcactt ggtaaagata caatgacagg gcattgggag atcatgggct taaatattac
     9661 agagccattt gatactttct ggaatggttt tccagaagat atcatcacaa agattgagga
     9721 tttttcagga cgtaaagtta ttcgtgaagc gaataaacct tattcaggga cagctgttat
     9781 tgatgacttc ggaccacgcc aaatggaaac tggtgagctc atcatctata cctcagctga
     9841 tccagtccta caaattgcag ctcatgagga tatcatccct ctagaagaac tctaccgtat
     9901 ttgtgaatat gctcgtagta tcacaatgga gcgtccagct ctccttggtc gtattatcgc
     9961 tcgtccatat gtaggtgaac caggcaactt tacgcgtaca gccaatcgtc acgactatgc
    10021 agtatcacca tttgaagata ccgtattaaa taaactagat caagctggca tcgatactta
    10081 tgctgttggt aaaatcaatg atatttttaa tggttcaggc attaatcatg atatgggaca
    10141 taacaaatca aatagtcatg gtattgatac tttgataaaa acaatggggt tatcagagtt
    10201 tgaaaaaggc ttctccttta ctaatttggt tgactttgat gctctctatg gtcaccgtcg
    10261 tgatcctcat ggctatcgtg attgtcttca tgagtttgat gagcgtcttc cagagatcat
    10321 ttcagcaatg cgtgacaaag acttactttt gatcactgcc gatcatggta atgacccaac
    10381 ttatgcaggc acagaccata caagagaata cattcctctc ttagcctata gcccatcctt
    10441 cacaggaaac ggacttatcc cagtagggca tttcgcagat atttcagcaa cagttgcgga
    10501 taactttggt gttgatactg cgatgattgg agagagcttc ttgcaggact tggtataatc
    10561 atagacaaaa attttagagg ttgaaatcgg attatggttt caatcctttt ttcgttttgt
    10621 tataatagaa aaatagcggt tacaagtaca aatacagctg ttttatcagt gttgtaactt
    10681 ttctttaagc aatttacttt aatataaagt gaatgagagg ataaggtatg tttgattttt
    10741 ttaagaaaaa agttgttaaa gtgtgcttag ttatttttgg tatagtttta gtatcacttc
    10801 tgagtctagg attcttttat ttttcaaagg gacaagtact tagtcgtttt gtagcagcac
    10861 gttctcgtac gtcaggtcaa gcgtttgata atattaaaga atacatggtt tggtcagata
    10921 cgggagaatc tatcacaaat gatgaggcca attacgctaa ttttgagccc ttgtctaaaa
    10981 gtgaggcgcg taaattaggg caggaaataa aagaaggaaa caaaaatgat tcaatgtatc
    11041 taaagcgtgt aggtagtcgc ttaggtatat tccctgatta tcgtattgct aataagccta
    11101 tgtctttgac cttgaaaacc aatgttccta aattggatgt tttgttgaat caaaaaaagg
    11161 tcgcaacaag caattcagat catttttcag taacggtgga acgtttacct agaacccatt
    11221 atactgctag ccttgaaggg actagtgacg gaaaagagat taaactcaaa aaagattatg
    11281 atggtaaaaa ccaaactatt gatttatcgg tcgcttttaa atcttttaca gtaacaagta
    11341 atcttatgga tggtaatctt tattttggtg ataatcgtat tgctaaatta aaagatggta
    11401 gctattccgt agagaattat ccagtgactg atggttcaaa agcttatatc aaaaaggttt
    11461 ttaatgatgg tgagataacc tctcataaac aaaaattaat ctctatcgct gataatcaga
    11521 caatcaaatt agatgttgat ggtttattaa atgaaaaaga agctggacag aaattgataa
    11581 ctgctttcaa tcaattgata ctttatgtat caactgggca agaccctcag actttaggaa
    11641 cagtttttga aaaaggtgcc gaaaatgatt tttacaaagg attgaaagaa agtattaaag
    11701 caaaatttgt aactgataat cgtaaagcga gccatttcac tattcctaat attgtcctta
    11761 ataaaatgac acaggttggg aaagaaagtt atcaagttaa ttttgctgcc gattatgatt
    11821 ttaattatga taaatcaaca gatcctgata agaaaacata tggccacatt attcagaatt
    11881 taactggtaa ttttattatg aaaaaatcag gaaatagtta ccttatttct aatgatggta
    11941 aaaaagatat cacagtagct aaggaaacta ataaagttaa ggctgatccc gtttccattt
    12001 ttccagagaa ccttgtagga agttggaaag gtgaagttga ggacggtaca gtaacgatga
    12061 cttttgataa ggatggtaaa gttactcaga aaaaagttta caaggatagt aaatctaagg
    12121 aaagtaacca ttctgctaaa gttacgaagt tggaggataa gggaaatggg ctctaccttt
    12181 atcaatatga atcaggtact gatactacaa cttttgtaac aggaggtatc ggtggtctta
    12241 aagttaaata cgcctatgga ataaaaattg aagggaataa gatcatccca gtaatctggc
    12301 agacatctag tgatggagaa tttgattacc ataaaccact actcagtaag ccattaacta
    12361 agcaataatg attaaaagga gttggataat tattatccaa ctcctttttt ataattgttt
    12421 aaaatcttct cgaaagtaga tgaaggggcc tctacgctaa tagtttcgca agttagtgga
    12481 tgagatagag tcaatcggtg agcgtgtagc attaaccttt cattattaga agaagggttg
    12541 tagaggggat ctcctattaa aggatgtccg tgatgagata agtgaatgcg aatttgatgg
    12601 gttcttccgg tttccagtcg gcattttatg agactactat tttgggtata cttcaaaacg
    12661 tcaatgattg tcatagcatg ttgaccgttt ttagaatcaa tgattcgttt acgtctgtca
    12721 tgacggttcc gtccaatttt gtctctcaag acttgatgct taggtgaaaa ttttccttca
    12781 actaaagccc aatattcacg ccaaatttct tttcgttcta agcgttgatt gataagggga
    12841 agtataaatg gatttttagc aaataaaaca gctccactgg tctccatatc taggcgatga
    12901 acaacatagc atgtttgtcc agaataggca gatacatgat ttaacagtgc tatttcattt
    12961 ggttggttac cgtgagtttt cataccttca ggtttattaa cgataataag atgttcatcc
    13021 tcataaagac aatcaataag ctctgctctg cccagaggaa tttttttagt agggtaatcc
    13081 tcatcatcaa agattaaggt aatagtatcg ttttcttgga cgacagtttg ccaattaatg
    13141 aattcattgt ttataagtac atgtttttta acacgcaaaa aatgtctaat cttacgtgga
    13201 atcaaaaaat aatcttcaag gagttcttta acggtatgtt caccgtatgg atttctaatt
    13261 tttaatgaaa atgacatggt tttattatag cattttattg aaaattgata taataagtct
    13321 atgaaattat ttgataagtt tatagactta tttcgtgttg acgaagacaa cgacgaaatg
    13381 acaagaaaaa atgaacaaga aactagagaa gaaactagta atttggatgg cgaagaagtc
    13441 tatgatattg atgatattac ccgacctagt aaatcccagt atcagcgagg cattcgtcac
    13501 caaaagaaaa atgctaaatc tcgacctgaa tggctacaaa aagttgatcg ttacctccct
    13561 tctcctaaga atcctatccg tcgtttttgg cggcgctatc gtattgggaa gttgcttttt
    13621 atcgccttaa tggcttttat tcttatattt ggtagctatc ttttttatct ttctaaaact
    13681 gctacagtta gtgatttaca gagtgctcta aaaacaacta ccaccattta tgataagaat
    13741 aaagaatacg ctggaaaact ttcgggacaa aagggaactt atgtagagtt aaatgctatt
    13801 tctgatcatc tcaaaaatgc tgttatagca actgaagata gaacttttta tgaaaataat
    13861 ggtgtaaact ttaagcgatt ttttttggca gtagctacac ttgggaaatt tggcggaggc
    13921 tcaactatta cccagcaatt agcaaaaaat gcttacttgt cacaggacca aacgattaaa
    13981 cgcaaggcgc gtgaattctt tttagcgcta gaattgacta agaaatatag caaggctgag
    14041 attctgacca tgtatcttaa taactcttat tttggtaatg gtgtttgggg tgtggaagat
    14101 gctagtagga aatattttgg aaccagtgct gctaatttga cagtagatga ggctgctaca
    14161 cttgctggaa tgcttaaagg gccagaagtt tataatcctt attattctgt tgaaaatgca
    14221 actaaccgaa gagatactgt tttggcagcg atggttgatg caggtaaact aaccaagtct
    14281 caagctaaag aagctgcttc aattggtatg aaaaatagat tagcagatac ctatgctggt
    14341 aaaattaatg attacaggta cccatcctat tttgatgctg ttgtcaatga agccattgat
    14401 acttatggta tttcagaaaa agatattgtc aataatggct ataagattta cactgcatta
    14461 gatcaaaatt atcaatcagg aatgcaaaaa acatttgatg atactagcct cttccctgta
    14521 tctgactatg atggtcaatc tgcccaaggt gctagcgtag ctttagatcc aaaaactggt
    14581 ggtgtaagag gtttagttgg acgtgttcaa agtactaaag atgcacagtt tagaagtttt
    14641 aactacgcga ctcaatcaaa gagaagtcca gcctcaacca taaaaccttt agttgtttat
    14701 tcaccagcta ttgcttcagg atggtcaata gataaagaac ttcctaataa agttcaagat
    14761 tttcatggtt ataaaccaag taactatggt gggattgaga ctgaaagtat cccgatgtat
    14821 caagcactag ctaattctta taatattcct gctgtttata ctttagacaa actaggaatc
    14881 aataaagcct ttacttatgg caggaagttt ggtctaaata tgtcatcagc aaacaaagag
    14941 ctaggagtgg cgctaggtgg aagtataacc actaatcctt tagaaatggc acaagcttac
    15001 tcaacttttg caaatgacgg tataatgcat cgcgctcatt tgattactcg aattgagaca
    15061 gctaatggaa aattagtaaa gcaatttaca gataagccta agcgagttat tagtcgctca
    15121 gtggctagta aaatgactag tatgatgtta ggaacattct caaatggtac agctattaat
    15181 gccaatgttt atggttatac aatggcaggt aaaactggaa caaccgaaac agattttaac
    15241 cccaatttgt ctggagacca gtgggtagtc ggctatacgc ctgatgtcgt tattagtcaa
    15301 tgggttgggt ttaagaacac agataaacac cattatttga cagattctag tgcaggtaca
    15361 gcttcaaata tatttagcac tcaagcttca tatatactac cgtatactaa ggggtctagt
    15421 tttactcata ttgagaatgc ttatttccaa aacggtattg gttcagttta taatgcacaa
    15481 gatgctagta atacaacgaa ccaagaatca cgctcaatta ttaatgattt aaaagattcg
    15541 gcaagtaagg ctgcacaaga tatttctaga gcggtggaag attcaaattt ccaagagaag
    15601 gtaaaagatg cttggaatag tctaaaggac tactttagat agcttgcagg attatacaaa
    15661 tcatgttaaa atagcattta tggaggcgtt atggcacaaa agaaagcaag cctagcgtgt
    15721 acagaatgcg gaagccgaaa ttactcaatc ggagtaagta gcacccccaa gccaacacga
    15781 ctagaagtaa ataaattttg taaatattgt aagaaatata caatacacaa agaaacgcgt
    15841 tgatatagcg taacaaactg caaaaaggag aactgataat gtttgtcaaa gggatttttc
    15901 aagtattgcg tgacactact tggccaaatc gaaaacaacg ttggaaagat tttatttcca
    15961 tcttggaata cactgtattt ttcacaatcg ttatctacat ctttgacaaa ctgttagccg
    16021 caggagtcat ggacttgatt aatcgatttt aataatagat tattttagat agaaacaacc
    16081 caaaccctta gattttacta agggtttttt gtgtggaaaa taacatatat atccgactag
    16141 attataaaaa aataatcttt tgattgtttt tactagattg ttgagcaaaa aaatattaaa
    16201 aaaaaaatat ttattttaat aaaatgtaaa taaattaaag aaaagtgttg aaataaaaag
    16261 ctacaagata tataattagt taagaaactc aaattaaatt aaaataaacg tggtcctatc
    16321 ctaataaatt aggattttta actaactata taaaggagcg aatatgaata ataacgaaaa
    16381 aaaagtaaaa tactttttaa gaaaaacagc ttatggtttg gcctcaatgt cagcagcgtt
    16441 tgctgtatgt agtggtattg tacacgcgga tactagttca ggaatatcgg attcaattcc
    16501 tcataagaaa caagttaatt taggggcggt tactctgaag aatttgattt ctaaatatcg
    16561 tggtaatgac aaagctattg ctatacttct aagtagagta gatgatttta atagagcatc
    16621 acaggataca cttccacaat taattaatag tactgaagca gaaattaaca atactttacc
    16681 tcagggacga attattaaac agagtatacc agtcgtaaga ttaaaagttg agagattggg
    16741 aagtggtgca attaaggctg agtcgattaa taatattaaa gctgaatcaa ttaataaaat
    16801 tcaagctaag ccaattaaca caatcaaagc cgagtctatt aatacaatta aggctgaatc
    16861 aattcataaa attaaacctc aatcaataaa aagtactagt gctacacatg ttaaagttag
    16921 tgatcaagaa ctagctaagc agtcaagacg ttctcaagat atcattaaat cattaggttt
    16981 cctttcatca gaccaaaaag atattttagt taaatctatt agctcttcaa aagattcgca
    17041 acttattctt aaatttgtaa cacaagccac gcaactgaat aatgctgaat caacaaaagc
    17101 taggcacatg gctcaaaatg acgtggcttc aataaaaaat ataagcctcg aagtcttaga
    17161 agaatataaa gaaaaaattc aaagagctag cactaagagt caagttgatg agcttgtagc
    17221 agaagctaaa aaagttgtta attccaataa agaaacattg gtaaatcagg ccaatggtaa
    17281 aaagcaagaa attgctaagt tagaaaattt atctaacgat gaaatgttga gatataatac
    17341 tgcaattgat aatgtagtga aacagtataa tgaaggtaag ctcaatatta ctgatgcaat
    17401 gaatgcttta aatagtatta agcaagcagc acaggaagtt gcccagaaaa acttacaaaa
    17461 gcagtatgct aaaaaaattg aaagaataag tttaaaagga ttagcgttat ccaaaaaggc
    17521 taaagaaatt tatgaaaagc ataaaagtat tttgcctaca cctggatatt atgcagactc
    17581 tgtgggaact tatttgaata ggtttagaga taaacgaact ttcggaaata gaagtgtttg
    17641 gactggtcaa agtggacttg atgaagcaaa aaaaatgctt gatgaagtca aaaagctttt
    17701 aaaagaactt caagacctta ccagaggtac taaagaagat aaaaaaccag acgttaagcc
    17761 agaagccaaa ccagaggcca aaccaaatat tcaagtacct aaacaagcac ctacagaagc
    17821 tgcaaaacca gctttgtcac cagaagcctt gacaagattg actacatggt ataatcaagc
    17881 taaagatctg cttaaagatg atcaagtaaa ggacaaatat gtagatatac ttgcagttca
    17941 aaaagctgtt gaccaagctt atgatcatgt ggaagaggga aaatttatta ccactgatca
    18001 agcaaatcaa ttagctaaca agctacgtga tgctttacaa agtttagaat taaaagataa
    18061 aaaagtagcc aaaccagaag ctaagccaga agttaaacca gaagccaaac cagatgttaa
    18121 gccaggcgtt aagccagacg ttaagccaga agttaaacca gaggctaagc cagaagccaa
    18181 accagaggct aaaccagaaa ttaaaccaga cgttaagcca gaggccagac cagaggctaa
    18241 gccagaagtt aaaccagacg ttaagccaga ggccaaacca gaggctaagc cagaagttaa
    18301 accagacgtt aagccagagg ctaaaccaga agccaaacca gcaaccaaaa aatcggttaa
    18361 tactagcgga aacttggcgg ttaaaaaagc tattgaaaac aaaaagtata gtaaaaaatt
    18421 accatcaacg ggtgaagccg caagtccact cttagcaatt gtatcactaa ttgttatgtt
    18481 aagtgcaggt cttattacga tagttttaaa gcataaaaaa aattaaattt atttttaaac
    18541 ataaaccgaa aggtctatcc tttcaaaatc ttaaggcaaa cctcatcaga actttgcctt
    18601 ttttatttga attttagaat gatttagtcg gtactttaat tgacagctta ttttattttc
    18661 tttataatat atattgaaaa tagcagtcca ataagccgta aaaggctttt ttattaagga
    18721 aaggaaaact tatgttagat tcttttgata agggttggtt tgtactccaa acctactcag
    18781 gttatgaaaa taaagtaaag gaaaatctct tgcaacgtgc gcaaacttat aatatgttag
    18841 acaacatttt gcgtgtggaa atccctactc aaactgtaaa tgttgagaaa aatgggaaaa
    18901 caaaagaaat tgaagaaaat cgcttcccag gttatgtact tgtggaaatg gtaatgactg
    18961 atgaagcctg gtttgttgtt cgtaatactc caaacgttac tggatttgtt ggatcacacg
    19021 gcaatcgctc taaaccaaca ccgttgttag aagaagagat tcgttcaata ctcatctcaa
    19081 tgggacaaac cgttgatgtc tttgatacta atatcaagga aggtgatgtt gttcaaatca
    19141 ttgatggtgc tttcatcgga caagaaggac gtgtagttga gattgagaat aacaaagtta
    19201 aattaatgat caacatgttc ggctcagaaa cacaagcaga acttgagttg taccaagttg
    19261 cagaattata aaacaaaaag gctatgttag acggttgttt atcatggctt tttagttaac
    19321 atgatctaaa ctttatattc tttttcagaa aaaatagcta tattttgcta tactttaatc
    19381 aaatatcaat attagaaagt gattgcctat gttgtctgtt ggtttagttt tagagggtgg
    19441 cggaatgaga ggtctttata ctgctggagt tttagatgct tttctagatg caggaataaa
    19501 agtagatggt atcatatctg tctctgctgg tgcattgttt ggtgttaatt ttgtatctag
    19561 acaacgagag agggctttgc gatacaataa aaagtattta tcccacccta aatatatgag
    19621 tctaaggtca tggcttcgaa cagggaattt tgttaataaa gatttcacct attatgaagt
    19681 tcctatgaaa ttggatgtat ttgacgatga agcatttaaa aaatcaagta ttgattttta
    19741 cgcagttgct acagagatga catctggtaa acctgagtat tttaaaattg atagtgtttt
    19801 tgaacaaatg gaaattttac gtgctagttc agcattacca gtagtctcaa agatggttgt
    19861 ttggcagggg aaaaagtact tagatggtgg tttatctgat agtattcccg ttgattttgc
    19921 ccgtggttta ggatttgaca agttgattgt tgtgatgact aggccgctca attatcagaa
    19981 aaagccttca agtggacgat tgtataaaac tctgtatagg aaatatccta attttgtaaa
    20041 gacagcctcg aaccggtacc aacagtataa taatagcctt gaaaaggtca tgagccttga
    20101 aaaaacaggc gatctatttg caattagacc aagtaagagc ttggttattg gccgcttaga
    20161 gaagaatccg gataaacttg atagtattta tcagcttggt atgaaagatg ctaaaagtgg
    20221 gatgcctgag ctgaatagtt atctaatgaa ataaagaaaa cagtatttct caattgagga
    20281 atactgtttt gtaatatcag tcagttggtt tgacatctaa ttgtgttaaa ttagtgtgat
    20341 atttttgatg ttttaattgc ttaatgctta gcattgataa gaaactaacg atatagagac
    20401 ttgctaagaa aagcatgacc atagagacat ttgaaacgtc tagaatcatt ccgatgataa
    20461 cagacgaaaa gccacctatt gcacgaccaa catttaagat aagattgtta gctgttgaac
    20521 gaatatgatg tggatagaga cgtgtaatca tggcaccata gcctgcaaac ataccattaa
    20581 caaagaatcc gacaactgcc ccgcctataa tcatagaagg cattgttgtt gcaaattgaa
    20641 agagatagac acaaattgca gatgatagaa ggaaacaccc gtatactaaa cgcggtccaa
    20701 atttatctaa caattgacca aatactagca tgccaaggca cattccaaga atggttgcaa
    20761 tcatccataa tgatgaattc ttgacagaaa cgtttaagtt ggtttggata atagttggta
    20821 accagttcat catgccaaag aatccggcaa tctgaacagt tgtcataacc attagtgcta
    20881 ggctctgtgt ggctaataca ggagtatcaa ataaagcttt tatactgata tctagaggtt
    20941 catcatctgc gtctgtatta tattgatcta agatatcttt atcatcaata gcaaattgca
    21001 tccataggac gagaactata gggagaaggc caaagagaaa aagtccccgc caaccgagtg
    21061 ctggggctag ccatcctgct aagagtgctg agctaataga tccaacttga ccagctatac
    21121 catttagtga agaaatccgg cccattttat tagctgggac aattcctgcc ataatggcaa
    21181 tagcgatacc atattcgcca ccaacaccaa ttcctgcaat gaagcgcatg atatagagat
    21241 atgataaatt agtagtgaag aaaactaaac cagttgcgat tgaaaagatt agaatcgtcc
    21301 atttgaaaac tttaaattta ttgtagcgat cagctaataa accaaatatt agtcctccca
    21361 cgagcattcc caagttggta attgtcccaa tccaaccagc ctgtgccgca gataaatgaa
    21421 gatcagtaat gatagttgac atagaaaatg caaggaacat gatatttaaa tcatcggtcc
    21481 cggatgcgac aatggcagcg attagggcgc gccaattatt cttattgata tttaatggtg
    21541 acataagtct cctcctttaa tgatggctca cagaccaccg ttattaaata ctgtcaatag
    21601 aaaaaattct aaaacagaga tgtcatagct gcttcaactc attataatgt aaaagaaaaa
    21661 actcaagatg ctacccttga gttctataaa cgctcgcaaa aatagtagga tttctgtata
    21721 gaatcctatt ttggtcgctt atatgtctca caggacacgg ttaaagcatt atgtcatttg
    21781 gaaatagtat agagtaagtt tagtttattg tcaaggataa gtggggagag aacctgctgt
    21841 tgaggg
//
LOCUS       DATRSD010000033        21718 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRSD010000033 DATRSD010000000
VERSION     DATRSD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21718
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.033"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            167..592
                     /locus_tag="VBD85_000988"
     CDS             167..592
                     /locus_tag="VBD85_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO7133550.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNIFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKAGSEDDFFD"
     gene            610..1101
                     /locus_tag="VBD85_000989"
     CDS             610..1101
                     /locus_tag="VBD85_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133551.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVK"
     gene            1117..1926
                     /locus_tag="VBD85_000990"
     CDS             1117..1926
                     /locus_tag="VBD85_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7133552.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            1923..2750
                     /locus_tag="VBD85_000991"
     CDS             1923..2750
                     /locus_tag="VBD85_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7133553.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            2888..4537
                     /locus_tag="VBD85_000992"
     CDS             2888..4537
                     /locus_tag="VBD85_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7133554.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSSAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSDYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPKLEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLK
                     STHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            4541..5314
                     /locus_tag="VBD85_000993"
     CDS             4541..5314
                     /locus_tag="VBD85_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7133555.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            5326..6363
                     /locus_tag="VBD85_000994"
     CDS             5326..6363
                     /locus_tag="VBD85_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7133556.1"
                     /translation="MKEKQSKRLIYILLVVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNIHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKGQIL
                     KTYNRIRRNAD"
     gene            complement(6585..7082)
                     /locus_tag="VBD85_000995"
     CDS             complement(6585..7082)
                     /locus_tag="VBD85_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7133557.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     RTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(7082..8119)
                     /locus_tag="VBD85_000996"
     CDS             complement(7082..8119)
                     /locus_tag="VBD85_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO7133558.1"
                     /translation="METTLNYIKILTSTPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNDTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(8130..8585)
                     /gene="nrdI"
                     /locus_tag="VBD85_000997"
     CDS             complement(8130..8585)
                     /gene="nrdI"
                     /locus_tag="VBD85_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7133559.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNLKA"
     gene            complement(8742..11144)
                     /locus_tag="VBD85_000998"
     CDS             complement(8742..11144)
                     /locus_tag="VBD85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO7133560.1"
                     /translation="MSKHYFSKSVFALTVLTATATSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTTNNQNPQPVATEATTSDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRSVALNIGITGIVPPQILNWDKANLEG
                     KVTVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKIDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNIAGTVYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGSLLKKK
                     QI"
     gene            11404..13620
                     /locus_tag="VBD85_000999"
     CDS             11404..13620
                     /locus_tag="VBD85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO7133561.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            13630..14073
                     /locus_tag="VBD85_001000"
     CDS             13630..14073
                     /locus_tag="VBD85_001000"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO7133562.1"
                     /translation="MKIIIQRVNQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGRMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            14328..15251
                     /locus_tag="VBD85_001001"
     CDS             14328..15251
                     /locus_tag="VBD85_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO7133563.1"
                     /translation="MKRIRKSLIFVLGVVTLICLYACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQTVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            15278..17860
                     /locus_tag="VBD85_001002"
     CDS             15278..17860
                     /locus_tag="VBD85_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7133564.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIIKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRAGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIIYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     TDKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDNYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGSQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(18142..18918)
                     /locus_tag="VBD85_001003"
     CDS             complement(18142..18918)
                     /locus_tag="VBD85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7133565.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNASPTPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRIVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            19136..19609
                     /locus_tag="VBD85_001004"
     CDS             19136..19609
                     /locus_tag="VBD85_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7133566.1"
                     /translation="MTQVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYAAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            19611..19916
                     /locus_tag="VBD85_001005"
     CDS             19611..19916
                     /locus_tag="VBD85_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7133567.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            19956..21404
                     /locus_tag="VBD85_001006"
     CDS             19956..21404
                     /locus_tag="VBD85_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7133568.1"
                     /translation="MQVFLNIVNKFFDPIIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAKKNNLPTLLSVIIA
                     TVAIIFVGVLKIINSDLMKPTFDDLLGTGTTSPMTSTHMNYMMNPIIMVLDKLFDKVF
                     PGLDKYDFDAAKLNKAIGFWGSKFFIGMILGLVIGIMGNPVFSFAALGGWFSLGFTAG
                     ACLELFSLIGSWFIAAVEPLSQGITNFANGKMHGRRFNIGLDWPFIAGRAEIWACANI
                     LAPIMLVEAILLSKVGNGILPLAGIIAMGVTPALLVVTRGRLIRMITFGTLLLPLFLL
                     SGTMIAPFATELAKKVGAFPAGARAGSLITHSTLEGPMEKIFGYVIGKATTGQLSAII
                     TLIIFATAYLGLFMWYAKQMKRRNAEYAANQK"
BASE COUNT         7034 a         3512 c         4175 g         6997 t
ORIGIN      
        1 tctgttttta aatattaatt atatagaata gtccacatat agagtagaat agtccatttt
       61 taaaccgctt tcatctgtta gaatgatagt aaaggttatc ttattcaaag tattgataaa
      121 actttcagaa ctaatttagg gtaatgtaat aaataggagg agaaagatga agcggaaatt
      181 cctaatcggc agtcacggaa aacttgcgag tgggttgcag agttctattg atattctgac
      241 aggcaaggga caagagattc agacaataga tgcttacatt gatgatagtg actatactaa
      301 gtctattgta gaatttattg atgagattgc accagatgaa caggggctta tttttacaga
      361 tttgttaggt ggcagcgtta atcagaaaat ggcgactgct gtaatgaata gtgggaaaaa
      421 taatattttt ttaataacga acagcaattt agcgaccttg ttgtcattac tatttttgaa
      481 acctgaagag gaattaacta aagaagaaat tgtaaccgtt atcaatgaat ctcaagtgca
      541 gctggtagat cttagcttta aagcgggttc agaagatgac tttttcgatt aataattagg
      601 aggaaaaaaa tgattactca aattcgtgtt gacgaccgtt tgattcacgg gcaagtggca
      661 gttgtatgga ctaaagaatt aaatgcacca cttttagtgg tggctaatga tgaagctgca
      721 aaaaatgaaa tcacacagat gactcttaaa atggctgtgc ctaatggcat gaaattatta
      781 atccgttcag ttgaagaatc aatagctcta tttaaagatc cacgtgcaac tgacaagcgc
      841 atttttgtga ttgtcaattc tgttaaagat gcctgtacaa ttgctaaaaa tataaccgat
      901 ttagaagcag taaacgtcgc taatgtagga cgctttgata agtctgatcc agctacaaaa
      961 gtcaaactaa catcaagtct cttattaaat ccagaagagt tggaagccgc aaaagaatta
     1021 gccagcttac cagatttaga cgtctttaac caagtattgc cttcaaatac taaggttaac
     1081 ttgagtcagt tagtcaaata aagtgaggta tgacatatga taatgccagc gacgatggca
     1141 gctttagctg tattgatttg ttttggggga aattacttaa caggtcaaag tatgatggag
     1201 agaccacttg tagttggtct tgtaacagga atgctactag gtgatattaa ggttggtatc
     1261 ctaatgggtg cgtccctaga agcgctattt ttgggaaacg ttaatattgg gggtgttatt
     1321 gccgcagagc cagtaacagc aacagcgatg gcaacaactt ttaccattat ttcaaacatt
     1381 gatcaaaaag cagcaatgac tctagctgtt ccgattggaa tgttggcagc atttgttgtt
     1441 atgtttttga aaaacgtctt catgaatatc tttgcaccga tggtggataa agctgcagcc
     1501 gcaaaccatc aaggtaaact tgtgatgctt cactatggta catggattat ttactatctc
     1561 attatcgctt ctatttcttt cattggtatt ttagtaggta gtgggcctgt taatagtttt
     1621 gtccatcata ttcctcaaaa cctgatgaat ggtttgagtg cggcgggagg cctccttcca
     1681 gctgttggtt ttgccatgct gatgaaatta ttatggacaa ataaattagc tgttttttac
     1741 ttgcttggtt ttgttttaac agcttattta aaattaccag cggttgcagt tgctgcccta
     1801 ggagcagtta tttgcgtcat tagttcacaa cgtgatatag aattagacgc aattactaga
     1861 ggtgcgatca gtaaacagac aacctttgac tctaaagaat cagaagagga ggacttcttc
     1921 gcatgaaatc acaggataat ttgactaaag aagaccgtaa aatgcttcgc tcagtcttct
     1981 ggcgctcatg gacgatgaat gccagtcgta ctggggcaac acagtaccac gcagtagggg
     2041 ttatttatac tttattacca gtcatcaatc gtttttataa gactgataag gataaagcag
     2101 aggcactggt ccgtcataca acatggttta atgccaccat gcacattaat aattttatta
     2161 tgggtcttgt tgcatctatg gaaaagaaaa acagtgaaga tcctgatttt gatgctagtg
     2221 ctatcactgc agttaaggct tctttgatgg gacctatatc tggagttggt gattcattct
     2281 tttggggcat tttaagggtt attgcggcag gtatcggtat ctccttagca agtacaggct
     2341 cagccatggg agctgttgtc tttctactct tgtataacat tccagctttt ctgattcatt
     2401 attatagtct ctatggaggt tactctgtag gtgcagggtt tatcaaaaaa ttgtatgaat
     2461 caggtggtat taaaattgtt actaagactt caagtatgct tggtttaatg atggtaggct
     2521 caatgactgc ttcaaatgtt aaattcaaga caatcttaac ggtagcagct aaaggagcta
     2581 aggaagcagc ttccatacaa agttaccttg accaattatt tgttggcgta gtgccactgt
     2641 tggtaacgat attagccttc tggcttctac gtaaaaaagt caatatcaac tggatcatgt
     2701 ttggtattat ggtgttaggt attgtacttg gtctcttagg tatttgctaa taatctccta
     2761 gcattaaagg taagtggaac aagacgatac ttgtttagct tatctttttt tcttttgaga
     2821 gttattgtat gaaaggagtt gactatcttt attggatagc tatttgctat aataaaagga
     2881 gatggaaatg agaggatata gaatggaaga acgctttaag aaacgactac aagatgatat
     2941 ctctaaacat ttttcccgcc agtctttaat tttatccttg ttattgattg cacttttcgt
     3001 ccttttttct ttagcgcctc aacagatagg tctttataaa gatgttaatt cagtctccta
     3061 tagttataag caattgattc aaaaacatga taccttatta gatgacttag gtaaaaatag
     3121 tttgaaacca tttgtctcag gacacttagg aagtgctgat ttgagtaagc aatattatca
     3181 tttaaggaat catttacaaa gtcaaacaga acttttagta ttctcaccta atcaagaact
     3241 tttgtttgct agtaattcac atttaggtaa tttttttagt aagtctattt acattagtga
     3301 agttttagat aaggctaaaa taaatcaaag gctactaaaa attattgtgg atagtgaggg
     3361 agggcattac cttgcactca ttaagccaat tatagttaat aaaaaagttt caggttatgc
     3421 ctttttattg atgaatggga aagacttttt acttccaacg aaggctatta actcagactt
     3481 gattattgcc gatcagctaa ataatagctt tacttttact aatcgtgatt ttatctcctc
     3541 tagtttagat aaggtagata gtcaattctt aactagatat tttagttttc acgatcatcg
     3601 agcttttgtt gttaggaaag tagctctgca agataatatc ctactttaca tgtatcgtcc
     3661 cctgatacca gtaactctgg tcgtcctttt ttctctagta tcatcagtta ttatttttgt
     3721 gattttacga cgaaaatcta gagttctagc cgatcgcatc gcagtaaaaa attctagcgc
     3781 cataaatcaa atggtgctag atatggatgc catttcccgc caagaaaaat ctagtattga
     3841 gcttgatagt caagatgaat ttcaatatct atctgttcaa atcaatcaga tggtttcacg
     3901 gttaaaagat ttacatgaga aaactctcga tttggaaaca caaaagttgc tgtttgaaaa
     3961 gaggatgtta gaagctcagt ttaatcctca cttcctttat aatacccttg agacaatttt
     4021 aataaccagt gactatgatt cacaattaac agaaagaatc gttatacaac taacaaaatt
     4081 attgcgctat agccttagtg gtagcacaga agcagctgtg cttaaggatg atttagcaat
     4141 cattgaatcc taccttttga ttaatcaagt gagatttgaa gaattaactt acactatttc
     4201 agtatcccct aaacttgaac acatgcgtgt accaaaactt ttcttgttac cgctaattga
     4261 gaatgctatt aaatatggct taaaagaacg tcatgatgta gcaataaaca tagatatttg
     4321 gcaagatagt gatggaattt ggtttactgt ttcaaataat ggatcaggta ttagtttggc
     4381 taggcaaaaa gccatacgta caatgttaaa atcaactcac tcgcatcatg ggcttattaa
     4441 ttcttataga cgattacaat atcaattttc aacagtattg ttagagttta cgaagacaga
     4501 tgatgctttt cgagttagct atatagtaaa ggagtgagtt atgtatagat tacttatcgt
     4561 tgaggatgag caccttattc gtaagtggtt acgctatgct attgattatc aatcattgaa
     4621 tattttagtg gttggtgaag caaaagatgg taaagaagga gctcaattaa ttcaggaaga
     4681 gcagccagac attgttttaa gtgatattaa tatgccaatt atgacagcat tcgatatgtt
     4741 tgaggcaacc aaaggacaat cttatgccaa aattatttta tctggctatg ctgattttcc
     4801 aaatgcgcaa tcagctattc actatggcgt tcttgaattt ttaacaaaac cactagaaaa
     4861 gcaagctttg attgattgtt taaaaacaat tatggctaga attgaggaac ataaggagaa
     4921 gcacctacaa gagcatacag aattgtattt acctctacca caagctaatg accaagttcc
     4981 tgaagttatt aaagatatgc tagcatggat tcatagccat tttcatggga aaattgtgat
     5041 tagccagtta gctcacgatt taggttacag tgaaagttat ctttacacag tcactaaaaa
     5101 gcaccttcat ataactttga gtgattatat aaatcaatat cgtattaatc aagccattca
     5161 gttgatgttt agagaaccag acctaatggt ttaccaaatt gcagaagctg ttggtattta
     5221 tgattatcgt tattttgata gggtttttaa aaagtacttg ggtcagacag taaaagcgtt
     5281 taaggaagaa catattttta aacagatgga ttaggtaggt gaaagatgaa agaaaaacag
     5341 tcgaaaaggc ttatttatat actactggtt gttcccatta tttttataag tgtttttaca
     5401 tacagtatta gccagccttc taaactactt ccaccaaaag aattagttat tctaagtcca
     5461 aatagtcaag ccattttaac aggaacgatt ccagcttttg aggaaaaata cggtataaaa
     5521 gttaagctta ttcaaggtgg gacaggtcaa ctaatagata gattaagtaa ggagggtaag
     5581 cagttgaagg cggatatttt ctttggagga aattatacgc aatttgaaag tcataaggca
     5641 ttgtttgagt cttacgtatc aaagaatatt catactgtta ttccagatta tatccatccg
     5701 agtgatacgg cgacacctta tactataaat gggagtgtct tgattgtaaa taacgaatta
     5761 gctaagggac ttaccatcaa gagttatgaa gatttattac agccttcctt aaaaggtaaa
     5821 attgcctttg cagatccgaa tacttcctct agtgctttct cacaactcac taatatactc
     5881 ttggccaagg gtggttacac caatccaaaa gcgtggaact atgttaaaaa gctacaacat
     5941 aatattaatg ctatcaaatc ttctagctct tcagaagttt atcaatcagt tgcagaagga
     6001 aaaatgattg tggggttgac ttacgaagac cctagtgtca atttgcaaaa aagtggtgcc
     6061 aatgtttcta ttgtatatcc gacagaaggg acagtttttg tcccatcttc ggttgcaatt
     6121 ataaagaatg ctccttctat gaaagaagca aagttattta ttaattttat gctttcttta
     6181 gatgttcaaa atgcctttgg gcagtcaacg agtaaccgac ctattcgtaa agatgcccaa
     6241 acgagtaatg gcatgaaagc tttaaaggat attgctactc ttaaagaaga ttatcgctat
     6301 gtcactaagc ataagggcca aatccttaaa acctataatc gtattcgcag aaatgctgat
     6361 taaatgagaa tatagaaaaa ctcctcggaa tcattgattc cgaggagttt aatcatcgtc
     6421 ctcgtcgctt tcgaaatctt tcttccaata acgataaaga ctaatgcatg ccagaaatcc
     6481 ggctataatg ggaacgataa taaataagag gtcaatcact gtatccatgg gcaatatact
     6541 ccaatgataa gaagtcatta ttgtttatag tgcgtacaat aatgttaagg taaaacgtcg
     6601 gtcattataa gttttttgac atcctctaaa ctcatagatt ttgttaatat tttttcaggt
     6661 atctctttta agaagtcttt ctcaagccac catcttgcaa gagaatcttc tccaaattct
     6721 aaagatttaa gtcgagttga atgtctttta acagtttctt gaaagcttaa atcatagtaa
     6781 taagcataag tattgttggg gttatgtttt aaaatatgtt tccaaacagg cgtataccaa
     6841 tcagaacgaa gtatgccttc aaggatgatg taggaacagt tatggtaacc ataatttagt
     6901 aaattgatca aaaggggaat ggtagtagta ttttcaccat ccttagttct taacatctct
     6961 ctacgaaggt aatcctgaga taagagaagg gtatcttccc ctaattcggc ttgaagttgt
     7021 ttagctattg tagactttcc gctagctgaa tttcccctga taataatgag cgtggctacc
     7081 attagttcac catattgctt ttgagatagg catccactaa gagttcagtt gcttgaatag
     7141 aatcgatatg ggtacgttca taagaatgac tagattcaat acctgcacca agtagcgcgt
     7201 gtttaacttc cgcaccagca cgcatggcag cggaggcgtc actaccataa taaggataaa
     7261 tatcaagttt ataaggaata ttattgtttt cagctagaga aacaagatgt tgacgtaatt
     7321 cataatgata aggaccagaa gcatctttaa cacagataga gacagtatat tcgtcagttt
     7381 cttgatcgtc tcccatagct cccatatcaa ctgctagata ttctacagtt tcatttggga
     7441 tgcttgaatt tgctccatgt cctagctctt caaaggcact aaagtagaaa tgagtagtat
     7501 aaggaagttg aatgtcttct ttcttgtaaa cagaaagaag ttccattagg ataccagcgg
     7561 ataccttgtc atctaggtaa cgtgatttaa taaagccgct gcctgttatg attgtacgcg
     7621 gatcaaatga aatgaaatca ccgacctgga tgcctaaagc acgtgtttcg tcagcagttg
     7681 ttactttctc atctaaacga atttccatat ttgtttgatt acgttcagca gttccagcat
     7741 ctttgtaaac atggacacta gtttgatgaa taagagcagt tccagaaatt tctttaccat
     7801 tttttgagag gtgtatagta cagttttctc cttcaatggc attgtatgtg tatccgccaa
     7861 taaggtcaat ttttaaccga ccatctggtt tgatggccct aaccatagca ccaagtgtat
     7921 caaggtgagc agttatcatg cgatgtttag tatcattttt tccttttaga gaaaccatga
     7981 cgcctccctt gtttgtgcga attggtgagt agccaaacgc ttccaattct ttgatgatat
     8041 aggtcatgat tgtttgggta aaccctgttg gtgaaggggt tgatgtcagg attttaatgt
     8101 agtttaatgt tgtttccata ctatttcctt taggctttta agttcgataa tgtttcaacg
     8161 ataatatttg cgatacgttc aacgtcgcta gaagttcctc gcaattcaaa gtctcctaac
     8221 ataggaaatc caaaccgttc agagtattgt tttgcagtta aacagtactg attgttaaaa
     8281 tttctatttc cagagccaac aattccgata caatttttga cattattgtg tgcagctata
     8341 aaatcaccaa gtggaatggt aagaatttct tgatcgccat tgtcaatgcc atttcctccc
     8401 tcaaggtaag tgggtaatag tgctacgaaa ggttggtcaa caggaaaggt ttgatgattt
     8461 tgttccttga tattaatagt cgaaacttga cagttatgcc aagttgacag ttgttcacta
     8521 agacgtttga caaagctttg agtattgccg ctaagactga tatagactag ggttaattta
     8581 ggcatttagt actccttaaa tctaaatttc ttgttatcag tttagcaaaa tcatgtcaga
     8641 taaacaagct agagtggtat ctcaatatct gactatttag agagaaaatc tattgttgag
     8701 actaaaaact gaggccaatc aacctcagtt tttagtgatg actatatttg tttcttttta
     8761 agtaaacttc ccacgagtcc aagtattgcg acaccaaaga aagccagata agattgtttt
     8821 tcttgtccag ttttaggtaa tgttttttct ttctgttgtg tatagacagt gcctgcgata
     8881 tttggagaag cttttgcttt tgtataagca gttgcaacca tgtggtgaat tttttgtttt
     8941 tctatttggc ttaacttttc ttgatgtgat ggctcagtaa ggtctatttt ttcttgttca
     9001 gtatagacaa atcgataatc accaaatcct tcatctttgg tagaactagc gatatactga
     9061 atatccttgc tattagtaac aagattttta gctttatcgg ctgtttggaa tcgtaaatca
     9121 aggcctttaa tagactctgt gaagccccag ttattatcag ctgtaggatt gatggttttc
     9181 tctgaaataa tatagttgat aatagtttgg cgattttcta agttaagcaa acggttcatt
     9241 gtggcatttt tagccccagg gaaggtacca ctagcacggt agttattggt cacaatcata
     9301 aactcttgtt tgtctgcaac gggcttacca ttgtacatta aatcgcgtac tcgactagct
     9361 tgactattaa caacttttcc atctttattg tatttattag gttgtgtaat atcaaattta
     9421 taagtcaagc cgtcaataac gtcaaaatta taggtacgat attcggtatt gataatattt
     9481 tgtggtgctt tattgttagg gtcaatttgg ttaaattgac cagcggacat ttctaaccat
     9541 tcacgtaagt ctgctccggt cacttttaaa agtgcagtaa cattatcata aaggtaaaga
     9601 tcagctacgt ttttaattgc aacaggtcca gctggaatat cagtatatgc ggtagcatcg
     9661 ccacgtgttc cagctttaaa tggtgctgca gcagatagaa gtggtaaatt tgcctcaggt
     9721 gttcctttca attcatttgc aacgtaccaa cgttgagcgt tgttaacaat ttgaactgaa
     9781 ggatcatctt taactagtgc aaagtaactt gtaattggcg cagttgttgt acctacttgt
     9841 tggcgaacat agttgattgt gccatcgtgt gcttccttag ccaatgcaat gatacgctcg
     9901 tcagcagttg ttgagttcat atcaatttta cggattttag cactactttc ggagacttgc
     9961 cattttccat tagtataact aagtcctaaa tcaataatac caagatgatc cccgtacttg
    10021 cctgccattg taacaggtgt tccatttatt tttccattga taccatcaac tccagtgtat
    10081 ttttcataga agccagtacc gttacctgat ggaaattcag cgtgtgagtg tcccgtaaca
    10141 acggcatcca ctcccttgat gctggcaatt tgatagccaa cgttttcttc accttcttca
    10201 tatcgatcat ctccaatgcc agagtgtgat aaaacgagaa tgacatctgc tcctttagca
    10261 cgcatagtag gaacgatttc ttcgattgct ttaactgaat ctttgacagt aacctttcct
    10321 tctaggttag ccttgtccca gtttaagatt tgaggaggta caattcctgt aattccaata
    10381 tttagtgcaa cagatcttcc tgttgaatct ttgaaggttt tagtaatgat gtcataagtt
    10441 ttaaaaactg gttgatgtgt tttgaaatcc aaaacatttg cgttgaggat tggaaggttg
    10501 gctgtagcaa tgattttttt gaggtaatct aaaccgtaat taaactcatg attacctagt
    10561 gttgatgcat cataaccaag agcattcatg gcttggtaca taggatgttc ttcattttct
    10621 gcgacaggtt taacgattgc tttataagtt cctaaaggtg tcccttgaat gacatctccg
    10681 ttatcaacta agacggtatt aggattttct ttttttgctt cttcgataag gacagcagtt
    10741 tttgctaacc ctattttttg agattcttta tcttgataat aatcatagtt aactaaattg
    10801 gtgtgtaggt ctgtggttga catgatgcga aggtcaactt tttcaccttc aataggtttg
    10861 agatcactag ttgtggcttc tgtggcaaca ggctgtggat tttggttatt ggtagtggta
    10921 tttgtgcttg tggctgttga agcatcagtt attgcagttg gagtagaagt aacgtttgac
    10981 ttttcttctg caattgtgct agtgggggta gttgattcaa cctttgtagc ggttgaactt
    11041 ggtgttgtaa caatgtcttc tgcttgaaca gctaaaccac ttgttgctgt agctgttaag
    11101 acagttaaag cgaatacgct ttttgaaaaa taatgttttg acataaatct cctccattaa
    11161 aacattacat gtatattatc ctaatctaaa aactattata gcttattgaa aacgtttaat
    11221 caatctcatt tacgaattat ttttagtgat tacactaaaa acaatcgttt atttataaag
    11281 gataacttgg ttagacacat ttacttggta tttaattctt tttgaggctc taacttcttt
    11341 tatttttagg ttaaaaagtg ataaaatagg tacaatctat cactatgatt aaagaggata
    11401 actatggtca aagaaattaa tttaacagga gaagaggttg ttgcaatcac atcccagtac
    11461 atgagtgaga cggatgttgc ttttgtaaaa tttgccttaa attacgcaac cgcagctcat
    11521 tattatcaag caagaaagtc gggcgagccc tacattattc atccaatcca agtagcaggt
    11581 attcttgcag atttacacct tgatgccgtt acggtagctt gtggtttttt acatgatgtt
    11641 gtagaagata cggaaattac ccttgatgag attgaaactg attttggcaa ggatgttcgc
    11701 gatattattg acggtgtaac aaaattgggt aaagtggagt acaaatctca tgaagaacag
    11761 ttagcagaaa atcaccgtaa aatgttaatg gctatgtcta aggatatccg tgttattttg
    11821 gttaagctcg ctgatcgctt acataatatg agaaccctta aacacctcag aaaagataaa
    11881 caagagcgta tttcacgtga gactatggaa atatacgctc ctttagcgca tcgtttgggg
    11941 attagtcgta tcaagtggga attagaagat ttatcttttc gttacttgaa tgagacggaa
    12001 ttttataaga tttctcatat gatgagtgaa aaacgtcgtg aacgtgaaga gttggttgat
    12061 ataattgtcg ataaaatcag atcctatact gaggaacagg gtttatatgg tgatatttat
    12121 ggtagaccaa aacacattta ttctatttac agaaaaatgc gtgataaaaa gaaacgcttt
    12181 gatcagattt atgatttaat tgcgatacgc tgtatcatgg aaactgctag tgatgtttat
    12241 gccatggtgg ggtatatcca tgagttatgg cgtcctatgc caggaaggtt taaagattat
    12301 attgcggcac caaaggccaa tggttatcaa tctattcaca cgacggttta tggacctaaa
    12361 gggccaattg aaattcagat tcgaactaaa gaaatgcatc aagttgccga gtttggtgtt
    12421 gcagcccatt gggcctataa aaaaggaatc accagtaagg ttaatcaagc agagcaatcg
    12481 gttggtatgg gatggattca agaattagtt gagcttcaag atgaatcaaa agacgccaag
    12541 gattttgttg actctgttaa ggaagatatc tttacagaac gcatctatgt ttttacccca
    12601 aatggtgctg ttcaggaatt gccaagggaa tctggaccaa ttgattttgc ttatgctatt
    12661 cacacgcaag ttggagaaaa agctactggc gctaaagtaa atggacgtat ggttccattg
    12721 actgctaagt taaaaacagg agatgttgtt gagattatca caaaccctaa ttcatttgga
    12781 ccaagtcgtg actggattaa gattgttaag accaataaag ctcgcaataa aattcgtcag
    12841 ttcttcaaaa accaagataa agaaacttct attaataaag gtagagaatt actggttgat
    12901 tatttccaag aacaaggtta tgtgcctaac aaatatttag ataagaaaca tattgaagaa
    12961 atccttccac gtgtcagtgt taaaagtgag gaagctttgt atgcagccgt aggttttggt
    13021 gatttgagtc cgattagtat ttttaataaa ctgacggaaa aagaacgccg tgaggaggaa
    13081 cgtgctaagg ctaaagcgga agcagacgaa cttattaacg gtggtgaaat caaaaccgat
    13141 aagcgtgatg ttctcaaagt taagagtgaa aatggtgtta tcattcaagg agcttcaggt
    13201 ttattaatgc gtattgcaaa atgttgtaat cccgttcctg gagatctcat tgaaggctat
    13261 ataactaaag gtagaggcgt ggctatccat cgttcagatt gtcaaaattt aaagagtcag
    13321 gagaactacg aacaacgttt gattgatgtt gagtgggacg atgatggatc taaaaaagag
    13381 tacatggctg aaatcgatat ttatggtttg aaccgtagtg gcctccttaa tgatgttctt
    13441 caaaccttat caaatgcaac aaagctagta tcaacagtaa atgcacaacc aacaaaagat
    13501 atgaaatttg ctaatattca tgttagcttt ggaatttcaa atttagcaca attgacaact
    13561 gtggttgata aaatcaaaat tatcccagat gtttattctg ttaaacgtac gaatggttag
    13621 gagagcaaaa tgaaaatcat tattcaacgt gttaatcaag caagtgtttc tattgaagat
    13681 gatgtagtcg gatctattga gaagggttta gtacttctag ttgggattgc tccagaagat
    13741 acaacagaag atattgctta tgcagttcgt aaaattacta gcatgcgtat tttttctgat
    13801 gatgagggga gaatgaacct ttcaattcaa gacattaaag gaagcgtctt atctatctca
    13861 caatttacac tctttgcaga taccaaaaaa ggaaatcgtc cagcttttac tggggcagct
    13921 gaccctgtta aagcgaatca gttttatgat atctttaatc aagaattagc aaatcatgtt
    13981 tcggtagaaa caggtcaatt tggagctgat atgcaagtca gtctgattaa tgatggacca
    14041 gtaactatcg ttttagatac taaaaataaa taaaaaacca aagagttggg caaaatgctt
    14101 ggctcttttt attgtggttc taattatact ccttaaccca ttcatgttta tgaaaagatt
    14161 tactgtagct tggaaaggca tgaataaagg ttgttgatat gaaattaagt gcacttaaca
    14221 atattaaaaa tagaaaaata ttagatttat ggtttttcgt tgacactaaa ataagaatac
    14281 tctaaaatgt taactggtta agtatttttt attttaaagg aggtttaatg aaaaggatac
    14341 ggaaaagcct tatttttgtt ctcggagtag ttaccctaat ttgcttatat gcttgtacta
    14401 aacagagcca gcaaaaaaat ggcttgtcgg tcgtgactag cttttatcca gtatattcca
    14461 ttacaaaagc agtttctggt gatttgaatg atattaaaat gattcgatca cagtcaggca
    14521 ttcatggttt tgaaccctca tcaagtgatg ttgctgccat ttatgatgct gatctatttc
    14581 tttatcattc gcacacactc gaagcttggg cgagacgttt ggaacctagt ttgcatcact
    14641 ctaaagtatc tgtaattgaa gcttcaaaag gtatgacttt ggataaagtt catggcttag
    14701 aagatgtaga ggcagaaaaa ggagtagatg agtcaacctt gtatgaccct cacacttgga
    14761 atgaccctgt aaaagtatct gaggaagcac aactcatcgc tacacaatta gctaaaaagg
    14821 atcctaaaaa cgctaaggtt tatcaaaaaa atgctgatca atttagtgac aaggcaatgg
    14881 ctattgcaga gaagtataag ccaaaattta aagctgcaaa gtctaaatac tttgtgactt
    14941 cacatacagc attctcatac ttagctaagc gatacggatt gactcagtta ggtattgcag
    15001 gtgtctcaac cgagcaagaa cctagtgcta aaaaattagc cgaaattcag gagtttgtga
    15061 aaacatataa ggttaagact atttttgttg aagaaggagt ctcacctaaa ttagctcaaa
    15121 cagtagcttc agctactcga gttaaaattg caagtttaag tcctttagaa gcagttccca
    15181 aaaacaataa agattactta gaaaatttgg aaactaatct taaggtactt gtcaaatcgt
    15241 taaatcaata ggtagttata taaagaaagg acgcaggatg aatcgtaaaa aaacagttat
    15301 tattagtgct ttatcagtag ctctatttgg tactggtgta ggtgcttacc aattaggtag
    15361 ctataatgcc caaaaatcag acaatagtgt ttcttatgtg aaaacagata aatctgatag
    15421 caaagcacaa gcaacagctg caaataaaac gcccgaccaa atcagtaaag aagaaggcat
    15481 atctgcagaa caaatcgttg ttaaaattac tgatgatggt tatgtaactt ctcatggaga
    15541 ccattaccat tattacaatg ggaaagtgcc atatgatgcc atcattagtg aagaattgat
    15601 tattaaagac cctagctatg tctttaataa agctgatgtc attaatgaag tcaaagatgg
    15661 ttatattatc aaagttaatg gtaagtatta cctctatctt aaagaaggta gcaaacgaac
    15721 aaatgttcgt acaaaggaac aaatccaaaa gcaacgcgaa gaatggtcta agggtggttc
    15781 taagggagaa tcaggaaagc atagtagtgc aaaaactcaa gctttatcag ccagcgtacg
    15841 tgaagcgaaa gctagtggtc gctatactac agatgatggt tatgttttta gtccaacaga
    15901 tgtcattgat gacatgggag atgccttttt agtaccacat ggcgatcatt tccattatat
    15961 cccgaaagct gatttatctc catcagagtt atcagccgca caagcatatt ggaaccgcaa
    16021 gactggccga gcaggtaata gctcaaaacc atcaaattca agttcataca ttcacgcaag
    16081 tgcaccaagc ggtaatgtat caacaggaag acatgctaat gcccctattt ctattccaag
    16141 agtcactcac gcaaatcact ggtcaaaacc agctggtaat catgctacgg caccaaaaca
    16201 ccatgcacct acaaccaaac caatcaacaa agattctgct ttagacaaaa tgttgaaacg
    16261 tttatatgct caaccgttat ctgctcgtca cgtagaaagt gatggtttgg tttatgaccc
    16321 agcacaagtt aatgctttca cagcaatcgg tgtttctatt ccgcacggta atcatttcca
    16381 ctttattcac tataaggata tgtctccatt agagttagaa gcaacaagga tggtggcaga
    16441 gcatagagga catcatattg atgcattagg gaaaaaagat tctacagaga aaccaaagca
    16501 tatttctcat gaacctaata aggaacctca cacagaggaa gaacaccatg cagtaacacc
    16561 gaaagaccaa cgtaaaggca aaccaaatag ccagattatc tacagtgctc aagaaattga
    16621 agaggcaaaa aaagctggta aatacacaac atctgatggt tacatttttg atgccaaaga
    16681 tattaaaaaa gatacaggta caggttatgt cattccacat atgacacatg agcattgggt
    16741 accaaagaaa gatttatcag agtcggaatt aaaagcagct caagaatttc tttcaggaaa
    16801 atctgaagca aatcaagaca aaccaaaaac aggtaaaaca gctcaagaaa tctatgaggc
    16861 aattgaacca aaagcaattg ttaaacctga agatttatta tttggaattg cacaagcgac
    16921 agactataag aatggtacat ttgtaattcc tcataaagat cattaccatt atgtggaatt
    16981 aaaatggttt gatgaagaaa aagatctttt agctgattca gataagacat attctttaga
    17041 agactattta gctacggcta aatattacat gatgcaccca gaaaaacgtc ctaaagttga
    17101 aggatggggt aaagatgctg aaatttataa ggaaaaggac tctaataaaa cagataaacc
    17161 aagtcctgca ccaactgata ataaatcaac atcaaattct agtgataaaa acttaagtgc
    17221 tgcagaagta ttcaaacaag caaaaccaga aaaaattgta ccgcttgata aaattgctgc
    17281 tcacatggca tatgcagttg gatttgaaga tgatcaattg attgttcctc atcatgataa
    17341 ttatcataat gttcctatgg catggtttga caagggtggt ttatggaaag caccagaagg
    17401 ctatacatta caacaactct tctcaacaat taaatactac atggaacatc ctaatgaatt
    17461 accaaaagaa aaaggttggg gacacgacag tgatcataac aaaggctcaa ataaagacaa
    17521 taaagccaaa aattatgctc cagatgaaga acctgaagat tcagggaaag taactcacaa
    17581 ctatggtttt tatgatgtta ataaaggttc agacgaagaa gaaccagaaa aacaagaaga
    17641 tgaatcagag ctagatgaat atgaactagg aatggcacaa aacgctaaga aatatggtat
    17701 ggatagacaa tcttttgaaa agcaactcat ccaattatca aataaatata gtgtaagttt
    17761 tgaaagcttt aattatatta atggtagtca agttcaagtt actaaaaaag atggctctaa
    17821 agtgcttgtt gatattaaaa cactagcaga agtgaaataa taaaagagtt gagtaaactt
    17881 agctgacaac taaaaacaaa caagtcaccg tacttgtttg ttttttagtt atgatttcta
    17941 gcgtgcgaag attttaatga tatatctaat tcttcttttg aacaaccatt gttttacgct
    18001 taaaatgaga gttaatgtat ttttgtttga ggaagttttc ttgttttgac ttttataaaa
    18061 cgattaaagt tataatcact aaataaagag ccactactaa aagctgtata acaggtttct
    18121 agtagtggct cacttgtttt attacatgta attgttatta gttaaaattt tggtgaagct
    18181 actgtattgc tccttaacaa cattatcaat attatgatct gttaatacaa aatcaatatc
    18241 gtctaattga taaaaagtat aaaaatcata tttaccaaat ttttggctat caactaaaag
    18301 gaatttttca aaggaattat taagagcaat gcgctggatt tcaccttctc cctcgtcata
    18361 agtagcgata gacttttcaa aaacaccatt actactaaca aaagcttttg aaaaattaag
    18421 tgatttaata gagttaatag taacagaacc gacaaaagca ccagtaattt ctctgtattc
    18481 tcctcctaca aggataaggt caagtgttga actttgattc agaatattga aaacaggaag
    18541 gctattggtt actatcctaa ttttaaaatc aagtaattgc gtagctaatt tctctaaggt
    18601 tgttccaggc ccaataaaga ttgtttcacc atcattgatg aattgttttg caaattgggc
    18661 aatttctaat ttttcatttg tttgaatatc gtatttttca gtgtttgatt tttcataatt
    18721 ttgtggagtt ggtgatgcat ttactttttg tgcaccacca tggatgcgta ttaacagtcc
    18781 tgctttatct aattcatcca agtcacgcct aacagtcatg tctgatacat caagttcttc
    18841 catgatttca ttaacagtga caataccatt tatattgact ttttctatta ttttttgtaa
    18901 tctttctcgc tttaacataa tgacctctcc tacctcttcg ttatatatat cataccataa
    18961 atagagctca aataataata attttgaaat aataaacaat aataaacaaa aataaataaa
    19021 aaatgtcttg taatgtttta ttttgttttg tataatattg atgtagataa gttgataagg
    19081 cttgtttgcc ttacaacaaa atgtaaccgc ttaactaaaa agaaaggtgt ttgctgtgac
    19141 tcaagtgact caagatattt tatttattga tgctcattct caggaagaat tatttgactt
    19201 agtatcaaaa gcattgataa aacaacacta tgttagtcct gactaccgac aggcagtaaa
    19261 agaaagggag agagagtttc caacggggtt aaaaattgat ttaaaagatg ggactcctat
    19321 tcaatatgct gctattccac acactgaaac gcagtattgt ctagtagata gaatctttta
    19381 tgtcaaaaat agtcagccaa ttactttcaa acatatgatt aatccagaag aggagtgtag
    19441 ggttcaggat tttttcttca tcattaatag taggaatagt aaccaaagtg atattttatc
    19501 taatttgatt accttcttta ttacaaaagg aaacctagat cggctacatg agcttggcga
    19561 caataaagaa aaaattaatc attatttaat agaaaaagga gtgttttaac atgatcaaaa
    19621 tattagcagc ttgtggtgca ggcgtaaact caagtcatca aattaaagat gctatcgaga
    19681 cacaattagg agatagaggt tacaatgtac attgtgatgc tgttatggtt aaggatatca
    19741 ctgaggaaat ggtcaacaaa tatgatatct ttactcctat tgctaaaact gatttaggtt
    19801 ttaatgtacc aattccagtt gtagaagcag gacctattct ttatcgcata ccagttatga
    19861 gcgaaccggt ctttactgct ttagaacaag ttatcaaaga acacaaccta tcataacata
    19921 tttaaaattg gaattagtct taaaggagaa ggaacatgca ggtattttta aatattgtca
    19981 ataaattctt tgatccaatt atccatatgg gttcgggagt tgtgatgcta attgtcatga
    20041 caggtttagc catgatattt ggagtgaagt tttctaaagc acttgaaggt ggtattaagt
    20101 tagctattgc tcttacgggt attggtgcta ttattggtat tttaactggt gctttttccg
    20161 aatcacttca agcttttgtt aaaaatacag gaatcaatct aagcattatt gacgttggtt
    20221 gggctccatt agcaactatt acatggggat caccatatac gctttacttc ttattaatca
    20281 tgcttattgt caatattgtt atgattgtta tgaaaaaaac ggatacctta gatgttgata
    20341 tttttgatat ttggcattta tctatcacag gtcttttaat tatgtggtat gctaaaaaaa
    20401 ataatctacc aacactttta tctgtcatta ttgcaactgt agctattatc ttcgttggtg
    20461 ttctaaaaat tatcaattct gatttgatga aacctacctt tgatgattta ttggggacag
    20521 gtacaacttc tcccatgact tcaacgcata tgaattatat gatgaacccc attattatgg
    20581 ttttggataa gctttttgat aaagtttttc cagggctaga taaatatgac tttgacgcgg
    20641 ctaaattaaa taaagccatt ggattctggg gatctaagtt ctttattggt atgattttag
    20701 gacttgtcat agggattatg ggcaaccccg tgttctcatt tgcagcatta ggtggctggt
    20761 tttctctcgg ttttacagca ggtgcttgtt tagagttgtt ctctttgatt ggttcttggt
    20821 tcattgctgc agtagaacca ctttctcaag gaattactaa ttttgctaat ggtaaaatgc
    20881 atggtcgtcg tttcaatatt ggtttggact ggccatttat tgccggacgt gcagagattt
    20941 gggcatgtgc taacattctt gcaccgatta tgctagtaga agctatttta ctctctaaag
    21001 ttggtaatgg tattctaccg cttgccggta ttatcgcaat gggcgtaacc cctgctttgc
    21061 tggttgttac acgtggccgt ttaattcgta tgattacctt tggaacattg ctattaccgc
    21121 tattcctttt atcaggaaca atgattgctc catttgcaac tgaattagct aaaaaggtag
    21181 gcgcattccc agctggggcg cgagcaggtt cacttatcac tcactctaca ctagaaggcc
    21241 ctatggagaa aatctttggt tacgttattg gtaaagcgac aacaggtcaa ttatcagcga
    21301 ttataacatt aattattttt gcgacagctt atcttggttt gtttatgtgg tatgctaaac
    21361 aaatgaaacg ccgtaatgca gaatatgcag ctaatcaaaa ataaatagaa taaaaaaacc
    21421 taggagaatg ttatattttc taggtttttg atatattaaa gattttaaaa ataacatata
    21481 taattaattt tcgatcattt taataataaa aaactagtaa aattataaaa atatattgaa
    21541 aatagtgata agttggtata caatcctttt tggaaacatt actgaactcc catttttcta
    21601 gagttgatgt atgcaaccaa tattttaaga cttggagttt ctatgacatt aacctttggg
    21661 aaaccttcta taacgatacg ccgtctagga gtgataacta gtacatatat gatgctag
//
LOCUS       DATRSD010000034        21338 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRSD010000034 DATRSD010000000
VERSION     DATRSD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21338
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.034"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..796)
                     /locus_tag="VBD85_001007"
     CDS             complement(<1..796)
                     /locus_tag="VBD85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO7133569.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNL"
     gene            963..2105
                     /locus_tag="VBD85_001008"
     CDS             963..2105
                     /locus_tag="VBD85_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO7133570.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVISGADVEVHPLADG
                     GEGTVEALTLGMGGTIETIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMIKDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            2130..3386
                     /locus_tag="VBD85_001009"
     CDS             2130..3386
                     /locus_tag="VBD85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO7133571.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKDR
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            3487..4547
                     /locus_tag="VBD85_001010"
                     /pseudo
     CDS             3487..4547
                     /locus_tag="VBD85_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            complement(4602..4937)
                     /locus_tag="VBD85_001011"
     CDS             complement(4602..4937)
                     /locus_tag="VBD85_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7133572.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(5126..6154)
                     /gene="queA"
                     /locus_tag="VBD85_001012"
     CDS             complement(5126..6154)
                     /gene="queA"
                     /locus_tag="VBD85_001012"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO7133573.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKQDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGTKVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            6307..6987
                     /locus_tag="VBD85_001013"
     CDS             6307..6987
                     /locus_tag="VBD85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO7133574.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            7138..7839
                     /locus_tag="VBD85_001014"
     CDS             7138..7839
                     /locus_tag="VBD85_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO7133575.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKAGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KEEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(7911..8867)
                     /locus_tag="VBD85_001015"
     CDS             complement(7911..8867)
                     /locus_tag="VBD85_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7133576.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKFHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETVNFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            8963..9682
                     /locus_tag="VBD85_001016"
     CDS             8963..9682
                     /locus_tag="VBD85_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7133577.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLICSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            9755..10906
                     /locus_tag="VBD85_001017"
     CDS             9755..10906
                     /locus_tag="VBD85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7133578.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKVFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFMTPFLITSGLMVINVFVYLVLMSKNK"
     gene            10958..11905
                     /locus_tag="VBD85_001018"
     CDS             10958..11905
                     /locus_tag="VBD85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO7133579.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFYHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            11921..13726
                     /gene="pepF"
                     /locus_tag="VBD85_001019"
     CDS             11921..13726
                     /gene="pepF"
                     /locus_tag="VBD85_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7133580.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDNAKGFS
                     GHLLDSSQSLLEITEVELDLSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEEVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            13921..14547
                     /locus_tag="VBD85_001020"
     CDS             13921..14547
                     /locus_tag="VBD85_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO7133581.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGKLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            14621..15328
                     /locus_tag="VBD85_001021"
     CDS             14621..15328
                     /locus_tag="VBD85_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO7133582.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            15389..16318
                     /gene="prsA"
                     /locus_tag="VBD85_001022"
     CDS             15389..16318
                     /gene="prsA"
                     /locus_tag="VBD85_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO7133583.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTTLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRAFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            16560..17045
                     /locus_tag="VBD85_001023"
     CDS             16560..17045
                     /locus_tag="VBD85_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO7133584.1"
                     /translation="MATFQIKEKMFSLGGKFTITDQTGLPCYHVEGSLFPLPKTFKVF
                     DEEGHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYNETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            17061..19679
                     /gene="alaS"
                     /locus_tag="VBD85_001024"
     CDS             17061..19679
                     /gene="alaS"
                     /locus_tag="VBD85_001024"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO7133585.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLTQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKAAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFDYGADSLESNLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DHGVIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NDLTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            19761..20477
                     /locus_tag="VBD85_001025"
     CDS             19761..20477
                     /locus_tag="VBD85_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO7133586.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDKFGNYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            20565..>21338
                     /locus_tag="VBD85_001026"
     CDS             20565..>21338
                     /locus_tag="VBD85_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO7133587.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYS"
BASE COUNT         6983 a         3406 c         4154 g         6795 t
ORIGIN      
        1 cgaggttgac cttttgttga ccttttaaag ctttcagggc taagatagac gtttcatatg
       61 atctttttaa aagtgataaa gatgattttt gtccgatacc aactttatag aggccctttt
      121 cgcattcaaa ctttgatagg atatttggat aataatagtc aaacatctgg tcagaaagta
      181 aaagccagta ttgattggga taattaaagg tataaatagc agtgtcaagt ttatcaaaaa
      241 agttcaagag tgacatttca gattgactta agagtaattt ttgtttttga ctattgattt
      301 caaaagtgag tagacgatag tcagtttttt ctgataggtg gtattggttt aaaaattgat
      361 tgaggtaata gtagtctaac tctctgcctt caacaagatg atgtaagaca atagaagctt
      421 cgttttttcg cccgaaatcc agataatcta attcatgctc ttttaagatg agtcgagtca
      481 ttttttgagc aagtaaagca tattttccta cttggttggg gtttccacta atcccaatag
      541 tagctaataa ttcacaatta taatagaaag gaatattgat ccctgcttgt gtcccaaaat
      601 aagattcttg atctgttact tcaatcattt gtcctgtctg tgctactttt aaaccaattt
      661 catggaattc tccaacacgt tttggattgg tactagcgaa aatgattcct ttggtattaa
      721 taaaattaat atcttgttga caaacatctt tgatagaggt aacgatttgt tgtgcgagtt
      781 gttttcttaa tttcattcag cttacctttt gttatatata acaaatatca tatcataaaa
      841 acgctaacat ttgttctgta gtctatattt tttgttattt gattttgtta ctataattga
      901 gtgatataaa atagtgaaaa aaataacaaa gattcacaaa atattttcaa ggaggttttc
      961 aaatgaaggt agtagtagct attgattctt tgaaagggag tctttcttct ttagaagctg
     1021 gcaatgctat aaaggagtca attaatgaag taatctcagg ggcggatgtg gaagtacatc
     1081 cattagcaga cgggggagaa ggaactgttg aagctttgac gctaggtatg gggggtacga
     1141 tcgaaacaat ccctgttaag gggcctcttg gagaaaaagt acatgcgagt tatggtatta
     1201 ttccacagcg tcagttagct attattgaaa tggctgcagc tgctggtatt accctaattg
     1261 ctactgaaga gagaaatcct ctccacacaa ccacttatgg agttggagaa atgataaagg
     1321 atgctataag taaaggctgt aggcacttta ttattggtat tggaggtagt gctactaatg
     1381 atggtggggc aggtatgcta caagctttgg gatatgcatt attagataaa gataatcaag
     1441 aaatcagttt gggagcacaa ggattggcag atttaaaatc tatctctact gataaggtta
     1501 ttgaggaatt aaaagaatgt gattttaaaa ttgcatgtga tgtaacaaat cctttatgtg
     1561 gtgctcaagg ttgtagttcg atttttggac cgcaaaaagg tgctgatgaa gatatgatta
     1621 caaaaatgga tacttggtta agtaattatg ctaccttagc tacttcagtt agtgaaaaag
     1681 cagatgcaac tattgaagga acaggtgcag cgggtgggct tggatttgcc tttttagcct
     1741 ttacaaatgc taccttagaa cctggtattg atattattct atcagaaatt aatatagaaa
     1801 aagcgatttc tgaagctgat ttagtagtta ctggtgaagg tcgtttggat gggcaaacgg
     1861 tgatgggaaa agctccaatt ggcgtagcaa aattagctaa aaaatatggt aagaaagtag
     1921 tggcattttc tggatctgtc accgaagatg ctatactttg taatcaacat ggtattgatg
     1981 ctttttttcc aatcgtacga cgccttatca gcttagatga ggctatgtct aaagaggtag
     2041 cttataaaaa tatgaaagaa actgctactc aagtctttag attaatcaat ttgtataatc
     2101 attaatattg aaaggaattt tgataagtta tggaagctac atttacaaca acaggggctt
     2161 taataggatt agcgcttgct attttactga ttatcaaaaa agttcatcct gcctatagtc
     2221 tcattcttgg agcattagtt ggtggtttga ttggtggcgg tgatttagtt acaattgtca
     2281 atacaatggt attaggtgcg caagggatga tgtcatccat attacgcatt ttaacatcag
     2341 ggattttagc gggtgcttta attaaaaccg gatcagctga aaaaattgca gaaagtatca
     2401 ttaaaaaatt aggacaacag cgagccatca cagctttagc catcgcaaca atgattattt
     2461 gtgcagtagg tgtctttatt gatattgcag ttattacagt tgctccgatt gcattagcaa
     2521 taggtaaaaa agctaatctt tctaaatcta gcatactgtt agcaatgatt ggtggtggta
     2581 aggctgggaa tattatctca cccaatccta atacaattgc tgcttcagaa gcatttaagg
     2641 ttgatttaac aagcttaatg gtacaaaata ttattcctgc aattgcagct ttagtagtta
     2701 cgattatatt agcaaagatt gtttccaaga aaaataacga tatttcttat gatagtgagg
     2761 agcaagtggg ctctgatctg ccagcattct taccggctat ttctggtcca cttgtggtta
     2821 tctgcttatt agctcttcgt cctttatttg gaatcactat tgatcccttg attgcactgc
     2881 cgttgggtgg tctaattagt attttagcaa caggttacct aaaagaaacg gtaccgtttg
     2941 ttgaatacgg tttatcaaaa gttgttggcg tttcaattct tttgattgga acaggtacgc
     3001 tctcaggtat tattaaagca tctaatctac aatttgatat gattcatctc cttgaatttt
     3061 taaatatgcc gacctttatc cttgccccac tctcaggtat ttttatggga gcagcaacag
     3121 catcaacgac ttctggaaca actattgctt cacaaacgtt tgctgaaacc ttgattaaat
     3181 caggggtacc ggcggtttcg ggtgcagcta tgattcacgc aggtgcgact gttttggatt
     3241 cgctacctca tggatccttc ttccatgcga caggcggagc tgtcaatatg gctataaaag
     3301 atcgtatgaa actaattagc tatgaagctt tgattggatt aactagcaca atagttgctg
     3361 tcgtttacta ttgtttcttt gggtaaaatt tggatgaata cgtgatgttt attatcactg
     3421 tttgaataga attctcattg attttttcta attttcttac tataataaat tagtaggaga
     3481 ggtgttgtga aacaaacaaa gaaagaattt attttaggat taaccattat tattaccctt
     3541 actttattaa tcatgctgat ttttttagta atgtttaata agtccagtaa ctctaagttg
     3601 aagcagacaa aagatagaag aatatcatct acttcaacca caaggacatc cagtagctca
     3661 catgatcaaa aaattaaaag tctcttgaaa aaaagaaaat ggtcatggaa caagatatgg
     3721 aattgatgaa tgcctatagg ctctattatg attatgccca tttgtccttg aaagaggttg
     3781 ttctggccta tatgtctgaa tatggcattg aaaaaaatag tgttgctttt tcttataaaa
     3841 atttaaaaac aggacaaagg gacgctatga atgatactca ggctatgaca gcagggagca
     3901 cctataagtt acctcttaac atgttggtag tagatgcagt ggtcaagggc aagttgtcca
     3961 tggaagaacg ctttgatatt accgagactg actatgaata tattggtgaa cataataatt
     4021 atgttgctgc tttctcgggg gcaatgtcta ttgatgatat gcaaaaatac tctttagttt
     4081 attcagaaaa tacacccgcc tatgctttag ctgagcgtat tggtggcatg gatagtgctt
     4141 attcaaagtt tggtcgttat ggccaatcta aaggagacat caaaaatata caaaaaaacg
     4201 gtaataaagt gacaacagat tactatattc aagtgctaga ctatctctgg aaacatcgta
     4261 aaaagtatga tagtttaatt acttatttag aagaagcttt tccaacagat tattatcgtg
     4321 ctttgatacc atctgatgtt gttgttgctc aaaagccagg gtatgtccgt gaggcgttga
     4381 atgttggagc aattgtcaaa gaagaagtgc cttatattgt tgctatttat actgcaggtt
     4441 taggtggctc tactcaggaa gattcagaaa ttaatggagt gggactatat cagctagaac
     4501 aattatgttt tgttattaat caatggcatc gtgttaatat gaattagaaa taaatctatc
     4561 aaaataataa aaacctctta gattaactat ctaagaggtt tttataaatg tgattttata
     4621 acttggttaa gtgaagaaag ggcagaaaca gttttttgat aagattcagg actaatttct
     4681 gagaaaaaat acccgcacga ttgcaccgca ttgacaacgg catcttgtga ctcaatacct
     4741 ttgttactta gatgaataat gagttgcctt ttatcatctc ttggtttttc tcgtacaatc
     4801 cacccacgat tttccatacg ctttaaaact ggagttaggg tattcgaatt aagagatagt
     4861 gcataactaa gttccttcat atgccgatga tcctcttccc aaaggactaa caagataacg
     4921 tattggagat aagtcaattg aaattcatcc aaagcttttt ggtaaaattt attgaaaagt
     4981 cgatgtgttt cactaaccgt aaaacataat cgctcgtcta aaatacttcg attactcatt
     5041 atcatattgt agcattttgg aggcgttata gtatgtcaat cgtgttaaaa aatgataaaa
     5101 ctttttccta attgaaagcc tattcttact taacaaacat agcatcacca aagctgaaga
     5161 aacgatatcg ttcttcaact gcatggttgt aagcttctag tacaaaatca cgtccagcaa
     5221 atgctgatac taacatgaca agagtagact ttggtaagtg aaagttggtt gaaaaagcgt
     5281 ctacaacctt gaactgataa ccaggcttga taaaaatatt tgtccaccct gagtcggctt
     5341 ttaattcacc attgaattta gaaccgattg tctctaatgt gcgaatagaa gttgtaccaa
     5401 cagctacaat acgtccacca gattctttaa ctgcattgag agtatcagca gcttctttag
     5461 ataattggta aaactcagaa tgcatttcgt gttcatcaag attatcaact gaaacaggac
     5521 ggaaagttcc aagtccaacg tgaagcgtca aatagacaag cttcacacct tttgtttcaa
     5581 ttttttccaa tagttctttt gtaaaatgga gaccggctgt tggtgcagca gcagaaccat
     5641 tttcttttgc atacacagtc tgatagcggt cacgatcttc tagtttttca tgaatatatg
     5701 gcggaagtgg catttcgccc aaactttcaa ggacttctag aaaaatacca tcatatgaga
     5761 attctacaat acgcccacca tgttctaatt cctttgtgac tgttgctatt aaccgaccat
     5821 caccaaatga aactttggtc cctactcgta agcgtttagc gggtttagca agcacttccc
     5881 attgatcgcc ttctgtattt ttgagaagta ataactctac atggccgtgt gtatcttgct
     5941 tttcaccata aaggcgagcg ggcagaacac gtgtgttatt cattactaaa gcatcaccag
     6001 gcttgagttc atctaagata tgatcgaaat ggctatctgt catcgtctta ttcttatgat
     6061 caatgactaa taatttagaa gcgtctcttt tttcaagtgg tgtttgggca atgagttctt
     6121 ctggtaaata aaaatcaaaa tcgtttgtat tcatgatttc tcctatctat aagtaagctt
     6181 ttctattata tcacgttcag taaaaatgac ccaactaatc tttttattta gaatgattat
     6241 aaaaaataat tgtaatcatt ctaaataagt gatataataa ttgtcagaaa gtgagaagta
     6301 gagaagatga aagaagcaaa aaatttttcc caacaattga atattttaag agctggtgtt
     6361 cttggtgcta atgatggtat catctctgta gctggtgttg ttattggtgt tgcgagtgca
     6421 actcataacc tttggattat ttttttatca gcagcttccg cgattttagc tggtgcattt
     6481 tctatggctg gcggggagta tgttagtgtt tctactcaaa aagatactga acaagcagca
     6541 gttgcgcgtg aagagaaact actagaaaat aatccagaac ttgccaaaaa atccttagtt
     6601 gatatctact tagcaaaagg agaaagtcac gaacatgctc aatggctagt tgacaaggct
     6661 ttttcaaaga atgctattga gcatttagtt gaagaaaaat atggtattga gttcggtgaa
     6721 tatacaagcc cctggcatgc tgctatatct agttttatag cttttgccat cggatcaatt
     6781 tttccaacta taactattct gttattgcca ttttcggtac gaattgtagg aacggtaata
     6841 attgttattg tttcactatt atcaactggt tatgtaagtg ctaagttagg tcaagctcct
     6901 acagtacctg ccatgcgtcg aaatgtgatg attggttgtt tgacaatgtt ggcaacctac
     6961 gtcattggac aattattttc aatataaaat attaatcgtg gagttattgt ctaaactaga
     7021 atggacagta attcttttta gttttataac gaaattaaga agtttttatt gacaaaaatt
     7081 ggtatatacc atataatata atcataaagg tctataccaa ataaaaggag agtaaacatg
     7141 agagttatta cagttaagaa cgacattgag gggggaaaaa ttgcatttac tcttttagaa
     7201 gagaaaatga aagctggtgc tcaaacttta ggattggcaa caggaagtag cccaatcacc
     7261 ttttacgaag agattgtaaa aagtaatctt gatttttcaa atatggtttc tattaacctt
     7321 gatgaatatg ttggaattgc cgcaagtaat gatcaaagtt actcttattt tatgcataaa
     7381 catctttttg atgctaaacc tttcaaggaa aataaccttc caaacggctt ggctaaagat
     7441 ttgaaagaag aaataaaacg atacgatgct gtcataaatg ctaatccaat tgattttcaa
     7501 atccttggaa ttggtcgtaa cggtcatatt ggttttaatg aaccaggtac accttttgat
     7561 attacaacac atgtggttga tttagctcca agtacaattg aagctaatag tcgtttcttt
     7621 aatagtattg atgatgtacc aaagcaagcc ttatcaatgg gtataggctc tattatgaaa
     7681 tcaaaaacaa tcgtattagt ggcgtatggt attgaaaaag cagaagcgat tgcaagtatg
     7741 attaaaggac ctatcacaga agatatgcca gcaagtatcc tccaaaaaca tgatgatgtt
     7801 gtgattattg tggatgaagc agcggcatca aagttgtaaa gtaaataata aaaaagtaag
     7861 gatgaatctc aatgagaaaa aattcattct tactttttta ttatgcttta ttaaattgtc
     7921 cagtctaagt taggtccagc tggaataata ccagtagggt taatggtttt atggctaccg
     7981 taatagtgtt ttttgatatg atcaaaatta actgtctcag caattccagg taggttgtaa
     8041 atacgttttg tataatgcca taaatgtggg taatcatgta gcgcttttaa attacattta
     8101 aaatgaccat aataaacggt gtcaaagcga actaaagttg tgaaaaggcg aatatcagct
     8161 tcggtaaatt gttctccaac aagataatgg ttatcagata agtgtttttc taactgatct
     8221 aaagctgtga ataaggtttc aacttccttt tgataaacgt tctgagaagt ggcaaaacca
     8281 gctttataga caccattatt tatttttgga taaataaaat tattcatttc gtcaatttgt
     8341 ccttgtaagg aatcgggata gtaatcgtca gtatttccag taatatgatt aaatgcagta
     8401 tttaacatac gcataatttc agatgattca ttgttaacga ttgtatggaa ttttttatcc
     8461 caaagaacag ggactgtcac gcgtcctgta taatcacttt gacttgcttg atagatttgg
     8521 taaaggtatt ggctttggtt aatcatatca ggaataacgc ctttatattc ttgaaaagtc
     8581 caaccattct ccaacattaa agggttaaca atggagatag agatatgaga ttctagattt
     8641 ttaagctttc tcatgattaa tacacgtgat gcccaagggc aagctagtga aacgtagaga
     8701 tgatatcgtc cagattctgc tttaaaacct gcatccccac taggccctgc tgaaccatct
     8761 ttagtaaccc aatgacggaa ttgtgtaacg gttcggacga atttaccacc agttgatgca
     8821 gtatcatacc attgatcaac ccatttgcca tctactaaaa gtcccatgat aatctccttt
     8881 gtttttctat tactaattag tagtataaag attttaaaaa gaattagcaa atgtttaact
     8941 tacagtatgg taaactaagg gtatgcgttt agataaatta ttaggtcagg caggatttgg
     9001 gtctagaaat caggttaaga aattaatttg ttctaggcaa gtttctgtag atggtcaaat
     9061 tgttacaaag gataatgtta ttgtagatag tggtctacaa tcgatttttg taggaaaaga
     9121 gagggtctgc ctaaaagaaa gttcttatta ccttctttat aagccatcag gagttgtttc
     9181 agcagttaga gattcagaac ataaaacagt catagactta attagcgaaa aagataaggt
     9241 ggagggattg tatccgattg gtcgtctaga tcgtgatacg gaagggcttt taataatcac
     9301 gaataatggt ccactgggtt accgaatgtt acatccaaaa caccacgttg caaaaacata
     9361 ttatgttgaa gtaaatggtt tcttggaaag agatgctatt accttttttg aagaaggagt
     9421 tgtatttgac gatggaacaa agtgtaaacc agctgaatta acgattgata cggctaataa
     9481 tgacaaaagt accgcaagaa taactatcac agaagggaaa tttcaccaag taaaaaagat
     9541 gtttcttgca tatggcgtta aagttatata cttgaggcgt atttcttttg gtgacttgag
     9601 gcttgatatg aatttgaaac caggtcagta tcgtagattg agagattcag aggcagctat
     9661 cttaaaaagg tatttagatt agtgaaacga ctcacttgac cttgcttagt attcttcata
     9721 tacttataag tgattatttt aaaggagaaa accattgaat tcaaaacttt cattgaaatt
     9781 gactatttta tcgatttcta tctttttgat gtctcattta gcaattgctc ctgcaattcc
     9841 taaattgtat gccttttacc atactcaaaa ccctcatatt ggactagctt cagtagagag
     9901 tttagttact attccagcta tgatgattac tatttttgtt attttgagta attttgtagt
     9961 cacaaaactt ggcaaaaaaa acacggtctt acttggttta tgtttaatct taatgtctgg
    10021 gtttatctca ttttttacaa gtaatttttc acttgcgatg gctagtcgac tacttttggg
    10081 tattggtatt ggtctctata attcgctatc aattagtatc ataacggatt tatatgaggc
    10141 tgatgagaga gccagtatga ttggtcttag aacagcatca ctcaatatcg gtaaagcctt
    10201 aactacattt attgttggat tggttttagc tattggtgtg aattatattt atttggttta
    10261 ccttctggtt attcctgttt tctttttctt ttggaaaaat gttccagagg ttgaaaatca
    10321 aacacatacc cttaaagcta gtacaacatt tgatactaaa gcggctttgt tgatgctcat
    10381 cacgttctta gtaggtattg cttatatagg agcaactgtc aaaattccaa ccttactagt
    10441 gacaaaatat cactacgcga cgagcttctc tagtaatatg ttaactctcc ttgcttttag
    10501 tggtatttta gttggatctg tatttggaaa attagttaaa gtattccaag agaagacgct
    10561 attaatcatg attctagcta tgggaatagg taatgtgcta tttgctttag ctaataatca
    10621 gatcattttt atcgtagcta gtattttaat aggagctagt tttgttggca ccatgtcttc
    10681 tgtattcttt tatatttcaa aaaactatgc aaaagaacat aataacttta tcacaagcct
    10741 tgccttaaca gctggtaata taggagttat tttgacaccg cttattttaa cgaaacttcc
    10801 aagccagcta catctggaac cttttatgac accattttta ataacaagtg ggctgatggt
    10861 gattaatgtc tttgtttacc tagttttaat gtcaaaaaat aaataaaaag ttatccgaaa
    10921 ggataacttt tttcgaatag taaaagtagg agaaaaaatg ttaattgcta aagacaaaca
    10981 aggaaattta attaatctac tcgagtctca tccgggaaag ggacagtatt tttgtccaac
    11041 ttgttgttca gctgtaaggt taaaggcagg tcgaatcatg agaagacact ttgcacatat
    11101 tagtctaaaa aattgtcagt tttatcatga aaatgagtct aatgagcact tgcaattaaa
    11161 agcaaagtta tatatgagtt taagtagaga gaatgaaact atgctcgagc atcatttgcc
    11221 agaaattaat cagattgcag atttatttgt gaatgagaca ttggctttag aagttcagtg
    11281 tagccgacta tcagagcagc gactcaggga acgaacaaaa gcatatctac aggcagactt
    11341 tcaagtgaga tggttactag gggaaaaact ctggttaaag catcgtttaa caaatctaca
    11401 taaacaattt ttacaatttt ctcaaagtat cggatttcat atttgggagt tagatttgag
    11461 gttagaagta ctgagactaa aatatttgat ttacgaagat ttacgcggac atgtctatta
    11521 cttaagtaaa acttgccctc ttagtggaga cgttctagca tttttgaagt ggccatacca
    11581 atcaaaaaat ctaaattttt ataaggttaa acaagacaga aatatcagag attatgtgcg
    11641 tcagcagtta cgctatggca atcaattttg gttacgaaaa caagaaaagg cctacctttc
    11701 tggtcagaat ctattgaccc aagagctaat gatgtttttt cctcaaatac agccaccacg
    11761 tgtagatact gatttttgtc aaataacaaa ttctctaaca tcgttttatc agaattttac
    11821 aaattattat caaaaaaata agaataactt agaccaaaca ctttatcccc ctgtatttta
    11881 tgataaaata gtcactaaga attaaactgg aggatacaaa atgtcagaca atcgctcaca
    11941 tattgaagaa aaataccaat gggatttaac tactgtcttt gcgactgatg agttatggga
    12001 aactgaagtg gtagagctta ctcaagccat cgataatgca aagggttttt caggtcatct
    12061 gttggactca agccaatctt tattggagat cacggaggtt gaattagatt tatctcgccg
    12121 tttagaaaaa gtttatgtct atgcttcaat gaaaaatgac caagacacca ctgttgcgaa
    12181 atatcaagaa tttcaagcga aagcgacagc gctctatgca aaatttagtg aaacattttc
    12241 gttttacgag ccagaactat tgcaactttc agagtctgat taccagtcat tccttttaga
    12301 aatgcctgac ttgcaaaaat atgatcattt ctttgagaaa atatttgcaa ataaacctca
    12361 tgtcttatct caaaacgaag aagaattgtt agctggtgct agtgagattt ttggagcagc
    12421 tggtgagaca tttgaaatcc ttgataatgc agatatggtc ttcccagttg taaaaaatgc
    12481 aaaaggtgag gaagttgagc ttacacatgg taatttcatt tcccttatgg aatctagcga
    12541 taggacggtt cgaaaagaag catatcaagc tatgtatagc acatatgaac aattccagca
    12601 tacttacgct aaaacattac aaacaaatgt gaaatctcaa aattttaagg ctcgtgtgca
    12661 tcattatcaa tcagcacgcc aatcagctct atctgccaat tttattccag aagaggtcta
    12721 cgaaactcta attaagacag ttaatcatca tttaccgctc ttacatcgtt atatgaagtt
    12781 gcgtcaaaaa gttcttgggc tagatgattt gaagatgtat gacgtatata cacccctttc
    12841 tcaaatggat atgagtttta cttatgatga agctctaaaa aaatcagagg aggttctagc
    12901 tatttttggc gaagcatatt ctgaacgtgt tcatcgtgct tttactgaac gttggattga
    12961 tgtccatgtt aacaaaggaa agaggtcagg cgcttattca ggtggatctt atgacactaa
    13021 tgctttcatg cttcttaact ggcaagatac tttagataat ctttatacgc tggtgcatga
    13081 aactggacat agtctacact cgacgtttac tcgtgaaaat caaccctatg tttatggtga
    13141 ttattctatt ttcctagctg aaattgcatc aacaacaaat gaaaatatat taacagaaac
    13201 ccttttgaaa gaagttaaag atgataaaaa tcgctttgca attctcaatc attatttgga
    13261 cggtttcaaa ggaacaattt ttaggcaaac acagttcgct gaattcgaac atgccattca
    13321 tgtagctgat caggaggggc aagtactaac tagcgaatac ttaaacaatc tttacgctga
    13381 gcttaatgaa aaatattatg gtttgactaa agaagataat cattttattc aatatgaatg
    13441 ggcaagaatt ccacactttt actacaatta ttatgttttc caatatgcaa ccggcttcgc
    13501 agcagcaaat tatttagcag agcgtattgt aaacggtaat ccggaggata aagaggccta
    13561 cctaaattac cttaaagctg ggaattcaga ttaccctcta aacgtcattg ctaaagctgg
    13621 tgttgatatg actagtgcag actatctgga tgcagctttt agagtatttg aagagcgttt
    13681 agtggaactg gaaaatcttg tagcaaaagg cgtccataat gattaagttg aaaatatagc
    13741 tattgaagta aaaccagtca actagactgg tttttgatag tgatcagtta ttttatagtt
    13801 tgttttgaga gtggttttgg taaagttagt tagtggaaaa ctcaataggt ggaaatacct
    13861 acccaagtta tgatttttat gagaccaaaa ctgctattgt caaaaaagaa gggaaatagg
    13921 atgaagaagc ttacttttat ttgggattta gatgggacat taatagattc gtatgtacca
    13981 attatggaag ctcttgaaga aacctatcgt cattttggtt taatatttga taaagaatta
    14041 atccatgaat atattttaca ggaatcagtg gggaaattat tggtaaacct ttcagaggaa
    14101 gagcaaatac ctcatgaaaa actgaaagca tattttacaa aagaacaaga aagtcgagat
    14161 tctaaaatac atttaatgcc atatgcaaaa gagattttag aatggaccaa agaacaagat
    14221 atccccaatt ttatgtatac acataaagga gcaagtacgc attcagtgtt ggaaaccttg
    14281 cagatctctc attattttga tgaaatttta actggtgttt cgggattcga gcgaaaacca
    14341 catccacaag ggattaatta tttagttaaa cgatattctt tagataaatc aatgacttat
    14401 tacataggag atcgtccact agatttggag gttgctcaaa atgctggtat aaaatccata
    14461 aacttaaggt tagagaattc caaagaaaac tataatattt caagtctcaa agatataata
    14521 tcacttgatt tcactcgttt ggattaaatc attctcagag ttagacattt tttgctataa
    14581 taaaaaagtt attatttaaa atagaaaaga ggagccaaaa gtggttcaat cttacagtaa
    14641 aaatgctaat cataatatgc gtcgtccagt agttaaagaa gaaattgttc aatatatgcg
    14701 tcagcaccaa aaacaaaata atggctgttt agcagagtta gaagcatttg ctaaacagga
    14761 aaatatccct attatacctc atgaaacagc gacttatttt cgttttttga tgcaaacatt
    14821 acaacctaag cacattttag aaattggaac agctattggt ttttcggccc ttttgatggc
    14881 agaaaacgct cctgaagcaa agataacaac gatcgatcgt aatgaagaga tgattgcctt
    14941 ggctaaggaa aactttgcaa aatatgataa tcacaatcag ataacccttt tggaaggcga
    15001 tgctgtggat gtgttacaaa cattggataa atcttacgat tttgtattca tggattctgc
    15061 taagtctaaa tatatcgttt ttttacctca agtattaaaa catttagatg ttggaggagt
    15121 agttgtttta gatgatattt tccaaggtgg agatattgca aaacctatcg atgaagtgag
    15181 aagaggtcag cgaactatct accgaggtct gcaacgttta tttgactcga ctttgcaaca
    15241 tcctgattta actgctactt tggtaccttt gggagatggt ttattgatga ttcgtaaaaa
    15301 tgcagatcat attgtactag aagattaagg catttttaag aaattatgat ataataatgg
    15361 agttaatgac aaaaaggagt tataaatcat gaaaacaaga tcaaaacttg cagcaggctt
    15421 tttgacactt atgtcagttg ccacattagc tgcctgctca ggtaaaacat caaatggaac
    15481 aaatgttgtt acaatgaagg gcgacactat tacagtctct gatttttatg atcaagtaaa
    15541 aacatcaaaa gctgcacaac aatcaatgct tacattgatc ctctcacgtg tttttgatac
    15601 acagtatggt gataaagttt cagataaaaa agtatcagaa gcttataata agacagctaa
    15661 aggctatggt aattcatttt caagcgcact ttcacaagca ggtttgactc cggaaggtta
    15721 caaacaacaa attcgcacaa ctatgcttgt ggaatatgct gtaaaagaag cagctaagaa
    15781 agaattaaca gaagcaaact ataaagaagc atataaaaac tatactcctg aaacttctgt
    15841 acaagtaatc aaattggatg cagaagataa agctaaatct gtccttaaag atgtaaaggc
    15901 tgatggagct gattttgcaa agattgcaaa agaaaaaaca acagctactg ataagaaagt
    15961 tgagtataaa tttgattctg cagggacaac cctccctaaa gaagttatgt cagcagcctt
    16021 taagctagat aaaaatggtg tttcagatgt ggtttcaacg gttgattcaa caacttataa
    16081 aacaagttac tacatcatta aagtaactga taagacagag aaaaaatctg attggaaatc
    16141 ttacaaaaat cgcttaaaag aagttattct taaggataaa acaagcgata gagccttcca
    16201 aaataaagtg atttcaaaag ccttagaaaa agctaatgtt aagattaaag ataaagcatt
    16261 tgcgggcatc ttatcacaat atgctacaac aagtggttca tcatcactta aaaaatagtt
    16321 gaccaagtct taaaaagact gtataattaa tactattgag aatgaaattt caacgattag
    16381 atacagaaaa gtgacggttg ctgcgagtca cggataatca gaaaaagatg ctactcaatg
    16441 gggtaaaact aaaaataaat gagaatgaca agttcattga atgaaggtgg taccgcggtt
    16501 ttcgcccttc gtaatggact tgtcttttgc tatttgtcaa ctttcgagga ggaagagata
    16561 tggctacttt tcaaattaaa gaaaaaatgt tttctctagg cggtaaattt actattacag
    16621 accagactgg gcttccctgt tatcacgttg aaggaagttt gttccctcta cctaagactt
    16681 ttaaagtctt tgacgaagag gggcatctca ttagtcagat tgagaagaag gttttaagtt
    16741 ttttaccaaa gtttaatgtg acattagcca atgggaatca tttcacgatt aagaaagatt
    16801 tctcattttt gaaacctcac tacaccattg aagaccttga tatggaagtt aagggaaatt
    16861 tttgggatat ggatttccaa ctcttaaaag acaatcaagt tatagcaaac atatctcaac
    16921 agtggtttcg tatgacttca acttatcagg tagaagttta caatgaaact tacaacgatt
    16981 tgacaatttc gcttgtcata gcgatcgact atgtcaaaga gttagaaaaa aatgcatcaa
    17041 actaatttta ggagagaata atgaaagaat tatcgtctgc acaaatccgc caaatgtggt
    17101 tggatttctg gaaatccaaa ggacattccg ttgagccttc agctaacttg gttcctgtga
    17161 acgacccaac gcttctttgg atcaactcag gtgttgcaac cttgaaaaaa tattttgatg
    17221 gttcagtgat tccagaaaat ccacgtatta ccaatgcaca aaaatcaatt cgtactaatg
    17281 atattgaaaa tgttggtaaa acagcacgtc accatactat gtttgaaatg cttggtaact
    17341 tctcaattgg agactatttc cgtgatgaag ctattgagtg gggatttgaa ctcttgacaa
    17401 gtcctgaatg gtttgacttc cctaaagaca agctctacat gacttattac ccagatgaca
    17461 aggattcata taaccgttgg attgcttgtg gcgttgaacc aagtcacttg gtgccaatcg
    17521 aggataactt ctgggaaatc ggtgctggtc cttcaggtcc agatactgag attttcttcg
    17581 accgtggtga agattttgat ccagaaaata tcggacttcg ccttttggct gaagatatcg
    17641 aaaacgatcg ttacatcgaa atctggaaca tcgttctctc acaattcaat gctgatcctg
    17701 ctgtgccacg ttcagaatac aaagaattgc caaacaaaaa cattgatacg ggtgctggtc
    17761 ttgaacgtct tgcagctgtt atgcaagggg caaaaacaaa ctttgaaact gaccttttca
    17821 tgcctatcat ccgtgaagta gaaaaattgt caggtaaaac ttacgatcca gatggcgaca
    17881 acatgagttt caaggttatc gctgaccaca tccgtgcgct ttcatttgct atcggtgatg
    17941 gtgcgcttcc tggaaatgaa ggtcgtggtt acgttcttcg tcgtcttctt cgtcgtgcgg
    18001 ttatgcacgg tcgccgtctt ggcatcaacg aaactttcct ttacaaattg gttccgactg
    18061 ttggacaaat catggaaagc tactacccag aagtgcttga aaaacgtgat tttatcgaga
    18121 aaatcgttaa acgtgaggaa gaaacatttg ctcgtactat cgatgcaggt agcggtcact
    18181 tggattcatt gcttacgcag cttaaagctg aaggtaaaga tacacttgaa ggtaaagata
    18241 tcttcaaact ttatgatact tatggattcc cggttgaatt gacagaggaa ttggcagaag
    18301 atgcaggcta caagattgac cacgaaggat tcaaagcagc catgaaagaa caacaagacc
    18361 gtgcgcgtgc agctgttgtt aaggggggtt caatgggtat gcaaaatgaa accctagctg
    18421 gtattgttga agaatcacgc ttcgactacg gagctgacag ccttgaatca aatctttcag
    18481 tcatcatcgc tgacaatgaa cgtactgaag cagtttcaga aggtcaagct cttcttgtct
    18541 ttgctcaaac accattctat gctgaaatgg gtggacaggt tgctgaccac ggtgtcatca
    18601 aaaatgataa gggtgacaca gttgctgagg ttgttgatgt tcaaaaagca ccaaatggtc
    18661 aacctctaca cactgtaaac gttttggcat cactttcagt tggaacaaac tacacacttg
    18721 aaatcaacaa agagcgtcgt ttggctgttg agaaaaacca cacagcaact cacttgctcc
    18781 atgcagctct tcacaatgtt atcggtgaac acgcaactca ggctggttca ttgaacgaag
    18841 aagaattctt gcgctttgat tttactcact ttgaagcagt aagcaatgag gaacttcgtc
    18901 acattgaaca agaagttaat gagcaaattt ggaacgatct tacgatcaca acgactgaaa
    18961 ctgacgttga aaccgcaaaa gagatgggag caatggcact ttttggtgag aaatatggta
    19021 aagtggttcg tgtggttcaa attggtaatt attctgttga actttgcggt ggaactcact
    19081 taaataattc ttcagaaatc ggtctcttca agattgtcaa agaagaaggt attggttcag
    19141 gtactcgtcg tattattgca gttactggta gacaagcttt tgaagcttat cgtaaccaag
    19201 aggatgcact aaaagagatc gctgctactg taaaagctcc gcaattgaaa gatgcagcag
    19261 ctaaagtaca agctcttagc gactcacttc gtgatcttca aaaagaaaat gtagagctta
    19321 aagaaaaagc agcagctgca gcagctggtg atgtctttaa agatattcaa gaagccaagg
    19381 gcgtgcgctt cattgctagt caagttgatg ttgcagacgc aggtgcactt cgtacatttg
    19441 ctgataactg gaaacaaaaa gactactctg atgtgcttgt tctcgtagca gctattggtg
    19501 agaaggttaa tgtccttgtt gcaagcaaaa ctaaagatgt ccacgctggt aacatgatca
    19561 aaggattggc accaattgta gcaggtcgtg gtggtggtaa accagacatg gctatggcag
    19621 gtggtagcga tgcaagtaaa attgcagagt tgctagcagc agttgcagaa aatttataag
    19681 aaacacctct catttagtta gtaaataaaa cagacaaagg gcagaacatc gttctgttcc
    19741 tttgtgtata taggagaaat atggttggtt taggaacagt tatcaatgtt attcttatta
    19801 tagttggagg ctttgtaggt ttatttttga aaaatttttt gaaagaatca cttcaaaaga
    19861 gccttatgca agccatggga gttgcagttt tatttatttc gatatcaggt gttttggaga
    19921 aaatgatgtt agtcgaaaag tcacatctca tctctaatca tacaaatatg atgattatta
    19981 ctcttgctct aggtacggta cttggagagt tgctatcact agattcgtat attgataaat
    20041 ttgggaacta tcttaagcaa aaaacaggaa gtggtaacga tattaaattt gtggaggcat
    20101 tcgtcacctc aacttgtacc gtttgtattg gagcaatggc cgttgttggt agcattcaag
    20161 atggtatagc agcagatcat tcgattttat ttgctaaggg aatgttagac atgattatta
    20221 ttgcaattat gacagtgtct ttagggaaag gagctctatt ttctgcactt cctgtcgctt
    20281 tactacaagg tagccttacg attgttgctt ttttcatggg gagtctactt aatccatcat
    20341 ctttagatta ccttaactta gtaggaaata tgcttatatt ctgtgtcggt gttaatttac
    20401 tctttaacct aaatattaaa gttatcaata tgttgccagc aattatttta gctatattat
    20461 ggggaagttt tatataaaag ataaccaagt tttaactcaa acttggtttt tctttagaca
    20521 tatgttagga tgaaagaaga tatactacgg agaggatcca actcatgaat ctattatttt
    20581 ctattgatga tatgtacgta gatcatttta aagtgatgct ttattcgctt gtccgacaaa
    20641 ccaagaatcg taagttagag atatatgtct tacaaaaaac attactcaaa cgtcatacag
    20701 aacttattca atatactcaa aatttagaag ttggctatca tccaataatt gtaggtaccg
    20761 aagtatttgc tcaggcacct acaactgatc gctatccaga tacaatatat tatcgacttt
    20821 tggctcataa atttttacct gagaccttag ataggatact ttatttagat gcagatatgc
    20881 tttgcttaaa tgatttctca agtttatatg atatggagtt aggtgatcaa ctatatgcag
    20941 cggcaagtca taatactgat ggtaagtttc tagattatgt caataaatta cggttaaaaa
    21001 atgtagagct agaatcctct tatttcaata caggggtact cttgatgaac ttgcctgcta
    21061 tacgaaaagt tgttcatcaa caaactatct tagattacat aatgcaaaac cgtggtcgtc
    21121 taatcttacc agatcaggat atcttaaatg gcttatacgc taatttggtt aaaccaatac
    21181 ctgatgaaat ttataattat gatgctaggt atagtcttat ttatcaatta aaaagtcgca
    21241 atgaatggga tttagagtgg gttattaacc atactgtttt tttacatttt gcaggacgag
    21301 ataaaccatg gaaaaaagat tatagaggac gttactcg
//
LOCUS       DATRSD010000035        21332 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRSD010000035 DATRSD010000000
VERSION     DATRSD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21332
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.035"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..833
                     /locus_tag="VBD85_001027"
     CDS             <1..833
                     /locus_tag="VBD85_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7133588.1"
                     /translation="NVCGILFELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLI
                     KKLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQID
                     GVKFKQIYEDYMKLLSQGKDIAELYQKYSKEELANLGINIYQSNDIERTEERTFDEII
                     SWVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLD
                     NTSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL
                     "
     gene            1154..1603
                     /locus_tag="VBD85_001028"
     CDS             1154..1603
                     /locus_tag="VBD85_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7133589.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            1806..2126
                     /locus_tag="VBD85_001029"
     CDS             1806..2126
                     /locus_tag="VBD85_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133590.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2126..3226
                     /locus_tag="VBD85_001030"
     CDS             2126..3226
                     /locus_tag="VBD85_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7133591.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3223..3828
                     /locus_tag="VBD85_001031"
     CDS             3223..3828
                     /locus_tag="VBD85_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133592.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4034..4639
                     /locus_tag="VBD85_001032"
     CDS             4034..4639
                     /locus_tag="VBD85_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7133593.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            4632..4880
                     /locus_tag="VBD85_001033"
     CDS             4632..4880
                     /locus_tag="VBD85_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7133594.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            4970..6235
                     /locus_tag="VBD85_001034"
     CDS             4970..6235
                     /locus_tag="VBD85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7133595.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRSYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
     gene            6594..7265
                     /locus_tag="VBD85_001035"
     CDS             6594..7265
                     /locus_tag="VBD85_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7133596.1"
                     /translation="MTNYKNNLKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKHSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            7351..8022
                     /locus_tag="VBD85_001036"
     CDS             7351..8022
                     /locus_tag="VBD85_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133597.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNNNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYDGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            8006..9370
                     /locus_tag="VBD85_001037"
     CDS             8006..9370
                     /locus_tag="VBD85_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7133598.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSEKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKKDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLILIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLIEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(9478..11031)
                     /locus_tag="VBD85_001038"
     CDS             complement(9478..11031)
                     /locus_tag="VBD85_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO7133599.1"
                     /translation="MPEKMTNSENKKSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTVGCTAGAIFVVVYLHWYSKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTVRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILNQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(11125..12051)
                     /gene="arcC"
                     /locus_tag="VBD85_001039"
     CDS             complement(11125..12051)
                     /gene="arcC"
                     /locus_tag="VBD85_001039"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7133600.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGTALITSLNGLEDALDGKLGTRITK"
     gene            complement(12063..13061)
                     /gene="argF"
                     /locus_tag="VBD85_001040"
     CDS             complement(12063..13061)
                     /gene="argF"
                     /locus_tag="VBD85_001040"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7133601.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGTSIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITDDI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            13164..14459
                     /locus_tag="VBD85_001041"
     CDS             13164..14459
                     /locus_tag="VBD85_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7133602.1"
                     /translation="MLDILSEKHLERKQRIIISAIVIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            14456..15289
                     /locus_tag="VBD85_001042"
     CDS             14456..15289
                     /locus_tag="VBD85_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO7133603.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            15511..16233
                     /locus_tag="VBD85_001043"
     CDS             15511..16233
                     /locus_tag="VBD85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133604.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            16236..17750
                     /locus_tag="VBD85_001044"
     CDS             16236..17750
                     /locus_tag="VBD85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO7133605.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(17829..20369)
                     /gene="mprF"
                     /locus_tag="VBD85_001045"
     CDS             complement(17829..20369)
                     /gene="mprF"
                     /locus_tag="VBD85_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO7133606.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     NVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGVVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWIILTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGV
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(20353..21096)
                     /locus_tag="VBD85_001046"
     CDS             complement(20353..21096)
                     /locus_tag="VBD85_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133607.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            complement(21230..>21332)
                     /locus_tag="VBD85_001047"
     CDS             complement(21230..>21332)
                     /locus_tag="VBD85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEO7133608.1"
                     /translation="VLLVAVYGALTVDKKILLKQGGLPILALLTFLF"
BASE COUNT         7528 a         3979 c         3368 g         6457 t
ORIGIN      
        1 ttaatgtctg tggtatccta tttgaattag gcggaaatga cataccctca acatttaggc
       61 aagaaaacat tgatcctctc atcaataaat ggtggtatga taatgtttcg acagaaatta
      121 tccctaatct aataaaaaaa ctcaaagaaa atcccttgtt taatatcgga tttatggtaa
      181 atgatatatt agaaagaatg tataaagaga atattccaaa atcttacctt acatccgtcc
      241 cattagttat ttctcaaaaa ggaagaacaa cctattcgtt tagtatgact ggtggtcaac
      301 aaatagatgg agtgaaattc aaacagatat atgaggacta tatgaaatta ctcagtcaag
      361 gtaaggatat cgcagagtta tatcaaaaat attctaaaga agagttggca aatctaggca
      421 ttaatattta tcaatccaat gatatagaaa ggactgagga aagaactttt gatgaaatta
      481 tcagttgggt ttccaaccct tatgcaacaa gaccaattca agaaaggcac actattcaat
      541 tagagccaac aagattttca ctagaggata agaaaagaat tgaagaagct gccgctcaag
      601 gactaagcga aatcgacctt attgatttag ttgacctata tgatattaat ttagacaata
      661 caagcgtcaa tcgccatatt gtggggttat tgactaataa cacccaagta acatactatt
      721 tccaagaaca attaaataag gagttgctgt caatggctca cgctttagat aacgtacaac
      781 aggcctttat taaattatta agtgaagagg agatacgaaa atttgctctt taaagactag
      841 gtgaatcgcc tagtcttttt ttgcgtctcg ttttacaaat tagtctattg aagtaaaaaa
      901 agaagtaaaa aataaaagaa cattactcct tatactgaat atatcaagaa aaactcttga
      961 tataaatttt aatagtccaa attgatggcc atcaaaggac agattgaaac aaatatgatg
     1021 acatgacgat aaaggtcatg tcttgcatag aattgtttcg ctgtttcttt gttgtgcctt
     1081 ttggtatact cagcgttcaa tatttgctca ttttgtttcc ttctgtccaa aggacagaaa
     1141 ggagctcatc attatgagta ataaagtaca agaacgtcgt gagcgtaaaa tcaaagaagc
     1201 tattaaagct aaaaattggg atgaagtaac tcgtttgctt caacaagaac aaagtaacgc
     1261 agaacgtcgt gaccgatacc acaatagacg aatcaaagac gaaacaatag ctagcaaaaa
     1321 tgccaaaaag tctgtacgtt atgatgtcat tgcaagttct gatttgaatc ccgaagaagc
     1381 tttaatccta gaggagttaa gacaagctat tcgtgaagct aaagcatccc tatcagaaat
     1441 tgacagtaag attgttgaaa tgatagcgga acaaggttca agctataaag aaacggctcg
     1501 ctatatcact gaacattata agaaaatgag tgatgtaact gttaaatccc attattgtaa
     1561 agcgctaaaa aaattagctc ctttattaaa agcttaccgc taattcctca aatccagtgt
     1621 ctctgtcact ggattttttg taaaaaaaaa attaaaaatt caacaaataa atttgcgttt
     1681 agctgtctca ttctccttag taatgaggag gtggtcagat aacctaaaaa acaaagtcca
     1741 gctcatgacc caaaccgagc ctgtcgcatt cacactatct cttattcata aggaggattt
     1801 gccctatggc gtttaaaatg aaaaccacaa aaggtggtta tacaactaca ttggctgata
     1861 aaattatcag tcaaaaacaa ccgatttact cacttagtac cgaacttgaa ccacaacaac
     1921 ggtttgaaga aggaaaacca accggagaaa tcgtagctta taaagcatgg tttgtacagg
     1981 aagggcaaga ccctttccaa gtaaaatttg aagaaacgat tgagcttcca gcttttcaat
     2041 ccatgattca atttgacaca ctacaagcct gcgaagtaaa atataacatc tacttcaaag
     2101 caaatggtat caaggaggtt cggtaatgac ttcacaatct cgtgatgtgc taaaccaatc
     2161 ttacctgcaa tcttacctgc tccagagcct aaacatggct ctgggggctt taatgcaggg
     2221 agaaaccagc tacaccaatt cttttaatat tgttattcaa gcagacggct ttatctttgt
     2281 ccctcgtcta ccatgtgctt atatccttga tgatgacttg tacaagaaaa tctttctgat
     2341 tgccaatgct tcactttatc cacaatatac gcttctaaaa caaaacgcta cctattttgt
     2401 ccctctagaa acagatgact tacacactca gcgtggatta tttttccctt ggaaaaaggg
     2461 aatttcagaa cgtttagtca ttccagattt ggataagttt tcagctagct tgccacaggg
     2521 gaaaatccct atcatgaagt actttgaact caatttagat aaggttaatc actgggctat
     2581 tgctggaaat tcaggttctg ggaaatcgta cgctctcact tatttcttga gtgtactaaa
     2641 gcatatgtct gacttgatta tcattgaccc taaatttgat accccaagtc gatgggcaag
     2701 agaaaatcac gttgctgtta tacaccctgt tgaaaaccgt tcaaaatcag attttgtctc
     2761 acaggtcaat gaacaattaa gtcagtgtgc aactctcatt cagaaacgac aagctatctt
     2821 gtatgataat cccaatcatc aatttaccca tctaactatt gttattgatg aagtcctcgc
     2881 tctatcagag ggagtcaata agaatatcaa agaagccttt ttctccctac tctcacagat
     2941 ttccttatta ggacgtgcta ccaaaatcca tctgtttttg gtaagccagc gttttgacca
     3001 caatactatt ccaatttcag tgagggaaca gctaaacgta ttgttgcaaa ttggaaatat
     3061 caatcagaaa actactcaat ttttatttcc agacctagac ccagagggta ttgttattcc
     3121 aataggacat gggacaggta tcattcaagt tatcgataat gaacatccct accaagttct
     3181 gcccttactc tgtccaacct actacactaa acgaggtatt ctatgaccaa taaaacctac
     3241 tttatgagaa gttttaatct catactatat ttatttctat ttgcacctat ctgttctgca
     3301 tttggatggt tattcaatta tctttccgtt caattaacaa acatcacgtt tgtcaattta
     3361 gatacggtca aaagtattac ggatatcatt agttccgttt gtcatggatt tgcacttatt
     3421 tctcttctac ttgccttatt tttggttgga ttagaaataa tacaacggtt gaaaacagat
     3481 agtctttgga actatatcca atctgtctat cacacttttc tgttgcgaca cttcctgttt
     3541 cagcgtgaac gagtacagaa gataagcaat cttgaacatc agacggttac tactatcaac
     3601 ccaatccata acggatttaa ccgagctgtt cgtaagtgta tcgtagatat acgaaaagat
     3661 accatcacta tttttctaaa agtccctaga gaccaacaag ggcaaaaaat cctaaaagag
     3721 atggaagcac aattaaaaga agaaatcgtc agccaacacg cagactatta tttctcctct
     3781 cctattcgag ttcgtaacca attgtggttc actggacaaa aacgttaatc ccttggggct
     3841 gtggcttgcg ttaagcaaaa gccagacagc ccctttttat attcttttct atcatgacct
     3901 attggggtga ctacgacccc caataggtca ataatcaata atcacaaaaa attcaatttt
     3961 ttcaaaaaaa ctttgcatac gacctgtcat ttctccttag taatagaacc caaaagaaat
     4021 gaggtaactg cctatggcaa aagaacagcg ctcaaccaaa tggacttttc tcttctacga
     4081 ggaaagcgcc ccagagaact acttgaatat attagaggaa ctccatattc cgtttattct
     4141 tagtccttgg cacgataagg acgttaatag acagacggga gagttcaaga aatcgcacaa
     4201 acacggtgct ttcttctttg attcactgaa aagctattca caagtatcaa atatcatcag
     4261 tgacaaacta aacggtccag cacatgtgga ggtagttatg tctcccactg gtttatttga
     4321 ctactttacc catgcggaaa atccagataa gaccccctac aacattgagg atattgaagt
     4381 cggttgcggc tttaatttag aaaaattctt gatggagatg aactcctcgg attttataca
     4441 tgaagtagtt gatattatcg aagagaacga ctttaccgaa tttgaagaat tggtctggta
     4501 tgcccgagca aacaatacca atttattagg gcttatcatt gaacgtacat atttcttcgc
     4561 caaataccta gattcacgaa gatacaatcc taatcgactc cataattcaa acacagagga
     4621 gaaagagaat aatgaataac gaaaaattag aacaaattga acaattattg caaacactta
     4681 tcaaactcat tcaaatcaaa gaacagaaca taccactaaa gataatacaa cagcgagaat
     4741 tgctaaaaca gctaaatatc tccccaaaca cactaaaaac ttgggaacaa aaaggattaa
     4801 aaagattaga acctcctata gagggaactc gaaccgtctt ctatctacta gatgatatta
     4861 tcaacttttt acaatcctaa ttctgaaaat ttgtgctaaa atagttttat ctaaaagaaa
     4921 gagtggtaac tattttagcc tacttcaata tccatgaaga aaggcaaata tggaaactga
     4981 aacaattatt cataatggca aaaaagtcat taaaaaaatc aaaaaagata agtccatctc
     5041 atacactttg aaggggacat ttctgggaaa agatatcaaa actggtaagc aagtcactac
     5101 taccattaca gccaagacac taaaacagtt agatagagca atcattcaag cacgactaga
     5161 atttgaaaaa aatggctcaa cacgagaaaa agttatcgtc attgatacac tggaagattt
     5221 agctgaagaa tggtttaaat catataaaac ctgggttaac tcgcataata ctctgaacag
     5281 agtaagaagt tatcttgata attatattat ccctaaattt ggagattaca aaccagataa
     5341 aattgaatct gcggatatcc agctttggtt aaacgatttg gcaaagaaat caaaagagtc
     5401 cattgaatct ggtataaagc gtgctgataa aggtagtgct aaagattttg gtgctgttat
     5461 ccataagttg aaagatattt ttgactatgg aattacaaac tatggactaa tcactaatcc
     5521 tgttagcact gttaagatac cacctaagcc aaaatcaaat aagcaacgca tcatggtatt
     5581 acatgatgat ggtcttgtaa tatggcttaa ttatcttgaa agtttagata acaatagagc
     5641 caatcgtaga tttaagttaa tttgtaatac tctattagcc tccgctttgc gaataaatga
     5701 attacttgca ttgacgattg atgacctaga ttttgaaaat tcttctatta acgtcagtaa
     5761 aacattgatg tggaaaaccg caaataaaaa agatgggaca aaaggggaag ttatctgtaa
     5821 agctacacca aaaactgatg caggaaatcg ctctgtccct gtccctcccc ccataataga
     5881 aaaactcaga gatttccacc acgaaatgaa caagtatctt gaattgcaca acagacatag
     5941 aacaaaactg attttcccta caatctatgg gaactatatg tgtgacagaa acgagagaac
     6001 aactcttaag aaaaggttag taggtcttgg attaccaaac tatggtttcc atttattccg
     6061 acatactcat gcttcaatgc tacttaatgc tggaacaaat tggaaagaac tccaagtcag
     6121 aatgggacat aaatccattt caactacaat ggatacttac gctgaattag caccacaacg
     6181 aaagttagaa gctgtgggga tttatcttga aaagattgct gagctaacgc aataactact
     6241 ctaccattca ctctaccttt tattatgtag cattctagaa acattgatgt accaacgttt
     6301 ctaacagcta aaatagaaat tatttaactc tcactgaagt taaataattg aatataaaaa
     6361 agcctatgaa atcaatattt ctgggctttt tatttttaaa aataaaatct gacaaaaaag
     6421 tataaattta cctcttacct tcaggtagtt agtcattctt ttaaaactca cttttttata
     6481 actgaaaata ttgttcctaa ttttaaaaat tcattttaga ttcattttac gactatatac
     6541 tcagaagtaa caaacctaat ccaaggtttg aaaaaagaaa gaaggaagtc agtatgacaa
     6601 actataaaaa caacttaaaa aaattcatga gcaataaatt atacgtaggg ttagcttcca
     6661 ttacgctact tggtattggg ggagctatag gagtaagcgg agctgcatta gcgcataacc
     6721 atcactcaga acacatgtct tttgaacata agcatgaagg acacgataaa catcacgatc
     6781 atagagaaaa acactcacta aaaacattaa agaatatctc gacaaaaatc gattataaga
     6841 ctgccttaga aactgccaaa aaagaagctc gctcaggctt tatttcacaa attgaattaa
     6901 ataccaaagg tgatacacct ttttatgaaa tcaaagttat tgataataaa atagctaaaa
     6961 aatataaaat tgatgctaat tctgggaaga tcctttcaac aacatcagat aaccaactca
     7021 attcaaaaga tgcccaatta aaacaaccaa gtaaagatat tcagactgca ttacaagcta
     7081 ttcaagcaaa atataaaaat gcaaaaataa aagaaatttc tcttgcgaat aacaaatcaa
     7141 ataagctagt ttataaagta aaactacttg atggaaatac tcctaagatt gcccaatttg
     7201 atagtgataa agcatcattt ataaaggcaa ataagcttaa tacaactcat aaaaatcacg
     7261 actaatacta gtgtattaaa gggaatagga cctcagtact ccattccctt ttgtgatata
     7321 atggaaaaaa agccatacgg aggccaaaaa atgaaaatcc ttgttgttga agatgagttt
     7381 gacttgaacc gtagtattgt aaaactttta aaaaagcaac actacagtgt tgacagtgct
     7441 tcaaatggtg aagaagcctt acaatttgtt tcagtggcag aatatgatgt gattatctta
     7501 gatgtaatga tgccaaaaat ggacggcttc acctttttaa aactgcttcg caacaaagga
     7561 agtcaagtat ctattcttat gttgactgct cgagatgccg ttgaagatcg tatcgcaggt
     7621 ctagattttg gtgcggatga ttaccttgta aaaccatttg aatttggaga gctcatggcc
     7681 cgaattcgag ctatgttacg acgtacaaat agacaagtat cttctgatga tattcaaatt
     7741 caagatataa caatcaattt atctacaaag caagtttgga gaaacaacaa tttgattgat
     7801 ttaacagcta aggaatacga agtccttgag tatttggcac gtcacagaga ccaagtcctc
     7861 tctcgtcatc aaattcgtga acacgtttgg gattatgatt atgatggaga gtccaatatt
     7921 attgatgtcc ttatcaaaaa ccttcgtcga aaactagata acaaccgaga cggatcacta
     7981 ataaaaacta aacgaggttt aggatatgtt attccaaagt aaaataaatt accctaaaaa
     8041 gtctagcatc atcactcaaa ttaccctctg gtacagctct tttattttta tccttgttat
     8101 cggtgtatta attggttcat tttttatttc aaaaagcatt gctgaaaaca aatctgaaaa
     8161 aaatttagaa gctaaagctg tgcaaatgag tcaagcgtta gctaaaggtc atcggtatga
     8221 ggcttttgaa gatggaatat tttattcagt ctatgaccaa aatggaaaag ttatctattc
     8281 tggttttcca aaaggattta aaaaggattt agaccatcaa cataaacata aaaagaaatt
     8341 atctctattt tccatggaga atcgtacatt tcagtatgtt gatattccaa tttctgggaa
     8401 aaatcagtgg ttacgtgcta tccgtactgt tgacagactg gataagcaat taactgaatt
     8461 gttatttagt ttgggtatcg ttcttcctct tatattgatt ataataactg ttggtggcta
     8521 tttaatttta aagcgcgctt ttagaccgat tcaagaaatt actgaaaccg ctcaatttat
     8581 tacccaaaat gaggactata ctaagcgaat tatcactaaa aataacgaaa atgaattaac
     8641 tgagctagca gctgttatca atactatgtt ggcttctatt gaatcatcat ttgttcggga
     8701 aaaacaattt aacaatgatg tttctcatga actacggact cctgtaacag ttatactttc
     8761 tgaaagtgaa tacggaaaaa actacgcaga aaatttaatt gaagcaaaag aatcctttga
     8821 ggttatacac cgtcagtcac tatcaatgaa aaaattagtt gagcaacttc tagaattgac
     8881 taaggcagaa aatccattat caattcagtt agaacctctt aatttctcaa ttatgatgaa
     8941 gcaactagta tccgattcaa gtagattatt agataatacc cctatacacc ttgactctca
     9001 aatcgaagat gacctttgga ttataggtca acaaacacta ctcaaacggc tttttgataa
     9061 tctattttca aatgctatta aatttacaaa caatcatatt tctattagtc taagacagtc
     9121 cgataatcaa attgtcttct ctattaaaga taatggtcta ggtatctcag ttgatgacca
     9181 aagtaaaatt tggaaccgtt tctatcaagt ggactctgct cgaaccaagg atagtcagtc
     9241 tggaattggt ttagggctat ctttagtaaa acaaatcgct acaatacacc gtgctaaaat
     9301 atgggttgat tctaaacctg atgatggcag tcagtttacg ctcacctttc caaaattgaa
     9361 gaaagactag agaaaggaaa taaaaaaacg atagattaca cctatcgttt ttttatttaa
     9421 cttaaactca atttattata tctcttcaga tatttttgag actaatagct gaaatcttta
     9481 aagtattaaa acttgtagaa ctaacaaacc gccaccaaat attaggagga ataaaacaac
     9541 cggccacaca aattttagcc aatgggagta cttcatatct aacatctgca aggttgccat
     9601 aactagtcca gtaggtgcta aaaacaacat tgcatactgc ccaaattgat aagccattac
     9661 tactactgaa cgaggaatcc caacagtatc tgcaagaggt gctagaattg gcattgaaag
     9721 tactgctaaa cccgacgagg atggaacaac aaatcctaac acaaagtaga taaacatcat
     9781 aacaatgata aagacagggc cactaacatg tgaagctaat tttgaagctg tgtaaagcat
     9841 cgtatctgat atataacctt ggttcaaaat aatattgata ccacgcgcta aaccaatgat
     9901 taatgaaacc ccaacaaggc ttgatgcacc attaacaaac tcatcaacaa catccctttc
     9961 tccaatacca tttgccccag ttgctgttaa aaacataata ataattgtaa tagctaaaaa
    10021 ggaagaagcc atggttggaa accaccatcc ctgtgacatt actccccaaa ccatcaaagg
    10081 aaatgaaata acaaataaac ttaaaataat tttttgtctg acagtaaaga gtgagggggt
    10141 atggttagtt gtcatgcccc atttagcatt aaattctact ctatcttcat aggaatatga
    10201 aaactctgga ttcgctttta tttttttaga ataccaatgt agatacacca caacaaaaat
    10261 agcacctgca gtacacccta ctgtacgcca tgataagcct tctgtgaaac taataccagc
    10321 tgcatttgaa gcaataacag aagaaaacgg attaattgtt gaaaatgatg tcccaacaga
    10381 acttgctaag aaaatagcac ctacacaaat gatagaatca taacccatgg ctaaaaagat
    10441 tggaactaaa ataggataaa aagcaactgc ttcttcttca ataccacata gagtaccgcc
    10501 taaaaccatc aataaggaaa ctaaaaatat caataaaaac tctctacctt tggttttctt
    10561 tgttaaagct agaagaccag actcaaaagc gccactcttt ctaactactc ctatcatgcc
    10621 tccaagtacc atgataaata ccataatatc aacagcttca atggttccat ttaccatact
    10681 agtcgtcacg gagccaatgc ctgccggatt ttgcttcaaa cgtttgtaag tatttggaac
    10741 tgaaactggc ttactaatag caccagaagt aaattcttta attttaattt tgacattcat
    10801 cttatccaac tgtgtttgcg ttgctggaac atgaactgtc ttaccatttg gatctgttac
    10861 tactagtttg gatgatgctg tatcaaattg taattttgaa taggacccag caggtatcca
    10921 ccatgtagcc aaaaccgaaa agattgttaa aatgaataat attgtaaagg cacctggcat
    10981 tcgaaaacct ttactctttt tgttttcact gtttgtcatt ttttctggca tgacacgcct
    11041 cctctataat aaaaatagat tgacttcatc agaaaaagtg caaagatgaa ggttaaataa
    11101 tcatttcttt gcacttatct gagattactt agttatacgt gttcctaatt tcccatctaa
    11161 tgcatcttct aaaccattta atgaggtgat taaggctgtt cctttcggat tgtgatctaa
    11221 gaaactcata caggctatta ctttaggtaa catgcttcct tttgcaaact caccttggtt
    11281 aacgtaatca atcatttgat tctcattaac ctctgttaaa gctttttgat tttctttacc
    11341 aaattgagta taaacataat caactgcagt gagaataatc aattgatccg cagctagttc
    11401 tcctgctaac agtgcactag atttgtcttt atcaataaca gctgcaatac cttcataacc
    11461 tcccttatta atgacaggga tgcctccacc tccacctgcg ataactaggg tatcattttc
    11521 aataagtgtt ttaatgctat taatttcgat gattttttta ggttcaggtg aagcaacaac
    11581 acgacgatac cctcgtccag cgtcttctac aaaagtatac cctttctcta tagcaatctt
    11641 ttctgatgtc tccttatcat aaaaggtacc tataggcttg gtaggtgcgc taaatgcttg
    11701 atctgattca tccacctcaa tttgggtaat aattgtagca acttccttat ttattccttc
    11761 ttgacgtaat tcatttaaaa ggctctgttg gagatgatag cctatgtatc cttggctcat
    11821 tgctccacat tctggaaaag gaaaattagt tccttgacca ctctcagccg caaaattcat
    11881 tcctaaatta atggcaccaa cttgtggtcc attaccatga cttacaacaa tttcgtgtcc
    11941 cttttttatc aaggctacca atgattttgc agtatgtttt actaatctta attgttcttc
    12001 tggagaattt cctaaggcat ttcctcctag tgcaacaaca atttttgcca tgaaaattcc
    12061 tcctaatcac ctaaagttgc taccataact gctttaatag tatgcatacg attttcagct
    12121 tcttggaaaa caactgagtg agggccttca aaaacttcat ctgatacttc catttccttc
    12181 aaaccatatt tttcataaat gtcgtatcca actttagtat ctatattatg aaatgagggc
    12241 aagcaatgct cgaaaataac gtttggattt tctgtcatat taagcatttc ttgggtaata
    12301 cgatagggtt ctaaaagagc tattctttct ttccaaactt catccggttc tcccattgaa
    12361 acccaaacat ctgtatatag gacgtctgaa tctctgacac cttctgcaat atcatcagtg
    12421 atttcaatag aagcaccagt agttttcgca atttcttgac atttagagag tagttcagga
    12481 tctggctcta attctttagg acaaaccaaa tgataagtca tgcctacaat agaagtaccg
    12541 atcatcaagg cattagcgac attgttacgt ccatcaccca catacgtaaa gcgaatatct
    12601 ttatagggct tatgtaagca ttccttagca gtcagaaaat cggctagtac ttgcgtggga
    12661 tgatcagcat cggttaaacc attccacaca ggaactcctg aaaattcggc taaagtctca
    12721 actgtttctt gagaaaatcc acgatactca ataccatcat acataccacc tagcacacgc
    12781 gcagtgtctt ttgaggtctc ttttttaccc atctgactgc ctgttggacc taaataagta
    12841 acatgagctc cttgatcgtg agcagcaact tcaaaagcgc aacgtgtccg tgtagaatct
    12901 ttttcaaaaa tcaaagcgat atttttacca acaagttttt gttgttcaat tcctgcgtat
    12961 ttagcacgtt ttaaatcttc tgaaagtttt aaaagaaatt caacttctgc tgtactaaaa
    13021 tccaataaag taagaaaact acggttacgt aaatttttca ttgcttattg cctccttttt
    13081 ttttatactc ttattgtaga tagcgctttc aaaaataatc aaaattcatc aaaaatcttc
    13141 aaaaaaactt taaggacatt tgtatgctag atatattatc agaaaaacat ctagaaagaa
    13201 agcagcgtat tattatctct gcaatagtta tcgccttagc tgctcaaata aatatctcaa
    13261 tacttgctga tgggtttatt atgaccttat ctttgttcat tttgcctgtc tttttatatt
    13321 ttaatgacga cattaatcca attcttctct gtctaggaat tacttttgct tctcctatct
    13381 tcagaggaat catattaagt atcgctggcg aggctgagat tcatcaaata attgaattcg
    13441 ttttaactga tatggcgttt tatatttgtt atggcatcac cttttatact atttattggc
    13501 atcgttccta ccgtaataaa ggtacttttt tcttttctat cattatttgt gattattttg
    13561 ccaaccttgt agaaattagt tttctcataa agtttaacaa ttatactata actatattcg
    13621 ctactttatt tgccatagca cttttgcgag cttttattag ttgcgctgtg gcttatactt
    13681 atagttatct atcacttctc ttacaaaaag acgatcacga acgtcgatac tattatttta
    13741 tgtggtcaac ttcagccgtc aaaagcgagg tttattttat gcaaaaaaat atcattgaaa
    13801 ttgaaaacat catgaaaaat gcttatttat tagataaaga gttatccaaa tatcacctac
    13861 ctaaagaata ccaacacctc tctctagata tttcgcggga cgttcacgaa gttaagaaag
    13921 attaccaaaa tattattaaa ggcttaggaa cctattttag tgtaaaaaat gaatcaacta
    13981 tggcattgaa agatatcttt caaattgttt tatcttatac taggagtatt attcaatttc
    14041 ggcatcaaga tatcattatt ctcgaaaata ataaatgtaa tttgataata tcaaactatt
    14101 actatttact aaccattatt tccaatatcg ttttaaatgc agtagaagct attgataaac
    14161 aaaaaaaggg tacaatcagt gttcacacag aagaattaga agattttatt aaaattgaga
    14221 ttagtgataa cggaccagga atacctgata aaatgaaaca catgattttc aaacctggat
    14281 tttcgactaa atttgatgct aatggtgata tctatcgagg aatcgggcta tcccacgtgc
    14341 gtattttaat ggaagagcaa tatcaaggaa cgataactgt ttgtcctaat cagccaaatg
    14401 gaacaacatt tacactgtta ttccgtaaaa atcgatttaa gttaggagat aatacgtgaa
    14461 attctatatt attgatgatg accccactat cactatgatt ttacaagata ttattgaaga
    14521 agatttcaat aatacagtag tcagagtgaa taatgtctcc agtaaggcat acaacgaatt
    14581 gctgattgca gatgttgata tcgtattgat tgatttatta atgccaatat tagacggtgt
    14641 aacattagtc caaaaaatct ataaacagcg ttccgattta aaatttatta tgatttccca
    14701 agtcaaagat aatgatttac gacaagaagc atataaagca ggaatcgaat tttttattaa
    14761 taaacccatt aatattattg aagtaaaaag cgtcgtgaaa cgtgtcactg atactatcga
    14821 aatgcaaaaa aaattaaata caatccaaaa tctcctagaa aatacgccat catatcaaaa
    14881 gcctataacg actagtaacc tcactaaaat ccgttcaata ctcagttatt taggtattac
    14941 ttcagagaca gcctacacag atattcttaa catctgtgaa cttttattaa aacaagaatt
    15001 aaattttgcc caatttgatt ttcaaaaaga actttctatc gatgaacacc aacaaaaaat
    15061 cattttacaa cgtattcgtc gtgccgttaa aaaagctatg ataaatatgg ctcatctgta
    15121 cattgatgat tttgaaaatg aattaacact gcaatatgct aatgcattgt ttggatttca
    15181 gaatatccat aatgaggcac aacttattca aggaaaaagc atgtatggtg gtaaaatttc
    15241 acttaaacgt tttttcgatg aacttatttt acagagtaaa acgttttaag acttgtttaa
    15301 gcatggcctt atatactata accatcctaa aacttttctt ttcataaatc tccaacaaac
    15361 accttgtcta ccgcaaggtg tttgtttttg cttctagttt atcgtttctg acaatttaaa
    15421 tgtcagatag ttgtttgaat gtctacttta tgctaatata accctaatat catacgacct
    15481 acactcgtta cgcactagaa agtaaattac atgatttcat ttgaaaatgt tagtaaatca
    15541 tatggtgatc ataccatcat tgataatatt agttgccata ttcaaagagg agaattcttt
    15601 gtccttgtgg gggccagtgg cagtggtaaa actaccatcc tcaagatgat taatcgtctc
    15661 atcgaaccaa gccaaggcgc tattacttta gatggggaaa acattacatc acttgatttg
    15721 cgacaattac gcttagaaac tggttatgtc ttgcaacaaa ttgcactttt ccctaattta
    15781 acagttggag aaaatataga acttatccct gaaatgaagg gctggtcaaa aggtgaccaa
    15841 aaaaaagccg ctagcgacct tcttgataag gtgggtttac ctgctaagga ctactttaat
    15901 cgctatcctc atgagctctc tggtggtgaa caacaacgca taggtatctt acgagcaatc
    15961 gttgctaaac ctaaggtact acttatggat gagccatttt ctgctttaga ccctatctca
    16021 agacgtcaat tgcaagatat caccaaacaa ttacagtctg aattgggtat tacattagtg
    16081 tttgtcaccc atgacatgaa agaagctatg cgtcttgcag atagaatttg tgttattaag
    16141 gaaggtaaga tagtgcaatt ggatagacca gaaataattc aaaataatcc ctctgaccaa
    16201 tttgttagaa ccctatttga agaggagaac tgagtatgac aacactaatc actacctttc
    16261 aagagcgttt tggagattgg actcaaagtc ttattgaaca cctccagcta tcactactaa
    16321 cacttatttt agcaacctta attgctattc cactcggtat catcattagt cattataaaa
    16381 aaatcagtca tgttgtcctt cagataactg ggatatttca aacaattcct tctttagcct
    16441 tgcttggttt atttatacct tttatgggaa ttggaactgt accagctgtc gttgcactaa
    16501 ttatttacgc tctatttcca attttacaaa atactgtaac agcactgatg caaattgatg
    16561 ctaatctcat tgaggcagca actgcttttg gaatgacacg ttgggaaagg cttaaaaagt
    16621 ttgagttagc tttaagtatg cctgttatca tctcaggcat tagaactgcc tctgttatga
    16681 ttattggtac agctacatta gctagtctta tcggagctgg tggccttgga tcatttattt
    16741 tgttagggat tgatcgcaac aatccttctc taattctcat aggggcaatc tcttctgctg
    16801 tcttagctat tatatttagt ggtttaattg gattactaga aaaggctaga ttacgaacca
    16861 tagccgtttc tggtatactt ttactagcag gcttaggact atcctatgct ccaaaatgga
    16921 tgcctggaac aaatactgca acgataactg ttgcagggaa gttaggaact gaaccagata
    16981 ttttaattaa tatgtacaaa gaacttattg aggatcagac agatatcaaa gttaagctta
    17041 aacctaattt cggaaaaaca actttcttat atcaagccct aaaatctggt gacattgatc
    17101 tttacccaga gtttacaggt actattacat caagtttact taaaaatcct cctaaagtaa
    17161 gtaataaccc taaacaggtt tacaacttag ccaaaaacgg tattttaaaa caagataagt
    17221 tgtcactttt gtcacctatg gcttatcaaa acacctacgc tgtagctgtt aaaaaagatt
    17281 acgctgaagc taatcaactc aaaaatatca gtgacctaaa aaaattagat aaactaaaag
    17341 ctggtttcac tttagaattt aaggatagag aggatgggag tattggttta cagaaacatt
    17401 atggtttaaa ccttgatatc tcaaccttag aacctgcttt acgataccaa gctatcaata
    17461 gtaaagatgt caatattatt gacgcttact ctaccgatag tgaattaatc cagtatcaac
    17521 ttcaaattct caaagacgat aagcatctat ttccgccata tcaaggagca ccgctactaa
    17581 ggcaagatac gattaaaaaa taccctcaag taaaaaaagc tctcaataaa cttgctggtc
    17641 atatcactga aaaagaaatg caagaaatga actaccaagt agctgtaaag cataaatcag
    17701 cagctacagt agctaaacaa tatttaaaag cacatcatat tataaagtag attcaagcaa
    17761 tcaatggtat taaatgattt aaaaagagca tgtcgcatga catgctcttt ttaattgcca
    17821 aataaagctt atttaacaat cttaatttta ctatcttcca ttaatagggc acaaatagca
    17881 caaattaacc aggacgaacg agaacaagaa atataacgtt ccgaccacaa tggtgtaaac
    17941 ttcttcttat acttgtgtaa accattaaat gagtagaaat gactaccgaa atgatagaca
    18001 agatacgcca ttctctcttt agcaaaactt gtttcaacgc gaccaactcc tgataaaggt
    18061 gccatcccca aatcaaaata gtggtatccc ttctcttgat aataagagaa taatgataaa
    18121 aagaggaaat ccataacgcc attcggaatt ttctgtttat cgtgacgcat taaatcaata
    18181 gagataatac tcttttcata gtttggcata atattagcaa aagcaacaac ttcgtgttca
    18241 gcatttttta ccaaagctat tggggcttgt tggaaataat ctttattaaa atatcctaat
    18301 gagaaacctt tttcaggacg tccttctaac caagtattag aaatctcttc caaactattt
    18361 agtagctctt gactatgtgg cgattgtaca acttcgaaat agaaaccatc cttttcaact
    18421 ctatttaggg catttctgaa aggtttgtac ttattccctt taagagtaaa cgtttctaaa
    18481 ttaactaaag catcctcacc aactttcata aagtcaaaac catactcatg taaaagtagt
    18541 gtcaacttct gtccaatact gtaaaaaaca aggtcatagt ctagcttatc agcatcatca
    18601 ataaacgatt caatagcttc acgaatataa gtgtcatcac cggctggttc acccataata
    18661 agacatttat tattgacaat tacaaattgg aaagcaacgc aatcttctcc atttttttgg
    18721 taccagtaga gccttttatc atttaaaaag gctaaaccgc tatccgaaga accaccataa
    18781 gcttgaagta gttttttata acgatccaca ttgaacacat cgccaatctg acaagatttt
    18841 ccttgtaaaa tcttaaccaa acataaataa acgagagtaa gaatcaacgt agccaatgct
    18901 atcggctccc atgctatgat ataatgcagg cgttcaatac taccacctgt aatatgagcc
    18961 ctgataggga atagtagtcc tgcaatataa aatagaactc ccattaaact aacgataatg
    19021 actccatcct taatacgctc ttcccagcta taaataaatt gttttttata gagagttggc
    19081 ttaatgacta ataagcctaa caatagtaaa ataaaccaga aagatagtcg ccaactaata
    19141 tgacctaaat taagataaaa caatgtcaaa ataatccaga taatagcaat tggaaaagca
    19201 tttttcactt tttgatcaat agttctagct aagagaataa aacaaacccc cagcaataaa
    19261 cctggaaact gccataacat ttgttcttgt aatgggtcca agcctagctt ctgcaaccac
    19321 ataatataag taatattttc aaaaaatgct gttgaaaata ttaagaatgc acctaaaata
    19381 cgcatcaaat ggctcaccat ggtttgtaga acagttgata ctaactcttt cgggacattt
    19441 tcataacgtt gatttatttg actacctaaa taatggataa agaaatagat acctgcaaag
    19501 aatggaataa tatagtaggc taaacgataa agtaataacc atgcaaccac agtttcttta
    19561 ggtagtccct cggcagcaaa ccctgtaaat agaactaatt caaaacttcc taatccaccg
    19621 ggaataaggg atacaatccc cacggcacaa ccaatacaaa atagtggtaa aattttataa
    19681 actggtagat gaatgcccat taaataaccg ataattataa atgctgcggc tgcacatccc
    19741 cattcaaaaa aagaaacaac acctaatttt atacgagtac tagatggcat atctccgaaa
    19801 taatggcttc ctttatgacc agaaatccaa taaataacag gaaaatacat actagcacca
    19861 attaatacca aataatagta atcaacatta gctttggcat gaaaaatatg gctcatgatt
    19921 aaggcaatca cactaataaa tgacagacca gaaataagat agggtaaaaa acgagtcact
    19981 tctcttagat cactcttctc ttgacctttt ttaccataaa aagccatgcg caacccaata
    20041 tcgattaagc caccgaatcc tgacaagtta ttaaaagtgt tgatacacca actagtctgt
    20101 aaaatatacc ttttgctttt atctgtacgt aaaagtccat tcagtacaaa gtcatatccg
    20161 gttgtcggta agacagcgat aacccccaca aggataatta agaaaagatt cattggagat
    20221 aattgcccaa aaacactctt tagttgctca acagaaatag ttcgtttcaa atgaatcatt
    20281 tcaacaataa taagcactaa aacagatata aaaaatacaa ttttaatcac agaagtcagt
    20341 gatttgactt tttcaattag ctttttcaac aatttctcct ttcaacgtat cgttgataaa
    20401 cttttttgca ataatattaa aaacatggga atcttgctta ataatgtgat gcccaaagcc
    20461 aactaaagaa taaaactccc cccttggaaa ataagaagca agtttcttag aatgatttaa
    20521 cttaattatg tccttatttc caaccaaaac cattacagga gttgacacat gctgtaaatc
    20581 agctggacta atcttcaaat cctccaacat aagcgaaata acttgagctt tttgcctcat
    20641 atacggaaag agtttcccta aatagtgaag gaatttatag gcaatcctta ctaaaagaat
    20701 atcccaccat cgctgaccat gaatagtcag gttccctgaa ttaagcaaaa gccctctaac
    20761 catatctgga aacatcgttt gaaaaactaa agctaaatta gcaccatcgc tatggcctac
    20821 caatataact ttatcaatct ctaaatgaac taagatatcc tttaagtcaa ctgctatttg
    20881 cctgaaacta atggtattta gctttgcatg acttttgcca tgccctctac tatccataac
    20941 aataacttgg taatacttag aaaaatatgc tatttgctta tcaaaatagc gactacttaa
    21001 gctattgcca tgtaaaaaaa caatcggttg accttgacca acgacacgat aataaatatc
    21061 tgaaccatct ttagtatgta accagttctc agtcataacc aaagtcacct ctctttgaat
    21121 tcgtagttat attatactat ttttaaagac aaattactta aactacctgt attatgagaa
    21181 aaaaatgatt actaaaagcc gttcaactaa atcgaacggc taagtagtat taaaataaga
    21241 atgttaaaag agctaatata ggtaaaccac cctgttttaa taagattttt ttatcaactg
    21301 tcaaagcacc atagacagca actagcaaca ca
//
LOCUS       DATRSD010000036        21132 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRSD010000036 DATRSD010000000
VERSION     DATRSD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21132
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.036"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(65..580)
                     /locus_tag="VBD85_001048"
     CDS             complement(65..580)
                     /locus_tag="VBD85_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO7133609.1"
                     /translation="MLKRLLTEDGELTKISRRFVWMLVIIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWTYR
                     NIKGWLLTIRK"
     gene            complement(580..1686)
                     /gene="rlmN"
                     /locus_tag="VBD85_001049"
     CDS             complement(580..1686)
                     /gene="rlmN"
                     /locus_tag="VBD85_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO7133610.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNDLRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGQ"
     gene            complement(1737..2315)
                     /locus_tag="VBD85_001050"
     CDS             complement(1737..2315)
                     /locus_tag="VBD85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO7133611.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KETKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            complement(2385..3719)
                     /locus_tag="VBD85_001051"
     CDS             complement(2385..3719)
                     /locus_tag="VBD85_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7133612.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHDNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANIQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNTIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPHGWMIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELINGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTCDTKQLTGYPSDSDWAKKIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFRKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGDVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            complement(3794..4867)
                     /locus_tag="VBD85_001052"
     CDS             complement(3794..4867)
                     /locus_tag="VBD85_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO7133613.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVFYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESVYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            complement(4857..5342)
                     /gene="coaD"
                     /locus_tag="VBD85_001053"
     CDS             complement(4857..5342)
                     /gene="coaD"
                     /locus_tag="VBD85_001053"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO7133614.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFYNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADDIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVVREVEKMSE
                     E"
     gene            complement(5339..5629)
                     /locus_tag="VBD85_001054"
     CDS             complement(5339..5629)
                     /locus_tag="VBD85_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133615.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            complement(5641..6345)
                     /gene="rsmD"
                     /locus_tag="VBD85_001055"
     CDS             complement(5641..6345)
                     /gene="rsmD"
                     /locus_tag="VBD85_001055"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO7133616.1"
                     /translation="MAIKLNNNFLIIMGTSFLLLLAYHIKKEGKEIPSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKMEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            6311..6760
                     /locus_tag="VBD85_001056"
     CDS             6311..6760
                     /locus_tag="VBD85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7133617.1"
                     /translation="MIKKLLLSLIAILCLTTLKSNLSSADTMTISPQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGE
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            complement(6805..7797)
                     /locus_tag="VBD85_001057"
     CDS             complement(6805..7797)
                     /locus_tag="VBD85_001057"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO7133618.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVRDTFENIL"
     gene            complement(7910..8974)
                     /locus_tag="VBD85_001058"
     CDS             complement(7910..8974)
                     /locus_tag="VBD85_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO7133619.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIAKTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEDEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            complement(9135..10037)
                     /locus_tag="VBD85_001059"
     CDS             complement(9135..10037)
                     /locus_tag="VBD85_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7133620.1"
                     /translation="MFSSDFGFEIINVDDLSLQEERFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNINLVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            complement(10198..11157)
                     /locus_tag="VBD85_001060"
     CDS             complement(10198..11157)
                     /locus_tag="VBD85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7133621.1"
                     /translation="MSKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFALAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSAQTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEDLYRSWGK"
     repeat_region   11294..11985
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11358..11389
                     /rpt_unit_seq="atttcaatccacgcacccgcaaagggtgcgac"
     gene            complement(12132..12425)
                     /gene="cas2"
                     /locus_tag="VBD85_001061"
     CDS             complement(12132..12425)
                     /gene="cas2"
                     /locus_tag="VBD85_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7133622.1"
                     /translation="MMVLVTYDVNTKTVAGRRRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVEIKNELLTIIDQKSDSIRFYLLGKNWQNRVETIGKNDSYDPDIGVLLL"
     gene            complement(12436..13461)
                     /gene="cas1c"
                     /locus_tag="VBD85_001062"
     CDS             complement(12436..13461)
                     /gene="cas1c"
                     /locus_tag="VBD85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7133623.1"
                     /translation="MKKLLNTLYLTQDNFYLTRERDNIVIKQEGKIVHRFPYRIIDGI
                     ICFSYLGASPSLIELCTEKQINLSFHTPQGRFCGRFVGSTNGNVLLRREHYRLADHAL
                     SIEYAKRFILAKVSNSRKYLIRFKRDHRDRIDIQLFEDINTELMWALELIQSAPDKET
                     LLGIEGQAANQYFRAFNELVLTDKDTFRFNGRTRRPPQDCINALMSLGYSLLTYECQS
                     ALEAVGLDSYVGFYHTDRPGRASLALDLVEEFRSYIVDRFVFSLVNKGQLNKRHFDIK
                     ENSSVILTEKGRTIFIESWQKRKHTVVEHPFTKEKVKLMLLPYVQAQLLSKAIRGDLE
                     SYPPFLI"
     gene            complement(13458..14129)
                     /gene="cas4"
                     /locus_tag="VBD85_001063"
     CDS             complement(13458..14129)
                     /gene="cas4"
                     /locus_tag="VBD85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEO7133624.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQI
                     LHTKADNPYIKGKRKDFLVSRAMHVSSKILGLYGILDVVEFYKDENGFSLKGKRGKWL
                     PCIVEYKRGKPKKDRRDIVQLVAQTICLEETFGCYIEKGCLYYHSVNQKKTIEITEEL
                     RQEVLDLAEQMHFYYDNKIIGKAEYFKNCPLCSLVDICKPRLSKKTRNVDNYIRQSLV
                     NEDSL"
     gene            complement(14131..14979)
                     /gene="cas7c"
                     /locus_tag="VBD85_001064"
     CDS             complement(14131..14979)
                     /gene="cas7c"
                     /locus_tag="VBD85_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEO7133625.1"
                     /translation="MLEHKIDFIVTFEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKPIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPISISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGIYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     REVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     KILEGL"
     gene            complement(14984..16879)
                     /gene="cas8c"
                     /locus_tag="VBD85_001065"
     CDS             complement(14984..16879)
                     /gene="cas8c"
                     /locus_tag="VBD85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEO7133626.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMTDKQMIIFPVTADSVARSGSNPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLMI
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDEDMPSVIAL
                     ATEENKRIGKSFIKGQKLFANDATYYIAILNKTSNGRIALKYFRQIQASQLLTNLDKW
                     QEKYSWESRSKSGKSRLRTPAFHDILNVSYGVDRDRFLELDNDNFKSDQVQKLVTSLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLNHTNQNRS
                     YLFGRLLAIFELIETLRYSLEGNGSDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     TLKLNRRGSWKKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYPKQNT
                     EVTESEE"
     gene            complement(16879..17607)
                     /gene="cas5c"
                     /locus_tag="VBD85_001066"
     CDS             complement(16879..17607)
                     /gene="cas5c"
                     /locus_tag="VBD85_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEO7133627.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERASYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFI
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRESVGLVDDISQEEYEAAVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMENGVITFKTQSDCDIVNTLSSYAF
                     KVPGEIKSVNDEYMEYDAMEKGEN"
     gene            complement(17740..20163)
                     /locus_tag="VBD85_001067"
     CDS             complement(17740..20163)
                     /locus_tag="VBD85_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEO7133628.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHLWHVACFSRQEASIIGQ
                     GDVLFLISLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKD
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLEISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVYLTDVINLILGE
                     ENSVLAIFNTKKTVHNCYTMLKDMTDRPIYQLSTNMCAQHRLDLVAKIKTELQNNTPI
                     ICISTQLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEEN
                     ISRLTEIKAKKEATESILYKIGSPINISTLNRDFFEYYYANNQGLMDYPLEDNISIYD
                     YLSLNTYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGDAEKKLAYLEELGT
                     SHFLSAEDYQTIKSLLKELQPFTVNVRENDPLLEATKSYLNGQILVLTSEYYDTERGV
                     KYDSASFYF"
BASE COUNT         6747 a         4268 c         3217 g         6900 t
ORIGIN      
        1 caactaaagt gattcttttt aaatagtaaa aggcttgaac aatgtcatag cctttacatt
       61 attttcattt tctaatagtt agaagccaac cttttatgtt tcgataagtc catagggcga
      121 aaggaccgcc taaggtattt gtccatagat cgtcgatttc aaaaacccga ttagcatcta
      181 ttaaaatatc taaaacaact tgagtacact ctatgaaaag agacatcaag aaagcaagta
      241 atataacgct tttatatttc cgtaaacttg gctttaggga tagtaaccct ataatgagag
      301 gaaacagcag taaaatattc actacatttt gaccaataac ccaaaaaatc tctttaaagc
      361 tagttagctg atctaaacta cgaaaagaat taaatggtac taaaagagct acaattcttc
      421 cgaagcgctg aacattcgga gttgataccc cctcaatcat aatttgaggc ccaaaacaca
      481 tcctgacaat aataagacaa tagataatca ctaacatcca aacgaaacga cgactaatct
      541 ttgtcaattc cccatcttca gtaagtaatc ttttaagcat tactgcccta ccttcgcttt
      601 ttgacgatcg cgtttcattg tgtttgaacg taattgtccg caagctgcat caatgtccgt
      661 accatgttct tgacgaacaa cacaattaac tccatttttc ttcaaaacgt catagaaagc
      721 ttctacacgc tctttaggac tacgactata ctggtcatgt tctgagacag gattgtaagg
      781 aattaaattg acataagata atttgcgaat ttttttcgtt aaatctgcta actcttgagc
      841 attttctggg gtatcgttga caccatttaa cataatatac tcaaatgtta cacgacggtt
      901 agtcgtctca atataatatt caatagccgc aaacaacttt tcaagaggga aagaacgatt
      961 aatacgcatg atacttgatc gtaaatcatt atttggagca tgaagagata ctgctaagtt
     1021 aacttgtaca ccttcatttg caaattcacg aattttatgt gctaaacctg aagtagacac
     1081 tgtaatgtga cgtgcaccaa tagctaatcc attatcatcg ttaactgtac gcaagaattt
     1141 taatacattg gtatagttat caaagggttc acctattccc atgaccacaa tgtgactaac
     1201 gcgctcacct tgtccacgtt catcaaaata tttttgaaca agcataattt gagcagtgat
     1261 ttcgccattg ttaagatcac gttgtttctt tatcaaacca cttgcacaga aagtgcaacc
     1321 aatattacaa ccaacttgag tcgttacaca tactgataaa ccataatgtt gacgcatcaa
     1381 aaccgtctca atcagcatgc catcaggtaa ttcaaaaagg tatttaacag ttccatcagc
     1441 agattcttga acaatacgtt gttttagagg attaacaaca aaattctcat tcaaaagtgc
     1501 aataaaatct ttggaaatgt tagtcatttc atcgaagctt tgcacacgct ttttatagag
     1561 ccagtcccag atttgtgaag cacggaattt cttttctcca tgttcaatag cccaagctat
     1621 tagttcatcg cgagttaagc tataaataga gggtttatag tcgggtgtat cagttttttt
     1681 aggcataata ttttctttct tcatggtgga ataaacggtt acagtttatc cctttcttat
     1741 tttttcttac gaataacaaa atgctgcttt gcagaggtca tttccttatc ttgctcttct
     1801 ttaatatgac gttcttgacg gcgttttgtc tctttacgct ttttacttgt aaaatgagca
     1861 ttggcatttg actgggaacg attatttcta gtttgtgact ttgatgattg tgatttggat
     1921 cttgacttac gacgcttatg aggtctttca tcatcaaatt taatatctct aggatttgga
     1981 ttctccaaca caaaataagc acaaccaaag ttacaatact ctttaatata atcttctaaa
     2041 cgtgaaatcc ggctattttt tctaatcgta ctagcatctt tgtaaaagcc tcttagcctc
     2101 aactgctcgt taccccaatc gccaacgata aaatcatatt ttaataaaat ctcagaaaaa
     2161 cgttgtccaa agacagtcac atcaaaagct gatttttcat taataacaag ttgaaattca
     2221 atatcatcac ttttaacgat attttcaaag tgaatgaact gtgggccagg atacttatta
     2281 tagttaagca tctctggtgt cacttctttt cgcataatac tcctttctct aaaatcattg
     2341 agaagaaacg actagcctct actctttatt tatctatttt tcatttatag tgtcatattt
     2401 attaaataat ctgctaccac atctctgagt aattcaggaa aaagcaagtc tacttttttc
     2461 gtttttaaac actcaaaata cgatgcaaaa taatactgat caacgattac aagaatcact
     2521 tcttctttgg catcaatatc tttctcatta tacactattt ttccattttt gtaatcaaat
     2581 cccgaatgta aaacgtctcc aaagtacttg ccacgaaagc ccataccatg aatcttttgt
     2641 tgcgctaaaa attgaccttg ttgataaata gtttcataaa tctctaaaag ttcttgacta
     2701 ctcactgtca atttagcgag acgcatttga tgaggtaatg attcgtggag atttttcctc
     2761 gaaaagtctt ttctaaatgg ctgaacaatt aagccagaat tgataatagc aacatctgca
     2821 cctgcataaa acagcatcgc atccatgact agttgacacg attcttctaa tgataatggt
     2881 gctggaaagg atattaaact ttcttttcta agctgttctt taccttctga ctcaatcttc
     2941 ttagcccagt cactgtccga aggataacca gttagttgtt ttgtatcaca tgttgagatt
     3001 aaaatatctt ttagtgtatg attatcaaaa gttatatcaa tactcccaac aaagcgacca
     3061 tatttaccag ctgctgcaag atatgtaccg ttaattagct ccccttcctc aaataaatgg
     3121 tgtgtatgtg cacctataat aaggtcaatt tctgaaaatt cttgagcaat ccttgtatca
     3181 aaacgaatac ctaaatgact catcaaaata cgacaatttg cctcttttat ctcatttatt
     3241 tgtaaatggc atttaagaca gtcaattgga tcttctatca tccacccatg aggttcatag
     3301 gttttataat aaggaaaagt ataagccaaa aaggcaagct tagttccttg ctgtgtctca
     3361 taaataatgt aaggttttgc ccaactcggt tcgattgtgt tatcttttag attgccaaca
     3421 ataaccgtaa aatctgaatc tttgtagact tggtccaagt cttttttgga taaaccaact
     3481 ccttcattat taccaatagt agcaagctca atacccagtt cattcatcaa ttggatatta
     3541 gccttgcccg agcttgcttc tgtcaaagga tggcttttat caatattatc ccccaagtca
     3601 agtgaaatcg tctctattgg ctgcgcttga ttatcatgaa aaaatcgttt taccttagga
     3661 aaattctcaa aatgagagtg cagatcattt aaatgtagta ttcttataag ctctgtcata
     3721 ctatcatttt agcactttga tagtcaaatg tcgcaaaaca aaaaagccga gcagacgccc
     3781 gaactctttg agtttatttt gtttttttca aataatcaat agcttgctga acattttgaa
     3841 ctggtacaat tttcatcttc gtacctaatc gcttagctgc tgcttttgcc tcttgataat
     3901 tcgtctgaac ctttgttttg ccttttttag catttttatc gatcggatta ttaggaacaa
     3961 agaaaatatc cattcctttt ttagccgctg aaacaacttt caaacctgct ccaccaatat
     4021 cgccaacatg cccattttgc tcaatagtac cagtgcctgc aatcttacgt cctttacgta
     4081 aatcttcttt aactaattgg tcgtaaatcg ctaaagtaaa cattaaacct gcacttggtc
     4141 ctccaacacc ttcagtatta aagtccacag gaacatcaga tgatacttct gtatggtctg
     4201 tcaaaccgat accaatacca tttttaccgt ttgatagttt aataacttta ccaactgatt
     4261 ctttcttttt accttgactt gtgtattgta cctttacttt atcacctaga tgaagagctg
     4321 caacatattt gataagttga gaagaatttt tgaaactttt gccattaact cctgtgactg
     4381 tatcagctaa atgtaatcta tctttaaatg tcgaattttt agctaagtta agcacataaa
     4441 ctcctttata agtaagagat acttgcttat tagctaactt taaagcttga tagacggact
     4501 cattttgaga agtctccatg tagaattgat taattctgag ataatcgtca ttactaaagc
     4561 cacctgttgt ctcctcttta ctagataatt ctgtaaaagg agttaaccaa gcataaaaaa
     4621 cctgagctgg cgttgcttga ctaacagata ccgctacaaa attgtacgaa cccttagctt
     4681 tatcagcctt tttattcact ttcaataccg aacggatatc ataagctcca cctggcattt
     4741 ccaaataata aggtagtctc accacaaggc ttgctaatac taacaataaa aaagcaaaac
     4801 ctataatcca ccatttaagc agacctagta aacttttatg ttttcttttt ggatctttat
     4861 tcttcactca ttttctccac ttctctaaca acactctttg gaacaaaagg cttcacagaa
     4921 gccttaaaat gtatcaattc acggatgcga ctagatgata tcggactcaa agatggcgac
     4981 gttgataaat aaactgtctc aatatcatct gccaactgtt tattaaaaaa ctccaaattg
     5041 gcttcataat caaaatcctg gctattacgc agacctcgaa caaaatattt tgctcccact
     5101 tcccttgcta agtcaacagc tagtctatct tgcgccacta aaactgaaac attcttaaac
     5161 tgacggatag cttcttctaa cattttttta cggcactcaa tcgaaaagta accttgcttc
     5221 tcaaggttat aaaataaccc aatgtaaaca tgatcaaata gatagctggc gcgctcaata
     5281 atatctaaat gtccattagt aacaggatca aatgaacctg taaataaagc tttctttgtc
     5341 attcgtcgtc atctccacaa taatacaacc ataaaaagac taccacagga atcaggcgaa
     5401 caatgtctat tgcctcaaaa ccgttagcta tccaattgat aagatttggc aaaaaagtaa
     5461 tcattgccat taatagatac aatggatttt ttgttcttga taatgaacaa actccttcat
     5521 catttttgaa gtgataatgt tttagcaact cttctaaata gtgaaggcgc aacaacaaat
     5581 aaatcaatat gactaagatg agaataatca tcactaactt gtaatccata ccaaagcctc
     5641 ctaacgaacg tagaccgtta ctttagaaat gccataaatt ttctgtttcc atatcccaaa
     5701 agaagcaatt tcttcaggta aatcaactga tttgtccgtt tcacaaacaa tcatgatatc
     5761 atcgcctaat aaacccttgc tatccatgat ttggatttgc tttacaattt cttccttagc
     5821 atatggcggg tccaacaaga ccaaatcaaa ttgtcccgtt aattgttcta aagcacggtt
     5881 ggcttccatt tttaataatt gaaattgctc cggactctta gtcattgcaa tattttcttg
     5941 aataacgacc tgcgcacgcc tatctttttc aactaggaca gcttggtcca ttccccttga
     6001 gatcgcctca atagctaagc taccactgcc agaaaaaagg tctaaaacac gaccaccttc
     6061 aaaaaaagga ccaatcatat taaaaatagc gcctttaacc ttatcagttg ttggacgagt
     6121 tgtttttcca tcaagcgttt ttaaaggacg ccctccaaat gttcctgcta ctactctcat
     6181 aaccactatt ataacaaaaa ataaggtaga gttatcaaaa aaattgctta taacaaacag
     6241 agaagatggt atctcttttc cttctttttt gatatgataa gctagcagta agagaaacga
     6301 ggttcccata atgattaaaa aattattatt aagtttaata gccatattat gccttacaac
     6361 gctaaaatca aacttaagta gcgctgacac catgactatt tctccacaac aataccaaca
     6421 tatgataaca aatagtgact taatcgccat cacagataag gcgttagtaa gcaaaagtca
     6481 acaatctgaa gcgactattt ttataggacg tccaacttgc caatattgta gagctttctt
     6541 accaaaatta ttaaaatctc aagccacact tcattcaaaa atttactacc ttgatagtca
     6601 aaaatacaaa ggggaacgat tgaagagttt tttcaaaaag catcatatta ccactgttcc
     6661 aaatttagca cattatcaac gaggaaaaat gactaaatat cttgttcaag gaagccaagc
     6721 tacacctcaa caaatccaaa ctttcttaag ccaaaaataa acttattgtt aaaatcagcc
     6781 aaacaaaact gcttggctga ttttttataa tatattttcg aaggtatctc tgacttcttt
     6841 tggccagaca cttgactgta cttctccaat atgtttttta cgaagcaaga acatagctaa
     6901 tcgtgattga ccaattcccc caccaatagt caaagggaaa aaaccacgta atagcgtctt
     6961 atgccactca aactctaaac gatcttcatc accggtgaga acaacttgac gtttaagagc
     7021 atcttcatca actcgaattc ccattgatga tagttcaaag gcagttccaa gctgttcgtt
     7081 ccatactaaa atatcaccat tgagaccttt aaaaccattc tctgatggtg ttgtccagtc
     7141 atcatagtcc ggtgcacgac catcatgtgg tttaccatct gctaactctc caccaatacc
     7201 aatcaaaaag actgcgccat actccttagc gatggcgttt tcacgctctt tagggcttaa
     7261 gtctgggtat ttttctacca attcttctgt atgaataaag gtaatacgct ttggtaaaat
     7321 ggattcaata tcaaaacgag cttccacggc aagctctgtt aggcggatag ctttgtagat
     7381 tttttccaca gtttctttta ggtagtccaa attacgtcta ccatcaggaa tgaccttctc
     7441 ccaatcccac tgatctacat aaactgagtg tgttggatca agagaatctt catctggacg
     7501 aagagctttc atatgaacga aaagaccttc tccctcatta aacccaaatc ttgccaaagt
     7561 atgacgtttc catttagcca atgagtgaac gacttcaaat tcagcttcag gtatattaag
     7621 gactttcacc gatacaggat gttcaatacc agataaatta tcttgcatac catccccgac
     7681 ctgactaaga ataggtcctt gcacttcgac gatttctaac ttatcaatca aatattgtgt
     7741 aaaagtattt ttgacaaaag aaatctcctg ttgctgatga ataaagcttt tcttcatatg
     7801 ttcctcctta aattataaaa tgttagagct attatactcc ttcaagtaac aaaatggaag
     7861 aggagttgta gaattattct gactttttta caagtttttc tgaaaatctt tactctagaa
     7921 ttgtttttgc catgacttgt cgcaattcct tggtcattcg ccgtggtcct ctcacagtct
     7981 taacgccgtg ttctgctaaa aatgctacag gataggtaga atcttcaaat tgccttatca
     8041 gtttgagtcg catataagct ttgacatctc ttgctcctaa gtaagtactt gttttaaggt
     8101 cacgttctat tacttgacag acatagcgca aagcacgaat aggtgctgta atataaataa
     8161 acacataatc tcccaccgca atctttcctt tttgagtcca atcaatcaat tgatgctctg
     8221 caaactcctt atcaatatcg taaaatttag gattagcagg aataatccaa aactctggct
     8281 cagaatttga ttttttattg ggagaaacaa gcttacgact atctgatacc aatttaaaaa
     8341 tttctatatc agacaaagta ttgtctaaaa cgatagatac ccaagtcttt ttagacatat
     8401 gataagaagg ataaatacct tctttttgta agagtatttc catatcttga ggattgactt
     8461 taatattcaa aacttcaacc tcatcttctg acagttgagc tggaacattt tctagtttcc
     8521 ccatcttcaa tggaaaaagc agagcatacc atttacctga tacacgataa gaagcatatt
     8581 gtgggtgctt cgcaaatgga taatcaactg aatcttcaaa agttttagct atcttctccg
     8641 ctaaacgatt actttgatct ttggtaaata ataagtcctt acaacaagaa tctgaaatat
     8701 cacctaatat agccatataa gccgatcgta cttcgccaac aaacgaacca ttataatttg
     8761 ctgctcgaag cggtaaatat tcttcttcaa gtgccgaatc aataacctta ccagctatct
     8821 ttccatccaa tgaaatcgtg ataatagctt taaagtcatt atccataaat gtttgacaat
     8881 agatatactc ttggtcagat ttgataaatc caaactcctc aagcgatgaa aaaataaaac
     8941 gttttttccg gaaaaaatct gattctattg acatagtaac ctctctattc actgtacatt
     9001 atcctctatt atgtcgtttt aagagctaga aagcaagctc aaagtcaaag atgaattcta
     9061 gtttctttgt acaaaaaaaa gcatgttgcc atgcttaaaa ctaaactaat tgatagcacc
     9121 ctcttattaa ttctctattt atgtttggga tgattatcct ctttatggac ataaatgtac
     9181 ataacaatag agagcataat agtaatgata gtcgtaatta aaataatcaa tacccagaca
     9241 gcatcaacac tgtcaaaagg caacttaaca ttcattccat aaaagctggt aaccatagca
     9301 ataatggaaa taccaatgga aataattgtt aaggtagtta cattgtcgtt aaggttattg
     9361 ctgaggacat tattataaga agaagaaagt tcttggaaaa cctgtgaatt taaggaacac
     9421 atgttagata attgtcttgc ttcaataata gcatcttgaa gttgcatctt ttcatttcta
     9481 gtgttctctt tatcttgctc aatgttttgt aaatcggtca gcatttcaaa attttgctta
     9541 gttcccatca ttaagtgtac tgttccactt tgtaaatttg ccagccttgc caagttcgat
     9601 ttgtttggtc ttttacgcaa atcattgatt agtttatctc tttcttcaga aatagtatct
     9661 aaaacatcaa aatattgctt tgagaaagca gtcactaatg ataataagag gtgtttcgga
     9721 gaatctacct tttcaacacg attaagtgct ttttttacaa ggttaatatt tttatgatta
     9781 gctaatacaa acaagccatc ttctttcaaa aatagagtca ctggtatcac tctcggcaca
     9841 tgacgaacat catcaacgtg accttgcgta tccaaaagct ggtaaacaag tgtcaaatgc
     9901 ccctcaatat cacgtacgaa agaagactca ttttcatcac gtgcataagc cagcaattct
     9961 gaaggtaaaa atctctcctc ttgcaaggat aggtcgtcaa cattaattat ttcaaatcca
    10021 aaatcgctcg aaaacataga agcacctcac tctaaacttg tctacattat gaaacaaaaa
    10081 tcgagaaaaa acaagtagaa aaccctttta aatctaatta accttatgag agcaaaacaa
    10141 aaaaggacta ctaaaataac atctagtact ccttattaat atgctagata gccagcatca
    10201 tttcccccat gaacggtata agtcttctat aatcttaaca cctgacagcg tcaactgagg
    10261 cgtcatgata tgacttaccc tttgcccttc taaaatcatt tgactaacat gataagcttc
    10321 tttctcaaaa tcactaacca tgtcaacttg aatcgtttgc gcactagtat catttctcac
    10381 caattttgca tgagtggttt tccaaaaatg tggaatgatt agacgacctt cagttccata
    10441 aataatcatt tcatgcggta aatttaaacg tgttgtcaag aagatatcta cgaggacccc
    10501 atttgataat tgcaagagta gtttggattg actatcgctt tgaccttttg gaaaagtcgc
    10561 agtcccacta gcatgagtta tagtcgcatc aaatagatat tgtaaataag acagggcgta
    10621 gggcgccata aaatgcactg ttcctccacc taactcaagt tctctaaacc atgttacatg
    10681 gtcaatgttt gggtaggctg ttgtagatga gatggagatc acttctccta tttcaccaga
    10741 ggcaagtaat tttttaatca cttgtgtcat cggaataaat accgattttt gtgcctccat
    10801 caaaaacaga ttacaacttt ctgccaaagc gaataattca ttcgcttggt cgtaagtcaa
    10861 ggtgaaaggc ttttctacca acacatgttt tcccgctaac agagctgctt tagcaacttt
    10921 ataatggtct tgattaattg ttgccacata aatcacatca attgactcat ctgctaacat
    10981 atcctctaac ttatcgtaag cctttgggag atgatatttg ttggcaaaag cttgcgcaga
    11041 ctcaagagtt cgactcgata cagccacaac ttcaccattt cctgctaagc ggactccttc
    11101 aataaaacga ggcgccacct ttgccgttga cacaacacca taccttacct ttgacataaa
    11161 aactcctttg acattgatag agttattata gcataagaac tagcaattta tctttttagg
    11221 gattaataca atttaatcgc atctctgtta aaagttagac aattttaata gcataatttg
    11281 aaagatttaa aatagttgaa actacacacc cgcaaagggg cgatgataat gaattaataa
    11341 aaaaataatc attattaatt tcaatccacg cacccgcaaa gggtgcgacg cgttgatatg
    11401 aaaggaaatt atagagatat gatatttcaa tccacgcacc cgcaaagggt gcgacaaaac
    11461 ggatggttgt atattgcagg ctatcagcta atttcaatcc acgcacccgc aaagggtgcg
    11521 actgatctaa ttagtgatat gctacgtctt tacgacattt caatccacgc acccgcaaag
    11581 ggtgcgactt tggctgtttg agtagctcgt tgacttttgc ctatttcaat ccacgcaccc
    11641 gcaaagggtg cgacataatt atggtagtct tatttttaat ataaaataat ttcaatccac
    11701 gcacccgcaa agggtgcgac gtttgctctg cgtcaagcgt aacagcgaga cttaatttca
    11761 atccacgcac ccgcaaaggg tgcgacgtct tttcagacag aacataaatg gaaatatagt
    11821 aatttcaatc cacgcacccg caaagggtgc gacttttccg ttttttcata gcaatatgtg
    11881 ggactatatt tcaatccacg cacccgcaaa gggtgcgact tttccgtttt ttcatagcaa
    11941 tatgtgggac tatatttcaa tccacgcacc cgcaaagggt gcgacagtgc gcgattgcac
    12001 cgtttataac taaaaccaag aatttattaa acaagttggt tcaataaaaa ctagattttg
    12061 actactattt tctttttttt agcgcgaata ctccgctttt tcatgtgtga cccgaattca
    12121 catataaatg tttatagaag taatacccct atatcaggat cataactatc atttttgcca
    12181 atagtttcca cacgattttg ccaattttta ccaagtaaat aaaatcggat actatctgat
    12241 ttctggtcga tgattgtcag caactcgttt tttatctcca caaactcagc tggagtcact
    12301 gaacactcaa aaacagaatt ttgtacacgt tgaccataat caacacaaag ttttgcgacg
    12361 tgacgaaggc gtctcctacc tgctacagtc ttcgtattga catcataggt tactaaaacc
    12421 atcataattt tactcctaga ttaaaaatgg tggataggat tccaaatcac cacgtattgc
    12481 tttagataat aactgtgctt gcacataagg cagtagcatc aacttaactt tctccttggt
    12541 gaaaggatgt tcaactactg tatgcttgcg cttttgccaa gactcaataa agattgtacg
    12601 tcctttttct gtcaaaataa cactactatt ttccttaata tcaaaatgtc ttttatttaa
    12661 ttgtccctta ttaaccagag aaaaaacaaa tcgatcaaca atataagaac gaaactcttc
    12721 taccaaatcc aaagctaaac ttgctcgacc aggtctgtcg gtatgataaa atccaacgta
    12781 actatctaat ccaactgctt ctaaagcaga ttgacattcg taggtcaaca gactataacc
    12841 caatgacatc agagcattga tacagtcttg cggtggccgt cgtgtccgac cgttaaaccg
    12901 aaatgtatcc ttatccgtta agacaagttc attaaaagca cgaaagtatt gatttgctgc
    12961 ttgtccttct attcctagca gagtttcctt atcaggtgca gattgaatta gttctaacgc
    13021 ccacatcaat tcagtattaa tgtcttcaaa caattgaata tcaattctat ctcgatgatc
    13081 acgtttaaaa cgaattaaat attttctaga attagatact tttgccaata taaagcgttt
    13141 ggcatattct attgaaagag catggtccgc taatcgataa tgttcacgtc taagcagaac
    13201 attcccattc gttgaaccta caaagcgacc acaaaaccga ccttgtggag tatggaaaga
    13261 aaggttaatt tgtttctctg tacaaagttc aattaaggat ggtgaagcac ctaaatatga
    13321 aaaacagata ataccatcaa ttatacgata tgggaagcga tgaacaattt tcccctcctg
    13381 cttaataacg atattatctc gctcccgtgt gagataaaaa ttgtcctgtg tgagatataa
    13441 agtatttaac aattttttca taaactatcc tcatttacca aagactgtct aatgtaattg
    13501 tctacgttac gtgttttctt gctcagccgt ggtttacaaa tatctaccaa agaacaaaga
    13561 ggacaatttt taaaatactc agctttacct atgattttat tatcataata aaaatgcatt
    13621 tgttcagcca aatccaaaac ttcttggcgt agttcctcag taatttcaat agttttcttt
    13681 tgattcacgc tgtgataata aagacagcct ttctcgatat agcacccaaa agtctcctct
    13741 aaacaaattg tctgtgctac tagttgaaca atatctcgtc tatctttttt cggtttacct
    13801 cgcttatatt caacaataca aggaagccac ttccctcgtt ttccctttaa agaaaagcca
    13861 ttttcatctt tataaaattc aacaacatca agaataccat atagaccgag aatttttgat
    13921 gacacgtgca tagcacgtga gaccaaaaaa tcttttcgct ttcctttaat gtaagggtta
    13981 tctgctttag tatgtagaat ctgcccatgc gctgtcgctt cattatcaag ccattgttgc
    14041 tcaatatgaa tcaatgccca ttgacgttta caaaattgga aatgttgaat acctgataac
    14101 atcaaataat catcttcggc atagaccata ttatagccct tctaggattt taaccgttaa
    14161 acctttcgct tcatatttag ctagtttttc ttgatttaga tgaatttgat aagcgtcata
    14221 agtgcttttt tcttctattg attgattata ctctaacaag tcaaagacac gcgcacttga
    14281 aacatttccc aatttgcttg aatgagtaaa ccaaaagact tcgcgaactc gcatagaacc
    14341 ttctggacgt gcagacgatg catcattttc aaataagcta accaaaactt ctttaatagc
    14401 ctcagcatct tcctgagaaa aaccagtctt ttcagcaaaa taagcattga tagaaccttt
    14461 aagcacataa ataccataat ctacaaaatg ttttgtcccc atagtatcag aagagcggcc
    14521 actattattc ttagcttcca taccattggt actacgtgta atttgaaggc tagaaatgac
    14581 aattggctcc aaggactttg ccatactgat tgaaatcggt ccgcgcaccc caattgattt
    14641 tttcagataa gtaaaaactt gtccaaaagc acgaacatca aaccataatg catttgcttt
    14701 ttcttcaatt tctttgtcag gtgtcttagc tgtaaaatgt tgcgaaaagc gtttttccag
    14761 tgaacgaaag tcgtcttcaa tacgctcatt agcttgcaca aaaataggtt tccccatatc
    14821 ttgcaaacga ttacgaatct tccgtttaat ggagacatca ctcattacac cgtatccttt
    14881 agcatctgta cgaggcatgt ttccgtttaa tggatcacca tttgcatttg cttctttcac
    14941 ttcaaaggtc actataaaat caattttgtg ttccaacatc tttttactcc tcactttctg
    15001 ttacttcagt attttgtttt ggatagtaat agtttttctc agcataatac ccaaaaataa
    15061 agcggtaatc taacggtttc tccattgttt ctgtttctaa tagaggattt aaaagttcta
    15121 aaatttcttc tttttctttt tctaatttct tccaactgcc acgacgattt aatttcaatg
    15181 tttcttcgta aggcttaatt ttattctcca ataacatcat caattttgtt ggctgtccag
    15241 tataggctgt ccaataacgt tcagcattag taatacgatc gctgccgttt ccctccaagc
    15301 tataacgcaa ggtctcaatt aattcaaaaa ttgctaataa tcgtccgaaa agataggaac
    15361 gattttgatt ggtatgattt agcatcggtg aaaactcctc cccattttgt ttgtgtaaaa
    15421 ttgctaagca gacctgctca acttgatacc agtgcttacg ataacgatgt cgttctttaa
    15481 cattgttacc taattttttg acaatagact gtggcatagg tttaccatca atcaaacttg
    15541 tcactaattt ttgaacttga tcacttttga agttatcatt gtctaattca aggaagcgat
    15601 ccctatcaac tccgtaggac acattaagga tgtcatgaaa ggcaggggtt cttaagcgac
    15661 tcttcccaga cttagatcgc gattcccaac tgtatttttc ctgccacttg tcaagattgg
    15721 tgagtaattg ggacgcttga atctgacgaa aatattttaa agcaattctc ccgttgctgg
    15781 ttttattcaa aatagcaatg taataagtgg catcgttagc aaataatttt tgtcccttga
    15841 tgaatgattt accaatcctt ttattttctt cagttgctaa tgctataaca gagggcatat
    15901 cctcatcatc atcttcttca agtccatcat caaagattgg tgatacgata tccaaccgac
    15961 tatcatttgt taaatcatca ctaaaccagt tgattaaata ttgagaagac cctaaccaag
    16021 tactggtatt tttattttct aaaaggtact tgatcattaa atgaatcttt tcggaagttt
    16081 catagccaac actaaaaaca tcttcacgtt ctctaaaacg tcctttataa gcttctcttt
    16141 tattactaac agagatgatt ttagcattcc ccatcaaacc tctatgctta tcggtaagct
    16201 gttcctctcg tccactaata ttacaaatac caagattttc acgattggct tcaacgaaag
    16261 aaatatagga ttggtgtaaa gttttaaatg tcgaaaccga ttcattttta aagccctgga
    16321 actgatcaat tgaaaattct aaaaagcaag ctgataaatc aattaatttt tcttttccag
    16381 tggcatcaca aaatgtgact tttaattgat tattttgata atcaggacca attaaagaat
    16441 cgagaatcag tgtcagaaat tctggtttca aaataaattg ctgaacaatg gttaaaaacc
    16501 tcttgacgtc accctcctca caataatcaa tccagttatt aagttgctta tgaaaagaat
    16561 catactggga ctgtcccatt tcagcactat aataagcaaa tttatcgact aggggatgcg
    16621 gcgcaggatt actacctgac ctagcaacag aatcagccgt tacaggaaaa ataatcattt
    16681 gcttatctgt cataaattct gccttgtgaa actggccatc tttgtcaagt ttgactgaaa
    16741 tgatattttt gccatttgac tttaaactcg tatgataaat tggcagtaaa accggctcat
    16801 tatttctttt ttggtgatca accaagtctg ctagctctgc tttttcataa gtcttcaaga
    16861 gagaagtaaa aaaatccatc agttttctcc tttctccatc gcatcatact ccatgtattc
    16921 atcgttaacc gattttattt cccctggcac tttaaaagca taactagaaa gtgtgttaac
    16981 aatatcgcaa tcagactgtg ttttaaaagt aatgactcca ttttccatca cagtctttgt
    17041 aaagtatgat ttcaatggtg tctttttgtc tttcggatag gcaaaggaat ggaacatgat
    17101 tcccaagtcg atattgacac cattataata cgacacagca gcctcatact cttcttggct
    17161 aatatcatct acaagaccta cactctctct tgtacccaaa aacacatcac gacgtccacc
    17221 tttacgaata gaacgctcca taatggcttc atgtttagct ggaagtctgt ctgagttcaa
    17281 atcttttcta tcttcattcc aaataaaatg aaatttgatt agataaacaa catcactcaa
    17341 ataggataca taacttaaat ctgcactata atcatgtaac agagccctga caccctgtaa
    17401 ttcggtttga atctggttaa taaccttaac ctctgtgaca atattagtaa aggtcggctt
    17461 ataatagatg gcatcaacta taccattcag cgcctgtcta gttggaaccg aataggatgc
    17521 acgttccgat cccccttttg tggctggatt tgtaaaaagg gctcgctgac cacttactct
    17581 cacgtagaag tctctagatc tgtacaagat attttcctcc ttttgttttt ttataatcta
    17641 attttattaa atagtctaaa ttgataataa ttattaatga tgattggcag gttaacaaga
    17701 tcctctacca accttttgtt aatctgcttt cgttttgagt tagaagtaaa agctagctga
    17761 atcgtattta actcctcttt ccgtgtcata atactccgac gtcaaaacca gaatctgacc
    17821 atttagataa gattttgttg cctcaaggag aggatcgttc tcacggacat taaccgtaaa
    17881 aggttgtaac tcttttagta atgattttat cgtttgataa tcttctgctg ataaaaaatg
    17941 tgacgtacct aattcttcta aataagccaa ttttttctca gcatcgccat aaggtacgag
    18001 aattcctatc atatcattat tgatgaggtt cattttggct cctgctgttt taaaagcttg
    18061 ttttaactta cctttgaact ttttatttgc cgtctgataa gtattaaggc ttaaatagtc
    18121 gtagattgat atgttgtctt ccaaaggata atccatcagt ccttgattat tggcataata
    18181 atactcaaaa aagtcacggt ttaaagttga gatattgatt ggagacccaa tcttataaag
    18241 aatagattct gtggcttctt ttttagcttt tatttctgtc agcctagaaa tattttcctc
    18301 ttcattggtc agattgacaa gagtgacctg acctttatct cgtttgcctt ctcgattaca
    18361 tcgtccagca gcctgaacaa tagaatcaat ccctgaatag gaacgaatga cacgatgaaa
    18421 gtcaacatct acacctgctt ctattaattg tgtgctaata caaataatag gggtattatt
    18481 ttgtaactcc gttttgatct tagcaaccaa gtcaagccta tgctgcgcac acatattcgt
    18541 cgaaagctga tagatcggtc tatcagtcat gtctttgagc atagtatagc agttatgaac
    18601 cgtttttttc gtgttaaaaa tagcaagaac tgagttttcc tctcctaaaa taagattaat
    18661 aacatcagtc aagtaaactt tctgatcact atcatcaaat tttctaagct ctgtccttga
    18721 aaaaatttgt ttttcttcaa tcgttaactc aactatttca gctaattctc ccaagttccc
    18781 tccataacag atacgatggt caatctctga agaatcataa gcaggttgtg ttgctgtaca
    18841 aagaacgatg gttgtatcca taactttatt taaaaaattc atcgttaaat taaacaaagt
    18901 ggtgacttca ataggcagtg actgaacttc atctagaatc acaacactat taatcaaact
    18961 tgaaaaacgt ctcaaattag ccgattttgt tttaaaaagc gtttggaaaa attgaaccat
    19021 agaagtcaag actacttgac tgtcccagct atcaataaga taagctgaca ataaactgtc
    19081 cttgtcatca tcttcattag cctgtttaac catattggaa tggtgttcta aaacgccaag
    19141 gtcacccgta acttttctaa tttcggaagc attttgctca agaaccgaaa gaaagggcgt
    19201 tatgtaaaaa aatcttgatt tgtcttgatg aactaattgg tgaaacgcat agcgcatact
    19261 aagattagtc ttgccagctc ctgtcggtaa atctaagcga tagatccctt tggaatcccg
    19321 tttacctctt tctttaacac gctcagcaat ttctgtccga atagtattca actgattgtt
    19381 tggtcgtcca aagctagcat atttttgttc aatggctgct aaataagaat gttttagaga
    19441 ggatcgctct gttttatcca taggacttat ttcaagacca taggcattta ctgtgtccaa
    19501 aatatcagcg ttttttaaga gcgacaagta aagtctcacc atacaagact gataataatc
    19561 ccactcacta ttatcttgcc agtttaagga agacatagct tgttggtaat tatcaaaagc
    19621 tttatcaata agctctctta aatctttgta gccgtaatca cacaattttt cttctaattt
    19681 tatagcatac cctttaatat cttcgtgata gtgataatca tctaagtctc tgttgatacg
    19741 gtttttaaat ttattaaagc tataatattc agcatcgtca gaacagtagc aaaaatcata
    19801 catcccatga tgagccgaga tgacataacc caccatttca ttgaatgtca ttctctcatc
    19861 tttatcaacc cctcggcttt ttagatgagg cccaataata gagcataagt attttgcccc
    19921 tgcataagaa tggtcaacat gtcgatttgg attatttaat aatttatctt gaaaggctcg
    19981 atcagctttg cctagatcgt ggtatagact aattaaaaaa agcacatctc cttgaccaat
    20041 tatagatgct tcctgtcgac taaaacaggc cacatgccat aagtgctcat ctaaagactg
    20101 ctttttttca gattcttgtt ctattttctt ttgataatgt gccaacgttt ccttagtcat
    20161 catcaacctc tctccctcat ccataactca tatccctcaa tgatatacta acatttttga
    20221 aaggaattgt aaacggatac aatagttaat acttccccca aaaagcctct aaatgacttt
    20281 ccaaaaaaac aggaatcttg acaattcaag atccttgtta ggagttctgt aaaaaaaatt
    20341 atttttcggc taggcgtagt ttaagttagg aaacttaaaa ctagcttaat tggaagatgt
    20401 taaattttta taacacctaa cgctgacaat gctgataata tcgaccttta catttggtac
    20461 ttattttttg tcatctcgac tagtaaacgt gtgttctaaa tcgccaagca ttagtttttt
    20521 cagcacatac aacttttcct ttcattcttg cggagatacc atcaaaatgc taaattaaat
    20581 ttaacattga caacaaaaca cttgttccct aaacagtatc aataagtcaa ccgatcggtt
    20641 taaaatacta ctgtcttatc gtatgtaacc ccctctatct agttgctggt ttcaagtagt
    20701 atgataaaac ctacatttta tgcatgctta ctaccattag aatggaattg ttatgaccaa
    20761 ggtaaagtat tcgttatttt ttctcatcta tcttgcacta ctatatctaa ctagtatcta
    20821 ctttcctgct atttataaag tcctcatgta tcttcacttt ctatgtctat tcatttttag
    20881 cattatgttc ctttataatc tgattgtcat tttaaaatat atctttacgc ataaaaagaa
    20941 gtagattagc atatattttt gtttgctcgt tataaaatta agtttttaag gaatgttaaa
    21001 actactaaac acacccaaat tgatagataa tagaaaatac tactaatatt atgaatcatt
    21061 acaacatctc tagaaaatca tgatgtgcta aatcaaaaga tgcatgtgtt tttacttgaa
    21121 aaaaattttt ta
//
LOCUS       DATRSD010000037        20789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRSD010000037 DATRSD010000000
VERSION     DATRSD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.037"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            106..579
                     /locus_tag="VBD85_001068"
     CDS             106..579
                     /locus_tag="VBD85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO7133629.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            601..1242
                     /locus_tag="VBD85_001069"
     CDS             601..1242
                     /locus_tag="VBD85_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7133630.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTPQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1278..2180)
                     /locus_tag="VBD85_001070"
     CDS             complement(1278..2180)
                     /locus_tag="VBD85_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7133631.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPLTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            2355..3845
                     /gene="lysS"
                     /locus_tag="VBD85_001071"
     CDS             2355..3845
                     /gene="lysS"
                     /locus_tag="VBD85_001071"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO7133632.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITGID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3920..4390)
                     /locus_tag="VBD85_001072"
     CDS             complement(3920..4390)
                     /locus_tag="VBD85_001072"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO7133633.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4405..5598)
                     /locus_tag="VBD85_001073"
     CDS             complement(4405..5598)
                     /locus_tag="VBD85_001073"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO7133634.1"
                     /translation="MAFSPIKKLLQDIKSGKMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFTAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5616..6266)
                     /locus_tag="VBD85_001074"
     CDS             complement(5616..6266)
                     /locus_tag="VBD85_001074"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO7133635.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDAPKLVPLLRKGDSVAVN
                     GVCLTVLDKSETAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVI
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6247..7356)
                     /gene="ribD"
                     /locus_tag="VBD85_001075"
     CDS             complement(6247..7356)
                     /gene="ribD"
                     /locus_tag="VBD85_001075"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO7133636.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALKAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7550..7664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8085..9425
                     /locus_tag="VBD85_001076"
     CDS             8085..9425
                     /locus_tag="VBD85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7133637.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFVIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANAKWNTYLAYLVAIILTLLNLKLIMDLL"
     gene            complement(9527..10324)
                     /locus_tag="VBD85_001077"
     CDS             complement(9527..10324)
                     /locus_tag="VBD85_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133638.1"
                     /translation="MIRHSFKSKVLLLVFILTIIVNYLSATGFLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSIQILSLIAAIILGVSFFIIGYYNVKERYKQKNTSPYSRVL"
     gene            complement(10448..10660)
                     /locus_tag="VBD85_001078"
     CDS             complement(10448..10660)
                     /locus_tag="VBD85_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO7133639.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(10787..12073)
                     /locus_tag="VBD85_001079"
     CDS             complement(10787..12073)
                     /locus_tag="VBD85_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7133640.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIDGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTVRA"
     gene            complement(12203..13129)
                     /locus_tag="VBD85_001080"
     CDS             complement(12203..13129)
                     /locus_tag="VBD85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7133641.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            13469..13744
                     /locus_tag="VBD85_001081"
     CDS             13469..13744
                     /locus_tag="VBD85_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO7133642.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            complement(13785..14216)
                     /locus_tag="VBD85_001082"
     CDS             complement(13785..14216)
                     /locus_tag="VBD85_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7133643.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(14213..14611)
                     /locus_tag="VBD85_001083"
     CDS             complement(14213..14611)
                     /locus_tag="VBD85_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO7133644.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            complement(14626..15399)
                     /locus_tag="VBD85_001084"
     CDS             complement(14626..15399)
                     /locus_tag="VBD85_001084"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO7133645.1"
                     /translation="MINPVAIRLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYVPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYRRREQHISYY"
     gene            complement(15392..16327)
                     /locus_tag="VBD85_001085"
     CDS             complement(15392..16327)
                     /locus_tag="VBD85_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO7133646.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            complement(16555..16818)
                     /locus_tag="VBD85_001086"
     CDS             complement(16555..16818)
                     /locus_tag="VBD85_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO7133647.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            complement(16899..17357)
                     /locus_tag="VBD85_001087"
     CDS             complement(16899..17357)
                     /locus_tag="VBD85_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO7133648.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYQHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            complement(17320..19482)
                     /locus_tag="VBD85_001088"
     CDS             complement(17320..19482)
                     /locus_tag="VBD85_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO7133649.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQNLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     QHPSKYLSVGDIVTVWVVEVDAERSRIGLSLIKPDGLD"
     gene            complement(19587..20630)
                     /locus_tag="VBD85_001089"
     CDS             complement(19587..20630)
                     /locus_tag="VBD85_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7133650.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLPIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGK
                     EVAPIVRKYFEKN"
BASE COUNT         6635 a         4188 c         3156 g         6810 t
ORIGIN      
        1 ctgaaatggt attcaagctt atttctaaga gtcagtagaa atttactata atcatcttta
       61 aatgttataa taaatatgaa acaaacttat tagttaaggg tcattatgag taaaaagaat
      121 aaaatcaaaa aaactttagt tgatcaaatt ttagataaag caaaaatcga acatgatagt
      181 ctacaattgg atgctttaca gggagattta cccaacggta ttcaaaaaca agatatcttt
      241 aagacattag cgctaatcgg tgataaaacc ggtcccataa taggtatctt acctctcacc
      301 gagcatttat ccgaaaaaaa gttagctaaa atttcaggaa acaaaaaggt acaaatgata
      361 cctcaaaaag atctccagaa aataaccggt tatattcacg gagcaaataa tccaattgga
      421 attcgccaaa aacataatta ccctattttt atcgatacta tcgctttaga aaaacaagaa
      481 ctcattgtct ctgcaggaga aattggacgt tctattcgta ttaatagtga ggttcttgca
      541 gatttcgtaa atgctaaatt tgccgatata aaggaataat aattaggatt ggagaaaaat
      601 atgaaatttt atcttgttag acacggaaaa acacaatgga atttagaggg gcgtttccaa
      661 ggtgctaacg gagactcacc tctccttgaa gaagctattg aagaattaga agaacttgga
      721 caatacctat catctattca ttttgatgct gtttattcta gcgacttagg tcgtgctaga
      781 gatactgtta atatcctcaa tgatgctaat tcatgcccaa aagaaatcca ctatactcct
      841 caacttcgtg aatgggcatt gggaacttta gaaggttgta aaattgcaac tatgcaagct
      901 atctatcctc gtcaaatgac cgcattttat caaaatccct tacaatttaa acatgacatg
      961 ttcggtgcag aatctcttta tcagacaacg catcgtgtag agagtttctt acgttctcta
     1021 gcttcaaaaa attacgacaa ggtccttatt gtaggacatg gagcaaacct aactgcgtct
     1081 attcgttcct tactgggtta tcaatatggt agtttacact acaaagataa acttgataat
     1141 gccagcctca caatcataga aactcatgat tttaaagact tcaactgtct gacttggaac
     1201 gataaatctt atttaaggca agaggtcaaa atgacacact aaaataatcc actagattca
     1261 tctagtggat tatttaatta tctagcatac ttcatgcgta atactttttc ttgttttgca
     1321 gttaggcgca gtaaaataac aactttgtca acgctcatat tactctcaag cagacgctct
     1381 gctgccatca ttaaaccatt attaagtccc atttcaagga ctggattact tttatcgttg
     1441 atatcaaaaa taaatttatt atcaggtaag tcaaacaaca ctttaacagc accgaataat
     1501 tgctcaaagt tatcaatagc aacatcataa actggtttaa ttcccggttt taaagaacgt
     1561 cctctatggt tgaaccacat agaatgccaa ccaccattaa atgcacccat aatatcatta
     1621 tcatatgaat cacccacata aagtgtagtt aaagggttca tatcaaattg ctctgctgct
     1681 aaattaaaaa tctccttttc aggcttttga aaaccagttg cttgactaac tataacacgt
     1741 tttggatcaa catagtcata aagtcccaat tttttaactt ttttcaattg atgttccgta
     1801 ggaccatttg taataattcc cattggtaca ttttttgatt tcaaaaagtc aagtgtcata
     1861 cgcatctcat caagcattgt aatattttca agttcatgtt cgtatatttc ttgaaaataa
     1921 ataccagtag cttcgtcaat ttcacgataa ccaaactcta aaagcgtttc tttgcatcgc
     1981 cagaaacgaa aatattctgt tgtccattca ccagccatta ctcgtggaaa gccaatatct
     2041 gaataatgac gaaagcgaat ataagcttgg ttaatagcgc tcatatcaaa atcaggaaag
     2101 catttctcca cagcaatacg gtaaggtgct tgttgatcgt aaatcgtatc atcaacatca
     2161 aaaacaattg aagtaatcat gattatcctt ttctttctga attttcaatt gttaattata
     2221 cctttttcta cccttatttt caaatggtga aaagtctatt aaaaatgaga ataacaccag
     2281 aaactcataa aataaattat gaattatcag tgtttttgtg gtacaatagg atgaatacta
     2341 acttggagga aattatgtct aaccaacata ttgaagaatt aaatgaccaa cagattgttc
     2401 gtcgtgaaaa aatggctgct ttgactgaac aaggcattga tccttttggt aaacgatttg
     2461 aacgtacagc aacatctggt caattaaatg aaaaatacgc tgacaaatca aaagaagact
     2521 tgcacgacat tgaagaaact gctacaatcg ctggtcgttt aatgactaag cgcggtaagg
     2581 ggaaagttgg ctttgcgcat atccaagatc gcgaaggtca aattcaaatt tatgtccgta
     2641 aagattctgt tggcgaagaa aactacgaaa tctttaaaaa agctgattta ggtgacttcc
     2701 ttggtgttga agggcaagta atgcgtacag atatgggaga actttctatc aaggctactc
     2761 atattactca cctttcaaaa gcactccgtc cacttcctga aaaattccac ggcctaactg
     2821 atattgaaac aatctatcgt aaacgtcatc ttgatttaat ctcaaatcgt gacagctttg
     2881 accgttttgt aacacgctca aaaatcattt cagaaattcg tcgctttatg gatagcaatg
     2941 gtttcttaga ggttgaaaca cctgttcttc ataatgaagc tggtggtgct tctgctcgcc
     3001 catttatcac acatcacaat gcacaagaca ttgacatggt tcttcgtatc gcaactgaat
     3061 tgcatctaaa acgtcttatt gtaggtggta tggagcgtgt ttatgaaatc ggacgtattt
     3121 tccgtaatga agggatggat gcaacccaca atccagagtt cacatctatt gaagcctacc
     3181 aagcctatgc tgactaccag gatattatgg acttgacaga aggtattatc cagcatgtta
     3241 caaaaactgt aaaaggtgat ggaccaatta attatcaagg cacagaaatt aaaattaatg
     3301 aaccattcaa acgtgttcat atggttgatg cggttaaaga aatcactggt attgacttct
     3361 ggaaagaaat gactcttgaa gaagcacaag ctttggcaca agaaaaaaat gttccgcttg
     3421 aaaaacattt cacaacagtt ggtcacatca ttaatgcctt cttcgaagaa tttgttgaag
     3481 acactttaat tcaaccaact tttgtctttg gtcatccagt tgaagtgtct ccacttgcta
     3541 agaaaaatga tacagaccct cgttttacag accgtttcga actctttatc atgactaaag
     3601 aatacgctaa tgcttttaca gaattaaatg acccaattga tcagctatct cgctttgaag
     3661 cacaagcatc tgctaaagaa cttggcgatg atgaagcaac tggagttgat tacgattatg
     3721 ttgaagcact tgaatatggt atgccaccta ctggtggact aggtattggt atcgaccgtc
     3781 tctgtatgct gttaactgat acaacaacta tccgagacgt cctacttttc cctacaatga
     3841 aataagaata tattaaaacc catccttatt taaggtgggt tttaatatta cctctactga
     3901 actttgttag gtttctgatt tatcgctcac gcaatttttt atcaaggttc accatttcaa
     3961 tagccgaaag agcacaatca taacctttat ttcctgcttt tgttcctgcc ctctcaatag
     4021 cttgttcgat gttatccgtt gttagaacac caaagatatg agggatatct gactggctat
     4081 ttaaatgacc aatacctttt gtcacctcat tacaaacata atcataatga tcagtgctgc
     4141 ctttaatgac taccccaaga cagataatag catcataatc tttatataat gccatttttc
     4201 tagccatata ggggatttca aaagcaccag gtacccatac aatatctata tcttcttctg
     4261 aaacaccatg tctcagcagt ccatctactg ctcctgataa taatttagat gtaatcaatt
     4321 cattgaaacg tgagactacg attcctattt tcatttcgtt tgccactaat tgtccttcaa
     4381 taattgtcat tttttcttcc tcctttaatc taaaagatgt cccatttttt cttttttagt
     4441 ttttaaataa ttaaggtcat aagctgttgc tgccacctgt aatggcactc tgtttttgac
     4501 gcaaattcct tctgcttcaa gttgttggat tttatctgga ttattggtta gtaagtttat
     4561 agaatttata cctaaatctt ttaaaagttg agcactaact ccatagtctc tctcatctcc
     4621 ttcaaaacct aaagccaaat tagcttctaa agtatctaag ccttcttctt gcaaatgata
     4681 agcttttaac ttattaatca aaccaattcc tcgaccttcc tgtctcatat aaagaaggac
     4741 tccacgacct tcagcctcta tttgttggag ggcattagcc agttgttccc cacaatcaca
     4801 tctcaaggaa tgaaagacat cacctgtcaa acattctgag tgtaaacgac ataaaacatc
     4861 ttcaccacta ctaatatccc ctttaacaag ggcaacatgt tccttatttg ccacaacttc
     4921 gctatatcca taagccgtaa aatgaccgaa ttgcgttggt agctgaatct caatttctct
     4981 aaccacccct ccctcttctt gacgtctata gtcttgcaat tgttttatgg tagcgatagc
     5041 taaatcgtgc ttttgagcaa atgctaataa ctcatccttt ctcatcatac taccatcttc
     5101 agccattatt tcacaacaaa gtccacattc ctttaatcca gctaaacgac atagatcaac
     5161 ggttgcttca gtatgtccat ttctagctaa aacacctcct tccttggcta acaagggaaa
     5221 taagtgccca ggacgtctaa agtctttagc tttgctactg tcgttagcaa ccatcttagc
     5281 tgtcaaagct ctatcttccg cagaaatccc agtactagtt gatagatgat caatagatat
     5341 ggtaaaagct gtttcatgat tatcagtatt atggctagcc atttgagcta attctaactt
     5401 ttcagcatag taattagaca gaggtaagca aattaatcct ttcccaaatt tagccataaa
     5461 attaatcgac tcttttgtca ccatttcagc cgcacaaata aggtctcctt cattctctct
     5521 attttcgtca tccatcaaca ctaccatttt ccctgatttg atgtcttgta aaagtttttt
     5581 tattggacta aaggccatta gctttctcct ctctcttagt agccattttc tgataaaaaa
     5641 gaccaatcca ttaccgatga agtgtgatta gttctattag tgggtgctaa aaatttctca
     5701 gcatatttgc ctaaaacatc cacttcgata tttaataaat ccccaacctt ttttaaggat
     5761 agattagttt gagccatcgt gtggggaatg accgaaactt caaaagaatg ctctgtaaat
     5821 gataccactg ttaagctaat cccatctaga gccaccgaac ctttctctat aatataagac
     5881 ataatactag gtggagcatc aattgaaaat ctgacagcaa tatcatcttt ttggattttt
     5941 tctatttttc caaccccatc cacatgtcca agaacaaaat gcccccctaa ccttgagtca
     6001 cttctgagtg ctaattctaa gttaacttta gaatgaagtc gtaaagcagc taagcttgtt
     6061 cgcatcatac tttctggcat gacgtctgct ataaaagctg tttcactttt gtcaagaacc
     6121 gtcaaacaaa caccattaac agctacgcta tccccttttc ttaataaagg gactagtttg
     6181 ggagcatcaa tacttaattg ttgggacttt atgccattcc gaatacgact gacttgaccc
     6241 atttcctcaa taattcctgt aaacataatg ttctaccttt ccttcaataa caacgtcaga
     6301 atccatacga ctaagttcaa tatcttttac tctgaaagct tggttaagcc aatccaaacc
     6361 ttctccagaa atagctgttt ttgctttcag acctcctatt attttgggtg caataaaaac
     6421 aattagtctg ttaacaatac cagcttttaa agctgaaaaa tgtaaactac ttcctccttc
     6481 aataagaagg ctatcaatcc cttcttgtcc taaaatagtc attaatactt tcaaatccag
     6541 ctttccatct tttcttggga ccttaatcaa acggcaaccc atttctttta atgtttgttg
     6601 ctgagcaaga ttatctgaac atgttgcaat ccatgttggt attgttttag cgctcttaac
     6661 taggtaagag tctaaaggta tcttcaactg actatcacat acgatcctca caagcgcttc
     6721 acctttagga atacggcaag ttaaacgtgg attatcagct aatacagtat tgataccgac
     6781 cataatagca ctacattttt gcctcaattt ctgaacagat tgtcttgagt gctcatttga
     6841 tatccactta gaatcgcccg tctttgtagc tatcttccca tctaaagtca tagcatattt
     6901 caaataaaca aaaggctgct tataggtcat gtgaaaaata aatctttcat tcagagcatc
     6961 gcattcttct cttaagatac caacttcaac atttaaacct tcttttctca acaaggcaat
     7021 cccttttcct gaaactaaag gatttggatc taaacttcca actactactt ttttgatacc
     7081 agatttaata agagcttcag tacaaggtgg ttgcttacca acatgacagc atggttctaa
     7141 agtcacgtac agagtacttc cagagatatc ctcatcagca tttttaatag cctgtctttc
     7201 cgcatgtaaa tctccaaatc gtttatgata acctttggaa attatacgat catctttaac
     7261 aataacagca cctactaatg ggttgggagc aacgaacccc atacctttct cagcctcttt
     7321 caaggctaat gccatgtagt cttctttttt catcatttcc tttttccttc taaaataaaa
     7381 acccttagct tctaaaatag aaaactaagg ggagggatga gaagtcgttg attatactaa
     7441 ttagcattat attttaggta gcacaaaaaa actaaagact ccctatagaa aactataggg
     7501 agtctctcat taatccagca caaataaact gcaagtagca gacaatcact gaattcttct
     7561 ctcatccaga ctttactgtc ggtactggaa tcacaccagt tcatgctcga aagctcgcgg
     7621 actataccgc cggttaggat tttcacctga ccccgaagaa attctatttg acagttattt
     7681 tactaaactc atttttattt gtcaataaaa ataatgtgtt ttttattata aaataataat
     7741 taagtagctc atctattctt atctctacaa gacagtatat ctataccata aagcaatctt
     7801 tcgaactaat caaacaagaa tcgctagtat tattggatta tatttcttaa aaaattcact
     7861 acagatatag tttactccac ttaaataact taggtaaatc tacggaacta aaaataatcg
     7921 atagaaatct gttattttgt tatttcaaat gtaactgtta agaaaataat tctctaaata
     7981 ctaaacagca cctcaattga ttattaagta gcgttaattt ttaattgttt ttctattttc
     8041 tatttgttat aatggcttga gattactaga aagtatagta ttacatgaat caaaatcatt
     8101 ccgtcaaaag atccttatcc gaagttaatc agagcgttga ggttcctcac aatagctctt
     8161 tttggaacac tttaagagct tttcttggac ctggagcttt ggttgcagtt ggttatatgg
     8221 atccaggtaa ctggattact agcgttattg gtggagctac ctatcgttac ttactattgt
     8281 ttgttgtttt agtatcttct ttgatggcga tgcaattaca acaaatggct ggcaagcttg
     8341 gcattgttac acgacaggac ttagcacaag caacagcttc tcgactccca aaaccgcttc
     8401 gttacctgct atttattatc attgagttag cccttatcgc tactgattta gcagaagtca
     8461 ttggctctgc cattgctctt cacttattat ttggctggcc cctgttactg tctatcatga
     8521 ttactatttt ggatgtattc ttacttttac tcttaatgaa gttaggcgtc caaaaaattg
     8581 aagcctttgt ttctgttctc atattgacaa ttttgataat ctttacatat ttagttgttt
     8641 tatcacaacc tgatttagat gctatgttca aaggattttt accacaccat gagcttttta
     8701 acatatcaca tgaagggaaa aatagtcctt taaccctcgc tcttggcatt atcggcgcaa
     8761 cagtcatgcc acataatctt tatttacatt cttctttatc tcagactaga cgcgtggatt
     8821 atcataataa atcttcaata aaaaaagctg tacgtttcat gaccttagat tctaacattc
     8881 agctatcact tgcatttgtc gtgaattcgc tactattagt cttaggagct agtctatttt
     8941 atgggcatgc taatgatatc agtgcctttt ctcaaatgta tcttgcccta tctgataaaa
     9001 ctattactgg tgcagtagcc agttctttcc tttctaccct ttttgcagtt gccctactag
     9061 caagtggtca aaattcaacg attacaggta ctttaacagg acaaatcgtt atggaaggtt
     9121 tccttcactt caaattacct caatggttaa taaggctttg tactagatta ttaactcttc
     9181 ttccaatttt tgttatcgcc ctactagttg gaggcgaaga aaatacgtta gatcaactaa
     9241 tcgtctattc acaagttttt ctttccttag ctttaccatt ttcaatcttt ccattgattt
     9301 atttcacgtc gcaaaaatca attatgggag agcatgctaa tgctaaatgg aatacctatt
     9361 tagcatacct tgtcgccatc attttaacat tactcaattt gaaattaatc atggatttac
     9421 tttaatacaa tacaaaaaag ccccactaat gaactgaacc tctatcatga gacatacatc
     9481 taatgataga ggaaagtaca taatgatgga gctttttgta tttctttcaa agaacccgtg
     9541 agtaagggct agtatttttt tgcttgtacc tctccttgac attataataa ccgatgatga
     9601 aaaacgatac gccaaggata attgctgcaa tcaaagataa aatttggata ctaaaatata
     9661 ggtgttgaaa atttccttct aaatgtgctt taaaaaccat caaccaagcc caaaaaacgg
     9721 ataagattaa gtaagcattt tgtaatactc tagctaggta aagacttaaa acagttataa
     9781 aaatcaaggc aatgatagct atatatacct ttggtaatgg gtaattaaaa tcaattttaa
     9841 ctaagtagac cgctaaattg accaatgacg caactagcaa ccatcctgca tgaattccaa
     9901 atgttattct aagcagtaat tttgatttat ttttacttag tatcttgata atgataacca
     9961 gaagaataca gtaagcaaaa attattattg ttgataggcc aatcaaatta ctcgtaaaaa
    10021 atacagtcca tatattatta actaatagaa gacccaaaaa gtatggaaaa atgctagcaa
    10081 agttatcttg gtaagattga ctcttactga atatagctct taatattact aaaaaggtca
    10141 ataaatagat gactgaccaa attgaaaagg ctaatggtgc tggtgtcaag agcgtttgat
    10201 aacgatcaga cagactcttc tgactgttcc ctgttaaaaa acctgttgcg gataaataat
    10261 tcactattat tgttaagata aagactaaca ataacacctt acttttaaat gaatgtctaa
    10321 tcatagaggc ctccttttac ctccattata attcttttag tttaaaaatt ctattttttt
    10381 aatcaatatg tttactgaat actcgtttgt ctcgacaaaa aaagtggttt ttgaaaccac
    10441 tttttattta tttacgaaaa gcatctaaaa tgactttaaa ttcttcatta gataaagtga
    10501 tacctttacc catttttgta tgatctggac tccatgtacg caaatcaaat ttagctggcg
    10561 ctccgttgaa gctcacacga ttcaactctt ttgtccaacc tttttcattt tcagatagga
    10621 ccaataactt ctcaacaatt tcaaatgtaa attctgacat tttatacctc cacttatact
    10681 attcgttatt tttatagctt tttgttctat ttaaatgaac ttcttctatt ttaacaattt
    10741 ttagtaatat tgaccatgta aaatgacaaa atagttataa aagtatctaa gctcttacag
    10801 tcttgctagt accatcattt tgataaaggt tcactaatcc ttctttacaa gcacgaatca
    10861 tatccccttt ttttactgga tttggtaatg ttatggttaa taattccatc ggatttggag
    10921 cacggtctat tttatttcct tcagcatcac gcagaccatc aataaaacat tcaaagtgac
    10981 ggaagccagg accatagaat tctacgcgat ctccttccat aataacatta cgctgacgaa
    11041 ttgtagcctc cattttagca ttgtcaaatg aaaccacttc cccaacaaat ttgtattgag
    11101 gaattttacg acgagctcca aagagttgtt cattttcagt tggtgtatgg tagtagaaac
    11161 ctgttgctaa ttcgcgttgt gcaaccttcc aaagttcatc aatcaagtct tctttaatag
    11221 cttcaaaagc ttctggactt tccatatagg catctacagc agctttatag caattagtta
    11281 ctgtagaaac ataatgaatg gatttcatac gtccttctat ttttaaacta tctacaccat
    11341 tttcaatcat atctggaata tgctcaatca tacacatatc cacagctgac attgagaaag
    11401 gttctggaat ctcgcctttt aacgattgac gttcttgtcc aaatggcata tcgtagaggt
    11461 catatttcca acgacatgac tgagagcaac cgccacgatt agcatcacga tggctcatat
    11521 ggtttgatag aacacatcgt cctgagtatg aaatacacat cgcgccatga acaaatgctt
    11581 ctatctcaac atctgtcctc ttcctgattt cagccaactc tgccatagta acctcacgtg
    11641 ctaatacgac acgagtaagc cccatctctt tccaaaattc aaaagtttca tagttcgtgg
    11701 aagaggcttg agttgacaaa tgaatttcta agcctggtgc ttctgtagca caaataacaa
    11761 taagagcagg atctgaaaca atgactgcat ctagtcccat atcacgtaat tcacgaaacc
    11821 acggacctgc cccaagctca ttaccttcat gagtaaccat attagccgct acataaactt
    11881 tagcatctct tgcatgagca tagtttattc cctcttgtaa ctcttccata gaaaagttac
    11941 cagctctact tcgaagacca tacgcttgac ctccaacaaa tacagcatct gctccataat
    12001 caatagcaac ttttaatttt tctagtgttc ctgcaggtga taaaacctca gggcgttttt
    12061 ttacattaga catcttatat ttccttctat tttcaagata aaagaattat tttctatctt
    12121 aattaacata ccaatcgata tgcagttctt agtgtaattg gctacgcttt gaaatagaaa
    12181 actttcacgt agcaaataaa tcttatttta ctgttgatgg gtcaaaatca taaaatccag
    12241 tatctaaacc acgtccttta gggtgcagtt ttctaattcg ttcatcaaac aagaaagcct
    12301 gatcctgagt gaatgttcca ttttcaatca aacgagctgt ttcgacaaaa atttcagcaa
    12361 tagcaacaaa attatctcct ggacaataaa taccatctaa tttccagtga gtaaagtgat
    12421 gttcaactag ttcagtcact ttagtcatca tattgatatc attgttaata aaaatatgag
    12481 tcccatgctt atcttcgtaa atagaatagt gactttctgg atcacctggt tctgctaaaa
    12541 aaagaccacg ctcacgtgtt ttttcatcag tgatatgagt aaaattataa tagttttgca
    12601 ataacggacg cttagagtga tgaattacag aagccccata aactaaaatc tcagcaggaa
    12661 attcaagatt ctcagacatc ttaaaaagtt cttcagaagg tatttcacgt gctaaaaccg
    12721 tttctactgc tccatgttgt ccccaaaaat taacttgacg acttgatgta acgaagacag
    12781 aagtgtcgta gataagtttg aagttataac catcacgctt gttgatataa aatactcctg
    12841 catcaccaac taccaaataa tccacattaa tttctttcat caactccaaa aatggtttga
    12901 tattatccat catctcttga tgcatgagtg cattacaagc tactgtcaat tctttaccag
    12961 catcatgtac taatttagcg atttcacgca attcatcgtc tgaaaatgaa tgtggtaaac
    13021 gaagaccgta attttcttcc cctacataga tgcggtcaat tcctattgct agcaattgct
    13081 ttacttgttc aatagattca gctgtcgctg tcaaaattat tttttccata attagtatta
    13141 taacaaactt tcagcatttt taagcatatt tcttgacaag aataaataaa aatattcttt
    13201 tcgaaaatta tatcacttta gaggttaagc actatcagat aagaaaaaat atagtattgt
    13261 aatattatat aaaattatat ctattttttc ttttccaatt taaaaaagta aggaaagtag
    13321 taaaaaatac ttttagtatt ctactactaa tatgggcaat gtttttgttt ttttgagaaa
    13381 aaacctttat tttaactcat agtatcaaaa aactagtgac aaactctatt atttgtgata
    13441 tgatatgcta gtatcaaagg agaaagctat gtcatcacca cttaagcaag agcaaactca
    13501 cgaaagcaaa acagagcaag ctcaaccaaa atttcaagta tttcaagaat ttgatcacag
    13561 tgctaagttg cgtgagctag tcttttttgc tcgtatcgct ctcttcagta taaccactgt
    13621 gctaatctct ttcttattac tagcactaag tttaacacct gtctggagtt tcttactagc
    13681 gatgctggca agtctagcag ttacatctgc agtctcttca ttcatttggt cactcagaca
    13741 ataaaaatct cataaaaaag aagctactta tagtagcttc ttttttattt ttctttatct
    13801 tctgtgtttt ctttataatc aatgacaata tcttctactt ttgtttcagg agccttatct
    13861 tcttttttag cagtatcctc gtctgagaat tttgatttag cttgattgac aaaatcatta
    13921 gcaaagtcaa ctacttctcc gcttttttcc ttaacggctg agacgatatc ctctgttgtc
    13981 aattcacctg attcaaattt acctttataa tctttaaaag tatcaacagc taaatttgaa
    14041 tattcacttg ctttatcttt agctatttga tgatattctt ctggattttc tttatattct
    14101 ccatagaatt tttctgcatt tttccttagc tcttttcctt tttttgttgt taaaaaataa
    14161 gctgctgctg cacctgatgc agcactgata actaaagttt taaataattt actcattttt
    14221 tgtctccttt tttacgaaaa atttttgaag ccgcttttcc aatagctaaa gctgaacctg
    14281 ccttagtaac acttgaggtt gcactacttg ccttttgtcc aatatgtctt gcctgtaggt
    14341 ttaaatcaga tacagattct gataagtcag caatagctac aaaaagagga tcaatagtag
    14401 atactttacc attaacatca tcaactaaaa cattagcttt ggctaaaatt tcattagttt
    14461 ggtaaagagt cacgttaaca tcgcttgtta atactttaat agtttgcttg gtttcctcaa
    14521 tcgtttctga tacttttttc agaacgaaaa gtattcctaa aactaaaaca acaaacgcaa
    14581 tggcaataat taatacagca atttctatca taatacctcc tctgtttaat aatatgaaat
    14641 atgttgttca cgacgacgat aaataatcat gacaattccg ataataacta aaataactga
    14701 taaccattgt gacacgcgca aacccgccaa ataaaggcta tctgtcctca tcccttcaat
    14761 aatgaaccgt ccacatccat accaaactaa ataaaaacat gccacttcac cttgttttaa
    14821 agtgcgcggt cgatgacgaa tagacatgat gatgacaaaa cctaggaagt tccaaagaga
    14881 ctcatataaa aatgtaggta ctcggtaagc tccatcaata tacatttgat tttttataaa
    14941 gtttggtacg taattcagat tttttactgc cctaccgtat gcttcttgat taataaaatt
    15001 cccccaccta cctatggctt gggcaatcat cactcctgga gcagcaatat ccagaaaatc
    15061 aatgggatta atcaagcgac gatatgaaaa aatcacaagg agaatggccc cggtaattaa
    15121 ccctccgtaa atagcaatac cgccattcca gatggcgata atctctactg ggtgcttaga
    15181 gtaataagcc cattcgaaga taacataata aatacgagct ccaacaatgg atagtggaaa
    15241 agccatgaga atgaaatcta gaatatcatc cgacttaatg ttcttacgag gggcctcttt
    15301 cattgctaag taaactgcta acaacatacc agacactatg caaatagcat accagcgaat
    15361 agaaaagggg cctaatcgta ttgctactgg attaatcatc attgacctcg ttctgactaa
    15421 tcaaatgtgt taaacgatcc tcaaaagtct gggtagcatc aaaccccatt tgtttagctc
    15481 tgtgattcat agccgctgct tcaataacta ctgacacgtt tcggcctgtt ttaacaggaa
    15541 tacgtatccg aggaacctta actccagata attcgatctc ttcattgcca ttcccgagtc
    15601 tatcaaaaac ttttccagtt tcaaagtttt caaggtatat agctaattgc acttgcgatg
    15661 aatctttaac cgcactagct ccatataggc tcataatatc aatgattccc acacctctaa
    15721 tttcaagtaa atgacgtaat atctcagcag gctctcccca aagcgtctct tcgtctttag
    15781 cataaacatc aacacgatca tctgctacca aacgatgtcc tcgcttaact agctctagtc
    15841 ctgtctcact cttaccaata ccggaatcac cttgaatcag aacacccata ccatagatat
    15901 ccatcaagac accatgaaca cttgtcctct cagctaagca agaatctaaa taccaagaaa
    15961 cttcacctga taaacgactt gtaggggcct tactttgaag aatagcaata ccggtatctt
    16021 tagccgcctc atacatctct tctggaattt ctaaatcacg tgctacaaca atagctggtg
    16081 tttctttttg gaacatttca cgtaacactt gataacgatt atgtcctgtc atagccatta
    16141 aatatgacca ctctttcata ccaacaagtt gcaaacgctc tggtgcataa taatcaaaat
    16201 accctgtcat ttctagacca ggacgtgaaa tgtcagctgt tgtaatccgc ttagataata
    16261 gatgttcatc accatagatg acatttaact ttaagcggtc aactaacatt tgaacagtaa
    16321 ctgccatact tactccttct tataaaagtg aatatcccta ttgctatacc ctacaattta
    16381 aagttgagca aaggtactaa acactttcat taatttacac ccacattata tcatactttt
    16441 tactaaaagt aagcgctaac gcgtttttct gaaaaaaaaa agatattttg aaaaagtggt
    16501 ctaaataaag ctaagaaaaa aggagattca tctagcaaat ctcctttata tgaattacca
    16561 gaaccaaccg tcatcatcaa caggttcagc atctttacgt cttctgggac cttgtcttct
    16621 tgtttcaata atatcttctt tatagggtaa gaaaacagct aataaaatat atagcaaaag
    16681 accaaatttg gtaaaataca aaatcagtgc aatcaacacc cgtgaaagag ctaaatccca
    16741 gttatactta tcagctagac cggcgacaac accgcagacc aacttacctt ttcgttgttt
    16801 gtagaagctt gatttcatac ctatccttct ttctatctac tctatatttt aattataact
    16861 actttgacag taaaattaaa taggccttac gactgatttt aaaaactctc tatcaagcga
    16921 ccatgacaat aaccacaagc aaacttgcgt aaattaacac gtctcctacg tcggtatact
    16981 tgaccgcaag attggcaaga gtaatgatga taattcactt taacagccgt aactctaggg
    17041 gtatatcgta agccatcgac tttttctagt aactctttaa aagctttatc cctatgttga
    17101 tatcctttac cttgtatata gagatgataa tggcaaagct catgtcttac aatcttacga
    17161 aaaatatcaa taccaaactc acgataaatt ttgggattaa agtcaaggtg tctatcgttt
    17221 ggaaagaagc gtcctccagt tgttcttaag cgattattcc aatgtgcagt atgtaaaaac
    17281 tgacatccaa aatcttctat agacaccttc ttaacataat tagtcaagtc cgtctggctt
    17341 gataagacta agtccaattc tgctacgctc cgcatctacc tctacaaccc aaacagttac
    17401 aatatcacca acagagagat atttgcttgg atgttgtaaa ggaggcattt ttctagtttt
    17461 tttatcgcgt tttcgcttaa taaggcgaga ctggtggatt agaccatctt catgaacacc
    17521 aatatcaaca aaagcaccaa aatcaacgac gtttcgaaca gttccttgca attcctgtcc
    17581 aacttttaaa tcactgacat ctaaaacatc atgtctcaaa acaggcgctt caaaatcatc
    17641 acgcaaatca cgacctggtt tcaaaagatc ttctataatg tcttttaatg tttcttgacc
    17701 taccccaata ctttctgctg ttgcaattaa gtcaaggttt tgtaacttct ctttagcaag
    17761 atcatctaat tctttgatag tcagttggtc aagcaacttt ttaacagctt cataagactc
    17821 agggtgaaca cctgtattat ccaagaaatt tttagcatta ggaatacgta agaatccagc
    17881 agcttgctca aaagcttttg ctcctaatct gggcactttc ttaatttcag cacgtgactt
    17941 aatttgccca ttctcttcac gatatttaac aatattttca gatattgttt tatttaaacc
    18001 tgaaacatga gctaacaagg ctggacttgc tgtattaaca ttaacaccta cttggttaac
    18061 tacagtttca acaacaaaat caagattttc tgctaatttt ttttggctaa catcatgttg
    18121 atattgaccc acaccaatag attttggatc aattttgact agttctgcta atgggtcttg
    18181 cagacgtctt gcaattgaga tggccgaacg tttttcaacc gttaagtctg gaaattcatg
    18241 acgtgccaat tctgaggctg aataaacaga ggcaccactt tcgttaacaa tcacatagga
    18301 tacgtctgga aaatcttgta aaacttctgc aacaaatgct tctgattcac gactcgctgt
    18361 tccattccct atagcaataa tttctatatt gaattctttg ataagttttg ctaactctat
    18421 ttttgattgt tcgatttttg cttgattagc tggaggaact ggataaatca cttgtgttgt
    18481 cattaatttt ccagtttggt ctactactgc taattttgct cctgtacgaa aggcagggtc
    18541 aaaacctaac accattttcc ccttaagcgg tgagactaac aataagttac gaagattctc
    18601 tgaaaacagt gaaattgcac catcttctgc tccttcactt agttctgtac ggatacggcg
    18661 ttccatagct ggaacgattt ttttcttgac agtctgtaca ataaggtcat ctatatattg
    18721 gctagtttct ttaaatcgaa cagcaaaaaa gcgaaacatt ttttcaaggt tatgctcaaa
    18781 attaacctta agaacaccca ttttctcacc acgattcaat gctaaaactt gatagccatg
    18841 gagtttactt attttttcag aaaagtcata gtagattttg aagacctgtt tttcatcaag
    18901 tgactcatct ttaacaactg cagtgattga gctgtagttc cagatctcat tatatgtcca
    18961 tgaacgtaat ttattatcct cagaaaaagc ttcgataaga atatctactg ctccagaaag
    19021 tgccttcgta gttgtctcaa aaccatctgt aagatagttt tgagcttctt cctcaaggtt
    19081 atccttattt tgtaaaataa gacgtgctaa aggaaaaaga cctgcttcac gtgcaatcgt
    19141 agctttcgta cgacgttttt ctttataagg taggtagagt tcctcaacat ctgctagttt
    19201 agttgcttct tcaatagcct ttttgagttc ttgagtcaac tttccttgtt cttcaatttt
    19261 ggctaagacc gtagttttac gatctgaaag agctgtcata ctcttatcaa ggtcaataat
    19321 tgatttgatt tgaacttcat ccaaattacc tgtcatttct ttacgataac gggcaataaa
    19381 aggaatagta tttccttcat ctgttaattc taaaaccttt tcgatttgtg acgctttaat
    19441 acctaaatca atagcaattt gtgtaatatt taaattttcc ataacgcttt tattatagca
    19501 tatttttgac ttacgaagag ttttaaaaag cgatttcaag atagcgtttt tataatctaa
    19561 caataaaaaa acagaccaga tctgctttaa ttcttttcaa aatattttcg aacaattggt
    19621 gctacttcct taccataaag tttgatagca tttaggacgt ctttgtgagg catagagcca
    19681 actggtagat gtaacataaa acggtctaac tcaagtgctt caacaagtcc tataatttta
    19741 tgtgcaacca cttcaggact tcctacaaaa atagcacctt ctggacctac tgaacgtaaa
    19801 tactgctctt tggtcatttc agaccaatga gggcgtccct tagcaatatt atcgacggtc
    19861 tgtttcgtag ggaaaaaata acggtcaata gcggtttgat tatcctcttc aatccatccc
    19921 caagagtgag cagcaacttt tagttgttct ggtttgtgtc cattacgact accaacttct
    19981 ttataaatat ggaccaattg acgaaatgct ttgggattcc caccaatagt tgcataaaca
    20041 attggcaaac cttgttctgc aatacgaatt gtagaatcaa catttcctcc tgttgccacc
    20101 catattggta actgtctctg taatgctctt ggataaattg gtcgctcatt aactgtttgt
    20161 gtcaaatgac ctttccaatc gagatttgtc gctgagttaa ttgctaacaa catatccatt
    20221 ttttcattaa ataaatcatc ataatccgct aaatcgtatc caaacaatgg aaaagactca
    20281 ataaaggaac cacgccctgc cataatttct gctctaccat ttgaaagtgc gtcaatcgtt
    20341 gaaaattgct ggtagacgcg aataggatca ttggaagaga gaatcgttac tgcactagat
    20401 aaacggatat tattagttct aaccgctcct gctgctagga caatttcggg tgcagagacc
    20461 gcaaaatctt cacgatggtg ctctccaata ccatatacat ctaaaccaac ttgatcagct
    20521 agttcaatct cagcaactaa ttggcgaatc ctctcaggat gtgaatagct ttggtttgtt
    20581 tcttcaagga ttgttgtctc tccgaatgtt gtaataccta attcaatcat aatcttacct
    20641 catcaatcta ctaataacat tttatcacta tatagtatca tttccaatcg atttgttttt
    20701 ttatgttaaa aataagctaa tatagtataa taggttatta tataaagaaa gaggtgccat
    20761 taatggctat tacgcacaaa cgtcaagat
//
LOCUS       DATRSD010000038        20438 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRSD010000038 DATRSD010000000
VERSION     DATRSD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20438
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.038"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            274..1203
                     /locus_tag="VBD85_001090"
     CDS             274..1203
                     /locus_tag="VBD85_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7133651.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1260..2792)
                     /locus_tag="VBD85_001091"
     CDS             complement(1260..2792)
                     /locus_tag="VBD85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO7133652.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLISGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2901..3974)
                     /locus_tag="VBD85_001092"
     CDS             complement(2901..3974)
                     /locus_tag="VBD85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7133653.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3967..4743)
                     /locus_tag="VBD85_001093"
     CDS             complement(3967..4743)
                     /locus_tag="VBD85_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133654.1"
                     /translation="MKKFANIYLALVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4740..5534)
                     /locus_tag="VBD85_001094"
     CDS             complement(4740..5534)
                     /locus_tag="VBD85_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133655.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLVILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5518..6672)
                     /locus_tag="VBD85_001095"
     CDS             complement(5518..6672)
                     /locus_tag="VBD85_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133656.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6721..7623)
                     /gene="murB"
                     /locus_tag="VBD85_001096"
     CDS             complement(6721..7623)
                     /gene="murB"
                     /locus_tag="VBD85_001096"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO7133657.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7767..8255)
                     /gene="folK"
                     /locus_tag="VBD85_001097"
     CDS             complement(7767..8255)
                     /gene="folK"
                     /locus_tag="VBD85_001097"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO7133658.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYFKP
                     VE"
     gene            complement(8252..8614)
                     /gene="folB"
                     /locus_tag="VBD85_001098"
     CDS             complement(8252..8614)
                     /gene="folB"
                     /locus_tag="VBD85_001098"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO7133659.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRA"
     gene            complement(8616..9419)
                     /gene="folP"
                     /locus_tag="VBD85_001099"
     CDS             complement(8616..9419)
                     /gene="folP"
                     /locus_tag="VBD85_001099"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO7133660.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(9423..9986)
                     /gene="folE"
                     /locus_tag="VBD85_001100"
     CDS             complement(9423..9986)
                     /gene="folE"
                     /locus_tag="VBD85_001100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO7133661.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAFEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(10005..11267)
                     /locus_tag="VBD85_001101"
     CDS             complement(10005..11267)
                     /locus_tag="VBD85_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO7133662.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(11269..12156)
                     /gene="rarD"
                     /locus_tag="VBD85_001102"
     CDS             complement(11269..12156)
                     /gene="rarD"
                     /locus_tag="VBD85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7133663.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(12143..13009)
                     /locus_tag="VBD85_001103"
     CDS             complement(12143..13009)
                     /locus_tag="VBD85_001103"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO7133664.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(13011..14294)
                     /locus_tag="VBD85_001104"
     CDS             complement(13011..14294)
                     /locus_tag="VBD85_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO7133665.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVADLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            14471..15358
                     /locus_tag="VBD85_001105"
     CDS             14471..15358
                     /locus_tag="VBD85_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7133666.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(15381..16928)
                     /locus_tag="VBD85_001106"
                     /pseudo
     CDS             complement(15381..16928)
                     /locus_tag="VBD85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(17071..18447)
                     /locus_tag="VBD85_001107"
     CDS             complement(17071..18447)
                     /locus_tag="VBD85_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO7133667.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASHDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDAFVNEH
                     LVFTPNSD"
     gene            complement(18521..19528)
                     /locus_tag="VBD85_001108"
     CDS             complement(18521..19528)
                     /locus_tag="VBD85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO7133668.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(19636..20322)
                     /locus_tag="VBD85_001109"
     CDS             complement(19636..20322)
                     /locus_tag="VBD85_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133669.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQVADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6951 a         4088 c         3045 g         6354 t
ORIGIN      
        1 taattgagta tattaataaa tttggtcata ttaatacaac aaaaaatcct tacatgagat
       61 aaggagatta aacgaccctg taaggatttt gaagttatta gaaacactct aagcaaaaat
      121 tttaaagcgt tttctctatg acttatatta tatgtcatgt tcctatacaa aacttacact
      181 atcctttcaa aaaataatac tatcctacga aacccatttt ttgatagttt tctgttataa
      241 taatactatc ctacgatttt agaaagaaaa atgatgaact cacttgataa tttactatca
      301 cataatttag aagataaccg gcatttatta ccctatgaac acatgcatac tgaggttaga
      361 aatggttacc ctgacatttt atttcactgg catccggaac ttgaaatttc atatgtccac
      421 gaggggaccg cacgttatca catcgattat gattttttta atagccaatc tggtgatatt
      481 atcttgatcc gtccaaatgg tatgcattct attcatccga ttgaaaataa agagcatatc
      541 acagatagca ttaaatttca cttagattta ataggatatt ctattgtaga ccaagttagc
      601 cttcgttatt tacaaccgtt gcagactagc tcttttaagt ttattcaatg tatcaaacct
      661 agtatgacag gatataatga tatcaaaaat tgtttgtttg atatttttaa tatttcaaaa
      721 gaagaaaatc gacattttga gctactccta aaagctaaat taaacgaact cttgtattta
      781 ctctattatc atcaatatgt cattaaaaaa catactgatg atacctacag aaaaaacgag
      841 agaattcgtg atctgattga ttatattaat aataattacc agcaaaatct taccattgaa
      901 tttttagctg attatatggg gtatagtaag acacatttta tgaccgtatt taaacagcat
      961 actggaacat cctgtacaga atttatcata caagttcgtc ttaataaagc tagtgagcat
     1021 cttattaact ccactacggc cattattgat attgccaaca gcgttggttt taataatcta
     1081 tcaaatttta atcgccaatt taaacgctat tatcatacaa ctcctcgcca gtaccgcaaa
     1141 caatttacca acaaaaaaga cggtagtacg cttgtctata gaaatataaa tacaaaaaaa
     1201 tagaaactag ttaaatccat gactagtttc tattttttat tatttaagcg ttaattagtt
     1261 tacatcagct gctcacgtac ttctttaatt tccgattcat tcacaactaa aaaaatggtg
     1321 tcaccagcat tcaagatagt atttcctgat actacagccg atgtcttatg atgaatttga
     1381 gtcgttatta gcacattttc tggtaattct aaatctcgga catactttcc tgatatttta
     1441 tctgaaacag ttaattcaat caatgtaggt gtcattactt tatcttttgg attgaaggtc
     1501 attttagcca acatcgcttc ataaataggt tctcccttta ataaatccat gacaatataa
     1561 gataccattg ttacaattcc aatagccatt aattgtttta aatcgccaac catttctgta
     1621 accaaaatca tagctgttag tggtgctttt gagattgcac caaaatagcc cgccattcct
     1681 agaacaacaa ataaagatat ctgctgatga gatattatac caaagtactg catacctact
     1741 gcaaaaaaag cacctgctaa agatcctaaa gctagaattg gtaaaaaaat tcctcctggc
     1801 aagccactac tgtaactcaa catactccat aaaaaacgaa tgaggaaaaa taatcctaac
     1861 atcatcagac taagattact cctaggcagt gatacaatta agccatttcc accccccaat
     1921 aattgtggga aatagtaacc aataggaaga ataaaaatta ccgctaatat accatatagg
     1981 tgagatggta gatgaaatag tttacctaaa taatcataga tcacatgaaa tctcaaaatt
     2041 acccactcat agataaagcc taagataccc aaaaagagac ccatgagtaa aaaaatccaa
     2101 taaaagttga gatttaaaga tggtaattct gatggtaaag ctaaaactgg ggttaaacca
     2161 aagatattaa gtgagacaaa gtttgccact aaacttgcta ccaaagctgt tatccaaact
     2221 aagcgagaaa aatgatggta aatttcttca actacaaata aaaggcctgc aataggagca
     2281 ttgaaggctg ctgaaagacc tgctgctgca ccactagcaa ttaaaacacg tttttccatt
     2341 cgactagcat tgagatactg tgcaattccc ttacctgtca tagctcctaa ctgaatactg
     2401 ggaccttcgc gaccaagcat aagtcccgaa gaaatagcta aaatacccgc aatgaatttc
     2461 ttccatacaa tactaaacca gtctggcgat agcattcctt taagttcacc ttcaacatgc
     2521 gggatacctg aacctttaat atcaggatca gatttgataa aaaaacctat tatcactgca
     2581 gcgataatac tagtaaccgt aatgctcaat aaaataattg gttgataatg ggcactttta
     2641 taaagttcca taacaactaa aaaaattttt tcaatcgcta atcgaaatat gctgacaaca
     2701 aatcctgata ttaagcctac tactattcct ctccaaacaa atcccagaat tgattcaagt
     2761 gaaaactgaa attcattttt atgattttcc atattttcct ccttttctta caattatttt
     2821 aacaaaaata tcacaaactt tctaggaact cttggtaact aaaaatacct ctcatcagag
     2881 aagtactttt agttaataga ttacttacga tacattttaa attgtaagta caaatcatta
     2941 taaataccaa gccatttttg acccaagtta ttataaactt ctaaatgatc aattgtttta
     3001 tcaggaggat aaaatgcttt atcatttttg atatctgctg gtaacagcgc ttttgctttg
     3061 agattagggg tcgcataacc aatatattct gcattttgtg ccgcattttt aggttccatc
     3121 atgaaattaa taaaggcata agcttctttg cgatgcttta ctgttttggg gattacaata
     3181 ttatcaaacc aaaggttcga tccttcacta ggaacaacgt aatgcaaatg cttgttccca
     3241 tcaagcattt ctcgtgcttc tccagagaat gtaacaccaa tcgctgcatc tccctgaatc
     3301 atatagcctt tcatctcatc agcaacaata gccttaacgt ttggtgttag agcatctaac
     3361 tttttagaag cggcttttaa ttctgatatg ttctttgtat tcaatccata tcccaaggaa
     3421 tttaaaccaa ctccaataac ctcacgtgca ctatcaacta gcatgatttt atttcgaaac
     3481 tctggacgcc ataaatcatc ccaatgttta ggaggagttt taactaattg atcattataa
     3541 acaatcccaa cagttcccca aaaataagga atcgaatatt tatttttagg atcgaaactc
     3601 aaatttttaa acctagctcc aatagcatcc caattagcta tctttgaatg atcaagttta
     3661 accagaaggt tttcttttat cattttgtca atcatataat cactaggtac agcaatatca
     3721 tatgtcgtcc caccctgctt aattttggtg tgcatagcct cattagagtc aaacgtttcg
     3781 tattgtactt caattccagt ttcttttgta aattttttta ataaggctgg atcaatatag
     3841 tctccccagt tataaataac taacttatct gagttaggtg ttgaactact cttcttttct
     3901 aagtaagtgg ttaaaccaaa gaggatcagg ataacaagaa taataccgcc taaaaaggaa
     3961 taaactctac gcattctttt ctcccttctc tttcgaaata tagtaataac caattaccaa
     4021 taaaatacta aataaaaaga caatagttga caaggcatta atttccaatg atataccgcg
     4081 acgtgcgcgt gaatagattt ctactgaaag cgttgaaaag ccattacctg taacaaagaa
     4141 agtaaccgca aaatcatcta gcgagtaagt gaaagccata aagaaacctg aaataatacc
     4201 cgacgacaaa tagggtaaca taacttcttt aagcatttgc caagttgaag ctcctaaatc
     4261 ataggaagca ttaatcatat catcattcat ttcttttaaa cgaggtaaga ccattaaaac
     4321 aacaatagga atagagaatg caacatggct caataataca gaagtgaaac ctaactgtaa
     4381 accaataaca gtaaatacta agagaaaact tgcaccaatc ataacatctg gtgctactaa
     4441 aaggatatta ttaagcgata aaatagaggt ttgatgtcta cgtcttacct gataaatcca
     4501 aatagctcca aaggtaccaa taattgtagc taataaggcg ctaagaaaag ccaaaaagaa
     4561 tgtttgcact aaaattagca ttaagcggct atcctgaaac agttctccat agtgactaaa
     4621 ggtgaatcca gtaaaagaat tcatatcccc acctttattg aaagaataaa aaatcaaata
     4681 aataatagga atataaagga taataaaaac aagtgctaaa tagatatttg cgaatttttt
     4741 catgctttcc tctcctttgt taaccacata atagcaacca tcactaaaat taaaataacc
     4801 cctatggttg agcccatacc tttattttgt gtgattaaaa aatgttgttc tatggctgtc
     4861 cctaaagtaa taactcgatt accaccaata agacgcgtaa gcataaagag acttaaacta
     4921 ggaataaaga cagattgtac acctgctctt acaccattca gagatagagg aaatataacc
     4981 ttctgaaaag tttgccatgc atttgcaccc aagtcatcac ttgcataaat gagattttga
     5041 tcaatatcat ccaaggcatt aaaaatgggt aataacataa acggtaattc aatgtaagct
     5101 gctacaaaaa gaaatgaaaa atctgtaaat agaatctgtt ttgggccgat acctataaag
     5161 gttaagaagg cgttaattcc cccttgttga ccaaatatcc ccataaaagc ataggctttt
     5221 aacaaaagat taatccatgt tggcaaaata actagcatta gccataattg cttgtgtttt
     5281 aattttgtaa gcaaataagc agctggatag ctaatcaaaa gcgttatgaa actaacgatg
     5341 cctgcatata ggaccgagtt aaagctcatt ttaagatagg ttcctgagct aaaaaacgtc
     5401 tggtaattag ctaaagtaaa gtgcccatta atatcaaaga acgagttcca agcaatcaaa
     5461 gcaacaggtg ctaatacaaa taagaataac caagccatat aaggaattga aaatagacta
     5521 gaggttttct tcatttctct cctcctcgat agcattgatt aaaccatctt catgatcgtc
     5581 agtgtctaca tattcttcaa tgcgcgcatc aaattcttct tctgtttcat taagacgcat
     5641 aatatgtata tcttctggag tgaaatctaa cccaataact tcaccctcaa tagctttacg
     5701 agttgaatga atcatccatt cattacctaa atcatcataa gcaataattt catagtggac
     5761 acctcgaaaa agttgtgtgt ccactttaac ttgaagtttt ccttcatcgg gtagggtaat
     5821 ttgaagatct tctggacgaa taactacttg gactgattca ttagggcgca taccgccatc
     5881 aacagcctca aagcgcttac cgttaaattc aaccaaataa tcctcaatca tcttgccaga
     5941 taagatattt gattcaccta taaaagttgc tacaaaatga ttgataggtt catcgtagat
     6001 atctactggt gttcctgatt gaacaatttc accttcattc atgacgaaaa tccaatcact
     6061 cattgccaac gcttcttctt gatcatgtgt gacaaaaaca aaggtaattc ctaaacgttg
     6121 ttgcagttcg cgcaattcat attgcatctc tgtacgcaat ttcaaatcca atgctgataa
     6181 gggttcatct agtaagacta ctttaggttg attgatgata gcacgcgcta tcgcaacacg
     6241 ctggcgttgt cctcctgata atttttgaat agctcgcttt tcaaaccctt ctaaacgaac
     6301 catttttaga gtttcttgaa cacgtttttg aatttctttc ttatccattt ttttcaactt
     6361 taatggaaaa gcaacgtttt caaaaaccgt catatgtgga aacaaagcat agttttggaa
     6421 aacggtatga acatcccgtt tattagtggg aacatcattg atgcgtttcc catctaagta
     6481 aatatcgcct gtactcgcct ctagcaaacc tgcaataata tttaagatag ttgatttacc
     6541 acttcctgag gcccctaata aggtgtaaaa tttaccttct tcaagttcaa aattaatatc
     6601 ttttaagaca accgtgttgc tgtcttcgaa tacctttgat acatttttaa atgcaatgat
     6661 tgggttagtc aattctcata aatcctccat ttttctgact aaagtaagta ctaaactact
     6721 ttataatagc gactcaccga taatacgaac ttctggttcc aaatggacac ctgaagttga
     6781 ttcaacggtg ttaataacat gttcaataag attttcataa tcttgagctg aaccttctgc
     6841 aatgttaacc ataaaaccag catgtttttg actaacctct actccaccaa tacgttgacc
     6901 tttaagatgt gcttcgctaa tgagttgccc tgcaaaatgt cctggaggac gtttgaacac
     6961 agaaccacaa gaagggtatt ctaaaggttg tttcagctca cggagatagg ttaaacgatc
     7021 catttcctga gtaatcatga gatgatctcc tggttttaag gcaaacttag ctgatataac
     7081 aatatctcca ctctcttgaa tgacagaatg acgataacca aattgcatat tcctagcttc
     7141 aatcgttttg agttcaccct gtggtgtcaa aacttgtgca gacaataaaa tatgagcaat
     7201 ttcaccaccg taagcgccag cattcataaa gactgctcca cctacacttc caggaattcc
     7261 gcaagcaaac tcaaagccag ttaaactatg ataacgtgca atacgagttg tttcaatgag
     7321 attagctcct gcttctgctt caataacata accgttgact gtaacagttg acagtttatc
     7381 aaacatgata acaaaaccac ggataccacc atctctaacg ataatattac tcgcatttcc
     7441 taaaaccatc caaggaatat tttgactgtt agcaaactta acaatacgcg atagttcaag
     7501 gcgattcctt gggaatgcaa gataatcagc aggtcctcct actttagtat aagtatattt
     7561 ctttaaaggc tcatcaaagc gaatatcgag accttctaat tctttttgta ttgttttaat
     7621 cataaccttt atctcaattc tgttttattg tgttttatag acacaaaaaa tcggcataaa
     7681 accgatttct attataccaa aaatataggt tgttgacgat acattatcac aatttctgtg
     7741 tattttaatt ttaaaatcct aactgactat tcgactggct tgaaataatg aacttctcct
     7801 aaattcaatt tttcgagata gtcttctagc ttagaaccat taggtaaact tagttgaggt
     7861 gctatttcga gtaacggaac taaaacaaat gctcgcttcg tcatatacgg atggggtact
     7921 actaaatctt cttggttaat cacttgatta ccaaaaagta agatatcaat atctattgtc
     7981 cttggacccc agtgctcatg tctcacgcgc tttaagcgtt tttcgatttc ttgacaataa
     8041 tccaataatt caaaaggtgc taagtctgtc tctacctgac aacatatatt aaaaaaatct
     8101 tcttggttag tattccccca agcagctgtt tcataaatag cggaaatttg cgcgactttt
     8161 gtcttttgca aatgatcaat tgaaaagaga gcctgcttca gaaaagtttc tctatcacca
     8221 atattactac ctaaactcaa gtaaacggtt gtcatgctcg cttgcgctcc aattcaattc
     8281 caactgattc atagtgccca ttaattggtg gattgtcttt tgtaatcttt aatgtaatag
     8341 cttgtactgg atgaaactgt aagaaaatat cttcaacaat aacacctgct aatttctcta
     8401 ttaattgata ctgttcgttt tcaacatgat ttttaataca gtcaaaaact tcaccataat
     8461 gaaccgtatc aattaaatca tcagtttgtg atgcttttgc caaatcaagt gatagaacag
     8521 catcaacttg aaaaacttgc cccaatgttt gttcttccga aaaagcacca tgataaccat
     8581 aaaaccgaca tttatttaaa taaatcttat ccatattaca ccaactgact aattactgtc
     8641 acaatgtcct tatttacttc aacattgtga acacgtacaa tctgacaccc ctttgctatt
     8701 gcccatgccg ataaagcagc agtccccata tcacgttcag ttacttccct attaccacct
     8761 agaagataat taacagtacg ttttcgagag atcccaaata acacaggata acctaaatca
     8821 cacacttgtt ccaaaccttt taatagttct aaattttgct cctctgtttt agcaaaacca
     8881 aagccaggat ctatccaaat attatccttt gaaacacctg cttctaaagc acgctctgct
     8941 ctttcaagta agaattcaca aacttctttt ttgatatctt gataaacagc ttcttcctgg
     9001 ttatgcatca aaataattgg aacatttcgt tctgctgcta aagatagcat tttaccatca
     9061 tataaacctg accaaacgtc atttaaaata tgtacacctg catcaagggc tgcttgagct
     9121 acctctgttt tataagtatc tattgataac aaaagatcag ggtagacctc tctaatagct
     9181 ttaatcattg gaactacacg tttaatttct tcttcagcag tcacaaatac tgcacctggc
     9241 cgcgttgatt ctcccccaat atctacaatt gccacccctt gttcaagcat ctcaccaact
     9301 tgatttaaag ctgaatcaat tgtggtataa gaaccaccat ctgagaagga atcaggcgtt
     9361 acatttaaaa tccccatgat acaagcttta cctgtaatat catattgtcc tattttcatc
     9421 ttttatcctt tttgtattag cgatagaatt tcttgtcttt catatcgatc gtccttgtat
     9481 aaaccacgag ctaccgttgt aatcgtctta cttcctggtt ttttaatgcc acgcattgtc
     9541 atgcacatat gctctgcttc tatcataaca aaaatacctt taggcgctaa agcatcctct
     9601 aaagcttgtg caacttgtgc tgttaaacgt tcctgaagtt gaggacgctt actcgccact
     9661 tcaaatgcac gtgcaagctt actaagtcca gtaacacgtc catcgtttgg taaataagca
     9721 atatgagctt taccataaaa aggaaccaaa tgatgttcac acatggaata gaatggaata
     9781 tccttgacaa tcacaacctc ttcatgattt tcagaaaaga ctgcagtgaa ttgctccttt
     9841 ggatcttgat taagtcctga gaacatttct atatacattt ttgccacacg cttcggtgta
     9901 tctttcaatc cttctcgatc aggattttct cctaaagcct ctaaaaattg ataaatagct
     9961 ttttccattt tttcttgatt gggcattaat ttatcctatc ctttttaagt tgttgtagtt
    10021 ttgattaata gtagttcctg acgaacttga gaaataaaat aaagggagcc agtaatcaca
    10081 tagaaatctt tatctgatgt cagatttata tcatgtagcc atttcttcca atcactaact
    10141 ctagtgtacg ctttaggata tttgtctaag tttattgatt tagggtaatc aaagctagta
    10201 accgaaaccg gagcgatact agacaatgat tctaacatac tctctatcgg tttagtatta
    10261 attgcagcaa atagaatatg cacgtttttc tgctgatatt tttttataac ttgaacgaga
    10321 gcatcgacac tttcattatt atgagcgcca tcaatcatta catttggaaa gattaattct
    10381 gtacggccta cccattttgt catttcaagc ccatctttaa tggtctgtag ggttaacttt
    10441 ggatatttgt ctcttaataa taaactggta gttatagcta tgctagcatt ggaaacttgg
    10501 tgatgtcccg gcatttgcaa ctgaatatga tcaatatttg cttgaggacc tatatagtta
    10561 aaataatttt cctcttcttt tatataaaag tcattgtgta aacgataagt ctggctgtgg
    10621 gtttccttgg cttttatttg aaagacttct tcaacatcct gacgatcatt agcatatacg
    10681 aatggaactc ctttttttaa aacacctact ttttgttcag ctatatctac ataagttcgt
    10741 cctaaaacct cctgatgatc taatccaatt gacggacaag taaccgctaa agccttgaac
    10801 atgttggtag aatcataata accgcccatt ccagcctcaa taatgacgat atcaactgga
    10861 cagctattgc caaaataata gaacatcaaa actgttataa cttcaaattc tgtagctggt
    10921 tctaggttgg tctcaaggtg caaccgttca acaactggac gaacagtttc aacaagtttg
    10981 ataaaatcag actctggaat catttgacca tcaatgctaa tacgctctct aaagtcaaca
    11041 atatatggtg atgtaaatgt tccaacttga taaccagaat ttgtaaaaat atgttgtaaa
    11101 taactagttg ttgatccttt accattcgtt ccaacaacat gaatggcaga taggttgttt
    11161 tgagggtttc ctaactgctc tagcatccat ctcatacgtt ccaaacctgg tttaatacca
    11221 aaagctaatt tgctatgaat ccattctagc gcttcttgat aagtcatctt atttaccttt
    11281 ctttaacatc gtatgtactt gtccaatact aaaaactaaa atagcaagcc agataaaaat
    11341 aaaacctatt acctccccac taacgatagt ttctttaaaa ataaacaatg ctagcaataa
    11401 ttggatggtt ggattgatgt attggataaa accgataata tttagaggag ccctcttaat
    11461 ggcttctgca aataataaaa gaggaactgc tgtaatgatt cctgaaagtg agagtaaaac
    11521 taattgtaat atattataat cagttaaaaa atctttggcg aaaaacacta tataaatgag
    11581 tgcaaacgga gcaataaaac tactttctac cagcatcgaa aaatcgctag ataaagaaat
    11641 tgattttttt aataacccat aaaaaccaaa agaaagcgct agagttagtg aaattaaagg
    11701 aaaatgacca gtttgataaa ctaaaattcc aactcccatg attgcaatca aaattgcaag
    11761 gctaactact cttgccaggt gttcacgtaa aacaaggacc gacaacaaaa ttgaaataat
    11821 cggcatcata taataaccaa gacttgcctc agtagcgtga ccatgcgtga ctgcaaaaat
    11881 atagaccaac caattaattg aaattaaaat actggctaca aacattgtcc aaaatgactt
    11941 tttattatta actaagcctt ttaagtcttt taaatagact gtttttctac ccgaaacgag
    12001 catataaata agcatagtta aaacagtgaa tataatccta taagcaaacg tactatatgc
    12061 ttcaattcca gataataatt tccaataaag agataacagc ccccataaaa cataggctga
    12121 caagccaagg ataattccta aattatcttt tctcaactct cactcctttt cgctcaatat
    12181 taagtacttg aatctgtcca tctaaacata atttacttaa ctctgtgaca attggttgtt
    12241 ctttttcttt cgaacacaga accattaccg tcggtcctgc tccagacaaa taagtagcat
    12301 acgcaccgtt ttgagtgctg atttgtttta taactccaaa ttccttcacc aaaggttgac
    12361 gatatctttc atgaaagagg tccctttcta tagcccatcc agcagttacc aaatcacctt
    12421 tcaacaacga cgcaaccatc acattagcga cgctgcttga agcaactgca tcctcataac
    12481 tcaatttttg aggtagtaca tttcgactat cacttgtttt taattcataa tttggaataa
    12541 aagcgataaa ggaactatct ggaaacatca cttccaaact tttaatatca tttttcacta
    12601 ttgatgaaat aactaaatca ccaaaaatgg ccggtgctac attatcagga tgtccttcta
    12661 atcgggtagc tatctctaat ttttcttttt gagaaagagc caaatttcct aattgattgg
    12721 ctaattctat tcctgcaaca attacagagg aagaagaacc taaacctctg gccaaaggaa
    12781 tatctgaaaa catttttaag cggtgtggtg ccaaatccga cttaacatgc aaagctgttt
    12841 gaataaggag gttagtatga tcttttggaa tatttactaa attgtgttca actaaccact
    12901 ctgtactttc ttctaatact tcaatgctta aatactttga taacgcaact cctattgaat
    12961 caaagcctgg accaatattc gcactagttg caggtacaat tatcctcatt tcaccctcct
    13021 attactttga aaacattgat taactgaatg acaccactct caggaaactc ctcaataaat
    13081 tgagttattt tctcttggct aacttcatct gttaatacat agatttcttg ttgtttatca
    13141 tccaatgctt tttcaataac atttttcaaa cctatatttt cttgctcaaa gtaccttcca
    13201 agtgtatcac tatcttcagc atttctcaaa gaaatagcta gatagtactc attgaagata
    13261 tcagactgca ttgacaacct acacggacaa tgataagtat tcataggctt aatttttatc
    13321 tgtgatcgaa tactacgact aatatcgatg atatcggcta aaacagaggt tgctgttgga
    13381 ttttgtcccg cacctggtcc ataaaaaaga gactgaccaa tccctattga ttcaatgaat
    13441 actgcattca tcacatgatt aactgcagct aagggatgct cttgagaaat tagtgtcgga
    13501 gaaacgtcta cacttatacc agttggagtt tcacgaagat cagctaccaa cttaatggca
    13561 aagcccatct cctgagctac ttcaacgtcc tctgagcgaa tactagaaat ccccttataa
    13621 ttaacatcat caaaatcaat aggcatacca aatccaaatt gacttaagat agtggctttg
    13681 taggcagtat caataccttc aacatcattt gtaggatcac tttcggcata acctaactct
    13741 tgtgcctttt ttagagcttg ttcataagac catccctctt caaacatttt tgttaacatg
    13801 aagttggagg taccgttgag aattcctaat aaacgtgtca ttttatctga ggcaaacgaa
    13861 tgcgataggg tccttaaaat aggaatgcct ccagcaactg ctccctcata gaataaagct
    13921 agacctctag cgtctgctaa atccatcaac tcttttccgt gtaaagcaat gagatcttta
    13981 ttggctgtca caatatgttt accaaatcct aatgcttgac tcaaataagt tttagctggc
    14041 tcaatccccc ccattaattc cactacaata tcaatttgtg gatcttgaag aatagtattt
    14101 atctctgtca caaagttata agtaaatccc tgattgatga aacgctggcg gctttcatta
    14161 tctcttacta atactttatc aatcacaata tcttcgcctt ctaaagaaag tagcttatgt
    14221 tgattttctt ttagcaaata tggaataccc ttagcaaccg ttccaaaacc taataaagct
    14281 attttaatag tcatgaattt ctccttagaa attgtctcta acttcaagac aaacattggc
    14341 tatattataa caaattacca atatttttta tagggtaaac gcgtcttgtc ctatgaaagt
    14401 tcttaaaaag agaatattat ttcagaaaag atgatacaat aaaagtactt taaaagttaa
    14461 ggagaaataa atggcacaca cacctactag tcatcgtaaa ccacgaaaac gttccccatg
    14521 gttagctatt gcttctgtct tttttttgtt aatagcttta atcggcatat ttctattctt
    14581 caacaatcgt tcaaagcaag aaataaaaac aaaaacgaac gcctctagtc atcgaaaaat
    14641 tgtcacatct atcaaaaaaa agaaatgggt taagcaaaag actcctgtta aaatacccat
    14701 tttaatgtac catgctgttc atgttatgga cccttctgaa gccgccagtg ctaacttaat
    14761 tgtggcacct gacatttttg aaagtcatat aaaacgtctc aaaaaggaag gatattactt
    14821 cttagcccct aacgaggcat atcgtgcact aaacgaaaat gctttacctg aaaaaaaagt
    14881 catttggatt acttttgatg atggaaatgc cgatttctac actaaggcat accctatact
    14941 caaaaagtac aaggttaaag ccactaataa tatcatcact ggctttgttc aagaaggacg
    15001 agaatctaac ctcaacgttc aacaaatgct agaaatgaag caaaacggca tgtcattcca
    15061 aggacatacg gttactcacc ccaatctttc tctcttaact ccagaactac aaacccaaga
    15121 aatgacacta tcaaaactgt ttttagatca aaaactctct caagatactc ttgcgattgc
    15181 ttatccatca ggacgctaca atcctacaac tttggatatt gctagtcaat attataaact
    15241 agggcttaca acaaacgaag gagttgcaac aaaagataac ggtttgcttt ctcttaatcg
    15301 agttcgtatc ttaccaacta ctagcgatga tgatttgatc aaaacgatta accaataaaa
    15361 agaagccttc tggcttcttt ttattcttca taagatttaa atggttcatt tttacgtgga
    15421 taacgatctg gagaaatcga taagcccatt tcttttttcc catggaaaag ctccactaat
    15481 agcgctagca tcatcaaaat cattacgaat gaaaatggca gagcgacaat aattaaaacg
    15541 ttctgcagcg ctaacagacc accagacaag agtaagacaa tagcaatcac tgccaacact
    15601 agcccccaaa ttactttagt tcgatttttt ggatttaaat taccatcttc tgtcaacata
    15661 gccaaaacgt acgttgcaga atctgctgat gtaataaaaa atgaaaaaat taaaatgata
    15721 gcaatagtgg ataaaagcca acctaaagtg tagtgattaa aagtagcaaa caatacttcc
    15781 tctgttgcaa actttgtcaa attaataccc agttgttgta cctgagttga taatgtgcca
    15841 aatgctgcaa accatacaaa actcaataat gtcggtatta aaagaactac cgttaaaaac
    15901 tcccgaatgc tacgcccttt agaaatacga gcgatgaaaa ctccaacaaa aggagaccag
    15961 gaaatccacc acgcccaata aaaaatcgtc caattatcaa tccaagaacg tttagtattg
    16021 tcaaaagcag ctgcacgaaa gctcattcca aagaaatttt gtaaataaga gcctaaagac
    16081 tctgttaggg tatcaaaaat acgaaccgtt ggtcccaata caataactaa agctaagagg
    16141 gctaccgcta aaatcaaatt taagttcgat aaaattttaa caccttttcc taaacctgat
    16201 aaagctgaca taacaaataa agctgttgta ataaggataa taacaatttg aacaaatgca
    16261 ttattgggaa cacccaataa aaaacttaac cctccgttga tttgagcagc tccaaatcca
    16321 agtgtcgtag caactccaat aactgtagca acaactgtgg tgatatcaac aatttttcct
    16381 agtcaaccat ctgtcttatc accgaaaaga ggctttaaag tgacagacaa gaggtatttc
    16441 tcttgctttc gaaatccaaa ataagctaga gccaaagcaa ccaatgcata tactgcccaa
    16501 gcatgtatcc cccagtgaaa aaatgtaaaa cgaaatgcat cagctaatgc tgtttgcgat
    16561 tccttaggtg cgccaggtgt cgaaatagca aaatgcgatg agggttcagc tgctccgtag
    16621 aagaccaaac caatccccat acctgctgaa aacatcattg ctatccatga tacagttgaa
    16681 tattcaggac gtgaatgagg tcgccctaaa cgtatttgtc caagaggact cataataaaa
    16741 aagacacaca aagcaagaat tgaagtaact aacaacaagt acaaccaacc gaaattggta
    16801 gatacctgtt cacgcaagat ttgtgtccaa gattgaaaac cacgaggaac aataattcct
    16861 aataagacta aaataagcga tacaattagt gaacctgtaa acacaggcgt aatatgtttt
    16921 ttactcaaaa tttcctccta tactaaaata gtatagcata ctttaataaa tactcctctc
    16981 attttaatca cctgtatcaa gacctctcct tgtctcctca acaaaaaaga accagataac
    17041 attatctggt tcttgtgttt gacttagtga ttaatcacta tttggtgtaa ataccaaatg
    17101 ttcgttgaca aaggcatcaa atccaagttg gctcaattca cgaccatatc ctgaattttt
    17161 aatacctcca aacggtaatt ccggtaatga tgtccaccct gaattaataa atgtcatccc
    17221 tgtttcaatc tgagccgcca cttttttagc atgttctgga tcagaagaga aaacagtgct
    17281 tcctaaacca taactagaat cattagctaa agcaatagct tcttcttcag tatccacttt
    17341 atagatagag gctacggggc cgaaaatttc ttgattatag attggatttg ctttagtgat
    17401 attcgttaaa acagttggca tgacgaagtt tcctggatga tctatagtgt catttccaaa
    17461 aactacctca gcaccgtggt ctaccgccaa tttaatttgt tttaaaacat catcctttgc
    17521 acctgcagat gacagtggag ccaaggttgt ttccgaatcc attgggtcac cccatttagc
    17581 agacttaaag gtctcaacta ccatatttac aaattcgtca tacttatcag ccataacaat
    17641 gaaacgtttt gaagatgtac atacttgacc tgcattatat aaacgcgcaa agaatattgt
    17701 tttactaagt aaatcaaaat cagcatcatc taaaataaga aaagcatcgt tcccacctaa
    17761 ttccatagac gattttttca aatttttacc agcctcagct gctattgatg ccccaccacg
    17821 ctcagaacca gttaaacaca cacctgcaac acgaggatct gatataaggt ttgaaacttg
    17881 atcatgcgaa gcaaaaatat ttttaaacgc tccttctgga gctcctgctt ctcggactaa
    17941 atcctcaaat gcttgagctg atgcaggaca aatagaagca tgttttaaaa gcatagtatt
    18001 acccactata aagttagggg caaatacacg cataatctga taaaatggga aattccatgg
    18061 ctcgacagca agtaaaacac cgacagcttg tttcaaatag taagcctctc catttggact
    18121 ttctaacgga acaggtttta aaaacttttg cccattatca gcataatagt cagctatgtc
    18181 tgcacacaag tccacttctc cttgtgcttc agtgaaaagt tttcccatat ctttagtcat
    18241 aacttcagcg tacttatcac ggtccttacg taataaatct gctactttat gcaattgatt
    18301 ttggcgttct tcaacattat cttctttgcg ccatgtcttg tagagggcat gcgctatgtc
    18361 taatgactgt tcgagatcac tgtcactgat attatcgaat tcatgcaata cttcgttagt
    18421 gtacggataa attgttttat atgccataat gttccctcct tttgatatcc tcattataac
    18481 atttatgaaa tcgattacaa atatttagct ttagacaaga tcaccaagtt ccaaggatag
    18541 cttgcattgt taaactaact ataataatgc ctaaccaaca cgctgcacct aaaagaattg
    18601 attttcctcc agttttaatt agtttgctga cattagtatt tataccaatt gcccccattg
    18661 ccataacaat aaaaaattta gataaaacct taagtggctc aaagatacgt aacgaaaaac
    18721 ctaatgaagc aactatcgtt gttagcaaag aagctaagat aaaataaagc acaaacttcg
    18781 gaaatatctt agtaacactt acagattgtt ctttctgcgt ttgtttcata tgataaatag
    18841 ataatacaat cgtaattgga atgatggcta atgttcgtgt caatttaaca atagtagctc
    18901 cgcccaaagt attgctgtga ttgatagcat cccatgcagt agcagttgct gtaactgacg
    18961 aggtatcatt gacagcagtc cctgcaaata atgcaaaacc gtgatcagag agaccaataa
    19021 aattccccag cgttggaaaa attaaagccg ctaagatatt gaataaaaaa ataaccgaaa
    19081 tagcttgtgc tacttcatca tctttagcat taataactgg tgcagtcgct gcaattgcgg
    19141 atccaccaca aatagatgaa ccaacaccta ttaaagtagc aatcgtttta tctaacttaa
    19201 ataacttttg caaaacataa gcaataatca atgaaatgga aatcgtcata ataatgatgg
    19261 gtaacgatga tataccaact ttcatcactt gcatgaggtt tagtccaaaa cccaataaga
    19321 caactgctgt ttgtaaaata tatttagacg taaaagcaat acccttatta aacaattgtc
    19381 gattttgata aaaggatcca acaattattc caatcaagat agcaaagact ggtgctccta
    19441 ttaaagggag ataaagccca agcaaccaag atggtatagc aataataaag cataatatta
    19501 gtccaggaat tttttcctta aataacatga tagtcctcct cacattgact attatatcac
    19561 aagctttaaa acgtggcagg aaaaccgaaa gaggaccaga atatgctctg atcctctttc
    19621 attactaatt cacaattatt tagttacttt cataaatgga tatgattttt cgttacctaa
    19681 aatcaatttt gagccctctt ttttataggc tgttttttca ccatttttca caatattacc
    19741 atagaacgca tttgcctttt tattgatatg cgatacttta ataacacttg ttaagcgatg
    19801 tactttacca tccataactg gcaatgtcat ttcaccagta ttagtattat attttcctcc
    19861 gacagcttgt gcagcacttt ggaaacttct atcaatttgt tggtcaattt gtttgtcagt
    19921 gatttttgtt tcatcaaaaa tctttttacc tgcttcactt aaatcattgt aagtcaaacc
    19981 ttgatctttt aattgttttt caagagcttt atcaataaaa gtttttatag cttgatggaa
    20041 ttttacctca tcaatttgag cagtgactgt tatctttgcc tcatcgtttt tgacgttcat
    20101 atgcatatta gcgcccttaa ccaaatcagc tgcactaatc cccatatccg ctacctgact
    20161 tccaagttgt tgttctattt ctttctcaac ttgcttctct aaagaaactg tacgccattt
    20221 cccctctagt gactgacgat tattccagaa atagagaccg cctgcaccta caattagaat
    20281 aagtactagt acagttaaaa aaacttttaa aaactttttc atgataaaaa ctccttatga
    20341 taaactacat taatcataac ataatatcac aactccttca attaaaatag taaaataatt
    20401 atataaaagt ttgtaatttt atacatttta atgaaacc
//
LOCUS       DATRSD010000039        19064 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRSD010000039 DATRSD010000000
VERSION     DATRSD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19064
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.039"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(120..737)
                     /gene="nrdG"
                     /locus_tag="VBD85_001110"
     CDS             complement(120..737)
                     /gene="nrdG"
                     /locus_tag="VBD85_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO7133670.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDDLL"
     gene            complement(810..1301)
                     /locus_tag="VBD85_001111"
     CDS             complement(810..1301)
                     /locus_tag="VBD85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133671.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTMHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGVLEDCRHQTERY
                     WIT"
     gene            complement(1310..2242)
                     /locus_tag="VBD85_001112"
     CDS             complement(1310..2242)
                     /locus_tag="VBD85_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7133672.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(2255..2398)
                     /locus_tag="VBD85_001113"
     CDS             complement(2255..2398)
                     /locus_tag="VBD85_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133673.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(2473..4671)
                     /gene="nrdD"
                     /locus_tag="VBD85_001114"
     CDS             complement(2473..4671)
                     /gene="nrdD"
                     /locus_tag="VBD85_001114"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7133674.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRIAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYDNVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(4769..6310)
                     /locus_tag="VBD85_001115"
     CDS             complement(4769..6310)
                     /locus_tag="VBD85_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133675.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLS
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(6561..6878)
                     /locus_tag="VBD85_001116"
     CDS             complement(6561..6878)
                     /locus_tag="VBD85_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO7133676.1"
                     /translation="MAHNHNHDHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLIPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(6904..7323)
                     /gene="ruvX"
                     /locus_tag="VBD85_001117"
     CDS             complement(6904..7323)
                     /gene="ruvX"
                     /locus_tag="VBD85_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO7133677.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQAYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(7323..7589)
                     /locus_tag="VBD85_001118"
     CDS             complement(7323..7589)
                     /locus_tag="VBD85_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO7133678.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(7791..8189)
                     /gene="spx"
                     /locus_tag="VBD85_001119"
     CDS             complement(7791..8189)
                     /gene="spx"
                     /locus_tag="VBD85_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO7133679.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(8405..9544)
                     /gene="recA"
                     /locus_tag="VBD85_001120"
     CDS             complement(8405..9544)
                     /gene="recA"
                     /locus_tag="VBD85_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO7133680.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(9618..10877)
                     /locus_tag="VBD85_001121"
     CDS             complement(9618..10877)
                     /locus_tag="VBD85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO7133681.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQLVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELNPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKVGEVTL
                     RLSTKAENQDEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVI
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(10966..11517)
                     /locus_tag="VBD85_001122"
     CDS             complement(10966..11517)
                     /locus_tag="VBD85_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO7133682.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRYIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(11540..12130)
                     /gene="ruvA"
                     /locus_tag="VBD85_001123"
     CDS             complement(11540..12130)
                     /gene="ruvA"
                     /locus_tag="VBD85_001123"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO7133683.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK"
     gene            complement(12132..13364)
                     /locus_tag="VBD85_001124"
     CDS             complement(12132..13364)
                     /locus_tag="VBD85_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7133684.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYFPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCFVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSILGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(13396..15369)
                     /gene="mutL"
                     /locus_tag="VBD85_001125"
     CDS             complement(13396..15369)
                     /gene="mutL"
                     /locus_tag="VBD85_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO7133685.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQPGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            15595..15798
                     /locus_tag="VBD85_001126"
     CDS             15595..15798
                     /locus_tag="VBD85_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO7133686.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(15855..18431)
                     /gene="mutS"
                     /locus_tag="VBD85_001127"
     CDS             complement(15855..18431)
                     /gene="mutS"
                     /locus_tag="VBD85_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO7133687.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIVVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEVLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(18488..18925)
                     /gene="argR"
                     /locus_tag="VBD85_001128"
     CDS             complement(18488..18925)
                     /gene="argR"
                     /locus_tag="VBD85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7133688.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
BASE COUNT         6012 a         3879 c         2874 g         6299 t
ORIGIN      
        1 aatacttgtt aaattaacaa taataaacgt caacatcttg tcacagactg tcaaatactt
       61 ataaacaagc tgtggataaa cataactttc aatttctatt taaaagttaa tttttgacac
      121 taaagtaaat catctcgttt gacttgttca taactattgt caccatcgtt taatccttcc
      181 caaatcacga cttcaccttc ttttaaagat ttttgtacat caatgattct ttgatttgaa
      241 gatcctctaa attgtagcat taaatttcgc ttactttgat caaacctacc atccactaaa
      301 atatcaatca aagataacat ctccaattta tcttgagtct ccagcatcat ttcttcccaa
      361 gtataccccg tccatgacca aatatctttt tctggcaatt cacgtcggat acgctgaaga
      421 agtggcagca aaatacctgt atttaagaac ggctctccac ctaataacgt taaaccttgg
      481 acgtaaggtt gtgccaaatc tttcataatt tgatcttcaa gctcttttgt ataagggatt
      541 ccggctcgaa aggaccatgt agcagtatta taacaccctt tacagtggaa catacatcca
      601 gctacgtaga gtgaatttct aacaccttca ccatctacaa aattaaaagc tttataatca
      661 atgatatggc catgacttaa ttcttcgctt ttccactccc caggctttgg agtattccaa
      721 cttgcttctt ctgacatttc taacctctct attgatatga aaagaaccag aactaactgg
      781 tctttttatt attatcttaa agtactattt taggttatcc aataacgctc tgtctgatga
      841 cgacaatctt ctaaaacacc accgttagct aatattactg acttgctggc atcattgtct
      901 acatggcatg tcacaagtat ttcattgata ttttttagat gtgcttgctc aattcctagc
      961 ttcaactgtt ctttagcata acctttgcca cgctggcgag gtcgtatgga ataaccaata
     1021 tgtccacctt tttctaaaag agtatcattt agtcttaaac gtaaatttaa gaaacctata
     1081 gcattattat cattgtcaaa agacacaaac tgaatatatg gaacaaaatt atcaggaaca
     1141 cctatcccta actccatatt taggttatga tctagccagt cttcaaaatg catagttggt
     1201 tcataaaaac caccatccat tgaactactt tctttttgaa actctttata catggctaaa
     1261 atttcttctt tatcttctaa cactgggcgc cgtaatttca tattgtttcc tattccatta
     1321 tcttcaagtt aagcattcca aaaggagcat tcgctttatt aatctggtca caaataaaat
     1381 gactcaataa actagacttt ggtgatatag gattgacacc tgtagtgata gcttgaataa
     1441 aagcatcaat cattggttca aaaccacgtt tctctagagt gcttgcccaa gagccaaaac
     1501 cgatagcacg acgatcaaag ccatcaataa cagaaagatt tgacaaatca tctaatgaat
     1561 aggttacttc tggcgactct atttcaatta tttctcgacg agaccccgac tgcaggttca
     1621 tgcttgcttc tattatctca ctgtctgttt ctagcgttac tgatacttgt aatatcttat
     1681 tcccatcacg tttagtcaca aagtatcctc ttttaacaac attatttgtc aaaaatagcg
     1741 ccgtatctaa aggatggata aacatatcaa aaagcttata ttgaaaatcc gctggtgcat
     1801 tgacagcatt tttaaaggtg cgaatatggt ttttatcttc tacttttttc atttccataa
     1861 tacgtggtgc aaagcgacga ttaaaacctg ccattaaaaa tgttttatga tccttcgcca
     1921 aatcatacaa tgctttagtt gaagtataat cttctgtcaa aggcttatcc ataaaaactg
     1981 gaataccctt ttttaaaaaa agctttgcta tatcaaagtg agcactagtc gcgacatgaa
     2041 tgaagactcc atccaaagga tgctcggcta gactatctaa actgtcataa gctgtagaag
     2101 aaccaaaaag catattaact tcttctaaaa tctgtttttg gcgtgtaaaa agatgccagt
     2161 gcacacctgt cacttgacgc atataaggta gataagcttt ctgtgaaata cctcctaaac
     2221 cgacaattcc aagttgcaac atagcatcct ccttctattt tttctttgcc ttttccaaaa
     2281 attgtttcct taaatctttt agacgtgatt tttgattagc attctctcca gtagagtgct
     2341 tctcatggaa cttctgtact tgtgcttgcc ctttgtaatc taattggtat ttccccatat
     2401 cttaataact ccttctattt tatcttctta ttttaacata taatagacca gaggttttaa
     2461 tagaggtttt aattaattac cttgattttt aatacttgat ccgttcatat gttttacacg
     2521 agcagagatt tccttatgac gaccattaac catcggacga gcctgtggat tacctaagta
     2581 accacatgta cgtttgacaa catctactgt cttagggtcg ctgttaccac aatttggaca
     2641 agtaaagcct cgatctgtcg gtgtaaaatc cccttcaaat tgacattggt aacacttatc
     2701 aattggtgta ttagtaccta gataacctac ccgatcataa gcatagtccc aaactgcttc
     2761 taaagctttt ggattctgtt gcaaaacagg gtactcacag tagtgaataa atcctcctga
     2821 tgcacccgtt tcaggataga ttttctcaaa atctagtttt tcaaaaggtg ttggattctt
     2881 acgcacatca tagtggaatg aattagtgta gtattcctta tcagtaatat cagttactat
     2941 accaaatttt tcagtatcta aacgacagaa ccggtcagtc aagctctccg atggtgtcga
     3001 ataaactgaa aaatgataat catactcatc agaccaatct gcacataact gcttcatctt
     3061 acgaacgatg tctacagtaa atgctttagc ttgatgatta tcttcccagt caccaccata
     3121 aaaaacactc gccacctcat ataaaccaat ataaccaaga gacaccgttg cacggcggtg
     3181 attaaagagt cgatcaacat tgtcatattt tcctaaacgt tctccaaatg ctccatactg
     3241 gtaaagaatg ggcgcatttg ctgggatagc ctctttgaca cgttccacac gataaaccaa
     3301 ggcatctcta gcaatactca tacgttcatt aaatatctcc cagaatttat ccatatcacc
     3361 gttagactcc atggcaatgc gcggtagatt aactgttaca actccaagat tcatacgacc
     3421 agatgttaca tcctgaccat ttgcatctcg ccacccttgt aggaaagaac gacagcccat
     3481 aggggcttta aaagagcccg ttaagtcaat aattttatca taagaaagga catctggata
     3541 catccgtttt gtcgcacact ctagtgctaa ttgtttaata tcataattgg ggctattttc
     3601 ttctaaatta aggccttttt taagcgtaaa aattaatttt ggaaaaatag cagtacgatg
     3661 ctcgcttcct aaaccttgaa tccgaatttt caaaatcgct ttttgaattt ctcgttcaaa
     3721 ccaattagtt cctaatccaa aaccaagcga agtaaatggc gtttgcccat ttgaagtaaa
     3781 taaagtatta atttcatact ccaaagattg catggcatca tatatatctt tttgtgtttt
     3841 agcgcgcgca tagtcttctt gtttatcttc aataacccat tcttttgcat cttttaaatg
     3901 tttttgataa tttaactgtg cataaggagc taaaaactca tcaatgcgat ctgctgtaca
     3961 gccaccatat tgacttgaag caacgttcgc aataatctgt gaaatctgag cagtcgctgt
     4021 ctgaatagat tttggactct caacttctgc attaccaatc ttaaaaccat ttgcaagcat
     4081 tcccttaaaa tcgattaaac agcaatttgt catgggagtg tatgggctgt aatctaaatc
     4141 atgataatgg atatcaccct tttgatgtgc atttgctaca tgagaaggta acattttcag
     4201 accaattgat ttccctacaa tacctgctgt taaatcacgt tgggtattaa acacatcaga
     4261 gtctttatta gcattctcat taacaacagt ttgatcctta ttaattaatt ttccaataga
     4321 aaaattgata tctgtagcct gtgaacgctc aaaatcgcgt tgggtacgat aattgatata
     4381 ttcttgtgca atagcgtact cattagcttc caataattta tgttcaacga tattttgtat
     4441 ttcatatatc ttgatgttat ctttaaaacg attgtagatc tcagcgatga catgatctgc
     4501 aatcatctca agtttagcct caactaaagg agacattttg ataactttat tactagcttt
     4561 taatagagcc gtgtagattt tatacttatc aaagctgacc aaacgcccgt cacgtttaat
     4621 cactttgata tcagattctg tcataaatcg ttctctttcc aataccatca tgtgaaatat
     4681 cctccagtga caattatagc atgcaaagaa aaaaaatcaa tatattgtgt caagtttttt
     4741 gctcagacac aatatattga tttttaatct atttgaaacg atatagaaca aaatgagtga
     4801 tatttgatag aggaacttct tggtagtgct tcttaatatc agatttcagt tcatttggta
     4861 acttctcatt tttatttaag ataaagtatt tagccccatg ctgaagcagc tcgtccttca
     4921 aaattttctc attatttttt tgcgctgtat taatatgtgg caaaacaaat tgagaagcag
     4981 attttaaccg agtctggcta taaatgctag caactttgtc ccaaacataa atcttaccat
     5041 ctctatcaat ttttgtagtc aaataatgtg aaatgtcacg cctttcttta ctaagagtaa
     5101 cattttgata agtattataa actagtaacc ctatcgataa agaaactagt aagaggggca
     5161 aataatatgt tttctttaca aaaaggtcta cgataagaga tttaccagag aaatactgac
     5221 gtctatgtga acgcctgtca atcacttttt ggtaaagaat agtaatttta ttactcgtta
     5281 gcaaaagacc aaatggtaaa gcaggtaaaa agtggtaaag gttaaaatct cttgaaaaaa
     5341 tggccattat agcgtaaata ataaaaatac aggcaataaa gttcaagccg atttgataag
     5401 atgctttttt acgattttga ataaaatccc atagtccaaa gagacaacct gaccctagaa
     5461 ggacaacaat ttgaattgct aaactaagaa ggaatgagtg gtttgtccta gcgaaatacg
     5521 taaaggggta aaaaatagct ttgtcaatat aagaagagat aatctgtaga ttaaacataa
     5581 agtaaccaac agtataagca attaaaatca taccaaaaac aacgcataaa aactgataaa
     5641 atcctcgtag tgactgcttt tgcttaatat tggttgctgt taagagaaca aaagagcaaa
     5701 gccaaaaaat cagtgtttgg ggatctataa atagtgagaa tgctgctaaa atgccataaa
     5761 atataaatgc ttcatcaacg atattatcga cacaaaattt aattaaaaac catagcgaaa
     5821 tgagcataaa aggtaatgcc cactgtattg ggtacatacc accgaatcct aagacaatat
     5881 tcattatata gaaaatcaac gtcatgccta tcgagacaat ttcactttgt gccacataat
     5941 aaactaattt ataaaaataa attccagata catagtaagc aataaactgt acaataagta
     6001 accagatact agaccctaaa aggtaactta gagcaatcgt tacatagtat aaaaatcctc
     6061 ctgtagcaaa aacatcacta taaggaatct gtcctttggt catcatcaaa cctgtataga
     6121 gattctgagt ctgcaaacca tttgcaaaat aagtcaataa cggatttgca attgctaaga
     6181 gagttgacaa aacactatat aataaaattg aaaatcgact agatttcttc attctgatag
     6241 ccgttcgttt actcattgat gggctatttt tatagctcac tccaattggt ttttctaggt
     6301 tttctaccac tatttttctc ctcatattta catactacca gtatatcaga aaaccttatt
     6361 tagtcaattt agccaacact tgaccgtaac gaataatatc cctaaagtag tggtattttc
     6421 tagacatata ttgtttgaaa aattcttgtt cttctaatgt tagctgagta tctgacatta
     6481 acatcacctc cttttactac atcatcacta ttattattcg aaaaaaaaaa cgactgaaca
     6541 attgctcaat cgcttcaata ttattcttca tctagaaagc tgttgaaaac ttcctcaatc
     6601 atgtcccatt ctgcatctga atcttctggt ataggctgaa ggtctccttc agttccatcg
     6661 gcattttctg taaatgagta agcttgaatc tcaatttcac cttgttcgtc ttcttccgca
     6721 cctgcaggaa tgaggagaac atagtttttg ccaaactctt cacgcccatc aatagttaat
     6781 aggatttcaa aaagtgtttc gttcccattc tcatctacaa gagtgattac ttcatgttga
     6841 tgttcatggt tatgatcgtg gttatgatta tgtgccattg tttctccttt ttatgccgaa
     6901 tgcttaaaac atgcggtcta aataattttg taagattagt tgagctgcta atttatcgat
     6961 aactttttta cgcttaccac gactaatatc agcttgctca actagcatac gctctgcttg
     7021 aacagtcgtt agacgttcat cttgatattc aactggaaga ttgaataatt cagtgatttt
     7081 atcgccatat gcctgactag cctctacacg aggaccactt gtattattca tatttttagg
     7141 tagaccaact acaaatttat caactttgta ctcttttact aactctgcta agcggtcaaa
     7201 accaaaattt ccagattctt catcgatctt aatgatttca agaccttgtg cagtgaaacc
     7261 gagtggatca ctaattgcaa cgccaacagt tttcgagccg acgtcaagtc ccattattct
     7321 cattacaaat ctattccatt tccttgtaaa taataacgaa cgagctcttc tacaatctca
     7381 tcacgttcat atttacgaat ttgattgcga gcatcattgt aacgaggtac ataagcaggg
     7441 tccccactca atacgtaacc aacaatttga ttgatgggat tgtaaccttt ttcttctaat
     7501 gaacgataaa cagctgttaa tgtttcacta atttctactt tgttactatc atctaatcta
     7561 aaacgaacag tttcatctgt aaatcccata cctacacctt ctttccttta attctttcac
     7621 ttattataac ttaaataaag tatttgtaca agaaattgat aaacactata ttagaaaatc
     7681 aaaaagggag cacaagaaac caagaagtga ttctttgatt tctttactcc ctaccttata
     7741 ggtgattttt acaggttatc gtgtgactct cacttatgct tatctaaagc tcaaagtgct
     7801 gcacgaagac gagcttctgc attttcaacg ttacgaatag agcgaggtag aaaagcacga
     7861 atatcatctt ccttataacc tacttgaaga cgtttgtcat caattagaat tggactcttt
     7921 aaaatacgtg gattttcttg aataagatca ataacttcat ttacactcaa ctcctcaata
     7981 ttacaattga gcgccttagc ataacgattt ttagaagaaa cgatactttc aataccactt
     8041 tcagtttttg ttaatatttc taaaatttca tcacgtgtta gtgattcttt acctaggttt
     8101 tgttctttat atggaagttg atgagcattt agccaagttt ttgctttttt acaacttgtg
     8161 caacttgaaa tagtataaat cttaatcatg tgccttctca ctttctatcc catgatagtt
     8221 atagtttaac atagtcacaa aaatcaaaca tgggacttaa gacctatttt ttatcaattt
     8281 actaataaaa ataaatatat tttagggatt tttaagctta tttttagtat aaaaataagt
     8341 tccaaccact gtcagaactt attttagtta catttattca agttcaagat gtatagttgc
     8401 tattttattc ctcaatttca atagcattgt ccaaatctaa aacaagatca tcagcttcat
     8461 ttttttcttc aacgagctca gactgaactg gcgaatcatc ttcagtcatt ccaaagtgca
     8521 cgcgcacttt atggtcaatt tcatcaaaaa ttgctggatt atctgctaaa tattttttgg
     8581 cattttcaga accttgtcca attttctcac cattatatga gtaccatgca cctgcttttt
     8641 ggataatatc caaatcacta gcaattttaa ttaactcacc cgtgcgagaa ataccttcac
     8701 catacataat ttcgacaaaa gcttcacgga atggtggagc aactttattt ttaacaactt
     8761 tgatttttgt ctctttacca acattatgat ctttatgctc accagtcccc ttgatttgag
     8821 tattaccacg aacatctaga cgaaccgatg aataaaattt caaagcacga ccaccaggtg
     8881 ttgtttcagg gttaccaaac ataacgccta ctttttccct tagctggttg ataaaaattg
     8941 cgattgtctt tgtcttgtta atggatgctg acaatttacg cattgcttga ctcatcatac
     9001 gagcttgcaa accaacgtgg ctgtcaccaa tatcaccatc aatctcagca cgaggaacaa
     9061 gggctgcaac tgaatcgaca acaactaaat caactgcgcc tgaatcaatc aatttacctg
     9121 caatttcaag accttgctca cctgagtcag gttgagacaa gagtaactca tcaatattaa
     9181 ctccaagagc cgcagcatag gctgggtcaa gagcgtgctc agcatcaata aaggcagcaa
     9241 tcccaccttc cttttgggct tgagctacgg catgaagggc aaccgttgtt ttaccagatg
     9301 actctggtcc ataaatttcg acgatacgtc ctttaggata tccacctgct cctaaagcga
     9361 tatctaaagc aagagaacct gaactcatta cttgaacctt ttgttcagct cgctcaccta
     9421 agcgcatcac tgcgccctta ccaaaatctt tttcaatatt tttcaaagca tcatctaagg
     9481 cttttcgacg ttcatcaccg aatttcttag taatttcttc tgcttttttc gtttttttag
     9541 ccaatgtctt ctccttatat ttctatgaat ctaggttgat tcaaatttag aaatcgttct
     9601 actaattata acaaatatta tatctttaat aaagccctac gcaccaggtc aaaagcatgt
     9661 aaagtactga tatgacggac atctgagcgg ctcttccctc caatcgatac tttaatagaa
     9721 gaaacgcctt caggggttgc aatcccaata aaaactgtac ctgcagggta tccttccaac
     9781 tcatcaggac cagcaactcc cgttaaaccg ataccaaaat ctgcttttaa aagttggcga
     9841 gcttgctgag ccattgcctc agctgtaaaa tgactgataa ctccatattc ttgcaatttt
     9901 ttcttaggga tgccgagaag ttgtgatttt gcttccatac tataggtcgt aaaaccacca
     9961 ttaaaaactt gcgaagcacc agaaaattct gctaactgag attgaaataa ccctgctgtt
    10021 aagctctctg cggcagtaat cgttttattt tgtactttca ataattctaa aacagtacgt
    10081 gccatactat tattatcacc ataaccatat aataaatctt ttaatttacg gttatctaat
    10141 gtcttaagag caagtatttc tttttctaag ctatctaatt taaagtcggc ttcatcctga
    10201 ttttcagctt tcgttgataa ccttaaagtt acctcaccaa cctttgcata tggtgctata
    10261 gttggatctg tctgattctt aattatgtct tctaagattg tgactaactg actctcaccg
    10321 ataccaaaga atctcaatac acgtgaatat aattttgtac cattatttga caaaataggt
    10381 accacctgct ccataaccat tggatttaat tcactaggcg gacctggtaa aaccatatat
    10441 tggactccct tagatgtaac gataccacca acagctagac cagtaaggtt ttgtagaggt
    10501 attgagcctt caaccaattg agcttgtcgt tcattatttg gcgttctagc aaactgagga
    10561 cgacttgcaa aaaaacgatc caatttttga ctagctaata aatccactgt taatgattta
    10621 cctaaaaatt tagccaaagt ttgtttcgtt aaatcatcct ctgtaggtcc aagtccacca
    10681 cataggataa ccaattgact gcgctgacta gctatatcta aaagcgataa taaacgttcc
    10741 tcattgtctc ctacagctgt ctggaaataa atatcaattc ccaattctgc cattttctcc
    10801 gacaaaaatt gtgcgtttgt attaacgatc tgtcctgtta aaatttctgt tcctacagcg
    10861 ataatttcag ccctcacaac ttcaccctct tatctattcg aaataatgac tacattttag
    10921 caaaaaaagt agcaaaagtg ctacttaaaa catatcacat tgatattact tgaaatcaca
    10981 tatattttca tggtcattaa ccatacccgc ggcctgaata aaagaataga ggcaagttgg
    11041 accaacaaat ttaaaaccgc gtttttttaa atccttagat aagcgttcgg ataaggtagt
    11101 acttgcaggc acatcgttgt agttgttaac tgagttaacc tgtactttat tatctacaaa
    11161 agaccaaatg tagcgatcaa aacttccgaa ttctttctga attaatataa ttgacctagc
    11221 attttgtcgt gttgagaaaa gttttagacg atgacgaata accctaggat tttgtaagat
    11281 tatctctaaa tccgcatctg acattgcagc taccttttca atattatagt gatgaaaaac
    11341 ttgtctgaac tcctgacgtt tatttaatac tgtttcccaa gataaacccg attgataagt
    11401 ttctaaacat agtaactcaa agagtacatg atcgtcatga acagctctcc cccactcttt
    11461 atcatggtaa gctacatata atggattatc taaattcacc caagaacatc gtttcatgtg
    11521 aaacctcatt acttctaaat tatttcatca acatcttcaa actagatttg atgtattgct
    11581 ccacagtctc gttcgtacct tcaaaaaagg cttttacttt tttcaattca gtcgccttat
    11641 accccaacgc taacaaagct tccatagctt cttcaagatt gctattctgc tcagagctaa
    11701 ccttacgact agtcgcactc tcaccagaag cttctacaaa cttccctgac aaatcaagga
    11761 tcatctgttg tgctgttttt tttcctatct ttggaaattt agtcaaatac ttaatatcac
    11821 tattatcaat ggcacttacg agaccttcat tgtcatcgac tgcaataatt gctaaagcgg
    11881 tcgttggacc aattcctgat actgaaatta aattaagaaa aatttctttt tcattttccg
    11941 tatgaaaacc aaataaaaga tgtgcatcat cgcgaatgac ttgatgtaag tagatggtaa
    12001 cttcttgatt aacgtaacct gaaaaagagt agggatttgc cacatatatc acgtatccca
    12061 atccagctgt ttccacaaca ataaatttag ctgttatttt actcaatttt cctttaatat
    12121 aatcgtacat tctattttcc tatctcaata tttttcattt tggtagcata taagattaaa
    12181 accagacctg ctaacataaa aatggtcaat gttatttgaa cacctatttt tcctgtaaaa
    12241 tagctgagcg aaaccatggc acctgctaat acacttgcaa caggttgtgc gatagctaaa
    12301 aatccagaat atgatccaat ttttccctcc accatcatct ccgcccttag aacctgacta
    12361 gctggaacat aaatcatttc gccaaaggta aaaaacgtca tggcaatcaa aatagctcca
    12421 aaagtgttta gagagatatt aaaaagcatt ccaaaaccac aaatcaagct ccctaatatt
    12481 agttgacgtt tcaaaggcca attctctatg aatttattta tagttgtcat tagtaagaca
    12541 attaatagag tgtttgtaaa gactgctaaa ctaagcattt tagcacctgt gattgtatgt
    12601 cctaaaatgg acactacttc aaaattttgc ttaagattga ccgagaaata attatcaacc
    12661 tggagccaga caacactgga accaattgct cctaaagtat aaacaacaaa cgctttatcc
    12721 actaaaacct gactataatt tttaaaagtt cctaaaactc ccttatcatg cttgaaggtt
    12781 ccctcctgtg gttttgtctc atcaaacttg aagtaaacca cgaaacaaca aatcgctgaa
    12841 ataataagaa gaactattaa taattcaaaa aaatggtgat catagaaaat cccagcaatc
    12901 ccagaaccta gcattacagc gatgttaacc aaccaataac ctatagtgta aacaaagcga
    12961 cgattctgtt cgttcgtcaa atcaatggtc atagcttcat aggcagggaa ataaaattgg
    13021 tgagctattt caatgataag gatacctaca aaggtcaagt gtggcgtaat atgatttgga
    13081 acatttgcag ctatagttat agcccaacca atggttgcca gcagtgaacc tacaatcaca
    13141 acttttttcc tccctatagc atctgacaaa tggcctccat ataaggtcgc tacaaaccct
    13201 gatagttgag ttatgatgat caaaattcct gtaactagat ttccaaaata ttgaacataa
    13261 tacattgcca taaaaggaaa aatcgctgaa ccaactgtaa tggaaataaa acgcaataat
    13321 tctcttaact gtagctgttt tggcaaagca aaaaactctt tcactttttc tcccttcttc
    13381 cctaacaagt aattatcaat attttcctaa atccctgagg cttgtatgat tttcttggat
    13441 tcgtttaaac attttctcca tatcagcttt tgtaaaatta actaaaacag gtcttccatg
    13501 agggcaatta taaggatttt tacattgcgc taactgatct aataaatgac gtgctgagta
    13561 atcgtctagc gtatggtttg ctttaataga tcgcttgcaa gacatcataa tagccaactc
    13621 tgcgcgatat tttttcactg agacttcatt agtgagcaac aacatatcac acatttcata
    13681 aacgccagac tctacctctt cttctttcat ccaaattgga tgttccctta aaatgaaagt
    13741 attattacca tacggttcca agaaaatacc aacatcttgt aataatgaca ttttttcttg
    13801 taattgaaga aaatctgaac ttgaaaattc aaatagaaat ggtactaaca attgttgaag
    13861 actattatca acctcaccta tcttttcacg gtaatactcg tattttacac gctcttgagc
    13921 tgcatgttga tcaataatat acaacccacc attcccttga gcaaataagt aggtcccatg
    13981 catttgacca aaaaattcga gctctggaaa agtcgaactt tcttccgaat ctaatttttc
    14041 aacaagtttg ttaagtgact gtttactact aaggtagtcc tgagaatgaa tgaagttctc
    14101 actatcagct tgatctctac tagcatactt tatattagga gactgcgttg ctccttgatt
    14161 aacagagttt tttacatcat tgtcaactga accgtcactt ttgtcaaggt ttgtaaagtt
    14221 agcaataggt tcagatacag tatctgcatc tataaaaaag tcatttttag tcctatcgta
    14281 atacaaacca ggctgtttta gtgagaagct tgtttgtata ggcttatcta cacttcgggt
    14341 actagtttta gctaaattct ctaaagcatc cggaatcaaa tcatattgtt taagactctc
    14401 agaaattgct gagcttatta aactcattaa ttctcgttcc ttagaaattc tcacctcttg
    14461 cttagtcgga tgaacgttga catctgctag ataaggatct atctgaatat caatgacagc
    14521 aattggaaat cttccaacca tcaactttga accataacca tctagaattg agcggtttaa
    14581 aagaaaattt ttaatgtaac gaccgttaat taatagggta atatagtttc ggttagctcg
    14641 tgtcaattca ggcaaactta cataacccga aatttcaaaa tctaaatctg catttgaaat
    14701 ctcaatcatt ttttttgcag tatttaaacc ataaatacct gcaatcgcct gtctcaaatc
    14761 gccagtccca ctagttttag tcatttcttt accatcattt atcagtgtaa aagcaacttc
    14821 cggatgcgct agactgagtc tattaacaat atcaataata tgagctagtt cagactgtaa
    14881 actcttcatg tatttcaatc tggcaggcgt attaaagaat aaattttcaa ctaaaatttt
    14941 tgttccacga ggacttgaaa caacttcctg tttttcaatg tttccccctt tagcaacaag
    15001 taacgtccct tgtttccctt gctctgtagc tgtttttatc gtcattaaac taatcgaagc
    15061 gattgatggt agggcttcac cacgaaaacc taaggtccga atacgaaaaa ggtcactctg
    15121 gcttttgatt ttggatgtag cgtgacgtcg taaactgaga acagcatcct cactagtcat
    15181 cccttcaccg ttatccgtta tttgaatctt tttaagacca gattcttcaa cctctatggt
    15241 aatttggcta cttcctgcat ctatggcatt ttcaactaat tctttaacga cgctactagg
    15301 tctttcaaca acctcaccag cagcaatctg gttagctaat atatccggta attcaataat
    15361 tttagacaag ttcatccctc ctttttttaa gattttttgt aacttttttt gtgatatctt
    15421 agccattata ccatattcaa aatattaatt tgacaataac acaattttta gttgacatca
    15481 ctcctattac ctgttagact tagggagcgt aataagtttt gaagttgtat ttttgtttaa
    15541 catttttacc ttttttattt tttacagtaa catttttaga aaggtaataa acaaatgaca
    15601 caaggtacag tgaaatggtt taactcagaa aaaggttttg gctttatttc tagcgaaact
    15661 ggaacagacg tcttcgctca tttttcagag attaaagttg atggcttcaa aactcttgaa
    15721 gaaggacaaa aagtaacatt tgatattcaa gatggtcaac gtggaccaca agcaacaaat
    15781 atcaatcttg ttaaataatt aaaaaagagc ctaaatgaat tatttagtct cttttttgtt
    15841 gcccaaaggc tattttataa taatttcttt aagtcaaata tagcattcat agcttgcatt
    15901 ggagttagat tcattatatc tatcgcttct aactcctcaa taaccctacc tgcatttgaa
    15961 ggttcttcaa aaagtgataa ttgtccttgt tgcttctcag aatctagttc ctcatttaag
    16021 tcagcagtaa cagcttcttg gggggctgat acgatcaact gtactgcatc agcttccaac
    16081 tgtgataaaa tatcagttgc cctatccaat aagtcaattg gtaaaccagc tatttttgcg
    16141 acgtgtatcc cataagactt atccgcaggt ccagattcaa ttttatgtaa gaaggtaact
    16201 tctccatctc tctctaaagt agccacgtgt acattgacaa gccttgtcaa ttgttcagat
    16261 aaatctgtca actcatggta atgagttgca aacattgttt ttgccctaac acggtcatga
    16321 atatattcta taatcgattg agctaatgcc ataccatcat aagtggctgt ccctcgacct
    16381 aattcatcaa aaagaatcaa agatttatca ctggctcgtt ttacagcctg attcgcttcc
    16441 atcatttcta ccataaaggt tgattgccca gaaattaagt catcagcagc accaatccta
    16501 gtaaatattg catcaaatac aggcaaatca acttcgtctg ccgatacaaa acctcccatt
    16561 tgtgccataa taactgtcaa agctaactgg cgcatatagg tcgacttacc actcatattt
    16621 ggtcctgtaa tcaactggat atctgtctga ctatcaaaat agatactatt gggaatatat
    16681 tcttgcactc ccatcacttt ttcaacggtt gcatgacgcc cattcttaat cttaatctga
    16741 tgttcatcat taaatttggg acgaacatag tgatgatttt ctgcaactac tgccaaactc
    16801 tgtagaacat caacggttgc aatagtcttt gctagctctt gaagacgttt aatataagat
    16861 tctacttgag cacgaacacg cataaaaata tcatattcta aatttgaaga ttgctcgcga
    16921 gcttcgagca cttcaccttc aattttggct agttctgctg ttccatagcg ttcagaattt
    16981 tttaacgtcg ctttacggaa aaaatgctcc ggtactagtg ataaattgga attggtaaca
    17041 tggaagtaat aaccgtcttt tttattataa tcaattttaa gagtaccgat accacttgct
    17101 gctctttcct tagcttcaat atcagcaatc caacctgtac cttcacgcat cactgttcga
    17161 taattatcca attgcttatc aaatccagac ttgataatgt ttccctcagt gatagtagcc
    17221 tgtgcttctg gggctatcgc cgtattaatt aaactttcta attcaggcat agtgtcaatt
    17281 ttgttgacaa caatgtcaag ctcaggttga ttgaaggact gtaaaatcat tttaatccga
    17341 ggaatttgtg ataaggtctg tccgagttgc aatagatctt tagggttggc ttttccgaaa
    17401 gatactcgac ttgctagacg ttcaatatca tatacaccct ttaaactttc tgtgagatcg
    17461 tttctctcaa aaaaataatc aagaaacact tgaataatat cttgacgttc cttgattcga
    17521 ttcatactta ccaaaggcct gtcaatccaa gttctcagca ttcgagttcc catcgctgtt
    17581 ttagtctcat ctaacaacca gtaaagactt ccgtgcttct tgcttgttct agcattctcc
    17641 agtaaatcta gactattttt cgttgcatat gacatttgta aatagtcctt tatttcataa
    17701 tgaactactt tctgtaaatg actaagttca cgcttttgtg ttctatgcac atattgtaaa
    17761 agtttttccg cagcagatat ttccaaatca gttaactgtt catcaatcaa atgaatatca
    17821 ttaagtcgtt cgtcttcgta tgataaaagc aagttcatct gcttcgtcag taggtgattt
    17881 ttttcgtccg tcaattgata tcctactaca atctcacgag cttttaaatt tagtatttca
    17941 ctacggacgg actcaaaatc tgttaaaagg gttgcctgaa actctccagt tgagacatcc
    18001 atatatgcta gaccaaatgt ttgttgatct tgcgaatcaa tcgctactaa gaaattatta
    18061 gcactatccg gcttcgttga ctccacaacc gttcctgggg taacaacttg cactacctca
    18121 cgctttacca ctcccacagc ttttttagga tcttccatct gctcagcaat ggctactttg
    18181 taacctaatt caactaaaac gtcaatatac tgttgagctg agtgataggg aacacctgcc
    18241 attgggattg gcttttctgc gttcttattt cgactagtca agctaatctc caggatttgt
    18301 gctgctttta ccgcatcatc ataaaataat tcataaaaat cacccattct aaaaagcaaa
    18361 aaagcatctg gataattctc ttttatatcc agatactgtt gcattcccgg tgatatcgtt
    18421 ggcttggcca tttctcactc ctaactaacg gggctggctg ccatgctcag ctcctcaatc
    18481 attatattta taaacctact gatagttctt tttcaaaaac tttggcaata tcttcatttt
    18541 gacaaatcac caacaaggtg tctgctcctg ccattgttcc taaaatctct ggcagccctt
    18601 tctcatcaat aaagtttgct aaaacgctag cctcccctaa attagtattt aaaactaaca
    18661 taaaacttgc tcgcgatact ttcaaaatat aggagcgtaa ggctgggcta aatcgatttg
    18721 aagtagctgt tgataaacta taatatagct taccttctgg gctacgcaat tttaaaagac
    18781 caatctcacg caagtctctt gatagagtcg cttgcgtgac atcgattcct tcatttttta
    18841 aatgtaattt aatttcttct tgtgtgccaa tctgccctga ttggattaaa cgtttaatac
    18901 gtttttggcg ttctatttta ttcatgttat tcaatccttt ttagttagaa ttatgcttac
    18961 caaattatat caaaaaaaat tgtctttttc acttagtagt gatatcgtaa tcattctgta
    19021 aaaaagctat aaagtaggct agttctttac agaatgcatt atga
//
LOCUS       DATRSD010000040        18930 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRSD010000040 DATRSD010000000
VERSION     DATRSD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.040"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            93..1283
                     /locus_tag="VBD85_001129"
     CDS             93..1283
                     /locus_tag="VBD85_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7133689.1"
                     /translation="MNRPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLEWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTK
                     LRQPTDRILREMNSQMSGYVQGQIIVAITVGIIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTVDVVTEERSEEV
                     KNVE"
     gene            1273..2499
                     /locus_tag="VBD85_001130"
     CDS             1273..2499
                     /locus_tag="VBD85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133690.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEVLLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIMGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVSSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2609..4291
                     /gene="alsS"
                     /locus_tag="VBD85_001131"
     CDS             2609..4291
                     /gene="alsS"
                     /locus_tag="VBD85_001131"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO7133691.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLYLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQENTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4305..5024
                     /gene="budA"
                     /locus_tag="VBD85_001132"
     CDS             4305..5024
                     /gene="budA"
                     /locus_tag="VBD85_001132"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO7133692.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHYIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLEANFDLQKLREDIDLSE"
     gene            complement(5078..6733)
                     /locus_tag="VBD85_001133"
     CDS             complement(5078..6733)
                     /locus_tag="VBD85_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO7133693.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDVVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTENTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQGKIDSLKLKK"
     gene            7091..8095
                     /locus_tag="VBD85_001134"
     CDS             7091..8095
                     /locus_tag="VBD85_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7133694.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVPHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            8108..8971
                     /locus_tag="VBD85_001135"
     CDS             8108..8971
                     /locus_tag="VBD85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133695.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLVISALIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8971..9732
                     /locus_tag="VBD85_001136"
     CDS             8971..9732
                     /locus_tag="VBD85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133696.1"
                     /translation="MVMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSGGNIYIMGQDVTNLPAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRILVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            10029..11690
                     /locus_tag="VBD85_001137"
     CDS             10029..11690
                     /locus_tag="VBD85_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7133697.1"
                     /translation="MSDIKIMALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNDQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGFVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11747..12535
                     /locus_tag="VBD85_001138"
     CDS             11747..12535
                     /locus_tag="VBD85_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO7133698.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEISDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNINYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYFNDHGKHEWYYWNQQLEKVLEWLPIDYVKEERLS"
     gene            12625..13335
                     /locus_tag="VBD85_001139"
     CDS             12625..13335
                     /locus_tag="VBD85_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO7133699.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFTNLATDSVHHSENTRWDKNQATIY
                     IATRDSELVSAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETEKN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13573..14244
                     /gene="rpiA"
                     /locus_tag="VBD85_001140"
     CDS             13573..14244
                     /gene="rpiA"
                     /locus_tag="VBD85_001140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO7133700.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVVGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNSMVNKVIVAGKNGL
                     DILEK"
     gene            14301..15512
                     /locus_tag="VBD85_001141"
     CDS             14301..15512
                     /locus_tag="VBD85_001141"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7133701.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDECLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            15563..15970
                     /gene="arsC"
                     /locus_tag="VBD85_001142"
     CDS             15563..15970
                     /gene="arsC"
                     /locus_tag="VBD85_001142"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO7133702.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSRMEL
                     VELLLEMKLSARELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPILINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            16009..16818
                     /locus_tag="VBD85_001143"
     CDS             16009..16818
                     /locus_tag="VBD85_001143"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7133703.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            16820..18076
                     /locus_tag="VBD85_001144"
     CDS             16820..18076
                     /locus_tag="VBD85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO7133704.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLASFLASLSWYYLQIKPKQITSIKQQVVFKDSSLKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIISGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLASTFGAASIAQLVISTAPLYRIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IVYPQILGNGNALVQEVFKGTTVSLITILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHISIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            18060..18770
                     /gene="deoD"
                     /locus_tag="VBD85_001145"
     CDS             18060..18770
                     /gene="deoD"
                     /locus_tag="VBD85_001145"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7133705.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            18779..>18930
                     /locus_tag="VBD85_001146"
     CDS             18779..>18930
                     /locus_tag="VBD85_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7133706.1"
                     /translation="MTDFSTALKVLVDQYSYHNAFLLLQKHGPLNSDLLFLLEMMKER
                     RELNID"
BASE COUNT         5997 a         2970 c         3877 g         6086 t
ORIGIN      
        1 actttttaca ctcttgtttt gagtttaatt taatttaacg tgttataata aatgagaatt
       61 aaaattagaa ttcatcttga aaggatatgt aaatgaatcg accatcagaa aaagaattta
      121 aaaatagttt gttttttaaa tggatcttaa ataatcaagc agttattgct ctcatgatta
      181 cctttttggt atttttaacg atttttattt ttaccaaaat ctcttttatg tttaaacctg
      241 tgtttgattt tcttgctgtg ctgatattgc cgcttgtaat ttctggattg ctttattacc
      301 tattaaaacc tatggttaca tttttagaga agcggggaat taagcgtgta acagcgatat
      361 tatcagtttt tactattata atccttctgt taatttgggc aatgtctagt tttattccca
      421 tgatgagtaa tcaattacgc cattttatgg aagatctccc ttcatatgtg aataaagtgc
      481 aaatggaaac aagttcgttt atagatcaca acccttggtt aaaatcttat aaaggggaaa
      541 tatcgagcat gttatctaat atcagtagcc aagcggtctc ttatgctgaa aaattttcaa
      601 agaatgtttt agagtgggca ggaaatttag ctagtacagt tgcacgtgtg acagtagcaa
      661 caatcatggc tccctttatt ttgttttatc ttttaagaga tagtcgcaac atgaagaatg
      721 gtttcttaat ggttttacca accaaactac gccaaccaac tgatcgtatt ttgcgagaaa
      781 tgaatagtca aatgtcagga tatgtgcaag gacaaatcat tgttgctatt actgttggta
      841 ttattttttc aataatgtat agtattatag gccttagata tggcgtgaca ttagggatta
      901 ttgccggtgt gttaaatatg gttccctatt tgggaagttt tgtcgcccaa attccagtgt
      961 ttatcttagc gcttgtcgca ggacctgtta tggttgttaa agttgcgatt gtttttgtta
     1021 ttgagcaaac tctagaggga cgctttgtct cacccttggt tttaggtaat aaacttagca
     1081 ttcatccaat tacaattatg tttattttat taacctctgg agcgatgttt ggtgtttggg
     1141 gagtattcct cagtattccg atttatgcat ctatcaaagt tgttgttaaa gaattgtttg
     1201 attggtacaa agctgtcagt gggctatata cagtagatgt tgttactgaa gaaagaagtg
     1261 aagaagttaa aaatgttgaa tagtgaaaaa atgattgttt ctatccaaaa tcaggattta
     1321 gaacatgcta acaagtattt cgaaaaagcg ttgaaaaatg atcctgagga agtcttattg
     1381 gaactaggag cttatttgga gagtattggt tttttacccc aagcaaaacg tctctatgat
     1441 caaatccgac caaattatcc agaagttgct atcaatttag ctcaaattgt tgccgaagat
     1501 ggagatattg aacaagcatt cttatactta gattatatct cagaagacag tcaagagtat
     1561 gtttcagccc tccttgttat ggctgactta tatgatatgg aaggcttgac tgatgttgcg
     1621 cgtgaaaagc ttttgctagc cagcaaactt agcgatgacc cattggttac atttggttta
     1681 gctgaaatga accttagttt ggaacattat caagaagcga tagaaggata tgcttcactt
     1741 gacaatcgag aaatcttaga aacgactggg gtttcaactt atcagcgtat tggtaaatcc
     1801 tacgctatta tggggaaatt tgatgcagct atcgaatttt tagaaaaagc cgttgacatt
     1861 gagtatgatg atttaacagt ttttgagtta gcaacaatcc tttatgatca agaagaatat
     1921 caaaaagcta acctttattt taaacagttg gatactatta atccagactt tgatggctat
     1981 gaatatatct atggtttatc tcttcgtgag gagcataaat ctgaagaagc tttacgtcta
     2041 gttcagcaag gtcttcgtaa aaatagtttt gatggtcaac ttctcttact agcctcacaa
     2101 ttatcgtatg aattacatga tgtttcttcg tcggagtctt atttgaaaca agctgaaaaa
     2161 gtttctgaaa accaagatga aattgttatg agattgtcaa atttgtatct tgaagaagag
     2221 cggtttgagg aagtcttgga attagacaat gatgatttag aaaatatcct tgcaaaatgg
     2281 aatatcgcta aagcacacaa ggctttagaa atggatgata gtgtagacta ttatcaatct
     2341 ttgtataatg atttaaaaga taatccagaa tttttacaag attatgctta cattttgaga
     2401 gaatttggtt accttgataa ggcacaagaa gtaggaaagg cttatttaaa attagttcca
     2461 gatgatatag agatgtcaga atgggtgaat aacatttaaa taggaaaaca cttacagaaa
     2521 aaaataaaaa attcacaagt aaattttcac aatctgatgt aaaaactgtg atataattga
     2581 accattcttg tgaaaagagg gagataaaat gacagaatca cataatcaat atggggctga
     2641 cttaattgtc gatagcctca ttaatcatga tgtcaaatat gtttttggta tcccaggagc
     2701 taagattgac cgcgtatttg acactctaga agataaaggt ccagagttaa ttgttgctcg
     2761 tcatgagcag aacgcgactt ttatggctca ggctgtcggg cgtattacgg gagaaccagg
     2821 agttgttatt gcaactagtg gacctgggat ctcgaacttg gcaacagggt tagttactgc
     2881 gacagatgaa ggtgatgctg ttcttgcaat tggtggacag gttaagcgag gagatttatt
     2941 gaaacgtgct catcaatcaa tgaataacgt tgctatgcta gaacctatta caaaatattc
     3001 tgctgaggtt catgacccta atactttgtc agaaactgtt gcaaacgcct atcgtcttgc
     3061 taaatctggt aaaccaggag caagttttat ctctattcct caagacgtta cagacagtcc
     3121 agtctcagta aaagcaatca aacctcttag tgcacctaaa ttgggttcgg catctgtttt
     3181 agatattaat tatttagcgc aagctattaa caatgctgtt ttaccggtac tgttattagg
     3241 aaatggcgca tcttcagagg gagtaactgc tgccgtacgt cgacttcttg atgctgttaa
     3301 actaccagta gtagaaactt tccaaggtgc gggtattgtg tcacgtgagc tcgaggatga
     3361 aacattcttt ggtcgtgtag gcctcttccg taatcaacct ggagatatgc ttttgaagcg
     3421 tgcagaccta gttattgcta tcgggtatga ccctattgaa tatgaagcgc gtaactggaa
     3481 tgctgaaatt tctgctcgta ttattgttat tgacgttgaa caagcagaaa ttgatacgta
     3541 tttccaaccc gagcgagaac tgattggaga catggcacat acactttatt tacttttacc
     3601 agctattaaa ggatatgaat tgcctgaagg ttcaaaagag tatttgaaag ggcttcgtaa
     3661 taacattgaa aatgttagcg atgttaaatt tgatcgtgac tcagctcatg gacttgttca
     3721 tcctcttgat ctcattgatg tactccaaga aaataccacc gatgatatga ctgttacagt
     3781 tgatgtgggg agtcattata tttggatggc tcgctacttt aaatcatatg aagcgcgtca
     3841 cttgctcttc tcaaacggaa tgcaaacatt aggagttgct ctaccttggg caatttctgc
     3901 ggctcttttg cgtcccaaca caaaagttat ttcagtttct ggtgatggtg gcttcttatt
     3961 ctcagctcaa gagttagaaa ctgcagttcg tcttcatcta ccaattgttc acattatttg
     4021 gaacgatgga aaatataaca tggttgaatt ccaggaagaa atgaagtatg ggcgttcttc
     4081 aggtgttgac tttggaccag ttgattttgt aaaatacgct gaaagtttcg gtgctaaagg
     4141 ttaccgtgta gacagcaaag acagttttga agaaacactc aaacaagctc taatcgatgc
     4201 tgaaaatgga ccagtgttga ttgatgtgcc tatcgactat aaagataacg tgacactagg
     4261 tgaaacaatc ttacctgacg agttctacta agacgaggtc taatatgtca gaaacagtta
     4321 aattatttca atacagcacc ttgagttcac tgatggcagg attgtacaaa ggcagtctaa
     4381 ctatcggaga attattaact cacggtgact taggaatcgg tactgttcat atgattgatg
     4441 gtgaattgat tgtattggat gggaaagctt atcaagctat tggtacagat ggtaaagcag
     4501 agattatcca attatcagat gatgtgacag taccctatgc agcggtttta ccacactata
     4561 tccaaaaaca atttgatatc aatgctgaga cagataataa ggatttggaa gagatgattc
     4621 taaaaaactt tgaaggtcaa aatctcttta agtctttaaa aatcaaagga acgttttcaa
     4681 gaatgcacgt tcgtatgatt ccaaaatctc cacaacataa acgttttgca gatattgcat
     4741 ctaatcagcc agaatttacc agagagaatg tatctggtac gttggttggg atttggacgc
     4801 cggaattatt tcatggtgtg ggggtaaaag ggttccatgt ccactttatt tcagacgatt
     4861 taactttcgg tgggcatgtt atggattact cgctcacaca aggaaaagtt gaaattggga
     4921 aggttgacca acttgaccaa tgtttcccaa ctcaagatca agaatttttg gaagctaact
     4981 ttgacctaca aaagcttaga gaagatattg atttaagtga ataagaatac ctataaataa
     5041 ttaataacac taaaaataga cagggatatg tcccagtcta tttttttagt ttaaggctat
     5101 ctatttttcc ttgtgtggga gtgactcgta aggttttttg accagtgtaa gttacaaaac
     5161 ctggcttagt accacttggt ttattgagtt tttttgcttc aatcatgtct acttgaacaa
     5221 gattggagag acgagcttta gagtaataag cagctaactc ggcggcatct gtcttaacct
     5281 cgtcacttgg attgaggtta tcccgaatta acacatgact tccggggata tccttggcat
     5341 gaaaccacaa ctccccttta cgagccattt taaatgttaa ctcgtcattt tggagattat
     5401 tacgtcccac cataataata gtcttgccat cggatgctaa atattgttca ggttttttgc
     5461 gcttatgttg tttatcatgt gcgcgtcgct taataaatcc agtttcaaca agttcttcac
     5521 gaatatcatt gatatcttcc atagaagcat gatttagcga tgtttcaaca gattcaaggt
     5581 aggtgattgt attttccgta tctgaaataa ttccttttaa atgttttaca gcttctttta
     5641 atttttgata ttttttaaag tagcgttggg cattttggtt gggtgttaaa gctcgatcaa
     5701 gtgaaatctc aatggtttgg ttggtgtaat aattatcaag cacaacgaca tcttgattat
     5761 ttggtaccat agagagatag gtagttagaa gctctccttt ttggcgaaac tcctctgcat
     5821 tttcagtagc tagaagttcg tcttgttgct tagctagttt tttgatattt ttctctaact
     5881 cactttgaac acggtggatg aggtcactag cttgctgtgc tatgcgatct ttttcagcct
     5941 tctcttggta gtaataatct aacaattgtc caagagactg aaatgtttcg tgactgcttg
     6001 agaattggac agcggaaaag gatttttctg ttaggctggg ataatattct ctcgaaaaga
     6061 attgacgaaa atcgttaagt ttattgtctt tcaaacagtg agataattct agcgcagtat
     6121 cgcgtcctaa accttggaag agctgttgga ggtttttagg gcttagatca ttggtttgaa
     6181 gcagctcaaa tagagtttga tcagaaatat caaaaggatt gattgctttt gttttgggtg
     6241 gggcaatgta agtcgaacca ggaaggatag tacgatagga gttttgtgag aaaccaacat
     6301 gcttaattga ttcaataatt ttatgttcat ttttatcaat caagataatg ttactgtgtt
     6361 ttcccatgat ttcaacaaca agtgttgctt taatgtgatc accgatttcg tttttattag
     6421 acacgatgat ttctaatata cggtcatttt caatttgttg aatggtttca atcactgcac
     6481 cttgcaaata ctttctcatg atcattgtaa aagtatttgg attttgagga ttttggaaat
     6541 ttgcttcggt agtttggata cgtccaaata cagggtgagc agataggagt agtttatagt
     6601 tgcgacgatt attgcgtatt gttaatacca attcatggtc aaaaggttgg ttaacttttt
     6661 ggatacgtcc tttttcaatc tgttcttgta attcatttgt gaggtgatgt aaaaaaaatc
     6721 catcaaaaga catggcgtcc cccttattct aaaaattata gtttatttat tataccatag
     6781 gctgagctaa aaatattttt tataacgcaa aaggcttgca attttcagaa tatagtatat
     6841 aattatagaa aaattcatga gaaaggatga ctagtaaatg actaaaacat tatttaccaa
     6901 ttggcaaaac tggcgtagat aaagttgtgt ttatgggtgc atagatacga ctttttggag
     6961 tacttcaaaa aagtatctat gcgctggtct agtctaacaa tagagagcgc aaggtaagtt
     7021 atttatcagg cgctttttct tttgcgctaa actccagaaa gtcagagtat tattaaagtg
     7081 aggagaaaat atgaaaaata aaggactgat agctacgcta attctattga ccatattagt
     7141 ggttggggaa ttattttata acaaatcaga aaaacatctt aatttatcag aaaagcaagt
     7201 tgttaaaatt ggtattttac agtatgtacc ccatgatgct ttggatgcta ttgaaaaggg
     7261 agttgaagat ggtctagctc aagaaggata taaagggaaa aaagttaaat taaccgtttt
     7321 aaacgccgaa gctgatcaaa gtaaaattca agcgatgagt aaacaattag ttaatcatca
     7381 caatgatatt ttgattggaa ttgcgacacc atcagcacaa ggtttagcag cttcgactaa
     7441 ggatactcct ataatcatgg gagctgtttc tgatccttta ggggccaagt tagtaacgaa
     7501 tatgaagaaa ccaacaacta atgttacagg attatcaaac gttgttccga ccaagcaaac
     7561 tgtgcaatta atcaaggata taactcccaa tgttaaacgt ataggaattc tttatgctag
     7621 tagcgaagat aattctgtgt cacaagtaac tgagtttaca aaatatgctc aaaaagcagg
     7681 actagaagtt cttaaatatt ctgtaccatc tacaaatgaa attaagacaa gtatgtcagt
     7741 catgaccaaa aaagtagacg ctgtctttgt tccacaagat aatactattg cgagtgcttt
     7801 tagaacagtt atcgtagcag caaaccaagc taatatacct gtgtattcca gtgttgatac
     7861 tatggttgaa caaggaagta tagcatcagt agcgcagagt caatatggct tagggttaga
     7921 aactgcaaaa caggccatta aagtcctaag agggaagccg gttaaagatg taccagtcaa
     7981 agttattgat acagggaagc caagcctcaa tttgaaagca gccaaacact taggaattaa
     8041 aattcctaag aagattatga aacaggcaga aattaccgtt aaggttgacg attagaaaga
     8101 gggttttatg attatttcat cagtttcaca aggtttatta tggggaatat taggattagg
     8161 gatatactta acctttagaa tcttaaaatt tccagacatg acaacagagg gctccttccc
     8221 tctaggggga gcggtttgtg ttaccttaat gaatcagggg gttaatccta ttttggcgac
     8281 gattctaggc atgctatcag ggatgttagc gggatttgtt actggtttac tttatactaa
     8341 aggaaagata ccgacgattt tagcaggaat tttagtaatg acctcatgcc actccattat
     8401 gctaatggtt atgaaaagag caaatctagg tttaaatgag attcaaacat tgaaagattt
     8461 tcttccgttt tcaaatgatt taaatcttct cgtattaggt ttgatagcta ttctcttggt
     8521 gatttctgcc ttaatttatt ttctctatac acgcttgggg caagcttata ttgcaacagg
     8581 agacaaccct gatatggcaa agagcttcgg cattgatacc gacaaaatgg aaatgcttgg
     8641 cttaattgta tcaaatggct taattgcttt atcaggagct ttagtaagtc aacaagatgg
     8701 atatgctgat gtatcaaaag gtataggtgt tattgttatt gggttagcta gtattattat
     8761 tggagaggta ctttactcta ctggtttaac tctctttgaa cgtttaattg ctatagtggt
     8821 gggttctatt ctttatcaat ttttaataac ggcagtaatc gccttaggat ttaatacgaa
     8881 ttacctaaaa ttatttagtg caatagtgct tggaatttgt ttaatggttc ctgtcctaaa
     8941 aacgaagatt ttgaaaggag tacgcttata atggtgatga aaatcataga gttaaaagaa
     9001 gcaaccgtac aagtcagtaa tggtttagca gaaatgaaaa cgatattaga ccatgttaat
     9061 ttgagtattt atgaacatga cttcattaca atattgggtg gaaatggagc tggaaaatca
     9121 acgcttttta atgtaattgc aggtaccttg atgttaagcg gtggaaatat ttacatcatg
     9181 ggacaagatg ttactaattt accagcagaa aaacgagcta aatatttatc acgggtattt
     9241 caagatccga aaatgggaac agcacctagg atgacagttg ctgaaaattt attagttgct
     9301 aaatttcgag gtgaaaagag accgttagtt cctagaaaga ttactaacta taccgaagaa
     9361 tttcaaaaat tgattgctag aactggtaat ggacttgatc gccatttaga gacacctaca
     9421 ggtttattat caggtggaca aagacaagca cttagtttat taatggcaac tttgaaaaaa
     9481 ccaaatctgt tattattgga cgaacacaca gcagcgcttg atcctagaac aagtgtttcg
     9541 cttatgggct tgactgatga gtttatcaaa caagattcgt tgactgcctt aatgattaca
     9601 catcatatgg aagatgctct caaatatgga aatcgtatat tagtaatgaa agatggcaag
     9661 attgtcagag atttaaatca ggcacaaaaa aacaagatgg ctatagctga ctattatcaa
     9721 ttatttgatt aaaaagaagt gaaatgggag tgtttcactt ctttttctat acactttgca
     9781 agaaattatt ttcaaaatat gatatactaa taatgctgta aatcgacttg tagagacaag
     9841 gaccgtaagt acaaatggaa tgaaagtaag gacacactct ttaattcata ttcctattat
     9901 tgattaataa ggtttttctt caaacccaag aaaagttacc gataaatcaa ttactaatga
     9961 taataattgg acaatttctt gtctgcttta taaaaaagaa ttaacaaaat ttaaggagaa
    10021 aactcattat gagcgacata aaaatcatgg ctcttggagg tgttcgtgaa aacggaaaaa
    10081 acctatatgt agtagaagtc aatgattcta tctttgtttt ggatgctgga ctcaaatatc
    10141 ctgaaaatga acaactgggt gttgatgttg ttattccaaa tcttgattac ttaattgaaa
    10201 ataaaaaacg tgttcagggg attttcttaa cccatggaca tgcggatgct attggtgcat
    10261 tgccttatat tatcgccgaa gtaaaagcac ctgtgtttgg ttcacctctc acaattgaat
    10321 tagctaagtt attcgttaaa aatagtacag cagtcaagaa attcaataat ttccatgtga
    10381 ttgatagtga aacggaaatt gaatttcaag atgcggtgat ttcgttcttt aaaacgactc
    10441 attcaattcc agagtcaatg ggaatcgtta ttgggacaaa agagggaaat atcgtttaca
    10501 caggtgactt taaatttgat caagctgcaa ggaaatatta ccaaacagat ctggcacgtt
    10561 tagctgaaat tggtcgtgat ggtgttttag cacttttatc agattctgca aatgctacaa
    10621 gcaatgacca agttgctagc gaatatgagg tcggtgatga aattaagagc gttattgaag
    10681 acgcagaagg tagagtcatc gtagcagcag ttgcaagtaa tctcattcgt attcaacaag
    10741 tctttgatgc agcggcagaa aacggtcgtc gagtagtttt gactggtttt gatattgaaa
    10801 atattgttcg tacggccatt cgtatgaaac gcattcatat tgctgatgaa aatatgatta
    10861 tcaaacctaa agatatgaca cgttacgaag ataacgaatt attaatcctt gaaactggac
    10921 gtatgggaga gccgataaat ggtctccaaa aaatggcaat cggacgtcat cgttatgttc
    10981 aaattaaaga tggtgattta gtatttatcg taacaacacc aagtattgca aaagaggcag
    11041 ttgtagcgcg tgttgaaaat ctgatttata aagctggtgg ctcagttaaa ttgataacac
    11101 agaatttgcg cgtatcaggt catgcaaatg gtcgcgaatt acaattatta atgaatctat
    11161 tgaaacctaa ataccttttc ccaattcaag gagagtatag ggatttgtca gcacatgcgg
    11221 gcttagctca agaggtagga atgtcagcag atgatattta tattgtgaag cgtggtgaca
    11281 tcatggttct tgaaaaagat ggcttcttcc atagtggatt tgttcctgct ggagatgtta
    11341 tgattgatgg aaatgctatt ggtgacgttg gtaatattgt acttcgtgac cgcaaggtat
    11401 tatctgaaga tggaattttt attgttgtta taactgtcag caaaaaagaa aagaaaatca
    11461 tttctaaagc tcgtgttaat acgcgtggtt ttgtgtatgt gaaaaaaagc agagatattt
    11521 tgcgtgagag cgcagaatta gttaatacaa cggtagaaga ttacttaagc aaagatacat
    11581 ttgactgggg tgaacttaaa gggaaagtgc gtgatgaggt cagcaagttc ttatttgacc
    11641 aaacgaaacg tcgcccagca atccttcctg tggttatgga agttcgttga tagtgttaag
    11701 aggttaccaa attttggtag cctttcttgc tagaatggag gtttttatgg cattttttaa
    11761 tattgaatat cactctaagg tactaggaac ggaacgtcaa gtcaatgtta tttacccaga
    11821 tgcttttgag atatctgatg ataaaattga tgattgtgac attcctgttt tgtatcttct
    11881 ccatggaatg gggggaaatg agaattcttg gcaaaaacgc acaaatatag agcgtctttt
    11941 acgtcatact aatcttatcg tcgtgatgcc atcaacagac ttagcatggt ataccaacac
    12001 aaagtatggg ttggattatt ttgatgcaat agctatcgag ttaccaaaag ttttgaaacg
    12061 tttctttcct aatatgtctg ataagagaga gaaaaatttc attgctggat tatctatggg
    12121 gggatatgga gcatataaaa tagctctact gactaatcgt ttttctcatg ctgcctcttt
    12181 gtcaggtgca ctttcttttg actttgattt attattcaat aatggcaacg ataacattaa
    12241 ctattggtcg ggaatttttg gtgatttgaa taacactgat aacatagagc gtcattcact
    12301 aaggcgttac gttgagtctt ttgatatgaa aactaaattt tatgcatggt gtggttatga
    12361 agattttctt tttgaagcta atgaagtcgc aattgatgaa cttcgtcaat taggtttaac
    12421 tattgactat ttcaatgatc atggtaaaca tgaatggtat tactggaatc agcaattaga
    12481 aaaagtctta gaatggctcc ctattgatta tgtcaaagaa gagcgccttt cgtagtatta
    12541 gctaaatcgt tgaaatccaa gtaaagtctt gttatactaa atcattaagt tttgggaaat
    12601 tagacacaga aggtgagata aaaaatgaga acactattta gaatgatatt tgctattcca
    12661 aagtttatct ttagattgat ttggaatatc atttggggaa tattcaagac agttcttgtt
    12721 attgcgatta ttttatttgg cttgtattac tatgcgaatc acagtcaatc agaatttgct
    12781 aatcaactta gtgacattat tcagacagga aaaacatttt taaattttgc tgacactaat
    12841 cagctcaaaa attcctttac taatctagct acggatagtg ttcatcattc agaaaatacc
    12901 agatgggata aaaatcaggc aactatctat atagcaacta gagattctga gttggtctct
    12961 gcttacaagc aagcaatctc caattggaat gcaacaggtg cttttgtgtt acaaacaaca
    13021 gacaatccta atgctgatat tattgctaag gattactcag atgctaaaac acaagctgcg
    13081 ggtgtagctg aaaccgagaa aaatgcttta actaatcgta tttcaaaggt aacagttaaa
    13141 ttaaacacat tttaccttaa gaataaacag tttggttatg accatactag gattgttaat
    13201 acagccgaac atgaattagg acacgcttta ggtctaggac ataatgatca acaacattcc
    13261 gttatgcagt caaaaggttc gcattatggc attcaagaag ttgatattca aacgctaaaa
    13321 aacttatatg cataaaaaga tgagctaaga aactcatctt ttttgtttga acacttattt
    13381 taggtgatat aagtgcgtta gcaatagatt attaaaaata aagaatttga ctatcgctac
    13441 taactaaaat gcataaagaa caaagaaaat gttttcaaaa atcacttaaa aaatggtata
    13501 atgtgtcttg agatagtgtt ttgaatagac ttatcaaaaa tattagataa cattaactta
    13561 aaaggggaaa atatggacga actaaaaaaa cttgctggag tgaccgctgc aaaatacgtt
    13621 aaaaacggta tgattgtagg tttagggaca ggttctacag catatttttt tgttgaagaa
    13681 attggacgcc gtgtcaaaga agagggattg caagtcgtag gcgttacaac gtcaaaccgt
    13741 acgactgagc aggcaagagg attaggtatt ccgttgaaat cagcagatga tattgatgtc
    13801 attgatgtca ctgtggatgg agcagatgaa gtagaccctg attttaatgg tatcaagggt
    13861 ggcggtggtg cgctgctcat ggaaaaaata gtagctactc caactaaaga atatatctgg
    13921 gttgtcgatg agagcaaact tgttgaaacg ttaggtgcat ttaaattgcc tgttgaggtt
    13981 gttagatatg gttctgagcg tctttttcgg gtttttaaat caaaaggtta ttgtccttct
    14041 ttccgtgaaa cagaaggtga ccgttttatc actgatatgg ggaactacat tatcgaccta
    14101 gatttgaaaa aaatagagga tccaaagcag ttagcgaatg aacttgacca tacagttggt
    14161 gttgttgaac atggtttatt taatagtatg gttaataaag ttatagtagc aggcaaaaac
    14221 ggtcttgaca tacttgaaaa ataattattt aaataatcgg ttttaggagc taaccgtaac
    14281 ctccttagaa agaagatata atgtcacaat ttgatcgcat tcacttagtt gtcttagatt
    14341 cagtgggtat tggagcggca ccagatgcca atgattttgt caatgcagga gttcctgatg
    14401 gtgcttcaga cacgcttggc cacatttcaa aaacagttgg acttgcggtg ccaaacatgg
    14461 ctaaaattgg acttggtaat attcctcgtc cccaagcgct taaaaccgtt cctgcagaag
    14521 aaaatccaag tggctacgcg acgaagttac aagaagtatc acttggtaaa gatacaatga
    14581 cagggcattg ggagattatg ggcttaaata ttacagagcc atttgatact ttctggaatg
    14641 gttttccaga agatattatc acaaagattg aggatttttc aggacgtaaa gttattcgtg
    14701 aagcgaataa accttattca ggaacagctg ttattgatga cttcggacca cgccaaatgg
    14761 aaactggtga gctcatcatc tatacctcag ctgatccagt cctacaaatt gcagctcatg
    14821 aggatatcat tcctctagaa gaactctacc gtatttgtga atatgctcgt agtatcacaa
    14881 tggagcgtcc agctctcctt ggtcgtatta tcgctcgtcc atatgtaggt gaaccaggca
    14941 actttacgcg tacagccaac cgtcacgact atgcagtgtc accatttgaa gataccgtat
    15001 taaataaact agatcaagct ggcatcgata cttatgctgt tggtaaaatc aatgatattt
    15061 tcaatggttc aggcattaat catgatatgg gacataacaa atcaaatagt catggtattg
    15121 atactttgat aaaaacaatg gggttatcag agtttgaaaa aggcttctcc tttactaatt
    15181 tggttgactt tgatgctctc tatggtcacc gtcgtgatcc tcatggttat cgtgattgtc
    15241 ttcatgagtt tgatgagtgt cttccagaga tcatttcagc aatgcgtgac aaagacttac
    15301 ttttgatcac tgccgatcat ggtaatgacc caacttatgc aggcacagac catacaagag
    15361 aatacattcc tctcttagcc tatagcccat ccttcacagg aaacggactt atcccagtag
    15421 ggcatttcgc agatatttca gcaacagttg cggataactt tggtgttgat actgcgatga
    15481 ttggagagag cttcttgcag gacttggtat aaatttttat ttccttaaga cacttgtagc
    15541 attaaaaatt gagaggaaga tgatggaaaa agttaggatt taccataacc caaattgtgg
    15601 gacatcgagg aacgtacttg ccattattcg tcattgtggt attgaaccag aaattattta
    15661 ttatctgaaa acaccaccta gcaggatgga actggttgaa ctgttgttag aaatgaaact
    15721 ttctgcgagg gaacttctaa gaacagatgt tcctgcttat gagaaattca atttggaatc
    15781 ttctagcgtt acagatgaag aaatgattga tgcgatgata caagatccta ttcttatcaa
    15841 tcgtcccata gttgttacga gtaaaggtgc aaaactctgc cgaccctgtg aagaaatttt
    15901 aaccatttta cctgtaaaga tggaaaaaga ttttgttaaa gaagatggac agattattca
    15961 aagtttatag catattgtat agtgaataat ggtttaggag gtaagtaaat gacattatta
    16021 gaaaaaatta atgagactag agactttttg caagcaaaag gcgtcacagc accagaattt
    16081 ggccttattt taggctctgg tttaggagaa ttggctgaag aaatcgaaaa tcccattgtt
    16141 gtggattatg cagacatccc aaattgggga cagtcaacag tagttggtca tgctggaaaa
    16201 ttagtgtatg gggatttatc aggccgtaag gtattagcgc ttcaaggtcg ttttcacttt
    16261 tatgaaggaa atacaatgga agtcgttact ttcccagtac gtatcatgag agcattggct
    16321 tgccacagtg tgcttgtgac taatgcagcg ggtgggattg gatacggacc aggaacttta
    16381 atgctgatca aagaccacat caatatgatt gggactaacc ctctcatagg tgagaacctt
    16441 gaagaatttg gaccacgttt cccagacatg tcggatgctt atacagcaac atatcgacaa
    16501 aaagctcacc aaattgctga aaaacagaat atcaaactcg aagaaggtgt gtacttgggt
    16561 gtatcaggac ccacttatga aacacctgca gaaattcgtg cattccaaac aatgggcgca
    16621 caggcggtag gtatgtccac ggttccagag gtgatcgttg cagctcactc agggcttaaa
    16681 gtgttaggaa tttcagcaat tactaacttt gccgctggct tccaatctga gctcaatcat
    16741 gaggaggtcg ttgaagttac tcagcgtatt aaagaagatt tcaagggatt agttaaatca
    16801 ttagttgctg aactttaaaa tggtttctcg attatactat gctgtcaagt ttatgatagc
    16861 cgttctattt atgacagtta tggcaggagt tggagctatc ttaatgcact atgtattaat
    16921 gtttacagag tggttagcat ttggtgatag tagagaaaat actctgtctt tgttaaatag
    16981 tgtcacacca attaaaagag tgttatcact tactttagcc tcctttttag catcccttag
    17041 ttggtattac ttacaaatca aaccaaagca aataactagt attaaacaac aggttgtttt
    17101 taaggattct tctctaaaaa aatcacctta ctggctacat ataggtcacg catttttgca
    17161 attgatttat gtaggagctg gtggtcctat tggaaaagaa ggagcgccac gagagtttgg
    17221 agccataaat gctgggaaaa tatcggattt attagcgctt aaagtgctgg ataaacgttt
    17281 attaatcatt agtggtgcag ctgcaggttt gtcagcagtt tatcaagtac ccttagctag
    17341 tgtattcttt gcttttgaaa ctttagcttt aggaatctca ctgaaaaata ttgtgacatt
    17401 gttagcctct acttttggtg cagctagtat tgctcaatta gttatttcga cagcccctct
    17461 ttatcgtatt tcaaagatga gtctgaattc acagtcatta gcttttatgt tcttgatcgt
    17521 tttatgtgta actccgattg cgattagctt tagatattta aatcaaaaag tgacagagag
    17581 acgtattaaa aatataaaaa tattgttaag tttgccagta gtgtcattga tagtatctgt
    17641 tttatcaata gtctatcccc agattttagg aaatggtaat gccctagttc aagaggtctt
    17701 taaagggacc acagttagct taatcactat tttagttgtt ctcaagatga tagcaacatt
    17761 atccactctt tacgctggtg cttatggcgg tatactgaca ccatcttttt ccattggtgc
    17821 ttgtttaggt tttttgttag ctagtatttc tattccttta cttccccaca tatcgatagt
    17881 aaccagtatg ttggttggtg cagctatttt tttagctatt acaatgagag cgccactgac
    17941 agctgtcggt ttagtcatat cttttactgg tcaatccgtt ataactattg ttcccttaac
    18001 tatagcagta ctttttgcta ctgcctatga ttattttata agaaaaatga ggagtttata
    18061 tgtcaatcca tattgaagca aaacaaggtg aaattgcaga taaaatttta cttccaggag
    18121 atccactccg tgcgaaattt attgccgaga attttttaga agatgcggtg tgttttaata
    18181 cagttcgcaa tatgtttggt tatacaggta cctataaagg acatcgtgtt tctgttatgg
    18241 gaactggtat gggtatgcct tcaatttcaa tttatgctcg cgaactaatt gttgattatg
    18301 gggttaaaac attgattcgc gtaggaacag caggtgctat taatcctgat atccatgttc
    18361 gtgaacttgt tttagcacaa gcagcagcaa caaattctaa tattatccgt aatgattggc
    18421 ctgaatttga ttttccgcaa attgctgatt ttaaactttt ggataaagct tatcacatcg
    18481 caaaggaaat ggatattaca actcatgtag gcagtgtatt atcttcagac gtgttttatt
    18541 caaaccaacc agatcgtaat atggccttgg gtaaactagg tgttcatgct attgagatgg
    18601 aagcagcagc tctctactat cttgcagctc aacacaatgt taatgctctt gctatgatga
    18661 cgatttcaga taacttgaat aatccagaag aagatacaag tgcagaagag cgtcaaacaa
    18721 catttactga tatgatgaaa gttgggctcg aaacacttat ttctgaatga ggatagaaat
    18781 gacagatttt tcaactgctc ttaaagtatt agtggatcag tattcttatc ataatgcttt
    18841 cttattatta caaaaacatg gtcctttaaa ctcagattta ttatttttat tggaaatgat
    18901 gaaagagcgt cgagagttaa acattgattt
//
LOCUS       DATRSD010000041        18249 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRSD010000041 DATRSD010000000
VERSION     DATRSD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.041"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..71
                     /locus_tag="VBD85_001147"
     CDS             <1..71
                     /locus_tag="VBD85_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEO7133707.1"
                     /translation="KKMVWGHITREKQMKDFETSVT"
     gene            311..811
                     /locus_tag="VBD85_001148"
     CDS             311..811
                     /locus_tag="VBD85_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133708.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAAFGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            1009..1257
                     /locus_tag="VBD85_001149"
     CDS             1009..1257
                     /locus_tag="VBD85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133709.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            1347..1583
                     /locus_tag="VBD85_001150"
     CDS             1347..1583
                     /locus_tag="VBD85_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO7133710.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1576..2274
                     /locus_tag="VBD85_001151"
     CDS             1576..2274
                     /locus_tag="VBD85_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO7133711.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            2271..2516
                     /locus_tag="VBD85_001152"
                     /pseudo
     CDS             2271..2516
                     /locus_tag="VBD85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            2513..2964
                     /locus_tag="VBD85_001153"
                     /pseudo
     CDS             2513..2964
                     /locus_tag="VBD85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            2961..3917
                     /locus_tag="VBD85_001154"
     CDS             2961..3917
                     /locus_tag="VBD85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7133712.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            4006..4383
                     /locus_tag="VBD85_001155"
     CDS             4006..4383
                     /locus_tag="VBD85_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7133713.1"
                     /translation="MISSIGQVMLYVSNVEASADFWKNKVGFERVEKQTQGDYVTYIV
                     APKLDSEVSFVLHDKAIIAQMSPELDLATPSILFETTDIDSTYQELTANEVMTNPIVD
                     MGSMRVFNFSDNDNNYFAIREVK"
     gene            4396..4809
                     /locus_tag="VBD85_001156"
     CDS             4396..4809
                     /locus_tag="VBD85_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7133714.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWSPAYANLVDQFGVMWQISTELN"
     gene            5053..6432
                     /gene="glmU"
                     /locus_tag="VBD85_001157"
     CDS             5053..6432
                     /gene="glmU"
                     /locus_tag="VBD85_001157"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO7133715.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            6453..7007
                     /locus_tag="VBD85_001158"
     CDS             6453..7007
                     /locus_tag="VBD85_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7133716.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            7007..7276
                     /locus_tag="VBD85_001159"
     CDS             7007..7276
                     /locus_tag="VBD85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133717.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            7286..7975
                     /locus_tag="VBD85_001160"
     CDS             7286..7975
                     /locus_tag="VBD85_001160"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO7133718.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMISKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(8015..8662)
                     /locus_tag="VBD85_001161"
     CDS             complement(8015..8662)
                     /locus_tag="VBD85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7133719.1"
                     /translation="MTPNKADYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            8829..9755
                     /locus_tag="VBD85_001162"
     CDS             8829..9755
                     /locus_tag="VBD85_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO7133720.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQTKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            9926..10642
                     /locus_tag="VBD85_001163"
     CDS             9926..10642
                     /locus_tag="VBD85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7133721.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            10644..11477
                     /locus_tag="VBD85_001164"
     CDS             10644..11477
                     /locus_tag="VBD85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7133722.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(11529..12332)
                     /locus_tag="VBD85_001165"
     CDS             complement(11529..12332)
                     /locus_tag="VBD85_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO7133723.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            12498..14948
                     /locus_tag="VBD85_001166"
     CDS             12498..14948
                     /locus_tag="VBD85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7133724.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            15030..16745
                     /gene="pabB"
                     /locus_tag="VBD85_001167"
     CDS             15030..16745
                     /gene="pabB"
                     /locus_tag="VBD85_001167"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO7133725.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
     gene            complement(16794..18139)
                     /locus_tag="VBD85_001168"
     CDS             complement(join(16794..17698,17698..18139))
                     /locus_tag="VBD85_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
                     /protein_id="HEO7133726.1"
                     /translation="MKLSYEDKLEIYELRKIGMSWSQISQRYDVRISNLKYMIKLMDR
                     YGVEIVEKGRNEYYPPELKQEMIDKVLIHGCSQLSVSLDYALSNCSILTNWLSQFKKN
                     GYTIVEKTRGRPSKMGRKRKKTWEEMTELERLQEENERLRTENAFPKKVEGFALEGRS
                     LTERTAETIREMVTGGFRLDLLLEITKIARATYYYQLKKLNKPNKDKAIKSDIQSIYD
                     EHRGNYGYRRIYLELRNRGFVINHKRVQGLMKSMGLTARIRRKRKYASYKGEVGKKAD
                     NLIQRQFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQ
                     TMLERAFPAASYSETILHSDQGWQYQHKSYHQFLEDKGIRPSMSRKGNSPDNGMMESF
                     FGILKSEMFYGLEKSYKSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFT"
BASE COUNT         5982 a         2711 c         3707 g         5849 t
ORIGIN      
        1 ataaaaaaat ggtttggggg catattacga gagagaaaca aatgaaggat tttgaaacaa
       61 gtgttactta gaagttatag cgattcatat atataaaata atcactaaaa gagcgtaatt
      121 atctacgctc ttttagtgat tatcaataat tggcctaatc tgaggttaat gtttaaaacc
      181 atctaaaatt ttagaaatat aatgataaat gagaaccaga actgttcaaa ataaaaatta
      241 atatcacgaa aatggtataa ttggagtagt tatcaggcag ttaataataa tttttgaaag
      301 gattttagtt atggcttgca taatcaggag agcaagatta ggagatgaag tcaatttagc
      361 ttatattcaa acggagagct ggaaagctgc ttttggtaag atactacctg aggatatcat
      421 tcaaaaaact acggaaatag aacctgccat aacaatgtat caacagctac tacacaagga
      481 ggttggtaag gggtatatat tggaagtaga tagcaatcct cattgtatgg cttggtggga
      541 taaaagtaga gaagatggta tgcttgatta tgcagaactc atttgtatac atagtcttaa
      601 agaaggttgg ggaaaaggtt atgggtctca aatgatgaat catgtactat cagagataca
      661 acaagcaggt tataataaag tgatactttg ggtatttaca gaaaatacac gcgcaaggaa
      721 attttacgat aggtttggtt tttcttttaa gggtaaatca aaaacttatt ttgaaaagga
      781 agaaatgtgt tatgagaaga ttttgcacta gcgagataaa gcatccgata atacaatagt
      841 ataaacatcc tattttgaaa aacaatttcc tttaagggga acaaatttgt aaaagaaaat
      901 tgttataggt ataatgaaga ttatgaaggt aaaatttttt gagtgaaaaa cagtgtatca
      961 attttaggtt ctctagtcta caaatagtta tgctaactct atggaagaat ggagaaaatt
     1021 acgaatgatt ttgaaaaagt tatagctgtg ctaaatcaac taagtatttc ttatgaatta
     1081 gtggaaaatg gacctgtttt gttgacggag caagcagata actttattgt gggaattgaa
     1141 ggagtaagaa caaaaataat gttcttgacc aacaagaaaa agacgaaata ttatttacta
     1201 ataatggatg accagaaact attttatcct atgaaaacaa tagtagcctt tagatgaact
     1261 aaaaggcgaa gaaactttaa attatgattt taaataagca ataaaccttg atatatcaat
     1321 ggttaaagac aataaaggag aacaatatga catttgaaga aattttacca ggtttaaaag
     1381 caaagaaaaa atatgtacgt acaggttggg ggggcgctga aaattatgtt cagttatttg
     1441 atacccttga agttaatggg aaagtactac aggctacacc ttattttctt attaatgtta
     1501 ctggtgaagg tgaaggtttt tccatgtggg caccaacacc atgtgatgtt ttagcagagg
     1561 attggatcga ggtcaatgac taaagttgtt ttagtgacgg gctgtgctag tggaattggt
     1621 tatgcacagg ctcaatattt tttgaaacaa gggtatcaag tatatggtgt ggataagtcg
     1681 gataaaccga acttaaatgg taattttaac tttataaagt tggatttatc ctctgattta
     1741 tccccactat tcacaatggt accgactgtt gatattttgt gtaatactgc aggtattcta
     1801 gatgcctaca agccacttct agaagtaagt gatgaagaac tggaacacct atttgatatt
     1861 aattttttcg tgactgttag gctaacacgt cattatctta gacgtatggt ggaaaagaag
     1921 tcaggtatta ttatcaatat gtgctcaata gcaagtttta tagcgggtgg cggtggagct
     1981 gcatatacta gttctaaaca tgcccttgcg ggattcacac gtcaattggc tttggattac
     2041 gcaaaagatt gtattcaaat ttttggtata gcgccaggtg ctgtacaaac tgctatgact
     2101 gctagtgatt ttgaacctgg tggattagca gaatgggtag ctagtgagac gccaattggt
     2161 cgttggacta aaccaagtga agttgctgaa ttaactggat ttcttgcttc aggaaaagcg
     2221 agatcaatgc aaggtgaaat tgtcaaaatt gatggtggct ggagtttgaa atgagaaaat
     2281 agattgaatt tggttgtggt aatacttggt tattacgtac ggaatttgaa gcatctgatg
     2341 gtactgagcg ggaagttaaa ggaatatcag gtgatatttt aataaaatct gtttatttac
     2401 gtgtttggat tggtcaaact gtttatattt tagatgttaa agaaggattt aaaagacaaa
     2461 gaaaaatgag taacgcagtt aagtttgttg tgggaattgc ttcggaggta atatgaaggc
     2521 acaagaatta tggaataagt ttaaatcttt gaatccttca atcggtgatg agatagacgc
     2581 atggcaattt ggagcagaac cagatcttct ggcaaagtta gttgtagatg ggacaaaaac
     2641 tgcaacggcc tctgcctatg atttatatga attagatgag gaagggttac acaagttggt
     2701 tcatatgata ttattttgga tagcaatgat gaagctatct gtattattga aataactaaa
     2761 gttgatattg tcccttttaa agatgtctct gcagaccatg cttttaaaga aggagaagga
     2821 gataaaactt tagaatggtg gagaaaagct cacattgatt tctttaaacc ttattttgag
     2881 gagtttggtt taatgtttag tgaggactct aggatagttt tagaggaatt ccaagtggtt
     2941 tacccaaagg agattaacga atgaaattag cggttttggg gacaggtatg attgttaagg
     3001 aagttttgcc tgtcttgcag aaaattgaag gtattgatct agttgctatc ttatcaacag
     3061 ttagaagttt ggagacagct aaagatctag ctaaggaata taacatgtct ctagctacga
     3121 gtgaatataa ggctgtcttg gacaatgagg agattgacac agtctatatt ggactgccga
     3181 atcacttgca ttttgactat gctaaggagg cactcttggc aggaaaacat gtcatttgtg
     3241 agaagccatt tacattagaa gcaagtcaat tagaagaact tgtatctatt gccaatacta
     3301 ggcaactaat attactcgaa gcaattacaa atcaatattt acctaatttt gaccttgtaa
     3361 aagaacattt aagcaacctt ggagatatta aaattgtaga atgtaattat tcccaatatt
     3421 catcgcgtta tgatgccttt aaacgtgggg aaatagcacc agctttcaat cctgaaatgg
     3481 gtggaggtgc gttgagagac cttaatattt ataatttaca tttagtaatt ggactttttg
     3541 gagaacctat aacagcacag tatttaccaa atatagagcg tggtattgat acttcaggag
     3601 ttctagtcct agactatggg cactttaaaa cagtctgtat tggagcaaaa gattgtagtg
     3661 cagaagtaaa atctactatt caaggtgata aggggtctat agcgatttta gggccaacta
     3721 atactatgcc taaaatttca ttaactatga atggacaaga aagtcatgtt tatcaattaa
     3781 atggtgatag gcaccgcatg cacgatgaat ttgttatttt tgaaggtatt attagcaatt
     3841 tagattttaa gcgtgctgct caagctttag aacatagtag aacggtgatg aaagttctag
     3901 atatggtaac gaaataagta aatgactctt ttgagtcatt ttttacttat tttaaatact
     3961 gtatgttata ctaattagta ataaaaccga aagtgagata ataaaatgat ttcatcaatt
     4021 ggacaggtaa tgctctacgt tagcaatgtt gaagcatcag cagatttttg gaaaaacaag
     4081 gtgggttttg agcgtgttga aaaacagaca caaggagatt atgtaaccta tatagttgca
     4141 ccgaagttgg atagtgaagt tagcttcgtc ttacatgaca aagcaattat agctcaaatg
     4201 agtcctgagc tagatttagc aactccctct attttatttg aaactactga tattgacagt
     4261 acatatcaag aattaacagc aaatgaggtt atgacaaatc ctattgttga tatggggagt
     4321 atgagagttt ttaatttttc cgataatgac aataattact ttgcaatccg tgaagtaaaa
     4381 taaaaaggag aacttatggt taaagcacta gaaacataca tcgtaacaaa tggaaatggg
     4441 cgacaagcgg ttgattttta taaagatgtc tttcaagcgg atttagtgaa tatgatgaca
     4501 tgggaagaga tggaccccaa ctgtcttgaa gaccgcaagg atttaatcat taatgctcag
     4561 cttatttttg atggcattcg tcttcaaatc tcggatgaaa atccagattt cgtgtatcaa
     4621 gctggtaaaa atgttacagc cgctattatt gtgggtagcg tagaggaggc gcgtgaaatt
     4681 tatgaaaaat taaagaagag tgcacaagaa gttcagttag agttacaaga aactttctgg
     4741 agtcctgctt acgcaaactt agtagaccaa tttggtgtga tgtggcaaat ttcaacagag
     4801 ttaaactaat cgtttttacc atctgagcat tgcaatgata gatttataaa aatactgatt
     4861 gcaaacagag aagaaaaaat taacggattt catgtattga atagctacca aaaggtagct
     4921 attttattcc taattgaagt aagctatttt agctaaagtc tattgtaata gggatttaaa
     4981 ggggtattag tgataagttg aaggcattat gatataatag gttgaattat attcagagat
     5041 agaggaatta atatgtcaaa ttatgccatt attttagctg cgggtaaggg aactcgcatg
     5101 aaatctgatt taccaaaagt tatgcataaa gtatctggta ttaccatgct tgagcatgtt
     5161 ttccgtagtg tgcaagctat tgagccaagt aaaattgtta ctgttattgg acataaggcg
     5221 gagcttgtac gtgatgtttt aggtgacaaa tcagagtttg taatgcaaac agaacagcta
     5281 ggaactggcc atgctgtaat gatggcggaa gaagaattag cgacatcaaa aggacataca
     5341 ctagttattg caggtgatac acccctgatt actggagaaa gtttaaaaaa cttaattgat
     5401 tttcatgtca atcataaaaa cgttgcaaca atcttaacag ccgatgctgc aaatccattt
     5461 ggatatggtc gtattatccg taattcagat gatgaagtta ctaaaattgt agaacaaaag
     5521 gatgctaatg actttgagca gcaggttaaa gagattaata ctggaactta tgtttttgat
     5581 aatcaaagtc tatttgaagc acttaaagac attaatacaa ataacgctca aggagagtat
     5641 tatctaactg atgttattgg aattttcaaa gaagcgggta aaaaggtagg tgcctataaa
     5701 cttcgtgact ttgacgaaag tctaggagtt aatgatcgtg tagcacttgc aactgctgaa
     5761 aaagtgatgc gtcaccgaat tgcacgtcaa catatggtta atggggtcac agtggttaat
     5821 cctgattccg cttatattga tattgatgtt gagattggag aagaatctgt aattgagcct
     5881 aatgtgactt taaaaggaca gacgaagatt ggtaaaggta ctttattaac caatggtagt
     5941 tatttagtag atgctcaagt cggaaatgat gttaccatca ctaattcgat ggttgaagaa
     6001 tccattattt ctgatggagt aaccgtaggc ccttatgcgc atattagacc aggaacaagt
     6061 cttgcaaaag gtgttcatat tggtaatttc gttgaagtaa agggttctca aataggtgag
     6121 aatacgaagg caggtcattt aacttatatc ggcaatgcag aggtaggttg tgatgttaac
     6181 tttggcgcag ggacgattac tgtcaattat gatggacaaa ataaatttaa aacagaaatc
     6241 ggaagtaatg tctttatcgg aagtaattct actctgattg caccacttga aattggtgat
     6301 aatgctttaa cagcagctgg atctaccatc acagataatg ttccaattga tagcatagct
     6361 attggaagag gacgtcaagt caataaagaa ggatatgcca ataaaaaacc acatcatcct
     6421 tcacaaaagt aacacttatt tgggaggttc ttatggattt tgaagaaaaa acaattaatc
     6481 gtcaaactgt ttttgatgga caaattatca aagtagctgt tgatgatgta gagttgccaa
     6541 atggattggg acaatccaaa cgtgaacttg tttttcatgg aggagctgtt gctactttgg
     6601 cggtaactcc tgagcataaa attgttttag tgaaacagta tcgtaaggct attgaaggta
     6661 tttcttacga aattccagca gggaaacttg agacgggtga gtcaggttct aaggaagaag
     6721 ctgcccttcg ggaattagaa gaagagactg gctacacagg gaatcttgaa attctttata
     6781 gtttctacac tgctattggt ttttgtaatg agaaaatcgt cctctatcta gctactgatt
     6841 tacaaaaagt tgaaaatcct aggccacaag atgatgatga ggtccttgag cttctagagt
     6901 taagttatga ggattgtatg caaatggtag aaaaaggcat gattcaagat gcgaaaacga
     6961 tcattgcttt acaatattac ggtttaaaaa tgggaggtca ttcttaatgg gaaaaccatt
     7021 gttaacagat gatatgattg aacgttcaaa tcgaggtgaa aaagtttcag gtcaaacaat
     7081 cttagatcaa gaaactaaaa ttatttctac agaagatggc atggagcaac taactgacga
     7141 gaacggtaaa catatctaca agagtcgccg gattgagaat gcaaaacgaa atgaatttca
     7201 acgtaagttg aatttagttt tattcatttt gcttattttg ttggctctct tgttttatgc
     7261 aatttttaaa ttataaggaa atatcatgaa aattggaatt attgcagcca tggaggaaga
     7321 gttaaaactc cttgtggaaa atcttgagga taaatctcaa gagacagtat tatcaaatgt
     7381 atattacagt ggacgttatg gtgaacatga gcttgtttta gtacagtctg gtgttgggaa
     7441 agtcatgtca gcgatgagtg ttgctatttt agtagaaagc tttaaagttg atgctattat
     7501 taatacagga tcagctggtg cagtagcaac aggattgaat gtaggagacg tcgttgtcgc
     7561 agatacatta gtttatcacg atgtagattt aaccgccttt ggttatgact atggacaaat
     7621 gtcaatgcag ccactttatt ttcattcgga taagactttt gtttccactt ttgaagcagt
     7681 tttatctaaa gaagaaatga ttagtaaagt tggtttaatt gcaactgggg atagttttat
     7741 tgctggccag gaaaagattg atgttattaa ggggcatttt ccacaggttt tagctgtcga
     7801 gatggaaggt gcagcaattg cccaagctgc tcaagctaca ggaaagccct ttgtggtggt
     7861 acgtgctatg agtgatactg cagctcatga tgccaatatc acatttgatg aatttatcat
     7921 tgaagctggt aaacgctctg cccaagtatt aatggcattt ttgaaagcat tataaaaaag
     7981 cccgtgtatc taatgaatac acggactttt ttatttatgt agcagataca tatatttgac
     8041 ttgcgatacg ctcagggatt gttagatttt catcgttata ggtgatgtga taagtttttg
     8101 cataagggtc aatttgtgtc aattcaatgg tatcgttgat atgcaaacgg tgttgttcta
     8161 ggtatttcag tagctgaaaa tcatcatgaa cacgactgat aacatatgtg ccaagctgac
     8221 taatttggtc caatgtcatt tggttaattt ctactaaaaa ctctcccttt ttaggaatag
     8281 tcccaccgtg tggacaaaat tgtggaaagt ctaacaactt ttctaattga tcaacaaaag
     8341 ttgttgatac agtatgttct aaaacttccg cttcttcatg aatatcatca gcagtatagt
     8401 gcaaatggtt aactaaaaat acctctacta gacgatgttt acggtaaagg tcagaaacaa
     8461 ctaataaacc ttgtttggta agataatatc ctaaatcttt atctttaaca atcagttgtt
     8521 cacttatcat ttttttaatc atttctgaca cggcaggagc cgagacttgc atcatttcag
     8581 cgattttctt attggaaatt ttgttgtttt tttcacctaa ttcatgaatg cattttaggt
     8641 aatctgcttt atttggagtc atattcagtc tttcttatac ttcttaaaat ctatttttct
     8701 aagaccatta tatcaaaaaa tactgaaaaa gtatttgaca aatactttat aataggttat
     8761 gctaatatca aaattaagtt aacttaattt aatacataac ttatattaat tttaaaagga
     8821 gcattttgat gaaaaagtgg ttagttattg tatcatgttt tgtagcattt ttaggcttag
     8881 gtgcctgtgc taacaagcag gatcaaacca aaaacgaaaa attaaaagta gttgtaacca
     8941 actctatttt ggcagatata acaaaaaata tagccggtaa caaaatcgac ttacatagta
     9001 ttgtacctgt tggtaaagat cctcatgaat atgagccatt accagcagac gttgaaaaaa
     9061 caactcaagc agaccttgtt ttttacaacg gaattaatct tgaaacaggc ggtaatgctt
     9121 ggtttacaaa attaataaag aacgctaaga agaaagaaaa caaagattac tttgcggtta
     9181 gcgatggagt tgatgttatt tatctaaatg gtcaaagcgg aaaaggtaaa gaagacccac
     9241 atgcttggtt gaaccttgaa aatggtatta tttactcaaa aaatattgct aagcaattaa
     9301 ttgctaaaga tccaaaaaac aaagctactt atgagaaaaa cagagatgct tatgtagcta
     9361 aattagaaaa attggataaa gaagcaaaat ctaaatttaa tgccattcca gctaataaaa
     9421 aattaattgt tactagtgaa ggttgcttta aatatttctc aaaagcttat ggagttccat
     9481 cagcatacat ctgggaaatt aacactgaag aagaaggtac accagatcaa attactagct
     9541 tagttaagaa attaaaacaa gtaagacctt cagctttatt cgttgaatca agtgttgaca
     9601 agcgtcctat gaaatctgtt tcaagagaat caggtatccc aatttacgca gaaatcttca
     9661 cagactcaat tgctaaaaaa ggtcaaaagg gtgacagcta ctacgctatg atgaaatgga
     9721 atcttgataa gattgcagaa ggtttagcaa aataagagtg agaatcgaca ttgaaatcat
     9781 aatgaaatca atgatgtagt attctcgctt aaggctgagt tgtaacaatg tcgaaatcct
     9841 aatctaaaaa ctgacttagc tttaataaat acagagaagt ggagtctggt tttataacca
     9901 gctccattct acttttggag gaaaaatgat tattagtaaa cacttaagcg taagttatga
     9961 taataattta gtgctagaag atattaattt acgcttagaa ggctccggaa tcattggtat
    10021 tttagggcct aatggggcgg ggaaatctac gcttatgaaa gctcttcttg gtttggttga
    10081 ttctactgga gaatcaggga ttgggggaga tttgctacct ttaatgggga gagtagcata
    10141 tgttgaacaa aaaactaata ttgattacca attccctata actgttggag aatgcgtttc
    10201 tttaggatta tataaggaac gaggcctctt caagcgacta tcaaaaactg actgggaaaa
    10261 agtatcccga gtgattgatc aagttggttt gagagggttt gaaaatagac caattaatgc
    10321 cttatcaggt ggacaatttc aacgtatgct tatggcaaga tgtctcgttc aagaagcgga
    10381 ttacattttt ttagatgagc cgtttgtagg gattgattcc attagtgaac aaatcatagt
    10441 taatttacta aaaaaattaa gtaaggccgg aaaattaatt ttagtcgtcc atcacgattt
    10501 gagtaaagtt gatcattatt ttgatcaagt aattattttg aaccgtcatc taattgcttg
    10561 tggaccaata gatcaggcat ttacccgtga gaatttaagt gctgcttacg gtgatgctat
    10621 tttgttagga caaggagact agaatgttta cgaaattttt tgaaggctta ttgacttatc
    10681 attttttgca aaatgctttt ataactgcaa ttgtgattgg tattgtagcg ggtgcagtag
    10741 gctgttttat tatcttgcgc tcaatgtctt tgatgggtga tgctatttct cacgctgttc
    10801 taccaggtgt tgctatatct tttattttag ggattaactt ttttataggt gctattgtct
    10861 ttggtttgtt gtcttcgatt atcattactt atattaaaga aaatagtgtc ataaaaggag
    10921 atactgctat tggaattact ttttcgtcct ttttagcctt aggtatcatt ttaattggac
    10981 ttgctaatag tacaactgac ctctttcata ttctttttgg gaatattttg gcagtacaag
    11041 atagtgataa atatatgact attattgtgg gattaattgt actgactctg attactatct
    11101 tctttaaaga attattattg acttcttttg atcctgtttt agcaaaatca atgggaatga
    11161 gagtttcttt ttatcattat ttattgatga ttttgttaac attagttgca gtaacagcta
    11221 tgcagagtgt gggaacaata ttgatcgtag cacttttaat tacaccagct gcaacagcat
    11281 atctttatgt taaaagtctc agaacaatgt tgtttttatc atctgcatta ggagcagtag
    11341 catctgttct ggggttatat atcggttata catttaatat tgccgcagga tcaagtattg
    11401 ttttaacatc tacttttatg tttttacttg ctttcctatt ttctcctaaa caaagtttgt
    11461 ttaaaaagaa acaatagtgt tgttaaaaaa tgagactaga aaatgttcta gtctcatttt
    11521 tgatagaatt atttaaattt gtagtcttta ataactttga tacttgtaat agtaattttt
    11581 tcttttggtt gatctgattt tgtaacttct accgaagcaa ttttatccac agtttccatg
    11641 ccagagatta cttgtccaaa gacggtataa ccaccatcta aacttggatt tcctccctct
    11701 gaataggctt taataatcac cttaggaact tttttatctg acagttgttt ggaatgatct
    11761 tgctggcttt gattgataaa gaattggctg ccattagtat cagctccagc atttgccatt
    11821 gctaaacttc ccctgatatt gtagaggtaa ggggatattt cattaacaaa accatttcca
    11881 gaatctttct ttttatcttt gctattccat atagattttc caccggtccc gtctccatta
    11941 gggtcaccag attgtatcat aaaatctttg ataactctgt ggaaacttaa cccattatag
    12001 tatccttctt ttgcatgtgt tagaaaattt tcaacagcta aaggagcata ttttggaaat
    12061 aacttgatat ttatatcacc tttagaggtt tttataagca cttgtgcctc gtttttggca
    12121 acagccttgt ccaattgagg aaaagctttt ttatcagctt ttaaggcttg cttagttttt
    12181 ttgtttagtt gttcagtagc ttcctttgaa gagttttcag ctagtttttg gtcaacataa
    12241 cggtctcctt ttagagaacg ctcaattgac tcgcaaccac ttaaagttaa tatactgaca
    12301 caggctaaac caagataaat aatttttttc atacatactc cttgattaag ttattaagat
    12361 gattatattt tatcataaat ttttaaataa tttataaaag taaatagtaa acctattggg
    12421 ggacttgttg aaattatgat agaatagtat agcatgagtt gatagagaaa gtgaggaaca
    12481 agtatttcac ttgaaatatg gtatttatgg caaataagaa aaaaacaaaa ggaaagaaaa
    12541 ccagaagacc tactaaggca gaaatagagc gtcaaagagc tattcaaagg atgattactg
    12601 ctcttgtttt aacaattatt ctcttctttg gtattatcag attaggtatt tttggtatta
    12661 cagtctataa cgtcatccgt tttatggtag gtagcttggc ttacttattt attgcggcaa
    12721 ctttaatcta cctttatttc tttaaatggt tgcgaaagaa agatagctta gtagcaggtt
    12781 ttttgatagc ttctttagga ttattgattg agtggcatgc ttaccttttc tcaatgccta
    12841 ttttgaaaga taaagaaatt ttgcgttcaa ctgctcgatt aattgtgtct gatttaatgc
    12901 aatttaaaat cactgttttt gccggtggag gtatgttggg tgctttgatt tacaagccaa
    12961 ttgcttttct cttttctaat attggtgcct atatgattgg tgttctcttc atcattttgg
    13021 gtctcttttt aatgagttct ctggaagttt atgacatcgt cgaatttatt agagctttta
    13081 aaaataaagt ggcagagaag cacgagcaaa ataaaaagga gcgttttgct aagcgagaga
    13141 tgaaaaaagc aatcgctgaa caagagcgca tagagcgtca aaaagctgaa gaagaagctt
    13201 atttagcttc ggttaatgta gaccctgaaa cgggtgagat tctagaggat caagctgagg
    13261 acaatttgga tgatgcgcta ccacctgagg taagtgaaac atcaactccg gtatttgagc
    13321 cagagatcct tgcttatgag acatcgcctc aaaatgatcc tttaccagta gagccgacaa
    13381 tttatttaga agactatgat tcgccgattc ctaatatgag agaaaatgat gaggaaatgg
    13441 tttatgattt agatgatgat gtagatgata gtgatataga aaatgtcgac tttacaccta
    13501 aaacgacact ggtttataaa ttaccaacga tagatttatt tgcaccagat aagcctaaaa
    13561 atcaatccaa agaaaaggat ttagtccgaa agaatatcag agttttagaa gaaacattta
    13621 gaagttttgg tatcgatgta aaagtagaac gtgctgaaat tggaccatca gttactaaat
    13681 atgaaattaa accagcagtt ggagttcgtg tgaatcgtat ttcaaatcta tctgacgacc
    13741 tagctcttgc tcttgcagca aaagatgtgc gtatagaagc accaattcct ggaaaatcat
    13801 taataggtat tgaagttcct aactcagaaa ttgcaacggt ttctttccgc gaactttggg
    13861 aacaatctga tgccaatcct gaaaaccttt tagaagtacc actaggaaaa gctgttaacg
    13921 gcaatgctcg cagttttaac ttagctagaa tgccgcatct tttggtagct ggttcaactg
    13981 gttcaggtaa atctgtggca gttaatggaa ttatttcaag tattttgatg aaggcacgtc
    14041 cagatcaagt taagtttatg atgattgatc ccaaaatggt tgaattatct gtttataatg
    14101 atattccaca tttattaatc cctgttgtaa ccaatccgcg taaagcaagt aaggcactcc
    14161 aaaaagttgt tgatgaaatg gaaaatcgat acgagttatt tagcaaaatt ggtgtgcgta
    14221 atatagcagg ttataataca aaggttgaag agtttaatgc ttcctctgag caaaaacaaa
    14281 tacctttgcc tttaatcgtt gtcattgtag atgaattggc tgacttgatg atggttgcta
    14341 gtaaagaagt tgaagatgct attattcgtt tggggcaaaa agcacgtgct gcaggtatcc
    14401 atatgattct tgcaactcaa cgtccatccg tagatgttat ttctggtttg attaaagcaa
    14461 atgttccgtc gcgtattgca tttgctgttt caagtggtac tgatagccgt acgatccttg
    14521 atgaaaatgg tgctgaaaag ctcttgggac ggggtgacat gctctttaag cctattgatg
    14581 agaatcatcc agtacgacta caaggttcct ttatttcaga tgatgatgtt gaaaggatcg
    14641 ttggttttat caaagaccaa gccgaggctg actatgatga tgcctttgat cctggagaag
    14701 tatctgaaac agataacggc tctggtggtg gcggcggagt acctgaaagt gatcctcttt
    14761 ttgaagaagc caagggactc gttttagaga cgcaaaaagc aagtgcctca atgattcaac
    14821 gccgattgtc tgttggtttc aatagagcaa caagactaat ggaagaatta gaagcagcgg
    14881 gggttattgg tccagcagaa ggaaccaagc cacgaaaagt tttaatgact ccaactccga
    14941 gtgaataaga aaaaactaat ctgacaagaa attgtcggat tagtttttta tgttataatg
    15001 aagcaaatag atgaagaaga aggttacgaa tgcatattga gactgttatt gatttcaaag
    15061 aattaggaaa aagatatcgt tttaaaaatc ctacaaaaga attaatagct gatactttag
    15121 aacaagtctt agaagtgata aaagaagttg attattatca atctcaaaat tattatgttg
    15181 ttggttattt atcttatgaa gcatctgctg cttttgattc acattttaaa gtttctcaac
    15241 agaagttggc tggagaacat ctagcttatt ttacagtaca taaagattgt gagaacgaag
    15301 cttttccttt aagttatgaa aatgttagat tagcagataa ttggactgct aatgtttctg
    15361 agcaagaata tcaagaggca attgctaata ttaaaggaca aattagacaa ggaaatactt
    15421 atcaagtaaa ttatacacta gagcttagcc aacaattatg ctcggatccc tttagtgttt
    15481 atgaacgcct aatggtagaa caaggagcag gatataatgc ttatattgcc tacgacgata
    15541 aaaggatttt atctgtaagc ccggaattgt tctttaagaa gaaggatgaa gtcttaacaa
    15601 ctagaccaat gaagggaaca agcgctagaa agcctactta tcaagaagat gtggcggagc
    15661 gagattggtt ggcaaatgat cctaaaaatc gttctgaaaa tatgatgatt gttgatttat
    15721 taagaaacga tatgggccgt atctgtgatg ttggaacagt taaggtaaaa aaactatgtc
    15781 aagtggagca gtatgcaact gtgtggcaaa tgacatcaac tattgaagga gttttatcgc
    15841 cagaagtgac acttatgtct atttttcaag ctctatatcc ttgtggatct atcactggag
    15901 ctcctaagat atcaacaatg gctattatta atgagttgga aaaacggcca agaggtattt
    15961 attgtgggac gataggactt tgcatgccag acggacaagc tatttttaac gttcctattc
    16021 gcacagtaca aatgaaaggt caacaagcct attacggcgt tggtggaggt attacgtggg
    16081 agagccagac agattctgaa tatgaagaaa cccgtcaaaa atcagctgtt ttaacacgtg
    16141 tcaatccaaa atttcaatta ataactacag gaagagtgac tgaaaataaa cttcttttct
    16201 ctcaacaaca tgtcgagaga ttagttgaat cagcgtctta ttttgcttat tcttttgata
    16261 aaagtaaatt cgaaagggaa ctgaaaaaat accttcatca gctagatgag aaggattacc
    16321 gcttgaaaat aatgcttgat aaaactggta aggtaacgtt tgaggtaaaa caactagtga
    16381 atttatcaaa aaagttttta acggcagagg tggtcgtaca agactaccct attaaattat
    16441 ccccgtttac ttattttaaa acttcttatc gcccacatat tattgaaggt cagaatgaaa
    16501 agatatttgt atctcctgag gggttgctat tggaaacaag tattgggaat attgttttag
    16561 aaaaaaatgg aaggttttta accccagatt tatcagaagg agggttgaat gggatttatc
    16621 gtcgtcatct ccttaaaaac caaaaagtaa ttgaagcacc actaacttta aaagatttag
    16681 aatcagccga tgctatatac gcctgtaatg ctgttagagg gctttatcct ctaaacctaa
    16741 agtaaaaaga gttggaaatt ttgtactgac ccccaaaagt tggacaattt attttaagtg
    16801 aaggatttag ttctgtattg cacaggacta agtcctttta gttttgcttt gattcgcttg
    16861 ttgttgtagt aaaaaatgta atctgtgata gcttgctcaa ggtcatcaag tgatttgtaa
    16921 gacttctcca agccatagaa catctcagat tttagaatcc caaagaaaga ttccatcata
    16981 ccgttgtcag gactgttccc cttccgtgac atagaaggac gaatgccttt gtcctccaga
    17041 aattgatggt aggacttgtg ctggtactgc cagccttggt cgctgtggag aatggtttcg
    17101 ctgtatgaag ccgcaggaaa agccctctca agcatggttt gaacctgttt caagtcagga
    17161 gaccgagaca gggtaaaatc aattatctca ctgttatagc catcaagaac aggcgataga
    17221 taaagtttcc cttcaggtaa agcaaactcc gtcacatcgg tatagcactt ctcatagggc
    17281 ttcgacccct caaactggcg ttgaatcagg ttatcagcct tcttaccaac ctcacctttg
    17341 taagaggcat acttgcgctt acgacgaata cgagcagtca agcccatact tttcatcaag
    17401 ccttgcaccc ttttgtggtt gatgacaaag ccacgatttc gcagttctaa ataaatccga
    17461 cgatagccat agtttcctct atgttcatca taaatggatt gaatgtcaga tttgattgct
    17521 ttgtctttat ttggtttatt cagtttcttt agttgataat agtaagttgc acgagctatc
    17581 ttagttatct caagtaagag gtctagtcgg aatcctcctg tgaccatttc tctaattgtt
    17641 tctgccgttc gctctgtaag gcttcgtccc tcaagcgcaa atccctcaac ttttttagaa
    17701 aggcattctc cgttctcaag cgctcatttt cttcctgaag tcgctcgagc tctgtcattt
    17761 cttcccaagt tttcttacgc ttacgtccca ttttactcgg tctccctctt gttttctcaa
    17821 caatagtata accatttttc ttaaattggg aaagccaatt tgtgagtata gaacaattag
    17881 agagagcata atcaagagaa acagagagtt gagaacaacc atgaatcaag actttatcta
    17941 tcatttcctg ctttaattca ggtggataat actcatttct acctttttcg acgatttcta
    18001 caccataccg atccatgagt tttatcatgt atttaagatt cgagatacga acatcatatc
    18061 gttgactaat ctgagaccag gacatcccga tctttcttaa ctcgtatatt tctagtttat
    18121 cttcataact taatttcatc agaaaagcac cccaatcgtt agattttttg tctaactttt
    18181 ggggtgcagt tcaatttatc caactctttt tattgtactt tgataaagat gataaccaat
    18241 aatcctagg
//
LOCUS       DATRSD010000042        18234 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRSD010000042 DATRSD010000000
VERSION     DATRSD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18234
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.042"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            256..570
                     /locus_tag="VBD85_001169"
     CDS             256..570
                     /locus_tag="VBD85_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7133727.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            586..972
                     /locus_tag="VBD85_001170"
     CDS             586..972
                     /locus_tag="VBD85_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7133728.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1001..2386
                     /locus_tag="VBD85_001171"
     CDS             1001..2386
                     /locus_tag="VBD85_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7133729.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2389..2541
                     /locus_tag="VBD85_001172"
     CDS             2389..2541
                     /locus_tag="VBD85_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7133730.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2564..3769
                     /locus_tag="VBD85_001173"
     CDS             2564..3769
                     /locus_tag="VBD85_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7133731.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3812..4033
                     /locus_tag="VBD85_001174"
     CDS             3812..4033
                     /locus_tag="VBD85_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133732.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4150..4647
                     /locus_tag="VBD85_001175"
     CDS             4150..4647
                     /locus_tag="VBD85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7133733.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4622..5128
                     /locus_tag="VBD85_001176"
     CDS             4622..5128
                     /locus_tag="VBD85_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7133734.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5112..7559
                     /locus_tag="VBD85_001177"
     CDS             5112..7559
                     /locus_tag="VBD85_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7133735.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7562..9739
                     /locus_tag="VBD85_001178"
     CDS             7562..9739
                     /locus_tag="VBD85_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7133736.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9736..10737
                     /locus_tag="VBD85_001179"
     CDS             9736..10737
                     /locus_tag="VBD85_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO7133737.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10734..11666
                     /locus_tag="VBD85_001180"
     CDS             10734..11666
                     /locus_tag="VBD85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7133738.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11911..12027
                     /locus_tag="VBD85_001181"
     CDS             11911..12027
                     /locus_tag="VBD85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7133739.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12043..13962
                     /gene="tet(M)"
                     /locus_tag="VBD85_001182"
     CDS             12043..13962
                     /gene="tet(M)"
                     /locus_tag="VBD85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7133740.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14081..14248
                     /locus_tag="VBD85_001183"
     CDS             14081..14248
                     /locus_tag="VBD85_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7133741.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14308..14661)
                     /locus_tag="VBD85_001184"
     CDS             complement(14308..14661)
                     /locus_tag="VBD85_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133742.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15166..15588
                     /locus_tag="VBD85_001185"
     CDS             15166..15588
                     /locus_tag="VBD85_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7133743.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15585..15815
                     /locus_tag="VBD85_001186"
     CDS             15585..15815
                     /locus_tag="VBD85_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133744.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16276..16479
                     /locus_tag="VBD85_001187"
     CDS             16276..16479
                     /locus_tag="VBD85_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7133745.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16561..17778
                     /locus_tag="VBD85_001188"
     CDS             16561..17778
                     /locus_tag="VBD85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7133746.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         5988 a         3240 c         3770 g         5236 t
ORIGIN      
        1 actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa aagtgaagat
       61 ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa aaaatgaggt
      121 gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa aatcaagaat
      181 caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa taaaaaagaa
      241 aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa aacattcggc
      301 aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg acggctaact
      361 gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga tattgtggtg
      421 gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt gaagttagtc
      481 aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac aaattacctt
      541 ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat gagattagca
      601 aatggcattg tattagataa agacacgact tttggagaat tgaaattctc tgctctacgt
      661 cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa ggaacgtacc
      721 tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc tgccagcgtg
      781 cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat tgcggacacc
      841 gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc agacgatatt
      901 gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa agaaccgaca
      961 caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc gtggtaaaag
     1021 gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac tcctgcctgt
     1081 tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca actggcagga
     1141 ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca gtttcagtgt
     1201 cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct atgatacggt
     1261 taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca agtggtatga
     1321 atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta caaaggaaaa
     1381 gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac agatacgggt
     1441 ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga aattagagag
     1501 tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg aatatacttt
     1561 gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta aagatggtaa
     1621 acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata tgttgattgc
     1681 tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag ccttgcttca
     1741 tacagattca aaactgtata ttcttgaccc gaaaaatgct gatcttgcgg acttaggttc
     1801 tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg aaacattcta
     1861 tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata agactggcaa
     1921 aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat acgtcgcttt
     1981 catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga aacagattgt
     2041 catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc cagacgcaaa
     2101 atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag gtcgtatgtc
     2161 tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct tcttaaagcg
     2221 aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt tttatactcc
     2281 ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca acagcagaca
     2341 gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt gctggctggt
     2401 gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta caaatcgaca
     2461 ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag
     2521 cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact
     2581 gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca
     2641 aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg
     2701 aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa
     2761 tcccgacgct ccccttgaaa tgctgtttga ttatgtaaga attcgctttc cgacaacaga
     2821 cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga
     2881 ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc
     2941 ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga
     3001 aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc
     3061 tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttaaatat
     3121 ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa
     3181 aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct
     3241 ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca
     3301 gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg
     3361 attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga
     3421 gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa
     3481 acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga
     3541 acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct
     3601 atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac
     3661 gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt
     3721 gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac tcaaatacaa
     3781 attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc tttataactg
     3841 gtttctatca aacgctcaat cactggtgct tttagcaatc gttgtgattg gcttgtatct
     3901 tggcttcaag cgtgagttta gcaaactgat tggcttttta attattgcga ttattgcggt
     3961 tggcttagtc ttcaacgctg ctggagtaaa agacatttta ctagagctat tcaatcgcat
     4021 tattggtgct taaataaaac cgttcttttg tggaatataa gtggttttct tatgttccgc
     4081 aaaggaatgg tacaccaaac gaagtgcggt agggattttt gaatctctac aaagaaagga
     4141 cgtgaatata tggacgatat gcaagtctat attgcgaatt taggcaaata caatgagggc
     4201 gaattggtcg gtgcgtggtt tacctttccc attgactttg aggaagtcaa agagaaaatc
     4261 ggcttgaatg atgaatatga ggaatacgcc attcatgact acgagttacc ctttacggtt
     4321 gacgaataca cttccattgg cgaactcaat cgactatggg aaatggtatc ggaattaccc
     4381 gaagaattac aatcggagct atctgctctg ctcactcatt tttcaagcat tgaagaacta
     4441 agcgaacatc aagaggatat tatcattcat tccgattgtg atgatatgta tgacgtggca
     4501 cgctactaca ttgaagaaac gggtgcttta ggcgaagtac cagctagtct tcaaaactat
     4561 attgattatc aagcctatgg tcgggattta gacctttcag gaacgtttat ctcaaccaat
     4621 catgggattt ttgaaatcgt ctattaaatc tgtcggtaca ttactactgg cagattttct
     4681 attttacggg gtggctcaat cagctacccc tattttttat gaaaggattg attacatgaa
     4741 gaaaatacga agctatacca gtatctggtc tgtggaaaag gtactgtatt ctatcaatga
     4801 ttttagactt ccgtttccca taacctttac gcaaatgaca tggtttgtcg tgtcactctt
     4861 tgcagtgatg atacttggca acttgccccc tctttccatg atagagggag catttctcaa
     4921 atactttggg attcctgtgg ctttcacatg gtttatgtct acaaaaactt ttgatggtaa
     4981 aaagccttat ggatttttga agtctgtcat tgcttatgca ctgcgaccaa agctgaccta
     5041 tgcaggaaaa aaagtaacgc ttggcagaaa ccagccacaa gaagccatta cagcagttag
     5101 gagtgaattt tatggcatat ccaattaaat acattgaaaa caatctcgtc tggaataaag
     5161 acggggaatg ttatgcttac tatgagcttg ttccttacaa ttactcattt ctaagtccag
     5221 aacagaaaat acaagtgcat gattctttca gacagcttat cgcacaaaat cgtgatggca
     5281 aaattcatgc tttacaaatc agtacagaat ccagcatacg ttctgcacaa gagcgttcca
     5341 aaaatgaagt cactggcaag ctcaaagcgg ttgcctatga caaaatcgac caacagacag
     5401 acgctttaat atccatgatt ggcgaaaatc aagtgaacta ccgtttcttt atcggcttta
     5461 agttgcttct caacgatcag gagttttcta tgaaaagtct taccgttgaa gcaaaaaatg
     5521 ctttgtctga ttttgtctat gatgtgaacc ataagctgat gggcgatttt gttagtatga
     5581 gtaatgatga aatcctgcgt tttcagaaga tggaaaagct cttagaaaat aaaatctctc
     5641 gtcgtttcaa aatccgcagg ttagataagg acgacttcgg ctatctgatt gaacaccttt
     5701 acggacagac aggcactgcc tatgaagagt atgagtacca tctatcaaag aaaaagctgg
     5761 ataatgaaac gctgattaaa tactatgact tgattaagcc tactcgctgt ttggtggaag
     5821 aaaaacagcg atatttgaaa atccagcagg aagatgaaac cgtctatgta gcttacttta
     5881 ccattaacag cattgtcgga gaactggact tcccgtcctc tgaaatcttc tactaccagc
     5941 aacagcaatt tacattcccg attgatacgt caatgaatgt ggaaattgta gcgaatcgta
     6001 aagccctatc tactgtccgc aataaaaaga aagaactgaa agacttggat aaccacgctt
     6061 ggcaaagtga taatgaaacc agctccaatg tggcggaagc tctggaaagt gtgaatgagc
     6121 tggaaaccaa tttagaccaa agcaaggaat ctatgtacaa gctgtcttat gtggtaaggg
     6181 tatcagcaaa tgatcttgac gaactcaaac gtcgttgtaa tgaagtgaaa gatttttatg
     6241 acgatttaag cgtaaaactg gtacgaccat ttggggatat gctcggctta catgaagaat
     6301 ttttacctgc cagcaagcgt tatatgaatg attatattca atacgtgacc tctgatttcc
     6361 tcgctggttt aggttttggt gctactcaaa tgctggggga aaatgagggg atttatgttg
     6421 gctacagctt agatactgga cgcaatgtct atctgaaacc tgctcttgcc agtcaagggg
     6481 ttaagggttc agtaaccaat gcgttagcgt cggcttttgt tggttcgctg ggtggtggta
     6541 aatcctttgc gaataacctt atcgtctatt atgcggtgct ttatggggca caagcagtga
     6601 ttgtagaccc aaaagcagaa cgtggcagat ggaaagaaac cttgccagag atttcccatg
     6661 aaatcaatat cgtcactctg acttctgatg agaaaaacaa aggcttactt gacccttatg
     6721 tgattatgaa aaatcccaaa gattctgaat cactggctat tgatattctg acattcctta
     6781 cggggatttc ctctcgtgat ggggaacgct tcccaatcct tagaaaagcc attcgtgcag
     6841 taaccaatag tgaagtacga gggttgatga aagtgattga ggaattacgg gttgagaata
     6901 cgccactaag taccagtata gccgaccata tcgaaagttt tacagactat gactttgcac
     6961 atttattatt cagtaatggt tatgtggagc agtctatcag cttagaaaaa caactgaaca
     7021 ttatacaggt tgcggacttg gtacttcccg acaaggaaac ttcctttgag gaatatacca
     7081 ctatggagct tttatccgtt gctatgctga ttgtcattag tacctttgct ttagacttta
     7141 tccatacaga ccgaagcatt ttcaagattg tagatttaga cgaagcatgg agctttttac
     7201 aggtagcgca aggaaaaaca ctatctatga agctggttcg ggctggtcgt gctatgaacg
     7261 ctggggtata tttcgtgacc caaaatacag acgacctctt agatgaaaaa ctgaaaaata
     7321 acctcggctt aaaatttgca tttcgttcca ctgaccttaa cgagattaaa aagaccttag
     7381 ccttttttgg tgtagaccca gaggacgaaa acaatcagaa gcgattgcgt gatttggaaa
     7441 acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg tgtgatacag ttccaccctg
     7501 tatttgaaga actgctccat gcctttgata ccagaccacc tgtgcgaaaa gaggtgtaaa
     7561 tgtgaaacca tcaatagtaa acagaataaa atcaaactgg acgctgaaac gtctaggtaa
     7621 agtggcaatg acagtggctt tcacacttgt gattgccatt tttcttttag ccatgctggg
     7681 aacggtggtt caagctgcgg gcttggtaga tgatacggtc aatgtggcaa atgaatacag
     7741 ccgataccca cttgaaaact atcaactgga tttttatgtg gataatagct ggggctggct
     7801 tccgtggaac tggtcggacg ggattggaaa acaggtcatg tatggactat atgccattac
     7861 caattttatt tggacaatca gtttgtatgt ttccaatgcg acaggttact tagtacagga
     7921 agcctattcc ttagacttca tttccgctac agcagattcc attggtaaga atatgcagac
     7981 cttagctggt gtgagtgcaa acggattttc aacagagggt ttctatgttg gattcctctt
     8041 actcttgatt ttggttcttg gggtttatgt tgcctatacg ggactgataa agagagaaac
     8101 cacaaaggca attcatgcca ttatgaattt tgtgctggtg tttatcctat cggcttcctt
     8161 tattgcctac gctcccgact acattaaaaa aatcaatgac ttttcatcag acatcagtaa
     8221 tgccagttta tcacttggca cgaagattgt catgccccat tccgatagtc aaggcaagga
     8281 cagcgtggac ttaatcagag atagcctgtt ttccatacag gttcagcaac cgtggctact
     8341 gcttcaatac aacagttcag acattgaaag tatcggtatt gaccgtgtgg aaagcctgct
     8401 ctccaccagc ccagattcca acaatggcga agacagagaa aaaattgttg cggaagaaat
     8461 tgaagacaga agcaatacca atctaaccat tacaaagacc attaaccgtt taggtacagt
     8521 cttcttccta tttgtcttca atattgggat ttccatattt gtattcctat taacaggaat
     8581 catgattttc tcgcaggtac tttttatcat ctatgctatg tttctgcctg tgagctttat
     8641 tttaagcatg attccatcat ttgatggtat gtcaaaacga gccataacaa agctctttaa
     8701 taccattttg acacgagctg gaatcacatt gattattacg acagcattta gtatttcaac
     8761 catgctctat accttatcgg ctggttatcc gttctttttg attgcttttc tacagattgt
     8821 gacctttgca ggaatctact tcaagctggg cgatttaatg agtatgtttt ctctacagag
     8881 taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa cctcgtatgc ttatgcacgc
     8941 tcacatgcac cgtctacagc ggaaacttgg acgttccatg actactctag gggctgggtc
     9001 tgccattgtt acaggtaaaa aaggacagtc gggttcgggg agttctgcaa ggacacaagc
     9061 agatcactcc cgaccagacg gaaaggaaaa atcaacactt ggaaaacgta tcggtcaaac
     9121 catcggtaca gtagctgata ccaaagacag aatggtagac actgctagtg gtttgaaaga
     9181 acaggttaaa gatttgccga ccaatgcaag atatgcagta tatcaaggaa aatccaaagt
     9241 aaaagagaat gtccgtgatt taaccagtag tatttctcaa accaaagcgg acagagccag
     9301 tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt gcgaagcgtc gctctgaaat
     9361 ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt catgaaagac cgactacaag
     9421 acaagaacaa tatcatgatg aacagacctc aaaacagtct aatattcaga cttcatataa
     9481 ggaatctcaa caagccaaac aagagcgtcc agcagttaag tccgattttt caagtccaaa
     9541 agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt caaaagccag caacttcaac
     9601 cactacagca gatagaactt cacaacgtcc aatcacaaaa gaacgtccgt ctactgttca
     9661 aagagtacca ctacaaaata caagaagtag accaccaatc aaaaccgcca ccattaagaa
     9721 agtcggtaag aaaccatgaa gttgaaaact ttagtgattg gtggttctgg attattcttg
     9781 atggtcttct cactgcttct gtttgttgcc attttatttt cagatgaaca ggacagcgga
     9841 atttccaata ttcattatgg aggtgtgaat gtttccgcag aagtgctggc tcataagcct
     9901 atggtagaaa aatatgccaa agaatatggc gttgaagaat atgtcaacat acttcttgcg
     9961 attatacagg tggaatcggg cggtactgcg gaagatgtta tgcagtcctc ggaatccctc
    10021 ggtcttccac ctaattcatt gagtacagaa gaatccatta agcaaggtgt gaagtatttc
    10081 agtgaattat tagccagtag cgaaaggctc agtgtagatt tagaatcggt tatccagtcc
    10141 tacaattatg gtggtggttt cttagggtat gtggctaatc gtggaaataa atataccttt
    10201 gaactggctc aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc ttaccccaat
    10261 cccatagcca tacctatcaa tgggggctgg cgatacaact atggcaatat gttttatgtg
    10321 caactggtaa cgcagtatct tgtcacaaca gagtttgatg atgatacggt acaagccatc
    10381 atggacgaag cactgaaata tgagggctgg cgatacgttt acggtggagc ttccccgact
    10441 acttcttttg attgtagcgg actgacacaa tggacgtatg gaaaagctgg aattaactta
    10501 ccacgaaccg cacaacagca atatgatgtg acccagcata tcccactatc ggaagcacaa
    10561 gctggcgatt tggttttctt tcattctacc tataacgctg gctcttatat tactcatgtt
    10621 gggatatacc ttggcaataa ccgtatgttt catgcaggcg acccaatcgg ttatgccgac
    10681 ttaacaagcc cctactggca acagcattta gtgggagcag gacgaatcaa acaatgagaa
    10741 aggaagattt aatgatgaaa tttagaaaaa atcagaataa agaaaaacag ataccaaagg
    10801 aaaagaaacc tcgtgtctat aaggtcaatc ctcataaaaa ggttgtgatt gccttgtggg
    10861 tacttttagg gcttagtttc agctttgcga tattcaagca ctttacagct atagatactc
    10921 atactattca cgaaacaact atcatagaaa aggaatacgt tgatactcat catgtagaaa
    10981 attttgtaga gaactttgcg aaagtctact attcatggga gcaatccgat aagtccattg
    11041 ataatcgaat ggaaagtcta aaaggctatc tgacagatga acttcaagct ctcaatgttg
    11101 atacagtacg caaagatatt cctgtatcgt cttctgtaag aggatttcag atatggacgg
    11161 tagagccaac tggcgacaat gagtttaatg taacctacag tgtagaccag ctcattacag
    11221 agggagaaaa tacaaagacc gtccactctg cttatatagt gagtgtctat gtagatggtt
    11281 ctggaaatat ggtactggtt aagaatccga ccattaccaa catacctaag aaatcaagtt
    11341 ataaaccaaa agccattgaa agtgagggga cggttgattc cattacaacc aatgaaatca
    11401 atgagttttt aacgacgttc ttcaagctct atcctacagc gacagccagt gaactttcct
    11461 actatgtgaa tgacgggata ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg
    11521 taaatcctat tcacaatcgt aaggataatc aagtcacggt atcgctgaca gtggagtata
    11581 tcgaccagca gaccaaagca acgcaggtat ctcaatttga tttggtactt gaaaagaacg
    11641 ggagtaattg gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa
    11701 tcacgtactc tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta
    11761 ttatttaagt atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa
    11821 ctgtcaattt gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt
    11881 ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccgga gaatatctgt
    11941 atgctttgta tgcctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg
    12001 gatttttatg cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt
    12061 ggagttttag ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac
    12121 agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg
    12181 cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa
    12241 aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat
    12301 cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa
    12361 gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt
    12421 atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag
    12481 aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg
    12541 acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta
    12601 gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata
    12661 agatttcaga attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg
    12721 attgataacc ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg
    12781 tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca
    12841 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
    12901 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
    12961 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
    13021 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
    13081 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
    13141 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
    13201 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
    13261 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
    13321 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
    13381 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
    13441 ggatacttaa atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa
    13501 caaggattgt atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac
    13561 tatagccctg ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa
    13621 gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg
    13681 ccacaggaat atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta
    13741 gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt
    13801 caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag
    13861 ttaaaagggt accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt
    13921 cggatagata aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat
    13981 ataaatatgg tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag
    14041 tgtaaaggag gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa
    14101 taaaacacga ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc
    14161 gaaatgcaga caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga
    14221 gccagacgca aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct
    14281 ctttccgtta tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc
    14341 gctggctaaa gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc
    14401 tatctgtaat cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac
    14461 ggaaacatga agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt
    14521 attttcaata ttagttaagt accgtgggtc aatttcaatc aatgctccca cttgttcacg
    14581 agttaaacct cgtttcaatc gagcttcttt aatggctaaa ccaaaggctc taaaatcata
    14641 tttatcttct tttttacgca tagtagacca cctctataca ttttattgtt cctactgaat
    14701 taaaaacagg tatagaaaaa cgtgttatat ggtttatagg tttatattta ataaaaagca
    14761 ctactaaacg ccaataaaaa aaaccgttat atggtagtgc tatttacgct gttaaaatat
    14821 tgtatattac ttccaaatgg cggtttgttg gaggtcaacg tcgccatgaa gtacatcata
    14881 tacaataaat ttccttacat tgggttcttg tcaaaaaaag tcgtctatct gcaatagata
    14941 agtacgtcca ccaatgtggt tttataaatc atatagatag aataacagaa gcatgtaaac
    15001 agagaaataa atctgtttat atgctttttt ggctattcag aactttttta caaagtttat
    15061 ttatcagtaa tgcaacaaat ccccctttca cattgggact aagagtgaaa ggagataaac
    15121 gagcaaggct cacttccttt cctagacaga aagggggtga gaaacatgaa accatcttct
    15181 tttcagacca caatagaaaa tcagtttgac tatatctgta aacgtgctat ggaagacgag
    15241 cgaaagaatt atatgcttta tctttcaagg attgcaaagc gtgaggtgtc cttttcggat
    15301 gttggcgatt atcttgttag ccagtttgcg acaacagata actattcaac tgactttcag
    15361 atttttacac tcaatgggtt atcagtaggc gttgaaaatg atttgttgag tgaagcatta
    15421 cgtgagttgc cagacaagaa acgtgaaatt ctactgctgt tttactttat ggacatgagc
    15481 gattcagaaa ttgcagacct gttgaaattg aaccgttcta ctgtctatcg gcatagaacc
    15541 agtggactag ccttaattaa aaagtttatg gaggaatttg aagaatgaaa acacaatatc
    15601 ctatgattcc ctttcctctc attgtaaagg caacagatgg cgataccgaa gcgattaacc
    15661 agattctaca tcattacaga gggtacataa cgaagcgttc cctacgactt atgaaagatg
    15721 aatatggcaa tcaaagtatg gtcgttgatg aagtcttacg tggaagaatg gaaaccagac
    15781 tgattacaaa gattttgtca tttgaaatta agtaatatcc tctctccttt cgtggaagcg
    15841 tgctaaacca ttccacgctt cccgaacagg gaggtttgtt attccaccaa agcatattga
    15901 gctttcaatg tgttttgata ggctaacgag ccattgttct ttgaaaactg aataaaagta
    15961 atcgaatacg tttcgataag aaaagagcca acggaactaa ccgccatgac ctatcttata
    16021 aagatagcga gcgattcatg ttagtgatcc gagaagcaat ctttagcagg attgcctgca
    16081 acgacattct tatcgtgata atgatactcc catacagtca atagtccgag cgtgataaaa
    16141 ccgtcgcagg caatgagtat ggctacatga gaaccatgca ggggtggaac tcccgtgagc
    16201 tttgctaaag ctgttcgatt gctggtaaaa caacttttat gaaatccaaa taagtgattt
    16261 ggaaaggagg attttatgaa gcagactgac attcctattt gggaacgtta taccctaacc
    16321 attgaagaag cgtcaaaata ttttcgtatt ggcgaaaaca agctacgacg cttggcagag
    16381 gaaaataaaa atgcaaattg gctgattatg aatggcaatc gtattcagat taaacgaaaa
    16441 caatttgaaa aaattataga tacattggac gcaatctagc gtcgccaaag ggtcttgtat
    16501 atgataaaat agtattaagt cgtatcaagg ctctttccat aaaggaaagg agcaaatgcc
    16561 atgtcagaaa aaagacgtga caataaaggt cgaatcttaa agactggaga gagccaacga
    16621 aaagacggaa gatacttata caaatatata gattcatttg gagaaccgca atttgtttac
    16681 tcgtggaaac ttgtggctac agaccgagta ccagcaggaa agcgtgattg tatctcactt
    16741 agagagaaaa tcgcagagtt acagaaagac attcatgatg gtattgatgt tgtaggaaag
    16801 aaaatgacac tctgccagct ttacgcaaaa cagaacgctc aaagaccaaa ggttagaaaa
    16861 aacactgaaa ctggacgcaa atatcttatg gatattttga agaaagacaa gttaggtgta
    16921 agaagtattg acagtattaa gccatcagac gctaaagaat gggctattag aatgagtgaa
    16981 aatggttatg cttatcaaac catcaataac tacaaacgtt ctttaaaggc ttcattctat
    17041 attgctatac aagatgattg tgttcggaag aatccatttg actttcaact gaaagcagtt
    17101 cttgatgatg atactgtccc taagaccgta ctaacagaag aacaggaaga aaaactgtta
    17161 gcctttgcaa aagctgataa aacctacagc aaaaattatg atgaaattct gatactctta
    17221 aaaacaggtc ttcgtatttc agagtttggt ggtttgacac ttccagattt agattttgag
    17281 aatcgtcttg tcaatataga ccatcagcta ttgagagata ctgaaattgg gtactacatt
    17341 gaaacaccaa agaccaaaag tggcgaacgt caagttccta tggttgaaga agcctatcaa
    17401 gcatttaagc gagtgttagc gaatcgaaag aatgataagc gtgttgagat tgatggatat
    17461 agtgatttcc tctttcttaa tagaaagaac tatccaaaag tggcaagtga ttacaacggc
    17521 atgatgaaag gtcttgttaa gaaatacaat aagtataacg aggataaatt gccacacatc
    17581 actccacata gtttgcgaca tacattctgt accaactatg caaatgcagg aatgaatcca
    17641 aaggcattac agtacattat gggacatgct aatatagcca tgacgctgaa ctattacgca
    17701 catgcaacat tcgattctgc aatggcagaa atgaaacgct tgaataaaga gaagcaacag
    17761 gagcgtcttg ttgcttagta gtacaaatga atttactact tatttaccac ttctgacagc
    17821 taagacatga ggaaatatgc aaagaaacgt gaagtatctt cctacagtaa aaatactcga
    17881 aagcacatag aataaggctt tacgagcatt taagaaaata taaaaagata attagaaatt
    17941 tatactttgt tttatagaaa atttatttaa aaagatatta acaggtttaa tatcaagtac
    18001 aaaataatat ttttgtttta gatagttttt tatcttatag tactattttt aaaaattcaa
    18061 tattttaaaa atagtactat aattatatat attaaggtat tttgatgatt tcagaaggaa
    18121 attcctaaat tatgaataaa cactatttcg aattattaat attttctttg ggaattgttt
    18181 ctagattatc agtctctctt aaaaaatacc aaatcaatat aaaatttggt catt
//
LOCUS       DATRSD010000043        17633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRSD010000043 DATRSD010000000
VERSION     DATRSD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.043"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            22..261
                     /locus_tag="VBD85_001189"
     CDS             22..261
                     /locus_tag="VBD85_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7133747.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            361..894
                     /locus_tag="VBD85_001190"
                     /pseudo
     CDS             361..894
                     /locus_tag="VBD85_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
     gene            complement(970..1464)
                     /gene="tpx"
                     /locus_tag="VBD85_001191"
     CDS             complement(970..1464)
                     /gene="tpx"
                     /locus_tag="VBD85_001191"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO7133748.1"
                     /translation="MTTFLGNSVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAEGLDNVIL
                     LSDFYDHSFGQDYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(1537..2196)
                     /locus_tag="VBD85_001192"
     CDS             complement(1537..2196)
                     /locus_tag="VBD85_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO7133749.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(2196..2960)
                     /locus_tag="VBD85_001193"
     CDS             complement(2196..2960)
                     /locus_tag="VBD85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7133750.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQSDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRHQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLERADQMLAEL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            complement(2965..3606)
                     /locus_tag="VBD85_001194"
     CDS             complement(2965..3606)
                     /locus_tag="VBD85_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7133751.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(3770..4321)
                     /locus_tag="VBD85_001195"
     CDS             complement(3770..4321)
                     /locus_tag="VBD85_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7133752.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKTIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(4343..4540)
                     /locus_tag="VBD85_001196"
     CDS             complement(4343..4540)
                     /locus_tag="VBD85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7133753.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(4599..5141)
                     /locus_tag="VBD85_001197"
     CDS             complement(4599..5141)
                     /locus_tag="VBD85_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7133754.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(5197..5391)
                     /locus_tag="VBD85_001198"
     CDS             complement(5197..5391)
                     /locus_tag="VBD85_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO7133755.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(5401..5982)
                     /gene="amaP"
                     /locus_tag="VBD85_001199"
     CDS             complement(5401..5982)
                     /gene="amaP"
                     /locus_tag="VBD85_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO7133756.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSLSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(5997..6245)
                     /locus_tag="VBD85_001200"
     CDS             complement(5997..6245)
                     /locus_tag="VBD85_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7133757.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(6293..6538)
                     /locus_tag="VBD85_001201"
     CDS             complement(6293..6538)
                     /locus_tag="VBD85_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7133758.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            complement(6950..9229)
                     /gene="pcrA"
                     /locus_tag="VBD85_001202"
     CDS             complement(6950..9229)
                     /gene="pcrA"
                     /locus_tag="VBD85_001202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO7133759.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDASSNVMMSPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(9335..9721)
                     /locus_tag="VBD85_001203"
     CDS             complement(9335..9721)
                     /locus_tag="VBD85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO7133760.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKGEMVTTAIFTMFVTGERKEK"
     gene            complement(9854..11179)
                     /locus_tag="VBD85_001204"
     CDS             complement(9854..11179)
                     /locus_tag="VBD85_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO7133761.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     regulatory      11487..11619
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11776..13122
                     /locus_tag="VBD85_001205"
     CDS             11776..13122
                     /locus_tag="VBD85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO7133762.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFIQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLFGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLVLSPIIVKETQKYFSETKNITKP"
     gene            13186..14421
                     /locus_tag="VBD85_001206"
     CDS             13186..14421
                     /locus_tag="VBD85_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7133763.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVYFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITERVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            14580..14972
                     /locus_tag="VBD85_001207"
     CDS             14580..14972
                     /locus_tag="VBD85_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7133764.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKY
                     KIIRLRHIDAVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            14953..15648
                     /locus_tag="VBD85_001208"
     CDS             14953..15648
                     /locus_tag="VBD85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO7133765.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLRIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            15717..16340
                     /locus_tag="VBD85_001209"
     CDS             15717..16340
                     /locus_tag="VBD85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO7133766.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(16742..16897)
                     /locus_tag="VBD85_001210"
     CDS             complement(16742..16897)
                     /locus_tag="VBD85_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133767.1"
                     /translation="MAFVKKYIAIEIENTKEFEELYDDYLKKASELREAVQKLEQFQF
                     TGILNNN"
     gene            complement(17490..>17633)
                     /locus_tag="VBD85_001211"
     CDS             complement(17490..>17633)
                     /locus_tag="VBD85_001211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133768.1"
                     /translation="NIKKMLFGSYDYHKISEDGKEKLSIKIDSGWVIGLSILGLIGWL
                     VLK"
BASE COUNT         5774 a         3354 c         2631 g         5874 t
ORIGIN      
        1 tacttaaaat gtcatctata tatgacactc ttcttaaatt ctttactaaa ggagctaata
       61 atcgtgaatc gtcttagaga atacagaatt aaagctagca tatctcaatt agctttagca
      121 aaatcagttg gtgtcgccag acaaacaata aaccttattg aaaataataa atacaatcct
      181 tcgctagatc tttgtataag actagcggaa tgcctacaaa cagacctcaa tactcttttt
      241 tgggatagta gacatctata acagattaga ctctctaaat ctatgaaaac tcgggaggtt
      301 aggtcgtgtt attatttcaa tctcaaactt tattaaatag ttcaaggata ggagaatatt
      361 atgaaagaaa agaaaaattt ggggataaaa ttaataaaat ccctatatgg tattaatggc
      421 ccattcgatg aatacagaga acaaaaaatc aattaccttg gaaatctatt atttgcaacc
      481 actagtctct atcacatttt atcctctatc atcgcctagt tcacggtaag taaatatccc
      541 acagagactg catatactct cctagcttta acattctttc tatatttttg tatttttcta
      601 attgttattc cctcttatct caacagtcat gatttaataa cgccactaga agtctcaaaa
      661 aatgactatc cagtagcaat aaagaaaaac aaacaaaaat ttatcaaaat cgggatattt
      721 tatactgtca tccagtggct tatcttgggt ttaacaacta acaataacac tccgctgatt
      781 gtatcactat tttctttaaa agttattttg tctagtatta tgtccggtgt agcttacaat
      841 ggagttatgt atctaatatt taaaaacaat gttgatattg aaaaagatga ataatgacaa
      901 catttcattt ctaattgttc tcactgtcta tataaataat aaggctggaa aattttccag
      961 ccttattatt tatggtaata tctttgcagc attaatcgcc gcttcgtagt caacatctga
     1021 attaacattg tcaacgtatt cggtgtaagt caccttatta tgctcatcca aaataagaac
     1081 tgcacgcgtt aacaaatgcc actcattcat caaaagagcg taatcttgac caaacgaatg
     1141 atcataaaaa tctgataata aaatcacgtt atcaaggcct tcagcgctgc accaacgctt
     1201 ctgagcaaat ggaagatcca tagaaacagt aataaccacc gtgttatcta gctctgataa
     1261 ttcctcatta aaagtacgtg tttgttttga acaaatacct gtatcaatag aaggaacaac
     1321 gctaataact ttctttttcc cttcaaaatc ttttaaagat ttttgagata agtctgtagc
     1381 tattagtaaa aaatcttttg caatatcccc aacttgtagc tgttttcctg taaaagtaac
     1441 agaatttcca agaaatgtcg tcataagttt acctccttat caattacctt tattttacta
     1501 tgaagcttct tctttttcga ataattgaac tgataattaa aggtacttac ttccttcgcg
     1561 tatgctcaaa gtcgacaact catcataatg attactgata aagtccttta cccatacttt
     1621 attatacttt gaataatccc taagcgccca gccaatcgcc ttattgataa aaaattcttg
     1681 acttcctgta tttcttaaaa taaaaagtga caaaatatca gtatttgttt tttctttaaa
     1741 tcctagttga tgctcaatag ctagtcgcct catccaaaaa tcttgatcaa gtgaccattc
     1801 tgacagtaaa ttcataacct tatcatcttt taaggataag ttcccaaaaa tcttagtcag
     1861 aacatcaata ctatcccacc actgttgatg acgagcgtaa aactctagtc tagagcaatc
     1921 attataggtg agaaattgac tcatagctaa tagataatca agtacaaaat gatgatattc
     1981 acgatagtca ctttgccaac atttatcaag caattgccaa tcaatttttg cttgtctttt
     2041 agcggccttt ataagctctt tgtagagcat acgtcgctca ggtgatttaa taccatagca
     2101 cttaaaatga tggcgtaaat aagcctcctg ttgcttagaa acttctttgt cacttgcagc
     2161 tttaaatttc ctctctaaac ttaggaataa atccattacg taatcccctt gtatttttca
     2221 aaaagcattg taattccttc atgtacagat tctcttgata cacgtaaatt agcgtaagga
     2281 gtcagttcat caaaatcccc tacttgaaca ctataatgcg cagcatcaaa catagaaata
     2341 tcatttttat cattgccaaa agctataaaa cgattcccaa ataatttctt agtggctttt
     2401 cccttgtcaa ctgcaatggg atttatgtac aaacattttt caaattcgtg gaaaaagtga
     2461 ctactaagtc caaaacgatt taattccgct aacatttggt ctgccctttc cagctgatca
     2521 ccaaaataca acaccatttt tatcggtttc tcaattttag aaacttctag aagctctccc
     2581 cttttttgag gatcaacata agcaataaag ggaattttag aagcctgata agtagcgtaa
     2641 ttaaagacct catcaacaaa atatggaatc tgatggcgat gacaatatga aataatagta
     2701 ctaaaaaagt cactttgtaa atagtaacta tccactaagt gaccattttc atgtaaagtc
     2761 gcaccattta aaccaataat ttttgataaa gatagcttgt cgccaaggat acctatagtg
     2821 tctctataag atcgcgccgt agcaaaagtg actctatgac cataatcact ctgggctctc
     2881 tctagtattc cttgtatttc tttagataga ctcataccat caaaacaaag agttccatct
     2941 atatcaaaga caaaatccat tctctcaaag ccccatcctt aagttaaaat aggttttcat
     3001 acgtgcataa acatcttcat caccaacaaa aaagatatga tctccttgct ctataactga
     3061 gaaaggcccc ggagaaacaa taaatttccc ttcgtgttca atagccacaa tagtcgcacc
     3121 agtttgatgc catacattta attctcctat tgacttacca aggtgttcag aatcttcact
     3181 gacaataatt tcatatggtg ctagtgggta ttgtttacta accgcacgtg tttgaagcaa
     3241 aaaatcatca actaaagtag ctaactcttc catttcttgt tgctgagcct taatattatc
     3301 acggatttta cctttcaaaa tagcaactga atgagaggtt tcatattggt ttaagaatgc
     3361 aattgctttt tctttagaaa gaataatggc tccactgcca tgctttaaag ttaaaatctg
     3421 taaatctgct aaaatattaa gacccttacg tgctgtttct ggtgatacgt tgaatgttga
     3481 agcaatagtt gttctagatt tcaacttctc tccaacttca taatctccat ttgcgattct
     3541 ttgtgcaacc gctactgcta ttttttgata tttagatgtt acaatttcag attgttcaga
     3601 aaccatccaa ccctccttat cctaataaaa acatacatat taaaccacaa ttagcaaaat
     3661 attgcaaata tatctttttt gttaatatct aatgtcttta gtaaatatca tataaaagga
     3721 gctggtctcc cagctccgag cgtcttatca taaaaagtag tttactgaat taagattcac
     3781 tagtacgagc ttctgagatt ttttcttttg cagcctcagt accatttgat actgcttctt
     3841 ttgttttctc tgctccagag gaaattgctg ctttagcttt gccagcttgt tcagaagcaa
     3901 agtttcctgt tgcttgtgct gcatcagata cacgatcttg tactgtcact gagtcagctt
     3961 catgttcagc tttagtctta acatctacga catttgcgtt caattcaaca actttaaggt
     4021 gggtcatcac ttcaacgttt tgactgacaa tcgttttaat gctttcaacg atagctggga
     4081 tatccttgcc gtattcaaca acgatgtcta aatcaactgc aacttcttta gtaccaactt
     4141 caacatttac accatcagtt actgagtcag aatttacaac gctatttttt agattagaga
     4201 agaaacctcc actcacagcc aagagtccgt ctactgattc taaagcatgt ccaacaattt
     4261 tagcaattac cttttcttca taagttactg aagttttaat atcttgtttt tgattttcca
     4321 taaataaatg acctcaaacc ttctagtcct ttttcttaaa gctctctgaa gcacccttaa
     4381 tggtatcttt aatgtcagca agtttttctt ttgcatgacc tttcaattta tcgactttac
     4441 cttcagattc aacttcttta tcgccagtaa gtttcccaac agattctttt actgaaccag
     4501 aaaccttatc aacttttgca tcaaattttt cttcagacat aatagcctcc tactaataac
     4561 aatactttaa acaatacttt aaactatact taaatcaatt attgtacacg tggttcagac
     4621 ttcatgtctt ccactttatc aacaccgtta cctacggcac ttttaacgtt tgaaacttga
     4681 ttcgaagcaa attcacctgt tgcttgtgct gcacttgtta cacgatcttg aagagttact
     4741 gaatcttctt catgttgagc acgagtttta atatcaacaa cattaacatt aacttcaacc
     4801 acttcaaggt ctgtcatacg tttcacttca gcttcaacaa cttttttgat atcggcaaag
     4861 atagttggaa catgtttttg gtattcagca acaatatcca aatcaacagc aacttgcttt
     4921 ttacccactt caacatttac gccatctgta actgaatcag aatttacaac gctatttttt
     4981 aaatttgaga aaaaaccacc atttacagcc aaaagaccat ctacatgctc aatcgcaatt
     5041 ccaacaattt tttcaatcac tttatcttcg aaagttaatt ctccacgaac agctgtcgtg
     5101 ccagaattgt tagttgtgtt tttgatgtaa gtttcagtca taatagttcc tctttctttt
     5161 atttaaataa tgtagaataa atataaacga aaaattttat tttctcttat taaagaattg
     5221 gtcaagcata cctgttcttt gcacataaag cccagcataa gcacctagca caatgataac
     5281 aagagcaaga attgttttaa agaatccaat ggttacaatc attgcagcta aaacgagccc
     5341 aataactgca cctcctagtg gatatctgta tttcctcacg aattctgaca tattagactc
     5401 ctattctaca cgacttttat tcgtcttagc atttccaaca ttttcttctt caacttgctt
     5461 aacaaatact ctagtgttca ttttcttttc caacccaaag aattctttta agccaagttc
     5521 aacatcactt tgaatatgtt caagttgttc cggtacattt gtacgagaag ttaaaagacc
     5581 tttaacatca actttcactt ttttcttata taaatgagct gtcacactaa gagatttcat
     5641 caaaccagtt tttaatactt ctgttttaac aaatccctct attgcagatt tttttaacat
     5701 caatttacca gttttatcag ccagtttata ctctgtgtat cgtcttggat aaaacagaac
     5761 taccaatata gccagtaaaa cgattacaaa cagcactaat gctccccaaa agaaataatg
     5821 atacatacca acgtcaatcg gtgatggcaa attaaaatcc caatctaacc aattaaaaga
     5881 tggtagataa atgtgttgtt tactgataac agctacaaat cctagtaggg taagggagat
     5941 tagacctagc aaagtatata aactttttag tccttttgac ataggtaact ccttttctag
     6001 ttattcattt tacctagtac aaatgaagta acaatgacaa caataatcgc acctacgatt
     6061 gaaggaatca aagccatacc tgctaattta ggcccccatg ttcctagtaa actttgacca
     6121 acaaatgaac caactaaacc agctaagatg ttagctatcc aacccattga accacctttg
     6181 ttagtaacag cccctgcaat agcaccgata atagcaccta cgattaatga ccaaattaaa
     6241 ctcatgataa ataccttctc tttcttgact aatcattcta ctagttatgt agttatgatt
     6301 ttttgcctaa tacaaatgaa gtaacaatga taacgataat cgcacctacg attgaaggaa
     6361 tcaaagccat atctgctaac ttaggccccc atgttcctag taaactttga ccaacaaatg
     6421 aaccaactaa accagctaag atgttagcta tccaacccat tgaaccacct ttgttagtga
     6481 ttgctcctgc aatagcacca ataatagcac caacgattaa tgaccaaatt aaccccatga
     6541 taaatacctt tcttcctaag tacatctcag tgtacaaact tattttgttc ttaactaaat
     6601 aacaagagtt tcttaagtga ccactcggca attaattaag ttgttaacta tactaatatt
     6661 atacaacttt ataaagtgac tgccaagtaa aaagcttggt aaatgctttt atttataagt
     6721 gaccacttga tatttaattg atattgttat tatactcttt ttagaaaata atgcaagaga
     6781 aacgcttctt tctttaaaat agtatggtca cttttcaaaa acgctttaat atcaaccttt
     6841 ttagtaagta aaaaccaaat gataacttat tttgtctatt caaatgatgc aaaaagacta
     6901 agaatacaat tcttagtctc tgattattta agcatataat agaagaaagt tagttttcct
     6961 ttttactaat aggagcgaca ctcgctaata atttctttaa cccaatacct ggaaaattta
     7021 tttttaactc ttgagtcttt ccgctacctg aaacttcaag caccgtaccg tccccccatt
     7081 ttttatgagt tgcaacatca ccaatctccc atgatgtctc atggctattg ttcgcgttaa
     7141 gagcctggag ttgggcagtt acttgaggtt gtgaattact tttacgtgcc tgaagcgctt
     7201 gttgaagact cattccttga ccaaattgtg taggctgttc ttttgaatat ttcacaccaa
     7261 atgaagaatt aactggtcga gccaaccctt gatgttgtat cagctcatca tcaatctcac
     7321 gtataaaacg ggttggtcta ttataactag ttttaccaaa taaagtacgg gtattagcat
     7381 tggtaaggaa aagtatttgt tctgctctcg taatcccgac ataagctaaa cgacgctctt
     7441 cttctaattc atcagcatcc tctattgcac gcgataaagg aaatactcct tcttccatac
     7501 cgattaagaa aacaactgga aattctagac ccttggcagc atgtaatgtc attaaagtga
     7561 cctcagccgt ttcagtggcg ctatcatcag tatctgctat taaagctaaa tcatttaaaa
     7621 aacgcgatag acgatctaaa ccagtttcac cctctactgt aatctcaggg ttatcgtcaa
     7681 aatttttagt cactgacaag aattcttcaa tattttcaat acgcgcttga ctttctaatg
     7741 tattttgaac ttgaagtgct tctagatatc ctgtcttatc cagtaggttt tctgtaatct
     7801 ccgttacagt taaactatct agcttactac gtagagtcaa aatcagatta gctaaatccc
     7861 aaacagcttg agcagccttc ccttttaagg gcgacatcat gacatttgaa gaggcatcaa
     7921 gaagagacat gttctgttca taggcaaatg accgtatttt ttctaaagtg cctggcccaa
     7981 cccctctttt aggttcattt acaatccgct caaaagaaat attatcagaa gtatttgcaa
     8041 ggatattgag ataagcaata acatctcgaa tttctttacg actatagaat tttgttccac
     8101 caaccattgt atatggaata ttggatttta aaagtgcttc ctcaatagta cgagattgcg
     8161 cattcgtacg ataaagaacg gcaaaatctt tgaaattttt tccttgttct cgaacgatgt
     8221 tatcaatagt tgatgctaca aaaacggctt cttcttgctc attgttagct ctatgatata
     8281 caatttgctc accttcatca ttttgagtcc ataatttttt gggacggcga tttttattat
     8341 gattaatcac attattagca gcttgaagta ttttcttagt cgatcgataa ttttcttcta
     8401 ataatacaac tttggcttgc ggatagtcct tttcaaaatc aagaatattt tgcatatcag
     8461 ctccacgcca tccgtaaatg gattgatcgg catcaccaac aacacaaata tttttgaaac
     8521 gcgaagctaa taacttaact aattggtatt gagcgtggtt agtatcttga tactcgtcta
     8581 catggatata ttgatacctc tgttggtaat aggccaaaac atctttattc tgatcaaata
     8641 atcgaagtgt catcataatc aagtcatcga aatccatggc ctcacttcta cgtaattcct
     8701 cttgataagc tttatagcat ttagcaatta cctgtgtata catatctccg gcttgcttct
     8761 catatgcaat ttcatcaaga aggtcatttt tagcattaga gattgtacct aaaattgaac
     8821 gttcattcca cttttttgtg tccaaattta attgcttaat gattcgcttc attaaagtac
     8881 gctgctcacc tggatcaacg attgtaaagt tacgattata tccaatataa tcagcttcac
     8941 gacgtaaaat acgaacacac atactatgaa aagttgcaat caaagtatct tgggtagctg
     9001 gattcaaggc gatagcacgt tcacgcattt cacgcgctgc tttattagta aaagtaatcg
     9061 ctaaaatatt ccaaggatta acatattttt catctattaa ataagcaata cgatgagtca
     9121 gaacacgtgt ttttccagag ccagctcctg ccataatcaa aagcggtccg tctgtcgttt
     9181 gtaccgcttc tgcttgttta tcattcattc caattattaa aggattcatt tctactccta
     9241 attttgtcaa aattgcctct ctagtatatc attttttcat ctttttccca atagaaaaag
     9301 gagttaaaca gacgctgtct aactcctacc ttttttactt ctcttttctc tctccagtta
     9361 caaacattgt aaaaatagca gttgtaacca tttcgccttt ctgattttta atgtgaacat
     9421 caacgacttt tgtagtacga ccattatgaa cacaagtacc gatgacagta agcagatcat
     9481 ctaatcggcc tgccctcaaa tagttgatat ttgattgtag agttaccgct tcatatcctg
     9541 ttgaaatagc aactaaacca ctgacttgat cacacagtgt aaacaaataa ccaccatgag
     9601 cattaccata atagttgagc gaagagtcaa cgaccttcgt tgttaaggta acttgacctt
     9661 cttcccagtt ctcaatttcg taattctcaa atacttttac ttgttctaaa ttgaatttca
     9721 ttctactata acctcacttt ttctatgacc attatagcag aaaataagga aaccttaact
     9781 gtaaacgttt ttattttaaa ctttttaact gatctatcat aacaaaagag ctcagatact
     9841 ctgagctctt ttgttatgat ttcatgcttt cttttggcaa tattaaattt aatataatac
     9901 ccactatagc acttaatgct gttcctgata aagttagagc acctagatca aggactgctc
     9961 caccgaggcc taaaactaac atggaactag caataatcag attacgaacc tcagcaaaat
    10021 tgacacgatt ttcaatcaac acttttaatc cattacttgc aataacacca taaagtaata
    10081 tagccattcc accaagtact gcactaggaa ttgtagagat caaagctgta aatttaccaa
    10141 agaatgaaaa agcgattgca ataaaagctg cattacgaat aaccgttaca gaagctatac
    10201 gcgtcatgcc aataacacct gtattttcac cataagtagt ttcagctggc cctcctataa
    10261 gagcagaaaa tgcggttgct aaaccatctc caattaaaag acgatttaaa cctggttttt
    10321 ttagaaagtt acgcccacaa atctgaccta aaactgtatg atcaccgata ttttcagcaa
    10381 tcgttacaat tgagattgga agaattgcaa gcatttctgg tccaaagtaa agtttataac
    10441 tgtgaaataa acctgtctta aacggtaaat agaattttgg caattcaaac caggcagctt
    10501 tttcaactgg agataaatct accaagccaa gaatgattga taaaatataa cctccaataa
    10561 ttgcaaataa aaatggaata atcttgataa aacctttccc tttagtattg ataaaagctg
    10621 caatcaagaa tgtaacaaca gcaactagca ttttacgcca atcaccttta gcgacaaaac
    10681 ctgcattagt tacagcagag tttgccaaac ctaaaccaat aacaataatc ataggtccaa
    10741 tgattattgg tggcaaaatc ttatcaaccc atgaatttcc tacgaactta ataaccgtcg
    10801 ccacaacgac atagattaga cctacaaaaa gaatacctgt ttgagcagcc gaaatatcgc
    10861 catgcatttg tttcatggct aatgccatag ctgtaatata agcaaatgat gagcctaggt
    10921 aaacagggac tttaaatttt gtcgcaactt gataaatgag cgttccgcaa ccagaagcaa
    10981 ataaagccac tgatacaggc atccctaaaa taagcggaac taatatcgta gctccaaaca
    11041 tggcaaaaac atgttgaaaa cttaacaaca aaccttgaaa taattctggt ttttcatcga
    11101 tatctagtaa taagttagta gaatgtactt ccttatctac tagaatttga ttactttcac
    11161 ctttagcatt taaagtcata tctaaactag tcctcttggg catacaaaaa agcccacttt
    11221 ctatagcaat atagtcatag gctttatttt tttcctgatg tttttctcac ctcacgggat
    11281 gagtttaaaa acctacgctg aaagaattat agcataagac attttagaaa acaagacttt
    11341 tttgaaacta tattgacaaa actatgcttt ttcattatga tagttaacat aataaaatat
    11401 accgaatgac gtcattcagg agaagaaagt tagactttcg ccgaaggaat tacactctca
    11461 ggtgtcttaa gacaggactg attgacagac ggacttctgg agagacctat aagtagcaac
    11521 atctttgtat tgacaccaag atgtgctcta ggcgccgaag gggcaagaag agtaaaacaa
    11581 ctcctccaat ctctcaggca aaaggacaga agctaaaagc caatattaat aatgagtagt
    11641 aagcttatta agtttactac tacctttatt tgtgcgcttt ttagctagca tctttcagaa
    11701 gttatctctt ttagagataa cttttttcgt ttcattacag aatccatagg tatgtcatgt
    11761 atcaaaggag aacatatgct aacacttttt actcatatca atagcttcgt ttggggtcca
    11821 cctttacttg ctttattagt cggaacaggt atttacctat catttcgctt aggttttatt
    11881 caattgagac aactttctag agctttcaaa ttgattttcc gagaggataa cgggcaagga
    11941 gatatttcaa gttatgctgc ccttgcaact gctcttgctg caacggtagg gacaggtaat
    12001 atcgttggtg tggctacggc tattaaatct ggaggaccag gagctttgtt ttggatgtgg
    12061 gtagctgcct tttttggaat ggcaacaaaa tacgcagaag gattattagc tatcaaatat
    12121 cgcacaaaag atactaatgg agaaatttct ggaggaccaa tgtactacat tgtcaatgga
    12181 atgggacaaa aatggaaacc attagcagtc tttttttctg cagcaggtat cttagtagct
    12241 cttttaggaa tagggacatt cactcaagtc aatgcaattg cttcttctct tgagcatact
    12301 ttcaaaatat caactagatt taccagcctc attcttgctg ttatagtatt atttattata
    12361 tttggaggta taaaatctat ttcaaaagtt tcagaaaaaa tagtgccttt tatggctata
    12421 agctatattt tagcaactct gattattatt gcagttaatt acaataagat tccacatact
    12481 ttccaactca tctttagcgg tgctttttct gggacagcag ccattggtgg tttttctgga
    12541 gctatcgtta aagaagcaat tcaaaaggga attgctagag gagttttctc aaatgaatct
    12601 ggattgggtt ctgctccaat agctgcagca gcagctaaaa caaaagaacc tgttgaacaa
    12661 gggcttattt caatgacagg aaccttcatt gacactattg ttatttgtac tttgacaggg
    12721 attgcaattt tagttacagg aaaatggcta gaatttgatt tacagggtgc tccattaaca
    12781 caagcatctt ttaataccgt atttggctca ctaggtagtt ttgctctaac cttctgcctt
    12841 gtcttatttg cttttactac tattttagga tggagctatt acggagagcg ttgctttgaa
    12901 tatctatttg gcactaaatt tattaacgct tatcgtatta tttttgtcat tatggttggc
    12961 ttaggtggtt tcttacagct cgatcttatt tgggttatcg ctgatatcgt taatggttta
    13021 atggctttac caaatctaat agctctccta gttttgtcac caattattgt taaagaaaca
    13081 cagaaatatt tttcagagac taaaaatatc actaagcctt gaacttacta gttttatagt
    13141 ctgctcctaa gtgatataat gtgtaaatga aaggaaatta ggaaaatggc aacttctact
    13201 gaaaacttac aacttgcaaa aagagggcct attattagta tcattgccta tataacactg
    13261 gcagtcgcta aattagctgc aggttattgg tttgatgcta cttcacttgt agcagatgga
    13321 tttaataact tatctgatat cttaggaaat gtagcactct taattggttt acacttggct
    13381 agtcaaccag ccgactcaaa ccatcgtttt ggtcactgga aaattgaaga tttagcaagc
    13441 ctgatcacct cttttattat gtttgtcgta ggtattcaag tttttattca aactgtcact
    13501 aagattatta ataatactga tactaacatt gatcctctag gtgctatagt aggtgctata
    13561 tctgctcttg ttatgcttgg cgtttatttc tataataaac aattatctca aagagtaaag
    13621 tctagcgctc tggttgcagc ttctaaagat aacctttctg atgctgtgac atctatcggc
    13681 acatctattg ctatcattgc ggcttctcta aattttccta ttattgatcg tttagctgct
    13741 atcattatta cttattttat tcttaaaaca gcttatgata tttttataga atctgctttc
    13801 agcctatcgg atggtttcga tgattatcaa ttaaaacaat acgaaaaagc tatattaaca
    13861 attcctaaaa tatccgctgt aaaatctcaa cgtggacgta cttacggtag taatatttac
    13921 cttgatattg ttctcgaaat gaaccctgat ctctcagtct ttgaaagtca tgccattacc
    13981 gaaagagtgg aaaaactact cagtgataaa ttttctgtat atgacataga tattcatgta
    14041 gaacccgctt ctatcccaga agacgaaatt ttcgataatg tttaccaaaa attatacaaa
    14101 aacgaaaaaa tcatcctagc aaaaattccc ggttatgaga cctttattag ccctgacttt
    14161 tatatgatta atgaaaaggg aaacattatc acttctgaca tgctcactaa tgctacaaat
    14221 cattcattgg caagcaactt taaatatttc aatgtaaaat caattagtca aaaaacaaaa
    14281 cttgtgtctt atgaattgga aggtaaacga catactagta tttggcgccg taatgaaaaa
    14341 tggttcttaa tctaccatca aataactgcc aagtcaagtc cgtataaaac gagacgatat
    14401 caaatcactt cccttgatta atatactatt taatcagtta ctcttgataa aaatagctta
    14461 caaaaataaa ttaaaaagat tgagaaaaat ctcggtcttt ttaatttgtt tttatgtaaa
    14521 cgtttcatag ccttgaaaat tactttcaga gtagtagact ataatggaag gagtttttca
    14581 tgaaattata cgttcaatta atggttattt taaccttttc attcgctggt gaggttctat
    14641 ctactatatt caacttacct gtaccaggta gtattattgg attaatatta ctattcctag
    14701 cgttaaaata caaaatcatc aggctcaggc atattgatgc cgtggggaat tttctattag
    14761 caaatatgac cattttattt ttaccaccag cagttggact gatggagcac tttcaagaca
    14821 taaaacccta tctttttggg atagctatca ttatcttagg agcgctattt ctcaatattc
    14881 taactattgg actggtatcc caatggatca aaaagagata tgaaggagat tatccagaaa
    14941 ttggaggtaa aaatggcaac tttaacgaat aaccctatct ttggtattat gttaaccgta
    15001 tgggcgtatt acattggcat aaggattttt agaaaatatc cttcccctgc aacaactcca
    15061 cttcttttgg cgactatttt attaattgct tttttaaaat taacacatat ctcctataaa
    15121 gattactaca atggcggttc atttctaacg atgttaatta cgccttcaac ggttgtattg
    15181 gctattcctc tctatagaac ctttcattta atgaagcatc acattaaaag tattagcatt
    15241 agcatcatct tagcttctgt tatcaacaca gtttttaccg caatagttgc taagtttttt
    15301 ggtatgaaat atttcttagc aatctcactt ttcccaaagt ccgtaactac agctatggcc
    15361 gttggaatta caagtaaagc aggtggatta gctactatca ctctggtcgt agttgttatt
    15421 acaggcatct taacaagcgt tttgggtcct atttttctaa aattattaag gatagaagat
    15481 cctgttgcta tagggctcgc gctaggagga actggccatg ctataggaac tggacaagcc
    15541 ttaaaatatg gccaagttca aggcgccatg gctggactag caattggtat cacaggaatt
    15601 tgctatgtta ttgtcagtcc tttagttgca ggcttaatat tgaaatgagg aaaagaaaag
    15661 taaacccaaa ataattatgt tataatattt aaaaatagaa tttaggataa ataattatga
    15721 taccgttaaa aaaaataact gttggaacga ttgtctgtct ctcatttttg ggtttaacag
    15781 catgctcttc ttcaaatact cagcaaacct ctacttcaaa atcaaatgta tcacaacata
    15841 aaaatatcaa agctgaccat gaagaattac gtttgaaatt taataaagta aagttaggag
    15901 ttaaagcaaa taactttaaa ggaggcacta gtctcgctga attaaaacaa ttatttggtg
    15961 gcgaaccaaa tgaaaaattt gatacaccag cgggaaatgt cactcttaaa ggctatcgtt
    16021 ggaatgtcga tgatatctcc attactatac aattattaaa cgatagtagc attgttcgtt
    16081 caattagtaa ttttaagttt attcgtgatg ccaacatcac aacaaaagat tataatagtt
    16141 taaaaaatgg catgtcatac aataaagtaa aagaacttct aggagaacct gatgatattt
    16201 ctcaagcagt ttcttcagat aaggaggaac tacaggctgc atggattagt ggcatccagt
    16261 cgtcagacag tgatccggga attaacctta catttgaaaa tgataaactc actaacaaac
    16321 aacagcatgg tttaaaataa aaaggcatta acgccttttt attaatacct taattactta
    16381 ttagaaatat tttattacac aatagtttaa tctcagtacc ttaacttagt cctttaatgg
    16441 tcatactaac taattatgat actaacttac acagatttca aactaattct tccttatttg
    16501 tcaaccttgg ttttacttat cagaaatgag gagaatcagt ttgtgaggca agattggaga
    16561 taaactaata taaagcctat agcatacagc tttatggagc atctattatc caccttaagg
    16621 gaaaacaccc agattttaag acaaacaatt ttgcttacaa cgttcatgac ttgacgctta
    16681 ataagcactt cagttgctat caattttcag aaaacgattc atttaagtta atagctattg
    16741 attaattgtt gtttaaaatt ccagtaaatt ggaattgttc aagcttttga actgcttctc
    16801 gtagttcaga agcttttttt aaataatcgt cataaagttc ttcaaactcc tttgtgtttt
    16861 caatctcaat tgcaatgtat ttcttcacga atgccatccc ctttctgtgg tataatttaa
    16921 atggaaatat ggagataaaa atgtataaaa agttaataaa aatattaaat ataattcaat
    16981 tgttattagt aattattctt actggggtaa ttattttttg gatgacaaaa ttgatacttc
    17041 ttgtagattt cgactggatt acagacagtt atttcattca atctagtaaa ttcaattgga
    17101 ttaaattttt tagtcagttt ataactattg acttgatatt tgttttaccc cttatcatgt
    17161 ttaatttcgt ttactattta ctaaatgaaa aaacaaaagg aaactttggc aaattagtag
    17221 aaacagttaa tgttgtatat ccttcaattg tgtctgtgat aacactttct ctaactgcaa
    17281 atgaattagt attcactttc attacctccg ttattgcatt tattgtcctt acaatccaga
    17341 tctctaagtt tatttttaaa cagcgaaaat aaaatagaaa tcaaaaaaag cgtactgaaa
    17401 aaaatgaaca ttaatatagt tccacaaact aatacaaata atatttgaaa gactattatt
    17461 ttttacctcc tactccctca cgggagtttt tattttaaaa ctaaccaacc aatcaaccct
    17521 aaaatactta gaccgattac ccaaccacta tcaattttga ttgatagttt ttctttacca
    17581 tcttccgata tcttatgata atcataagac ccaaacaaca tttttttgat gtt
//
LOCUS       DATRSD010000044        17390 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRSD010000044 DATRSD010000000
VERSION     DATRSD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.044"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            100..1344
                     /locus_tag="VBD85_001212"
     CDS             100..1344
                     /locus_tag="VBD85_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7133769.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDRENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            1346..2629
                     /locus_tag="VBD85_001213"
     CDS             1346..2629
                     /locus_tag="VBD85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7133770.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTSYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2752..3294
                     /gene="pgsA"
                     /locus_tag="VBD85_001214"
     CDS             2752..3294
                     /gene="pgsA"
                     /locus_tag="VBD85_001214"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO7133771.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            3294..4133
                     /locus_tag="VBD85_001215"
     CDS             3294..4133
                     /locus_tag="VBD85_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7133772.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            4109..4951
                     /locus_tag="VBD85_001216"
     CDS             4109..4951
                     /locus_tag="VBD85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7133773.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDASYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            4944..5738
                     /locus_tag="VBD85_001217"
     CDS             4944..5738
                     /locus_tag="VBD85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7133774.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            5954..6493
                     /locus_tag="VBD85_001218"
     CDS             5954..6493
                     /locus_tag="VBD85_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7133775.1"
                     /translation="MSITSVKKSKPFKLGVAGLLVGASLALPLSVSAASYTVKSGDTL
                     SAIAKNHKTTVQELVSLNSISNADVISIGDVLKLDNSTASQAEAKSQPTIENSMNSSS
                     NLSSSDSAAKEEIARRESNGSYTAQNGQYYGRYQLSQSYLNGDLSPENQEKVADNYVA
                     SRYGSWSAALSFWNSNGWY"
     gene            6599..7303
                     /locus_tag="VBD85_001219"
     CDS             6599..7303
                     /locus_tag="VBD85_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO7133776.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQAYAVTETTYRPAQHQTSGQVLSNGNTAGAIGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQNQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(7438..8310)
                     /gene="sdaAA"
                     /locus_tag="VBD85_001220"
     CDS             complement(7438..8310)
                     /gene="sdaAA"
                     /locus_tag="VBD85_001220"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO7133777.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTSSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLDFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(8325..8993)
                     /gene="sdaAB"
                     /locus_tag="VBD85_001221"
     CDS             complement(8325..8993)
                     /gene="sdaAB"
                     /locus_tag="VBD85_001221"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO7133778.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            9239..10360
                     /gene="mnmA"
                     /locus_tag="VBD85_001222"
     CDS             9239..10360
                     /gene="mnmA"
                     /locus_tag="VBD85_001222"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO7133779.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10392..11003
                     /locus_tag="VBD85_001223"
     CDS             10392..11003
                     /locus_tag="VBD85_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO7133780.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            11173..13074
                     /gene="mnmG"
                     /locus_tag="VBD85_001224"
     CDS             11173..13074
                     /gene="mnmG"
                     /locus_tag="VBD85_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO7133781.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRHTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            13141..15123
                     /locus_tag="VBD85_001225"
     CDS             13141..15123
                     /locus_tag="VBD85_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO7133782.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSTTVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDIERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            15129..15581
                     /gene="rplI"
                     /locus_tag="VBD85_001226"
     CDS             15129..15581
                     /gene="rplI"
                     /locus_tag="VBD85_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO7133783.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAQELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            15624..16979
                     /gene="dnaB"
                     /locus_tag="VBD85_001227"
     CDS             15624..16979
                     /gene="dnaB"
                     /locus_tag="VBD85_001227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7133784.1"
                     /translation="MAEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            16991..17278
                     /locus_tag="VBD85_001228"
     CDS             16991..17278
                     /locus_tag="VBD85_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO7133785.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
BASE COUNT         5730 a         2721 c         3384 g         5555 t
ORIGIN      
        1 gaaatttaga attaatcata tcttggatgt ttaatctttt ttaacgcctt taaaaatgat
       61 ataatcttag ctaacaatac agaacacaaa ggatattgaa tgaaaatagt tgaaggcgtt
      121 tctctacatt taattaaaaa ccaacaattt aagactaatc atcttacttt tcgattttca
      181 ggtgatttta ataataaaac agtagcacgt agatcgctcg ttgctcaaat gttagttact
      241 gcaaatgcta agtaccctaa ggtacaagaa tttcgagaaa agttagcttc cttatatggt
      301 gctagtttat caactaagat ttctactaag ggtcttgttc acattgttga tattgatatt
      361 gtatttgtta aaaatacctt tactttagaa caagaaaata tcgttgaaca aattattaca
      421 ttcttggagg atatgctgtt ttctccttta atttctttag aacagtatca aacatctatt
      481 tttgatactg agaagaaaaa ccttattcaa tatttagagg ccgatattga agataatttt
      541 tatagcagtg acttagcact aaaatcttta ttctacaata ataagacact tcgtttacct
      601 aagtatggta cagcatcttt agtggaatca gaaaattcat ttacagctta tcaagaattt
      661 cagaagatgc ttaaggaaga tcaattagat atttttgtcg taggagattt tgatgactat
      721 cgaatgattc aggcatttaa tcgtatggca tttgagccac gtcacaaagt attagctttt
      781 gattatacgc agacttatga aaatataacg agaagtcaag tagaagataa ggacgttaat
      841 caatctatta tgcaattggc ttatcatctt ccaataactt ataaagatga agattatttt
      901 gctttaatag tatttaacgg tttatttggt gcttttgccc attccttgtt gtttacagaa
      961 attcgagaaa agcaaggttt agcctatact ataggtagtc aatttgacag ctttacaggt
     1021 ctctttacga tatatgcagg tattgataga gagaatcgtg agcgtttttt gaagcttatt
     1081 aataagcaat tcaataatat taagatgggc aggttctctt ctacattatt gaaacaaaca
     1141 aaagatatat tgaaaatgaa ctatgtttta gcttctgata atcctaaggt catagttgac
     1201 cacatttatc atgaacatta tttagatcag tttcatactt cagcactatt cattgacaaa
     1261 gtggacgatg taacaaaatc agatattgtc agcgtagcaa caaaattaaa attacaagct
     1321 ttttattttt tggaaggaaa ctaggatgac agttaataaa ataacttatc agaaccttca
     1381 ggaggaagta tataagttaa cattagagag cgggctaaat gtatacctta ttccaaaacc
     1441 atcatttaag gaaactgttg gagtattgac agcaaatttt ggatcactcc atacaagata
     1501 tacgaggaat ggttgtgtag aacattatcc ggcaggaatt gctcacttct tagaacataa
     1561 attatttgaa ctggataaag gacaagacgc tgcaactcaa tttactaaat atggcgccga
     1621 gagtaatgcc tttacaactt ttgataaaac tagtttttat ttttcaacta taagtcacat
     1681 tacaaactgc ttagatatac tacttgactt tgtattgaca acaaacttta cagaagagtc
     1741 aattactaaa gaaaaagaca ttatcaaaca agaaattgaa atgtatcaag atgaccctga
     1801 atataggctt tatcaaggag tgttatcgaa cctttatccc aactctccat tagcatttga
     1861 tatcgctgga gattaccaat caatctctca aataacctta accgatttac aagaaaatca
     1921 caaagacttt tatcagttgt ctaatatgaa tttagttctt gttggacaat ttagtccaca
     1981 agaaattata acttaccttc aaaaaaattc tcatttaaca agttactcac agaatattga
     2041 ccgagattct attagtcttg aacctgttat aaaaaacaac tcttgccata tgacagtgac
     2101 aaaacctaaa ttagctattg gttatcgaaa atcaagtcat atgagacatg gttcttacct
     2161 aaaagagaaa atcggattac agctattttt tgcaatgctt ttaggatgga cttctacaat
     2221 taatcaagac tggtacgaat ctggtcaaat tgatgactct tttgatatag aaattgaagt
     2281 tcatccagat tttgagtgcg ttattatttc tttagatacg acagagccaa ttgctttttc
     2341 aactcagttg aggttattgc taaaaaatgc tttgcaatca tctgatctta ccgaaagcca
     2401 cttacaaaac gttaagagag agctctatgg cgatttttta aggagcttag attctattga
     2461 aaatctcgca atgcaattcg tcacctattt atatgatgga aaaacgatgt atttagattt
     2521 gccatctatc gttgaagaat tagacttgga agatgtcatt acgattggta aggatttctt
     2581 agataatgct gacacatctg acttcgttat ttttccaaaa tcttcgtaaa taatctgaaa
     2641 ttttgatata ataaggaagc ttagacatct ataaagttgg tatgtcaaaa gtcttttact
     2701 agttttgaaa taacataata taggaatact atgtttccaa aaggagaatt tatggtgaag
     2761 aaagaaaata ttccgaatct attaactgta gtcagaattt taatgattcc gttatttata
     2821 gtattaactt cagtaacaac aagtacaact tggcatattg ttgcagctat tgtatttgct
     2881 attgcaagct tgaccgatta cctagacggt tatctagcac gtaaatggca agtagtaaca
     2941 aattttggga aatttgcaga tccattagct gataaaatgc tagttatgag tgcttttatc
     3001 atgttagttg gactagactt agcaccggct tgggtttcag ctattatcat ttgtagagag
     3061 ctagcagtta caggtttgcg tttactttta gttgaaacag gtggaacagt acttgctgca
     3121 gctatgccag ggaaaataaa gacggctact caaatgtttg cagttatttt tttgttagta
     3181 cattggatga cacttggtaa catcatgtta tacattgctt tattctttac cttgtattca
     3241 ggctacgact attttaaagg tgcaggcttc ttatttaagg atacttttaa ataatgacaa
     3301 atattattac cgttaataat ctctttttta aatatgatag taatcaaaca cattatcaat
     3361 tggagaatgt atcgtttcat gtgaaacaag gagaatggct atctattatt ggtcataatg
     3421 gttctgggaa atccactact gttcgtttaa tagatgggct tttagaggcg gagtcaggcc
     3481 agattatcat tgatggacaa gagttaaccg aagataatgt ctgggagtta cgtcacaaga
     3541 ttggcatggt atttcaaaat cctgataacc aatttgtagg ggcaactgtt gaagatgacg
     3601 tcgcctttgg tttggaaaat aaaggtattc ctttgaaaga catgaaagaa agagtggacc
     3661 aagcacttga tttggtaggc atgtctgaat ttaaaatgag ggagcctgca cgtttatctg
     3721 ggggacaaaa gcagcgtgta gccattgcag gagctgtagc tatgcgtcca caagttatca
     3781 tcttggatga ggcgactagt atgcttgatc ctgagggacg attggaactg ataagaacga
     3841 tacgagctat tcgtcaaaaa tataatctta ctgttatttc aattacacac gacttagatg
     3901 aagttgcatt gagtgatcgt gtcatcgtta tgaaaaatgg taaagttgag tcgacatcca
     3961 ctccaaaagc gttatttggt cgtggaaatc gtttgattag tttaggttta gatgttccct
     4021 ttacaagtag gttaatggca gaactggctg ctaatggtct tgatataggg acagagtatc
     4081 ttacagagaa ggaattagaa gaacaattat gggaattgaa tttaaaaatg taagttatac
     4141 ctatcaagcc ggcactcctt ttgaagggcg tgcccttttt gacgtcaatc tgaaaattga
     4201 agatgcttcc tataccgcgt tcattgggca cacaggttct ggaaaatcaa ctattatgca
     4261 acttttgaat ggtttacata ttcctacaaa aggtgaggta attgtcgatg atttttctat
     4321 taaagcaggg gacaagaaca aagaaatcaa atttataagg caaaaagttg gtttagtttt
     4381 tcaatttcca gaaagtcagc tttttgaaga gacagtttta aaagatgttg cttttggacc
     4441 acaaaatttt ggtatttctc agattgaagc tgaaaggctg gctgaagaaa aattaaggtt
     4501 agttggtatc agtgaggatt tattcgataa aaatccattt gaactttctg gagggcagat
     4561 gaggcgggtt gctatagctg gtattttagc gatggaaccc aaagtactag tactagatga
     4621 gccaacagct ggacttgatc ctaagggaag aaaagaatta atgactcttt ttaaaaatct
     4681 tcataaaaaa ggaatgacta tcgtcttagt gactcactta atggacgatg tagcggatta
     4741 tgctgactat gtgtatgttt tagaagcagg gaaagtaacc ttatcaggac aaccaaagca
     4801 gatttttcaa gaagtagaac ttttagaaag taaacaatta ggagttccca aaatcaccaa
     4861 gtttgctcaa aggctatctc ataagggatt aaatttacct agtttaccaa ttactattaa
     4921 cgaatttgtg gaggctatta agcatggata aattgatttt gggacgttac ataccaggta
     4981 attctctcat tcataaatta gatcctcgaa gtaagttact agccatgttg ctttttatca
     5041 ttattgtttt ttgggcaaat aatgtagtga caaatgtgat cgtattcatt tttacgttgg
     5101 ttattgtggg tttatctcag ataaaatttt cctatttttt caatggtatt aaaccgatgg
     5161 ttggtattat tttatttaca accctgttcc agatgctgtt tgcacaaggc gggcaggtta
     5221 tcttctcgtt ttggattttt agcatcacta gccttgggtt acaacaagca gcacttattt
     5281 ttatgagatt tgttttaatt atcttctttt cgacattact taccttaact actactccgt
     5341 taagtttagc agatgctgtt gaatctttgt taaaaccatt ggaagtactg agagtaccag
     5401 cacacgagat tggtttgatg ttatccctta gcttacgttt cgttcctact ttaatggatg
     5461 atacaacacg tatcatgaat gctcaaagag ctcgtggagt tgattttgga gagggaaacc
     5521 taattcataa ggttaagtcc attatcccta ttttgattcc attatttgct tctagtttta
     5581 aaagagcaga tgcgttggca attgctatgg aagcaagagg ttatcaaggc ggagccaatc
     5641 gtagtaagta ccgtttattg aagtggactg ttcgggatac ttttagtata ttactgatgt
     5701 tattattggg tttgagtttg tttctattaa aaaattaaaa ggttaaggtc agtttatggt
     5761 cttagccttt tcatttatat aatgtgaaaa gctcaacaac tttacttaaa gaaaactaaa
     5821 aattgttttt tggaggcctt ttctttatta atttacaatt tatttgtaat aaatttcatc
     5881 taaggcaaaa caattagtaa tatttacaag gtatgatagt acgcgtatca attaatgaaa
     5941 gagagcatat attatgtcaa taacctcggt taaaaaatca aaaccattta aattaggagt
     6001 ggcaggtctt ttagtgggtg cttcattagc tttaccactt tcagtaagcg cagcatctta
     6061 taccgtgaaa tcaggtgata ccttatcagc tattgctaaa aatcataaaa ctacggtaca
     6121 agagttagtg tctctcaata gtatcagtaa cgctgatgtc atcagtatag gtgatgtttt
     6181 aaaattggat aattctacag ctagtcaagc agaagcaaaa tctcaaccaa caattgaaaa
     6241 ttcaatgaat tcttcatcaa atttgagttc aagtgattca gccgcaaaag aagaaatagc
     6301 tcgtcgtgaa tcaaatggta gttatactgc acagaatgga caatattatg gaagatatca
     6361 actgtctcaa tcttacctaa atggcgactt atctcctgaa aatcaagaaa aagtagcgga
     6421 caattatgtg gcttctcgtt acggatcttg gtcggcagcg ctatcatttt ggaatagtaa
     6481 cggttggtat taataaaagt tgataaaaca aagagattta cttaaaatta aatctctttg
     6541 taatattttt aacatctatt caattgcaag gtaacaaata aagtataata taggtaacat
     6601 gaataaaaga agaaaattat caaaattgaa tgtaaaaaaa caacatttag cttatggagc
     6661 tatcacttta gtagcccttt tttcatgtat tttggctgta acggtcattt ttaaaagttc
     6721 acaagttacc actgaatctt tgtcaaaagc agataaagtt cgcgtagcca aaaaatcaaa
     6781 aatgactaag gcgacatcta aatcaaaagt agaagatgta aaacaggctc caaaaccttc
     6841 tcaggcatct aatgaagccc caaaatcaag ttctcaatct acagaagcta attctcagca
     6901 acaagttact gcgagtgaag aggcagctgt agaacaagca gttgtaacag aaaacacccc
     6961 tgctaccagt caggcacaac aagcttatgc tgttactgag acaacttata gacctgctca
     7021 acaccagaca agtggccaag tattgagtaa tggaaatact gcaggggcta ttggctcagc
     7081 agctgcagca caaatggctg ctgcaacagg agtccctcag tctacttggg aacatattat
     7141 tgcccgtgaa tcaaatggta atcctaatgt tgctaatgcc tcaggagctt caggactttt
     7201 ccaaacgatg ccaggttggg gttcaacagc tacagttcag aatcaagtta attcagctat
     7261 taaagcttat cgtgctcaag gtttatcagc ttggggttac tagtgaatag ctataaatta
     7321 actttacact gttgtcaaca ataattacag attgctaaaa caaataaata aaaacgctac
     7381 agctttactg tagcgttttt atttattttc agggaaagag tagaaaattg ataaacttta
     7441 ttccccaaaa atttctttgc tataacgtct tcctgtcggc gtggcagcaa gtcctccttc
     7501 tgcagtttca cgaaaagcag tcggtaaact tgatccaact tgatacattg catcaataac
     7561 ttcatctact ggaatttgcg attcaatacc agccaaggcc atatcagcag caacaagtgc
     7621 aaaacttgat ccaagagcat tccgcttcac acaagggact tcaactaaac ctgcaacagg
     7681 atcacagata agtccaagca tatttttaat aacaaatgct atagcttggc tagcttggaa
     7741 aggagtacct ccagcagcca taactaaagc agccgcagcc atagcactag ctgacccaac
     7801 ttcagcttgg caacctcctt ctgcacctga gatagaggca ttattaccaa tgacgagacc
     7861 aaatgcgccg gctgtaaata gaaaatcaag ttgctcttct tctgttaaat taagcttttc
     7921 aatggctgta gaaatcacgg ctggtaaaca tcctgcacta cctgcagttg gtgttgcaca
     7981 gaccagtccc atcttagcat tcaactcatt aacagccata gcattcctaa cggcagctag
     8041 gattgtggta tctgaaatag tttttcctga ttgtaaatat tgatccatct tgacagcatc
     8101 accgcctgtt aagccactga ttgatttact agaggttaat ccatcaataa cagaagcttt
     8161 cataacttca aggtttcggg acataatata acgaatttct tcacgacttc taccagtcat
     8221 ttcaatttcc gtttggatca tgagctctgc tatgttaccc ttatgttggc tattagcttg
     8281 ctctaccagc tcttcaattg tataaaacat agttaccctt tctcttaatc aaagaagttg
     8341 acattgtgta aatgaggaat agcttctatc tttttgacag cttgttgaca atctcgtgaa
     8401 tctacttcta taatcatgat agctttttct cctgcacttt cgcgtgttac attcatttga
     8461 gcaatattaa tgttaaagtc agataaaata tcagttactt tagcaatcat gccaggtata
     8521 tcttgatgaa cgataattaa agtaggagta ttcattgtta atgaaactga gaatccattc
     8581 aactcagtaa cttggatgtt tccgccacca attgacactc ctgtaatact catgctccta
     8641 tccccatttt taacagttat tttcgcagta tttggatgtg gtgaattact atcttttagg
     8701 atatcccagt aaatcttaat tcctttttga tgagcaattt caagagagtt tttaatatct
     8761 ggattatctg tatccattcc tagaatccct gcaaccaatg ctttatcagt accgtgtcct
     8821 tggtaagttt tagcaaaaga attgtataaa tgaaaggtta cttcactagg ttcaccaaaa
     8881 atagaatgga caactttacc aatgcggaca gctcctgcag tatgactact tgatggtcca
     8941 atcataacag gaccaataat atcgaagacc gattgaaatt ttaaatgttt catagttttt
     9001 cctcgtgatt tttgtacatt ctcattataa cagattattt ctaaaaaata tcataaaaag
     9061 ataaaaaaac tgcaatgtaa taaatattta tcacaacaaa aacatacaac ttgacaacaa
     9121 atagtgctat aatatataag cacttttata gtgtttctta gtcaatatga ttgatctaag
     9181 aacaacttgg cccttcaagc ctaagttaaa aataaaactt tttgatggga gaattcttat
     9241 gactgataat tcaaacattc gtgtcgttgt aggcatgagt ggaggtgttg attcatcagt
     9301 aactgccctg cttttaaaag agcaaggtta tgatgttatt ggtgttttta tgaaaaattg
     9361 ggatgacaca gatgaatttg gtgtttgtac ggctactgaa gattataaag atgttgctgc
     9421 tgttgctgat cagatcggca taccatacta ctctgtcaac tttgaaaaag agtattggga
     9481 cagggttttc gaatattttt tagcagaata tcgagcagga cgtacgccaa atccagatgt
     9541 tatgtgtaat aaggaaatta aatttaaagc atttctagat tatgcgatga cattaggtgc
     9601 tgactatgta gcaacaggtc attatgcaca agtaacacgt gatgagaacg gaatagttca
     9661 tatgcttaga ggagcagata ataataagga tcaaacttat tttctaagtc aattatctca
     9721 ggagcaattg caaaaaacgt tatttccact tggtcattta caaaaaccag aagttcgccg
     9781 tatagctgaa gaagcaggac ttgcgaccgc taagaaaaaa gactctactg gtatttgttt
     9841 tattggagaa aaaaatttca aggatttcct gggtcaatat ttaccggctc aaccaggtcg
     9901 tatgatgact gttgatggtc gtgacatggg tgaacatgct ggtttaatgt attatacaat
     9961 tggtcaaaga gggggccttg gtattggtgg gcaacatggt ggagataata aaccttggtt
    10021 tgtagttgga aaggatttaa gtaaaaacat tctttatgta ggtcaaggtt tctatcatga
    10081 ctcattgatg tcaactagct tgacagcttc tgaaatccac tttacacgtg atatgcctaa
    10141 tgaatttaag ctagaatgta cagctaagtt cagatatcgt caacctgaca gtaaagttac
    10201 agtttacgta aaaggtaatc aagcaagggt tgtgtttgat gatttacaac gagcaatcac
    10261 accaggtcaa gctgttgttt tttataatga acaagaatgt ttaggcggtg ggatgattga
    10321 tcaggcttat cgtgatgata aaatctgtca atacatttaa ttgacactta attattaagt
    10381 gaggtaatcg tatgatttca aaatttattt tagctttcat ggcttttttc gccattatga
    10441 atcccatttc taatttgcca gcttttatgg ctttagtagc tgatgatgat cagaagattt
    10501 cacgtcggat tgcagcaaaa ggagtattac ttgcttttgt cattattgta atatttgtat
    10561 taagtggcca cttacttttt aacttatttg ggattaccct agccgcccta aaaatatctg
    10621 gtggaatatt ggtaggcatt ataggttata aaatgattaa tggtatacat tcaccaacca
    10681 ataaaaacct agaggagcat aaggatgatc caatgaatgt ggcagtttca ccattagcaa
    10741 tgcccctttt ggcaggtccg ggtactattg caactgctat ggggttatca agtggtggtt
    10801 tatctggcaa actcattact atacttgctt ttgccatact atgtgtgata atgtatgtta
    10861 ttttaataag tgcaaacgaa attactaaat ttcttggaaa aaatgcaatg actataatca
    10921 ccaaaatgat gggacttatt ttgatgacta tcgggataga gatgctaata acaggcataa
    10981 aaattggttt tcatttaaca tagcctatcc cctctggttg acttttaaaa gataaatgct
    11041 aaaataaatt taacaataac tatgatggtc aaagttcatg gaatttgtaa gtctttttga
    11101 cgtacaattt tcgagagttt tggctatttt tgtgttttaa tgaattagac tttgaaattg
    11161 gaaaggaata aaatgacaca taattttgca gaaaactatg atattattgt tgttggagcg
    11221 ggtcatgcag gtgtggaagc tagtctagct gctagtcgta tgggatgtaa gacacttcta
    11281 gctactatca acttagaaat gttggcattt atgccttgta atccttctat aggtggctct
    11341 gctaaaggta tcgttgtcag agagattgac gctttaggtg gtgaaatggg taaaaacatt
    11401 gataaaacct atatccaaat gaaaatgctt aatacaggta aggggcctgc tgtccgtgcc
    11461 ttaagagcac aagctgataa agccctctat gcacaaacaa tgaagcaaac tgtagaaaag
    11521 caagaaaacc ttaccttaag acaagctatg attgacgaaa tacttgttga ggatggaaag
    11581 gttgtggggg ttcgtacagc aactaatcag aaattttcag caaaatctgt tgttataaca
    11641 acaggtactg ctcttcgagg agagattata ttaggtgagc ttaagtactc ttcgggccct
    11701 aataatagtt tagcttctgt gacattggca gataatttaa gagacctagg attagaaatt
    11761 ggtcgtttca aaactggaac acctcctcgt gtaaaagcct cctctattaa ttatgaaaag
    11821 acagagatac aacctgggga tgagcaacca aatcattttt catttatgtc tagagatgaa
    11881 gattatatta ctgaccaggt accgtgctgg ttgacctata cgaatacttt gagtcatgac
    11941 atcatcaatc aaaatctaca ccgtgctcct atgttttctg gaatcgttaa gggagttggt
    12001 cctcgatatt gtccgtcaat tgaagataaa attgtgcgtt ttgctgataa agagcgccac
    12061 caactcttct tagaaccaga aggtagacac actgaagaag tttatgttca agggctatca
    12121 acgagtcttc ctgaggatgt tcaagttgat ttattacgtt ctataaaagg gcttgaaaat
    12181 gctgaaatga tgcgtacagg ctatgctatt gagtatgata ttgtattgcc tcatcaatta
    12241 agagcaacac tcgaaacaaa agtaattgct ggtttgttta ctgcaggaca gactaatggt
    12301 acttcaggtt atgaagaagc tgctggtcaa gggttggttg ctgggatcaa cgctgctctt
    12361 aaagttcaag ggaagccaga acttatttta aaacgaagtg atgcttacat tggtgtcatg
    12421 attgatgatt tagttaccaa agggactcta gaaccatatc gtctgttgac aagtcgcgca
    12481 gaatatcgtc tgattcttcg tcatgataat gcagatatgc gtttaacaga aatcggttat
    12541 gagattggcc tagtagatga agagcgttat gcgatattta aaaagcgtca aatgcaattt
    12601 gaaaatgaat tagaacgttt agactctata aagttaaagc cagtttctga gacaaataaa
    12661 cggattcaag aattgggctt taaacctttg acagatgctt taactgccaa agaatttatg
    12721 cgccgcccac aaatcacata tgcagtagca acggattttg ttggttgtgc tgatgagcca
    12781 ttagacagta aggttattga acttttggaa actgaaatta aatacgaagg ctatattaaa
    12841 aaggctttag atcaagttgc aaaaatgaaa cgaatggaag aaaaacgtat tccccctcat
    12901 attgactggg acgacattga ttctatcgca acagaagcta gacaaaaatt caaaaaaatt
    12961 aacccagaaa cgctaggtca ggctagtcgt atttcaggag ttaatccagc cgatattagt
    13021 attttaatgg tttaccttga aggaagacaa aaaggtcgta aaaatatcaa ttgataatag
    13081 tgttcgcccc tgtttatcag gggcgttttg tgttataatt aggtcttaag aggttattta
    13141 atgaaaagat ttcgttttgc aaccgtccat ttagttttaa taggcctgat tttatttgga
    13201 ctattagcta tttgtgttag gttatttcaa tcctatacgg ctctattatt ggctattttt
    13261 gttgctttat cttttgttgt tgccttatta tattaccaaa agataactta tgaactatct
    13321 gaagttgaac aaattgaatt acttaatgat caaacagaag tttctcttaa gagtcttctt
    13381 gaacaaatgc cagttggtgt tatacaattt gatcttgaaa caaatgatat tgagtggttt
    13441 aatccttatg cagaattgat ttttactggg gataatggcc attttcagtc tactactgtt
    13501 aaagatatca ttactagtag acgtaatgga acggctgggc aatcatttga atacggtgat
    13561 aacaaatatt cagcttattt ggatacagag acgggtgttt tctatttctt tgataatttc
    13621 atggggaata gaagaaacta tgatagttca atgctacgtc cagttattgg tattatttcc
    13681 attgataact acgatgatat tatggatact atgttagagg ctgatatgtc aaagattaac
    13741 gccttcgtga ctagttttat ttctgatttt acacagtcta aaaatatttt ttatcgtcgt
    13801 gtcaatatgg atcgatacta tatttttacc gactatagcg ttttaaatac attgataaaa
    13861 gataaatttg atattttaaa tgagtttcga aaacgcgctc aggaaaacca tttatcatta
    13921 acattaagta tgggaatttc ttatggtgat ggtaaccata atcaaattgg gcaaattgct
    13981 ttagaaaatc ttaatacagc actcgttcgc ggaggagatc aaatagtagt acgtgaaaat
    14041 gatagtagta aaaaagcact ttactttggt ggtggtgctg tttcaactat taaacgttca
    14101 cgaacacgta ccagagctat gatgactgct atttctgacc gtttgaaagt agttgatagt
    14161 gtctttattg tgggacacag gaaattggac atggatgcac taggtgcatc tgtagggatg
    14221 caattttttg ctagtaatat tgttaatgcc agttatgtag tttatgaccc aaatgacatg
    14281 aattctgata ttgaaagagc aattgattac ttgcaagaag acggcgaaac acgtctcgta
    14341 agtgttgaga gagcttttga actaattact caaaattctt tattagtcat ggtagaccat
    14401 tctaaaacag ctttaacctt atctaaggaa ttctttaaca agtttgcaga tgttattgtg
    14461 gtagatcatc atagaagaga tgaagatttt cccaaaaatg ctgtcctttc atttatagaa
    14521 agtggcgcaa gtagtgccag cgaattagtt acagaattga ttcaatttca acaagctaaa
    14581 gataaattaa gccgcagtca agcaagtatt ttaatggctg gtatcatgct tgatacaaga
    14641 aattttgcat ctaatgtaac gagtagaact tttgatgtgg caagctattt acgcggttta
    14701 ggtagtaata gtatggctat tcaaaaaata tcagctacag attttgatga atatcgttta
    14761 attaatgaat tgattttaaa aggtgagcgt atttatgata acatcattgt tgctaccgga
    14821 gaagaacata aagtttactc gcatgttata gctagtaaag cagctgacac catgttgaca
    14881 atggcaggta ttgaggctac ttttgttatt actaaaaaca gttcaaatat tggtatttca
    14941 gcgcgcagta gaaataatat caatgtccag cgcattatgg aaaaacttgg tggtggcgga
    15001 cattttagtt ttgctgcttg tcaaatacaa gacaagtctg taaagcaagt tagacgcatg
    15061 ttacttgaaa taattgacga agatttaaga gaaaatagta cagtcgaaaa tagaagggat
    15121 taattgagat gaaagttatt tttttacaag atgttaaggg gaaaggaaaa aaaggtgagg
    15181 ttaaggaagt acctactggt tatgctcaaa acttcttatt aaaaaagaat ctagctaaag
    15241 aagcaacgac acaagctatc ggtgaattaa aaggaaaaca aaagtccgaa gaaaaagcgc
    15301 aagcagaaat tttagcacag gcacaggagc tgaaaacaca attagaatcc gaaactacac
    15361 gagtgcaatt tattgaaaag gtaggtccag atggtcgtac gtttggttct atcacagcca
    15421 aaaagattgc tgaagagtta caaaaacaat acggcattaa aattgacaaa cgtcatattg
    15481 atttagatca tactattaga gcgattggta aggttgaagt accagtaaaa ttacataaac
    15541 aggtttcttc acagattaag cttgatatca aagaagcgta aactgagaga tgataaaggt
    15601 tgacaagtca ggaggaatga agtttggcag aagtatctga gctaagagta caacctcaag
    15661 acctgttggc agaacaggcc gttttaggat ctatctttat ctcaccagag aaactgatta
    15721 tggtgagaga atttataagt ccagatgatt tttataaata ttcacacaaa gttatcttca
    15781 gagcgatgat taccttagct gatagaaatg atgcaattga tgctgctact gttagaaata
    15841 ttttagatga ccaaggtgat cttcaaaaca ttggtgggtt gggctatatt gttgaattag
    15901 ttaatagtgt tccaacaagt gctaatgcgg agttttatgc caaaatagtt tctgagaaag
    15961 ccatgttgcg agatatcatt tctaagttga cagatactgt caatatggcc tatgaaggaa
    16021 atgattctga tgaaattatt gctaccgctg agaaggcttt agttgatatt aacgaacaca
    16081 gtaatcgtag tgggtttaga aagatatctg atgttttaaa agttaattac gaaaacttag
    16141 aattacgttc acagcaaacc tcagatgtca caggtttacc aactggattt agggatttgg
    16201 acaggattac tacaggttta catccagatc aattaatcat cttagcggct cgtcctgcag
    16261 taggtaaaac agcctttgtt ttaaatattg ctcaaaatgt tggaactaaa caaaatagac
    16321 ctgtagctat tttttcactt gaaatggggg ctgagagttt agttgaccgt atgttggcag
    16381 cagagggaat ggttgattcc catagtttac gaactggtca gttgacggat caagattgga
    16441 ataatgtaac gattgctcaa ggagctttgg cggatgcgcc aatttatatt gatgatacgc
    16501 ccgggattaa aattacagaa attagagcac gctctaggaa gttatctcaa gaagttgatg
    16561 atggtttggg tttaatcgtc attgactacc tccaattaat ttcaggaact agaccagaaa
    16621 atcgtcagca agaagtttct gaaatttcaa ggcagttgaa gattttagct aaagaattaa
    16681 aggttcctgt tatcgcatta agccagctct ctcgtggggt tgagcaaagg caggataaac
    16741 gaccggtctt atctgatatt cgtgaatctg gatcgattga acaagatgct gacattgttg
    16801 ctttcttata tcgagatgat tattatcgta gagagggcga agaagcagag gaaattgtag
    16861 aagataatac cgttgaggtt attttagaaa agaaccgtgc tggtgcgcgt gggacagtaa
    16921 aattaatgtt tcaaaaggaa tataataaat tttctagtat agctcaattt gaagaatagg
    16981 agatgttaat atgagtgatg catttgcaga tgttgcaaaa atgaaaaaaa taaaagaaga
    17041 tatcaagtca catgagggac agatggtcga acttactctc gaaaatggtc gtaagaggga
    17101 aaaaaataaa attgggcgcc taattgaagt ttacccatct ttgtttatcg tagagtataa
    17161 ggatactgca gctgtaccag gagcaattga taatacttat gtagagtctt acacttattc
    17221 agatatttta actgagaaaa ctttaattcg ttattttgat gacgagtcag cagaataata
    17281 tgaaaacctc gactgaggtt ttttgtttct ataaaaaaat ttactaattc tctattggtg
    17341 gttagaatta agaatttttc ttattataat cggaaatact tattagatat
//
LOCUS       DATRSD010000045        17254 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRSD010000045 DATRSD010000000
VERSION     DATRSD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.045"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(16..1320)
                     /locus_tag="VBD85_001229"
     CDS             complement(16..1320)
                     /locus_tag="VBD85_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7133786.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQGQAVSQAAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQS
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTT
                     TSTATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENARLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(1467..2366)
                     /locus_tag="VBD85_001230"
     CDS             complement(1467..2366)
                     /locus_tag="VBD85_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO7133787.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(2559..4106)
                     /gene="purH"
                     /locus_tag="VBD85_001231"
     CDS             complement(2559..4106)
                     /gene="purH"
                     /locus_tag="VBD85_001231"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO7133788.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVSLDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKASPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAGKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(4126..4878)
                     /locus_tag="VBD85_001232"
     CDS             complement(4126..4878)
                     /locus_tag="VBD85_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133789.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRAIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVVYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLAEKLGYEFSHEYIAYEID"
     gene            complement(4898..5449)
                     /gene="purN"
                     /locus_tag="VBD85_001233"
     CDS             complement(4898..5449)
                     /gene="purN"
                     /locus_tag="VBD85_001233"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000685111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO7133790.1"
                     /translation="MKIAVFASGNGSNFQIIAEQFPVSFVFSDHRDAYVLERAQNLTI
                     PSFAFELKEFENKAAYEQAIVNLLDKHEIDLVCLAGYMKIVGEALLSAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGIKRKQ"
     gene            complement(5617..6639)
                     /locus_tag="VBD85_001234"
     CDS             complement(5617..6639)
                     /locus_tag="VBD85_001234"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO7133791.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGNEVLPELEGKQLK
                     DVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFNAIEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(6667..8121)
                     /locus_tag="VBD85_001235"
     CDS             complement(6667..8121)
                     /locus_tag="VBD85_001235"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO7133792.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIIIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            complement(8355..12080)
                     /locus_tag="VBD85_001236"
     CDS             complement(8355..12080)
                     /locus_tag="VBD85_001236"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO7133793.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKELRIVQVYDV
                     FHLAEDLLARAEKHIFSEQVTDRLLTEAEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGGDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYTADDFVAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELDRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPETGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVIAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFKRMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRRVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIAGAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVAFVTLNEAAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGFIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(12234..12941)
                     /locus_tag="VBD85_001237"
     CDS             complement(12234..12941)
                     /locus_tag="VBD85_001237"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO7133794.1"
                     /translation="MTNQLIYTGKAKDIYSTKDENMIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGINLIDFKLEFGFDNDGKIILADEFSPDNCRLWDAEGNHMDKDIFRRDLG
                     SLTDAYQVVLDKLKTLD"
     gene            complement(13065..13304)
                     /locus_tag="VBD85_001238"
     CDS             complement(13065..13304)
                     /locus_tag="VBD85_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7133795.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPETSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(13315..14307)
                     /gene="plsX"
                     /locus_tag="VBD85_001239"
     CDS             complement(13315..14307)
                     /gene="plsX"
                     /locus_tag="VBD85_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO7133796.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEIPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DVVVTDGFTGNAVLKTMEGTAMSIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDNMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(14385..15263)
                     /locus_tag="VBD85_001240"
     CDS             complement(14385..15263)
                     /locus_tag="VBD85_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7133797.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFVPILASILLFLIYFQKRDFTSLGFTKDNNILTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLFLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVT
                     GVHTTWNFTMGCVLGNKVSGGYSPVSLFRITENSSFALWNGGDFGFEGGVLCSLILVG
                     CLAYFSLERVRVSVRY"
     gene            complement(15326..16087)
                     /gene="recO"
                     /locus_tag="VBD85_001241"
     CDS             complement(15326..16087)
                     /gene="recO"
                     /locus_tag="VBD85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO7133798.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQEINSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLHLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(16077..17252)
                     /locus_tag="VBD85_001242"
     CDS             complement(16077..17252)
                     /locus_tag="VBD85_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7133799.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQEYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
BASE COUNT         5249 a         3805 c         3024 g         5176 t
ORIGIN      
        1 gcttcctttt ttatattatt tgttaaatga tacgtgaacg tggtcatagt ggttggcagt
       61 aacgccacca cgatctggca ttgcattcca agtattagca ggtccataaa tactatttgt
      121 atttgagtaa aacttttgtt gccagataac atatgaaatg ttatttgctg ccatattttg
      181 tgtagagtac tgtgcaactt cattaccaag tgcttggttt ttacctacaa taaagtcaac
      241 tgctaaacct ttaccatgat cacctggatc tcccgcacgg tatgtactga attcattaac
      301 tccataagtt gacgctactt tttctttata agctgcaaca tgaggttgga gccttgcatt
      361 ttcaggatgt gcagctactg catttgttgt tgaagctggt tgtgctaccg gtgttgctgt
      421 tggagctttt tgtgctaccg gaacgctctt aacttcagtc gcttgtaact tactgtctgt
      481 agctgttgaa gtcgtagtca caggaactgc tggagctgat acatgctctg gtgatgcacc
      541 agtttctact ttaggagtga ctactttaac acttgccact ctaggggctg ctacagttct
      601 taccggtgct actttagcta ctggagctgg tgtttcagcg gcaacagaag ctggtgatac
      661 tgttgttgac tgactgactg acgtctgagt tggtttaact tcctctttag ctgctggaac
      721 ttctgaagta atcgacttca caggagctgg tgatacctgt tcattagctg ctgcttgact
      781 aacagcttgc ccttgtgcta atacttcttt tgatttcaaa gctggcgcag aagaatatgt
      841 cttcattggc gaaacaatcg ttgttgctgc ttctggtgtc ataccttccg aaattgtatt
      901 gagagaaact ttttggtctg caacagaaac ttgattggtt ttcaaatcga cagtagctgt
      961 tgtttgacca gcagcatttg ttgctggtgt ttctattttc attgaagtag cagtatgact
     1021 cttctgatcg taagttactg tcagtgttgt ctcaggataa ataagattga tatctgcaat
     1081 gttattaatt tttgctaaga cattcatatc aattgacatt gcttctgaaa taacgcttag
     1141 tgtatcacca tatttcacag tatatgatga tttattgtct tgctttacca aatcagcctt
     1201 tacctctgaa acagtacgtg ctgtccacgt cgtatctgtt tcttgtgctt gaacacttgc
     1261 gactgataat agcgaagctg ccattgtcga tgtcaatagt acctttttat tcattttcat
     1321 tgcttcaatt cctatttcct ttttattagt ccgagactat aatatcacac tttactggac
     1381 tataaaatta ttttcagacg gtttaaagaa attgtactta gttagtaaca aaactgtcat
     1441 ttaaaacaat gaatcaatac ctcaaactat ttgtaaagca gttcttgact gttgtcaacg
     1501 cgaagccaaa ctgaactacc attagcaaag cgtgtcttag cccaagttaa accagctgtt
     1561 ccttggatgg gtttttctac agtcttaaga gttttaggat taaagataaa gtaaccacct
     1621 ttttgaagaa cctggtcagc tgtcaaatta ccattagcat caacctcatc aatttctgat
     1681 gctggtatac cattatcgtt ccaatcaaac ccagtcggcg ttagggtgtt atttttgact
     1741 aaccaaacac cattaacctt ttgtaattca tctactcgat acacttttaa tttactagat
     1801 tgattctgta caggagctga ttgtaatggc ttaatgcttg gagctgatgc ttgcgttttt
     1861 ccactaaagg tcgcaacatt agcaattagt gacgttggat tgatacgtcc atggaaacca
     1921 ttttgaaaat tagggttagc tggtaaaaat tcaaaatgaa ggtgaggtcc cgtcgccata
     1981 ccagttgctc ctacgtaacc gatgatatct ccttgtttga ctttttcccc agtcctagcc
     2041 accacacgtg acatatgagc gtaaccacta tgcattccat ccgcatgttg aatcatgaca
     2101 caatttcctg ctaagtctgt catccaagaa aagttggctc cagctcctgc aaatttcaca
     2161 gtaccatctg ccactgccct aataatcgtt ccagtcggaa cagcataatc caccccacaa
     2221 tgtccaggat aaccattgaa acctgttgta attctaccat tatcaattgg acggacataa
     2281 gtatccgcta aaactcggga acccgcagat aaaaccatac cacctaaaac aactaaggaa
     2341 cttgccttaa ctaaccattt attcaagaaa aacctccaaa tagaattaat ataatgaagt
     2401 gctcaaacac ttgcaattaa cttattcgaa aatttaaatc aaaatatcaa aaaaagtact
     2461 caccttgatg agtacttagg aggtttaata aaaagaaaag tttttaggat atacgtagta
     2521 tacctgagtt aacttaagaa aacctaaact actatttttt aatgtctaaa atgtctcacg
     2581 cctgtgaaga tcatggtcaa gccatgtttg tttgcggcgt caatagattc ttggtcacga
     2641 actgaaccac ctggttggat gattgctttg atccctgcgg cagcgatttc ttcaatgttg
     2701 tccgcaaatg ggaagaaggc atctgatgct agaacggcac cgtcaaggtg gtccttagcc
     2761 tgctcgatag caatcttaac tgagccgaca cggttggttt gacctgcacc gagtccaagc
     2821 gtcatgtggt cgttagtgat gataatcccg ttagatttaa catacttgat agccttccag
     2881 gcaaactcaa gggcagtcgc ctcttgttct gttggctggc ggtctgtcac cacttgccag
     2941 tcagatggat tttcagccac aacgtcttgg ttttgcacca aaagtccacc aactacgcca
     3001 gtgtactcag cttccacttc gctagcagct tgggcatcaa acggcaactc aaggatacgc
     3061 aagtttttct ttttatttgt gagaatagct agcgcttctt ctgagtatga tggtgcgatg
     3121 atgatttcca agaagatagg gtgcatcttc ccagctgtcg ctgcgtcaac ttcacggtta
     3181 aggacaacaa ttccaccaaa gattgaaact gggtcagctt cataagcgta atcccaagct
     3241 gtctcaatat catcagcctg tccgatacca catgggttca tgtgtttgag ggcaacaacc
     3301 gttggactgg ctttgaaatc gcggataata cggatcgccg catcagcatc acggatatta
     3361 ttgaaggaca attctttacc gttgagctgt ttagctgaag cgattgagta gtctgttggc
     3421 aaggcttttt ggtagaaatc agcgtcttgc tgtggatttt ctccgtaacg catagcctgt
     3481 ttaaggtcat aagtgatggt caatttttca ggcttagcct ctcccacttg agctgtgaag
     3541 tactcagcaa tcaaagcgtc gtaggctgcc gtatgacgga aggccttagc tgccaagcgt
     3601 tgacgagttt taaatgtcgt ctgactagcg tcagccaatt ctcccaaaac agtggcatag
     3661 tcagctggat caaccacaac ggttacgcta gcgtggtttt tagcagctga gcgaagcatt
     3721 gatggaccgc cgatgtcgat attttccacc gccaaatcgt aggtcacgtc tgggcgaagg
     3781 atggtctcct tgaagggata gaggttgaca accacgaggt caatcagctc gatattattg
     3841 tccttagcag cctgaaggtg gctgtcagcg tcgcgacgag ccagaagccc accgtgaatg
     3901 tttgggtgga gggtcttaac acgaccgtcc atcatttctg ggaatccagt cacatcgtcg
     3961 atggcaatgg tctcaacacc agcatcatct agggaaacct tagtcccacc agttgagata
     4021 atatcccaac ccaagttttt taattctttt gcaaagtcaa caattcctga cttatcagaa
     4081 actgagatta aagcacgttt agtcatgagt tctccttttc ttacttcaat ctatttcata
     4141 ggctatgtat tcatgagaaa attcataacc gagtttttca gctaaattta atgaagtcct
     4201 tgtatgagca tcccagctag gataaattcc cttgtctaaa caagttagta tcaactgagc
     4261 tgcaactatt gttgctaaac cacgccgacg aaaatctgga tgcgtatcta cttctatctc
     4321 aatcccattt ttataggttg aataagatga agctcctgca atgatattcc cctgataata
     4381 tacaacataa cctatacctt gttttttata gtactgataa gtagcgtaat ttgctactaa
     4441 atcctgtgac cattcctttt ctaagcaaga gttgtaaact ttctcatcaa tagcacgtaa
     4501 ttcaaaacca tttggcaatt gagtaacaaa cttctctaac ctacttcgct caaacaaagt
     4561 atcttttttc gtcgcataac gcttaaaaga atgagcattc tgaccataag ttgattcaat
     4621 caaatctgac catcctttat gctgaggaac aaggataata tcctccccag agcaaacttc
     4681 tagtagaaat aaagtaggct gacctgctag aaaaccaaaa gatgatttcc ttcccaattt
     4741 tgccaaagaa gattttggtt ggtctaaact atctacaaaa acttctccca taataccttg
     4801 gacacacgac cagattatgg tctcgtccca atcaccaaaa atagactcta ctatctctaa
     4861 atttttcact agtttcatta gcttattata acaaatttta ctgttttctc tttatgccca
     4921 ggctatctaa gacagctggg tagagttggt attcggtttc gtggatgcgg gtttcaaagc
     4981 tttctagact atcatctgct aggcgtggca cgcgcacttg ttggatgacc tgaccagtat
     5041 ccacaccaga gtccacccag tggatggtca caccagactg gtcaacacct gcctcccaag
     5101 catcctcgat accgtgggca cctggaaatt caggcaagta ggctgggtga atattgatga
     5161 tacgcccttc ataggctgag agcaaggctt ctccgacaat cttcatgtag cccgccagac
     5221 agaccaagtc aatttcgtgt ttgtccaaca aattaacgat ggcttgctcg taagccgcct
     5281 tgttctcaaa ctccttgagt tcaaaagcga aacttggaat ggttaagttc tgagcacgct
     5341 ctaaaacata ggcatcacga tgatctgaaa agacaaaact aactggaaac tgctctgcta
     5401 tgatctgaaa gttggaacca ttaccagaag caaaaacagc gattttcata ttcgatacaa
     5461 agggatgatt cggcttgccg aaaaatttcg tagaaaaata ggaaaacgaa caacgacact
     5521 tgcgtctaag tcgttttatc ttttttccta gaaatttaat cccgtgttca gttaggcaaa
     5581 ctaactaaac actcctttct tataaaattt agtgacttat ttaatcacca cactatcgtc
     5641 tgctttcttg atgatacgac cgatttcata aactggttcg tccaagagct ctttgacgcg
     5701 gttaacattt tcaggactaa cagctagcat aagaccgaca cccatattga agatttcaaa
     5761 catttcttcg tgcttgatat caccatattt ttcaatcgca ttgaaaatcg gaagtactgg
     5821 caccttatcc tcatcgattt ccgcagccaa atcatccgca aacatacgag gaacattctc
     5881 gataaaacca ccacccgtaa tgtgggcgat accgttgact agctcttcct tgatcaatgg
     5941 cagaactgct ttaacataga tacgagttgg ctcaagaaga acatccttga gttgtttgcc
     6001 ttcaagctct ggaagcacct cattaccagt gtagtcagca aagacacgac gtaccaatga
     6061 gtaaccattc gagtggatac cacttgaagc aagtccaaga agaatatctc cctcttttac
     6121 ttttgaaccg tcgatgattt gagatttttc agccacacca acagcaaagc ctgcaaggtc
     6181 ataatcatct tcgccataca taccaggcat ctcagccgtt tcaccaccaa taagagctgc
     6241 gccagcttga acacaacctt cagcaacgcc agcgacaacc tgttccaatt tggcaggttc
     6301 gtttttacca gtcgctacat agtcaaggaa gtaaaggggt tcagcacctg ctgcaataat
     6361 atcgttgaca cacatggcaa cacagtcttg gccaatggtg tcatgctgtt gatatttaat
     6421 cgcaagcatg agttttgtgc caacaccgtc tgtccctgag accaaaacag gctctctaac
     6481 tcctgtttta ctcaagtcaa acatcccacc aaagccacct agagctccca tgactcccgc
     6541 acgttctgta cgagcaacgt gtttttttat tctttcaacc acttcataac cagcttcaac
     6601 atcaacacca gattttgcat aagcattttt ttcagacatg ttttctcctt atataattag
     6661 cagagcttat ttcacttttt gaatataaaa acttgttttt tcttctaaac ttctgaggta
     6721 ttcttcttca taatcataaa gcggtgttgg ataatgtcca tcaaagtaag ctacgcataa
     6781 accaccattt ggtgcttttg tttcgagtcc aattgattca atcaacccat ctagtgagag
     6841 ataggtcaga ctatctgcgc caataatatc acacacttcg tcaacagcgt gatttgctga
     6901 aattaattct cgtcttgttt gaatatcaat accataaaaa caaggatact ttaattctgg
     6961 actagctata gcaacatgta cttcactagc tcctgcttct cttaataatc cgacaatcct
     7021 tctagaagtc gttcctctta caattgaatc atcaatcata acaacgcgct ttcctttgac
     7081 aacaccagat accgctgata gtttcattcg aacaccttgt tcccttaatt cttgtgtcgg
     7141 ttgaataaag gttcgctgcg tatactgatt ttttacaaga cccatctcat ttggtagtcc
     7201 ggattcttca gcaaagccca tagcagccga taaggacgaa tttgggacac caattataat
     7261 atcagcatcc tgtttaaatt cttgtgcaag acgctttccc atattttttc gagccgtatg
     7321 aacgttaaca ccatgtatag ttgaatccgg ccttgcaaaa tagacatatt ccatcgaaca
     7381 aattgccagt tgcgtttcat cagtataacg atcacattga ataccactat cgtctataag
     7441 aataacctcg ccaggttcaa catctctaac ccattttgcg cctaccacct caaaagcaca
     7501 ggtctcactg gaaataaccc aggcaccatt ttgcatttgt ccaattgaca aaggacgaaa
     7561 ggcattaggg tcaagagcag caattaattt atcttctgtc atcagtagat aggcgaagcc
     7621 tccctttaca gtgcttaaag cttcttttac cttccccata aaacttgggt tatggcttcg
     7681 acgaatcaaa tgcatcaaaa tttcagtatc tgaggaggca ttgaaaattg caccttgctt
     7741 ttctaattct ttccttaagg aaatagcatt tgtcaaatta ccattatgac ataaagcaaa
     7801 ttgcccgtca tgaaatttat aaagaaaagg ctgaatattg cgaatatctg cagaacctgc
     7861 agtagcatac cgaacatgtc caatagccgc attcccagtt aaattatcta attcagattg
     7921 attcttaaaa acttcagaaa ggagcccaac atttcgataa ccatagagtt tcccattatc
     7981 attcgaaaca ataccagcac cttcttgacc gcgatgttga aggctatgaa gcccaaagta
     8041 agtgacttga gctgcctgag gatgccccca gataccaaag actccacatt cttcatttag
     8101 agattttact tcgtatgtca ttcttttttc ctaattcttt ttgtatctct tcttaagtag
     8161 ctttgatgat tttctcaatt ttggatctat cgtcaccaat gccaacatca aaatagaaaa
     8221 tctatcaagc tattttcaag ctaatcattg agtcttagcc tcaacggctc ccaaatacca
     8281 ctacgacaga aagatacagt taagctcgct tcgctcttgt ttccttatct gttttatgaa
     8341 aaaaattgaa aaaattattt tcctgtaaaa tattttactg cactagcaaa caaggcttgg
     8401 tctttgttac cagggatatt ttggaagagg ccgtcttccc agcgttctga gtgccccatc
     8461 ttaccgatga tttgaccatt cttgctggta atcccttcga tggcattgac agagccgttt
     8521 ggattgtatt tagaatccat agatggttgt ccgtcaaagt ccacatattg gctccagatt
     8581 tgaccattgt ctcttagctc tgcaaattca gaagcgctga caacaaattt accttcaccg
     8641 tgtgaaactg gaatggcatg aatatcgccg acctcaactc ctgccaacca tggtgagttg
     8701 gtatttgcga tacgagtctc aaccatcttt gcaacgtgct ggttagcatc gttatagaag
     8761 agagttggac ttgtctcacc agcttcctcg aagtttccgt atggaagaag acctgattta
     8821 acaagagctt ggaatccatt acagataccg atgataaagc cacctttttc gatgaagctg
     8881 tcaatagctg cgcggacctt ctcgttaagc aagatattga cgataaactt agcagaccca
     8941 tctggttcat ccgctgctga gaaacctcca gcaaagaaga tgatatttgc cttagcaata
     9001 ttagcgacca ttgtgtcaac tgattcagca atagcagcct cattcaaggt tacaaatgct
     9061 accaagttga cactagctcc aacctgttca aaagccttag ctgaatcata ttctgagttg
     9121 gtaccaggga agactggaat gtaaaccact ggtttttcaa ttgtttcctt agccttgatg
     9181 acggtgtctg ataccacagc aggaacttct tcaagagcgt ctgcctgttc gaattctgtt
     9241 ggataaacct cttccaattt accttcgaag gctgctagaa ggctagcgcc agcaaggtca
     9301 tttccattga cagtgactgt aaagtctgcc tgagtttgac cgattttcac agcgccagcg
     9361 atttcctcag ctgatgtaaa aacaaagcct ccgagttgag ctgtcaagct gctgtcaagc
     9421 tctgcaattt caacagaggc cccgatacgg ttaccaaaag tcatgagagc aagactttct
     9481 aggacaccac cgtatttaac agctgaagca gcagtaatct tatgttgagc ttgaatagtc
     9541 tcgaactggc taaagttagc cttgataagg tcaaaatcaa tatcttctga aatagcttga
     9601 cctggaatgt agtagatgtt ctcaccagcc gctttaaact caggagagag aaccctgcgg
     9661 ctatccgcgg tcgtcacacc gaaagctacc aaggttggtg gtactgtcaa gtcttcgaaa
     9721 gtaccagaca tagagtcctt accaccgatt gatggcaagc caagttgaat ctgagcctca
     9781 atagaaccaa gaagagctga tactggctga ccaaaacgct ctgcctgttt atccatacgc
     9841 ttgaaatact cttggtaaga gaagcgtgca cgagaccagt cagcacccgt tgctaccaag
     9901 cgagctgtcg cttcaataac agcataggca gcaccgtgat aaggtgacca ctctgcaata
     9961 taaggattat aaccttgagc aataacagag gctgttgtcg tcacaccatg ttgaactggc
    10021 aatttttgaa cagaactttc tgtcggtgtg atttggtagc gaccaccgat tgggtggtta
    10081 acggttgagc gaccaacaga tgagtcaaag atagtttgaa gacctttttg gctagcatgg
    10141 ttgaggtcag acaagacctt aagcgtatct gcttcaagtg tctctgcaga tgttgtgcgt
    10201 gcttctggaa ctgtcaagtc cttgtcaacg actttagcat caacaacgac acggacaccg
    10261 ttggtatcaa ggaaacggcg ttccaagtca acgatgattt cgccattcca agtcatgaca
    10321 agatttggtt tttcagtcac agtcgcaact acgactgcat cgatattttc cttgttacag
    10381 gctgcgatga aggcatccac atcacttgga cgaacaacga ctgacatacg ctcttgtgat
    10441 tctgagattg caatttcagt accattaaga ccttggtatt taagtggcac cttgtccaaa
    10501 tcgatttcaa gaccatcagc caattcaccg atggcaacac agacaccacc tgcaccgaag
    10561 tcatttgatt tcttgataag acgagtgaca ttgccatcac ggaaaagacg ttgaatctta
    10621 cgctcttcaa tggcattccc tttttgtacc tctgcgccag ctgtttccac agattcaacc
    10681 gtttgaacct tagatgaacc tgtcgcacca ccgacaccat cacgacctgt tttaccaccg
    10741 agcaagatga ccacatcgcc tgtttctggt ttttcacgaa ccacattttc cttaggtgca
    10801 gcaccaacca cagctccaag ctccatacgt ttggctacga agccagggtg gaagtactca
    10861 cgcacatacg ttgtcgcaag cccaatttgg ttaccatatg aagaatagcc gtgcgccgca
    10921 gtcttagaaa taacctgttg tggcaatttt ccagcacgtg tttccgcaat cggagtcgtg
    10981 atatcgcctg cgcctgaaat acgcatagcc tgataaacgt atgaacgtcc tgacaacggg
    11041 tcacgaatgg caccaccgat acaagttgct gcgccaccaa atggctcaat ttctgttggg
    11101 tgattgtgag tctcattctt aaacatgagg agccaaggct cttttacacc atcaacatct
    11161 acttcaattt caactgagca ggcattgatt tcatctgaga cttccatatc gtccagacga
    11221 ccgttggcac gctcataacg accaaaaata gtcgccatat ccataagtgt ctgcggcttt
    11281 tcagaacgat caagttcatc acgcatggcg atatatttgt cataagtcgc ctgcaattgt
    11341 ttttggaatt tagaagctga aaaatcaatg ttcttcaatt cagtttcaaa ggttgtgtga
    11401 cggcagtggt ctgaccagta agtgtccaaa actttcagct cagtctcagt tggcacacgc
    11461 ccgattgatt tgaaataatc ttggatgaag agaaggtcat cgacctccat agccaagccc
    11521 tgctctgcct tataggccac aaaatcgtca gctgtataag tctcgaagaa atcaagatta
    11581 gggatcgtct tatcagacac agagaaagcc tgctcttcaa gcggcaaagt aatgtcctta
    11641 aaacgtgaat caactggatt caaaagatag tttttaaccg cttcaagttc tgcttctgca
    11701 atatccttat tgaccaagta aagctgggcg gtgttgacct tgacctgact gtcacctcca
    11761 agcaatagca aagcttcttg cgaactagct gcacgttggt caaattgacc aggaagggcc
    11821 tcaatagcaa agaaggcaac cttatcaagc tccgcagtga tttcagcttc tgtcaaaaga
    11881 cggtctgtca cctgctcaga gaaaatatgc ttctccgcac gcgccagcaa atcctcagcc
    11941 aaatgaaaga catcatagac ctgcacaata cgcaactcct tcaaagaagt caattgcaaa
    12001 ttatgcgtca actctttcac aagactagcc gatttaatgc caaagtcagc ctttttctca
    12061 acaaaaatac gtttattcat cataaataat caagttcaac atcatttaga ttcaataata
    12121 aaagatcatc tgaaaatata ccccaaaatg aactaccttc tatcaatctt tgtgaacaaa
    12181 tatctccctt ccatatactt tcaatcacta ctattaatta agtgatttcg agattaatct
    12241 aaggtcttta atttatctaa gacaacttga taagcatcag ttaaactacc taaatccctg
    12301 cgaaagatat ctttatccat atgatttcct tcagcatccc atagacggca gttatcaggt
    12361 gagaactcat cagccaagat gatcttacca tcattgtcaa atccaaactc caacttgaag
    12421 tcgatcaagt taatcccaat ctgagcaaac caatctttca agagttcatt aatacggcga
    12481 gtctctcctt tcagataagc aatctcctca tcattaacaa tacccaaaaa cttcacatgc
    12541 tcatcattga taaaaggatc attcagatca tcatttttgt agtaaaattc cacgattgga
    12601 gtttcaagaa cgcgtccttc ttccactcca aaacgttttg aaaatgagcc tgctgtcaca
    12661 ttccgtaaca ccacctcaag tggaataata tcaacttttt tattcaattg ttcgtttttt
    12721 gaaatttgct caatataatg tgtcaccaca ccagccatat ttaatttctc aaaaataaga
    12781 gatgaaatct gattatttag tgctcccttg ccatcaatag tctccttacg agctccattc
    12841 aacatagtag cttggtcttt atagaccgtt ctaatcatat tttcatcttt tgtactataa
    12901 atatctttcg cttttcctgt ataaataagc tggttagtca cttgattatt cgcctttctc
    12961 ttattttaag agttattcta acacataaca ttatctaata caacaaaaac tttatttaaa
    13021 atcaaaaaaa agaagaaacg ttcgtatttt cttccttttt attattactt atgttcaatg
    13081 atataatcta atagttgttg gacgcttgac atatgttcga cagcctcatc tggaatctct
    13141 agatgaaaca catcttccaa attgataata aactctgtta aagcgataga gtcaattgct
    13201 aaatcatcat gtaacgatgt ctctggtgtg atatctaact gtgggtcacc aagttgctgc
    13261 cttaataatt ccagcatctt ttcaaaaact tcatctcttg acatgagttc actcctattc
    13321 atctgtaaag gcatcaacta attgatcaac tacttgagtt tccaacattg tacgaacttg
    13381 cttcaaagta gaataaacag ctttagaatc acttgatcca tggcatttta ctattggagc
    13441 ttttaaacca aaaagaaccg caccacccgc gcttgaatag tccatattat cttttagctg
    13501 atataggcta tcttttaata gaagtgctcc taatttagct ttcacgccac ctgatttaat
    13561 tgaagatttt aggcttccca taatactcat agctgtacct tccattgtct ttagaacagc
    13621 atttccagta aaaccatcag tgacaacaac atctgctaca ctagacatca aatcacgtgc
    13681 ttcaatattt ccaataaaat taatagaagg ctctgctgct aacagttcat aggcttcttt
    13741 atgaagagaa tcgccttttg tttcttccgt tccgttattt aagagaccta cacgaggaat
    13801 ttcaatacca cggacatttt ttgcataaaa cgaccctaaa atagcatatt gatgaagatg
    13861 agaagctgtg ttttctgcat tagctcctaa atcaagcata tcaaaaccca caccatctaa
    13921 cgtaggcata gttgacatta agcctggtcg atcaacacct tttatacgtc ctactacaaa
    13981 caaaccagca gctaataaag ctcctgtatt ccctgcagaa ataaaggcct gtgcaacacc
    14041 atctttgact gccttagctc ctaagaccat cgaagactgt tttttacgac gaacagcttt
    14101 agcaggctca tcatcagaat taattttttc ttcagtatga ataatactaa ctcgctccgt
    14161 tactgttaaa tatttctcaa tttttttctg atcgccatag agttgtactt caatatcact
    14221 aaaatctgaa atagcttgat taacaccctc tacaatagct ttgggagcgt aatctccacc
    14281 catagcatct acagcaattt tcttcatgaa tgaactcctc ataaaaactt agtggctata
    14341 ttatagcacg tttctttatt tttttcacaa gaaggcaata atagctaata tctaacagat
    14401 actctcactc tttccaaaga aaagtatgct aaacaaccta ctaatatcaa agaacatagt
    14461 actccccctt caaaaccaaa atccccacca ttccaaagag caaagctaga attttcagta
    14521 attctaaaaa gagaaacagg agaatacccc ccactaacct tattacctaa tacacatccc
    14581 atagtaaaat tccatgtagt atgaactcct gtaacaaaaa gaatattttt ggtcatatta
    14641 aaaagaatag aaaaaataat accaagtaaa aataaattaa aaagtgctat tgctgtgata
    14701 ccatagtttc taaagtggat aaaggaaaac aacatagaat tgcataaaac acctgcaaaa
    14761 ctacctttaa atttggtaac tgttgtcata acatatcctc taaataaaat ttcctctgtc
    14821 aagctttgga taaagtatgc aagaaataaa aagaaaaaag gtccaaaaga aaaggaattg
    14881 ctggaagaaa tttccacaaa acctgataac cagtggcaaa tataaataaa aaacatcagt
    14941 aaactaccag taataaatcc tgagatcgtt gttaaaatat tattatcttt agtaaatccc
    15001 aaggaagtaa aatccctttt ttggaaatag attaaaaata gtaaaatact tgccaaaata
    15061 ggaacaaata gtaaaagcgc aatgtataaa ttgaatttat aatcatcagg tagcaatttt
    15121 actacaaggc gtctacttac aatgatgccg ataacttgaa ttagaactgc accaataact
    15181 ggcacaaacc agttagcaaa aaagagttta ttggtaatgt ataatgtttt tttcatctca
    15241 aatatcctaa aaattaattc catattagtt tatcctaata ttaaaaaaaa gaacatgaca
    15301 ctaatgtcat gttcctcttt acaacttaat ctgatttcat aatacttccc caactagaca
    15361 aatcatcaat aaatttttta cttttaagat gaatccctac atattcatcg taaatcatgt
    15421 ccaaaaattg acgaatttta agtttcatct caggtttcac agaaattgtt tgtaaatcat
    15481 caaattgaat tgactgaaaa cgattgatta agtgcagcat attaggatct aggtggtttc
    15541 ttctctcgtc tttataataa tggtttggac ataataatcc agaatatttg tgtgaaaaat
    15601 caaaaggtaa tcctgcccta tgacaaaaaa cacaatcatg aaaatttaaa gcaacaccga
    15661 acctctctag tagttgtact tcaaaaatat tcgttagaat ctcataatct aaaccgtcct
    15721 caatcaattc taacgttttc tttaggaaaa taaataattg agcatccgct acattatcac
    15781 taatagccac atcagccaaa gaagtaatat aactcgcata tgacaactta aacaagtctg
    15841 aattaatttc ttgaaatgct aaaacttctt tataatcatc aatataagaa agaccattat
    15901 cattaatttt taaaataaaa tgagcaatag tcaaaggttg gagcacagca ttgaattttg
    15961 actttgaagc atgctttaca aaaaacatcc tttttccctc agtctccgta aagattttaa
    16021 ctaatttatc atcttcacga taattacgat tatacaaaac gagaccgtat gtttgactaa
    16081 ccctcatatg attccataaa aactttcaat ctagccattg ctgtttcaat aacttccata
    16141 ctagcagcat aagaaagacg tatatatcct tcaccatatt taccaaaagc cacccctgga
    16201 ataatagcta ctgcttgttg ataagcaaaa tcctgacaaa acttaaaaga atcctgtgga
    16261 taactatcag gtattttagc aaagatataa aaagcgccac tcggtttaat tattttgaaa
    16321 ccaagtttag acattttatc aatgatataa tctctacgtt taatatattc ttgacgcata
    16381 ggtaacgtat catttttacc cacagataag gcttctaccc cagcaaattg cgaaatagta
    16441 ctagcagctg tcaccatgta ttgatgactt ttaatgattt gagcagtaaa tgcttcagga
    16501 gcatacacca atccaactct ccaaccagtc atagcatgtg attttgataa gccattaatt
    16561 aagatagttt ggttaggtaa atattctgca attgaaacat gctgctgacc agtgtaagtt
    16621 aactcagagt aaacttcatc actaataaca aaaatatcat attttttaag gacttcagct
    16681 aatgcagcaa tttcttgcct tgaataggta atacctgtag gattagtagg ataattaagt
    16741 aaaacagcct ttaatttttc accttgttgg ataatagccg tttctaacat ttcaggggtt
    16801 agtctaaagt cattttctct agtatcaatt tctacgatat cagctcctac aaggttaact
    16861 attggctcat agcctggata agcgggggca ggtaatagaa caacatcacc agcttctaaa
    16921 atagctataa gagatgcaga cagagcttct gtggcaccta tcgtaaccaa gatctcacta
    16981 tcaggattat aagtaagatt atatttatcc tttgcaaaat ctgcagcagc ttgccttaga
    17041 gctaataaac cgctcatacc agtgtaatac gattgattcg catcaatagc tgatttagct
    17101 gcttctttaa catgatcagg agtggtaaaa tctggttctc caagagttaa ttttaccatt
    17161 ccaggtatat ctgaaataga ttgatcaaac tgacgaatta aggagacctc tattctatct
    17221 aagtacttat taaatcgttt ctctaatgtc atca
//
LOCUS       DATRSD010000046        17049 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRSD010000046 DATRSD010000000
VERSION     DATRSD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17049
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.046"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(79..149)
                     /locus_tag="VBD85_001243"
     tRNA            complement(79..149)
                     /locus_tag="VBD85_001243"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Cys,seq:gca)
     gene            complement(284..1816)
                     /gene="ahpF"
                     /locus_tag="VBD85_001244"
     CDS             complement(284..1816)
                     /gene="ahpF"
                     /locus_tag="VBD85_001244"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO7133800.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGHESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(1834..2394)
                     /gene="ahpC"
                     /locus_tag="VBD85_001245"
     CDS             complement(1834..2394)
                     /gene="ahpC"
                     /locus_tag="VBD85_001245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO7133801.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            2671..3441
                     /gene="rpsB"
                     /locus_tag="VBD85_001246"
     CDS             2671..3441
                     /gene="rpsB"
                     /locus_tag="VBD85_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO7133802.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSKND"
     gene            3535..4575
                     /locus_tag="VBD85_001247"
     CDS             3535..4575
                     /locus_tag="VBD85_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO7133803.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(4735..5196)
                     /locus_tag="VBD85_001248"
     CDS             complement(4735..5196)
                     /locus_tag="VBD85_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO7133804.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            5369..5833
                     /locus_tag="VBD85_001249"
     CDS             5369..5833
                     /locus_tag="VBD85_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO7133805.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            5830..8277
                     /locus_tag="VBD85_001250"
     CDS             5830..8277
                     /locus_tag="VBD85_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7133806.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITDYQFLEQSSALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDTE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(8497..8988)
                     /locus_tag="VBD85_001251"
     CDS             complement(8497..8988)
                     /locus_tag="VBD85_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO7133807.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARAAYDWTCELSIYLDKDARSKKISSQLYDT
                     LEKELVSQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(9002..9643)
                     /locus_tag="VBD85_001252"
     CDS             complement(9002..9643)
                     /locus_tag="VBD85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7133808.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNYKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSKYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(9764..10741)
                     /gene="dusB"
                     /locus_tag="VBD85_001253"
     CDS             complement(9764..10741)
                     /gene="dusB"
                     /locus_tag="VBD85_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO7133809.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQSNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(10725..11600)
                     /gene="hslO"
                     /locus_tag="VBD85_001254"
     CDS             complement(10725..11600)
                     /gene="hslO"
                     /locus_tag="VBD85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO7133810.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMENGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(11740..12996)
                     /locus_tag="VBD85_001255"
     CDS             complement(11740..12996)
                     /locus_tag="VBD85_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO7133811.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(12993..13811)
                     /locus_tag="VBD85_001256"
     CDS             complement(12993..13811)
                     /locus_tag="VBD85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133812.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKRFTSNQRIEALKSNIKETDQDIRHLEILKKDNLSKEYIKLANQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEKADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(13935..16187)
                     /gene="gshAB"
                     /locus_tag="VBD85_001257"
     CDS             complement(13935..16187)
                     /gene="gshAB"
                     /locus_tag="VBD85_001257"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO7133813.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKACRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPHYQDLLESVKRQIQSPELTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            complement(16357..17031)
                     /locus_tag="VBD85_001258"
     CDS             complement(16357..17031)
                     /locus_tag="VBD85_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7133814.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILLKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
BASE COUNT         5312 a         3001 c         3200 g         5536 t
ORIGIN      
        1 aaaataattc tgataacaaa aaaatcaacg aacaatcgtt gattttttgt tatctttgga
       61 taaaatcttt tatatttaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga
      121 gccgtgcctt accacttggc tataccgcca taacagataa tattctatcg aaaaaaaaaa
      181 gattcgtcaa gcctctttcg taaaatagcc aattttagcc cttaaacttc ctcatattac
      241 cccaaaaaag agattgatac ctattgtatc aatctctttt agattattgt ctgattaaat
      301 aatcaaatgc gccaatggca gctgtggcac cagaccccat ggaaataatg atttgcttgt
      361 aggcagcatc tgtacagtca cctgcagcaa aaataccagg gatatttgtt gagccaaatt
      421 tatcaacgac aatttcttga cgttcattga gttcaatgcc gctatcctta agccaagatg
      481 tacttggtac aagaccaatt tgtacaaaaa caccttctaa atcaatgtgt ttttcttcgt
      541 ttgtatcacg atcggtatag tttaaaccag tgacatgatc ttcaccaacg atatctttag
      601 tagcaacatt cttaagaata gttaaattat cagttttagc tgctcgttct tgcagtactt
      661 ggtctgcttt gagctcaggt aagaattcta ggactgtaac atgtttagtg acaccggcta
      721 gatctaaagc agcttccata cctgaattac caccacctat aacagcaaca tcttttcctt
      781 cgaaaagagg gccatcacaa tgtggacagt aagtaacacc tttattgcgg aattcttcct
      841 cgccaggaac gttgatattt cgccacttag cacccagagc taggatagca gttttagctt
      901 gtaaaatagc tccgttagcc agtgttacct caactaattc ttttttttca atgccagttg
      961 ctaattgact cttgataata tcaatatcgt aagatttggt atgctcttca atttgagcca
     1021 tcaatttagg gccttcggtg tacaatgtac caatcatgtt ctcaatacca actgtttcga
     1081 taacttgtcc accaaaagtt tctgctaaaa tacctgtttt taacccttta cgggcggcat
     1141 aaattgcagc actgttacct gcaggaccac caccaataac tagaacatca tagacacctt
     1201 tgtcagcaaa tgcttcagca tcaagaggtc catctagttg ttctaaaagc tgttcaattg
     1261 tagcacggcc agaagtaaat tcttcttggt ccttataaac agtaggaaca gacatgatac
     1321 cttttgattt gacttcgtct tgatacatgc ctccttcaat catagtatga gtgatattcg
     1381 gattcaaaac agccattata ttaaaggctt gaacgacatc tggacaatta tggcaagtta
     1441 atgaaacata agtttctaga ttgatagttt tctcaatgcc cttaattcgt ttgataatgt
     1501 cttcatcaac ttttggtgcg cgaccagaga cttgcaacaa agctaaaatg aaagatgtga
     1561 attcatgccc cattggcaag cctgaaaaaa taacgcgact ttcatgccct ttttttgcaa
     1621 tcccaaaact aggttgacgt tttaaatgtg ttgattcaag gctgatgcga tcagacatag
     1681 cgacaatttc atctagaaaa tcctttactt tttgagaatt atcgttatct cctaaatcag
     1741 cttgtaatac aatatcagat tctagcaaat ctaaatattg tgctaattga gccttgattt
     1801 ctttatctaa gaccatggat tacctcctgg ttattaaatt ttacctacaa ggtctaaact
     1861 aggagttaaa gtttcagctc cttctttcca tttagctgga caaacttcac ctggatgttg
     1921 gcggatgtat tgagcagcac ggactttatc aatgagagta cttgcatcac gtccgatgcc
     1981 atcagcatta atttccatca tttggatgac accgtctgga tcaatgataa atgtaccacg
     2041 ttgtgctaga ccatcttgtc caaggacatc aaaaccttgt gaaataaggt gtgaagggtc
     2101 accaatcata ggatatgtga tagtaccaac gacatctgag tcatcatgcc atgctttatg
     2161 aacaaaatga gtatcagtag agacagaata aacttctaca tcaagagatt taagtgtttc
     2221 gtattgctct tgaaggtcac caagttcagt tgggcaaaca aatgaaaagt ctgctgggta
     2281 gaaacaaaaa actgcccatt ttcctttaac gtcttcattt gtaacagtga tgaattttcc
     2341 atcgtgataa gcttgagctg aaaattcaat aatttctttt ccgactaatg acataataat
     2401 gtcctccttt attttttaat agcctaatta taaacgaaat acttttaaat ctccaataaa
     2461 aacgtttaca tttttctaag aaaagtatga tttttctaat agagtagaag attttatagc
     2521 ttgtcaaatc taactttttt tgataaacta ttatagttgc ttaaagtgac aaatactcat
     2581 cttagatcaa ttgtggtctt gttgccgaaa ggttaggctg caagtcgaag tctgagagag
     2641 gaaaaaacat ttttaaagga gaacttactc atggcagtaa tttcaatgaa acaacttctt
     2701 gaggctggtg ttcactttgg tcaccaaact cgtcgctgga accctaagat ggctaagtac
     2761 atcttcacag aacgtaatgg tatccacgtt atcgaccttc aacaaactgt aaaattagct
     2821 gaccaagctt acgaatttgt tcgtgatgct gcagcaaacg atgcagttat cttgtttgtt
     2881 ggtactaaga aacaagcagc tgaagctgtt gctgaagaag ctaaacgtgc tggtcaatac
     2941 ttcattaacc accgttggtt aggtggaact cttactaact ggggaactat ccaaaaacgt
     3001 attgctcgtt tgaaagaaat caaacgtatg gaagaagaag gaacttttga acttcttcct
     3061 aagaaagaag ttgcattgtt aaacaaacaa cgtgcacgtc ttgaaaaatt cttgggtggt
     3121 atcgaagata tgccacgcat cccagatgta atgtatgttg ttgacccaca taaagagcaa
     3181 atcgctgtta aagaagctaa aaagcttggt attccagttg tagctatggt tgacactaac
     3241 gctgatccag atgatatcga tgttatcatc ccagcaaacg atgacgctat tcgcgccgtt
     3301 aaattaatta cttctaaatt agctgatgca gttattgaag gtcgtcaagg tgaagatgct
     3361 gacgttgact tcgctcaaga agcacaagca gattcaattg aagaaatcgt tgaagtagtt
     3421 gaaggatcaa aaaacgacta atatttaatt tattgtaaat cctaaaacgg ggcagatgca
     3481 aaccgctctg tctcgttttt ttaatcatta tttgaaatta agaggagaac gaaaatggca
     3541 gaaatcacag ctaaacttgt aaaagaattg cgtgaaaaat caggtgcagg cgttatggac
     3601 gctaaaaaag cattagtaga aactgatggt gaccttgata aagcgattga attacttcgc
     3661 gaaaaaggta tggctaaagc agctaaaaaa gcagaccgtg ttgctgctga aggtttaaca
     3721 ggtgtttatg ttgatggtaa cgttgcagca gttattgaag ttaacgctga gactgacttt
     3781 gttgctaaaa acgaccaatt tgtaacatta gttaatgaaa ctgctaaagt tattgcagaa
     3841 ggacgtcctt caaacaatga agaagcactt gctttaacaa tgccttcagg tgaaacttta
     3901 gaacaagcat ttgtaactgc aactgctaca attggtgaaa aaatttcatt ccgtcgcttc
     3961 gctttagttg aaaagactga tgagcaacat tttggtgcat accaacataa tggtggacgt
     4021 atcggagtaa ttactgttgt tgaaggtggc gacgatgcac ttgctaaaca agtatcaatg
     4081 cacgttgcag ctatgaagcc aactgttctt tcatatactg aacttgatgc acaatttgtt
     4141 catgatgagt tagctcagtt gaaccacaaa attgagcaag acaacgagag tcgtgctatg
     4201 gttaacaaac cagctcttcc attccttaaa tatggatcaa aagctcaatt gactgatgaa
     4261 gttattgccc aagcagaaga agacatcaaa gcagaattag ctgcagaagg taaaccagaa
     4321 aaaatctggg ataaaatcgt tccaggtaaa atggatcgtt tcatgcttga taacactaaa
     4381 gttgaccaag agtacacact ccttgctcaa gtttacatca tggatgatag caaaacagtt
     4441 gaagcttatc ttgagtcagt aaatgctaaa gcagttgctt tcgtacgttt tgaagttgga
     4501 gaaggtattg agaaagcttc taacgacttt gaagcagaag ttgcagctac aatggcagca
     4561 gcacttgaaa aataattaac actcgcttta tattataatt tggcagcaaa atgttgtcag
     4621 taaatatatt ttaaaataag acacttggat taagtcaagt gtcttatttt ggtatcgaat
     4681 tggtggattg gcaaatatta aaaaaggaga gacagtcatc tcaccttaac ttttctagaa
     4741 taggttgaag ccgataatgg ataatgttcc catgatgcat ccagctagta taacacctag
     4801 cataacagca ataacactat gtttaaattc ccaatctagg aggctagctg caagcgtgcc
     4861 agtccaggct cctgttccgg gtaagggaat gcctacaaac aatagcaagg caataaacaa
     4921 tcctttttct ccagctactt tttcaagttt ttgaccaccg ctatgtcctt ttttaaggca
     4981 ccatgtgaaa aacttaccag tataaggttt atctgctccc cattctagaa ctttgcgagc
     5041 aaagaagaag atgataggta caggtagcat gtttccaaca acaccgatag ctagcgcttc
     5101 ccacaaaggt attccattag cgattgcaaa tggcacagct cctcgtagtt ccactaaggg
     5161 aatcattgaa atcaaaaatg taataatgta attcattaaa taaaaccttt ctatggtatt
     5221 attccatttt atcttatctt gctagtaatg acaactaaaa tccgcggtca ttctagtttt
     5281 actatcctta gatagatttc atttccgtat cttttaaaaa tttgatataa taatagtcag
     5341 ttatagtcaa agaggaagga gtgaatttgt ggccataaaa aatacttccg ataatattga
     5401 agaatatata aaatcattat tagaacaatc aggaattgct gaaataaagc gttccaattt
     5461 agctgatact tttcaagttg ttcctagtca gattaattat gtcattaaga cacggtttac
     5521 tgaaagccgt ggttatgtgg ttgagagtaa acgaggtggt ggaggttaca tccgcattgc
     5581 taaggttcat ttttctgatc aacaccaatt atttggaaat atgttatcaa cgattggcga
     5641 gcgtatcagt gaacaagtct ttgatgattt gattcaatta ttattcgatg aagagataat
     5701 tacagaacgt gaaggaaatt tgattctggc gacatcaggt gatgacgtct taggagagca
     5761 agcctctgtt attagagctc gtatgctccg taaattatta caacgattag atagaaaagg
     5821 attccattta tgagtcatta ttccataaaa ttacaagaag tattcagatt ggcgcaattt
     5881 caagcagcac gctatgaaag tcactattta gaaagctggc atcttctttt agcaatggtt
     5941 ttagttcatg attcagtagc tggtttgaca tttgctgaat atgagtctga agttgcaatt
     6001 gaagagtacg aagcagcaac aattttagct ttaggtagag caccgaaaga agagattacc
     6061 gattatcaat ttttagagca atcatcggct ttgaaaaaga tcttaaagtt agcagaaaat
     6121 attagtatcg tagtaggagc tgaagatgtg ggtactgaac atgtcttgct tgctatgttg
     6181 gttaataaag atttattagc cacacgtatc ttagaattag ttggttttag aggtcaagac
     6241 gatggtgaat cagttcgtat ggttgattta cgtaaggctc tagaacgtca tgccggtttt
     6301 acaaaagatg atatcaaagc aatttatgaa ttacgtaatc ctaaaaaagc aaagtcgggt
     6361 gcttcttttt ctgatatgat gaaaccaccg agtactgctg gtgatttagc tgatttcaca
     6421 cgtgatttat cacagatggc ggttgatggg gaaattgaac ctgttattgg gcgtgataca
     6481 gaaatttcac gtatggtcca agtcttaagt cgtaaaacaa agaataatcc tgtcttagtt
     6541 ggagatgcgg gtgtcggtaa aacagctttg gcgtacggtc tagcacaacg tattgctaat
     6601 ggaaatattc cttatgagct tagagatatg cgtgttctag agttagatat gatgagcgtt
     6661 gttgctggaa ctcgttttcg tggtgatttt gaggaacgta tgaatcaaat cattgcagat
     6721 attgaagaag acggtcatat tattcttttc attgatgaac tgcatactat tatgggttct
     6781 ggctctggta ttgatagcac acttgatgct gctaatattt taaaaccagc tttggcccga
     6841 ggcactttac gcactgttgg agcaacaact caagaggaat atcaaaagca tattgaaaaa
     6901 gatgctgctt tatctcgtcg ctttgcaaaa gttttagtag aagaaccaaa tcttgaagac
     6961 gcttatgaaa ttctacttgg attgaagcca gcgtatgaag cctttcataa tgttactatt
     7021 tcagatgaag cagttatgac tgctgtaaaa gtggctcatc gttatttgac aagtaagaat
     7081 ttaccagact cagctataga cttacttgat gaagcttctg ccactgtgca aatgatgatt
     7141 aagaaaaatg ctccttcgct tttaacagaa gttgaccaag ctattttaga tgatgatatg
     7201 aaatctgctt ctaaagctct aaaagcaagc cataaggata aaaagcgtaa gcctattgcc
     7261 gtaaccgaag atcatattat ggcaactctc agccgtctat ctggtattcc tgttgaaaaa
     7321 ttgacacaag ctgatagtaa aaaatacctc aatttagaaa aagaattgca taaacgtgtt
     7381 atcggtcaag atgatgcagt taccgccatt agtcgtgcta tccgtcgcaa tcaatctggc
     7441 attcgtactg gtaagcgtcc tattggttcc tttatgttct tgggaccaac tggtgttggt
     7501 aagacagagt tagcaaaagc cttggcagaa gtgttgtttg atgatgaatc agccctaatt
     7561 cgttttgata tgtcggaata tatggaaaaa ttcgcagctt ctcgcctgaa tggggcacct
     7621 ccaggttatg ttggttatga tgaaggagga gagctgactg aaaaagtacg caataaacca
     7681 tattcagttt tactttttga tgaagttgaa aaagctcatc cagatatttt taatgtatta
     7741 ttacaagtgc ttgatgatgg cgttttaaca gatagtcgag gacgaaaagt tgatttttca
     7801 aatactataa tcattatgac ttctaattta ggagcaactg cattacgtga cgataaaaca
     7861 gttggttttg gagctaagga tatcagccat gattatactg ctatgcaaaa acgtattatg
     7921 gaggagctta aaaaagctta tcgaccagaa tttatcaatc gtattgatga gaaagttgta
     7981 ttccatagtc tcagtcaaga taatatgcgt gaagttgtga aaattatggt taaaccttta
     8041 attttagcgc ttaaagataa aggtatggat ctgaaattcc agccttcagc cttgaaacac
     8101 ttggcagagg atggctatga tatagaaatg ggtgctcgtc cactgcgtag aactattcaa
     8161 acgcaagtag aggatcattt atcagaacta ctattggcaa atcaagtgaa agaggggcaa
     8221 gtaattaaga ttggcgtatc caaaggcaaa ttgaaatttg atattgcaaa atcataaaac
     8281 attccagtac cgatgggtac tggaatttta atataaaaag aaaatgtcca aaatattttg
     8341 gacattttct tatagattta tgaaaagtaa aaggatctat atctaacaat tgcacttgta
     8401 gatataggta cgactgaaag tcgttgaaaa attttaggat aaatatatta taatatccta
     8461 atcttttgct aacttaaaat aaatctaaag attaccttaa gatttcaatc gcttttgtaa
     8521 ccatacaatg tcatgccatt gttcaaattt aaatccgatt tttgggaaat gtgcgacttg
     8581 attaaatcct ctttttttgt ggaatgcaat acttatatca tttggtaaac tgatacaagc
     8641 gagtagatta acataccctt ggctaactaa ttctttctcc aatgtatcat atagttggct
     8701 actaattttt ttgctacgtg catctttatc aaggtaaata gaaagctcgc aagtccagtc
     8761 ataggcagct ctagcgtagt aagttgaagc atatgcataa cctaatattt tcccattttc
     8821 ttcagcaact aaatatggga aagatttgct gatatttttg atacgattgg tgaattccga
     8881 tatactagga atatcgtatt caaacgttat tgtagtgtca gtaacgtagg gagcatagat
     8941 agcaagtaat tctttagcat ctgagggtag gacaggtctt ataatcatag atatcaccta
     9001 attatagtaa atcaagtttt tgcaattcgg aatcaatagt gtcaaggacg tgttgaagat
     9061 cctctggatt tttgacgaaa tcaagtttgt taccatcgat ccgaatttta ggtgaaacgt
     9121 cgtaattttc ataccattct ggatatttag aatggacttg tttgtagtag tcgtataatt
     9181 ctgggttaga atcaacttgc tcaaagctgc gtcctcgttt gtcaatacgt tctaacattt
     9241 tatcaaatga aacatcaatg tagactagca aatcaggtct cttcttaggc ataccttcta
     9301 attcttccaa catatttgca agaagttctt tatagatatc tagctctgtt ttagtcacat
     9361 ttccattctt ataatttaga gtgaggaata gttcatcttc aaaaatagaa cgatccaaaa
     9421 cattgttgtt agccttatag gcttctttta tagattggaa tcgtttattt aaaaagaata
     9481 tttgtagtaa aaaagcatat tttttgggat cttgatagta aagatctaaa actgggttat
     9541 tatcaaccgc ctcatagaaa acatctgtcc ctaggtgttg tcctagagca gctgcaaggg
     9601 agcttttacc tgctccaatg gttccagcta aaacaatcaa cataattctc cttaattctg
     9661 ttatgctaaa ataatttgtt atacttttga gttttcatta cacaatgatt tatgctaaat
     9721 ttgtacagag ataggaaaaa acagtcaaag gactgttttt gatttagcgt aaacctgcaa
     9781 aaagttcttg aacttctgct agtgtttctg cacgtgaaac agctcctcta atttttgctg
     9841 cgcctgattt tccacgcaga taatgaggtg ctaaccctcg aaattcacga acggcaatag
     9901 tttcaccttt taaattaact agtcgagtga gatgatcctc ggcaacatct aacatcttct
     9961 caaatggtag gtcaggaagg atttcgcctg tttcaaagaa atgattaatt tgtgtaaaga
    10021 tgtaaggatt gctacgagca ccacgtccaa ccattatagc gtcagctcca atctcttcaa
    10081 tcataaattt ggcatcgtga acagtacgaa tgtcaccatt ggcaataaaa ggaatactag
    10141 taacagcttt agcgaccttg ccaagtgttt catggtcaca cgtgccagta tacatttgtt
    10201 cacgcgttcg accgtgcata gcaagtgcag atacaccagc agattcggca gcgagtgcat
    10261 tttctacagc attagatgag tcagaccatc ctgtacgcat ttttactgtt aatggaatgt
    10321 caagtacaga cgttacctct ttaacaatat gataaatttt ttcagggtca cgtagccatt
    10381 tagctccagc ttcgttttta acaactttgt taactggaca tcccatattg atgtctacga
    10441 tatctgcttt cgtattactt tggataaagt cagctgcacg ttttaagcct tctgcatcgc
    10501 caccaaatag ttgaattgac ataggatgtt cattttcatc gatatggagc atatgtaagg
    10561 tcttttcgtt gttataaagt aaccctttct cagaaatcat ttccataaca acgagaccag
    10621 caccaaattc tttagcgata gtacgaaaag cagaattagt aatacctgcc atcggtgcta
    10681 gtacggtacg atgaggaatt tcaaccttcc caatcataaa tgaactattt aattgagtca
    10741 ttaattatct tctccaaatc ttcttctgtg aaatagtaag tctggttgca aaattgacaa
    10801 gtaatttcaa cacctttatc ttcatccttc atggcttgca attctttagt tcctaaagtc
    10861 aatagagcgg cttcaaaacg ttcttttgaa cagtcacagt agaaggctaa agagtcttcc
    10921 gataagcgct tgtagtcgtc ttctccgtaa atagctgaaa ggagagattc tatatgattt
    10981 tcagactcta gcagacttga aatagaaggc atctcctgaa tgcgtttttc atagcgagaa
    11041 atttcttcgt cagaagcacc tgggagcacc tgaagcatga atccacctgc aactttaacc
    11101 ttatcctcgt catctaagag gacatttaaa ccgacggcag aaggtgtttg ctcactttcg
    11161 gttaaataat aagcaaaatc ttctccaatt tctcctgtaa ttagtggagt tgttgaagtg
    11221 taaggttgcc cagttccgta atcggtaata acgacaaagt gtccattctc catgaaaggt
    11281 ccaactaaga cctctccagt agctgttttc ttgatatcaa cacccgtatt ttggatgtaa
    11341 cctttaacat ttcctttagt atcagctaca gaaataatat gaccaaaaga gctatctcca
    11401 ataaccttga cagttacttt actatttcct ttttgatttg ctgctaaaat ttgatttgca
    11461 attaatgttc tcccaagagc tacagtagaa cttgaaagtg tctgatgttt ttcttgagct
    11521 gttcttacag tttctgtaca atcaaggaca taagcccgga aagaaccgga agttgaaatt
    11581 gattttataa ttttatccat aagtcatatt atagcataaa aggcttattt tgcattggag
    11641 atagtcagga aaatatagat gaaaacggta taaattaatg atataaaaac gatacggtgt
    11701 tttacagtat cgtttttatt tagtggatat gatagtaagt taagactcat caactttttc
    11761 ttgggtagat ttaccttcgt tgagtaaggc ttcaactatt tttttatcac gaattttgac
    11821 agaatcattg attgtttcag ccgatttaat aacagcagat tctaattggg cacgttcttt
    11881 acgtcctttg tctatggcag cgataatacc attattttga gccactaagc tttcagctaa
    11941 tgcagtgaca gatttaatag aaacagtggg gctttgtgcg gtcttctcta acatcggaat
    12001 cgcttcttta ctagtttcag ccagcatctg caatgctgca ttattagcgt tgacaatagc
    12061 atcagcagtg acaccggatt tgacagattg ttgcatcatg cctaactgag cgattgagag
    12121 tttcattgtt ggaatggtat ttcgacgtaa catgccaagt ttttgacgca tatctgacga
    12181 tactttgacc aagtttcgca tctgtggtgt tgttgcccat gcaacgtaga gacggctgac
    12241 atattcagta tgttgctgtt cgagggtatt gataacttca gtcattcgag ctaattggtt
    12301 acttttaatt tgatactcgg acgtttggct atctaatgct agaatttctt gttgtaagtg
    12361 gcttgcacga ttggcagcct cggcttgact cgattcaata aaagcaataa ctccaaccaa
    12421 attttcaata gatttagtat tatcttcaat gagcatttca gcagagacga tatttcttgc
    12481 caaagtatct tcttgtttga caacattcgc tgccatcata tccatttttt gctcgatgtt
    12541 ttgtgagtca aaataaaatt cctgtagcga ggtcttgctt tgtttgaata atttttgaat
    12601 caagtttggt tttttctcta attctgccgg agtagcatct ttatatttgg caataaatcc
    12661 atttagttcg cgattagcat tttttagtaa atcatcaact tgaggaattt gaattttttt
    12721 ctgctcagac aagatatgat taacagtggt attaacgcct tctactgcgg attgtccaaa
    12781 atcaaggagc gcattttggt caccgacaaa agtatctacc aaagctggtg ttttttcaga
    12841 gatagcagac ttttgtgctg gtgttagttt ttcaaaaaag gcaatttgcc cagtttggct
    12901 tgttgtctgg ttggaaataa tttctgttgt tttatctgtt ttagtgatag cattgtctgc
    12961 aatttggtca atatcaaaat taaaggtatc gctcatttat tgttcctttc gtcttgcatc
    13021 attcttaggc taacgtcaaa atctttcata tcactttcat tcaattggcg cagcgtttca
    13081 tctaaatcta aattaaattt tttaattgct tgtaatgctt gttctaggcg ttccttagca
    13141 ttgttaaaat ttttaggaga ttgtttgatt ctgatgtagc cagccagaac atctgtaaaa
    13201 cgtttcatat tggcttgatg aattgctgct aattcagctt tgttatctgc cttttcaatc
    13261 ttttttaaga tagcatagtg gtcatcttga atattgtgat agaacggttt taattccgga
    13321 gcaatgcgta tcatttcatc ttcctgatca gaaataggtt ctagttctgg agaggcatgt
    13381 agctttatta gttgttcgtt aatctcatta ctaaatgtat ttgctttttt ggtgatacgt
    13441 ttatagatat taggctcaat ggctttttgt agttggtttg cttcattacg aattaggtcc
    13501 aattgaggta atatttgatt tgctaatttt atatattcct tagaaagatt gtcttttttt
    13561 aaaatttcca agtgtctaat gtcttggtca gtctctttaa tattggattt taaagcttca
    13621 atacgttggt ttgatgtaaa acgtttaaga cgctttttat cataaacgta ataggcacaa
    13681 taaccaccac caataattaa cagagtagca atcacctcac tccaaaagtc tgaaatcagg
    13741 gaagcaagaa caacaatacc aagtaatgtt attagattat gagaattttt ggaatagcgt
    13801 tttttaacca taataatatt taacctaact ttctgtagtt gttaatattt tatcttaaat
    13861 gaactagtga tgacaaggga taactttatt ttaaccaaaa attccttaag cttagcctaa
    13921 ggaactcttt ttagttataa ttctgggaac agtttagcca aaattcgagg tgtaatagat
    13981 tgaccaggcc cctcttgaca gtaggtgtgc atatacatca atgggttaaa gtttagttca
    14041 atgcaagtag catttttctt atcttttgag tatgcttgag tggcattagg aataattaaa
    14101 tcaactccac aaacccaagc ccccatagcc tctgccatct cagctgctag ttgtttatat
    14161 gtaggatcca ttgtatttgt tacatctata gagtcaccgc cagtggaaat attagaatta
    14221 cggcgaagtt ctatttttgt tccttctgga gggatactgt taacagtgta tccttgttgt
    14281 tccaacatta actgttctac ttctccgagc tcaataacct ctagaggaga gcgatgatca
    14341 tagcctctga gagggttttg gtttttaagt ttaactagct gactaatagt atgaatacca
    14401 tctccaacga cattagctgc taccctaagc aatacagcaa tacagtcccc ctcaaggacg
    14461 aagaaacggt attcggtacc ctcaatatat tcttccacaa gaatagcact atcttcggcg
    14521 aaagcgatgt caattgcttt ttcataagaa gctaagttag ctgatgtttt aaaaatagaa
    14581 atgcctaaac caaagtttgt tgattttggc ttaacgacga ttggtttatc ttggatttgt
    14641 gaaaaatagt taagtgcctc tttacggtca gtaaattcat ctccaaaagg agttgggaaa
    14701 tgcttttcat ctaggatttt ttttgtaaca actttgttag ccatagcgag cggtacaata
    14761 tagttatctt ttgaggtcat attaccattt ttaacgtact caatatgact attgtgccat
    14821 aattttagga attgatcttg ttcatccagc acttcgaagt tgactccttt ttggataacg
    14881 tcaaatagta gcaattgggt agaaagttcc atcgtttcat atccctttaa ggcgtaggga
    14941 gcttcccaag cataatcgtg ataaatttgt ccttgtcttt gtccaaatgt ttctaaggaa
    15001 agtccttcaa tcatttctaa aagttgacca gctaccgtta attcaggtga ctgaatttgc
    15061 ctttttacag attccaatag gtcttgatag tgaggtggta agttaaaatg ttggataaca
    15121 gattgcatag catccactaa gtcagaaact ggtgcttgat taggtaattt ttctaatgga
    15181 tgactgagtg ctataaggtc atttaatcta ttggcttcct tgatatcttg gtcaatatga
    15241 ctactagaat ctatccacaa aagcgccaat aagaaaaggt gaacagtatc aacagtttcc
    15301 tgcgttatac cgattggact aaatgggttg agatcaaaag tgcgaaattc taaatatgta
    15361 attccttttt ctaaataatt acgacaggcc ttactaccac ggagacgaac aggtgaataa
    15421 aattctttct cagcaatcaa ttgcccactt tttacagcat tttctagatc gttgacataa
    15481 tccttcaagc tagtataaga aatacgaata tctttatgat tcacataacc taagtgacta
    15541 tttctaagtg accgaacagg gttattcagt ttctgattta aaaagtcttc ttcagctacc
    15601 gggctcgctc catatagata agttagtaac caacggtaac gtaaaaaatt ttgagacaat
    15661 ttcatgtaaa gctggttctg aaaatcaata gcattgtctg cttgactcaa ttcaaagaga
    15721 gaagttaata actcttgacc taaaccgaga ttatagtgaa ttccagagat ggattgtatt
    15781 agttttccat aagttttctc caaatatttg cgataatcat attcaaaagc atcctctaat
    15841 tgggcaatct gtatgtcttc ttccctcact ttgggtggca tagaaagcgg ccataagtat
    15901 tcatcgtggt tgatagagcg tccagcaaca tcacttatag ctttcaaaaa tcgaatggct
    15961 tcttggctat cctttgcaat aggtgtaatg agttctaatt gaggttcact ataatccgtt
    16021 tggatataag gatgatagtt acgactccct aatgttttgg gatggggtgt ttgagcaacc
    16081 ctttgagtgg gttggtgaat gcgaaggctt tctctctcta agccaaatgt agcttgtaga
    16141 atcggtagat gagagtggct tctttgtaac agttgatcga taatcacgac aacattcctc
    16201 ctagtaacag atgatactag tttatcaaaa aaataataaa aactaagttt tacggcacag
    16261 aaaactaaaa taatcttata ccggtgtcat aaaaaataaa agattgaact tttgagtcca
    16321 atcttattaa tctataagtt aaaaagagtt ttagagttaa tgagtttcaa tttttttaag
    16381 ataacgatag acgcttggtt cggagatgtt gagaacttca gcgacctttg aaacggcacc
    16441 ttttaattgg aatacacctt tttcgtgaag tttagaaaca atttctacct taacttcttg
    16501 acttaaatga atattctgat ttaaaagact aggatcaaca atctcactaa taatatcctg
    16561 aatattgcct gatagaactt ctacaggttc ctcctgttga gggagagaaa ttttttgagg
    16621 ggacttaagt agtattttgt taacatttaa attaactaag tccagaatat ccaaagcaat
    16681 attttggtat gcagaaatat caaggttgat acataacatc ccttcaagtt cattttgagc
    16741 attcttgatg aagaaagtag atccacgaac ttctttattg agagggctga caatcgcctt
    16801 atagttagta acaaaatctt tttccttata aactttattc ttgattaaat caagggcgaa
    16861 agtcgttaga ggtgaactta tggttcgtcc actaatgtga ctattaacca actcaccaat
    16921 atagatatca ttttcatcta ctacatgcaa aacaatttcg taattatcac ctaaaacatt
    16981 atggagaaac gttattatcg tcttccaata gtctaatttt tctttatcca tatcgtagtt
    17041 cctctatgc
//
LOCUS       DATRSD010000047        17036 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRSD010000047 DATRSD010000000
VERSION     DATRSD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.047"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            2..4096
                     /gene="cas9"
                     /locus_tag="VBD85_001259"
     CDS             2..4096
                     /gene="cas9"
                     /locus_tag="VBD85_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286032.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO7133815.1"
                     /translation="MGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGA
                     LLFDGGNTAADRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLEDSFLVE
                     EDKRGSKYPIFATLQEEKDYHEKFSTIYHLRKELADKKEKADLRLIYIALAHIIKFRG
                     HFLIEDDSFDVRNTDISKQYQDFLEIFNTTFENNDLLSQNVDVEAILTDKISKSAKKD
                     RILAQYPNQKSTGIFAEFLKLIVGNQADFKKYFNLEDKTPLQFAKDSYDEDLENLLGQ
                     IGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSDSMIQRYDEHREDLKQLKQFV
                     KASLPEKYQEIFADSSKDGYAGYIEGKTNQEAFYKYLSKLLTKQEDSENFLEKIKNED
                     FLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENQDRIEKILTFRIPYYIGPL
                     AREKSDFAWMTRKTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDFYLPEEKVLPKHSL
                     IYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLDFLAKEY
                     EEFRIVDVIGLDKENKAFNASLGTYHDLEKILDKDFLDNPDNESILEDIVQTLTLFED
                     REMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTILDYLIDD
                     GRSNRNFMQLINDDGLSFKSIISKAQAGSHSDNLKEVVGELAGSPAIKKGILQSLKIV
                     DELVKVMGYEPEQIVVEMARENQTTNQGRRNSRQRYKLLDDGVKNLASDLNGNILKEY
                     PTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIIPQAFIKDDSIDNRVL
                     VSSAKNRGKSDDVPSLEIVKDCKVFWKKLLDAKLMSQRKYDNLTKAERGGLTSDDKAR
                     FIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFRKEFGFY
                     KIREVNNYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSATEKLFF
                     YSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYPQNNIVK
                     KTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLVVADIKK
                     GKAQKLKTVTELLGITIMERSRFEKNPSAFLESKGYLNIRADKLIILPKYSLFELENG
                     RRRLLASAGELQKGNELALPTQFMKFLYLASRYNESKGKPEEIEKKQEFVNQHVSYFD
                     DILQLINDFSKRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSLGAPAAF
                     KFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4098..4967
                     /gene="cas1"
                     /locus_tag="VBD85_001260"
     CDS             4098..4967
                     /gene="cas1"
                     /locus_tag="VBD85_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7133816.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARHDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLEPFDPSNREGHAARIYFNTL
                     FGNDFSREQDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYNGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            4964..5305
                     /gene="cas2"
                     /locus_tag="VBD85_001261"
     CDS             4964..5305
                     /gene="cas2"
                     /locus_tag="VBD85_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7133817.1"
                     /translation="MSYRYMRMILMFDMPTETAEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANSDSR
                     LVFLGDSYDQD"
     gene            5292..5957
                     /gene="csn2"
                     /locus_tag="VBD85_001262"
     CDS             5292..5957
                     /gene="csn2"
                     /locus_tag="VBD85_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO7133818.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMLVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6058..6620
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6058..6093
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            7178..7594
                     /gene="ndk"
                     /locus_tag="VBD85_001263"
     CDS             7178..7594
                     /gene="ndk"
                     /locus_tag="VBD85_001263"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO7133819.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRYADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESATREIAIWFNN"
     gene            7730..9562
                     /gene="lepA"
                     /locus_tag="VBD85_001264"
     CDS             7730..9562
                     /gene="lepA"
                     /locus_tag="VBD85_001264"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO7133820.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            9823..12441
                     /locus_tag="VBD85_001265"
     CDS             9823..12441
                     /locus_tag="VBD85_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7133821.1"
                     /translation="MIALAGLVLSCHFALTACHTQEHKNSHHIKTKQVAKKKANKKKV
                     SVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYSDLKGS
                     KFSYLIPNANTKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVVRHGDH
                     FHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSNGFLFNGRGIK
                     GTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKPRTENT
                     LPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDLSKPIP
                     NPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDEDQKKLIYLAEKLGLNPNQIEVL
                     TSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQIGFDDE
                     IIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPNIEVLG
                     IGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDLSFLTK
                     YKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHLDHNNV
                     KNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNPKVSNL
                     TINNAKLASLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTSLEGVN
                     NYTSLETLSVSKNKLESLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAVAKNFP
                     AVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPKTHDEH
                     HDHEETDHAHVGHHHH"
     gene            12541..13200
                     /locus_tag="VBD85_001266"
     CDS             12541..13200
                     /locus_tag="VBD85_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7133822.1"
                     /translation="MTMVKREDVILNATENMVHHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     R"
     gene            13209..13673
                     /locus_tag="VBD85_001267"
     CDS             13209..13673
                     /locus_tag="VBD85_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133823.1"
                     /translation="MIRRAKEKDLPDIAELLKQILMLHHEVRPDIFHTRGSKFSKEQL
                     KEMLIDESKPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            13673..14107
                     /gene="msrB"
                     /locus_tag="VBD85_001268"
     CDS             13673..14107
                     /gene="msrB"
                     /locus_tag="VBD85_001268"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO7133824.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFD"
     gene            14259..>17036
                     /locus_tag="VBD85_001269"
     CDS             14259..>17036
                     /locus_tag="VBD85_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO7133825.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALEKMLPSYSRVLRKGSEEKILSEQLVPGDIVLIEEGDRISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENDQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKALSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSR"
BASE COUNT         5794 a         2642 c         3330 g         5270 t
ORIGIN      
        1 aataggcctt gacatcggta ctaattccgt cggatggagc attattacag atgattataa
       61 agtacctgct aagaagatga gagttttagg gaacactgat aaagaatata ttaagaagaa
      121 tctcataggt gctctgcttt ttgatggcgg gaatactgct gcagatagac gcttgaagcg
      181 aactgctcgt cgtcgttata cacgtcgtag aaatcgtatt ctatatttac aagaaatttt
      241 tgcagaggaa atgagtaaag ttgatgatag tttctttcat cgattagagg attcttttct
      301 agttgaggaa gataagagag ggagcaagta tcctatcttt gcaacattgc aggaagagaa
      361 agattatcat gaaaaatttt cgacaatcta tcatttgaga aaagaattag ctgacaagaa
      421 agaaaaagca gaccttcgtc ttatttatat tgctctagct catatcatta aatttagagg
      481 gcatttccta attgaggatg atagctttga tgtcaggaat acagacattt caaaacaata
      541 tcaagatttt ttagaaatct ttaatacaac ttttgaaaat aatgatttgt tatctcaaaa
      601 cgttgacgta gaggcaatac taacagataa gattagcaag tctgcgaaga aagatcgtat
      661 tttagcgcag tatcctaacc aaaaatctac tggcattttt gcagaatttt tgaaattgat
      721 tgtcggaaat caagctgact tcaagaaata tttcaatttg gaggataaaa cgccgcttca
      781 attcgctaag gatagctacg atgaagattt agaaaatctt cttggacaga ttggtgatga
      841 atttgcagac ttattctcag cagcgaaaaa gttatatgat agtgtccttt tgtctggcat
      901 tcttacagta atcgacctca gtaccaaggc gccactttca gattctatga ttcagcgtta
      961 tgatgaacat agagaggact tgaaacagtt aaaacaattc gtaaaagctt cattgccgga
     1021 aaaatatcaa gaaatatttg ctgattcatc aaaagatggc tacgctggtt atattgaagg
     1081 taaaactaat caagaagctt tttataaata cctgtcaaaa ttgttgacca agcaagaaga
     1141 tagcgagaat tttcttgaaa aaatcaagaa tgaagatttc ttgagaaaac aaaggacctt
     1201 tgataatggc tcaattccac accaagtcca tttgacagag ctgaaagcta ttatccgccg
     1261 tcaatcagaa tactatccct tcttgaaaga gaatcaagat aggattgaaa aaatccttac
     1321 ctttagaatt ccttattata tcgggccact agcacgtgag aagagtgatt ttgcatggat
     1381 gactcgcaaa acagatgaca gtattcgacc ttggaatttt gaagacttgg ttgataaaga
     1441 aaaatctgcg gaagctttta tccatcgtat gaccaacaat gatttttatc ttcctgaaga
     1501 aaaagtttta ccaaagcata gtcttattta tgaaaaattt acggtctata atgagttgac
     1561 taaggttaga tataaaaatg agcaaggtga gacttatttt tttgatagca atattaaaca
     1621 agaaatcttt gatggagtat tcaaggaaca tcgtaaggta tccaagaaga agttgctaga
     1681 ttttctggct aaagaatatg aggagtttag gatagtagat gttattggtc tagataaaga
     1741 aaataaagct ttcaacgcct cattgggaac ttaccacgat ctcgaaaaaa tactagacaa
     1801 agattttcta gataatccag ataatgagtc tattctggaa gatatcgtcc aaactctaac
     1861 attatttgaa gacagagaaa tgattaagaa gcgtcttgaa aactataaag atctttttac
     1921 agagtcacaa ctaaaaaaac tctatcgtcg tcactatact ggctggggac gattgtctgc
     1981 taagttaatc aatggtattc gagataaaga gagtcaaaaa acaatcttgg actatcttat
     2041 tgatgatggt agatctaatc gcaactttat gcagttgata aatgatgatg gtctatcttt
     2101 caaatcaatt atcagtaagg cacaggctgg tagtcattca gataatctaa aagaagttgt
     2161 aggtgagctt gcaggtagcc ctgctattaa aaagggaatt ctacaaagtt tgaaaattgt
     2221 tgatgagctt gttaaagtca tgggatacga acctgaacaa attgtggttg agatggcgcg
     2281 tgagaatcaa acaacaaatc aaggtcgtcg taactctcga caacgctata aacttcttga
     2341 tgatggcgtt aagaatctag ctagtgactt gaatggcaat attttgaaag aatatcctac
     2401 ggataatcaa gcgttgcaaa atgaaagact tttcctttac tacttacaaa acggaagaga
     2461 tatgtataca ggggaagctc tagatattga caatttaagt caatatgata ttgaccacat
     2521 tattcctcaa gctttcataa aagatgattc tattgataat cgtgttttgg tatcatctgc
     2581 taaaaatcgt ggaaagtcag atgatgttcc tagccttgaa attgtaaaag attgtaaagt
     2641 tttctggaaa aaattacttg atgctaagtt aatgagtcag cgtaagtatg ataatttgac
     2701 taaggcagag cgcggaggcc taacttccga tgataaggca agatttatcc aacgtcagtt
     2761 ggttgagaca cgacaaatta ccaagcatgt tgcccgtatc ttggatgaac gctttaataa
     2821 tgagcttgat agtaaaggta gaaggatccg caaagttaaa attgtaacct tgaagtcaaa
     2881 tttggtttca aatttccgaa aagaatttgg attctataaa attcgtgaag ttaacaatta
     2941 tcaccatgca catgatgcct atcttaatgc agtagttgct aaagctattc taaccaaata
     3001 tcctcagtta gagccagaat ttgtctacgg cgactatcca aaatataata gttacaaaac
     3061 gcgtaaatcc gctacagaaa agctattttt ctattcaaat attatgaact tctttaaaac
     3121 taaggtaact ttagcggatg gaaccgttgt tgtaaaagat gatattgaag ttaataatga
     3181 tacgggtgaa attgtttggg ataaaaagaa acactttgcg acagttagaa aagtcttgtc
     3241 ataccctcag aacaatatcg tgaagaagac agagattcag acaggtggtt tctctaagga
     3301 atcaatcttg gcgcatggta actcagataa gttgattcca agaaaaacga aggatattta
     3361 tttagatcct aagaaatatg gaggttttga tagtccgata gtagcttact ctgttttagt
     3421 tgtagctgat atcaaaaagg gtaaagcaca aaaactaaaa acagttacgg aacttttagg
     3481 aattaccatc atggagaggt ccagatttga gaaaaatcca tcagctttcc ttgaatcaaa
     3541 aggctattta aatattaggg ctgataaact aattattttg cccaagtata gtctgttcga
     3601 attagaaaat gggcgtcgtc gattacttgc tagtgctggt gaattacaaa aaggtaatga
     3661 gctagcctta ccaacacaat ttatgaagtt cttatacctt gcaagtcgtt ataatgagtc
     3721 aaaaggtaaa ccagaggaga ttgagaagaa acaagaattt gtaaatcaac atgtctctta
     3781 ttttgatgac atccttcaat taattaatga tttttcaaaa cgagttattc tagcagatgc
     3841 taatttagag aaaatcaata agctttacca agataataag gaaaatatat cagtagatga
     3901 acttgctaat aatattatca atctatttac ttttaccagt ctaggagctc cagcagcttt
     3961 taaatttttt gataaaatag ttgatagaaa acgctataca tcaactaaag aagtacttaa
     4021 ttctacccta attcatcaat ctattactgg actttatgaa acacgtattg atttgggtaa
     4081 gttaggagaa gattgatatg gcaggttggc gaaccgttgt tgtaaataca cattctaagc
     4141 tctcttataa aaataatcat ctgattttta aagattctta tcagacggaa atgattcatc
     4201 tatcagagat tgacattcta atcatggaaa caacagatat cgttttgtcg accatgctga
     4261 ttaaacgttt ggttgatgaa aatattttag ttatattttg tgacgataaa cgcttgccaa
     4321 cagctatgtt aatgccgtac tatgccagac atgattcgag tttacaatta tctaggcaga
     4381 tgtcatggat tgaagatgtc aaagcagatg tttggacatc aattattgca caaaaaattt
     4441 tgaatcagtc tttttatctc ggtgagtgtt ctttctttga aaaatcccag tctattatga
     4501 atctctacca tgacttagaa ccttttgatc cttctaatcg tgaggggcat gctgctagga
     4561 tttatttcaa tacacttttt ggaaatgatt tttcaagaga gcaggataat ccaataaatg
     4621 ctggtttaga ctacggatat tcattgcttt tgagtatgtt tgcgcgtgaa gttgttaagt
     4681 gtggttgcat gacacaattt ggcttgaagc atgctaatca atttaatcag ttcaacctag
     4741 caagcgatat tatggaacca tttcgcccaa tcgttgatag gattatttat gaaaataggc
     4801 agagtgattt tgtcaaaatg aaaagagaac tcttttctat gttttcagag acatacagct
     4861 acaatggtaa agaaatgtat ctctcaaata ttgtcagcga ctataccaaa aaagttatta
     4921 agtcgctaaa tagtgatggg aatggaattc cggagtttag gatatgagtt atcggtatat
     4981 gcgaatgatt ttaatgtttg atatgcctac tgaaacagca gaagaacgga aggcgtatcg
     5041 taagtttaga aagtttctct tgagcgaagg ctttatcatg catcagtttt ctgtttatag
     5101 taaattatta ctcaataata cagctaataa tgctatgata ggtcggctta aagtgaataa
     5161 tcctaaaaag ggtaatatca cactcttaac agttacggaa aaacaatttg cgagaatggt
     5221 ttacctccat ggagaacgca acacaagtgt tgccaactct gatagtcgct tggttttcct
     5281 aggagattct tatgatcaag attaattttc caattttaga tgaaccatta gtgttaagta
     5341 atgctacgat tttaacgata gaagatgttt cagtttattc ttcattggtg aaacattttt
     5401 atcaatatga cgtagatgaa catttgaaat tatttgatga taagcagaaa agtctgaagg
     5461 caacagagtt aatgctggtt acagatatct taggatacga tgtcaactca gcacctattc
     5521 taaagttgat acatggtgac ttagaaaatc aattcaacga aaagccagaa gtgaaatcaa
     5581 tggtagaaaa attagcagct actattacag aacttatcgc atttgagtgt ctagagaatg
     5641 agcttgattt agaatacgat gaaattacga ttttagaact cattaaggca ctgggagtca
     5701 aaattgagac acagagcgac actatctttg aaaaatgttt tgaaattata caagtttacc
     5761 attatttaac gaaaaagaat ctcttggttt ttgttaatag cggagcttat cttaccaaag
     5821 atgaagttat aaaattatgt gaatacatca atttaatgca aaagtcagta ctctttctag
     5881 aacctagaag actctatgat ttaccgcaat atgttattga taaggattat ttcttgatag
     5941 gcgaaaatat ggtataatat tagtaaaagc acagtaataa caaggaatca tcgaaactga
     6001 agtcctgctg agacgaatgg cgcgattacg aaagctcaaa agaaaatttt ctacgaggtt
     6061 ttagagctgt gctgtttcga atggttccaa aacgtttttc accatttcta aaataatagc
     6121 caggttttag agctgtgctg tttcgaatgg ttccaaaaca attggaacta gcggagttgt
     6181 aaaacatttg ttttagagct gtgctgtttc gaatggttcc aaaacttata tccgtagtaa
     6241 aaataatcaa acaatgtttt agagctgtgc tgtttcgaat ggttccaaaa ccaaagtgac
     6301 aactcttaca acagtccatt gttttagagc tgtgctgttt cgaatggttc caaaacacaa
     6361 caattttcag agagtgataa gccatcgttt tagagctgtg ctgtttcgaa tggttccaaa
     6421 accggaacaa tcaatcgaat tgaacgtatt gtgttttaga gctgtgctgt ttcgaatggt
     6481 tccaaaacat ttttctttag gctacgacgg ataagcttgt tttagagctg tgctgtttcg
     6541 aatggttcca aaacatcttc ttttgaccta acaaaaggat atgtgtttta gagctgtgct
     6601 gttattatgc taggacatca ttgtggtgtt ctagtttttt gttatactga aataaatttt
     6661 cagagaatgt gggggaaggc ggtaattaga ttaattcaag acgtaattca gaacttagtt
     6721 ggccaagcta acgaaatcac cccaatttat cagtttgatt gggaaactta tatattggcg
     6781 actaaaaaat atgaacgtca tttagaggtg tgtctattag tagaaaattc gaattgtttt
     6841 tcggattcaa agagaatgtg tcaataaaag agatatgaaa ggctataatt ccaaccttat
     6901 ggttaaaggg ctaggttgtt ctacgcttta cttaattatt agtttgacag cgttggttct
     6961 tttagtgatt gctggtgttt tctttgtcat taatacttgc aagcttacaa ggaaagcagt
     7021 ggagaaccta tccataatca actaacagct atgatggatc tcacctatta atatagttaa
     7081 ggagtgtcca atactccttt tttgaaatac agttggaagt tacagaaaac atattttcat
     7141 gttataatag tggagaaaga aatgaggtgc taatattatg gaacaaacct tttttatgat
     7201 aaaacctgat ggtgtaaaac gtggttttat tggagaagtt attagtcgta ttgagcgtcg
     7261 aggcttctct attgatcgct tagaggttag atatgcagat gcagatattt tgaaaagaca
     7321 ttatgcagaa ctgacggata gaccattctt tccaacatta gtagattata tgactagtgg
     7381 tccagtaatc attggagtta tttcgggaga agaggtcatt tcaacttggc gcaccatgat
     7441 ggggagtact aaccccaaag atgcccttcc aggaactatt cgaggagatt ttgcacaagc
     7501 tccctctcca aaccaagcga catgtaatat tgttcatggg tcagactcac ctgaatctgc
     7561 aactcgcgag atagccattt ggttcaataa ctaatagact taatgaagat tgatgttcaa
     7621 gtgttaattc tcttttacat cggtcttctt tttgatataa tattctagag tctataggac
     7681 tgttttaata ctttagaagt taaacatatc aaattgtata ggaaatagaa tgaatattga
     7741 agatttgaag aaacgtcaag aaaagattag aaacttttcg attatcgctc atattgacca
     7801 tggaaaatca accttagcag accgtatttt agagaaaaca gaaacggttt ctagtcgtga
     7861 aatgcaggcg caactgttgg actctatgga tctagaacgt gagcgtggaa ttactataaa
     7921 gttaaatgct attgagctta attatactgc taaagatggt gaaacttaca tttttcactt
     7981 gattgacacg ccagggcatg ttgacttcac atatgaagta tcacgttcat tggcagcttg
     8041 tgagggtgct atactggttg ttgatgcagc ccaaggaatt gaagctcaaa ctctggcaaa
     8101 tgtttatttg gctctagaca atgatttaga gatactacca gttattaata aaattgattt
     8161 accagcagca gatccagaac gtgtacgtgc agaagtagag gatgttattg gtctagatgc
     8221 ttcagaagct gtgcttgcct cagctaaggc agggattggt attgaagaaa ttttagagca
     8281 gatcgttgaa aaagttcctg caccgactgg ggaagtagat gctcctcttc aagctctaat
     8341 ttttgattct gtttatgatg cttatcgtgg agtaattctt caagtaagga ttgtaaatgg
     8401 tatggttaag ccaggagata agattcaaat gatgtctaat ggtaaaacct tcgacgttac
     8461 tgaggttggt attttcactc ccaaagcagt tggacgtgat tttttagcaa ctggagatgt
     8521 agggtatatt gctgcctcaa ttaaaacggt tgctgataca cgtgttggtg ataccattac
     8581 ccttgctaat aacccagcaa tagaaccctt gcatggctat aagcaaatga acccaatggt
     8641 ttttgccggt ctttatccaa ttgaatcaaa taagtataac gatctgcgtg aagcccttga
     8701 aaaattacaa ttgaatgatg ctagtctaca gtttgaaccg gaaacatcac aagcattagg
     8761 gtttggtttc cgttgtggtt tcttaggact tttacatatg gacgttatcc aagagcgctt
     8821 ggaacgagag ttcaatattg atcttattat gactgcacca tcagtagttt atcatgttaa
     8881 tacaactgac ggtgaaatgc ttgaagtttc gaatccatct gaatttcctg atcctactcg
     8941 tgttgacagc attgaagaac catatgttaa ggcgcaaatt atggttcctc aagaatttgt
     9001 cggtgctgta atggagttag cacaacgtaa acgtggtgac tttgtaacaa tggattatat
     9061 tgatgataat cgtgttaatg ttatttacca aattccttta gctgaaattg tgtttgactt
     9121 ctttgataag cttaaatcat caacacgtgg ctatgctagt tttgattatg aaatttccga
     9181 atatcgtcgc tcacaattag ttaaaatgga tattctttta aatggtgata aagtggatgc
     9241 tttaagcttt attgttcaca aagaatttgc ctatgaacgt ggtaaattga tcgttgataa
     9301 gttgaaaaaa atcattcctc gtcagcagtt tgaagttcct atccaagcag ctattggaca
     9361 aaaaattgtg gcccgttctg atataaaagc actacgtaaa aatgtcttag caaaatgtta
     9421 tggtggtgac gtttcacgta aacgtaagtt gcttgaaaaa caaaaagctg gtaaaaagcg
     9481 catgaaagca attggttccg tcgaagttcc tcaggaagct ttcttaagtg ttttatcaat
     9541 ggacgatgat gacaaaaaat aattcagaaa gatgatatca tttatctatt ttaaagtaac
     9601 ttagcttcgt gcttaagtta ctttttcttt gtctgcaatt atcaaataat atggctttat
     9661 aatatatttg tttaaatata aatgagctat aaatgagttt ttgttagtaa ctaaataata
     9721 aaaagatggc aaatagtatt gcaaaaagaa aaacatctga tataattaac tagttaacta
     9781 aaacgaaaaa aacatttatg gaggactcat gaaaggtcaa aaattattgc tctagctggt
     9841 cttgtcctgt catgccattt tgctcttacg gcatgtcata ctcaagaaca caaaaattct
     9901 catcatatta aaacaaagca ggttgctaag aaaaaagcta ataaaaagaa agtctctgtt
     9961 aaggaaagtc ataaaaaacg caagggtgtt gcaggggttg actttccaac tgatgatggc
    10021 tttttactga ctaaagattc gaagatttta tctcatacag attctggaat cgttgtagca
    10081 catggaaatc attctcattt tattttttac tctgatttaa aaggttcaaa atttagctat
    10141 ttaatcccta atgctaatac aaaaactaat aaaaatcaag cggtaagaaa ctttaaagca
    10201 ggggcggtag ctgttaatac attaaatgat ggctatgttt ttaatcccgc agatattgtt
    10261 tcagaagatg ctaatggcta cgtggtcaga catggtgatc atttccacta tattccaaaa
    10321 gcaagtttat ctcagcaaaa gcaagtacaa gcaagtagag ctgtttcacg tttaggaaat
    10381 caaagtaata gccattacag ggttaatagt tctaaaattg cagggcttca ctacccaaca
    10441 agtaatggtt tcttatttaa tggacgagga ataaaaggca ctaccccaac aggtatttta
    10501 gtagaacatc ataatcatct gcattttatt agttttgctg atttaagaaa aggggggtgg
    10561 ggatcaattg ctgaccgtta ccaaccacaa aagaaagctg attctaaaaa acagtcacca
    10621 tcaagtaaaa agccaagaac tgaaaatact ttacctaagg acatcaaaga taagctagct
    10681 taccttgctc gtgaattaca tttagatatt tcacgtatca gagtccttaa aacactaaat
    10741 ggtgaaattg gatttgaata cccacatgat gatcatacac atgttattat ggccaaagat
    10801 attgatctaa gtaagccaat tccaaatcca catcatgatg atgaagatca tcacaaaggt
    10861 catcaccatg atgagtccga ccataagcat gaagaacacg agcacactaa gtcaaataaa
    10921 ttatctgatg aagatcaaaa gaaattaatt tatttagcag agaagcttgg tttaaatcct
    10981 aatcaaattg aagtattaac gtcagaggat ggaagtatca tctttaagta tccacatgat
    11041 gaccactcac acaccatagc aagtaaggat attgaaattg ggaagcctat cccagatgga
    11101 caccacgatc actctcatgc aaaagataaa gttggtatgg caactctaaa acaaattggt
    11161 tttgatgatg agattattca ggatattctg catgcagatg ctccaacacc attcccatca
    11221 aatgaaacta atcctgaaaa aatgcgtcag tggcttgcaa ctgttactaa gattaacatt
    11281 ggacaaagaa ccaatccatt ccagcgtttt ggtctatcat tgatgcctaa tattgaagtt
    11341 ctaggaattg ggtttactcc gattaatgat atgacgccag ttttgcaatt caaaaaactt
    11401 aaacagcttt ggatgacaaa tactggtatt acagattatt ctttccttga taagatgcca
    11461 ctattagaag gattggatat ttcacaaaac ggtattaagg atttatcttt ccttacaaaa
    11521 tataaacaat taagtcttat tgctgcagct aataatggca ttacttcgct aaaaccattg
    11581 gctgaattac caaaccttca attcttagtt ttgagtcata acaatatctc tgacttgaca
    11641 ccgttatcaa acttgacaaa actccaagaa ttgcaccttg atcataataa tgtgaagaat
    11701 ttaagtgcac tttctggtaa aaaagatttg aaagttctag atttatcaaa caataaatcc
    11761 gcagacttat cgactctaaa aacaactagt cttgagacgc ttcttttaaa tgagacgaat
    11821 acaagcaatc taagtttctt aaagcaaaat cctaaggtat ctaatttaac aattaataat
    11881 gcgaaattag catcattaga tggtattgaa gagagcgatg aaattgttaa agtagaagct
    11941 gaagggaacc aaattaagtc actagtattg aaaaataaac aaggttcgtt aaaattcttg
    12001 aatgtgacta ataatcagtt aacatcactt gaaggtgtta ataattatac ttcacttgag
    12061 accttaagtg tttctaaaaa taaacttgaa tctttagata ttaaaacacc taacaaaaca
    12121 gttacaaatc ttgattttag ccataacaat gttccaacat cacaattgaa attgaatgaa
    12181 aaaaatattc ctgaagcagt agctaagaat tttccagcag ttgtagaagg ttcaatggtt
    12241 ggaaatggta gtcttgctga aaaagcagct atggcttcta aagaggacaa acaagtttca
    12301 gataatacta atcaccaaaa aaacactgaa aaatctgctc aagcgaatgc tgatagtaaa
    12361 aaagaaaacc ctaaaacaca tgatgaacat catgaccatg aagaaacaga tcatgcacat
    12421 gtaggtcatc atcaccatta atcttttaca gagctaatat ataaaatcct tcaagaattt
    12481 cttggaggat tttgcattta tatgctaaat catttatttt tttagaaaat tagctataat
    12541 atgactatgg ttaaaagaga agatgtgatt ttaaatgcaa ctgaaaatat ggttcaccat
    12601 aaattaaaaa atgatccaag tggccatgac tggtttcata ttgttagagt aagaaatctt
    12661 gcggtagaat tagcacacaa agaaggggct aataccttta tttgtcaaat ggctgcactt
    12721 ttgcatgata ttattgatga caaaatctgc caagatagta agcaagcgtc gtatgaattg
    12781 actcaatggt tatatagtca agatcttgcc atagcagaag tagaacatat ccttgatatt
    12841 cttgaaaata tttcattcaa agcaggaact ggattgacta tgaaaacatt ggaaggtcaa
    12901 attgttcaag atgcagatcg cctagatgct atgggggcta ttggtattgc aagaacgatg
    12961 gcttattcag ggagtaaagg acgtttaatt catgacccta acttaaaacc acgagaaaac
    13021 ctcactttag aagagtatcg taatggtcag gatacagcta tcatgcattt ttacgaaaaa
    13081 ttattaaagt taaaagacct tatgaataca aaacaaggga aaatgcttgc tcaaaaacgt
    13141 catgattttt tagaactata tttagccgaa ttttatgctg aatggaatgg taaacgttag
    13201 gagtagatat gattcgtaga gcgaaagaaa aagatttgcc tgatattgca gagctattga
    13261 aacaaatttt aatgttacat catgaggttc gcccagatat ttttcataca agaggaagta
    13321 agttttctaa agaacaattg aaagaaatgc tgatagacga atcaaagcct atctttgttt
    13381 atgaatcaga cgagggtaaa gttgttgccc acttgtttct tcaattacaa gaaaaacgtg
    13441 acttacctcg aaagtcattt aaaactctct atatagatga tttgtgcatt gatgaggaag
    13501 taagaggtca gcaaattgga caaaagttga tggattttgc aagacaatat gctaagaaac
    13561 atgggtgtta taatataact ttaaatgttt ggaatgataa tcagagagcg gtctcttttt
    13621 atgaaaaatt aggttttaaa cctcaacaaa ctcaaatgga ggagatacta taatgaaaga
    13681 aacacaagaa gaattacgtc agcgtattgg acatacagct tatcaagtaa cccaaaattc
    13741 agctacggaa catgctttta ctgggaagta tgatgatttt tttgaagagg ggatatatgt
    13801 agatatcgtc agtggagagg ttttattctc ttctttagat aaatttcaat ctggttgtgg
    13861 ttggccagca ttttcaaaac caattgagaa tcgaatggtg acaaatcatc aagatcactc
    13921 tcatggaatg catcgtattg aggttaggag tcgtcaagct gactctcatt tagggcatgt
    13981 atttaatgat ggaccggttg atgcaggtgg tttacgctat tgtatcaatt cagctgcctt
    14041 agattttatt ccttatgacc agatggcaaa gagaggctat ggggactacc tatcactttt
    14101 tgattaataa gagacgactt aagggtatat cttaagtcgt tttttatgta tggtaaaaag
    14161 aattggtggc aggtgtattc ttcatcagtt tattttctga aaaaagagta taatataggt
    14221 atcaaaaatg aaaacgtttt agagaaggag actagaatat ggcttctaca aataatgttt
    14281 cagatacaaa cacacgtttt gctaaagagg agttagaaga ggttttcgaa gaattaggga
    14341 caactcaagg tggtttatca gatgaagagg ttgctgtacg tcagaaaaaa tatggtttaa
    14401 acctgttatc tgaagtaaag caagaatcga ttattttatt gtttctgaaa aattttacta
    14461 gtcttatggc aatactatta tgggttggag gatttgtggc tattgtttct aatagcttag
    14521 agctaggtct tgccatttgg atggttaatg tgattaatgg tatttttagt ttcattcagg
    14581 aataccgtgc aagccaagca acacaagctc tcgagaaaat gttaccatct tattcacgtg
    14641 ttcttagaaa aggctcagaa gaaaagatat tatcagagca actagtccca ggcgatattg
    14701 ttcttattga agaaggagac cgtatttcag cagatggtcg cttgattaaa acaacagatt
    14761 tgcaagtaaa ccaatcagcc ttgacagggg aatcaaatcc tatttataaa gatagcaatg
    14821 ttgaaaacga ccagtctaaa acattgattg aatgtgataa tatggttttc gcaggaacga
    14881 cggtctccag tggtagcgca acaatggttg ttacggcaat tggaatgcaa actcaatttg
    14941 gtcaaatcgc tgatttaaca caagggatga aatcagaaaa aagccctctt caaagagaac
    15001 ttgatcggtt gaccaaacag atttctatca tttcaattac ggtaggaatt atctttttct
    15061 tagcagcgac tttttttgtt aaggagccag tgtcaaaatc atttatcttt gccttgggga
    15121 tgatcgttgc atttatccca gaaggtttac tacctaccgt tacactttct ctagctatgg
    15181 cagtccaacg aatggctaaa gaacacgcac ttgtcaaaaa actttcttca gttgagactc
    15241 ttggagcaac ctcagttatc tgttctgata agactggtac attaacgcaa aatgaaatga
    15301 ccgtcaatca tttgtggcaa aatggaaaga gttaccaagt gacaggactg ggttatgctc
    15361 cagaaggaca gattcttttt gaaggtgaca atatttgctt tggtaatagt gatagaggag
    15421 acttagaaaa acttattcgt tttgctcatc tctgtagcaa cgctcaagta ttaccaccaa
    15481 atgatgatcg ttcaacttat actgtacttg gagatccgac agaagcttgt ttgaatgttt
    15541 tattggaaaa atcagggata aacatacaag aaaatcgtaa gtttgcacca cgtttgaaag
    15601 aattaccttt tgattcggtt agaaaacgca tgacaactat tcatagctta ggaggcgatg
    15661 aaaaggataa aaaaatttca attacaaaag gtgcgccgaa agaaatactt gatttatcag
    15721 attatgtctt atctgatggc aaagttatcc ccttaaacaa ggaagaacgt aataaaatac
    15781 agttggctaa cgatacattt gctaaagatg gacttcgcgt cttagctgtt tcatactgcg
    15841 atattgaagg cttttcaaaa gaacaatgga cgcaagaaaa tttggaacaa cacatggtgt
    15901 ttatagggtt gatagcaatg agtgatccac ctcgtgaagg cgttcgcgaa gctattgata
    15961 aatgtcatgc agcaagcatt cgtattatta tggtgactgg agattacgga ttaacggcac
    16021 taagtatcgc taaaaatatt ggtattattc gtaacgatga tgctaaagta atttcaggtt
    16081 tagagttatc agaaatgacg gatagccaat tgaaaaaaga attgtcaggt gaagtcgttt
    16141 ttgctcgtgt agcaccagaa caaaaatatc gagtagtaac gatattacag gagatgggtg
    16201 aagtagtagc agttacagga gacggggtca atgatgcgcc tgcgctaaaa aaatccgata
    16261 ttggtgtagc catgggtgtt accggtactg atgttgctaa agaatcagct gatatgattc
    16321 tgacagatga tcattttgct tctattgtac atgctgtgga agaaggacgg gctgtttatc
    16381 aaaacattaa gaaattcttg acgtatattt ttaatagtaa tactcctgag gctgttccgt
    16441 cggcattctt ccttttttct aaaggtttta ttcctttacc attgactgta atgcaaatac
    16501 ttgctgttga tttgggaacc gatatgcttc ccgctttagg tctgggcgtg gaaccaccag
    16561 aaacagacgt tatgaatcgt cctccaagac ggctaacaga tcgcttattg gacaaagggc
    16621 tacttataaa atctttcctt tggtacggca ctattgaatc cgtattagct atgggtggtt
    16681 ttttctgggc tcattactta cgttatggta attttacttt tttcgtcgca aacggcatac
    16741 cgtacagaga agcgacaact atgacacttg gagctattat ctttagccag ataggtatgg
    16801 ttatgaatag cagaacgagc tatcaatcta ttaaagcact atctattttt ggtaataaat
    16861 taattaattt tggtataata atggagattt tagcattttt agtcttagta tacgtgccac
    16921 tattccataa cttattcaac acagcaagcc taggtttatc acattggctc tatttaataa
    16981 gctgcccatt tatcatgata ggtttagatg aagttcggaa attattcagc agtaga
//
LOCUS       DATRSD010000048        16952 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRSD010000048 DATRSD010000000
VERSION     DATRSD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16952
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.048"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            316..1485
                     /locus_tag="VBD85_001270"
     CDS             316..1485
                     /locus_tag="VBD85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7133826.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1482..1628
                     /gene="rpmG"
                     /locus_tag="VBD85_001271"
     CDS             1482..1628
                     /gene="rpmG"
                     /locus_tag="VBD85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7133827.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1675..1911
                     /gene="secG"
                     /locus_tag="VBD85_001272"
     CDS             1675..1911
                     /gene="secG"
                     /locus_tag="VBD85_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO7133828.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2024..4429
                     /gene="rnr"
                     /locus_tag="VBD85_001273"
     CDS             2024..4429
                     /gene="rnr"
                     /locus_tag="VBD85_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO7133829.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYTAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4432..4899
                     /gene="smpB"
                     /locus_tag="VBD85_001274"
     CDS             4432..4899
                     /gene="smpB"
                     /locus_tag="VBD85_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO7133830.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4983..6371)
                     /locus_tag="VBD85_001275"
     CDS             complement(4983..6371)
                     /locus_tag="VBD85_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7133831.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6500..7426)
                     /locus_tag="VBD85_001276"
     CDS             complement(6500..7426)
                     /locus_tag="VBD85_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7133832.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7555..7947)
                     /gene="gloA"
                     /locus_tag="VBD85_001277"
     CDS             complement(7555..7947)
                     /gene="gloA"
                     /locus_tag="VBD85_001277"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO7133833.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     ALEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8035..8709)
                     /locus_tag="VBD85_001278"
     CDS             complement(8035..8709)
                     /locus_tag="VBD85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7133834.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8976..9818
                     /locus_tag="VBD85_001279"
     CDS             8976..9818
                     /locus_tag="VBD85_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7133835.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGAIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            10009..10731
                     /locus_tag="VBD85_001280"
     CDS             10009..10731
                     /locus_tag="VBD85_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7133836.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNGNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLLTNNGELAHKMLSPKKHVDKCYEVKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLRMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKYMQ"
     gene            10816..12858
                     /locus_tag="VBD85_001281"
     CDS             10816..12858
                     /locus_tag="VBD85_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7133837.1"
                     /translation="MKRKYFILNTVTVLTLAAAMNTSSIYANSTETSASVVPTTNTIV
                     QTNDSNPTAKFVSESGQSVIGQVKPDNSAALTTVDTPHHISAPDALKTTQSSPVVEST
                     STKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAI
                     EEARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLG
                     FIILGQTNFPEYGWRNITDSKLYGLTHNPWDLAHNAGGSSGGSAAAIASGMTPIASGS
                     DAGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKS
                     DQTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDG
                     RALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSI
                     MEAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEEDKRAIYNMENLSQE
                     ERIALFNRQWEPMLRRTPFTQIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVL
                     IKFATFFEKHHGFNVKWQRIIDKEVKPSTGLIQPTNSLFKAHSSLVNLEENSQVTQVS
                     ISKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            12968..13546
                     /locus_tag="VBD85_001282"
     CDS             12968..13546
                     /locus_tag="VBD85_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7133838.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGRTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDGKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSFYNKKMKLY"
     gene            complement(13580..14821)
                     /locus_tag="VBD85_001283"
     CDS             complement(13580..14821)
                     /locus_tag="VBD85_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO7133839.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEAAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSETTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDDPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETVQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGTAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            15018..15146
                     /locus_tag="VBD85_001284"
     CDS             15018..15146
                     /locus_tag="VBD85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO7133840.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            15207..16520
                     /gene="obgE"
                     /locus_tag="VBD85_001285"
     CDS             15207..16520
                     /gene="obgE"
                     /locus_tag="VBD85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO7133841.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDATT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYVSINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     ELLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            16546..16704
                     /locus_tag="VBD85_001286"
     CDS             16546..16704
                     /locus_tag="VBD85_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133842.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         5738 a         2714 c         3321 g         5179 t
ORIGIN      
        1 actaaatcta taacgatgcc aatattatta gtaagcctag gaattatagt tgttatttta
       61 attattggct atttagcagt tgtagtattt gctccaaaaa ctttccgttt aaaaagttaa
      121 ttttaattgt ggtataataa cagaggcatg tgataacata ggtttagtta cataaaggaa
      181 attataatgg aaataacatc tcaaatagat gttaaatctc aataaaactg ctaagatagt
      241 tagtggtaga aaagtttaga aagaagatga tgttttatct cagttgaaac ggttaactgg
      301 aagcaaaatt taaaagtggc ttggctcggt aattttttta ctggggcaag cttttcactt
      361 gttatgcctt ttatggtatt atatgtagaa caattaggag cacctagcaa caaagtagag
      421 tggtatgcag gcctatcagt atccttatca gccttgtctt cagctttagt tgccccatta
      481 tgggggcgct tagctgataa atatggtaga aaaccgatga tggtaagagc agggttgatg
      541 atgactttta ctatgggagg tcttgctttc attcatagtg ttacaggact tctgatttta
      601 cgtatattaa acggcatttt tgcaggctat gtacctaact ctacagcttt gatagcaagt
      661 caagctcctc aagaggaatc aggatatgcc ttaggaacct tagcgacagg agtgactgga
      721 ggtatgttaa ttggtccact tttaggggga cttttagcag agtggtttgg tattagggaa
      781 gtttttttat tagtgggaac catattgtta atatctactt taatgaccat ttttatggta
      841 aaggaagatt tcaaacccat ttctaacgaa gaaaccatgc caactacaga agtgttcaaa
      901 agcgttaaaa gcttacaaat tttaataggg ctgtttgtaa caagcatgat tattcaaatt
      961 tctgcacaat caattgcacc tattttaaca ctttacattc gtcatttagg acagactgaa
     1021 aacttgatgt ttgtttccgg attaattgtt tctggtatgg gattctctag tattctgagt
     1081 agtccaaagc taggaagaat cggggatcgc ataggcaatc atcgtttgct attattagct
     1141 ctactgtata gtttcctcat gtatgtctta tgttcattgg cacagacttc attacagcta
     1201 ggggttatta gattcctgta tggttttgga acaggtgcct tgatgccgag tattaattca
     1261 atcttgacta aaattgctcc aagacaaggt ttatcaagga ttttcagtta taaccaaatg
     1321 tttagcaatt taggacaagt attaggtcct tttgtcggtt cagcagtttc tattcattta
     1381 ggattccgat gggttttctt tgtgactagc ttcatcgttt tagctaattt tgtttggtgt
     1441 tttattaatt ttagaaaata tattagagtt aaggaaattg tgtgagagtt aaaattaatt
     1501 taaagtgtag ctcttgtggt agtaagaatt atttaaccag taagaataaa agtaatcatc
     1561 ccgataaaat cgaggttcca aagtattgcc caaaggagag aaaagtaaca ctacatattg
     1621 aatcttaata agttttatgt tagaatgtac tttgaccata gtggaggaat taacatgtat
     1681 aacctattac tcaccattct acttgtttta tcagtattat tgattatctc aattttcatg
     1741 caaccacaaa agaatccaag tagtaatgtt tttgatagta gcggttctga agcccttttt
     1801 gaacgtagta aagctcgagg atttgaagct tttatgcaac gttttacagg tgttttagtc
     1861 tttttctggc tactaatcgg cttagtatta tctattttat caagtcatta atgatagcta
     1921 aatacagata agtgaggatg ggggatagcc caacctcact ttttgttagt agaatggttg
     1981 tagagtaata ataaagtgac taaagttact agaaaaggaa ataatgaaag aaaaaattat
     2041 aaaatatttg gaagaacacg gtaaaaccag cgttaatgat ttagctgctg ccctagatat
     2101 ggcaggcgca aaagcatttc caaaattaat caaaacaatc tcaaatttgg agagccacag
     2161 gcaattaaga tttgacgata atggaagtct ttctcttcaa aagaaagaag ctaagaaaaa
     2221 ggaaatcaca gtaaggggtc tttttagagc aaataaagca ggatttggtt ttcttagtat
     2281 tgaccaagac gaagatgata tgtttatcgg aaaaaacgat attgcttatg ctattgatgg
     2341 tgacacggta gaagctgttg taaagaaacc agcggaccgt cttaacggga ctgcagcaga
     2401 agcgcgtgtt gttaatattg ttgaacgtag tttaaagacg cttgttggta agtttgtctt
     2461 agacgatgag cgccctaaat acgcaggata tatcaaatcg aaaaatcaaa aaatcaatca
     2521 aaagatttat atacgcaaag aaccggtcgt tttagacgga acagaaatta tcaaagtaga
     2581 tattgataaa tatccgacac gaggacatga ttacttcgta gcaagtgttc gtgatatcgt
     2641 tggacatcaa ggagatgttg gtattgatgt tttagaagtc ttggaaagta tggatatcgt
     2701 ctcagaattt cctgaggacg ttattgcaga agccaatgct atcccagatg ctccaacgga
     2761 gaaagattta attgggcgtg ttgatttacg tcaagaggtc actttcacta ttgatggagc
     2821 ggacgccaaa gatttagacg atgctgtgca tattaaatta ctggataatg gtcattttga
     2881 acttggagtt catatcgcag atgtatcata ctacgttact gaaggctctg ccttaaatcg
     2941 tgaagcactt agtcgtggta cgtccgtata tgtcacagat cgcgttgtac caatgttacc
     3001 agagcgtcta tctaacggta tatgttcatt aaatccaaat cttgatcgtt taacgcagtc
     3061 ttgtattatg gaaattgacc aaaatgggag agttgttaat catcaaatta cacagtcagt
     3121 gattaatacc acttatcgta tgacttatac cgctgtaaat gatattattg ctggcgatga
     3181 ggaaatctgc tcagaatatg agtctattgt gtcctcagta cagcatatgg tcactttaca
     3241 ccatactttg gaagctatgc gtacgcgtcg tggagcgctg aattttgaca catctgaagc
     3301 aaaaattatg gttaatgata agggaatgcc agttgatatt gttattcgca acagaggcat
     3361 tgctgaacgc atgattgagt catttatgtt ggcggcaaat gaaacggttg ctgaacatta
     3421 tgctcgtcta aaactgccat ttatttatcg tatacacgaa gagcctaaag ctgaaaaatt
     3481 acaaaaattc atcgattatg cttctgtttt cggtgttcag attcaaggaa cagcaactaa
     3541 aatcacccaa tctgctttac aggactttat gaaaaaagta caagggcagc caggaagtga
     3601 agtgttatct atgatgttat tgcgctcgat gcaacaagct agatattctg aacacaacca
     3661 tggacattat ggacttgctg cggaatatta cactcacttt actagcccca ttcgacgtta
     3721 tccagacttg ttagttcatc gtatgattcg tgattatgat gataaagcga tggataaagc
     3781 tgatcatttt gctaacctta tcccagaaat tgcaactcaa acgtctagtc ttgagagacg
     3841 tgccattgat gctgaacgta ttgtagaggc aatgaagaaa gccgaatata tggaagaata
     3901 tgttggtgag gaatttgaag gtgtggttgc aagcgttgtt aaatttggta tgtttgtaga
     3961 attaccaaat actattgaag gattgattca cgtaacgacc ttgcctgaat attatcactt
     4021 taatgaacgt acgctgacgc ttcaaggtga gaaatctggg aaagttttcc gtgttggtca
     4081 acaaattaag gttaagttga ttcgttctga taaggaaact ggtgatatcg attttgatta
     4141 tttaccatca gattttgata ttgttgaaaa ggtaagtaaa agttcgcgtg agggtcgtcc
     4201 taatagaagt tctaaacgag aacatcagca tagaatttct gatcgtgata ataagaataa
     4261 aaatacatct aagaaaaaag catctcgaaa acccaaaagg aactcggata gcaaatcaca
     4321 tcatcataaa gacgatagaa caacaggttc aaccaagaaa aaaacgaaaa aacctttcta
     4381 taaaggcgtt gctaagaaag gtcagaaacg gaaatctaag aaaggttaag tatggttaaa
     4441 ggtcaaggaa acgttgtagc acaaaataaa aaagcgcatc acgactatac tatcgttgaa
     4501 acaatagaag caggaattgt tttgacagga acagaaatca aaagtgtccg cgcagcgcgt
     4561 attacattga aagatggata tgcacaaata aaaaatggtg aagcgtggtt gattaatgtt
     4621 catattacac catatgatca aggtaacata tggaatcaag atcctgatcg tacacgaaag
     4681 ttattactaa aaaaacgtga gattgaaaaa atatcaaatg aactgaaagg gactggtatg
     4741 actctggtac ctctaaaagt ttacttaaaa gatggttttg ctaaagttct attgggtttg
     4801 gcaaaaggaa aacatgatta tgataagcga gagtctatca aacgtcgtga gcaaaaccgt
     4861 gatattgccc gtcagttaaa aaactataat tcacgatgat tatcaaaagg ttaggcaatt
     4921 gcctaacctt ttttggacga aaaaagtgaa gttgattgac caacttcact aataatatag
     4981 acttaatgtg atttatgttt gaagtgacta tataaaccaa atccaataat ccagatggtt
     5041 gctccaatag ccccgatgta tgttgaatct tgcaagaaga gagaaacgaa tacaaagagg
     5101 aagaagagaa gtgtgattgg tgaaaggatt ttgtaggcag gcattttata accatcttcc
     5161 ataaaatcag gtgatttacg atatttccaa tgtgcaatca taataagaac atagatagaa
     5221 atgtaaacac cagaagatga tgcagtaatt aaggagaagg catctgaaat accaggtaaa
     5281 acactgataa gtgctgaagc tccaacgatt acagctgatg caataatagc tcgacttgga
     5341 acgctttgac gagataattg gtctaatttt aacgcttttg taagagcatt tggtgattca
     5401 ttagccaatt ggaataaatg acgccctgtt gagtacaaag tagaattaag agcagatgct
     5461 gcagacgtta ggacaacaaa gttaatcaaa gcggcagccc acttaatccc agctagtttg
     5521 aatactgtaa cgaaaggtga ctcattaact ggtaactgtt gccatgggaa gatggccata
     5581 attgctaata atgaacctgc gtagaataaa ataatacgca tcggaatttc ttggattgct
     5641 tttggtaaaa ctttacgtgg attagctgtt tcagaagttg ttactccgac aaattcaata
     5701 gctaaataag caaagaatac catttggaaa gccatgaaga aattcaattt acctttaggg
     5761 aaccattcaa aatgattggt gatatttgag attgaagcat ggtatccgtg accggtgtca
     5821 aagtttgtca ataccataaa gataccggtt gctattaaac caagaattgc aataactttt
     5881 atcatagcaa accaaaactc ggtttctccg aatgctttta cagcaattaa gttgatagaa
     5941 cttaggatag ctaaaaaaac aatttggata atccaggatg gccattcagg gaaccaaaat
     6001 tgaacatact ctcctacggc tgtaatctct gccataccga taaatattag ggaaatccaa
     6061 taagaaagtg ttgagaaaaa tccccaaccg ggtcctaaat atttggtaat gaaattaata
     6121 aaagtatgct gatttggatc ttgataaagc atctctccaa tagcacgcat catgaagaac
     6181 atgaaagcgc cagtgatagc atagacaaga actattgaag gacccgtcag gctaattgaa
     6241 cgacctgcac caagaaaaag cccagttccg attgtccctg caatcgcaat taactgtacg
     6301 tgacgatttt ctaggccgcg taccatacca ttctcagatt tttgagtgtg gtcattatta
     6361 ttttttgaca ttataaatac tccatctatt tttgaaaaac tcctttatta tataattttt
     6421 ttgcaaaatt cgcaataatt actatacaat atttttctat gataaaatct cctagctaag
     6481 tgagctaaga gatttcaatt catccctctt ccttgacaat atagactggg cgttttttag
     6541 tttctaggaa aatcttagag atgtatttgc ctataattcc tagcgacaat aactgaatac
     6601 cacccataaa aagtacaatg gtaactgtgc ttgcccaacc tgaaacggga tccccaaata
     6661 gtaattttcg aattataata aaaatgatag ctaaaagtga tagtactgaa ctaagggttc
     6721 caatccaggt agctatagct aatggaattt ctgagaagtt gatgaaaccg tctagagagt
     6781 attttaagag attaaagaaa ctccatgagg tcttacccgc aattcgttct cgattctcat
     6841 aactaatata agcgacatca tatccaatcc aggaaaaaat cccttttgag aagcgattca
     6901 cttcatttag ctcgagaata ctgtcaacaa cttgttttgt catgagacga aaatcacgcg
     6961 caccatcaac cattttagta tctgacactt gattgattaa tttataaaaa agcttggcaa
     7021 aaagagagcg tatcaacggc tcccctttac gatcttttcg ccttgtagcg acaatatcat
     7081 aaccttcttg aagttttagg tacatttctg gtaataaagt gggtggatct tgcaagtcaa
     7141 catccataac agtgataaaa tcaccagtag tatgttctaa accagccaat aaagcagcat
     7201 ctttaccaaa atggcgtgaa aatgaaaggt aatgaacatt gtcaaattgt ttagctgctt
     7261 tcttaagaag ttcgagtgta cgatcagtag aaccatcgtt tacgaaaatg tattcaaaat
     7321 gggtgtaacg agccatttcg gattcaactt ttttgatttc tgttaaaaaa gttgagacag
     7381 tctcttgttc attataacaa gggacaataa ctgataataa agccatttgt aattctccgt
     7441 atcattcttg cttaaatttt aacataatat gggaaaaaat agaaagatta taaggtaatc
     7501 ataaaaagga ctaggttttt gcctcagtcc ttaacttaat atgatgagat gttattagtc
     7561 agctttaaat tgactgagtc gaataacttc aattttatag ccatctggat cttgaataaa
     7621 gtagaacata ttaggtttac ctggtaatcc agaaattttt gttacagagt aacctgcttt
     7681 ctgatgggca tcataagtag tttccaaatc atctacacca acagcaatat gaccataccc
     7741 atttcctaaa tcataagctt cgtgatcata gttataggtt aactctaatt cgtaactagg
     7801 gtcgtcctct agggccatat agactaaagt aaattgattt tcaggaaagt cattacgacg
     7861 aacttcctta aaaccaagtg cttcttgata aaaagcaatt gaggcgtcca aatctttaac
     7921 gcgaatacaa gtatgtaaaa atggcatagt ctctctccta tttctttatt agacgtatta
     7981 tgtcaaaata aaattgtctt ttcaaaaata ttgattataa attaggctgt ctatttactc
     8041 aacccaagta atgacttctt ggcgttcttt cctagcacgg gaatgtttag gttcacgcaa
     8101 acgataacca aatgaaacca tacaagaaat agcttctttc ttatcgtcta ttaatccttc
     8161 tttactaagg atattattga ccttttcata gtcaaatcct tcaattgggc atgaatctac
     8221 gccgatcata gcagcagccg tcatcatatt accgagtgcg atataagttt gttttgctgt
     8281 ccaatcccaa agagaacgtt cagaatcaat tttcatatcg ttttcttgga aggatttgta
     8341 gagaggtatc ctactctcaa gggcagctgg atcacctaga ccacgtcgaa tgagactgtt
     8401 aatcatgcta tcagcgttat aataagcacc tttttcagct agtagtaaaa caaagtgact
     8461 agcagtatct agttgatatt gtgctcccca agcaacttcc ttaagtttat ctcgaaattt
     8521 tgcaattgtt tgtctatcta gtaccaaaaa tcgccagcct tctaaaccga ctgaagaagg
     8581 actcaaccaa gcagtatcta aaatatactc catatcttct ttaggaatat cattattatt
     8641 ataaacacga atcgccatac gaaaatcaaa agctcttcga acttgttttt tgatatcttc
     8701 ttttgtcatt ttagatcctc ctgttattaa tgctatcata tcacaattta gaaaaaatct
     8761 cagcaggcga tatcgtagca aagtagagct acaaaattct aatgactgtc agaaaaataa
     8821 agggaatgaa caaaatgata gggttttgat gaagaaagtt atataatttt acataaggag
     8881 gggataatac catatatcaa actatcattt agatgtttaa aatagatgtt tttcactatg
     8941 aaattcattt ttgtcacata gaaaaggaga ttgttatgga aacatatact ttatcgaata
     9001 ctcttaacat tcctaaaatt ggttttggga catggcaact aactgaaggt gaagaagctt
     9061 ataaggcagt gactcatgct ttaaaagttg gttatactca tattgatact gctcagattt
     9121 atggtaatga acatagtgtt ggacgtgcta taagagacag tggtttggca agagaaagta
     9181 tctttttgac aactaagatt tggaatgaca aacatgacta tcatttagcc aaagcttcta
     9241 ttgatgaatc tctgcagaaa ttaggtgttg attatattga tcttcttttg attcattggc
     9301 caaatccaaa agctcttcgt gaaaatgatg cgtggaaagc tgggaatgca ggcacttgga
     9361 aagcgatgga agaagcttat aaagaaggaa aggtaaaagc tattggtgtt tcgaatttca
     9421 tgaaacatca cttagaggct ttatttgaaa ctgccgaaat aaaacctatg gttaatcaaa
     9481 ttatcctagc accaggttgt gctcaagagg atttggttcg cttctgtaaa ggaaatgata
     9541 tcttattaga agcttacagt ccatttggaa caggtgctat ttttgaaaat gaatctataa
     9601 aagctattgc tgaaaaatat ggtaaatcag ttgcgcaagt agctcttcgc tggtcacttg
     9661 ataatggctt tttaccatta cctaagtcag cgactcccaa aaatattgaa gcaaaccttg
     9721 atatttttga ttttcaatta aacgaagatg atattgctac cttaatacaa ctagattcag
     9781 gtattaaacc caaagatcca gataatgtct cattttaata agaaaagatg gaaatgtatt
     9841 tccatctttt ttatgtggga tagttcctta aagttaagaa aaccattaag acctccattg
     9901 acgaggagaa tatattaatg atagtattac tagaaaagta gtaataaagg atgagtatgg
     9961 aaaaatcctt tacaatatat cattatcgaa agaggttata acatctaaat gagacttgat
    10021 aaatttttag tagaatgcgg acttggttca cgtactcaag tcaaattaat attgaaaaaa
    10081 aagcagatta gtgttaatgg taatagcgag acatctccaa aagtacaggt agatgaatat
    10141 agagatgaga ttaaatataa tggaactcta gtgtcatatg aaaagtttgt ttattatatg
    10201 cttcataaac ccaaaggtgt tatatcagca acggatgatc cttcgcataa aaccgtgtta
    10261 gatttgctag acaaaactgc tcgagataaa gctgttttcc cagtggggcg tttagatatt
    10321 gatacaacag gccttttgtt attaactaat aatggggaat tagcgcataa aatgttatca
    10381 cctaaaaaac acgttgataa atgctatgag gtaaaaatct cagggataat gacagaagac
    10441 gatattttgg catttgataa agggattatt ctgaaagatt ttacttgttt accagcatta
    10501 ttagaaattg tggaagtaaa tcaggtgaag aagcagagtc ttgttaaaat caccattaag
    10561 gaaggaaaat tccatcaagt taaacgtatg gttgctgcgt gtggtaagga agtcttagag
    10621 ctaaagcgtc taaggatggg aaatctacaa ttagataaac agctagaaag tggacaatgg
    10681 agacggctaa caataaaaga aatagaaaaa ttagaaaaat atatgcaata atataaaaca
    10741 acagttaata ttttagcaat ttgataacac ttagattata atgtaaacgt atactttttt
    10801 agattggaag atcaaatgaa acgtaaatac tttattctta atacggtgac ggttttaacg
    10861 ttagctgctg caatgaatac tagcagtatc tatgctaata gtactgagac aagtgcttca
    10921 gtagttccta ctacaaatac tatcgttcaa actaatgaca gtaatcctac cgcaaaattt
    10981 gtatcagaat caggacaatc tgtaataggt caagtaaaac cagataattc tgcggcgctt
    11041 acaacagttg acacgcctca tcatatttca gctccagatg ctttaaaaac aactcaatca
    11101 agtcctgtcg ttgagagtac ttctactaag ttaactgaag agacttacaa acaaaaagat
    11161 ggtcaagatt tagccaacat ggtgagaagt ggtcaagtta ctagtgagga actcgttaat
    11221 atggcatacg atattattgc taaagaaaac ccatctttaa atgcagtcat tactactaga
    11281 cgccaagaag ctattgaaga ggctagaaaa cttaaagata ccaatcagcc gtttttaggt
    11341 gttcccttgt tagtcaaggg gttagggcac agtattaaag gtggtgaaac caataatggc
    11401 ttgatctatg cagatggaaa aattagcaca tttgacagta gctatgtcaa aaaatataaa
    11461 gatttaggat ttattatttt aggacaaacg aactttccag agtatgggtg gcgtaatata
    11521 acagattcta aattatacgg tctaacgcat aatccttggg atcttgctca taatgctggt
    11581 ggctcttctg gtggaagtgc agcagccatt gctagcggaa tgacgccaat tgctagcggt
    11641 agtgatgctg gtggttctat ccgtattcca tcttcttgga cgggcttggt aggtttaaaa
    11701 ccaacaagag gattggtgag taatgaaaag ccagattcgt atagtacagc agttcatttt
    11761 ccattaacta agtcatctag agacgcagaa acattattaa cttatctaaa gaaaagcgat
    11821 caaacgctag tatcagttaa tgatttaaaa tctttaccaa ttgcttatac tttgaaatca
    11881 ccaatgggaa cagaagttag tcaagatgct aaaaacgcta ttatggacaa cgtcacattc
    11941 ttaagaaaac aaggattcaa agtaacagag atagacttac caattgatgg tagagcatta
    12001 atgcgtgatt attcaacctt ggctattggc atgggaggag ctttttcaac aattgaaaaa
    12061 gacttaaaaa aacatggttt tactaaagaa gacgttgatc ctattacttg ggcagttcat
    12121 gttatttatc aaaattcaga taaggctgaa cttaagaaat ctattatgga agcccaaaaa
    12181 catatggatg attatcgtaa ggcaatggag aagcttcaca agcaatttcc tattttctta
    12241 tcgccaacga ccgcaagttt agcccctcta aatacagatc catatgtaac agaggaagat
    12301 aaaagagcga tttataatat ggaaaacttg agccaagaag aaagaattgc tctctttaat
    12361 cgccagtggg agcctatgtt gcgtagaaca ccttttacac aaattgctaa tatgacagga
    12421 ctcccagcta tcagtatccc gacttactta tctgagtctg gtttacccat agggacgatg
    12481 ttaatggcag gtgcaaacta tgatatggta ttaattaaat ttgcaacttt ctttgaaaaa
    12541 catcatggtt ttaatgttaa atggcaaaga ataatagata aagaagtgaa accatctact
    12601 ggcctaatac agcctactaa ctccctcttt aaagctcatt catcattagt aaatttagaa
    12661 gaaaattcac aagttactca agtatctatc tctaaaaaat ggatgaaatc gtctgttaaa
    12721 aataaaccat ccgtaatggc atatcaaaaa gcacttccta aaacaggtga tacagaatca
    12781 agcctatctc cagttttagt agtaaccctt ttattagctt gttttagctt tgtaacaaaa
    12841 aagaatcaga aaagttgatt gtaatgtaaa ataattccta tttttcactt tttagtatca
    12901 tattttcgat ttttatgttg taataaatta taatgaagat gtaaggaatt ataaaatagg
    12961 aaataatatg aaagtgaaaa ataagatttt aacgatggta gcacttactg tcttaacatg
    13021 tgctacttat tcatcaatcg gttatgctga tacaagtgat aagaatactg acacgagtgt
    13081 cgtgactacg accttatctg aggagaaaag atcagatgaa ctagaccagt ctagtactgg
    13141 ttcttcttct gaaaatgaat cgagttcatc aagtgaacca gaaacaaatc cgtcaactaa
    13201 tccacctaca acagaaccat cgcaaccctc acctagtgaa gagaacaagc ctgatggtag
    13261 aacgaagaca gaaattggca ataataagga tatttctagt ggaacaaaag tattaatttc
    13321 agaagatagt attaagaatt ttagtaaagc aagtagtgat caagaagaag tggatcgcga
    13381 tgaatcatca tcttcaaaag caaatgatgg gaaaaaaggc cacagtaagc ctaaaaagga
    13441 acttcctaaa acaggagata gccactcaga tactgtaata gcatctacgg gagggattat
    13501 tctgttatca ttaagttttt acaataagaa aatgaaactt tattaaatat aaaaaggagg
    13561 actaacctcc tttttatatt taaatagccc attcgccatt tctaaagatt gggacagcag
    13621 ttccatctgc acgaatacca tcaatatcca tttgttctga accaatcata aaatcaacat
    13681 gtgttgaaga acgatttaaa ccagcttcat ccaattcttg acttgtcatt tctgttcctc
    13741 cttctacatt gaaggcatat gcagttccaa tagcgaggtg attagaggca ttttcatcga
    13801 ataaagtatt aaagaaaatc agtccagata gtgaaattgg tgttttatgt gggacaagtg
    13861 caacttcccc aagcgatctt gccccatcat tttcctcgat taagcgttgg actgtttctt
    13921 gccctttttc tgcagtgaca ttgataattt taccgtcttt aaaggtaaat gtcatatttt
    13981 cgataataac gccagcataa ctgagaggtt ttgtacttgt cacataccca tctgcacgac
    14041 ggtaatcagg tgctgaaaag atttcttcag taggcatatt agcgataaaa gtctctccct
    14101 gagcgttgag actaccggct gcctcccaaa tatgattttt aggcatacca agcgttaaat
    14161 ctgtacctgg tgcggtataa tgaagagcat caaattgttc ttgatttagt ctagtagctt
    14221 tttctaataa tttagcttca tgtgcgtccc aagcagcaat aggatcatct tcataaatac
    14281 gatttaattt gaaaatggta tcccaaagag catcaacttg ttggtcgctt gtttttaatt
    14341 ctgggaagac aagtgcagcc cattctctac cagcagctgc agccacgttc caagatactt
    14401 tattagcttg tattgctcta ctttgttcct ctaaggcaat agcggttgct ctagttgttt
    14461 cagataaacg tttaggatca acagtagcga aagcgttagg gttagaagat ttcacaacta
    14521 atcgactagc cttcttattt aatagtgcta aagatttatc aacggtatac tgtggaacat
    14581 tcgtgagaac ttcgtctgaa gcatgaagta gtcgctggcg cgtgataaaa tcatctgtat
    14641 aatcaacaat aacttcactt gctccctttt cataagcggc ttcagttaaa agccttgcta
    14701 agtggacttg ttccacatcg attgttaaag cgagagtgtg ccctttttgg acatttaaac
    14761 ctttagaaat aattaattgg gcatattttt ttaaaaggtt gtcgaaatct tgtaaaacca
    14821 tactagctcc tttaaattta ctatttttta taatttatca taaactctta taaagcacaa
    14881 ggaaatatgg aagcgattta ataaagatat attgatttaa taaagatata tttaagctaa
    14941 cggtttgtag tttgcgaaat aagacgtttc gtgttaatat agacttaact atatattcag
    15001 taaatgaggt gacatgagtg gcttttggag ataacggaca acgaaaaaaa actgggtttg
    15061 aaaaactaac tctttttgtt gttattttga tggtactagt aacagttggt ggtttggtat
    15121 ttggtgctat tagtgcaatc atgtaatgag aaacgctgta aagcgttttt ttatactgaa
    15181 tagaataaag aataggaatt ttagaaatga gtatgttttt agatactgcc aaaattagtg
    15241 ttaaggcagg tcgcggtggt gatggcatgg ttgcttttcg ccgtgagaaa tatgtcccca
    15301 atggaggccc gtggggcggt gatggcggta aaggcggatc ggtcattttt aaagtaaacg
    15361 aaggtttacg aactttgatg gattttcgat ataaccgtaa ttttaaagca aaagctggtg
    15421 aaaaagggat gactaaaggt atgcacggtc gaggtgcaga agaccttatt gtgtcattac
    15481 cacctggaac aactgttcgt gatgctacta caggtaaggt tattaccgac cttgtggagc
    15541 atgatcagga attcgttgta gccagaggtg gccgcggtgg tcgtggaaat attcgctttg
    15601 caaccccacg taatccagct cctgaaattg ctgaaaatgg ggagcccggt gaagaacgtg
    15661 aattacaatt agaattaaaa attttggctg atgttggtct cgttggtttc ccatcagttg
    15721 ggaagtcgac gctacttagt gttgtttcag cagcaaagcc taaaattggt gcctatcatt
    15781 ttacgacaat tgtccctaat ttagggatgg ttcgtacaaa atcaggtgat agctttgcaa
    15841 tggctgatct tcctggtttg attgaagggg ctagtcaagg tgtcggatta ggaactcagt
    15901 ttcttagaca tattgagcgt acacgtgtta ttctacatgt tattgatatg tcggcaagtg
    15961 aaggacgaga cccttacgat gactatgttt caattaataa tgagttggaa acttacaatc
    16021 ttcgattgat ggaacgtccg caaattattg ttgccaataa aatggacatg cctgattcag
    16081 aagagaattt ggcagcattc aaagaaaaat tagcagcaaa ctatgatgag tttgatgaca
    16141 tgcctatgat ttttccaatt tcaagtttag cacatcaagg actagaaaac cttatggatg
    16201 cgacggctga attattagcc aatacagaag agtttcttct ttatgacgaa actgacatgc
    16261 aagaagatga agcatactat ggatttaacg aagatgaacg tccatttgag attacacgtg
    16321 atgatgatgc aacgtgggta ttgtatggtg ataagctaga aaaactcttt gttatgacaa
    16381 atatggaacg cgatgaatca ataatgaaat ttgctcgtca gttacgtggt atgggcgttg
    16441 atgaagccct tcgagagcgc ggagctaagg atggagatat tgttcgtata ggtaacttcg
    16501 aatttgaatt tgtagattaa tgcagtactt gattggaggt tttttatggg agataaaccg
    16561 atatcatttc gtgataaaga tgggaatttt gtttcagcgg ccgatgtctg gaatgctgaa
    16621 aaattagaag aactctttaa cacattaaat cctaatcgta aacttcgttt agaacgcgaa
    16681 aaattagcaa aagaaaaagg atagttaagt aactatcctt tttagttatt ttggtatgaa
    16741 aaaagactct aaaacataat tgatagagtg ttaaggtaca gattacttta ttattttaaa
    16801 cgtttttgag ctttttgata tactgaagag cattttttac aatggttgga gtataagtaa
    16861 taaatttata agcaaaataa aaagcacaaa ctaaagttat aaaaaatagt aagtaaataa
    16921 tagggttttg cgttaagaat ccttgaaaaa ga
//
LOCUS       DATRSD010000049        16795 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRSD010000049 DATRSD010000000
VERSION     DATRSD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.049"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            121..687
                     /locus_tag="VBD85_001287"
     CDS             121..687
                     /locus_tag="VBD85_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7133843.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDVKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(736..1386)
                     /locus_tag="VBD85_001288"
     CDS             complement(736..1386)
                     /locus_tag="VBD85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7133844.1"
                     /translation="MSSVLTLTKILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(1398..2093)
                     /locus_tag="VBD85_001289"
     CDS             complement(1398..2093)
                     /locus_tag="VBD85_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7133845.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHVMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAVRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFVAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(2106..2906)
                     /locus_tag="VBD85_001290"
     CDS             complement(2106..2906)
                     /locus_tag="VBD85_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7133846.1"
                     /translation="MLRRKRLTFYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(2918..3673)
                     /locus_tag="VBD85_001291"
     CDS             complement(2918..3673)
                     /locus_tag="VBD85_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7133847.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPREPRAKQFLSNIINHTSTDL"
     gene            4013..4723
                     /locus_tag="VBD85_001292"
     CDS             4013..4723
                     /locus_tag="VBD85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7133848.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            4716..6065
                     /gene="vicK"
                     /locus_tag="VBD85_001293"
     CDS             4716..6065
                     /gene="vicK"
                     /locus_tag="VBD85_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO7133849.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKNIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            6069..6878
                     /locus_tag="VBD85_001294"
     CDS             6069..6878
                     /locus_tag="VBD85_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7133850.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAG
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            6881..7249
                     /locus_tag="VBD85_001295"
     CDS             6881..7249
                     /locus_tag="VBD85_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO7133851.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            7425..8111
                     /locus_tag="VBD85_001296"
     CDS             7425..8111
                     /locus_tag="VBD85_001296"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO7133852.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            8119..11658
                     /gene="smc"
                     /locus_tag="VBD85_001297"
     CDS             8119..11658
                     /gene="smc"
                     /locus_tag="VBD85_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO7133853.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVRKELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEAKQLALLDVTKLKSDLERQIDLIRLESNQKAEKKEEAGQRLAELEIKAKD
                     CSDQITQKNIELTTLSEKIAQIRSEIVSTESSLERFSTNPDQIIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVATLLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQVNNRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSADLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            11749..12546
                     /locus_tag="VBD85_001298"
     CDS             11749..12546
                     /locus_tag="VBD85_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7133854.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIIFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNNQAVMAYLESMVD"
     gene            12546..13370
                     /locus_tag="VBD85_001299"
     CDS             12546..13370
                     /locus_tag="VBD85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7133855.1"
                     /translation="MSDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKAIAKATTICNNDESGVAEAIGKYILSEEN"
     gene            13370..14980
                     /gene="ftsY"
                     /locus_tag="VBD85_001300"
     CDS             13370..14980
                     /gene="ftsY"
                     /locus_tag="VBD85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO7133856.1"
                     /translation="MGLFDRLFGHKKKDKEPEIEASESVVLEDEDSVIDKEEGSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTDEIPVKEDDSFL
                     ELEDRAKTKVADTSEVENVVPDSTTLSDNVSAKSEASFSDKEQLSDSQASDQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESEFSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEEKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(15017..15829)
                     /locus_tag="VBD85_001301"
     CDS             complement(15017..15829)
                     /locus_tag="VBD85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO7133857.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRVF"
     gene            complement(15829..16731)
                     /locus_tag="VBD85_001302"
     CDS             complement(15829..16731)
                     /locus_tag="VBD85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7133858.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
BASE COUNT         5785 a         2567 c         3329 g         5114 t
ORIGIN      
        1 tgaggtatta atgtcaaatg gttttttatt gaccacatgt ttttataaac gagtggtcaa
       61 aattgaagaa gtatctagct tatgttaaaa tataattgaa taatagaaag aaggtgctgt
      121 atgattaaga aaaataaagt ttttttagga gttcttttag tccttgttgt tatccttgga
      181 ggtggtgtat tattttacca atcacaattc caaaagacaa ctaatcaagc gttagctatt
      241 gcttataaag atgctaaagt ggctaagaag gatgtcatac atcaaaaaat tgacaaagaa
      301 tttgaaaact ttagagggag ctacgaaatt gaattcaata ccaaaagcgc agaatacagc
      361 tatcatgtag atgttaagac tggacaaatc cttgagcggg acatggataa taatggcttt
      421 tcaaaatcga cttctcagtc aagttcatcg tcaagtcaaa aatctcataa aataagtcaa
      481 gaagaagcca aaaagattgc atttaaagat gctaatatag aggaatcaga agtcagtaat
      541 cttaagatca aagaagaaat tgaaaatgga aaatcagttt atgatattga ctttgtggac
      601 ctgaaaaata aaaatgaagt tgattatcaa attgatgcag aaactggaaa aatcattgaa
      661 cgttctagag atcatatgaa tgattaattt aaataagata taaaaaaacg aaggtctaaa
      721 aggccttcgt tttaattact cagtagcttg ctcccaacgt tttgaaagtt tacgtgaaaa
      781 acttgagatg ataaagtttg taatgaaata gacagcagtt ataatagcgt agagtgtaaa
      841 aacttgtcca gcttcgaaat atcttcccat tagaatttga ctttttccga agatttcttg
      901 aatagctata acagaataaa gtagtgatgt atcttttata accgttacaa actgagaaat
      961 aatggctggc aacattttgc gaaaagcttg tgggaaaata ataataatga aaacttgcaa
     1021 ataagtgaat ccttgagaaa gtccagcttc tgtttgtcct ttatcgactc catttaaacc
     1081 tccccgaata atttctgcta aagcagcgga ggtaaaaatt gtaaacgaag taataccagc
     1141 actgactgat ttcatctgga atactaagaa aatgataaaa atccaaagta gatttggaac
     1201 atttctgaca aattcgatat agatactagc tattaatttc cagataggat ttttgctatt
     1261 tctcataatt gctaatagag ttccaaaaaa cattgatagt aaaatggaaa taaaagatat
     1321 atataaggtt aaaccgaacc cttgaaggag aaatagtatc tttgttagtg ttaagactga
     1381 tgacatatca aacctcctta agaataagtt ttcttattta attcttcttg ttttcttgcc
     1441 cagcttgcta gtgggaaaca aataacaaaa tagaagatag cagctcctgc aaaagctggg
     1501 atataattag tcgtatcata agcccaggcc ttagctacaa acatgatatc ggctccagaa
     1561 atgattgcta cggtagaggt attcttgatt agattaacaa cttggttggt cattggtggt
     1621 aaaattgttc tgacagcttg aggcaaaatg ataagttgca tggtttgatt agctgtgaat
     1681 ccttgagcta aagcagcctc tgtttgtcct ttagggacag cttcaattcc tgagcgaata
     1741 acttcagaaa tataggcacc atggtatagt ccaacgcaca aaacggcggt gaaaaaagca
     1801 ctaatcataa catgtccatt actaatgatt gctagtccat agtagacaaa aaccatttga
     1861 actaaaagag gtgtgttttg aaaaacttca acatagatac gtgatatcaa cttaccaact
     1921 ttacttttag aagttgataa accaccagag agaattccta atatcagagc aagtaggagt
     1981 gcaccaaacg aaatacctag agtatataag aagcctttaa agaataggtc gaaatggtta
     2041 aagaaagcgc cccaccttga aatggcaaaa ggactgatgt ttaataaagt cataaaaaat
     2101 cctccctatt ctttttttac tttagcgggt ttaagcttaa atttttgata aatgtgtttt
     2161 aaactaccat ctttttccca tgataccagt aacttattaa cgtagggaat caagactttg
     2221 tcctgtttac tggttgcaat accgtattct tgcgtattga acccttcttt taatatggtt
     2281 gtatgagggc taaggtagcc gctaaggata gatttatcaa ctgaaaaagc atcaatacga
     2341 ttagcgtaca gcgatatcgc taattctggg aaactcccta attgagcata gctaaatttt
     2401 agcttgtgtt ctttagcata tttttctaaa ttgacttttg tagtggcacc ttgagccaca
     2461 ccaatgtgtt taccatccaa ttcttttata gttttgatat gactattttt acgcaccaaa
     2521 aatccaattt catcgtgata ataagcttta gaaatattat agttagcttt tctttcgggc
     2581 gtgatagtgt atgttgcgat tagaatatca atttgcccat tgtccatcag aggctctctg
     2641 gtttgtgcgg ttgtcggtac aaaaactggt ttgacaccta aggatttggc aatttttttg
     2701 gcaatatcaa tttccattcc ttcgtattga ttcgtctcag cattataata accaaagtta
     2761 ggcacatctt gtttgactcc aactttaagg acgcccgatt ttttgatttc tgataattga
     2821 ttagctgacg ttgagttagg ataaaataac aggaaaataa aaatacagga taatagatag
     2881 aacgttaatc tttttcgtct cagcatattt cgtcctccta aaggtcagta ctggtatggt
     2941 tgataatgtt gcttaaaaat tgtttagcac gtggttctct gggattatca aaaaagtctt
     3001 gtacatctgt agtatccact aaaatttccc catcagccat aaaaataata cgatcagcaa
     3061 cttctcgtgc aaatcccatt tcatgagtta caacgaccat attcattccg tcatttgcca
     3121 atttttgcat cactgaaaga acgtctccga tggtttcggg atcaagggca gaggtaggct
     3181 catcaaagag gaggagtttt ggatgcatgg caagccctct agcaatagca attctttgtt
     3241 tttgaccacc tgatagcata gatgggtagg aatcttttct ttcccacata ttgacaaatt
     3301 ttaaatattt ttcagcaatt tccatagctt cttttttaga ttgttttaat actttaatag
     3361 gagctaatgt aatattttct aacacggttt tgtgaggata aaggttaaaa tgttgaaaaa
     3421 ccataccaac ttctttacgg aggttgacca attctttaga tgaaatattt gctaattcat
     3481 gtccattcac gaccaaacta ccatcgtcaa tagattcgag agcgttcatt gttctaataa
     3541 gtgttgattt acctgatcca gaaggtccta atagaacaac cacttgccct ggctctattt
     3601 ccaaattaat ctgacgaagg gcgtgataat ctccataata cttgttaaca ttcttataag
     3661 atatgagtga cataagaacc tcctaaaata acagtttatt gttaggttac ctaacattat
     3721 agataagtgt atcacctgtt tgtgagcttg tcaataaatt cagaatatta aagagtagga
     3781 ttgatttaaa tattgctatt tttagagcgg tatttttagt aaaaatatta cgttctcgta
     3841 tcatttattt ttcagtttaa taggatgtat tgaaagtttc caattaacta ggaaatctgt
     3901 tataattagt gataatagtg gtgctttagg cccatattat catattttcg gtcactttta
     3961 tttttcagtt tatgaatgga agtgggagac cagaaagtaa tagaggtaaa atatgaaaaa
     4021 aatacttatc gttgatgatg aaaaaccaat ttcagatatt atcaaattta atttaaccaa
     4081 agaaggatat gagacggcga cagctttcga tggccgtgaa gctttagtac aatatgcaga
     4141 atttcaacca gatttaatca ttttggattt aatgcttcct gaactagatg gtttagaagt
     4201 ggctaaggaa gttcgtaaaa ccagccatat tcctattatt atgctttctg caaaagacag
     4261 tgagtttgac aaagtcattg gtttagaaat cggtgcagat gattatgtta ctaaaccttt
     4321 ttcaaatcgt gaacttttgg cacgggtcaa agcacattta cgacgtactg aaaatattga
     4381 aacagctgta gcagaagaaa gtgctcaaaa tgcttcttca gatattacta ttggtgagtt
     4441 gcaaatttta ccagatgcct ttattgctaa gaaacgtggc gaagaaattg aattaacgca
     4501 ccgagaattt gaattacttc accatttagc gacacatatc ggacaagtca tgacacgcga
     4561 acatttattg gaaacagttt ggggatatga ttattttgga gatgtccgta cagttgatgt
     4621 gacggtgaga cgtttacgtg aaaagattga agatactcct ggtcgtccag aatatatttt
     4681 gactcgacgt ggtgtaggtt attacatgaa gtcttatgaa taatagtgct gcaaatatta
     4741 ggtcgtttga gctagcctta ctttttttac tagtttttgt tgctgtttat tttgtttatc
     4801 tagcggttag agattttaaa atgtctaaaa atatccggct tttgaattgg aaagtcagag
     4861 atttgatagc aggaaattat tcagatagta ttctaattca aggtgatgca gatttggtag
     4921 aattaggtga aagtctaaat gatttatctg atgtcttccg aatggcacat gataacttag
     4981 aacaagaaaa gaatcgctta gcaagtatct taacttatat gactgacggt gtgctggcta
     5041 cagatcgttc aggaaaaatc gttatgatta atgagacagc ccaacagcag ttcaatctag
     5101 catatgatga agctttatca atgaatattg ttgatatgtt agggtcggga agtccatatt
     5161 cgttccaaga tttagtctct aaaacgccag aggtagttct taatagacgt gatgaaaacg
     5221 gtgaatttgt taccttacgg attcgatttg ctttgaaccg tcgtgaaagt ggttttattt
     5281 ccggtttagt tgcagtgtca cacgatgcta ctgagcaaga gaaagaagag cgtgaaagaa
     5341 gactttttgt atctaatgtt tctcatgaac ttcgtacacc tttgacatct gtaaaatctt
     5401 atttagaagc tttagatgag ggagcgctta atgaagaagt tgctccaagc ttcattaagg
     5461 tatctctaga tgaaacaaat cgtatgatgc gtatgatatc agatttatta agtttatcgc
     5521 gcattgataa tgaagtaacg catttagatg ttgaaatgac gaattttaca gctttcatga
     5581 cctcaatttt gaatcgattt gatcagatta gaaatcaaaa aacagtcaca ggaaaagttt
     5641 atgaaattgt cagagattat cctcttaagt caatttgggt ggaaattgat acagataaga
     5701 tgactcaagt gattgataac attttaaata atgcagtcaa gtattcacca gatggtggta
     5761 agatcacagt taatctacgc acaactaaaa cgcagatgat tttatcaata tcagaccaag
     5821 gcttaggtat tcccaaaaaa gatttacctc tcatctttga tcgtttttat cgtgttgata
     5881 aggcgagaag tcgtcaacag ggtgggactg gacttggttt gtcaattgca aaagaaattg
     5941 ttaagcagca taagggattt atttgggcta agagtgagta tggtaaaggg tctactttta
     6001 caatagtctt gccttatgat aaagatgctg taacttatga agaatgggag gacgttgaag
     6061 attaaaacat gtcagaaata ggatttaaat atagtatctt agcttcaggt tcaactggaa
     6121 attgttttta tattgagaca ccccaaaaac gtcttttaat tgacgctggt ttaacaggca
     6181 agaaagtgac tagccttctt gcagaaatta atcgtaaacc agaagattta gatgctatac
     6241 tagttaccca tgaacattct gaccacatta aaggtgttgg cgtattggct aggaaatatc
     6301 accttgatat ttatgccaat gagcagacgt ggaaagttat ggatgagcgt aacatgttag
     6361 gcaaagttga tgttagtcaa aagcatgttt ttggacgcgg taaaacctta actttcgggg
     6421 atttagatat tgagagcttt ggtgtaagtc atgatgctgt ggatccccaa ttttaccgta
     6481 tgatgaaaga tgataagagc tttgtgatgt tgacagatac aggatatgtt agcgaccgaa
     6541 tggcaggctt gattgagaat gcagatggtt atttgatcga gtcaaatcac gacattgaga
     6601 ttctacgttc gggttcttat ccttggacac ttaagcaacg tattttatca gataaaggac
     6661 atttatctaa tgaagatggt tcagaaacca tgattcgcac tataggtaac cgtactaaac
     6721 acatctattt aggacacttg agtaaggaaa ataatattaa ggaacttgct cacatgacta
     6781 tggaaaacaa tttaatgaga gctgactttg gtgtgggcac agatttttct gttcacgata
     6841 cttcacctga ttcagctacc ccactgacga ggatttaaga ttgaaaaaga ttttttacat
     6901 tagtttagga tttattagtt tagggatagg cattgcaggt attgtcttgc cggtagttcc
     6961 aacgactcca ttagtattgc tttcagcttt ttgcttttcg agaagctctg aaaaatttga
     7021 tatttggttg cgtcaaacta aagtttataa atactacgct gcagattttg ttgaaagcag
     7081 aagtattgcc ccagcgcgta aaaagtcaat gatttggcaa atttatatct tgatgggaat
     7141 ttcaatctat tttgctcctc taatgtggct caaactggga ttattgattg gaactattgt
     7201 aggaacttat gtgctttttt atgtagtacc tgataaaatt gatgaatagt taacaagaga
     7261 ctaggtgtgt atcctagtct tttgttgaat attcaaatat gtaattaagg aaacgttttc
     7321 tttggaagat aggtttaact tccaaaatgt ttgattattt gtcaactgtg gtataatggt
     7381 atttgtttaa tattttatct atgattgaaa tcaaggagaa aaaaatgaaa gaattaagat
     7441 caaagctgga aaaagactat ggtattgttt ttgctaatca agaattatta gatacagctt
     7501 ttacccatac atcatacgca aatgagcatc gcctcctaaa catttcacat aatgaacgtt
     7561 tggaattttt aggagacgct gttctacaat tacttatttc acagtatctt tttacaaagt
     7621 accctcaaaa ggcagaaggt gatttatcaa aattacgttc aatgattgtt cgtgaggaaa
     7681 gtcttgcagg tttttcacgt ctatgtggct ttgatcacta tattaaactt ggaaaaggag
     7741 aagaaaaatc tggtggacgt aatcgtgaca ctattttagg agatttattt gaggcttttt
     7801 taggagcttt attattagat aaaggtgttg aagtggttca tgcctttgtt aacaaggtaa
     7861 tgatccctca cgttgaaaaa gggacctatg agcgtgttaa agattacaag acaagcttgc
     7921 aagaattact tcaatctcat ggggatgtaa aaatagatta tcaagttact aatgaaagtg
     7981 gtccagcaca tgcaaaggaa tttgaggtga ctgtatctgt caaccaagaa aatttgagcc
     8041 aaggaattgg acgatcaaag aaagctgctg aacaagacgc tgccaaaaat gctttagcaa
     8101 cgttacaatg aggaaatcat gtttttaaaa gaaattgaaa tgcaaggctt taagtccttt
     8161 gcggataaaa ctaaggtaga atttgatcaa ggagttactg ctgttgtagg tccaaatgga
     8221 tcaggtaaaa gtaatatcac agagagttta cgttgggcat tgggagaatc aagcgcaaaa
     8281 agtttacgag gaggtaagat gccagacgtt atatttgctg gtacagaaaa tcgtaagccc
     8341 cttaattatg cgcaagtgag tgttacttta gataatagcg accattttat cgaaaatata
     8401 gctgacgaag ttcgagttga acgtcgtatt tttcgaaacg gagatagtga atatttaata
     8461 gatggacgca aagttcgctt acgtgatatt catgatttat ttatggatac tggattagga
     8521 agagattctt tttctattat ttcccaagga cgcgttgaag ctatttttaa tagtaaacca
     8581 gaagagcgcc gtgccatatt tgaagaagcc gctggtgttc tgaaatacaa aacacgtaag
     8641 aaagaaacac aatccaaatt agaacaaact caagggaact tagatcgctt agaggatatt
     8701 atttatgaat tggacatgca agtccaacca ttagaaaaac aagcaagtat tgccaagcgt
     8761 tttttagtat tagatgagga acgacaagga ctccacttat ctattttgat tgaagatatt
     8821 ttacaacatc aatcagattt aactactgtt gaggaaaagc tattgacagt aagaaaagag
     8881 ttagcaactt attaccaaca acgtcagtcc cttgaagatg aaaatcaatc gttaaaacag
     8941 aaacgtcacc atttatctga agaaattgag gctaaacagt tagctctttt agacgttaca
     9001 aagttaaagt cagatttgga acgccaaatt gatctgattc gtttagagtc aaaccaaaag
     9061 gctgaaaaaa aagaagaggc aggtcaacgc ttagcagagt tagaaattaa agctaaagat
     9121 tgtagtgacc agattaccca aaaaaatata gagttaacta ctctgtcgga aaaaatagcc
     9181 caaataagat cagaaattgt tagtacagaa agttccttgg agcgtttttc aactaatccg
     9241 gatcagatta ttgaaaaact tcgagaagac tttgttacct tgatgcaaga agaagccgac
     9301 acttcaaacg ctttaacagc tttactagct gatatagaga atcaaaaaca agcttctcaa
     9361 gctaaatctc aagaaataca agaagttagc aaaaatttag aagtattaaa aagtaacgca
     9421 aaggtagcat tggaacgatt tgaggctgca aagaagaatg ttcgtcagtt attatctcac
     9481 taccaagatt taggtcaaac cttgcaaaat ctagaaggtg agtataagaa tcagcagtca
     9541 attttatttg atcatttaga tgaaatcaaa tcaaagcaag cacgtatttc gagtttagaa
     9601 tctattttga aaaatcatag taatttctat gctggagtga agtcagttct acaggctaag
     9661 gaccagcttg gaggtatcat aggtgctgtt agtgagcatc ttagctttga caaacattac
     9721 caaacagcat tggagatagc tttgggaggt agcagccaac acatcattgt tgaagacgag
     9781 agtgctgcca aacgttcaat tgctttttta aagaaaaacc gccaaggtag agcgacattt
     9841 ctgcctttaa ctacaataaa accaagagaa ttagcacaac attatctctc aaaattacaa
     9901 agttctcaag ggtttttagg aatagctagt gaattggtaa cctatgatca acgcttgtca
     9961 aacattttta aaaataactt agggttaact gctatttttg atacggttga taatgctaat
    10021 gtagctgcga gacaattgaa ttatcaagta cgtttagtaa ccttagatgg aacagagtta
    10081 cgccctggag ggtcgtactc agggggagca aatcgtcaaa ataatactgt ttttatcaag
    10141 cctgaattgg ataatctaaa aaaagaattg aaacaggcac aaagtaaaca attaattcaa
    10201 gaaaaagaag ttgccacatt gttagagcaa ctcaaagaga aacaagaaac cttagcacaa
    10261 cttaagaatg atggagaaca agctcgtctt gaggaacaaa gagctgatat agagtatcaa
    10321 caactatcag aaaagcttgc tgatttaaat aaactatata atggcttgca attaagcagc
    10381 ggggcgttgg aacaaactac ttctgaaaat gaaaaaaatc gtttagaaaa agaacttgaa
    10441 caatttgcta ttaaaaaaga agagttgaca acttcaattg ctcaaattaa ggaagataaa
    10501 gattcaattc aagaaaaagt caataatcta acaacattat tgtcagaggc acagctagaa
    10561 gaacgtgatt tgctaaacga gcagaaattt gaacgtgcca actgtacacg tctagagatt
    10621 accttgtctg aaataaaacg tgacattagt aatttacaaa cattactaag ccatcaggac
    10681 agtcaattag acaaagaaga gcttccacgt atagaaaaac agttgctaca agtaaataat
    10741 agaagagaaa atgacgaaga aaagcttgtg agtctccgtt ttgaattgga ggattgtgaa
    10801 gctgctttag atgatttagc tgcttctctg gctaaagagg gacaaaaaaa tgaaagtctt
    10861 attcgtcagc aagctcaatt agagtcacaa tgtgaacaat tatctcaaca attgatgata
    10921 ttctcacgtc aattatcaga agactatcag atgacattgg atgaagcgaa ggtaaaagca
    10981 aatgtcttgg aagatatttt gatggcgcgt gaacaattga aatctctaca agctaaaatt
    11041 aaagctttag gacctgttaa tattgatgca attgctcaat ttgaagaagt tcatgaacga
    11101 ttgaccttcc ttaacacaca gcgagacgac ttagttcatg ccaaaaattt attactagag
    11161 acgattacag atatggatga tgaagttaag actcgtttta aatcgacatt tgaagccatt
    11221 cgtcatagtt tcaaagaaac ctttgtacag atgtttggtg gaggttctgc ggaccttatt
    11281 ctgacagaag gtgatttatt atcagctggg gttgatattt cggttcaacc tcctggtaaa
    11341 aaaatccagt ctttaaactt gatgtcaggt ggtgaaaaag cattatcagc gttagcatta
    11401 ttatttgcca ttatccgtgt taagacaatt ccatttgtca tcttggatga agttgaggcc
    11461 gctttagatg aagcaaatgt taagcgattt ggcgattatc ttaatcgatt tgataaatca
    11521 agtcaattta tcgttgttac tcaccgaaaa gggactatgt ctgctgctga tagtatctat
    11581 ggggtaacga tgcaagaatc aggcgtttcc aaaattgttt ctgtaaaatt aaaagaagct
    11641 caagaaatga ccaattagaa aacagaaggg gatcccttct gttttttgga ttttcagcct
    11701 tattttatgt tacaatagta aatgtaaacc ctttagttag gagattccat gataaaatta
    11761 gtagcaaccg atatggacgg tacattctta gatgaaaacg gtacctatga taaaaaacgt
    11821 ttagcaaatg tgctaaaaaa atttaaagaa caaggaattg tctttacggc agcaagtggg
    11881 cgttccttat tatcacttga acaattattt gctgactttc gtgatcagat ggcttttata
    11941 gcagaaaacg gaagtgcagc agtcttattt aatcgcttgg catatgaaca gcacttgagt
    12001 cgagaacaat acttagatat tattgaccat ttgagtaaga gcccttatat ggagaataat
    12061 gaatatgtct tatctggcaa ggatggtgca tatattctaa gtgatgccaa tccagattat
    12121 attgaattca tcactcatta ttatgataat ttgcaaaaag tttctcactt tgaagatgtt
    12181 gatgatatta ttttcaaagt aacggctaac tttactgaag agactgttag acaagctgag
    12241 gagtgggtaa atcaagctat accgtatgcc acagcagtga caacaggttt taaatccatt
    12301 gatattattc taagctcagt taataaacga aatggtttag agcacctctg tgaacaatac
    12361 ggtattagag cagaagaagt cttatctttt ggtgacaata ttaatgattt agaaatgcta
    12421 gagtggtcag gaaaagctat cgcaacagaa aatgctagac cagaagttaa agagatagca
    12481 gattgtatta taggtcatca caataatcaa gctgtaatgg cttatttaga aagtatggtg
    12541 gattaatgtc ggatattaaa atacttgcct tagatttaga tggcacttta tttacaacag
    12601 ataagaaagt ttcagaagaa aataaagtgg ccttaaaagc agctagagaa aaaggtataa
    12661 aagttgttat tacaacaggg cgtcctttaa aagcaattgg taatttgtta gaggacctag
    12721 agttagtatc tgatgaagat tacagcatta cttttaacgg tggtttagtt caacaaaata
    12781 cagggaaaat cttagctaaa actgcaatga cacgacaaga agtggaagat atccatgagg
    12841 agttgtatca agtgggtttg ccaacagata tcttaagtga gggaacggta tatagtatcg
    12901 ctaacaaggg acaccattct caatatcatt tagccaaccc gctcttagaa tttatagaag
    12961 ttgatgattt agagcaagtg ccaaaggatg tagtttataa taaaattgtt agtgttattg
    13021 atgctacata tcttgatcaa caaattgcta aacttcctga tagacttaaa gttgactatg
    13081 aaatgtttaa atcacgtgat atcattttag aattgatgcc taaaggtgtt cataaagcag
    13141 tcggcttaga actactgaca aaacaccttg ggttagatag tagtcaagtt atggcaatgg
    13201 gagatgaagc aaatgattta tcaatgctag agtgggcagg cttaggagtt gcaatggcta
    13261 atggtattcc agaagctaag gcaatagcaa aagcgacaac tatttgtaat aacgatgagt
    13321 ctggagtcgc cgaagcaatt gggaaatata ttttaagtga ggaaaattaa tgggattatt
    13381 tgaccgccta tttggacata agaaaaaaga taaagaacca gaaattgaag catcagaatc
    13441 tgttgtttta gaagatgaag attcagttat tgataaagag gagggcagca acttttcgaa
    13501 ggagtcaact ttgaatcgaa cctctgaagt ccctgttgca gaagatgatt ctttcttaga
    13561 attagaaaga gatacagcat tgtcagaatc acatcaacca gttacctctg aaattcatcc
    13621 tttagagtca gaagataccg atgaaattcc tgtaaaagaa gacgatagtt ttttagaact
    13681 agaagatagg gctaagacaa aggtagcaga tacttctgag gtagaaaatg tagttcctga
    13741 ttccacgact ctttctgaca atgtttctgc taaatctgag gcatctttta gtgataagga
    13801 gcaattaagt gatagtcagg cttcagatca gttttcagaa actccccttc aagaagagat
    13861 gtctagtgga aaaactgagg tacaaacaga gtcagaggat acatctgcgg ctgatgcttt
    13921 tttagcagat tactatgcta aacgtaaagc aattgagaaa gaaatctcgt ctaattcgct
    13981 gtcaacagat gagtcagaat tttctgaggc acaggaagtt ttatcgcaat cacaggcaga
    14041 cactataaaa gctgagagtc aagaggaaaa atataatcgt agcctgaaaa aaacacgcac
    14101 aggttttagt gcacgcctta atgctttcct ttctaacttc cgtcgtgttg acgaagagtt
    14161 ctttgaagaa ttagaagaaa tgttaatctt atctgacgtt ggggtaaatg ttgcaacaca
    14221 gttaaccgaa gatcttcgtt atgaagcaaa attagagaat gccaagaaat cagaggattt
    14281 aaaacgtgtt atcgttgaaa aattagttga aatctacgaa aaagatggta tttataatga
    14341 ggctatcaat ttccaagaag gtttaaccgt aatgcttttt gtaggtgtta atggtgttgg
    14401 aaaaacgaca tctataggta agttagctca tcaatacaag tctcaaggga aaaaagttat
    14461 gttagtagca gctgatactt tccgtgcagg tgctgtggct caactagtag aatggggacg
    14521 ccgtgttgat gtaccagttg tcactggaga agaaaaagca gatccggcta gcgttgtctt
    14581 tgatggcatg gaaaaagccg ttgctcaagg cgttgatgtc ttgttaattg atacagcagg
    14641 acgtctgcaa aataaagaaa accttatggc agagttggaa aaaattggac gtattattaa
    14701 gcgtgtcgta cctgatgctc cccacgaaac tttgcttgct ttagatgcat ctacaggaca
    14761 aaatgcttta agtcaagcta aagagttctc gaaaattaca ccattaactg gccttattct
    14821 gactaaaatt gatggtactg ctaagggtgg tgtggtactg gctattcgtc aagaactaga
    14881 tattcctgtt aaattcattg gtttcggcga aaaaatcgat gatattggtg aatttaattc
    14941 agaagatttc atgcgtggcc ttctggaagg aattttataa gaaaaaagaa ctttcattta
    15001 gaaagttctt tttatattaa aagacacgat aaacataagg attattaggt ttagaaactt
    15061 gacgagactc ttcaatgtgt aagactggta agttttgttg taacaattga ttatactcgg
    15121 atttaattgt acctctaaca gttacccaag tattgtccgg ataacttttt tgatttccag
    15181 tcgttagtag accataaact cctgaatcgg caatacaatg aatgattcca aagcggaaaa
    15241 taaattgata tccttcgtgt ttgggttcgt tataaacaaa cccagtatat tgtatttggc
    15301 gatccatgaa ttgctcggga tagagataaa ttagttccat aacttccatg taattttcag
    15361 ttgtgatggt taatgtacca gagcctttat attttttaag ctctttttgc atttcttttt
    15421 cataggctga agaagtaaaa taagtgctgg tatctggttt caaatattga acgcgtgtcc
    15481 catcttgact tacagtacca gtagagccgg ctgctagagg gaaattatat cccttggcag
    15541 agactgtggt tgagtctaaa ctaactgtag gtactaacaa acctactaaa acgggaaata
    15601 ctaaaatcat tgggcttgtt gatttagcta ttttaccatg taggtgacta tgcattttca
    15661 tatttttaac ccaaataatt agctgtacaa ttgctaaaat aaaagatagg accattgaaa
    15721 tataggccaa ataggtataa tgagtattaa tatactgatt tagtttacct gataacttta
    15781 aatacatgct taattcaaaa taaccagcaa gaataagaaa tcgaatcatt atattacccc
    15841 cacaaataaa caatagataa taataataag agaagataca ctaatgaatt ggacaataaa
    15901 ccttgtttta aaagaattta ccatcatcat taaattctta atatcaatca tgggtccaat
    15961 taaaaggaaa gccatcactg gcgcgattcc aaaggttgat aataaagaag cacctataaa
    16021 agcgtcagct tcactacaca atgacaagat gaaagcaagc aacatcataa ctaagatggc
    16081 tgtgataggg ctatgtccga tagtagtaag aactcgagtt ggtaaataaa tttgcatagc
    16141 tgaagcaatt aaagtaccaa atactagata gcgtcctgta tcaaaaaatt catcgatggc
    16201 atgagctaag gctagaaaga ttttttgata ccacttctta tcagaataat catgaaaatg
    16261 agtaggttta gcgtcttctt ttagaatatt atcatctact aaaaaggcaa gcatgactcc
    16321 taaagcaatg gctacaatgg tagcacctac aaagcgtaat atcaggaagc gtatggaatt
    16381 tccaaatgct gagtaggtcg caaataaaac aataggatta ataatagggg cagtagcaag
    16441 aaacggaact gccgtgtatg aaggcacttt cttttcaagg aatcgattaa taataggaat
    16501 gataccacat tcgcaagaag gaaagacaaa gccaacgaag gttccaaata agactctgag
    16561 aaatttattt ttcggtaaga acttattgac tatatcaggt gttataaaga cttcaataat
    16621 tcctgataaa atagttccaa gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa
    16681 aatggcaaac cattgaagga cactatccgg taattgatta aagatatcca tagaacttaa
    16741 tctttctgag aatcatcttt ctgttcaggt tttgaatcag aatcaacttt tttag
//
LOCUS       DATRSD010000050        16607 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRSD010000050 DATRSD010000000
VERSION     DATRSD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.051"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(3..1607)
                     /locus_tag="VBD85_001303"
     CDS             complement(3..1607)
                     /locus_tag="VBD85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133859.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(1611..2345)
                     /locus_tag="VBD85_001304"
     CDS             complement(1611..2345)
                     /locus_tag="VBD85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133860.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSVKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMKYPDKDLETIYLELAGRQASREG"
     gene            complement(2365..2670)
                     /locus_tag="VBD85_001305"
     CDS             complement(2365..2670)
                     /locus_tag="VBD85_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7133861.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            complement(2883..4868)
                     /gene="tkt"
                     /locus_tag="VBD85_001306"
     CDS             complement(2883..4868)
                     /gene="tkt"
                     /locus_tag="VBD85_001306"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7133862.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVADRFASDLQARGAKAEEAWNDLFAKYEVEYPELATEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            complement(4993..6423)
                     /locus_tag="VBD85_001307"
     CDS             complement(4993..6423)
                     /locus_tag="VBD85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7133863.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAHQNLGLWFSICHHRLLAMEK
                     ISDNLKPIVKHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHHISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSHSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            complement(6414..7751)
                     /locus_tag="VBD85_001308"
     CDS             complement(6414..7751)
                     /locus_tag="VBD85_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7133864.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDAVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGFYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(7839..8537)
                     /locus_tag="VBD85_001309"
     CDS             complement(7839..8537)
                     /locus_tag="VBD85_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7133865.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENRADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            complement(8549..10378)
                     /gene="glpO"
                     /locus_tag="VBD85_001310"
     CDS             complement(8549..10378)
                     /gene="glpO"
                     /locus_tag="VBD85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO7133866.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNISQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(10391..11899)
                     /gene="glpK"
                     /locus_tag="VBD85_001311"
     CDS             complement(10391..11899)
                     /gene="glpK"
                     /locus_tag="VBD85_001311"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO7133867.1"
                     /translation="MSSEEKYIMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(12014..12277)
                     /locus_tag="VBD85_001312"
     CDS             complement(12014..12277)
                     /locus_tag="VBD85_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO7133868.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLMIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(12366..12623)
                     /locus_tag="VBD85_001313"
     CDS             complement(12366..12623)
                     /locus_tag="VBD85_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO7133869.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(12635..14674)
                     /locus_tag="VBD85_001314"
     CDS             complement(12635..14674)
                     /locus_tag="VBD85_001314"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO7133870.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVLVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(14678..15319)
                     /locus_tag="VBD85_001315"
     CDS             complement(14678..15319)
                     /locus_tag="VBD85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7133871.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(15319..16233)
                     /gene="glyQ"
                     /locus_tag="VBD85_001316"
     CDS             complement(15319..16233)
                     /gene="glyQ"
                     /locus_tag="VBD85_001316"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO7133872.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
BASE COUNT         5334 a         3431 c         2611 g         5231 t
ORIGIN      
        1 tactaaacaa gcttcttcca aaaatttttt agcagtaagt attggcaaat tgataatatg
       61 agcaacccaa tgataaggat tatgatattg acagctacag tattccatac cattgaccaa
      121 aaaatactta ttccaataag aaaactacct attatcatca ttccaaacaa tgaaccaact
      181 aacagccatt ggcctaaccc tctattaaat agttgcgtaa cctcttgcca attcaagaac
      241 aaatgtttat agtcacgtct ataaataaac tggccttcga taagtcccat gaagactaga
      301 cctaaaatga agctcaataa aacgaggatt gatagtttaa gtagaaagaa tcctaccaag
      361 aaataaataa aagtaggaac tgcaagttgt aaggttatca aagttacaaa tttatgcttc
      421 aagaaatacg aaaatgatat aggcaaactt tttataaagt agaaattact tttttctaaa
      481 gaaataccta cccctacaat gctagctggc atcacacata aactacctat taatattcct
      541 gctagaaaag ctatcccaaa ataattttca ttaaaaaatc gaccaatctc tttaccatta
      601 ttcaaaattg gaacaatgaa cattgctaga tataatagtg gcattaaaaa tgtatttgtc
      661 aataatgtcg cattttgtaa acttgataaa tggtgtttaa caagagtttt attaattgac
      721 tcacgtttac taaaatcgat ttgacgatgg acttttctct gcttgacctg tccatacaac
      781 aactcctgat agtagcgtgg cataacccat ttataaatag cacctatcaa gaataaaatg
      841 attaagagcg ggagataata gttgaggaga ctctccatac taaatggcgc tgtaacaata
      901 tcataataac ctctgacata agggattgga gcgatatctt gtaatttccc ttgtaccatc
      961 ttttgactcg tcatattcac aaataataag ggaactattg ctccaaatgt agctagtgaa
     1021 attaatatag tagaaataag tttccgatta gcacttcgaa taatggcacg gcctattaaa
     1081 ctattaatat acaatgaaat caccgttacc gataaaaata aaatagcaaa atttatgatt
     1141 gctaggagga ccgcaagtgg gccacctatt atacgccaat aagccacaat cattaaggtt
     1201 aggattggca ttagaaaagt tacagacatc ccaaatgttg caaaaatttt agcaacatag
     1261 agctcttcag atttaatagg caaataagca tattgtttaa catcgtcact ctcatagaaa
     1321 acactgtaca tcgcaataaa agagtagata atggatacta gtgtaaagat accaatgtag
     1381 aacgtgaagt agcccggata ttctttaaaa tcaagtgata caaagagaaa tacataaatt
     1441 attgaaaaga gcaaaatctg aaataactga tttctaaaca tggatttata agctgaaaat
     1501 tcttttttag gatgtttttc ttgctttttt cttagtgccg atagagtctg agggtttgaa
     1561 taaaggatat taatttttac gagttcccag atacgtgacc aattcatact ttatccctcc
     1621 ctactagctt gacgtcctgc taattccaaa tagatagttt ccaaatcttt atctggatat
     1681 ttcattttta actcacctaa gctaccgaca aagattaatt tcccttgttt caaaatacct
     1741 attctgtcgc ataactgttc tgcaactgct aagacatgtg ttgaaaaaat aactgttttt
     1801 ccatttttag catgttcttt catcatttct ttcaaatcaa aagaagcttg agggtctaaa
     1861 ccagttaacg gctcatctag aatccaaatg tcaggatttg gaagaagggc acctataaca
     1921 atgacttttt gacgcatacc atgtgaaaaa ctttcaatag gattatagcg ttgctcctct
     1981 agttcaaata tgtctaccaa ttttgtgata cgtgcttcaa tatcttctgg ggcaacgtca
     2041 taaattttag ctaaaaagta ccagtattcc tctgcggtta aatttaagaa aatatctggc
     2101 gagtccggta cataacctat ttttttctta atttggtcac gatgttctgt caaaagtagg
     2161 tcatcaatat agacttggcc ataagttgca tcaataatag atgttagaat actaattgtc
     2221 gttgtttttc ctgctccatt gtgacctatt aatccaaaaa tctcaccatc tttaacagag
     2281 agggttaaat cactgagagc ttctttttct ccgtatactt tcgaaacatg ttcaaactta
     2341 atcataaaaa tctcctttta tctcctaatg atttccaaat actttagaaa gcaatcccgt
     2401 tgttacctta ttttgctcat atctctcaga caatttttta gcagattttt tcaattcctt
     2461 aactgtagct aatgactcga aagatttttc tatctcatta ttttccaatt gctgagttaa
     2521 ctcataagca acacgaggta catcgctagt ttttatcaaa ccattcgatg ctgataatag
     2581 tattgatgat agcactgcat cttcacggac agatatatca ttgtaagtat gctgtatctg
     2641 ccgataaaaa tcacgacact ccttcttcat aaacaaacct ttctaaaaag ataattaata
     2701 atagttgtat tattttatat atacaatagt tttatcaatt atactgcgct atggaatgtt
     2761 tgtcaaacgt tttcaaaaaa gagtgctttc catcacttat cagtctatca aaactattct
     2821 caaatccatc ctatatctta caaaaaagaa gttgaggtat ctttcccaac ttcttcatgc
     2881 tcttaaagtt ctttatatag cgatacagca ttctcaactg taaagccata ctcttcgaaa
     2941 attctgttac ctggtgctga tgctccccaa gtatcaatag taagtgttag cccatttaaa
     3001 ccaacatatt taccccaacc gaagcttgat cctgcctcaa tagcaagacg ttttgtaaca
     3061 gctgatggaa gcacttgctc ttgatatgaa gcctcttgtt catcaaaaat gttttgtgct
     3121 ggcatagata cgacgcgaac atggatgccc tctgactcaa gtttgtcttg agtatctaaa
     3181 gctaatttaa cttctgaacc tgttgcaatg atgataccat caagttcacc tttagcttct
     3241 gataaaatat aagcaccttt attaacccct tcttgggcta attctgatgt tccttcaaga
     3301 actggtaagt tttgacgtgt taaaacaagc atcgttggtc gatctgtctc tgatacagct
     3361 cgttgccatg cagcgtttgt ttcattaccg tctgcgggac ggataacgtt caaattaggc
     3421 attgagcgca ctgatgctaa ttgttctatt ggctcatgag ttggcccatc ttctccaaca
     3481 gcaatagaat catgtgtcat aacatagaca gttggcaagt tttgaagtgc agccatacgt
     3541 actgctggta acaagtagtt tgagaagaca aagaacgtac ccccataaac acgagtccca
     3601 ccgtgaagcg caattccatt cattgcagca gccattgcaa attcacgtac accaaaccaa
     3661 atattacgcc cagcgtagtt acttgcttgg aaatctgttt ctgctgctac cattgtgtta
     3721 ttagaagctg agaggtctgc tgaccctccc cagaggttcg gtaattgagt cgataattgt
     3781 tgaatagctt gttggcttga aacacgacta gccactgatg aacctaggtc atgagctttc
     3841 agttcaactg tctcagcttg gccagcaaaa gcttctttgt attcagttgc taattctgga
     3901 tactcaactt catatttagc aaaaagatca ttccatgcct cttctgcctt cgcaccacgc
     3961 gcttgaagat ctgaggcaaa tcgatctgca acttcagcag gaacagtaaa gtctggatat
     4021 tcccatccat aagccttctt ggcaaaagta ataccttctg caccaagtgg cgctccatga
     4081 actgaagatg ttccctgttt ctctgcgcca aatccaataa tagttttaac ttcaataatg
     4141 gttggtttat cagtttcagc tttagcagct tcaattgctg cagcaatcgc ttctaaatca
     4201 ttaccatctt taactaagat atgttgccaa ccatatgatt caaaacgtcc tttaacatct
     4261 tctgtaaatg attgagatgt tggaccatca agtgagatat catttgaatc ataaagaagg
     4321 accaatttac caagttttaa gtgtccagca agactggcag cttcttggct aacaccctcc
     4381 atcaaacaac catcaccatg aagtgtataa gtgtaatgat caactaaatc aaagcctggt
     4441 ttattaaatt tagcagccaa atgagcttca gccattgcca taccaacagc attagcaata
     4501 ccttgcccta aaggtccagt tgttgcttct acaccatcag tatggttaac ttctggatgt
     4561 ccaggtgttt ttgatcccca ttggcgaaat tgttttaaat catcaattga taaatcatag
     4621 ccagctaaat gaagtaggct ataaagaaga gctgaaccat gcccagctga tagcacaaaa
     4681 cggtcacggt ttgtccaatt gcgacttgtt tttgggttta catttaagaa tttattccaa
     4741 agcacataag ccataggcgc agctcccata ggaagacctg ggtgcccaga atttgctgct
     4801 tggatagcat caatagaaag cgtgcggact gtattaaccg ctaattgatc aatggtgtca
     4861 aatgtcatga taaaaccttt ctgtttttaa caactaaatt taaaattgtt cgtctttgat
     4921 attataacat aaaatacgta aacgttttaa caatttctgg agggattaaa accattcaat
     4981 taagtaatgg gattaattgt cataaataag tgaatctatt tcctgtagtt ctcgctctga
     5041 agcaccttta ttaaggcaat aggttagttt ggctttataa ggtcttgagt gactctgata
     5101 aataaccata tcaaatccct ttccttcgtc ataaggttcg acttgaatag aattattacc
     5161 agagaaatgt tgtcgaatgc gatattccaa caatgactgt tccacagcat tacctgtcat
     5221 atctagacca atcctaaagg gcattttttg actatttttc gagaaaataa gcaattccaa
     5281 caaatgtaaa gtcagcaaag aacaaccatc ttctgttaaa ggtctaattc gattaacatt
     5341 tgcaataatc ttctttgaaa tatgatgcaa cttatccctc atattatgat caataagtcg
     5401 atacgtttgt tctaaatcag gttgttcaac taaaatatga cctttaaaga aataataatg
     5461 gctatataac tgaccaagtt ggtaaataac ctgatctgat gtctcgtcag ctaaaatact
     5521 ctccttttta aagtagatga ttgtttcggt tacacaatca ataatcgggc ctccaaatcc
     5581 catgatgtat tccattgtat tgattggtaa aatattttgt gaatgtagaa aagcaaaagt
     5641 tgccagaact tctccttccc tatactcaag agcaaaacga ctcatataca aaactaaggc
     5701 tgcgtctaac cgtttataaa aagcattaca ttgataatgt ttcacaatag gcttgagatt
     5761 atcagatatt ttttccatag caaggagacg atgatgacaa atactaaacc aaagtcccag
     5821 attttgatga gcttctcgag ttaaagtatg ttgagccaag cgctctatta atagaattaa
     5881 tgagtctaag tctaatttct ttatcatatt ttggcacttg tcataagacc atagttgctt
     5941 gaataattca taataaaaat aacgccattg caattcatca ccaatctgtt ttccttgtga
     6001 aatagcaata tcaaattccg ctaacaattc attgagatga gcaagatgac gatttaaagt
     6061 agcctcacta accatcaatt cttgagaaag accaacgatt gtgaactgac gctgattaaa
     6121 taaagatatt aagatagtgt atttaatact atctttcatc agagctttca acacttcttg
     6181 attggataag ttataatcct cttctaaaaa taacttatcc ttataataaa cgattcgaca
     6241 attaactttt tcttgttcaa agtagctatt aagattattg atatatttaa gcaaagttgc
     6301 tcgacttaaa ccagtttcta ataaagctaa cttcagtgct attgcctgag gagagcctct
     6361 taaaaaagaa agtagccgat attgaacacg cctttctttt tccattagag cttctattcg
     6421 catagcccaa tcctcttttc ataaaggttg atagctgctt gatgcaacat ataagccatc
     6481 tcagattctc tagctgtata aatgaaatct tgaaaagcca gatcaaaatc tgtcatatca
     6541 cacgatatag cttgaacaag ctgattggca gtagccaaac aattttcctt tgagattaat
     6601 tgagccccca ataaatggcc cgttttttgg ttagcaatca attttatcct taaatcatac
     6661 tgttctttag ggaaataagt aacactaacg gtatcctcgt aaaaaccagc ttccaattca
     6721 gttaatccaa tgttagctct ataatagcca aagtgtttag atcctgtagc gcgtgttatt
     6781 tttaattttg gaacagcatg tcccgataaa tggtatgcca acatttgtgc cgaacggatt
     6841 gcttggttaa ttaagggcat ataataagca ctattaagac ctttaaaata cgaccaaatc
     6901 aaatcgccaa tagcatagat attagggtca ctcgtttgga aataatcatt tacaacaaca
     6961 ctattatcaa cacttaaatc aaccaaccca gttaaaagac gactatccgg acgaaaatta
     7021 acagctaata tgacagcatc aactgtaaag gtgtctcctg ttagcgttcg caaacaaagt
     7081 ttttcttctc gggttacagt aattttctca accttttgat tttggaaaaa atgtagtttt
     7141 gattcagcca tcgcatcaag taaaggaagg gacatatcag gatctgtatg cctgaagtct
     7201 ggtctttctt gagcttctaa caaagtgacc gccttccctg attcatgtaa actataagca
     7261 atatctaaac cgatgatacc tgccccaaca actgctacct tatgactttt atctaatacc
     7321 tgcttagcat tttgactagt tgcataagtt tttgttgtta aaacaccttg cgcatcagcc
     7381 ccatcaatat aagttgattc tgcttgggag cccatggcac aaatcaattg atcataatag
     7441 acagaagaac catctgataa cattaattcc ctatcttctt tattaatttt ctcaactttt
     7501 agaccaaata gacagcgtat attctgcaac cgttgctctt cttctgtctg ccacatggcc
     7561 tgatttaaac cactaatttc gtgacgataa taccaattta agccatttgg aacataatct
     7621 ggatgaattt ctttatcaat caaaacgata tcccaattag gatttaattg gcgtaatttc
     7681 tgcgcacaag tcataccagc aaaagaagct cctaaaatga caattacttt atttacatat
     7741 ttttctgtca taattactta ttataacaaa agaagttatt tttgaaaacg gtaacaaaaa
     7801 actgagacca taaagtcggt ctcagcatca aaaatctctt acatcatagc aaatattaag
     7861 gctgctaaaa tagctcctac cataggtcct actatcggaa tccatgaata agtccaatca
     7921 gaatcacctt tgttcttcat cggtaacaag gcatgcaata aacgaggacc aaaatcacgt
     7981 gcaggattaa tagcataacc tgttgttcca ccgagcgata gaccaatccc aaccactaac
     8041 ataccaacac tcatcgtacc aactcctgga ggcatattgt attttccgat ggcaaaaata
     8101 gtaagtacaa gaacaagtgt tcccaatact tcacttaaaa aattggaaaa attatccttc
     8161 aaagccggac cagttgcaaa agttccaagg atgtctgctc tattttctgc agcctcataa
     8221 tggggtctaa attgcaaata aacaagaagt gaacctagca ttgcaccaat aatctgtgca
     8281 atcatataaa gaatagctgt tccccaagct aaatcacctt tgatcgcaaa agctaatgac
     8341 acagctggat tcaagtgggc agggctaata ttgccagata ccaacgctgc aatagctaca
     8401 gcaagtcccc atccaaatgt gataacaatc catccagaat tatgattctt cgttttaggc
     8461 aaaacaacac ctgctacaac accattacca agtaagacta agagagctgt tcctaagaat
     8521 tctccaaaaa tatccattat tatctccttc aattatcctt caaagctgcc aagtcattgt
     8581 cttttagagt ctcatgtaat tcttcttcat agaaagtttt gtcttgatca gaccattgat
     8641 aatgtttagc catctcatca ataacaggct gaatcaaaga atctaaatta tctctcatga
     8701 aaagcatatg gttcgtacgt cttaagaaga aatccgttgg gctaagcgcc atttcataat
     8761 ccatagcata gtgaagtgac aacgtttcta ccaagctcag accttctgct tctgtaattt
     8821 ggcgtgtcaa tgcaaataat tttggtgcat ttgacccata aaggttagca atataacgtg
     8881 catcctcaat acttaaacca cttagtgtgc ccaattgagc ataagcttca atttcagaat
     8941 caacattaga tgggttaatc tctcctcctg aaacaggata ggttttagaa ttgattagtt
     9001 taaacttgcg attatattct ttatccaaaa tgttaataat agtttccatg gcaccttctg
     9061 ccatttttcg ataatcagta attttaccac cagcaagtgt taataggcca ttatcatcac
     9121 gctcaaaact tgatcctcga gaaacagctg atgggtccaa ttctttttca gaagtactag
     9181 attctacatg agaaatagct ttttcaacat cctcacgatg atttttatgt gcaatataat
     9241 ctttaacgga atcaattaat tcttcaaaac tttcatcgct tagttttcca ctattgcctc
     9301 cattatagtc agaggcacta ttccctgata gtagtggacg caaacctgcc catgaactct
     9361 caatatcatc aatagtaagc tctgcttctg ggaagcgttt gtttacaata ttaaggaggt
     9421 agtcaacatc ttctttagtt accgtaggat gttctaagtc tccatggtaa tcagtatctg
     9481 tagttccaaa ataagtctta tcttcacgtg gcaaaacaaa aatcatgcga ccatcattca
     9541 ggcctgtatc aacataaaca ggttgagaaa tatttaattt ttggcgatct acaactaagt
     9601 gaactccctt agttggacgt aattgatgaa tttgtttacc ttcattggag aagttgcgaa
     9661 cggtgtcact ccaaggtcca gttgtattaa taacaagcct agcttttata tcgataactt
     9721 ggtcagtcaa caaatcacgc gcacgtactc ctataatttg attgttgtca tcaaataaga
     9781 agtcctcagc tttgacatga ctggcaatat aagctccatc cctattagcg cgtttaatat
     9841 tttcaatcac taggcgtgca tcgttattac ggaaatctaa atagacccct ccgcctagca
     9901 aaccttcttt ttgtaaatca ggctcacgct taagaacatc ttctgcgctt aaaactttat
     9961 tggctgcggg tgtgtttgtc acacctgcta ataaatcata taaatccata gccactttaa
    10021 ggcgaaacat actaaaagta gaacccggct catcgtaaac cggtaaaagc attggatctg
    10081 gcttaggaat gtgtggtgca atttgctgca cgactgcacg ctctgatact gtatcagaaa
    10141 caacttctac atcaaattgc ttcaagtaac gaagaccacc atggaccaat tttgttgagc
    10201 gactacttgt cccttctgca aaatcttgca tttcaataag tccagtatct aatccactag
    10261 ctgctgcttg taaagcgaca ccagcgcctg tgataccccc acctatgata agtaaatcca
    10321 gagtacgatc ttgcattctt tgtaaggcta aacgtcgtgt ttctcgtgaa aattccatct
    10381 ctacaccccc ttaatcctct tgagcaaata cttgtgtcgc tttgacagct tttctccacc
    10441 ctttatacag tttttctttt cgagactcat tcattgttgc ttggaaaagt tgtcctgttg
    10501 cgttcagttc ttttagttca tccatacttt cccaatagcc aactgaaagt ccggctaaga
    10561 aagctgcacc caaggcagta gtttcaaggt tcttcgcacg agcaatatca atccctaaaa
    10621 tatccgcttg gaattgcatt aataaattat tcattgcagc tccaccatct acacgaagtt
    10681 gttggatatc aattcctgaa tcaacttgca tagtgtcaat aacgtctcgt acttgataag
    10741 caattgactg aagtgtagct ttaacaaaat cttctttact tgtcccacga gttaatccaa
    10801 atactgaccc tctagcattt gaatcccaat acggtgcacc caaacctgta aaagctggaa
    10861 ccacataaac ctcatcgtca cttgttgatg attgagcaag gccttctgac tcacttgacg
    10921 tttcaatcat tctaagacca tcacgtaacc attgaatagc gcttcctgca atgaagattg
    10981 atccttcaag tgcataatga accttaccat taatgccgta accaatcgtt gttaatagat
    11041 tattttgaga cagttgcatc tcttcgcccg tattcatgat aatgaaggaa cctgtaccat
    11101 aagtgttttt taccatacca ggttcaaagg ccaactgtcc aaataatgct gcctgttggt
    11161 caccagccat accagaaatt ggcacctctc caccgtaaaa atggaatgga gtagttttcc
    11221 cataaacttc agaattagat tttacttctg gtaacattgc tttaggaata tttagtaact
    11281 ctaaaatttc atcatcccat ttaagttctt taatattata aagcatcgta cgagcagcat
    11341 ttgaatagtc tgtaacatgg acgagaccat cagttaactt ccaaactaac caagtatcaa
    11401 ttgttccaaa aagcaactct cctttttcag cacgttcctg tgctccagga acgtggtcta
    11461 aaatccaacg aacttttgtt gctgagaagt aagcatcaat gacaagacca gttttttcat
    11521 gaatcatgtt agtatgacct tcttgcttca attgatcagc aatcggggct gtttgtcgcg
    11581 attgccatac aatagcatta taaattggaa gacctgtttt tttatcccaa acaacagttg
    11641 tttcacgttg attggtaatc ccaatagcct ctatctgtcc aggtttgatg cttgattcaa
    11701 taaaagctcc agcaatgaca gattgaacag aattccaaat ctgattagca ttatgctcta
    11761 cccagcctgc ttgaggaaaa atttggggaa attctttttg gctactcgca attttttcac
    11821 cttttttatt aaaaataatt gctcgagagc ttgttgttcc ctggtcaatc gccataatgt
    11881 atttttcttc tgaagacata aacttcctcc ttaaaagtaa gcgttttctt tttgtaattc
    11941 tattatacct aaatcagtct atcctgcact atcaaaaatt taatatgctc ctaaaatttt
    12001 tgataagata tcatcaataa caccaaatta gccaaataat agctaaatca ataatcattc
    12061 cgatatacca aactagtctg aaataccatt tagtcgtttt atgacgaaaa acttctccac
    12121 ctaggaatgc accaaatccc cctaataata aactctctat catcaatgtt ttctcactaa
    12181 tacgccatgc ccctctaatt gcacgccttt tatcaatagc ataggttgca aatactaaaa
    12241 tattccataa cagtaatgct aatagtaatt caatcattat tttttccttc ctaataaaaa
    12301 aggcgagaca aagattatct tttgccccac ccaagtgttg actttaatct aaaattgtct
    12361 caccattaag aatttggatc atccaaacta cgaccatgta gtcctttttc tctctggact
    12421 tgacgaagtt tttccggggt aacatcattg ccctcatcat ccactaattt aattccttca
    12481 acgtggtgac ggactgatcg gcggaaacct tcaatatact cttcacggag ctttgcttgt
    12541 tcaaccttct cttctccggt caatccaact gtttttttct tttttgaaag ctcgttaata
    12601 cgagcaattt ttttaggatc cataaaatgc ctccttattt tgtgtttaat aagttaaaag
    12661 cagcgactgt tcttgccttt gcaacaaggc tattgagtaa agccagacga ttttgtttca
    12721 ttttttcatc atctgtcatt accattgtat tatcaaagaa atcattaata attggactaa
    12781 gtgcgaaaag cttatctaag ttgtcatgca tatcctcagt cagttcaagt gacaaaatag
    12841 cttgatatag cgccttctct tgattatttt caaacaatga agagtccacc aatacagaat
    12901 gagtaacttt ttcagctaaa ttaaatgcac gtgacaaact ttcaacggaa actttatatt
    12961 gagcatcctt gctcttttgc accaatgctt gactagcttc tagaataagg ctaacaatat
    13021 aagtgtctga ttctaaaacg gcttctcgaa tatctttagg gaccttagag ccaatcatct
    13081 tttcaatacg agctgaaata aaggccatga cctctttttg attagcataa tctaaactag
    13141 caaacgatag accataaagg ttagttacta attcatcaag tgggatatcc caaccaaaag
    13201 cttccaaaat acggacaatc ccctgagtag ctcgacgaag agcatatgga tcatttgaac
    13261 cacttggaat caaaccaact gagaagaatg aaagtaaggt gtcaaattta tcagctaaag
    13321 ctagaattgc accaactctg gtttctggaa gctctccatc tgcagatgtc ggcatataat
    13381 gttcacgtat cgctgctgct actgcgggct gttcccctgc aagtaaggca tatttctcac
    13441 ccataatacc ttgtaattca tcaaactcat caaccattcc agttaagagg tcaaatttat
    13501 aaatacttgc agctcgaagc acagctaatt tttcttcatc agaaagatct gccttctcag
    13561 ccaaatattg tgaaataact tttacacgat ccatatgttc ataaagtgaa ccaatttttt
    13621 catggaaagt tacttgtttt aatttctcaa ctaaatctgc aatattaagt ttctgatctt
    13681 cttgccagaa gaattcacca tcttcaagac gagccactaa aactttttca ttacctttta
    13741 taacattttc gatatgctca gcatttccat tacgcacaga aataaagttt ggtaacaatt
    13801 ttccatccct gtcgcgaaca acaaaatagc gttgatgatt tttcattgaa gtgactaaaa
    13861 cttcttcagg tacatcaagg tatttctcgt caaaagaacc caagaatgct gtcggatact
    13921 caactaaatt aagcacttca tttagtaaat cttcgtcaat ttcaactgaa atatttttct
    13981 tttcttcgat agcatgaatt tgattaacaa tcatttgctg gcgttctttt gcatcagcaa
    14041 taacaaattg ttgtcgcaag tcatcttcat acgaactggc agatgaaatt tcagtgtcag
    14101 aacccaagaa acggtgtcct cgactaatac gccctgaatg aatatcaagg aagtctagct
    14161 ctaaggcttg atcatctaat aataccacta aagtgtgaac tggacgaata tattcaaaac
    14221 tattatttgc ccaatgcatg ttcactggga aagtcaattc tgttaatacc tctgtcacac
    14281 tcgctaaaac atcaatcgca ctttttccag tttcatgctt agtgacataa acatattctt
    14341 ctcccttaac ttcacgaaat tcgatatcat caacacttaa accttttccg cgaacaaaac
    14401 cttgggctgc tttagaaaaa ttcccttctg catccaaagc aattttctta gaaggaccct
    14461 tgaaatcttc tgttaaatct gtctgctggt ctgccaatcc tttcacacga acagccaaac
    14521 gacgtggagt tgaaaaagtt tgaacagttt caaatgataa acgatgatcc tctaaaaact
    14581 tgaccatttt ttggcccaat tgcttctcac tcggtgtcac aacataagct ggcaactctt
    14641 caaggccaag ctccaataat aaatctttag tcatttccta ctccttactg taaaacgctt
    14701 caaatgcttc tcttaaatcc tgattcatct tatcaagaac aacttgccta tctaaaatat
    14761 cggtaccttc tgcacgaaca acacagaaat catcaaaagc cataaaatct ttaaagatag
    14821 cttttaaata atgaacagga aattcatatg gactatactg tcctttagaa taaatagaac
    14881 ccgcagattc taacataagc agacgatagt catctgtcat taatccgact gaaccatttt
    14941 cagtgtactt gaaagtctca cctgcaataa aaatattatc aatataagtt tttgctctag
    15001 cagtcacatt gtaattgtgc attggagaaa caaagacaat acggtgatgt gacttaaact
    15061 gttcaagtaa atctttagag attttagctt gaacaatctc atcggctgtt aattccaaac
    15121 cctttctttg tttagaccaa atcgaaagta gtacttcttt cgtaatttct ggcaaaacac
    15181 aatcatataa atttaaaata gataactgat ggttaggaaa atgctcatta taaagttcaa
    15241 taaacttttc ttgtagaata gttgtaaatg agtacggatt cgaaaaatca ggatgactat
    15301 taacaatgag tgtgttcatt agtcttcctc agcaagtaat ttgatacgag tttcctcatc
    15361 caaaagtggg aaaccaagtt ttttacgttc ggcaacaaag gttttagcaa caacacgtgc
    15421 taaattccgg atacgtgcaa tatagcctgc acgctcagta acagatactg ctccacgtgc
    15481 atctaataaa ttaaaggtgt gtgaacattt aaggacgtag tcataagctg gatgaacaag
    15541 tccttcttct aaagcacgtt ttgcttctct ttcaaatttc tcaaagtttt caagtaacat
    15601 tacttggtct gatatctcaa atgaatattt tgaatgctca tactctggct gagtaaagat
    15661 ttcaccatat ttaacacctg gtgcccactc aatatcataa acagaatcta cctcttgaat
    15721 ataagaggct aaacgctcta gtccataagt aacttcagaa gttacaggac ctgtttgaag
    15781 tccacctact tgttggaaat aagtaaattg tgttatttcc attccatcaa gccaaacttc
    15841 ccaacctaat ccagctgaac ctgtagatgg attttcccag ttatcttcaa caaaacggat
    15901 atcatgctca agtggattga tacctagtaa ttccaaagac ttcaaataaa gttcttgaat
    15961 atttgacggc gaaggcttca ttaccacttg aaattgatga tgttggtaaa gacggtttgg
    16021 attttcgcca tagcggccgt cagctggacg acgtgatggt tcaacgtatg cagcattcca
    16081 aggctcaggg cctatagcac gtaagaaagt ataagggctc attgtccctg ctcctttctc
    16141 attatcataa gcttgcatta acatacagcc ttggtcattc caaaactgtt gtaaagttag
    16201 aataatttct tgaaaagtta atttcttaga catcattttc cttctttcac actttattac
    16261 agctgttggg aaggtgacaa caattgactt aaaacggaca aatgataaaa accacgtcct
    16321 acaccaccta tgcccaacag cacaggggcg tcaaagacgc ggttccaccc taatttatta
    16381 cttcattttt gattgtactt ttactcacta aaaagtgcca atattcatcc tattctaccc
    16441 aactttcact accttgagct cgctaaaaaa acagctgatg aacacctctt cttactgaga
    16501 cagtataaca aaaaatgact tttatgtcta ctatttatct tattcttaat ataccctaga
    16561 ctttttgatt ccatttggaa taatgatcgt ttttaataat aagtttt
//
LOCUS       DATRSD010000051        16533 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRSD010000051 DATRSD010000000
VERSION     DATRSD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16533
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.052"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            76..1821
                     /locus_tag="VBD85_001317"
     CDS             76..1821
                     /locus_tag="VBD85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133873.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGLVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEVNQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKKATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIIENLKTNRQGKSTIISAHRLSAVVHADLILVMQDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            1811..3550
                     /locus_tag="VBD85_001318"
     CDS             1811..3550
                     /locus_tag="VBD85_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133874.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMSLDSLFLRPVMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            3678..4244
                     /locus_tag="VBD85_001319"
     CDS             3678..4244
                     /locus_tag="VBD85_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO7133875.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(4311..4850)
                     /locus_tag="VBD85_001320"
     CDS             complement(4311..4850)
                     /locus_tag="VBD85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7133876.1"
                     /translation="MNTRTTTYIALMVALLIVLGFIPGIPLGFIPVPIVLQNLGVMLA
                     GALLGSRKGFLAVAIFLLLVAIGAPFLPGGRSGLVTLFGPTAGYLLTYPFAAFFIGLG
                     LEKVKTTKLWVQFLIIWIFGVLLIDICGSIVLSFQTSLPLTKSLFSNLIFIPGDTLKA
                     SICLIIYRKFANRLTHLYN"
     gene            complement(4851..5843)
                     /gene="bioB"
                     /locus_tag="VBD85_001321"
     CDS             complement(4851..5843)
                     /gene="bioB"
                     /locus_tag="VBD85_001321"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO7133877.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEDGRNQHTDIEFLEKLQLNHTKKELI"
     gene            5967..6482
                     /locus_tag="VBD85_001322"
     CDS             5967..6482
                     /locus_tag="VBD85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133878.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKVSLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRKGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            6472..7587
                     /locus_tag="VBD85_001323"
     CDS             6472..7587
                     /locus_tag="VBD85_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO7133879.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIEP
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     DINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREILDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKETFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVQTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            7580..8809
                     /locus_tag="VBD85_001324"
     CDS             7580..8809
                     /locus_tag="VBD85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7133880.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLEVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGGDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMEQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            8922..9554
                     /gene="nth"
                     /locus_tag="VBD85_001325"
     CDS             8922..9554
                     /gene="nth"
                     /locus_tag="VBD85_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO7133881.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(9555..9950)
                     /locus_tag="VBD85_001326"
     CDS             complement(9555..9950)
                     /locus_tag="VBD85_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO7133882.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQEYPIFLWILFTISTIVLTP
                     ITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCL
                     VMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
     gene            10106..10315
                     /locus_tag="VBD85_001327"
     CDS             10106..10315
                     /locus_tag="VBD85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO7133883.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            10312..11280
                     /locus_tag="VBD85_001328"
     CDS             10312..11280
                     /locus_tag="VBD85_001328"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO7133884.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFMCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            11292..11672
                     /locus_tag="VBD85_001329"
     CDS             11292..11672
                     /locus_tag="VBD85_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7133885.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            11904..13745
                     /gene="typA"
                     /locus_tag="VBD85_001330"
     CDS             11904..13745
                     /gene="typA"
                     /locus_tag="VBD85_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO7133886.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            13790..14035
                     /locus_tag="VBD85_001331"
     CDS             13790..14035
                     /locus_tag="VBD85_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO7133887.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKEILNLSEKKK"
     gene            14165..15520
                     /locus_tag="VBD85_001332"
     CDS             14165..15520
                     /locus_tag="VBD85_001332"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO7133888.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMLVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            15523..>16533
                     /locus_tag="VBD85_001333"
     CDS             15523..>16533
                     /locus_tag="VBD85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO7133889.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSE"
BASE COUNT         5385 a         2614 c         3312 g         5222 t
ORIGIN      
        1 acttttcatg tcttctgatt gatttggtct tctgacttaa ttatgatgct tttcaatctt
       61 tgagaaagga aaaatatgtc tattataaaa aatttgtggt ggttttttaa ggaagaaaag
      121 aagcgctatc ttattgggat tttgtcatta agtttggttg cggtgttaaa tcttatccct
      181 cctaaaatta tgggattagt tattgatgct attacaactg gaaaattaac aagaccacaa
      241 ttactatgga atttattagg tttggttttg tcagctttag ctatgtatgg gctgcgttat
      301 atttggcgta tgtatatttt agggacttct tacaaattgg gccaagttgt cagataccgt
      361 ttatttgaac attttacaaa aatgtctcct tctttttatc agaaatatcg tacaggtgat
      421 ttaatggcgc acgcgaccaa cgacatcaat tctctaacac gtcttgcagg aggaggagtt
      481 atgtcagcag tggatgcctc tatcacagca ttagtaacgc ttatcaccat gttctttact
      541 atttcgtggc aaatgacatt aattgcggtt atccctttgc ccttaatggc cttagcaact
      601 agtaaattgg ggcgaaaaac ccatgaaacc ttcaaagaat ctcaggcagc cttttcagaa
      661 ttaaataata aagtgcaaga aagtgtctct ggcgtcaaag tgactaaatc atttggttat
      721 caagaacaag aaattgcttc ttttcaagag gttaatcaga tgactttcgt gaagaacatg
      781 cggaccatga cttatgatgt catgtttgat cctttagttc ttctttttat aggtgcctcc
      841 tacgtattaa cattggctat gggagctttt atgatttcaa aaggtcaagt tactgttggt
      901 gacttggtaa catttgtgac gtatttagat atgttggtat ggcccttgat ggcgattggt
      961 ttcttgttca atatggtaca gcgtggtagt gtttcttata accgtattaa tagtctactt
     1021 gagcaagaat cggatataac tgatccttta aatcctatca aacctgttgt caatggaaca
     1081 ttaagatatg atattgattt ctttagatac gacaatgaag aaaccttagc cgatattcat
     1141 ttcaccttag aaaaaggtca aaccttaggt ttggtaggtc aaacgggatc agggaagaca
     1201 agtcttatta agttattgct acgtgaacat gatgtgactc aggggaaaat tactttaaat
     1261 aaacatgata tacgtgatta tcgattgtct gagttacgtc aactaatcgg ttatgttcct
     1321 caagatcagt ttttatttgc taccagtatt ttagaaaatg ttcgctttgg aaatccaact
     1381 ctatctatca atgctgttaa aaaagcaact aaattggctc atgtttacga tgacattgaa
     1441 cagatgccag caggatttga gactctaatt ggagaaaaag gagtctcatt atctggtgga
     1501 caaaaacaaa ggattgcgat gagtcgtgcc atgattttag atccagatat tcttattttg
     1561 gatgattctc tatcagcagt ggacgctaaa acggaacatg ctatcattga gaaccttaaa
     1621 acgaatcgtc aagggaaatc gactattatt tcagcacatc gtttatcagc tgttgtgcac
     1681 gcagacctta tcttagttat gcaagacggc agagtcattg agcgaggtca acatcaagag
     1741 ttgctaaata aaggtggttg gtatgctgaa acgtatgcct cacagcaatt agaaatggag
     1801 gaagcatttg atgaagtcta atcaatggca agtctttaag agattaatct cctatttacg
     1861 cccttataaa tggtttacag tattagctct atctctctta ttgttgacga ctgttgttaa
     1921 aaatattatt cctttaattg cttcacattt tattgatcac tatctgacaa atgttaatca
     1981 aacagcagtt cttattttag tgggatatta ttcaatgtat gtcttgcaga ccttaattca
     2041 atattttggg aatctctttt ttgcgcgtgt ttcttatagt attgttagag atattcgtag
     2101 agatgctttt gctaatatgg aaaggctagg catgtcttat tttgatagga caccggcagg
     2161 atctattgtg tcacgtatta ctaatgatac tgaagcaata tctgatatgt tttcgggtat
     2221 tttatcaagt tttatctcgg cgatatttat ttttacagtt actctgtaca ctatgttgat
     2281 gctagacatt aaactaacag gactcgtcgc tcttttgtta cctgttatct ttatattagt
     2341 gaatgtctat cggaaaaaat cagtcactgt cattgctaaa acgagaagtt tacttagtga
     2401 tatcaacagt aaattatcag aaagtattga aggaattcgc attgtacagg cttttggtca
     2461 agaagagcgc ttgaagactg aatttgagga aattaacaaa gagcatgttg tgtatgccaa
     2521 tcgttctatg tctcttgata gtctcttctt aagaccggtg atgtctcttt taaaactcct
     2581 agcatatgct gttcttatgt cttattttgg atttacagga gttaaaggag gtcttacggc
     2641 aggattaatg tatgctttta ttcagtacgt taatcgtctc tttgaccctt taattgaagt
     2701 aacgcaaaat ttttcaacct tacaaacatc aatggtatca gcagggcgtg tgtttgatct
     2761 gattgatgaa acaggttttg aaccaagcca aaaaaataca gaagcttttg tgagagaagg
     2821 aaatatcgaa tttaaaaatg tatcattctc atatgatgga aaaaaacaaa tccttgataa
     2881 tgtttctttt agtgttaaaa aaggtgaaac tattgctttt gtaggagcga caggttctgg
     2941 aaaatcatct attattaatg tctttatgag gttttacgaa tttcaatctg gacaagtttt
     3001 attagatggg aaggatatta gggattactc acaagaacaa ttgcgaaaga atattggact
     3061 cgtcttacaa gacccctttt tatatcatgg cactattaaa tctaatatca aaatgtacca
     3121 agacatcacc gatcaagagg tacaggacgc agcagagttt gttgatgctg atcaatttat
     3181 tcagaagttg ccagataagt acgattcagc tgtttcagaa cgtgggtcaa gtttttcaac
     3241 tggacaacgc caactattag cgtttgccag aactgtggct agtaaaccta aaattttaat
     3301 cttagatgag gcgacagcaa atattgattc ggaaacagag cagattgtac aagattcgtt
     3361 agcaaaaatg cgtcaaggtc gaacaacaat tgccattgct caccgcttat caaccattca
     3421 agatgctaat tgtatctatg ttcttgatag aggaaagata attgaaagtg ggaatcacga
     3481 gtctttatta gaccaaaaag ggacttatta tcgtatgtac caattacaag ccggcatgat
     3541 ggaggtctag aaaatttagg ctattcaaaa ggcataatct gtgaggctta gaggttggaa
     3601 tagttattcc tctaagcctt ttttatattt tacaattagt gtataatgag gtcaaagtac
     3661 aaggaaaggt ctataaaatg ttactattgg tagataatta cgattctttt acctataatt
     3721 taaagcaata tttaagtgtc tataaagaaa tattcgtcat aaaaaatgat gatcctaacc
     3781 tttttttatt agctgagagc gcagaagcta tcgtcttatc acctggacca ggacacccaa
     3841 aggatgctgg gaaaatatta gaattaatca atcagtttat aggacagaaa cctattctag
     3901 gtatctgttt ggggcatcaa gcgcttgctg agtgcttggg tgggcgatta aatttagcaa
     3961 atcacgttat gcatggtaaa caaagttggg taacaataaa cgaccatact agtttgttta
     4021 agggaattga tagtccaacc caagttatgc gttatcactc attggtagtg actgatttac
     4081 cagagaatat tgctattatt gcacgatcaa atgaggataa tgaaatcatg gcattccatt
     4141 gccccagctt aaaggtatat gcgatgcagt ttcatcctga gtcaatcggg agtattgatg
     4201 gaatgaaaat gattgaaaac tttttgacgt taattaatga ctaaaatgtg ttagtgtagg
     4261 ataatgactc tctataaata aaagacagta ccataggtac tgtcttttat ttagttgtat
     4321 agatgagtta atctatttgc aaatttacga taaataatca agcaaatgga tgcttttaag
     4381 gtatccccag gaataaaaat aaggttagaa aatagtgatt tagttaaggg gagagaagtt
     4441 tgaaaggaaa gaacgatgct accacaaata tcaatcaata gtacgccaaa tatccaaata
     4501 ataaggaatt gaacccagag ttttgttgtt ttcactttct caagccctag gccgataaaa
     4561 aaagctgcaa atggataagt cagcagatag cctgctgttg gcccaaacag cgttacgaga
     4621 cctgaccgcc ctcctggtaa aaatggtgca ccaatagcaa ctaagagtaa gaaaatagcc
     4681 acagctagga aacctttcct actaccaagc agagcacctg ctagcataac accaaggttt
     4741 tgtaagacaa ttgggactgg aatgaaacct aatgggatcc ctgggataaa acctaaaaca
     4801 atgagtagtg caaccatgag tgcaatatag gtagttgttc ttgtattcat ttaaattaat
     4861 tcctttttag tatgatttag ttgtaatttt tccaagaatt caatatcggt atgttgattg
     4921 cgaccgtcct ctgttaggta atttcccaaa aaagttgagt caacaagtaa ggtgactaat
     4981 gattcaaaat tctcaaaatg gacctcccgt ccagcactta atcttaattc cttgaaagga
     5041 aaaacaaaac gcaacatggc cataattttt aaacatttaa tgggagtcaa atagttatat
     5101 tttccaagag gtgttccttc aacagcaagt aaaaaattga cagggataga ataggggttc
     5161 agctctttta gtctgaaagc taatgtgatg agcccctcat cgctctctcc cataccacaa
     5221 atgaatccag aacaaacatc aatgtcttca ttgtgaattc tttctaaagt atcacaacga
     5281 tctttgaagc tatgcgtcgt tgcaatgttg gggtagtttt cttcgggggt gttgagatta
     5341 tgattgatac ggtcaattcc tgcttgtttt agctgatgaa gttgttctct atccaaaaaa
     5401 ccagcggtaa ggcatatttc tagagggagg ctttttttga tttcagggat gacttgacat
     5461 aactgctcaa tttctctctt gctaggctta gttccactca tcgcaataca aaagacgcta
     5521 gcgccatttt gatgagcgac aatagcctgt tttagaatga cattttgagg aagcagacca
     5581 aatttatcaa tgtcagcagt gctttcttta gattgagaac agtaaccaca attttctgca
     5641 caaagtcctt gcttagcact taataaaacg ttaaggcgaa tattatttcg gctaacttga
     5701 tttttaaggt ataaggcagc agcgtagatt tcccaccagt tctcatcgct atttagaatt
     5761 tccagtgcct gttcataaga aatgctagtc tttccagata atatttcgtc agccaaatgg
     5821 atataatttg tttgaaatga catgatagcc tcctttaacg aaattaataa tagcataaaa
     5881 aaactatatt gtaaacttaa tctatattat aagttaacaa tataaaaatt atgatatact
     5941 ggtcttatat tttgacggga gataagatga aaaaactatt taaaaaagcg atagctatgt
     6001 gccgagattt gccgcttaat tttgtaattg ttgaacttag tttgtatcaa agagaggatg
     6061 gctcatctga taatattaaa tggatttcta agagaatcct tgaggtaacg gtaactgatc
     6121 agcaatggat ttaccatttg aggatgctta atttaccaaa acttaagcca atgtcactag
     6181 catccagtga ctgtcctgca agggaagaga aggttagtct tatttcaata gaagaatgtc
     6241 aaaaattcac tagcttagta cgagataaaa atccttttca ccaaggggaa gcagctattg
     6301 tgcctgcggc tctattattg gaaaaaataa tggaagttag aaaaggaaaa ataaaggaga
     6361 ttcatttgac ttttttatct ccagtaaggg tgaatcaaga attctcagtg caagtagtgg
     6421 ataacacagt gattattttt cagcatcaaa tattatgtgt gagggggaca tatgaagaat
     6481 agagatgttt atattggttt tgggctacga acacctatcg gtataaaagg gaagcaattt
     6541 aaacattacc gtccagaact tttaggagca cacctcttaa atcaaataaa aaaaatagaa
     6601 ccagaatcta acattgatag tattatttgt gggaacacag ttggtactgg gggcaatatt
     6661 ggtcgtttga tgactctttt ttctgattat gaatcctata ttccagtaca aacgattgat
     6721 atgcagtgtg cttcatcaag ttctgccttg ttttttggtt atctaaaaat cagtgctgat
     6781 attaatgaaa aagttcttgt tggggggatt gaaagtagtt ctcttcaacc tatgagacgt
     6841 tacgctaaag aagataatcg taacggagaa tataccgttg ctcagttttc tcctgactct
     6901 tacgctgaaa ctgtaatgtt agaaggggcc cagagagtct gtcaaaaata tggttttaga
     6961 agagaaatat tagataaatt ggcattcttg agccataaac gcgccttaac agctaaacaa
     7021 ggtggctatt tagaagaggt aatcttacca atggaaggga tgcgagatca aggcgttaga
     7081 aaactaaaag aaacattttt tcaaaaatta ccaagattga tggaaaattc acctttgctc
     7141 actattggaa atgtttgttt aatgcacgat gctgctgcat ttctaacgct tcagagtcag
     7201 aaaacagaat tccggattgt tcacattgta gaagtagcag gagatcccaa gcttagtcca
     7261 gaattggttc aaacggctac ggaaaaacta ttaacagaaa ctcatactaa aatatcggat
     7321 tatgatgcaa ttgaatggaa tgaaccattt gcagcgattg atgctttatt taatcattat
     7381 tatcctgaag agagagaaaa attcaatatt tttggagggg cattagctta cggacaccct
     7441 tatgcctgct caggaattat taatatcctt catcttatgc aggcattaaa atataaaaat
     7501 aaacctatgg gtctaactgc cattgcaggg gcaggaggag tcggaatggc catatcaata
     7561 gagtacttag gagttaagaa tgcttgaatc attgaaaaca atagttaaaa ccaatagcga
     7621 taaaaagtta tttgatggtg atttagaggt cagttatgga gaattttata acttagtcag
     7681 acaagatatg gcgtcacaag acaataggaa acatgtgatt agcacacact ctctgcttaa
     7741 ccaattagta agatttgttt caaagttatg tcaaaaagct cttcctatta tttgtaaacc
     7801 taaccttact cataatgaaa taagtcgatt agaaaaagaa gtgcagtatg ctcctcaact
     7861 agctgatttt ggtgttcttt cttctgggac tacagctgat gctaaactat tgtggaggag
     7921 cttcgcttcg tggtctgatt tttttagcat tcagaatgct tatttttcgg tcacttccaa
     7981 tagcagactg tttattcaag gtgatttttc atttactggt aacttaaatt tagcgctatc
     8041 ccttttatta ctggggggaa cgcttgttgt cacacagaaa aactctgtaa aatactggca
     8101 gactttatgg gaaaaaactg gtgtaacgca tttatatctg ctccctagtt atctcaagtt
     8161 agtggaacaa tatagcaagg agactgcgtt agacaataaa actattatta cctcttcgca
     8221 atatgtctct gactcattac tggaaggttt atatataaag catcctaaag tgtcagtgaa
     8281 aatcttctat ggtgctagtg agctcaatta tgtgtcatgg tatgatggtc gtgatatccg
     8341 agataagcca cagtatgttg gagaaatcgt tccaaatgta gctgttagga ttaaagaggg
     8401 aagaattttt gtaaaaacac cgtatagtat ctgtggcctt tcaagtgaat attgtgctgg
     8461 tgattatggt gaactgatag atggcaaatt atatctattt ggccgtggtg gtgattggtg
     8521 caatcaatcg ggcattaagc tctatttacc tagattaatc gaaaaaataa aaacatgccc
     8581 ttatatcaag gatgcagtag catttacaaa agagagtcaa agtcatggac aagagtcaca
     8641 ttgctgtatt gttttaattg aaaatcagat ggaacaagag tgtttgaagt ggttatcgga
     8701 acattttgaa aagaagtatg gttttaagca ttatcatatt gtatctaaaa ttcctttgat
     8761 gccatctgga aaaattgatt accaacaact caaaagacaa ttagcatgag cttttactaa
     8821 aaaaataaaa tagggttaat ggattgcaaa cctcagttct gatattcttt attttgaaag
     8881 atatcgtgat ataatgataa aaaagaaaga taaggaataa aatgttatca aaagcaaaat
     8941 cacgatatat tatccgtgag attattaagt tatttccaga tgctaaacct agcttagact
     9001 ttactaatgt ctttgagctt ttggtagctg taatgctatc agctcaaaca actgatgcag
     9061 ctgttaataa agtcaccccc gccctttttg aacgctttcc aaatcctcta gttttggcac
     9121 aagctgatcc caaagagatt gaaccctata tttctaaaat agggctttac cgtaataaag
     9181 ctcgattctt aaatcaatgc gctaaacaat tgatagagca ttttgatgga aaagtccccc
     9241 aaactcgtca agagctagaa agtttgtctg gcgttggaag gaaaacggct aatgtggtca
     9301 tgagtgttgg atttggtatt ccggcttttg ccgtagatac tcatgtcact cggatttgta
     9361 aacaccacca gatttgcaaa cagtcggctt ctccactgga aattgagaaa cgagtcatgg
     9421 aggttttacc accagaagag tggctagctg ctcatcaatc catgatttat tttggtcgtg
     9481 caatttgtca tcctaaaaat ccaaagtgcg accagtaccc acaactttat cactttcctg
     9541 ataatttaaa gtgattagaa taaaagagag taactggtga cgattatata agcgatactt
     9601 aaaaacggta gaaaagctat cgtttttttc atttttctca taactaaaca ataaataatt
     9661 cctaaccaag cacctatttg aataataaaa agcatttgat gaagaggtaa agataagcca
     9721 attgtggcta aataaaggaa atctccactc ccgatattaa tatccaccac ttcagctaaa
     9781 atcccaaata aacacaaaac aatagaaact tttgtaattg gtgtgagaac tatcgttgaa
     9841 atggtgaata atatccatag aaaaatagga tactcttggt attttatatc gtaataactt
     9901 aagataaaac tcatgcataa agaaataaaa tagatggtag acataatcat aaaatccttt
     9961 ttatagttta tatttattat tcgaaaaatg gtagaaaaga gattatgatt tctattatag
    10021 atagtcagcc taacgtcata atgacaatta gcaagcggtt gcggattttc ttgaaaattg
    10081 aatggttttc tgttaaaatt catatatgaa agttttattc gatgtgcaga atcttttgaa
    10141 aaaatttggt atttatgttt atattggtaa acgtctttat gatatcgaag ttatgaagat
    10201 tgaattgcaa cgtctctacg ataatggtct gataagtaga gatgattatt taaaagcaga
    10261 actgatttta agaagagagc acagactaga attggagaag gaaaataaaa aatgagtaag
    10321 aaattattgg gaattgacct cggaggaacg accattaaat ttggtatctt gacgcttgag
    10381 ggagaagtac aagaaaaatg ggcaattgag accaatactt tagaaaacgg aagacatatc
    10441 gtttctgata tcgttgaatc tctcaaacat cgtttgagcc tctatggatt aacaaaagat
    10501 gactttctcg gtatcggtat ggggtctcca ggagctgttg atagaactag taaaacagta
    10561 acaggtgctt ttaatctaaa ttgggctgat actcaagaag taggttcagt tattgaaaaa
    10621 gaagttggaa ttccattttt tattgataac gatgctaatg ttgcagcact tggtgaacgc
    10681 tgggtaggtg ctggtgccaa taatcccgac gttgttttcg taaccctcgg aacaggagta
    10741 ggtggaggtg ttatcgcaga tggtaacctc atccatggtg ttgcaggagc aggtggagaa
    10801 attgggcata tgattgttga tccagaaaat ggatttatgt gcacatgtgg taacaaaggc
    10861 tgtcttgaga cagttgcatc agcgacaggt gttgttagag tagcacgtca actcgcagaa
    10921 cagtatgaag gttcgtctgc cattaaagca gcgattgaca acggtgatac tgttacaagt
    10981 aaagatattt ttatagcagc agaagatggg gataaatttg ctaattctgt tgttgaacgt
    11041 gtatcacgtt accttggact ggcagcagct aatatttcaa atattttaaa ccctgattct
    11101 gtggttattg gtggcggtgt ctcagcagca ggtgaatttt tacgtagtcg cgttgagaaa
    11161 tactttgtca catttgcttt cccacaagtt aaaaagtcaa ctaaaattaa gattgctgaa
    11221 ctaggtaatg atgctggtat tattggtgca gcaagcttag ccaatcaaca agcaagttaa
    11281 ggagataaga tatggatatg tctgttattt taattatcgt tattcttctg gcttttgttg
    11341 cttgggcatc ttggaattat tggagggttc gtagggcagc taaattttta gataacgagt
    11401 cttttcaaaa agaaatgtca cgaggacagt taattgatat ccgagaagct ggcgcttttc
    11461 atagaaaaca tattctcgga gcacgtaaca ttccagcaag ccaatttaaa gttgccttat
    11521 cggctttacg caaagataaa cctgtcctct tatatgacgc aagccgtggt caatctattc
    11581 caagaattgt tttgttactc agaaaagaag gttttaatca actttacgta ttaaaggacg
    11641 gtttcaatta ctggacgggt agagttaagt aatatactaa ggagagagtt actattaata
    11701 actctctcct ctttttgtag aaatgaatca aattgaaaaa agtagaaaat aaatgacata
    11761 aataatgaaa tgatgaaaag ttttcttatc acaaacaggc agttgatatg aaaacattta
    11821 cacttgtgta aattctgttt tttaagaaaa attgtgttat aattcataag ttaacagaat
    11881 tacattataa aatagaggaa aacatgacaa atttaagaac agatatccgt aacgttgcga
    11941 tcattgccca cgttgaccac ggtaaaacaa ctctcgttga tgaattatta aaacaatcac
    12001 atactcttga tgagcgtaaa gagcttgaag aacgtgcaat ggattcaaat gatatcgaaa
    12061 aagaacgtgg tatcaccatt cttgcaaaaa atacagccgt agcatacaac gatgttcgta
    12121 tcaatattat ggacacacct ggtcacgcgg actttggtgg tgaagttgag cgtattatga
    12181 aaatggttga tggtgttgtt ttagtcgttg atgcctacga aggaacaatg ccacaaacac
    12241 gttttgtttt gaagaaagct cttgaacaaa acttaattcc aatcgttgtt gtaaataaaa
    12301 ttgataagcc gtcagctcgt ccatcagagg ttgttgatga agttcttgaa ctctttattg
    12361 agctcggtgc tgatgatgat caactagatt tccctgttgt ttatgcttca gctatcaatg
    12421 gaacatcttc aatgtcagat gatccttcag atcaagaaaa aacaatggca ccgatttttg
    12481 atactatcat tgatcacatt ccagccccag ttgacaactc ggaagaacca cttcaattcc
    12541 aagtttctct tcttgactac aatgattttg taggacgtat tggtattgga cgtgttttcc
    12601 gcgggactgt caaagttgga gatcaagtta ctctttcaaa acttgatggt acaactaaaa
    12661 acttccgcgt aacaaaactt tttggtttct ttggacttga acgtaaagaa atccaagagg
    12721 ctaaagcggg tgatttaatc gctgtttctg gtatggaaga tatcttcgtt ggtgagacag
    12781 taactccgac agatgctatt gaaccacttc cagttttacg tattgacgag ccaacacttc
    12841 aaatgacttt cttggtgaat aattcaccat ttgcaggtcg cgaaggtaaa tggattacgt
    12901 cacgtaaggt tgaagaacgt cttttagcag aattacaaac agacgtttct ttgcgtgttg
    12961 acccaacaga ttcgccagat aaatggacgg tttcagggcg tggagaatta catttatcta
    13021 tccttattga aacaatgcgt cgtgagggat atgaacttca agtatcacgt ccagaagtta
    13081 tcatcaaaga aattgatggt gttcaatgcg agccgtttga gcgtgttcaa attgatactc
    13141 cagaagaata tcagggtgct attatccaaa gtttgtcaga gcgtaaaggt gatatgcttg
    13201 atatgcagat ggttggtaat ggtcaaacgc gtttgatttt cttgattcct gcacgtggtt
    13261 tgattggtta ttcaacagag tttctttcaa tgacacgtgg atatggtatc atgaatcata
    13321 cttttgacca gtatctaccg gttgttcaag gagaaattgg tggccgtcat cgtggtgcct
    13381 tggtttctat tgaaaatggt aaagcaacta catattcaat tatgcgtatt gaagagcgtg
    13441 gaactatctt tgtaaatcca ggtacagaag tttatgaagg aatgattgtt ggtgagaatt
    13501 ctcgtgataa tgacctcgga gtcaatatta caactgctaa acaaatgaca aatgtccgtt
    13561 cagcaactaa agatcaaact gcagtcatca agacgcctcg tatccttact ttagaagagt
    13621 cacttgaatt tttagcagat gatgagtaca tggaagtaac tcctgagtct attcgtcttc
    13681 gtaaacaaat tttaaataaa gcagcacgtg acaaagctaa taaaaagaaa aaatcagctg
    13741 aataatatcc aaagtatttc ttatttatga ttaataagaa aggagttcta tgttttactt
    13801 aattgttgcc cttttgattg ccttatatta cttttttatg gcaccaaaaa cagtacgtaa
    13861 tactttgaac gctattggtt tagtaggttt ggtagcctta ttacttgtct tggcagttat
    13921 gagttttatt aaaatccttc aattgccagg tgaattgtat attgggctta taatgattcc
    13981 tctaggatat acagcattta aagaaatatt gaatttgtca gaaaagaaga aatagcttat
    14041 gaacttagga ttttgtctcc taagttcttt tgttttgaga aattagagac atatccttta
    14101 ccttgaaaaa gccactgatt aaaggtaaaa taatagggat aataatgata gaatgggacg
    14161 agtaatgaaa acaataacaa catttgaaaa taaaaaagtt ttagtccttg gtttagcacg
    14221 atctggagaa gccgctgcac gtttgttagc taagttagga gcaatagtga cagttaatga
    14281 tggcaaacca tttgatgaaa atccaacagc acagtctttg ttggaagagg gtattaaagt
    14341 ggtttgtggt agtcatcctt tagaattgtt agatgaggat ttttgttaca tgattaaaaa
    14401 tccaggaata ccttataaca atcctatggt caaaaaagca ttagaaaaac aaatccctgt
    14461 tttgactgaa gtggaattag catacttagt ttcagaatct cagctaatag gtattacagg
    14521 ctctaacggg aaaacgacaa cgacaacgat gattgcagaa gtcttaaatg ctggaggtca
    14581 gagaggtttg ttagctggga atatcggctt tcctgctagt gaagttgttc aggctgcgga
    14641 tgataaagat attctagtta tggaattatc aagttttcag ctaatgggag ttaaggaatt
    14701 tcgtcctcat attgcagtaa ttactaattt aatgccaact catttagatt atcatgggtc
    14761 ttttgaagat tatgttgctg caaaatggaa tatccaaaat caaatgtctt catctgattt
    14821 tttggtactt aattttaatc aaggtatttc taaagagtta gctaaaacta ctaaagcaac
    14881 aatcgttcct ttctctacta cggaaaaagt tgatggtgct tacgtacaag acaagcaact
    14941 tttctataaa ggggagaata ttatgttagt agatgacatt ggtgtcccag gaagccataa
    15001 cgtagagaat gctctagcaa ctattgcggt tgctaaacta gctggtatca gtaatcaagt
    15061 tattagagaa actttaagca attttggagg tgttaaacac cgcttgcaat cactcggtaa
    15121 ggttcatggt attagtttct ataacgacag caagtcaact aatatattgg caactcaaaa
    15181 agcattatct ggctttgata atactaaagt tatcctaatt gcaggaggtc ttgatcgcgg
    15241 taatgagttt gatgaattga taccagatat cactggactt aaacatatgg ttgttttagg
    15301 ggaatcggca tctcgagtaa aacgtgctgc acaaaaagca ggagtaactt atagcgatgc
    15361 tttagatgtt agagatgcgg tacataaagc ttatgaggtg gcacaacagg gcgatgttat
    15421 cttgctaagt cctgcaaatg catcatggga catgtataag aatttcgaag tccgtggtga
    15481 tgaattcatt gatactttcg aaagtcttag aggagagtaa ctatggggaa aaaaattgtt
    15541 tttacaggtg gtggtacggt aggtcatgta acactaaacc taatattaat ccccaaattt
    15601 atcaaagatg gttgggaagt acattatatt ggtgataaaa atggcataga acatgaacaa
    15661 attaatcagt ctggacttga tatcacattt cactctatag ctactgggaa actacgtcgc
    15721 tatttttcat ggcaaaatat gttagatgtc tttaaagtag gggttggcgt tcttcaatcg
    15781 attgctatta ttgctaaact tagaccccaa gccttatttt caaaaggggg atttgtttca
    15841 gtacctcctg tagtagctgc acgcctatta aaagttcctg tttttgttca tgaatcagat
    15901 ttatcaatgg ggttagcaaa taagattgct tataaatttg cgactattat gtatacgact
    15961 tttgaacaat ctaaagattt aatcaaaact aagcatattg gtgctgtaac aaaagtcatg
    16021 gattgtaaaa agtcgtttga aaatactgac ttaactagta taaaagaagc cttcgatcca
    16081 aacctaaaga ccttactatt tattggagga tcagcaggtg caaaagtatt caatgatttt
    16141 attactcaaa cacctgagct ggaagaaaaa tataatgtta tcaatatttc aggcgattct
    16201 tctctcaatc gattgaagaa gaacctttat cgcgtggatt atgttacaga cctttatcag
    16261 cctttaatga atttagcaga tgtggttgtt acacgaggtg gctctaatac tatttttgaa
    16321 ttagtagcta tgaaaaaact tcatcttatt atccctcttg gacgtgaagc gagccgaggt
    16381 gaccaattag agaatgctgc ttattttgaa gaaaagggct acgcattgca gttaccagaa
    16441 tctgaattaa acataaatac acttgagaaa cagataaatt tattaatttc taacagtgag
    16501 agttacgaaa aaaacatgtc acaatcatct gaa
//
LOCUS       DATRSD010000052        16079 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRSD010000052 DATRSD010000000
VERSION     DATRSD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.053"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            17..1681
                     /locus_tag="VBD85_001334"
     CDS             17..1681
                     /locus_tag="VBD85_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7133890.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            1770..2693
                     /locus_tag="VBD85_001335"
     CDS             1770..2693
                     /locus_tag="VBD85_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7133891.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            2695..3612
                     /gene="srtC1"
                     /locus_tag="VBD85_001336"
     CDS             2695..3612
                     /gene="srtC1"
                     /locus_tag="VBD85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO7133892.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            3569..4420
                     /gene="srtC2"
                     /locus_tag="VBD85_001337"
     CDS             3569..4420
                     /gene="srtC2"
                     /locus_tag="VBD85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO7133893.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            4502..7174
                     /locus_tag="VBD85_001338"
     CDS             4502..7174
                     /locus_tag="VBD85_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO7133894.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            7228..8018
                     /locus_tag="VBD85_001339"
                     /pseudo
     CDS             7228..8018
                     /locus_tag="VBD85_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            8267..8872
                     /locus_tag="VBD85_001340"
     CDS             8267..8872
                     /locus_tag="VBD85_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7133895.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            9970..10146
                     /locus_tag="VBD85_001341"
     CDS             9970..10146
                     /locus_tag="VBD85_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133896.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            11491..11760
                     /locus_tag="VBD85_001342"
     CDS             11491..11760
                     /locus_tag="VBD85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133897.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            11821..12972
                     /locus_tag="VBD85_001343"
     CDS             11821..12972
                     /locus_tag="VBD85_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7133898.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGTFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            13172..14164
                     /locus_tag="VBD85_001344"
     CDS             13172..14164
                     /locus_tag="VBD85_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7133899.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            14167..14985
                     /locus_tag="VBD85_001345"
     CDS             14167..14985
                     /locus_tag="VBD85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7133900.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQLHITIQGG"
     gene            14987..15772
                     /locus_tag="VBD85_001346"
     CDS             14987..15772
                     /locus_tag="VBD85_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133901.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWTVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
BASE COUNT         5398 a         2523 c         3170 g         4988 t
ORIGIN      
        1 gaggagagaa cttttaatga aattatcgaa gaagttattg ttttcggctg ctgttttaac
       61 aatggtggcg gggtcaactg ttgaaccagt agctcagttt gcgactggaa tgagtattgt
      121 aagagctgca gaagtgtcac aagaacgccc agcgaaaaca acagtaaata tctataaatt
      181 acaagctgat agttataaat cggaaattac ttctaatggt ggtatcgaga ataaagacgg
      241 cgaagtaata tctaactatg ctaaacttgg tgacaatgta aaaggtttgc aaggtgtaca
      301 gtttaaacgt tataaagtca agacggatat ttctgttgat gaattgaaaa aattgacaac
      361 agttgaagca gcagatgcaa aagttggaac gattcttgaa gaaggtgtca gtctacctca
      421 aaaaactaat gctcaaggtt tggtcgtcga tgctctggat tcaaaaagta atgtgagata
      481 cttgtatgta gaagatttaa agaattcacc ttcaaacatt accaaagctt atgctgtacc
      541 gtttgtgttg gaattaccag ttgctaactc tacaggtaca ggtttccttt ctgaaattaa
      601 tatttaccct aaaaacgttg taactgatga accaaaaaca gataaagatg ttaaaaaatt
      661 aggtcaggac gatgcaggtt atacgattgg tgaagaattc aaatggttct tgaaatctac
      721 aatccctgcc aatttaggtg actatgaaaa atttgaaatt actgataaat ttgcagatgg
      781 cttgacttat aaatctgttg gaaaaatcaa gattggttcg aaaacactga atagagatga
      841 gcactacact attgatgaac caacagttga taaccaaaat acattaaaaa ttacgtttaa
      901 accagagaaa tttaaagaaa ttgctgagct acttaaagga atgacccttg ttaaaaatca
      961 agatgctctt gataaagcta ctgcaaatac agatgatgcg gcatttttgg aaattccagt
     1021 tgcatcaact attaatgaaa aagcagtttt aggaaaagca attgaaaata cttttgaact
     1081 tcaatatgac catactcctg ataaagctga caatccaaaa ccatctaatc ctccaagaaa
     1141 accagaagtt catactggtg ggaaacgatt tgtgaagaaa gactcaacag aaacacaaac
     1201 actaggtggt gctgagtttg atttgttggc ttctgatggg acagcagtaa aatggacaga
     1261 tgctcttatt aaagcgaata ctaataaaaa ctatattgct ggagaagctg ttactgggca
     1321 accaatcaaa ttgaaatcac atacagacgg tacgtttgag attaaaggtt tggcttatgc
     1381 agttgatgcg aatgcagagg gtacagcagt aacttacaaa ttaaaagaaa caaaagcacc
     1441 agaaggttat gtaatccctg ataaagaaat cgagtttaca gtatcacaaa catcttataa
     1501 tacaaaacca actgacatca cggttgatag tgctgatgca acacctgata caattaaaaa
     1561 caacaaacgt ccttcaatcc ctaatactgg tggtattggt acggctatct ttgtcgctat
     1621 cggtgctgcg gtgatggctt ttgctgttaa ggggatgaag cgtcgtacaa aagataacta
     1681 aataaaaggc tacttcttaa gtaaccatgt ttaagaaaag agaaatagcc ttatttctct
     1741 ttttgtcgtt tttaaaataa aggaacatca tgaaacaaac attaaaactt atgttttctt
     1801 ttctgttgat gttagggact atgtttggaa ttagccaaac tgttttagcg caagaaactc
     1861 atcagttgac gattgttcat cttgaagcaa gggatattga tcgtccaaat ccacagttgg
     1921 agattgcccc taaagaaggg actccaattg aaggagtact ctatcagttg taccaattaa
     1981 aatcaactga agatggcgat ttgttggcac attggaattc cctaactatc acagaattga
     2041 aaaaacaggc gcagcaggtt tttgaagcca ctactaatca acaaggaaag gctacattta
     2101 accaactacc agatggaatt tattatggtc tggcggttaa agccggtgaa aaaaatcgta
     2161 atgtctcagc tttcttggtt gacttgtctg aggataaagt gatttatcct aaaatcatct
     2221 ggtccacagg tgagttggac ttgcttaaag ttggtgtgga tggtgatacc aaaaaaccac
     2281 tagcaggcgt tgtctttgaa ctttatgaaa agaatggtag gactcctatt cgtgtgaaaa
     2341 atggggtgca ttctcaagat attgacgctg caaaacattt agaaacagat tcatcagggc
     2401 atatcagaat ttccgggctc atccatgggg actatgtctt aaaagaaatc gagacacagt
     2461 caggatatca gatcggacag gcagagactg ctgtgactat tgaaaaatca aaaacagtaa
     2521 cagtaacgat tgaaaataaa aaagttccga cacctaaagt gccatctcga ggaggtctta
     2581 ttcccaaaac aggtgagcaa caggcaatgg cacttgtaat tattggtggt attttaattg
     2641 ctttagcctt acgattacta tcaaaacatc ggaaacatca aaataaggat tagcatggga
     2701 caaaaatcaa aaatatctct agctacgaat attcgtatat ggatttttcg tttaattttc
     2761 ttagcgggtt tccttgtttt ggcatttccc atcgttagtc aggtcatgta ctttcaagcc
     2821 tctcacgcca atattaatgc ttttaaagaa gctgttacca agattgaccg ggtggagatt
     2881 aatcggcgtt tagaacttgc ttatgcttat aacgccagta tagcaggtgc caaaactaat
     2941 ggcgaatatc cagcgcttaa agacccctac tctgctgaac aaaagcaggc aggggtcgtt
     3001 gagtacgccc gcatgcttga agtcaaagaa caaataggtc atgtgattat tccaagaatt
     3061 aatcaggata tccctattta cgctggctct gctgaagaaa atcttcagag gggcgttgga
     3121 catttagagg ggaccagtct tccagtcggt ggtgagtcaa ctcatgccgt tctaactgcc
     3181 catcgagggc taccaacggc caagctattt accaatttag acaaggtaac agtaggtgac
     3241 cgtttttaca ttgaacacat cggcggaaag attgcttatc aggtagacca aatcaaagtt
     3301 atcgcccctg atcagttaga ggatttgtac gtgattcaag gagaagatca cgtcacccta
     3361 ttaacttgca caccttatat gataaatagt catcgcctcc tcgttcgagg caagcgaatt
     3421 ccttatgtgg aaaaaacagt gcagaaagat tcaaagacct tcaggcaaca acaataccta
     3481 acctatgcta tgtgggtagt cgttggactt atcttgctgt cgcttctcat ttggtttaaa
     3541 aagacgaaac agaaaaagcg gagaaagaat gaaaaagcgg ctagtcaaaa tagtcacaat
     3601 aattcgaaat aataaaatca gaaccctcat ttttgtgatg ggaagtctga ttctcttatt
     3661 tccgattgtg agccaggtaa gttactacct tgcttcgcat caaaatatta atcaatttaa
     3721 gcgggaagtc gctaagattg atactaatac ggttgaacga cgcatcgctt tagctaatgc
     3781 ttacaatgag acgttatcaa ggaatccctt gcttatagac ccttttacca gtaagcaaaa
     3841 agaaggtttg agagagtatg ctcgtatgct tgaagttcat gagcaaatag gtcatgtggc
     3901 aatcccaagt attggggttg atattccaat ttatgctgga acatccgaaa ctgtgcttca
     3961 gaaaggtagt gggcatttgg agggaaccag tcttccagtg ggaggtttgt caacccattc
     4021 agtactaact gcccaccgtg gcttgccaac agctaggcta tttaccgact taaataaagt
     4081 taaaaaaggc cagattttct atgtgacgaa catcaaggaa acacttgcct acaaagtcgt
     4141 gtctatcaaa gttgtggatc caacagcttt aagtgaggtt aagattgtca atggtaagga
     4201 ttatataacc ttgctgactt gcacacctta catgatcaat agtcatcgtc tcttggtaaa
     4261 aggagagcgt attccttatg attctaccga ggcggaaaag cacaaagaac aaaccgtaca
     4321 agattatcgt ttgtcactag tgttgaagat actactagta ttattaattg gactcttcat
     4381 cgtgataatg atgagaagat ggatgcaaca tcgtcaataa cgatgttgtg aatggcttac
     4441 ttacttatca aataggtgac taatgatgat tgtgaataat ggttatctag aagggagaaa
     4501 aatgaaaaag agacaaaaaa tatggagagg gttatcagtt actttactaa tcctgtccca
     4561 aattccattt ggtatattgg tacaaggtga aacccaagat accaatcaag cacttggaaa
     4621 agtaattgtt aaaaaaacgg gagacaatgc tacaccatta ggcaaagcga cttttgtgtt
     4681 aaaaaatgac aatgataagt cagaaacaag tcacgaaacg gtagagggtt ctggagaagc
     4741 aacctttgaa aacataaaac ctggagacta cacattaaga gaagaaacag caccaattgg
     4801 ttataaaaaa actgataaaa cctggaaagt taaagttgca gataacggag caacaataat
     4861 cgagggtatg gatgcagata aagcagagaa acgaaaagaa gttttgaatg cccaatatcc
     4921 aaaatcagct atttatgagg atacaaaaga aaattaccca ttagttaatg tagagggttc
     4981 caaagttggt gaacaataca aagcattgaa tccaataaat ggaaaagatg gtcgaagaga
     5041 gattgctgaa ggttggttat caaaaaaaat tacaggggtc aatgatctcg ataagaataa
     5101 atataaaatt gaattaactg ttgagggtaa aaccactgtt gaaacgaaag aacttaatca
     5161 accactagat gtcgttgtgc tattagataa ttcaaatagt atgaataatg aaagagccaa
     5221 taattctcaa agagcattaa aagctgggga agcagttgaa aagctgattg ataaaattac
     5281 atcaaataaa gacaatagag tagctcttgt gacatatgcc tcaaccattt ttgatggtac
     5341 tgaagcgacc gtatcaaagg gagttgccga tcaaaatggt aaagcgctga atgatagtgt
     5401 atcatgggat tatcataaaa ctacttttac agcaactaca cataattaca gttatttaaa
     5461 tttaacaaat gatgctaacg aagttaatat tctaaagtca agaattccaa aggaagcgga
     5521 gcatataaat ggggatcgca cgctctatca atttggtgcg acatttactc aaaaagctct
     5581 aatgaaagca aatgaaattt tagagacaca aagttctaat gctagaaaaa aacttatttt
     5641 tcacgtaact gatggtgtcc ctacgatgtc ttatgccata aattttaatc cttatatatc
     5701 aacatcttac caaaaccagt ttaattcttt tttaaataaa ataccagata gaagtggtat
     5761 tctccaagag gattttataa tcaatggtga tgattatcaa atagtaaaag gagatggaga
     5821 gagttttaaa ctgttttcgg atagaaaagt tcctgttact ggaggaacga cacaagcagc
     5881 ttatcgagta ccgcaaaatc aactctctgt aatgagtaat gagggatatg caattaatag
     5941 tggatatatt tatctctatt ggagagatta caactgggtc tatccatttg atcctaagac
     6001 aaagaaagtt tctgcaacga aacaaatcaa aactcatggt gagccaacaa cattatactt
     6061 taatggaaat ataagaccta aaggttatga catttttact gttgggattg gtgtaaacgg
     6121 agatcctggt gcaactcctc ttgaagctga gaaatttatg caatcaatat caagtaaaac
     6181 agaaaattat actaatgttg atgatacaaa taaaatttat gatgagctaa ataaatactt
     6241 taaaacaatt gttgaggaaa aacattctat tgttgatgga aatgtgactg atcctatggg
     6301 agagatgatt gaattccaat taaaaaatgg tcaaagtttt acacatgatg attacgtttt
     6361 ggttggaaat gatggcagtc aattaaaaaa tggtgtggct cttggtggac caaacagtga
     6421 tgggggaatt ttaaaagatg ttacagtgac ttatgataag acatctcaaa ccatcaaaat
     6481 caatcatttg aacttaggaa gtggacaaaa agtagttctt acctatgatg tacgtttaaa
     6541 agataactat ataagtaaca aattttacaa tacaaataat cgtacaacgc taagtccgaa
     6601 gagtgaaaaa gaaccaaata ctattcgtga tttcccaatt cccaaaattc gtgatgttcg
     6661 tgagtttccg gtactaacca tcagtaatca gaagaaaatg ggtgaggttg aatttattaa
     6721 agttaataaa gacaaacatt cagaatcgct tttgggagct aagtttcaac ttcagataga
     6781 aaaagatttt tctgggtata agcaatttgt tccagaggga agtgatgtta caacaaagaa
     6841 tgatggtaaa atttatttta aagcacttca agatggtaac tataaattat atgaaatttc
     6901 aagtccagat ggctatatag aggttaaaac gaaacctgtt gtgacattta caattcaaaa
     6961 tggagaagtt acgaacctga aagcagatcc aaatgctaat aaaaatcaaa tcgggtatct
     7021 tgaaggaaat ggtaaacatc ttattaccaa cactcccaaa cgcccaccag gtgtttttcc
     7081 taaaacaggg ggaattggta caattgtcta tatattagtt ggttctactt ttatgatact
     7141 taccatttgt tctttccgtc gtaaacaatt gtaaggtgtc gttgaaatta ttaaataata
     7201 gaaaatgact agttttggcc tttccctatt gtcagtcaga ttagttatta tcaagcttct
     7261 catgccaata ttaatgcctt taaaagaaca gttacaacca ttgaccgtac ggagattcaa
     7321 cgacggttag gtctggctaa agcctacaat gctagtattt ctggaacaag tagtcagtcg
     7381 actcaatctg tgctgagaga ttcttattct gaggagcaaa aaaggcaggg tggactgaat
     7441 acgctaggat gttagaagtc agagagcagg ttgaccatgt gatgattcca aaaatcaatc
     7501 aggatttacc aatctacgct ggttcagaag aggacaatct gcaacgggga gttggtcatc
     7561 tagaagggat aagtttgccg attggagggg cttctacaca tgcggtcttg agcggtcaaa
     7621 gaggtatgcc agctgctcgg ttgtttgcgg atttggataa gatgaaaaaa ggtgattatt
     7681 tttatgttac caatctgaaa gaaaccttgg cttatcaagt ggatcgtatc atggtgattg
     7741 aacctagcca attggatgcc gtgagcattg aagaggataa agattatgtt acccttctga
     7801 cctgtacacc ttatatgggc tctttgtcaa ctgtaatggg tgacttatca ttaacaacga
     7861 gagagaatca gcttggttct ctctcttttt ggatgttcaa agcgatgaga attttgcttt
     7921 taaaattttt aaagttgcga aagccaaagg cttgtcgctt gatgtctttg ataagcttgt
     7981 tggtcgcttc taatttggca ttggaaaggg atagctgagg gcgtttgtga tgtaagtttt
     8041 gtacttcatg aaggtcctaa agaccatttt aaaagcagaa ttgccagttg ttacgttatc
     8101 atctatgaag ctaaagaatt cagttgttcg cttttcttag aaatgaaaga gtatgagctg
     8161 ataaaggtcg ttataatagc ggagctcatc tgagaaaact tttatacctc aaagtcagtc
     8221 tagctttgat atcatgaagc cattaggagt tattccttat cttttagtgg cgcgcgatcc
     8281 atatagtgat agatcgagat atttagatcc aaaagttcta tcatcctctt ttggcgcctt
     8341 ttttccagca gataatatta aggtagcttg gtctaacaac tccagcactt tatttacacc
     8401 acctattaat gcaaactaca ccactcagat tcaagctatt gggacaacga ttaagtcaca
     8461 aattccggaa tcgattttga cggttacgga taaaaagagc aggaagttca gcattaacaa
     8521 gattgacgaa gctaaagaag gcttagtagg tgcgaccttc accttgtcta aacgcacaac
     8581 agtagcggca gatcatcaag tacaaggaga tttcattcct gtcagcaaag agacgacagt
     8641 cggtcggaca acccttacct ttgacaacct taaacctgga ttttatgacc ttaaagaaac
     8701 gaaagcgccg aatgcttacg tacttgatcc taagacttat gttgtggtcg ttcaaaattc
     8761 aggaaaaacg acaattgtgg atgaagctaa cttcaaagag gctgattacc caatggctga
     8821 taataccagc catgtggagt gcgtagcgtt gctacaacga agcaaagggt aaaaatcctt
     8881 tattttaagc actttttcaa gcattttgtc tttattgaaa agagtgattt taacataaaa
     8941 aaggtattaa aaaacatatt gacgtgaccg tttgttttga agtggcttgc gtagacaaaa
     9001 aaatagatac gtcagataaa tttctggcat tacgagaaca tttttagagt gttctctttt
     9061 tttagtttac ggaggaaaaa tatatatgga aaaacaggat tcacgagttc tcatccattg
     9121 ggaggggaat tctggggaca agctcattga acaccaaacc agcgcaacgg ggtggtacta
     9181 ccaagtcgat cgtagcttta gtcaaccaaa aggggaaccg cccagaatga tccaaaggca
     9241 ctagaaagtg tccgtaatga ttcgatttcg ggcggtgatg atgtcatggg ttatgcttat
     9301 agcaaatgta cttggggagt tgcggcacga attaatcagt gggactgaaa ctcaaaggtt
     9361 gaaatggtga gaagattacc attaccagtt caatgggaaa tggtcaggat tgggttggaa
     9421 cagccgaaag actagatggt gaaactgata cagttccaaa agaaggtact attctctctt
     9481 tttaggaaag tagttatggt tcgtatatag gctacggaac tatatctttc gtcacattac
     9541 atctacagat agtaccatga attttgctta tatgaccaag taaagtgagg atatactaac
     9601 aaatgaaata tttattatcg tatttgtcca ttttatcgaa aagtttgcat attatcatta
     9661 tgtttgataa gatgcaaata taatgatagt aggagctaaa tatggatatt taaaaaatca
     9721 agagtatcct aagtgctttc cattttgaaa ttcaaatata gctaatagtt ctagaacttc
     9781 taattgtttt tcgtcgacga tatgaatttt caatcttaac tgttaggatt ccacctccct
     9841 ttggttaaag aaaaaaggtc aggtcgttta gataactttg tcaaacaagc tcaagctatc
     9901 taaaaatagt ttgaaatggg cattactcta gtttttaata agctatctga tgagcagaag
     9961 gagaagttaa tgcatgttgg gaagtcttat tttgactatc aagaaaatgc tcttatccca
    10021 caattaggtt ttctatattc taaattaact aaaaaaattg aacttgataa tcggttgtct
    10081 ccgactgaac aaaagttatt gattacctta ttattacata ctaaaggttt aatcattgat
    10141 atgtaagaag taagtcagct aaccgatctt tctattctaa aacttatatt gttgctttag
    10201 aaattttaaa gagcgtggat ggcttcataa taaacagaaa tcttaccaat ttgcgaagcc
    10261 aaaaaatatg atatttgaag agtccaaaga tctaatagat agtccagtta gagaagcgtt
    10321 gattataagt gataaggatt ttcaaaaatt aaaacaagag ctattatttt aaccgactta
    10381 ttttaaagac ttatcatatc taggcttgct tgatgattcg gaaaaatacg gagactatac
    10441 tatttcaagg aaaagataca aaagtttcga atcaagtctt caactataca tccttcaaag
    10501 tcatcggcta gagatttgga attatgaacc aatccctttg attactagaa aaataaatag
    10561 cttggagagg ctaacgtgac actggttgat ccaatctcgc tttatttaac actaagaatg
    10621 atgaagaccc tcgtattgaa gaagaagttg agcagctaga agataagatt ttgagaatat
    10681 aatcgttttt tcgtgctaca ctaacttgtc agggttgcaa cagacaaaaa aataatttag
    10741 gagggattcg ttatgcgtac aaagtatata ggatatgaaa ttgttttaag gggtggttat
    10801 taatatggat aagaaaaagg ttgtttcaac tatcgtatct acagggtttc ttatagtttc
    10861 aatatcaaat ttttttagag agctatcacc tatacttttt ctcttgaata ttattggatt
    10921 tttctgcttc ctattattat tttctgccaa cataaataaa ttgttcttaa aacgatagcc
    10981 gttatatagg atatggtatg tatgcatttg gtgaaattat aggtaaaacg aatggaatag
    11041 tacttactcc tcaaagctat cgtgagaggt ataaaaggat atcgaggagt cactagatag
    11101 ttggatctca ttgaaaaaat ttgtttaaag tatctcatat aagcataaaa cgaggaatca
    11161 atcaaaattt cttgattgat tccttgtttt ttggctcttt gtcaactgta gtgggttgat
    11221 gaaaaattat attctggaga aaaccaaatt ggttttctcc tttttgatat tcaaagtgat
    11281 atgaattctc tttttaaaat attcaaagtt tctaaagcta aatgtttgtg cttgatgatt
    11341 ttgatgagat tattggttgc tcaagtttgg tgtttgaata aggcgattca aggacattga
    11401 taaccttatc tctgtcttta aaaaatgtcc taaaaacagt ttaaaagatt ggagtgacac
    11461 tagagaaagt gttttaaaca gggtaaagaa atggtgagct tgcttctctt gaaagtaaag
    11521 caggaggagt tgatagagtt tcataatgtt ttttatagag aagcgatatc ttcttatctt
    11581 gaaacagaaa attttgagaa ggctaaagtg actgaagaga attaccaagt ttttttagga
    11641 atccgtactt atcagaagag ttataactat caagatcata gtgagaatct aaaaggaaat
    11701 attgaggtag caatgatagg agttgaaaat aatattagcg acgaacatat tttttgctag
    11761 aatatataaa atgtctttga aataatttag ccctgtgtta tgatattaag gaggttattt
    11821 atggtacgta aatttttaaa atcgctccta tctctctttc ttatagcagt aatcgccacc
    11881 ggtatttcag tggcatgttt cttttttatt ccagaaaata aagggaatat tacaccgatt
    11941 ttattgcatc gctttatgcg aaaaaataat gttaatggaa tgatgattgt tagcgataat
    12001 actggtaaac cgattaccat ctcgcatggg attaaccgtg gggaagttga aacggatatt
    12061 gaaaataata aattatttcc aatggcttct ctccaaaaac tcatgacagg aattattatc
    12121 caaagattaa ttgatcaaga tgtcttgagt gaagacgacc gattgtctca gttctttccc
    12181 caagtaaaag gtagtaatag tattaccatc caccaattgt tgacccatac gagtgggtta
    12241 agagaaaaag gtgttaaggt gtctccttat ctaaaaaatg agagagaaca attacaattt
    12301 tgcttaaaac actataactt tgttaataag aaatcgtggt attattctaa tatcaacttt
    12361 agtttcctaa caggtatagc cactcaagtt acgggaagga cctacgctga acttgttgat
    12421 gatgtgataa aaaatccttt gcgattagat gatacccaaa gttaccaatc agttgttaat
    12481 catgacttag ttagcccaat gcgaaagaac ggcaagttaa ataaaattaa tatctttaat
    12541 caagtatcca cagcatatgg agctggagat tttttcacaa ctccgctgaa cttttgggtg
    12601 ttgatgcggt ctttttctaa agggtacttt ttcccaacgg atgaatatac caaacatcaa
    12661 aatgatgcga tatcacatta ttatggtggg ctttacatgc atggtcgtat agttaattct
    12721 aatgggacct ttttccagta ttcaagtttt ggttatgcag atctaaagac gcatgataca
    12781 atggtgcttt ttatgaataa caaagtctat agtgatgcta gtcacgtggc accaaaagca
    12841 tttgaatatt atcaaaaatt tatgttcctt aataagattt tccacttagt cttttacttt
    12901 gtattttctt tctttttaat gcttttaata cgtcattcgt ttaggaaaag aaggtataag
    12961 aaacgtctct aaatcacatg aataaaaaag aaggctaagt cggccttctt ttttattcaa
    13021 tattaagcag taacaccatc taagaaaacg tttgcattga atttttaatt gtgctataat
    13081 aaatagctgt taatcattat tatagtttaa taatctaact gtcgagctga tttaagctat
    13141 aaaaatactg ttgagtaaaa gaggtattaa tatgtcaatg attgaagttt ctcaccttca
    13201 gaaaaatttt ataaaaaccg ttaaagcacc agggttaaaa ggagcatttc aatcatttct
    13261 tcgtcctgaa aaacacacct ttgaagctgt taaagattta acatttgatg tgcctaaagg
    13321 acaaattttg ggatttattg gagcgaacgg tgcaggaaaa tctacaacaa ttaaaatgtt
    13381 aactgggatt ttaaaaccaa cttcgggctt ttgccgtatt gatggcaaat tgccacaaga
    13441 aaaccgacaa aattatgtta aggatattgg agtcgttttt gggcaacgta cgcagttatg
    13501 gtgggattta gctctacaag aaacctatac agtgttaaaa gaaatctatg acgttcctga
    13561 taaagaattt cgcaaacgca tggctttttt aaatgaggtt ttggaactaa atgactttat
    13621 aaaggatcct gttagaactt tgtctctcgg acagcgtatg agagcggata tcgcagcttc
    13681 tttattacat aacccaaagg ttcttttctt agatgaaccc accattggct tggacgtttc
    13741 agtgaaagat aatatccgac gtgcaatcac acaaattaat caagaagaag agacaacgat
    13801 tttactaacg acacatgacc tcagtgatat tgaacaactt tgtcatcgta tttttatgat
    13861 tgatagagga caagaaatat ttgatggcac agtttctcag ctaaaagaga catttggaaa
    13921 gatgaaaact ctttcttttg atttgcgccc aggacaagag catatcagct ctagtttaat
    13981 tggaaagtcg gagattaata ttaaacgtaa tgacttagtt ttagatattc agtatgatag
    14041 ttctcgctac caaactgctg acataattca gcagacatta gcagattttt ctgtacgtga
    14101 ccttaaaatg acagatgcgg atattgaaga tatcattcgt cgcttctata gaaatgagct
    14161 ataaggatga acagaatttg gagacgttat aaacctttta ttagtacagg aatccaagga
    14221 ttaatcacat atcgtgttga tttcatcctt tataggatag gtgatgtgat aggagcattt
    14281 gttgcatttt atctatggaa agcagttttc gattcctcaa gtcaatcctt aatccaaggt
    14341 tttcagctgt cagatatgat tctctatatt attatgagtt ttgtgactaa tttattaaca
    14401 agaactgata gttcatttat gataggtgac gaagtaaaag acggttcaat tattatgcga
    14461 ctcttacgtc cagttcactt tgctgcttcc taccttttca tggagattgg ttctagatgg
    14521 ctcatctttt taagtatagg ggtaccgttt ttgcttgtta ttacgggggt acgcctcttc
    14581 ttggggactg atttgattca agctatagtg ttagtggtat tttatattat tagcattatt
    14641 ttagcttttt tgattaactt cttttttaat atttgttttg gcttctcggc atttgttttt
    14701 aaaaatttat gggggtcaaa cctgttaaaa aattcgttag tcgcatttat gtcggggagc
    14761 ttgattcctc ttactttttt tccgaaaatc gttgctgata ttttaggatt tttaccattt
    14821 tcatctttaa tttacacacc agtaatgatt attattggta aatatgatgg cagtcaaatt
    14881 gtgcaagcct tacttttaca aatattttgg ctaatagtaa tggtagcttt atctcaatta
    14941 atatggaaaa aagtacagtt gcatatcact atccaaggag gttgagatga caaaatatca
    15001 gcgaatgcac tttattttta ttaaacaata catgaagcaa atcatggagt ataaaataga
    15061 cttttttgta ggcgttttag gagtattttt aacacaaggg ttaaatcttt tatttctgaa
    15121 tgttcttttt caacatatcc cttctttaga aggatggact tttcagcaaa tagcttttat
    15181 ttatggtttt tctctcctcc caaaaggaat tgatcacctt ttctttgata acttgtgggc
    15241 tcttggtcaa aggcttattc gaaaaggaga atttgataag tatttaacca ggccgatcag
    15301 tcctttattt catgtcctag ttgaaacctt tcaagtagat gcattggggg agttattggt
    15361 tggttttatt ctgttatcta ccacagtatc tagtataagc tggaccgtgc caaaagtatt
    15421 attatttata tttattatcc cctttgcaac tttgatttat acctccttga agattgctac
    15481 gtcaagtatc gctttttgga ccaaacaatc tggtgctgtt atctatattt tttatatgtt
    15541 taatgacttt gctaagtatc ctgtcgcaat atataataat ctattgcgtt ggatcattag
    15601 ttttgtcatt ccatttgctt ttacagctta ttatcccgct gcttacttct tacaagatag
    15661 aaatgtctat tttaatattg gtggggttat cttgatttcc ttgatttctt ttatggtatc
    15721 actaatactt tggcataaag gagtagaagt atatgagagt gcgggttctt gattagtaag
    15781 gcaagccatt aatttttttg agtgtttaga agttttaggc ttactaactt agctttgtta
    15841 gtgatgatat actcccctta tagtgtctag tgaatttttg tttttttact gtccactata
    15901 aggggagtat atcaggtgaa gagggactat tagtgttgag aataatgtcc ctcatagaac
    15961 aataattacc atatctttac caaaataaag ttaaaaacgc tcttagatgt gctttctaag
    16021 agcgtttttt ggtctataaa aggtaagcaa aaagcagatg tcatttatga aagtgttga
//
LOCUS       DATRSD010000053        15075 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRSD010000053 DATRSD010000000
VERSION     DATRSD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.054"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            152..1345
                     /locus_tag="VBD85_001347"
     CDS             152..1345
                     /locus_tag="VBD85_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7133902.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQNYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KPKTI"
     gene            complement(1540..2064)
                     /locus_tag="VBD85_001348"
     CDS             complement(1540..2064)
                     /locus_tag="VBD85_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7133903.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2196..3059
                     /locus_tag="VBD85_001349"
     CDS             2196..3059
                     /locus_tag="VBD85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7133904.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            3144..3986
                     /locus_tag="VBD85_001350"
     CDS             3144..3986
                     /locus_tag="VBD85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7133905.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHIN
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDQSLEALGLDCID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLKNGHV
                     KPAVNQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYGVSVA
                     QLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFSNFPV
                     FSEK"
     gene            4096..5132
                     /locus_tag="VBD85_001351"
                     /pseudo
     CDS             4096..5132
                     /locus_tag="VBD85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            5148..5537
                     /locus_tag="VBD85_001352"
     CDS             5148..5537
                     /locus_tag="VBD85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7133906.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDERDNLEDRLQGLQEAL
                     NRLNHKIDNYQNKVVPREQELFDPTTK"
     gene            5547..5942
                     /locus_tag="VBD85_001353"
     CDS             5547..5942
                     /locus_tag="VBD85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7133907.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIGQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEEGKEAVSLKPGDVIVTDKGVRHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSDEEYSRLG"
     gene            5966..6283
                     /locus_tag="VBD85_001354"
     CDS             5966..6283
                     /locus_tag="VBD85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO7133908.1"
                     /translation="MKEKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRS
                     IITISALISGGNLEQLEHHLQFAKQNGVTKEEIADIITHLAFYVGWPKAWSAFNKAKE
                     IWI"
     gene            6370..6531
                     /locus_tag="VBD85_001355"
     CDS             6370..6531
                     /locus_tag="VBD85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7133909.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDV"
     gene            6531..6653
                     /locus_tag="VBD85_001356"
     CDS             6531..6653
                     /locus_tag="VBD85_001356"
                     /inference="COORDINATES: protein motif:HMM:NF021585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7133910.1"
                     /translation="MLRDYLEDMLKKEGKEVYPFSRLASHSANRWYLYETPWRY"
     gene            6892..7383
                     /locus_tag="VBD85_001357"
     CDS             6892..7383
                     /locus_tag="VBD85_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7133911.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYRVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNKSKT
                     NLI"
     gene            complement(7427..7816)
                     /locus_tag="VBD85_001358"
     CDS             complement(7427..7816)
                     /locus_tag="VBD85_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7133912.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
     gene            complement(7829..10996)
                     /locus_tag="VBD85_001359"
     CDS             complement(7829..10996)
                     /locus_tag="VBD85_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO7133913.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNNNSSSSKTESVVSDHKQVPKAKPGV
                     TQEASNSSNDASKVEVPKQDTASKKETIETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLSNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKYKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGAHHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPDLQTIREEAFK
                     RNHLKEVKGSSTLSQIAFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPVLNVDEDNEGYHALAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLIPKTPKGYLNTVPNYRKKQVEKNSKPVDYKTPIFD
                     KVLPNEKVDGDRVSKGHNIKAETNNSVAVTPTRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            complement(11059..13227)
                     /gene="nrdE"
                     /locus_tag="VBD85_001360"
     CDS             complement(11059..13227)
                     /gene="nrdE"
                     /locus_tag="VBD85_001360"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7133914.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFASDKEAVEQYMIEHVEPNT
                     MAFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFTFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGPEAIINIAAAAQKHVDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDDMLDDFTSSDLEDCQSCMI"
     gene            complement(13229..13642)
                     /gene="nrdI"
                     /locus_tag="VBD85_001361"
     CDS             complement(13229..13642)
                     /gene="nrdI"
                     /locus_tag="VBD85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7133915.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLGCSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            complement(13643..14653)
                     /gene="nrdF"
                     /locus_tag="VBD85_001362"
     CDS             complement(13643..14653)
                     /gene="nrdF"
                     /locus_tag="VBD85_001362"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7133916.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
BASE COUNT         4915 a         2719 c         2485 g         4956 t
ORIGIN      
        1 ctaatctgct ttttttttat atataatttg actttttcga ttttttgaat catatttggt
       61 taaaaaagta tcgactacct aattattctg ttgactttaa agaaatattc tctaaaatag
      121 ataacatatt aaatcaaagg tggtaactca tatgattgat tttctactgt tgaaatcatt
      181 acacagtctt ctaggtctaa ctattactgt ttgtgatcaa aatttttctg ttatcagaga
      241 gtataaatcc gagaaaacta tttcactttt ttacaatcat taccttattt taagtaactt
      301 tagtaaaact caacatgatt ttttatttca ttatggctct ttaggagaac tttttcttgt
      361 ccaccatatt caacaatatt atattatcat cggtccctgg cgtagcaatg ttattgaccc
      421 tttacttctt aagaaaaagc tgactgaaac acaaatcaat gctagtgaac aaaactactt
      481 tattgataga ctatcccaat tacctttctt ctcacttagt caaatccgag aattactaat
      541 agtaaccaat tattgtctaa ctggggttgt gaaagataaa ttatcagagc ccttacatta
      601 ctacacgaaa gggtggagca actcctttga tctagataaa attaagcaat tttctaaaca
      661 gaatatgagt tcttacaaat atcagtatca ttttgaaaat aatattctca aagctgttaa
      721 atcaggtagt gaatttcttt tgaaagaaac agtggaacaa tttagcaatt ctatcgtacc
      781 tataatcagt ggagatgaat tgcgatctga aaaaaactac tccatcatga tttatgatcg
      841 tctctcacag gctaccatcc aagcaggact tgatatcgaa acagcttatc gagcacgaga
      901 tcgttttata aaagaaaacg agtcaactat aagtctaaat gaagttttaa aattacgtga
      961 tactgctatc ttattctata ctcaacaagt tcattcttta aaaaaacatc tcgaaacccc
     1021 tcattctcaa actattgtcg cagtgattcg atatcttgaa aataatctaa atcgctttat
     1081 taaaacagaa gaaatagcta aagaatgtca catgagtgaa tcgaagttac gaaaactatt
     1141 taagcaagaa aaacacatca ctattcaaca atattttcta aacttaaaaa tcgaagctgc
     1201 taagcaatta ctagatgaaa ataagaaagt agaagaagtt tccaatttac ttggattttc
     1261 cacttcttct aatttttcaa ggacatttaa aaaaatagtg ggaattagcc cactagaata
     1321 taagcaaaag cctaagacaa tatagtaatc gaaaaatatt cattttgctt atggtgtcat
     1381 caacaatccc aaagaaactc tttcatcaag gggacttttt tatctcccga attatactca
     1441 attaatctaa taacttcaac tcgctatatt agacaatcaa tccattcatc acctctgata
     1501 acgctaatga aataagagaa tctcccaact ctctataatt tacctctcta ccatagttaa
     1561 ataatatttt agtaaatctt caacagtcat tttctgtctt tgatgaagcc accactttaa
     1621 ggtctcgata aaactggata aaagaaattg ttttaagaaa tcttcaggaa tgtccacctt
     1681 tgtaatatac tcctcacgca atctagggta aacatcgtgt tctaattcac ttctaaagcg
     1741 taataaaaaa tagggatcgt cagataataa taacgtagcg atactatctt gattttgttc
     1801 aaaatgtttt aagatatgaa ctaaatactc ttcaaacgta acatctctac cctgcttaaa
     1861 taagtggtga aaaaggtctt cacagagctc cttgagtaac acttctttgc tttcatagtg
     1921 agagtaaaaa gttgatcttc ctacatttgc aagtccgatt acatcttgaa ccgttatttt
     1981 agaataatca ttttcctgta gcaagcttat aaaagcatta taaatcgcta cttttgtttt
     2041 ctgaatttgt ctatctttta ccatatagga cactttctaa gttttgttca gtattgaaca
     2101 agtgttcttt tttgtttcta gcaattctct tttatctcct agataataga agaaagaatc
     2161 acactacaat accagtataa caaaaggaga aaaatatgac atcaaacaaa aaagtggcta
     2221 tcgcttttat attaaatatc agcttttccg tccttgaatt catctttgga agtctattct
     2281 tctcaggtgc tatcttagcc gatgctgttc atgattttgg tgatgcaata gctattggca
     2341 tctcagctac tttagaaaaa aaatctaaga aagatgaaga tacgatattt tcattaggtt
     2401 ataaacgttt tagcctttta ggagctctta tcacaagtct aattcttata agtggatcta
     2461 tcttagtcat gatagaaaat attccaaaat tgtggcatcc tactcctgtt aattatcacg
     2521 gcatgtttat tctagctgtc atcgctatta tcattaatgg tctagctagt tttattctcc
     2581 attctggtca aagtaaacat gaggaaatat taagccttca ttttttggaa gatattctag
     2641 ggtggttagc tattattgtt atatctctta tcttaaattg gaaaccgttg tatatccttg
     2701 atccattatt atcagttgcc atatctactt tcatcttatc taaagcactt cctaaattac
     2761 tatcaaccct gaaacttttt ttagatggtg ttccagattc catagattac gctgcattac
     2821 atgatgaact taagggactt tcgcaagtaa gatctatcaa tcaactcaat atctggtcaa
     2881 tggatggtat tgataatcga gcaatcattc actgttgctt aaatcaattg atatctgaaa
     2941 aagattgtaa aagagctatt cgaacgattt gccaacatta taagataaac gatgttactg
     3001 ttgaaatcga ttattcttta agagaacatc aaaatcattg caaaccatta aaaaactaac
     3061 ctgagtgtta gttttttcct tgacttgaag ttaactctaa gtgctaagat taggtcagta
     3121 ctaatgaaag aaggaaaata aagatgacag atacaatgat attaaatgac aatcataaaa
     3181 taccaacttt aggtttaggt acgtggatga tagataatga tagggtagct caagttgtta
     3241 aagatgctat cgaattaggt taccgccaca ttaatactgc acaagcatac aacaatgaga
     3301 aaggtgtcgg tgtagggatt aaagaatctg gtattacacg tgaatcttta tttgttacga
     3361 ctaaaattgc cgctgaacat aaagattatg aaagtgctac aaaatcaatt gatcaatcac
     3421 tagaagcttt agggcttgat tgtattgatt taatgattat tcatagtcca cagccttgga
     3481 aggaatggcg tgaaacagat aaacattttg accaaggaaa tttagaagct tggagagctt
     3541 tagaggatgc tcaaaaatct ggtaaggtaa aatccatcgg tgtctcaaac tttttagaaa
     3601 aagacttaaa aaatggtcat gttaaacctg ctgtcaatca aatcttagct cacatcggaa
     3661 atacaccttt cgacttgatt gattactgtc aaagtaaagg tattcaagtt gaagcttatt
     3721 caccaattgc acatggacaa gcattgaaat ctgatggtat tcaaaaaatg gctgaaaaat
     3781 atggggttag tgttgctcaa ttatgtattc aatatttact acaattaaac ctgatcgttt
     3841 taccaaaggc ttctagtaaa gaacatttac agtctaatct agactttgac tttgtaatta
     3901 gtgacgaaga tatgtcgata ttaaaatcac ttatgtttga cgattatggg gaattttcta
     3961 acttccctgt ttttagtgaa aaatgaaacc atatgcggat ttttacagaa gtttaatttt
     4021 caaaattttt aattttttct tgacttgaag ttaactctaa gagttataat gaagttatag
     4081 aaaagaggtt aggttatgaa agttgctaca tttattgaac ctggcaaaat ggttataact
     4141 gatacaccaa aaccagtcat tgaacaagag acagatgctg ttatcaaaat tgttagagcc
     4201 tgtgtttgtg gttcagactt gtggtggtat cgtggtattt caaaacgtga aagtggctct
     4261 tttgcaggtc atgaggctat tggtatcgtt gaggaagttg gtactaaagt aactgacgtg
     4321 tcaaaaggtg attttgttat tgttcccttt acacatggct gtggccaatg tccgtcttgt
     4381 aaggctggat ttgatggaaa ttttacaaat catcaagctg caaaaaatgt agataccaag
     4441 gtcaatatct acgttatact aatgcaaact gggcattggt taagatccca ggacaacctt
     4501 ctgattatga taatgagact cttaactctc tgctcacctt atcagatgta atggcaactg
     4561 gttatcatgc tgcagtaact gcagaagtta aagaaggtga tacggtagtt gtcatgggag
     4621 atggtgctgt tggactctgt ggtgttatcg ctgctaaaat gttaggtgct aactgtatca
     4681 ttgcaatgag tcgtcacaaa gataggcaag aactagcatt gacttttggt gcaacggata
     4741 ttgttgaaga acggggtgac gaagccgtta aacgtgtttt agatttaacc aatcaagcag
     4801 gtgccgatgc tattttagaa tgtgttggta cagagcagtc tgttgataca gctacccaaa
     4861 ttgctaggcc tggtgctgtc ataggacgtg ttggtatccc acaaaatcca gacatgaata
     4921 ccaataattt attctggaaa aatatcggcc ttagaggtgg cattgcttct gtaacaacat
     4981 ttgataaatc tgtcctttta gatgctgttc taactcataa aattaaccca ggcttagttt
     5041 ttacaaaatc ctttgtatta gatgatattc aaaaggctta tgaagcaatg gataaacgtg
     5101 acgctatcaa atccttagtg attgttgact gaatatcgag gttaaaaatg catatcaaag
     5161 aagttagtaa aaagactggc attacgtcag ataccatcag gtattatgag agaattaatc
     5221 ttattccccc tattcatcgg agtgaatcag gaattcgtga gttcacacaa aacgatattg
     5281 atattttaga atttgtccgc tattttagag gcgcaggtgt ttccgttgaa agtctcattg
     5341 attatattca tttggttgaa aaaggcgatt ccactatccc agctaggcta gaaattttaa
     5401 aagatgaacg cgacaatctt gaagataggc ttcaaggcct ccaagaagca cttaatcgtc
     5461 taaatcacaa aatagacaat tatcaaaata aagttgttcc tagagaacaa gaactttttg
     5521 atccaacaac gaaatgagga aatcttatgc aagaaacaac taagtctatc tttcctaagg
     5581 gtgaaaaaaa tccatatggt gaattcttta ttgggcaatc ctatctcgca gcattagcaa
     5641 aatctccaga tgggaatgtt tcagttggaa atgtaacctt tgaagcaggc tgtcgtaata
     5701 attggcatgt tcatttagac ggttatcaaa tccttctcgt tacagaaggt tcaggttggt
     5761 accaagaaga aggaaaagag gcagtgtcat taaaaccagg cgacgttatc gttacagata
     5821 aaggtgttag acattggcat ggtgctaaaa aagattctga atttgcacat attgcgatta
     5881 cagctggtaa gagtgagttt tatgaagctg ttagtgatga agaatattca agactaggtt
     5941 aaattcaagt aaggaggaat ccattatgaa agaaaaacaa acagccggac gtcgtcaatt
     6001 agaagagttt gcacctgaat ttgccagata taatgatgat attttatttg gtgaggtttg
     6061 ggcaaaagag gatcatttaa cagataaaac acgttctatt attactatat ctgccttaat
     6121 tagcggagga aatcttgaac agttagagca tcatcttcaa tttgccaaac aaaacggagt
     6181 tactaaagaa gaaattgctg acattatcac tcaccttgct ttttatgttg gatggccaaa
     6241 agcttggtca gcctttaata aagctaagga aatttggata taaaataaca gacatagcaa
     6301 gggagatact ttcccaaaag ccaaaaagaa agatcaacca attattaaac caactattag
     6361 gagaatgata tgcttactat tcaagaaatg aaagatacaa cctttgaatt acaagaaaat
     6421 tttagaaggt taaattatcc tattaaacaa gttgctaaag acttgcaact taatatcagt
     6481 gaagttgaat ctcttttaag tcttgatgtt acttatcctg gagatgtttg atgctgagag
     6541 attatctaga agatatgttg aaaaaagagg gtaaagaagt ttatccattt tctagattag
     6601 catctcattc agcaaatcgt tggtaccttt atgaaacacc atggcgttat taaagactaa
     6661 agattaatcc atcatcttta gagaaataaa caaaaatagt caatagattc tttttagaaa
     6721 aaagctataa aacaagagcc tcttagagaa tactctacat cataagattt cttctttgaa
     6781 gcatagcatt ccaaaagaaa attatgattg ctaacaagac aattattcta aaaagaaact
     6841 attttaacga ctctataatt acttaaggaa tcataaagaa taaaggtttt tatgctatca
     6901 tttcaaattg ttgaaaagcc agcaatgata cttgctggag taactttaga aaatgtaaag
     6961 tcaaatcaag aatgtcctaa actttgggaa aaattacata aacaatataa tccatcaaaa
     7021 ctaaaagaaa aaggtatcca gcaagccatt ggcatttgta aaacacaacc tgattttcgt
     7081 ttcgattaca gcgccactta tcgagtagag acaagtgtcc aagccccaaa agggttagaa
     7141 attattagga tcccaagcgc tacctatgca gtcatttctg ttaaaggtcc aatgccatcg
     7201 agtcttcaag aaacatggcg aaaaataata caaggatttt ttcaagagaa taatctaaaa
     7261 ccagctaact cgccaaactt agaaatttat tcatctcaac atccacaaga tactgattat
     7321 caaatggaaa tttggcttgc tatcgcagat attactaata aatcgaaaac aaacttaatc
     7381 tagccttgct taatataaaa aagccccttt ttgaggcttt tttatattaa gtgatttctt
     7441 tacctgcatt agcaccagat tgtaaattaa aaatacgttc gacatgaata ataccaactg
     7501 ctttcggaac ccccatgcgt ccttctgccc attttttagc tgcttcgtaa taagcacctt
     7561 ctgtttgaat ttcagcagtt cccacaaacc tgtaacctaa aagacgttca cgatcaacaa
     7621 aggcaatttc aattttccca ttatcttcaa tgttaatacg agtttgacca tctgtatttt
     7681 cattataaat aaacgtttta tcatcccaga gacgcataga acgttttggg ccaatattgg
     7741 gttgaccttt agcatcaacc gtcgcgacca ttgctaactg actattaata atctctttca
     7801 tttcttgtgt aatcatatta atttctcctt attttcttgc tcgttttcct ttttttccag
     7861 cagttactag gaaaagcaaa cataaactaa cgtatcctag aatctcgtat ataaaattat
     7921 ttttagaact tgtttgaggt aaatttacat cagactgtga cttatgtaat tgctgctcgg
     7981 accttgttgg tgttacagct acagaattat tagtctccgc ctttatatta tgacctttag
     8041 acactctatc gccgtctacc ttttcattag gtaaaacctt atcaaaaatc ggcgttttat
     8101 aatcaactgg ttttgaattt ttctccactt gttttttacg ataatttggg actgtattta
     8161 ggtacccctt aggtgtctta ggaatcaatc ggtctgcttc tgccgctgca tttcggatag
     8221 cttgaaaaat tcctaatcta tgatattttg ccaaaggaat ctgacgtatt gctttaatct
     8281 tatcaaggga actatttaaa atatctttaa tataaagacc ttcataatca gctaaagtgg
     8341 caactgccaa ggcatgataa ccttcattat cctcgtcaac atttaataca ggattaccat
     8401 atgcatcttt ttgtccctcg ccaatagtat cactcatatc aagtgcataa atcaattttc
     8461 cagacttgtc aaaataaacg ttcaatccga tataatattc tggcaatggt aaaggcgtct
     8521 ttaataaata aactccttgt accattgtag cttgcgctaa tggtcctttt ccctcgacta
     8581 agtctgtcag caagtctaac tctttttcca agcgcttaac attagctttt ttaatagcgc
     8641 tattattata agctaatacc gctttgttaa tactcctctc aagcaaatga ccattctttg
     8701 ttgccttctt ggtcactaaa gccttctcag ctttagctat ggctttatcc aaatcaacgc
     8761 gaccaaggaa aaattgtgct tcttgaagga atttttgttt ttctccttgt cggaggttag
     8821 attttgataa caacgcttta cctgcagtag tcatttctct aaactgagtt tgagtagtct
     8881 gacgtaatgt agagtaatct aaaccttcta ttatttttaa aaccttttca aggtctacca
     8941 ttgtagaaga tagcttatct ggatcaatga taaaacgctc accatctgct aacatatgag
     9001 aattattatg tgtcctaaca accactttct taccaaagcg tttgtcccca tcattttgat
     9061 caaaagcatt aaaagcgatc tgagataatg tagatgaacc cttcacttct ttcaaatgat
     9121 ttcttttgaa agcctcttca cgaatagtct gtaaatctgg cggtaagact acttcactaa
     9181 gggcattatc cgaaaaagct tggacttcaa ttgtctttaa ttgtttttgc tcagagagat
     9241 ttacactttc cagtttattg gataaaaaag ccatttcacc aattgtttta accttatttc
     9301 cgataaacat aaggtgatgt gcaccatttt gtcgaaaagc tgaacgtcct atttcttgta
     9361 cagattctgg aagaacaatg gcataaatat gattaatatg gaaagcagca tcacctattt
     9421 tgataagttt gtatttcaag tctagagttc caatacgatt attcataaag gctccctctt
     9481 taatctcttc taaatcttca cttgcttcga aggatttcaa gttattagat tgaaaagcaa
     9541 aagcacctat tttccgaata gttgagggga gctttacttc ttccaaatca tatttacgta
     9601 aagttttact ttgaaaatca acattgcgaa aagcgttatc accaatttca gtaatagtaa
     9661 taccattgtg ttgttttgga atttctaagt ttttattacg tcttaccttt tgtaagcctt
     9721 tatttgaaaa acctgtaaca ctattttttt gataggtaaa atcttcctct aaccatttgg
     9781 tataatccat atcaggcgtt gcacgccaca atgatttgtc cggattgacg taagtattct
     9841 cagtcgcaag ttgatgtgga ttttggcctg tccgtgtgcg caatacgacc tgattgttgt
     9901 aatgttcatc tcctggattt cctgtaaaag cttctgattc tattttttca agtccatccg
     9961 gaagcataac attactcaga ttattatctt gaaaacttgc ttctcctata accttaagct
    10021 tacttcccaa aaattcaact gtttgaatac gattagattt gaaagcacgt tctgctaatt
    10081 ttatcaagtg acgtggcaag taaagcttac caccaatctg gttatcaaaa aaagctaatt
    10141 ctccaatgac ctttaggtta tctggtaatt ttacttgttt taaagacatg tgagcaaaag
    10201 catagtctga aatagtctcg agactctcag gaaggttaac ctcagcaata ttcttattgt
    10261 ccacaaaagc atcttgacca atagacttat aaccatttgg aatagtaagc ttagtcaact
    10321 ggtaagcatt aaatatttct ccctcatcaa taatttcctt ctgatcaata tctaaacgac
    10381 tcggtttccc attttctcct agcctacttg tatattctgc aatggccgtc tttttatctg
    10441 gagtaaaagc aaagctagct acttgtgtca attgagttcc atctgctgca tgacttggta
    10501 aaactaagtg tgatgtttga gataacttat taattccaga ttttgaaaaa cctactaaag
    10561 tatcccctct agttacgaaa tcttttgcct cccaagttga tgtttctatg gtttcctttt
    10621 ttgaagctgt atcctgtttt ggtacttcta ctttgctagc atcattacta gaattgcttg
    10681 cttcctgtgt aacccctggt tttgctttgg ggacttgttt atggtcactt accactgact
    10741 cggttttaga actagagcta ttattatttc catcagaaaa taaatcacta actgtttcat
    10801 caacagtaga attctcttta gaaacatttg ttttcctctc attatcttct tcgaaccaat
    10861 catcttctga agctgactgg gtttgttctt gtttgaccga ttcctctctt tctaatccat
    10921 atacctcctg gctgtatgtc actactgata ctgtagtaag tgccaaggca agcgttttaa
    10981 gatgtttttt tgtcattaca ataaagcctt tctaaaagcc aatatatttc tcttaatctt
    11041 ctgattgagt atatttagtt aaatcatgca ggactgacag tcttctaagt ctgatgaagt
    11101 gaaatcatcc agcatatcat catcataact ctcgctacct tctaggatgt cctgtctcac
    11161 tcgtacataa taaatagagg cacatttttg tttaaatgct tgaatatagg ctttatttaa
    11221 atctcgcgta gttgcttgat ctgtcatgaa aagcgttagc gaaatagctt gatcaacgtg
    11281 tttttgagcg gcagctgcaa tattgataat cgcctcaggt cccacttcat aagctccttt
    11341 tttgtagtag acataattat ctgcatcaat cttataagca ggtacataaa ccctccccag
    11401 tgctccttcc ttgcgtactt cgacaggtga tacaactggt tggaggctcg gagtacaaga
    11461 agaaagataa ctgattgacc ctgtcggagc aacagctaat aaatgcgcat tagctaatcc
    11521 tatctcttta atactttgca caagctttct ccaatcctct tggcttggta gagtcaggcc
    11581 atactcagct agtagatttt taacacgctg tgtttttggc tcaaagtttt gttcggtata
    11641 ttgatagaaa taactgccat cagcataact tgattccgag aagccttcaa aggttccttt
    11701 ttcttttgct aaatggttag aagctttaaa ggcataataa gccatagcgt aaaagaagca
    11761 atctgtaaaa tcaatagctt cttgagaatc gtaataaata tggttagtcg ccaaaaatcc
    11821 atgcaaattc atagcaccta aaccaacagc atgattagct gcattacctt ttttaataga
    11881 tggagcacta tctaaatcag aagttcgaga tacacgatca agggctctaa tgctattagc
    11941 aattaatttt tcaaaatccg ccgcttgatc catagcacgc gcaatattaa tagaacctaa
    12001 attacaacaa acatcatgtc caaccttagt gaaagtcagg tcttctgaaa attgactggc
    12061 tgtattaact tgtgcaatct cagaacataa attagacata acaattcgtc cctctttttt
    12121 gtgagggttt ttagcattta cagtatcttc aaataagata taaggataac cagattcaaa
    12181 atgaagctct gctatcgttt gaaatagttt tcgtgcacta ataaaggttt tacgaatatc
    12241 cgcattggcc aataaagttt catattcttc tgtgatagag atatcagaca ttggtttacc
    12301 atatactcgc tcaatgtcat acggtgaaaa caaggccata tctttatttg ctttagccaa
    12361 ttcaaaagta atatcaggaa taacaagtcc tagggagaga gatttaattc gaattttctc
    12421 atcggcattc tcacgtttag tatctaaaaa tgttaacact tcagggtgat gagcatgtaa
    12481 gtaaactgca ccagctcctt gtctttgtcc taattgattg gcgtaagaaa acgaatcttc
    12541 tagtaatttc atgacaggta caataccagt agcttgattt ttaatacctt ttattggagc
    12601 accaaattcc cgtaagttgg ttaagcaaag tgctacccca ccacctcgtt ttgataactg
    12661 aaggcttgta ctaatagcac gagaaataga ttccatattg tcttcaatgc gcaacaaata
    12721 gcaagatata tattcaccac gacgtttttt ccctgcattt aaaaaggtag gtgttgctgg
    12781 ttgaaagcga tttgctagca tgtcatctac tagatcatag gcagcttttt catcaccagc
    12841 agctaaaaac agagcattca ttacaacacg atcttcatag tgttccaaat aataacgatt
    12901 gtcttctgtt ttaagagcat aagcattata aaattttaaa gcccccataa aatttagaaa
    12961 agtaaatttc ttagcgtatg catgctcaaa aatttggcaa ataaactcaa ggttatattg
    13021 ttttagaagg tccgattcat agtagttatt agaaaccaaa taatctagct tttcaattag
    13081 tgatgtgaac gccatcgtat taggttcaac atgttctatc atatattgtt caacagcctc
    13141 tttatccgaa gcaaaatgat aattacctgt gcgatctcta aagcgtgtct tcgcgttcaa
    13201 cgagagatat gcatctgatt gtgtcatctt atttatcctt tctagtgaat tggcaaagta
    13261 actcctttac ccttgtaaca tcttcttgtg ttcccagtaa ttcaaattga tgaagcaaag
    13321 gaacatttaa ttttcgagca ataataggtc cagcaatggc ataagtgtca ccaaaattgg
    13381 tatttccaga agaaataacc ccttggcaat gttcccgatt ttgtcgaaca tttaaaaact
    13441 gtacaacctg ttttggaact gcgcctttgg tatcatctcc tcctccagcg taagtaggca
    13501 caatcaaaat ataatcctca gtaaccaata tactgctacc atctgatgga atacgctggt
    13561 tagaacaacc caacttttga acaaaacgat gggtattgtt agatttcgat gaaaaataga
    13621 ccaccttcac tgtactatcc atttaaaact cccaatcttc atccaatgtt tcttctgtaa
    13681 tccccataat atatgaagag ccgtttcctg agaagaaatc atgattttca tctgcacgcg
    13741 ccgaaagttg tgcaaaaacc tcaggagata tacgagtctc ttcttctgta aatggagaat
    13801 catatcctaa attttgtaaa aatttaccag cattgtaaat gctaaacctg attgcatctt
    13861 cagccaaatc aaaaccatca tatagttcgt aaagataggc tttttctaaa tcaatcaact
    13921 gatagaggag gtcaaagaca aatgtcttca tttcggcttg cttttctaca gataatttag
    13981 caactttctg ttgatactta tagccactgt aatagttatg aattacctta tctcgtaaaa
    14041 ttaaacgaat aatatcagac gtatttggta attttcctct ggctgaaagg taaaaaggaa
    14101 ggtaaaagcc accatataac aaaaatcctg gcatcatggc tgcagcaact ttagatttca
    14161 aaggatcgtc accagtataa aaaggaataa gtatacgtga tcgagcttgt agactttcag
    14221 tatccactac ccattcatgt gcttcctcaa tttgttggct agtgcataag gttgaaaaaa
    14281 tcgttccata cgatcgcgca tgaatagcta ccataaaggc aaagttagca taaataactt
    14341 gttcatgatc tgtctgagaa tgcttaatct gagcaatatc accaactgta gcctgcacag
    14401 aatctaatag ggttagacca gtaaaagtcc gagtaattag ttgctgccaa tcagcatcca
    14461 atgttctcca agaattaagg tcattagaaa ctgggatctt ttcaggtaac cagaaatttt
    14521 gagttactct gttccatacc tctaagtctt tgtcatcgtt tagcttattc caattaacag
    14581 aacgcatagg aaaagctttt tctgacaagg catagtcaag tggcgattga gaacgatcat
    14641 agtatgattg cattaaacta gtctcctaaa tattaaaatt aagcgagttt taagcaccaa
    14701 aaaagcctcc caagaggact ggatttttgt taaaatcaca caactattgg aatgttcttc
    14761 ttgatgtttt ttcattctgg taagaagcta actcttgctt caaaaccact gttttgttaa
    14821 gtcacattaa acttttttgt cggttgcctt tattaaagat ctgcttaaac agtatactaa
    14881 cacaaagttt tgtgtctttc aacatatatc accactatat cttgtgtttt catcataatg
    14941 taaagaatat aaaaattaaa aaattaagcg agcttaattt ttataagaaa aaaaggcttt
    15001 accttcatac ttttcctgtg taaaacaaaa aaggagtagc gactgctact ccttttccaa
    15061 aacccgaaat taatg
//
LOCUS       DATRSD010000054        14675 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRSD010000054 DATRSD010000000
VERSION     DATRSD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14675
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.055"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..778)
                     /locus_tag="VBD85_001363"
     CDS             complement(<1..778)
                     /locus_tag="VBD85_001363"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO7133917.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHK"
     gene            884..1474
                     /locus_tag="VBD85_001364"
     CDS             884..1474
                     /locus_tag="VBD85_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7133918.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            1800..2189
                     /locus_tag="VBD85_001365"
     CDS             1800..2189
                     /locus_tag="VBD85_001365"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO7133919.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            2254..3303
                     /locus_tag="VBD85_001366"
     CDS             2254..3303
                     /locus_tag="VBD85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7133920.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            3448..4983
                     /locus_tag="VBD85_001367"
     CDS             3448..4983
                     /locus_tag="VBD85_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133921.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            4976..6037
                     /locus_tag="VBD85_001368"
     CDS             4976..6037
                     /locus_tag="VBD85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133922.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            6039..6995
                     /locus_tag="VBD85_001369"
     CDS             6039..6995
                     /locus_tag="VBD85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133923.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            7200..8570
                     /locus_tag="VBD85_001370"
     CDS             7200..8570
                     /locus_tag="VBD85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO7133924.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(8691..9680)
                     /locus_tag="VBD85_001371"
     CDS             complement(8691..9680)
                     /locus_tag="VBD85_001371"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7133925.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            9919..12378
                     /gene="gyrA"
                     /locus_tag="VBD85_001372"
     CDS             9919..12378
                     /gene="gyrA"
                     /locus_tag="VBD85_001372"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO7133926.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRAASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAGARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            12385..13128
                     /locus_tag="VBD85_001373"
     CDS             12385..13128
                     /locus_tag="VBD85_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO7133927.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            13144..13557
                     /locus_tag="VBD85_001374"
     CDS             13144..13557
                     /locus_tag="VBD85_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7133928.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            13710..14672
                     /locus_tag="VBD85_001375"
     CDS             13710..14672
                     /locus_tag="VBD85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7133929.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
BASE COUNT         4784 a         2215 c         2990 g         4686 t
ORIGIN      
        1 ctttatgtgg cggtatttgt gtgaatttat gatagtaatt actagggtct gcttcggcta
       61 attgtagaag actgttaaat cgttgtaaat accattcttc aatttgttta ttttcagcat
      121 caatgtatat tgaaaaatca aaataatcat tcatgtaaag tcgatggttt tgttggtttt
      181 gaaagacatt tattccttct aaaattaaaa aatctggtgt ttcaatagtt tgcaattgat
      241 ttggaacgat atcgtagatt tcatgagaat aaattgggat tttcgcggtt ataccgttct
      301 ttatagtatc taagaaattt agaagtgact ccatatcata ggattccggg aaacctttac
      361 ggtttaaaat tccattttgt atgagtttct cgttaggata taaaaagcca tcagtagtga
      421 ctaattcgac gtgactatct ttaaaagttc gtgaaattag taattgtagt aagcgactgg
      481 tagttgattt tccaacagca actgaccctg atatgccgat aataaaagga cgttgataat
      541 tttccttttt aagaaatatt gctttagaaa aagataaatt ttcttgacta cgtttataga
      601 tttgaataag gttaattaaa ggaaggtaga tgtcaataac atcttgaata ttaatattat
      661 cgtttaagct tttgatagat tcaagctctt tttcagttag taaagcttgg gattgctgat
      721 gtaagtcctt ccaattttct ctggaaatac ggtcgaaatt aataaattca ttattcatgt
      781 caaactccta tatgaaagct attataacat tttattgagt gattgaaaac cgtttcaaaa
      841 tatggtaaaa taatatggcg aaattggagg gttagaatta tttatggcta atatgtatta
      901 tacagagaat cctaatgttg aacatgatat tcatgagcta aatgtcaaat tattaggaga
      961 atcattcagt tttttaactg atgcaggagt tttctctaaa agaatgatag attatggtag
     1021 tcaagtcctt ctaaatagtt tacattttga aaaaaataaa tccttgttag accttgggtg
     1081 tggatacggt ccgctgggaa tttctctagc caaagtgcaa ggagttaaag caactatggt
     1141 tgacattaat acgagagcat tggaattagc taagaaaaat gcaacacgca atggagtagt
     1201 agttgaagtc tttcagtcaa acatttatga aaatatttct aaaactttcg actatattat
     1261 aagcaaccca cctattcgtg ctggaaagca agttgttcat tctattatcg aagaaagtat
     1321 ctgttacctt aatacaggcg gtagccttac cattgtcatt caaaagaaac aaggcgctcc
     1381 aagtgcaaaa gctaaaatgc tagatacttt tggtaattgc gatattttaa aaaaagataa
     1441 gggatattat atattgagaa gtgaaaaagt ctaaatgata tccctaaagc gtaaaaataa
     1501 taaatagttc ggataataac tatcgtatta ttttttggaa aggttttctc taaaaatgtt
     1561 ttctttaaat tccgtataag ataaaaagaa ttctgctttt tattctgaaa attataaaaa
     1621 actacttaca ttatctaaaa aaaatggtaa aatagattca gctaaaatac caagactaat
     1681 gaaactacaa gacttggtta ctcaataata aatacttctg tgtttgataa gaagataata
     1741 aacgtgttct aaaaattttg ccagtaaaca ttatgaagcg aaaggtgcag ctactaaaaa
     1801 tgggaaatat agagttgaaa aaattggctg taaaagcgag tgaaaatgca tacgttcctt
     1861 actcgaaatt tccagtaggc gcagcattgc gtacagcaga aggaaaaata tttacaggat
     1921 gtaacgttga aaatatcagt tatggattag caaactgtgc tgagcgtact gcaattttta
     1981 aagctgtttc agaaggttat aaagattttt cggaaattgc tatttatgga aatactgaag
     2041 aaccaatttc tccttgcggt gcatgccgtc aagtgatggt ggaatttttt aataagaatg
     2101 ctaaagttac attgattgct aaaaatggta agacggtcga gacgacggtc ggtgagttgt
     2161 taccatactc gtttgttgat ttaaaataat ttaaatcagt tgctctttta agcatttagc
     2221 ttaaatatta agttttttag gagggttcat ttcatgaaca aaaaaattgc cgggatcggc
     2281 ttggcttcga ttgcagtact tagtttagct gcatgtggac atcgtggtgc ttctaaatct
     2341 ggtggtaaat cagatagctt gaaggttgca atggtaacag ataccggtgg tgttgatgat
     2401 aaatcattta accaatctgg ttgggaaggt atgcaagctt ggggcaagaa gaatggcctt
     2461 aaaaaaggag ctggttttga ctatttccaa tcggcaagtg aatctgatta tgcaactaac
     2521 ttagatacag ctgtgtctag tggttataaa ttgattttcg gtattggatt ttctcttcat
     2581 gatgctattg ataaagcagc agacaataac aaagatgtta attacgtcat cgttgatgat
     2641 gttattaaag ggaaagataa tgttgcaagt gttgtctttg cggataatga atcagcttac
     2701 ttagcaggta ttgcagccgc taaaactacc aaaacaaaaa cagttggctt tgtaggtggt
     2761 atggaatctg aggttattac ccgttttgaa aaaggttttg aagcaggtgt caaatcagtt
     2821 gataaatcaa ttaaaattaa agttgactat gctggttcat tcggtgatgc tgctaagggt
     2881 aagacaattg cagccgcaca atatgcttct ggcgcagata ttgtttatca agtagctggt
     2941 ggaactggtg caggtgtatt cagtgaagca aaatcacgca atgaatcttt aaaagaagct
     3001 gacaaagttt gggtattagg tgtagaccgt gaccaagcag cagaaggtaa gtatacttct
     3061 aaagatggta aggcttctaa ctttgttttg gcttcatcaa ttaaagaagt tggaaaatca
     3121 gttgaattga ttgctactaa aacaagtaaa ggtaaattcc caggtggtaa tgtaacaact
     3181 tatggtttga aggatggtgg agttgatata gcaactacaa acctttcaga tgacgccgtt
     3241 aaagcgatta aagaggctaa agctaaaatt atttcaggag atatcaaagt tcctagcaaa
     3301 taatatcttt aagaactttc aaaaaagcga ttagaaatcg cttttttgaa gctgagataa
     3361 acttatatct cagtaaaatc catcagtcgt taacgattgg tggattttac tgaactataa
     3421 atgaattttt ctgaaaggga gaaccacatg acacagaatg tcattgaaat gaaagaaatc
     3481 actaagaaat tcggtgattt tgtggcaaat gatcatatca atttgacggt tgaaaaaggt
     3541 gagatacatg ctctgcttgg cgaaaacggt gcagggaaat caactttaat gaacatgtta
     3601 gcaggtttac tagaaccaac agacggtcaa atttttatta atggtcagcc tgtaactatt
     3661 gattcacctt ccaaatcgtc acaattgggg attggtatgg tacatcagca tttcatgctt
     3721 gttgaagcat ttactgtagc tgaaaatatt gttctaggaa atgaaaccac ccaaaatggt
     3781 gttttagaca ttaagacagc agctaaagaa ataaaggaac tatcagagaa atatggtttg
     3841 tcagtaaatc caaatgctaa aatatctgat atttctgtag gagcacagca gcgtgttgaa
     3901 attttgaaga cactttatcg cggtgcagat attttaattt ttgatgagcc gacggcggtg
     3961 cttacaccat cagaaataaa agaattgatg acaattatga agagccttgt taaagaaggc
     4021 aaatccatta tactaattac ccataaattg gatgaaattc gggcagttgc tgataaagtt
     4081 actgtgattc gccgtggtaa aagtattgaa acagtccctg ttgcaggcgc aagttctcaa
     4141 caactggcgg aaatgatggt tggacgatct gtttccttca gaactgaaaa aaaggaagct
     4201 aatccgactg acataatcct ttctgtaaag gatttagttg tagaagaaaa tagaggcggc
     4261 gttctagctg ttaaaaatct gtcgttagat gttcgagcag gtgaaattgt aggtatagct
     4321 ggtattgatg gtaatggaca aagtgaatta attcaagcta ttacaggttt aagaaaggta
     4381 acttctggtc aaattgtaat taaaggtaaa gatgtcacaa aattttcaag tcgacaaatt
     4441 acagaattaa gtgtaggtca tgttccagaa gatcgtcata gagatggttt ggttctagat
     4501 atgacgatgg cagaaaattt agctttacaa acttattata aagagccatt gagtcataaa
     4561 ggtatcctta attttgctaa aattaaagag tatgctcgtc aattaatgac agaatttgat
     4621 gtgcgtggtg ctggtgaaca tgttttagca agaggatttt ctggtggtaa ccaacaaaaa
     4681 gcaattattg ctcgtgaagt ggatcgcgat ccagacctac tcattgttag ccaaccgact
     4741 cgtggtctag atgttggtgc tatcgagtat attcataaac gtttgattga agagcgcgat
     4801 aaagggaaag cggtcttagt agttagcttt gagttagatg aaatcttaaa cttatcagat
     4861 cgtattgcag ttattcatga tggtaaaatt caaggaattg ttaaacccga ccaaaccaac
     4921 aagcaagaat tgggtatttt gatggctggt ggaaaaattg aaaaggagga gagagatgtc
     4981 taagaaggcg caaaaaattg cagttcctct gatttcagtt gtactaggaa tcattctagg
     5041 agctattatt atgctgatat ttggttatga tccactttgg ggttatgagg gattgttcca
     5101 aacagcattc ggtagtataa aaaatattgg tgaaattttc cgtgcaatgg gaccattaat
     5161 tttaattgct ctaggtttct cagttgccag tcgtgcaggt ttctttaata ttggacttcc
     5221 agggcaagcc ttatcaggat ggattgccgc aggttggttc gctctttcac acccagatat
     5281 gccacgtcca gcaatgattc tttgtactat tattattggt atcgttgctg ggggaatcac
     5341 tggagctatt ccaggtattc tcagagcata ccttggaaca agtgaagtta ttgtaactat
     5401 tatgatgaac tatattgttc tttattcagg aaatgctatt gtacaacgtg ttttcccaaa
     5461 aagcattatg cgtacaagtg actcgagtgt atatgtttct gccaatgcat cataccagac
     5521 agattggtta tctagtttaa cgaataattc acgtatcaat attggtatct tcattgctat
     5581 tattgcagtt gtgttagttt ggttcctatt aaataaaaca acgcttggat ttgaaattcg
     5641 ttcagttggt ttaaatccaa atgctagtga gtacgctggt atgtctgcaa agcgtacaat
     5701 tattctatca atgattattt ctggagcttt tgcaggactt ggtggtgttg ttgaaggact
     5761 aggtacattt gagaatgtat ttgtacagcc atcctcgtta gctattggct ttgacggtat
     5821 ggcagtaagt ctactcgcag cgaatagtcc aataggtatt ttatttgcag catttctatt
     5881 tggtgttcta agtgttggtg cgcctggtat gaacattgca ggtattccac ctgagttaat
     5941 taaagttgta acagcttcaa tcatcttctt cgtaggtgtc cattacatta ttgagtatgt
     6001 tattaaacct aaaaaacaaa tgaaaggtgg taagtaaatt gagtttaaca acgatttttg
     6061 cattgctatt ttcatcaatg ctaatttacg caacacctct tatctttaca agtattgggg
     6121 gaaccttctc tgaacgtggt ggtatcgtca acgttggttt agaaggaatt atggtaattg
     6181 gagctttctc aggcgttgta tttaatttag agtttgcttc tgtttttggt gatgcgactc
     6241 catggatttc tgtactagtt ggtggtttag ttggtttaat tttttctgtt atccatgcag
     6301 ttgcgacagt taattttcgt gcagatcata ttatcagcgg aaccgttctt aatttaatgg
     6361 caccttcatt agctgttttc ttgattaagg ttctttataa taaaggtcaa actgataata
     6421 ttcaagaatc atttggaaaa tttaatttcc ctatactatc agatattcct tttgttggtg
     6481 atatcttctt taaaggaaca agcttagtcg gttatattgc tattcttttc tcattccttg
     6541 cttggtttat tctatataaa actcgctttg gattaagatt acgctcagtt ggagaacatc
     6601 ctcaagcagc agacacctta ggtattaatg tttatcttat gcgttactct ggtgtcttga
     6661 tttcaggttt ccttggtgga ataggaggag ctgtatatgc tcaatctatt tctgttaact
     6721 ttgcagcgac aactatatta ggaccgggct ttatctcctt agcggcgatg atttttggta
     6781 agtggaatcc aattggtgca atgcttgcta gtctattctt tggtttatcg caaagtttag
     6841 cagttatcgg tagccattta ccacttttga gcaatattcc aacagtttat ctacaaattg
     6901 ctccatatgt cctcactatc attgtattag cagctttctt tggacaagca gtagctccta
     6961 aggcggatgg tattaattat attaaaacga aataaataaa aagaaactag tataagctag
     7021 tttcttttta ttgtttgtga atttgtgaaa aaatactttg tgaaaatttc acataaatta
     7081 taatacaata acaataaaat ataaaaaaat agaaaattga gtcattattc tagtaagagt
     7141 ttgtgaataa tgttataatg tgttttgtta ttaattattt ataaaggaga actcttaata
     7201 tgagtaaaat cgttgtcgta gggacaaacc acgctggtac agcagcaatt aaaacaatgc
     7261 ttagtaatta tggtgaagcc aatgaaattg taacatttga tcaaaattcc aatatctctt
     7321 tcttaggatg tggtatggct ctttggattg gtgaacagat tgatggtcca gaaggattgt
     7381 tctactctga taaagagcag ttggaaagta tgggagctaa ggtttacatg aactctccag
     7441 tacttaatat tgactacgat aaaaaagaag ttacagcttt agtagatgga aaagaacatg
     7501 ttgaaagtta tgaaaaactt attttagcaa ctggttcaca acctatcatt ccaccaatta
     7561 aaggtgttga aattcaagaa ggatctcgtg aatttaaagc tactcttgaa aaccttcaat
     7621 ttgtgaaact ttatcaaaat tcagaagaag ttattgaaaa gttagctaaa cctggtatca
     7681 atcgtgttgc tgttgttggg gctgggtata ttggtgtaga attagcagaa gctttccaac
     7741 gtattggaaa agaagtaaca cttgttgatg ttgctgatac ttgtatgggt ggttactatg
     7801 atcgtgactt tactgatatg atgagtaaaa accttgaaga ccatggtatt cgtcttgctt
     7861 tcggacaagc tgttcaagca gttgaaggtg atggtaaagt agaacgtctt gttactgata
     7921 aagaaacatt tgatgttgat atggtgatct tagctgttgg attccgtcca aatacagaac
     7981 tcggtgcagg taaattagat acattccgta atggcgcttg ggtagttgat aagaagcaag
     8041 aaacaagcgt gaaagatgtt tatgctatag gtgattgtgc gacaatttgg gataattctc
     8101 gtgatgatat caattatatt gctcttgctt caaacgccgt tcgtactggt attgtagcag
     8161 ctcacaatgc atgtggtaca gagcttgaag gtgctggtgt ccaagggtca aatggtatct
     8221 caatctatgg ccttaacatg gtgtcaactg gtttgacttt agaaaaagca aaacaagctg
     8281 ggtacaatgc agttgaaaca ggctttaatg accttcaaaa accagaattt atcaaacaca
     8341 ataatcatga agtagctatt aagattgtct atgataaaga tagccgagtt atccttggtt
     8401 gccaaatggt ctcacacgaa gatgtatcta tgggaattca tatgttctca cttgctattc
     8461 aagaaaaagt tactattgaa aagcttgctt tgaccgatat cttcttctta ccacatttta
     8521 ataagccata taactatatt acaatggctg cattaggcgc taaagattaa tttaaaatta
     8581 aaaaggttaa gcgaaatgct tagcctttta tttcatcctt taacaaggtc aaaaatgtaa
     8641 cggcaaagaa aaagctaaga tattcctatc ctagcttttt ttcaactatg ttagtttttt
     8701 gatgcttctt ggaattctgg gtttttccaa gcttcgtcaa taatatcttt taattgttca
     8761 gctgaggctt gcattttttg aagttcagca tcatttaatg ggatattaac tggacgaacg
     8821 ataccatgtg cacctacgat tgcaggctga ccgataaaga catctttaac atcaccgtat
     8881 tggccttctt gatatacaga taatggaaga actgcatttt catcatcaag gatagcttta
     8941 gtaatacgag caagtgcgac agcaataccg taatatgtag cacctttttt gttgatgatt
     9001 gagtatgcag cgtcacgaac tgaaataaac aaatcaacaa gtccttgttc atcaatatcg
     9061 cgattttctt gtaaccattg ttcaagttgg acaccagcaa cgttagcatg tgaccaaacg
     9121 gcaaattctg agtcaccgtg ttcacccatg atgtatgcgt gaactgaacg agcatcaaca
     9181 ccaattttat ctgctaaagc ttgacggaaa cgtgcagagt caagtgaagt acctgaacca
     9241 ataacgcgtt ccttagggaa acctgagaat ttccaagttg aatatgtaag tacatctaca
     9301 gggttagccg ctactaagaa gataccgttg aaacctgatt caacaacttg agttacgatt
     9361 gatttgttaa ttgctaagtt tttaccaaca aggtcaagtc gagtttcacc tggtttttga
     9421 ggtgcgccag cagtaatgac aacaaggtca gcatctgcgc agtctgcata agtagctgcg
     9481 tagatttttt taggtgatgt aaatgcaagg gcatgtgaaa gatcttcagc atcaccaaca
     9541 gctttatcaa ataaagctgg gatttcaatg atacctaact cttgcgcaat accttggtta
     9601 acaagtgcaa aagcgtaaga agaacctacg gcaccatcac caacgagaat aactttttta
     9661 tgttgtttag ttgcagtcat ttctaaacat ctccttaaat atttttggga agtttcccat
     9721 acacctatat tctatcattt tcaatgaaag aagtcactaa taaaatgtga aaaaatttgc
     9781 aaacaaattg aaatatcata gaaaactatt gaaaacgctg agtgtcttga tatttttgat
     9841 accgatttat catcgcaatt ttacctaaat tatggtacaa tgtaagagac ttacagtgaa
     9901 gaaaaggggc attttttaat gcaagataaa aatttagtag atgttaatct aactagtgaa
     9961 atgaaaacga gttttatcga ttacgccatg agtgtcattg ttgctcgtgc acttccagat
    10021 gttagagatg gtttaaaacc tgttcatcgt cgtattttat atggtatgaa tgaactaggt
    10081 gtgacacctg ataaacctca taagaaatca gcacgtatta ctggtgatgt tatgggtaaa
    10141 taccatccac atggtgattc atctatttac gaagcaatgg tgcgtatggc acaatggtgg
    10201 tcatatcgtc atatgctcgt tgatggtcat ggaaactttg gttcaatgga tggggatggt
    10261 gctgccgcac agcgttatac agaagcacgt atgagtaaaa ttgctcttga gatgcttcgt
    10321 gatatcaata aaaatacagt tgattttcaa gataactatg atggcagtga acgtgaacct
    10381 cttgttttac cagcgcgttt ccctaattta ttagtcaatg gtgcaactgg tattgctgta
    10441 ggtatggcaa caaatattcc accacataat ctaggtgagt ctatagatgc tgtcaaattg
    10501 gttatggata accctgatgt tactacacgg gagttaatgg aagttattcc tggtccagat
    10561 tttcctactg gtgccttggt gatgggacgt tcaggtattc accgtgccta tgaaactggg
    10621 aagggatcaa ttgtcttacg ttcacggact gaaattgaaa caacttcaaa tgggaaagag
    10681 cgtattgttg tcacagagtt tccatatggc gttaataaaa caaaagtaca cgagcatatt
    10741 gtacgtttag cacaagaaaa gcgcattgaa ggtattactg ctgttcgtga tgaatcaagt
    10801 cgtgaaggcg ttcgttttgt tattgaagtt cgtcgtgctg cttcagcaaa tgtgatttta
    10861 aataatcttt tcaaattaac tagcttgcag acaaatttta gcttcaatat gcttgctatt
    10921 gaaaaaggtg ttccaaaaat tttgtcatta cgacaaatca ttgataacta tattgaacac
    10981 caaaaggaag ttattgttcg tcgtactcag tttgacaaag ctaaagcagg agcccgtgct
    11041 catatcttag aaggtttact tgtggcgctt gatcacttgg atgaagttat cacaattatt
    11101 cgtaatagtg agacagacac cattgctcaa gcagagttga tgtcacgttt tgaattatcg
    11161 gagcgtcaga gtcaagctat tttagatatg cgtcttcgtc gtttaactgg attagaacgc
    11221 gataaaatcc aatcagaata caatgactta ctagctttaa ttgctgattt agcagatatc
    11281 ttagctaagc ctgaacgtgt tgttaccatt ataaaagaag agatggatga ggttaagcgt
    11341 aagtatgctg atgcccgtcg tacagaatta atgattggtg aagttttatc acttgaagat
    11401 gaagacctta ttgaggaaga agatgttctt attactctct caaacaaggg atatatcaag
    11461 cgtctcgctc aagatgagtt tagagcacaa aaacgtggag gacgcggtat tcaagggact
    11521 ggagttaata acgatgattt tgttcgtgaa ttggtttcaa caagtactca cgatactgtg
    11581 cttttcttta caaatttagg gcgtgtgtat cgtttgaaag catatgaaat tccagaatat
    11641 ggacgtacag ctaaaggcct ccctatcgtt aaccttttaa aacttgacga aggtgagaca
    11701 attcaaacaa ttattaacgc tagaaaagaa gatgtcgcta ataaatattt ctttttcaca
    11761 actcagcaag gtatagtgaa acgtacaagt gtttctgaat ttagtaacat tcgtcaaaat
    11821 ggacttcgtg ctattaattt gaaagagaat gacgagttga ttaacgtcct cttgattgac
    11881 gaaaatgaag atgttattat cgggacacgt acaggctact ctgttcgctt caaagtgaac
    11941 gctgtacgta atatgggacg cacagcgact ggggtacgag gcgttaatct tcgtgaaggt
    12001 gataaagttg tgggagcttc tcgtatcgtt aatggacaag aagtactaat catcactgaa
    12061 aaaggttatg gcaagagaac agaagcttcg gaatatccta caaaaggccg tggtggtaaa
    12121 ggaataaaaa ccgctaatat tactgctaaa aatgggccat tagcacgctt agtgacgatt
    12181 aatggaaatg aggacatcat ggtcattaca gatacaggtg ttattattcg aaccaatgtt
    12241 gctaatattt ctcaaactgg tcgttctacc atgggtgtta aagtgatgag acttgatcaa
    12301 gaagctaaaa tcgtcactgt tgcgctagtg gaacaagaaa ttgaagataa gtctaatata
    12361 gaggatacaa aagaatagga agttatgcgt aataaaaaaa aatcacatgg tttctttaac
    12421 tttgttagat ggttattggt tgtattgcta attattgttg ggttggcttt ggtgttcaat
    12481 aaaccaattc gcaatgcttt catagctcat caatcaaatc attatcaaat ttcacgagtt
    12541 agtaaaaaaa caattgaaaa aaataagaaa tctaaaacaa gttatgactt ttcatcagtc
    12601 aaatctatta gtacagaatc gattttatct gctcaaacga aatcacataa tttgcctgta
    12661 ataggaggga ttgctatacc agatgtcgaa atcaatctcc caattttcaa aggactgggc
    12721 aatacagagt tgagttatgg tgcaggaacg atgaaagaaa accagattat gggaggccca
    12781 aataactatg ccttagctag tcaccatgtc tttggtttga caggttcttc taagatgctc
    12841 ttttcacctt tagaacatgc taagaaaggt atgaaagttt acctcactga taaaagcaaa
    12901 gtatacactt acactattac tgaaatttca aaggtaacac cagaacatgt tgaggttatt
    12961 gatgatacac ctggcaagag ccaactcact ttagtgacct gtacggatcc agaagccaca
    13021 gaacgtatta ttgtgcatgc tgaattagaa aaaacaggtg aattttcaac agcagatgag
    13081 tcgatattaa aagcattttc gaaaaaatat aatcaaatta atctctagaa ggtagttaga
    13141 attatgaaat taaaggctgt tcatcatatt gcgataattg tttctgacta tgaaaagtca
    13201 aaagacttct atgttaataa gctaggattt gaaattattc gtgaaaacca tcgtccggag
    13261 cgtcatgatt ataaattaga tttacggtgc ggtgatattg agcttgaaat atttggaaat
    13321 cgtcttgatg atccagaata tgaaactcct cctcagagaa taggtaggcc gaattggcca
    13381 cgtgaagcat gtggtttacg acaccttgcc ttttacgttc cagatgtgga agcgtacaag
    13441 gtagaactgg agaatttagg aatttttgta gagcctattc gttacgatga ctatactggc
    13501 aaaaagatga cctttttctt tgatccagat ggtttacctt tagaattaca tgaataataa
    13561 acaaagttgc gtggatacgc aactttgttt aatttactga ataattttat attcaagaag
    13621 ttttttaaag aaataagtaa gttaatcatg gtataatgta aggtatgaaa agaattgaaa
    13681 aggaattatt ttgaaatgag gatactaaaa tgagtattaa aaagctattg tttgcaggcc
    13741 ttgcttttat tttatttacc ctagcgagtc cagcatatgc tgcatcagat gttcagaaag
    13801 ttattgatga aacttatgtt caacctgatt atgtcttagg ttattcgcta aatcaagaac
    13861 agcgtgcaca gacattacaa cttctgaatt atgatgaatc tagagacacg aaagttaaaa
    13921 cgctaaatac tagctcatat gctaaaataa tgaatatagc tgatgatgca agcatccaat
    13981 tatattcatc tgttaaaata aaaaaattag ggtctaacga taccttagca gtaaacattg
    14041 ttacacctga gaatattact aaagttacag aagatatgta tcgcaatgct gcagttacat
    14101 taggtattga gcatgcaacg atatcagtgg ctgctccaat aaaagtaact ggggagagtg
    14161 ctcttgcagg tatctattat tcattagaga aaaatggagc aagtgtatca tcagaaaata
    14221 aacaactagc acaagaagag ttaagtactt tatctggtat taatgctgag aataagggta
    14281 aggaaggtta tgacgccgac aagcttaatg tagctttaac ggatattaag tcagccgtag
    14341 ctaaaggagg atctgatctt tcaaaagatg atattcgaaa aattgttgag gaaacattga
    14401 aaaattatca tcttgataac gctgtaacgg aaaatcaaat aaatttaatt gttaattttg
    14461 cagttaatct ttcacaaagt aatgtcatca aaaatagtga tttcacgaac accttgaata
    14521 atttgaaaga caacattgtt tcgaaagcag gatctaaatt taaaaatatc aatgtcaatt
    14581 ttaatgctaa taaagctgtt gaatcaggta agggattttt agctaatatt tggcaacaaa
    14641 ttgttaattt tttccagcag tttggtagtt aataa
//
LOCUS       DATRSD010000055        14543 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRSD010000055 DATRSD010000000
VERSION     DATRSD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14543
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.056"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..122)
                     /locus_tag="VBD85_001376"
     CDS             complement(<1..122)
                     /locus_tag="VBD85_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEO7133930.1"
                     /translation="MVLRKFQVKAFTVLESLIVLSVVAFMTLVFSTSFNNIFRQV"
     gene            complement(97..426)
                     /locus_tag="VBD85_001377"
     CDS             complement(97..426)
                     /locus_tag="VBD85_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO7133931.1"
                     /translation="MKNLLLKCKDKKVKAFTLLEMLVVLIIISVLLLLFVPNLSKQKE
                     SVTRTGNAAVVKVVESQAELFELQETGRKASLSTLKSGGYITEKQEKAYLDYYKDSSN
                     GSQKISS"
     gene            complement(423..1514)
                     /locus_tag="VBD85_001378"
     CDS             complement(423..1514)
                     /locus_tag="VBD85_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO7133932.1"
                     /translation="MTLRQVILKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RNYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(1399..2331)
                     /gene="tadA"
                     /locus_tag="VBD85_001379"
                     /pseudo
     CDS             complement(1399..2331)
                     /gene="tadA"
                     /locus_tag="VBD85_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(2504..2869)
                     /locus_tag="VBD85_001380"
     CDS             complement(2504..2869)
                     /locus_tag="VBD85_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO7133933.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(2983..6633)
                     /gene="rpoC"
                     /locus_tag="VBD85_001381"
     CDS             complement(2983..6633)
                     /gene="rpoC"
                     /locus_tag="VBD85_001381"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO7133934.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(6750..10325)
                     /gene="rpoB"
                     /locus_tag="VBD85_001382"
     CDS             complement(6750..10325)
                     /gene="rpoB"
                     /locus_tag="VBD85_001382"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7133935.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(10849..13146)
                     /locus_tag="VBD85_001383"
     CDS             complement(10849..13146)
                     /locus_tag="VBD85_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7133936.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIISLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIDTDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            13257..14516
                     /locus_tag="VBD85_001384"
     CDS             13257..14516
                     /locus_tag="VBD85_001384"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO7133937.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGFVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKETVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         4519 a         3030 c         2446 g         4548 t
ORIGIN      
        1 acctgcctaa aaatattatt aaatgatgtt gaaaatacta acgtcataaa tgccactact
       61 gataatacaa taaggctctc caaaacagta aatgctttaa cttgaaattt tctgagaacc
      121 attggaactg tctttataat aatctaaata ggctttttct tgcttttcag taatatatcc
      181 tccagatttt agagtggata aactagcttt tctacctgtt tcttgcagtt caaaaagttc
      241 ggcttgactt tctacaacct tgacaacagc ggcatttcca gttcttgtga cactttcctt
      301 ttgttttgat aaattgggta caaacaataa caacaagacc gaaataatga tcaaaacaac
      361 taacatttct aaaagtgtaa atgctttaac cttcttatcc ttacatttta acaataaatt
      421 tttcatgata atatctccat attttgatac attggtaaca gcattgctgc ataaatcata
      481 acaatgataa gagctacaaa aataaaaata acgggttgga ttaactgggt cgctctagct
      541 aattttgtaa aaaaatcctc ccacttctca tcagcatata tatctaactc tgttcctaat
      601 ttcgccttaa cttggccata ttcaatcatt aaactaagct cagttaagaa aaacggatag
      661 tctaatactt tttggtgaaa tgctttacct gatagaaaac cttcttccat gtcatatcct
      721 atttccctaa aaagtttgga tttctgattt tgcattactt taacaatttg gtccaattca
      781 ataccttgac ttaataaatt tccccactca cgggcatagt aagcagttaa ataaagctta
      841 acatatgatc caactaaggg aattgttgtt aaaaaacaag ctactttaat gcgcgacaat
      901 cgcttttgaa taatataaaa tattaaacta aaaataagta caactgctaa aagtaataag
      961 aaaatattcg gcacatttgt aatcagtcta gttgcaaaat tattttctcc taattggggc
     1021 attaaataat tcctaaggcc aatcataatt agcaccagaa aagacaataa tatcaatggg
     1081 taagtagcta cttctattac tttttttcta actaacaaaa gattagctaa ataagactca
     1141 atctttagta ggctccttga aatgttacca tgcaaatcag ctaatgcaac ctgtgtgata
     1201 acagtgtcag aaaaacctaa ctctcctaac atttttgata aatcttttcc ctctaataat
     1261 gccttattca tactatctgt ataacaatca gacaataact tactcctctt taagaaggtg
     1321 accatatcag ttaaagaaaa accactagca aaaagattat taaaaagttg gactactttc
     1381 cgttgtttct tgagggataa ttttttcgac ttgtgcctgt ttcttactga tatgtccttc
     1441 ttcagccaag atatccactt gtctattcca cttgtctgat gagtgttttt taaaattacc
     1501 tgtctcaaag tcaattaggc ttcctcctcc aattaaacgt tgatatgcta ttaattttag
     1561 actattttct aactcttgat agttaacccc taattctata agcctatcat agactccggg
     1621 aatactttta gcatgaatag tagaaaaaac catcactccc gttaaacttg cacgaataac
     1681 agcacgggcc gtcgcttgat ctctaatctc tccgataatt aaaatatctg gacgatgccg
     1741 taaagacagt ttgattaaag catcataagt cattccaata tcctcattca attggagttg
     1801 taacatcttg tcattcttga tttctaccgg atcttcaatc gtgataattt gcttattttt
     1861 aaatacttct gaagctaatt gatacatgag agttgtttta ccactcccca cagggccgga
     1921 aaaaagatat agccctctta tacccagtac ttccttcatt tgctttatat tatcaaacca
     1981 atattttaag tcctgatgac ctgaatacaa aatacgaata actaaagatt cttgaccacg
     2041 ataatctccc acactcgata gtcgtaatga aaccagtctt ccctctgaca gttcatagtc
     2101 acaagaacct aattgacttc gtcttttttc tccaacgttc atgcctgcca caaatttaaa
     2161 gtgactaata agactagcca tcctattaaa ctcaaaaaca tcaataaacc gcctttcatc
     2221 atcaatacgc atatagagtt cataacaatc acctttggga atgatataaa tatcttgagc
     2281 atttacttct actgcctgat gaatgacttg ctttgctaat gattgaacca tcccgtaacc
     2341 tccttaacca tatattcgaa aataaaataa aaaaatctca ccaattggtg agatcttttc
     2401 aatgatttct tgagaggtag taacataaat attatgaagc agaggctgaa caaacctatt
     2461 atatccccat aaaaactatc taactttttt aaactacttt taattacaaa tttaaagaac
     2521 atgccgatgg caactcagct atcttattaa atacttcaaa tcgctcaata gattcttcct
     2581 taggaatttc ttgccattct ttcaataaca ttaaggaatg atactgttgg aggtactcat
     2641 cacattcttc acaccaacgt ttttctttta tactccaaaa cgttgccaac aaactagact
     2701 tactttgaaa ataaggccac ttttcctgtc ccctatccca ctcctcttga aaatagagca
     2761 aagcttctga taaggtatca tactcagcaa tttcagtaat atcttcctcc caaccttcaa
     2821 taaaccacca tggttcccaa tcaccaaaca tttttacaac ttgatacata atacacttcc
     2881 tttagagagt attatataaa aaataagaat aaaataaaaa atatctgctc attccaaatt
     2941 cttgtaaaaa aagaacctga gttaaactca agttcttttt gattaatctt ctgttgtagg
     3001 tgttgatact tccgtaactt ctgcatcaac aggagttcct tcaataatct caacttcatt
     3061 aacagcaagt ggttcaatat tacggtaacg agccatacca gtacccgctg gaatgatttt
     3121 accgataata acattttctt taagaccaag taagtgatct ttcttaccac ggatagcagc
     3181 atctgtaagg acacgtgttg tttcttggaa ggatgcagct gataagaatg aattagtttc
     3241 aagggaagcc ttagtaatac ccataaggac aggacggctt gtcgcaggga ttccaccaga
     3301 aataacaata tctttgttag catctgtaaa gtctgaaata tccataagtg tacctggaag
     3361 aagatctgta tctcctggat ccatgacacg aactttacgg agcatttgac gaaccattac
     3421 ctcaacgtgt ttatctccga tttctacccc ttggctacgg taaacttttt gtacttcagc
     3481 aagtaggtac gtttcaacag ataaagtatc acgaacttca aggagacgtt ttggttggat
     3541 agatccttca gttaaggccg caccacgagc tacttcatca ccaacttcaa ctttcatacg
     3601 tgctgtaaat ggaacaacgt attctccctc acctgtctga cctttaacga atactttctt
     3661 agtacgtgtt gaagagtctt cttcaattgc aacgacttca cccttaactt cagtaataac
     3721 tgcctcacct ttagggttac gtgcttcaaa gatttcttgg atacgtggga gaccttgtgt
     3781 aatatcagta tttgaagcaa caccaccggt atggaaggta cgcattgtaa gctgtgtccc
     3841 tggttcaccg atagattgag ctgcaatagt accaactgct tcaccaacct caacagcatc
     3901 acctgttgcc aagttaatac cgtaacagtg acgacaaaca ccatggcgag tgttacatgt
     3961 aaatactgat cggatagtta cttcctcaac gcccgcttta acaactttag cagccatatc
     4021 ttcagtaatt aaggtatctg caccaacgag aatttcacct gtctcaggat gtttgattga
     4081 cttcttagta taacgtccta tcaaacgctc ttcaagtgtc tcagtaacct ctttaccatc
     4141 tgtaattgct gtgattgtca aaccgcggtc tgttccacag tcatcttcac gaataataac
     4201 atcttgggca acgtcaacca aacgacgagt aaggtaacct gagtcggctg tcttaagggc
     4261 cgtatcagtc atacctttac gcgcaccgtg agttgagaag aacatttcca aaacactaag
     4321 accttcacgg aagtttgata agattggcaa ttccatgata cgtccgttag gagcagccat
     4381 caaaccacgc ataccggcaa gttgtgagaa gtttgagatg ttaccacgag ctcctgagtc
     4441 catcatcata acgatagggt tctttggatc ttgtgtctca ataagacgtt tttcaagtgc
     4501 ttctttagct tcacgccatg ttgttgtaac agcaacataa cgatcttctt cagtcatcaa
     4561 acctcgacgg aaggctttat tgatatcttc aactctgtgg tgagctgcat caataatctc
     4621 agctttattg tcaataactg ggatatcagc aatacccaca gtcaaaccag caagtgttga
     4681 atgataatat ccaaggtcct tcaaacggtc aaggaaagca gaagtttctg ttgtacggaa
     4741 acgtttaaaa gtttctgcga taatattacc aagatttttc ttcttaaagg gaatattaat
     4801 ctctaagtta tcaataactg cttgaatatc ctgacctggc tctaagaagt atttatctgg
     4861 agttttttct gtcaagtttg cattattagg ttcaattaag tacggtaaat cttcaggcat
     4921 gatatcgttg aagagaatct taccaactgt tgtaaccatg attttatgtt tttgctcttc
     4981 tgtccaaggc ttattcggca ttgaatcaac agcgatacca acacgtgtat gtaaatgaac
     5041 ataaccattt tgatatgcca taactgcttc gtcatgatct ttgaaaatca tgccttcacc
     5101 ttcacgacca gcatcttcca tagtcaggta gtaatttcct aaaaccatgt cctgagatgg
     5161 tgttacgact ggcttaccat ctttagggtt aaggatgtgc tctgcagcaa gcatcaaaag
     5221 acgagcttct gcttgggctt cttcagacaa tggaacgtgg atggccattt ggtctccatc
     5281 aaagtcagca ttgtaggcct cacatacaag tgggtgaaga cgaagcgcct taccatcaat
     5341 aaggacaggt tcaaacgctt ggatacctaa tctgtgaaga gtaggtgcac ggtttagaag
     5401 tactgggtgc tctttaataa cttcttctaa gatgtcccaa atacgctcgt ccccacgttc
     5461 aaccatacgt ttagctgctt taacgttccc tgcgaggtca cgagcaacga tttcacgcat
     5521 tacaaaaggt ttgaagagct caatagccat ttcacgtggt acaccacatt ggtacatttt
     5581 gagtgttgga ccaacagcaa taacggaacg tccagagaag tcaacacgtt taccaagcaa
     5641 gttttggcgg aaacgacctt gtttaccttt aagcatatgg cttaatgatt tcaaaggacg
     5701 actacctggt ccggtaattg gacgaccacg acgaccattg tcgataagcg catcaacagc
     5761 ttcttgtaac atacgttttt cattttgaac gatgatgcca ggagcattta attccaacaa
     5821 acgtgccaaa cgattgttac ggttaataac acggcggtaa aggtcattca agtctgatgc
     5881 cgcaaaacga ccaccatcta attgaaccat cggacgaaga tccggtggga taaccggtaa
     5941 aatgttaaga accatccatt ctggtttatt tccagatttt ttaaaggcat ctaacacatc
     6001 cagacgacga actgctttca cacgtttttg acctgtcgct gatttcaact cctctttaag
     6061 aactgcgatt tctgcatcta aatcaacgcg tttaagaaga tcttgaatgg cttcagcacc
     6121 cattttagca acaaacgaac cgtaaccgta ctcttgaagt ttttcacggt attcacgctc
     6181 agtaaggagt gacttcggtt ctaatggtgt gtccattgga tcgataacaa cataagccgc
     6241 aaaataaata acttcttcaa gtgcacgagg actcatatcc aatgtgagcc ccatacgcga
     6301 tgggatacct ttgaaatacc agatatgaga tactggtgct tttaactcga tatgtcccat
     6361 acgttcgcga cgaacttttg cacgggttac ttcaacgcca caacggtcac aaatgattcc
     6421 tttgtaacga atacgtttgt attttccaca cgcacattcc caatcttttg ttggaccaaa
     6481 aataacttca tcaaaaagtc cttcacgttc tggtttcaat gtgcggtagt tgattgtttc
     6541 aggtttttta acttcaccat aagaccatga acggacctta cttggtgatg ctaatgtgat
     6601 ttgcatactt ttaaaacgat ttacgtcaac cactagtttt acctttcttc gatttatcat
     6661 catactagcc cagctaggat ttcaataaat cctaggacta gcattgcata tcaattctat
     6721 attcgcttct agttataaac tagataaatt taatcttctt gaacgacttc agatacttgt
     6781 tcagcttgtt tttcttcagc ttcttgtttc acacgtgctt tttcaaggtc atcaacgtgc
     6841 atcacatcat catcttcacc ttcatcaaga tcacggagtt caacttcgtt gtcatcttca
     6901 tcaaggacac gcatatcaag accaagtgat tgcaattctt ttacaagaac acggaatgat
     6961 tctggaacac ctggttttgg aattggctta ccttttgtga tagcttcata agctttaaga
     7021 cgtcctgtaa catcatcaga tttgtatgtt aaaatttctt gaagaacatt tgaagcacca
     7081 taagcttcaa gcgcccaaac ctccatctca ccaaaacgtt gtccaccaaa ttgagcttta
     7141 cctccaagtg gttgttgagt aacaagtgag tatggtccta ctgaacgggc atgaagttta
     7201 tcatcaacca tgtggtgaag tttgatcatg tacatgacac caactgacac acggttatcg
     7261 aatggttcac cagtacgacc atcataaaga acagtcttag catctgaatc catacctgct
     7321 tcttgaacag tttcccaaag gtcttctgaa gaagccccat caaagactgg cgttgcaata
     7381 tgaataccta aattacgagc agccatacct aaatgaagct ccataacttg tccgatattc
     7441 atacgtgatg gcaccccaag tgggttcaac atgatatcaa ctggcgttcc atctggtaag
     7501 taaggcatat cttcaacagg aacaatacgt gagacgacac ctttgtttcc gtgacgaccg
     7561 gccatcttat caccgacttt gattttacgt ttttgagcga tataaacgcg gacaagcata
     7621 ttaacacctg attgcaattc atcaccattt gcacgagtaa agattttaac gtcacgaact
     7681 actccatcgc caccgtgagg tacacgtagt gaagtatcac gaacttcacg tgatttatca
     7741 ccaaaaatgg catgcaagag acgttcttca gcagataagt ccttttcacc cttaggtgtt
     7801 accttaccaa caagaatgtc accttctttt acctcagcac caatgcggat aattcccatt
     7861 tcatcgagat cacgtagtga atcttcacca acatttggaa tttcacgagt aatttcttca
     7921 ggcccaagct ttgtatcacg cgtttcagat tcgaattctt ccaagtgaac agatgtatag
     7981 acatcttctt tcacaaggcg ctcactcatg ataacggcat cctcgaagtt ataaccttcc
     8041 caagtcatat aagcaacaac tgggttttgt ccaagagcca tttcaccatt ttccatagat
     8101 ggtccgtcgg cgataaaatc acctttttca acgagatcac caactttaac taaggtacgt
     8161 tggttataag cagtacctga gtttgaacgg cggaattttt gaacatgata aacatcaaga
     8221 gaaccatctt cgcgacgcac ttcaactttt tcagcatctg aaaaaataac acgaccatca
     8281 tgtttagcaa tcacagctgc acctgaatcg tgggctgctt gatactccat accagtacca
     8341 acatatggtg cttttggatc aatcaatggc acagcctgac gttgcatgtt ggcacccatc
     8401 aaggcacggt tagagtcatc attttccaag aaaggaatac atgctgttgc gacagcaact
     8461 acttgcttag gtgacacgtc aacaaagtca acaatgcttg aaggaaactc ttggttatta
     8521 ccttgatgac gacccataac gatttcttct gcaaatgtac cgtcttcgtt aagcttagag
     8581 ttggcttgtg caactgtaaa ttcatcttct tcatcagcag tcaaccaaac aatttcgttc
     8641 gttaccgcac cagtagaacg atctacttta cgatatggtg tttgaataaa tccatattta
     8701 ttgaggtgtc caaatgaaga taagttatta atcaaaccaa tgtttggacc ttcaggtgtt
     8761 tcaatcggac acatgcgccc atagtgggta tagtgcacgt cacgaacttc atatccagca
     8821 cggtcacgtg tcaaaccacc aggtcctaag gcagagagac ggcgtttgtg cgacaattct
     8881 gatagagggt tgtgttggtc catgaattgt gacagctgtg atgaaccaaa gaattcttta
     8941 acggcggcag tcactggacg aatgttaatg atttgttgag gtgtcaagac ttcattgtct
     9001 tgtacagaca tacgttcgcg gacattgcgt tccatacgag caagtccgat acggaattgg
     9061 ttcgcaagta attcaccaac tgcacgaata cgacggttac ctaaatggtc aatgtcatct
     9121 accttaccga taccctctgc aaggtttaag aagtatgaca tctctgctaa aatatctgca
     9181 ggagttaagg cacgaacttt atcttcagga tttgagtttc caacaatagt aacaacacgg
     9241 tctggatcag ttggagcaac aactttgaat ttttgaagaa taactggttc agtaactaca
     9301 gcataatcat ttggtgtgta gacaaacttg tttaagtctc catcgatatg ctctgcaatt
     9361 gaatcaatga catctcgtgt cataacggtt ccagcttcaa caagaatctc tcctgtttca
     9421 ccatcaacta aattttcagc gattgtttgg ttcaataggc gtgttttaag attcagtttt
     9481 ttattaatct tataacgacc aacagccgct aagtcataac gacgtggatc aaagaaacgt
     9541 gcaactaaaa gactacgtga gctgtctgca gtttttggtt cacctggacg aagacgttca
     9601 tagatttctt taagtgcttc atctgtacga gaatcacttg gatttttatg aatgtctttt
     9661 tcaatagtat tgcgaacaag ttcactgtca ccaaagatgt caacaatttc atcgtctcca
     9721 gagaaaccaa gtgcacggac tagtgttgtg aatggaattt tacgtgtgcg gtcaatacgt
     9781 gtataagcaa tatcttttgc gtctgtctca agttccaacc aagctccgcg gttagggata
     9841 acagttgacc cataaccaac ttttccattt ttatctacct tatcattaaa gtaaacacca
     9901 ggagaacgga ctaattgtga aacaatgata cgttcaccac cattgatgat gaaagtaccc
     9961 atttcagtca taattggaaa atcaccaaag aaaacttctt gagttttaat ttcaccagtt
    10021 tctttattaa ctaaacggaa agtaacaaaa ataggtgctg agtagctagc atcatggata
    10081 cgagcttctt ccaaagtata tttaggttct tttaattcat aaccaacaaa ttcaagatcc
    10141 atagtatctg taaagtttga aattggaagt acatcctcaa atacttcttt caagccagca
    10201 tcaagaaaat cttggaatga atcagtttga atttcaatta agtttggtaa atcaagaact
    10261 tccttaattc ttgaaaagct acgacgtgta cggtgttttc cgtactgaac ttcatgtcct
    10321 gccaagtttt tagctccttt ttaaagatag agtgactaag tttttatctc ataaaactta
    10381 gtgagtttat ttcatttgct cgcctaacaa gaacaagctt atagatgtcg tttgcggtaa
    10441 ttttcagaaa gtttaaatat gtattacaat aacctttttt cttaaaatta ggcacaaaaa
    10501 gagcagctaa atctcacttt atcaagttga tttaactgct gtatatctag gtcggacaat
    10561 atttcaacct agacagacga caaatagtta catacattat aacttatttt caccaataaa
    10621 tcaagtttta ttctcttgac ttcaaggaag cttctagaat ttcaatttgt tcatcaacac
    10681 ccttgagttt tagcataaca cgatgatttt taaacatagc atactggtgt ttttctaatt
    10741 ctttgaaaga agactataca agaattaaat catattatct tggaaaaaat aaaaggagaa
    10801 cagatcactc tattctcctc aaataaaact atcagtatcg taaacctgtt atcgttttcc
    10861 acccaaagta gaccaagctt tttgataatc actatcagtt cccccaatag caaaacgata
    10921 ggtcatcgtt ccaggtccat tttttgccca ataacttgtt gtgctttcac ctccaacagt
    10981 gattcgacga ccgtttactg ttactacacc tggctgtaaa cctgtagact taagaacttt
    11041 agcttttata acgctatcat caagtctaaa cctttctgac ttaccaaaag tattgccatc
    11101 tgcattatta atagcattaa ccaagtgagc catataatta gcattattat tgtaacctgt
    11161 caatttcgct aaagaggcat tattatcatg tcctgcccat ccacctaaag tcactttagg
    11221 tgtagaaagc atcaaccaaa catcagaagt tgagttagta gtacccgttt ttccaatcca
    11281 atctacccca gctaaaccac tatttaaacc ttgtaagcga tttttaaagg ttgtagtctt
    11341 tccggagtta attggtccat gtaatagctg ctgtaagata gtagctgttg ctttcgaaaa
    11401 gacacggaca ggtttacttt cgtgattata aatcactttc ccattactat cctctatgct
    11461 ttctatcata tactgcttat gataaacacc accatttgca atcatttgat aaaggtttgt
    11521 ttgctgagca actgacgtat caataccacc accaagtggt aaactttcta taccaaagtt
    11581 ttcaattgga tagtctaatt tctccatata gttttttaca tcaacgcctc gatctcgtag
    11641 catcttgtaa gtccagaaag ctggaatgtt ccaagaaatg tccaatgact cttgaaggtt
    11701 aaccatagca gtaccttctt catccgcatg cataatcttt tcgccacttg aatatgtcgt
    11761 tggataattt gaaagaacac taccactgcc taacatccct tgatcaatag caattccgta
    11821 aggtaatatc ggtttaatac ttgaccctgg agagcgtgcc gtatcaaaag catggttatt
    11881 ttgattctca ctatagttac gaccaccaat aaatcctata atagcaccac tagaattgtc
    11941 cgttagtaca tttcccattt gaaccttgcc agtaccatca tctaacaacc ctccgtattg
    12001 agctgcagca tcctgcatgg cttgataaac agatttattg atagtagttt taatagtata
    12061 ccctccctgt tgaatctctt caatagcgcg gtgtctgtac gtcgctctag tctcatcatt
    12121 ttttaaatca tgctctgaaa cattatcctt tttaatgagg taattgtaca tcactttttg
    12181 agcttcagat aatgccgagt agtaaagata atcatgatga tttgttgtcg ccactgcagg
    12241 tttgataaaa tcttttttga tgtcataatc tttgtaggat ttgtactcat ctttggttaa
    12301 agctctcgtt ctatacatat tgtataacac atttttttgg cgctttatac caaaactaag
    12361 atctttatct gatttcaact gggcatctgc agtataagga gaatatacaa taggactttg
    12421 cggtaaacct gcaaggaaag ctgcctgagg aattgttaaa tcttttgcgg aaactccaaa
    12481 aatcccttgt gctgcttctt caatacctgc aatattctgt cctttgttat tgcgaccaaa
    12541 cggtgataca tttaaataat ctgataaaat cgaatcctta tccatgtaac gctctaatgc
    12601 tagagcatag ataatttctt ttgatttgcg tttaaatgac ggatcatctc ccaaaatttg
    12661 ctgctttaat aattgttgtg tcagcgttga accaccgcta ctctctccaa accccagaac
    12721 agatcctgct gcagcacgta gtacggcttt gggaactaca cctttatggt cattgaaatt
    12781 ttcgtcctct gttgcaatga tagccttttt aatattatca gatatagcat cctttgccac
    12841 tggtgtacgt tgtaaatcag tgtcaatctc tgaaatttgt gatttatcag aataagttaa
    12901 cctagacacc ctagtaagtg tattaacttg tgtcactaaa ctatttttac ttggtacttt
    12961 aacagagtca acttgactag caagataccc tactgcaagt cctactccga gcataacaaa
    13021 tagtgaaata ataacataga tgaaatctga taaaagcttt acaattctta aaaaaataga
    13081 accaaattca agtggtgtgt aatcacctaa tttagaacta ttcaacttct tattaccttt
    13141 aaacatgaat aatacctcat cctacattat atcagattta atataacttg catatcctaa
    13201 agagttttga tatagttgac attgatacac aacaagttaa taattaggag aaaaagatga
    13261 atatttttga tgaactcaaa gaacgtggac tcgtttttca gacaactgac gaagatgctt
    13321 tacgcaaagc tcttgaagaa ggttttgtct cttactatac tggctacgat cctacggctg
    13381 acagccttca tcttggacat ttagttgcta ttttaacctc tcgtcgctta caacttgctg
    13441 gtcacaagcc gtatgcactt gtgggaggcg caactggact tattggggat ccttcattta
    13501 aagatgttga gcgcagttta caaacaaagg aaactgtagt tagttggggt aataaaatcc
    13561 gcggtcaatt atctaatttt ctagagtttg aaacaggtga taacaaagcc gtattggtaa
    13621 ataattatga ttggttcagc aatattagtt tcattgattt cttacgagat gttggtaaat
    13681 attttactgt caattatatg atgagtaaag aatctgttaa aaaacgtatc gaaacaggga
    13741 tttcctatac tgaatttgct tatcaaatta tgcaaggata tgacttttac gaattaaata
    13801 agaactataa tgtcactttg caaattggtg gttctgatca atggggcaac atgactgctg
    13861 gaaccgaact tattagacga aaatcaaatg gtgtatctca tgttatgact gttccattga
    13921 taacagattc tacaggaaaa aaattcggaa aatctgaagg aaatgctgtt tggcttgatg
    13981 ctgacaaaac ctctccttat gaaatgtacc aattttggct caatgttatg gatgctgatg
    14041 ccgttcgctt ccttaagatt tttacattcc taagccttaa agaaatagaa gatatacgta
    14101 ttcaattcga ggaagcacct caccaacgct tagcacaaaa aacactagca cgtgaagttg
    14161 taactcttgt tcatggtgaa aaagcttata aggaggccgt taatatcacg gaacagctat
    14221 ttgctggcaa tataaaagga ttgtctgtta aagagctcaa acaaggttta cgtggagtac
    14281 caaattatca cgttcagacg gaagataacc tcaatatcat agaccttcta gtcacttcag
    14341 gagttgtcaa ttctaaacgt caagctcgag aagatgtctc caatggagct atttatatta
    14401 atggagatcg tattcaagat ttagaatata ctatctcaga aaatgataag cttgaaaatg
    14461 aaattacagt cattcgtcgt ggaaagaaaa aatactttgt attaaacttt aaatagactt
    14521 gaagtgtaat cgatactctg ata
//
LOCUS       DATRSD010000056        14233 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRSD010000056 DATRSD010000000
VERSION     DATRSD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.057"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            154..459
                     /locus_tag="VBD85_001385"
     CDS             154..459
                     /locus_tag="VBD85_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO7133938.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            459..1307
                     /locus_tag="VBD85_001386"
     CDS             459..1307
                     /locus_tag="VBD85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO7133939.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            1304..2026
                     /gene="fabG"
                     /locus_tag="VBD85_001387"
     CDS             1304..2026
                     /gene="fabG"
                     /locus_tag="VBD85_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO7133940.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            2019..2324
                     /locus_tag="VBD85_001388"
     CDS             2019..2324
                     /locus_tag="VBD85_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7133941.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            2308..2784
                     /gene="fabZ"
                     /locus_tag="VBD85_001389"
     CDS             2308..2784
                     /gene="fabZ"
                     /locus_tag="VBD85_001389"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7133942.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            2774..3703
                     /locus_tag="VBD85_001390"
     CDS             2774..3703
                     /locus_tag="VBD85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133943.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            3696..4574
                     /locus_tag="VBD85_001391"
     CDS             3696..4574
                     /locus_tag="VBD85_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133944.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            4571..6574
                     /locus_tag="VBD85_001392"
     CDS             4571..6574
                     /locus_tag="VBD85_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO7133945.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            6571..7524
                     /locus_tag="VBD85_001393"
     CDS             6571..7524
                     /locus_tag="VBD85_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO7133946.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            7521..9716
                     /locus_tag="VBD85_001394"
     CDS             7521..9716
                     /locus_tag="VBD85_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO7133947.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLRAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            9721..10932
                     /locus_tag="VBD85_001395"
     CDS             9721..10932
                     /locus_tag="VBD85_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO7133948.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            10901..11476
                     /locus_tag="VBD85_001396"
     CDS             10901..11476
                     /locus_tag="VBD85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO7133949.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            11820..12161
                     /locus_tag="VBD85_001397"
     CDS             11820..12161
                     /locus_tag="VBD85_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133950.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIYHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            12195..12710
                     /locus_tag="VBD85_001398"
     CDS             12195..12710
                     /locus_tag="VBD85_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133951.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            12762..>14233
                     /locus_tag="VBD85_001399"
     CDS             12762..>14233
                     /locus_tag="VBD85_001399"
                     /inference="COORDINATES: protein motif:HMM:NF017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7133952.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAGAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNS"
BASE COUNT         4934 a         2060 c         2751 g         4488 t
ORIGIN      
        1 tttaaagagt ttcagacgac acagaaagga agaaaacata agaaaggcaa tgcacggctt
       61 gtccatagta gtgtttgaag tagatgttta gaaatatttt attttccaac agtactgata
      121 tcagagcgaa gatgattagt tgaaggtgat aatatgggac gttctaatat tttgagtagg
      181 tgtgaacaat gttctgataa tcaatgtggc ttgtataaaa cgcggcttaa agacaagtta
      241 ctattagtag tccaactggt tggaaatatt aggtattctg agtttcttac ggaaggtggt
      301 gtatatcata agggagaaac cttgttggaa gttataacct taggaatttc ttatgaatat
      361 aaaagttatg gggatatcaa agtaatcaag aagtactgtg ctgatggcag tgtcgttgat
      421 tataacatgc caatactatt aatggaaagg attgaataat gaaaacagta ataatatatg
      481 gaggacaggg taatgttcag tcaaaacacc tttgtcaact agttaaaact ccaaaaggtg
      541 aaattctttt ttcaaagtta caagcaactg atttagaatc atatcagtta atgatggatg
      601 tcattaacca caaggtaagt ttagacaatg ttcatgcgtc tatgttagca aattttttat
      661 ataatgattg gctaacaaac gatgaaaaaa tagtatctgg gataggattt tcagctcata
      721 gcgccggtat tttcaatgtg ttatatgcta gtggtagtac tagttttgaa aacattattc
      781 actttattaa acgtcgtgcc caacttatag aaaattgtca tgtgtctgaa gagctttggc
      841 ttttgatggt tgaaaacatt gatatgttta gtaaggatgt tttaaaagaa cagtctgaga
      901 aagtaaaatt agctattatt actgatgata gagctggtgt aattgctatg gatcaagtca
      961 accgttcttt ctttgatgac gtgatgacta acaaaggtta tttttataaa ttaaagcagt
     1021 taggcgtaaa agcaccatat catactgatt ttttaaatga atctaaagag cagtataaac
     1081 aattgattga aagcctagat atttcgcaaa atgatgacta tgactatatt tttcattgtg
     1141 atcaattggt agatgaaatt ttatatcagt ggaccaatct ctttaactgg cgtgcgatta
     1201 aggaaactat catagaaaag gatgcggaga ttattgatat ttctcctaat aggtttattt
     1261 ctagtcagtt gcaaaaaatg aaaaagaggg ggaggaaaca gttatgaaag tagcaatagt
     1321 tactggtggt accagaggaa ttggaagggc tattacaaaa gagttataca aagaaggtta
     1381 caaagtcatt gctatttata atagcaatga tgctaaggca agagctttac aagaggagtt
     1441 accaaaatta gacgtttata aatgcaatat ttcagatgca aaagcagtac aaaaattggt
     1501 gactaaaatt tttagagaat atggcggtat tgattgttta gttaataatg ctggtattgt
     1561 tagagatggc tttttcttga tgatgtctaa ggaaaaatgg atggatgtca ttaacataaa
     1621 tattatgggg ctagttaaca tgagtaaagc cgtgttaaaa atcatgaagg ctaagcgtat
     1681 acagggaaag gttatcaata tttcttcaac tagtggtata gcagggcaga tagggcaagc
     1741 gaactattca gcaacgaaag gcgctatcat ttccattact aaaactctag cgaaagaatt
     1801 tgcatcagat ggtattacga ttaattgtgt aagtccagga ttcattgaaa cagatatgac
     1861 aaatgaattg caaaataaag aagagctaaa agagcatctt attcctctaa aaaggtttgg
     1921 acaacctgaa gaggtggctt ggctcgtaag ctttttagca agtgagaagg caaactatat
     1981 tacaggaaaa aatattgtga ttgatggagg aatgattaat gattaactgg gctgaagaac
     2041 gtaaaataat aaaaacacgt caagaagtag ttgatgaaat taaaggtatt ttgattgact
     2101 ctttaatgtt ggatattgat aaagatctga ttatgaatga tcaaccatta tttggtcgtg
     2161 gattagagct agactctatt gatgccctag aactgtcaat agggatttca acaacgtttg
     2221 gtgttgaact aaatgatgat gatatttcaa tattatcatc agtaaataga ctagcagatt
     2281 ttgtgattga aaatagtgag gatatagatg accaagctaa gtagtgaaga aattataaaa
     2341 atgttacctc atcgctatcc gtttctaatg gtggatagag tgcagagtta taaaaaaggt
     2401 atcattgaat gtcgtaagaa tattactatc aacgaacctt actttcaagg acattttcca
     2461 caacgtccta tagttccggg tgttttgatg attgagatgg cagctcaatc tgcagcttta
     2521 ctttatattt tagatgcact tgatggtagc ctcccctctc ttgatgaatt aacagcagag
     2581 aaaatagcag aaaaagtagg ttatttagcc agtgtgaaga atttcaaatt caagaaaatt
     2641 gttactccag gagaccagct aaacattaca tgtaaatctc aatcaaaatt ggggcatctt
     2701 ttagaaattc aagtcgttat tagagatgag aataaaaaag aggttgcctc aggaaggatg
     2761 ttggtatcag aaaatggaaa ttaaactcaa aaatattgga aaaaaatatg gttcttttga
     2821 agcattgaga gacattaatc tcatctttga agagggaaaa ttttatggcc ttcttggacc
     2881 taatggagct ggtaaaacaa ctttatttaa ccttttaatt caaaatttta agcaaacgtc
     2941 aggcgatatt aagtgggagg tggatggaaa acctctatct attaaagatt tttatcgtca
     3001 cattggtatt gtcttccaaa gtaatcgctt agatgataat ttaacggttg aggaaaatct
     3061 aatttctaga ggtgctttgt acggtttatc taaatcacaa gtacgtaatc gccttaaaga
     3121 tcttcaaacg tatctagata tcacagcaat aaaaaaacaa aaatatggca gtttatcagg
     3181 aggtcaaaaa cgaaaagttg atattgcacg cgccttgctg ccacaacctt ctctattatt
     3241 attagatgaa ccaacgactg gtttagaccc tcaatctcgt cgcgatttat gggatgctat
     3301 cgcacaacta aatcaacaat cgcagatgac agttgtacta attactcatt atcttgaaga
     3361 aatgtctgct tgtgatgttt taaatgttct tattgaagga aacatttatt attcgggaga
     3421 tattaaaagc tttattgagc aacattccac aacaaacttg aatgtggtat tgaaaccaga
     3481 aaagtcatta gaccaacttt cgatagctga ttttgttaat aaatgtcaag tcctttcaga
     3541 aagggagata gtatttaagg atatttctgt tgaggaaatg atgcaggtta tctcagacaa
     3601 tcaagataaa tctattattg agacatttaa tgttgaatat tcgaacttag aggcggcgta
     3661 tctcaacttg cttagagaaa aagaaaggaa aaacaatgtt tgaactaatt tctagaaatc
     3721 gtaaagttta cacgcgagat cggttagcgt ttttcatgtc ttttttatca gttattatct
     3781 taattttggt ctatcagatt ttcttgggaa aaattcaatt agatgctata aaagcagcgt
     3841 tgaggagtaa tacagtatcc agtgatacta ttcacatggt taattattgg ctcattgcag
     3901 gtttgacgac tattatttca atgactagta cattaggtgc ctttggagtt atggtgtccg
     3961 acaaagaaaa aaaattaaat gaagatttta aggtcagtcc tatatcaaat atcaaaattg
     4021 aattgtccta tgctatattt gccgttttat ttggtattgt catgacgatg ttctcttgca
     4081 tctttgctat cggtattttt aacggcttta attccttgct agagtattca gctatggatt
     4141 atttaactat tttagggatc gtttccttag ggactgtttt atctgcggcg atgattttac
     4201 ctattttagc ctttattcat actagttcag cttttacgac tcttagcaca attgtaggga
     4261 cttttattgg ttttatttca ggagtatatc tctcaattgg ctcggtagga aaagcattac
     4321 aacaggttat gacatggttt ccattgactc aaatcaattc tcttttgaaa caggtcttaa
     4381 tgaagggttc tattgcgaag gtatttgaca aagccaacga agccactgtc tctaactata
     4441 aagaatcata tggtgttgtt ttgcgtaatg ctgatggaga aaggctgagt aatcacttta
     4501 tgttgattta tatcattgcc ctcattctta ttttattggc aattcatttt attattaaaa
     4561 aggttaaaaa atgaaagatg ataataaatt aaagatttct gaagcttcct tagaagatta
     4621 ttctgaagtg gttcatttat ttaataggaa tcatgtttat caatttccag acggtaggcc
     4681 tttaactgtt gatgaccttg atttaacgtt aaaagttaaa gaagtgacac atttattttt
     4741 actgaaaaat catggtgttt tgattggaac atcagctttc tttaaattta ttacctatgg
     4801 ttgtttagat tggaatagta gctttagtgg ttttctttta attgattcga aaagtcgtag
     4861 tggacaggca atcacttatc tatataaaac tatcttaaag aagataacaa aactgaagtt
     4921 ttctaatatc tataccgaaa tcagtaacta caataaaccc tccttagctt tatcaaaatt
     4981 gaatggcttc aaagagtatg ataagacata tgaagacata ctgcattgtc gatcattgcg
     5041 tagtcacctc cctaagatat tgaatacgtt tcgtatttcg aattattatg gtaaaacata
     5101 cgacatatca acttttcaaa ttatggagga aattgaaaat cccttggaag aggagacaga
     5161 aattaggacc aaagtctctg atgaagaaat attatttaaa gcagaagata gtgcttcact
     5221 tccttattat ttaaaaatga gcttgtttca aatggaaatt gctaagttag ataaccgtta
     5281 tgttttacaa gttgactttt tatcggaaca agtaaagagg gttcgggtta aaactggaaa
     5341 atatcacttg gcaaacttaa cgagggctca ccctagccta actttaagtc gttttgccaa
     5401 ttattattac attcaagcaa ctgtggaaac cctttatggt aatatcgatg ttcaactgga
     5461 acgtcgaaaa aagcattata gagatgcaac tatttgtctt aagagaactt ttcaaggtta
     5521 tgatttactt atctctccaa atggcagcct tatttttgaa aagcagaaaa gaaagatact
     5581 tgaagatagt tttttgattt ttagccaacc tttggacaag aaattagttg taaaagaaga
     5641 agaaaatcac attacaatca aatgtttcta tcaaggtgct ttaattgaga aaatcatgac
     5701 atttacaagt gacgaagaaa tcacttgtgt ttataaatgt aatcaaaagg ctaaagagat
     5761 gttcccaaaa ttattgaaac agactttcaa gttacactgc caagagcagt tgattagaga
     5821 tagtgaaggt tatcttgtga atgttccagg aagttacccg attgagcatg atgattttct
     5881 tcgagcagat aagtttgaag atagacaatt ccattattac ctcccgaatg aagataaaat
     5941 gatttcctat tctcctcctg gcaaagccag caatcaaatg caatttagac ccttatgtct
     6001 tattgataca gatagccttt cttttccact gacttatcat tttaggattt cacaagcatc
     6061 atcggaggag gcgttaatca atctaaaaaa acagccaata tgggacagca actaccaagc
     6121 gtcagcaact gaccttttaa aacatatctc taacctaaca ttagaagaag agaaggatta
     6181 tggcatcaag agaatgatag ctaacagaaa gcattatcct agtcacaaac tggttttagc
     6241 ctataatcag attgttttgc ctaagaatga aattccaaga gacagtgaat tatattctat
     6301 ttcttttgat tatcgaatta gaggaaagtt tgtccaaatc cgccaagggg aacatgtcaa
     6361 atatgataat aaatcctatg ttttggaaaa tagtcaacaa ttagtattgt atgtagctag
     6421 cgatgataaa tatatactaa tttctgctaa aaatggaata ttttattctt acaaagagaa
     6481 caatcattta aaaataaggt gcatgtttaa aaggaactca ccttatgcta caaatgtgag
     6541 cattacagaa tatagaaagt gtgaagaaaa atgaatacta tttataatac attgagaaca
     6601 gataaaggtt ataaagttta tgaggggtat ttatatgaaa ttactggtga agaatgtgaa
     6661 gaagccttag accttgtgat tcctaagaat attgtatttg cagatacaga tacttgtggc
     6721 tacacttttt tactcaatga agatggaaca gtttatgatg atgtgacttt ctacaaattt
     6781 gatgataaat attggttggc tagtcataaa gctttggatt cttatttaga caacatcaat
     6841 tttgactata ccgtaacaga tatttctgac gagtataaaa tgctgcaaat tgaaggaaga
     6901 tattcgggag aaattgctca gtcattttat gaatatgata tttcaacact taattttcgt
     6961 actttgatag agatgactta taaaggtgag aaaggttatc ttgctagatt tggtttttct
     7021 ggagaatttg gctatcaatt tttcctacca tcttctattt ttgctacttt tgtttcggat
     7081 gtctgtgaag gtatagcaga gtatggggat gaacttgata gatatttaag gtttgaagtg
     7141 ggacaaccca ttactgatat ttatcaacaa gaagaatatt ctttatatga aataggttat
     7201 tcttggaatc tagatttcac aaaggaagaa tttagaggtc gcgatagctt gttagagcac
     7261 atcagatcag caacagttaa aagtgttgga ttctcaacga aggaaaaact cgcttcagga
     7321 acaccagtgc tatttgatga ccaaattgtt ggaaagattt tttggatagc agacgagaaa
     7381 gactcttcgg aaaattacct aggtttgatg attgttaacc aaacatatgc tcattcagga
     7441 gttacttttg taacagaaga tggccaaatt ttgaaaacac aatcaagccc ttattgtatc
     7501 ccagaaagtt ggaacaaaga atgagcgtat atgttagtgg aataggaatt atttcttctt
     7561 tgggaaagaa ttatagcgag cataaacagc atctcttcga cttaaaagaa ggaatttcta
     7621 aacatttata taaaaatcac gactctattt tagaatctta tacaggaagc ataactagtg
     7681 acccagaggt tcctgagcaa tacaaagatg agacacgtaa ttttaaattt gcttttaccg
     7741 cttttgaaga ggctcttgct tcttcaggtg ttaatttaaa agcttatcat aatattgctg
     7801 tgtgtttagg gacctcactt gggggaaaga gtgctggtca aaatgccttg tatcaatttg
     7861 aagaaggaga gcgtcaagta gatgctagtt tattagaaaa agcatctgtt taccatattg
     7921 ctgatgaatt gatggcttat catgatattg tgggagcttc gtatgttatt tcaaccgcct
     7981 gttctgcaag taataatgcc gtaatattag gaacacaatt acttcaagat ggcgattgtg
     8041 atttagctat ttgtggtggc tgtgatgagt taagtgatat ttctttagca ggcttcacat
     8101 cactaggagc tattaataca gaaatggcat gtcagcccta ttcttctgga aaaggaatca
     8161 atttgggtga gggcgctggt tttgttgttc ttgtcaaaga tcagtcctta gctaaatatg
     8221 gaaaaattat cggtggtctt attacttcag atggttatca tataacagca cctaagccaa
     8281 caggtgaagg ggcggcacag attgcaaagc agctagtgac tcaagcaggt attgactaca
     8341 gtgagattga ctatattaac ggtcacggta caggtactca agctaatgat aaaatggaaa
     8401 aaaatatgta tggtaagttt ttcccgacaa cgacattgat cagcagtacc aaggggcaaa
     8461 cgggtcatac tctaagggct gcaggtatta tcgaattgat taattgttta gcggcaatag
     8521 aggaacagac tgtaccagca actaaaaatg agattgggat agaaggtttt ccagaaaatt
     8581 ttgtctatca tcaaaagaga gaatacccaa taagaaatgc tttaaatttt tcgtttgctt
     8641 ttggtggaaa taatagtggt gtcttattgt catctttaga ttcacctcta gaaacattac
     8701 ctgctagaga aaatcttaaa atggctatct tatcatctgt tgcttccatt tctaagaatg
     8761 aatcactttc tataacctat gaaaaagttg ctagtaattt caacgacttt gaagcattac
     8821 gctttaaagg ggctagacca cccaaaactg tcaacccagc acaatttagg aaaatggatg
     8881 atttttccaa aatggttgcc gtaacaacag ctcaagcact aatagaaagc aatattaatc
     8941 taaaaaaaca agatacttca aaagtaggaa ttgtatttac aacactttct ggaccagttg
     9001 aggttgttga aggtattgaa aagcaaatca caacagaagg atatgcacat gtttctgctt
     9061 cacgattccc gtttacagta atgaatgcag cagctggtat gctttctatc atttttaaaa
     9121 taacaggtcc tttatctgtc atttcgacaa atagtggagc gcttgatggt atacaatatg
     9181 ccaaggaaat gatgcgtaac gataatctag actatgtgat tcttgtttct gctaatcagt
     9241 ggacagacat gagttttatg tggtggcaac aattaaacta tgatagtcaa atgtttgtcg
     9301 gttctgatta ttgttcagca caagtcctct ctcgtcaagc attggataat tctcctataa
     9361 tattaggtag taaacaatta aaatatagcc ataaaacatt cacagatgtg atgactattt
     9421 ttgatgctgc gcttcaaaat ttattatcag acttaggact aaccataaaa gatatcaaag
     9481 gtttcgtttg gaatgagcgg aagaaggcag ttagttcaga ttatgatttc ttagcgaact
     9541 tgtctgagta ttataatatg ccaaaccttg cttctggtca gtttggattt tcatctaatg
     9601 gtgctggtga agaactggac tatactgtta atgaaagtat agaaaagggc tattatttag
     9661 tcctatctta ttcgatcttc ggtggtatct cttttgctat tattgaaaaa aggtgataaa
     9721 atgagtaaat ttttatttgc tcctttcatg atgaatttag gagagagcca aagattatca
     9781 aaactggcta attgtttata tgaacaagga catgatattc atatccttgg agacaattat
     9841 tatccatttt tatttaataa tgatgcttat cgctatcacc actgtgtaga agataaatct
     9901 gtttataata gtaagcgata tgatgctttc ttttctcttt caacagactt taatttttta
     9961 tcagaagaag aaattgaacg catctgttct attgaaagag acttgttacg ccaagaaaaa
    10021 tttgatgctg tattgacagg ttaccgtcta tctattgtta caagttgtcg tttagaaagc
    10081 ataccattaa tttggattat ttcaggggca acgcatatca gtgaaattgt tgaaaactca
    10141 gaaggaatat tgcctaatgg gaagataagt aaagctagta agcctcaaac aaaggatttt
    10201 atcaaaagag taatcactac ttattcaaca aatgttaaaa cgtggaataa ctatattaaa
    10261 aaatatggtg gcaaaccttt taataatgct ttagaattat ttactggtga tctaaacttg
    10321 gtaacagatt actctttgtt ttatgaattt gataaagatt cgtcctataa aacgatagga
    10381 cctattttga tagataatgt aggtttttca aaatgcagcc aaattaatca agacaataag
    10441 actgtactgc tcagctttgg tacttcattt aaacgagatt gggtggaatc ttttttaaag
    10501 acattaccaa ggcattatca ttatttactg acaagctgtg gtgaggctat aaacattcca
    10561 ggaagttata ttgagatagt tgattttatt gattttaaaa ccatagataa agagatttat
    10621 ttcgctatta tacacggtgg gcaaggaaca gtttatgcga tggctgcaca aggtattccc
    10681 tttataggta tccctttctt taacgaacaa ttttggaata ttaagaagtt cagtaaagaa
    10741 aagtgcgctt atttggtaaa gaaacctgat gtaagagaga tacgtaaagc aattagctgt
    10801 cttgaaagag actatgatat ctataaaagt tatatgataa gactaagcca aggaattaaa
    10861 gaagaagcag aggcatcttt acaaaaagcg gaggcagaaa ttgaatcctt tatcagaaaa
    10921 agacaaagtt aactatatta tgatttataa aaataggcag tctacattgg agaataattt
    10981 atctggcgat cggttgatga gatatcaaca agcaaataga caggtaagca aagataaact
    11041 gttaacaggg agttattttg tgtaccaatg cttaaaaaag ttaggtttta ccaataagat
    11101 ttgtcaggaa tctttttcta gtgttagtag ttatttaatt ggtttgccaa aggggaaaat
    11161 tagttactct aattctggtg actatcatat tctaacctat gctcccagtg gttcaactgg
    11221 ggttgatatt gagaaataca aagatagatc agaacaaacc taccaaaact acctaggaga
    11281 atcagttagt agtgatatgg attctaaatt attattttat aaggcatggt tacaaaaaga
    11341 aatttcctat aaatgtggga aatctataga tattacctat caaacaatga tagatggcta
    11401 tatttatggt tatgcttttg ataatacttt taaagtagaa gcggtttact taaaagaatt
    11461 attaccagat aactaactcg caacatgttg ttcctacata tgcatctata aaaagcactt
    11521 tgaagtattt caagggcttt ttataatacc ttgggtagtc tttaacaatt atggttagag
    11581 ttgatatgat aaactgatca tttccacatt ttatgagaaa atcagaaaat agaggtgttt
    11641 ttttgtgata ataattagaa ataataaaaa ctgaaatatt ttctaatgta aaaaaaagat
    11701 aaaagaactt attgctgaaa acgattttaa cttaacattt gccattgaca agtacttaga
    11761 ggagtgctaa aatttcgtta gctggttaac aaaccggtta agtaatgtga ggttctttta
    11821 tgagtgaatt cctttcagcc aaacctattg gtcataatga ttcatctaat actattttaa
    11881 gaattataga gaatacgaaa tatatttatc actatgatgg aaaagttgtt gtcgaaattc
    11941 ctcctcaaaa agagcctatc aaagtctttt ttatggattc acgtcttcat tatttagaat
    12001 tttcagacat atgtcggtat gtaactgttg ttttgaaaaa tagtaccgtt gataatataa
    12061 ccaatttagg gctagataat catattgttt atgacaatgg tgatgatatg gttgatccgt
    12121 tgccgagtat tcagcatgga ttatttctta atgatgatta aagatgtaga caaaaattta
    12181 gaaacgagga agccatgaca ttttataaaa agacggacaa atttggattt aggaaaagta
    12241 aagtgtgccg cagcctatgc ggtgctttat tgggtacagt agcagttgtt agtctagcta
    12301 cagcaagtac agagattcac gctgatgaag caacaacctc acctaccact gtaacgaaag
    12361 tacctcagcc agttcaagca gacacaacag ctctaaatac ctctaagact cacagtacac
    12421 aagcaactac aactcctgtt gaagctaagg agaataaggt tgtcaaaagt gaaactgttc
    12481 aatctgagag ccgagtaatg cctagagata aggtagtaga aaggccagaa acagtaaaag
    12541 ctagtgttaa ctcagacgtt agccaaccta ttacaaccac acccccaaca attaatgaaa
    12601 aaacagttga gattcctaac ttagctcaag atactaagaa ggtagctcca aaagtaactg
    12661 ttacaccaga agttgttaat cagccaaagc agttgcctaa atacgagtaa gaaaactcag
    12721 agaaaggcaa taagagtaca gaagcaacta aggcagttac tgtgactaaa ccacaggggg
    12781 cggttgctga gaaagctact ccggctgtgc caaagcctca aaaagtaaga gtaattgttg
    12841 aatttaataa ggaatctatc ttagattatg cgacagaaca gaaaaaaaca gttgcacagt
    12901 taaatcaagc tgatgttgag aaaaaattac aaagcatcaa gcaagagcaa gataaagttc
    12961 ttaagaatat tgaaaaaagt gttcactttg attcaagcaa agtgaagcgt tacgatgcta
    13021 ttattaatgg tgtcgcactt gatattcaag ctcaagaaat tgaaaaatta aaaacgattg
    13081 ctgatgttag aagggtctat gtctcacaag aatatgttca gacaaaacct ttactatctt
    13141 cctcagggca acttattggt ttgccagaag tttggaacaa ctcccagtat aaaggtgagg
    13201 gaactgttgt tgcagttatt gattcagggg ttgatttcaa acaccaagca ctaaaaatca
    13261 aagaaccaaa tcgtgctaag tataataaaa ctagtattga aaaactgatt catgaaaaga
    13321 atctcaaagg aaaattctat tccgaaaaag taccttatgg ttataactat tatgactata
    13381 atgataacct caaggatagc tacggtgtaa tgcatggtat gcatgtaaca ggtatcgtag
    13441 gagctaatga tgataatcaa aaattgtatg gtgttgcacc aaatgctcaa attttagcga
    13501 tgaaggtatt ttcagatgac caacaaaatc ccacaacctt cacagatgtg tggctaaaag
    13561 ctctagacga tgctatttta ctaaaagctg atgttgttaa tatgagtctt ggaacacctg
    13621 ctggatttgt acatgagggg aaagactatc ctgaacttga agttattgca agagcgtgta
    13681 aggcaggaat cgtcattgca gtagcagcag gtaatgaagg aaatatcact gacggtaata
    13741 cttatggtgt taagccgcta gcagaaaatt atgatacagc cttgattgcc aacccagccc
    13801 tcgatgataa taccttggct gttgcctcta tggagaacct taaaaagcat gctcatgttt
    13861 tgaaatttaa agataaaaaa tctggaacag aagttacaga agtcattaat cttcatgttg
    13921 ccccaaacgc ttcaaaaact attattggat tggctgtaga tttgggagca ggagctcctt
    13981 cggaattgtc aaaacatttt gatttaagtg gtaaaatcgc catgcttgaa attccagaag
    14041 ataataaatc taatggattt ttagaaaaag tacaggcgat tactaagtta aatccagctg
    14101 ctatcttgct ttacaataat gcgaaggtta aagatgattt aggcagtcaa ttattagttg
    14161 aatcagaagc agctaaattt aatattgctc gcattaccag aagcacgtat aacaatatca
    14221 aaaataatag caa
//
LOCUS       DATRSD010000057        14216 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRSD010000057 DATRSD010000000
VERSION     DATRSD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14216
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.058"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            87..1532
                     /locus_tag="VBD85_001400"
     CDS             87..1532
                     /locus_tag="VBD85_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO7133953.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLIHQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(1589..2260)
                     /locus_tag="VBD85_001401"
     CDS             complement(1589..2260)
                     /locus_tag="VBD85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7133954.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(2357..2677)
                     /locus_tag="VBD85_001402"
     CDS             complement(2357..2677)
                     /locus_tag="VBD85_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7133955.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(2686..2814)
                     /locus_tag="VBD85_001403"
     CDS             complement(2686..2814)
                     /locus_tag="VBD85_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7133956.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(2915..3181)
                     /locus_tag="VBD85_001404"
     CDS             complement(2915..3181)
                     /locus_tag="VBD85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133957.1"
                     /translation="MFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENTAF
                     SDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(3266..3709)
                     /locus_tag="VBD85_001405"
     CDS             complement(3266..3709)
                     /locus_tag="VBD85_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7133958.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGG"
     gene            complement(3719..4948)
                     /locus_tag="VBD85_001406"
     CDS             complement(3719..4948)
                     /locus_tag="VBD85_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7133959.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(5072..6133)
                     /locus_tag="VBD85_001407"
     CDS             complement(5072..6133)
                     /locus_tag="VBD85_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133960.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(6168..7187)
                     /locus_tag="VBD85_001408"
     CDS             complement(6168..7187)
                     /locus_tag="VBD85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7133961.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDKDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            complement(7222..8253)
                     /locus_tag="VBD85_001409"
     CDS             complement(7222..8253)
                     /locus_tag="VBD85_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133962.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            complement(8392..9128)
                     /locus_tag="VBD85_001410"
                     /pseudo
     CDS             complement(8392..9128)
                     /locus_tag="VBD85_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
     gene            complement(9149..10234)
                     /locus_tag="VBD85_001411"
     CDS             complement(9149..10234)
                     /locus_tag="VBD85_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO7133963.1"
                     /translation="MQRKSCLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            complement(10418..14216)
                     /locus_tag="VBD85_001412"
                     /pseudo
     CDS             complement(10418..14216)
                     /locus_tag="VBD85_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284822.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         4159 a         2689 c         2257 g         5111 t
ORIGIN      
        1 attagaaaat ctgcaaataa tagctttgtt catcccctaa cttaaatcaa aatttatgct
       61 atgatagtgg taaggaggct tgttctgtga aaaaaacaat tggtattata ggtatgggag
      121 ttagtggttt agctgttcta ctagcacttt ctcgtaaaaa agagaaagag ttaaaacagt
      181 tagctatttt ttgttttgat gatactgtcc actttggacg aggaattcct tttcaagaag
      241 acgttgactc agctttaatc aattccccaa tcaacgatat ttcatttgat tatcgccata
      301 tgtctgactt catggattgg ttagaagaaa attactatga cacaaatcta acctatgtat
      361 caaggacact ttacggacaa tacctttcag aaagaactaa gttactttta gataaactcc
      421 ctgtcacggt tattaaaaca agagtagata aactaaacta tttatcagat gagcaacaat
      481 ggcaagtcta tattcaagat aagctttttc ctgtcacatt tgatgaatta cacttagctt
      541 gtggtgctct gcctgttata gaaccttatc atttggaagg gaagaccgat tatatcaaag
      601 acccttatcc tatcaaatac ttggctcagg aaaattggaa gggaaaaaca gttgctatca
      661 tcgggactgg tctagctgca attgatgtta taaaatggtt gctactcaat acacaatctt
      721 ctgttaaagc attttcaaga accaactact tccctagcgt ccgtattgtt aatgatttac
      781 cacttacttg gcactttttt accaatgata accttaaagc atttctatca catccttcaa
      841 aaaccttcac tattgccgat tttaatgatt tgatgtctgg cgaattggag catcttggct
      901 ttaaaaactg gaaaaataca acagaaactt ttttatcccc aggaattgat ggtctcaagt
      961 tagcctttaa ataccctaag caactctatc tattacaaca attagcttca cgaatcactg
     1021 tctggttaac tgacctttgg ccactcatgt cattatcaga taagaaagag tataaagcac
     1081 tctatggaaa agcgattatt aacttgcgaa atcctatgcc tgatgaatct gctaaagtaa
     1141 ttttgaaagc tgcacaaaaa gggacttttt ctctgatcaa taacgtcaag acaattacaa
     1201 aaaatgacga aggattttca atagaaaaag aaaaacaaaa tcatatatct gtcgatcgcg
     1261 tcattaacgc tactggttac cagttaaaca ctaaaaactg gacaaaagcc aatccacttc
     1321 taaaatcgct catccatcaa gaattttgtc aaattgacga tcaaggaggg attactattt
     1381 taactgaaaa tgcacaagtt ttgtctccaa aatatggtcc aatgccaggt ttatatgcgc
     1441 atggtgcact ggttaatggt gtcgtttatc agaataactc tacaattaaa attcaacaaa
     1501 tggcagagcg agccttttct gataataaat aaccatttct aaaaaatacc tcctctttta
     1561 ttatttaaga aagggaggta tttttttatt ataaattcct ttgttgtaat atagactgtg
     1621 ctatactttc ttttgataat tcttttaagt tatctcctat caattgtaaa gcagtatcag
     1681 ccatatgagc gatctctcta ctatgtgtca ccataataac acatcgtttt tccttatgcg
     1741 caaaatcttt aagacgcatc gtaatatcac gcgcaatgtc aaaatctaaa ttccccgtag
     1801 gttcatcaag taaaatagct ggtgttccta caaccaaagc tctcgcaata gctacacgtt
     1861 gttgttgccc tcctgataat ctcaagatat ttcgcgttaa catatcttct tttaaaccta
     1921 aatccaacaa taattgttta tctgcagtag gttttaccaa ctgaacattt tccaatgggg
     1981 ttagatagtc taaaaggtta taattttgaa agaccaaagc tatattcttt ttgcgatgct
     2041 gagcataacc tgcttcttta atatcttttt tgttaaataa tacttgtccc tcagtaggcg
     2101 aatcaagacc tgccatcaag gatagaaggg ttgttttccc tgaaccagaa ttccctaata
     2161 tagtataaaa tacccctgac tgaaaactat aattgatatt ttctaatgtt ttattgtcgt
     2221 tccccttata acgataagca atatttttta attcgagcat agatttctct ttcttgagct
     2281 atgtattcaa tagcagagca tattcatttt aaccgttaat atagatgtca ctattggtcg
     2341 gtatacttat ggcactttag gacagttttg ataagattcc ccttggtttg tagcgagcaa
     2401 taattaaact agtagtaata atagcaaata agacgattgc taaaccaact agaattacta
     2461 accagacttc ctcaggcttc acttgtacat ttaacgtctt aatcgtttgc ataaacatat
     2521 ctgtatcact atcagcccca agctgcatat tcccaaaccc tttcataaag tctgctctga
     2581 cagttctaga ggcctgatac actaaggagt ctgctaattt ttgaccaata aattgactga
     2641 taagtactga taaagcaaag gctggaatag caatcattag tacttctact gcgtactgac
     2701 aatgataatt gctttagact taccaatagc taataaaata cctgtctcat gggttctacc
     2761 accaatccag aatagcagag ccatcatcac taggataata gatactacta acatgccgcc
     2821 ttgtagagaa gtaattaatt gattcaagac atctatactt tttgcataac ttgttagcaa
     2881 ggttgctgta attgtcagtt ggtatttatt ccaatcaata ggattgtttt ttgataaaac
     2941 ttttaagagt ttctatatca gcattctttt taggatatac agttgtttgg cgataaaagg
     3001 ccttaccttt agaatagccg tataaatcct taatcaattg cgtatctgaa aaggctgtat
     3061 tttctagcat ctcataaggg taatttgatt tttgtaccgt tttaccttta tatatgccaa
     3121 caatagttgc ttcaactaat gttttatcct tactgtttct aacggggtct tttgaaaaca
     3181 ttaacttatc gccaacttta agatgatttg tcttcgcaaa agcctgatga acaattataa
     3241 ctcgctgatc tttcggcgtg atatgtcacc ctcccttaag agaaatcatc cccgctgtgt
     3301 atttaggatc tgtatcggag taactattac catgaacttg taaaacttgt tctttatatt
     3361 gttctttatc atttggtcgt gtaaccattt tttccgaggt tagtccagca taagccatca
     3421 aattattatt aacgcgctta acatttggat tacttgctat gttaactatt gctttttcag
     3481 ggatttctcc agatccgtaa ccatcaccaa caaggtcagt actatccgat gaaatatgca
     3541 aaattgatcc agtttgcctt gaaaatgtct tcttagcagt aagaccagca ttttttaccg
     3601 caataccgct aattaatgta gaagcaacta gccataaaca tacgaataaa gtgatactct
     3661 tacgcttttt acggctaaca tataaaaagg aacgtttaaa taaattcata ttaacctcct
     3721 atgacattcg tgacaatatt tgctttggtt gtagtttaag aatagaataa ctagataata
     3781 caactgcaaa taagcaaatt aaagcaccct gagcatagag tctaaagaca tccattactc
     3841 caacagaaac agcgaggtta tcaattctat caataattag actactattt tgtacaattt
     3901 gtgaactcac accaccatta acttgtgata aaaggccagc tgcaaatgtt tttccaagaa
     3961 agacaccacc aataatagat agtgtgaagg cagcacctgc tactaacagt acttctatca
     4021 gataatgacc tatgatttca aacttagttt ttccgatagc gagtaaaatt cccgtctctt
     4081 taatgcggtt attaatccaa aatgttagca tgacaactaa tattgccatc gccacgagac
     4141 taacaataag aatgattcgt cccactagac gatgcaatgt ctcgatatta cgataagaat
     4201 ctccataaaa ctctttagct gtttgaacat ctaatttccc gaaatcaagc ggccattgtt
     4261 tgatgttttg gataactgta tccactaaac gtttatcact taagcgcact gcaaccgtct
     4321 gataaccttt ttgtttacta atcttcctaa ccaatgttgc ctgagcaatc aatgtattat
     4381 caatattggg attaaaagta tttttagact tagcactatt cgcatcataa attcctgcaa
     4441 tagtaacatt ttctttgcct aatctcaatt tgttacccac acttaaatga ttcttttgtg
     4501 ctaacttcaa tggaactagt acttgttttg tattatcggc cgctaaatgg cttcctttct
     4561 ttaagaccaa tgcttttgtc tgaaaagctc gtaaatcttc tgtactttga taacctgcta
     4621 atgtaacttg attctcttct ttagaagagt tatatggtga tacagatttt aaacggctac
     4681 caacatcaat gcttaaactt gcctctactg actcaaccat ttttgctttt ttcaaagctt
     4741 cgtatgcttg ttcaggccaa taagcctttt ctttggaggt taaataaatg cttttccctt
     4801 gttttgttag gatatttttt tccaaagaat gctgaatact catcagactg attaaacatg
     4861 tgcctatagt tagaaaaact aagaacaagg ttactgtttt cattttttga cgtttcaaat
     4921 aagctaaact taatgtccat aggttcacta ttttccctcc tttttctatc ttaataatct
     4981 ctagaataac gatagaagtc gttagatatt ctctatcgac ttctacgtta tctatcaatt
     5041 agtgatttta tagcactgtt tctaaaaaca attatttctc aaatgttttc ataaattggt
     5101 tagtataatc aattacttct ttaaggttag tatcataaat ttggtgagtt ccgtctagtg
     5161 tatgaataga gacttctttt ggatcttctg ataactcctt atgttgctct tgccaactgc
     5221 ctttaattgg tatatctaaa ctacgtaatt gaagccctaa gtcactttca cgtgctaata
     5281 aatacatagc aggaattccc tttggaatct tatagttggc actttcgtta atcatattag
     5341 aagaattctt cgcttcttct ttcatattag aattattaag tatcttatgt ccaatagcaa
     5401 aaaattgtgc attaatatcg tcggatactt taggaatttt aggtaattct tttaacactg
     5461 gagcagcgaa atcaacttgc ttaccacctt ccatacccgg cgttgatgta tctaaaccta
     5521 ttaatcctag cacttccttt ggataagtac tgatgtattt aagggcataa acaccaccaa
     5581 ttgaatgaga ggcaattaca tattttccgg taataccaac tttttgtaaa gcttcgtgga
     5641 tatctgaggt aatattagct agatttcgtg gttggtcaat gacatcactt aaacctgaac
     5701 caaatggctc aactaccaca accttatgag ttttacttaa attactaatc aaattatggt
     5761 actgatgttt ggcagtcacc gtcccttgac caggaataaa gacaattggt actttacctt
     5821 ctccaacagt atagacattc atcttcttgc ctttgacttc aaccttttga ccatattctt
     5881 tgataggtgt tttgatatat ttttgatatt gtttgttact aataaagctc tctgtgcctt
     5941 tattatattt ttcaagttct ttcgttaact tcattcgctt ggctgctgtt gccttgtctt
     6001 ccttcggatt tgcagttacg ttttcttgct taggatcctc agttttagtt ttttgattat
     6061 ctttattggc agaacatcca acgcaaagca ttaaagtcag ggcgatggtt gataatgtcg
     6121 ttattttatt catcttttct ccttagtaaa ataattgggg ggaataatta tttttcatgc
     6181 gattgaatcc actcgtcaat agccttcgcc atatgtttat attgcgtatg ataaatcaag
     6241 tggctagcat ctagtgtctc aacagaaacg tcttttggat cttcagataa gtcaatatgt
     6301 tgttgttccc agcttttaag cttagggaat atttttttac gcatttttat atcttctgct
     6361 gactcattag ctaagaaata cttagctggc atacctttag ggaacttaac tttttccatt
     6421 ttatgaagat tgtcactaac acgcctatct ttatcgcgct ctgttttatt acctgatttt
     6481 ttagcaccga ttgataagta ttgttcattg gttttcttat caacttctgg aatggattct
     6541 gcccatttta aggttgaagc tgaagattct ccagttgtgc catcaccttc catatttggt
     6601 gtgcttgtat ccatgccaat gaaacctttg acatcgttag aatatgtctt agcataattt
     6661 agaccgtata tgccacctaa cgagtgagcc ataagataga aatttttgac accgactttt
     6721 tcaagagctg tatgaatctc tttggtaata ttttcaatcg tacgtggctt atctgtcata
     6781 tcactaagcc cataaccgag cggttcaacc gtataaattt tatatttttt ccctaatcta
     6841 tcaagcaagt ttttgtaagc ataacgtggt gaaatttcag cttgaccagg gaagactact
     6901 agcggaattt tcccttgacc cattgtatat atgttcattt ttttaccgtc accgacatca
     6961 attttttgtc cgtaatcttc aattggtttc gctttatatt tagttaggtc aatagcacct
     7021 ttagcaatca tagctttttc tgcttctctc tcttgttttt gcttagcgat tgacttttct
     7081 tgcttaggct gctcagtttt agttttttga ttatctttat tggcagaaca tccaacgcaa
     7141 agcatcaaag cgagggcaac ggttgataat ttcgttattt tattcatttt ttctccttga
     7201 cttaggtatt tcattcaacg attacttttg atgggattgg ataaatccat caatagcttt
     7261 agccatatgt tcatattgtg tttggaaaat attgtgacta cctttaagaa tttcaacgga
     7321 agcatctttt ggatcttcag ataaatcaat atgctgctga tcccaatttt tcaaaccttt
     7381 aaaatctgct tgacgtagct tgattccctc cacagattga tcagcaagaa aatacttagt
     7441 tggcatccct ttagggaatt ttacattctt caatttacca agattatcac ctaagcgctc
     7501 atcttcatct cgttgagact tattgccgtt aatttttttc gcaattgaca gatactgttc
     7561 gttagtctta tcatcaacat ctggtaattt cattgcccat ttagcacttt cagggtcata
     7621 ctttgttttt tgttcccctt ccatccaagg tgtactggta tccataccaa taaatcctct
     7681 gacttcttct ggataatttt tagcataatt taaagaatac ataccgccaa gtgagtgagc
     7741 tgcaaggtag aaatttttaa ctccaatttt atttaggcct gtgtgaattt cttttgtaat
     7801 gttttctagc gtacgtggtt tagtaggaat atcactgaga ccatagccta atggttctac
     7861 tgtataaatt ttatattttt tacttaaccg ctcaattaaa ttcttataag catagcgtgg
     7921 cgaaatctca gcttgaccag gaataaaaac aattggaatt tttccctgac cagtttcata
     7981 aatgttcatt ttcttgccat caccaacatc gattttttta ccataatctt tgaccggttt
     8041 tgccttatat tttgtcaaat caacagcgcc ttggggatgc atctcttgat cagcagcttc
     8101 tttgtttttg ggttcttctt tttgcttatc tgtgagttta gtttgtttag gatgatgaga
     8161 ctgtgtttta gatgtagacg tgtttgatgc agcacacgcc gtacaaagta gtaggcttaa
     8221 agcaacagtt gatagctttg ttatattctt catgcattct ccttattgtc tggaaaacat
     8281 agctttacca aagtgtcaaa cgtttttctg gttttagata cattgaatcg ccttttttaa
     8341 caccaaaggc atcataaaat tcttggaaat tacgtactgg gatgttagct ctcaattcat
     8401 atggtgcatg aacatctgac tgaattgagg acttactttg ttctttggtt gctttttgac
     8461 gccaaatact tgcccatgat tcaaaaaatt ctttatagtt tggatttttt cagtcttaag
     8521 agcttctaga gatgccataa caccaccatt atctgcaata ttttctgcta aagttaattt
     8581 accatcaact ttaccgccat ctgctttaag gccatcccat tggtcaatca tagcttgtgt
     8641 ttttttctta taatgtttta aatcttcttt agtccaccaa tagctccata attttgacta
     8701 actgttttag ttttatcgta gagtggtgat tgaaaaatcg ctgctggaaa gactatggaa
     8761 ttggtattag gatcattata agcatttaca gcattggcac tcatttgcca atgttcacgt
     8821 tgattagact ggttaaattc ttcaaatgtt ttcttgttcg ataattttct gtagttatca
     8881 ttgttttcaa agaagcttgc tttactatca aattggtact gacgataaag atcaggataa
     8941 tcttctggat agccaatcat taatctcatg ttatcaagtt tcttaatggc cattgctttt
     9001 gtattttctg ataaccaagt gttgtttttt aaccgtgttt tatagacatt aatgattttc
     9061 ttagccatat gttcgacgtc ttttttagct gcctcaccaa aatatttctt accataataa
     9121 tcacccaaaa tatggccaaa agtatattct aattgggtgt aagcattttt tctttcgatt
     9181 caatctgagt caaaccagac gcaacattct taaaagcact cgctgcttga cgattttgtt
     9241 ctcctagaaa atctgattga tcaactagca tagaaatcat catccatgct ttcatattgg
     9301 ctaattgttt actatttata acatcattaa cctgtttaaa ataagtttta tcttctacat
     9361 tgactgcctt attcgttggc ccaactagtt tttcaatcaa ttctttaaag tcaaattcct
     9421 tggcgtaatt gtgaaccgtt tccatacttt cagggttata tcgccccgca gctgtctcac
     9481 tttcagctgt gattttactt tgatcaactt gcgttttttc tgataagagt ctatcaaatg
     9541 ctatagcttg tttaactagt tttctatctt caatgttact ttttccagct tgtttaagca
     9601 aagccattgc tgaagtacgg taagctgata atttagcctc accttcttta ttgcccttct
     9661 tatattggtc aggtgattca agtaatgcgg gtgcttgacg aagcaccaat tgcttttggc
     9721 tattatctcg agcattggtt tccacagtca aaccaaatgg taaaacaaaa ccactcatca
     9781 caaaatcatg ggccaaactt tgaaagtctt tcattgaaga gactgcttca agtttttgta
     9841 aaactggttt tagaggtttg agaccatttt tatctcttgt tttaaagtcc ataccttgtt
     9901 tatagtatgc aaccattttt ttctgttcat cattggttgt ttcaatcttt cctgaggcca
     9961 ttttagccat atcagacact aacagttgct ttaatttatc ctcaatttct gaaaaactat
    10021 taacactagt ctgcccttgt ttgagttttg tattggctag ccaattatca ttgacagccc
    10081 tgtagagatt ttctctcgga ctgacattat ttgctttctt ctcactggca gatactgcat
    10141 gtaacatagc aatatcttta accacatatc ctgatataag acaagctgtt aaagtaagaa
    10201 cactcatgga ttttaaacag gattttcttt gcataaatga cacctctctt tcttattcac
    10261 ttttagtata gcaagtaggg tgttaaattc ttgttaagaa ttcacttttt tacacttata
    10321 caaaaaagta gtagctaatg agctactact tttgatttaa gagataaaac cttttatcca
    10381 tcacaattaa tgttgctaaa tagattttat tttatcatta atatttgctt ttaaactttt
    10441 tagctaaact aaataataca ctcagcataa cgatagccat tccagtgata taataaccac
    10501 gtttgctgtc accagtggat ggcaatgtat ttttaatttg tttttcaaca tgtgtctgat
    10561 gtgaagttat ctgtttattg tctgttctag ccatatgaac tgcggaaact gtcttagtct
    10621 ttggtaaatc agacgcttta actgtagtat cagtttgatg aacgttatta accttgtagc
    10681 cttcttcaac tataggtgct tcttgggttt tatgtccctc ttcagcttta cgtgcttctt
    10741 cagctttacg tgcttcgtca gctttatgtg cttcttcagc tttacgaata gcttctaagt
    10801 caactacttt aatcggaaca tcaactttat caacaccacc atttacataa tgaatcgata
    10861 ctttagcgtt tgttaacatt cctgattcat ttgtgtttgg agcttcgtcg attgttaatg
    10921 tataagctcc tgccatttct ggaacaacta agtgcttcat caacatatcc acagttactt
    10981 tagctccttt gtcagctgtc atttggcttg ctttaacatc aaaacggttt ctatagatat
    11041 gaagggtctc attacgacga taatttgata agtcaacagc agataactta ataatattat
    11101 ctccaaattc tagtggtact gtcatatctt tagtgatttc tacaccattt ctaaaactat
    11161 aaagaccggt tttatcaaca gattcaagta atgaatcatc ttgatataat tttagtttta
    11221 gagagtcatc tttggcaacg atgtggataa gagcagtatc agaagtcaaa tcacgtttag
    11281 caactgctgt gttaagctct ggaactgatt ggtcaaagaa catgcgacga acaatacttg
    11341 ctttctctcc tgcttctgtt accacttcaa ccctaatcca agtgttaaaa cgactaagtg
    11401 gtaatttagc ccggaactca tattgtttac cggcataaag tactctacgt ccactcatta
    11461 atggttttgt tcgattagtc aagtctttac cttgaataat aacaggggtt cctagcatgt
    11521 ctccattttc attcaccatt ttgacttcaa catgcttgct tttatcacca tccttgatgt
    11581 gaccgataat accagatgct gtgatttcct taccatttag agttgcctca ctagcagttt
    11641 ccaataaggc aggagttaag agaaatactg atgggtattt atgttcacta acaggatcat
    11701 cttgtggtcc agcaactgca aactgtctca tcatcatatt ttcagtaaga gcactcgctc
    11761 ctttaacagc attaaaatta agtttagtat ctgcagtgag attaattcga aggactagac
    11821 tgtcacttgt ttcgtaatac gctgaatctt tttgcatagt aagtagcata tccctcatat
    11881 gcttattttt agataatcta ggacttaaag gaagttgttg aactttctta cttacactac
    11941 ccgtctccat aatcaaactt ttaacattta atgaattttc tgttttatgt aatacaaatg
    12001 ttgtccatcc tttagttaaa ggtaactcac tacctttacc aacttctttt ccattttaac
    12061 ctcattcaaa cgacgaacag ataaactatc aatatgtctg acatcatttg gtaattcaat
    12121 accaatctta acagatttat ctttaagcgc tatacgagaa gtttcttgca atttatcgtt
    12181 aacataaatc ttaccaccct tgtctttaat agcagcaaag cttgaaaata caatggcatg
    12241 gtaatctact gtttcaccag tagctaattg ctcaaaagta gtattgctca aatagtcaac
    12301 cgctgtgata ttgacatatt ttgtatctgc aaaataatct gttgtgatag taatttcttt
    12361 attgcgatta ttattacgat aaattttaag actagtgtca tcaaattgat tagctgacaa
    12421 agcatttcta ggaatgacta atttgccatc agattcgact tgtaatattt tgccatttac
    12481 tttgctagct aaaatagggt catcaacaaa gctaagttga agagacttag catatttaat
    12541 tgctcctgat ttatcttcaa tagcgtcttt tttactatcc tttggtgctt catccggcgg
    12601 cgtgtttaaa tctgggaaac ctggaggagt tgataaacta tctttgggtt tgcttggctt
    12661 agttcccggt ggggttgaat aatcaaaact tctcattgaa gttggtgctg gtaaatcaat
    12721 tcctggtgga cggtctaatt ttggtttttc aactttttta tcttcttttt taaatcctgg
    12781 aggtgtgata aggtctaaat tattattgcc accaccaaca tacttaacag attgaagata
    12841 aacgtctttc aaaccaatgc cagtatcctg aactttgaaa gtaatttttt tccagcgatc
    12901 ttctaacttc tctacagtag tcttatcttt gggacttaag taaggtttat tgttgtccat
    12961 tttgatatag tattgataaa tttggacacc atcgccaccg acacctttat tattcaactg
    13021 tactttaatt tgataaatgt attgtttatc cccttttgca atttaatctt taatcttacc
    13081 atcccaaata gaatctggca tcatggcaaa actattacga taatctaggc gataaagttt
    13141 tctaacgtcg tgtgatctac caagtacacg aacttcttta ttggtatctg gatctataat
    13201 agaatattga acatactcca tattacgcaa agctgcaata cgaactcctg ctttgtcata
    13261 gaaaggacta ttaggagaga aaaatactgt attgttatag attggaagac tcaacatgcc
    13321 gttagttgta aaggttgatg aggtcttgtt ggtttcttta ttaacataga attgaacgcg
    13381 acgttttttg tcaccgttac caatttcaga aatgccttca aaagcatcaa tagctttttg
    13441 ctctgtccat gaaccgtaaa aacctaaaaa tggtaagtgt aaatcggcta caccttcagt
    13501 atcttttaag ttaagataac cttccaagaa attattgcga gttaaagcct ctgcatcaga
    13561 ataatccaca tcaaggtcaa cagctaatgt cttacctgct tctacagtaa ctttccttgt
    13621 aactgcatca gctttttttg atactaggtg ttctgaattt tgcgtacgaa atcctttttc
    13681 atcaactggg tccgcaatcg cttctgatga gatgatatac gttttattag tttttccaaa
    13741 gttacgaagt agaatgcgtg ctttaaattt tttctctttt aattcacata actccaactt
    13801 accatctgca ttattatcgt tagtccctgt tacagttgca acgactgtcg taacaagagc
    13861 tttggcaaga ttcattgctc cgctaccttg ttggcgcaca aagtaaggtg ttttgccatc
    13921 ttttgattgt ttattgataa ttggctgtgc agtgttcatc aagagtaatt taataaaatc
    13981 agcagcatta tcaacaggaa tttttttatc tgtaatgtat tgttttaaaa ctgcagctgc
    14041 cccagctacc tgaggtgcag ccattgatgt tcctgattta tcagcatatt gattatcctc
    14101 cacagttgag aaaatatgac cacctggagc tgtaatctca ggttttagac gtaagtctgg
    14161 tgtaggtccc caagaagaat aagaagataa ttgtcctgct aaactattat caatag
//
LOCUS       DATRSD010000058        13862 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRSD010000058 DATRSD010000000
VERSION     DATRSD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.059"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(488..2083)
                     /gene="cls"
                     /locus_tag="VBD85_001413"
     CDS             complement(488..2083)
                     /gene="cls"
                     /locus_tag="VBD85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7133964.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALTKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2202..3872)
                     /locus_tag="VBD85_001414"
     CDS             complement(2202..3872)
                     /locus_tag="VBD85_001414"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO7133965.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDADTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLLGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAIGLF"
     gene            complement(3961..4980)
                     /locus_tag="VBD85_001415"
     CDS             complement(3961..4980)
                     /locus_tag="VBD85_001415"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7133966.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(5007..5885)
                     /locus_tag="VBD85_001416"
     CDS             complement(5007..5885)
                     /locus_tag="VBD85_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO7133967.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNSEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5854..6672)
                     /locus_tag="VBD85_001417"
     CDS             complement(5854..6672)
                     /locus_tag="VBD85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO7133968.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTEQGRMEAIGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAVRANLKTIVFPCISTGEFGFPNQR
                     AAELAVQAILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6665..6997)
                     /locus_tag="VBD85_001418"
     CDS             complement(6665..6997)
                     /locus_tag="VBD85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO7133969.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(7026..8012)
                     /locus_tag="VBD85_001419"
     CDS             complement(7026..8012)
                     /locus_tag="VBD85_001419"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO7133970.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAERLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLVDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     IAAFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(8009..9208)
                     /locus_tag="VBD85_001420"
     CDS             complement(8009..9208)
                     /locus_tag="VBD85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO7133971.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMVGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVSDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9205..10038)
                     /locus_tag="VBD85_001421"
     CDS             complement(9205..10038)
                     /locus_tag="VBD85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO7133972.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDSQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFEKKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKERSRMISDFYKIGLGDTGSPIAYPNVDSEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGDGEQ
                     R"
     gene            10177..10878
                     /gene="coaB"
                     /locus_tag="VBD85_001422"
     CDS             10177..10878
                     /gene="coaB"
                     /locus_tag="VBD85_001422"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO7133973.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGTLGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKYD"
     gene            10871..11413
                     /gene="coaC"
                     /locus_tag="VBD85_001423"
     CDS             10871..11413
                     /gene="coaC"
                     /locus_tag="VBD85_001423"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO7133974.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATEFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11459..12031
                     /locus_tag="VBD85_001424"
     CDS             11459..12031
                     /locus_tag="VBD85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7133975.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12141..13859
                     /locus_tag="VBD85_001425"
     CDS             12141..13859
                     /locus_tag="VBD85_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7133976.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
BASE COUNT         4469 a         2690 c         2203 g         4500 t
ORIGIN      
        1 atgatagata gtcatactaa cattaggatt tacaatgaga taatagtata tttttaatac
       61 ttcatctaaa ccatcagaaa ctagattatt taaaaatttt acaaagaatg tgatggtagc
      121 atattcaatt aaagttaagt aaagacataa tttttagaag gaaattgtga cttatttatg
      181 caaaaaaaag aaagaaccaa actggttctt tctttttttt gcttttcagc tacttaccac
      241 agtttgtaag gaattacaag tagtagtaag ctaagggcac cttttcaagt gtgataagaa
      301 ggttcacaca acttatcaag atccgctcct gcgttcataa tctccctgcg caatagtggt
      361 aaaaccacat atcaactcat cgcataacag tagtttaaca taatttagtt aaaatgcaac
      421 ctttactttt taaatataac tagattcttc agtttacttt taatattaaa ttaaaaataa
      481 attatttcta aagcagggga gcaatagttt ttgtcactat gccaattact ttttggaatt
      541 ttggaatatt atttatatct ttcaagctaa ctaaccttga tttatttaac gtatccatat
      601 aatctcgata aatgtcttgg attgcatcta ccttataaag atagacagca cattcaaaat
      661 ggtgatacag gctacggtaa tcaagattta tcgtacctac aacagccttt gtattatcac
      721 taaggaatat ttttgaatga acaaatccag gggtgtattc atatatttta acacctgatt
      781 ttgttaatgc ttgatagtaa gttttagcaa gagcataagg gattggctta tctgggatac
      841 ccggcataat aatacgtaca tctacaccac gttccgcagc aaactgaatg gcatgttcca
      901 attcactgtc taatattaaa taaggtgtca tgatgtaaac atattctcta gcatgattaa
      961 gaatatctat ataaacattt tcccctactt tatcagtgtc taaaggagaa tctccataag
     1021 gaataacata gccttcattt tcaactaaat catcgtgtac accaaggtaa ggcgctacta
     1081 acattttttc ctcagtaatg gaccacatct gtaagaaaag taggaggaaa ctatccactg
     1141 ctttcccctt taacataata cctgaatctt tccaatgccc aaataactca atgtgattaa
     1201 tatactcatc ggctaagttt acaccaccag tcatgcctac tactccatca ataacaacaa
     1261 tttttcgatg gtcacgatag ttataataag ttgaaataaa aggagaaatc ggagaaaatg
     1321 cttttgcttt tattcctatt ttctcaagtc gtttcgtata atcaaaggat aattttgtaa
     1381 tttctatcat accatcatat aagatacgga cttcaacacc ttcttccacc ttttgttcta
     1441 aaatactaag tatttctccc cacattattc cttcgtcaat gataaagaat tctaaaaaga
     1501 tatacttttt agcttttagt agttcttctt tcattttttc aaagaatgct tctcccgttg
     1561 gtagaaatgt cacatcagta tttcgataaa ctgggaaatt tccatgtgct ttttcaaaat
     1621 attgaaccaa atgatatgtt gtggatgtac ttgacttaag ctgattcaaa gtttcaggat
     1681 catcctctag ataaatctga ctctcatcaa tcaatgtctc caatctttgt ttcaaaccac
     1741 gataacccca gtcaaatttg gtgtacatta ggaacatcgc ccctagtaaa ggggcaatca
     1801 ttaccaatat taaccaagtt actcgggaca aagcatccat ttcactatta actaaatata
     1861 agaccgctcc aattgttaag atatgctcaa cggttgctaa ccaaacacga tactgctcta
     1921 gccatgagta agaggctagt aaaaacaata attgtaaaat caataaaata gcgataacgg
     1981 ttgttcgact aaatataccc cttagtaaac ttttcttact cttattaagt aaacgaacaa
     2041 ttttgacttt attactgata agtttttccc ccctccaaat caatcatttt attatagcat
     2101 actgtcactt gaatagcatt ctattattct aaattatctc tagttaaaat ctaatcccca
     2161 ataaaaagag gctagcatca caagccctac ctctttatta tttaaaataa tccaatggct
     2221 gtgccgtctt ctaaaacatc catattgaga gctgcaggtt ttttaggtaa accaggcatt
     2281 gtcaatacgt ctccagtaag agcaactata aaaccagctc ctgtttttgg aacaaattca
     2341 cgaacagtta tatcaaagtc agttggagca ccaagtaaat ttggattatc agaaaagcta
     2401 tattgtgtct ttgccataca aataggcatc ttgtcccagc cattgtcact aaactctttt
     2461 aattgtattt gtgccttagg tccaaaatgg actttattac caccatatat tttagtaaca
     2521 atttttttaa ttttttcttc aatagtgtct gcatcattat ataagcgttt gtaattggat
     2581 gattgtgttt caatcacatt agcaacagtc tgtgcaagtt caaggccacc gtctgctcca
     2641 tcttcccaca cacttgctaa ttcaactgcc acatcaatat tactacataa ggttttaaga
     2701 gttgcaattt cactttctgt atctgcagta aactgattga tagctacaac taccggaaca
     2761 ccatattgac gcatattgtt aacgtgacgc tcgagatttg taaaacctct tttaactgct
     2821 tcgacgttct cttgtgagag atcttctttt gaaacacctc catgcatttt caatgcacgc
     2881 aatgtagcaa caataacaat cgcatcggga gaagtcggaa ggtttggcgt ttttatatca
     2941 agaaactttt cagcgcccaa gtctgctcca aaaccagctt cagtaacaac gtaatctgct
     3001 aagcgtagag ctgttgaagt cgctaagaca gaattacatc catgtgcaat attagcaaat
     3061 ggtcccccat ggacaagagc cggtgtccca taaatggttt gaaccaaatt aggtttgata
     3121 gcatctttta ggataagtgt gagagctcct tcaatcttca aatctttaac ataaattggt
     3181 ttacggtccc ttgagtaagc cacaacaata tttgacaggc gtttctttaa atctgatagg
     3241 tcagtagcta aacatagaat agccattatt tcagaagcaa cagtaatatc aaagccatct
     3301 tcacgtggta tcccattgac agggcttcct aagccaacga tcacctgacg aagagcacga
     3361 tcattaagat caactacacg cttccaaatc actcgacgtt ggtcaatatc taattcattg
     3421 ccttgatgaa tatgattatc aagtagagct gataaagcat tattagcagt tgtgattgcg
     3481 tgcatatcac cagtaaaatg caaattaata tcttccattg gtagtacttg tgcgtatcca
     3541 cctccagcag ctcctccttt aatacccatt acaggaccta aagaaggttc gcgaagcgca
     3601 atcatcgttt ttttaccaat tttatttaaa gcatcagcta atccaatgct catagtggat
     3661 tttccctcac ctgctggtgt cggattaata gcagttacta aaattaactt tcctactttt
     3721 tgagatctta cagcttcaat cttatcaaaa gataattttg ctttgtattt accatacaat
     3781 tctatatcat caaaaccaat tcctacttgc tcaacaatct cagcaatagg ctttagggcg
     3841 acgctttgcg ctatttcaat atctgttttc atgatattct ccttctaaat aataaaaata
     3901 gtatagcaat attccctata aaaacatatt tttttgcaaa aaaaatttaa aatgttcgtc
     3961 ttatgaaaat aaaactgctt ttatttcttc tggactagtc tgaccaaaat aatcttctaa
     4021 tgataaagta cttaattttt ccaagactaa ttcttctttc atagggcaac caatgagaag
     4081 gttttcaata tctgaaatat ctttagaact gaaaaagtca ccataaatac ggcaattcgt
     4141 aataatacct ttctcaattt ctaagtgaaa atcgtaagtt cctgacggaa aacgagcatc
     4201 tctgtgatac gaatactgtg gtgagttacc ataattccaa tcccaattct tatatttttc
     4261 atcagctaga gcgtcaatcg cagcccaatc tttttcggtt aggtgatagc gttttgcctg
     4321 atcaatatga tcaatatgta ataattgaca aaccattaaa tctttaaatt gatcggttgt
     4381 gatatgacga taatcttctg acaagtggct acgaatatct cctactcgac ttcggacaga
     4441 ttttatccct ttagattcaa tttttttccg attaggattt aacacttttt ccatagcatc
     4501 aaaatcaaca tctaataaaa gagagtaacc accgtaaata cgaccgttta ctatcgtcat
     4561 tgctgcacct gatacttttt taccatcaat aactaagtca tttcgtcccc tcatttcaac
     4621 actagaggca cctaaagttt ttagggcctt aatagctggc tcataagctc gtttgaaatc
     4681 accaaattgt ccatgatctt tcatgagata gcaaatattt acagcacctg agtcaacgta
     4741 aacagcccct ccaccagtat ctcgacgaac aacaggaata tcatgttctt tcaaatattc
     4801 ttgatttgtc tctataactg cattctgaaa ttttccgatt tcaactttag gatcacaata
     4861 gtatggaaat aaaatatcat catctaaaaa aacattttgt aaaatataaa cttgcatggc
     4921 aagtgcaatc gcaccgtctg tgatacgctt accatttcta attggttcaa taagatacat
     4981 ttaggtctcc ttttatagca atttagttat ccaataattt cttttttcgc ttcagcaatt
     5041 aattcagcga tatgctgtgt cagttgcaca ctccttgagc gaatcttttg aggaatgcga
     5101 taatgcttgt gattaagagt aacaaataaa gagttttcat ttaaagagac gaatcgccaa
     5161 aacggatgct taataaattg tggcgtggta tgtccaactc caatctctag ataaagaact
     5221 ttgtcatttt gatgttgtga taagaaattt tcataacgtt gtcgttgagc atgaaagtca
     5281 gcatcttcca ccataccttt ttcttcgtta cgtttattaa tttcaaacgg ttgatcacac
     5341 tttgggcatt tggggattaa gttactagga atcttcatat ccttttgttg tgcaatcatt
     5401 tgacgaattg cctgatcatt tcgataagtt tgttgatgac aatgttgact acactgccat
     5461 aagccatatt caccttgaat atgaaaaact ttttctgaat tataatcagc cacagcgaag
     5521 gcattatcag cattagttgt aataatatga tattcttttt tcgctaaaat atcctttaaa
     5581 tcaagataag cttgccctac cggttgatct aaataattaa gtgccacaaa acgactttgg
     5641 aaggcccaat attcttccca atcttcaaag tcataaagac ttgcttgcaa catatctaat
     5701 agttgatatt tagcaataaa atccggaaaa gcttcttcaa atctggggcc aatataggtg
     5761 aatccatccg ctgcagacat gccagcacca ataccaacta caatagcatc tgattcttct
     5821 attaactgac ttagtatttc tgattgagaa tgattagttt tttctagagt cttccacact
     5881 gtcatctatt tcctctttta ataataattt ttgataaatc tcttggtctt taggtgtgaa
     5941 tgtattgaaa ataatataga gtttgtgctg attttcgcgt tgccactcta atatcgcttg
     6001 aacagccaat tcagcagctc tttgattagg aaaaccaaat tctcccgttg atatgcaagg
     6061 gaagacaatt gttttaagat tagctcgaac tgccaactgt aagcaggatt tataagaaga
     6121 ctttaataaa tcctctctta tacgagatgg cttttgatca acttttacat aaggtccgac
     6181 tgtatgaata acatacttac aaggaagatg ataggattcg gtaagtttag cttgtcctat
     6241 ggcttccatt cgtccttgtt ctgtcatcaa ttgatgacat gctaaacgta atcgtgaacc
     6301 tgcaaacgta tgaatctgat tgtcaataca atggtgatta ggaataaaac aacctaacaa
     6361 cttactattt gctgcattaa caatagcatc cacttgtaat aagcaaatat ccccatggta
     6421 ggtaaataca tttgaatata ccgtttgatg acagacgtct atcgttttta ctttttgagt
     6481 attccaccaa tgtgacaaat acctatcttc taaagacagg tactcttgac taatgttttg
     6541 aggatcttgt tgattaacta gaccacgcca aaccgtttgc aaatcggtgg tcctcaagtc
     6601 atcaacgtct gttaattttt ctgactgaag atactcaatc atggctagca atagttgttt
     6661 ttgattaggc attttctaaa gcttcaaaag cagcataatc aacgtctgtt aacacaacca
     6721 accaattttc gtcagctttt tcagaattta acaaagttgg ttcttgactt gcagctgtat
     6781 taactttaac aactttacct gtaagaggag ataaaattgc cataaccgtc tttgaagctt
     6841 caatatttaa aataacatca tcaacttcta aatttgtatc gtctgtgaat tccacatatc
     6901 caacagttcc caagtcatct tgaagttcgg gagttaagct gatagtataa agttcttcat
     6961 ttttttcaat taataagtag ttcgcaattt ttttcatgag tattttcctt tctgagaaaa
     7021 cgtgattaat ataactgtga taataatttt aaggatttga ttcgttgatc aagccctgga
     7081 acaagaggaa tcaaaagtag ttcttctgct tgactagcat taactaaagc gtcaaggctt
     7141 tctttaactt gttttggatc acctacaatc atacggtggc gatttgattt aatttttgct
     7201 ttttgttcag gagtcaattg gtaatgatta gcttcttcaa tagtaggaaa tgttgcaaat
     7261 tcattgaagt ctttatttcc tagcatccag atatctaaag tctttgctat gttttcagct
     7321 tcttctgaag tatcagctat aactacaaat gcagctatca tcaaattagg agacttatta
     7381 ggataatatt cttcaaactg atgataataa agagatgaaa gctttttagc ttctgtcaat
     7441 gggtctttgt ccataaaagg aaaaacaccg aaggtaaagc ctaagccaag tttagccgct
     7501 aaataagctg atttttgacc actccccaac acatataagt ctgggaagct agaaagagtc
     7561 ggttgaacta agggttcctt actggatgat tcatcaacaa gccatgactt caattcctcc
     7621 agtacctctt cgtagtcatg tgctttatgt aagctacgaa gtgcatttga aactttaact
     7681 gtccctagtg aatttcctaa accaatactt actcgattcg gatgacatgt ctctagtgtc
     7741 ttgagagtct ccgcgagttt aaaactacta tagtgcagag gcattatacc tccagagcca
     7801 attttgatag atttagtctg gttagccaaa tgcattatca ttaattctgg attgctaata
     7861 ctgaatgcct taacaccgtg atgttcagcc acccaaaatt gatgatatcc tagtcgctcc
     7921 gcctcttgtg ataaaagaat cgtttcttgt attgcatcac ttgcagtttt gccataatca
     7981 ataagcccat aatcaaggac acttaatttc atctttcctc cctataattg cttcgaagtt
     8041 ctctaaaaac ttctcgagct tcctttggta aagattctcc ataatccatc aaaggaacaa
     8101 tatctttaaa ggaggctttt ggaatcgcca aaccttctaa atcactcact ttaatcctta
     8161 aattaatctg atcagaacgc ttagctttta ttttttgtaa aaattccgga tcaactacta
     8221 agggcgtgga agctcctacc atatgagcat gttgtaaggc ttcaaacacc tttgaagcac
     8281 tattaatacc tccagttgcc ataattggaa ctctatcacc aaaatgttca aatataactt
     8341 gattaaccag ctgccctttg taaactccct cagaacgtat agtatttcta aagacatcat
     8401 gcccccagct tgcaatggca agataatcta cctgagcaat cgctaaaatt ttttcaagaa
     8461 attccataaa ctcttcaatg ctatatccaa tttgtgaacc gcgagtttct tcaggcgtcg
     8521 ctctaaaacc aaggataaaa tctgactctg cttcttctct gataactttt tgtaccgctt
     8581 taaaaacttc taagcctaaa cgacagcgat tagtcaaagt ttgaggacca tactcatctt
     8641 ttctttgatt tgagaaggtt gagaaaaaag tttgaattag taatcgttgt gcagaagata
     8701 tttcaacacc atcaaatcca gcttgtatag ctcgtcttgt agcctggaca tattcatcga
     8761 taattcttag tatatctttg tgtgttagtt ctttaacctg atgtgggtat ggcgattgta
     8821 attgcatagg gctgggacca tatacatatc cgtgtcttgc caaagtgtga cttgagaaac
     8881 gtccagcatg cgtcagctgc aaaacagctt tcgctccctt agacttcatt gctttagcca
     8941 atttagtcaa accaggaatg tcctcatcct ttgaaacgct aaaaccatat tcaaataact
     9001 gaccatattc tgtgatatat gctgctccag taatttgtaa tggagcggat ttagcacgac
     9061 gaacagcata tgctatgtcg tcatcagtta caaaaccttc tgatgtagaa gaattagtta
     9121 ccataggtga tagaacaaaa cgattttcta agctcaatcc attgggaagc gttaatggac
     9181 gaaataaatt accttggaca ttattcatct ctgctcacca tcccctttat tagccataga
     9241 cttagcctga aatctatcaa actctgcaac taaatcgggt ctaatcatca gtaaacgatc
     9301 attaaaacct acttgtttaa ggctaatcaa catacgatca ataacatctt ctactgtcat
     9361 aggacaatct aatagatcag atagattagc cataatttca gaatctacat ttggataagc
     9421 aattggacta cccgtatcac ctagaccaat cttatagaaa tctgaaatca ttcgactccg
     9481 ttctttttga tcgccacaaa cgctaaggta aatcgatacc gcaataccat tctttatacg
     9541 gcgctgagcc aagccagcaa attttttgcc atttatacta agatcaaact taccaggaca
     9601 ataggaggtc tctacttcaa agtgttcaat aggttgataa aaatccgaaa atatacttct
     9661 aataaaatcg accattataa gatacccatc tgagatagac aattttttct caaaaacatc
     9721 tggaataacc aatgaaaaat ttaaaattcc ttcatcagct acaactgcga gaccaccaaa
     9781 attccgaaca actggttcat agccacgact gatgatttct tttttagcta attctaaatg
     9841 tggtaattct cgatctaaca tccccagaat gactgtctta gtcatcggcc aaatatgcaa
     9901 aataatttct tgatttgagc gattaatctc ccttaaaaat acttctgtcc atataaaagg
     9961 tttttccgcg tcttgagaat ctgtaacata gtctttaaaa atagatacag gtaactgcgc
    10021 tagatcttgc cattccaata aatctgacct cctttctcta gaaaactctg caagatttat
    10081 tttatcgcaa tcgcttgaaa ttttccagta agtttccctc aaattccatt actatcaaga
    10141 agaaatatat cttttattta cgaaaagcat ttttttatgt acaatatggc tatgaaaatt
    10201 ttgattacat caggtggaac taccgagaaa attgataccg ttaggagtat cacgaatcac
    10261 gcaacaggaa ctttaggaaa aataattgca gaaaaatatt tacgagaagg acatcaggtg
    10321 acactcgtga ctactaaaaa tgcagtcaaa ccagaatctg ctaccaactt atccatattt
    10381 gagattgagg acgttgactc attaataaaa accttgaagc cattagtgaa agagcacgat
    10441 attttaattc atagcatggc agtgtctgac tatacccccg tctacatggc cgatttcgaa
    10501 aaggttaaat caagcgatga tttagatact tttttgcgca aagataacca cgaagggaaa
    10561 atatcatctg aatcagaata tcaagtcctt tttctcaaaa aaacaccaaa agttatcagc
    10621 cttgtgaaaa aatggaatcc tcaaattact ctagtaggtt ttaaactact tgtgaacgtt
    10681 actaaagaaa atctttttaa agttgctcgt catagcttaa tcaaaaataa agctacgttt
    10741 attttagcta atgacctcat tgatattacc tcaaaacatc acatagccta cctacttgat
    10801 catgataatg tgtataaagc taccaccaaa gaagacattg ctcaattaat ttacgagaag
    10861 gtaaaaaaat atgattaaac gtattacact agccgtcacc ggttcaattt cggcctataa
    10921 agcagcagac cttacaagtc aattaactaa aattggctat gatgttcata tcataatgac
    10981 acaagctgcc acagaattta ttacaccgtt aactctgcaa gtattatcaa aaaatcctat
    11041 tcatttagat gttatggatg agcataaccc caaaatcatc aaccatatcg aattagcaaa
    11101 acgcacagat ttatttatcg ttgcacctgc ctctgctaat actattgctc atctagcata
    11161 tggcttcgct gataacatcg taacaagcgt tgctcttgct atgcctgatg aaactccaaa
    11221 acttatcgca ccagcaatga atacaaagat gtatcataat acaatcaccc aacgtaatat
    11281 tgatattttg aaaaaaatag gttatcagga aattgaacct cgtattagtt tgcttgcctg
    11341 tggtgatacc ggtcaaggag ccctagctga tatctctact atacttaaat gtatacaaga
    11401 agttgcaaat taggctgtaa atttgtatac tatttctagt ttaatttaag gagttataat
    11461 gaaaaagaaa aacaaatcct ctaacattgc tataattgca atcttttttg ctattatgct
    11521 tgtcattcat tttttgtcat catttatttt tagtttttgg ttagtcccca ttaaacctac
    11581 tttgatgcat atcccagtta ttattgcatc tatagcctat ggacctcgta ttggtgcaac
    11641 tctaggcgcc ttaatgggtg ggatcagcgt agctaacagc agcattgttc tattaccaac
    11701 gagttacctc ttctcacctt ttgttgaaaa tggtaatttt tattcgctaa ttattgcact
    11761 tgtaccacgt attctaatcg ggattattcc ttatttcgtt tacaaattac tacacaaccg
    11821 ctttggtttg gctatctcag gtgctatagg ctctctaaca aacacagtat ttgttttatc
    11881 tggaattttt atcttttttt caagtactta taatgggaat atcaagctaa tgctcgctgg
    11941 gattatttca tctaattcat tagctgagat ggtcattgca gctatcattg tatatctaac
    12001 tgttcctcgt attctcaata ttaaacatta aaaactggcc aggaagaaat cttcctggct
    12061 tttcttactt atattttata gtcatattga aaaaaacatg ttataatgaa agcgtttata
    12121 aatattgaag gagtatttca atgacttaca cagaaaattt tcaaaaatgg ttagattttg
    12181 agcagcttcc tgactatctt cgtcaagaat tactatcaat ggatgaaaaa acaaaagaag
    12241 atgcttttta cacaaactta gaatttggta ctgctggtat gcgtggttat attggtgctg
    12301 gtacaaatcg tattaatatt tatgttgtcc gtcaagcaac cgaaggattg gcaaaattaa
    12361 tcgaaactaa gggagaggaa gctaaaaaac gtggtgtagc tatcgcttat gattcgcgcc
    12421 acttctctcc agaatttgct tttgagtctg cacaggtctt ggcacagcat ggaattaaat
    12481 cttacgtatt tgaagctctt cgtccaacac cagaactctc ttttgctgtt cgccatttaa
    12541 atgcctatgc tggtattatg gttactgcaa gccacaatcc agcacctttc aatggttata
    12601 aagtctacgg acaagacgga ggtcaactac caccagctga tgctgatgca ctaacagact
    12661 ttatccgtgc tattgaaaat ccatttgcag ttgaacttgc tgatttagat gaaagcaaat
    12721 cagcgggtct tattcaagtt atcggtgaag atgttgatat tgaatatctt agagaggtta
    12781 aagatgttaa tatcaatcaa gatttaatca ataactttgg aaaagacatg aaaattgtct
    12841 atactcctct ccacggaact ggtgagatgt tgacacgccg tgcccttgct caagccggtt
    12901 ttgaatctgt tgttgtagta gaatcacaag ctaaggcaga tccagacttt tctactgtca
    12961 aatctcctaa ccctgaaagc caagctgcct ttgctcttgc tgaagaactc ggacgagaag
    13021 ttgatgcaga tgtcttagta gctactgatc ctgatgcaga ccgcctaggc gttgaaattc
    13081 gccaaccaga tggttcttat aaaaaccttt ctggaaatca aattggagcg attattgcga
    13141 aatacatcct ggaagctcat aaaacagcag gaacacttcc agaaaatgct gctctcgcta
    13201 aatcaatcgt atcaacagaa ttagtaacaa aaattgccga aagttatggc gctacaatgt
    13261 ttaacgtttt aactggattt aaattcatcg ctgagaaaat tcaagagttt gaagaaaaac
    13321 ataaccacac ctatatgttt ggttttgaag aaagcttcgg atatcttatt aaaccatttg
    13381 tccgtgataa agacgctatt caagcagttc ttttggttgc tgagattgca gcttattacc
    13441 gttcgcgtgg tttaacactt gcagatggta ttgatgaaat ttacaaagaa tatggttatt
    13501 ttgctgaaaa aacgatttca gtaacacttt caggtgtgga tggagctgct gaaattaaga
    13561 agattatgga caaattccgt gaaaatggtc caaaacaatt taacaatact gatattgtat
    13621 tattagaaga tttccaaaaa caaaccgcta ctaaaaatga tggaacaata tctaacttaa
    13681 caactcctcc aagtaatgtt ttgaagtaca cgttagctga tgattcatgg attgcagttc
    13741 gcccttcagg aactgaacca aaaatcaagt tctatattgc cacggtcggt aatgatttag
    13801 cagatgcaga aacaaaaatc gccaatattg aaaaagagat tacaacattt gtaaattaat
    13861 aa
//
LOCUS       DATRSD010000059        13314 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRSD010000059 DATRSD010000000
VERSION     DATRSD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.060"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(68..1405)
                     /gene="brnQ"
                     /locus_tag="VBD85_001426"
     CDS             complement(68..1405)
                     /gene="brnQ"
                     /locus_tag="VBD85_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7133977.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
     gene            complement(1601..3598)
                     /gene="metG"
                     /locus_tag="VBD85_001427"
     CDS             complement(1601..3598)
                     /gene="metG"
                     /locus_tag="VBD85_001427"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO7133978.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLGEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3741..4616
                     /gene="tehB"
                     /locus_tag="VBD85_001428"
     CDS             3741..4616
                     /gene="tehB"
                     /locus_tag="VBD85_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO7133979.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(4774..5469)
                     /locus_tag="VBD85_001429"
     CDS             complement(4774..5469)
                     /locus_tag="VBD85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7133980.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            5805..7022
                     /locus_tag="VBD85_001430"
     CDS             5805..7022
                     /locus_tag="VBD85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7133981.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYRYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTISAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            7006..7848
                     /locus_tag="VBD85_001431"
     CDS             7006..7848
                     /locus_tag="VBD85_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7133982.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            7925..8752
                     /locus_tag="VBD85_001432"
     CDS             7925..8752
                     /locus_tag="VBD85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO7133983.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(8792..9148)
                     /locus_tag="VBD85_001433"
     CDS             complement(8792..9148)
                     /locus_tag="VBD85_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO7133984.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKVSLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(9150..9626)
                     /locus_tag="VBD85_001434"
     CDS             complement(9150..9626)
                     /locus_tag="VBD85_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO7133985.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(9681..10862)
                     /locus_tag="VBD85_001435"
     CDS             complement(9681..10862)
                     /locus_tag="VBD85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEO7133986.1"
                     /translation="MVFSVKTFNNINQIGLQELGNRFQIDGDMSENPDAYIIRSQNLH
                     NQDFPSNLKAIARAGAGTNNIPIEEASAQGIVVFNTPGANANAVKEAVIAALLLSARD
                     YLGANRWVNTLTGTDIPKQIEAGKKAFAGNEIAGKKLGVIGLGAIGARIANDARRLGM
                     TVLGYDPYVSIETAWNISSHVQRVKEIKDIFETCDYITIHVPLTNETKHTFDAKAFSI
                     MKKGTTIINFARAELVNNQELFEAIETGVVKRYITDFGDKELLNQKGITVFPHVGGST
                     DEAELNCAIMASQTIRCFMETGEITNSVNFPNVHQIQTAPFRITLINKNVPNIVAKIS
                     TAVSELGINIDNIINRSKGDYAYTLIDLDETDNNKISTLIEEFEGDENIVRVRLIAKQ
                     Q"
     gene            complement(10925..11431)
                     /locus_tag="VBD85_001436"
     CDS             complement(10925..11431)
                     /locus_tag="VBD85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7133987.1"
                     /translation="MLLIEEARAEIAKTGSDQWQKEDGYPNRNDIIDDILNGYAWVGI
                     EDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISNQFRGRGLAQTFLQG
                     LIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLAYQKIKEKGETSIYR
                     EIDERNPM"
     gene            complement(11540..12632)
                     /gene="serC"
                     /locus_tag="VBD85_001437"
                     /pseudo
     CDS             complement(11540..12632)
                     /gene="serC"
                     /locus_tag="VBD85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
     gene            12765..>13314
                     /locus_tag="VBD85_001438"
     CDS             12765..>13314
                     /locus_tag="VBD85_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO7133988.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMV"
BASE COUNT         4376 a         2547 c         2076 g         4315 t
ORIGIN      
        1 atctgatata aaaaaggcta aaactaagtt ttaacctttt taacatatct ataaaattga
       61 aatagtgcta tccttcaaat ttctctaaat caaatgcttg gcctttttgt ttatctgaca
      121 gcaccaaaga aaatactata ccaatcaaca ttggaactag ccaccccatt gaaatagtat
      181 gaaaggggag gaatccaact aactgagtta atgttttttc ttgccattgt cccgctagaa
      241 cctcaacaaa cgacactaaa gtgacaagac caattgtcaa agacattccc ttttttgaaa
      301 gcggcaacca cttattaatc aaaataatta agacaataac aataacaatc ggatacaata
      361 aagttagcac aggaactgaa aaagtaatta cagcatttaa accaagatta gcaattaaaa
      421 agccaatcaa agtaaaaaca gtagcaaata atttataatt cccaaaacga aagttcttat
      481 caaaaaattc agatactgac acaataagcc caactgttgt tgtaaagcat gtcaatgtta
      541 ccataatact caagaaataa cgaccaaaat ttccaaataa tttataagat gcttgtgata
      601 atacataagc tcctttatta acatttgggt cagctaaaat atcagctggg acaggaaact
      661 tattccctaa aaaacctaag ccaatatata aaatactaaa tgctaaacta gtaacaattc
      721 caacaatcca aatggttgat aaatattctt ttttagtttt aaaaccaaac tttttaagcg
      781 tttctgttgc tactagacaa aaagcaactg ctgcaagtgc atcaagtgta ttatagcctg
      841 ctaagacacc acttccaaac gcttttcctg catagtcagc actcgcctta gcactctcca
      901 agtttccata agcaatagct ccaacaacaa ccaaagagag aattaaaaag gcaaaaacag
      961 gcgttaagat ttttccaaca ctatctaata taccatttgg tctaatagcg agataataag
     1021 ctgcagcaaa aaaacaggct gtaaaacaca aaagagcaat cggactatgc cctacaattg
     1081 gagcaacccc aatttcaaaa gaaaccgttg ctgttctagg aatagcaaaa agtggaccaa
     1141 tagttaggta gagtataact aaaaatgtta aagaaaacca cggactaaat ttttccgaca
     1201 tctctgtctt ataaccacca ttagttagtg tgcctagtag gagggtaata atagctaatc
     1261 caactcctga caaacaaaat cctaaaatcg ctggccagaa atcttgtcca gatgctacac
     1321 ctaatgctgg tggaaaaatg aggttccctg caccaaagaa gataccaaat aagagtaatc
     1381 ccgttaaaaa gccttttttt accataagca accattagtg taaataatct atttacaccc
     1441 tttccataaa ttctccttat caaattctat ctaaatgctt agaatcgatg tcttttatta
     1501 tatcaaaaat aaaaataaat tctaggtcat tttcataatt ttccaactat tcagtcactt
     1561 ttttcttgaa aacaaaaaaa gagcataatg ctcttttcga ttaaccaata attgatccat
     1621 ttgccactgc tgaatcgacc gtcaacactg tcaatttacc atcatgttct gctgagagaa
     1681 tcatgccttg actgacatat tttttcatca ttttacgtgg tttgaggtta gcaacaattt
     1741 ggagtttttt ccctactaac tcttgttcat ttgggtaaaa ttttgcaatc cctgataaga
     1801 tttgacgatg gccctcatca ccagcatcta gacggaaacg aagaagttta tcagaacctt
     1861 ccactttctc aacttcaatt acttctgcaa cgcggatttc aacagcgtca aaatcctcaa
     1921 acttgatttg acctttgctt gatgtcaatt caacttcttc aggaacccac tccttttcca
     1981 ctggtgcttt tccagcattc atttgttctt tgatataagt aatttcgtct tccatatcca
     2041 accttgggaa aattggtgaa ccttttgcaa caactcgaac accttctggt agatcagcaa
     2101 aagtcaattt ttcaagatcg aatgttgccc ctaaaccaag ttgttccata atggcatcgg
     2161 aagtttccat catgaacggt tgaatgaggt gagcaacaac acgtagacta gcaactaaat
     2221 gactcattac agctgctaac ttatcacgat ctgtttcgtc ttttgcaagt acccacggtg
     2281 ctgtttcatc aatatatttg ttagttcgtg aaataagatt ccatacagct tcaagggcac
     2341 gtgggaaatc caccgcttcc atttgtttat gatagttttc aatactatct gtcgcaacag
     2401 aagctaaatc ggcatcaaag tcagttgcaa cagcaaacct tggtacttct ccatcgaaat
     2461 acttgttaac catagcaata gtgcggttga gaaggtttcc taaatcatta gcaagttcat
     2521 agttaatacg tccaacataa tcttctggcg taaaagttcc gtctgaacca acaggcaatg
     2581 aacgcatgag ataataacgg agtggatcaa gaccaaaacg ctctactaac atttctggat
     2641 aaacgacatt cccttttgat ttagacattt taccatcttg catcacaaac caaccatgag
     2701 caactagacg ttttggtaat ggcaaatcca gcgccatcaa catgataggc caataaatag
     2761 agtggaaacg aagaatatct ttaccaatca tatgaatgtc tgctggccaa aatttatgat
     2821 attgactgag atcgtcagac caaccataac caagagcaga aatatagttc atcaaagcat
     2881 caatccagac ataaataaca tgttttggat ttgatggtac ctgaactccc catgtgtaag
     2941 ttgtacgaga cactgctaaa tcttctaaac ctggttctat aaagttttta agcatttcat
     3001 tcatacgacc atctggctga ataaattctg gatgctcagc gtagtacgct tttaaacgat
     3061 ctgcatattt gctcatgcgg aagaaataag attcttcaga aactttttca acttcatgac
     3121 cagatggcgc aacaccacca atcatattac cgttttcgtc acgataaact tctgctaatt
     3181 ggctttcagt aaaaaattct tcatctgaaa cagaatacca tccggtatac tcaccaagat
     3241 agatgtcccc ttgtgccaac aattgttcaa aaattttagc aaccgcttct tcatggtaag
     3301 tatctgttgt acggatgaat ttgtcatagg aaatatctaa aagttcccat aatgttttga
     3361 cactttcagc catgccatca acatattcct gtggtgtaat gcctgcttct tcagcctttt
     3421 gttgaatttt ctgaccatgc tcatcaagac cagtcaaata ctgaacatca aagcccatca
     3481 tgcgtttgta acgtgccaaa acatcacatg ctatcgttgt ataggcagaa ccgatgtgca
     3541 atttacctga tgggtaataa attggggtag taatgtaaaa tgattttttt tctgtcatga
     3601 tttctcctat aagccaccct aatgagaggt gttttttatc atgatacatt ataacataaa
     3661 atctttttca actggatata tctctgaata atatatctac ttttgctata ataaaggtat
     3721 taaaaataga gagttaatat atggttaata caaaaacact tctcccctac aaaactatgc
     3781 cggtttggac tgcacagtct attccaaagg catttttaga aaagcataat actaaggaag
     3841 gcacctgggc aaaactaacc attctaagtg gttctttagt attttaccag ttatctcctg
     3901 atggagagga aatctcgcgg catatttttg atgctagtag tgatattcct tttgttgatc
     3961 cacaagtctg gcataaagtt tcgccgaata gtccagactt aagttgctat ctaacttttt
     4021 actgccaaaa agaagattac ttccataaaa aatatggtct cacgcgcaca cattctgagg
     4081 ttatcgccag tgcacctctc ttatctgaga agagtaatat attagacctt gggtgtggtc
     4141 aagggcgaaa ctcactttat ttatcgctgc tgggacatca agtgacttct gtcgattcaa
     4201 acggacagag ccttgtagct ttagaaaata tggcattaga agaagagctt ccttacaata
     4261 taaaaaggta tgatattaat actgctgcta ttgaagggca ctatgatttt attttatcaa
     4321 ctgtggtatt tatgttttta aatcccgact gcatttctga tattatttta caaatgcaat
     4381 cacatactca gattggagga tacaatctta tcgtttctgc tatggatacc gctgaaaatc
     4441 cttgcccgct tccattccct tttaccttca aagaaggaca attgaaatct tattacaatg
     4501 actgggaaat catcaaatat aatgaaaatt taggtgaatt gcacagggtg gatgaaaacg
     4561 gtaatcgctt aaaattacag tttgcgactt tattagctcg aaaattaaga tcataaaagt
     4621 gagtagaaat ctactcactt ttatctttgg ttaggtaata agtatattct aaacccatac
     4681 ataattatcc tataaaatat atagagatga tatttcaaat cattattgta acaatcagct
     4741 ttagtatagc tcttcaacat aaaacgccat acattaggag tgcctttcta cttgtaggtt
     4801 ataaaattca tggcctaaca gcaagttgac atataaaaga ggaattaata ttaatgataa
     4861 gctccattcg cccagtttac ttgaaaaata agtcgagcaa acaacaccaa agataaatcc
     4921 tccaataata ataatcgtat ttctagcaat taagaaaata tcagaatttt tttcgtaaag
     4981 tccctttgtt aataaataag cagcattctt gatattacca gtcatcatca tattagcata
     5041 aggtgctcct ctgagcgatt taaaggtatc tacttgtatt gaagcaaaga aggctagtcc
     5101 tgccacagta aacgacgatg gtaagaaagg agtcaatata attgttacta tagcaacctg
     5161 tgtcaacgca aaactagata ataggtacca gtgcagttga tgtttattcg cccaacgatt
     5221 catgaaataa gtaaacgatt gacctaacat gaaaaccatt ataggtagca aaaaagcaag
     5281 cgcttgacta taatgcttat tactaagatg aatagcaaaa gataacaaat tccctgtttg
     5341 tacacctgca aaacgttttc cttgtgttac ataagtaaaa gcattgatat aacctgaaat
     5401 aaaacagagt gcaaccgcac acctgagccc ttcatatatt tgatattgac ggtgagactt
     5461 tttagacata ttttcctcta actttatggc aaacaaaaat aagactgggc aatcttgctc
     5521 agcacttttt ttctaataga ttggtttaaa atagtattaa tattagcact agctagccta
     5581 acagttctaa ataaattatt aagcaatgat aaatgtagtt ttagtatatc ataacctcag
     5641 cattgaagca atctcacttt atgaaaggat aaagaaaatc tcataagtca tttaccctta
     5701 atttgaagtt taattagtta cttttaattt tactaccaaa aaacttgcac cattcttaac
     5761 atttgttaca ataaatacaa cctattagga ggtcagtaca gattatgcac agcactattt
     5821 ataaatgtca tcattgccgt tatctaattt ataaaagttt taaagatatc tttccttttg
     5881 ctctcatttt ttcttggatt gaaatccttc aaaaactcgt tatatcagat gattcttttt
     5941 tagctcaaac aacaaagtcc agtatttata aaaacgagtg gttatatacg gtttccaata
     6001 ctctttatga tttatctcat ttactaatct actttatggc tagttatttt gtggctagtt
     6061 tagtatggag aggcttatct aaccaaacta aagaaagttt ttctgcaagt ttacttggct
     6121 ttgccactgt ttggttgctt ctaacttcag ttgttccagg tacagattat cactatccta
     6181 gccaacccat ttggcttatg ctggctttga tttcgtatct ttttatcttt ttaactcgac
     6241 tgcttcaaaa tcgacatcat attttccagt atagtatgtt agctatacta ctattaatct
     6301 cttctatttt gtcacatatc ttatatcgtt atcctaatat tgatttagaa taccttatac
     6361 aaaatcactt ttctcaatgg ctgggtgatg ggccacatca attaattcct attatgcttt
     6421 ggtctatctt agctattttt ctaaccttat caggtctttt tgtaccagat attttatttc
     6481 gtccttactc ctatttttca gtagttagcg aaaatttaaa tgctgccttg agccaacata
     6541 cagataaaat cccttatctg tatacctttt atactgttaa aaatagcttt gctatgtttg
     6601 gcggtattgg aatcctatta tccttatttt tagcagtttt atatgaaagt agaaaactac
     6661 aatcgaagaa ttactataaa ttgacattac tgaccttaac tcctcttatt tttgatcaaa
     6721 atttaccatt tttagtaggt ctacctgtca ttttacaacc aatattattc attccgatgg
     6781 tattaacaac tatctctgca gaggcttttg gagctcttat gctctaccta aaatttgtcg
     6841 accctgcagt ttatactgtc ccaagtggta cacctagtct actatttggc tttcttgctt
     6901 caaatgggga ttggcgttac cttccagtca ctgcaattat tctagttgtt ggcttcttta
     6961 tttatcgtcc gtttgtcaaa atcgctttcg ctaaggagga gcaatatgaa aaaattactt
     7021 agatggcttc ctcctgtact tttcattatt atccttatag gaatgactat cttaggtaag
     7081 tcctatatca ataaagtaac agctcacaaa ataaaactct ataactctcg aatgactcct
     7141 actattttaa ttccaggatc cagtgctact caagaacgat ttaacagcat gttagcacag
     7201 ctcaaccaaa tgggagaaaa acatagcgtt ttaaagttaa ctgtcaaaaa agacaatagc
     7261 attatctaca atggacaaat tagcggcaat gaccacaaac cctacattgt cattggattt
     7321 gaaaataatg aagatggtta tagtaacatc aaaaaacaaa caaaatggct acagattgct
     7381 atgaatgatc ttcagaagaa atataaattt aaacgtttta acgctatcgg tcattcaaat
     7441 ggtggcttat catggactat tttcctagaa gattattacg actctgatga atttgatatg
     7501 aaatcattgt taacaatggg aacacctttt aactttgaag aaagtaacac ctcaaatcat
     7561 actcaaatgc ttaaagattt aatcagtaat aaaggaaata ttccatcaag tctcatggta
     7621 tacaatttgg caggaactaa ttcatatgat ggtgataaaa ttgttccatt tgctagtgtg
     7681 gagactggta aatatatttt ccaagaaacc gctaaacact atacccaact aacagtaact
     7741 ggtaataatg ctacacattc tgacttgcct gataatcctg aagttatcca atatgtcgca
     7801 gaaaaaattc ttaaaaatga gaaaggtaaa ttaccaaaac ctcactaaag ctgtaaaata
     7861 gcaacgcctc tggtattttg atataatata ttgcgaatta aactaaataa aaaaggatat
     7921 tattatgaaa ctcatttcat ggaatattga ttccctcaat gcggcattaa caagtgaatc
     7981 aacacgtgct ttaatgtcac gccaagttat tgatacttta gttgctgaag atgccgacat
     8041 cattgcgatt caagaaacta aactgtctgc caaaggacct actaaaaaac atttggaagt
     8101 acttgaaact tacttcccag aatatgattt ggtttggcga tcatcagtcg aacctgctcg
     8161 taagggctat gctggtacaa tgttcttata tcgtaaaggg ttaaatccta ttgttagctt
     8221 ccctgaaatc gatgctccta ctacaatgga taatgaaggt cgcattatta ctctcgaatt
     8281 ggaaaactgc tatatcacac aggtctatac tccaaatgca ggtgatggcc taaagcgctt
     8341 agctgaccgt caaatatggg atattaaata tgctgagtat ttagcaacat tggatagtca
     8401 aaaacctgtt ctagcaacag gtgactataa cgtagcccac aaggaaatag acctagctaa
     8461 tcctagcagt aatcgtcgtt ctgctggatt cacagctgaa gagcgccaag gctttacaaa
     8521 tctactagcc aaaggtttta cagatacttt tcgttacctt catggcgatg ttccaaatgt
     8581 ttatagctgg tgggcacaac gaagtcgtac atccaaaatc aacaatacag ggtggagaat
     8641 tgattattgg ctaacaagta accgtgtcgc agataaaatt accaagtcag aaatgattca
     8701 ttctggtgat cgtcaagatc atacaccaat tattttggag attgaactat aaaccaaaaa
     8761 aagatgattg agttataact caatcatctt tttatactaa gttgaggtct ttatacttag
     8821 tacggtagcc aatctgaaga attttattat ctttaactag caatggacgc ttaataagca
     8881 ttccatccga tgccaataag tccaaagctt ggtcaagagt taaatcatca aatttatctt
     8941 ttaagcctaa ttctctgtaa gaatttcctg aagtattgaa aaacttttta aggtcataag
     9001 gcgaattctc taacaattct tttaataacg aaactttggg aggattgctt tttatatcaa
     9061 tcgcttcaaa agtcaagccg agttcagtta actctttttt tgctgaacga caagtggtac
     9121 atttggggta ttcataaaac gtatacatat tatttctcct tttcgtattc taataaccaa
     9181 gattttcttt ctaagccacc agcataacca gttaattgac catatcttcc taaaacacgg
     9241 tgacaaggta taattagaga aataggattt ttacctattg ctcctccaac agcctgtgca
     9301 gattgacaat ttagctcttt cgctaattct ccataagttt tggtatctcc aaaagggatt
     9361 ttctgtaatt cctgccatac tctcgtctga aatgctgtcc ctctcaatga taatggaata
     9421 ctaggtaaat tttgctgtga aaaataagca tctaaccact tcttaccaag taaagtatag
     9481 gaatttgaca tattaacaat ttcctcttgg ttgactgcta gcatatcata tttttgccca
     9541 acaaaataaa gccctgataa acctagattg tctgctagaa ggcgaatttc tccaagaggg
     9601 gactgataga attcttgata taacatatga taagcataac ttaaaaagcc atgaaaagca
     9661 attcatgact ttttagttct ttattgttgt tttgcaatta aacgtacacg gacgatattc
     9721 tcatcacctt caaattcttc aattaaagta gaaatcttgt tattatccgt ttcatctaaa
     9781 tctattagtg tgtaggcgta gtcacccttt gaacggttaa taatgttatc aatatttata
     9841 cctaactctg atacagcagt tgaaatttta gccacaatgt taggaacatt cttgttaatt
     9901 aaagtaatac gaaatggtgc ggtttgaatt tgatgtacat tggggaaatt aactgaatta
     9961 gtgatttctc ctgtctccat aaaacaacga atggtttgac ttgccataat agcacaattt
    10021 agctctgcct catctgttga tccgcctaca tgaggaaaga ctgtaattcc tttttggttt
    10081 aataattctt tgtctccaaa atcagtaata tagcgcttga caacaccagt ttctatcgct
    10141 tcaaatagct cttggttatt gactaattct gcacgagcaa agttgataat cgtagttcct
    10201 tttttcatga ttgaaaaagc tttcgcatca aaagtatgct tagtttcatt tgttaaggga
    10261 acatgaattg tgatatagtc acaagtttca aaaatatcct taatctcttt aaccctttga
    10321 acatggcttg aaatattcca agctgtttca attgaaacat agggatcata accaagaact
    10381 gtcattccta agcgtctagc atcattcgca attctagctc caatggcacc aaggccgata
    10441 actcccaatt tttttcctgc aatttcatta ccagcaaaag ctttctttcc tgcttcaatt
    10501 tgtttgggaa tatctgttcc agttagagta ttaacccatc ggttagctcc taaataatca
    10561 cgagctgaaa gtaataaggc agcaatgacc gcttctttta cagcattagc atttgcacct
    10621 ggggtattaa acacgactat tccctgtgca cttgcctctt caataggaat attatttgtt
    10681 cctgcacccg ccctagcaat agctttgagg ttacttggga aatcctgatt atgcaaattt
    10741 tgactacgaa taatataggc atcaggattt tctgacatat ccccatcaat ctggaaacga
    10801 ttccctaact cttgtaaacc aatttgatta atattattaa atgttttgac actaaaaacc
    10861 attctttttc actaaggtta tctctttagt catcattgac caaaataacc cctttctttt
    10921 tttcttacat gggatttctt tcatcaatct cacgatagat gctcgtttct cccttttctt
    10981 ttatcttctg ataagccaat cgtaccccat ctagaggtac tttaccacaa tattgatagc
    11041 ctaatttatt tagaatatgc tgcatagtga catttttttc atgagtatcg caacgaaaat
    11101 caggtccttt atgaccttca attaaccctt gaaggaatgt ctgtgctagt cctctaccac
    11161 gaaattgatt tgagatcgca atacgatgaa atgtaagata gcgatggtta tcatggagcc
    11221 attttccctc ataaatagca tcataaacct cttcatgacc atcaatgaca gccgcataag
    11281 tcgctagcat cccatcttca ataccaaccc aagcataacc gttcaagata tcatcaataa
    11341 tatcgttacg attagggtat ccatcttctt tttgccattg atcacttcct gttttagcaa
    11401 tttcggcacg cgcttcctca ataagaagca taatttggtc aatttcattt ggaaagctaa
    11461 tctgatttgc attatattac ccacttttat atattataga ctataataac ggtttaattt
    11521 tctttcgtga taacatttac tatgtatttt ctgcttcaaa tgttttcata aaatcaatta
    11581 attcgatgac accttgttta ggaaaggcat tataaaggct agctctcata ccacctaccg
    11641 aacgatgtcc tttaatattt ttaaatccac gttctgtcgc ttcctttaca aatttttcat
    11701 ctaaatcttt attaatagtt atgaaaggaa tgttacatag agaacgactc tttttatctc
    11761 tgacaggatt actataaaat tctgaggaat caatataatc atagagaagt cctgattttt
    11821 cacgattagc tttttccata gcatcaacac cacctagact tttcacccat tcaaagacta
    11881 atttagcaat gtaaatactg taagcaggcg gcgtattata aagagaacca gcatctgact
    11941 gaatcttata atccaacatt gatgataaag ttggttcctc attaatcata tcttcacgga
    12001 taataactac cgttacacca gcaggtccaa tatttttttg agctcctgca taaatcattg
    12061 caaaatcttc tactttatat tttacagcta atatattaga agacatatcc gcaataacag
    12121 gcacttcatt agttttagga atatcataaa gcgatgttcc ttcaatggta ttatttgttg
    12181 tcacatgaac ataggcagct ttaggatcaa tctctttttc atcaaactct ggaatataat
    12241 cataaactga ttcttctgat gaagctaata gaattggttc aaaaggaatt gtttttgata
    12301 gttttacagc ctctgtataa gcttttttcc ccccacgaac cagcaacatg atagtaagct
    12361 ttacgccctc tggcaatatt caagggaatc atactaaact gtaaggatgc accaccttgt
    12421 aaaaagatta ctttataatt atccggaatt cccatcaaat ctcttaagta acgctcagca
    12481 cctttgataa tatcatcaaa ctctttggag cggtgcgata cctccaaaac actcatacta
    12541 gacccttgat aattaaggag ttcagattgg gcttttacaa gcactggttt tggtaagact
    12601 gcaggacctg cagaaaaatt ataaattgtc atataacacc tccatataat tgtgtaagat
    12661 tatagcacag tattttgaat attgtaaaga tttttttgat taattttgct ttttattcgt
    12721 gatataatgg taaaggcgaa caatcaagtt taaaggagta atttatgatt ttgagggaat
    12781 tttgtgcaga gaatttaaca gacttaacca gattagataa agctattatt tcacgtgtcg
    12841 agctttgtga taaccttgct gtcggaggca ctactcctag ctacggtgtt atcaaagaag
    12901 ctaatcaata tttacatgaa aaaggtatta gtgtcgctgt tatgattcgt ccccgtggag
    12961 gaaattttgt atacaatgat ttagaattac gtattatgga agaggatatc ctacgtgctg
    13021 ttgagttaga atcagatgct cttgttttgg gaatattaac ttcaaataat catattgata
    13081 ctgaagccat tgaacaactt ctacctgcta cccaaggttt gccacttgtt tttcatatgg
    13141 catttgatgt tatcccgaaa tctgaccaaa aaaaatcaat agatcaatta gttgctttag
    13201 gctttacaag gattctccta catggttcat ctaatggcga accaattata gaaaatatca
    13261 aacatatcaa agcattagtt gaatatgcta ataatcgtat tgaaattatg gttg
//
LOCUS       DATRSD010000060        13221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRSD010000060 DATRSD010000000
VERSION     DATRSD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.061"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            5..78
                     /locus_tag="VBD85_001439"
     tRNA            5..78
                     /locus_tag="VBD85_001439"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            complement(294..1646)
                     /locus_tag="VBD85_001440"
     CDS             complement(294..1646)
                     /locus_tag="VBD85_001440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO7133989.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFVVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVINSKDEKRAIKALYETFFQK
                     "
     gene            1740..2390
                     /locus_tag="VBD85_001441"
     CDS             1740..2390
                     /locus_tag="VBD85_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7133990.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITIDHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGITAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            2527..3318
                     /locus_tag="VBD85_001442"
     CDS             2527..3318
                     /locus_tag="VBD85_001442"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO7133991.1"
                     /translation="MKFENIIYGIDGNVATIMLNRPDISNGFNIPMCQEIIDAIRLVS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKAAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            3414..3848
                     /locus_tag="VBD85_001443"
     CDS             3414..3848
                     /locus_tag="VBD85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7133992.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            3848..4819
                     /locus_tag="VBD85_001444"
     CDS             3848..4819
                     /locus_tag="VBD85_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO7133993.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSYLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            4877..5101
                     /locus_tag="VBD85_001445"
     CDS             4877..5101
                     /locus_tag="VBD85_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7133994.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            5256..6215
                     /gene="fabK"
                     /locus_tag="VBD85_001446"
     CDS             5256..6215
                     /gene="fabK"
                     /locus_tag="VBD85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO7133995.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            6235..7161
                     /gene="fabD"
                     /locus_tag="VBD85_001447"
     CDS             6235..7161
                     /gene="fabD"
                     /locus_tag="VBD85_001447"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO7133996.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIELRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVARRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVHSVEDKIGFNNLKELN"
     gene            7170..7904
                     /gene="fabG"
                     /locus_tag="VBD85_001448"
     CDS             7170..7904
                     /gene="fabG"
                     /locus_tag="VBD85_001448"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO7133997.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            7920..9152
                     /gene="fabF"
                     /locus_tag="VBD85_001449"
     CDS             7920..9152
                     /gene="fabF"
                     /locus_tag="VBD85_001449"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO7133998.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALVEANIKPEEVNYVNAHGTSTPANEKGESQAIVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            9118..9654
                     /locus_tag="VBD85_001450"
     CDS             9118..9654
                     /locus_tag="VBD85_001450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO7133999.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            9651..10073
                     /gene="fabZ"
                     /locus_tag="VBD85_001451"
     CDS             9651..10073
                     /gene="fabZ"
                     /locus_tag="VBD85_001451"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7134000.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            10111..11481
                     /gene="accC"
                     /locus_tag="VBD85_001452"
     CDS             10111..11481
                     /gene="accC"
                     /locus_tag="VBD85_001452"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO7134001.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARTEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIVTNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            11490..12365
                     /locus_tag="VBD85_001453"
     CDS             11490..12365
                     /locus_tag="VBD85_001453"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO7134002.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            12358..13131
                     /locus_tag="VBD85_001454"
     CDS             12358..13131
                     /locus_tag="VBD85_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO7134003.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEVLSQLSLEDLLEQRYQRFRKY"
BASE COUNT         4244 a         1972 c         2822 g         4183 t
ORIGIN      
        1 ataatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gataaaggga gactaacttt ggttagcctt tttttattgg
      121 aattattatc cttaagattg gctaacatct taattgtccg aatttgtcac tgataccaag
      181 tggttacgat taaaagtgga aaaactatcc tatgttctaa ataaaatcgt cctaagtaag
      241 cttacttagg acgatttgtt atctatctaa tagtgtaagt agtgtaatag gtactatttt
      301 tggaagaatg tttcatatag tgctttaata gctctttttt catccttact gtttataacg
      361 aacataatgg agacttcgct tgaaccttgt gatatcatgg tgatattgat tttttctctt
      421 gataaggctt gtgtcgctgt tgcagtgact ccaatctgac ttttcatatt ttcacctaca
      481 attacgattg tagatagatt gtgttggatg tcaacgtaat ctacttctag tttacgagtt
      541 aggtaattta agatttcttg ttctttgatt ggtgtcaatt ctttttcacg taagacaatg
      601 gatagatcat ctatgccagt tggcatatgt tcaaaactaa tatttaaatc ctctaaaatt
      661 tgtagtactt ttcggccgaa acctacttct ctattcatta agtatttaga tacgtttatg
      721 ctagcaaaac ggctatcaga agcgatccca gttactgcta tgttactacg agtatgcttt
      781 aaaactattt ttgtaccagg ctgttgggga ttatttgtat ttttaataac aagagggatt
      841 ctgccacgat aggcaggaag taaagcttca tcatgtaaaa ccgaaaaacc cgcataggct
      901 aattcacgca tttctttata agtaagctca gggatagcgt gagggttctt aactacacct
      961 ggatgtgctg caaatatacc atcaacatct gtgaagttct cataaaggtc tgcttttatg
     1021 cctgctgcaa ttagggatcc agtaatgtca gatccccctc ttgaaaaggt acaaatttgg
     1081 ttatcttctg tgactccaaa aaatccaggg ataacaagaa cctcattgta tagacataag
     1141 ttctcaatct tatcataact tcctggaata attcgtgcat tacatggttc tttagttaca
     1201 ataattcctg cttcgtttgg atgaacatat cttgcaggaa tgtcattttg attaaaaaac
     1261 gttgcaacta actttgcgtt gttatcttca ccggctgcta aaaaacaatc atataaaaac
     1321 tgattatttt ctatgggaag tgaagctagc tgctctatag agcgagtaat ttcttctgca
     1381 atagtgcttc ctaaagacaa atcactaata atttcttgat aacgattgat aatccaagtt
     1441 tggtctttaa caatatcttt accgttaata taattttgat aatattgtat caaagcatct
     1501 gtcattttta agtcttcttc gtagcgtttc cctggagctg aaacgacaac aaaccgtcta
     1561 gtgtagtctg atttgataat atttagtact ttgtataatt gttgggaaga tgctaaagaa
     1621 cttcctccaa atttgactac tttcataggt tacctcaaga atgaatttta gataaattat
     1681 aacaaaagtc agaaaatttg tcattacctc taaatttaaa agaatgatat aatagttaca
     1741 tgtgtaaata taaagcaata atatttgata tggatggtgt tctatttgac actgaattat
     1801 tttattataa aaggcgagag cgttttttaa aacaacatgg aattacaatt gaccatttac
     1861 caatgaattt ttttattgga ggaaatatga agcaggtttg gaaatctgtt ttaggtgatc
     1921 aatatgatac ctgggatatt gataagctcc aacaagatta tagtcgctat aaagaggata
     1981 atcccttgcc atacaaagat ttaatttttc aggattgtaa aagagtcatt gaaaagcttc
     2041 atcacaaagg ctatcttttg ggtctagctt caagttctac tagacatgat atcatgttag
     2101 cattagaaag ttttaatctg gatacatatt ttaaggtaat tctgtctggt gaggaatttt
     2161 cagaaagtaa acctaatcca gctatttata atagagcagc agaactttta gatattccta
     2221 aacagcaaat tcttattgta gaagacagtg agaaggggat tacagctggt attgcagccg
     2281 gtattgatgt gtgggctatt gaagataaat actttggaat gaatcagtcg caggctaatg
     2341 tattagtgag cgatctgagt caattttttg tgaaattaga taactattaa aaacgggaag
     2401 tttaattccg tttttttatt ttttatcttg ccatgaaagc taggtttatg ataaaataca
     2461 aagcatgata atttgataat caaattattt tttatttttc tttcactctt aaagagagga
     2521 gacattttga agttcgaaaa tataatatat ggtattgatg gcaacgtggc cactattatg
     2581 cttaatcgac cagatatttc aaatggtttt aacatcccaa tgtgtcaaga aattattgat
     2641 gcaataaggc ttgtgtctga aaataaagat gtcatgtttt tagtgattga agcacaagga
     2701 ccaatctttt caatcggagg agatttaaaa gtgatgaaag ctgctgttga gagcgatgat
     2761 attagctcat taactaaaat tgctgagtta gttaatcaga tatcgtatga tttactacag
     2821 ctagagaaac ctgttgttat gtgtgtagat ggggctgtag ctggtgccgc agctaatatt
     2881 gcattagcag ctgattttgt tatcgcaagc aagaagagta aatttatcca ggcttttgtg
     2941 ggtgtcggtt tagctccaga tgcaggaggt ttattgttac ttagtaaatc aatcggtata
     3001 actcgtgcag tgcaactagc tttaactgga gaaagcttat ctgctgaaaa agctgaagct
     3061 ttgggaattg tttataaact ttgtgaatca gataaaatag gtaaaattaa ggatcaacta
     3121 ttaaagcgtt taagtagaca ttctataaac tcatatcaag ccataaaatc attagcttgg
     3181 gaagctgctt ttaaagattg ggaacaatat aaaaagcttg aattacaact gcaagaatca
     3241 ttagccttta agcaagattt taaagaaggt gttcgggcac acgctgatag aaggcgtcct
     3301 aattttttag gcaaatagtt tgaaaatcaa attatttaag attaaagtct tgcaaataag
     3361 tttgaacttt gatataattt gattatcaaa gtttaatgtg aggtggtgat atcttggaat
     3421 acgatcagat taatagttat ttagttgaca tttttaatag aatcatgatt attgaggaaa
     3481 tgagtttaaa gactagccaa tttagcgatg tatctttaaa agaaatgcat actattgaaa
     3541 ttattgggaa acattctgag gtaactccta gcgatgtcgc tcgagaatta atgttaactt
     3601 tgggaactgt aacaaccagc cttaataagt tagaaaaaaa aggctacatt gagcgtaaac
     3661 ggtcaagtat tgatcgtcga gttgttcact tgagtcttac taagagaggt aggttattag
     3721 atcgattgca tagtaaattt cataaatcta tggtatctca tattattgaa gacctaggtg
     3781 aagaagacat aaaaatgttg acgagcgctt tagggaatct tcataaattt ttggaggact
     3841 tagtctagtg gtttttgcta aaattagtca acttgcacat tatgcacctt ctcaaattat
     3901 taaaaatgag gatttatcat taatcatgga taccagtgat gactggattt cgtcacgtac
     3961 tggtataaaa caacgtcata ttagtaaaaa tgaaacaaca gctgatttgg caaataaggt
     4021 cgcagaacaa cttattgaaa aatccggtta ttccgctagt caaattgact ttattattgt
     4081 tgcaacgatg acaccagact ctatgatgcc ttctactgct gcaagagtgc aagctcatat
     4141 tggagcgagt aatgcttttg cctttgactt gagtgcagca tgtagtggtt ttgtttttgc
     4201 cttgtctacc gccgaaaaat tgatatcatc aggtagctat caaaaaggtt tagtaatagg
     4261 agcagaaact gtttctaaag tattagactg gactgataga gggacagcag ttttatttgg
     4321 agatggagct ggaggtgtct tgctagaagc ttctaaggaa aaacattttc ttgcagagtc
     4381 tctcaataca gatggtagtc gtcagggact tcaatccagt caagtcgggt taaattcacc
     4441 tttctcagat gaagtacttg atgataaatt tttgaaaatg gatggtagag caatctttga
     4501 ttttgctatc aaagaagttt caaaaagtat caatcatctt atagaaacat cttatttgga
     4561 aaaagaagat attgattatc ttttcttaca ccaagcgaat cgaaggattt tagataagat
     4621 gtctcgaaaa attgatatag cacgtgataa atttccagag aatatgatgg attatggtaa
     4681 tactagtgca gctagtatcc ctattttact ttcagaatct tatgagaacg gtttgttgaa
     4741 actagatggt aaccaaacaa tcctcttatc gggtttcggt ggaggtttga catggggaag
     4801 tctaattgtt aaaatttagt tatatatagt gcatagcaca tttataaaag attatttaaa
     4861 atttaaggag ctaaaaatgg cagtatttga aaaagtacaa gaaattattg ttgaagaact
     4921 tggtaaagat gcagaagaag taacgttgaa cactactttt gacgatcttg atgctgattc
     4981 gcttgatgtt ttccaagtga tttcagaaat tgaagatgca tttgatatcc aaattgagac
     5041 agaagaagga ctaaatactg ttggtgattt agttgcttat gttgaagaaa aagtaaaata
     5101 attaagtcgt aagaaaaaaa gaagcaagtg ataataaaag atattcttac caaatcactt
     5161 attagagtga gtattcaatc aatcgttgat cggtactcac tcttcttctt atcaatatag
     5221 tttgattgtc aaattattaa ttttaggaaa gaaaaatgaa aacacgtata acagaattac
     5281 taaatataaa atatccaatt tttcaagggg gtatggcgtg ggtcgctgat ggagatcttg
     5341 caggagcagt ttctaaggca ggtggtctag gtattattgg tggaggaaat gcccctaaag
     5401 aggttgtcaa agcaaatatt gataaaataa aatctatgac tgataaaccc tttggtgtga
     5461 atattatgct tttatcacct tttgtagatg atattgttga tttggttatt gaagaaggtg
     5521 taaaagttgt aacgacgggt gcaggtaatc ctggtaagta catggagcgc tttcatgaag
     5581 caggtataac agtaatccct gttgttccta gtgttgcatt agcaaaacgt atggagaaat
     5641 taggggctga tgctatcatt acagaaggaa tggaagcggg tggtcacatc ggaaaactga
     5701 ccacgatgac attagtacga caggttgttg atgctgttac aattccagtc attgccgcag
     5761 gtggtatagc agatgggcgt ggggctgctg cgggtttcat gttaggagct gatgctgttc
     5821 aggtaggaac tcgttttgtt gttgctaaag aatctaatgc tcaccctaat tacaaagcaa
     5881 aaatcttaaa agcaaaagat atcgacactg ctgtttctgc tcaggtagta ggtcatccag
     5941 ttcgtgcctt aaaaaataaa ctagtaacaa cctatagcca agcagaaaaa gattatttag
     6001 ctggccgtat ttcaatcaat gagattgaag aattaggagc gggagctctt cgcaatgcag
     6061 ttgttgatgg ggatgttatt aatggttcag ttatggcagg acaaattgca ggattaatta
     6121 aatcagaaga gacttgtcaa gagattcttg aagatattta ttcaggagcg cgtcaagtta
     6181 ttctttctga ggcaagccgc tggtcagatt tataaataca aataaggaga cactatgaat
     6241 aaagttgcat ttctatttgc tggtcaaggt gctcaaaagt taggaatggc tagagattta
     6301 tatgaaactt tcccgattgt taaagaaact tttgataagg caagtcatgt tttaggatat
     6361 gatttgagag aattgattga taaagactta gacaaattaa atcagacgaa atatacgcaa
     6421 ccagctattc taacgacttc gactgctatt tatcgtttaa ttcttaaaga aattgaactt
     6481 agaccagata tggttgcagg actttctttg ggagaataca gtgctttagt tgcttcaggt
     6541 gctataagat ttgaagatgc tgtagtactt gttgctagac gaggccaact aatggaagca
     6601 gcagcacctg ctggtagtgg caaaatggta gcagttttaa atgctgatcg tcaaataatt
     6661 gaagatgctt gtaagaaggc ttctcagttt ggtattgtta gtcctgcaaa ttataatact
     6721 ccaaagcaaa ttgttatagg gggagagtca atcgctgtaa atgcagctgt agaagaatta
     6781 aaacaacaag gagtaaaacg tttaattcct ctgaatgttt caggtccttt tcacactgct
     6841 ttattgaaac ctgcaagtca gaaattgagc gatgttttag ataaagttca tttttcagtt
     6901 tctgaaatcc ctgtaattgg aaatacagag gcgcagataa tgaaaaaaga cgatatcaaa
     6961 tctttattgg cacgccaagt tatggaacct gtacgctttg atgagtctat tgaaaccatg
     7021 aaaaaaatgg gaatgacaca ggttgttgaa attggtccag ggaaggtact ctcaggtttc
     7081 ttgaaaaaaa tagatagctc attatcagta cattctgttg aagataaaat tggatttaat
     7141 aatcttaagg aattaaacta ggggaaaata tgcaattaaa agataagaac atttttatta
     7201 cgggttcaag tagaggaatc ggccttgcaa ttgcccatca atttgcacaa ttaggtgcta
     7261 atattgtttt aaatggacgt tctgaaatat cagaagactt aatagcagaa tttgctgatt
     7321 atggtgtaaa agttattgct atttcaggag atgtttcttc ttttgaagat gctaatcgca
     7381 tgattaaaga agctattgca agtctaggta gcgtggatgt gctcgttaac aacgctggta
     7441 ttactaatga taaattgatg cttaaaatga ctgttgaaga ttttgagagc gttcttaaaa
     7501 ttaatttaac aggtgcattt aatatgacac aatcagtctt gaaaccgatg acaaaagcgc
     7561 gtcaaggtgc tattattaat atctctagcg tcgttggatt aactggaaat gtagggcaag
     7621 ctaattacgc agcctctaaa gcaggtttaa ttggctttac aaaatcagtt gccagagaag
     7681 tagcagcacg aggcattcgt gtcaatgcca ttgctccagg ctttatcgaa tctgatatga
     7741 cagatgttat cccagaaaaa atgcaagaag ctattttagc tcaaattcca atgaaacgta
     7801 ttggaaaagg taaagaagta gcgcaggtag cgtctttctt agcagaacaa gaatacctta
     7861 caggacaagt tattgccatt gatggcggta tgacgatgca atgataaaga ggaaataata
     7921 tgacattaca gcgagtagta gtaacaggtt acggtgtaac ctcacctatt ggaaacacac
     7981 cagaagaatt ttggaatagt cttaaagaag gaaatgttgg tattggacct atcactaaat
     8041 ttgattcaag tgatttcatg gtcaaaaatg ctgctgaaat tcatgatttt ccatttgata
     8101 aatattttgt caaaaaagat ctgaacagat ttgatatgta ttccctttat gccttatatg
     8161 cttcaagtga agctattcaa catgctaatt taaatttaga tgaaattgat gcagatcgat
     8221 ttggtgtcat cgttgcatca ggtattggag ggattcaaga gattgaagaa caagtgattc
     8281 gtcttcatga aaaagggcca aaacgcgtaa aaccaatgac gcttccaaaa gctctaccaa
     8341 atatggcagc tggaaatgtt gcgatgaggt taggcgctca tggtgtatgt aaatcaatta
     8401 atacagcttg tgcttcttct aatgatgcta ttggagatgc tttccgtaat ataaaatttg
     8461 gtattcaaga tattatggta gtcggtggtg cagaagctgc tattacaaaa tttgccattg
     8521 ctggattcca aagtcttaca gcattatcaa caactgaaga tccatcaaga gcatctattc
     8581 catttgataa agatcgcaat ggatttatca tgggagaagg ttctggaatg ttggtcttag
     8641 aaagtttaga acacgctgaa aaacgaggag ctactattct tgcagaagtt gttggttatg
     8701 gtaacacttg tgacgcatat catatgactt caccacatcc agaaggttta ggagccacta
     8761 aagctattca acttgcactg gttgaagcta atattaaacc tgaagaagtt aactatgtaa
     8821 atgctcatgg aacttcaaca cctgctaacg aaaaaggaga gagtcaagct attgttgctg
     8881 cacttgggac agacgttcca gtttcttcga ctaaatcttt tactggtcat cttttgggtg
     8941 ccgcgggagc tgttgaggca attgcaacta ttgaagcgat acgtcatagt tatgttccaa
     9001 tgactgctgg aacgacagaa ttatcagaag atattacagc gaatgttatt tttggtcaag
     9061 ggcaagacgc tgatattcgt tatgccattt caaatacatt tggttttgga ggacacaatg
     9121 cagttctggc cttcaaacgc tgggaggatt aaattggata ttcaagagat taaggattta
     9181 atgactcaat ttgatgagtc aagtctgaga gaattttcat tcaaaacaag tgacggtgag
     9241 ctgtctttta gtaaaaatga gggaaaagct ccacttgtcc caactatgtc tcctatgagt
     9301 catcaacctg aagcgactcc aactatagca actcctgtct ctaatgaagc aggtgagcaa
     9361 acgaaacaag ctactgaagt agtttcagaa atcccagagt cgactgtgac tgttgcagaa
     9421 ggtgacgttg tcgaaagtcc tttagttggg gttgcttatt tagcttctgg accagataaa
     9481 cctaactttg tttcagtagg tgatagcgtg aagaagggac aaactttgat gattattgag
     9541 gcaatgaagg tgatgaatga agtgcctgcc ccccatgatg gtgtagttac tgaaattctt
     9601 gtagccaatg aagaagttat tgaatttggt aaaggattgg tacgaatcaa atgattgata
     9661 taaaagaaat tcgtgaggct ttacctcacc gctatccaat gttattagtg gatcgtgttt
     9721 tagaggtatc agaagatgag atagtcgcta tcaaaaatgt cagtatcaat gagccttttt
     9781 tcaatggtca ttttccagaa tatcctgtaa tgccaggcgt tttaattatg gaagctttgg
     9841 ctcaaactgc aggtgtttta gaattatcta aagaagaaaa caaaggaaag cttgtttttt
     9901 atgcaggtat ggataaagtg aaatttaaaa agcaagttgt tccaggagat caattagtta
     9961 tgactgctaa gtttgtcaaa cgtagaggaa ctatagccgt tgttgaagca atagcagaag
    10021 ttgatgggaa attagctgcc agtggaacgt taacatttgc aataggcaat tgaagatagt
    10081 attgaattta gagggagaga agcatcaaaa atgtttaaga aaatattgat tgctaatcgc
    10141 ggagaaattg cagtacgtat cattcgtgca gcccgtgaaa tgggaatttc gacagttgct
    10201 atctattcgg aggctgacaa ggaatcattg cacacaattt tagcagatga agctatttgt
    10261 gttggaccag ccaaatcagc agaatcttat ttaaacgtta atgctatatt atctgctgct
    10321 attgtgactg gagcggaagc tgttcatcca ggttttgggt ttttgagtga aaattctaaa
    10381 tttgctacta tgtgtgaaga gatgaactta aaattcattg gtccatctgg agaagttatg
    10441 gataaaatgg gtgataaaat caatgctcgt acagagatga taaaagctga tgttccggtt
    10501 atcccaggat cagatggtca agttacaagt gtagaagaag ctgtatcaat tgctgaggaa
    10561 atcggctatc cattgatgtt aaaagcctca gccggtggcg gtggaaaagg aattaggaag
    10621 gtcaaatcag cagatgaatt gaagcctgct tttgagtctg cttcacagga ggctttggct
    10681 gcgttcggta atggtgctat gtatattgaa aaagttatat acccagctag acatattgaa
    10741 gtacaaatac ttggagatag ctttggtaag atagttcacc taggagaacg tgattgttct
    10801 ctgcagcgta acaatcagaa ggtattggaa gaaagtccgt ctgtggcaat aggtaacact
    10861 ctgcgtcaac aaattggtga agcagctgtt cgtgcagctg aggctgtatc ttatgaaaat
    10921 gcaggaacaa ttgagttttt gttagatgag aatagcggac agttctattt catggaaatg
    10981 aatactcgtg ttcaagtaga acaccctgtt acagaatttg taacgggtgt tgacatcgtt
    11041 aaagagcaga ttcgtatcgc tgctggaatt cctttatcag tttctcaaaa tgatattaag
    11101 ttaactggtc atgcgattga atgtcgtatt aacgcagaga atccacaatt taattttgcg
    11161 ccatgtccag gtaccattaa tgggttacat ctacctgctg gcggtatggg cttacgtgtc
    11221 gattcagcgg tttacacagg ctacactatt cctccttatt acgatagtat gattgcgaaa
    11281 gtaattgttc acggagaaaa tcgttttgat gcacttatga aaatgcaacg tgccttatat
    11341 gagcttgaaa ttgatggtat tgtaacaaat acagaatttc aaatggatct catttctgat
    11401 aaaaaagtct tggctgggga ttatgatact tcattcctga tggaagactt tttaccaagg
    11461 tattatggta acaatcaata ggagggaaga tggctttatt tagtaaaaag gataaatata
    11521 tccgtattag tccaaacaaa gcattagggt cttctgataa acgtagtctt cctgaggttc
    11581 ctgatgaact ctttgctaag tgtccttctt gtaaacatat gatttatcag aaagatttag
    11641 gattagcaaa gatttgtcca gcttgttctt acaactttag aatttcagcg caagaacgtc
    11701 tgttgctaac agttgacgaa gatagttttg aggaattatt tacaggaatt gaaactaagg
    11761 atcctttgaa ttttccaaac taccgtgaaa aactggctgc tacacgtcaa aagactaact
    11821 tagatgaagc ggtggtgaca ggtttagcta aaataaaagg acaaactacg gccttagcta
    11881 ttatggattc tcattttata atggcgtcta tgggaacggt cgtaggcgaa aagttgacaa
    11941 gattatttga gctagcaact gaaaagaaat tacctattgt tatttttaca gcgtcagggg
    12001 gcgccagaat gcaagaaggt attatgagtt tgatgcaaat ggcaaaagta tcggcagcag
    12061 ttaaacgcca ttctaaccag ggattgtttt atttaacaat tttaacagac ccaacaaccg
    12121 gcggcgttac tgcgagtttt gctatggaag gtgatattat tctggctgag ccacaagcgt
    12181 tggttggttt tgctggtcgt cgtgttatcg aaacaacagt gagagaagat ttacctgaag
    12241 gctttcaaaa ggcagagttt ttgttagaac atggttttgt cgatgctatt ataaatcgaa
    12301 cagaattacg cgactgcatt gctcaactaa ttgctttcca tggaaatgag gttggtcatg
    12361 aataatgatg tgacaagaat tttaaaagat gcgcgtgatc aagggaggct aactgctctt
    12421 gactacgctg aattgatttt tgataatttt atggaacttc atggtgatcg ccagttcgca
    12481 gatgacaaat caattatagg aggtttgggg tacttggcag ggcgtcctgt gacgattgtc
    12541 ggtattcaaa aaggtaagaa ccttcaagat aatttagacc gtcattttgg tcaaccacat
    12601 cctgaaggtt atcgtaaggc tttacgttta atgaaacaag ctgagaagtt tggaagacct
    12661 gttataacct tcatcaatac tgctggagca tatcctgggg taggtgctga ggagcgaggt
    12721 cagggtgaag caattgctag aaacctctta gaaatgagtg atttaaaagt ccctattata
    12781 gctattatta ttggtgaagg gggttctgga ggagcattag ctttagctgt tgccgataag
    12841 gtttggatgt tggaacatac tgtttattct attttaagtc ctgagggctt tgcctctatt
    12901 ttatggaaag atggtacaag aacaacagaa gcagcacaat taatgaagat gactgctgga
    12961 gaactttacc atatggaagt tgtagacaaa gtaatccctg aacatggtta tttttcaagt
    13021 gagattgtcg atatgattaa gacaagttta atttcagagt tagaagtgct ctcacaatta
    13081 tctcttgagg atttattgga acaacgttac caacgtttta gaaaatatta atagaaaaag
    13141 gctgagttat ttactcagcc tatatttagt ttttcaaggt attatcatag ttatagtttc
    13201 atattttgtt ataattgata a
//
LOCUS       DATRSD010000061        12791 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRSD010000061 DATRSD010000000
VERSION     DATRSD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.062"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            42..746
                     /locus_tag="VBD85_001455"
     CDS             42..746
                     /locus_tag="VBD85_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO7134004.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     YSLAKKYLQEKEFSQNRVIQTYRKAYQTFKTKKGARSSIEALLKRVNSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFSGDLKLTITNGGDEFYLIGEDFNRPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELINNRREIIFKEALDFIATNTNRFLNA
                     KTQTIILDKEHSEITL"
     gene            1013..2245
                     /gene="arcA"
                     /locus_tag="VBD85_001456"
     CDS             1013..2245
                     /gene="arcA"
                     /locus_tag="VBD85_001456"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO7134005.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLAVLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            2251..2682
                     /locus_tag="VBD85_001457"
     CDS             2251..2682
                     /locus_tag="VBD85_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134006.1"
                     /translation="MKLLVNHLPYQRAASLYIRFSVFVIERNIKMEEEFDDNDEQDTI
                     YAVLYDGKQPVSTGRFLPETQTEARLTRIATLKGYRGNGYGTKIIIALENYAKENGYH
                     YLTIHAELTAKDFYQTLGYQAIGNIYMEDGESCQTLEKYLI"
     gene            2698..3711
                     /gene="argF"
                     /locus_tag="VBD85_001458"
     CDS             2698..3711
                     /gene="argF"
                     /locus_tag="VBD85_001458"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7134007.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMDLIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3774..5201
                     /gene="arcD"
                     /locus_tag="VBD85_001459"
     CDS             3774..5201
                     /gene="arcD"
                     /locus_tag="VBD85_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO7134008.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTIVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSHPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKATPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVGYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            5222..6178
                     /gene="arcC"
                     /locus_tag="VBD85_001460"
     CDS             5222..6178
                     /gene="arcC"
                     /locus_tag="VBD85_001460"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7134009.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIIAN"
     gene            complement(6299..7324)
                     /gene="trpS"
                     /locus_tag="VBD85_001461"
     CDS             complement(6299..7324)
                     /gene="trpS"
                     /locus_tag="VBD85_001461"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO7134010.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(7432..8124)
                     /locus_tag="VBD85_001462"
     CDS             complement(7432..8124)
                     /locus_tag="VBD85_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7134011.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
     gene            8368..9240
                     /locus_tag="VBD85_001463"
     CDS             8368..9240
                     /locus_tag="VBD85_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7134012.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            9306..10925
                     /locus_tag="VBD85_001464"
     CDS             9306..10925
                     /locus_tag="VBD85_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7134013.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            11048..>12791
                     /locus_tag="VBD85_001465"
     CDS             11048..>12791
                     /locus_tag="VBD85_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7134014.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DGFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFLISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEVPRRTRLAYGFLLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILV
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYLFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQ"
BASE COUNT         4146 a         2191 c         2221 g         4233 t
ORIGIN      
        1 gtaattcttc tgatatcttt taaaaaaatt gagaggcctt catgactaaa tttattgttg
       61 attcatcgta ctggaatttg tttccaactt ctaagattgg ggtaatctta atcaaagatt
      121 atcacatgga caggaacctt gaaacagaac ttaaacagtt actgtctgat agttattctt
      181 tagcaaaaaa atatttgcaa gagaaagaat tcagtcaaaa cagagttatc caaacttatc
      241 gcaaagcata tcaaaccttt aaaactaaaa aaggtgcacg ttcaagtatc gaagcattat
      301 tgaagcgagt taattcagga aacgaaatta ctagtataaa tcctctagtt gatatctata
      361 atgccgctag ccttaggttt ggattaccta ttggtgctga agatagtgat acatttagcg
      421 gtgatttaaa actaactatt accaatggtg gtgatgaatt ttacttgatt ggcgaagatt
      481 ttaacagacc aactctttca ggtgagcttg cctatgttga tgatgttgga gcagtttgcc
      541 gctgttttaa ttggcgtgat ggaaaacgta ctatgattac cgataatact cagaacgctt
      601 ttttagttat cgaattaatt aataatagaa gagaaattat ttttaaagaa gcgttggact
      661 ttatcgccac caatacaaat cgctttctaa atgctaaaac acagaccatc atattagata
      721 aagaacacag tgaaataacg ctataacatt aaaacgaaaa gtaaaaattt tacttttcgt
      781 tattttttta ggttaataat ttctcacttt ccaacgataa aagaaagaaa ttgtatagaa
      841 atcgctttca tttttataaa tttaaatttt tttggatata atcgtgacat tcttaacact
      901 tttaatgcga ataacttccc tattttatat tcaaaagaaa gtagaatgta agtgtagata
      961 tagagataaa tcctgtttca atgcaaatct ctaactaaga aaggagtaaa atatgactca
     1021 aacacatcca attcatgttt tctcagaaat tggaaaactt aaaaaagtta tgctacaccg
     1081 tccaggtaaa gaaatagaaa atctgatgcc tgattattta gagagactct tgtttgatga
     1141 tatcccattt ttggaagatg cacaaaaaga acacgatgcg tttgcacaag cacttcgcaa
     1201 cgaaggagtc gaagtacttt accttgaaaa tcttgctgcc gagtcgttaa caaaccaaga
     1261 aattcgtgaa cagtttatcg atgagtacat tggcgaagca aatgttcgcg gtcgtgctac
     1321 taaaaaagct attcgagaat tattactcaa tatcaaagat aataaagaac ttatcgaaaa
     1381 aactatggca ggtatccaaa agtctgagct acctgaaatc ccatcttctg aaaaaggtct
     1441 aactgatctt gtcgaatcta attatccatt tgccatagat ccgatgccaa atctttactt
     1501 tacacgtgat ccatttgcaa ctattgggaa tggagtttca ttaaaccata tgttctctga
     1561 aacacgtaat cgcgaaactt tatacggtaa atatatcttt acacatcacc cagagtacgg
     1621 tggaaaagtc cctatggtct atgatcgtga agaaacaact cgtattgaag gtggagatga
     1681 gctcgtcctt tctaaagacg tccttgccgt gggtatctct caacgtactg atgctgcctc
     1741 tattgaaaaa ctcttagtca atatttttaa acaaaattta ggttttaaaa aggttttagc
     1801 atttgaattt gcaaataatc gtaaatttat gcaccttgac acagtcttta caatggttga
     1861 ctatgacaag tttacaattc acccagaaat tgaaggcgat cttcgcgttt actctgtaac
     1921 ttacgaaaac caagatcttc atattgaaga agaaaaaggt gatttggcag ttcttctagc
     1981 gaaaaaccta ggagttgaga aagtggaatt gattcgttgc gggggtgaca acttagtcgc
     2041 tgctggacgt gaacaatgga atgatggttc aaatacatta actatagcac caggtgtcgt
     2101 tgtcgtttac aacagaaata caatcacaaa tgcaatctta gagtccaaag gattgaaatt
     2161 aattaagatt aatggaagtg aattagttcg tgggcgtggt ggaccacgct gcatgtcaat
     2221 gccatttgaa cgtgaagacc tttaaaattt atgaagctat tagtcaatca cttgccttac
     2281 caaagagctg caagcctata tattcgcttt tctgttttcg ttatcgaaag aaatataaaa
     2341 atggaagaag aattcgatga taatgacgag caagatacta tttatgctgt tctttatgat
     2401 gggaaacagc cagtttcaac aggacgcttt cttcctgaaa ctcaaacaga agctcgtttg
     2461 actcgaattg cgactttaaa aggttatcgt ggtaacggct atggtaccaa aattattata
     2521 gcactagaaa attatgcaaa agaaaatggt tatcactatc taactataca tgctgaattg
     2581 acagcaaaag atttctacca gacacttggg tatcaagcta ttgggaatat ttatatggaa
     2641 gacggggaat cctgtcaaac actcgaaaaa tatttaattt aaaggagaca ttaacatatg
     2701 acacaagtat ttcaaggacg tagtttctta gcagaaaaag atttttctcg tgaggaattt
     2761 gaatatctta ttgatttttc agctcattta aaagacctta aaaaacgtgg tgttcctcat
     2821 cactatcttg aaggtaaaaa tattgctctc ttatttgaaa aaacatctac tcgtactcgc
     2881 gcagccttta caactgcagc aattgaccta ggcgctcatc cggaatacct tggtgcaaat
     2941 gatattcaac ttggcaaaaa agaatcaaca gaagatactg ctaaggtttt agggcgtatg
     3001 tttgatggta ttgaattccg tggttttagc caaagaatgg ttgaagagct tgctgaattt
     3061 tctggagtac ctgtttggaa tggtttaaca gatgaatggc atccaacaca aatgctagct
     3121 gactacctta ctatcaaaga aaacttcggt aaacttgaag gtattactct tgtttactgt
     3181 ggtgacggac gtaacaacgt tgccaactca cttttagtgg ctgggacttt gatgggggtc
     3241 aatgtacaca tcttttctcc aaaagaactt ttccctgctg aagagattgt taaattggct
     3301 gaaggatatg ccaaagaatc tggggctcac gttctcgtta ctgataatgt agacgaagct
     3361 gtaaagggag cagatgtctt ttacactgat gtctgggtat cgatgggaga agaagataag
     3421 ttcaaagaac gcgttgaact tcttcaacca tatcaagtaa acatggatct gattaaaaaa
     3481 gctaataatg ataatcttat cttcttacac tgcttacctg cattccatga tacaaatacc
     3541 gtttacggca aagacgtcgc tgaaaaattt ggcgtcaagg aaatggaagt tactgatgaa
     3601 gtcttccgta gcaaatatgc ccgtcatttc gaccaagctg aaaatcgtat gcacactatt
     3661 aaagctgtaa tggctgcaac ccttggaaat cttttcattc caaaagttta aattaaacga
     3721 ctatagggag ttgacatata gtcaattccc tattttagga aatgaggtat aaaatggaaa
     3781 aagaaaaaaa attaggtctt ttaccactaa caatgcttgt cattggctct cttatcggtg
     3841 gcggaatctt tgatttaatg caaaatatga gttccagagc cggtttggta ccaatgctta
     3901 ttgcttgggt aattactgct atcgggatgg gaactttcgt tttaagtttt caaaatttat
     3961 ctgaaaaaag gccggaccta acagctggaa tctttagtta cgctaaagag gggtttggaa
     4021 actttatggg atttaactct gcatggggtt attggttatc agcttggctt ggaaacgttg
     4081 cctacgctgc actcttattc agttcactcg gttatttctt taaattcttt ggtaatggaa
     4141 ataatatcat ttcaattatt ggagcaagta tagttatttg ggttgtccat ttcttaattt
     4201 taagaggtgt taatacagct gcatttatta ataccatagt tacctttgca aaattagtac
     4261 ctgttattat tttcttaatt tcagcgttat tagctttcaa atttaacatt tttagtcttg
     4321 atatctgggg aaatggatta catcaatcaa tttttaacca agtcaattca actatgaaaa
     4381 ccgctgtttg ggtatttatt ggtattgagg gcgccgttgt cttctcaggt cgtgctaaaa
     4441 aacactctga tattggtaaa gcaagtatcc tagcattatt cactatgatt tcactttatg
     4501 tattgatttc tgttttatca cttggtatca tgtcacatcc agaacttgca aacttaaaaa
     4561 caccagctat ggcttacgtt ctagaaaagg ctgttggtca ctggggcgct atcttagtta
     4621 accttggtgt tatcatttca gtatttggcg ctattcttgc ttggacttta tttgcagcag
     4681 aattaccata tcaagctgct aaagaaggtg cttttcctaa attctttgca aaagaaaata
     4741 aaaacaaagc tccaatcaac tcactcttag tcactaatct ttgtgtacaa gcattcttaa
     4801 tcacgttctt attcacacaa agtgcttatc gttttggttt cgcattagca tcatctgcta
     4861 tcttaattcc ttatgctttt acagcactat atcaattaca attcacactc cgtgaggata
     4921 aggcaactcc aggacatcaa aagaatttaa ttatcggtat cctcgctaca atctatgctg
     4981 tttaccttat ctatgctggt ggttttgatt acttactttt gacaatgatt gcttacactc
     5041 taggtatgat tctctatatt aaaatgagaa aagatgacaa gcttcctatc tttgtgggat
     5101 atgaaaaaat tagtgccatt gtcattctag cgctttgctt actatgtatt attgaaatta
     5161 tgactggaca aattgacatt gctgcaatcc tagcaggtta gtcattagaa agagggataa
     5221 attggtaaaa gaacatcaaa aaatcgtagt tgccttaggt ggtaacgcta ttctatcaac
     5281 agatgcttca gcaaaagcac aacaagaagc attaatcaac acatcaaaat cactggtcaa
     5341 actgattaaa gaaggccatg atgtcattgt tactcatgga aatggtcccc aagttggtaa
     5401 cttattgcta cagcaagcag ctgcagattc tgaaaaaaat ccagcaatgc ctttggatac
     5461 ttgtgttgcc atgacagaag gaagtattgg tttttggtta caaaatgctc ttaacaatga
     5521 attacaagaa caaggcattg acaaagaggt tgcaacagtg gtaactcaag ttattgttga
     5581 tgaaaaagat caagctttca ctaatccaac aaaacctatt ggcccattct tatctgaaga
     5641 agatgccaag aaacaagccc aagaaacagg ttcaaaattt aaagaagatg ctggccgtgg
     5701 atggcgtaaa gtcgttcctt caccaaaacc ggttggtatc aaagaagctt ctgttattag
     5761 acggttagtg gactctggtg tggttgttat tagcgcaggt ggcggaggtg taccagttat
     5821 tgaagatgct aatacaaagg cattaaaagg ggttgaagct gttattgaca aagactttgc
     5881 cagtcaaact ttatcagaac ttgttgatgc cgacttattc atcgtcttaa ctggcgttga
     5941 caacgttttt gttaatttta ataaacctaa tcaagaaaaa ttagaagaag ttactgtttc
     6001 acaaatgaaa caatatatca ctgaaaatca atttgcccct ggcagtatgc taccaaaggt
     6061 agaagctgcg attgcttttg ttgaaaataa accagaagcg cgtgctatca ttacttcact
     6121 tgaaaatatt gataatgtct tagcagaaaa tgcagggaca caaattattg caaattaaac
     6181 aatgtagatt accattacat acactcctta ttaataacaa agtaatgtca ctccttaggc
     6241 attcctataa taaaagagag gtagatatat ctagctctct tttattatag gacatatatt
     6301 atttaaaata gttcaaaccc atggctgatt taacctcatc caaagtagag gctgcaacag
     6361 cttgagcttt ctcactacct ttttgtaaca tttgataaac ttgtcccata tctttagcat
     6421 attcaaggcg acgttctcta ataggtgata attcacgctc taagatatca agtagataac
     6481 gttttgtttt aacatctcca agtccacctt tttgatagtg ttctttcata gcagtaatct
     6541 ctttttggtc ttcatctctt ccaaaaacat caaggtaatg gaataccata ttaccttcga
     6601 tttgtcctgg ttcttctact ttaatatggt ttggatcagt atacatgctc ataacttttt
     6661 tcttaactgt atccatatca tctgctaaaa agattccatt atttaatgac ttagacattt
     6721 ttgcattacc atctaaccct ggcagacgac ctgctgcatc attttctgga tagattcctt
     6781 ctggttcaac taaaacctga caattataag catgattgaa agatctaact atttcacgtg
     6841 tttgttcaat cattggtttt tggtcagtcc ctacaggaac aaggttagct ttaaaagcgg
     6901 ttatatcagc tgcttgtgca acaggataaa ctaagaatcc tgctggaata gactcaccaa
     6961 aacctttttg agcaatttct gtttttacag ttggattacg ttccaaacgt gcaagtgaaa
     7021 ccaagttcat ataatacata cttaattctg ctaactcagg aatttgactt tgaatgaata
     7081 aagtcgactt gtttggatct agcccaacag ctaaataatc aagtgcaaca ttaccaattg
     7141 attcaacaat agtttgcgga tctttagcat ggtctgtcag tgcttgttgg tctgctaaaa
     7201 agacaaataa agtatagcta ccctcatttt gaagtagaac tctatttttt aagctaccta
     7261 cataatgtcc aatatgcaac tttcctgtcg gtctatcacc tgttaagata attggttttg
     7321 tcatgtttac tccttaatat accaaagata tcatttcata tagctatctt atcatgtcac
     7381 aagccctatt gcaattcatc cctacaagaa acattcacct tattaagatg attaaacatt
     7441 atgtaacgtt aaatcgttct taataatttc aatcgtataa tgaatcataa taattaaaag
     7501 cgttatcgtc acacctaaaa tagctctata tgcaaagata tcgtaaacta ttgcagctac
     7561 caatgctcca ataataaatg ctagaactac cagtgaataa ttgatagctc gtcttttttg
     7621 catagccgtt ctctgacgat taacaatata ctcataccaa aattgtagca ttttccgata
     7681 attaccagaa gtcattaaga tagtatatgg ttcagattca atgcgacttc ctgaaaatgt
     7741 caacattaaa atacctgtgc taaaggctaa taaagctacc cagatattgt ggtttacatt
     7801 ttcaacaaaa ggaataaaca tcgtagaaag taaaagggga cataatgcat atagacgcca
     7861 aaaagctgtt actgcacgtt ctttaatcac aataccaacc aaaaaaccaa ttgaaaaaaa
     7921 gaaaatagaa aataatttag tgataaaaaa gtgaaaatca ttttgtccga gatttgccac
     7981 catcaagata atattacctg tctgtgttgc aacaagggag tgaaactgca tgtgactata
     8041 aacgtcaata aaaccaccaa taaaacccaa taatacagcc atttttcttc catcttgtgg
     8101 cattataata tcatctctgc gcattatagc aaattctcct tcttgtatta tcatgttttc
     8161 tattatatac caataaaata ggcattcaac tcaaaaatat cgagaaattt cttttctaaa
     8221 tttacttgac taccaagtct cgctgttaga taataggtaa taataagttt tgataaaata
     8281 aaaatgttat gactgagttg tcaatattat ttattgacag ctttctcttt ttatcatgtt
     8341 attattacta aagttaagga gcttatcatg tctaaaaaaa ttaagaattt tatactatta
     8401 aatattggat ctctaattgt tgcatttggt ttcaacgctt tctttcttca taatcatatt
     8461 gtcacggggg ggattagtgg tttggcagtt agttttcatg aagtatttgg ttgggattct
     8521 gcttttttta ttatgattat caatattcca ttgctccttc tttgctactt tggcttaggt
     8581 aaacaaacct ttttaaaaac tgtctatggt tcttggattt ttcctgtttt tattaagtta
     8641 acacaaagtg taccaacttt gacccacaac ccactcctcg cagcactttt tggaggtgtt
     8701 attgtaggat gtggtttggg gattgttttt tggagcgact cttcaactgg tggaacgggg
     8761 attatcattc aattcttagg aaaatatact cctataagcc ttggacaagg ggttatattg
     8821 attgatggac ttgttacaat tgttggtttc ctagcttttg acagtgatac ggttatgttt
     8881 tctattattg ggttgataac tattagttat attattaatg ctatccaaac tggttttaca
     8941 accttaagca ctgtcttaat cgtttctcaa gagcaccaaa aaattaagac atatatcaat
     9001 actgtcgcag atagaggagt aacagaaatt cccgttaaag ggggatattc tggaactaat
     9061 caaatcatgc ttatgacaac tattgctggt tatgagtttg ctaaattaca agaggcaata
     9121 gcagaaattg acgaaacagc cttcataaca gtaactccaa catcacaagc ttctggacgt
     9181 ggatttagtc ttcaaaaaaa tcatggacgt cttgatgaag acattcttat gccaatgtaa
     9241 tcgattgata attaaagctt tttctagtag aatagcagat agaatataca caagaggtaa
     9301 caaaattgct tacagtttca gatgtttcac tacgttttag tgatcgcaaa ttatttgatg
     9361 aagttaacat taactttaca gcaggaaata cctatggtct tatcggagca aatggcgctg
     9421 gtaaatcaac tttccttaaa attttagctg gtgatattga accaacaaca ggacatattg
     9481 cacttggccc agatgaacgc ctttctgtac ttcgccaaaa tcattttgat tatgaagatg
     9541 agcgcgtcat tgatgtcgtt atcatgggta atgaaacact ttatagtatc atgaaagaaa
     9601 aggatgctat ctacatgaaa gaagattttt ctgatgaaga tggtgttcgc gctgcggaat
     9661 tagaaggaga atttgctgaa ctcggtggat gggaggctga aagtgaggct tctcaattac
     9721 tccaaaatct aaatatttca gaagagcttc attatcaaaa tatgagtgaa ctagctaatg
     9781 gagacaaagt taaagttctc cttgcaaaag ctctctttgg taaaccagat gtacttctcc
     9841 ttgacgagcc taccaatgga cttgatatcc agtctattac ttggttagaa gatttcttga
     9901 ttgactttga aaatacagtt attgtagttt cccacgaccg tcatttcttg aataaagtat
     9961 gtacacatat ggctgacctt gattttggaa aaattaaact gttcgtcggt aactatgatt
    10021 tctggaaaga atcatcagaa ttagcagctc gtttacaagc tgatcgtaat gctaaagcag
    10081 aagaaaaaat taaacaactt caagaattcg ttgcacgatt ttcagcgaat gcttcaaaat
    10141 caaaacaagc aacttctcgt aaaaaaatgc ttgataaaat tgaacttgaa gagattgtac
    10201 catcaagccg taaataccca ttcgttaact ttaaagccga acgcgaaatg ggaaatgatt
    10261 tattgaccgt tgaaaatctc tctgtcacta ttgatgggga aaaaatcctt gataatatca
    10321 gtttcattct tcgtcctggt gataaaactg cacttattgg acaaaatgat atccaaacta
    10381 ctgcacttat tcgagcattg atgggggata ttgaatatga aggaacaatc aaatggggag
    10441 taacaactag tcgttcttac cttccaaaag acaattcacg agactttgct tctggcgagt
    10501 caatcttaga atggcttcgt caatttgcta gtaaagaaga agacgataat accttcttac
    10561 gtggtttcct aggtcgtatg cttttctctg gagatgaggt taacaaatct gttaatgtct
    10621 tatctggggg tgaaaaagta cgcgttatgc tttcgaaatt aatgctatta aaatctaatg
    10681 ttcttgttct tgatgatcca acaaaccact tagatttgga atctatttca agtctcaacg
    10741 atggactaaa agatttcaaa gaatctatta tttttgcaag tcatgaccac gagtttattc
    10801 aaactttagc gaaccatatt attgttcttt ctaaaaacgg agttatcgat cgtatcgatg
    10861 aaacttacga tgaatttctt gaaaataccg aagtacaagc taaggtcgct caactctgga
    10921 aataatcctt tggcatatct ctaacctaat aaacgtgtca agattgaaaa tcttaaaact
    10981 atattaatca tttaaataaa aaggttggaa caaatagtgt ccagcctttt tatcttagga
    11041 gatatatatg acaattaacc actataagct tcgtatacct tactatacaa taagctttct
    11101 acttcccttc ataatcatag tctgtatcct ctttactaag aatatttatt ggggaagtcc
    11161 aacaaccata ttagcaagcg atggctttca tcaatatgtt atctttaatc aagcattaag
    11221 aaatattctt catggttcaa acagcttatt ctataccttt acaagcggtt taggtttaaa
    11281 tttttatgct ctgagtagtt actatttggg aagtttttta tccccaatag tttatttctt
    11341 taacctaaaa aatatgcctg atgctatcta ccttttaaca atatgtaaaa tagggttgat
    11401 aggtttgtca atgtttgtca ctctttgtaa acgtcattgt aaagttaatc gtgtcttact
    11461 tttagtaatt tctacttgct atagcttaat gagtttctta attagccaaa ttgaaattaa
    11521 tatgtggcta gatgtattta ttctaattcc ccttgttgtt ttaggagttg atcagctact
    11581 atgggaaaga aaacctatac tttatttcct ctctctgaca gccttattca tccaaaacta
    11641 ttattttggt tttatgactg ccatctttac tagcctttac ttcatcgtac aaataacccg
    11701 taatactgat agtaaggttg catttaaaca atttcttcat tttacctttt tatctctttt
    11761 agctggtatg actagtagca ttatgatttt gccaacttat tttgatttaa ctactcatgg
    11821 tgaaaagttg acaaaggtat cgaagatgtt tacagaaaat tcttggtaca tggatttgtt
    11881 tgctaaaaat atgattggag cctatgatac aacaaaattt ggctctattc ctatgattta
    11941 tgtaggttta cttcctttac tcctttcttt actttacttt acaataaagg aagttcctcg
    12001 aaggactaga ttagcatatg gatttttact aatatttgtt attgctagtt tttatataac
    12061 acctctagat ttattttggc aagggatgca tgctccaaat atgtttctcc atcgctactc
    12121 ttgggtactt tcagtactta tctgtctgct agctgctgaa tgccttgagt atttagacaa
    12181 catttcttgg aaaaagattt taggggtcaa tttaatttta gtaagtggtt tcataatcac
    12241 gtttttattt aaaaagcatt atcattacct taatcttgaa ttacttcttt taaccttaac
    12301 tttcctctca gcttatatta ttctaactat tagttttgtc agtaagcaaa tacctaaact
    12361 agttttttat ctctttctta ttggttttgt tgtgttagag atgactttaa acacttttta
    12421 ccaactaaat agtcttaacg atgagtgggt atttccatct aggcaaggat atgctaaata
    12481 taatcacagc attagcaaac ttgtcagaaa gactgagaga aataactcta cattcttccg
    12541 tacagagcgc tggttaggac aaaccggcaa cgactctatg aaatataatt ataatggtat
    12601 ttcacaattt tcatctatta gaaatcgttc ttcaagtcaa gtgcttgata gacttggctt
    12661 caaatcagat ggtacaaatt taaatttacg ttatcaaaat aatactttga tcgcagatag
    12721 tctatttggt gtaaagtata accttacaga atatcctttt gataaatttg gttttatcag
    12781 aaaagctcaa g
//
LOCUS       DATRSD010000062        12316 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRSD010000062 DATRSD010000000
VERSION     DATRSD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12316
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.063"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(79..1170)
                     /gene="purK"
                     /locus_tag="VBD85_001466"
     CDS             complement(79..1170)
                     /gene="purK"
                     /locus_tag="VBD85_001466"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO7134015.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVVVAPYDDVEALGQLAARCDILTYEFENVDADGLDAVVSAGHLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGIDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LSEAQQLANSAQCVLEEFVNFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKEMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHAPAVMLNVLGQHVQQAIDYVAENPSAHLHMYGKLE
                     AKHNRKMGHVTVFSNTPDEVEEFEERMDF"
     gene            complement(1157..1645)
                     /gene="purE"
                     /locus_tag="VBD85_001467"
     CDS             complement(1157..1645)
                     /gene="purE"
                     /locus_tag="VBD85_001467"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO7134016.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARDRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(1931..3193)
                     /gene="purD"
                     /locus_tag="VBD85_001468"
     CDS             complement(1931..3193)
                     /gene="purD"
                     /locus_tag="VBD85_001468"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO7134017.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLGL
                     VNIGISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKLAENSKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            3476..4279
                     /locus_tag="VBD85_001469"
     CDS             3476..4279
                     /locus_tag="VBD85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO7134018.1"
                     /translation="MTIYDQIESALDLMTDLEREIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(4302..5279)
                     /locus_tag="VBD85_001470"
     CDS             complement(4302..5279)
                     /locus_tag="VBD85_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7134019.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNDSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIVATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     TQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(5287..6168)
                     /locus_tag="VBD85_001471"
     CDS             complement(5287..6168)
                     /locus_tag="VBD85_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7134020.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEISRWDGRRIFQEAKKGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTTIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(6185..7102)
                     /locus_tag="VBD85_001472"
     CDS             complement(6185..7102)
                     /locus_tag="VBD85_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO7134021.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAIGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(7099..7761)
                     /locus_tag="VBD85_001473"
     CDS             complement(7099..7761)
                     /locus_tag="VBD85_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO7134022.1"
                     /translation="MKKANQLIAAIFDVNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVSFLKITCYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(7781..8224)
                     /locus_tag="VBD85_001474"
     CDS             complement(7781..8224)
                     /locus_tag="VBD85_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7134023.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGHEVIKLGKGDKVEVEEYLGDIGFIK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLDNYVKKGVLKVLMA"
     gene            complement(8237..9067)
                     /locus_tag="VBD85_001475"
     CDS             complement(8237..9067)
                     /locus_tag="VBD85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7134024.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(9077..9964)
                     /locus_tag="VBD85_001476"
     CDS             complement(9077..9964)
                     /locus_tag="VBD85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7134025.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(10052..11368)
                     /locus_tag="VBD85_001477"
     CDS             complement(10052..11368)
                     /locus_tag="VBD85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134026.1"
                     /translation="MSIKKSVIGFCLGAATLSMFACVDSSQSVMASEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(11415..12113)
                     /locus_tag="VBD85_001478"
     CDS             complement(11415..12113)
                     /locus_tag="VBD85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO7134027.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLASLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPEQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
BASE COUNT         3778 a         2567 c         2140 g         3831 t
ORIGIN      
        1 atatccctgt ttattattct attcatctga caacactccc ttcaaaatca ttctccccct
       61 aaaattccat aaacctcctt agaaatccat cctttcttca aactcttcca cctcatcagg
      121 tgtattacta aacactgtca catgtcccat tttgcggttg tgtttcgctt ctagtttacc
      181 atacatgtgg aggtgggcgc tagggttttc ggcaacatag tcaattgcct gctggacatg
      241 ttgtccgagg acattgagca taacggctgg agcatgaagc ttgattggtg ggagaggttg
      301 ccccagtacg cccaagatgt gggtgtcaaa ctgtgaaaag tcgcaggctt cgatagagta
      361 gtgtcctgag ttgtgagggc gtggagcgat ttcgttgaca atgatgtcgt ctgcggtcgc
      421 aaacatttcc acacagagag ttcctgatag ctggagtttc ttggcaatct gcaccgccat
      481 ttctttagcc ttgtcagcta gttggtctga gatgcgagct ggtacgatgg tttttgacag
      541 gatattgttg cggtggatat tttcctgaac aggaaagacc gtcacatcct gaccatttcc
      601 agacacgatg actgagattt caaggtcgaa gttgacaaac tcttccaaga cacactgagc
      661 tgagttggct aattgctgcg cctctgacag gtcttctgct gagcggataa ccttttgccc
      721 atgaccgtca taaccacctg tcgctgtctt gaggacatag gtcttggtca agtcaatccc
      781 ctctagatca aggctagacg tcaccacctt atagggagcg acagtcacac cagccttatt
      841 tgccagaaag tctttttcaa agatacgatt ttgggaaatg cggagcagat cagtcccctg
      901 cggtagatga ccagctgaca caacggcatc cagaccgtcg gcatcgacat tctcaaactc
      961 ataggtcaaa atatcgcaac gcgcagccaa ttgtcccaaa gcctcaacat catcgtaagg
     1021 cgccacaacc acctcgctaa cgcgagaggc agggcagtcg ctagcaggat ccagcgtaat
     1081 gaccttgtgg cccatgtaga tagccgcaat cgccatcatc tgccccagct gaccaccacc
     1141 aataatccca atggtcttaa atgagttcat tgcttgactc ctctgcgatt tttccttgtt
     1201 cctcatggaa atgagctagc gcatcagcca aattttggtc ttcgatagag aggatacgga
     1261 gggctgtcag ggcagcgtta gtcgctcccg cttctccgat agccatggtt gccacaggca
     1321 caccgccagg catctgcaca atggaataga gcgaatccaa acctgaaaga gcacgtgatt
     1381 tgacaggcac accgataaca ggtagcgttg ttttagctgc caccatacca ggcaagtgag
     1441 ctgctccacc tgcaccagcg atgatgattt tgatgccacg gtcacgtgct tcctcagcat
     1501 gcttgaacat gaggtctggt gtgcggtggg cagagacgac tttcttctcg taagcaatgc
     1561 caaagttatc tagaacttca gccgtttttt gcatggttgt ccagtcagat ttggatccca
     1621 tgataataga aataattggt tgcataatat taagatacat agggacgttt cgacttgtcg
     1681 aaaattttcg tagaaaaata tgcggtaagt ccgaaatctt tgatttcgaa gacgcaccca
     1741 cgtcagggcg actaggtgct ttagcagcag acgcctacga cgcagaaatc tgacaaagat
     1801 gctttagcat ctaggaagat ttatcttttt cactaggaaa ttagtcccgt gttcagttcc
     1861 tttcttcttt ctttttggag cgaccttcgg tcgcttattg ctgacgctaa tatttgaatg
     1921 agtagtctta ttacttaata gctttgcttc cgatatcatt tcggtagaag aggcctgttg
     1981 tgtcttgttg ggctagttgg gtatagattt tgtcctgccc tgctttgacg ctgtcttctg
     2041 tggtgactag catatagaca cgtcctccgt ttgagagtag tgctttgcta ttttccgcaa
     2101 gcttagctcc tgcgtagtag gtgatgatgt cgccgtcggt cttctcaggc agtggcacac
     2161 ctttctcgta atcgagcgga tagccttctg atgcaacgac aacgccaaga gtcacgccgt
     2221 ccttctgcca agtgatgtaa ggctcaatgc ccatcatgat gtcgtcgatg ttctgagcga
     2281 aatcagaagt caggcgaggg aggataatct gagtttcagg gtcaccgaaa cgtgagttga
     2341 actcgataac cttagggcca tcagctgtca ggataagccc cgcatagagg acacctagat
     2401 aaggacgccc ttcggcaatc ataccctcca ggacaggttt aacgatcgtc tcaactgctg
     2461 tatccaccac gctctgaggc aggtgaggaa caggcgcata ggcacccata ccaccagtat
     2521 ttggcccctt gtctccgtca aaggcacgct tgtgatcctg agctgtcggc atgatgtaga
     2581 acttatcgcc attagcaaag gcaaagaggg aaaactcctc gccatccaag aattcctcga
     2641 taaccacacg cgctcctgag tcgccaaact tgttatctag aagcatctct tgcgccgcct
     2701 ctaccgcctg ctcaacggtt tcagccacga caacaccctt acctagcgcc aagccatcag
     2761 ccttgaccac gattggtgcg ccctgctctt cgatgtaggc tttagctttt tcaaaatctg
     2821 aaaatgtgcc ataggctgct gttgggacat tgtatttaac catgatttcc ttagcaaagt
     2881 cttttgacca ctctagctcc gctgctgcct ttgttggccc aaaagctcta agtccagcac
     2941 tgttgaaatc atcaacgata ccagccgcta gcgcatcatc aggaccaata aaagtccaag
     3001 caatctcatt ctccttagca aagtcaatca gcctagaatg ttcggaaatt ccgatattta
     3061 ccaagcctag accatccaag gtcataccat cattaccagg tgccacaaaa acctgatcta
     3121 cacccttaga cgctaacaac ttcttagcaa tcgcatgctc acgaccacca gaaccaacaa
     3181 caagcaattt catatcagaa taactcctat taaataatat aaaaagaaat ttgcaataaa
     3241 aacgtattat ttataaaatt ataacacatt tgttcgcttt taagtttttt tattgcatct
     3301 tttttgaaaa attaataaca taatatatac ttcacatata ttatgttatt aatccttcat
     3361 attgctattt aaaacttatc attgctgttt cttagaactt agtttggccc ctcgctaact
     3421 ttatgttata ctgaatatgt taagcgcttt cttaaagagg agaggaccgt gcattatgac
     3481 tatttatgac caaattgagt ctgctcttga tttaatgact gatttagaac gtgaaattgc
     3541 ttgttatttt atggggcagc ccatttcaaa agacgctctt gcttcttcca ttgtcactaa
     3601 gcaactacac atttcacaag cagccttaac gcgttttgct aaaaaatgtg ggtttaaagg
     3661 ctatcgtgaa tttgtttttg aatatttaaa aagccatgag accatcagtc aacaattata
     3721 cggtcttcaa aatgacaaca ctaagaaagt atttatgaat tatcaagaga tggtttctaa
     3781 atccgctgat attattgatg aagaacagct tctagaagta tcacatatga ttgagcaagc
     3841 tgatcgtgtt tatttttatg gcaaagggag ctcatcgcta gtagctaaag aatttaaaat
     3901 acgccttatg aggctgggag tgatttgtga ggctctggat gatacagata gtttctcatg
     3961 gactaatagt attgttaatg atagatgcct cgtcattgcc ttctcacttt ctgggaacac
     4021 caactctgtt atcggtgcct taaaaatcgc aagttgtcac ggagctaaaa ccgttctatt
     4081 tacaaaacaa cctcacacaa ttgactatgc ttttgacaag attattcagg tggcctctgc
     4141 taggcattta gactacggca atagaatttc gcctcagatc ccaatgttaa tcatggtaga
     4201 tattatctat gcacaatttt tagatattaa taaaatagaa aaagagcgaa tttttagaga
     4261 aaccattatt caacgctaaa tgaaaatcga gtagaaataa tctactcgat tttttgcttt
     4321 gtaaattgac ttcctgtcag ttgatcaaaa atatagtctt ttacttgcac tgtcattgga
     4381 tcatgtccat agtctggtaa taaaagatgc tttttagttg atgtcagacg attaaaaatg
     4441 gcaaattgag ttgatggagg acaaatatca tcttttaagg ctgttagtaa aacaacgggg
     4501 caagaaatcc tatgtgcaaa atttttaaca tcaatataag ccaaagtttt taacacatta
     4561 ttttctgttt tatgaaaagg atcactatat ttaaaatagc ggaagagttc atcataaggc
     4621 tcactgacat tccctaaatc taatactcgt ctaaagtctg acaaaaatgg gtaaacagca
     4681 acggtcttga cgattttggg attaagggct gcagctatca aagcaagtgc tccaccttga
     4741 gaccagccat aactataaag ttgattacta tcaacactct ctaatgtagc aacgatatca
     4801 atcaattgga aaacatcaag ataaatgtct ttataaaaaa gatgatttgg tcctgagatc
     4861 attcctcgca caatttggcc cttaactgta atgccatcaa aatgcccctt gtcttgagac
     4921 tgtccagctt gacctcgaac atccattgaa acaacgccat aacctgctgc tacataatta
     4981 agttggtcac tccagtcagg actttggttt tggtagccat gaaaataaaa aactacagga
     5041 tagggtttat ttgttttagg gaataaacat ttggcataga caatagagtc attgctacct
     5101 ttaaaatgaa gatcataagc ttgtgcaaaa gtaatattga aatctttttt gtctagacga
     5161 tactcaatat tcccttgata tttcattgtt tgttttttcc agaaatcatc aaaatcctcg
     5221 ggaatttggt cttgacctag gtattctctc atgtcatcta gcgacattgt ttcaatcata
     5281 tacccgtcac cccctgtttt taaaatggta gtaagctcct agcataccag cttggttttc
     5341 gtgttgtgca aaaactattg ttgttttttc agcaagactt gtcacaagat ttcgtttcaa
     5401 agattcacta agtttatctt gtaaataatc tttttgagcc atgattccac cacctaaaac
     5461 aactttttca ggatttacca cataaaccat attagcgatt ccttgaccaa ggtagtttat
     5521 catacggtct atacttgcaa tacatttttc attgcctttt ttagcctcct gaaaaatacg
     5581 acgtccatcc catcggctaa tttcatctcc atgcgctttg gcaacatcag caatcagtgc
     5641 agttgtagag gctaaatctt gaaaatcacc atctgaaagg tgcatatagc caacttcaca
     5701 agcagaatta ctaaagccat gaaaaactgt tttatcaata atcagacaac caccaatacc
     5761 tgttccaata gtaagacaaa gcgctattga actatctttg gcactacctg agacagcttc
     5821 agctaaccct gcacaattaa catcattttc aatttctgtg cgtacttgat aagtgtcttc
     5881 taaaactttc ttaaattgtg tcccagcata gtttggaatt tgaggaccag agtaaaatat
     5941 gcatccttca tctggatcta ccatgccagc agatgagata gcaattccat caatgctccc
     6001 ttctgttaga taattatcga taatttgaca aactttttgt aaaatgcctg gaccaccttt
     6061 gtaggcttct gtggcaagct cacttgcctc aacaatacaa cctaaattat ccacaatgcc
     6121 atgcttaatc attgtaccgc caatatcaat tgctactgtt ctggtcataa ttatctacct
     6181 cactttaaca aaactgtttt ttagcatgtc gaatcatttc tgctgcctct ttaataatag
     6241 gtttatcttc ttctgttaca gatgctaaag gtaaccgaac ggaacctata gttagttgct
     6301 cattgatttc caatacagct ttgatcacag catacatatt accatgacct gaacaaagct
     6361 ttgtgataat atcgttaatt gtaaattgaa gttcacgcgc tttttctaaa tctttatcaa
     6421 caattaactg atttaaagtc aagtataatt ctggcattac gccataggta ccaccaatac
     6481 ctgcagcggc tcccatgaga cgaccaccca aaaattgttc atctggacca ttaaaaacaa
     6541 tatgattttc tcctccaata gccacaaaat tctgaatatc ttgaactggc atggaagaat
     6601 ttttaacacc aattacttgc ggattttgta acatttttcg atataaatcc gatgtcaaag
     6661 caacccctgc caattgagga atattgtaaa taataaaatc agtttgtggt gctgcttgac
     6721 taatagtatt ccaataatct gctatggcat actctggtaa tcgaaaataa attggtggaa
     6781 ttgctgcaat agcatcaaca cctatagctt cagcatgcat agccaactct acgctatcct
     6841 tagtattatt acatgccaca tgcgctatta ctgttagttt acccttagca acggacataa
     6901 cattttccag tacaagttta cgatctgcta cactttggta aatacattct cctgatgaac
     6961 cattaacata aagtccttga acacctttat caataaaata attggtcagg gcttttactc
     7021 gttcgggaca aatatcaccc ttatcatcat agcaagcata aaatgcagga ataattcctt
     7081 gatatttttg taaatctttc attgataatc tccttgttgt ttgctgaaat aggcaaattg
     7141 tttttgtaaa ataataattt taaagtaagc aaaggcggta taagcaaaaa taaatatagc
     7201 tgacactgta agcataaata agagactaga ctgcaccata ataaaccaag tcatgattaa
     7261 taaaaacatc aatacaccag tccatttcaa gtttaaacat gccattataa agctcttttt
     7321 cacggtatct aaaagagaca tttcgtagcg ggcagccata ggaaataggt atatgctcgt
     7381 catcacagtc aataacaaaa aggcgtaaca agttatcttc aagaaagaca caatgaaacc
     7441 tatttgataa tgaagaattg ttaaatcaaa tatgatcaca accaaaatac ttagttcaac
     7501 caaacctaaa agcatacctt ggaaccactc tttttttagg tgttttatat acaatattaa
     7561 aagatttgtc tgatcacctt ctaatttttg ccacaacgtt cgatacaaac taattttagc
     7621 agcaccaata gttaccaatg gtaggcaagt tatcataaaa agaaggttta gtaatgctag
     7681 atcaaaaaca acattgcaac cttgcataaa ggggttgtta acatcaaaaa tcgctgctat
     7741 taattgattt gcttttttca caatatactc cttgtatttc ttacgccatt aacactttca
     7801 ataccccttt tttgacataa ttatctagac ttcccatacc atttggctga tgagcttctt
     7861 ctgggaaaaa gaaggctata taattatctc tcaaatcaaa agaagtctct tctgagcact
     7921 tgataaatcc tatatcgcct aagtattcct ctacctctac cttatcacct tttcccaatt
     7981 tgataacttc atgtccttca atgacataat gtatatcaag gtatttttta tggtattcaa
     8041 aaatatgatc aaatgactca ctaagttgat ttgattggac catgagtatc acttctgggc
     8101 taatatgata ggtgcctata tctaaattcc ttaaatcatg acttagtaga taatcaatcg
     8161 ctaaatctaa attaggatta atatctttat aatgcgttaa attcaataaa tgatcgtaaa
     8221 tcataataaa tcctacctat cctttaacag ctcccattgt aatcccctga gtaaaggatt
     8281 tttggaaaac taagaatact gtcacaatag gaacagcagc taaggctgca cctgccataa
     8341 ttaagccata gtttgttgcc atttcagcct gcatagtagc taccccaaga gaaatggtca
     8401 gattatttct agaagttagc ataactaatt gcataaaata gtcattccaa gtattgataa
     8461 atgtgaaaat agctagggca gcaaatccag gtttgacaat tggaaatgct acattaataa
     8521 atgttcgaat ctctccacaa ccatcaattt tagctgattc caataattct gttggaatat
     8581 tttcagaaaa ctgtttcatc aagaagaccc caaaaggcca accaacaaga ggtaaaataa
     8641 ccgcccataa ggtatcatgt atccccataa aattgataat tctaactaat ggcactaaaa
     8701 caacctgttt aggtaaagcc atagcagcaa taaaaaggga gaacaatatt ttttgaccgt
     8761 aaaatcgttt cttagctaaa acatagcctg ccattgacga tgtacagcaa actaggaaca
     8821 ttgtcataat tgagataaag acactattcc aaagccatct caaagctggg ttttgtacag
     8881 ttaaagcttt aaaattttct aaggtcggtg ctttgggcca ccattgtggg gggatgataa
     8941 tagtatcagg ttgagattta aaagctcctg tcataatcca ataaaatgga aagataaaaa
     9001 gcactgtcaa aagacaaagg ataagagccg ttaaaaagtt ataagcactg aatgttttct
     9061 ttttcatgtt ttcctcctaa tattccacat catttcctaa gatcttaaat tgagcaaaac
     9121 ttattaaagc aatcattact gctaggaata ctcccatagt attagcatag ccatattctg
     9181 atagtttaaa ggctttttca taaagataat acatcaaagt gcttgttgaa taatttggtc
     9241 caccagatgt caggagctgg attaaggcaa aacattgaaa tgaattaatt gttgttataa
     9301 ctgcaatata gagtgtagtt ggtaagagac tcggccattt aatttgccaa aaaacttgca
     9361 tttcattggc tccatcaacg cgcgcagctt cgcaaagaga attatcaata tttcccatcg
     9421 ccgcaatata tagaataata ggttgaccaa ctgaggtcgt tagcaaaata ataataatag
     9481 caagtaatgc ccagtgctta tcaccaagcc agctaatatt ctgttcaatg acatgacctg
     9541 atttcaagat gtaatttaaa attccggaca tagggtcata aatccatttc caaacaactg
     9601 tgactgcaac actaccagtt actactggca agaagaagac acaacggtag aaagagcgtg
     9661 aaaaaacatt tttttcataa gtattggcag caacaaaaag tgagaagaaa accactactg
     9721 gcacagagcc aatcacaatg attactgtat ttatcaagga tttcatgaaa atagaatcat
     9781 gaaacatacg attgtaatta gctaacccaa taaaagtgaa ctgtgtcata gaataattaa
     9841 aaaaactagt gacaaagccc ataaccatcg gtgcaaaaac aaaaattaag aaaaataaga
     9901 gaataggcgc tagaaaagca taagctatca tcgtttccct catctttaac ttattagtac
     9961 gcatacggta ctctccttta catcttaact tgactaaaaa ctcctcttct atcccaatat
    10021 gtgattgacc atagaaaagg agtctcctta attatttagc tgctttttta atggtatcat
    10081 ttgctttttg agtaaagtct ttcaaagcat ctgccggttt ttcatcacca ttggatacag
    10141 attgaaccat tgggaaccat aaggttctca tttcagaaaa tccatcgata gtgttgtaat
    10201 atggtgaata atattgagtc cattttgaaa tcttcatcat acgtttatca cctttataaa
    10261 gatccccaaa tgatgttcta actgggaaag cacctgtacg tataacatct tttggtcccc
    10321 attttttatc gtcagcaata aaagtgataa atttcttaga ggcttttact ttgttttcat
    10381 ctttattatt aaagaccgca aaaccattaa caaggtattc taaatctggt tttccatctt
    10441 ctgatgggaa tggcacttca aggtaatcca ctttacttga ctctaataat tttgcttgag
    10501 tttttggttg agctggagcc cataggatag tgaaagcagt ttgtccatta gcaaagtttt
    10561 gaatgtcagc tgagccatca tactgagacc cattcatcaa gtagcctttc ttaatccatt
    10621 caactatttt tttcattgat tttacagaat ttttagtgtc agtggtatat tttgttactt
    10681 ctttatctgt tattggagca ctataaagat tagcaaaaaa tgcacgtggt ccttgatctc
    10741 ctccttgccc gtttgcaaag aatgaacctg gtgtatagcc tttatttttt agtgctttta
    10801 gtactttttc aaaatcacta gtagtccaac cttcttttac aagtttcaaa actcctgcat
    10861 ctttaagcat ttttttattg aacgccatat aaaatggggc agaacttatt ggatacatgt
    10921 aggctttatc gccagactta gaagcttgaa tgatgttctt attattgaca tccttaataa
    10981 attggtctgt aaataaatca ttcaaatctg ctaatttacc atttttacca tattgaataa
    11041 ttcgccctgg tgcatcaaaa agcacatcag gtgctgtccc tgcttcaatt gctgtagtga
    11101 ttttttcagg tccagatgtg aaatcaattg tctctagttt tacttttata ttaggatttt
    11161 tcttttcaaa agccttgatg acttttttct cataagtacc tactccatcc ttagcctttt
    11221 cttgagtaaa ggttggaaaa gcccaccacg taatttcgac tttatccttc tcggaagcca
    11281 taacagattg actactgtct acacaagcaa acattgataa tgttgcagct ccgaggcaaa
    11341 aaccaatcac actttttttg atactcataa ctattcccct tttttatttt gattttgatt
    11401 tttgagtaac acttttaact aagtcctgag atgaaacgct ccgctatttc ttttggtcta
    11461 gtgatagcac caccaactac aattcctgca acacctatat gattaatttc attagcctgc
    11521 ttaggagtat gaattttacc ttccgcaatc acatctatac cggcttgaca aagcttatta
    11581 aggagttcta tatccggtcc ttcttcttgg cggctgtaat ctgtgtatcc cgatagagtt
    11641 gtacccacaa aatcaactcc tgcttcaaaa gcatttttgc cttcttcaaa agtacttata
    11701 tcagccatta gcaactgttc aggatatttc ctttttatct tttgaataaa ctcagctaca
    11761 ctcaaaccat catgacgctc tctaagtgta caatctaagg ctattactgc aatatctaaa
    11821 ctagctaatt gatccacctc tgtcatcgta gcagtgataa atggttcttg tggaggatat
    11881 tcacgtttaa taatgccgat gataggtaaa ttagtaactt cttgaatttc cttaatgtcg
    11941 cggacactat tggctcttat accaaccgct cctgcttctt gagctgccaa agctaaaaga
    12001 ggcataacac ctccgctttc agtataaaga ggctccccag gcaaagcttg acatgacaca
    12061 ataatgccat tttttatttg ctttttaaaa gcttctttac ttagatgtgg catattctca
    12121 cttccttatc ttttttaatt ttagatatct acaaacctta ttatatctat ttttaaagcg
    12181 ttttcaataa tctttacaga cttagatttt agttttaacg ttttgattta gtcacggtta
    12241 acgtgaaagt agtttcagac ttatagctta atctcctcaa tttaataaaa attagataag
    12301 aaccttgaaa gtctat
//
LOCUS       DATRSD010000063        11473 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRSD010000063 DATRSD010000000
VERSION     DATRSD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.064"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(219..947)
                     /gene="lrgB"
                     /locus_tag="VBD85_001479"
     CDS             complement(219..947)
                     /gene="lrgB"
                     /locus_tag="VBD85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO7134028.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLILIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(949..1404)
                     /locus_tag="VBD85_001480"
     CDS             complement(949..1404)
                     /locus_tag="VBD85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7134029.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(1574..2314)
                     /locus_tag="VBD85_001481"
     CDS             complement(1574..2314)
                     /locus_tag="VBD85_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO7134030.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASVELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(2295..4040)
                     /locus_tag="VBD85_001482"
     CDS             complement(2295..4040)
                     /locus_tag="VBD85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein LytS"
                     /protein_id="HEO7134031.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVGALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(4067..4711)
                     /locus_tag="VBD85_001483"
     CDS             complement(4067..4711)
                     /locus_tag="VBD85_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7134032.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVARDGVRPIKGAQRLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(4834..5229)
                     /locus_tag="VBD85_001484"
     CDS             complement(4834..5229)
                     /locus_tag="VBD85_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7134033.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            5310..6026
                     /locus_tag="VBD85_001485"
     CDS             5310..6026
                     /locus_tag="VBD85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7134034.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TEQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRDLPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNITGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETGWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(6046..6672)
                     /locus_tag="VBD85_001486"
     CDS             complement(6046..6672)
                     /locus_tag="VBD85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO7134035.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIVIEGAGQITLSDQDIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQAEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(6705..7028)
                     /locus_tag="VBD85_001487"
     CDS             complement(6705..7028)
                     /locus_tag="VBD85_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO7134036.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINYK"
     gene            complement(7025..7309)
                     /locus_tag="VBD85_001488"
     CDS             complement(7025..7309)
                     /locus_tag="VBD85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO7134037.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREF
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            7470..7709
                     /locus_tag="VBD85_001489"
     CDS             7470..7709
                     /locus_tag="VBD85_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134038.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     TSFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            7894..8961
                     /gene="pepA"
                     /locus_tag="VBD85_001490"
     CDS             7894..8961
                     /gene="pepA"
                     /locus_tag="VBD85_001490"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO7134039.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAANGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            9031..9801
                     /locus_tag="VBD85_001491"
     CDS             9031..9801
                     /locus_tag="VBD85_001491"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO7134040.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIIISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAETNFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(9822..10487)
                     /locus_tag="VBD85_001492"
     CDS             complement(9822..10487)
                     /locus_tag="VBD85_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7134041.1"
                     /translation="MSFIIRKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKALLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(10556..11011)
                     /locus_tag="VBD85_001493"
     CDS             complement(10556..11011)
                     /locus_tag="VBD85_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134042.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRVLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFILYGYLLIYFILQKLL"
     gene            complement(11052..11189)
                     /locus_tag="VBD85_001494"
     CDS             complement(11052..11189)
                     /locus_tag="VBD85_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134043.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFIITISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(11248..11454)
                     /locus_tag="VBD85_001495"
     CDS             complement(11248..11454)
                     /locus_tag="VBD85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134044.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
BASE COUNT         3836 a         2261 c         1765 g         3611 t
ORIGIN      
        1 taaataatat ttttgacaac ttttttcatt ataacaagaa aaaagtagga gaacaattac
       61 ttaactggtt aattagaaaa tttgttattt tatcattaat aacttaaagt taggtaaaac
      121 acaattctag acactagctc ataaaccatg ttaatccaat gtattttaat agacatcata
      181 aaaaggctga ataaattcag tcttaaccac gctattagtt atagcaaacc aatcaaatga
      241 ctaaagatag gaataaccaa atcaactacc aaaccgataa ccaccacagc tatagctgcc
      301 atcgcacctt gcagttctcc caattccaat gcaaaggctg ctccgactgt atgaccagag
      361 gtccctagac ctaatcctgc accaatctta ctatcattca aatggaaaaa tgacactaat
      421 tttttaccta atgcataaat aataactgca tttaagatac acgccattgc tgtcacagct
      481 gtgttccctc caattgctgc tgttattgga agagcaattg ctgttgttgc tgcttgtggc
      541 aacattgaag caattccgac ttgactaagc ccaaccattt tagaaatagc gacgataagt
      601 ataagggaaa caatcatacc gattactaaa ctgctgagaa tttctaccca atatttttta
      661 acaacgtcat ttttcttata gagaggaact gcaaaagcaa ttgtcgctgg atttaaaaac
      721 caaaatatta aatcaccacc tggtttataa gcttgtgtat aaaatgtctt aacatcggtt
      781 cctaaacctt ttgacataag ccaaagaatg acaataccac taaccattgc aaagaaaaga
      841 ggctgcaaaa ggaagaaacc tttactcttc ttaaataaat gttgtcctat cgtatagagt
      901 attaaagaaa agcaaatacc aaagatgggt gtttttaaga gttccatatt atttttcctc
      961 tactttctta ctatagtggt taggtaatat ctttttaaat aaatctgtga cactgagttc
     1021 tctgatagca agtattaatc gtgcaacata tgctaccact accaacataa caacagttga
     1081 gattgtaata acagcaacta gttgcaagcc ttcagctttt aggatatcaa gattcgctgc
     1141 tactgatatt cctgagggga caaacataaa accaatcatg cttatcatca aagccccaaa
     1201 agagtcaacc cactctacct tgataatttt agcagttagc aatacataca ttagcaccaa
     1261 accaataacc gtcgttggga ttggtagtga tttcggtaat aacattgaaa tcatttgtga
     1321 aactaataga atagctgcat atattgacat ttgaataaac attggagctt cttttgtttt
     1381 ggtttcttta ctggtgtgtt tcatagtcac taccccttcc ttttattaca atgttttaac
     1441 ttgttttatt gatagctata ttgtacttca aaacaaataa aacgttttca tttttagtct
     1501 cagttaccaa aaaaagaagc cgaattgcaa attcaagctt ctaaacaaca aagggaagtt
     1561 cgcaaaagca cttttacatg gttaaatgct catttaggtc ttttaaatac gatcgaccta
     1621 ctggaaattt ctctccatta gacataatta gtaacaaagt atgattaaac caaggttgaa
     1681 tttcctcaat catctctaga ttaatcactg tgtttcgatg aatctggaga aaatatggtg
     1741 ctacagcgcg tgacttaaac caattgagtg tcttccgaat agtgtaagtt ttctgaactg
     1801 tactaacagt taattcgcca tcttcaacac tagctgcaac aatatcttgc attttaacaa
     1861 caacactgcg atctgataat gtcaaggtta acaattcaac actggctttt ttaggtattg
     1921 cttgctctac atctgaagca gtggtgagtt ggctcaattt cttcactttt gatagagcca
     1981 tatcaacgcg ctgttgttca aacggtttca tgatataatc aacagcgtta ctttcaaaag
     2041 ctttcacagc gtagttatca taagctgtcg caaaaacaac aagtggggga tgtgcaagtt
     2101 ggctcaactg attagccaaa gtaaagccat tttcttcact aagcgaaata tctaaaaaaa
     2161 ttaaatcaat ttgctgtcta aataagattt tttcggcttc actgatatcc tcagcctgaa
     2221 aaatctcagg gttgtcaacc tcctggctat gttcaactaa aaacgataat tcttgtctgg
     2281 caaacatttc atcatcaaga attaaaattt tcatgctcat cctcccttat tctattaggt
     2341 attcgatacc aaacttttgt accattcttg tcgctcgaaa aatgaaggca acttacacta
     2401 ccatataata aattcagcct gttatttaga ttaactagag cagtacctgt acccttactc
     2461 tctgcaactg tttcttgacc taatttatca atgatagtat ctgagattcc ttgtccattg
     2521 tcactaacag aaacacaata ataatgacca tctggcttta tttgaaccaa tatatggttg
     2581 tccgtcttac gttctttgaa agcatgtcga actgcattct ctaccagtac ctgtaaacca
     2641 aaaggtggta acttcatttt ttctggtgca ctaatatcat aagataactg atatttatca
     2701 gggaaacgta atttttcaac attcatataa gcatccacat gtgatttttc ttgctcaagc
     2761 gttacctcac gatcctgacc accctgcaaa cttgttctaa aaaaagtact taactgcatc
     2821 agtgcataac gtgctttatc agaatcaata cggattaatg cactaattgt gttaatggca
     2881 ttaaagaaga aatgagggtt gatttgtgct tgtaaagcct ttatctctgc catactggct
     2941 aacttatttt gttcctctgt tatccccatt gccagttgtc ctgaaaatat ttgcgctaaa
     3001 ccaaggacta ggttttcctc cacctcagac attgtcttat ctcctgcaaa gtacattttt
     3061 aaggcaccca cagttttatc atttattttt agaggaacta caatagcaga atttaactga
     3121 cagttgtgat ctggacaaga aatcgccgct ttatcttgcg caattcttgg ttcgccatca
     3181 aaaataacac ttttagataa gtctgttttg accggttgtc ctgcaatatg gtgatcatgg
     3241 ccaacaccaa tatgagctaa tacgtttgac cgatctgtta atcccacagc atcaaagtta
     3301 gtatgcctct ttataatttc gcaaacgctc ctagcagatt gcggtgtcaa accttgtcta
     3361 aggtagggca gagtctgtcg agtcaattca agaacatctc tcgtttgaac tgcgcgtaac
     3421 tgactttcat ttgacaaata agttttcaaa atcgcaagga aaagtgtgga acctaaacta
     3481 tttaaaatca tcattggaat gacaatcatt ttgacaagtt cccatcctgt aaaaatgcca
     3541 acaaatagca tctggatact ttcggcaata atactaatta aaataacttg gcttgttgaa
     3601 gggtagagat ggttttcctt aagcttatca ccaatctttc cgctaacaat gccgactaga
     3661 actgaactga caatatagaa agaacctgaa aagcttcctt gaaaaaagcg atgaactcct
     3721 ccaataaaac caacaattga tccaaccaga ggtccaccaa ccaaacttgc cgttgtaata
     3781 actaaagtcc ttgtattagc aagtgagtca gaatgagaaa tcgttgttag aaaagggcgc
     3841 tcgaccaaac ttcgatcccc ttttatttca attcctgtta tattagatat aataacaaac
     3901 aagccgaaaa tgatgacaag gactaccgtt tcacgtttag accgctcttc aattaactgt
     3961 ctgaaataac tattatttac caataaaaag gctaaaatca taataattcc tagcctttgg
     4021 aataacaaca ccatcaacat atgcttatcc tcctcaagat accattttac tccggaagat
     4081 aaatatagat atcttgataa tctgaaataa ccttgtctgc cattgataaa tcttgcggtg
     4141 gataatcagg attagcaaag ccaaagcaat acattcctgc tgccttagca gctaaactcc
     4201 catttcttgt atcttctata actatacaaa ctttagggtc tacatctaaa agctctgcag
     4261 ctctaagaaa aacatctggt gctggtttag aagacgatac atcttctcct gtaaccatgt
     4321 attcaaagca ttctgtcaca cccaactctt ttagattacg cttaatatct accattggag
     4381 atgaggacgc aactgctaag cgatagccat gttggtgtaa ccaatgaatc agtcgctgag
     4441 ctcctttgat gggcctaaca ccatcacgcg ccacaatcgc ttgtcgacgt ctgttcatct
     4501 cagcaatata ctcttttact gttttcggta agccaaactc ctctttcata gcttgccaca
     4561 tgaattcaaa agtagtcccc atgtactggt attgatacga gacgtccgta tcaataccct
     4621 cctcacgtaa catttctgtc ttattatcta aaaacgtgta ctcggaatca acaataacgc
     4681 cgtccatatc aaaaataatg actttttcca tcttaaacct gctttctatc ccctttattg
     4741 taatcgtttg cctttctatt ttcaataaaa aaggttgctt tctgtgaaaa gcaaccagta
     4801 tatttagaaa tgatattttg agagtgattt tatttaaaag gggagctcct cttcttccaa
     4861 tactaaatct gacaaatcac cagaaacgtt attttcacgc atagcacgtt gggcacggct
     4921 ttctagcaac tgaaatgatg atgctaatac ttcagtgata tagtgcgttt gaccatcctt
     4981 ttcgtacttg cgcgtacgca attcaccatc tattgaaatt aaagagccct ttgtcccata
     5041 gctcgcaagg gtttccgcta gacgacccca cataaccaca ttaataaaat ctgcttcacg
     5101 ctcaccatta cttcctttaa agcgtctatt aacagcaaca gttgcacgcg tcactgactt
     5161 gtcagttggt gtttttacca tctcaggctt tgctgttaga cgcccaatca taataacttt
     5221 attatacatt gattacctcc tcactattat tattcgaaaa taaggaagaa aaatgctaaa
     5281 atatatgaaa ttgttctggg agaattaaga tgacaaatgt aactagttat aaagaaatgc
     5341 tggctaaacc ttggggaaaa atacaatatg aaataacttt tgctcaatta agccatatca
     5401 aaaatcagaa cgttttagat tttggagctg gtttttgtct caccgaacaa catctagcaa
     5461 aagaaaacaa tgtaactgct attgaaccca atcctaaact gctttacgat aatcaaagtg
     5521 acaatattta taaaatccta ggttcctacg aagcattaag agatttgcct gatcaaagct
     5581 ttgatactat tatttgtcat aatgttcttg aatatataga caaacataat catcctgctt
     5641 attttgacga attttctcga cttctaaaac ccaatggaga actatctctc atcaaacata
     5701 atataaccgg taaaatatta caatcagtta tttttagcaa tgatacctcc actgcgatgg
     5761 aactcttaac aggagaagca aactttaaaa gtgccagttt tgaccaggga aatatttata
     5821 ccttagaaga actaaaacaa aatacaaatt tattagttga acgttatcag ggtattcgta
     5881 ccttctattc attacaacca aaccatttta aaactgaaac tggttggcta aacaaaatgc
     5941 ttgctatcga gctaagtgtt gctgacaaag ctccttataa agatattgct tttttgcaac
     6001 acatcacact taaaaagtca ttgtaacttc ttaagttgat ttttactaat ttttccaatg
     6061 tttattggcg tcgaaagact ctccaacaat tacctgatca gataattcaa tgatacctct
     6121 gacttgagga gcatttggaa gagctaactc gcgagcacta cataacattc caaagctatc
     6181 ttctccacga agttttcctg ggaaaataag gctaccattt ggcatcatag ctcctggaag
     6241 agctgcaact gttttaagac ctactgaagc attaggtgcc ccacaaacga tttggaccgt
     6301 cttcccatcg ttgatttctg cttgacagat atgtaaatgg tcactatctg gatgatcaat
     6361 catttcttta atttgcgcca caacaaatgt aggttcaata ttattaacaa gagagtcttc
     6421 gaatccttct ttcagtagtt ctgcattaag gattttaata tcttgatcag ataaagttat
     6481 ttgacctgct ccctcaataa caattaaact tgacacttca aagatattcc aagcaactgt
     6541 ttctttacta tcttgcagat agacgcgtgc tacttgtccg cgacgatcaa catctagctt
     6601 agctccttga ctatccttaa caataaccat taaggtatca ccaacatgct ctctattata
     6661 agtaaaaatc atttattctc ctttaaattt tgctattttc tgtcttattt atagttaatt
     6721 tcagcgagaa atttagtaat ttcagccttt gttttgcgat tcttattaac caaacgaccc
     6781 agttcctgac cattttccac aacaacgaaa ctcggaatcc caaaaatatt ccactgctga
     6841 gctaattcta tatagtcatc acgattaaca cgtacaaaga caaaatctga gaaatctttt
     6901 tcaattgatg gcattactgg atatataaat tgacaatctg gacaccaatc tgctgtaaaa
     6961 aagaaaacaa ctttcttagt gctatcaata taggctgcta tttcttcata agattctggt
     7021 aaaatcattt gctcacctcc acataatcaa gaacaccttg acgataaatg aattcgaaaa
     7081 ttctgccatc ttccaagaca agaccaccac tggtcatttt aatatcagat tcaaattcat
     7141 taaaataaaa tacctgaata tcacccatct gactaaaaaa ttctcttaag gtctgagtga
     7201 tttgctgacg tttataatct gttacttttt tagttagggt ataaccagca gctgccagta
     7261 aacccacacc agcaaggcca gtcccaaata agattttttt atttttcata actgttatta
     7321 taccataccc cccggaatta gacacaggaa ttgatggaag aaatccacat aacagttatg
     7381 aaaaaaaaca acttattcct tgtggtataa taggatgaca ttgtcttaag ataccattat
     7441 ggttaatcat tcaaacagaa agaaaaaaca tgaaagaaaa attattagta cctgctttaa
     7501 attgtttgct aggtctactc ttcctaggtg atcactggtt tttatttcgt cttcaaaatg
     7561 gagaactttt tgctattgtg acgctaatac tgctttgcac aacttcattt ttaatcaata
     7621 agaatcaccc caaaaatttg actttaattg ctatggttat cactttttta gccgtattat
     7681 cttttctatc taccttttta ttagcttaag ccgataaaca ttatctccgt tgtattcaac
     7741 ccagttttca ctataatagg ctttgtcagt cataacagtc ttccggtagt acttgatatt
     7801 ctttttgaaa gtctttatat tgactgaaac ctgttctgtt ctttattttt aattaaaagg
     7861 tgctaaaata gtatttgtag aattgaggta attatgtcag atttatttaa caaaattaaa
     7921 accgtaactg agcttgatgg gattgctggc tatgaacaca atatccgcaa cttccttcgt
     7981 caagaaataa ctcctttagt tgatcaagtt gagacagacg gacttggtgg aatttttgga
     8041 gttaaaaata ctcatgagac taatgctcct aaagtcatgg ttgctgccca tatggatgaa
     8101 gtcggcttta tggttagtca tattcagcca gatggaacat ttcgtgtact tgaggttgga
     8161 ggatggaatc ccctagtagt cagctcacaa cgctttaccc tctacacacg ttctggtgat
     8221 gctattcctg ttatatcagg ctcagttcct cctcactttc ttcgtggaca aagcggtgga
     8281 acaacattac ccaaaattag tgacattgtt tttgatggag gattcacaga taaaaatgaa
     8341 gctgaaagct ttggcattgc tcctggcgat atcattgttc ctaaatctga aaccatttta
     8401 actgcaaatc aaaaacatat tatgtcaaaa gcttgggata atcgctatgg tgtgcttatg
     8461 gtgaccgaat tgctaaaaag cttaaaagat caaagtctta gcaacacact tattgctggg
     8521 gcaaatgttc aagaagaagt cggacttcgt ggcgcacatg tttcaacaac taaattcaac
     8581 ccagatatct tcttagctgt cgattgttcc ccagctggag atatttatgg ggaacaaggc
     8641 aaaataggag agggaacctt aatccgtttt tatgatcccg gacatatcat gcttaaagat
     8701 atgagagatt tcttacttac aacagctgaa gaagcaggta taaaatacca atattatgct
     8761 gcaaatggtg gtaccgatgc tggggctgct cacctaaaaa atagtggtat tccttctaca
     8821 actatcggtg tctgtgcacg ctacattcat tctcatcaaa cactctacgc tatggatgat
     8881 tttctacaag cacaagctta ccttcaggcc atcgttaaca aattagaccg ctcgacggtg
     8941 gatattatta aaggttatta agatagaaag gatgggagtt tcataaggta gacacatttt
     9001 agtatctcct ttcatcccca tatcaacatt atgaaaattg gaattatcgg cgtcggaaaa
     9061 atggccagtg caattattca aggcctcaaa caaacccaac atgacattat catatccgga
     9121 tcttgcttag agcgttcaaa agaaattgca gaaagacttg atgttactta cgccgaatct
     9181 catcagtcat taatcaatca agctgatatt atcatgttag gtattaaacc tcaactattt
     9241 gaaaaggttc ttcttccact tgatatcaca aaaccaatca tctcaatggc agcaggaatc
     9301 tccttagcgc gtttaagcca attaactcgt tctgatttac cattgattcg tattatgcca
     9361 aatattaacg cacaaatact tcaaagttgc acagctattt gttacaacaa tcatgtatca
     9421 gatgagttac gacaattagc caaagaaatt acggatagtt ttggaagtag ctttgatata
     9481 gcagaaacca attttgatac cttcactgct ctagcaggtt ctagccctgc ctatatctat
     9541 ctatttatag aagccttagc taaagcaggt gtaaaatatg gatttccaaa agaacaagca
     9601 ctctctattg taggacagac tgtcttagct tctagtcaaa acctattaca aggacaaaat
     9661 agcacctctg acttaatcga caacatttgt agcccaggcg gaactactat tgctggcctt
     9721 ttagacttag aaaaaaatgg tctgacgcat agtgttattt ctgccattga tgctactatt
     9781 gagaaagcaa aaaaactttg agcaaattgc tcaaagtttt tttagttatt catcattgta
     9841 ctgattaaca cgctataagc aagagcattg ttaataaaat gaatcattat actatattct
     9901 aagtgctcag ttttataata aacaaaagat aaaatagcac ccatcccagc ataaataata
     9961 aaactaccta tatcagaagg ggaatgaata agagcaaaag ctaaagtacc gatgacaaaa
    10021 ccccattttt gatgtttcgc aaaaagttct ttagggataa tatagcgaaa tataatttct
    10081 tccataatag gggcaccaat tatggcaaaa attaacatca aagctagtgg aagttgcttt
    10141 gttaattcca taattactgt ttgatttgct gtatctttaa ccccttgcat agtcataatg
    10201 aggtaaccaa taatttgtgg aaccctcaat gataaccaac ctaagagaat agtaactaat
    10261 gctttccttg tcaataatgc tttccatctg attatttgtt taaccttggc ataataaagg
    10321 aaaagagcaa taataagtaa ttctaagcca atcaacaata tactagtaaa aggcgtttga
    10381 tagtgattct ttaggagcca cacagccaat ataggaacaa gttgattaat aactaaaaat
    10441 gctattagca aagctaaaat tttgcctttt ctaataataa atgacacaaa aacctccaaa
    10501 ataaattatt aacttttttc tatcataaca tattttcaaa tcttatagac ttatctcaca
    10561 ataacttctg taaaataaag taaattaata aataaccata taaaataaaa gagagtatca
    10621 atagcaagaa ctttgcatag gaaaaagtta taccaaacca gacattaatc agacacaata
    10681 ccactaaaac aaggacagat aaaaggaaaa gaagtcgaca agacatatta gcaataaatt
    10741 gattacgctc atcaaaaact ttggtatagt aatgttgaaa tcgcttttca ctaaatgtta
    10801 tcaccaaaaa ataaaaactt cctgcaaata aggcaatacc tgaaccaaag gaaaaacctt
    10861 tagtaaaatt agattcaaag gtatgtagaa atgatgatac tatcataatc aaactacata
    10921 cactaaaaac aaaagacaaa actctcatat atactttgta atccttcgga gatactttac
    10981 tattgggtga caataatcct tttattaaca tagaatacta ctttctaaaa cttcaattta
    11041 atcatcagct attatatcgt tttcttcaat gaagcatagc gataaatagc aaaaatagac
    11101 atcgcaatgc taatactaat agtaatgata aaaacatatt gctggtaaac gattggtgga
    11161 caaaaatcaa tagtccacat ccttgacata ttacagatta acttttaacc ttttgtcttt
    11221 tgtggactgt atttttccat taaaattcta tcctccttca ctttctgtat aaataaaaac
    11281 ttcctcaatt tgtttatcaa aataacgcgc tatcttaaat gctagctcca aagatgccgt
    11341 gtatttctct ttttctagag aaataatagt ttgcctagta actccaagtg caactgcaag
    11401 ttctgcttga cttaatttcc tagattttcg taacttttgt agagaatttt tcatatctta
    11461 cacctttttt gta
//
LOCUS       DATRSD010000064        11267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRSD010000064 DATRSD010000000
VERSION     DATRSD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.065"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            958..2082
                     /gene="mutY"
                     /locus_tag="VBD85_001496"
     CDS             958..2082
                     /gene="mutY"
                     /locus_tag="VBD85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO7134045.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            2259..2852
                     /locus_tag="VBD85_001497"
     CDS             2259..2852
                     /locus_tag="VBD85_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134046.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2898..3212)
                     /gene="trxA"
                     /locus_tag="VBD85_001498"
     CDS             complement(2898..3212)
                     /gene="trxA"
                     /locus_tag="VBD85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO7134047.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3293..3793)
                     /locus_tag="VBD85_001499"
     CDS             complement(3293..3793)
                     /locus_tag="VBD85_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7134048.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3797..6136)
                     /locus_tag="VBD85_001500"
     CDS             complement(3797..6136)
                     /locus_tag="VBD85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO7134049.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVYDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLSPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(6221..6763)
                     /locus_tag="VBD85_001501"
     CDS             complement(6221..6763)
                     /locus_tag="VBD85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO7134050.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6766..7077)
                     /locus_tag="VBD85_001502"
     CDS             complement(6766..7077)
                     /locus_tag="VBD85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134051.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            7188..8081
                     /locus_tag="VBD85_001503"
     CDS             7188..8081
                     /locus_tag="VBD85_001503"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO7134052.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            8097..8690
                     /gene="lepB"
                     /locus_tag="VBD85_001504"
     CDS             8097..8690
                     /gene="lepB"
                     /locus_tag="VBD85_001504"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7134053.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGPFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            8819..11239
                     /locus_tag="VBD85_001505"
     CDS             8819..11239
                     /locus_tag="VBD85_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO7134054.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
BASE COUNT         3805 a         2113 c         1794 g         3555 t
ORIGIN      
        1 aaagacattt tttatttttc tttattgaaa tatgccttat atcaaaccta tgtagccatt
       61 ttcttttact gacttatgtt caatccccat tctataattc aattaaaaaa taaaaaggcg
      121 aggtttacac ctcaccttca acatattatt taaagaattc tttattggtt taagttttcg
      181 ttggagaaaa attcagtgtc tccgatttta tatttttaaa tattaaaata ctatttaggg
      241 agtccctctt tatgttttat tacctttggt aacaccaaaa catacacgaa cttttattca
      301 tatgtgttac cattttacat ctttcatatt gagatatttg attagcaata cctccttgac
      361 taacaaaaat tactaacaca tctaggggta taccctagtt tatatttgtt catgattacc
      421 tcaagcaagc atgcgctaat ggtaatgaac tattaccaca ttgtctgtct tccttaaatt
      481 atataaggac ttatgtgtca ccgttactgt agcgacctac atctaaacct aacataagtg
      541 cccctccaac tatttctagg aaatgaagaa aacttcaaat ccttgtgact gtttgcaaca
      601 tcaatgcatt gataacaatt ttagtgtatc gccattctta tagcgaccca tattaaaaat
      661 taacataagc gctacctcca ttaactaaag ttagcttgcc taccaatctt tcttttggag
      721 actagtctcc attcttatac ctagagatat tgtaatagat catggataac ctccttgatt
      781 taaactttat ctttataaat taattatagt gtaaacgctt ccttaagtca agaaaaaaat
      841 attgatgcaa catttttctt catcattaag tacgatctag tcaacatacc tctttgtcaa
      901 atcctctttc tttgatacaa ttaatctatg atagatttaa aagactatgg aattgagatg
      961 tggcctgaag atagaatcgc ttcttttcgt cgcacacttt tagggtggta tgaccaagaa
     1021 aaacgtgatc tcccttggcg aagaactact aatccatact atatttgggt ttctgaaatc
     1081 atgttgcaac aaacacaagt taatacagta atcccttatt ataaacgttt tctagagtgg
     1141 tttcctcaaa taaaagattt ggcagacgct cctgaagaac aattactaaa ggcatgggag
     1201 ggactaggct attattctcg ggtgcgtaat atgcaaaagg ctgcacagca agtgatggtt
     1261 gattttggtg gcatatttcc tcacacttat gatgatattg cttcattaaa agggattggt
     1321 ccttatactg caggtgctat cgccagcatc tcattcaact taccagagcc tgctgttgat
     1381 ggcaatgtga tgcgtgtcat ggctcgtttg tttgaagtta attatgacat cggtgaccct
     1441 aaaaatcgta aaatttttca agctattatg gaaatactaa ttgaccctga cagacccggt
     1501 gattttaacc aagcacttat ggatctgggt acagacatcg aatccgctaa gacacctaga
     1561 cctgatgaaa gccctattcg tttctttaat gctgcttatt tgaatggtac ttatagtaaa
     1621 tatcccataa aaaatcctaa gaaaaagcct aaaccaatgc gaattcaggc ttttgtcatt
     1681 cgtaaccaaa atggtcaata tctactggag aaaaatacta aagggagact tctagggggt
     1741 ttttggtcat ttcctattat cgaaacgtca cctctttctc aacaattaga tttatttgat
     1801 gacaatcaat ccaaccccat aatctggcaa acccaaaatg aaactttcca aagagaatat
     1861 caattaaagc cccaatggac tgataatcac tttccaaata ttaagcacac ttttagtcac
     1921 cagaagtgga caatagaact catagagggg gtggtcaaag caacagattt acctaatgct
     1981 cctcacctaa agtgggtagc aattgaggac ttttctctgt atccctttgc aacaccacaa
     2041 aagaaaatgc tagagaccta cttaaaacag aaaaatgctt aatcacaagt gaactatgac
     2101 ggttcacttt ttgtttacaa ttcctttacg ccgctgtaaa gttttcctca gattatcttt
     2161 taatacccta taaatagtgg tctatcccta atgtcaaata catttgatag acccgttagc
     2221 ttatcatcct tatactaata ttatgaaagg agatatttat ggaaattggt cagcaaatta
     2281 ttcgctaccg taaacaacaa gcgctctcgc aagaagaatt agccgagaaa gtctatgtaa
     2341 gccgtcagag catatctaac tgggaaaatg acaaaactta cccagacatc catagcctct
     2401 tactcctaag tcaaattttt caagtgagct tagaccaact aattaaagga gacattgaga
     2461 aaatgaaata taccatcact caggttgata aaaagaattt tgaacgagat accaaagtaa
     2521 tggtgacatt gatgatacta ttaatgattt caagttatcc attagtctat ttcttagagt
     2581 ggctaggact tggcattttt gttttattat cgataattac tatgacttat gctaatcgtg
     2641 tggaacgttt taagaaaaaa tatgatgtgc aaacttataa ggaaatccta gctgtttcaa
     2701 gtggaaaatt attagacgaa atcgaaaaac gggaagaaag agcaaaactt ccttatcaga
     2761 aaccccttat tgtcactgta tttttcctca ttactgttgc cacctttttt gcaagtcgtt
     2821 ttattttcac atggttgttc catttaagtt agtagataat aaaataaagc tcagcgtgac
     2881 actgggcttt attttattta actcaattca gcaataatag ctttcagttg gtctttagta
     2941 tgaacacctg caacttgttt aaccacttct ccgtcttttt taaacatcaa tgttggaatt
     3001 gacataatac caaactgacg ggcagtttcc ggattctcat caacatccat tttcaaaatt
     3061 ttgagttcat cttcatcgat ttcttgagat aactgttcta agattggtgc ttgcatacga
     3121 catggaccac accaagttgc ccaaaaatca atgagtacta aaccttcttt agtttcttct
     3181 acaaatgtag catctgttac ttctaatgcc atcttttttc tccatttcat cttaatgata
     3241 agcctattgt acaagaatct agttgttttt gaaactattt gctacgaact cgctaaataa
     3301 ataaacaaag ccctaaaatc aagccaataa ggtaacctac aataacatcc ttaggatagt
     3361 gaatacctgc tatcacacgg caaattgcca atagaaccga taatattaaa caaactattc
     3421 caaagtaaac ataatatcgt aacaaacaca tactaatcat cgtagctgaa aaaacatgtc
     3481 tgctaggcat tgaccttccc tcagtatcct tataaattaa aggtttaata ttccattttt
     3541 catatggtcg tggaaaatca aggcgtttcc taatgtaaga caaagctata aagccaacag
     3601 cagggataat taatagcctt aaccataatt gaaatgtcat cccttgccaa agagtaaata
     3661 tcagaaaaga tggatagagt atgtacatca ttcctgtgac tatttttagt aaaaaattta
     3721 aaagaattat taactgtggg gattttctaa aaggttgaga tagtttggca taaaattgtt
     3781 catatgattc cataatttac cctaacgtta caattgtggc gccagatccc cctgcatttt
     3841 gtggggcata agcaaaatgc ttaacgtgct tattacgacg aagatatttt gtcactccct
     3901 cacggataac gcctgtacca ataccatgaa tgatatcaac ttgtcccata ttgttaagca
     3961 atgcttgatc aataaaatta tctaactctt gcatagcttc ttcgtatctt ttacctctaa
     4021 gatcaagtcg agctcttggt ccagaactat cagccttttt aaccacatta atctgtttac
     4081 ttttgacttt ctgttcttct tggactctaa caagggtaaa ttcatcttgt gttaatgtca
     4141 ttttgataat tcccacttgt gcttcccaac gtccatcttt taattgacta gttaaggtac
     4201 ctcgctgtcc atagctagtc actataatat catcaccaat tctaggagca cgagctgctt
     4261 taattttttt agccttattt aagactttat tttttgataa atcaacttga ggtgctaatt
     4321 tttttatttg tgccttagca tctataattt cgtgaggttt taattggctc ttatcattga
     4381 gtttttttaa gatagtatca ctctcattca aagccatatc tacaatttct tgagcttctt
     4441 gatagatttt ttctaactct ttatcgcgct catgtgaaaa ttcattatag agtttcttaa
     4501 ccgcacgatt gaatttgagg ttttcttgtt caacttcttt aatatgatcc agtcttctac
     4561 gtgtctcaag tgtctgtgcc tctaactgtt caataatacg gttaacatct gagtcagaat
     4621 ctgtcatctg cttagcttgt ttaacaataa atggagataa accaaggcga gaagcaattt
     4681 caaatgcatt tgatcgtcca ggaactcctt gcataaagcg atacgtaggg ctaagcgttt
     4741 cggcatcaaa ttccatgctc gcattctcta caaaatttgt ctcaatccca taagctttta
     4801 attctggata gtgcgtggtc gccatcgttt tgatattact taacctaagg tgttctaaaa
     4861 tagccatagc caaactagca ccttcttgag gatccgttcc tgctcccagt tcatcaaaga
     4921 ggactaaact attgtggtca gcctcgttta aaatactgac tatgtgcgtc atatgactag
     4981 aaaaagttga tagactttgt tcaatagatt gctcatcgcc aatatctgca aagatattgt
     5041 taaatactgc aattttacta cctttatccg ctaatactgg caaaccagac tgtcccatta
     5101 attgtgctaa accgagtgtt tttagcataa tcgtcttacc accagtattg ggaccagtga
     5161 tgacaattgc agttaaatct tgatcaaaat gtaagtcatt agccacaggg ttacttaaca
     5221 gaggatgacg aacattgatt aatgctaacg tgctgtcatt agaaatctca ggtatcgtcg
     5281 ccttattatc agacataaaa agatatttag cccttacaaa atcaagatgc ccaagaatcc
     5341 atgcattatt tctaatagtg gcgacatggg gtcttaacaa gtctgaaaat gcgtgtaaaa
     5401 tacgactttc ttcatgacgt tcgtcagctc taagctgcgt tatctcttcg tttagtgtaa
     5461 ctacagcacg aggctcaata taaacagtac ttcctgaaga agagatgtca taaaccacac
     5521 cagaaatacg attccgataa gtatttttta ctggtaggac acttcgtcca ctacgactag
     5581 cgattagatt ctctgataaa agctctgctt tttccttaag catatcctgt aaaatctgac
     5641 gaacccgtcg ttcactgttt gttaattgac gacggatacg ctctaattct ggactcgcaa
     5701 aatgttctaa aaaaccacca tcattgatag cttgaaaaga cccttgcaga ttagggaatt
     5761 gttccaaatt ttcaaacaaa cgatccaaag attggaaaga aacattatcc aaatcagaat
     5821 aaaagtgaat catatccgaa gaactctgta aaactttttt gatagccaaa agttcttgaa
     5881 tattaagatc agctgaaagc tctaagcgtt ttaaactctc agagattgaa cttaggctga
     5941 ctatgccaaa tgagtgatgt tcaacaaaaa tctgttccat gtcagaaatt tcattaaaac
     6001 ttttttctat tttaggagcc tccgtcatcg gctccagctc tgataattct tcttgtgatt
     6061 gttctgtctt gagataaggt aatatcaatt ccttaacttt gttaaattct aactgttcta
     6121 aaatcttgtt attcatactc ttcattatac ctaaatttat cacaaaatga caacgttaaa
     6181 aaggttggaa ctcttactcc aaccttacta aacattatat tcaaagacct accgcccaag
     6241 tccttaccaa aaactgtgag aagaatggta agtcgttaat taaaaatcta acaaacagac
     6301 tattccataa cttttcttga acaaatgaca tagggactgt tgccagaata gtcaaacaca
     6361 tactcataaa taagagacaa accagaacgg ctaaaaaacc actaataata ttattttgca
     6421 agctatcaaa atgttcaact ggtgctaaat gtagtaaaac acctaacaag cgacctacta
     6481 agtaagataa actgtagata aaaacaaagg caacacctgc ataataaaca tgactcaatt
     6541 gaaatttgct aatatcttta aaaaagtaaa cttctactcc ttgtacagga ttagaatacg
     6601 gtacccataa cgtaatctga ctagctaatt cttggtaaaa ttgactagca atcatcaaag
     6661 ataccattga cattaacacg taaaagactt gtaaaaaaat acctctactg tagccaatat
     6721 aaaaatgcca aataacaata atcagcaaaa gcaatgatag catgattaat cctcactcac
     6781 tttttcttta atgtccgaaa tcgttttgga gcgtaactta ttgagctcat cttccatttt
     6841 ttcaatgtca atttctcgac ttagttgcac tgataaagca ttaattgcca tgagaatagc
     6901 aatcgtttca ttatcagcat taggtaattt ttctttaata gcttcatact tttctgtagc
     6961 tacacgctca atctcttcca taaaaaggtt gtctttatct gtcgtcaatg tgagaggctt
     7021 atctccaaag acaaatttat aacgattttt ctgactatta tttatttttg tttccatact
     7081 atacctctgc tagcattata ccgtaaaata gggctttcgt aaaattctac cccttatcat
     7141 tacttgtcta ttcccttatt cctctttttt atgatagaat attgtctatg aacacaatcg
     7201 tcatgcaagc agataaaaaa ttgcaagaaa agataagaac cgatttagct caacatcata
     7261 taagtaataa caatccttat gtggtatttt cagctaaaat atcaggagct actgttcttt
     7321 tatatacttc tggaaaactt gtcttccagg gttctaacgc ttcacatatc gcccaaaagt
     7381 atggttttat tgagcaaaaa gaaagttgtt cctctgagag tcaagatatt cccataattg
     7441 gtactgatga agtgggaaat ggctcttact ttggtggact ggcagtggta gctagttttg
     7501 taactcctaa ggaccatgct tacctcaaaa aattaggtgt tggtgattca aaaacactta
     7561 ctgatcaaaa aattaagcaa atcgcgcctc ttttggaaaa agcaattcca cacaaagcat
     7621 tattgttatc gcctcaaaaa tataatcaag tggttagtcc aaataacaaa cataatgctg
     7681 tctccgtaaa agttgcttta cacaatcaag ctatcttcct tcttcttcaa gatggttttg
     7741 aaccagaaaa aatcgttatt gatgctttta ctagctccaa aaactatcaa aattatttga
     7801 aaaatgaaaa gaatcaattt aaacaaacga ttactctgga agaaaaagct gaaaataaat
     7861 atctagctgt agcggtcagt tctattattg cacgtaatct attcttagaa aaccttaaca
     7921 aattaagcga cgacgtagga tataaacttc caagtggagc tggtcaccaa tctgataagg
     7981 tcgctagcca attactcaaa gcttatggta tctcttcgct tgaacactgc gcaaaacttc
     8041 attttgccaa caccaaaaaa gcacaagcat tattaaaata aatagcaggg aaaattatga
     8101 aagaatttat taaagaatgg ggtgtcttta tcctcatcct ctcacttttt ttactatcgc
     8161 gtatcttttt atggcaattc gttaaagttg acggacactc catggatcca actttagctg
     8221 acaaggaaca gctagtagtt ctcaaacaaa caaaaatcaa tcgattcgat attgtagtgg
     8281 ctaacgaaga agaaggcggc caaaagaaaa aaattgttaa acgtgtcatt ggtatgccag
     8341 gtgatgtcat caaatataaa aatgacacct taactattaa caataaaaaa acagaagaac
     8401 cttacctcaa ggaatatact aaattattta aaaaggataa attacaggaa aaatattcgt
     8461 ataacccact tttccaagac ctagcacaaa gctctaccgc tttcaccact gacagcaatg
     8521 gcagcagcga atttactact gtcgtgccta aaggccacta ctatcttgtt ggtgatgacc
     8581 gaattgtctc taaagatagt cgtgccgtcg gtcccttcaa aaaatcaacg attgtgggag
     8641 aggttaaatt ccgcttctgg ccaattcgtc gttttggaac tatcaactaa aggaaattaa
     8701 aaattcacct gacaaaagtt gaaatagcat attatattga aaatagggag tgacaaatcc
     8761 atttaaaata gggatttaca atcactccct ttaactgttt attaaaaagg aaggtacaat
     8821 ggaagtattt tttacaggaa ccattgaacg cattatattt gaaaatgcca gcaatttttt
     8881 taaaatatta cttctggaaa tcgaagatac ggatagcgat tttgatgatg tagaagtaat
     8941 tattactggt actatggccg acgttataga gggagaagaa tatacttttt ggggaacact
     9001 aacccaacat ccaaaatacg gcgagcaact acaaagtgta cgctatgaaa gagccaagcc
     9061 tacttcaggt ggccttgtga aatacttttc tagcgaacaa tttaaaggaa ttggtaaaaa
     9121 gactgcccaa cgcatcgtag aactctatgg agataatacc attgataaaa tccttgaatc
     9181 ccctgaacag ttaagtacaa tctcaggact atcaaaaatt aatagagaag cattcatcgc
     9241 taaactgaaa ctcaattatg gaacagagca agttttagct aaactcgctg aatatgggtt
     9301 aagcaatcgt gctgctattc aaatatttga tcactataaa gaagaatcac ttgaagtgat
     9361 aaacgaaaat ccttatcaac ttgttgagga tatccaaggt atcggtttta aaatagctga
     9421 ccaacttgct gaacaagtcg gtattgaaag tgattctcct aaacgctttc gtgctgctat
     9481 tattcatact ttagttgagt cttctatgga gcaaggagat acctatattg aggctcgtac
     9541 tctcttggaa aaaaccatta ctctccttga agaagctcgt caaatcgaac tagatccttc
     9601 aattgttgct aaggaattga caaatttaat tgcagaagat aaagtgcaac atattggaac
     9661 taaaattttt agtaatactc tattttttgc cgaagaaggc atcaaaaaaa acttacaacg
     9721 aatactaaat cagcctcttg ataaacaact aaatcacaaa gatattgacc gtgaaatcag
     9781 agatattcaa aaatctttaa atatccacta cgataatatc caagaaaaag ctattagaga
     9841 agctttacta agtaaggtat ttatcttgac tggtggccca ggaacaggta aaacaacagt
     9901 tataaatggt attattgaag cctattccga gttacatcat attgatttaa acaaaaatga
     9961 tattcctatt gtacttgctg ctcctacagg tcgtgctgct agacgcatga atgaacttac
    10021 cggtttaccg agtgctacta ttcataggca tttgggatta aacggagata gtgactatca
    10081 aagtctagat gactatctag attgttcctt aattatcatt gatgaatttt caatggttga
    10141 cacttggctt gctaatcaat tatttgacgc tttggactcg cacacacaag ttattattgt
    10201 cggagatagt gatcaattac cgtctgtagg tccaggacaa gttctagcag atcttttaaa
    10261 tataaatgca cttccacacg ttaaacttga aaagattttt agacaatcag aagaatcaac
    10321 tattgtgact ttagctaatc aaatgagaca gggctttttg ccagaggact ttacagctaa
    10381 aaaagccgat cgctcatact ttgaagcatc tgctaatatc atcccaaata tgatttctaa
    10441 aattgttcaa tctgcactta agagcggtat tgaagctcat gaaattcaaa ttttagctcc
    10501 tatgtatcgc ggccaagcag gtattaataa tcttaacctt atcatgcaaa atctcctcaa
    10561 tccattaaaa gataataacc aatttacttt taatgatata aatttccgaa taggagataa
    10621 agtgttacat ttagtcaacg atacagagct taatgttttt aatggggata ttggttacat
    10681 aacggacctt ataccagcca aatatactga gagcaagcaa gatgagattt atatgacttt
    10741 tgacggtcaa gaagttatct atcaacgcaa agaatggctt aaaataacac tagcctatgc
    10801 tatgagtatt cataaatcac agggttctga atttcaagtt gttattctgc caataacgag
    10861 acagagtggt cgaatgttac agcgaaacct tatttataca gctattacga gatctaaaag
    10921 taaactaata cttttaggag aaattggtgc atttgatttc gctgttaaaa acgagggggc
    10981 aaagcgcaat acttatctga tagaaagatt tgaaaacaag caagaaatag ctaattctca
    11041 aaaaattgaa gatagctcaa tagatcaaaa gattgacaac acaataatta atacgtccat
    11101 tccaaaaaca gcaacaccaa ttgagcagac taatctttca aaaataactt atcgcctaac
    11161 tgaagaaaat tatttaacaa tcgatccaat gattggtatt aatcaacaag atataagtgc
    11221 aatttttgat agtaaataaa ttttttcata aaatcttgaa aactctt
//
LOCUS       DATRSD010000065        11255 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRSD010000065 DATRSD010000000
VERSION     DATRSD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.066"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..180
                     /locus_tag="VBD85_001506"
     CDS             <1..180
                     /locus_tag="VBD85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134055.1"
                     /translation="KNLLQESINKRLSEERYQIPKEKREKKRFDMQLILIISILIGLI
                     MSIIGIIRFFLTYSS"
     gene            264..717
                     /locus_tag="VBD85_001507"
                     /pseudo
     CDS             264..717
                     /locus_tag="VBD85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(762..2234)
                     /locus_tag="VBD85_001508"
     CDS             complement(762..2234)
                     /locus_tag="VBD85_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7134056.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQTLYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(2465..3301)
                     /locus_tag="VBD85_001509"
     CDS             complement(2465..3301)
                     /locus_tag="VBD85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7134057.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(3301..4671)
                     /locus_tag="VBD85_001510"
     CDS             complement(3301..4671)
                     /locus_tag="VBD85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7134058.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4769..6016)
                     /locus_tag="VBD85_001511"
     CDS             complement(4769..6016)
                     /locus_tag="VBD85_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7134059.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            6272..7300
                     /locus_tag="VBD85_001512"
     CDS             6272..7300
                     /locus_tag="VBD85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7134060.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNASISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            7421..8917
                     /gene="malQ"
                     /locus_tag="VBD85_001513"
     CDS             7421..8917
                     /gene="malQ"
                     /locus_tag="VBD85_001513"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO7134061.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDRDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8929..11193
                     /gene="glgP"
                     /locus_tag="VBD85_001514"
     CDS             8929..11193
                     /gene="glgP"
                     /locus_tag="VBD85_001514"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO7134062.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3855 a         1787 c         2110 g         3503 t
ORIGIN      
        1 aagaatttac ttcaggagag tattaataaa cgtctatctg aagagcgata tcaaattcct
       61 aaagaaaaaa gagagaaaaa acgatttgac atgcagctta ttctgattat ttctatttta
      121 atagggttaa tcatgtctat tatcggaatt attcgatttt tcttaaccta cagtagttaa
      181 gttactatcc tttattttgt agaaaaattg ataaataaat ggtattgtaa ctctatcact
      241 aaatttatat actagaggaa aaaatgtcac gcagtcaaaa agttatttta actaatatgt
      301 gtcttattga agataataaa ggacgtattg tgatgcaaat ccgtgatcct aaacgttatt
      361 cttggtcagg agctgcttta ccaggcggcc atattgaaga aggagaatgc tctcgttcga
      421 gaagttcacg aggagacagg tttaacaata agtaatccca aattagttgg tatgaagcat
      481 tggtacacag atgaaggcat ccgctatttg gtattccttt ataaaacata tgatttttca
      541 ggtgaattac aatcgtcaga tgaaggtgat gttgtatgga ttgaacgttc acaacttgat
      601 aagattgaac tagcatatga tatgcttaac cttttgagag tatttgacga agatgacatc
      661 tcagagatgt tttatagtaa gcgacttgaa gatggtttta taagaaaatt ttattaaaca
      721 aaaaagaact aggaatgtaa cctagttctt tttagatgtc tttatttaac tccagacatt
      781 agtttttcaa tgcgtggaac ataaaataag aggataaatc caaatacaac tgttatacca
      841 ccaacaacac cataataaag agtttgagta tcaggtgtgt acaatttaac gatttgagcg
      901 ttaatagctt gtgctgcagc attacttaag aaccagatgc tcatcatttg agcttggaat
      961 gctttaggtg cgagcttact agttgctgat aatccaactg gtgagattag catttcccca
     1021 acgatgacaa tagcccaact cattgttaac caaagagggc ttactttagc gttaactcca
     1081 aagagtaaac ctggtagcat catccataag aatgaagctc cagcaaagaa aagtccatac
     1141 gcaaattttt taggtgatga aggttgctta cttccccatt tagcccataa ccaagcaaag
     1201 aatggtacat aaagcataat gaagagaggg ttcatacttt ggaaataact tgatgggaaa
     1261 ttaatatgat gcccaaagaa gttaaggtaa agttttgttt gttcatctgc aaataaggct
     1321 aagacaactg aaccttgttc ttcaattgac caaaatagaa ttgaggcgat aaagagaggg
     1381 atatatgccc acactcgaga acgctcaaca gaagaaatct ttttactact taaaatcttg
     1441 ataaaataaa taattgggat ggcgattgca ataatactaa aaatatcgat aattacttca
     1501 attgttaaca tatgcatcaa gtgaagtaga ataagtacaa taacaatacc aataaggata
     1561 agcccaactt ttttatatag agaggacttt tcttcaggac ttaaaggatc gtttggtctg
     1621 agactctctt cagtcaaata tttttttcca tctaaggtat attggaggag accaaagaac
     1681 ataccaatag cagcaagtga gaaaccaaga tggaaattta cttcttgtcc gaggtacccc
     1741 actacaatgg gtgaaataaa agcaccaagg ttaataccaa aaacaaagat actaaaacca
     1801 gcatcacgac gagaatcatt ttctccatat aaattaccaa ccatctctga tacatttggt
     1861 tttaatagtc cagtaccaag gataattaga gcaatcgaaa tgaatagagc tgtttgacca
     1921 aatggtgtag ctagagcaat atgacctagc atgattagaa taccaccata cagaacagtt
     1981 ttacgactac ctagaatacg gtcgctgaca aaaccaccaa ttactgatga gagataaacc
     2041 agtgaaccat agacagccat gattgatgca gcgacagtct tgtccatacc aaggccacct
     2101 tgagaaacac tatagtacat atagtacagc aaaatagcac gcataccata gtatgagaat
     2161 ctttcccaca tttcagtaaa gaaaagagtg gacaaaccac gaggatgtcc aaagaatgat
     2221 tttgtttttt ccatgaaaaa cgctcctttt gtagataaca ctattaataa tatagtaaat
     2281 agaagcaact gtcaaattac ttagcactat caagttattt ttttgtagag gttaacaccc
     2341 ttggtaaacc aagtttttta actatctagc ttagaaaaag gcttgaattc aatagaaccc
     2401 aagccttttt tagaaaagaa accacagtga aataaaaatg tcgtgtcttt aaattatctg
     2461 aagattattt tacagatcca cctgtaatac cttcaacata gtatttttgc atgaacatga
     2521 aaagtattgt gattggaatt gcaataataa cagatcctgc tgtaaatgac atgaaccact
     2581 ggttgatggt atcttgttgc aacattgaaa agagaccaat agcgactgtg tacttgctcg
     2641 tagcatcacc taaaataact tttgcaaaga taaagtccat ccaagggccc ataaaagcga
     2701 ttaatgcagt ataaacgata atgggttttg acaatggtag ggtgatttta aggaaaatat
     2761 ccaaacgggt cgccccatca atcatagcag actcatccaa agagtatgga atagtatcaa
     2821 agaaaccttt tgcgatgtag aaggttaatg ctgctcctgc agaatagaca aatattaaag
     2881 cggttaatgt ttggtctaga tttagagctt ttaggatgta gtaaactgca atcattgaca
     2941 tgaacccagg aaacatattc aagactaaag ctagctttag gaaaccatta cggtgtttga
     3001 atttgattct gctgagagaa taagccattg ctacagtgat taaagttgat agtatacaag
     3061 tacatgtagc gacaaataat gtatttaaga accattgacc aaatgggaat gtgttttggg
     3121 tgaagagttt agcgtagttg tctaatgtcc aagttttagg aataaagtag ttgacaaagg
     3181 cacttccttc accacggaag cttgtgagaa cgacccaaac aatgggaaag agccacataa
     3241 ttgatagtac tatcaataaa atataaacaa aagtaaggtt aagacgtttt ttcttattca
     3301 ttatttagct gccccttcct tgtaagaatt tgttcttgtg taagctagta aactaaatac
     3361 tgctgaaaga ataaagatta agataccaac aacagatgct aagttataat ctgcagcagt
     3421 tactgtcaaa ttgtatagcc aagtgacaag taaatcggtg ctacctgctt ggtagtatgt
     3481 tgagtttgtt ggtccacctt gagtgagtag gtagataacg ttgaagttgt taatatttcc
     3541 gataaattgt tgaattaaag taggagtcat aatcaacaaa atttgtggga aagtaattga
     3601 ttgaaagact tggaacttat tcgccccatc aatttcagca gcttcaattt gttctgcagg
     3661 taaattcatg atgataccag tagcaacaag cattgttaca ggaataccaa cccacatatt
     3721 aacaaagatg attgagaatt tcgcccagac cggatcagaa aggaatggaa ggggatgtgc
     3781 tgaagaaata agtccccatt ttattaacaa ggcgttaacc ggtccagcgt cacttaagag
     3841 gtttctcatg attaagagag agataaattg agggacagcc atggtaataa caaaaatagt
     3901 acgccacatc tttttgaatt tcaaaccttt tgtattaatt agtaaggcaa gaataatacc
     3961 aaagaagaaa ttcgttacag tagcgaaaac agcccaaatt aatgtccaag atagaacagg
     4021 gaagaaagta ctggccatac gcccgctaaa gatattaccg aagttagcaa aaccaaccca
     4081 gtcaaataaa ctttttggcg gtaagtggtt atgatcatag ttagtaaatg ccaaacaaat
     4141 catataaact aacggaagga ttgtaaatag aagcacccct attaaaggaa tagacattaa
     4201 agtcatatgg aagcgtccat ctgtcaagct tttaatatct tttttgaagg atggaatttc
     4261 tcttccttct tctttcaagg tcaataaatg tgctgcgctt ttaatgtttg accagtaaat
     4321 ataggcaaat acgcaacaga agataagcga agctaatcca aaaattagca ttaacataga
     4381 gttatcacct tgtgtcgcaa cttggatttt aataccatcc acagttttag ttgtcatacc
     4441 ttgttcttgg gtccctaagg aaataagacc ttttacagct gggataactt ggtaaacaaa
     4501 ggtaattaaa aacagaattt cactgattaa aaataaaagt cccttgacaa tttgtttatt
     4561 tttgaggtta gcaagtccca taattaagaa tgatagttta gttgctaaat cacctttttg
     4621 aaagacctct tttagggaca taccatcaag tgagatcagt tttttattca taatggtctc
     4681 ctttaattgt tattttacaa aataagagat gggaagcact ctcaattgaa aggaagagta
     4741 ctcacctcat ctcattactc tagacgtact attttgtttt agcgatgtct ttatcgaatt
     4801 gttttagacg tttaaggtaa tcgctagatt tgattttacc actgtaagta tcgctaagaa
     4861 tagcagcact ttctgtccag aaggttgaca tttgactcaa cttaggcata accgttgtat
     4921 atttatctga gctacccatt tcgataactg cttttgcaag ttcatctttt tggacgctat
     4981 cagaagattg aattgatgag ttagcaggaa cgatatgacg tttttcgaat tgaatttttt
     5041 gactttcagc attagttaga tatgcagcga gtttgtagct agctgagatt cgtttagtgt
     5101 ttgaaccagc aggtgcttgg ttaacggcat aaagtttaac gcccaagaat gctttttgtt
     5161 gaacttcttt gtcaccgatt ttcattgttg ggtaaacagc aacaccgatt ttatcttcac
     5221 cgacagcttt tttagcagcg tcgtaatccc atggtccact ttcaaaagca tgaacagaac
     5281 catcgccaaa tttagacatt gtattttcag ctgtcaagtt gacaaaacca tcatttttct
     5341 tttgatctgc aatccattta aggacagaaa cacctgcttc attaccccag ttagtgcctt
     5401 tagcatcttc accagattta ccaaataaag tgtcgcctac agaaaggaaa agaggacctg
     5461 ttacatatga gttagctgct ttaagctgtt gaccgaattt ccctttgctt gtaattgttt
     5521 cgtatgattt aacgtcgtca gcagttaact ttgttttatt ataataaaga acttgagatt
     5581 caataccaaa tgggaatgca taagttttcc ctttatattg tgcaccagta agtgattgtt
     5641 tagtgtcgtt tttagcaatt tcttttgagt attgctctgg aatttcttgg ataacaccag
     5701 attctactaa ttgaccaagt tggtcatgtg gaagtgagaa tacatcggct gccttgcttg
     5761 ggtctttttt tacgttttct tgagctttgg agtcattaga ctcaatcatt tttacagtaa
     5821 cgcctttgtt ttccttctcg aattttttaa caattgcttt ataagacgct tttgaatctg
     5881 ttgggaccca aagtttgata gttgtttttg aagcagcatc aactgagtta gagtgagtag
     5941 cagcaattgc tcctcctgca actactgaaa gagcagcagt actaacaagt aattttttcc
     6001 aagtattttt ttccattttg gttctcctcc tagagaatat tttaattaca cctatattat
     6061 gcaaccgttt gcaaaaaaag tcaagtcttt ttttaaaaaa aataagaaaa ataaaaaaat
     6121 aatatcaatt aatactaaat gagaagatta ttttagtgag agtattacat ttgcagagaa
     6181 ggcaaacgtt tgtttttttc ttagattaga gtataataga caaaggacta taccaaaagg
     6241 tataaaatga ttagaatgaa gaggtcgtag gatggtaact ataaaagatg ttgctgccaa
     6301 agctggtgtt aatccatcga cagtaagtcg tgtactaaaa gataatgcat ctatttcaag
     6361 taagacgaaa gagcgcgtga aaaaagctat ggaagagctg ggctatgttc ctaatgttgc
     6421 agcacaaatg ttggctagtg gtttaacgca aaatattgga gttatttttc ctccactggt
     6481 tttaccagac cgcttaagtg aacccttttt catggagatt ctttctacat taactaatga
     6541 agcaaaagag catgggttta cagtttctat tgcaacaagt atgtcttctg aggagttgtt
     6601 agaacaagta aaattaatgc atctacaaaa acgagtggat ggttttattg ttctctattc
     6661 ggatcaaaaa gatcctatca gagagtattt attaaaatat aaactgccct ttgttgttgt
     6721 tggtgctgct aatggctatg aaaatgagat tacttatatt gataatgata atcagctgat
     6781 ggggaaatct gccattgatt atctctataa aaaaggacat aaaagcatcc agtttgttac
     6841 agatgattta aattcagaag tatcagaaga gcgctattta gggtatttta aaggtgctag
     6901 aaagttaggt ttaaaccaaa agccagctct tttatttgac agaggcaatc cgcaagtttt
     6961 agaggaattt ataaatagag tcaaagaaga agaaacaact gctttaattg ttattggtga
     7021 tactgtctct gttagagtaa tgcaatttct ttctttctat aaattaaaag ttccagatga
     7081 catttctatc atgaccttta ataattctct cttctcacat ctgattcatc cttacctaag
     7141 cacatttgat attaatgtta ataatctagg aaggacaagc gttcgtcgtc tgattgacat
     7201 cattaaatct ccagacaaag tctttagtga gactattatt gtgcctttta ctttagaaga
     7261 gcgtgaaagt gtccgagata tttctaaaaa taataattaa gctatgatat aaagacttct
     7321 atcaattttc gatagaagtt tttttaaatt atgcttgact tatgcaatcg cttgcgttaa
     7381 gatggaggag taaagtaagt aatcgaaaag gagaatcaat atgaaaaaac gtgcaagtgg
     7441 tgtcttaatg cacatcactt ctctaccagg tgatttaggt attggaacat ttggacgaga
     7501 agcctacgct tttgttgatt ttttggtcga aactgatcaa aaattttggc aaattcttcc
     7561 cttaacaaca acaagcttcg gggactctcc ttaccagtct ttttctgcag tagctggtaa
     7621 tacacattta attgattttg atttgctaac gcttgaaggt ttcatttcga aagatgacta
     7681 ccaaaatatt agttttggac aggatccaga agttgttgat tatgctggtc tgtttgaaaa
     7741 acgccgtcca gttttagaaa aagcagttaa aaatttcttg caagaagaga gagctacgag
     7801 aatgctatct gatttcttgc aagaagaaaa atgggtaact gattttgctg aatttatggc
     7861 gatcaaagaa cattttggta ataaggcgct tcaagaatgg gatgacaagg ctattatacg
     7921 ccgcgaagaa gaagccttag caggatatcg tcaaaagctt agtgaagtga taaaatatca
     7981 tgaagtaacg caatatttct tttacaaaca atggtttgag ttaaaagaat atgctaatga
     8041 taaagggatt caaattatcg gtgatatgcc aatctacgtt tctgccgata gtgtagaagt
     8101 ttggacaatg cctgaactgt ttaaattaga tcgcgacaaa caaccacttg ctattgctgg
     8161 tgtaccagcg gatgattttt ctgacgatgg tcagctttgg ggcaatccta tttacaattg
     8221 ggattaccac aaagagtcag attttgactg gtggatttat cgtattcaat ctggtgttaa
     8281 gatgtatgac tatttgcgta ttgatcattt taaaggtttt tcagattatt gggaaattcg
     8341 aggagattat caaacagcta atgatggctc atggcaacca gctcccggtc cagaattatt
     8401 tgcaactatc aaagaaaaat taggtgattt acctattatt gctgagaatt taggctatat
     8461 tgatgagaga gcagagagat tactggctgg aacagggttc ccaggtatga aaatcatgga
     8521 attcggtttt tatgatacaa caggaaatag tattgatatt cctcataact acacagaaaa
     8581 tacaattgca tatgctggaa ctcatgataa cgaagttatt aatggctggt ttgaaaatct
     8641 tacagtcgaa cagaaagctt atgcagaaaa ttacatgcgt cgtttgccaa atgagcccat
     8701 tactgagact gtactgcgta ccttgtatgc aaccgttagt caaacaacga ttacatgtat
     8761 gcaagatcta ctagataaac ctgcagatag ccgtatgaac atgccaaata ccgttggagg
     8821 taactggcaa tggcgtatga gaaaagaaga cttaacagaa aatcgcaaag cattcttaaa
     8881 agagattaca actatttata atagagggaa taaataagga atattatcat gacaagaaat
     8941 tttacaactt acgttggaca acaaggaaaa gtattatcag aattaacaaa tgaagagatt
     9001 tacgttgaat tgttaaattt tgtaaaagaa gaagctgccg ctaaatcaaa aaatagctct
     9061 caacgtaagg tatactatat ttcagctgag tttctaattg gtaaattact ttcaaataat
     9121 ttgattaact tagggattta taaagatgtc aaaaaagagt tagaacttgt aggtaaatca
     9181 attgctgaaa tagaagatgt agagccagaa ccatcacttg gtaatggtgg tttagggcgt
     9241 ttagcatcat gttttattga ctcgatttct tctttaggta ttaatggtga aggtgttggt
     9301 ttaaactatc actgtgggct tttcaaacaa gtatttcgca acaatcaaca agaagcagaa
     9361 gctaactact ggattgagaa taattcttgg ttagtaccaa cagatatttc ttatgatgtt
     9421 cctttccgtg actttacttt aaaatcacgc ctagatagaa ttgatgtatt aggttataag
     9481 aaagatacta aaaattattt gaaccttttt gatattgatg gacttgatta taaccttatt
     9541 gaaaaaggta tcacttttga taagacagaa atcaagaaga atttaacctt gttcttgtat
     9601 ccagatgatt cagataaaaa tggagaatta ttacgtatct atcagcaata tttcatggtt
     9661 tcaaatgctg ctcaattatt aattgatgaa gcaatcgagc gtggttcaaa ccttcatgat
     9721 ttagctgaat atgcttatgt tcaaattaat gacactcacc catctatggt catccctgag
     9781 ttgattcgtt tattgacaga aaaacatggt tttgaatttg acgaagctgt gtcagttgtt
     9841 cgcaatatgg ttggctatac aaaccatacc atccttgcag aagctcttga aaaatggcct
     9901 ttagagtatc ttaatgaggt agtaccccac ctagtaacaa tcattaaaaa attagatcaa
     9961 atgattcgtg aagagcaaac aaatccagag gttcaaatta ttgatgaagc tggacgtgtt
    10021 cacatggcgc atatggatat ccacttctca acaagcgtta atggtgtagc agcattacat
    10081 acagaaatcc ttaaaaatag tgaattaaag gttttctatg atatttaccc agataagttt
    10141 aacaataaga caaacggtat tacattccgt cgctggttgg agtttgctaa ccaagattta
    10201 gcagattact tgaaagaact aattggggat agctatctta ctgatgcgac tcagctcgaa
    10261 aaattgttaa cttatgctga tagcaatgaa gttcatgata aattagctgc tattaaattt
    10321 aaaaacaaac tggctcttaa acgttacctt aaggaaaata aaggtattga attggatgaa
    10381 tactcaatta ttgatacaca aatcaaacga ttccacgaat ataagcgcca acaaatgaat
    10441 gctttatatg ttattcataa atacttagaa attaaacgtg ggcatttccc atcacgtaaa
    10501 ttaacagtta tttttggtgg taaagctgct ccagcctata ctattgcaca ggatatcatt
    10561 cacttaattc tctgcttatc agaattaatt aataatgatc cagaagttaa taaatacctt
    10621 aatgtccatt tagtagaaaa ttacaatgtg acagtagcgg aaaagttaat ccctgcaaca
    10681 gatatttcgg aacaaatttc attggcttcc aaagaagcat ctggtacagg taacatgaaa
    10741 tttatgttga atggtgctct tactcttggt actatggatg gggcaaatgt tgaaatagct
    10801 gaattagctg gcaaagagaa catctacaca tttggtaaag actctgatac tatcattaat
    10861 ctttatgaaa catctggtta tcgttcaaaa gattactatg ataaagacaa agtaattcgt
    10921 gaagctgttg attttatcat tagtgatgat atagtgtcac tcggtaatgc agaacgacta
    10981 aagagattgc atgatgagtt agtaggtaaa gattggttca tgacattgat tgatttgaaa
    11041 gaatacattg ctgttaaaga acaagtgctt gctgattatg aagattatga atcatggaat
    11101 aagaaagtta tacacaatat tgctaaagca gggttcttct catcagatcg tacaatcgaa
    11161 caatataatc aagatatttg gcattcaaat taaaaagtct ctaaaagtaa ggttttcaac
    11221 cttacttttt tcttgtctaa taaaactata agtat
//
LOCUS       DATRSD010000066        11201 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRSD010000066 DATRSD010000000
VERSION     DATRSD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.067"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..923)
                     /locus_tag="VBD85_001515"
     CDS             complement(<1..923)
                     /locus_tag="VBD85_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO7134063.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLMIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANAA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLS"
     gene            complement(1110..2477)
                     /locus_tag="VBD85_001516"
     CDS             complement(1110..2477)
                     /locus_tag="VBD85_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO7134064.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIILEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(2623..3669)
                     /locus_tag="VBD85_001517"
     CDS             complement(2623..3669)
                     /locus_tag="VBD85_001517"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO7134065.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(3810..4406)
                     /gene="recR"
                     /locus_tag="VBD85_001518"
     CDS             complement(3810..4406)
                     /gene="recR"
                     /locus_tag="VBD85_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO7134066.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4421..6466)
                     /locus_tag="VBD85_001519"
     CDS             complement(4421..6466)
                     /locus_tag="VBD85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO7134067.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     VNGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(6595..7287)
                     /locus_tag="VBD85_001520"
     CDS             complement(6595..7287)
                     /locus_tag="VBD85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO7134068.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(7464..8222)
                     /locus_tag="VBD85_001521"
     CDS             complement(7464..8222)
                     /locus_tag="VBD85_001521"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO7134069.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(8403..9599)
                     /gene="tuf"
                     /locus_tag="VBD85_001522"
     CDS             complement(8403..9599)
                     /gene="tuf"
                     /locus_tag="VBD85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO7134070.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(9951..>11201)
                     /locus_tag="VBD85_001523"
     CDS             complement(9951..>11201)
                     /locus_tag="VBD85_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7134071.1"
                     /translation="HLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPFRSVINQ
                     GVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGAHGWIVI
                     GPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLKDWRFYS
                     LFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFIGTIAVV
                     GVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGLGNSIEK
                     LGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNSMIALGI
                     GAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDANEKKELI
                     MKEAEEQYKPQEKNEKIINLDAFK"
BASE COUNT         3532 a         2244 c         1786 g         3639 t
ORIGIN      
        1 gacaaaaata aactagaagt cataataaag ttcacaataa ataatattat aaaggtcaac
       61 gggcgtccaa tgtagtcaga gagacttgcc cataacagac gtccaaaacc gttaaatatc
      121 cctagtaccc ctactaataa ggctgcagat tctgcggaat agcctgctaa atcttgtgcc
      181 attggtgaag ctgctgatat taaacctaag ccacacgaaa tattaataaa caaagtcaac
      241 caaatgatgt aaaaggtttt tgattttata gcagcatttg cttttaatcc agtaataatt
      301 tgtgaattca tagcattctt tttaccgtcg tgagtcaaaa tagttatttt ttcctgaggt
      361 ggttgtttaa taaattgtga ggcaatcatc atgacaaaaa aatatactaa tcccaaaata
      421 taaaatgttt tacctacacc aatcctaatc attaaggatt gtgcaagcgg acttgttact
      481 aaagaagcaa agccaaatcc cataatagcg aatcctgttg ctagtcccct cctatcagga
      541 aaccatttaa taatagtcga tactggagta atataacctg aaccaagtcc gattcctcct
      601 aaaataccgt atgcaacata cagtaaccat aactgctgag tttcaatggc taaacctgtt
      661 aacacattcc ctgctccata taaaatagca gaaatcattc ccattatcct aggaccaaaa
      721 cgctctacta agtgtcccat aaaagctgca gacattccta gacaaaaaat agccaaacta
      781 aaagcgaatg aaactgatga aatatcccaa ccagtctctg agatgattgg gttacgaaac
      841 acactccaag cataagttga tcctagcatt aaatgtaaaa cgactccaga aaccgcaact
      901 acataacgat ttaaattttt caaacaatcc tcctaaaaac gaacttaagt tttattttta
      961 aaaatatagt tcgtaataaa cagattatca aaaaactgaa tatatcacca gttttctgct
     1021 tagcatatct attttcctca ataaaactga atattattcg tgattaaaaa acaaaaaagt
     1081 tagggtaact tgttacccta acacctctat tatcctactt caagaatatc aactatttta
     1141 gaaagattca ttgagttaga accttttaat aatatttgat ctttctcctt aagagtatct
     1201 tttacttttg caagcaattg atcaaactga tctacttctt gattcttttt aaagaaatac
     1261 actttaccaa ttgggaacat ctggcttgct aattgagcca acccctcaat atcctggcca
     1321 taacaaatca aagtatcaat agagtcaggt ctgatactca taatcatttg attatgtaaa
     1381 tccacagatt gttctcccag ttctttcata tcagctaaaa gagcaatctt ctttccacca
     1441 tcattattgg gaatagctga aaaggtttct agtatcaatc gcatagccgt tgggttagca
     1501 ttgtaaacat ctgataaaat atctgctcca ttggctgatt tcttccattc ggtgcgatta
     1561 cgagttagtt gaagattttc tagagcatcc actatatctt cttctgctac tgctaataat
     1621 tttccaacat aggcagcgac catcgcatta gttgcattgt acttaccagg tactggtata
     1681 cgtaattggt gctccaaagc attagtttta aaagttaagg aatgtttttc ttctttgagt
     1741 tctgtcactt gcaattcttg gtcatgacca aaacgaatag tcatctggtt tgctggaaga
     1801 tatggatcaa taattggatc tcccggtgca attaatatgc catctgaact cataccatct
     1861 gtgatttgca ttttcccttc tgcaatcttt tctcgactac caaaaaattc taagtgtgct
     1921 tcaccaatca aagtaacaac agcaatacgt ggttttgcaa tttcagataa cacatggata
     1981 tctccaagat gatcctgtcc catctccaaa ataatttttt cagtatcttc tggcatgtgt
     2041 agcacagtat agggtaagcc aatctcgtta ttgtagtttc cttgtgtttt ataagtctta
     2101 tatgtggttg ataagattgc agctatcata tctttagttg ttgtcttacc atttgagcca
     2161 gttactgcaa taacatcaac gttcatcttt tcaatataat actgtgccaa gacttgaaat
     2221 gctttaagtg catcagacac taaaagataa ggatgaccct ctatttcctt ctctgaaatg
     2281 gttgctattg ctccattatc aaaagccatc tctataaact catgtccatc tcttgctcct
     2341 tttaagggaa gaaataagtc accttcactg atattacgac tatcaaattc aatatttccc
     2401 aggggaacgt cttcaaactc tgatacttga tttttagcgc caactacttt agcaacttca
     2461 tgcaaactaa gcttcatatc atttcctttt ctattattta gcaacaatgt tgtagatagt
     2521 atgctatcct tataaacgaa tatactccta aattataaca cactttcata acatatcata
     2581 gatttgaaaa agctcaacta agaaagactt agttgaattt ttttaaatta aatgactctc
     2641 acgtttttca aacatttctt tagctaacat aactaacttt tcaatcaaat cactataagt
     2701 tagccccata ttttcccaaa gcaggggata cattgaccac tgagtaaaac cgggcattgt
     2761 attcagttcg tttaagaaga tttgtccatc tttcgtcaaa aagaaatcac agcgtgataa
     2821 accacaagcc ccgattgctt taaaagcttt acttgcatat tgacgcattg cttccatagt
     2881 tgcttcatca actttagctg gaatatccat agtaatttta ttatcaatat atttggcgtc
     2941 atagtcatag aaatcgacgt ctttaacgac ttcgccagga aaagttgtct taacatcatt
     3001 attgcctaaa atacctactt caatttcacg agctgtcacg ccttgttcaa tcaaaatacg
     3061 gctatcatac ttgagagcta agtcaattgc agagcgaagt gaggattcat ctgtcgcttt
     3121 tgaaatacct actgatgacc ccatattagc cggttttaca aaaattggga aacttaaagt
     3181 ttctaaagag agtttaatcg catgttccaa atcatcaccc tcaaaataag tttgatatgc
     3241 aacctgaggt acacctactg ttgcaaggac ttgttttgtt gtaattttat ccatagccac
     3301 gcttgaagat agaatattag tcccaacata aggcatcctt aaaacttcta aaaatccttg
     3361 gatagaacca tcttccccca ttggtccatg taaaacgggg aaaacaattg cattatcatc
     3421 atagatatca cttggacgaa ccattttgtc taaatcaaca gtttggtttg tcattaactt
     3481 ttcatctgaa gatggcattt catcaaattc ttgtgtttta ataaattgac ctacttgcgt
     3541 gataaaataa gttttaacaa aaaatttatc ataattaata gcacgcatga cgctttctgc
     3601 agacagtaca gatacttcac gttccgcaga gcgaccaccg taaagcaaga tcaatgtttc
     3661 ttttgacata tttcttctct tttctatcta atctaagatt gaccttttaa taataccata
     3721 ttttaaccta actttcaaaa atcaatccac ttaaagttag tattgtaaaa gaaaaagtta
     3781 ggagattccc aaccttttct ttataacaat tataactctg tacgattttc gattgctcga
     3841 agcaaggtta cttcatctgc atattcgata tctgaaccaa cagctaaacc tctcgctaat
     3901 cgtgtcactt tgataccagc tggttttaag actcgtgaaa tatacattga tgtagcttca
     3961 ccatcagcag tcgcattggt agcaacaata acttctgtca cctgaccatc catcaatcga
     4021 gtaattaaac ttttcaaatt aatgtcatca gggctaatac cattcatagg tgaaattaag
     4081 ccatgtaaga catgatacaa accattatac tcttgaattt tttccatagc tgacacatct
     4141 ttactatctt caacaaccaa gataacagac tggtcacgcg tcttatctgt acaaattaag
     4201 caaggatcat catcagtcaa attaccacaa acagaacaat aggttaattc ccgcttagca
     4261 gctaataaat tcttagcaaa ttcattaaca tcttcatcac tcataccaat ggtgtaaaaa
     4321 gctaaacgtg ttgctgtttt agtacctata cccggtaatt ttgaaaagct gtcaatcaat
     4381 ttagcaattg gtgttgggta aagcattcaa atgtcctctc ttattgtcct gtgaactgtg
     4441 aaatatattg atcaatcata tctcgtgcaa ctaattgatg ggattttgtt gtatcagttg
     4501 taacatgcgg atacattacc gctactgcta ttttacgatt agtatcgtag gcaatagcgt
     4561 ttaagttgta ggtagaaact gtttgtccat ttacattttt agcaaatgtt tcagcggtac
     4621 cagttttacc actaatggtt gtcacattcc ccctcattga cgtaccagtt gcatagccac
     4681 ttccactatt gacaacgtta taaaaacctt cttgaataat ggctaactct tgatctgaga
     4741 tagctatctt atttagtgtt ttaggagtga ttgaacgaac caattgagcg aacttattag
     4801 aatcattccc ttcatagata tctgaaacca cgtgaggtgc taaacgattc ccattattcg
     4861 caatagttga gatatactga ccaagttgca ttggtgtata ggcatcatac tgaccgaacg
     4921 attccattag agttgttcct aagctgtatt ttccaggtac ataaccttct gattcaggta
     4981 aatctaatcc tgtagaaacc cctaaaccat attcgccgta agttttcctt agtttagcca
     5041 ttgcttcttg atagcctcga tcagttaaag catctcctgt attatagtct tgtcccatta
     5101 aacgaagtgc tacttgaacc atataagtat tagatgaata ctctagcgct tgtgccgcag
     5161 atattggagt caaacctcgt gtaaaccatg accgtatatt agcaatttct tggtcataaa
     5221 gcacttcatt tcctctaaga actttatttt cccatcctga tgatagcgtc gcacctttca
     5281 caacagatcc tggcgtgaaa ttcttagcaa tagtgcctag gctgtcatca actaaattcc
     5341 catgatcatt tttcagacca gccattgcta acactttacc agttgaaggt tctatagcaa
     5401 ctgcatacat cccttcggaa aaactagcgc gtccactaga taattcactg ccatagtatt
     5461 gtttcaagat actttctact ttgttttggt aattaagatc aatcgttaat ttaagatttc
     5521 ttcctgactt accttcttgc gtaatattat ctgaaacaac tttacctttt ttattaacaa
     5581 ctactttgcg aatttgtctg attccttgta agtcatcttc atattgtttt tcaaggtaag
     5641 aagttcccac tcggtcattc agtgaatagc cttttttcaa atatttttta acttcttctc
     5701 ttggtaaacc tgccttttct gtagaaatag tacctaatat atccgataaa gaagttcctt
     5761 ttttacgacg atgccaatta cttgttacag aaattcctaa cagttccttc ttatcagcat
     5821 ccagtttctt gatttgatca tcacttaatt cgcctgtact taatttcact gaaccaaacg
     5881 ttggtgtcgc attcatttgg ttaaataagg caaccacttt taactcatct tcagaatagt
     5941 taatagcgct cactggaact gcagcaactg catttgcata tacggttgat tcggctagat
     6001 ggttaccaaa tttatcgtaa cgtttagaat ctggtaaact ttcaacaact tttttgtagt
     6061 tggctttatc tgctaaataa taatctctct ttgctctatc gcttgaagca acttcggtta
     6121 aggtaatgta atgtgataat ttattcgcta actctttgat agactgagat gaaatattgt
     6181 ttcctctaga aaacgccaca gtaggacgtt catcattttc aactaagaca actcccttag
     6241 catcaaaaat ctgacctctc gcttttgatt ctttcactgt ataactcgta agagctgtca
     6301 gttttttcat gtaaaatgat tgttgcccaa tctgcatttg ctctaaacga agaattagaa
     6361 cagtaaataa taaaacaata ataaaaaata gaagatttaa tcgtctaggt atgctagcat
     6421 tttgcttttt tacagtgaga cgataccttt ttttacgatt caacattaac tccttgctat
     6481 aaaaactctg actactattg tatcataaaa atggtttttt acagacactc aaaatgttat
     6541 aaataataaa aaattcaagc caacttggcc tgaatttttt atataaagat tagattattt
     6601 acctaagtaa tattctgaaa caaggtttaa tttttcatca aattcgaaaa caagtggtgg
     6661 gaagttagga atttcaacgt ccatgatttc atcatctgac aattgtttga tatgttttac
     6721 aagagcacgg attgagttac cgtgtgcacc aacaaacaca tttttaccat ctttaagagc
     6781 aggagcaatt ttatcttccc agaaaggaag agcacgctct aaagtaactt ttaggttttc
     6841 tgcatctgga ataacagaat catctagtga agcatagcga cgatcagtat gtgctgaatg
     6901 ttcatcatct ttagccatat ctggaggcaa tacatcatat gaacgacgcc aaatatgaac
     6961 ttgctcatca ccaaattgtt cagctgcttc tgctttattt tttcctgtca atccaccgta
     7021 atgacgttcg ttcaagcgcc atgatttttc aactggtacc caaagttgat cagctgcttc
     7081 aagggcaagg ttagttgttt tgatggcacg tttaagaact gatgtaaaag caaggtcgaa
     7141 ctcaatacct gctgcttgaa ttaatttccc agcatcaata gcttgttgtg tacctttttc
     7201 tgaaagatct acgtcagccc atccagtgaa aaggttagct ttattccact cagattcacc
     7261 gtggcgtgcg aatactaatt ttaccattag aaaatttctc ctttaatttt ggctatacca
     7321 atatttactt taactattct acaaaaaata gccataaaaa tctagtctga ttactagttc
     7381 aataatcaaa aatagcttat tattaactat ctataagaaa aggcatatca cctagagtgc
     7441 tatgccttta gatatcatgt gatttattta acaaaatcta ataaggcaag gaagctttct
     7501 gcttcaagtg aagctccacc tacaagggca ccgtcaacgt ctgggcaagc catatattct
     7561 gcaacgtttt caggtttaac tgaaccaccg tattgaacgc gaactttgtc agcaactgct
     7621 tgaccgaagt ctgctgcaac aacgtcacga actgctttac acatgttttg tgcgtcgtct
     7681 tgagttgctg atttaccagt accaatagcc cagattggtt cgtaagcgat aacaagtgat
     7741 gatacttgct cttctgacaa acctgcaaga gctgctgaaa cttgagcacc tacgaattca
     7801 accgctttac cagcttcgta agtttcaaga gactcaccgc aacaaatgat tggagtcaaa
     7861 ccattagcaa agatagcttt tgcttttttg ttgatatctt ggtcagtttc atggaaataa
     7921 tcacgacgtt ctgagtgacc gataacaacg tagtctgtac ccatttcagc caatacttta
     7981 gggctgtttt caccagtgaa tgcacctgaa ttctcaaaat agctattttg agcagcgatt
     8041 ttcaattctg aacctttagc tgcttcaaga actgttgata aagtaagcgc tggagcagca
     8101 atccctgctt caacaagttc tgatgatggt aatttagaag ctactgcttc gatgaatgct
     8161 tttgcttctt ctggattttt attcattttc cagttaccag cgataaatgg tttacgtgac
     8221 attacacata cctcttcttt ttttatttac taatctatca taccataaat tatagttaat
     8281 taaaagtttc acgagtctag taaaacgctt taattataga tactaaaaaa gagtccctga
     8341 ggactcttca caatttgagc tgtctgactc taactgccgt ttgggaactt aattaagtta
     8401 aattaagctt cgatttctga aacgatacct gaaccaacag tacgtccacc ttcacggatt
     8461 gagaaagtag taccttgttc tacggcgatt gggtgaatca attcaacttc gatagtaacg
     8521 ttatcaccag gcataaccat ttctgttcct gctggaagtt cgattgaacc tgttacgtca
     8581 gttgtacgga agtagaattg tggacggtag ttgttgaaga atggagtatg acgtccacct
     8641 tcttctttag aaaggatgta aacttcacct ttaaatttag tgtgtgggtt gattgaacct
     8701 ggtttagcaa gaacttgacc acgttcgatt tcatcacgtt gaacaccacg aagaagaaca
     8761 ccaacgttgt cccctgcaag accttcgtca agttgtttac ggaacatttc aacaccagta
     8821 acaactgctt tttggatatc ttctttaata ccaacgattt caacttcgtc gttgacacga
     8881 acagtaccac ggtcgatacg tcctgaagca actgtaccac gtccagtgat tgagaataca
     8941 tcttcaactg gaagaagtaa aggtttgtca gtatcacgtt ctggttctgg aatgtactca
     9001 tcaacagtgc tcatcaattc catgatgatg tcttcgtatt tttcgtcgcc ttcaagtgct
     9061 ttaagagctg aaccttggat aactggaagg tcatcacctg ggaagtcgta ttctgaaaga
     9121 aggtcacgaa tttccatttc aaccaattca agcaattctt catcatcaac aaggtcaact
     9181 ttgttcatga atacgataag gtgtttaaca ccaacttgac gtgaaagaag gatgtgctca
     9241 cgagtttgtg gcattggtcc atcagttgaa gcaactacaa ggatagctcc gtccatttga
     9301 gcagcaccag tgatcatgtt tttaacgtag tccgcgtgtc ctggagcgtc gatgtgggca
     9361 tagtgacgtt tttcagtttc gtactcaacg tgtgcagtgt tgattgtgat tccgcgttcg
     9421 cgctcttctg gagcagcatc gatagaagcg taatcttttg gttggttaac tgaagtaggc
     9481 aagcgacgtg ccaatacagt tgtgatagct gctgttaaag tagttttacc gtggtcaacg
     9541 tgtccgattg taccaatgtt aacgtgtggt ttactacgat cgtatttttc ttttgccatt
     9601 ttaggaaaag cctccaataa aatatatttt atagatagac ggtaggcaat acagtctaac
     9661 tttacttatc tattttaaca aaatcctcta aaaaagcaag tattttatgc ttttttgctt
     9721 atttctttat ttccttaaaa ttcgggtaag attgattgag gcatttgtta tctcttatat
     9781 gtatttttta gtagttatca tataatcata ttgttaaata catcctaatt tatagtatta
     9841 gaatattaat agacacttct tttcttcaaa tatcttctct ctattaatta gagtttaaat
     9901 ttcaaaaaat acaaaaatcg agataaaaat ctcgattttt gtttggatga ttatttaaag
     9961 gcatcaaggt tgattatttt ttcatttttt tcttgtggct tatattgctc ttctgcttct
    10021 ttcattatga gttccttttt ctcgttagca tcaatattaa gcacgaatcc gatagctaca
    10081 gaaagtacca agaggctgtt ccctccttgg gataagaaag gaaatgtcac tccagttgag
    10141 gggattaacc cagaaatacc accaatgtta acaaaaactt gcatcaatag cattgctcca
    10201 ataccaagag cgatcattga gttgaaagga tctttagcct ttatacctac gtgcataata
    10261 cgtaaaatta agaagaaaac caaagctaaa ataaaccctg caccaataac tcctaattcc
    10321 tcaataacaa ttgaaaagac aaagtctgtt gttgcttctg gtaagtaccc taatttttca
    10381 atagaattcc caagtcctcg accaaaccag ccaccgttgc tcattgcata atacgaatta
    10441 gccaactggt gtcctgaatc tgttaaatct ttaaatgggt caaaaaaagc attaaatcgc
    10501 ttagctacat aaccaaagac agggacttta gccattgtct caactccaac aactgcaata
    10561 gttccgataa ataaagttga ccccacgacg attagaccaa gtaaagctga aaaccatcga
    10621 taaccaatac cactaataca gtacataata atgaccgtta gaacaattat cgaaccattt
    10681 cctaaatcag gttgtgcaat taccaaacca atcataaata aagaataaaa tcgccaatct
    10741 tttaggtcgc ttagtgatct cggaagccat ctcccttttg tcaaagcttg ataatcatat
    10801 atttctatct ttttttgcct tcgagcaaaa gtaaacgcta aataccatac aatgataact
    10861 tttaaatatt cagcaggttg aaaactaata ggaccaataa caatccaacc atgtgctcca
    10921 ttaacttctt gtgtaaaaaa gcgcgctatt agtagtaaaa atacttctac caatactgcc
    10981 atagtcaaca ctttagaatt tttcaaaaaa tttagtttca gtttatagat aaagataatt
    11041 gcaaccaaac tgactgccca aaagacaccc tggttgatta ctgaacgaaa agggttagct
    11101 ccaagctgaa ttagagttgc actagttgtc gaataaatta caattaagcc tagaatagac
    11161 aatatcaaat agggaatgag gatggaataa ttcagaagat g
//
LOCUS       DATRSD010000067        11183 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRSD010000067 DATRSD010000000
VERSION     DATRSD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.068"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            252..2957
                     /gene="pilA"
                     /locus_tag="VBD85_001524"
     CDS             252..2957
                     /gene="pilA"
                     /locus_tag="VBD85_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus adhesin PilA"
                     /protein_id="HEO7134072.1"
                     /translation="MRKYQKFSKILTLSLFCLSQIPLNTNVLGESTVPENGAKGKLVV
                     KKTDDQNKPLSKATFVLKTTAHPESKIEKVTAELTGEATFDNLIPGDYTLSEETAPEG
                     YKKTNQTWQVKVESNGKTTIQNSGDKNSTIGQNQEELDKQYPPTGIYEDTKESYKLEH
                     VKGSVPNGKSEAKAVNPYSSEGEHIREIPEGTLSKRISEVGDLAHNKYKIELTVSGKT
                     IVKPVDKQKPLDVVFVLDNSNSMNNDGPNFQRHNKAKKAAEALGTAVKDILGANSDNR
                     VALVTYGSDIFDGRSVDVVKGFKEDDKYYGLQTKFTIQTENYSHKQLTNNAEEIIKRI
                     PTEAPKAKWGSTTNGLTPEQQKEYYLSKVGETFTMKAFMEADDILSQVNRNSQKIIVH
                     VTDGVPTRSYAINNFKLGGSYESQFEQMKKNGYLNKSNFLLTDKPEDIKGNGESYFLF
                     PLDSYQTQIISGNLQKLHYLDLNLNYPKGTIYRNGPVKEHGTPTKLYINSLKQKNYDI
                     FNFGIDISGFRQVYNEEYKKNQDGTFQKLKEEAFKLSDGEITELMRSFSSKPEYYTPI
                     VTSADTSNNEILSKIQQQFETILTKENSIVNGTIEDPMGDKINLQLGNGQTLQPSDYT
                     LQGNDGSVMKDGIATGGPNNDGGILKGVKLEYIGNKLYVRGLNLGEGQKVTLTYDVKL
                     DDSFISNKFYDTNGRTTLNPKSEDPNTLRDFPIPKIRDVREYPTITIKNEKKLGEIEF
                     IKVDKDNNKLLLKGATFELQEFNEDYKLYLPIKNNNSKVVTGENGKISYKDLKDGKYQ
                     LIEAVSPEDYQKITNKPILTFEVVKGSIKNIIAVNKQISEYHEEGDKHLITNTHIPPK
                     GIIPMTGGKGILSFILIGGAMMSIAGGIYIWKRYKKSSDMSIKKD"
     gene            3087..5114
                     /locus_tag="VBD85_001525"
     CDS             3087..5114
                     /locus_tag="VBD85_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO7134073.1"
                     /translation="MKKINKYFAVFSALLLTVTSLFSVAPVFAEEAKTTDTVTLHKIV
                     MPRTAFDGFTAGTKGKDNTDYVGKQIEDLKTYFGSGEAKEIAGAYFAFKNEAGTKYIT
                     ENGEEVDTLDTTDAKGGAVLKGLTTDNGFKFNTSKLTGTYQIVELKEKSTYNNDGSIL
                     ADSKAVPVKITLPLVNDNGVVKDAHVYPKNTETKPQVDKNFADKELDYANNKKDKGTV
                     SASVGDVKKYHVGTKILKGSDYKKLIWTDSMTKGLTFNNDIAVTLDGATLDATNYKLV
                     ADDQGFRLVLTDKGLEAVAKAAKTKDVEIKITYSATLNGSAVVEVLETNDVKLDYGNN
                     PTIENEPKEGIPVDKKITVNKTWAVDGNEVNKADETVDAVFTLQVKDGDKWVNVDSAK
                     ATAATSFKHTFENLDNAKTYRVIERVSGYAPEYVSFVNGVVTIKNNKDSNEPTPINPS
                     EPKVVTYGRKFVKTNKDGKERLAGATFLVKKDGKYLARKSGVATDAEKAAVDSTKSAL
                     DAAVKAYNDLTKEKQEGQDGKSALATVSEKQKAYIDAFVKANYSYEWVEDKNAKNVVK
                     LISNDKGQFEITGLTEGQYSLEETQAPTGYAKLSGDVSFNVNATSYSKGSAQDIEYTQ
                     GSKTKDAQQVINKKVTIPQTGGIGTIFFTIIGLSIMLGAVVIMKRRQSEEV"
     gene            5226..6104
                     /locus_tag="VBD85_001526"
     CDS             5226..6104
                     /locus_tag="VBD85_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO7134074.1"
                     /translation="MKTKKIIKKTKKKKSNLPFIILFLIGLSILLYPVVSRFYYTIES
                     NNQTQDFERAAKKLSQKEINRRMALAQAYNDSLNNVHLEDPYEKKRIQKGIAEYARML
                     EVSEKIGIISVPKIGQKLPIFAGSSQEVLSKGAGHLEGTSLPIGGNSTHTVITAHSGI
                     PDKELFSNLKKLKKGDKFYIQNIKETIAYQVDQIKVVTPDNFSDLLVVPGHDYATLLT
                     CTPIMVNTHRLLVRGHRIPYKGPIDEKLIKDGHLNTIYRYLFYISLVIIAWLLWLIKR
                     QRQKNRLSSVRKGIES"
     gene            6104..6988
                     /gene="srtC4"
                     /locus_tag="VBD85_001527"
     CDS             6104..6988
                     /gene="srtC4"
                     /locus_tag="VBD85_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus assembly sortase SrtC4"
                     /protein_id="HEO7134075.1"
                     /translation="MRGKFQKNLKKSVVLNRWMNIGLILLFLVGLLITSYPFISNWYY
                     NIKANNQVTNFDNQTQKLNAKEINRRFELAKAYNRTLDPSRLSDPYTEKEKKGIAEYA
                     HMLEITEMIGYIDIPSIKQKLPIYAGTTSSVLEKGAGHLEGTSLPIGGKSSHTVITAH
                     RGLPKAKLFTDLDKLKKGKIFYIHNIKEVLAYKVDQISVVKPDNFSKLLVVKGKDYAT
                     LLTCTPYSINSHRLLVRGHRIKYVPPVKEKNYLMKEFQTHYKLYFLLSILVILILVAL
                     LLYLKRKFKERKRKGNQK"
     gene            6985..7911
                     /gene="pilC"
                     /locus_tag="VBD85_001528"
     CDS             6985..7911
                     /gene="pilC"
                     /locus_tag="VBD85_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus subunit PilC"
                     /protein_id="HEO7134076.1"
                     /translation="MKKIRKSLGLLLCCFLGLVQLAFFSVASVNADTPNQLTITQIGL
                     QPNTTEEGISYRLWTVTDNLKVDLLSQMTDSELNQKYKSILTSPTDTNGQTKIALPNG
                     SYFGRAYKADQSVSTIVPFYIELPDDKLSNQLQINPKRKVETGRLKLIKYTKEGKIKK
                     RLSGVIFVLYDNQNQPVRFKNGRFTTDQDGITSLVTDDKGEIEVEGLLPGKYIFREAK
                     ALTGYRISMKDAVVAVVANKTQEVEVENEKETPPPTNPKPSQPLFPQSFLPKTGMIIG
                     GGLTILGCIILGILFIFLRKTKNSKSERNDTV"
     regulatory      7998..8143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8213..8797
                     /locus_tag="VBD85_001529"
     CDS             8213..8797
                     /locus_tag="VBD85_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7134077.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     gene            8778..9419
                     /locus_tag="VBD85_001530"
     CDS             8778..9419
                     /locus_tag="VBD85_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7134078.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSIIILLQYYMPKSIWKLLCQ
                     GILGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLAKDWLKKVK"
     gene            9512..10447
                     /locus_tag="VBD85_001531"
     CDS             9512..10447
                     /locus_tag="VBD85_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO7134079.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRIRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMTNTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            10440..11006
                     /locus_tag="VBD85_001532"
     CDS             10440..11006
                     /locus_tag="VBD85_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7134080.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGHDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSEKDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
BASE COUNT         4045 a         1700 c         2059 g         3379 t
ORIGIN      
        1 ttaagcaata ttttaaaaca taaaaaaaga aaaatcaact acttaagctc attgaaatat
       61 ttctaagata ataaaaaata agattatcaa ataaaaagaa aaattattca aaaattggga
      121 aaaaacatta aaattccata ccttataata agaaattatt gatataataa tccatatttt
      181 gtatattcta ggatattctg tatctgatct tagatttaga aacgacattt cggcacaata
      241 ggagttgtaa aatgagaaaa taccaaaaat tttctaaaat attgacgtta agtctttttt
      301 gtttgtcgca aataccgctt aataccaatg ttttagggga aagtaccgta ccggaaaatg
      361 gtgctaaagg aaagttagtt gttaaaaaga cagatgacca gaacaaacca ctttcaaaag
      421 ctacctttgt tttaaaaact actgctcatc cagaaagtaa aatagaaaaa gtaactgctg
      481 agctaacagg tgaagctact tttgataatc tcatacctgg agattatact ttatcagaag
      541 aaacagcgcc cgaaggttat aaaaagacta accagacttg gcaagttaag gttgagagta
      601 atggaaaaac tacgatacaa aatagtggtg ataaaaattc cacaattgga caaaatcagg
      661 aagaactaga taagcagtat ccccccacag gaatttatga agatacaaag gaatcttata
      721 aacttgagca tgttaaaggt tcagttccaa atggaaagtc agaggcaaaa gcagttaacc
      781 catattcaag tgaaggtgag catataagag aaattccaga gggaacatta tctaaacgta
      841 tttcagaagt aggtgattta gctcataata aatataaaat tgagttaact gtcagtggaa
      901 aaaccatagt aaaaccagtg gacaaacaaa agccgttaga tgttgtcttc gtactcgata
      961 attctaactc aatgaataac gatggcccaa attttcaaag gcataataaa gccaagaaag
     1021 ctgccgaagc tcttgggacc gcagtaaaag atattttagg agcaaacagt gataataggg
     1081 ttgcattagt tacctatggt tcagatattt ttgatggtag gagtgtagat gtcgtaaaag
     1141 gatttaaaga agatgataaa tattatggcc ttcaaactaa gttcacaatt cagacagaga
     1201 attatagtca taaacaatta acaaataatg ctgaagagat tataaaaagg attccgacag
     1261 aagctcctaa agctaagtgg ggatctacta ccaatggatt aactccagag caacaaaagg
     1321 agtactatct tagtaaagta ggagaaacat ttactatgaa agccttcatg gaggcagatg
     1381 atattttgag tcaagtaaat cgaaatagtc aaaaaattat tgttcatgta actgatggtg
     1441 ttcctacgag atcatatgct attaataatt ttaaactggg tggatcatat gaaagccaat
     1501 ttgaacaaat gaaaaaaaat ggatatctaa ataaaagtaa ttttctactt actgataagc
     1561 ccgaggatat aaaaggaaat ggggagagtt actttttgtt tcccttagat agttatcaaa
     1621 cacagataat ctctggaaac ttacaaaaac ttcattattt agatttaaat cttaattacc
     1681 ctaaaggtac aatttatcga aatggaccag tgaaagaaca tggaacacca accaaacttt
     1741 atataaatag tttaaaacag aaaaattatg acatttttaa ttttggtatc gatatatctg
     1801 gttttagaca agtttataat gaggagtata agaaaaatca agatggtact tttcaaaaat
     1861 tgaaagagga agcttttaaa ctttcagatg gagaaatcac agaactaatg aggtcgttct
     1921 cttccaaacc tgagtactac acccctatcg taacttcagc cgatacatct aacaatgaaa
     1981 ttttatctaa aattcagcaa caatttgaaa cgattttaac aaaagaaaac tcaattgtta
     2041 atggaactat cgaagatcct atgggtgata aaatcaattt acagcttggt aatggacaaa
     2101 cattacagcc aagtgattat actttacagg gaaatgatgg aagtgtaatg aaggatggta
     2161 ttgcaactgg tgggcctaat aatgatggtg gaatacttaa gggggttaaa ttagaataca
     2221 tcggaaataa actctatgtt agaggtttga atttaggaga aggtcaaaaa gtaacactca
     2281 catatgatgt gaaactagat gacagtttta taagtaacaa attctatgac actaatggta
     2341 gaacaacatt gaatcctaag tcagaggatc ctaatacact tagagatttt ccaatcccta
     2401 aaattcgtga tgtgagagaa tatcctacaa taacgattaa aaacgagaag aagttaggtg
     2461 aaattgaatt tataaaagtt gataaagata ataataagtt gcttctcaaa ggagctacgt
     2521 ttgaacttca agaatttaat gaagattata aactttattt accaataaaa aataataatt
     2581 caaaagtagt gacgggagaa aacggcaaaa tttcttacaa agatttgaaa gatggcaaat
     2641 atcagttaat agaagcagtt tcgccggagg attatcaaaa aattactaat aaaccaattt
     2701 taacttttga agtggttaaa ggatcgataa aaaatataat agctgttaat aaacagattt
     2761 ctgaatatca tgaggaaggt gacaagcatt taattaccaa cacgcatatt ccaccaaaag
     2821 gaattattcc tatgacaggt gggaaaggaa ttctatcttt cattttaata ggtggagcta
     2881 tgatgtctat tgcaggtgga atttatattt ggaaaaggta taagaaatct agtgatatgt
     2941 ccatcaaaaa agattaaaaa ttatgcgctt tagtattctt aattaattaa ctaaaattcc
     3001 atgacttgaa gggagtggtg ctgcggtgta ttaaaatccg catattattt atcatgttga
     3061 ttaactaaaa taagaaggag atagaaatga aaaaaatcaa caaatatttt gcagtcttct
     3121 cggcattgct actgaccgta acatcattgt tctcagttgc accagtgttt gcggaagaag
     3181 caaaaactac tgacacagtg accttgcaca agattgtcat gcctcgaact gcatttgacg
     3241 gttttactgc tggtacaaag ggtaaggata atactgacta cgttggtaaa caaatcgaag
     3301 accttaaaac ttactttggc tcaggcgaag cgaaagaaat cgcaggtgct tactttgctt
     3361 tcaaaaatga agctggtact aaatacatca ctgaaaatgg tgaagaagtt gatactttgg
     3421 atacaacaga tgccaaaggt ggtgctgttc ttaaaggttt aacaacagac aatggtttca
     3481 aatttaacac ttctaaatta acaggaactt accaaatcgt tgaattgaaa gaaaaatcta
     3541 catacaacaa cgatggttct atcttggctg attcaaaagc agttccagtt aaaatcactc
     3601 ttccattggt aaacgacaat ggtgttgtta aagacgctca cgtttatcca aagaacactg
     3661 aaacaaaacc acaagtagat aagaacttcg cagataaaga acttgattat gcgaacaaca
     3721 aaaaagacaa agggactgtc tcagcatctg ttggtgatgt taaaaaatac catgttggaa
     3781 caaaaatcct taaaggttca gactataaga aattaatctg gaccgatagc atgaccaaag
     3841 gtttgacttt caacaacgat attgctgtaa cattggatgg tgcaactctt gatgctacaa
     3901 attacaaact tgtagcagat gaccaaggtt tccgccttgt cttgactgac aaaggtcttg
     3961 aagcagtggc aaaagccgca aaaacaaaag atgttgaaat caagatcact tactcagcta
     4021 ctttgaacgg ttctgctgtc gttgaagttc tagaaaccaa tgatgttaaa ttggactacg
     4081 gcaacaaccc aacaattgaa aatgaaccaa aagaaggtat tccagttgat aagaaaatca
     4141 ctgttaacaa aacatgggca gtagatggca atgaagtgaa taaagcagat gaaacagttg
     4201 atgctgtctt caccttgcaa gttaaagatg gtgacaaatg ggtgaatgtt gattcagcta
     4261 aagcaacagc tgcaactagc ttcaaacaca cttttgaaaa cttggataat gctaaaactt
     4321 accgcgttat cgaacgtgtt agcggctacg ctccagaata cgtctcattt gtaaatggcg
     4381 ttgtaaccat caagaacaac aaagactcaa atgagccaac tccaatcaac ccatcagaac
     4441 caaaagtggt gacttatgga cgtaaatttg tgaaaacaaa taaagatgga aaagaacgct
     4501 tggcaggagc taccttcctt gttaagaaag atggcaagta cttggcacgt aaatcaggtg
     4561 ttgcaacaga tgcagaaaaa gctgctgtag attcaactaa atcagcattg gatgctgctg
     4621 ttaaagctta caatgatttg actaaagaaa aacaagaagg tcaagatggt aaatcagcat
     4681 tggctaccgt tagtgaaaaa caaaaagctt acattgatgc ctttgttaaa gctaactact
     4741 catacgaatg ggttgaagat aaaaatgcta agaatgttgt taaattgatt tctaacgata
     4801 aaggtcaatt tgaaattact ggcttgactg aaggtcaata ctcattggaa gaaacacaag
     4861 caccaactgg ttatgctaaa ttatcaggtg atgtttcgtt taatgttaat gctacttcat
     4921 acagtaaagg ttctgctcaa gatattgagt atacccaagg ttctaaaact aaagatgcac
     4981 aacaagttat caataagaag gttactattc cacaaacagg tggtattggt acaatttttt
     5041 tcacaattat tggattaagt attatgcttg gagcggtagt tatcatgaaa agacgtcaat
     5101 cagaggaagt ttaagattag cttttgatga tgtgtaagca cagttaaagc tgtgcttatg
     5161 atctaagggt atttcagtag aagtactctt agatcataag caagagccat tatttaggag
     5221 atgacgtgaa gactaaaaaa attatcaaaa aaacaaaaaa aaagaaatca aatctgcctt
     5281 ttatcattct ttttctaata ggtctatcta tcttattgta tccagtggta tcacgatttt
     5341 actatacgat agaatctaat aaccaaacac aagattttga gagagctgct aaaaaactta
     5401 gtcagaaaga aatcaatcgg cgtatggctc tagcacaagc atataatgat tctttaaata
     5461 atgtccatct tgaagatcct tatgagaaaa aacgaattca aaaggggata gcagagtacg
     5521 cccgtatgtt agaggtaagt gaaaaaatcg gcataatttc agttcctaag ataggtcaaa
     5581 aactccctat atttgcaggt tcaagtcaag aagttctatc taaaggagca gggcatttag
     5641 aaggtacctc tcttccaatt ggaggcaata gcacccatac cgttataaca gcgcactcag
     5701 gaattccaga taaagagctc ttttctaacc ttaaaaagtt aaaaaaagga gataagtttt
     5761 atattcaaaa cataaaagaa acgatagcat atcaagtaga tcagataaaa gtcgttacac
     5821 ccgataactt ttcagatttg ttggttgttc ctggacatga ttatgcaact ttattaactt
     5881 gcaccccgat tatggtcaat acacacagac ttttagtaag gggtcatcgt atcccttata
     5941 aaggtcctat tgatgaaaaa ttaataaaag acggtcattt aaacacgatt tatagatatc
     6001 ttttctatat atctttagtt attattgctt ggttactttg gttaataaaa cgtcaacgtc
     6061 aaaaaaatcg tttatcaagt gttagaaaag gaattgaatc ataatgcgag gaaaatttca
     6121 gaaaaacctt aagaaatcgg tcgttttaaa tcgatggatg aatataggtt tgatattatt
     6181 attcttagta ggtcttttga taacctcata tccttttatt tcaaattggt actataatat
     6241 taaagctaat aatcaagtaa ctaactttga taatcaaacc caaaaattaa atgctaaaga
     6301 gattaataga cgatttgagt tagcaaaagc ttataataga acactggacc caagccgcct
     6361 atcagatccc tatactgaaa aagaaaaaaa aggtattgct gaatacgccc atatgcttga
     6421 gattactgaa atgattggat atattgatat accgtctatc aagcaaaaat tacctatcta
     6481 tgcggggact accagtagtg ttcttgaaaa aggagcagga caccttgaag gaacttcttt
     6541 gccaattggt ggaaaaagtt cacatactgt tatcacagct catcgcggct tacctaaagc
     6601 taagttattt acagatttag ataaacttaa aaaaggaaaa attttttata ttcataatat
     6661 caaagaagtt ttagcctata aggttgatca aataagtgtt gtaaagccag ataatttttc
     6721 taaattattg gttgttaaag gtaaggatta tgcgactttg ctaacatgta caccttattc
     6781 gattaattca catcgtttac tagttagagg gcatcgaatc aagtatgtac ctcctgttaa
     6841 agaaaagaac tatttaatga aagaatttca aacacactat aaactttatt tcctcttatc
     6901 aatcctagtt attcttatat tagtcgcttt actattatat ttaaaacgaa aatttaaaga
     6961 gagaaagaga aagggaaatc aaaaatgaaa aagattagaa aaagtttagg acttctacta
     7021 tgttgctttt taggattggt acaattagcg tttttttcgg tagccagtgt aaatgctgat
     7081 acccctaatc aactaacaat cacacagata ggacttcagc caaatactac agaggagggg
     7141 atttcttatc gtttatggac tgtgactgac aacttaaaag ttgatttatt gagccaaatg
     7201 acagatagcg aattgaacca gaagtataag agtatcttga cttctcctac tgatactaat
     7261 ggtcagacaa agatagcact cccaaatggt tcgtactttg gtcgtgctta taaagctgat
     7321 caaagcgttt caacaatagt acctttttat attgaattac cagatgataa gttatcaaat
     7381 caattacaga taaatcctaa gcgaaaagtt gaaacaggcc gattaaaact tattaaatat
     7441 acaaaagaag gaaagataaa gaaaaggcta tcaggagtaa tatttgtatt atacgataac
     7501 cagaatcagc cagttcgctt taaaaatgga cgatttacga ccgatcaaga tgggattact
     7561 tcattagtaa ctgatgataa gggagaaatt gaggttgaag gtttattacc tggtaagtat
     7621 atttttcgag aagcaaaagc actaactggt taccgtatat ctatgaagga tgctgtagtt
     7681 gctgtagttg ctaataaaac acaggaagta gaggtagaaa acgaaaaaga aactcctcca
     7741 ccaacaaatc ctaaaccatc acaaccgctt tttccacaat catttcttcc taaaacagga
     7801 atgattattg gtggaggact gacaattctt ggttgtatta ttttgggaat tttgtttatc
     7861 tttttaagaa aaactaaaaa tagcaaatct gaaagaaacg atacagtata gtaatttggt
     7921 attaatatga tttttaatca ctactactaa ttagtaatta aaaaacttgc aattgcaagt
     7981 tttttttgtt atgctatagg tgtcttcagg gcagggtgta attcccgacc ggcggtaatc
     8041 gcttccaact tatttgtcag ttgtgaagca tcagtccgcg agcgaaagct gatgtggtga
     8101 gattccacaa ccgacagtaa agtctggatg ggagaagaca aaggtttttt tcgcgtacct
     8161 acgcttacct ttggagcttc tttcaattta tttaaattgg aggaattttt ttatgacaaa
     8221 tacacgtaaa atggtgacaa tcgccattct ttctgcctta tcttttgtat tgatgatggt
     8281 ttcatttcca ttaataccag gagcagaatt tttaaaggtt gatttttcaa tccttccgat
     8341 gttagttgct tttattttat ttgatttgaa atcaagttat ggtgtactct tattaagatc
     8401 acttttaaaa gttatacttg ctaatcgtgg tccagaaacc tttatcggtt taccgatgaa
     8461 tatggttgct ctagctctat ttttagcaag tttcgctatt ttctggaaaa atagagagag
     8521 tgcaaaagat tttattaaag ctagtttatt tggtacagtt agcttgactg tatctatggt
     8581 agctctcaac tatgtctttg cgattccttt gtatgctata tttgctaact ttgatatcag
     8641 aacatttatt ggtgtaggca attacctttt aacgatggtt attccattta atatcgttga
     8701 gggaatctta atttcgattg ttttctatct aacttacgtg gcttgtttgc caatattaga
     8761 aagatataaa aagactaatg ttatctcgtc aaaatagtaa acttattcaa gcatttattg
     8821 ctattatctt atttttcagt ttaggattgg tcatcaaata ttggcccgac actgtaatcg
     8881 catttgacca aactattcaa gaaagtgtta gaggacagtt gccaaatctt tctacaagat
     8941 ttttcaagtt aatcactgtg gttggtaata ctgtaagcca gatagctatt gcgattatga
     9001 gcgtcacttt ctgttacctt aagaagtggt atcctcaagc tagatttatc gcagtaaatg
     9061 ctattatctc tggtatatgt atactttctt taaaacttat ttttcaaaga gtgagaccaa
     9121 ctttaaccca tttagtattt gcaggtggct attcgtttcc cagtgggcac tcgatgggaa
     9181 catttatgat atttgggagt attattattt tattgcagta ctatatgcct aagagtatat
     9241 ggaaactgtt gtgccaaggt attttgggtt tgctcatttt tttaattggg ttatctcgta
     9301 tttacctagg ggttcatttt ccgacagatg ttttagctgg ttttatactt gcttatggta
     9361 ttttgaatct aactgcgtat atctttttag ccaaggattg gttaaagaaa gtgaagtaaa
     9421 taaaagattg ggactaactg acgttccaat cttttttagt tttattgtag tagtgaggtt
     9481 attttagtta aaattgtgat acaattatcc tatgaaaaaa cgttatcgtg cgattaatga
     9541 ttattatcga gaactctttg gagaaaaaat atttaaatta cctatagatg ctggttttga
     9601 ctgtcctaac agagacggaa cagttgctag aggaggatgc actttttgca cagtttctgg
     9661 ttcaggggat gctatagttg ctccagaagc tcctattcgt gaacaattct ataaagaaat
     9721 tgatttcatg cacagaaagt ggcccgaggt taataaatac ttagtctatt ttcaaaattt
     9781 taccaatacc catgctaagt tagagataat taaagagcgt tacgaacagg ctattaatga
     9841 gccaggagtt atcgggatca acatcggaac aaggccggat tgcttacctg atgaaactat
     9901 atactatctc gctgaacttt ctgaaagaat gcatgtgact ttagaattag gccttcagac
     9961 aacttatgaa gcaacttctg cattaataaa tagagcacat tcatatgacc tttacaaaaa
    10021 aactgttaag agaatacgag aattagctcc taaagtagaa atcgtttctc acctaataaa
    10081 tggtctccca ggggagacac atgatatgat ggtggaaaat gttcgtcgat gtgttacgga
    10141 taatgatatt cagggtataa aattacacct tctgcatctt atgactaata caagaatgca
    10201 gagagattac catgaaggtc gtttacgttt gttaagtcaa gaagactata tttcgattat
    10261 ttgtgaccaa ctggagatta ttccaaaaca tattgttatt caccgaatta ctggtgatgc
    10321 gccacgtcat atgttaattg gaccgatgtg gagccttaat aaatgggaag tgcttaatgc
    10381 cattgacaaa gaaatggaga aaagacagtc atatcaagga tgtaaagttg aggtaaaaca
    10441 tgattaaaag accaattcat ctttctcatg attttttagc agaagttatt gataaagaag
    10501 caataacgct tgatgcaact atgggaaatg ggcatgatac tgttttttta gcgaaaagtt
    10561 ctaaaaaagt ttatgcattt gatattcaag aagaagcaat agcaaaaacg aaagcaaagc
    10621 tcacggagca agggatttct aacgctgaac ttatcttgga tgggcatgaa aatttggaac
    10681 agtatgttca tacaccttta agagcggcta tatttaatct gggatactta ccctcagcgg
    10741 ataaaacagt tattacaaaa cctcacacaa ctatcaaggc aatcaaaaac gttttagaca
    10801 ttttagaagt aggtggacgt ctatcactta tggtttatta tggtcatgat ggtggaaaaa
    10861 gtgagaaaga tgcggtgata gcattcgtgg aacaattacc acagaataat ttcgctacaa
    10921 tgctttatca accactcaat caagttaata cgcccccctt tttaattatg gtagaaaagt
    10981 tacaatcata tgaaaatgag gtgtaactat ctttaggcaa ttagctaaaa tgataattgc
    11041 tataaaatgc cataaacaat attatcgact atgcgctcag tgctattata tgatataatg
    11101 atagcattat ctaatagaag aacagttggt agagttaatc gctatcagct aaattcaaat
    11161 aatttttatt taggagtaat ttt
//
LOCUS       DATRSD010000068        11122 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRSD010000068 DATRSD010000000
VERSION     DATRSD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.069"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(285..1787)
                     /gene="pyk"
                     /locus_tag="VBD85_001533"
     CDS             complement(285..1787)
                     /gene="pyk"
                     /locus_tag="VBD85_001533"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO7134081.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGFVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            complement(1836..2858)
                     /gene="pfkA"
                     /locus_tag="VBD85_001534"
     CDS             complement(1836..2858)
                     /gene="pfkA"
                     /locus_tag="VBD85_001534"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO7134082.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHKADLRLAALNRDL
                     ANQSSK"
     gene            complement(2939..6043)
                     /locus_tag="VBD85_001535"
     CDS             complement(2939..6043)
                     /locus_tag="VBD85_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7134083.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDVLDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKERFPEALANLESLVEN
                     VAYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEDNGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLETIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKGVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKVIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            6188..6556
                     /locus_tag="VBD85_001536"
     CDS             6188..6556
                     /locus_tag="VBD85_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7134084.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            6557..7255
                     /locus_tag="VBD85_001537"
     CDS             6557..7255
                     /locus_tag="VBD85_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134085.1"
                     /translation="MTQLLQLHHVTKKYHKHTAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFDSKVAYQLFEDLNLNPRERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            7267..8049
                     /locus_tag="VBD85_001538"
     CDS             7267..8049
                     /locus_tag="VBD85_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO7134086.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(8086..8697)
                     /locus_tag="VBD85_001539"
     CDS             complement(8086..8697)
                     /locus_tag="VBD85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO7134087.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            8790..9139
                     /gene="ssrA"
                     /locus_tag="VBD85_001540"
     tmRNA           8790..9139
                     /gene="ssrA"
                     /locus_tag="VBD85_001540"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9408..10046
                     /locus_tag="VBD85_001541"
     CDS             9408..10046
                     /locus_tag="VBD85_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO7134088.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGLNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIGAGSVITKDIPDYGVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKKEFFNTLKSISENKNHKL"
     gene            complement(10167..>11122)
                     /locus_tag="VBD85_001542"
     CDS             complement(10167..>11122)
                     /locus_tag="VBD85_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7134089.1"
                     /translation="VCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSY
                     YRAEISLEFEKQFAAMVFTIPNINYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSA
                     IKYLNTILPYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLP
                     TTEYKTTKQKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILK
                     TGTGLSLKSICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKM
                     MKAFHSFRELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
BASE COUNT         3647 a         2111 c         1759 g         3605 t
ORIGIN      
        1 aggggtagcg aattttccaa gattgacgac tatccaattc aactgcgcaa tcatcaatag
       61 aaataataaa atcaatcatc gtagtcacct catctatagt gtactacttt gattgatttt
      121 gtcattatga atctagtttt ctgaaaacaa aagtttcttc atgcgtttgg cgtgttatta
      181 atatcatatc ctattctagt cacaataatc gaataataaa aagcaaaaca tataaaaaac
      241 ccattattta aggtattctt aaataatagg tttgaattca aaaattattt aacagtacga
      301 acacgcattg tgttagttcc acctgtacct acaggaacac ctgcaacgat aacgatatta
      361 tcgcctgatt caacaaatcc tgcttcaagt gctacacgtt ctgcaacctc aaacatatca
      421 tctgtagatg ctggtttgtc tgcaaggaca gggataacac cccagttaat catcaatgaa
      481 cgttgtactt tttcatcaaa tgtaacagcc aaaatgtctg catctggacg gaatttagaa
      541 atggcacgag ctgtattacc tgtttcagta attgttacaa caagtttgat atccattgag
      601 tgtgttgcat ctttaaccgc agatgcaata acatcagttt tgttattacg tgggaatgca
      661 gatgagtcta agcgaccata ctcattgagt aatgtttgag catttttatc aatagtagcc
      721 attgtacgaa ctgactcaac tgggtattta ccattagctg actcacctga aagcattgta
      781 gcatcagtac catcaataac agcattgaag acatcagata cttctgaacg agtcgcacgt
      841 ggtttatcag tcattgtttc aagcatattt gttgctgtaa taactgcttt accagctgca
      901 ttaactttag taatgatcat tttttggtaa actggaacca tttcaaatgg aacttcgata
      961 cccatatcac cacgagcaat cataatacca tctgctgctt cgataatctc atcaatatta
     1021 tcgatacctt gttgattttc aattttagca aacaacttaa cgtgtccatt gccagtttct
     1081 tcacaaatag cacgaacttc attaacatct ttagcagtac gtacaaatga gatagcaata
     1141 aagttaagtc cttgctcaag tccaaaacgg atatcagcat tatcgcgttc tgcaagtgct
     1201 gggaaaggaa ttttagtata agggatgttt acaccttttt gtttaccaat aaggccatca
     1261 ttctcaacaa ctacttcaaa ttcacgagtg tctttatctt ttgcaaacac agtaagacct
     1321 agtttaccat catcaacaag gatttgctta ccaacttcaa cgtcatcaaa gatgtcaagt
     1381 ccaccagcaa cattcaatgc aatcacttct ggagttgatt tgataccttg cttagtagca
     1441 acacgtaatt ttgtacctgt tgtatatgaa tggaaatctg caccatcttc aaaaagttct
     1501 gtacgaattt caggtccttt agtatcaagg aggaagccaa ctttttgtcc tgcaatctct
     1561 tctgctttac gaacagtagc catacgagct ccttgctcag catgatctcc atgtgagaag
     1621 ttgaaacgga aaacgttagc accttcttta atcaattgag caattttttc tgctgaagct
     1681 tctacgtcaa ggctttcacc ccagtatcca gactcaccaa acttcttacc accacggaat
     1741 tcaaccgcag gaccaagtgt tgcaacgatt tttacgcgtt tattcataat aatttatgag
     1801 ctccttttta tttaaagtgt caaattgaca aattcttatt tactactttg gttggcaagg
     1861 tcacgattaa gtgctgccaa gcgaaggtcc gctttatgcg gattattaac aacgattttt
     1921 ccttcatcag tcaagctgaa caaagcacct tcttctgcta aacctaaaat tggactttca
     1981 accatttctt cgttgtggac accaacggct aaaccaccac gaccttcttt caacaattga
     2041 acagcgtacg ctcccatacg agatgctaag acacgatcac gagccgtcgg actaccacca
     2101 cggagcagat gtcctaaatt cgtcacacga agatcgctat cgtctcctgc tgctttcatt
     2161 gtttttgcaa actcatcacc actcataaca ccttctgcaa ggacgatgat ttggtgatgt
     2221 ttaccagctg catagccagc tctaacattt gagacaactt catcaatatt gaactcttct
     2281 tcaggaacaa taatttgatc tgcacctgca gcgatacctg accaaagagc gatatctcct
     2341 gcatttcttc ccataacctc aacaacaaaa gtacggttat gacttgctga tgtatcacga
     2401 agacggtcaa gattctcaac tgctgtcgca actgctgtgt caaaaccaat agtatagtca
     2461 gtgccaacga tatcgttatc aattgtaccc ggcaaaccaa cagctgggaa accgtgctca
     2521 gttagacgca tagcaccatg ataagaacca tcaccaccga taactactac accttcaata
     2581 ccgtgttttt taagctgttc aatcccttta agctgacctt caagttcagc aaattcagga
     2641 taacgtgctg aacgtaaaaa cgttcctcca cggttgatag tatccccaac agaattagca
     2701 tccaaaggga aaatatcccc tgtcaccata ccatagtaac cttggttgat gccgtaaact
     2761 tccatacctt cagaaattgc tttacgaaca actgcacgga tagcagcgtt cataccaggg
     2821 gcgtcaccac cactagttaa aacagcaata cgtttcattt aattggtact ccttttatta
     2881 atttaacgat ttaattatag catattaatt tgtaaatttc tgcactttat atgcaatttt
     2941 attgataaac cgttttcaca acaaattcag ataaatcaag ggctaagcca ctatcttttc
     3001 taaccaaatg tactttgctt tggatagttt ctttagttgt ttcataatgc aaatagacag
     3061 gaatatgccc tttgtatttg gacaatattt ccgatatttt tttgtcgtta tcatgatttt
     3121 ttagaagtat ccaaaaacgt tcctccgttg cttcttgaac accgtttaaa accatctgta
     3181 atcgctcatc tcgcttctga atttttccat gcaagtaata atatttccct tcttgtaaat
     3241 catctcttac aatattgaaa gtttcaggaa aaacagtaat atccattttt gaattaatat
     3301 ccataacagt caagaaagcc atttgttgcc cagaactttt tgtcctaata actttgacct
     3361 gctttagctg aactaaaata gtagcttcgc tattttcttg aacttgacta attggtataa
     3421 acggacggct agctttttgt gaaatcccca ctaaagggtg cgggctgatt cctactccta
     3481 agatatcttg ttccatttca tatttctcag aattagagaa atcttctact tctagccaat
     3541 gatatgaggt atctgcaaat aaactaccta gttcatttac aaatgtaaat aaagaatcaa
     3601 gattagaggt gattttccgt ctattattat caaattcgtc aaatgctcca atagcaatta
     3661 gcggagaaat aaaaccactt ttttgaaact gctgtggcag cctcattaga aaatcttcaa
     3721 tactctgaaa cggttgattt ttagaaatcc aataagccaa atcgttagga acacccttaa
     3781 tattttttaa acctaaataa attttctttt tatagatgcg gtctctgtat gaaatagtat
     3841 taattgaagg tttttcaata acaaaaccaa aatcaatagc atcgattaga taatcactat
     3901 ttgaataatt tatcatgatg tcataaaaga catcggaata atgggctttg aaataggcta
     3961 attgaaaagc taaagccgaa taagcaaacg catggctgcg attaaaccca taacctgcaa
     4021 atttagccat gcgttcaaat ataagtcgag catcttcttg acgatgtccc ttagatactg
     4081 caccctctaa gaaggattga gacatctttt gcatctcctt agaatttttc ttagacatag
     4141 cacgtctgag caaatcagac ttgcctagcg taaaacctgc gtaggtctgg gcaatctgca
     4201 taacctgttc ttgatagagc attatgccat aggtaggttg taaaattgga gctatgacag
     4261 gatctactaa atcgattttt tctttaccaa aacgacggtt tataaaattc tcagtataat
     4321 cacttgcacc aggcctattc aagctggtgg tagctaccac atcttcaaaa cactctggcc
     4381 taatacgtcg caacaaatta atagctccac tttgctcaaa ttgaaaaatc ccctttgttt
     4441 gaccagctgc aaatagtttt agagtctctt tatcttctaa atcaatagtt tctaattgta
     4501 tagagatacc gtagtcctta tcgactttct ccttcatttt ctgaacaaat gtcaaatttc
     4561 gaagacctag aaagtccatt tttagaagac cattatcctc aacagaacta gcatcatatt
     4621 gcgttatcat catatcctca ccgtttttta aaggaatatg atctgttaac aaatcgtcac
     4681 tcattactac cccagcggca tgaatagaag tctgtctagg atttccttca atacgcttag
     4741 caatatcata ggctttttgg tattcaattt tactatctat tatttgtctg aaagctaagt
     4801 tgcggttata aacactggtc aaattatctc tgaaatgaat ttttttagtt atgtttgtta
     4861 actcatactc tgatgcacca aagcgtttaa aaacatctcg aatagcctgt ttcgcaccaa
     4921 aagttgagaa ggttacaatc tgtgcagaat gcattgaacc atatcgatta cgtacatagc
     4981 gcaagaattc tccacgataa atatccggaa gatcaatatc aatatctggc atcgaataac
     5041 gctcttcatt taaaaaacgt tcaaaaatta aattatgttt gacaggatcg ataccggtaa
     5101 tatttaaagc atatgagact aaacttcccg ctgctgaacc acgtcccatt cccatatagt
     5161 agcccttact acgaccaaat cttaataaat cccacacaat aaggaaatag tcatcaaagc
     5221 ccattttgtg aatgattgtt aattcttttt cgagacgctc ttgataagca tcgctccata
     5281 aacctttact ttttaagtta ttttgagtta aggttcttaa ttcttctcca gcaggaattt
     5341 ctctattaaa aataggtaac ttaaagtcag aatcaaagtg ataagcaaca ttctctacca
     5401 aagattctag gttagctaaa gcttcaggaa aacgttcctt gaatgctaga gtcatttctt
     5461 gacaaggttt taattcttga tttttgctcg catattgtgc gtcttttaac aggacattat
     5521 ctcttatagc atgaagcatc tgtaatgtct caatatcatt tttttgaaaa tatcgaacag
     5581 tatctatggc tataatgggt ttggtatatg gaatctgcgg agaggtcaaa ttaacaccaa
     5641 taacataatc aaagggtaaa ttgagctgat caagaacatc ttgataggga agaatcaatt
     5701 gcaccccttc aagatgttct gtcaataaat ttagagacat cttctcagac atttgagccg
     5761 ttgacaactt caagagattc ttataaccaa ctgtatttat cgcaatacag taaacttcta
     5821 ttgatctatt ttcaaccact atatcaaagc taaatccaac tattggtctt aggtttgcct
     5881 tttgcgcctc ttgaataaaa tgatatgctg cataaagatt actgtggtcc aagataccaa
     5941 tagtttgata tcctaatgat ttcgatttag agacatatgt ctttaaatca accaagctat
     6001 ccataaaaga ataaacggtt cttgtatcaa gttgtgcaaa catagtagcc cctttttacg
     6061 attttttact attatactat gaaacaataa acgattaaag tctcaatgat aattccttga
     6121 catttcaaat atttttttgt actattttta tagtacaaaa agtacaagat tatgaaagga
     6181 gttatctatg gcttgggaat tcaatgaaaa atcacctatt tattcacaaa ttgctgaaca
     6241 tataaagatg caaattgtta gtcaagaaat aaaaagtggt gaccaattgc ctaccgtgag
     6301 agagttagct caagaagctg gtgttaaccc aaataccatg caacgtgcct tcactgaatt
     6361 ggaacgtgaa ggaatggtat tctcacaacg tacttccgga cgctttgtta cagaagacaa
     6421 cttactaatt ggtaaaattc gtcaacaagt tgcaaaagca gagttagcaa cttttgtcaa
     6481 caacatgaaa aaaataggat ataaattaga tgaaattacg gttgctctcg atcattttat
     6541 aaaggagatt aattaaatga cacagttatt acaacttcat catgttacaa agaaatatca
     6601 caaacatact gctgttaatg atgtgactgt aagtattcca acaggaaaga ttataggttt
     6661 attaggtcca aatggtagcg gaaaaacaac aatcatcaaa atgataaatg gattacttca
     6721 gccagataag ggagatatcg ttattgatgg ttacagaccg tctgttgaaa ccaaaaaaat
     6781 tatttcatat ctccctgata cttcatattt acaagaaaat atgaaaatca aagacgtcgt
     6841 tacattattt gaagatttct ataacgattt tgattcaaag gtagcatatc aactatttga
     6901 ggatcttaac ctaaatccta gggaacgtct aaaaaaccta tcaaaaggga acaaggaaaa
     6961 agttcaatta atacttgtta tgagtcgtaa agctaggctc tacatccttg atgaacctat
     7021 tggtggtgta gaccctgcag cgcgtgacta cattttaaaa acaatcatta gcaactactc
     7081 taatgatgct tctgttttaa tttcgactca cttaatttct gacattgaac ctatccttga
     7141 tgaagttatc ttccttaaag aaggagaaat tgacctacaa ggcaatgctg atgacttaag
     7201 agaagagcac aactgttcga ttgatgctct ctttcgtgaa agattcaaga aataaaggag
     7261 attgatatgt ttggaaaatt attaaaatat gaattaaaat cagttggtaa gtggtatcta
     7321 acactcaacg ctgcggtttt attagtatct attattttag gactggtatt aaaggcactt
     7381 ggcggcaatt tttctactga tactaatagt actagcgccc aaatattcac tataatactc
     7441 gttttattgt tagcgatggt tatttcagga tctctgttat ccacccttgc tattattatc
     7501 aaacgttttt atagcaatat cttcggccgt caaggatatc taaccttaac gctcccagta
     7561 actacgaatc agattatttg ttcaaagcta ctagcttctc ttctttggag tatttttaat
     7621 atttttattg tcattattgg aatcatattg gtcatattac ctttagtagg tattggtcag
     7681 ttcgttgtcg cttttccaga aatctataaa attatttcat cttcgaacgc tccacttttt
     7741 atcgcctatt tctttctatc gtacgttgca gggacattac tgatttatct ctctatcgct
     7801 gtagggcaat tatttacaaa caaacgtgta ttgatgggaa ttgtttctta ttttggaata
     7861 tcactcttaa ttactttctt aacattaata atagatagta tttttcatat tgatttattc
     7921 aactctcatg caaatgcaac attctctcaa cctgtattgc tctacaatat acttgttagc
     7981 attgttgaaa ttgctatatt ctatatgttg acacactcta ttattaagta caaacttaat
     8041 atacaataaa aagattggag atatatctcc aacctttttt tatttttaat tcaaaaaatt
     8101 tttcaaaata tcaccgccat aaatccacaa atagctataa ccgatgatag aaataggttt
     8161 cgttatcata atgattgtca caaagcgctt aatagacata ttagatagac cggtaatcat
     8221 caccatgatg tccgctggtg aaatgggaga tgccatacaa aagataaaaa acttttcata
     8281 tccggatgtt tctagcttag actcatattt atcaaatgtc ttctgatcca taaatagcaa
     8341 aacgaatttt cgtccataaa atttgactaa ccaaaaaagg atgacacttc cgatgataat
     8401 cccaatatag ttataaataa agcctaaggt aggaccaaaa attaagaaac ctgctactgt
     8461 tgtaacacct ccaggaatta ctggaaaaac aatctgtaca atttgagcca caataaaaat
     8521 cattggaccc caaaattcat acttgtgtac taggtctttt aattcattgc tatcattcaa
     8581 aataccaagt ttgtacaaat aaacaactaa gacaagagtc gctattaacg ctaagacacc
     8641 caaagcctta ataaccttct gccaaaagcg gtaacgtttt gataatttca tattcatgct
     8701 ttaatcataa cacaactgtt aaatttttgc tattttaaat agtatttgtt atactatatt
     8761 ggtaagtttt tatgctttat tctacttttg gggtcgttac ggattcgaca ggcattatga
     8821 ggtatatttc gcgactcatc ggcagatgta aaatgccagt taaatataac tgcaaaaaat
     8881 acaaattctt acgcattagc tgcctaaaaa acagcctgcg tgatcttcac aagattgttt
     8941 gcgttttgct agaaggtctt atttatcagc aaactacgtt tggctactgt ctagttagtt
     9001 aaaaagagat ttatagactc gctatgtgag ggcttgagtt atgtgtcatc acctagttaa
     9061 atcaatacat aacctatagt tgtagacaaa tatattagca gatgtttgga cgtgggttcg
     9121 actcccaccg gctccatatc attttaccat aacttactat acatacttaa aaccttgtta
     9181 ttacaaggtt ttaagtatgt ttctttgttc atacattgcc tctatcttag gaaaaaaata
     9241 ctgggaacag actctaacat tgaattaggc cacctcatta gctcactctt tgtcaatatc
     9301 cgagaataag ttctttatca ttctaattta tagaatttat taaaaaaggt aaggttttta
     9361 gaggaaaaaa tgttataatc tatttagtct acgattggag gctagttatg gctagtattt
     9421 taaaaaaaca tttagagaaa cgtttagagc ttaaaaatga tcctatgagg acagatatga
     9481 gtactggtgc cagttaccct aaatatggat tctcggtagg aaagtacaca tatggatatc
     9541 aacaattctt ttacgaaggc gttaacttaa aagaaattgg agcattttgc tcaatagccc
     9601 agaacgttac tattactggt ctgaatcatc caaccgatca cattaccact aaccctttta
     9661 tttactataa gtcaagggga tttataaatg aggatagggc ggacttaata gatgaaaaga
     9721 aaaatggaaa agttattatt ggcaacgatg tctggatagg aactaatgtc actatacttc
     9781 catctgttac aataggtaat ggagcaatta ttggagcagg ttcagttatt accaaagaca
     9841 ttcctgacta tggtgttgtc gctggaacac ctgctaaaat tatcaaatat cgcttttcag
     9901 aggaagaaat aacattatta aatgcatctc agtggtggaa ttggtcagat gaagctatta
     9961 aggaacatat tagcgaattt tcagataaga aggaattctt caatactcta aaaagtattt
    10021 ccgaaaacaa aaatcataaa ctctagaata acttcatcaa atagcacact aaaaatacta
    10081 ctcagcataa tatgagtagt atttttagtg tgctattttg ctagtttatt tagcaccatg
    10141 tttagactaa tgcaattcta ggaaagctac ttagtctctc tgctattatc atgataagct
    10201 ccccaaaata tggttactaa taattttaaa gatttgttaa ataacctttt tttatcatca
    10261 tctagttctc taaagctatg aaaagctttc atcatttttg ctctggaagc caagttgaag
    10321 tcatccaaac tagatacgcc acttgatagg aagctatcaa ataataaatc aaaaaataat
    10381 tttctttctt gactttttaa ttcagccatc cactgttgaa atgtcttttc aaaacaaata
    10441 ctttttagcg atagtcctgt tccagtcttt aaaatagctg gttcttgaat ttcccaagtt
    10501 gtaacatcgt gttgcagaat tccacgcgca ttgctactaa taattagcgg ctctatatca
    10561 ctataaagca tcataccaac aatagattct tctggtagta agcgaatgca cttttgttta
    10621 gtagttttat actctgtagt aggtaacaaa gttttttgta gtcctgggga gtcaataagc
    10681 caaatcaaat caatttttgc ttttaaatct ggctttgtaa acattgcagc gtacaaggct
    10741 aaattacccc ccttagaatg acctgagaga actactttat caaaataagg cagtattgta
    10801 tttaagtatt taatcgctga acgatgagcc gatatttctc tcatataagt caatttaaaa
    10861 tcttctttcc aaccaataag gttggcatca gtaccacgaa atacaacctg atgataatta
    10921 atgttaggaa tagtaaaaac catagctgca aattgttttt caaattctaa actaatctca
    10981 gctcgataat aagataaagt taagttttta aaacgtttcg aggtcatcat taactgacat
    11041 aaatcatgtc gttgtgatgt gaacataaag ctatatgatt tagccatagt ttgaaggtac
    11101 tcatgatata actcacagac ta
//
LOCUS       DATRSD010000069        10465 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRSD010000069 DATRSD010000000
VERSION     DATRSD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.070"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            88..375
                     /locus_tag="VBD85_001543"
     CDS             88..375
                     /locus_tag="VBD85_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO7134090.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            387..878
                     /locus_tag="VBD85_001544"
     CDS             387..878
                     /locus_tag="VBD85_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7134091.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            923..1162
                     /locus_tag="VBD85_001545"
     CDS             923..1162
                     /locus_tag="VBD85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO7134092.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1332..2276
                     /locus_tag="VBD85_001546"
     CDS             1332..2276
                     /locus_tag="VBD85_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7134093.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     QNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2301..2972
                     /locus_tag="VBD85_001547"
     CDS             2301..2972
                     /locus_tag="VBD85_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO7134094.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3104..5932
                     /gene="uvrA"
                     /locus_tag="VBD85_001548"
     CDS             3104..5932
                     /gene="uvrA"
                     /locus_tag="VBD85_001548"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO7134095.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDMDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            6268..7767
                     /locus_tag="VBD85_001549"
     CDS             6268..7767
                     /locus_tag="VBD85_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7134096.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            7769..8485
                     /locus_tag="VBD85_001550"
     CDS             7769..8485
                     /locus_tag="VBD85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO7134097.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            8585..8986
                     /locus_tag="VBD85_001551"
     CDS             8585..8986
                     /locus_tag="VBD85_001551"
                     /inference="COORDINATES: protein motif:HMM:NF013486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEO7134098.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVT"
     gene            9038..9652
                     /locus_tag="VBD85_001552"
     CDS             9038..9652
                     /locus_tag="VBD85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7134099.1"
                     /translation="MLDFIKPDRTTELQVANFLDFRMRELGATGPSFDFIVASGYRSA
                     MPHGVASQKTIQSGETLTLDFGCYYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQA
                     IIDNVKSGMKRCDYDYLARQVIENSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIAS
                     GMVVTDEPGIYLDNKYGVRIEDDLLITETGCEVLTSAPKELIVL"
     gene            9664..10116
                     /locus_tag="VBD85_001553"
     CDS             9664..10116
                     /locus_tag="VBD85_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO7134100.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
BASE COUNT         3321 a         1697 c         2096 g         3351 t
ORIGIN      
        1 tttctactat aataatatct tgtgagcata acctcactta ctcatgaaaa attcatgagt
       61 cattagacca aaaggaggaa catatcaatg gctaaatacg aaattcttta tattattcgt
      121 ccaaacattg aagaagaagc taaaaacgct ttggtagcac gttttgattc tatcttatct
      181 gacaacggtg caactgttgt tgaatcaaaa gattgggaaa aacgtcgtct tgcatacgaa
      241 atccaagatt tcactgaagg actttaccat atcgttaacg ttgaagctga agatgcagta
      301 gctcttaatg agtttgatcg tctttcaaaa atcaacggtg acattcttcg tcacatgatc
      361 gttaaagtag actaagaagg tagtttatga ttaataatgt agtacttgta ggtcgcatga
      421 cccgtgatgc agaacttcgt tatacaccaa gtaatcaagc ggtagccact ttttcacttg
      481 cagttaatcg taattttaaa aatcaatctg gcgaacgtga ggctgatttt attaactgtg
      541 ttatttggcg ccaacaagct gaaaacttgg ctaactgggc aaaaaaaggt gctttggttg
      601 gaattacagg tcgtatccaa acgcgtaatt atgaaaacca acaaggtcaa cgtgtctatg
      661 taacagaagt tgttgcggaa agtttccaat tattggaaag tcgtgctaca cgagaaggtg
      721 gttcacctaa ctcttataat aacggtggct ataacaatgc tccgtcaaat aatagttatt
      781 cagcttcttc tcaacaaaca cctaatttta gtcgtgatga gagtccgttt ggtaactcaa
      841 atccaatgga tatttcagat gatgatctac cattctaata taatagatgg acagaattta
      901 aaactataaa ggagaaataa acatggctca acaacgtcgt ggcggattca aacgccgtaa
      961 aaaagttgat tatatcgcag ctaacaagat tgaatatgtt gattacaaag atacagagct
     1021 tcttagccgt ttcgtttcag aacgtggaaa aattttacca cgtcgtgtaa caggaacttc
     1081 agctaaaaac caacgtaaag taacaacagc aatcaaacgt gctcgtgtta tggctcttat
     1141 gccttacgta aatgaagatt aatataaaat agccctagcg gctatttttt tattcttttt
     1201 accattatta ttggtattta cgagtcaaat aatagaaaaa tttaggatac caccgtatta
     1261 accgtgcaaa acaaatggtt ttcctgtata atattaaggt atggattttt tataacaaag
     1321 gaggaatcgc catgaagcaa atgtttcttt caacggcaat tgaatttaaa gaaattgaaa
     1381 cattcgagcc aggtgcttgg ataaatctgg ttaatccctc ccaggaagaa tccgaacaag
     1441 tagctgatca atttggtatt gatattgatg atttacgtgc tcccctcgat gtggaagaaa
     1501 cttcccgtat ctcggttgaa gatgattata ctttagttat tgttgacgtt cctacctatg
     1561 aagaacgcaa taacaaaagt tattatatga ctattcctat gggcattatc gttactgaca
     1621 acgctgttat tactacttgc ctagaacatt tgacgctgtt tgatcacttt tatagacgac
     1681 gtgtcaaaaa tttttatacc tttatgaaga cacgttttgt tttccaattg ctttatcgca
     1741 atgccgaatt atacttacag gctttgcgta ctatcgatag gcagagcgat aaaattgaag
     1801 cacaactaga gagtgcaact cgaaatgagc aattgatcga tatgatggaa ttggaaaaat
     1861 ctattgtcta tctaaaggcc tctttaaaat ttaatgagcg aatcgtcaaa aaattgacca
     1921 gtagtaccag tagtttaaaa aaatacattg aagatgaaga tctgcttgaa gatacattga
     1981 tcgaaactca gcaggctatt gaaatggcaa atatctatga aaatgtctta aatgccatga
     2041 cagaaacgac tgcttcaatc atcggtaata atcagaatac cattatgaaa accttagccc
     2101 tagtaactat gacattggat attccaacgg ttattttttc ggcgtatggg atgaactttc
     2161 aaaataattg gatgcctttg aatggtctcg cacatggctt tatctatgtt gttcttttgg
     2221 cctttttgat gagttcattt gttgtatttt attttattcg taaaaaatgg ttttaatagt
     2281 atattaatca aggagttcac atgactttac aagacttaac taaaaagaat caagagtttg
     2341 ttcatattgc aactaatcaa cttttagctg atggaaaatc agatgcagaa atcaaagcta
     2401 ttttagaaga acatctccca gagattatag ataatcagaa aaaaggaatt accgcacgct
     2461 cactcttagg agcaccaact acttgggcag cctcattcac tgagcgacct gaggataaag
     2521 ctagagtatc tgtacaaaaa aataccaatc cttggttgat gtggttagat acttccttac
     2581 tatttcttgg cttagtaact gctttgaatg gtttaatgtt gctatttggt caatctaatg
     2641 tcaatacagg gcttatttca attttaactc ttggttttgg tggtggtgcg gccatgtacg
     2701 ttacttatta ctatatttat cgccatatgg gtaaaccaaa atctgaaaga ccaggctggc
     2761 ttaaatcttt cgcagtactg gcactagtta tgttagtttg gtttgcctta tttgcagtag
     2821 tacctcttct acctgcaact attaatccta aacttcctga ggttgttttg tttattattg
     2881 ctttagcatc gtttggtttg cgcttttatt tacaacgtaa atataatatt caaagctcaa
     2941 tggcaccagt ggcaccacaa caaagaagat aataacaaag agtatgaacg tattagttca
     3001 tctctttttt ctatgaaatt taaaactaaa aaaatactga ctttattaaa atttgatata
     3061 ataagtcaac actattcaaa aaggagtgct gaggcacaac attatgcaag ataaattaat
     3121 gataagagga gcaagagctc acaatttaaa aaatataagt gtcgacattc cacgagacaa
     3181 gttagttgtc gttacgggac tatcaggatc aggtaagtct agtttggcct ttgacactat
     3241 ctatgcagaa ggacaacgtc gttacgttga aagtctttct gcttacgcgc gccaattttt
     3301 gggaaatatg gaaaagcctg atgtagattc tattgatggc cttagtccag ctatttctat
     3361 tgatcagaaa acaacaagta agaatcctcg ctctactgtt gggactgtaa cagaaattaa
     3421 cgattattta cgtttgcttt atgctcgtgt tggaacccca tattgtatca atggtcacgg
     3481 agccatcaca gcatcttctg tagagcaaat tgtggataag gtactagcgc ttccagagcg
     3541 aacaaagatg caaattttag ccccaattat acgtcgaaaa aaaggacaac acaaatcaac
     3601 atttgagaaa atacaaaaag atggttacgt tcgtgttcgt attgatggtg atattcacga
     3661 tgttactgag gtgcctgaat tatcaaaaag taagatgcac aatattgata ttgtagtaga
     3721 tcgattaatt aataaagagg gcattcgttc ccgcttattt gattccgttg aagcggctct
     3781 acgtttgtcg gatggttatg tcgttattga tacaatggat ggcaatgagt tattgttttc
     3841 agaacattat tcttgtcctg aatgtggttt tactgttcct gaattagagc cacgcctttt
     3901 ttcttttaat gctccctttg gttcttgtcc gacctgtgat ggcttaggaa ttaagttaga
     3961 agtagatata gatttggtta ttccagatag aagtaagacc cttagagaag gggccttagt
     4021 tccttggaat ccaatttcat caaattatta tccaacgatg ctagagcaag ctatgactca
     4081 atttggtgtt gatatggata cgccatttga aaaattaagc aaggcggagc aggacctagc
     4141 cctatatggt tcaggcgaac gagagttcca tttccattat attaacgatt ttggaggcga
     4201 gagaaatatt gatttgcctt ttgaaggggt tgtcaataat attaatcgtc gttaccatga
     4261 aactaatagc gattacacac gcaatgttat gcgagaatat atgaacgagc taaaatgtaa
     4321 cacttgtcat ggctatcgtc tcaatgatca agcactttgc gtaagagttg ggggagaaga
     4381 aggtcttaat attgggcaag tctcagattt atcgatagca gaccatttag aacttttaga
     4441 aacattgcgt ttatcttcaa atgaacaatt aattgctcga ccaattatca aagaaatcca
     4501 tgaccgtcta agtttcttaa ataatgttgg acttaattat ttaaatctgt cacgttctgc
     4561 aggaacactt tccgggggcg agagtcaacg tattcgtttg gctactcaaa ttgggtctaa
     4621 tttatcaggt gtcctttatg tacttgatga gccgtccatt ggacttcatc agcgagacaa
     4681 tgaccgtttg attgatagtc ttaagaaaat gcgtgatttg ggtaatacgc ttattgttgt
     4741 tgaacatgat gaagatacaa tgatggcagc tgattggtta atcgatgtgg gacctggcgc
     4801 tggtgcgttt ggtggtgaga ttgtggcctc tggtacaccg aagcaagtag ctaaaaatac
     4861 taaatctatt acaggacaat atttgtcagg taagaaagtg attcctgtgc catctgaacg
     4921 aagagtaggt aatggtcgct ttttagaaat caaaggtgcg gcagaaaaca acctacaaaa
     4981 tttagatgtc aagttccctc tcggtaaatt tattgccgta actggtgtct caggttctgg
     5041 aaaatcaacc ttaattaata gtattttgaa aaaagctgtt gcgcagaagc ttaaccgtaa
     5101 ctcagataaa ccaggtaaat atgtttcttt agaaggcatt gaatatgttg atcgattgat
     5161 tgatattgat caaagtccaa ttggtcggac tccacgttct aatcccgcaa cctatacagg
     5221 tgtttttgat gatattcgtg atctttttgc tcaaactaat gaggcaaaga ttcgaggtta
     5281 taaaaaagga cgcttctcat ttaacgtcaa aggtggacgt tgtgagtctt gttctggcga
     5341 tggtatcatc aaaattgaga tgcatttttt accagatgtc tacgttcctt gtgaagtatg
     5401 ccacggcacg cgctataata gtgagactct ggaagtacat tacaaagaaa aaaatattgc
     5461 acaaattctt gatatgacag tgaatgatgc agttacattt tttgcagcta ttcctaaaat
     5521 tgctcgtaag ttacagacta ttaaggatgt tggtttggga tatgtcactt tggggcaacc
     5581 agctacaacc ttatcaggag gagaagctca gcgaatgaag ctggcaagtg aattacataa
     5641 gcgttcgaca ggtaagagtc tttacatatt agacgaacca acaacggggc ttcatgcaga
     5701 tgatattgct cgtttactta aggttttgga tcgttttgta gatgatggta atacagtgct
     5761 tgttattgag cataaccttg atgttattaa aactgctgac cacattattg atcttggccc
     5821 tgaaggaggt attggtggcg gacaaattgt agctatcgga acaccagaag aagttgctga
     5881 aaatcctaaa tcctatacag gatattattt aaaagagaag ttagcaagat agtttattgg
     5941 gagctgagta atattactca gttccttttt gtaagctaac attgcgtaga agtcattaaa
     6001 tggagaacgc tttattagga aaaatagtgg ggagatattt taaaaaaagg aaataatgat
     6061 gttttgaaaa aaatagtttc tatgagaatc tttttcttga caataaaatt tagctattat
     6121 aaaatagttc tcacgataac taaaataatg attttttcag aaaataatat tatttcttga
     6181 ttaaaaaatc aaaaaataac cttataagac ctatttgtta ttcttaaaag atattagtta
     6241 aaatcagaaa tagagattgg agataaaatg actacaaaag caaaacatga tattcatggc
     6301 aaaccatata atcgtacagc catgatcacc ttattgttga ttgctacatt tgcaggggta
     6361 ttaaatcaga caagtttggg aacggctatc ccaactttga tgaatagctt taacatttcg
     6421 ttgtcaacgg ctcaacaagc aacaacatgg tttttattag cgaatgggat tatgattcct
     6481 gtttcagctt atttagcgac acgtttttca acaaaatggt tgtatgtgac atcctacgtt
     6541 gttttactta ttggactgtt aatgacgacg ttagcaccta catctaactg gaaccttttt
     6601 ttagtaggac gtattattca agcgatttct gtagggattt ctatgccttt gatgcaggtt
     6661 gttatggtga atgtttttcc tccagagcag cgtggtgccg ctatgggact taatggtttg
     6721 gtggttggtc ttgccccagc tattgggcct acattagcag ggtggatttt aaaacaagaa
     6781 tttcattttg cagggcatga tttaacatgg cgtgcaattt tccttcttcc tttactaatt
     6841 ttaacggtca ctacaatttt atcccccttt gttctaaaag atgtggttga taataagtca
     6901 gttaaattgg aagtgccttc ccttatcctt tcaataattg gctttggtag ctttttgtgg
     6961 ggcttcacaa atgtggcaac ttatggatgg ggagatattg gatatgttat ttctcctatt
     7021 atggttggta ttatttttat cgccttattc attcatcgtc aattaaaact agaaacaccg
     7081 ttcttagata tccgtgtttt caaaaataaa caattctcag taacaacagc agctattgca
     7141 ctttcaatga tggcgatgat gggtgttgaa atgatgctac cgctttactt gcaaaatgtt
     7201 catggtctct ctgcacttga ttctggttta gctttattac caggtgcctt gatgatgggg
     7261 atagttagtc caatttctgg agctgtatat gataaagttg gtgcaagacg tatggctatg
     7321 attggtttta ccatactagg tgtagcgacg ttaccttttg ttttcttaac gactacaaca
     7381 ccagatcatt ttattaccct cttatatgca gtacgtatgt ttggtattgc tatggttatg
     7441 atgccattga cagcaagtgc gatgagcgca cttccaccac atgaggccgc acatggaaca
     7501 gcagctaata atactgctcg tcaaattgct tcagcagttg tagttgcatt gctttcaagt
     7561 gttgctcaaa atattatcac caacaataaa ccatcaaaag atttgctaac aatgaatcct
     7621 ttaaaatatg caaatcagat gttgaatgct agtcttgatg gcttccatgt ttcttttgcc
     7681 attggttttg tatttgcggt tcttggttta ctggtatctc ttttcctgag aaaagggaaa
     7741 attattgaga cggaaaagga ggtatagcat gattattttg attattgcac tggcaactat
     7801 tttgacattt gtcacatgga tgttgattcg tccaaaagct ctacggtata ttctaggtct
     7861 tctctcatta gctttgttgt ttgcctcagt ttggatgtta acggatcact ttattaatca
     7921 tacaggtatg actattgaaa ctaagacaaa gacacgtgag atttatagtg cgggtgatag
     7981 taaggctcca tttggtctta tgatttataa agagattggt actcattcga gtaataatgt
     8041 tttagtttat cgaacagatt taaatcaaaa agaagcaaaa gctcactttg ttccaaatac
     8101 aaaagaagtg acaagtgctg tgaaaaaatc tgctagctac caattagctg atgtgaaaaa
     8161 agccacagta acaacaaaga ctaaacgtta tgtgtggaaa gatagtcttt cgaaactact
     8221 ttatggcttt gcaggtgaaa atggagaatt gatttctcag aaatcagttg ctaaaatacc
     8281 acgaaaaact tggctagttt tgactaaaga gcagtccgaa cacttagaat cggctgcggc
     8341 taaactaaaa gaacaaatga cttcgcaagc aaaagtaaac ccagtgaaag ctatggagat
     8401 aaagacatta gctaagacaa atccagaagc ttatgctgag atgcaagtta aagcattaaa
     8461 agaagctctt aatataaaag aatagtaaag taaaagcaac tgaaatgagt tgcttttctt
     8521 atagctaaat gttttaaaag atgataaaat taacctaatt aaggttagaa tagcgaggtt
     8581 tcttatgggt tttgaacaac gtctaactag atgtcaaaca gcaatttcac aattatcatg
     8641 tgacgcactc ttaataacaa atttaacaaa tattttttat ttgactggtt tttcaggaac
     8701 taatgcaacg gtactcatta gtccaaaaca ccggattttt gttacagatt cacgatatgc
     8761 cttgatagca aagaatactg taagagagtt tgatattatc attagcagag aaccacttgc
     8821 ggctattttg aaaattattc gcgatgacgc attgatagcg attggttttg aaactgatat
     8881 ttcttatcat atgtacaagc atatggtgga agtttttgaa gattatcgct tgatagaagc
     8941 tccatctgtt gttgaaaagt tacgtatgat taaagatgta acataacttg cgatcatccg
     9001 tcaagcctgt cgtatttcgg accaagcttt tttagatgtg ttggatttta taaagcctga
     9061 tcgaacaact gaattgcaag ttgctaattt tttagacttt cgcatgagag aattaggagc
     9121 aactggtcct tcatttgatt ttattgtagc atctggatat cgatcagcga tgccacatgg
     9181 ggttgcgagt cagaagacta ttcaatcggg agaaactctg actttagatt ttggttgtta
     9241 ctatcagcac tatgttagtg atatgacacg tacgattcat atcggtcatg tcactgatca
     9301 ggaacgcgag atttacgata tcgttttgaa aagtaatcaa gctattattg ataatgtaaa
     9361 atcaggaatg aagcgttgtg attatgatta cttggcgcga caagtcattg aaaattctgg
     9421 atacggtaat cattttaccc acggtatagg gcatggtatg gggcttgatg ttcatgaaat
     9481 tccttatttt ggtaagtcag agggagtgat tgcatctggg atggtagtga ctgacgagcc
     9541 aggaatatat ctcgataata aatacggtgt tcgtattgaa gatgatttat taataacgga
     9601 aacaggttgc gaagtattga catctgcacc taaagaatta attgtacttt aaaggaaaat
     9661 agaatgaata gactttcttg ggaagattat tttatggcaa atgcagagtt gatttctaaa
     9721 cgttctacct gtgaccgtgc ttttgttgga gctgttttag tcaaaaataa tcgcatcatt
     9781 gctacaggtt ataacggagg agtctctgag acagataact gtaacgaagt gggacattat
     9841 atggaagatg gtcactgtat taggactgtt catgctgaga tgaatgccct tattcagtgt
     9901 gctaaagaag gtatttctac taataataca gaaatttatg tgacgcattt tccttgtatc
     9961 aattgcacca aggcgctctt gcaagccggg gttaagaaaa ttacttataa agctaattat
    10021 cgcccacacc catttgctat tgaacttatg gaagcaaaag gagttgctta tgttcaacat
    10081 gatgttccag aggtaacact agggatggaa gagtaaagag gaagatagag tatatgccta
    10141 tcttccttga aggttttatc gtttaagcta agggccagtt atgttaaata tattgaacga
    10201 gataattctc aaaattggtc aattgttagt catgggatta ctgaaaatgg cctaaaaaag
    10261 gtgatcacca gttaatagtc attattccta atgatttttc acagaaagtt ttagatgttg
    10321 ataatagaat agctgatcca tctattatca acatctaaaa ttagtactgc tgggaatctc
    10381 gaattgcaaa aataatttac aaaaaagaga aaagatattg ttgctgacct caatagatgt
    10441 aaatccagtt tatgctaata tggca
//
LOCUS       DATRSD010000070        10270 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRSD010000070 DATRSD010000000
VERSION     DATRSD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.071"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            189..674
                     /locus_tag="VBD85_001554"
     CDS             189..674
                     /locus_tag="VBD85_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134101.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQLQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            773..1426
                     /locus_tag="VBD85_001555"
     CDS             773..1426
                     /locus_tag="VBD85_001555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO7134102.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1492..2130)
                     /gene="plsY"
                     /locus_tag="VBD85_001556"
     CDS             complement(1492..2130)
                     /gene="plsY"
                     /locus_tag="VBD85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO7134103.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGITTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            2244..4193
                     /gene="parE"
                     /locus_tag="VBD85_001557"
     CDS             2244..4193
                     /gene="parE"
                     /locus_tag="VBD85_001557"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO7134104.1"
                     /translation="MAKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNIDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            4327..6786
                     /gene="parC"
                     /locus_tag="VBD85_001558"
     CDS             4327..6786
                     /gene="parC"
                     /locus_tag="VBD85_001558"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO7134105.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIAGYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYALRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEGVFSVKIK"
     gene            6899..7921
                     /locus_tag="VBD85_001559"
     CDS             6899..7921
                     /locus_tag="VBD85_001559"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO7134106.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            8004..8234
                     /locus_tag="VBD85_001560"
     CDS             8004..8234
                     /locus_tag="VBD85_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO7134107.1"
                     /translation="MSKKDKKIEIQISDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            8295..8375
                     /locus_tag="VBD85_001561"
     tRNA            8295..8375
                     /locus_tag="VBD85_001561"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8329..8331,aa:Tyr,seq:gta)
     gene            8381..8452
                     /locus_tag="VBD85_001562"
     tRNA            8381..8452
                     /locus_tag="VBD85_001562"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8413..8415,aa:Gln,seq:ttg)
     gene            8578..9780
                     /gene="rpsA"
                     /locus_tag="VBD85_001563"
     CDS             8578..9780
                     /gene="rpsA"
                     /locus_tag="VBD85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO7134108.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            9853..9924
                     /locus_tag="VBD85_001564"
     tRNA            9853..9924
                     /locus_tag="VBD85_001564"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9886..9888,aa:Arg,seq:ccg)
     gene            complement(9970..>10270)
                     /locus_tag="VBD85_001565"
     CDS             complement(9970..>10270)
                     /locus_tag="VBD85_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7134109.1"
                     /translation="TIKLLDQYRLLFKTETRIFEGISNTAVNKTLKKLVGRNIHIHSL
                     RHTYVSHLISEGIDLFAISKIVGHKDLNITLSTYAHLLEDTKNKNNEIIRELFGA"
BASE COUNT         3400 a         1608 c         2081 g         3181 t
ORIGIN      
        1 cattttactc aagaggctaa tgatttgtta caaagtcaat atcaattatt tgtagacgaa
       61 gttaaaacct tataatattt attttataaa attattcatc ttatttttgc gatttcaata
      121 gtgatatggt atatttaaag taagctacgt atagttttac cagtcagaaa tagtaaggat
      181 ggttgagtat gagagaaacc gaaacacacg atcaccaagc gctaattcaa aaattgttag
      241 tcagtattca ttatttaacc ttgtttcgtg atgaaattat attagtagaa aaaaccccat
      301 ctttactggg gaaacatttt tctattgcta ttgtgcagaa tgaactaggt gagatacttt
      361 ctaaaataga agctttgagt aagcaaaaaa aactgattcg ttctatttat tggtatgatg
      421 aatcgtcatt taaagtcatg aataaggctc tggctattgt agaagaatgg attaagggct
      481 tagataatct tttagaattc tgtcaattac agacggtttt tcaagcaatt ttaggtgatg
      541 aacgtgcaca tgtatttgga atccttattg atgtttatac ttctttaaac attataaata
      601 cttctttgaa agaagaaaat tctagaccag tgtccttatc tgatttggct ctaggtctta
      661 aggacgaaga ttaataaaaa aacgttactg ctttgaatga ataaagcagt aacgtttttt
      721 ataaaatatt gctatactag taatacagat atctaaaagt aaggagttat aaatgaaaca
      781 ttctagttgg catgatttaa taaaaagaga actacccaat cattactaca ataaaattaa
      841 tacatttatg gatgcagttt acgaaagcgg aatcgtttat cctccaagag ataaggtgtt
      901 caatgccata cagataacac cactagaaaa cgtaaaagta gtgattattg gccaagatcc
      961 ttaccatggc cctcaacaag ctcaaggact ctcattttct gttccagaca atttaccagc
     1021 tcctccttca cttcaaaata ttctaaaaga attagcagaa gatattgggt cacgttcaca
     1081 tcatgactta acatcatggg cgcaacaagg agtgctgcta ttgaatgctt gtttaacagt
     1141 tccagaacac caagctaatg gtcatgctgg gcttatatgg gaacctttta ctgatgctgt
     1201 catcaaagtg gttaaccaaa aggaaacacc agtagtcttt attctgtggg gtggctatgc
     1261 tcgaaagaaa aagtcgctaa ttgataatcc tattcatcat attattgaga gtcctcatcc
     1321 gagtccctta tctgcttatc gtggcttctt tggtagcaga cctttttcta gaacaaatca
     1381 tttcttagaa gaagaaggaa taaatgagat tgattggtta aattagaagg gaaatctact
     1441 ctcgaaacat tgttagtttt ataataaaaa accgctacta gcggtttttt attatttatt
     1501 tttttgttta cttaggttta agccaaacgg aaccaaattt tcttgccttt ttcgaatacg
     1561 tttgatatta tcctgatgtc ggataataat cgtgagagcc attaagataa ccacagtggt
     1621 aaatatccag tcgtagtccg ttaatataaa gcctactaat ggaaaaatta agacactaag
     1681 aatacctacg acagctactg taatactgga tagggaaatc atgctaaaaa gataaagcgt
     1741 taataaaaag ataactaata agtaaaggaa aaaagaaggt gcaaatccta gcaaaacccc
     1801 agcgcttgta gcaacggctt tcccaccttt gaattgtgca aaaataggga aagtgtgacc
     1861 tataatagca aaaaaaccga taaaaaatgg agatactgtc gttataccta atatgatagg
     1921 aataagtgtt gctagagtcc cttttaaaat gtcaatagtc aaagtgacaa tgccagcttt
     1981 tacacctaaa atacggaagg tattcgtggt gccagtatta ccactaccgt gttgacgaag
     2041 attgacttgg taaaaatatt taccaatcca aagtccagtt tggatagagc ctaacagata
     2101 ggcaatgata atcataatta taatattcat acccttatta taacataagc ataaaagtct
     2161 ttttgctatt tttaaaaaaa taattttgca aaaacagcaa aaaacttgta atataaaaaa
     2221 gatgaaaaaa tttggaggtc actttggcta aacaagatat aacagtgacg aattatggcg
     2281 acgatgctat tcaagtctta gaaggattag atgctgttcg taagcgtcca gggatgtata
     2341 taggttccac agatggaact ggtttacatc atttagtgtg ggaaattgta gataatgcag
     2401 ttgatgaagc tctatcaggt tttggaaatc gcattgatgt tattattaac aaagatggga
     2461 gtataactgt tacagaccat ggacgcggta tgccaactgg aatgcatgct atgggaaaac
     2521 ctactgttga agtcatcttc acggtgcttc atgcaggtgg aaaatttgga caaggtggtt
     2581 ataaaacatc aggtggtctc catggtgttg gatcatctgt agttaatgct ttgtcttctt
     2641 ggttagaagt tgaaatcatt cgcgacggag ctatttacag acaacgtttt gaaaatggtg
     2701 gtaagcccgt tacaaccctg aaaaaaatcg gcacggcacc aaaatcaaaa tctgggacat
     2761 ctgtcagctt tatgccagat cagtcagtat tttcaacgat agattttaag tttaatacaa
     2821 ttgcagagcg cttaaaggaa tctgcctttc tcttaaaaaa tgttacttta accttaacag
     2881 ataatcgttc agaagaagca gagcatctag aatttcatta tgaaaacggt gttcaagatt
     2941 ttgtagagta tttaaatgaa gataaagaaa cgttgacacc aatcatgttt ttcgaaggtg
     3001 aagaacaaga atttcatatc gaagttgctt tgcagtataa tgatggtttt tcagataata
     3061 ttctttcttt tgtcaataat gttcgtacta aagatggtgg aacacatgaa actggcttga
     3121 aatcagctat tacaaagtct atgaatgact atgcacgtaa aacaggactt cttaaagaaa
     3181 aagataaaaa ccttgaaggt tctgattatc gtgaggggct atcagccatt ttatctattc
     3241 ttgtacctga ggagcacttg caatttgaag gccaaactaa agataaacta ggaagtccct
     3301 tagcacgacc aattgttgat ggtattgttt ctgaaaagtt aacttacttt cttatggaaa
     3361 atggagattt agcatctaat ttaatccgta aggcaataaa agcacgtgat gcgcgtgaag
     3421 ctgcacgtaa agctcgtgat gaaagtagaa atgggaaaaa gtcgaaaaaa gataaaggtc
     3481 tactttctgg aaaattaacc cctgcacaat caaaaaatgc taagaaaaat gaactctatt
     3541 tagtcgaagg agactctgca ggtggctctg ctaagcaagg tcgtgataga aagtttcaag
     3601 ctattttacc gttgcgagga aaagtattga atacagctaa ggcaaagatg gctgatatta
     3661 taaaaaacga agaaattaat acaatgatcc ataccattgg tgcaggtgtt ggtcctgatt
     3721 ttaatattga cgatattaat tatgataaag ttatcattat gaccgatgct gatactgacg
     3781 gagctcatat tcagacacta ttattaactt tcttttatcg ctatatgaga cctcttgttg
     3841 aagagggaca tgtctatatt gctttgccac ctttgtataa aatgtcaaaa ggaaaaggta
     3901 agaaagaaat tgttgagtat gcttggacgg atatagagtt agaagaatta cgacaaaaat
     3961 ttggtaaagg ctcactatta caacgatata aagggcttgg ggaaatgaat gccgatcaat
     4021 tgtgggaaac cactatgaac cctgagacaa ggacattaat tcgtgtaaca attgaagatt
     4081 tagcaagagc agaacgtcga gttaatgtct tgatgggtga taaagttcct ccaagacgcc
     4141 aatggattga agataatgtc aagtttactt tagaagagaa tacagtattt taataaagag
     4201 tcttggttat tcatgtttta tagtgtacaa gtaatattgt atgcgttaga caagagagcc
     4261 aatcattcat ttcaaataag tggaaaaata atactaagcc actccaacta ggagaaaatt
     4321 aaaaatatgt ctaatattca aaatatgtcc cttgaggaca ttatgggaga gcgttttggg
     4381 cgctattcta agtatatcat tcaagaacgg gcgttgccgg atattcgtga tggcttaaaa
     4441 cctgttcaac gtcgaatcct ttattcaatg aataaagatg gtaatacttt tgaaaaggga
     4501 tttcgcaaat ctgcaaaatc agtcggtaat gttatgggga atttccatcc tcatggggat
     4561 tcctctatct atgacgcgat ggttcgtatg tctcaagatt ggaaaaatcg tgagacattg
     4621 attgaaatgc acggaaataa tggttctatg gatggagatc ctgcggcggc tatgcgttat
     4681 acggaagccc gtctatcaga aattgctggt tatcttcttc aagatattga taagaatacc
     4741 gtaccctttg cctggaattt cgatgataca gagaaagaac caactgtttt accagctgcc
     4801 tttcccaatc ttttagtcaa cggagcaaca ggaatatctg caggctatgc aacagatatt
     4861 ccaccacata atttagcaga agtcattgat gctgtcgtgt acatgattga tcaccctaaa
     4921 gctaaattag ataaattaat ggaatttcta cctggtccag attttccaac tggcgctatc
     4981 attcaaggaa aagatgaaat tcgtaaggca tatgagactg gtaaggggag agtagcggtt
     5041 cgctcgcgaa ctgctattga aaccttaaaa ggtggtaaga aacaaattat tgttactgaa
     5101 attccttatg aagttaataa gtcagtttta gtaaaacgaa tcgatgatgt tcgtgttaat
     5161 aataaagttc caggaatagc agaggtgcgt gatgagtctg atagagatgg tcttcgtatc
     5221 gctattgagc tcaaaaaaga agccgatgaa acaattgtct taaattatct ttttaaatat
     5281 acagacctac aagtgaatta taatttcaat atggttgcta ttgatgacta cacccctaag
     5341 caggtgggtc tatcaagaat attaactagc tatatcgctc accgaagaga aattattatt
     5401 gctcgttcaa aatttgataa agaaaaagcc gaaaaacgtc tccatattgt tgagggactg
     5461 attagggttc tttctatttt ggatgaagtg attgcattaa ttcgtgcttc tgagaataaa
     5521 gctgatgcga aagagaattt gaaggtcagc tatgagtttt cagaagccca agcagaggct
     5581 atcgtcacat tgcaattata tcgtctcaca aatactgata ttgtgacgtt gagagaagaa
     5641 gaagaagaac ttcgtcaaca aatcacaatg cttaaggcaa ttattagtga tgaacgtacg
     5701 atgtataacg taatgaaacg tgagttacgt gaggtcaaaa agaaatttgc caatactaga
     5761 cgttctgaac ttcaagaatt agctgaaact attgaaatcg atactgcaag tttaattatc
     5821 gaagaggaca catacgtcag tgttactaga ggcggatatg taaaacgcac cagtcctcgc
     5881 tctttcaatg catcaactgt tgatgaactt ggtaagcgag aagatgacga attgatattt
     5941 gtgagcaatg caaagactac ccaacattta ttgatgttta ctaaccttgg taatcttgct
     6001 tatcgtccag ttcatgaatt agctgatatt cgatggaaag acgttggtga acatttatca
     6061 caaaatcttg ttaactttgc ttcaaatgaa gaaattattt atgctgaatt agtagatgat
     6121 tttactaaag aaacttattt tgctgtcact agcttagggc aaataaagcg ttttgaacgt
     6181 caagaaatca gtccatggcg gacatataag tcaaaaacag ctaaatatgc taagttaaaa
     6241 aatgtagatg attacgttgt gactgttgct cctatccaat tagaggatgt tatacttgtc
     6301 acttataatg gttatgcgtt gagatttagt ataaatgatg taccagtcgt tggtagtaaa
     6361 gccgcaggtg taaaagcgat gaatttaaaa gatagtgatc acatcgtatc tgcatttata
     6421 gctaacacca caagtttata tttattaact catagaggaa gtcttaaacg aatggccatt
     6481 gatgttatcc ccacaactag tcgtgctaat cgaggacttc aagttcttcg tgaattgaaa
     6541 tccaaaccgc atcgcgtttt taaagcagga cctgtatatt tagaggattc atcttttgaa
     6601 tttgatttat ttagttcagt atcgaatcat gaaggagata ctttcgtact tgagattatg
     6661 tctaaaacag gaaaagttta cgatgttgat ttaagtcaat ggagtttttc tgaacgtaca
     6721 agtaatggct cttttgtatc tgataagatt tctgacgaag gagtattctc agttaaaatt
     6781 aagtaaaaca catttgtgtt tttactagtt tggtaatttt tctgaatttt acaaataata
     6841 ttgaaaaaat aatgataata gcgtacaata gttagaaacc caaagaaaga ggtaccttat
     6901 gacagtaaat ttagattggg ataatttagg ttttgcttac agaaaattac cattccgata
     6961 catttcgcat tttaaagatg ggaaatggga tgatggcaag ttgacagatg atgcaacgct
     7021 acatatttca gaaagttctc ctgccctcca ctatgggcaa caagcttttg aaggattgaa
     7081 agcttataga actaaagatg gctctattca gttatttcgt ccagatcaaa atgcagaacg
     7141 acttcaaaga acagcagatc gtcttttaat gccacatgtt ccgacagaca aatttattgc
     7201 agcggttaaa tctgttgtac gcgctaatga ggagtttgtt ccaccgtatg gtacaggggc
     7261 aacattatac attcgtccct tattaattgg tgtgggagat attattggag ttaaacctgc
     7321 tgaagaatat atttttacgg tatttgcaat gccagtaggt agttatttta aaggtggttt
     7381 aacaccaact aactttattg tttccaaaga atatgataga gcagcaccga atggtacagg
     7441 agctgcaaaa gttggtggta actatgctgc tagtcttctt cctgggaaat acgctcatga
     7501 gaaacaattt tcagatgtta tctatcttga tccagcgaca catactaaaa ttgaagaggt
     7561 tggagcagct aatttctttg gaattactaa agataatcag tttatcaccc ctttgagtcc
     7621 ttcaatttta ccttcaatta ctaaatattc attgctttac cttgctaaag aaaggtttgg
     7681 tatggaagcg attgaaggag atgtctttgt cgacgaatta gataaattca cagaagctgg
     7741 cgcatgtggt acagccgcag ttatttcacc aattggagga attcaaaacg gagatgactt
     7801 ccatgtcttc tacagtgaga cagaagttgg tccagcgaca cgtaaactat atgatgagtt
     7861 agtagggatc cagtttggtg atgttgaagc accagaaggg tggatttata aagtagatta
     7921 agtctatact aaaagcttgc tcttgcaagc ttttagtatt ttgtgtacaa taaaaaacaa
     7981 taaaagagat attggagtta atagtgagta aaaaagataa gaaaattgaa attcaaattt
     8041 cagacgctca agtaacagtt aatggaacta aggttgatgg ataccaactc gttattggaa
     8101 aaaaaattgt aggtcaaatt gcagagctag attctaaatt tgctgtgatt aaaggtgagc
     8161 aagtgagtgg attctataaa aatttagatc aagctatggg aaatattatt gaagaatata
     8221 atcttaatcg ctaatattta tttaaaaagc ttgcgtgcta acaacatctg tggtagaata
     8281 gtcagtgttg ttacggaaag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc
     8341 cttcgggttc gggggttcga atccctctct ttccattatt tggggtatag ccaagcggta
     8401 aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc agtcaaaggt
     8461 atcaaacttt tgtttgacac cgtcaaagag atatttgttg tccttgcggg ttaccttgag
     8521 aaaatatatt gttatcaaaa gctagctcgg ctagcttttg ttggaggatt ttttagaatg
     8581 aatgaatttg aagatttgct aaacagtgtt agcgaagtga accctggtga cgttgtcact
     8641 gcggaagttt taacagttga taacggtcaa gcaaacgttg ttatcgatgg tactggtgtt
     8701 gaaggtgtct tgacacttcg tgaattgact aacgatcgtg atgctgatat caatgatttt
     8761 gttaaagctg gcgacacagt tgaagtactt gttcttcgtc aagttgtagg taaagatact
     8821 gatacagtaa ctttccttgt atctaaaaaa cgcttggaag ctcgtaaagc atgggacaaa
     8881 cttgttggtc gtgaaggtga agttgttact gttaaaggta cacgtgctgt taaaggcgga
     8941 ctttcagttg aatttgaagg acttcgtggt ttcatcccag cttcaatgat tgatactcgt
     9001 tttgtacgta acactgaaaa atttgttgga caagaatttg atgctaaaat caaagaagtt
     9061 gatgcagcag aaaatcgctt catcctttca cgtcgtgaag ttgttgaaga atctgcagct
     9121 gcagcacgta aagaagtttt ctcaaacatt gaagtaggtt cagttgtaac tggtaaagtt
     9181 gctcgtttaa caagctttgg tgctttcatt gaccttggtg gtgttgatgg acttgtacac
     9241 gtaactgaat tatcacatga gcgcaatgtt tcacctaaat cagttgtaac tgttggtgaa
     9301 gaagttgaag ttaaagtact ttcaattgat gaagaagctg gtcgtgtatc actttcactt
     9361 aaagcaacta cacctggccc atgggatggc gttgaacaaa aacttgctgc tggtgatgtt
     9421 attgaaggta aagtaaaacg cttgactgac tttggtgctt ttgttgaagt gttacctggc
     9481 attgatggac tcgtacacat ttcacaaatt tcacacaaac gtgttgaaaa tcctaaagac
     9541 gtattatctg caggacaaga agttactgtt aaagttcttg aagttaattc agatgcagaa
     9601 cgtgtatcac tttcaatgaa agctcttgaa gagcgtccag cgcaagcaga aggtgaaaaa
     9661 gaagaaaaac gtcaatcacg tccacgtcgt ccacgtcgtc aagaaaaacg cgactacgag
     9721 cttccagaaa cacaaactgg attctctatg gctgacttat tcggtgatat tgaattataa
     9781 taattaaaag acttgcttta gcaagtcttt ttgtttttgt tgataaagta tgctataatg
     9841 taacatgtac taccatcttt agtgtaatgg atatcacaca agattccggt tcttgggata
     9901 ggggttcgat tcccttaaga tggataatag atgctccgta aagctttatg ttatagggtt
     9961 tatattagtt tatgccccaa ataattccct aataatttca ttgttcttgt tttttgtatc
    10021 ttccagcaaa tgagcatacg tgcttagtgt gatatttaaa tctttgtgtc caactatctt
    10081 ggaaatagca aacaagtcga ttccttctga tattaaatga gacacataag tatgtcttag
    10141 ggaatgaatg tgtatatttc tgccaactaa tttttttagt gtcttgttga cagctgtatt
    10201 tgatattcct tcaaatatcc ttgtttctgt tttgaataac aacctatatt gatctagcaa
    10261 tttgatagtt
//
LOCUS       DATRSD010000071        10227 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRSD010000071 DATRSD010000000
VERSION     DATRSD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.072"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            117..1400
                     /gene="aroA"
                     /locus_tag="VBD85_001566"
     CDS             117..1400
                     /gene="aroA"
                     /locus_tag="VBD85_001566"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO7134110.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1393..1905
                     /locus_tag="VBD85_001567"
     CDS             1393..1905
                     /locus_tag="VBD85_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO7134111.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1962..3335
                     /locus_tag="VBD85_001568"
     CDS             1962..3335
                     /locus_tag="VBD85_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7134112.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYRFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3436..4797
                     /gene="rlmD"
                     /locus_tag="VBD85_001569"
     CDS             3436..4797
                     /gene="rlmD"
                     /locus_tag="VBD85_001569"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7134113.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECIALIQR
                     VKS"
     gene            4794..4952
                     /locus_tag="VBD85_001570"
     CDS             4794..4952
                     /locus_tag="VBD85_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134114.1"
                     /translation="MKILHFKQFYKHYVFNEDGDGGRKKVLKNYIDVYVCIDMVCGDT
                     KGELGSEE"
     gene            4959..5240
                     /locus_tag="VBD85_001571"
     CDS             4959..5240
                     /locus_tag="VBD85_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134115.1"
                     /translation="MRWIIKIILFPISLLLSILTAFLTFILGIGTTILYFLMLMCIVA
                     AIGSFMQKDVSLGIEALIIGFLVSPYGIPMVGATVIAFLQGINEVIKSI"
     gene            5327..6106
                     /locus_tag="VBD85_001572"
     CDS             5327..6106
                     /locus_tag="VBD85_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO7134116.1"
                     /translation="MDFDYFYNREAERFNFLKVPEILVDGEEFKGLSAEAIILYSMLL
                     KRTGISFKNNWIDKENRVFIYFTVEEIMRRRNISKPTAIKTLDELDSKKGIGLIERVR
                     LGLGKPNIIYVKDFMSVFQAKGNDFQKSKNFTSEVKDIDLRSKENELQEVKNVDSNYI
                     ENNKSKYSKREYSFSENGLGTFQNVFLTDEDISDLQIKLNAQLDNYIERLSAYIKSTG
                     KIYKDHKATILSWFYKDQGKSEQAKTSNVPTWEEYNKGEHL"
     gene            6103..6954
                     /locus_tag="VBD85_001573"
     CDS             6103..6954
                     /locus_tag="VBD85_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7134117.1"
                     /translation="MKRELEKIMIEDVEFAYDSEKEYIKDGHAYCKVCHERKDGDVME
                     FFGNKMILRVACKCDREIEKQRKRREKQMEIERLKRTCFNSMREWSYTFENYQGEENK
                     SLIIAKNFVKDYEKMKKENIGLLFYGSVGSGKTYLACSIANSLIEQYQISVKIRNFAQ
                     IINELQKSSFDFDKNAYIESLVNTSVLILDDLGIERDTSYAKEQVYNIVNSRYLKQKP
                     TIFTTNLSYDTIQNCKDSVEYQRIYSRIIEMCIPIMVVGEDFRKFIQRDKLTRNKDRL
                     LNGGERT"
     gene            6951..7436
                     /locus_tag="VBD85_001574"
     CDS             6951..7436
                     /locus_tag="VBD85_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEO7134118.1"
                     /translation="MINEEIARKTLNMEVRAGKVTGKILLDLIRKLLKESEKIGGLEK
                     LVGSKGNEVKLKDMVKKGQLEEIPVEETELKELKKELNRYGVKFSVMKDKESGKYSVF
                     FQAKDMKVMDKAFKHALSESEKKTERKESIHKNIEKFKEMAKNSVSKDKIKNKQKEQS
                     L"
     gene            7433..9214
                     /locus_tag="VBD85_001575"
     CDS             7433..9214
                     /locus_tag="VBD85_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO7134119.1"
                     /translation="MIEKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHIRA
                     YTGGDVIDKIFQGILELNTMSFIPSIHPTDIIIGIGVAALIKFIVYSKGKNAKKFRQG
                     KEYGSARWGTRKDIEPYMDEKFQNNILLTQTERLTMNGRPKNPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNEYEIKILNTINFKKSMKYN
                     PFAYIRSEKDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDDETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMSEDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKFDITKHKNYKLLEDYDK
                     KNLFDIESYIKRKGKAKLNRETVITRV"
     gene            9292..9711
                     /locus_tag="VBD85_001576"
     CDS             9292..9711
                     /locus_tag="VBD85_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEO7134120.1"
                     /translation="MKEREKIIKLWFDMWLQQKDLGIDNIFTEDIVYTESWSPKYEDL
                     QTVKHWFNEWNTRGKVLAWDIKQFFHKDNQTIVEWYFKNKMNDGNIEEFDGISLVFWT
                     EDNRIKILKEFGCNCNNYNPYKDSEIPLFRNEKVNWF"
     gene            9746..10185
                     /locus_tag="VBD85_001577"
                     /pseudo
     CDS             9746..10185
                     /locus_tag="VBD85_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3767 a         1421 c         2077 g         2962 t
ORIGIN      
        1 aagttaattt ataaaagatg ttacagctat atagaggagc tatagtataa ataaaaaatt
       61 caaaatatag catatattat gatacaataa tagcaaaata gattttggga gttatcatga
      121 aattactgac taatgcaaat actctcaagg gaaccatccg tgttcctggc gataaatcaa
      181 taagtcacag ggctattatt tttgggagta tatctcaggg agttacccga attgttgatg
      241 tcttaagagg cgaggatgtt ttatcaacta tagaggcttt taaacaaatg ggggttctaa
      301 ttgaagacga tggcgagata atcactatct atggcaaagg atttgcaggt ttaacacaac
      361 cgaataatct cttagacatg ggaaactctg gtacttcaat gcgtctgata gcaggtgtgt
      421 tagctggtca agagtttgaa gtgacgatgg ttggagacaa tagtttgtca aagagaccta
      481 tggatcgtat agcattgcct ttaagcaaaa tgggagctag aatttcggga gtaactaacc
      541 gtgatttacc accattaaaa cttcaaggta caaaaaaatt aaaacctatt ttttaccatt
      601 tacccgtagc ttctgcacaa gtaaaatcag ctttaatttt tgcggcttta caaactaagg
      661 gagaatctct cattgtagaa aaagagcaaa caaggaatca tactgaggac atgattcggc
      721 aatttggtgg acatttggat attaaagata aagaaattcg actcaatggc ggtcagagct
      781 tagtaggaca agatataaga gttcctggtg atatttcgag tgctgccttt tggatagtag
      841 cgggtcttat tattcctaac agtcatatta ttttagaaaa cgtaggtata aacgagacac
      901 gaactggaat ccttgatgtc gtcagtaaaa tgggtggaaa aattaagtta tcttcggttg
      961 ataatcaagt aaaatcagca acccttacag ttgattactc tcatctacaa gctactcata
     1021 tttctggggc tatgattcca cgtctgattg atgaattacc tattattgct cttttagcaa
     1081 cacaagctca aggcaccaca gtcatagcag atgctcagga attaaaggtg aaagagacag
     1141 atcggattca ggtcgttgtc gaatcgctaa aacagatggg tgctgatatt actgctacag
     1201 cagatggtat gattatacgt ggtaataccc ctcttcacgc tgcttcactt gattgtcatg
     1261 gagatcatag aataggaatg atgatagcta ttgctgctct cttggtcaaa gaaggcgaag
     1321 tagatttatc tggtgaagaa gctattaata caagctatcc taacttttta gaacatttgg
     1381 agggattagt taatgcctaa ggtattattg ggattcatgg gagttggcaa gactagtgtt
     1441 gccaactgct tagaaaatga agtaattgat atggatagtc tgattgaaaa acatattggt
     1501 atgtcaattt cccgattttt tacagaagaa ggagaagcct cctttcgtgc tttagaatct
     1561 caatttttaa acgaactttt aaagaaaaaa aatgaaggac ttgtgatagc tagtggagga
     1621 ggtattgtac tccttgaaga aaatcgtcgt cttttaactt taaatcgtca taataatatt
     1681 cttttaacag gaagttttga agtcttatat catcgtataa aaaaagatga gaagaataga
     1741 agaccattat ttttaaacca ttcaaaagaa gaattttatg atatttatca aaaacgcatg
     1801 ttactctata gtggtttgtc tgatatgatt attgataccg attatcttac accacaaaaa
     1861 atagctactg taattggaga gtcactggtt aatttatcta actaagttat cacagtttgg
     1921 tagtatgaac tattattatt gaaagtaaat cacctgaaaa tatgtcaaga aataattacg
     1981 gtcagctaaa tcatcatgaa gagctacgat ataactatct tttaaaaaac atccattatc
     2041 ttaatgaacg tgaaaagatg gaatttcagt accttcatta taaaaaaaca gcagtacgac
     2101 cacagagaag gacagaaagt ccaccaacta attcttatta tgaggaacct tattctgatt
     2161 cttattatca agatgacgat ttttactcag agcctcagct aacctcacaa ggtttaccaa
     2221 tttatcaaga agagagagct ccgaagaaga aaaaacaaag ggccagaaaa gaaaaacaga
     2281 gggtgaaggt tatggcgcct ttccctccaa aggctataac cccccctaga aagaaaaaaa
     2341 aatttaaggg tttcctaaag ttcataggta ttatattact aattgtttta tcaggtatgg
     2401 ttttcatgtt tgttaaagga atgcgtgatg tgaataatgg gaagtcacat tatagccctg
     2461 caattattga agattttaag ggaaaagatg ctgttgatgg gactaatatc ttaattttag
     2521 gtagtgataa acgtgtcagc gagagatcta ctgatgcgag aacagatacc ataatggttg
     2581 ctaatgttgg taacaaggat aataaggtta agatggtgag ttttatgcga gatctcttag
     2641 tcaatattcc taattatagt acggaaggtt actatgatat gaaattaaat gcgtctttta
     2701 atttgggaga acaagataat cataaaggag cagagtatgt tcgacaaacg ttgaaaaatc
     2761 attttgatat tgatataaaa tattatgtta tggttgactt tgagacattt gcagatgcta
     2821 ttgatacact gtttcctaat ggtgtcaaga ttaatgctaa atttggactt gtcggtggac
     2881 aatctgctga ttcagttaaa gttcctgatg atttaaggat gaaaaatggc gttgtaccta
     2941 gccaaaaaat taaggttggg attcagtaca tggatggaag gacccttcta aattatgctc
     3001 gtttcagaaa agacgatgat ggggactttg gtcgaacaca acgtcaacaa caagttatga
     3061 gagctattgt ctctcaaata aaagacccac gtcgtctctt tacaggttct gctgctatcg
     3121 gtaaagccta tgctttaact tcaagtaatt tatcttatag atttgtccta acagatggca
     3181 ttccaatttt atcagatgct aaaaatggta ttaagcaaat gacaattcca agagaaggag
     3241 attgggtaga tgattatgac cagtatggtg gtcaagggtt aacaattgat tttgctaagt
     3301 acaaaaagat cctaaagaaa atgggactgc gttaacattt ttaattatga tataatagag
     3361 tgatttattg tcactctatt tttatcttta gtgtataaac taaagacttc aaatgataga
     3421 tataaaagga aaattatgtt acataaaaat gatattattg aaactgaaat tagcgacatt
     3481 agccatgagg gaatgggtat tgccaaagta gatggctttg ttttctttgt tgaaaatgct
     3541 cttccaggtg agattattaa gatgcgcgtc ttgaaattac gtaagcgtat aggatatgga
     3601 aaagttgagg aatatttaac gacttctcct caccgaaatg aaggattaga ctatacctat
     3661 ttacgtacag ggattgctga tttgggacat cttacttatg aacaacagct gttatttaaa
     3721 caaaaacaag tagctgataa cctttataaa attgctcata tatctgatgt tttagtagaa
     3781 cctactcttg gtatgacaat ccctttggca tatcgaaata aagctcaggt accagttcgt
     3841 cgtgttgatg gacagctaga gacaggtttt ttcagaaaaa attcacatac tctggtatcc
     3901 attgaagatt atttaattca ggaaaaagag attgatgctc tgattaattt tacacgagat
     3961 ttattaagaa aatttgatgt taaaccatat gacgaagagc agcaatctgg tttaattaga
     4021 aatctcgttg ttcgtcgagg acattataca gggcaattaa tgttggtttt ggtgacaaca
     4081 cgtcctaaaa tattccgcat tgaccaaatg attgaaaagt tggtgtctgc ctttccttct
     4141 gttgtttcaa ttatgcaaaa tattaatgat cgtaatagca acgttatttt tgggaaagaa
     4201 tttcgtactc tatatggttc tgatactatt gaggaccaaa tgttaggaaa tacttacgct
     4261 atatcagccc aatcttttta ccaagttaat actgaaatgg ctgaaaaact gtaccaaaaa
     4321 gcaatcgatt tttcagattt aaactcagag gatatcgtta ttgatgccta ttcaggtata
     4381 ggaacaattg gcttatctgt tgctaaacaa gtaaaacatg tttatggtgt tgaagttgtt
     4441 gaaaaagcag taagtgatgc caaagaaaat gctactcgaa atgggataac taacagtacc
     4501 tatgttgctg attcggctga aaatgcaatg gcaaagtggc ttaaagaagg tataaaacca
     4561 actgttataa tggttgatcc accacgaaaa ggattaacgg agagttttgt atattctgct
     4621 gcccaaacaa aagcagataa aatcacttat atttcttgta attcagcgac aatggcgcgt
     4681 gatatcaaac tatttgaaga attagggtat catttagtaa aaattcaacc agtagactta
     4741 ttcccaatga cacatcatgt ggagtgcata gcgttgatac aaagagtgaa atcgtgaaaa
     4801 tcctgcattt taagcagttt tacaagcatt atgtctttaa tgaagatggc gatggaggaa
     4861 gaaaaaaagt cctaaaaaat tatattgatg tttatgtttg cattgatatg gtgtgtggag
     4921 atacaaaggg tgaattggga agtgaggaat aagaaatgat gagatggata ataaaaataa
     4981 tcttgtttcc aatcagcttg ctgctgagta tccttactgc ctttttgaca tttatacttg
     5041 gcataggaac aactattctg tatttcttaa tgcttatgtg tatagttgct gcaataggct
     5101 cgtttatgca gaaagatgta tcacttggta ttgaagcatt gattattgga ttcttagtta
     5161 gtccatatgg aataccaatg gttggagcaa cagttatagc ttttttacaa ggtatcaatg
     5221 aggtaataaa atcaatctaa aaaatttcat aaaaatggtt accaaaggcg atagaagaaa
     5281 aactatcgtc tttttctttg caaattttaa gagaggaggt gaagcgatgg actttgacta
     5341 tttctataat cgtgaagcag agagatttaa ttttcttaaa gtaccggaga tattagttga
     5401 tggagaagaa tttaaggggt tgtcagcaga ggcaattatc ctttattcca tgcttctaaa
     5461 acgaacggga atatctttta agaataactg gatagacaaa gaaaatagag tatttatcta
     5521 ttttaccgtt gaagaaatta tgagaagaag aaatatctca aagccgacag ccataaaaac
     5581 attggatgag ttagacagta aaaaaggaat cggactgatt gaaagagtaa ggcttggact
     5641 tggaaaacct aatatcattt atgtaaaaga ctttatgagt gtatttcaag caaaaggaaa
     5701 tgactttcag aagtcaaaaa actttacttc agaagtaaaa gatattgacc tcagaagtaa
     5761 agaaaatgaa cttcaggaag ttaaaaatgt tgactctaac tatatagaga ataataagag
     5821 taagtatagt aagagagaat atagttttag cgaaaacgga cttggaacat ttcaaaatgt
     5881 atttttaact gatgaagata tttctgattt acaaatcaaa ctaaatgcac agcttgataa
     5941 ttacattgaa cgcttatctg catatatcaa gagtaccgga aagatctata aagaccataa
     6001 agcgacgatc ctttcttggt tttataaaga tcagggcaaa agcgagcagg caaaaacatc
     6061 aaatgtcccg acatgggaag aatataacaa aggagaacat ttatgaaaag agaattggaa
     6121 aaaataatga tagaggatgt agaatttgcc tatgattctg aaaaagagta tatcaaagac
     6181 ggacacgcct attgtaaagt ctgccacgaa agaaaagacg gtgatgtaat ggaatttttc
     6241 ggaaacaaaa tgatattaag ggtagcttgt aaatgcgata gagaaataga gaaacaaagg
     6301 aaacgaagag aaaaacagat ggaaattgaa aggttaaaaa gaacctgctt caattccatg
     6361 agggagtggt catatacctt tgaaaattat cagggagaag aaaataaaag cctgatcatt
     6421 gctaaaaact ttgtcaaaga ttatgagaaa atgaaaaaag aaaatatcgg acttctcttt
     6481 tatggctctg taggaagcgg aaagacctat cttgcctgct ccattgcaaa tagccttatt
     6541 gaacagtatc aaataagtgt taagattaga aatttcgcac agattatcaa tgaacttcaa
     6601 aaaagcagct ttgactttga taaaaacgca tatattgaat cccttgtaaa tacttccgtt
     6661 cttatcttag atgacttggg aattgaaagg gatacaagct atgctaaaga gcaggtatat
     6721 aacattgtca atagtaggta cctaaaacag aaaccaacaa ttttcacaac aaatctttcc
     6781 tacgacacaa ttcaaaactg caaggacagt gttgaatatc aaagaatcta ttcaagaatt
     6841 attgaaatgt gtattcccat tatggttgta ggcgaggatt tcagaaagtt tattcaaaga
     6901 gataagctaa ctcgcaataa agatagatta ctgaacggag gtgaaaggac ttgatcaatg
     6961 aagaaattgc aagaaaaacg ctcaacatgg aagtgagagc cggcaaagta acaggcaaga
     7021 tacttttgga tttgataagg aaattactga aagaatcaga aaaaatcggt ggacttgaaa
     7081 agctcgttgg gagtaaaggg aatgaagtaa aactaaaaga tatggttaaa aaaggacagc
     7141 ttgaagagat accagtagaa gaaacagaat tgaaggaact aaaaaaagaa ctgaacagat
     7201 atggagttaa gttttcggta atgaaagata aagaatcagg taaatactca gtgttctttc
     7261 aagcgaaaga tatgaaagtg atggacaaag catttaagca cgccctttca gaatcagaaa
     7321 agaaaacgga aaggaaagaa tctattcata aaaacattga gaagtttaag gagatggcta
     7381 aaaattctgt ttctaaagat aaaatcaaga acaagcaaaa ggagcagagc ctatgataga
     7441 gaaaatacta aaggacatca aaggcttgtt taaggtgcag gataaggcaa agtttctaaa
     7501 gcagaacatt ccctatcttg catttttcta tgttggaaat atcttctctc accatatacg
     7561 agcctatact ggtggcgatg taatagacaa aatctttcaa gggatattag agcttaacac
     7621 catgagcttt attccaagca ttcatccaac tgacattata ataggcatag gagtagcagc
     7681 tttaattaaa ttcattgtct acagcaaagg caaaaatgca aaaaagttca gacagggaaa
     7741 ggagtatggt tcagcaagat ggggaacgag aaaagatata gaaccataca tggatgaaaa
     7801 attccagaac aatatcttgc ttacacaaac agaacgatta actatgaatg gtagacctaa
     7861 aaatcctaaa tatgctcgta acaagaatgt acttgttata ggaggtagtg gttctgggaa
     7921 aactcgattt tatgtaaaac cgaacttaat gcaaatgcac tcgtcatatt gcgtgaccga
     7981 tcctaaagga acaatagttc ttgagtgtgg caagatgctt gaagataatg aatatgagat
     8041 taaaattcta aataccataa acttcaaaaa gtctatgaag tacaatccat tcgcatatat
     8101 ccgttctgag aaagatattt taaaactcgt tcagacaatc atcgcaaaca cgaagggcga
     8161 gggagaaaaa gcaggtgagg acttttgggt gaaagctgaa aaactctact atacagcact
     8221 tatcggatat atcttttatg aagctccaag agaagaaaag aactttgcaa cactacttga
     8281 tatgatagac gcttcagagg taagagaaga tgatgaaact tacatgaatc cgattgatag
     8341 gctctttgaa gcgttagaaa agaaagaacc aacacacttt gcggtgaagc aatataaaaa
     8401 gtacaaattg gctgcgggga aaactgccaa gtctattcta atatcttgtg gagcaaggct
     8461 tgctccattt gatatacagg agcttaggga cttgatgagt gaagatgaac ttgagcttga
     8521 tacactggga gatagaaaga cggcactctt tgttattatc tcggatacag atgatacttt
     8581 taactttgtg gtgtctatta tgtactcaca gctgtttaat ctgctatgtg ataaggctga
     8641 tgatgtgtat ggaggaagat tacctgttca tgtaagatgc ctacttgatg agtttgcaaa
     8701 catcggctta attccaaagt ttgaaaagct aatagcgaca atccgttcca gagaaatatc
     8761 agcaagtata attcttcaag cacaatctca gctaaaggca atctataaag ataatgctga
     8821 cacaattgtg ggcaactgtg atagtacctt gtttcttggt ggcaaggaaa agaccacatt
     8881 aaaggaactt tccgaaacac tgggaaaaga aacgatagat ttatataaca catcggaaac
     8941 aaggagcaat caaaagagtt ttggacttaa ttatcaaaag acaggtaagg agcttatgag
     9001 ccaagatgaa attacagtca tggacggtgg taaatgtatt tttcagcttc gtggtgtcag
     9061 accttttcta tcggataaat tcgatattac aaagcataag aactataagc tgcttgagga
     9121 ctatgacaag aaaaatttgt ttgacataga aagctatatt aagcgaaaag gaaaagcaaa
     9181 gctgaataga gaaacagtta ttacaagagt atagaaatct gtgattgttt agatatacct
     9241 gcatttgata taataagtta agaaatatct ttaaaaaggg gttgatatgc tatgaaagaa
     9301 agggagaaaa tcattaaatt atggtttgat atgtggttac aacaaaaaga tttgggaata
     9361 gataatattt ttactgaaga tattgtttat actgaaagtt ggagtcctaa atacgaagat
     9421 ttgcagacag tcaagcattg gttcaatgaa tggaatacaa gagggaaagt tcttgcatgg
     9481 gatattaaac aattttttca taaggacaat cagactattg tggagtggta tttcaaaaat
     9541 aaaatgaatg atggaaatat tgaagaattt gatgggattt ctttggtttt ttggactgaa
     9601 gataatagga taaaaatcct aaaagaattt ggctgtaatt gtaataacta taatccttat
     9661 aaagatagtg aaatcccatt gtttagaaat gaaaaagtaa attggtttta aaatgaaaaa
     9721 aactgagatt aaggaggaaa tgttattgaa tgataaagag ctgataggaa aagttcattc
     9781 atcaatgtat cagcaattaa aaagaaaagg ctatgcaacg gtaatagatg tgcttatgga
     9841 tttaggtata ctttcaaaag aaaattatga actttggaga aatgggaaag tttcatattt
     9901 agaaaaagta tgcgatatca agctaaaaaa gctctccaca atcttacatg aaataagagt
     9961 atatgcgaag aaaatgagct tgaatccatc ctattgcgta tataaaaatg ggcggtaaag
    10021 aaaaagaacg gacaatggaa aaagccggta ataaagctaa ggtttattaa aagcagttca
    10081 gatgatattg agagatggta tgccactcat tttgtagata caaaaagaat agaacagctt
    10141 aaagaagaaa aacaaaaaat agttaataat gatgaaaagc agtaagtcta aaaaatagat
    10201 ttgctgcttt tttcgtaccc ggaaaat
//
LOCUS       DATRSD010000072         9649 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRSD010000072 DATRSD010000000
VERSION     DATRSD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.073"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(286..483)
                     /locus_tag="VBD85_001578"
     CDS             complement(286..483)
                     /locus_tag="VBD85_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134121.1"
                     /translation="MQIKTFNMAYPDKSFKPTPINELIEKFDKEINQFMENQKEVISV
                     NTQTNGLAGGFQYFVTVVYRN"
     gene            complement(594..971)
                     /locus_tag="VBD85_001579"
     CDS             complement(594..971)
                     /locus_tag="VBD85_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO7134122.1"
                     /translation="MNKKNAMRKLKEFQRWQRIANSLDLTYNELYQFDIEYHSTRRTH
                     LEIRRECALEELGAIKYAINQLSKIDYRQILIECYLISEKLSNQEIMTKLNRSESWYY
                     ETKKRALLEFAKYYRDGILNKIN"
     gene            complement(946..1308)
                     /locus_tag="VBD85_001580"
     CDS             complement(946..1308)
                     /locus_tag="VBD85_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO7134123.1"
                     /translation="MGAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAQQLLCLAKERDKRLAMITSLQDEKQIAVIKARYVDDLSWDEIPDKVGCSRNTVF
                     KLHRDALEVLDEQEERHA"
     gene            complement(1706..2194)
                     /locus_tag="VBD85_001581"
     CDS             complement(1706..2194)
                     /locus_tag="VBD85_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134124.1"
                     /translation="MKELSIESIIKPMKKYDKTKITGTMDNQPIRIDLDNLVIHYNHQ
                     NLLLETIPGTYGGKRYFFLCPKCERRCRKLFKIYNIFACGSCQKVHQATLNRSKTDCQ
                     YYWQLAFKECLKVDPKARHKHDYYSREDFPKRPKYMRLSKYLYHWRRFHYYMDKGASH
                     WL"
     gene            complement(2279..2884)
                     /locus_tag="VBD85_001582"
     CDS             complement(2279..2884)
                     /locus_tag="VBD85_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134125.1"
                     /translation="MRTFSDTPKTFTFRYTFRDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYAEDRKLSKVFKRICDSFKDYYNQPADMTDEELDDMDQEN
                     ELIKEVKMCNDNQLFTKEYIAKRTTQLEDIDKTILIRDIIAYELELLDYADRLLSDKS
                     IRMDSETAQGTIELMDDNVINLVKELDTEREYQGLHNYVIS"
     gene            complement(3068..3241)
                     /locus_tag="VBD85_001583"
     CDS             complement(3068..3241)
                     /locus_tag="VBD85_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO7134126.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNLFTLSVSVIDIKFSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            complement(3501..5003)
                     /locus_tag="VBD85_001584"
     CDS             complement(3501..5003)
                     /locus_tag="VBD85_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEO7134127.1"
                     /translation="MKSLESEILEARENEQPPKTMRELENRIFQAGEQWREEHTETKI
                     NESTGDVTEKVAIPQVFTVAKMLSEIITFTFISKSNVPDYSLLYIYDLDEGIYTASND
                     LFNRFCKTFDVRIKPREWPQIKLMVRTLTRIKKPLESAYFIPVQNGIIDLRTKELLPF
                     SPKYVITSKISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPN
                     HTRNKFAIFYGDGNNGKGTFQRFLINLIGESNVSALKPVQFAEKHNLETLVGKVCNIG
                     DEAPNEYLKNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGW
                     YRRIMIVPFNADFNGEKEKPWIKNDFLGKKKVLEYALYKAINQESFTHFIEPQAVKGL
                     LEEYQEDNDYLLSWVKHEYMERGWHELDVVPVFIATRSLKHYAEDMGIPKPNVYGAGK
                     ETIRHLQQLTQNKYQLKKARVKVEDLDKLDPLEFERPKLGRVNHAITKKE"
     gene            complement(5023..5880)
                     /locus_tag="VBD85_001585"
     CDS             complement(5023..5880)
                     /locus_tag="VBD85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEO7134128.1"
                     /translation="MAIYETRGFSSYLYPYKGQLEPFDYIAHFKPLKPPEGIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIEIGVNLPKIVSETLWEYS
                     YIIYPTIKHTPKKPRYRLVVKPSDVMNEEIYKQVVKDIADKIGLPFDLASLTWSQLQG
                     LPITTGVPEDYQHYANRGLDYPVPKNGSTPNQQVVTTYTPRSRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDIETAYELTKIANSVTANPLPERELDRTFESI
                     ARAEFRKRG"
     gene            complement(5881..6153)
                     /locus_tag="VBD85_001586"
     CDS             complement(5881..6153)
                     /locus_tag="VBD85_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7134129.1"
                     /translation="MFSLSKESEQDLTQGVLELVGNYLEVREQTQPRLLGLITAQQIK
                     DELGIKAKTLKRWEDNGLRRYQPPLEDTRKHYYKVSDILIFLGVDT"
     gene            complement(6146..6487)
                     /locus_tag="VBD85_001587"
     CDS             complement(6146..6487)
                     /locus_tag="VBD85_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134130.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSIQSIISS
                     LITRYNVPIVAIRHGFNRGYFIPANKEELLEGAKAFYNQVQKEQERLSVLMNADLESY
                     KKLLKEADMNV"
     gene            complement(6484..6846)
                     /locus_tag="VBD85_001588"
     CDS             complement(6484..6846)
                     /locus_tag="VBD85_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134131.1"
                     /translation="MTTEEYFKKLLEDSEKHPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRDYNKADAVVKFLESMPIEMEYKKGLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            complement(6858..7055)
                     /locus_tag="VBD85_001589"
     CDS             complement(6858..7055)
                     /locus_tag="VBD85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134132.1"
                     /translation="MAIQELNFTPTQTLILFIVLGLLGFLLSRSKSLIDIDLPEDTQA
                     PKPPQNANYGAYIQSQNHYYN"
     gene            complement(7036..7176)
                     /locus_tag="VBD85_001590"
     CDS             complement(7036..7176)
                     /locus_tag="VBD85_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134133.1"
                     /translation="MRSIEELKAIAEYILEHMTDSEKAHFPNMTREEKKGLIIKYGNS
                     RT"
     gene            complement(7173..7505)
                     /locus_tag="VBD85_001591"
     CDS             complement(7173..7505)
                     /locus_tag="VBD85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134134.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEAYFTRLYNQH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            complement(7760..8002)
                     /locus_tag="VBD85_001592"
     CDS             complement(7760..8002)
                     /locus_tag="VBD85_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEO7134135.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPNADREQFEAITQE
                     IFNVLEGVSLMLDDKALIYNRKAEKKRLNDIEQGNY"
     gene            complement(8029..8649)
                     /locus_tag="VBD85_001593"
     CDS             complement(8029..8649)
                     /locus_tag="VBD85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO7134136.1"
                     /translation="MKTEHWNGYTIRFVEHQNEWWAVLADIAKALDLNPKFIKQRLGD
                     EVVSNNHVADSLGRQQEMLIVNEFGIYETIFSSRKKEAKTFKLWVFEIIKQLRQSTGL
                     EGFQVFRIFDKEHQKQAMNRLVDGLQNATKKDLIKANTIANKAVSDLYGYPKMISKNE
                     MTENMLRDREPILDETVELIKVKEKYGLNFSVSEAIYNQNTIKKAQ"
     gene            complement(8703..8864)
                     /locus_tag="VBD85_001594"
     CDS             complement(8703..8864)
                     /locus_tag="VBD85_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134137.1"
                     /translation="MVKNNLHIVMALNNTKASEVAEKTGISKSTISKFINGKTDIKLS
                     TLISLCIFF"
     gene            9030..>9649
                     /locus_tag="VBD85_001595"
     CDS             9030..>9649
                     /locus_tag="VBD85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134138.1"
                     /translation="MLINRLAILLAERSLSGSRLAVDTGIAQSTISKITSNKSKQVDY
                     ATVNTICNNLKVTPSDFFEYSPIDYDISCVKDEDDSSILIFIKVSKFDTKICTLEFKA
                     VPKYYVNQDQPDFFEESSLSIDKIKKEYTKVDKLIISLEAKNINKEKCDDFYDLPIVF
                     QNILKNDLMEKVGYFLKNEIINVFSDEFTFKKNSFGRVDLLYFNSKA"
BASE COUNT         2703 a         1849 c         1607 g         3490 t
ORIGIN      
        1 aaatttagaa gtgctatttc tcgtaacatt atatattctg aaaggcttat ctgattatca
       61 gtacaaaatt gtctcctgct tatcctattt ttgctaggtt tttagtattg attggtcaag
      121 atagcttttt cagtgttttc tagctcgtta accttgtttc ctatcaactg ggcaattcca
      181 ccatgtaacg aaaaaggttt atcagatagc ttatatagac gtttaaatac agcatagttt
      241 ttaaatattc tatccgctta tttaatttct tttgtccctt caaagtcagt ttcgataaac
      301 tacagtgaca aaatattgaa aaccaccagc gagtccattt gtttgtgtgt taacagagat
      361 aacttctttt tgattttcca tgaattgatt gatttcttta tcaaactttt ctatcaattc
      421 attaatgggt gttggtttaa acgacttatc tggatatgcc atattaaaag tttttatttg
      481 catttcacta cctccattta aaaaataaat ttaaattatg aagatattat atcaaaaaat
      541 tactccctgg cggatattat gccacatata agatataaag gtactatgat tcattaattt
      601 attttgttta gaattccatc cctataatac ttcgcaaact caagcaaagc tcttttctta
      661 gtctcgtagt accaactttc agagcgatta agtttcgtca taatctcttg attagataac
      721 ttttcactaa tcaagtaaca ctcaatcagt atttgtctgt agtctatctt agacagttgg
      781 ttaatggcat acttaattgc acctagttcc tctagggcgc attctcgcct tatttcaagg
      841 tgcgttctgc gcgtggaatg gtattctata tcaaactggt aaagctcgtt ataagttaaa
      901 tcaaggctat tggcgatacg ttgccacctc tggaactcct taagtttacg catggcgttc
      961 ttcttgttca tctaacacct ctaaagcgtc tctatgtagt ttgaaaacag tattccttga
     1021 acaacctact ttatctggta tctcgtccca tgataagtca tccacatatc tagccttaat
     1081 aacagctatc tgcttctcat cttgtaggga ggtaatcatg gctagcctct tgtcacgttc
     1141 ttttgccaaa cataagagtt gctgtgcagt acctttttca atgccattta gtaactcagg
     1201 attacgaaaa gcattcatca gcttgatgtc cttgtctcgt tgttcctcaa ataaggtcat
     1261 taaagcaaaa agtggcttca attcttttaa ttgttcttta gcgcccatga ttaactcctt
     1321 tttggtataa ttcagttaag cttaaattta actaaggaga cgttctgtgt gagcgtcttt
     1381 ttgtgtttct cgtttcgtaa actagatatt ttttgtagta cttcgctttt ggcttctaaa
     1441 atcgtttcta agcgcttttt aaagttggta atataaaata tcaacttgtc agatagaagg
     1501 tctaaaacca tgctttattt tagtctgata aagtcattct ttgtggtagg gtacttagct
     1561 taaattagat ataaaatcct ttagaatcaa gcttttaact tttatcaaaa atttcaaaaa
     1621 gggaattttt tgcacggaaa aaggcgcgtt ctcaaaaaca cgaacgatat agccccgtta
     1681 aaaaaagagt gggggtgttt ccgtattata accagtgact ggctccctta tccatataat
     1741 aatgaaatct cctccagtga tataagtatt ttgacaatct catgtatttt ggacgcttag
     1801 gaaagtcctc acgactataa taatcatgtt tatgtcttgc ttttggatct actttcaaac
     1861 actctttaaa ggctaattgc caatagtatt gacaatctgt cttgcttcgg ttgagtgtcg
     1921 cttgatgaac tttctgacaa gaaccacagg caaagatatt gtaaatttta aacagtttcc
     1981 gacaacgtct ctcacattta ggacacaaga agaagtagcg tttaccacca taagttcctg
     2041 gtatcgtttc aagtaataag ttttgatggt tataatgaat aaccagatta tctaggtcta
     2101 tgcgaatagg ttggttatcc attgttcctg taatctttgt cttgtcatat ttcttcatcg
     2161 gtttaataat actctcaatg gataattctt tcatactgtc tcctaaaggt tcaaacaacc
     2221 caaaattatt taataaatga caaaaagagg gaaatccctc tttgttaccg tgcgctactt
     2281 agctaatgac ataattatgt aacccttggt actcgcgttc ggtatccagt tcttttacta
     2341 aatttattac attatcatcc ataagttcaa tagtcccttg tgcggtttca ctatccattc
     2401 gaatagactt atcgctcaat agtctatcag cgtaatctaa caactctaat tcataagcaa
     2461 tgatatccct aattaatata gttttatcaa tatcctctag ttgtgtagta cgcttagcaa
     2521 tgtattcctt tgtaaataac tgattatcat tacacatttt cacttccttg attaattcgt
     2581 tttcttgatc catgtcatca agttcttcat ccgtcatatc cgcaggttgg ttgtaatagt
     2641 ccttgaaact gtcacagata cgcttgaaga ccttgcttaa ctttctatct tcagcatatt
     2701 ttaagactaa ctgattagca tgaccacctt ggtcatcatt gtgataagtt gcgtcaatca
     2761 ctggttgctc ataagtacca gtcatgtaac ctaagatagc atgacaagct acttgtgcag
     2821 tgtcaaagtc tctaaaagta taacggaatg taaatgtttt aggtgtatct gaaaatgttc
     2881 tcatagtgtt atttctcctt tgtgattgct ataatttctg ataaattgat ggtgccagaa
     2941 ggagtcttta cccaggtagg tcgtcggcca gataggagaa tctcaaccaa ttcatcataa
     3001 agggtgcggt ctccttgtac ggtgatggtg ttaccacctc gtgtgtgtag ttttagtttc
     3061 atactagtta cctgtacaag accaataaag aagttgtagt actcatatcc tcataattgc
     3121 cataagttgc ttctgaaaat ttgatatcta ttacagatac tgatagagta aagagattga
     3181 cccgatattc aaaatcatct agtgattcat tgtgtttttg ataaaatagt ttgattttca
     3241 ttttttagct cctttttttc aatttgttac cttctatagt gattttgtta ccttctaggt
     3301 aacatagctc cgccttactg ccacaagggt ctagccgctt tgttaccatg ttgaccttct
     3361 ttctaactcc ttacccttat atatataaat actgcttttt ttcctatata gagagttaaa
     3421 aagaaggtaa caagaaggtc aacaaatttt gtaaaatcct agtaactgca agggtttaga
     3481 tcatcaaaag aaggtcaaca ttattctttt tttgttatag cgtgatttac tctccccagt
     3541 ttcggtctct caaactccaa agggtctaac ttgtcaagat cttcaacctt tacacgcgct
     3601 tttttgagtt ggtacttatt ttgtgttagc tgttgtaagt gcctgattgt ctccttacca
     3661 gcaccgtaaa catttggctt aggtattccc atatcttcag cataatgctt caaggatctt
     3721 gtagcgataa aaacggggac tacgtccagc tcgtgccaac ctctttccat gtactcatgt
     3781 tttacccaag ataacaagta atcattatct tcctggtatt cctctaacag gcctttgact
     3841 gcctgcggtt cgataaaatg agtaaacgac tcctggttga tagctttata aagggcatac
     3901 tctagcacct tttttttgcc taaaaaatca tttttgatcc agggcttttc tttctcaccg
     3961 ttaaagtcag cattaaatgg gacaatcata atacgcctat accagccctt tgtcttattt
     4021 ccaccgttgg gaatatagtt tcctgaaaag atattaaaga gtttgaaggt cgcttcaaaa
     4081 gctgggcgcc cctttggatt gactagcacg gtgtccccgc tggtaatact cattaggtca
     4141 gacggatttt ttaagtattc attaggtgcc tcgtctccaa tattgcaaac tttacctact
     4201 agcgtttcca ggttatgctt ttcagcaaac tggacgggct tcaatgccga tacgttactt
     4261 tctcctatca gattgataag gaaccgctga aacgtccctt ttccgttgtt accgtccccg
     4321 taaaagatag caaacttatt ccgtgtatgg tttggattga tagcctccag gataatctgc
     4381 caaaacaagg ttactagttc actatcattg caagcgattg agtttaacca atcgtcaaat
     4441 gtcttccctt ccctatcggt tgggacccgc ttaggcgcgt ggtaagctgt actgatttta
     4501 cttgtaatca catatttagg actgaaagga agtagctcct tagtccttaa gtcaataatg
     4561 ccattctgta cagggataaa gtaggcgctc tccagcggtt tctttatcct tgtcaatgtc
     4621 ctaaccatta gcttaatctg gggccattcc ctaggcttaa tcctcacgtc aaaagtctta
     4681 caaaatcggt taaataggtc attactagcc gtatatatgc cctcatctaa atcatagata
     4741 tagagtaggc tataatcagg tacgttgctt ttactgataa aagtaaaggt gataatttcg
     4801 cttagcattt tggcaactgt gaaaacctgg ggtatggcca ccttttcggt aacgtcccct
     4861 gtactttcat ttattttggt ttccgtgtgt tcttcccgcc attgttcacc agcttgaaag
     4921 atacggtttt ctagctctct catggtcttg ggcggttgct cattctcacg cgcctctaag
     4981 atttcacttt ccaggctttt caattcttcc ttttctatga ttctatcctc tctttctaaa
     5041 ttctgctctt gctatacttt caaaagtcct atctagctcc ctctctggta gggggttagc
     5101 tgtcacactg ttagcgatct ttgttaattc gtaagctgtt tctatatcac aatccaccca
     5161 tttattaaat agcaagccaa caaacttagt taaggccacg ttgcgcccgc cttcgtctcc
     5221 aaaaccatta aacaaggtat ctattaccct catagtgata gaccgctgac ttctagagcg
     5281 tggcgtgtaa gtagtaacaa cttgttggtt tggcgtgcta ccatttttag gaacaggata
     5341 atcaagacca cggttcgcat agtgctgata atcttctggg acgcctgttg tgatgggtaa
     5401 gccttgtaat tgcgaccagg taagactagc taaatcaaaa ggtagtccaa tcttatcagc
     5461 tatgtcctta actacctgct tataaatttc ctcgttcatc acgtcactag gcttcacaac
     5521 aagacgataa cggggcttct tgggtgtgtg tttaatcgtt ggataaataa tataactgta
     5581 ctcccaaagt gtctcagaaa cgattttagg taggtttacg cctatttcta tttcatcata
     5641 gtcaagaaaa atcaaatcac ggtaaaccaa actagcatta ttacgcttat agctaccgtt
     5701 tttctctgct gtgactttgc cactcaggca gtagggagct tgtgttcgct tgtattcttc
     5761 aatatcgatg ccttcaggag gtttcaaagg tttaaaatga gcaatatagt caaatggttc
     5821 taactgtcct ttgtaggggt agagataaga actaaaacct cttgtttcat aaatagccat
     5881 ttacgtatcc acccccaaaa aaataagaat atcactgact ttatagtaat gcttcctcgt
     5941 atcttctagt ggtggttggt atcgtcttag accgttatct tcccaacgct ttaatgtctt
     6001 ggcctttatg cctagttcat ctttaatttg ctgggctgtt atcaacccta atagtctggg
     6061 ctgggtttgc tcacgtactt ccaggtaatt tcctaccagc tccagtaccc cctgagttaa
     6121 atcctgttca ctctctttac ttaaactaaa cattcatatc agcctccttc agtaacttct
     6181 tataactctc taggtccgca ttcattaata cagacaaacg ttcttgttct ttttgtactt
     6241 ggttataaaa ggctttggca ccctctagta attcttcctt attagctggg atgaagtagc
     6301 cacgattaaa gccatgtctt atagcgacaa tagggacgtt atagcgcgtg attaagctac
     6361 tgatgatact ttggattgaa cgttcttcaa gtttcagtat taagctaatc tctgcccctg
     6421 tgataggatt gtctgctcca accttgatca gattaaggac acgtctataa ttctctggta
     6481 gtgtcatttc gtgtcctcct tatcatttgg aaacaggatt tggttatcaa taacaccaag
     6541 cacttgattt ttaagactat cttttaactc gttcaatgct tgtataacta tatttagccc
     6601 ttttttatat tccatctcta ttggcattga ttccaaaaat ttaacaacgg catcagcttt
     6661 gttatagtca cgttgtgtag tattgataaa tatttttttc gtgaataacg gaaaatcgtc
     6721 ccactctgct tcaatatctt catctagcat tttgatattt ttgtctaaaa ccctatcact
     6781 caaccagttt gtaggatgtt tttcgctatc ttctagcaat tttttaaaat attcttccgt
     6841 tgtcatgcag ttcctctcta attgtaataa tggttctgtg attgaatata agccccatag
     6901 tttgcattct gaggtggttt aggtgcttgg gtatcttctg gtaaatcaat gtctattaaa
     6961 gacttagaac ggctaaggag aaaccctaag agacctaaaa caatgaataa aataagtgtc
     7021 tgtgttggtg taaagttaag ttcttgaatt gccatattta attatcaatc cttttttttc
     7081 ttctcttgtc atgttaggaa aatgggcttt ttcactatcc gtcatgtgtt ccaaaatata
     7141 ttcagcaatc gcttttaatt cttcaatact cctcatgcgg atacctcact taaataagtt
     7201 tctaattccc ctgtgtcttt ctctgaacat ggtaaaccat taacggctct aaagacaatc
     7261 tctgtggttt gtttataacc taaagcgccc catgcttctt caaaagtagt ggcacttttc
     7321 ctgaatgtag tcgtatactc tgccattaca ttagcaataa tcacccgagc aatatgttgg
     7381 ttatatagtc gagtgaaata tgcttcagct ttatctttgc tgagttggcg atttttgaac
     7441 atttctagct gttcaggagt gtatctatct tttgaaaaag gatttgtttc tactctatat
     7501 ttcattatgt cttttctcgc ttaattttat tttctgtgta gtattcttat tgattgcttg
     7561 tttcttgtac tagattcgtg ctagttttaa aggatagtag tcaaaatatc ctagatatgg
     7621 tataatctag ctatcaaatc ttttactaaa acctctttaa taatagcttg cctgctttat
     7681 tatttgagtt tagttatact agttaaaggc tgtgctgatt ggtctcggta agcctttttt
     7741 tgttgctctt gattattgat taataattgc cttgttcaat gtcattcaaa cgctttttct
     7801 ctgctttgcg attatagatt agagctttgt catcaagcat gagcgatacg ccttccaata
     7861 cgttgaaaat ttcctgtgtg attgcttcaa actgttcgcg atctgcattt ggtactttgt
     7921 cagcgtaacc ctgtgctagc tcagctaaat caacaccttc atcaatccat ttcttcaact
     7981 ctttgtaagt tgttgctttc ataatcattt ctcctttatc cacgcgcatc actgcgcttt
     8041 ttttattgtg ttttggttat agatagcttc agatacacta aaatttaagc cgtatttttc
     8101 tttaaccttg attagttcaa ccgtttcatc aaggataggc tcacggtctc gcaacatgtt
     8161 ttctgtcatc tcgtttttac taatcatctt aggataacca tataggtcag aaacggcttt
     8221 gtttgcaata gtatttgctt tgataaggtc tttcttagtt gcattttgta agccatcgac
     8281 aagcctattc attgcctgct tctgatgttc tttatcaaat attctaaata cttggaagcc
     8341 ctctagccct gtgctttggc gtagttgttt gataatctca aatacccata atttaaaggt
     8401 tttggcttcc ttcttacgac ttgagaagat agtttcataa atgccaaact cattaacgat
     8461 taacatttct tgttgacgcc ctaaactatc tgcgacgtgg ttgtttgaaa caacctcatc
     8521 tcccaaacgt tgtttaataa attttggatt cagatctagt gctttagcaa tatcagctag
     8581 caccgcccac cattcatttt ggtgctctac aaatcggata gtatatccat tccaatgttc
     8641 agttttcatt tgcgtcctcc taatcttcgt gtgtgagttc aattaagtca gttaattgac
     8701 acttaaaaaa aaatacataa actgattaaa gtagatagtt taatatctgt tttgccatta
     8761 ataaatttag aaatggtaga tttggatata cctgtttttt cagcaacttc actagcttta
     8821 gtattattta aagccattac tatatgcaaa ttatttttta ccatgcaaca taattccttt
     8881 cttatatggt taaattatag cgtaatcgat ataacttatg tttatgttaa cagaaatagt
     8941 ttatggtgtc acacgtaaaa aacttatttc atagtttatt ttttttagtt attacactat
     9001 aataaagaaa aatattaagg agtaaataaa tgttaataaa cagattggct atactattgg
     9061 cagaaaggtc gttaagtggt tctcgcttgg cagttgatac tggaattgcg caatcgacta
     9121 tttctaaaat tacttctaac aagtcaaagc aagttgatta tgcgacggta aatactatat
     9181 gtaataactt aaaagttact ccaagtgatt ttttcgaata ttctccaata gattatgata
     9241 tatcgtgtgt gaaggacgaa gatgattctt caatattaat ttttattaaa gtctcaaaat
     9301 ttgacactaa aatttgtacc ttagagttta aagctgttcc gaaatattat gttaaccaag
     9361 atcaacctga tttttttgaa gaatcaagtt tatcaataga taaaataaaa aaagaataca
     9421 caaaagtaga caaattgata attagtcttg aagctaagaa tattaataaa gaaaaatgtg
     9481 acgatttcta tgatttgcct atagtttttc aaaatatttt aaaaaatgat ttaatggaaa
     9541 aagttggtta ttttttaaaa aatgagatta ttaatgtatt ttctgacgaa ttcaccttca
     9601 aaaaaaattc attcggtcgt gttgatttgc tttattttaa tagtaaagc
//
LOCUS       DATRSD010000073         9387 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRSD010000073 DATRSD010000000
VERSION     DATRSD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.074"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            6..1634
                     /locus_tag="VBD85_001596"
     CDS             6..1634
                     /locus_tag="VBD85_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7134139.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            1747..2724
                     /locus_tag="VBD85_001597"
     CDS             1747..2724
                     /locus_tag="VBD85_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134140.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            2721..3542
                     /locus_tag="VBD85_001598"
     CDS             2721..3542
                     /locus_tag="VBD85_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134141.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            3554..4357
                     /locus_tag="VBD85_001599"
     CDS             3554..4357
                     /locus_tag="VBD85_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134142.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSTKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            4341..4967
                     /locus_tag="VBD85_001600"
     CDS             4341..4967
                     /locus_tag="VBD85_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134143.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSRVLAGFLHPKSGEVLVDGSNLPSKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            5250..7280
                     /gene="treP"
                     /locus_tag="VBD85_001601"
     CDS             5250..7280
                     /gene="treP"
                     /locus_tag="VBD85_001601"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO7134144.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPAIFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVPDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDTVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQQEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            7502..9127
                     /gene="treC"
                     /locus_tag="VBD85_001602"
     CDS             7502..9127
                     /gene="treC"
                     /locus_tag="VBD85_001602"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO7134145.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWAPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQDDSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
BASE COUNT         2872 a         1550 c         1897 g         3068 t
ORIGIN      
        1 taatagtgac taaatatttg aagtacatct cttttgttgc tttgtttctg gcgagtatct
       61 ttttagtagc ttgtcaaaat caaaattcac aaaccaagga gcgaacgcga aaacaacgac
      121 ccaaagatga attggttgtt tctatggggg caaagcttcc tcatgaattc gatccaaagg
      181 accgttatgg aatccataat gaaggtaata ttactcatag caccctattg aaacgttctc
      241 ctgaactaga tataaaagga gagcttgcta aaaaatataa aatctctaag gatggcttaa
      301 cgtggtcgtt cgacttaaat gatgatttta aattctccaa tggtgagcct gttactgctg
      361 acgatgttaa gtttacttat gatatgttga aagcagatgg aaaagcttgg gatttgacct
      421 ttattaagaa tgttgaagta gttgggaaaa accaggtaaa tattcatttg actgaggcgc
      481 attcgacatt tacagcacag ttgacagaaa tcccaatcgt ccctaaaaaa cattacaatg
      541 ataagtataa gagcaatcct atcggttcag gaccttacat ggtgaaagaa tataaggctg
      601 gagaacaagc aatcttcgtt cgaaatccct attggcacgg taaaaagcct tattttaaaa
      661 agtggacttg ggtattactt gatgaaaata cagcactagc tgctttagaa tctggtgatg
      721 ttgacatgat ttacgcaacg ccagagcttg ctagcaagaa agtcaaagga acacgtcttc
      781 tagatattgc ttcaaacgac gttcgcggac tgtcattgcc ttatgtgaag aaaggtgttg
      841 tgaaaaattc accagacggt tatccagtag gaaatgatgt cactagtgat ccagcaatca
      901 gaaaagcctt gactattggt ttaaatagac aaaaagttct ggatactgtt ttaaatggtt
      961 atggtaaacc agcttattca attattgata gaacaccatt ctggaatcca aaaacagcaa
     1021 ttaaagataa taaagtagct aaagctaagc aacttttgac aaaagctgga tggaaagaac
     1081 aagcagacgg tagccgtaaa aaaggaaatc ttaaagcgga atttgacctt tactacccta
     1141 ctaatgatca attacgagca aacttagccg ttgaagtagc ggagcaagct aaagccttag
     1201 gtattactat taaactcaag gctagtaact gggatgaaat ggcaactaag tcacatgatt
     1261 cagccttact ttatgcggga ggacgtcatc atgcgcagca attttatgaa tcacattacc
     1321 caagtttagc tggtaaaggt tggaccaata ttacttttta taacaatcct actgtgacta
     1381 agtaccttga caaagcaatg acatctcctg accttgataa agctaacaaa tattggaagt
     1441 tagctcagtg ggatggcaaa acaggtgctt ccactcttgg agatttacca aatgtatggt
     1501 tggtgagtct taaccatact tatattggtg ataaacgtat caatgtaggt aaacaaggtg
     1561 tccatagtca tggtcatgat tggtcattat tgactaacat tgctgaatgg acttgggatg
     1621 aatctgctaa gtaactaatt tagttaatgt aggctacttt aagtttggag tcagagaaaa
     1681 caatgtacac cttatggtgt gaatggtttc ctcgactcct tttttaacaa ttaaggagat
     1741 ttatctgtga aacatattac cactgtagtt atctggaaga tacttaggtg tttgtcactc
     1801 gtttttggag tatcaatttt gacctttatt ttactaaaac aatcaccggt tgatccagtc
     1861 atggcaagtg tcaattatga cacgtcacta acccctgttc agtacaaagc gattgctcac
     1921 cactatggtt tggataagcc agctccggtc cagtatttta tttggttaaa aaattttata
     1981 cagggacatt tagggacttc actagtttat cggcaacctg ttattgatat tattagatca
     2041 cgggcaggtg cctctttcat acttatggga ctctcctggg tcttatcggg tcttattgga
     2101 tttatcttag gaactttatc agctttccat caagggaaat tgcttgatcg aattgtaagg
     2161 tggttttctt accttcagat atcagtacca actttttgga ttgggctcat ttttttatta
     2221 atcttttctg tccagctggg gtggttcccg attggtattt cttccccgat aggtactttg
     2281 agtcaagata ttacgttagc tgatcgtatt aagcacctta ttttacctgt tttcacgcta
     2341 agtattctag gcattgccaa tgtaactctt catactagaa ctaaaatgat gtcggtactt
     2401 tctagtgaat atgtcttatt tgccagagcg cgtggggaaa cggaatggca aatttttaaa
     2461 aatcattgtc ttagaaatgc tatcgtacca gctattacac tgcatttttc ctattttgga
     2521 gaattgtttg gaggatccgt tcttgctgag caagttttct catatccagg actagggtct
     2581 accctaactg aagcaggact taaaagtgat acaccgctac ttctagctat tgtgatgata
     2641 gggacattat ttgtttttgc gggcaatctt attgcggata ttttaaacag cataatcaat
     2701 ccacagttaa ggagaaaagt atgatattga gacgtcgaac tattgtttta tggcaactgg
     2761 gtatcgccat ttctctcatt cttagtattc tagccttaaa tctttatttc catagtactc
     2821 ccttgcaaac caatgcagct ttacggaacc ttgctccttc attaaaccat ctttttggga
     2881 cagatggttt aggtagggat atgtttgtca gaacgattaa aggactttat ttctctctac
     2941 aagtcggctt attaggtgcc cttatggggg tcattctggc gacagttttt ggagtgcttg
     3001 caggtttagg aaatagcatt attgataaaa taatagcatg gttagttgat ttgtttattg
     3061 gtatgcctca tttgattttt atgattctca tttcttttgt tgttgggaaa ggtgctcaag
     3121 gggtcatcat tgcaacggct gttacacatt ggccttcttt agcaaggctt atccgcaatg
     3181 aagtctatca tctaaagaat aaagaatttg tccaactttc taaaagtatg ggaaaaacgc
     3241 cttattatat tgtgaggcat catatcctgc ctttgattgc ttctcaaatt ttcattggtt
     3301 ttatcctctt atttccacat gtcatcctac atgaagcatc aatgactttc ttaggatttg
     3361 ggctctctgc cgaacaacct tcggttggta tcattctgtc agaggcagct aagcatatct
     3421 ctcttggaaa ttggtggttg gttatctttc caggacttta tcttattttg gttgtcaatg
     3481 catttgatac tatcggagaa tctttaaaga aactctttta ccctcaaact gatcattttt
     3541 aggaggtaga gatatgacag aaacattatt aagcattaaa gacctctcca tcaccttcac
     3601 tcaatacgga agatttttaa aaccatttca atcaacaccg atacaagcgc tgaatttaga
     3661 aattaaaaaa ggtgagttat tagctattat aggtgctagt ggttcaggga agagtttatt
     3721 agcacatgct attatggata ttcttcctaa aaatgcatct gtaacaggag atatgattta
     3781 tcgtggtcaa tcactaaatt ctaaacgcat taaacagttg cgaggaaaag atattacgtt
     3841 gattccacaa tcagttaatt atttagatcc atctacgaaa gtcaaacatc aggtgcgctt
     3901 aggtatctca gaaaattcaa aggctactca agaaggattg tttcaacagt ttggtttaaa
     3961 agaaagtgat ggtgacttgt atcctttcca actttctggc ggaatgctcc gacgtgtttt
     4021 gtttacaacg tgtattagtg ataaggtttc tttgattatt gcggatgagc ccacccctgg
     4081 attacatcca gatgctctgc aaatggtttt agaccaacta cgctcctttg cagataaagg
     4141 aataagcgtt atatttatca ctcatgatat tgtagcagct agtcaaattg ctgatcgtat
     4201 tactattttt aaagagggaa aagctattga aacagctcca gctagtttct ttagcggaaa
     4261 tggagagcag ttacaaacag aatttgctag aagtttatgg cgctctctcc cacagcaaga
     4321 atttttgaaa ggagttactc atgaccttag aggctaaaaa gcttggcttt tatcataaaa
     4381 aagatcaatg gctttttaag gagattaatt tagaggtagc acctggtcaa gttttaggta
     4441 tatttggaca aagtggttgt ggaaaaacta gtttatccag ggtgcttgca ggttttttac
     4501 atcctaaatc tggtgaagta ttagttgatg gcagtaattt gcctagcaaa gcatttagac
     4561 ctgtacaact tattcaacaa catcctgaaa aaacgatgaa tcctttatgg cctatgaaaa
     4621 aaagtttgga agaagcctat tacccaagtc gagatttact agatgctttt ggaattcaag
     4681 aaaaatggct aaatcgtcga cctagtgaac tctcgggagg agaattacaa cgcttttcga
     4741 ttgtgcgttc attacatcca gagactaaat accttattgc agatgaaatg actactatgt
     4801 tggatagcat tacacaagct agtgtatgga aaagcctgtt ggagattgta aaggatagaa
     4861 atttaggtct catcgttatt agtcatgatt tcgctatgtt agagaaactt tgtaaccaat
     4921 gctatatgat tgaagagaat cgtatcgtat ctttcaatgg agattaaaaa actcacctac
     4981 tatatcaatt agtaggtgtt ttttattaag agagtattga gggtgactga tctactcatg
     5041 actctaacat tttggtaacg ttggtgagaa attctaatgt tttgggtatt attgactaat
     5101 ttattttata atttatagat tttttaaaaa gataagctaa ggatttgcat agggatatgt
     5161 aatcgtttac aattatgttg taagctagtc ttatttaagt ttctgccttg gagtcacaga
     5221 ctctccgtta aatagataag gataaacaga tggaacaatt taaacatgat gccaaagcct
     5281 tgttagaggc aattgggggt aaagaaaata ttagtgcagt gacacattgt gctacacgca
     5341 tgcgatttgt tttgaatgac agtagtaaag ctaaggtaaa ggtaattgag gagttaccat
     5401 cagtcaaggg tacctttacc aatgctgggc aatttcaagt cattattggt aatgatgtcc
     5461 ctatttttta taatgctttt gtagccgttt ctggtattga gggtgtgtct aaagaggcgg
     5521 caaaatcggc tgcccaaaaa aatcaaaacc ctttacagcg tgtgttgaca atgctggctg
     5581 aaatatttac accgattatt ccagccatta ttgttggtgg tctgatcctt ggttttcgta
     5641 atattttaga tgccgttcca tttgaatttt taggtcaaaa agttgttgat ggtgtcaggc
     5701 aagtagactc ttctgggcat ccaatttgga atactctagt agatgtttca acgttttgga
     5761 gcggagtaga ctctttttta tggttaccag gtgaagctat atttcatttc ttaccagttg
     5821 gtattgtatg gtctgtgact cgtaagatgg ggactactca aatcctcggt attgttctag
     5881 gtatctgtct agtgtcgcca caattactta atgcttattc tgtagctagt acttcagcag
     5941 cagacattgc taaaaattgg tcttggaatt ttggttactt tacagtacaa aaaattggtt
     6001 atcaagctca agttattcca gctctgttag ctggtttgag cctgtcttac ttagaaatct
     6061 tctggagaaa acatattcct gaagttgttt caatgatttt tgtgccattt ctatccttag
     6121 ttccagctat tatcttggct catactgtcc ttggacctat tggttggaca ctaggtaaat
     6181 ggatttctgc tatcgttctt attggattga ctggtcctgt aaaatggctc tttggtgcta
     6241 tttttggtgc cctgtatgcc ccatttgtta ttactggttt gcaccacatg acaaatgcta
     6301 tcgatactca actcatagca gatacaaaaa cacatacaac tggcctttgg ccgatgattg
     6361 ctctttcaaa tattgctcag ggttcagctg tactagctta ttattttatg catcgtcatg
     6421 atgaaaaaga ggctcaaata tcacttccag ctgctatctc agcctacctt ggtgttacag
     6481 agccggcgat ttttggtgta aacgttaaat acatttatcc atttgttgct ggtatgattg
     6541 gttcaagtgt ggcaggtctt ttggcgacaa cttttaatgt ccaagctaac tctattggtg
     6601 taggcggtct tccaggcttc ctgtcaatta acgttaaata tatgggatat ttctttatct
     6661 gtatggcagt ggctatcttt attcctttat ttttgacact attctttaaa aaatcaggta
     6721 ttttgacaaa aactgaagag gaaaaattag tacctgatgc tgtcattgct tcaacaacag
     6781 aaacaaaatc agcaaaagaa aaagctgtcg tatcaggtac aaagcttagt gtagtaagcc
     6841 ctttatcagg tttagctaaa ccactggatc aagctagtga tcctgtcttt tcacaaggaa
     6901 ttatggggaa gggagtggtt atcgatccaa gtgatggaga actagtttca ccggtcgatg
     6961 cgacagtttc agttcttttc cctacgaaac atgctattgg attgttaaca tcagaggggg
     7021 ttgagtttct tattcatatt ggtatggata ctgttaattt agaaggaaaa gggtttacct
     7081 ctcatgttgc tcaaggtgac actgtaaaag taggagataa actcattact ttcgatattc
     7141 caatgataaa agaagaagga tacattgtgg aaactcctat tctgattact aatcaacaag
     7201 aattccgacc agaagaattg atagatttac ccaaacagat aaaacgtgga caagcattaa
     7261 tggttgccaa gaaaatttaa aagtaattag gtattctttt taaaaaagag cactcttata
     7321 aaatgagagg taatttgagg aattgtttga ggggcttaat acattagctc cttgaattat
     7381 tttgaacata agaaaaaact cataggaaca aatatcactg taaaaatgtt atatttttga
     7441 tgatagttga tgttatagtt agactgctaa atacagcagc attttgggaa aggagtagaa
     7501 tatgacgatt gataaacgta aagttgtcta tcaaatttat ccaaaatcat acaaagacac
     7561 aactggtaat ggggttggtg atcttcgtgg tattatcgaa aaattaccct atttagcaga
     7621 acttggtatt gatatggttt ggctcaaccc attttatcca agtccacaac gagataatgg
     7681 atatgatatc tctgattata cagctattaa ccctgatttt ggaacgatgg acgattttga
     7741 agaaatgatt gaggtgggac gtcaataccg tatagatttt atgcttgata tggtgcttaa
     7801 tcattgttca atagaacatg agtggtttaa aaaagcactt gcaggagatc gctactatca
     7861 agatttcttt atcttacgtg ataatccaac agattgggta tctaaatttg gtggaaatgc
     7921 ttgggcacca tttggtgata cagggaagta ttatcttcat ctttttgata tcactcaagc
     7981 tgaccttaat tggcgcaatg ccgatgttcg caaggaactt tttaaagttg tcaatttttg
     8041 gcgtgataaa ggggttaaag gttttcgatt tgatgtgatt aatttaattg ggaaagatga
     8101 gatacttgag aactgcccaa ttaatgatgg aaaaccagct tatactgatc gtccaataac
     8161 acatgattat ctcaagatgt tgaataatgc tagttttgga caagatgatt ccttcatgac
     8221 agttggtgaa atgtcttcaa cgacaattgc aaattgcatt ctttacactg caccagaacg
     8281 tgaagagtta tccatggctt ttaattttca ccacctcaaa gtagattata aagatgggca
     8341 aaagtggacc attatggctt ttgattttcc tgcattacgt gaccttttcc atagttgggg
     8401 cgaagggatg agcgaaggaa atggttggaa tgctttattc tataataatc atgaccaacc
     8461 gcgagcatta aatcgttttg tagacgtgaa acgttttcga aatgaaggag caaccatgtt
     8521 agcagcatct atccacttat cgcgtggtac tccttatata tatatgggtg aagaaatcgg
     8581 aatgcttgat cccgattaca gttctatgga tgattatgtg gatattgaga gtttgaatgc
     8641 ctaccaaata atgcttgatg aaggcaagtc acaagaagag gcatttagca ttattcgagc
     8701 gaaatctcga gacaattctc gagtaccaat gcaatgggat gatagtacga atgcaggttt
     8761 ttctgaaggc gctccatggt tgaaagtagg aaaatcatat aaagaaatca atgtcgcaaa
     8821 ggagaaaaca gggctgatat ttactttcta tcaagagttg ataaggctac gtaaacaact
     8881 gccaataata gctgacggga attataaagc agctttcaaa gataatgaaa aagtatatgc
     8941 ttttgaacgc catcttgata aggaaaaact tcttgtttta aataatttct ttgctgagaa
     9001 agttaagata aaattaccgg agaattatct ccaaggacaa gttcttctat caaattataa
     9061 ggatgttact cttgatgaaa cggttacttt gcaaccttat caaactttag ctatcttagt
     9121 gtcataacat tttaaaaacc tttacttatt caattaagta aaggttttta aagtgctgct
     9181 tgtggaaagg aatcttgctc tagattttgc aatttctttc ttttttcttt actctataac
     9241 aatagcccaa attcattcaa ttgatagtag tgccagattg ggctcaaatg acctagaaaa
     9301 atgtaaacgg ttactataaa ataaaaatag aaaggaaaac aaatggttga taataagact
     9361 gttgtgataa tgttggtatt tttagct
//
LOCUS       DATRSD010000074         9347 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRSD010000074 DATRSD010000000
VERSION     DATRSD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.075"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..576)
                     /locus_tag="VBD85_001603"
     CDS             complement(<1..576)
                     /locus_tag="VBD85_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO7134146.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIF"
     gene            complement(666..1250)
                     /locus_tag="VBD85_001604"
     CDS             complement(666..1250)
                     /locus_tag="VBD85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134147.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            1339..2151
                     /locus_tag="VBD85_001605"
     CDS             1339..2151
                     /locus_tag="VBD85_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7134148.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFERSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNFSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNANPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            2453..3463
                     /gene="manX"
                     /locus_tag="VBD85_001606"
     CDS             2453..3463
                     /gene="manX"
                     /locus_tag="VBD85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO7134149.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAIEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            3496..4308
                     /locus_tag="VBD85_001607"
     CDS             3496..4308
                     /locus_tag="VBD85_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO7134150.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            4323..5234
                     /locus_tag="VBD85_001608"
     CDS             4323..5234
                     /locus_tag="VBD85_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7134151.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            5353..5715
                     /locus_tag="VBD85_001609"
     CDS             5353..5715
                     /locus_tag="VBD85_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO7134152.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(5753..6814)
                     /locus_tag="VBD85_001610"
     CDS             complement(5753..6814)
                     /locus_tag="VBD85_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7134153.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWAQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIIY
                     LLSLVATYAMKFSVGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            7111..8388
                     /gene="serS"
                     /locus_tag="VBD85_001611"
     CDS             7111..8388
                     /gene="serS"
                     /locus_tag="VBD85_001611"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO7134154.1"
                     /translation="MLDLKRIRTDFDVVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     AEEAKAQRNVASAAIAQAKRNKENADEQIAAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            complement(8434..9066)
                     /locus_tag="VBD85_001612"
     CDS             complement(8434..9066)
                     /locus_tag="VBD85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO7134155.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
BASE COUNT         3139 a         1758 c         1594 g         2856 t
ORIGIN      
        1 aaaaataaga cacaatacct gtaaaaatgt aaatcctaaa ataaagtgga atgaatccct
       61 actcggaacc gctaaaagtt cgttaattac cacttgtggc ttcgttaaga tatcccatga
      121 atttagacgg gcataacgac caatatgaat accaaaactt gaaacaaatg atacaattcc
      181 aataattccc catcttaaaa atatattgct aattctaaat ttacgcataa tcacagaaaa
      241 actttcgata cctgataaaa aaccaaacaa gatactagat acatacaaga tataaagtat
      301 taaatttgtc ttattataaa gaacgtctcc gacaaaatgc atatgaatga tatccgtcaa
      361 catataaaag gtattaggat agaaaaaaaa ccaagcaagt gcaaaaagac cgataagaat
      421 ttttttctta aataaactag taaggtatgc aaaatctaac gctattaggg ctagagtcat
      481 attccaaatc aaatttggtc cctgcaaatg ataatattta atcccaaaag aaataacaat
      541 aaaaaataga tgtatcgcaa atagttttac tttcattcta acctccaatt aatatctcat
      601 tatacaacat attattaaat taaggttaaa taaacttcaa acctaaaaga ctaaccctgt
      661 tgtggttagt cttccattaa tattccccaa agtaataatt gtactaaaaa aaggcaagac
      721 aaggctttaa aatcaaggtt agccatatta gtctgtattc cattaaaaaa tgctgacgga
      781 tttaaaatga ttccttctaa acctaatgat cctgattttt ctaagcaaaa ggcaaaacta
      841 ctaaaaagca ttacaggtat aacagttgct aattgatacc ttttactaat atcatatgca
      901 tttaccaata gcttcaagct tagtaaagct gaagagataa taaaaagaat tgcaagaagg
      961 tatgccatat attgcataaa gtgattagac tgccaaatgt cttttgttga ccactctaag
     1021 ctcaccaaac taagaaaaat ccctaaactc tgtgggtaaa aaaataaaag acctaaactt
     1081 gctaaaagac gaataaaaat attacgacta taataagcaa tgaatcccat ctctaacgac
     1141 aagactgatg ccaatagaat taccatcaaa tgatcaattt ttacatgata ataccatatc
     1201 cctaaaatac tgactatggc aaaaagatga ctccaaatta actttttcat aatccaccaa
     1261 cttccatatt tccacagtta ttatatcata tttctactgt ctctttttat tgaaatctat
     1321 ttttgatata atgtcagcat gacaaagaaa attatcgccg tagatttaga cggtactcta
     1381 ttacataata ataacactat ttctgactac accgctgata cacttcgaaa agttcaagct
     1441 caaggacaca aagtaattat tacaaccgga cgcccatacc gtatggcact cgctcattat
     1501 ttacgcctag atttaaaaac accaatgata aattttaatg gtgctttaac tcatatcccc
     1561 gaaaaaaagt gggcgtttga gcgtagtgca acgattgata aaaaattact ccttgaaacc
     1621 ttaaatttat cagatgctat ccaagccgat tttattgcta gcgaataccg taaaaatttt
     1681 tacattacta tggataatcg tgataaaatt aacccacaat tattcggtgt caatgaaatt
     1741 actgataaaa tggcattaga tgttactaaa atcacacgca atccaaatgc tctattgatg
     1801 caaacacgtc ataaagataa gtatgagtta gctaaagagt tacgacaaca ttttaatcac
     1861 gaactagaag tagattcttg gggtggtcca ttgaatatac tcgaattttc acctaaaggt
     1921 gttaacaaag cttatgctct caaacattta ttaaaatcac tcaatttttc acaagaaaat
     1981 ttgattgcct ttggagatga acacaatgac accgagatgc tggcctttgc acatacagga
     2041 tatgctatga aaaatgctaa tccaactcta cttccatatg ccgaccaaca aatccaatgg
     2101 acaaacgaag aagatggtgt tgctaaaact ctagaaaaat tattattgta gtattttcaa
     2161 aaatgatctc tattgaaaca gaattctaat agaaatcgtt ttttaggttt tctactatat
     2221 tattaacaaa aaaagttacc actctcttcc cctattataa aaggtatttg tgaaaaaaat
     2281 atcttttttt taatttttat tagaaatagt attgattttt ttctgaaaac gttttaaaat
     2341 atatctgttc tataatttcg ttgaaatgaa aaagaatggc caattttacg attggaagga
     2401 gattattctt ctttatgtca acgaaaaaat tttttaagga ggaagaacaa gaatgggtat
     2461 cggtattatt attgccagcc atggtaaatt tgctgaaggt attcatcaat caggttcaat
     2521 gatctttggt gaacaagaaa aagttcaagt tgtaactttc atgccaaatg aaggaccaga
     2581 tgatttgtat ggacacttca acaatgctat cgcacaattt gatgctgatg atgaggtgct
     2641 tgtgttagct gacctttgga gtgggtcacc atttaaccaa gcaagccgtg ttatgggaga
     2701 aaatcctgaa cgtaaaatgg ctatcatcac tggtctcaat ttgccaatgc ttatccaagc
     2761 ctatactgag cgtatgatgg acgctaatgc aggtgttgaa caagttgccg caaatattat
     2821 taaagagtct aaggaaggta tcaaagccct tccagaagaa cttaatcctg ttgtagaagc
     2881 tactcctgta gcaggtgttc ctgcagatgt tcctgcggaa gttaaacaaa gtggatctat
     2941 ccctgaagga actgttattg gagatggcaa gcttaaaatt aatcttgctc gtattgacac
     3001 acgtctttta catggtcaag ttgcaactgc atggactcca gcatcaaaag caaaccgtat
     3061 tatcgttgct tctgatgaag tttctaaaga tgaacttcgt aaacaattga ttaagcaagc
     3121 tgctcctgga ggagtcaaag caaacgttgt cccaattagt aaattaattg aagtagctaa
     3181 agacccacgt ttcggaaaca cacgtgcctt gatcttattt gaaactgtac aagatgcact
     3241 ccgtgcaatt gaaggtggtg tggaaatccc tgaattgaac gttggttcaa tggctcactc
     3301 aactggtaaa acaatggtaa acaacgttct ttcaatggat aaagacgatg ttgctgcctt
     3361 tgaaaaatta cgtgacttag gtgtatcatt tgatgtacgt aaagtaccaa atgacgctaa
     3421 aaagaatttg tttgatttaa tcaacaaagc aaacgtaaaa taacttatta tctctgaaaa
     3481 cgaaaggata tcaaaatggc ggatatttca attatctcaa tgattttggt cgttatcgtt
     3541 gccttctttg ctggtcttga aggtatcctc gaccaattcc aaatccatca acctcttgtt
     3601 gcttgtactt taatcggtct tgtaactggt cacctcgaag cgggtattat tcttggtggt
     3661 agtctccaaa tgattgctct tgcttgggca aatatcggag ctgccgtagc accagacgct
     3721 gcacttgcct ctgttgctgc tgctattatt ttggttaaaa gtggtgactt cactcaaaaa
     3781 ggtatcaact ttgccttctc tactgctgta cctctagcta ttgctggact tttccttaca
     3841 atgattgttc gtacaatctc aactgcactt gttcacgctg gtgacaaagc tgcttctgaa
     3901 ggaaacttcg cagcaattga acgttttcac tttatcgccc tccttctcca aggattacgt
     3961 attgcattcc cagctgcgct tcttttagct attccatctt cttcagtaca aagtattctt
     4021 gaagcaatgc ctgattggtt aaatggtggt atgcaagttg gtggtgccat ggttgttgcc
     4081 gttggttacg ctatggttat caatatgatg gctactcgcg aagtttggcc tttctttgct
     4141 cttggatttg ctctagcagc tttgaaccag ttaactctta ttgcaatggg tactattggt
     4201 gttgctatcg ccctcatcta catcagttta tcaaaaatgg gtggaagcaa aggtacttct
     4261 aacgcaggtt ctaacgatcc tatcggcgat atcttagagg actactagaa aggagctaaa
     4321 caatgactga acaaattaaa ttatcaaaat cagatcgtca aaaagtttgg tggcgttcac
     4381 aattccttca aggttcttgg aactatgagc gtatgcaaaa tatggggtgg gcctatgctc
     4441 ttatcccagc attgaaaaaa ctttacacta ctaaagaaga tcgtgctgct gcgcttgagc
     4501 gccatatgga attctttaac acacatccat acgttgctgc tccaattatc ggtgtaaccc
     4561 ttgctcttga ggaagaaaaa gcaagcggta cacctgtcga agacaaagca attcaagggg
     4621 ttaaaatcgg tatgatggga cctcttgctg gtattggtga tccagtattc tggtttactg
     4681 tacgtcctat cttaggtgct cttggtgctt cacttgcctc tgctggtaat atcctcggac
     4741 caatcatctt ctttgttggt tggaacctca ttcgtatgtc attcttatgg tacacacaag
     4801 agttaggtta caaatcaggt aaagaaatta ctaaagatat gtctggtggt atcctccaag
     4861 atattactaa aggtgcttca atccttggta tgttcatcct tgctgtcctt gttaagcgtt
     4921 gggtagcaat taactttact gtagaccttc ctaaaaagac attgtcagaa ggggcataca
     4981 tcaatttccc taaagaccat gttagtggac aacaattgca tgatatccta ggacaagttc
     5041 agagtggctt aagccttgat aaaatgcaac cacaaacact tcaaggacaa cttgactcac
     5101 ttattcctgg tttagctgga ttacttctta cattcttctg tatgtggtta cttaagaaga
     5161 aagtatcacc aattacaatt atcatcggtt tgtttatcgt tggtattcta gctcgccttg
     5221 caggtgtgat gtaataaaac taagagatgg ttacaatagt attccatctc ttatatttta
     5281 ttacataaaa tccacttaga gtcccactat tatgatataa tcaaactact ataaattaaa
     5341 aagggattag tcatggcaca atcattaaac aaaactgttg aatttcaaac aacaggagtt
     5401 tcgtatttag gtatggggaa taaagttgga aaatttttag ttggtgacca ggcattagaa
     5461 ttctataacg ataaaaatgt caacgactat attcaaattc catggacatc aatcaatcaa
     5521 attggagcta atgttagtcg aaaaaaaatt agtcgtcact ttgaggtctt tacagaccaa
     5581 ggaaaatttt tattcgcctc taaagactct ggaacgatat taaagcatgc gagacgacat
     5641 attggagacg ataaagttgt taagcttcct accctcatcc agaccatttt aaaaatattc
     5701 aagtttaaaa aataagatag actatagtct atcttatttt ttaactctaa tattaatatg
     5761 ggaacttgtc atcaaaccat ctcaaaaagt tagaaaaatg gattgcttta aatattcgta
     5821 atctaatacg ttctacaata aaagccaaca aataaattaa aatccctgct cctaaaatta
     5881 aaaatgggta aagataaatg gactcataaa caaacgtctc tgcaaaatct cttacaaaat
     5941 attttactat tagtgggtgg agatgacaga gataaactcc caaggttgaa ggggctaata
     6001 caacaattat tttctttaag aatcccgagg gcttaatgct tgctctagca aataatataa
     6061 acaaacttac cgcccctaaa gttaaagtag gcgaaacata ccaataccaa atatcaccaa
     6121 cgctaaactt catggcataa gtagcaacca atgataatag ataaattata agtaaatatg
     6181 acgttttaaa tatatttaaa tcaatacgtt tcaaataggc accaataata tataaaatca
     6241 gtagccaagt catttcaaaa cccttgctca atgaaaattc tggaacacga ttatttaaaa
     6301 cagctggtaa aattgaaaaa ataataaaca ttaacaaaac caattgtttt aattgtttat
     6361 cagtcaaggc atttaatcca ttattaataa caggcataaa aaccaataga ccaaagtaag
     6421 cagtaatgta ccaatactgc ccactgacaa tagggaaaaa agcatctctc caattaagca
     6481 aggtcacttc atgacctgtg attgcaaaaa gtgctgttat tgtaaaggtg tagaaaaaaa
     6541 cctgtgccca gatagacagt aattttgagt aacgataacg cgaattaatt cccacataac
     6601 cacttattag tgcgtaacag ttaacggccc cataaactaa gacttggata atccacgtta
     6661 ctataaaata ggagtctgcg tgtccctcaa ccgaagatct cagaccacct tttccaagaa
     6721 catgcgtaat aacaatcatg aacatggaaa taatccttaa aaggtctaag ccgtaatgac
     6781 gacctgattg tttaatactt gtatactttt ccataatcat attttagcat agctatttca
     6841 ctaaaacaaa gagcaggctt ataaagttaa ggtttacatt ttacttattt ttggatataa
     6901 taaccttaac ggataagtag tataatagtt cttttcagag agtctgcggt cgttgcgagc
     6961 agataagatt gttttatgaa attctaccgt gttccattta tgggaaataa tttaatcaat
     7021 caatcaagtg cacttctgtg aagctggatg gaaccgcgcc tttgcgctcc agcatgacag
     7081 ggtgtttttt agtataaagg agattcctat atgttagact taaaacgtat tcgtacagat
     7141 tttgatgttg tcgctaaaaa attggccacg cgtggtgttg accaagaaac attaacaacc
     7201 cttaaagaac ttgatataaa acgccgtgaa ctacttatca aagctgaaga ggcgaaagcg
     7261 caacgtaatg ttgcttctgc tgccattgct caagcaaaac gaaataaaga gaatgcagac
     7321 gagcaaatcg cagctatgca aacactttcc gctgatatca aagcaattga tgcggaattg
     7381 gcagatgtgg atgctaattt acaatcaatg gtgaccgtac ttcctaatac accagctgat
     7441 gatgtgccgc ttggagcaga cgaggatgaa aatgttgaag ttcgtcgctg gggaactcct
     7501 cgtgaatttg attttgagac caaagctcac tgggatttag gtgaaagcct tggaattctc
     7561 gattgggaac gtggtgcaaa agttactggc tctcgcttcc tcttctacaa aggtctaggt
     7621 gctcgtttag agcgtgctat ctacagcttc atgctggatg aacacgctaa ggaggggtac
     7681 actgaagtga tccctcctta tatggttaac cacgattcaa tgttcggtac gggacaatat
     7741 ccaaagttca aggaagatac tttcgaatta gcagatagcc cattcgtact tatcccaaca
     7801 gctgaagttc ctttaacaaa ctattatcgt gatgaaatta tcgatgggaa agaattacct
     7861 atatacttta ctgccatgag tccatcattc cgttcagaag caggctctgc aggacgtgac
     7921 acacgtggat taattcgcct tcatcaattc cataaagtag aaatggtgaa atttgccaag
     7981 cctgaggaat cataccaaga attagaaaaa atgacagcaa atgctgaaaa tattcttcaa
     8041 aaacttaatc tcccataccg tgtcattacc ttgtgtacag gagatatggg attctcagca
     8101 gctaaaacgt atgacttaga ggtatggatt cccgctcaaa atacttatcg agaaatttct
     8161 tcatgttcta atactgaaga tttccaagca cgtcgtgctc aaattcgtta ccgtgacgaa
     8221 gttgatggca aagttaggtt gcttcatact ttaaatggtt caggacttgc agttggacgt
     8281 actgtagcag caatcctcga aaattatcaa aatgaagatg gttctgtgac tatcccagaa
     8341 gttcttcgtc catatatggg taatatcgat attattaaac caaattaggc actctaaaat
     8401 gagttggtat accaactcat tttttctatg aacttagcta atagtttgac aaacttgata
     8461 gtaaggtaac actttctcaa agccttctaa caatcgttgc agaatatttt ctaagctctg
     8521 gttttcagtc aaaagaacgt catattttac caatactttc cttaccttcc ctgacttaat
     8581 ttcttgcatt aaacgttgac gattttcctc attgccttct tctcgatgag tttcaccttg
     8641 cctttggacc ataaaataca atggagaggc aatcggaatc gataacactc tagcctgctt
     8701 ctcgagtgtc ttatcagatt tttgcctctc tacaaagctt acctcaaggc taaccccaaa
     8761 actatctttt acaccataaa cccttagcgc aacagccaca tcatctaatt ggtctgtatc
     8821 ctttcgataa taaacccaaa aatgtggacg ccctacttga gcttgattag cccaattact
     8881 cactctttgc attttaaagc catccatacg ttgttctaag gctcttgata actctgtaaa
     8941 gctattccta gcaagttggc cctttgcctt aaaagtttgc atatcgtcct ttaaagggcc
     9001 tgccttctca ggcataatat acttcattga ttggaattct aaataatcat ttaattcttg
     9061 atacattctt tttctccatg taaacaatag ttaagttttc acgattatat atatctatac
     9121 atactttcaa gttcttttaa agtatttcta gcgaagcctc gttgctagaa acaaggtcta
     9181 atcgcaatta taactaacca agctttactt tcttcatcac ttaatggaac tctttaaaaa
     9241 ccaatctttt catagccttc taaaatctaa taatagctat tttttctaga aaatttctct
     9301 tcaataacag atagagtctc aagttaagga gagcctaaat aatggta
//
LOCUS       DATRSD010000075         9239 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRSD010000075 DATRSD010000000
VERSION     DATRSD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.076"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..1290
                     /locus_tag="VBD85_001613"
     CDS             <1..1290
                     /locus_tag="VBD85_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO7134156.1"
                     /translation="LLILTLFNAFFSASEMALVSLNRSKVEQKAEEGDKRYRRLLDVL
                     ENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATARTAGSIIALIFLTYV
                     SIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLSASTNLLSRITPMTFD
                     DADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMAREVMVPRTDAFMIDINN
                     DAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKTGFDTIDLRKILQEPL
                     FVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLEEIVGEIDDETDTAEQ
                     FVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLTGVGSIPNQEEKVAYE
                     VDSKDKHITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1358..2146
                     /gene="pflA"
                     /locus_tag="VBD85_001614"
     CDS             1358..2146
                     /gene="pflA"
                     /locus_tag="VBD85_001614"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO7134157.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILDKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2263..3198
                     /locus_tag="VBD85_001615"
     CDS             2263..3198
                     /locus_tag="VBD85_001615"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO7134158.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3258..3911
                     /locus_tag="VBD85_001616"
     CDS             3258..3911
                     /locus_tag="VBD85_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO7134159.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDGVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSDSQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNGYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(3940..4941)
                     /locus_tag="VBD85_001617"
     CDS             complement(3940..4941)
                     /locus_tag="VBD85_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7134160.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVINVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIVTGIGIS
                     TMISGIMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLTYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(4938..5963)
                     /locus_tag="VBD85_001618"
     CDS             complement(4938..5963)
                     /locus_tag="VBD85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7134161.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGSGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(5979..6911)
                     /locus_tag="VBD85_001619"
     CDS             complement(5979..6911)
                     /locus_tag="VBD85_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7134162.1"
                     /translation="MKKIGIIVLTLLTFFLVSCGQQTKQESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(6935..7729)
                     /locus_tag="VBD85_001620"
     CDS             complement(6935..7729)
                     /locus_tag="VBD85_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134163.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(7885..>9239)
                     /locus_tag="VBD85_001621"
     CDS             complement(7885..>9239)
                     /locus_tag="VBD85_001621"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO7134164.1"
                     /translation="TFNALSYDSRQISSDTLFFAKGATFKKEYLDSAITAGLSFYVSE
                     IDYGADIPVILVNDIKKAMSLISMSFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKV
                     NHRPAMLSTMNTTLDGKSFFKSHLTTPESLDLFRMMATAVENQMTHLIMEVSSQAYLT
                     KRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIV
                     KEQVEYIPHDFYGDYSENVITESKAFSFHVKGKLENTYDIKLIGKFNQENAIAAGLAC
                     LRLGVSIEDIKNGIAQTTVPGRMEVLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKG
                     DIILVLGAPGNKGQSRRKDFGDVINQHPNLQVILTADDPNFEDPLVISQEIASHINRP
                     VTIIIDREEAIANASTLTNCKLDAIIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLKR
                     "
BASE COUNT         3024 a         1478 c         1722 g         3015 t
ORIGIN      
        1 ttattaatat taaccctatt taacgccttc ttttcagcat ctgaaatggc tttagtatcg
       61 ctaaaccgtt ctaaagtaga acagaaagct gaagaaggag acaaaaggta tcgacgtctt
      121 ttggacgttc ttgaaaatcc caataatttt ttatcaacaa ttcaagttgg tattactttt
      181 attagccttt tgcaaggggc tagtttatct gcctcactag gacatgtgat ttcaggatgg
      241 ctaggaaatt cagcaacagc ccgtacagca gggagtatta ttgcgctaat ctttttaaca
      301 tatgtctcta ttgttttagg agaattatat cctaagcgta ttgctatgaa tttgaaagat
      361 cgtctagcaa ttgtttcagc tcccattatt atctttctag ggaaaatcgt aagccccttt
      421 gtatggttgc tctcagcttc aactaactta ttgagtagaa tcactccgat gacttttgat
      481 gatgctgacg agaagatgac acgtgatgaa attgaatata tgttgacgaa tagtgaagaa
      541 actttggatg ctgaagaaat tgagatgctg caagggattt tctcgctaga tgaaatgatg
      601 gcgcgtgaag ttatggttcc gcgtactgat gctttcatga ttgacatcaa caatgatgca
      661 caatcaaata ttgaaggaat tttatcacaa aacttttctc gtgtccctgt ttatgatgac
      721 gataaagata gagttgtagg tgttttacat accaagcgct tattggaagc gggctttaaa
      781 actggttttg atactatcga cttgcgaaaa atacttcagg aacctctttt tgttccagaa
      841 acgatttttg tagatgacct tttaaaagca ttacgcaaca ctcaaaatca aatggccatt
      901 ttacttgatg aatatggtgg cgttgctggt ttagtaactc tagaggattt attggaagaa
      961 attgtcggcg aaattgatga tgaaacagat acagctgaac aatttgtcag ggaaatcgat
     1021 gagaatatct atattgttct aggaactatg acgcttaatg aatttaatga ttattttgag
     1081 acagagttag aaagtgatga tgttgataca attgcgggtt attatttaac tggtgtgggt
     1141 agtattccaa accaagagga aaaagtagct tacgaagtag acagcaaaga taaacacatc
     1201 actttgatta atgataaagt taaagatggt cgtataacaa aattgaaggt gctattgtct
     1261 gatatagaac agaatattga aaaagactaa gattacttag tctttttttc atgaaaatga
     1321 tataataaag tcataaaaac ggttacaagg tgatgaaatg gcagaaatag actataaaaa
     1381 agtaacagga atgatccatt ctacagaaag tttcggctct gtggatgggc caggtattcg
     1441 ttttatcatt tttatgcaag gatgtaaaat gcgttgtcaa tactgtcata atcctgatac
     1501 ttgggaaatg gaaactaaca attcaaagga acgtaccgtt gaggatgttc taaaagaagc
     1561 tcttcgttat aaacattttt ggggaaaaga tggaggtatc acagtttcag gtggtgaagc
     1621 aatgctgcaa attgacttta ttacagccct ctttattgag gctaagaaac taggtattca
     1681 tacgacgcta gatacttgtg gttttgctta tcgagcaaca ccagaatacc atgctatcct
     1741 tgacaaattg ttagatgtta ctgatttggt actccttgac ttaaaagaaa ttgatccaga
     1801 acaacataaa attgtaactc gtcagtctaa caaaaatatt ttacagtttg ctagatattt
     1861 atctgataga ggtactccgg tatggattcg tcatgtttta gttcccggat tgactgatat
     1921 cgatgaccat ttaaaacgtt tgggtgaatt tgtccaaaca cttgataatg tcgataaatt
     1981 tgaagtattg ccttatcata ctatgggaga gttcaaatgg cgtgaattag gaattccgta
     2041 tccattagct ggtgtgaaac caccaactcc agaacgtgtt aaaaatgcta aagatatcat
     2101 gaaaacagaa tcctatacag aatatctaaa aaggattcaa aattagtttt aagttgaatt
     2161 ttattcaact tttttagttg caaagggctt cctagtaaga ttttttgcta agttattcaa
     2221 tttttgctac aataaggtga atataaaaga agaggtaaaa acatgtctaa aattttagtc
     2281 tttggtcacc aaaatccaga ttcagatgct attggatcat cagttgcttt tgcatatcta
     2341 gctaaagaag cttgggggct tgacacagag gccgttgctt taggaactcc aaatgaagaa
     2401 acagcatatg ttttagatta ttttggtgtt caagcacctc gagtagtaga atctgcaaaa
     2461 gcagagggtg tagagacggt cattttaact gaccataacg aattccaaca atctatttct
     2521 gatattaaag atgtgacagt ttatggtgtt gttgatcatc atcgtgttgc taactttgag
     2581 actgctaatc cgttatatat gcgtttggaa ccagttggct cagcatcatc gattgtttat
     2641 cgtatgttta aggaaaatgg agtatcagta cccaaagagt tggctggttt attattatca
     2701 ggtttgattt cagatactct tcttttgaaa tcaccaacaa ctcatgcttc agatattcca
     2761 gttgcaaaag aactagcaga gcttgcaggt gtaaacttag aagaatatgg tttagaaatg
     2821 cttaaagcag gtactaacct ctcaagtaag actgcagcgg agttgattga tattgatgct
     2881 aaaacatttg aattaaatgg agaagctgtt cgtgttgcac aagttaatac tgtggacatt
     2941 aacgatattt tagcacgtca agaagagatt gaagttgcta ttcaagaagc aattgtcaca
     3001 gaaggttatt ctgattttgt attgatgatt actgacattg ttaattcaaa ttcagagatt
     3061 ttagctctcg gttcaaatat ggctaaagtt gaagcagctt ttgaatttac acttgaaaat
     3121 aaccacgctt ttcttgctgg ggcagtatca cgcaaaaaac aagttgttcc tcaattgaca
     3181 gaaagctaca atgcttaatt aatcatgtaa aacgggctac gtcccgtttt tttagtcata
     3241 catttaggag cgcttttatg atattaacgt ttaatccagg aaagttagaa agacaagagt
     3301 tttttaagga gcttattaac tatctttgga tacatgatgg tgtaacacta agacaaataa
     3361 agtcccactt tacagactat tctaaaattg accgtttgtt agaagaatat attaaccatg
     3421 gatatattct tcgccaaaat aaacgctaca gtcttaatct tccttttctg agttcgctgg
     3481 atggattagc attagatgat ttggttttca tagattctga tagtcaaatc tatcagttat
     3541 tacagaagcg aaaatttgtg acaaatttag ataaccaaac caatcacttg gtatttgtag
     3601 aagagacaga ttttgaaaga aacactttga ctctttctaa ttatttctat aaactaacca
     3661 acggttatcc actttcaaga gaacaaaaaa agctttatca acttttagga gatgttaatt
     3721 cggaatatgc tttaaaatat atgtcaagct ttatattgaa attccttcgc aaggatagtg
     3781 ttaagcaaaa acgtacagat atttttatcc aagctttaga attacttgga tatattagct
     3841 taaaccaaga tactacctat cgtttaaatg ctaaacttga tgttgaagct ttaaagattt
     3901 acttaacata aaaaagactg gaagtttgtc cagtcttttt tagtcaactt ttgtcatcaa
     3961 ccagagaaaa tagggggcac caattattga tacaaccaat ccagtgggta tacctgtacc
     4021 aactaagagg agacgaccta tagtatctga taccaatagg ataatagcac caataagaag
     4081 acagctaggt agtataattt tatggttatt tcctaatagt cgtctactta agtgcccagc
     4141 gagtaagcct ataaaagtaa tattaccaat taagacgacg ctaatagcag caaggctact
     4201 tgccagcatc aaagtatata ttctttcctt ttttaaatga agtcctagag cgagagcagt
     4261 ctgctcgttt aggttcataa tattaaggtg gcgactgcga ctatatgtta atccccataa
     4321 caaaatgaga atcgggataa aagtgataat ggtagtccag gaactaattg taatttttcc
     4381 actaagccat tcaacgatca tatccatttt ttgattatta gactgactaa taatagaaat
     4441 cataatgcct gaaatcattg tagatattcc gataccagtg acgattaatc gtgtagggct
     4501 aatattgtga tttcttctgt atgacataag atagacaaga aaaatagtta ccaaccctcc
     4561 aaacatagca aataggggta aaaagtataa tatagttgga ttgctaacat taaatgttcc
     4621 tatggttaga gcgattacta atccagctcc agcattgata ccaagaatac cggaatcagc
     4681 tagtggattt ttggttaacg tctgtaatag caacccagca attcctaaag aagctccccc
     4741 aaagatacag acaataatac gaggtaaacg aatgttagta atgataaaaa ggaaggcatc
     4801 atcagatttt ccacttatta gttttaaaac gttaataacg gaagtattag catagccaag
     4861 tgaaattgat actagtatta ataatagtag gataatgaca gatgatatta aaacaaaagg
     4921 agctttattt ttttgaatca tagatttttc ccttttctaa ttaaccatag gaaacatgga
     4981 agccctacga tactgattat agaactaatt ggtgtctctg aagggggatt tataatacga
     5041 gatgataagt ctacccagat catgaaagta gcccctgaaa atcctattaa aggtaataac
     5101 agacgataat ctttgggaat aaaaagctta ataaaatgtg gaataatcaa tccaatgaaa
     5161 gaaacggttc ctatcaacgc gactgatgaa gccgataaaa acaaaacaat aagtaataga
     5221 aacgcagtca ttaattgagt tttttgtccc aatgctttgg ccacagattc attaaggctc
     5281 aagatagtta attgatgtgc taggagttgt gaaataagta aacctaaaat aatgataggt
     5341 gctattatga ttagcatttt ccagttaatt tgggataaac cacctgcttg ccaaccgata
     5401 acagttcgat tgagtttaaa gtataatgta accacttgtc caacagacgt aaaaagtgtg
     5461 gagatcatcg ctccagctaa aatgagtcgt agttggtggt aacccttttg ctttgtataa
     5521 gagagtgtga agactaaaag acaagaaatg acagacccta gtaagcagac aatgagaatc
     5581 gtactatagt gcatgctccc taaaaaagca tatgcaacaa caagggctaa cccagatccg
     5641 gcgttaatac ctagtagtcc aggatcagca atggcgtttc gagtgactcc ctgcataata
     5701 gcacctgctt gtgacatagc tgcacctact aagatagctg caattatgcg tggtagtcta
     5761 atgtcaaaaa taacatcctg agcattagag ttttttaagg gattttttaa aacagttatt
     5821 aaatcactag tcttgaaatt gatagcacca aatcgtaagc tggcgtaaca accaataaga
     5881 aatagtataa gtgttatgaa aaaaacaagc caaaaatgat ttgacttatt tttttgaacg
     5941 agtttttttg aagctttaaa cataagagga gctttctttt aatttgtatt ttctttgata
     6001 gcctttgtaa atgattttaa ttgagcttct aaagatagag ggtcagagaa ataaaacacg
     6061 tcgtagttac tttctatgat gtgccctttt ttgacagctg gtaaattctt ccagacatca
     6121 ctttctttaa gtgatgaagc tgctttttta gtcgttttgt ttatattaac aagggcataa
     6181 tctccaacgt aatcaccgat tgcttcttgc gaaacggtaa accacccttt tttaaagaca
     6241 tcttttttga ctttttctgg ggcagcataa cctagtgaat catagattag ttctccaccg
     6301 cgtccaaaat tattaccata taaatagata tttttatcat aaaaatccat aatagtaaaa
     6361 gtagtgttag gctttaagat atggtgtaaa tcttttttgg cagcgagagt tttagttttc
     6421 cattggctaa cccactgatt agcttctttt tctttaccga atactttccc caaggctggc
     6481 atcatatcta aataattttg tgcaccatat ttaataacta aagttggtgc aatttttttc
     6541 agagtattga tgtttggatc ttgatcgaaa accatgatta aatcaggttt ttgtgcggca
     6601 atagcttctg tatcatcagc agttaatttt ttagcttctt tcagttgctt accaaaaacg
     6661 gggctatctt tttctaagtc taaactgtaa cttgaaacat taacacctag ttttaataaa
     6721 tacccagtgt aagaatatgt aaaattaatt acttttttcg gattttcagg aatttttcca
     6781 taataggtga agccttcaat tttaggcatt ttagaaatag ttgttttagt gctttcttgt
     6841 ttagtttgtt gtccgcaaga taccaaaaag aaggtcagta gtgtgaggac aataattcca
     6901 atttttttca taagataaca tctcctaaat aatattaatt ttgatcagtt gataattgat
     6961 aagataacaa aacaggttgt ttactaatag ggtcctgaat gatatgagca tcaatcttaa
     7021 aaatatcttt gataatatct ttagtcatga tctgactagg actaccttca tagataattt
     7081 taccggtttt cattgcaact agataatcag aatagcgcgc agacaggttt aggtcgtgta
     7141 ggaccataat aattgttttt tgcgtttcgt tattaagttt tttgagcaat tccaagactt
     7201 ctaattgatg attcatgtca agataagttg taggttcgtc taaaaagata gtatctgtat
     7261 cttgtgctaa agccatagca atccatactt tttgcctctg acctcctgaa aggtcatcaa
     7321 catctctatt agcaaattgc gctacgttag tcatttccat cgcccaatgg attttactgc
     7381 gatcgtcatt tgtcaaattg ccaaggtatt tttggtgtgg gaaccgtcca taagatatta
     7441 gttcataaac tgttatccct tcagttgctt ctaacacctg tgggagaagt gcaatcttct
     7501 tagcaatttc ctttgtgggt aaatgtgcaa tatcatgacc atcaatagtg atagtaccat
     7561 cttttatttt atggatgcgt gtgagagcct tcaaaagagt agactttcca catccattgg
     7621 caccaataat cgttgttatt ttttggttaa gtatactgag ggaaagatta ttgatgattt
     7681 ctttctgatc gtaagatacg ataatgtttt ctgcttttat gtgagacata gagaaccttt
     7741 ctagcaaaaa attaagttca ttataacata gaaaaaagac ttacaaaagc cttttttagc
     7801 attaattaag aaaaatgtga acaaaatgat aattatttgg aatgattcaa aatagtatca
     7861 ataatcatta ttgtttagct ttttttatcg tttaaggtat tttttcgcaa cttctaaatc
     7921 tccagagtaa ttgtcacgat ttcctttgat aatttgataa gcatctgctc ctttaccagc
     7981 gataataatt gcatctagtt tgcaattagt aagagtactg gcattagcaa ttgcttcttc
     8041 tcgatcaatg atgatagtaa ccggacgatt aatatggctt gctatttctt gtgagataac
     8101 taagggatct tcaaaattag gatcatctgc agtgagtatc acttgtagat taggatgttg
     8161 attaatgaca tctccaaagt ctttacgacg gctttgacct ttattaccag gagctccgag
     8221 tactaatata atatcccctt tttggtgttc ctcaacaaca gctaataatt tcttcaaact
     8281 gtctccgttg tgggcatagt caacgaaaat ctttgcgccg ttggtttgtg ttaggacttc
     8341 catgcggcca gggacggtag tttgtgcaat tccgtttttt atatcttcaa ttgaaacacc
     8401 aagtcggaga caagcaagtc cagctgcgat agcattttct tgattgaatt ttccaatcag
     8461 cttgatatcg taggtgttct ccaactttcc tttgacatgg aaagaaaagg cctttgattc
     8521 tgtgataaca ttttcagaat aatctccata gaaatcatga ggaatatatt cgacctgttc
     8581 tttgacaata ttaaaatgat ccatctgact atttacaaca acagcattgc tattctccat
     8641 caagagccgt ttgtggaaaa aataatcttc gaatgtaggg tgctcgatag gaccaatatg
     8701 atcgggacta atatttaaga aaacgccaac gtcaaaggtt aaaccataaa cacgtttggt
     8761 taggtatgct tggcttgata cttccataat taagtgtgtc atttgattct caacggctgt
     8821 agccatcatc cgaaataaat caagactctc gggggttgtg agatgtgatt taaagaaaga
     8881 tttaccatct aatgttgtgt tcatagttga gagcatagca ggacgatggt ttacttttaa
     8941 catatgataa gcaaagtaag ccgcagttgt ttttcctttt gttccagtaa aagctagaag
     9001 tttaagtttg ttttgtgggt tattatagaa agacatagat atcaaactca tggctttttt
     9061 gatatcattg actaatatca ctggaatatc ggcgccgtaa tctatctcag aaacgtaaaa
     9121 agaaaggcct gctgtgattg cagaatctag atattctttt ttaaaagtag cacccttagc
     9181 gaaaaataaa gtatcagatg aaatttgacg actatcatat gataaggcat taaaagtta
//
LOCUS       DATRSD010000076         8523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRSD010000076 DATRSD010000000
VERSION     DATRSD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.077"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            141..1490
                     /locus_tag="VBD85_001622"
     CDS             141..1490
                     /locus_tag="VBD85_001622"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO7134165.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            1812..2339
                     /locus_tag="VBD85_001623"
     CDS             1812..2339
                     /locus_tag="VBD85_001623"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO7134166.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDCKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            2330..3007
                     /locus_tag="VBD85_001624"
     CDS             2330..3007
                     /locus_tag="VBD85_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO7134167.1"
                     /translation="MSMKKFAKEYPTTVLLVSLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAITGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            3139..4182
                     /locus_tag="VBD85_001625"
     CDS             3139..4182
                     /locus_tag="VBD85_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7134168.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGLHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EDIVSGKIQVPMK"
     gene            complement(4273..5172)
                     /gene="galU"
                     /locus_tag="VBD85_001626"
     CDS             complement(4273..5172)
                     /gene="galU"
                     /locus_tag="VBD85_001626"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO7134169.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(5209..6225)
                     /locus_tag="VBD85_001627"
     CDS             complement(5209..6225)
                     /locus_tag="VBD85_001627"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7134170.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVANLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            6395..6724
                     /gene="rnpA"
                     /locus_tag="VBD85_001628"
     CDS             6395..6724
                     /gene="rnpA"
                     /locus_tag="VBD85_001628"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO7134171.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAIKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            6737..7552
                     /locus_tag="VBD85_001629"
     CDS             6737..7552
                     /locus_tag="VBD85_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7134172.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPLIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            7560..8381
                     /locus_tag="VBD85_001630"
     CDS             7560..8381
                     /locus_tag="VBD85_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO7134173.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVITSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRTETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2851 a         1348 c         1620 g         2704 t
ORIGIN      
        1 agtgtttttg gaaataatta tgaaagtttc ataaaaattg gtagcgtttc aaaaaatatg
       61 aaaatacagt taaaaaacct tgaaaaaaaa ctcacaaaag agtaagatga tagtgaaaca
      121 taattgttag gagaaataaa atgacacata ttacatttga ctattcaaaa gtcttgggtc
      181 aatttgtagg cgaacacgag ttagactacc tacaaccaca agtaagtgca gcagatgctt
      241 tccttcgtca agggactggt cctggctcag attttctcgg atggatggac ctcccagaaa
      301 actatgacaa agaagaattt tctcgcattc aaaaagccgc tgaaaagatt aaatcagata
      361 gcgaagtact cgtggttatt ggtattggtg gttcgtacct tggcgcaaaa gcagcaattg
      421 actttttgaa taatcatttt gctaatttgc aaaccgcaga agaacgtaaa gcgcctcaga
      481 ttctttatgc tggaaattct atttcatcta cttaccttgc cgatttagtt gaatacgtcc
      541 aagataaaga attctcagta aatgtcattt caaaatcagg tacaacaact gaaccagcga
      601 ttgctttccg tgtatttaaa gaacttctag ttaaaaagta cggtcaagaa gaagctaata
      661 aacgtatcta cgctacaact gataaagtta aaggtgccgt aaaagttgaa gcagatgcta
      721 acaattggga aacatttgta gttcctgata atgttggtgg tcgtttctca gtactgacag
      781 ccgtaggtct tcttccaatt gctgcttcag gagcagatat tactgctctt atggaaggcg
      841 caaatgctgc tcgtaaagat ttatcttcag ataaaatctc tgaaaacatt gcttatcagt
      901 acgctgcagt tcgtaatgtt ctttatcgca aaggttatat cacagagatt ttagctaact
      961 acgaaccatc attacaatac tttggtgaat ggtggaaaca attagcaggt gaatcagaag
     1021 gtaaagatca aaaaggtatc tatcctacat cagcaaactt ctcaactgat cttcactcac
     1081 ttggtcaatt tattcaagaa ggttaccgta acctctttga gacagttgtt cgcgttgaga
     1141 aacctcgtaa aaatgtgact attcctgaat taaccgaaga tttagatggt cttggttatc
     1201 ttcaaggaaa agatgtagat tttgttaata aaaaagcaac agatggtgta ctccttgctc
     1261 atacagatgg tggggttcca aatatgtttg taacgcttcc tacacaagac gcttacaccc
     1321 ttggttacac tatttacttc tttgagttag caattggcct ttcaggttat cttaactcag
     1381 taaatccatt tgatcaacca ggggtagaag catataaacg taatatgttt gctcttctag
     1441 gtaaacctgg attcgaagag cttagcgctg aattgaatgc acgtctttaa aaatatctaa
     1501 ctaaaaagtt cctcaaagag aggaactttt tagttttgtt gtaggataac taaaaaccca
     1561 ctagcaccgg tagcgatagt gggttggaaa aagggttgag aaaagttatt aacttttctc
     1621 aggagattta tgaaaaagga aaataattta ggataatatt ttcctaacgg ctaggataaa
     1681 ccgcttattt taggatgata ctagtataca gtagaaacct taaataaacc ttaaaaattc
     1741 atactttatc attttatttt cccctatact atttttgtat aaatggtcaa tattaatggt
     1801 atcatagata tatggaaaag aaattattaa gaaaagaagt tttgataacc ttaaaatctc
     1861 aacctcaagc ttacaaatca gaagttgact gtaaactttt agaggctttt ataaaaacta
     1921 aagcttatca aaatagttgt gtaattgcta cttacctatc ttttgactac gaatacaata
     1981 cacaacttct tattaaacaa gctttatgtg atggcaaacg tgtccttgtt cccaaaactt
     2041 atccaaaagg taaaatgatt tttgtggact atcaaaaaga taacttgagg gcaacaccgt
     2101 ttggcttatt agaacctgtt aatgatagag ctgttgaaaa agctagtata gacttaattc
     2161 atgtgcccgg acttatcttt aacaataaag gttttcgtat aggttatggt gcaggttatt
     2221 ttgatcgcta tttgagtgat tttgaaggtg atactattag tactatttat aggtgtcaga
     2281 gacaggattt tgtagaagag aagcatgatg tagcggttaa ggaggtatta tgtctatgaa
     2341 gaagttcgct aaagagtatc cgacgacagt actcttggtc agtctgacaa cccttgtttt
     2401 tttattgatg caactaactt atggaagtca agcggagtct agccaagtta tttttcaatt
     2461 tggtggaata caaggtgatt atctaaaagc atatccaaca aatctatgga gactaataag
     2521 tcctatcttt gttcatattg gttgggaaca ttttttatta aatggtttag ctctgtattt
     2581 tgtaggacaa atgggggaaa gtatttgggg aagtttgaga tttctaattc tctatatttt
     2641 atctggctta atgggtaata tttttacgct attttttact ccacacgtgg tggcagcagg
     2701 agcctcaacg tctttatttg gtgttttttc tgctatagca atcacaggtt attttggaaa
     2761 aaatccttat ttaaaacaag ttggaaaatc atatcaagtg atgatcttgt taaatttatt
     2821 ttttaatatc ttcacacccg gggtgagttt agcaggtcat gttggcggtt tagttggtgg
     2881 agtattggtg gctatttttt tgacaaaaca aaatggttct ttattattta aaacttggca
     2941 aagtatttta gctttaatga tattcattat tgtatcgatc agcttaatag gtctatcgtt
     3001 ggtataattc tacggctata gtctctaaaa ccaaactttc tctgggaaaa atagaataaa
     3061 gtttgctaaa tgattttttt atgttatgat agaaatctgt ttttatgtga ctataactta
     3121 ttaggaggtt cattctcggt gaacagaaaa attattggct taactctact atctttatct
     3181 gtactaacat taacagcatg tggtaatcgt tcagataagt ctgcaaataa atcagatatc
     3241 aaagttgcga tggtaactaa tcaaggtgga gtagacgata aatcgtttaa tcaatcagct
     3301 tgggaagggc tacaaaagtg ggggaaaaag aaggggttga caaaaggtaa tggctttgac
     3361 tatttccagt catctaatga atcagatcat gctaataacc ttgatacggc agcctctagt
     3421 ggctataatt taatttttgg tattggattt ggtcttcatg atactattga aaaagtatca
     3481 gaaaataata aagatgttaa atacgttatt gtagatgata tcattaaagg taaagaaaat
     3541 gtagctagtg ttacttttgc agacaatgaa gctgcttact tagctggagt tgctgcagct
     3601 aagacaacta agacaaaaac agttggtttt atcggaggga tggaaggcgt cgttgttaag
     3661 cgatttgaag caggatttaa ggctggagta aaatcaattg atccggctat taaagttgcg
     3721 gtatcttatg caggctcatt tacagatgca gctaaaggta aaacgattgc tgccacacag
     3781 tatgcaacag gtgttgatgt tatttaccag gctgctggtg gtacaggagc aggaattttt
     3841 agtgaggcta aaactgaaaa tgagacccgc aaggaatcta acaaagtctg ggtaattggt
     3901 gttgatagag atcaaagtca agaaggcaat tatgtttcaa aggatggtaa gaaagcaaac
     3961 tttgttctgg catctactat aaaagaggtg gggaaatctc tccaatctgt agcagaatta
     4021 acagagaaaa aacaatatcc aggagggaaa gtaacagttt ttggtctaaa ggatagtggt
     4081 gtagatatta aagagcatca attgtctagt gagggatctg tcgctgtcaa aaaagctaaa
     4141 gaggatattg tttcagggaa aatacaagtt cctatgaaat gataaaagtg tggaggaaca
     4201 ataagttctt catcaagtac aataaaaaag tttcctattt aggaaacttt tttattgtac
     4261 ttgactcata ttttacttct tagatgtttt ttctagagat tttcctaaat caatgatata
     4321 tttcttcaag tcatctttaa cttgtgggtg ttgaagagca taatcaatag atgttttcat
     4381 aaaaccaaac ttatcaccaa cgtcataacg gtctcctgta aattttctag caaaaacacg
     4441 ttgcgtttta tttaaagtat cgatagcatc ggttagttga atttcatttc ctgcaccagg
     4501 tttttgagtt tctaaaatat taaaaatttc tggagttaac aagtagcgac caatgattgc
     4561 caaattactt ggagcctctt cgggagaagg tttttcaacg aaagtgttaa cgctatatag
     4621 accattaact ccttctcctt gtggagcgat aacaccgtaa gcagaaacat cttcatgtgg
     4681 aacttccata acagcaattg ttgatgcatg agtagcttca aagtcattaa ttaattgttt
     4741 ggtgagagga ataactttat tattggtaat atccattaga tcatcaccta gcataacaac
     4801 aaaaggttca ttaccaacga aggcttttgc ttgtagcaca gcatctccaa gcccacgagg
     4861 atgactttgt cgaataaaat ggagtcgaat gccagttgtt tcatcaacaa gtttgagaag
     4921 ttcgtttttc cctttctctt ttaggttata ttctaattca aaatttgaat caaagtggtc
     4981 ttctatagaa cgttttgatt taccagttac aactaaaata tcttcaattc cagattttag
     5041 tgcttcttca acaataaatt gaatagttgg tttatcaacg attggaagca tttctttagc
     5101 taaagcttta gtagcaggta aaaaacgtgt tcctaaacca gcggcaggga taactgcttt
     5161 tctgactttc atatatagct cctttaaata gtattaatat aaacgatatt agtgccattc
     5221 attctctgaa cgaaattcat tggacatcat atctaaaatg ctatctttga tattagcccc
     5281 ttcatatatt gatttataaa tagcttctgt aatgggcatg taaacattta agttttgggc
     5341 gatttcgtaa gcaaccttag ttgtcgagat accttcaata accatcccca tatttttttc
     5401 aatatcttca agtttctcac cacgtcctaa agcatctccc gctctccagt tgcgagaatg
     5461 aacagatgta ccagtcacaa ttaaatctcc aactccggat aaaccactga aagtgagagg
     5521 gtcggctcct aattgtacac caaggcgtgt tatctcagct aaaccacgcg taataatagc
     5581 tgctttagca ttatcaccat aaccaagacc gtgtagtgct ccagcaccaa cagcaataat
     5641 attttttaga gcaccagctg tttccactcc tacgacatca gtgttagtat aaagtctaaa
     5701 atagtgatta ctaaacaatt tttggacata cttagctgct tcaatatctt ttgaagcagc
     5761 agtaatcaaa gtaatatcac gaacgatggc ttcttcagcg tgactgggac ctgaaacaac
     5821 aacgatatca ctacgatatt gttcagagat ttcttcttct aaaattgtag aaagtcgttc
     5881 atgagtgcca ggttctagtc ccttagaagc atgcataata acaactttat gctttaaaag
     5941 attagccact tgtttagcaa caagtcttgt aactttagtt gggacgacga atagaatgct
     6001 gtcaacatta ttaatcgctt cctcaagatt agtgtaggct tttatcttgg aatctaaggt
     6061 aatatctttg aagtaacgtt gattagtatg gttagtatta atctcttcta tttgttcaac
     6121 cacgtttccc cataagcgta cctcatgtcc attgtcattt aatacttgtg caagagcagt
     6181 cccccatgaa ccaggtccaa gaacggcaat tttttgtttt gtcatcttat tccttctttc
     6241 taataataag gcatatagcg ctaacattat atcatactct aatacacttt gtcttgttta
     6301 gtctgttgtc gatgatagta acattagaaa agaattgagt cttttaggtt atttatggta
     6361 taataaaacc aatttagata aatggagcac tattttgaaa aaaacctatc gggtaaaaag
     6421 tgataaggat tttcaaatga ttttcagcag aggtaaaaat gttgctaatc gaaaatttgt
     6481 tatctactat ttagaaaaag aacaaaaaca ctttcgtgtg ggaatctctg ttagtaagaa
     6541 attaggaaat gctgttgttc gtaacgctat taaaagaaaa attaggcatg ttttactctc
     6601 tcagaaaaca gccctgcaag attatgattt tgtcgtgatt gcgcgtaaag gtgtagagga
     6661 attggactat caagcattag aaaaaaatct tatacacgtt ttaaaaatag caggactcat
     6721 ctagaaaggt ataaaattga agaaaaaact taaaacattt agcttaatat tgctgacagg
     6781 ttctttattg gttgcttgcg gacgaggaga agtatctagt cattcagcta cattatggga
     6841 gcaaatagtt tatgcatttg ctaaatcaat acaatggctc tcctttaacc attcaattgg
     6901 tttgggaata atcttattca ctctaataat cagagcgatt atgatgccgc tttataatat
     6961 gcaaatgaaa tccagccaaa agatgcagga aattcagcca aggctcaagg aattacaaaa
     7021 aaaatatcca gggaaagatc ctgatagccg tttaaagcta aatgatgaaa tgcaatctat
     7081 gtataaagct gagggagtta acccatatgc aagtgtatta cctcttctaa ttcagttacc
     7141 tgtcctatgg gctcttttcc aagctttgac tagggtttct ttcttaaaag taggaacatt
     7201 tttgagccta gaattatcac agccggaccc atattatatt ttacctgtac tggcggcctt
     7261 atttaccttt ttatcaactt ggttaactaa caaagcagct gttgaaaaaa atattgcttt
     7321 aactcttatg acttatgtta tgccgcttat catattggta acaagtttta actttgctag
     7381 tggtgttgtt ttatactgga cggtgtctaa tgcttttcaa gtatttcaaa ttctattatt
     7441 gaacaaccca tacaagatta taaaggttcg agaagaagca gtaagagtgg cacacgaaaa
     7501 agaacaaaga gttaaacgtg ctaagagaaa agcaagtaag aaaagaaagt aggaaaaaca
     7561 tggtattatt tacaggagcg actgtagaag aagctattga aaaaggacta caagagttaa
     7621 acatttcacg attgagggca catataaaag ttgtatcacg tgagaaaaaa ggttttttgg
     7681 gctttggtaa aaagccagca aaagttgaaa tagaaggtat caccgacgaa gtaactgata
     7741 ttaatgaatc ggtagcgtta aaaaatatta agaatgtacc gtctagcgta gatgtggtgg
     7801 aagaatatat agaggaggtt gatgaaacac ttgaaaaaga agacgtatca caaccagaac
     7861 ttccaaaaat tgatgataaa aacgttgtaa ttacaagtga agctattgaa aaaattgacc
     7921 tacttcctaa tattgaagta gcagcagctc aggtgactaa atacgttgaa aatatcatct
     7981 atgagatgga tttagatgct acaatagaaa ctacgacaag taagcgacaa ataaatctcc
     8041 aaatagaaac gccagaagct ggacgcatca ttggttatca tgggaaagta ttaaaatcgt
     8101 tgcagttgtt agcacaaaat tatcttcatg accgcttttc taagagtttt tcagtatcca
     8161 ttaatgtcca tgactacgtt gagcatcgta cagaaacttt aattgatttt tctaaaaaaa
     8221 tagctagacg tgttttagag actaatgaac cttaccatat ggatcctatg agtaacagtg
     8281 agcgaaaaac tgttcataaa acaattgcaa ctatagaggg tgttgaaagt tattctgaag
     8341 gtaacgatcc taatagattt gtagtagtta ctaagaagta aaaagaggtc tcacctcttt
     8401 ttttcttgcg tccgatttac attttcagaa aatgtagaaa aagtatgaaa aaagagttga
     8461 caaagtaaga gatgatgtag taaactaaat aagttgtctc gagagagcag tttctattag
     8521 cgc
//
LOCUS       DATRSD010000077         7980 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRSD010000077 DATRSD010000000
VERSION     DATRSD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.078"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(207..356)
                     /gene="rpmG"
                     /locus_tag="VBD85_001631"
     CDS             complement(207..356)
                     /gene="rpmG"
                     /locus_tag="VBD85_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7134174.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(372..554)
                     /gene="rpmF"
                     /locus_tag="VBD85_001632"
     CDS             complement(372..554)
                     /gene="rpmF"
                     /locus_tag="VBD85_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO7134175.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            774..2054
                     /locus_tag="VBD85_001633"
     CDS             774..2054
                     /locus_tag="VBD85_001633"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO7134176.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPATDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILGKQGIPLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     REVSLKNNQTRQEVKVSFENIKTGFSSVLKQLGL"
     gene            2147..3898
                     /gene="aspS"
                     /locus_tag="VBD85_001634"
     CDS             2147..3898
                     /gene="aspS"
                     /locus_tag="VBD85_001634"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO7134177.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVSASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEVVKEVDFKVFSEASVVKAIVVKNKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYADNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            3888..4832
                     /locus_tag="VBD85_001635"
     CDS             3888..4832
                     /locus_tag="VBD85_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7134178.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            4940..5812
                     /locus_tag="VBD85_001636"
     CDS             4940..5812
                     /locus_tag="VBD85_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7134179.1"
                     /translation="MLKLDLKTKIKEAILIALGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(5839..6147)
                     /locus_tag="VBD85_001637"
     CDS             complement(5839..6147)
                     /locus_tag="VBD85_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7134180.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(6235..7926)
                     /locus_tag="VBD85_001638"
     CDS             complement(6235..7926)
                     /locus_tag="VBD85_001638"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO7134181.1"
                     /translation="MDTKHLIASEIQKVVPDMEQSTILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAETDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMEEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
BASE COUNT         2474 a         1301 c         1581 g         2624 t
ORIGIN      
        1 ataagttagt gtaccaaata ttaaaaagcc ctgccatcga aatgatagca gggcttaact
       61 tcaatatcca gatgatatat ttatctaaaa aaggtagagt aaaaggtaga gtttttattg
      121 atttgtagtg cgatataatg agtttcaaaa aatcaaaaat cgcttaaaat caatattttg
      181 acgtctattg acgtgtactg aaacccttac ttaacctcag taaaaacaac gtgtttacga
      241 agttttggtg agtatttttt caattgaaga cggtctggag tgttacgttt gttttttgaa
      301 gtaaggtaca agcgttcacc agattcttta tgttcaagtg taatatttac gcgcatggta
      361 tctcccttct attatttagc ttcgttagct ttagcgattt tacgtccttt gtagtatcct
      421 ttgagtgata cacggtgtga acgtgagtaa tctccagtag tttcgtcgaa ttgtacagat
      481 ggagctgtca atttgtagtg tgtacgacgt ttgtttttct tcgcttttga agtgtgacgt
      541 gcaggtactg ccattttctt attcttcctt tcgatgtgta agttttgatt ttcatcaact
      601 tcactatgat aacatatctt ttttgtaaag taaagttact tgacaaaact ttttattttt
      661 acaatttcat ggtgaaaata ctatcaaact aaagcaaatg atatatgctt gtttaaaata
      721 taagaaaaaa tggtagaatg tttaaagaga aaactaatcg aagaggtaaa cttatgaaac
      781 ttcaaaaacc aaaagggact caggatatct tacctggaga atcagcaaag tggcaatacg
      841 ttgaaaatgt tatacgcaat ctctttaaac aatatcatta tgatgaaatt cgtacaccaa
      901 tgtttgaaca ttacgaggtt attagtcgct cagttgggga tacaacggat atcgttacaa
      961 aagagatgta tgattttcat gataaaggag atcgtcacat tacgcttcgt ccagaaggaa
     1021 cagcacctgt tgtacgttct tatgttgaaa ataaactatt tgcaccagaa gttcaaaaac
     1081 caaccaaaat gtattatatt ggatcaatgt tccgatatga gcgcccacag gctggtcgtt
     1141 tacgagaatt tcatcaagta ggcgttgagt gctttggatc aaataatcca gcaactgatg
     1201 ttgaaacaat cgcaatggga catcatctat ttgaagattt aggtatcaaa aatgttaaac
     1261 ttcatcttaa tagtttaggg agtccagaaa gtcgtcaagc ctatcgccaa gctcttattg
     1321 attacttaac acccatacgt gaacaattgt ctaaggatag tcaacgtcgt ttgaatgaaa
     1381 atccattgcg cgtactagac tctaaggagc cagaagacaa actagcagtt gaaaatgctc
     1441 cttctatttt agattattta gatgagtcaa gccaagccca ctttgatgct gtgtgtcata
     1501 tgcttgatgc tctaaatatt ccatacatca ttgatactaa tatggttcgt ggtcttgatt
     1561 attataatca tactattttt gagtttatca ctgagattga agataatgaa ttaacgatct
     1621 gtgctggagg ccgttatgac ggcttagtat cctattttgg tggaccagaa actccagcat
     1681 ttggttttgg attaggttta gaacgcttgc tcttaatatt aggtaagcaa gggattcctt
     1741 taccaataga aaatactata gatctgtata tagctgtatt aggatcagag gctaatctag
     1801 ctgccttaga tttggcacaa tcaatacgtc atcaaggttt taaagtagaa cgtgattatt
     1861 tgggtcgtaa aatcaaagca caatttaagt ctgcagatac ttttaatgct aaagtgatta
     1921 tgacattagg tagcagtgaa gtagattcga gagaggtcag tttaaaaaat aatcaaacac
     1981 gtcaagaagt taaagttagt tttgaaaaca ttaaaacagg tttttcatct gtgcttaaac
     2041 aattaggatt gtaaaacagt tcctaaaaac tctccttaat gggtggggtt tttaggtgct
     2101 ttttgttata ataggttgga atgaatattt taaatggaga aaacagatga aacgtagtat
     2161 gtatgctggg cgtgttcgta gtgagcacat aggaacatcg attactttaa aaggatgggt
     2221 tggtcgtcgt cgtgaccttg gaggtcttat ttttattgat cttcgcgatc gcgagggaat
     2281 catgcaatta gttattaatc ctgaagaagt atcagcctca gttatggcaa ccgcagaaag
     2341 tctccgtagt gaatttgtta tagaggttag cggtgtagtg acagcacgag aacaagctaa
     2401 tgataattta ccaacaggag aagttgaatt aaaagtgcaa gaattatcgg ttcttaatac
     2461 ctcgaaaaca acaccatttg aaattaaaga tggtattgag gcaaatgatg atacacgcat
     2521 gcgttaccgt tacttggatc ttcgtcgtcc ggaaatgctt gaaaatttca aattacgtgc
     2581 gaaagtaaca cactcaatcc gtaattacct tgacaactta gagtttattg atgtcgaaac
     2641 tccgatgcta acaaaatcaa ctccagaggg ggcacgagac tacttggttc catcacgtgt
     2701 caaccaagga catttttatg cccttccaca aagccctcaa attacaaaac agctattgat
     2761 gaatgcggga tttgatcgtt attatcaaat tgtcaaatgt ttccgtgatg aggatttacg
     2821 tggagatcgt caacccgagt ttacacaagt tgacttagag acatcattcc ttagtgatca
     2881 agaaattcaa gacattgttg agggtatgat tgctaaagta atgaaagaca ctaaaggttt
     2941 agaggtttct ttaccattcc caaggatggc atatgatgat gcaatgaata actacgggtc
     3001 tgataagcct gatacaaggt ttgatatgct tttacaagac ttgacagaag ttgtaaaaga
     3061 agttgacttc aaggtattct cagaagcttc agtagttaaa gcgattgttg tcaaaaataa
     3121 ggcggacaag tactctcgaa aaaatattga taaattaaca gaaattgcaa aacaatacgg
     3181 tgccaaaggt ttagcatggc taaaatatgc agataatact atttcaggtc cggttgctaa
     3241 gttcttgaca gctattgaag gtaggttgac agaagcttta cagctagaga ataatgactt
     3301 aattctattt gtagcagata gtcttgaagt agctaacgag actttaggag cgcttcgtac
     3361 acgcattgca aaagaacttg aactcattga ttattctaaa tttaatttcc tatggattgt
     3421 tgattggcca atgtttgagt ggtcagaaga agagggacga tatatgtcgg ctcatcatcc
     3481 attcaccttg ccaacagcgg agacagcgca tgagttggaa ggggatttgg ctaaagtacg
     3541 tgcagttgcc tatgatattg ttttgaatgg atatgagtta ggcggtggta gcctacgtat
     3601 taaccaaaaa gatacacaag agcgtatgtt caaggcttta ggatttagcg ctgaatcagc
     3661 tcaggaacag tttggtttct tacttgaagc tatggattac ggcttcccac cacatggtgg
     3721 attggcaata ggccttgacc gctttgttat gttgttagca ggcaaggaca atattcgaga
     3781 agttattgca ttccctaaaa acaataaggc tagtgatcca atgactcaag ctcctagtct
     3841 cgtttcagaa caacaattag aagaattatc acttactgta gaaagttatg aaaactagac
     3901 gaaccccttt agaaaaaaag gtaaaatata ttatcagtgt atgggctaaa aaatttggtc
     3961 tcttacacac tttaaaaagt atctcacgag aaaaatatgc agagaaaatt tcggcttcat
     4021 tactgtatgg tattctatcc agtgttgctg tcaatttctt ttttcaacct ggacatgttt
     4081 attcaagtgg agcaactggt ttagctcagg ttatttcagc tgtcagtaaa cactggtttt
     4141 cttttgaaat accagttgct ttagcttttt atgctattaa tatccctttg ctgatattat
     4201 cttggcgtaa gattggacat aaatttacta tttttacgtt tatcacagta actgtaagct
     4261 ctatcttcat tcaattaatg cctcaaatca cccttacaac tgatccctta atcaatgcta
     4321 tctttggtgg cttaattatg ggagctggtg ttggttttag ctttaagtct agaatttcta
     4381 gtgggggaac agatattatc agtttgacaa ttagaaagaa aacaggcaga gatgtaggta
     4441 gtatttcttt tattattaat ggtattattt tattatttgc aggtctttta tttggttgga
     4501 agtacgcttt gtactcgatg gtgacaattt ttgtatcaag tagagttaca gatgccattt
     4561 ttacaaagca aaaaaagatg caagcgatga ttgttaccag taagcctgat tgcgtcatta
     4621 aacgtatcca tcgtgattta catcgtggtg taacctgtat caatgatgct gaaggtactt
     4681 ataatcatga gaaaaaagca gttttaataa ctatattaac acgtgaagaa tttagtgatt
     4741 ttaaatattt aatgctaaaa gcagatccaa aagcctttgt ttccgttgca gaaaatgttc
     4801 atattattgg acgattcgtt gatgatgatt aactgtatat atgacttttg gtcaactgcc
     4861 actaaggaaa cattatgatg ttattaagct aagttgttta aacaacttta gcttttttga
     4921 ataaggaagg attaagccta tgttgaaact ggatttaaaa acaaagataa aagaagctat
     4981 tttaattgcc ttgggtgtgg ctctctatac tttcggattt gtcaaattta atatggctaa
     5041 tcatttggct gaaggaggta tttccggtgt caccttgatt atccatgcac ttttcggagt
     5101 taatccagca ctttcatcac ttctccttaa tatcccgtta tttatattag gggctaggat
     5161 tttagggaaa aaatccttat tgttaactat atatggtaca gtgttaatgt cttttttcat
     5221 gtggttttgg caacaaatcc ctgtaacagt ccctttgaaa aacgatatga tgttagttgc
     5281 agtagctgct ggaattttag caggaactgg tagtggttta gtatttagat atggtgcaac
     5341 tacaggtggt gcagatatta ttggtcgtat tgtggaggaa aaatctggaa taaaattagg
     5401 gcaaacctta ttgtttattg atgctatagt tctaacgtct tcacttgttt atattaattt
     5461 acaacagatg ctatatacgt tagtagctag ctttgtattt agtcaagtgt taacaaatgt
     5521 tgaaaatggg ggctacactg ttcgtggaat gatcattatt accaaagaat cagaatctgc
     5581 agcagcaacc attttgcatg aaattaatag gggtgtaact tttttacggg gccaaggtgc
     5641 ctactcaggt agagagcatg atgtcttgta cgtagctctt aacccaagtg aagtgcgtga
     5701 tgtgaaagaa atcatggctg atttagaccc agatgctttt atttctgtta taaatgtaga
     5761 tgaagttatt agctcagatt ttaaaattcg acgaagaaat tacgataaat aaaaatacag
     5821 gctgtctggc ctgtattttt attttggtcc tccacttaag aaactaccaa gcataatagc
     5881 tgagatagca gttcccttgt aatggctagc ttttgttttt accaagcgac taagatcaaa
     5941 tatgctttta ggagcatcaa attgatgtgt taagacgtat tgattgataa gtgagtcaat
     6001 ttttgtagct aggagtgagt tcatcccctc tttttcaagt tgactagcga gctcgaaaat
     6061 agcttgacga aaataatcat ctgcttgtat tacttcttcg ctataaactt tactaagagc
     6121 atctaatatt tctttttctg atggcatggc attctcctgt aacttattgt gctattatta
     6181 taatataaaa aattaagact gtcataaatt tgacagtctt agtagtgtca tttcttacat
     6241 ttcattcgga gcatcaactc ctaggaggcg aagagattct tttaatactg tagcagttgc
     6301 atagcaaagt gctagacgac tactgatttc agcgtcttca tctaaaatac gtgtgtgggc
     6361 gtaatattta ttgaagcact gagctaaatt gatagcgaat tttgcaataa tagagggctc
     6421 aaaattatct gccgcacgta caataatacg aggaaaatct tggatgagtt tgatgatttc
     6481 ccagctttca acatcgttga gactgtaatt atcgctatta gatgggttaa agttagcttt
     6541 acgtaagatt gattgaatac gagcatgtgc atattgtaca tatggcccag tttcaccttc
     6601 aaatgatacc attgcttcta agtcaaagtc ataaccattt gtacgatcag ttttcaaatc
     6661 gtaaaattta atagcaccta caccaactgc ttgagcaact ttatctttat ctgctaggtt
     6721 agggttttta gcttcaatct gagatgccgc acgattaata gcttctgcaa cagtaggttc
     6781 aaggagaatt acatttcctt tacgagttga taattttgct cctccttttg ttacaaggcc
     6841 aaaaggaaca tgagtcatat catcggacca atcgtagtcc atttctttaa gaacagcttt
     6901 tagttgttta aagtgtgcag actgttcgtt tcctacaacg tagattgatt ttgcaaaatc
     6961 gtaggtacgt ttacggtaaa gtgcagcagc taagtcgcgt gtaatgtaga gagttgctcc
     7021 atcggatttc ttaatgagag caggatgttc aataccgtat ttttctaggt taacaacttg
     7081 tgcaccttta gattcgacca ggaggttttt agattctagc agttcaagca cttcctccat
     7141 tttgtcgttg tagaatgcct cgccgttata gctatcaaat gtaacattca ttttatcgta
     7201 aagacggttg aattctaata atgactcgtc acggaaccat tgccataatt cagttgcttc
     7261 aggatcgtta gcttcaagtt tacgaaacca ctcacgtgcc tcttcatcaa cgctagggtc
     7321 tgtctcggct tcagcattaa tacggacata aagttttaaa agttcatcaa tgggatgagc
     7381 acggacagcc tcttcattgc cccatttttt ataagcaacg attaacattc caaattgttt
     7441 accccagtcg ccaaggtgat taattttaac aggatgatag ccaattttat caaaaatatt
     7501 agctaaacta tcaccaataa ctgttgaacg aaggtgtcca attgagaatg gcttagcaat
     7561 atttggacta gacatgtcaa tggcaacatt tcgtccttca ccaatatttt gagtagcata
     7621 ggcagaacca tcagataaga cttgttttag aacttgactt gagattgcgg ctttatcaag
     7681 gaaaaagtta acgtaaggtc caacagcttc caccttttca aattggtcgc ttttaatttg
     7741 ttcagcaatg tcactagcaa ttatttgagg tgctttgcgt agagttttag ccaaagagaa
     7801 tgctgggaaa gctaaatctc ccatgctcga attttttggg gtttctaata aagaaagaat
     7861 ggtcgattgt tccatatctg gaacaacttt ttgaatttca ctcgcaatta gatgttttgt
     7921 atccatggta tctcctattt taattagtaa tatagtcacc ctattttaac ataattatga
//
LOCUS       DATRSD010000078         7945 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRSD010000078 DATRSD010000000
VERSION     DATRSD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.079"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..298
                     /locus_tag="VBD85_001639"
     CDS             <1..298
                     /locus_tag="VBD85_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HEO7134182.1"
                     /translation="YICLDEPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFA
                     THYADNVVALKEGQVVTCGTVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            360..1388
                     /locus_tag="VBD85_001640"
     CDS             360..1388
                     /locus_tag="VBD85_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134183.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            1526..2368
                     /gene="dprA"
                     /locus_tag="VBD85_001641"
                     /pseudo
     CDS             1526..2368
                     /gene="dprA"
                     /locus_tag="VBD85_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            2463..4583
                     /gene="topA"
                     /locus_tag="VBD85_001642"
     CDS             2463..4583
                     /gene="topA"
                     /locus_tag="VBD85_001642"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO7134184.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            4720..5421
                     /locus_tag="VBD85_001643"
     CDS             4720..5421
                     /locus_tag="VBD85_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134185.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            5433..>7945
                     /locus_tag="VBD85_001644"
     CDS             5433..>7945
                     /locus_tag="VBD85_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134186.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSP"
BASE COUNT         2814 a         1325 c         1399 g         2407 t
ORIGIN      
        1 atatatttgc ttagatgagc cactcaacaa tttagatatg aaatatgctg ttcaaatgat
       61 ggatcttatc aaacgatatg cgtatgagtt taataaaact atagtgatta ttattcacga
      121 catcaatttt gcaactcact acgctgacaa tgttgtcgct ttaaaagaag gacaagtggt
      181 gacttgtggg actgtcgaag atgttatgca agaaaaaatc ctttctcatt tatttgatat
      241 gcctatccgt atcgaaactg ttgatggtaa accaattatc cttttttgta caacgtaaat
      301 aaagagattt atgtaggata acatttttag tttttatcta ttttaggaga ttttatttaa
      361 tgacaaaaaa acttattatt gctatattag cactatgcac tatcttaacc acttctcaag
      421 ctgttttagc taaagaaaaa tcacaaactg ttaccataaa aaacaactat tcggtctata
      481 ttaaaaaaga aaaaagagac aagccggata ataaaaagca aatcagcgag acacttaaag
      541 ttcctttaaa acccaaaaaa gtagttgttt ttgatatggg agctttggat actatcacag
      601 ctttaggagc tgaaaaatct gttattggta tcccgaaggc taaaaatgct ctaagtttat
      661 tgcccaataa cgtcaaatct gtttataaag ctaagagata ccaagacgta ggaagtctct
      721 tcgaaccaaa ctttgaagct attgctcgta tgcaacctga tgtggttttc ctaggagcac
      781 gtatggcttc tgttgataat attgaaaaat taaaggaggc tgcacctaaa gcagcattag
      841 tatatgctgg agtcgactca aaaaaagtat ttgacaaagg agttgctgag cgtgtcacaa
      901 tgttagggaa aatcttcgac caaaataaaa aggcaaaaac ctttaataaa gatatcgcac
      961 aagctgttct taaattgcag aaaactattg agaaaaaagg taaacctaca gctctatttg
     1021 taatggcaaa cagcggtgaa cttttaactc aatcaccttc tggtcgtttt ggttggattt
     1081 tctctgtagg tggatttaaa gcagtcaatg aaaatgaaaa actaagttca catggtactc
     1141 ccgtatctta tgaatacatc gctgaaaaaa atcctaacta tctctttgtt ttagatcgtg
     1201 gagcgactat tggacaagga gcttcatcaa aagaactttt taataacgat gttattaaag
     1261 caactgatgc tgtcaaaaac aaacgtgttc atgaggtaga tggaaaagat tggtatatca
     1321 attcaggcgg aagccgagta acactccgta tgattaaaga tgtacagaac tttgttgata
     1381 atcgttaatt ttttgtcaaa ggctaggaaa aatcctagtc ttattttttt gaaaaaatag
     1441 ctattatcaa tgcttcagag tatagattga tcttatcact ttattttgtc tattattttc
     1501 gaatatagtc aatggaggta accttatgaa tcattttgaa ctatttaagc ttaaaaaagc
     1561 tggattaaca aatcttaata tcaacaatat tatcaactat ctcaaaaaga atagtttaac
     1621 ttctctatct gttcgcaata tggccgtagt atcaaaatgt aaaaatccta ctttctttat
     1681 agaaaattat aaacagctag accttaaaaa acttcgacaa gaattcaaaa aatttccagt
     1741 tctatcgatt ttggattcta actatccttt agagttaaaa gaaatatata atccaccagt
     1801 tctacttttt tatcagggta atattgaact tctatctaaa cctaaattag ctgtagtggg
     1861 ggcaagacag gcatctcaga taggttgtca gtctgttaaa aagattatca aggagactaa
     1921 caatcaattt gttatcgtaa gtggtttagc gcgtggcatt gatacagcag cacatgttag
     1981 tgctttaaaa aatggcggca gtagtatagc tgttatcggg agtggtttag atgtttatta
     2041 tccaacggag aataagaaac ttcaagaata tatgtcatat aatcatctcg tattatcaga
     2101 atattttacc ggagaacaac ccttgaaatt tcatttcccc gaacgtaacc gtattattgc
     2161 agggctgtgt caaggtattg tggttgccga agctaagatg agatctggaa gtttaattac
     2221 ctgtgaaaga gcattagaag agggacgata agtttttgcc attcccggaa atattatcga
     2281 tggcaaatca gatggatgcc accatcttat ccaagaagga gctaaatgca ttatttcagg
     2341 aaaagatatc ctttctgaat atcagtagtg ttttgctttc tactatagga gaagttttct
     2401 attgacagtt taagaaaagt gagttattat gctataagtt tacgatatag aaagtgatac
     2461 ctatggcaac tacgacaaaa acgtcgacca aaaaaacaag taaaaaaaaa tcagctactg
     2521 ctaaaaaaaa tctagttatt gtggagtctc ctgcaaaagc aaagactatc gaaaaatact
     2581 taggacgtaa ctataaagtt gtagcctcag taggtcatat ccgtgattta aaaaagtcaa
     2641 gtatgtccat tgattttgag aataattacg aaccacaata tattaatatt cgtggtaaag
     2701 gacctctcat taatgattta aaaaaagaag ctaaaaaggc taaaaaagtt tacctcgcga
     2761 gtgacccgga ccgtgaagga gaagctattt cctggcattt agcgcatatt ttagatttag
     2821 acaaagaaga cagaaaccgt gttgttttca atgaaatcac aaaagatgct gttaaaaatg
     2881 cttttgtgga acctcgtcag attaacatgg atctcgttga tgctcagcaa gcacgacgtg
     2941 ttttagatcg cattgttggt tattctatct cacctatttt atggaaaaaa gtaaaaaaag
     3001 ggctatcagc tggacgtgta caatcagttg ctcttaaact aatcattgat cgtgaaaatg
     3061 agattaaggc tttccaaccg gaggagtatt ggactattga cggttccttt aaaaaaggaa
     3121 cacgtaaatt caatgccact ttctacggtt tagacggaaa gaaattcaaa ttatcgaata
     3181 atgaagacgt caaaacagtt ctaaaacgta ttaaaactga tgaattctta gttgaaaaag
     3241 ttgaaaaaaa agagcgtcgt cgtaatgcac cattaccgta tacaacttct tcattgcaac
     3301 aagatgcagc taataaaatc aattttcgaa ctcgtaaaac catgatgatt gcgcaacagc
     3361 tgtacgaagg acttagctta ggcacagcag gtcatcaagg tctgattacc tatatgcgta
     3421 ctgattctac acgtattagt ccgttagcac aaaatgaagc aacagaattt attactaacc
     3481 gttttggtgc aaattattct aaacatggga ataaagttaa aaatgcttct ggagcacaag
     3541 acgctcacga agctattcgc ccatctagtg ttaatcatac acccgaaagt attgctaagt
     3601 atttagacaa agatcaacta aaactttaca ctcttatctg gaaccgtttt attgcaagcc
     3661 aaatgacagc agcagtcttt gacacaatga aagttaattt aacgcaaaat ggtgttactt
     3721 ttattgctaa tggtagccaa gtcaagtttg atggttacat ggctgtttat aacgatactg
     3781 acaaaaataa aatgttacca gatatggagg aaggagaaag tgttaaaaag gttaatacga
     3841 atcctgaaca acacttcact caacctcctg caaggttttc agaagcaagt ctcattaaaa
     3901 cacttgaaga aaacggtgta ggtcgtcctt caacttatgc cccaacgctt gagactattc
     3961 aaaaacgtta ttatgttaag cttgcagcta aacgcttcga accaactgaa cttggtgaaa
     4021 ttgttaatag ccttattgta gaatttttcc cagatattgt tgatgttacc ttcactgccg
     4081 aaatggaagg gaaactagac gaagttgaga ttggtaaaga gcagtggcaa aagattatcg
     4141 atgaatttta taaaccattt gaaaaagaac ttgcaaaagc agaaactgaa atggagaaaa
     4201 ttcaaataaa ggatgaacct gctggatttg attgcgagct atgtggatca ccaatggtaa
     4261 taaaactagg acgttatgga aagttttatg cgtgtagcaa tttccctgaa tgtcataaca
     4321 caaaagctat cactaaggaa ataggtgtta tttgtcctat ctgtcaaaaa ggacaagtta
     4381 ttgagcgaaa aacaaaacgc aatcgtatct tttatggctg tgaccgctac ccagaatgtg
     4441 agtttacatc ttgggacaaa cctattggac gaacttgccc caaatcaaat gatttcttag
     4501 tggagaaaaa agtacgcggt ggtggtaaac aagttgtttg ctcaaatgaa aaatgcgact
     4561 accaagaaga gaaaattaaa taaagtggaa tctgagtgtt tagctcagat ttttaaaaaa
     4621 attattattt tacataactt aatttatgta tttattttta tgggagaatt aataaaaaag
     4681 tgctataata taggtgtatt catagtgtga ggtgccgtta tgtcctatat tgaaatgaag
     4741 cattcctata agcgttatca aacaggtgaa accgaaattg ttgctaacaa tgatattagt
     4801 ttcagtattg aaagaggtga gttggtggtt attcttggtg catccggcgc tggcaaatct
     4861 actgttctca atatactagg agggatggat agtaatagtg aaggtgaagt tttaattgat
     4921 ggtaagaata ttgctaatta taccatcagg gaattgactc gttaccgtag atatgatgtc
     4981 gggtttgttt ttcaattcta taacttagtc cctaatctaa cagcactaga gaatgtagaa
     5041 ttggcttcag aaattgttcc aaaggcatta gatgcccaac aggcattaga aaacgtaggt
     5101 ttgggacacc gtatcaatca ttttccagca cagctttccg gtggcgaaca gcaacgcgtt
     5161 gcaatagcac gagctattgc caaaaaacct aaactgttac tttgtgatga acctactggt
     5221 gctttagatt accaaactgg aaaacaagta ctagccatcc tgcaaaagat ggcgcaatca
     5281 aaagaaacaa ctgtgatcat tgttactcat aatactgcct tagcgccaat tgcaaatcgc
     5341 gtgatccata tgcatgattc aaaaatcagt gatattgtaa taaacgagaa tccaagtgat
     5401 attcagaaca tagaatatta gaggttatac agatgggaaa gacattttgg aaagatatct
     5461 atcgatcaat tacgacttca aaaggtagat tttcatcaat cctcttactt atgatgttag
     5521 gctcatttgc ttttatagga ctgaaggtct cagcacctaa tatgcaaaga acagcacaaa
     5581 attaccttgc tcatcatcat gttatggata ttaccgtttt caattcttgg ggacttgaca
     5641 aacatgatca aactgtttta gaaagcttaa aaggatcaca agttgaattt tcttactttg
     5701 tagatacaac ccctcaacag aatagtaaat cttatcgatt gtattccaat accaaaacga
     5761 tatcaacctt tgatcttgtt aagggacgtc tccctttaaa taaatcagaa attgctctct
     5821 catttcagga acgaaaaaaa tatgcaatag gtgataaaat taattttaaa caagataaaa
     5881 acaaactgtt ttcaaataca ggtcctctaa ctattgtagg ttttgttaat tctaccgaga
     5941 tttggtctaa gacaaacctt ggtagttctc aaacagggga cggcgactta gatagttatg
     6001 gcgtattaga taagacagca tttcactctc cagtatatac aatggcaagg gtaactttta
     6061 aagatcttag gttgattaac cctttctcaa taagttacaa agaaaaagta gcgaaatacc
     6121 aagaaaaggt atcgcgaaaa ttaaatattc acaataaaat aagatatacc aaaacaaaaa
     6181 aagagagcct acgtaaaatc gatgaggagg aaaaaagcct actaaaagct caaaaacaaa
     6241 tcaatcggtt agataatgat agcctagcga tgccactatc tcaaagacaa gctattcaaa
     6301 tgaaaattaa acaagaccga ctatctcttt tgaaacgaac aaaagagctt ctaaaattga
     6361 gacataacac ccaaataatg gaatcgcctc aaattattgt ctacaaccgt actacctttc
     6421 ctggaggaca aggatataac acttttgact ccagtacaaa tagcacttct aaaatcagta
     6481 atcttttccc cattatttta tatttagtcg cagcattagt aaccttaaca acaatgacta
     6541 ggttcgtcga ggaagaaaga accaacgcag gcatattaaa agcactaggc tatagtgacc
     6601 gccaagttat ctttaaattt attatttatg gttttatagc aggaacatta gggactactt
     6661 tgggtatcat tggagggcat tatcttttac ctcgtattat ttctgatatc atttccaaag
     6721 acctgactat tccaaacaca cagtatcatc ttttcttaaa ttacagttta ctagcctttg
     6781 ttttttcatt attaagcatt gttcttccag tttttgttat cactcgacgt gagttgaaag
     6841 aaaaagcagc atttttatta ttaccaaaac caccagctaa aggatctaaa atagctttag
     6901 agtatatcaa ttggatttgg aaaaaactgt ccttcacaca aaaagtaact gctagaaata
     6961 tttttcgtta caaacaaagg atgattatga ccatatttgg agttgctgga tcagtagcgc
     7021 tcttattttc aggtctagga atacaatcct ccttaaagca aaccgtcaat gaacatttcg
     7081 gtcgtattat gccctatgat atattgctaa catataacac aaatgcttct cccccaaaaa
     7141 tacttgaact actttcaaaa gattcaaaaa tagacaaata ccaaccaata caccttgaaa
     7201 atcttgacga atccattcct ggacaaatta ataaacagtc aatttcccta ttcataacag
     7261 acaaaaaaca attactacct tttatttatt tacaagaagc aacaactaat aagtcattgc
     7321 acttgaataa taaagggatt attatttcta aaaaattagc tcaattttat catgttaata
     7381 ccggtgattt tatccattta tctcattcac aaacacttcc ttctagaaaa ttaaaaataa
     7441 caggagttgt caatgcgaat gttggtcact atatttttat gacaaaacaa tactatcgaa
     7501 ctatttttaa gaaagaagct aaagataacg cttttttggt taagttaacc aaacataaaa
     7561 tcgcaaataa cttagcagaa aaacttttag aaattaatgg agttgagtct cttacgcaaa
     7621 atgctcttca gctggctagt gtagaagccg ttgtgcgttc tttagatggc tctatgacta
     7681 ttttagttgt cgtatcttta ttgttagcca ttgtaattct ctataatctc actaatatta
     7741 atttagcaga acgtaagcgg gagctatcaa ctataaaggt attaggtttt tataatgaag
     7801 aagtaacttt atacatttac cgcgaaacca ttattttatc aaccatcggt gtgattctag
     7861 gcaccattag cggtacttat ttacaccgtc aaatgatgtt gctaattggt tcagatcaaa
     7921 tactttttgg tgaaaaagta tcacc
//
LOCUS       DATRSD010000079         7853 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRSD010000079 DATRSD010000000
VERSION     DATRSD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.080"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(257..997)
                     /locus_tag="VBD85_001645"
     CDS             complement(257..997)
                     /locus_tag="VBD85_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7134187.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(997..3180)
                     /locus_tag="VBD85_001646"
     CDS             complement(997..3180)
                     /locus_tag="VBD85_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7134188.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELIKKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            3363..4283
                     /locus_tag="VBD85_001647"
     CDS             3363..4283
                     /locus_tag="VBD85_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7134189.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            4344..6335
                     /gene="uvrB"
                     /locus_tag="VBD85_001648"
     CDS             4344..6335
                     /gene="uvrB"
                     /locus_tag="VBD85_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO7134190.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEVFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAEIKALQQQMQEAAELLDFELAAQIRDVILELKAID"
     gene            complement(6432..>7853)
                     /locus_tag="VBD85_001649"
     CDS             complement(6432..>7853)
                     /locus_tag="VBD85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134191.1"
                     /translation="LSLDTACNRLHLSPVELKNYCHKLNVLFGHKLSIHIQKKRILCK
                     LNKLTQEDALKIVYNESNTLQLLKFLIDDSSDKKSLVAFGNDLFLSRSSVYRLRENLI
                     PLIKDLGLSLSKNTIVGDEYRIRYLIAYLTTKFGIKIYDLSALDIKIIRQFLFESGTN
                     IRPTTHLSDIFVFFDTLFILTWKRHHHKVKIPNSKLFSSLKKIFIYEELNTCVKSIVQ
                     PYFNVIFNSEDMDYIFLIYLTSANSFAAQKWTETHVDEVLAIFKSLPKFQMLLQPLKE
                     ALPYSDTYYDELVKVTIYFSRNFIVGLNQLIPETLSFPSTFYPGNNPKLISIIEPIVT
                     HWLTEIGESKFKKTHFFLLCAHLERVIKNSMPPINIVLLTTDFINNEILKDYILQSLS
                     TDKIRFHSYYLLTDNISQISTLKPDLIITNRKLFTYVTKELATNSLVAHIDYNDTPSQ
                     ISNIQTIISNIKEEKYRKIFDKI"
BASE COUNT         2495 a         1242 c         1481 g         2635 t
ORIGIN      
        1 gttactcacc ttttcccatt taatatctaa aaagtcatct ataaagttga catgataagt
       61 catatgttct ttaataaagt gcaatattct gttaatatag tctttcatac tgctatatta
      121 tcatactgaa agcccttgtg caacattatt caaacattct agctgatgtt ttgctaattt
      181 tagaagaaaa cttatcctat tataatttgt ttgtgattag gatataaaaa aagatgacac
      241 gaagtcatct tttttattaa acgtttaaaa ctttattaag gaagtcttgg agacgtgggt
      301 gttgaggtgt atcaaagatt tgctcaggtg tcccatcttc gaggaaacga ccaccatctg
      361 taaagataac acgattggca acttgacgag caaatcccat ttcgtgtgtc acaattaaca
      421 tagtcatacc ttgttcagct aaatctttca taacattgag aacatctcca accatttctg
      481 gatcaagtgc agaagttggt tcgtcaaaaa gtaagatatc tggattcatc gctaagcttc
      541 ttgcgatagc tacacgttgt ttttgtcctc cagataaact atctggtttg gcattagctt
      601 tatcggctaa accaactttt tcaagtaatt ccatcccatg cttttcagct gcttctttag
      661 attctttacc aagttctata ggagcaaaag tgatgttttc aagaactgac atatgtggga
      721 aaagattgaa gtgttgaaat accattccga tattttcacg tgctttgtct atgtctgttt
      781 ttgggtttga taactcaaag ccgtccacaa caacttttcc acttgtgatt gattcaagta
      841 agttcaatgt gcgaaggaaa gttgattttc ctgaaccaga aggtccaata atacaaacaa
      901 catcaccttc ataaaattta gcatcaatac cttttaaaac ttcattttgc ccatacgact
      961 tgtgtaaatc ttggacatca atttttaatt ctgccattat ttaagcctct tttctaaacg
     1021 ttttgctaga cgggtgagaa gggtaatgat tacaagatac ataattgcaa ggatagcata
     1081 catacggaat gattgatagt tacgtgcaat tatgatttta ccagtttgga agagttccac
     1141 aagtccgatt gctgatacaa ttgttgtatc ctttaatgag atgacaaatt ggttgataaa
     1201 gtttggtaac ataaggcgta ctgcttgagg caagataacc ttttgcatag ttttgccgta
     1261 agaaatacct aaactacgac ttgcttccat ttgaccagaa ggaacagctt caataccacc
     1321 acgtacaatt tcagcaatgt acgcaccacc atttaaagaa agagcgatag tagcagcaac
     1381 gaagtcatta attggacttt ggtgacctgt gatgctttcg attaaattag gaatacccca
     1441 gaaaataaaa gcggccacaa tcatgagtgg aataccacgg acaatatcaa caaaaatcat
     1501 tgaaattgtg cggagagtat tacttggtga tacgctcatc ataccaaaga taataccaat
     1561 aaccatagca atcgcaaatg agataagagt taaacttaaa gtagttccaa taccagatag
     1621 caattgtttg tagttattag aaattaaccc taaaatagtt gattcatcta caggtttagc
     1681 agctttatcg tttgaagagg tgctggctgt ggaaaggtat tttttaacaa gtttatcgta
     1741 ctcacccgat tttttgagtg aagcaagacc gttgttaaac attttaatta attctggatt
     1801 tgcccctttt ttcactgcaa atccgatatc gcctgatttt tcacctttga taggtgtttc
     1861 aaatttacga ccttgattaa tagcgtaagc aagaactgct tcgtcatcca ttagagcatc
     1921 aattgaacct gagttcatac tatcatacat tgtagatgct tcatcaaatg ctttaacatg
     1981 atagttgtac ttatctgcgt ggtctgataa ccaagtatat gaggcagtac catttttagc
     2041 accaactgtt tttcctttta aatcttggta tgatttgaca ttgcttcctt ttttaaccgc
     2101 taagataacg ctagatgtgt aataaggatc agaaaaatca aagatttttt ggcgtgcttc
     2161 tgtgattgta gctcctgcaa taacaccgtc agcttgtcca gattggacag catttaaagc
     2221 ggcatcaaaa cctggatttg agatatcaag tttaaaacct tgctgtttag caattttctt
     2281 gattaattcc atatcaaaac cagtatattt ccctttaccg ttttgatatt cgaatggtgc
     2341 gaatgaagaa tcagaaacaa ttttataact tggctttaca ggagttgctt tttcattggc
     2401 attacctgtt gctttgccgc ttgtttttgt agcgtctttc ccaagccatt tatccatgat
     2461 gtcattataa gtgccatctg atttcatttg tgcaaaagct gtgttaaatt ctttaattag
     2521 gttatcgtgt ccactacctt ttttgacagc aaatgcaaag ctaccaactg cctcgccttc
     2581 catgttaatg gcgtaagctt ttccttgatt tatcgcaaat tgcacaactg gttgatcgtc
     2641 catagcggcg taaatagaac cagaatcaag gctgttattc attaggtcac ttgtatcaaa
     2701 tgttttaact ttatagccgt atttagattt attttcctct aagaagcttt gagcagctgt
     2761 tccatttttt acaccgacta cttttccttt tagttgtttg tagtttgtga ctttattatt
     2821 atttttagta taaagaataa cggaagtatc gtaataagta tctgagaaat taaagacttt
     2881 tttacgtgct tcagtaacag tagttccggc cattagcgcg tctgcctgtc cagattgaac
     2941 agcgttaact gcggcatcaa aacctgggta cgtcatgtta acattccagc cagcacgctt
     3001 agcgacttcg ttaacgatgt caacatcgat tcctttataa gtttgatcag aatctttaaa
     3061 ttcgaatgga gcgtatgctg tatcagaaac aatattcaca gtctctgcac tgacctttcc
     3121 accaaacaca aagaatattg agagtaaagc aagcgtaaaa gcttttaact tgtgcttcat
     3181 gtaaatctcc tttttataaa tatgtgtttt attatatcaa aataaataag atagagcaac
     3241 aaaatgtcat ttctatgtta gaaaacgtaa catgtatgtt atgttaattt tatatatatc
     3301 tttagttagt attatgatat aatcgttcta gtgttatcac acaaaaagga tttaggatta
     3361 ttatgaataa aaggatttta aatagtcagt tcgctgtcta taaaaactgg ccgtcatggc
     3421 tattattgtt atttgcttgt tttatggtat acctattttt tttcgttggt ctaactctag
     3481 gtggtgtggg aattgttcta gtgtcttctg tactctataa gttttcttcc tatacttttt
     3541 ttgagtttat taatttattt aataatatct atggagaatt aggaaccttt agtttttcag
     3601 cattcctttt gcttatctgg gtaaaattcg tagaaaaaag acctttttca gaattggggt
     3661 tttctacaaa gtttaaaaga actctgtggt cacttataaa aggctggagt ataggtttta
     3721 ttctattttc aatttctgtc atcactgctt atatattggg aggacttgac tttcatagct
     3781 atgatgtttc taaggcaacg attttctatg ttgtaactct ccttcctttt tggcttattc
     3841 aaagcggtac ggaagaattg ctaacacgag ggtggcttct tcctctaatt aatcatcgtt
     3901 ttcatttagc tgttgccata ggggtctcat ccactttatt tggcattttg cacttggtaa
     3961 atgcccatgt cacttttctt tctattgtaa gtattatatg ttcgggagtt ttaatgtcct
     4021 tgtatatgat taaaagtggt aatatttgga gtgttgcagc cttacatgga gcttggaatt
     4081 ttagccaagg taatctctat ggtattgccg taagtggtca gaaagcagga gcatctcttt
     4141 tgcattttac cgttaaagaa aacgcaccgg actggatatc aggtggtgca ttcggaatag
     4201 aaggaagttt gatttctatt tttgttctct tagcagcaat tatatacctt ctatggctaa
     4261 ttaagactga agaaacagat tgagtgttgc agctcagtct ttttcttttt tctatgttaa
     4321 aataaagtaa tcgaggtaaa gaaatgatag atagaaaaga tactaaccga tttaaattag
     4381 tttccaaata tagcccttcg ggagatcaac ctcaagctat tgaaacgttg gttgacaata
     4441 ttgaaggagg agaaaaagct cagattttaa aaggtgctac tggtactggt aaaacctaca
     4501 caatgagcca agttatcgcc caagttaaca agccaacctt ggtgattgca cataataaaa
     4561 ccttagcagg tcaactttat ggagagttta aagaattttt tcctgacaat gccgtagagt
     4621 actttgtttc atattatgat tattatcaac cagaagctta tgtgccttct tcggatactt
     4681 atattgaaaa agatagttca gtcaatgacg aaattgacaa attacgtcac tcagcgactt
     4741 cttcgttatt agaaagaaat gacgttattg ttgtagcatc tgtttcttgt atttacggtt
     4801 taggttctcc taaagagtat gcagacagtg ttgttagcct taggccaggt caagaaattt
     4861 cacgtgatca gttattgaac aatctcgttg atattcaatt tgagcgtaac gatattgatt
     4921 ttcaacgtgg aaaatttcgc gttcgtggcg atgttgtaga agtttttcct gcgagtcgtg
     4981 atgagcatgc ctttcgaatt gaattttttg gtgatgaaat tgatcgtata agagaaatag
     5041 aatcgttaac agggcgtgtg ctaggagaag tggaacattt agctattttc ccagcaacac
     5101 actttatgac aaatgatgaa catatggaag aagctatttc aaaaatacaa gctgaaatgg
     5161 aaaatcaagt agaacttttt gaaaaagaag ggaaattgat tgaagcgcaa cgtattcgac
     5221 aaaggacaga atatgatatt gaaatgcttc gtgaaatggg atatacaaat ggtgttgaga
     5281 actactcacg tcacatggat ggtagaagtg aaggtgagcc accttttact ttgttagatt
     5341 tcttcccaga agattttttg attatgattg atgagagtca catgacaatg gggcaaatca
     5401 aagggatgta taatggagac cgttcgcgta aagaaatgtt ggttaattac ggtttccgtt
     5461 taccatctgc cttagataac cgtccacttc gtcgtgaaga attcgaaagc catgtccatc
     5521 aaattgtcta tgtatctgca acgccaggag actatgaaat ggagcaaaca gatacagttg
     5581 ttgagcaaat tatccggcca acaggacttc ttgatccaga agttgaagtt agaccaagta
     5641 tggggcagat ggatgacttg cttggtgaaa tcaatttgag gactgaaaaa ggtgagcgta
     5701 cctttattac aacattgaca aagcgtatgg cagaagactt aacagattat ctcaaagaaa
     5761 tgggagttaa agttaagtat atgcacagcg atattaaaac cttagaacgt acagagatta
     5821 ttcgtgattt acgacttggt gtgtttgatg ttttaattgg tattaatctc ttacgtgaag
     5881 gaatagatgt tcctgaggtt tcccttgttg caatcttaga tgctgataag gaaggtttcc
     5941 ttcgtaatga gcgtggtctt atccaaacaa tcggacgtgc agcacgtaac agtaatggtc
     6001 atgttattat gtatgctgat aaaataacag attctatgca aagagctatg gatgaaaccg
     6061 ctcgccgtcg tcgtttacaa atggattata atgaaaagca tggaattgtg ccacaaacaa
     6121 ttaaaaaaga aatccgtgat ttaattgcta ttactaaaag taatgacagc gataagcctg
     6181 aaaaagttgt cgattacagt agtttatcta aaaaagaaag gcaagctgaa attaaagctc
     6241 ttcaacagca aatgcaagaa gcagctgaat tattggactt tgagctagct gcacaaatcc
     6301 gagatgttat cttagaacta aaagcaatcg attaatacga tgaacagaat taataaaacg
     6361 actagagtag ttaatgaagt gctctagtcg ttttattaat aattgaaatc aaaagaatac
     6421 agtaggtgat gttatatttt atcaaagatt tttcgatatt tttcttcttt aatattagaa
     6481 ataatagttt ggatattact gatttgagag ggtgtatcgt tgtaatcaat atgagcgact
     6541 aaactatttg tagctagttc ctttgtaaca taggtaaaca gttttcgatt agtgataatt
     6601 aaatcgggtt ttaaggtcga tatttgacta atattatctg tcaataagta ataggaatga
     6661 aatctaattt tatctgttga aagggattgc aaaatatagt cttttaaaat ttcattatta
     6721 ataaaatcag ttgtcagcaa cacaatattt ataggaggca tgctattctt tattactctt
     6781 tcaaggtgag cacagagcaa gaagaagtgc gtttttttga actttgattc tccaatttca
     6841 gttagccaat gagtgactat tggttcgata atggatatta gtttaggatt atttccagga
     6901 tagaatgttg aaggaaaact aagtgtttca ggaattaatt gattcagacc aactatgaag
     6961 tttcgagaaa agtaaatagt gactttcact agctcatcgt agtaggtgtc agagtatgga
     7021 agagcttctt tcaaaggttg tagtagcatt tggaatttag gaagactttt aaaaatagct
     7081 aacacttcat cgacatgtgt ttcagtccac ttttgagcag caaaagagtt agcgcttgta
     7141 agatagataa gaaaaatata atccatgtct tctgaattaa aaataacatt gaagtaaggt
     7201 tgaacgatac tttttacaca tgtattcagc tcctcataaa taaagatttt tttaagagaa
     7261 gagaaaagtt tggagtttgg aattttaact ttatgatggt gacgtttcca ggttaaaatg
     7321 aaaagcgtat caaaaaaaac aaagatatca gaaaggtgcg tagtaggtct tatattagta
     7381 ccactctcaa ataaaaattg tcgaataatc ttaatatcta gagcagacag atcgtaaatt
     7441 ttaataccaa atttagtagt gaggtatgcg atgagatatc taatgcgata ttcgtcacct
     7501 acaatggtat ttttagataa agataatcct aaatctttaa ttaaaggaat taaattttct
     7561 cgcagacgat agacagaaga acgagataag aagagatcat ttccaaaagc tactagagat
     7621 tttttatcag acgagtcatc aattaaaaat ttaagaagtt gtaaagtgtt tgactcatta
     7681 taaactattt ttaaggcatc ttcctgagta agtttattta gcttacataa aatccgtttt
     7741 ttttggatat ggatagaaag tttatgacca aataaaacat ttaatttatg acaatagttt
     7801 ttaagctcaa ctggtgataa gtggagtcgg ttacaggcgg tatctaagga tag
//
LOCUS       DATRSD010000080         7591 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRSD010000080 DATRSD010000000
VERSION     DATRSD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.081"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(74..2869)
                     /gene="ppc"
                     /locus_tag="VBD85_001650"
     CDS             complement(74..2869)
                     /gene="ppc"
                     /locus_tag="VBD85_001650"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO7134192.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSLTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSINLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSSPKEKFVSRQESSDIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQYGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHAPKSELLQKELAIFQTARELKDQLGED
                     IINQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYELVKKWIATNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNPNEIDNFRETMDGIVSESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKHFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            3078..4877
                     /gene="pepF"
                     /locus_tag="VBD85_001651"
     CDS             3078..4877
                     /gene="pepF"
                     /locus_tag="VBD85_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7134193.1"
                     /translation="MKLKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSRTPLETALLIS
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIEQG"
     gene            complement(4936..5325)
                     /locus_tag="VBD85_001652"
     CDS             complement(4936..5325)
                     /locus_tag="VBD85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO7134194.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5309..5833)
                     /locus_tag="VBD85_001653"
     CDS             complement(5309..5833)
                     /locus_tag="VBD85_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7134195.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTITMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            5990..6838
                     /locus_tag="VBD85_001654"
     CDS             5990..6838
                     /locus_tag="VBD85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO7134196.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRVFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKE"
     gene            complement(6871..7488)
                     /locus_tag="VBD85_001655"
     CDS             complement(6871..7488)
                     /locus_tag="VBD85_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7134197.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIRGDITEADKIHLEDRTFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTTLRKKPTQKILSIEELNNK"
BASE COUNT         2484 a         1386 c         1213 g         2508 t
ORIGIN      
        1 taccattatg cattatatca tctttcgaag taaagtaaaa gggagaatag caatgatcct
       61 ccctctatca atattaacct gaatttcgta aacctgttgc aataccatta atagtaatat
      121 gaatcaactt ctcatagtct tcatctagtt ggttactacg aagacgttta atcaattcaa
      181 tttgaacata attcaaaaca ttgaaatatg gcaaacgata atctaaactt gcatgtaaca
      241 ttgggttttc ttctaataaa ttatcatgct gttcaatagc taaaatcata tctttagtta
      301 actgccattc attaaggatg atattaaaaa catctcgaac ttctttactt ccagctaact
      361 gagcatactg aagcgcaata ttcatatttg attttgataa aaccatatct acattagata
      421 aaagagaatt aaagaatggc catttttgat acatggtttg taattttgct aagtttgcct
      481 catcttgttc aataaagtgc ttgaaggctg atccaacacc ataccatcct ggaaacataa
      541 tacgattttg tgaccatgaa aatacccatg gaattgcacg taaacctgaa atttctgtaa
      601 ttgttttacg tgctgctggc cgagaaccaa tatttaaact tgatacctct ttaattggac
      661 ttgcttcaaa gaaatagtca tagaaataag gattatcaaa aactaaattt ctataaacgg
      721 cattactttc agacacaata ccatccattg tctcacggaa attatcaatt tcattagggt
      781 tagtaatcat acgtgttacc atgcggtcta tggaagctga aataagcatt tcaaggttat
      841 aataggctgc atcttgatta ccatatttat tctcaatgat ttcaccttgc tctgttaggc
      901 gaattcgatc tttgattgaa ccaaaaggtt gtgatgtgat agcttcataa gatggaccac
      961 caccacgacc aacagttcca ccacgaccgt ggaaaaatgt gattttaata ccgttttctt
     1021 cacctatttt tgttagctca ttttgagctt tatatagagt ccaaccagat gataaatagc
     1081 caccatcttt attactatct gaataaccta gcattatttc ttgataattg ttgttagtag
     1141 caatccattt tttaactaat tcataatgta gatactgggt cataatatca cgtgaattat
     1201 ccaaatcttc gatggtttca aataacggta caatctgaat acgagcttga ttggcatcaa
     1261 ttagtccaac ttcttttaac atgatagcta actcaaacat atccgaaacg ctctctgtgt
     1321 gagaaatgat atgctggttt ataatgtctt cacccaattg atcttttaac tcacgtgctg
     1381 tttggaaaat agccaattct ttttgaagta actcggattt aggagcatgc gtagaagata
     1441 atgttctagg atcttctgtt aattctttta agagaagttg acatttttct tcttctgaaa
     1501 gagaactgta atcatctaca atattggcag acttcaataa ttctgctaca caagcttcat
     1561 taatactaga atcttgacgc atatcaatag tagctaaata aaatccaaag atatctactg
     1621 cttgaataag acaagctaat tcgccatcaa caagagaatc ttgtccatat tgaactaaag
     1681 attgcttcac aagtaacaaa tcttgtttaa actcctcagc agtagcgtat gctggtagtt
     1741 cttctgtctg tatgtcagaa gctacttgag cgatatgtga ttttatatac cttcctacaa
     1801 tatcagaaga ttcttggcga ctaacaaatt tctcttttgg ggaagaacca acttttaaat
     1861 ttagtagggt ttgtaccagt ttatcttgga taaaattgaa agctttacgg taaggttcat
     1921 tttccctata taccgaatta tcttgtgact ggtttgccaa tgtaacaagt tcgggagaga
     1981 cttcagttag atttattgaa agtgacatat tacgataaag ttcatttagc tgctcaatat
     2041 aatggttaat aatcacttca ctttgtacca tagctgataa tcttagtgtc tcagcagtta
     2101 caaagggatt gccatctcta tcaccaccaa tccacatccc catagtcaga ggcttaggat
     2161 tctccaagtg aatacctttt ttagctgcca gtgccttata ttctgctgtt aatttggtaa
     2221 ctgctttaat aagagaacgg ttgtaatatt ccattacatt ggttatctca tttttgactt
     2281 tcaatttttt ctcgcgaata gtgtctgttt gcatgataat gccgatgtaa cgacgcaagt
     2341 cagcatacca tttttcttga ttcacgattc ctgccttgac atctctgtat ttacgtaata
     2401 aatcatgaat cttagacgtt aattcgagca ccgttttacg ctgcacctga gtaggatgag
     2461 ctgttagcac aggcacaaca ttgacgtgtt caagaatatc tttagcattt tcatggccag
     2521 ctacaacatc aatagtcgtt gataactttc ctaggtaatc ttggttaaga ttatttttat
     2581 aattaatttc atatgctaaa tcaacgtctt ctgaaatatt aattaagagc ggcaaaatag
     2641 agaagtaacg agaaacaata accatctcct cgttgcttaa agccgaaata gtttccttca
     2701 gaccagtata atcgtcagtt aatgataatg aaactatttt ttcaatcttg tcaaatgatt
     2761 cactaccaat caatttttgc gttgcttcat ccaaaagctg cttcagtaaa ccgacctctt
     2821 cggtaatgat ttctttattg ctactacttt ctaatttcgg gtgggacaaa ccatcacttc
     2881 ctctcttaaa tctcactact atgataccat tttaaagtaa aaagtaaacc attctaggat
     2941 ttttagatag aattttcaga tttttactta tttatgacag taatcagttt ttcttgttcg
     3001 acttttttga tatactgagg ataattgaga ttaactgatt aattgttaat taacaactat
     3061 tattaaagga gtatcgcatg aaacttaaaa aacgctctga attccctgaa aatgaacttt
     3121 gggatctgac tgctctctac aaagatcgtc aagatttttt actggctatt gaaaaagctc
     3181 ttgaggatat caaggttttc aaaaaaaatt acgagggaaa acttaattgt gttgaggatt
     3241 ttactagtgc ccttatggaa atcgagcata tttatatcca gatgagtcac attgatacgt
     3301 atgcttttat gcctcaaact actgacttta gcaacgaaga gtttgcacaa atttctcaag
     3361 caggatcaga cttcgcaact aaagctaatg tactactttc tttctttaat actgccctag
     3421 caaatgcaga tataaagatt ttagattctt tagaaaataa ccctcatttc aaagcaacta
     3481 ttcgccaagc taaaattcaa aagcaacact tattaagtcc agaagtcgaa aaagctttga
     3541 caaacttaaa tgaagtccta aatacaccat atgatattta tactaaaatg cgtgctggcg
     3601 attttgatat ggaagatttt gaagttgacg gaaaaaccta caaaaatagt tttgtaactt
     3661 atgaaaatta ctttcaaaat cacgaaaatg cagagatacg tgaaaaatcc ttccgttcat
     3721 tctcgaaagg tctacgtaag caccaaaatg ctgcagctgc ggcttatctt gcaaaagtca
     3781 aatctgaaaa gttaattgct gatatgcgtg gttacgatag tgtttttgat tatcttcttt
     3841 ccgaacaaga agttgaccgt tcaatgtttg acagacaaat tgatcttatc atggatgaat
     3901 ttggtccagt agctcaacga ttcctcaaac atatcgccga tgttaatggt attgaaaaga
     3961 tgacttttgc agattggaaa ttagacattg ataacgaact taatcccgaa gtaagtatca
     4021 atgatgctta tgacttagta atgaaatcag ttgctccttt aggaaaagaa tattctcaag
     4081 aggttgagcg ctatcaaaaa gagcgttggg ttgattttgc agctaacgct aataaagatt
     4141 ctggaggata tgctgcggat ccttacaaag ttcatcccta tgtactcatg agctggacgg
     4201 ggcgtatgag cgatgtctac acacttattc atgagattgg acattctggt caatttatct
     4261 tctcagataa tcaccaaagt tttttcaata ctcacatgtc cacttactat gttgaagctc
     4321 catctacttt taacgaactt ttacttagtg actacttaga aaaccaattt gatacagcac
     4381 gtcaaaaacg ctttgctctt gcacatcgct taactgatac ttacttccac aatttcatta
     4441 ctcacttact tgaagctgct ttccaacgta aagtttacac tctaattgag gagggaggaa
     4501 cttttggagc agaacaatta aatgctatta tgaaggaagt attaacacaa ttctggggag
     4561 atgctattga aatagatgac gatgccgctt tgacatggat gagacaagct cattactaca
     4621 tggggctcta tagctatact tactctgctg gattagtaat ctcgacagcc ggctatctca
     4681 atctcaaaaa taatccaaac ggagcaaaag agtggttagc attcttgaaa tctggtggga
     4741 gccgtacccc tttagaaaca gctctattaa tcagtgctga tatttctact gataagcctc
     4801 tgcgcgatac tattaatttc ttatctaaca ctgtagacca aattattaat tacagtaatc
     4861 ttattgagca aggctaaact acagcctttc aaaaacataa aaacaagttg gagatatctc
     4921 caacttgttt ttatcttatt ttttgtaaac ttcctgataa aattcaattt tgtcattctt
     4981 tttaacttta atttggtttg ctgccttagg agctaattta ttattgacct tgaacatcca
     5041 ataaagccct ttggtttcat cttggctgat gccatcaatt gaagtaatga aaccatcttt
     5101 ttctttaact ttatagttag ctttaagcac atccaaaaca gtatcacctt tatggtaaac
     5161 aaccttttca cttaacacgt tcgtatcttc tttaaccacc aattcaacct taccaacttc
     5221 taacgttgag ctagttgttg tcttattcga tgtagatggt tgcccgcaag ctactaaagt
     5281 tgtagcgaat actgttagca agaataattt aatgtatttt ttcattccta aatctcctta
     5341 aaatcggtaa caaaagagga taaaataaac aagttgataa agcgtgtgcc atattaaaac
     5401 taagtcctgc taatacataa gtccaccaag gcatatgata taaccaagca aaacaggtat
     5461 caataatgac gccataaaca acccctaacc ctcctgcaaa gaaagtttga agtactacct
     5521 ccgttatttt accaaaacat acagcttttg tcagcggata tatcagaaat ttccacaagc
     5581 ataaaattaa agtgaaggaa atgatttgca aaaagaccca tggcccaaag cccattaaaa
     5641 aagaagaaac tagcattgtt atagttacag tagctacacc ttcttttaaa tcgaataaga
     5701 caactgtaat caaaaaaatg gcagtaattg gctgaatgtt aggcagaggc gcaaaagcat
     5761 atctcaacac aacacacaaa gcagataata tagcgactct agtcaattta gttaaagttg
     5821 aattctttat catgtcttta attatacctc aaaactatga ctaccgctta aaagtctttt
     5881 ttattacaat ttcatttcta atgttacata aacgacctct ttttttaaaa gaacataaat
     5941 aagcctgtaa actttgattt tttaaacctt ttaagttaca atatcaataa tgagaagaag
     6001 aattaaacct attgttgttg cggttttttt cagtttattt ggacttttat taattattgg
     6061 tcatttacat tcgacaaata ctttgaaaaa agaattagtt gaagctaaaa agacaattcc
     6121 atccgtaaaa gcttcaaaag taccgcaaaa atcaacatca tcgaaagata aagagtttgt
     6181 tcttaaaccg attatcgatg tctctggttg gcaacttcct aaggagattg attacgatac
     6241 gctttcaaaa aatatttcag gtgttgttat tcgtgtcttt ggtggatcaa agatatctaa
     6301 gactaataac gctgcttata caactggaat cgataaatcg tttaagaccc atatcaaaga
     6361 atttcaaaag cgaaatatcc cagtagctgt ctacagttat gcacttggtt caagtgttaa
     6421 agaaatgaaa gaagaggctc agatatttta taagaatgca gctccttaca aaccaacttt
     6481 ttattggatt gacgtagaag aggagacaat gtctaacatg aataaaggtg tccaagcatt
     6541 ccgaaaagaa ttaaaaagac ttggtgctaa aaatgttggt atctacattg gtacttactt
     6601 tatgactgag caaggcatct ctgtaaaagg atttgacgct gtttggattc caacttatgg
     6661 tagcgattct ggatactatg aagcggctcc gcaaactgaa cttaaatacg atttacacca
     6721 atacacctct caaggttatc taccaggatt caatcaaccg cttgatttaa atcaaattgc
     6781 agttaataaa gacaagaaga aaacttatga gaaacttttt ggaaaagtaa aagagtaaaa
     6841 gctactgtta acagtagctt ttttgataaa ctatttatta tttaattctt caatagaaag
     6901 gattttttgg gttggttttt tcctaagcgt agtaacgatt gcttctgcca ctatcacaac
     6961 cttagttgga aagacctttt gtacaacaat acctaagaaa ggcaaatgac taaataactt
     7021 tatacacttg gcttggaaaa tcgagagagg tcgctcttca ccatacatca aacctggtct
     7081 tacaaaaaga taatccagac cgcttgcttt gattatctgc tctgccttcc ttttactttt
     7141 aatgtaagct gaatagccgc tgttggctga aatataaact aactttggta tttgattttt
     7201 gtgacagagt gctactgctt tttgggttgc tttaacgtta agctcatcta gttgattggg
     7261 cttaatcgct ccaatacagt caattaatat atcaaaagtt ctgtcttcta aatgaatctt
     7321 atcagcttct gtaatatctc ccctaatgta ggttaatcta ggatccttaa atatatcacc
     7381 tttaccttca tgtcttgata agtaagccac tttatgccct tttgtaagcg ctgcttttat
     7441 tatctgcttt cctaaaaaac cactaccacc tgcaatcagt atttccatag ttccctctcc
     7501 ttcattttgt aaatttagta tagaaatttt tatatgcctt gtcaaagtaa tcgtttaata
     7561 cctgaccacg gtatgatttc aagtcattac a
//
LOCUS       DATRSD010000081         7589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRSD010000081 DATRSD010000000
VERSION     DATRSD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.082"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..565
                     /locus_tag="VBD85_001656"
     CDS             <1..565
                     /locus_tag="VBD85_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7134198.1"
                     /translation="AKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFVMGQSAVG
                     HTDILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPP
                     YGATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTTI
                     DALPTIMDYLTTQGYYFVTVGELYSTP"
     gene            complement(598..1680)
                     /locus_tag="VBD85_001657"
     CDS             complement(598..1680)
                     /locus_tag="VBD85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7134199.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSNQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVTELVLHNQKLHLK"
     gene            1825..2454
                     /gene="udk"
                     /locus_tag="VBD85_001658"
     CDS             1825..2454
                     /gene="udk"
                     /locus_tag="VBD85_001658"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO7134200.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKITMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            2541..3038
                     /locus_tag="VBD85_001659"
     CDS             2541..3038
                     /locus_tag="VBD85_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO7134201.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            3038..4702
                     /gene="dnaX"
                     /locus_tag="VBD85_001660"
     CDS             3038..4702
                     /gene="dnaX"
                     /locus_tag="VBD85_001660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO7134202.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQINGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGEDSHSSDTFIANLNVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            4791..4985
                     /locus_tag="VBD85_001661"
     CDS             4791..4985
                     /locus_tag="VBD85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO7134203.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(4966..5901)
                     /locus_tag="VBD85_001662"
     CDS             complement(4966..5901)
                     /locus_tag="VBD85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO7134204.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDRTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            6086..7282
                     /locus_tag="VBD85_001663"
     CDS             6086..7282
                     /locus_tag="VBD85_001663"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO7134205.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDENDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            7269..7544
                     /locus_tag="VBD85_001664"
     CDS             7269..7544
                     /locus_tag="VBD85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134206.1"
                     /translation="MLANNKKRKRKFTLFLLMIIYRTFIKSYNKLVKYRKYCIFLENM
                     VYIVNKMMAKLIEFSYRKIFQKNNFTLKSFDYQINIAFIKILYKYKM"
BASE COUNT         2480 a         1195 c         1422 g         2492 t
ORIGIN      
        1 agctaaagca ccacgtgttg cacttacctt tgatgatgga ccaaatgaaa aaacgactcc
       61 taaagcctta gaaatcctta aaagatataa tgctaaagcg actttctttg taatgggaca
      121 aagtgctgta gggcacacag atattttaca aaggatgcat gctgaaggtc atgagattgg
      181 taatcataca tgggatcatc ctaatttaac caaactacca gcggagaaaa tcaaggaaga
      241 gattcataag actaatgatc tgattatgaa agcaacagga caaaaaccgg tgtacttacg
      301 tcccccttac ggagcaacaa atgctactgt aaaaacagtg acaggattga aagaaatgtt
      361 gtggtcagtt gatacagaag actggaaaaa tcataataca caagcaatga tgaccaatat
      421 caaaaaacaa ttacgtcctg gaggagtgat tttgatgcat gatattcacc agacaaccat
      481 agatgctctg cctacaatta tggattatct gacaacccag ggctattatt ttgttactgt
      541 aggtgaatta tattcaacgc cttaataaaa aaagaaacaa gttacttgtt tcttttttta
      601 ttttaaatgt aatttttgat tgtgaagtac aagttctgta acggtggcgt attttttaag
      661 ttgtttgagc tcttctttat gtgttacaaa tgtaataaca catccctctt tccccatacg
      721 accagttcgt ccagaacgat gtgtatatgt ttctttgtcg cgtgcgatat caaaattaat
      781 cacatattct aggttgtcga tatcaatgcc acgagctacc aaatcagttc ctaggagtaa
      841 tgaaatatca tggtttttaa atttctctaa gataacttta cggaatttaa tattaatatc
      901 gcttgctaaa gaaactgccg aggccctatt aaattgtaaa cgttcttcac atgcacctaa
      961 gtctgataag ctgttaaaaa agacaagacc tctaaaatca ggaatatttg aaaatttacg
     1021 aagtaggtca gtacgctctc ttttatcaac tgtaatataa taatgtttta tagtatcaag
     1081 tttttgatta gatagatcga ttgttatagt gttatcggca agtttactat tgtccagttt
     1141 gttggtggca ctaatgtaaa tcatttgctg atcacgagga acgcgattaa taatattgtc
     1201 cacaaaatga tattgtgagt cgccgagtaa ttcatcgaat tcatcaagga cgatagtatt
     1261 aatgttcatc atttttattt tttttagttt cacaagttca aatacgcgtc cagcagttcc
     1321 tataagaatt tctggtcctt tttttagtcg ctcaatttgg cgtttttgac tagaacctga
     1381 aaggaatagt tgtgcggtca gtcctaatgg ttcagcccat tcttttgtaa cgtcgaagat
     1441 ttgtccagct aattctgaat ttggagctaa aattaagagt tgttggctct ttttaggttg
     1501 caacttaaga agcgttggga aaaggtatgc tagagtttta ccagtacctg tagggctaat
     1561 tccaaggata ttatcaccat cagtaatagg ttgaaaaagt ttattttgaa tatctgttag
     1621 atcatcaaat tgacgctggg ttaatttatc ctgccattgg tctggaaatt ttgttatcat
     1681 aagaatcctc ttgttatttt atatattatc attgttatta taacatgctt tttattttta
     1741 gagcgttttc aataaaattt agactttaaa aaaaataagt ttatagtaca atagatagag
     1801 gataaattta atttggagtg acaaatgcgt aaaaaaccta ttattattgg tgttacaggt
     1861 ggatctggtg gtggtaaaac cagtgtttct cgggcgattt tatcaaattt cccagatcaa
     1921 aaaattacca tgattgaaca tgattcttac tataaagatc aaagtcattt aacttttgaa
     1981 gaacgtgtaa aaaccaatta tgatcatccc cttgcttttg ataccaattt gatgattgag
     2041 caattgaacg aattgattga gggacgtccg gtcgatattc cggtttatga ttacacaaaa
     2101 catacccgta gtgatagaac tatccgccaa gaaccccagg atgtcattat tgttgaggga
     2161 attttggtat tagaagatca gcgtttgcgt gatttaatgg atatcaaatt atttgttgat
     2221 acagatgatg atattcgtat tattcgtcgt attaagcgtg atatggaaga gcgcgataga
     2281 agtttagata gtattattga acagtataca gaagttgtaa aacctatgta tcatcaattt
     2341 atcgaaccaa caaagcgtta tgcggatatt gtaattccag aaggagtaag taatattgtc
     2401 gctattgatt taatcaatac caaagtggct agcatcttaa atgaagcaaa ataaacacaa
     2461 tggtttgtgt ttattttttt gttttatata ggatggttat ttagttttag taactttcaa
     2521 attatgctat aataagtgct atgaacaagt ctaagaaaat cgaaaattat caattattat
     2581 tactacaagc gcaagctcta ttctcagatg aaacaaatgc tcttgccaac ttatcaaatg
     2641 cttcagctat gctaaatgct atgcttccaa attctgtatt tacaggcttt tatttatttg
     2701 atggagaaga gttaattctt ggccctttcc agggtggtgt atcatgtgtg catattactt
     2761 taggaaaagg tgtttgtggt gaatctgcac aaactgctaa gacgctgatc gttgatgatg
     2821 ttacaaagca tgctaactat atctcctgtg attcaaaagc tatgagtgaa atcgtagtac
     2881 ctatgtttaa aaatggcaaa cttctaggag ttctagattt agattcttct ttagtagcag
     2941 attatgatga gattgatcaa gaatacttag aaaaatttgt aggtattcta gtagaacata
     3001 cgatttggaa tttggatatg tttggagttg aaaagtaatg tatcaggccc tttatcggaa
     3061 atatcgtagc caaacatttg atgaaatggt tggacaaagt gttatttcaa ctactttaaa
     3121 gcaagctgtg tccagcaaaa aaattagcca tgcttacctt ttttcaggac ctaggggaac
     3181 tggaaaaaca agtgctgcta aaatatttgc aaaagcgatg aattgcccaa atcaaattaa
     3241 tggcgaacct tgtaatcatt gtgatatttg tcgagatatt acaaatggta gtttagagga
     3301 tgtcattgaa attgatgctg cgtcaaataa tggtgtcgat gaaattcgtg atatcaggga
     3361 caaatcaact tatgctccaa gtagggcaac ctataaagtt tatattattg atgaggttca
     3421 tatgttgtct acaggtgcct tcaatgcact attgaaaaca cttgaagagc caacagaaaa
     3481 tgttgttttt atattagcaa caacagaact ccataaaatc cctgcaacaa tcttatctcg
     3541 tgtccagcgc tttgaattta aagcgattaa attattagct attcgagatc atttagcaca
     3601 gattttagat aaagaagcta tttcatatga tttggatgct ctgacactag tcgctagacg
     3661 agccgaaggt ggtatgcgtg atgctttatc aattctcgat caagccttga gtttagcgaa
     3721 agataaccac atttctttag atgtagcaga agaaattaca gggagcattt ccttatctgc
     3781 tatagatgat tatgtatcga atatattggc tcacgataca actgaagcac tcgctaaact
     3841 tgaagtaatc tttgatagtg gcaaaagtat gagtcgtttt gcaacagatt tattgatgta
     3901 cttaagagat ttacttgttg ttcaagcagg tggtgaagat agtcattcaa gtgatacttt
     3961 tatagccaat cttaatgtta aacaagatat tttatttgaa atgattgata aagtgacatc
     4021 tgttcttcct gagataaaaa atggtagcca tcctaaagtt tatgcagaga tgatgaccat
     4081 ccaattgtct gaaatggtag aaaaaaattc gtcaaacata cccgcagatg tgacagcaga
     4141 attagatagt cttcgtcgag agttaaaatc gttaaaaaac gaaatgagcc aactctctcg
     4201 agcagatcaa agttcatcaa ctcaaaaagt aaaggttaat aataagacat tcacatttaa
     4261 agttgaccgt acaaagattt tgaccattat ggaagaaacg gttgtagata gtcaacgttc
     4321 acgtgaatac ttagaagctt taaagagcgc ttggaacgag atattagata atattacggc
     4381 tcaagataga gccttattaa tgggatctga acctgtctta gctaatagcg agaatgctat
     4441 tttggcattt gatgcggcct ttaatgctga acaagctatg aaacgtaccg atctcaatga
     4501 tatatttggt aatattatga gtaaagcagc aggtttttca cctaatattt tagctgtacc
     4561 tagaaatgat tttaatcaaa ttcggagcga ttttgcaaag aaaatgaaag ctcaaaagac
     4621 ggaaacagaa cctgaagtta atcatcaaat tccagaagac ttttcttatc tagcagaacg
     4681 aatagcaatc gttgaagatt aaaacgtaac taaaaatagt tgcctgttac aggcaactat
     4741 ttttttgata agttttttaa ttatttagta gattctgtta taatggtgat atgacaaaac
     4801 gacaatttat ttttatggcg cttttatgct cttttgaaac ttattttttt aatcaatccg
     4861 ttatggatgg ctcctggatt tttgctattt tttggggagt tttattactt cgagatcttc
     4921 aaaaggtgta tgcgatttca aaattcacaa aagagcttat caaatctacc aagaagaaag
     4981 actgatttcg ccactgcgaa gtgttttcaa ctgaccatca tctaactcaa ccactaaata
     5041 tcctttatct gtaatatcaa tagctttccc agaatacatt gtttgaccat ccataaagct
     5101 aacggttcta tctaagacta gggatttttc tctatatacc tttaagtgat cctctagtgg
     5161 aatattgaaa aagagattcc agatttctgt aatcaattga tttctagtta tggtcggttg
     5221 ctctgtaaag agactacctg cgcgcttagt taacgcccta gggaactctt tgatatagaa
     5281 attgatacct aatcctataa taacattcgt taccaaccca ctctcaacag aagcgatagc
     5341 ctcggttaaa ataccagcta tttttttatt atctaaataa atatcattga cccacttaat
     5401 ttcaggagta attcctgtta accgtgagat agcttttaca gcactagagg ctaccatgac
     5461 agtataaggt tttatatctt caatcggtac attaggttgt aataaaagag acatatagat
     5521 gccgccttga tttgatgtaa agaaaggtct atcaaaacgt cctttagctt ttttttgatt
     5581 aggagctaaa aaaaggtgtg gagtctttaa tttactttct atccccattt tagcatctag
     5641 ttgggttgaa gcactattat tattaagact aaccttaata ccaatttctt gttcgagttg
     5701 ttctggtaat aatatgtcac ctgataccaa tcggtagccc ttgttagtga cagactcaat
     5761 ctctaggcct aattcttcta acgtttttat acctttccaa atagctgtgc gggaaatatt
     5821 aagttgattt gccatagttt ctccgctaat aaagttgtta ttttttgata gtatttgata
     5881 aattttttcg taggttttca taaatctagt atagtataaa tcgagaaaaa agactaaaga
     5941 aaatagagat atttttttat ttattggtaa cttatttcac aaaagatgaa atagtggtac
     6001 aatagctata tgagtcccga aaaggtagcc tagaggctag ttctttgtaa ctcgacacat
     6061 tttacatttt aggggggaca tttttatgtc agaacgtaaa cttttcacgt ctgagtcagt
     6121 atctgaggga catcctgata agattgctga ccaaatttcc gatgcgattt tagatgcaat
     6181 tttagagcaa gatccagatg cccacgtagc agccgaaaca gctgtttata cagggtcggt
     6241 tcatgtattt ggcgaaatat caacgacagc ctatgttgat attaaccgag ttgtccgtaa
     6301 cacaatcgct gaaattggct atgataaagc tgaatatggc ttctcagctg aatctgttgg
     6361 agtgcatccg tcactcgttg aacagtcgcc agacattgca cagggagtca atgaagctct
     6421 tgaggtacgt ggttctcttg aacaagatcc tttagattta attggtgcag gtgaccaagg
     6481 tttgatgttt gggtttgctg ttgatgaaac acctgagtta atgccattgc caatttcttt
     6541 agctcatcag ttagttaaga aattgacaga tctacgtaag tctggggaac tcacttatct
     6601 tcgaccagat gcaaaatctc aagtaacggt tgaatatgat gaaaatgatc aaccaattcg
     6661 tgtggatgca gtagttattt cgacacagca tgatcctaat gtaactaatg accaactcca
     6721 taaggacgtg attgagaaag ttatcaatga agttattcca agccattatt tagatgacca
     6781 aacaaaattc tttatcaatc ctacagggcg ctttgttatt ggaggccctc aaggagattc
     6841 aggactaaca ggtcgtaaga ttattgtaga tacttatggt ggttattctc gtcatggtgg
     6901 tggtgcattt tcaggtaaag atgctactaa agtagatcgt tcagcatctt atgcagctcg
     6961 ttatattgca aaaaatattg ttgcagctga tttagctaaa aaagtagaag tacaacttgc
     7021 ttatgcaatt ggtgttgccc agcctgtctc tgtgcgtgta gatacatttg gaacaggtgt
     7081 tatagcagaa gcagacctag aggcagctgt acgtcaaata tttgatttgc gaccagcagg
     7141 cattattaat atgttggacc tcaaacgacc tatttatcga caaacagcag cctatgggca
     7201 tatgggacgt actgatatcg atttgccatg ggagcgagtt gataaagtcc aggccttaaa
     7261 ggattttatt gctagcaaat aataaaaagc gtaaacgaaa gtttacgctt tttttgctca
     7321 tgataattta tagaacattt ataaaatctt ataataaact ggttaagtat aggaaatact
     7381 gcatatttct tgaaaatatg gtgtatattg tgaataaaat gatggccaag ttaattgaat
     7441 tttcctatcg aaaaattttt caaaaaaata atttcacact caaatcattt gattatcaaa
     7501 taaatatagc ctttataaaa atattatata agtataaaat gtaaaaaaaa taaaaaaatg
     7561 atatttttat ttgatttaaa tgtatttaa
//
LOCUS       DATRSD010000082         7582 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRSD010000082 DATRSD010000000
VERSION     DATRSD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.083"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..592)
                     /locus_tag="VBD85_001665"
     CDS             complement(<1..592)
                     /locus_tag="VBD85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO7134207.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALLWELMSPEETQISQELSED
                     INATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELY"
     gene            complement(672..1709)
                     /locus_tag="VBD85_001666"
     CDS             complement(672..1709)
                     /locus_tag="VBD85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO7134208.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEDYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRF
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(1763..2491)
                     /locus_tag="VBD85_001667"
     CDS             complement(1763..2491)
                     /locus_tag="VBD85_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7134209.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKDPK
                     KVIESLTPLLKPNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            2667..3659
                     /locus_tag="VBD85_001668"
     CDS             2667..3659
                     /locus_tag="VBD85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7134210.1"
                     /translation="MKNYRKLIVLLLLTFFVIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSPKQIILVGESAGGGLALGLADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(3718..4662)
                     /locus_tag="VBD85_001669"
     CDS             complement(3718..4662)
                     /locus_tag="VBD85_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7134211.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVMMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(4673..5482)
                     /locus_tag="VBD85_001670"
     CDS             complement(4673..5482)
                     /locus_tag="VBD85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7134212.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(5608..7557)
                     /locus_tag="VBD85_001671"
     CDS             complement(5608..7557)
                     /locus_tag="VBD85_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7134213.1"
                     /translation="MNGDVLSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIAD
                     IKLEEAEERRHFNGFDYRQYLKRHGIYRIAKVTKIKQIRLFQHRSFFALMSKWRRSAI
                     VISQTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRY
                     ICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLL
                     ICLISLPHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYF
                     SGFQPISIILTALLSFAFDIIFLPLLTVIFVLSPIVKLSCINSLFEILEVLLKWTGQL
                     FPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVA
                     ILDVGQGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKVNQSNAKRTLIPYLKSRGI
                     SKIDDLVITHTDTDHMGDMEVISKHFKVARLITSSGSLTNSQYVKHLSKIGVAVKSIE
                     AGDKLAVMGSYLQVLYPWHKGDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAY
                     PNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKS
                     KIFRTDQSGTIRLTGWWKWHIQTVR"
BASE COUNT         2543 a         1441 c         1117 g         2481 t
ORIGIN      
        1 ggtaaagttc atttacttta ttccagttaa taatttcaaa gaaagctttg atgtagttag
       61 gacgaacatt acgatagttt aagtaataag catgctccca tacatcaagc cctaaaatag
      121 gtttcttgcc ttccataatt ggagtatctt gattggcagt tgaaagcact tcaagtttgc
      181 cttcagcatt aacaacaagc caagcccaac ctgaaccaaa acgtcctgtt gctgctgctg
      241 tgaaagcagc tttaaagtct tcaaatgaac caaaagttgc attaatgtct tcagataact
      301 cttgtgaaat ttgagtttct tctggtgaca tcaattccca taaaagagcg tggttaagat
      361 gtccaccacc gttattgatg actgcctgac gaatatcttc tggaatttga gaaacatcag
      421 ctaagagcgc ctctaagtct tctccaattt caggatgttt ctcaagagca gcatttgcat
      481 tagcaacata agttgcatgg tgcttatcat gatgtagtgt cattgtctca gcatcaatat
      541 gtggctcaag cgcatcatat gcatagggta aatctggtaa aataattgcc atgtcgaaat
      601 cctcttttca tctattatta cccttattct ataggaattt ggaacagctt tcaactcttt
      661 tgtttcttta attagggctt tttatgagca atttttaata aggctaaatc aaagagataa
      721 tccttatcat aagtccctct tttaatagca tagtcagtct cgattaaaat cctaatcgct
      781 tcttttaaaa aagcaagggg aaggtttcta gaatctctga cagcaaattt tacttgataa
      841 gggttgattt tacgtcctat gtaatgggat agttcagaaa caatttgact ctctgacttc
      901 cctttactag ccaatatttt aacttgcaaa aacatccgaa actgtcctaa catgattgca
      961 atcaatttga tttcgtcttc gccttgtaga cgtaaatctc ttactaaatc acgcgctaaa
     1021 tctatcctgc caagtaagac atcttgagtc aaatcaaata tattatcttg caggctttta
     1081 gggatagcct ctctaacatc attggacgaa atatgaccgt ctgttttata tgactttaag
     1141 aaagcgatat tcgttaaagt atcactaaaa tcatagttag acttaatcaa taactcatca
     1201 aaaacacctg cttcaaaaac taatccttct tggtgagcat atttttgaaa atacgtcttt
     1261 aaatctgatt cttttaaagt attagcctct aaaacacgag catcacgttt caataacttt
     1321 acaagtcgac gttttccatc aagttttcct ggagcacaaa taacaagtct cgtagtatct
     1381 acaggattct gcaagtaggc ttcaaagcgc ttcattgctt gttcatctaa ataagttttt
     1441 ttatcagttg taatatcttg aaactgatca aaaataacaa ccttgtagtc tgataaaaaa
     1501 ggtaaacttt ctaagtctaa ttccgcattt tgatagtctt cttcacttaa atcaaaataa
     1561 gaataggcta aatcgtcttt attgaatcca attacctgaa atagcttctc tttcatctga
     1621 gcatattgcc ctaaatcttc tcctgctaaa acagttacta accctaaatt atctggggta
     1681 attctcccaa tttcttcaat cgcaatcata ttccttatta taccataagg aaacttttca
     1741 aggtgtcaaa taatacttaa aatcattcat caactctatg agaaataatt ccaaattgac
     1801 aatctcggat aaaatggtca ttattctctt ctaattcttt ttgtaaattg acatcttcta
     1861 atccctgaag gtcactatat ttttgtattc tttctttcat gatatttagt agccaagata
     1921 aatctgtacc agtttgtgac gtttgaatga cattttctga tacaaaatgg tcaacgatta
     1981 agcctgcttc taacattaat tgctcaagtt gacttccaat atgaatgtgt cctccttcct
     2041 tctcaacagt ttgccagatt agtgattgta ttttattcat atttacaaag ctaggcttag
     2101 ctagttctaa acacattgca tcactttctt gaaaaacata aaaaccattt ggctttaaca
     2161 aaggtgttaa actttctatt actttctttg gatctttttg atacattaat acacgacgtc
     2221 caataattgc atcaaaactt tttgtttcaa aggaggaaag tgctgctaaa tctgcgtttt
     2281 gataactaac attagcatag ccattatcat gtctagcttg ccataaaagt tgctcattaa
     2341 tatcaattcc tacaacatca ccttctttac caacgatatc agcagccaaa cgcgtcaatt
     2401 ccccactacc acaaccaata tccataactc tcataccggg ctgcaaagct ttttttaata
     2461 atgcttttga aaaatcttct ctgtgaaaca taataacact ctcctctctt ctattttagc
     2521 aaattaaagg ttaatacgta ctatcagcaa aaaattataa taggttgaaa ttttatagca
     2581 ttattttctt gttttcgtat ctactttatt atagaataac aactaatcat ttttagcatg
     2641 acgagctcaa ttacaaaagg ataatcatga aaaactatcg aaaacttatt gtactactac
     2701 ttctaacctt ttttgtcatt tttatgggag catatgctta cacgcatatt gttgaaaaaa
     2761 gatccctaac tagcaatact attgaaaaaa ctctacctgt ggtaaatcag attaagcctc
     2821 aaaccatcaa agaataccaa aattacttaa ctaaggtagc taaacgtaat gttcttcctg
     2881 tagacattcc tcaggcatta aataatgaaa aggtagaaat taccgctact gatggcatgc
     2941 aaacattcac ttggaatgat aaaaataatc ctaagcaaaa ggttatcttc tatgttcatg
     3001 gaggatcata tatccatcaa gcttctgaat tacaatatat ttttgtcaat aaactagcta
     3061 aaaaattaga tgcaaaagtt gtctttccta tttaccctaa agctcctaca tataattata
     3121 gtgatgctat ccccaaaatt aaaaaattat accaaaatac attagctagc gtcacatctc
     3181 ccaaacaaat tatcctagta ggtgaaagtg caggcggagg tcttgcttta ggtcttgctg
     3241 ataaccttgt cacggagcat atcaaacaac caaaagaaat tattttaata tcaccatggt
     3301 tagatattgc tactaataat ccaaagattg aaaaagttca aaaaaaagat ccccttctta
     3361 aagcttggca attacaacaa gtagctcctt actgggcaaa tggaaaaaag aattttaaaa
     3421 atcctcaagt tagtcctctt tacagtagcc aatttaacaa gatggctcca atatcatttt
     3481 ttataggaac gcacgatatt ttctaccccg ataatcaatt attacatcaa aaattagcaa
     3541 aagaaaacat taaacaccac tatatcgttg gtcaaaaaat gaaccacgtt tatccagtct
     3601 taccgattcc tgaagcagag actgctttca aacaaatgct taaaattatc aatcattaaa
     3661 taaaaaggtt tccaatcaag tttagttgga aacctttttt ctattacttt tgaatgacta
     3721 attgtcttga gataaaaact ctgagaataa aacttcttga ctaatctttt cagctaaagc
     3781 ctgtttaaaa gctaatttcc ctaaataata ggatttgtgg tcaattgttg ggagtttcaa
     3841 aagttgccca actaataaac attcctgacc aataatataa ggacattttt tcctactttc
     3901 ttcataacct tttattagcc ctgccgcaac ttcatcactt gtagctaata taccatctat
     3961 actaacttct tttaccaatt gataagataa atttagtcca tcattaaagt catgaacatt
     4021 cccaactacc atatatggag aggatagttg cccatagacc tcttgataag ctaataatgc
     4081 ggattgatag gtagcagagg attcattatt ccttgaaaat aataaaacta agtgctccaa
     4141 acctcgtaac ttcatatcag aaaacgcctc tagaaatgaa gaatagcgat caaggtaagc
     4201 agaggataga tgattatatt cctgcaattt ttcacaaact actattcgtc catattttgc
     4261 ataagtttca ataatatcga ggctaattgc tcttgaagtg aatattaaag catcaatagc
     4321 ttccatcttg agctgtttca agtaggaaag ttctaattct tggttgtaat ccgaaggcat
     4381 cataacaagt tgatagtcgg tagttttagc agcatctaat aggccattta ttaattgtgt
     4441 aaaataagga tggcgcgtgt ggggaatgac gacacctatt ttttgtgttt tgcctcgact
     4501 taaatcccgc gctagttgat ttggtatata atccaattcg gtgataagag ccattacttt
     4561 ttgtcgcgtc tcatcagaaa catgtgggtg atgattgata acacgggaaa ctgttgattt
     4621 agcaacacct gcctttttag ctatatcact aatacttgtc attataaggc tcctacttta
     4681 aaaatttttg aatcccatat cccactccgt cttcatcatt agacttagtt agttgatatg
     4741 ctatcgcttt gatatcatca aaagcattat ccataacaat agggtaacct accatttcaa
     4801 gcataggcaa atcattatgt ccatctccaa atgcagccgt ttcttctcta gctaactgct
     4861 ctttttgaag gatgtaagca attcctttag attttttggc taagagatgt gtaatttcaa
     4921 gatacgcttt acctgaacgt tgaatagtga tttctggtaa ctccaaactt tgaagatatt
     4981 tttctaattc tagcatattt gcttcatcga aagtaatcat cataatttta aaagtatttg
     5041 tgtgaccttc taaaaactga tcctcattat gaataaacgt tgggcattgt tgagttaaac
     5101 tatgttcgta tcggattcct tcatctattt tatcacaata ccagttatta agatcgtaat
     5161 aagataaact aacttgagga aaatgaaacc gtattcctct tagcaattgt tttactgtgc
     5221 tttttttaat gatttgtgta tgaataggta gaacttgatt attatctcct attctataaa
     5281 tcaatcctcc attaaaagca acttggactc ctcctaattg aagtgcatct acggcatcct
     5341 tcatttccat aggtgcccta gcagacacta aagttactgg aatcgctgcc tcacgtatta
     5401 aggtagcgtt acttttactc acttgtcctt cactatttaa tagtgtacca tccatgtcac
     5461 aaaaaatacg tttgatagtc ataaaaaata cctctcttct tctgaaatca gtataaatgc
     5521 tggaacccgt tccatgtcaa gcttaaaatt actgaattct tgcgaaaaat agattgtatt
     5581 taacagatac gcaatcattt ttataaatca acgaactgtc tgaatatgcc acttccacca
     5641 tcctgttagc ctaattgtac ctgattgatc cgttcggaaa atcttgcttt taatcttttg
     5701 gaacctttgt aaagtctctt gatgaggatg ctggtaacga ttatttttac cagctgaaac
     5761 tagaaccaca ctaggagaca actttttcag aaaggataga cttgatgagc ccttagaacc
     5821 atgatgtcct gctttaagga tatctactga taaattagga taagcttcta ataactgctt
     5881 ttctccctct tcctccaaat caccggtaaa taagaagcct tttcctaata aatgtccata
     5941 taaaacaatt gaatcattat tttttccatc acccttgtgc catgggtaaa gtacttgtaa
     6001 ataacttccc atgacagcaa gtttatcacc ggcttctata gattttaccg ctacacctat
     6061 ctttgataaa tgcttaacgt actgcgaatt cgttaaagaa cctgaacttg taatcaaacg
     6121 tgcaacttta aaatgctttg agataacttc catatccccc atatgatctg tatcggtatg
     6181 agttatcact aaatcatcta tcttgctaat acctctgctt ttcaagtaag gaatgagcgt
     6241 tctcttagca ttagactgat ttactttttg tttccattct tcaggctgtt caaatctcac
     6301 ccttccccca gtatcaatta aaattgtttt tcctaaccaa tccctcacta aaatactatc
     6361 tccctgtcca acatctaaaa tcgcaacctc gttagtaatt ggattcttag tgataaaaaa
     6421 caaggtaaag ataataagaa gggagcaata acgaaaacat ttagaatgat aataatcata
     6481 aagtaatccc aaaattataa tcatgactat taaaagaaaa aggctgggct ttccaaaaat
     6541 aagtggcctt ggaaacagtt gcccagtcca ttttaataac acttctagga tttcaaacaa
     6601 actattaata caacttaatt taacgatagg cgataagaca aatataacag ttaataaagg
     6661 caaaaatata atatcaaatg caaaagataa aagtgctgtt aatataattg atattggttg
     6721 aaaacccgaa aaatagtagg ttagtatggg aagaattcct acagagactg tcaaagattc
     6781 gatagctact ttttttatac tcgaaaaatg atcaaaggag gtcatcgtaa gtataaaagc
     6841 ataagcaaag ctaagaactc ctcccgtagt caaaagtgag tgggggaggg agataagaca
     6901 aattaacaag cagagagcaa agttctcatc tttcttgaca ccgctatgtg ataataaaga
     6961 ttgaataagt gccctaacga ctgagatact aaagcctgtt aaaccagcat aaattagcga
     7021 gaatggtatt tgaagtaacc aaacatggtc tagacgtaag ccaatacgta gacagatata
     7081 acgaaaaata ccgagaaaaa aacctacttg catacctgac aaagcaaata aatgtataat
     7141 acctagacta ctatataaat cggacatgtc atcaaaggtc ttatctagat atccaaacaa
     7201 aagccctgac atatagtgac gcataggatt tggaaaagtt tgactaataa caattgcact
     7261 tctacgccac ttagacataa gagcaaagaa agacctatgt tgaaataagc gtatctgttt
     7321 tatctttgtc actttggcga tacgataaat tccatgtctt tttaaatact gacgataatc
     7381 aaagccatta aaatgccttc tctcctctgc ttcttcaagt ttgatatctg caattatcat
     7441 tatattatta tcaagatttt gaaaataatc tttctcactt ttatttttta atgtgtaaaa
     7501 agcttgataa gtgttacctg aagcatctgc ctggaatgat aaaacatcac cattgataga
     7561 aatagtatca atacgcactt ta
//
LOCUS       DATRSD010000083         7514 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRSD010000083 DATRSD010000000
VERSION     DATRSD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.084"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            71..1795
                     /locus_tag="VBD85_001672"
     CDS             71..1795
                     /locus_tag="VBD85_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134214.1"
                     /translation="MRKYLQKKFQLTEAGAKGLEKSVWSSFAYYAIYMLPMILMMFFV
                     QSILENKGHGLETYVLGIGIIIILVYVATNMNYKTTYNETYKESANLRIEIADILKKL
                     PLDYFSKHDISDLSQTVMADVAAIEHALAHAIGHTIGYVYFFIVIGVMMLIGDFKLGI
                     CVLLPLIISAGILYLSKKSQIKVRGNHYEKLREISEEFQSSIEMSQEIKSYGLKENTI
                     ENISKKLDESEKIQMRAEIVQAFPVSLAGWITKLSIGITSVVGLHMFISNEVPLLYLV
                     GYIIAATKISEGLEGIYMYLGEIFYLDARINRIKELRNTKAQIGNNVDFDNYDISFQN
                     VDFSYSGKNKKVINNVTFTAKQNQITALIGPSGCGKSTLLKLMSRLYDYDSGRILIGG
                     HDIKTVDTECLFKKISVVFQDVVLFNTSIMENIRIGNTNATDDEVREVARIAGCQEII
                     DRLPEGIQTIVGENGAKLSGGERQRISIARAILKDAPIILLDEIAASLDVENEAKIQE
                     GLNKLIKNKTVVIISHRLKSIQNVNQIILLDEGKVVSNGTHDEIYNESQLYRNMIEKS
                     DLTEMYSY"
     gene            2194..2610
                     /locus_tag="VBD85_001673"
     CDS             2194..2610
                     /locus_tag="VBD85_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7134215.1"
                     /translation="MAKEYYLYVNGQRVKVSEQIYKVYWREKEHEKYLEQVDKKNHLL
                     FFSSLDHDGNFVDNLADESVDVEKIIETQILIETVRKAMSRLNDEERDIIERLYFNDE
                     TLSSVARSKKVSYQAIQWRKNNILKKLKVLLKEFIK"
     gene            3059..3205
                     /locus_tag="VBD85_001674"
     CDS             3059..3205
                     /locus_tag="VBD85_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134216.1"
                     /translation="MNNELLKYSLQLSMLSMLLSKHLLSDIEYKKIKLKLMKKYNIST
                     ELYL"
     gene            3265..5070
                     /locus_tag="VBD85_001675"
     CDS             3265..5070
                     /locus_tag="VBD85_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO7134217.1"
                     /translation="MKKDVKVIKGDTTLKRTASGCVQRSIKRVAAYCRVSTDTEDQIN
                     SYNSQVEHYTEFIQKNKEWTLAGIYADEAITGTQVDRRIDFQRLINDCMNGDIDMIIT
                     KSISRFARNTLDTLKYVRKLKEFNVAVFFEEENINTLTMDGELLLTILSSVAQQEVEN
                     ISANVKKGLRMKMERGEMVGFQGCLGYDYDPETKSISINEKEAEIVRYIFRRYIEGVG
                     GMVISRELEEQGYLSPRGNKRWTETTVLGIIKNEKYKGDLMMGKTYTVDPISKRRLDN
                     FGEQDKFYIENHHEPIISEEDFEKAQGIRLRRSKNRNTVANNGGKREKYSRKYAFSSM
                     LECGFCGHNLSRRNWHSSSEYTKVIWQCVNATKNGKKYCPHSKGIEEEAIEKAFMESY
                     RRVCHNNVEITNEFLKTVEEELKDNSLAKDLKKITNQLDKILKKEKDLVELRLNESIS
                     MDIYQDKYNEIAISKEKLLAEKRTLEVTLTDEKALKKRLEGFKKLLESNKYLEEFDRA
                     VFESIVDKIIIGGTNDEGEIDPAMITIIYKTGKKDSQDGRLFKSRRKNAKEVNEETDN
                     KLYPHSSDEVNNLYSYPIDNTCGVCGSARKSLETF"
     gene            5167..5388
                     /locus_tag="VBD85_001676"
     CDS             5167..5388
                     /locus_tag="VBD85_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134218.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            5506..6243
                     /gene="aphA"
                     /locus_tag="VBD85_001677"
     CDS             5506..6243
                     /gene="aphA"
                     /locus_tag="VBD85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO7134219.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            6564..7082
                     /locus_tag="VBD85_001678"
     CDS             6564..7082
                     /locus_tag="VBD85_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO7134220.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AANLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(7211..7514)
                     /locus_tag="VBD85_001679"
                     /pseudo
     CDS             complement(7211..7514)
                     /locus_tag="VBD85_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445921.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
BASE COUNT         2862 a          955 c         1463 g         2234 t
ORIGIN      
        1 tgaatttaaa tggaagatat agagaacttc aaaacttgta tgaaaaagca aatgattgga
       61 gggtaatgta atgagaaaat atttacaaaa gaaatttcaa ttaactgaag ccggagcaaa
      121 aggtctggaa aaatccgtat ggtcttcatt tgcatattat gctatttata tgcttccaat
      181 gattttaatg atgttttttg tgcaatctat tttagaaaat aaagggcatg gacttgaaac
      241 ttatgtttta ggaattggga taatcataat acttgtttat gtggcaacaa atatgaatta
      301 taaaaccaca tataacgaaa cttataaaga atctgccaat ttaagaatcg aaatagcgga
      361 tatactaaaa aaattgccat tagattattt ttcaaaacat gatatctcgg atctttcaca
      421 gacagttatg gcagatgttg cggcaattga acatgcttta gctcacgcaa taggtcatac
      481 aattggctat gtttactttt tcattgtgat aggcgtgatg atgttaatag gagattttaa
      541 acttggaatt tgtgtgttac ttccactgat tatttcagca ggtattttat atttatcaaa
      601 aaaaagccaa ataaaggtga gaggaaatca ttacgaaaaa ttaagagaga tttcagaaga
      661 atttcaatca tctattgaaa tgagtcaaga aataaaaagt tatggattaa aggaaaatac
      721 gatagaaaac atttctaaaa agcttgacga atccgaaaaa attcaaatga gagcagaaat
      781 tgttcaagct ttcccagtaa gcttggctgg atggatcaca aagttatcta taggtattac
      841 aagcgttgtt ggactccata tgtttataag taatgaggtg cctttgttat atttagttgg
      901 atatattatt gcagcaacta aaatctctga aggtttagaa ggtatttata tgtatcttgg
      961 agaaattttt tatcttgatg caagaattaa tagaattaaa gagttaagaa atactaaagc
     1021 ccaaattgga aataatgtag attttgataa ttatgatatt agttttcaaa atgtggattt
     1081 ctcatattca gggaaaaata agaaagttat caataatgtt acttttacag caaaacagaa
     1141 ccaaattact gcacttattg gaccatcagg ttgtggaaaa tcaacattat taaaattgat
     1201 gtcaagactt tacgattatg atagcggtcg gattttaatt gggggtcatg atattaaaac
     1261 agtagatact gagtgcttat ttaaaaaaat ttcagtcgta tttcaagatg ttgtgctgtt
     1321 taacacttct attatggaaa atatacgcat aggaaatacg aatgcaacgg acgatgaagt
     1381 gagagaagta gctcgaattg ccggttgtca agaaattata gacaggcttc ctgagggcat
     1441 acaaacaatt gtgggagaaa atggggcaaa attatcagga ggtgaaagac agagaatttc
     1501 aattgcaaga gccattttga aagatgcacc aattattctt cttgacgaaa ttgcagcttc
     1561 attggatgta gaaaatgaag caaaaattca agaaggatta aataaactta taaagaataa
     1621 aacggttgtc attatttcac atagattaaa atctattcaa aatgtaaacc agataatttt
     1681 gcttgatgag ggcaaagttg tgtcaaatgg tacacatgat gaaatttata atgaatcaca
     1741 gttatataga aatatgattg aaaaatctga tttgactgaa atgtattcgt attaggagaa
     1801 atagttaaat atgaaagaac aattaatagc aaaagaccga tagatttatt ctttcagtta
     1861 tctattccag cagtaataac aatgatagtt attggtcttt atccacttat ttatggagta
     1921 cttatgatgc ttatatttat ctataatgaa cggagggaaa gggagatgaa caagtgtata
     1981 agttaccttg tagaattgaa atggaataaa aatactcctt ttttctataa ttcttgtagt
     2041 tgtctgtctt tatatattct cgtctaaaca attaaatata gtaaaagaaa gaacggattt
     2101 ctttcatttc agaggacttc ttatgccaag atgcaggagt cctccttttt tgtctgaata
     2161 acaaatagac aaaaaagaaa tggaggaaac tttatggcaa aagagtatta cctttatgtc
     2221 aacggacaaa gggttaaagt cagcgagcag atatataaag tctactggcg agaaaaagaa
     2281 cacgaaaagt atttggagca ggtggacaag aaaaaccact tgctcttttt ttcatcatta
     2341 gatcatgacg ggaattttgt agataacctt gctgatgaaa gtgtggatgt agaaaaaata
     2401 attgaaacac agatcttaat tgaaacagtc agaaaggcta tgtcaagact gaatgatgaa
     2461 gaaagggaca tcatagagcg tttgtatttt aatgatgaaa cattatcgag tgtagcaaga
     2521 agtaagaaag taagctatca agctatacaa tggcgaaaaa acaatattct taaaaagcta
     2581 aaggtgcttt tgaaagaatt tataaagtaa acgccttgta gatgggggca gattttcctt
     2641 tataaattga agggagatct gtccttatat tttatttaag gaagtgatga aaacaataaa
     2701 gttgaagaga atctctctta aagccgttag gctattgttc cttgaaaact gaatagacca
     2761 aggtaggact ttatctactt gtggaggatt atgacttacg ccatgacctt tctgctgaga
     2821 acaatttcgg tttaatgaat actgagcaag tcaaggaaac aagaaagcga gcgataaata
     2881 agaatacata aaaacagatt tggcaacctg ttcaatgaag caagtagtga taatgatact
     2941 tcaatagttt aagccggctc gtctggaata agaggcggag gtgagattcc tatgttgctg
     3001 ccaagcacct accaatgtca ttaaacttaa aaataaattt gaatgaggag gagttgaaat
     3061 gaataatgaa ttattaaaat acagcctcca attatctatg ctgtctatgt tactttctaa
     3121 gcacttgctc agtgatattg aatataaaaa gataaaacta aagctgatga aaaagtataa
     3181 tatctctaca gaattatatt tataatgttt tgcaagtgtg gtataataga actgcataga
     3241 aagatacaaa aaggaggcgg ttcagtgaaa aaggatgtta aagttattaa aggtgatacc
     3301 acactgaaaa gaactgcatc agggtgtgtt caacgctcaa taaagagagt ggcagcttat
     3361 tgcagagtta gtacggatac tgaagatcag attaatagct ataattctca ggtagaacat
     3421 tacacagaat ttatacagaa aaataaagag tggactcttg ccggaatata tgctgacgag
     3481 gcgataacag gaacacaggt tgatcgaaga attgactttc aaagattaat aaatgactgt
     3541 atgaacggcg atatagatat gataattaca aagtctatat caagatttgc aagaaacaca
     3601 ttagacacct taaagtatgt aagaaagttg aaagagttta atgttgcggt cttttttgaa
     3661 gaggaaaaca taaacacctt aacaatggac ggagagttgc ttttaacaat tttaagttca
     3721 gttgcgcaac aggaagtaga gaacatttca gccaatgtca aaaaaggttt gagaatgaag
     3781 atggaaagag gagaaatggt aggctttcaa gggtgtttag gctatgacta tgatccggaa
     3841 actaaaagca tttctatcaa tgaaaaagaa gctgagattg taagatatat tttcagaaga
     3901 tatattgaag gtgttggcgg tatggtaatc tccagagaac ttgaagaaca aggatattta
     3961 tcgccaagag gaaacaaaag atggacggaa acaacagttt taggaatcat aaaaaatgag
     4021 aaatataaag gggatcttat gatgggaaag acctatacgg ttgatcctat ttcaaagaga
     4081 agattggata atttcggaga acaggataag ttttatatag agaaccatca cgagccaatc
     4141 atttcggaag aagattttga aaaagctcaa ggtattagat taagaaggtc aaaaaacaga
     4201 aataccgttg ctaataatgg cggtaagaga gaaaagtatt caagaaaata tgcttttagc
     4261 agtatgcttg aatgtggatt ttgtggtcat aatctttcaa gaagaaattg gcattcgagt
     4321 tcagagtata caaaagttat atggcagtgt gtcaatgcta ctaaaaacgg aaagaagtat
     4381 tgtccgcata gtaaagggat tgaagaagaa gccatagaaa aagcattcat ggaaagttat
     4441 cgtcgagtat gccacaataa tgttgaaatt actaatgaat tccttaaaac tgtggaagaa
     4501 gaattgaaag acaacagttt agctaaagac ctgaagaaga taacaaatca acttgataag
     4561 atactaaaga aagaaaagga tcttgttgag ttaaggctga atgagtcaat cagcatggat
     4621 atatatcagg ataagtataa tgaaatagct attagtaaag agaaattact tgcagaaaaa
     4681 aggactttag aagtaacact cactgatgaa aaagcgttga agaaaaggct cgaggggttt
     4741 aaaaaattac ttgagtccaa taaatatctt gaggaatttg atagggcagt atttgaaagt
     4801 atagtagata aaatcatcat tgggggaact aacgatgaag gtgaaattga tcctgcaatg
     4861 attactatca tatataagac tggaaaaaaa gattctcagg acggaagatt attcaagagc
     4921 agaagaaaaa atgccaagga agttaatgag gaaactgaca acaaattgta tcctcattca
     4981 agcgacgagg ttaataattt gtattcctat cctattgaca acacatgtgg agtgtgtggc
     5041 tctgctcgta aaagcctaga aactttttaa cgaaaggaat attaaaaagc cttagataat
     5101 cgactgacct attaccaaaa aatcgaaggg gctctacttg ccattgcaca aacaccagga
     5161 aatagtttgc caactatgca gctgttagat gagttagttc ttccaaaaga cattaccata
     5221 gcagatgtca gtcaaagtat ccaagaattt gaaatagaaa gagatgccct tcaagaattt
     5281 tttgacgata tgctgaagac ttatacaacc taccaagaaa tgaaagatta tattaaatct
     5341 cgtgaagcga acactcctaa gcaagagaca gaagaaaaat cactttgaag actgtagact
     5401 taacataagt aaaaaataac catatatgat atcgctttca ttcttatgtt ttttgtgata
     5461 aactaattaa agaaataatt tttatacttt attatggagg ttcttatgaa gaaagtgtta
     5521 gtgagtagtc ttttggtttt agggattacg ataacgttac aaacagtagt tgaggctaag
     5581 gggccaaaag tagcttatac acaagaggga atgactgctc tttcggacac aaataaagat
     5641 aaagtcacta ctatttctat tgacgagatt caaaaaagct tagaaggtaa gaagccgatt
     5701 actgttagtt ttgatattga tgatacactg cttttcagta gtcaatattt tcaatatggt
     5761 aaagaatatg taactcctgg atcgtttgat tttcttcata aacaaaaatt ctgggatctt
     5821 gttgcaaaac gaggagatca agattccatt cccaaagaat atgctaaaaa attaattgct
     5881 atgcatcaaa aacgaggaga taaaattgtt tttataacag gtaggacaag agggtcaatg
     5941 tataaggagg gcgaggttga taaaacagct aaagccttag ctaaagattt taaattagac
     6001 aaaccaattg ctgtaaatta tacaggcgat aaacctaaaa agccatacaa atatgataaa
     6061 tcatattata ttaagaaata tggttcagac attcattatg gagatagtga tgacgatatt
     6121 catgcagcta gggaggccgg tgctagacca attagaattt taagagcacc taattctaca
     6181 aatctacctt taccagaagc tggaggctac ggtgaagagg ttctcgaaaa ttcagcttac
     6241 tagtatcttt attagttaac atagtattat ctatcaattt ttagatttgt aataagaagt
     6301 gctaaaaaag tagcaaatca atgatatgct aataagggaa gttggagtag tttagattga
     6361 ttattaatga ctgatcttct cccctctcgg taacaagaag tttttctaac aaatgcctgt
     6421 ctaaaaaaat tgaatcataa ttttttaata actcttttgg cgtgaacaag gaatttgtaa
     6481 tcagtcatct aattctataa tacaatactt tggggttgtt tccgtatcat tattaataat
     6541 atgtatcaga aaggagatgt tttttgagag tttatgaaaa taaagaagag ttgaaaaaag
     6601 aaataagtaa aacatttgag aaatacatta tggaatttaa taatattcca gagaatctaa
     6661 aagataaaag aattgatgaa gttgatagaa ctccagcagc caacctttct tatcaggttg
     6721 gctggaccaa cttggttctt aaatgggaag aagatgaaag aaagggactt caagtaaaaa
     6781 caccatcgga taaatttaaa tggaatcaac ttggtgaatt atatcagtgg ttcacagata
     6841 cctacgctca tttatctctg caagagttga aagcaaaatt aaatgaaaat attaattcta
     6901 tctatgcaat gattgatttg ttgagtgagg aagaattatt tgaagcgcat atgagaaagt
     6961 gggctgatga agcgactaaa acagcgactt gggaagtgta taagtttatt catgtaaata
     7021 cggttgcacc ttttggaact ttcagaacta aaatcagaaa atggaagaag atagtattat
     7081 aaattatatt tttaacttta aaaaatttca taaaaatggt taccaaaggc gatagaagaa
     7141 aaaactatcg tctttttctt tataaagtat tagatgagga aaagaaatat tattttttct
     7201 aatacaaata ttaaattctc acgacagatg taatatatct tgctatttct tcaggctctt
     7261 cagtaaagtc atgatctatc caatttaaaa taatactttc aattgttgag atataaatac
     7321 caagagcgaa agaatatgga acttgatatg ctgtttccag ttgtttttgt aagtctggta
     7381 tatcggaatg tgtaatcatc tctttgatat agtttttaat tatgttgtca atattcggaa
     7441 aaaacaaagg atttaataat ttctttattt atatacatat agctaagcat ctcctttaag
     7501 accacttctt cata
//
LOCUS       DATRSD010000084         7112 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRSD010000084 DATRSD010000000
VERSION     DATRSD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.085"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            24..3380
                     /locus_tag="VBD85_001680"
     CDS             24..3380
                     /locus_tag="VBD85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEO7134221.1"
                     /translation="MSQKTFGKQLTVVDTKSRVKMHKSGKNWVRTVMSHFNLFKAIKG
                     RATVEADVCIQDVEKEDRLSSGNLTYLKGILAAGALVGGASLTSRVYADETPVVQEQS
                     SSVPTLAEQTEVTVKTTTVQNHQDGTVSKNIIDSNSVSMSESASTSTSESVSMSMSGS
                     TLTSVSESVSTSALTSASESISTSASESVSKSTSISEVSNILETQASLTDKGRESFSA
                     NQIVTESSLVTDAGKNASVSSLIEITKPKSELQTSKMSNESLITPEKSQVMIASDKTG
                     NESLTPTIRLKSVIQPRSMNLMTLSSEMDLIPLEEVSDTEMLGKDVSSELQKVNIALK
                     DNTLSEPGTVKLDSSENLVLNFAFSIASVNEGDVFTVKLSDNLDTQGIGTILKVQDIM
                     DETGQLLATGSYSPLTHNITYTWTRYASTLNNIKARVNMPVWPDQRIISKTTSDKQCF
                     TATLNNQVASIEERVQYNSPSVTEHTNVKTNVRSRIMKLDDERQTETYITQINPEGKE
                     MYFASGLGNLYTIIGSDGTSGSPVNLLNAEVKILKTNSKNLTDSMDQNYDSPEFEDVT
                     SQYSYTNDGSKITIDWKTNSISSTTSYVVLVKIPKQSGVLYSTVSDINQTYGSKYSYG
                     HTNISGDSDANAEIKLLSESASTSASTSASTSASMSASTSASTSASMSASTSASTSAS
                     TSASMSASTSASTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASTSASMS
                     ASTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASTSSSTSASTSASTSAS
                     TSASMSASTSASTSASMSASTSASTSASTSASMSASTSSSTSASMSASTSASMSASTS
                     ASTSASTSASMSASTSSSTSASMSASTSASMSASTSASTSASTSASMSASTSASTSAS
                     MSASTSASTSASMSASTSASTSASTSASTSASTSASMSASTSESISASTSASMSASMS
                     ASTSASTSASTSASTSASMSASTSASTSASTSASMSASTSASTSASMSASTSASTSAS
                     MSASTSASMSATTSASTSVSTSASTSASTSASTSSSSSVTSNSSKEKVYSALPSTGDQ
                     DYSVTATALGLGLMTGATLLGRKKSKKDKD"
     gene            3711..4718
                     /locus_tag="VBD85_001681"
     CDS             3711..4718
                     /locus_tag="VBD85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7134222.1"
                     /translation="MRVHITSIYGQSPRSIALISQKLVKDVGRQLGYDEMGIYFYNDH
                     AETHGERSTRMDGIIAGLGRGDIVVFQVPTWNSTEFDELFLDKLQAYGARIITFVHDI
                     VPLMFESNFYLLDRVIDMYNRSDVVILPTKAMHDYLIEKGMTTSKVLYQEVWDHPVNI
                     DLPRPECQKVLSFAGDIQRFPFVNDWKENIPLIYYGDGSRLNSEANVHAQGWKDDVEL
                     MLSLSKRGGFGLCWSEDREELVERRYSRMNASYKLSTFLAAGLPIIANHDISSRDFIK
                     QHGLGFTVETLEEAVEKINNMEKETYDSYVENVEKIATLLRNGYITKKLLIDAVHMLY
                     R"
     gene            4727..5932
                     /locus_tag="VBD85_001682"
     CDS             4727..5932
                     /locus_tag="VBD85_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7134223.1"
                     /translation="MKKTIVLGADFQYRDQVMTTIKSIVSHNQHLTIYIINTDFPVEW
                     FNILNHSLEQFDCRVKNIPISSDVFEGIPTLSHISVAGFFRWFIPIHLEEEIVLYLDS
                     DVIVRGSLDPLFDINLEENLLGAVADHFSTLYYGDTAPVSFNSGVMLINNSLWKKEEI
                     YNSLMRIADKGSAVGVGDQEYLNILTQNRWIDIGKQYNVQIGQDVNINAYGRPDLYHF
                     YDDCEPVIVHYNSQDKPWNKYSQSRYRSEWWYYFGLEWSVIYAQQQKNLNRLTGKTLN
                     LFTLTASDTLEHIEVLAKALPDYQFHIAARSDISEKLANLETYSNIKVYKLVINPILE
                     ELIRNCDVYLDINHSYEVEGILDKIKEENKPILAFDSTAHTTKNIEVISETMPELMVK
                     RIREVGPLR"
     gene            5929..>7112
                     /locus_tag="VBD85_001683"
     CDS             5929..>7112
                     /locus_tag="VBD85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7134224.1"
                     /translation="MKNRKAIALAADFGYQEQVKTIIKSICFHNQFIDFYILNDDFPV
                     EWFQMMEYHLSKMDCTISNTKIFNEEIKHFKFQKPMPYPTYFRYFIPEVIHEDKVLYL
                     DCDMIITSDLTSIFTLDISKYGVAAVRDDLLEEYDGKEDYFNSGLLLINNIFWREQGI
                     SQRLLDYTRENQGALQYHDQDVLNDVLCDNWLELDETYNYHTGADMLYNLFQQSERQL
                     NRRKDLPKVIHYTATKPWKYLETSVRWRDIWWEYNRLEWRDIFTRWQVNDGVDVSLKK
                     VLQPIHRAFIFTYSDSIEGIERLAKDLPNWQFHIAAYTQISEKLKQLEMHSNIKLYPL
                     VYRFKLNELVENSDIYLDINHGDADTQVLEQVKKLGKDILSLSTTYHGQGKQFERVTD
                     II"
BASE COUNT         2365 a         1250 c         1415 g         2082 t
ORIGIN      
        1 aaatatttat atatggagga aacatgtccc aaaagacttt tggcaagcag ttaacagttg
       61 tagatactaa gagtagagtc aagatgcata aatcaggaaa aaactgggta agaacagtaa
      121 tgtcgcattt taatctattt aaagcgatta aagggagagc aactgttgaa gcagatgtgt
      181 gtattcaaga tgttgaaaaa gaagaccgac tatcttcagg aaatttgacc tatctcaaag
      241 gaatactagc tgctggagct ctggtaggtg gagcgagttt aaccagtcgt gtttatgcag
      301 atgagactcc agttgttcaa gaacaatcaa gttctgtacc aacactggca gaacaaacgg
      361 aagtgactgt taaaacaact actgttcaaa atcatcaaga tgggacagta tcgaaaaaca
      421 ttattgattc taatagtgta tctatgtcag agtcagcctc aacaagtact agtgaatctg
      481 taagtatgtc tatgtcaggg tcaactttaa caagtgtaag tgaatctgta agtacatctg
      541 ctttaacaag tgcttcagaa tcgataagca cgtcagcctc agaaagtgtt tcaaaatcta
      601 caagtattag tgaggtttca aatattcttg aaactcaagc ttctttaact gataaaggaa
      661 gagagtcgtt ttcggcaaac cagatagtaa cagaaagtag cttagttact gatgctggta
      721 aaaatgcttc agtatctagc ctaattgaaa ttacaaaacc aaaatcggag ttacagactt
      781 ccaaaatgtc aaatgagtcg cttataactc cagagaaatc ccaagtaatg attgcaagcg
      841 ataaaactgg gaatgagagt ctaactccga caattagatt aaaatcagtt attcagccaa
      901 ggagtatgaa cttgatgact ttgagttcgg agatggactt gataccacta gaagaagtgt
      961 ctgatactga aatgttaggt aaagatgtat caagcgagtt gcagaaagtt aatattgcgt
     1021 taaaagataa cactcttagt gagcctggaa cagttaaatt agatagttca gaaaaccttg
     1081 ttttgaactt tgccttttca atcgcttctg ttaacgaggg agatgtcttt actgtaaagc
     1141 tttctgataa ccttgacaca caagggattg gtactattct aaaagttcaa gatataatgg
     1201 atgaaacggg gcagttatta gcgactgggt catatagtcc tttaacacat aatattacat
     1261 acacctggac aaggtatgct tctacgttga ataatattaa agctagagtc aatatgccag
     1321 tttggcctga ccagagaata atttctaaaa caacttcaga taagcagtgc tttactgcaa
     1381 cattgaacaa tcaagttgct tcaattgagg aacgtgttca gtataatagt ccttcagtga
     1441 cagaacatac taatgttaag acaaatgtaa gatctcggat catgaagctt gatgatgaaa
     1501 gacagacaga aacttatatt actcaaatta atcctgaagg taaggaaatg tatttcgcat
     1561 caggacttgg gaatctatat actattatcg gttcagatgg aacatcaggt tcaccagtta
     1621 atttattaaa tgcggaagta aagattctaa aaactaattc aaaaaatctt acagatagta
     1681 tggatcaaaa ttatgattcg cctgagtttg aagatgtgac ttcccagtat agttatacta
     1741 acgatggttc taaaattacc atagattgga aaacaaattc tatttcttcc actacatctt
     1801 atgttgtttt ggtcaaaata cctaaacaaa gtggtgtatt gtattcaact gtttctgata
     1861 taaatcaaac atatggttct aaatattctt atgggcatac gaatataagt ggtgactcag
     1921 atgcgaatgc cgaaattaaa cttttatcag aaagtgcttc tacgagtgcg tcgacgtcag
     1981 caagtaccag cgcttccatg agtgcctcga catcagcaag taccagcgct tccatgagtg
     2041 cgtcgacgtc agccagcacc agtgcttcaa caagtgcaag tatgagtgct tcaacaagtg
     2101 caagtaccag cgcctcaacc agtgccagta ccagtgcttc cacctcagca agtatgagcg
     2161 cctcaacaag tgcaagtact agcgcttcca caagtgccag taccagtgca tccacgtcag
     2221 caagtactag cgcctcaatg agtgcctcga cgtcagccag caccagtgct tccacaagtg
     2281 caagtactag cgcctcaatg agtgcctcga cgtcagccag caccagtgct tccacaagtg
     2341 caagtactag cgcctcaaca tcggcaagta caagttcttc cacaagtgcc agtaccagtg
     2401 catcgacatc agccagcact agcgcctcaa tgagtgcctc gacgtcagcc agcacaagtg
     2461 cttcaatgag tgcctcgacg tcagccagca ctagcgcctc aacgagtgcg tcaatgtcag
     2521 ccagcacaag ttcttcaaca agtgcctcga tgtcagccag cactagcgct tcaatgagtg
     2581 cctcgacgtc agctagcact agcgcctcaa cgagtgcgtc aatgtcagcc agcacaagtt
     2641 cttcaacaag tgcctcgatg tcagccagca ctagcgcttc aatgagtgcg tcgacgtcag
     2701 ccagcaccag cgcttcaacg agtgcgtcaa tgtcagccag caccagtgct tcaacaagtg
     2761 cctcgatgtc agccagcaca agtgcttcaa caagtgcaag tatgagtgct tccacaagtg
     2821 caagtaccag cgcctcaacc agtgccagta ccagtgcatc cacgtcagca agtatgagcg
     2881 cctccacaag tgaaagtatc agtgcatcca cgtcagcaag tatgagcgcc tcaatgagtg
     2941 cctcgacgtc agccagcacc agtgcttcca caagtgcaag tactagcgcc tcaatgagtg
     3001 catcgacatc agcaagtacc agcgcttcaa cgagtgcgtc aatgtcagcc agcaccagtg
     3061 cttcaacaag tgcctcgatg tcagccagca ccagtgcttc aacaagtgcc tcgatgtcag
     3121 ccagcactag cgcttcaatg agtgccacga cgtcagccag caccagcgtc tcaacgagtg
     3181 catcgacatc agcaagtacc agcgcttcca caagttcttc aagctcagtg acttctaatt
     3241 catcaaaaga gaaggtgtat tctgccttac cttctacggg tgaccaagat tattctgtaa
     3301 ctgctactgc cttaggttta ggtttaatga ctggtgcaac ccttttggga cgaaaaaaat
     3361 ctaaaaaaga taaagactaa acctactttg aattcttaaa gtactaaatg acatggtaaa
     3421 aggcatatct cttaatattt ttagagagat atgccttttt gttgtagaag tttgtaaaaa
     3481 gccttgtaaa tctatattta tcaagtgaca agcaatgttt ttctgctaaa atatgataaa
     3541 attaagaagt ggaatttatt tctgaaaata agtttatcaa ttacgataaa ctcaaaaata
     3601 atgtaaaaaa gctttggatc attttagtac aatgagtgtt aataaaacta ttatttaact
     3661 ggtatgatat ggtctggata agagagagtt ttaaagagag attaactaaa atgcgagtac
     3721 atattacaag tatatatggg cagtcaccgc gtagcattgc ccttatatca caaaaactag
     3781 ttaaggatgt cggacgtcaa cttggttatg atgaaatggg aatctacttt tataatgatc
     3841 atgctgaaac gcatggtgaa agaagtacta ggatggatgg tatcattgcg ggattaggca
     3901 gaggggatat tgttgttttt caagtaccta catggaattc aacagaattt gatgaacttt
     3961 ttctagataa attacaggca tacggtgcta gaattataac ttttgtacat gatattgttc
     4021 ccttaatgtt tgagtctaat ttttatttat tagatagagt aattgacatg tataatcgtt
     4081 cggatgttgt tattctacct acaaaagcga tgcatgatta tctaattgaa aaagggatga
     4141 ctacctctaa agttctttat caagaagtat gggatcatcc agttaatata gacctccctc
     4201 gtccagaatg tcaaaaagtc cttagttttg caggcgatat tcaacgcttt ccctttgtca
     4261 atgactggaa ggaaaatatc ccgctcatct attatggaga cggaagcaga ctaaattctg
     4321 aggcaaatgt tcatgctcag ggatggaaag atgatgtaga attgatgcta agtctttcta
     4381 agcggggagg atttggcctg tgttggagtg aagatagaga ggagttagtc gagcgtcgtt
     4441 attcgaggat gaatgcctcg tataaattat ctactttctt ggctgctgga cttccaatta
     4501 ttgctaacca tgatatttca agtagagact ttatcaaaca acacggatta ggatttacag
     4561 ttgaaacgct agaagaagct gttgaaaaaa ttaataatat ggaaaaagag acttatgaca
     4621 gctatgtgga aaatgttgag aagatagcaa ctctcttgcg aaatggttat atcactaaaa
     4681 aattgttgat agatgctgtg catatgcttt atagataggg gtagcgatga agaaaacaat
     4741 tgttttaggt gcagatttcc aatatcgtga tcaagttatg acaactataa aatctattgt
     4801 tagtcataat caacatttaa ctatttatat cattaacaca gattttcccg tagaatggtt
     4861 taatatcctt aaccattctt tagaacagtt tgactgtaga gtaaaaaata ttcccataag
     4921 ttcagatgtc tttgagggga tacctaccct gagccatatc agtgtggcgg gatttttcag
     4981 atggtttatt ccaattcacc ttgaagaaga gatagtttta tatttagata gtgatgtaat
     5041 agttaggggt tctttggatc ctctatttga cataaacctt gaagagaatt tattaggagc
     5101 tgtagcagat catttttcaa cgctttacta tggagataca gcaccagtta gttttaattc
     5161 aggtgttatg ctaatcaata attcattatg gaaaaaagaa gaaatttata atagccttat
     5221 gaggattgct gataagggtt ctgctgttgg ggtaggagat caagaatacc ttaatatttt
     5281 gactcagaat cgttggatag atatcggtaa acaatataat gttcagattg gtcaagatgt
     5341 caatattaat gcatatgggc gaccagattt gtatcatttt tatgatgatt gcgaacctgt
     5401 tattgttcat tataatagtc aagataaacc gtggaataaa tatagtcaat cgcgataccg
     5461 ttcagaatgg tggtattatt ttggtttaga atggtcagtt atttatgctc aacaacaaaa
     5521 aaatttgaac aggttaacag gtaaaacact gaatttattt acattaacag cctcagatac
     5581 attagaacat attgaagtac ttgcaaaagc tctaccagat tatcagtttc atattgcagc
     5641 acgtagtgat atttcagaga aattagctaa tctagaaact tatagcaata ttaaagttta
     5701 taaattagtt atcaacccta tcttggaaga gcttatacgt aactgtgatg tgtacttgga
     5761 tattaatcat tcttacgaag tagaaggtat tctagataaa ataaaagaag agaataaacc
     5821 gattttagcc tttgattcta cagcacatac gacaaaaaat atagaagtta tctcagaaac
     5881 aatgcctgaa ttgatggtta aaagaataag ggaagtaggt cctcttagat gaaaaataga
     5941 aaagctatcg cgttagcggc agattttggc tatcaagagc aagttaagac tataattaaa
     6001 tcaatatgct ttcataatca atttattgat ttttatatct taaatgacga ttttccagtt
     6061 gaatggtttc aaatgatgga atatcattta tctaaaatgg attgcacaat atctaacact
     6121 aaaattttta atgaagaaat taaacatttt aaatttcaaa aaccgatgcc ctatcctact
     6181 tactttcgat actttatacc tgaagttatt catgaagata aggtactata tttagactgt
     6241 gatatgatta tcaccagtga tttaacatcg atatttactc ttgatatttc aaaatatggt
     6301 gtagcggctg ttcgagatga tttgctcgaa gaatatgatg gtaaagaaga ttattttaat
     6361 tcaggtttgc tattaattaa taatattttt tggcgagaac aagggattag tcagcgactg
     6421 ttagattata ctagagaaaa ccaaggggct cttcaatatc atgaccagga tgttttgaat
     6481 gatgtactgt gcgataattg gctagaactt gatgaaactt ataactatca tacaggagca
     6541 gatatgttat ataacctatt ccaacagtct gaacgtcaat taaatcgcag gaaagattta
     6601 cctaaagtta ttcattatac agcgacaaaa ccatggaaat accttgaaac atctgttcgt
     6661 tggcgcgata tatggtggga atataataga cttgaatggc gcgatatttt tacaagatgg
     6721 caagtcaatg atggtgtaga tgtttcattg aagaaagtgt tacaacctat ccatcgagct
     6781 ttcattttta cttactcaga ttcaattgaa ggaattgaga ggttggctaa ggatttacca
     6841 aactggcaat ttcatattgc tgcctatact caaatttcag aaaaattgaa acaattggaa
     6901 atgcattcaa atataaaact ttatccactt gtttatcgtt tcaaattaaa tgagctagtt
     6961 gaaaattcag atatttacct tgatattaat catggtgatg cagatactca ggttctggag
     7021 caagtgaaga aattaggtaa agatatttta tccctttcta caacttatca tggtcaaggc
     7081 aaacaatttg agagagttac ggatattatt ga
//
LOCUS       DATRSD010000085         7005 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRSD010000085 DATRSD010000000
VERSION     DATRSD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.086"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..208)
                     /locus_tag="VBD85_001684"
     CDS             complement(<1..208)
                     /locus_tag="VBD85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134225.1"
                     /translation="MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFSFIFL
                     IYQYDRETIFKTIAIVIFFLFYFLW"
     gene            complement(192..845)
                     /locus_tag="VBD85_001685"
     CDS             complement(192..845)
                     /locus_tag="VBD85_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO7134226.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID"
     gene            complement(945..1685)
                     /locus_tag="VBD85_001686"
     CDS             complement(945..1685)
                     /locus_tag="VBD85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO7134227.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            1820..2584
                     /locus_tag="VBD85_001687"
     CDS             1820..2584
                     /locus_tag="VBD85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO7134228.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTNATIIEIELQESLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            2577..2843
                     /locus_tag="VBD85_001688"
     CDS             2577..2843
                     /locus_tag="VBD85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7134229.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRQAKLTYIKQHKNEQ"
     gene            complement(3028..4614)
                     /locus_tag="VBD85_001689"
     CDS             complement(3028..4614)
                     /locus_tag="VBD85_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7134230.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPETRQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRCDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(4849..5679)
                     /locus_tag="VBD85_001690"
     CDS             complement(4849..5679)
                     /locus_tag="VBD85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134231.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKKIKDKNSKEKVITVAT
                     YSKPTSTFLDLVKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGANVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEAAVAF
                     "
     gene            complement(5718..6356)
                     /locus_tag="VBD85_001691"
     CDS             complement(5718..6356)
                     /locus_tag="VBD85_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134232.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVIILQSLGYL"
     gene            complement(6349..>7005)
                     /locus_tag="VBD85_001692"
     CDS             complement(6349..>7005)
                     /locus_tag="VBD85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7134233.1"
                     /translation="EEGEIVGLIGSNGTGKSTILKMINGLIPYDKGNIYYQGKEVKSF
                     SDNKLRQMRKDIAYIFQNHNLLAGESVYYHLALVYKLNHQKVNHDAINDILDFLGLMD
                     LKQVKCHSLSGGQQQKVAIAMAVLQKPKLILCDEISSALDTNSEKEIFNLLSDLREKY
                     GISILMIAHHLSLLKQYCDRVMILDHQTIVDTVVPVKATLNQLKSNYVDQVKEFLLHD
                     "
BASE COUNT         2311 a         1322 c         1061 g         2311 t
ORIGIN      
        1 gccataaaaa ataaaataag aaaaaaatta ctattgctat tgttttaaaa atagtttcac
       61 gatcatattg ataaatcaga aaaataaaag aaaataaaag aaaggcacaa ccaagcattg
      121 tccaagaaaa cactagtaag taaatttgga agaccaacaa tgctaaataa ataggtttta
      181 gaggaaaata cttagtcaat tgtaacatct tgacgtaatt tttctagtgt tttctcacca
      241 atacctgata cgtttttgag gtcatcaata gttttaaaac caccttgact atcacgataa
      301 tctaaaatat cttgagctct tttagcccca acgcctgata tggtctgaag acttgataaa
      361 tctgctttat taagattaat tttcccctta ctatttccct gagtagcgat attatttgct
      421 ttctcctctt ccacaacagt tattttttca cctttttgag caacatagat aaccgtctca
      481 tcttgtaatt tctgtgctag attaacggat ttactatcag cgagattgct tagtccacct
      541 gcacgcttaa tcgcatctgt tacacgactt tgagaaggca aactataaac acctggatga
      601 ttgactgccc cttttacgtc aaccgttact tgattagata ctttatcttt gacgtgacta
      661 atcattggta cctttttttc tgttatagtt gatacttttt tctctaaatt gatatttaaa
      721 ttgtcttctt tatgatgagt gccaaaatga tttaaaccca aaattccaaa taataagagt
      781 cctacaataa ttcctgttgt ctcccattta tgcgatttaa ttttttccaa tactatttca
      841 aacataaaaa tattcctcca tttacttatt cgtaactaaa gagattttcc tattaaaaac
      901 caaaaaaact atttactaaa aagtaaatag tttccatgta acccttattt tagatgtttt
      961 tgtggatccc acacaaagct tgctaaataa ctaaaaatta gagttaggac aagcaagaca
     1021 atagctataa ttgctaaagg tagacgataa atataagtca atggatttaa tttcttacca
     1081 tcatcataag tttcattttc tctatctaaa cgatcaaatt cttcctgaat acgacgagat
     1141 acttctgcaa tcccttcggc atccatacgc ttaatatcag aaacatcaat agggttacca
     1201 aaattcatat caacacgatg cccctttaat agatttttaa aaaccatagg acctctatat
     1261 gctgctggca taattctgac ttttgccatt tttgcaataa cagctacgcc tcctttgaca
     1321 tctttggagt ggcggcttcc actcggaaac atcaccaaag aacggttgct atttttcagc
     1381 atctttacag gatatcgaat agcatcttgc cctggctttt cacgatcaat agggaaagca
     1441 ccacacattt taatccacca accaaacaaa cgattcgtga ataattcttt tttggccata
     1501 aaaataaact gtttgggtct agcagcaaaa gccatgtaca caggatccca aaatgttctg
     1561 tgtggagcta ctaaaatata attttcatca tcttttaaca ttttatcttc attatgatag
     1621 tgcgcattac cattagccac ccaaatcaag aacataacta aagtacgtaa gtaagtatag
     1681 aacatgtttc tcctctacct ttcatcaagt aacttctatt ttatcatgct tccttttttt
     1741 tagaaagaat tatcttaaaa tcaacctcca cactttcaaa actagtgcta ggaagctgta
     1801 attttgctat aataagccta tgatagatac aattttaaaa gaaaatgaac gcattgatca
     1861 gcttttttca actgatgtca aaatcatcca aaacaaagaa gttttttcct attccataga
     1921 tagtgtttta ttgtctcggt tccctaaact tccttctcgt ggtcttatcg ttgacttatg
     1981 tagtggtaat ggtgctgtcg gtttgtttgc cagcaccaaa acaaatgcga caattataga
     2041 gatcgaacta caagagagcc ttgcggatat ggctaagcgt tcgattaagc tcaataaatt
     2101 ggaaaaacag gttactatga ttaatgatga tttaaaaaat cttcttgatc atgttcaacg
     2161 ttcaaacgtt gatttaatgc tttgcaatcc accttatttc aaagcaagcg aaacctctaa
     2221 aaaaaatcta agcccgcatt atctccttgc aagacacgaa ataacaacaa accttagaga
     2281 gatttgtcaa attgctcaac atgctctaaa aacaaaagga cgtatcgcta tggtacatcg
     2341 tccagaccgt ttccttgaaa ttattgatac gatgcgtcag ttcaatttag cacctaaacg
     2401 tatccaattt gtttatccaa aattaggaaa agatgctaat atgcttttaa ttgaagctat
     2461 taaagatggc tcgacagaag gtatgaaaat acttcctcct cttgttgttc atcaagataa
     2521 tggagactat actgagacta tttttgatat atatttcgga gaaaacggaa aatctcatga
     2581 ttaaagttcc tgcttatatg tatgttttag aatgttcaga tggaacactt tatacaggtt
     2641 atactacaga tgtcaagcgc cgtctcaata cacacaatac tggaaaaggt gctaaatata
     2701 ctcgtgcgag attgccagta aaacttcttt attctgaagc ttttaatagc aaacaagaag
     2761 caatgagggc agaagcatta tttaaacaaa agactcggca agcaaaattg acttatatta
     2821 aacaacataa aaatgaacaa taatacgata atatactaag tgtaatgagc ctatcctcgc
     2881 ttctttaact tttaaatttc aacctcactt taatcttttg tcttcattct taaagttata
     2941 aggcaatttt atggatatca ctatctacgc tttatcttac aaaaaataaa aaaggagtcg
     3001 ctcagcaact cctttttcat aatcctctta tttatcgcct ttgttgcgaa taacaaatcc
     3061 agtttgtttt tcacttgaag tgtttttatg aggtttttta ttttcttgac gacgattatc
     3121 tttcttgaac ttatcatcac gacgtttaaa atcacaacga ccaccacgat catcacggtc
     3181 acgatttcca cgacgatcac cacgatcatt gcgtcgacga tcgccaccac ggccattgcc
     3241 tcgtccacct ttacctttaa agccaccacc tgatggttta aatggtaatg gtttttcacg
     3301 tgtgatttca acttctggta acgattctgg atcctgaact gttaagctta agacataaag
     3361 agcaagttct tctggtgtgt attgagttgc taattctaaa gcatcggctt taaatttatc
     3421 aaatttagaa actaattcct gatcctcaaa gtcacgtgca atacgtttca atgcaacttt
     3481 tttcttagcc tgaaatgctt cgcttgcagt agcaggcttc attccagtca tacgtttttt
     3541 agttaagttt tcaataattg tcaaatatcc catttcgttt ggtgaaacaa aagtgataga
     3601 ttgaccagat ttaccagcac gaccagtacg tccaatacgg tgaacataac tttcgggatc
     3661 ttggggaata tcatagttgt aaacatgtgt aacgcctgaa atatctaaac cacgcgcagc
     3721 aacatctgtg gcaacaagaa tatcaatatg gtcattttta aagtctctga taacacgaag
     3781 acgtttattt tgatctaagt ctccatgaat tccttctgct cggaaaccac gtaattttag
     3841 tccacgagtt aattcatcta cacgacgttt cgtacgtcca aagacaattg acaattctgg
     3901 ttgatcaacg tccatcaaac gtgtcatagt atcgaatttt tcattttcct taacacgaac
     3961 ataatactga tcaacattca cattagttaa ttcagttgct ttaattttaa catgctcagg
     4021 atctttcatg aatttaacgc cgatacgttt gattggatca ggcattgtcg ctgagaataa
     4081 aagagtttga cgtgtttctg gtacacgact aatgattgct tcaatgtcct ctaaaaatcc
     4141 catgttaagc atttcgtctg cttcatcaag aataagagtt tcaatgtgat taagtttaag
     4201 tgctttacgt ttaattaaat caagtaatcg acctggagtt ccaacaacaa cgtgtgctcc
     4261 tgaacgtaat gccttgattt gtttttcaat acttgaccca ccataaacag agcggacctt
     4321 aacaccttta tcgcgtccaa aacggaacaa ttcttcttgt gattgtactg caagttcacg
     4381 tgtaggagca ataataagtg cttggatagt attatcttca gtatgaattt tatttaaagt
     4441 tggtaaccca aaagctgcag tttttcctgt acctgtctgc gcttgaccaa tgacatcttt
     4501 gccttcaaga gcaagtggga tagtcatttc ttggattggt gatggctcta caaagcctgc
     4561 tttttctacc gctgaaagaa tatcttgcga taagttaagt tctgtaaatt tcaaatgttt
     4621 ctcttttcta aaagacagtg cgaagctgcc ttatgaacct ttattagtta tcttttctga
     4681 caacctactt agtgtaacac aattaaaagt aaaaagatat aaaataaggt caatctaata
     4741 aataacacgc tttcataaat tgctagttct attagttgct tcagaaaata gaaaaaaggc
     4801 aataaaaacc caccatctat aaagaaggag ggttcatagt ttatctatct agaacgcaac
     4861 ggctgcttca cttggaatct ccttagtgat atagtgacga atctctttac ttgttagtac
     4921 ttctttaagt acttttattt tatcactatt tttattatct tcacgagcta ccaaggcacc
     4981 tgcaaaacgg atatctggtt ttttctcata taataatcta tcccttttag gaactagatt
     5041 gatttttgtc acatatccag ggaaattaaa gacaaggtca tattcaaaat aggcctgatt
     5101 gaggttaagt aatgctacag gttcaattcg taatttttta gggttagtaa taatatcttc
     5161 gatagccttg gtctttttgg acgtattctt taaggtgata agtttctttt cttgcaataa
     5221 tagcagagct ctagtcatat tggcaggatc agacggaatc gctacattag caccgtcttt
     5281 aagctcggca atatttttca aatgttgacc ataaaaacca gccaatgaat gataaattgg
     5341 tgtaattgat actagatgac catcattttc cttattaaag atactcatga aaaattcatg
     5401 ttgtaaaagg ttagcatcat gttctttgtt ttctaaagca atgttagcct gaatatagtc
     5461 agagaccatg acaaccttta aagtatatcc tttttctttt acattatctt taaccaaatc
     5521 taaaaaggta gatgtaggtt tgctgtaagt tgcaacagta atcacctttt ctttactatt
     5581 cttatcttta attttcttag acgacattgc ttgcttagta catgccacta aacataaaga
     5641 catcatgatt agacatgtta gggaaagtaa ttttttcata gttcctcttt tcttttattt
     5701 tcttgagtag cggtttgcta taaatagccc aaactctgta agataatcac atatattata
     5761 aataagacaa caatcaagta cataactggg tagtcatact cttgatagcc aaaacgatag
     5821 gcatattcac ccaaacctcc tgcaccaata actcccatta ttgtcgagta gccaagaata
     5881 cttatagccg ttgcagtaaa tgataaaaca aggtcaatct tgatggaggg gattaaaaaa
     5941 taataaatta tctgtctctt gttagcccct agactgagag ctcgatctac aacaacttga
     6001 ggtatattta ataaagcttg ttccacataa cgtgcataca aactgactga aaccaaagta
     6061 agaggtaaga ttgccgctat cgttccaaat gaggtcccaa aaattaatcg attgagaggg
     6121 attaagataa aaataaaaat aaggaaaggg acactccgta acgtacctaa aaataaattt
     6181 agtagttgat aggctaaaga atgtttaatc aagtaagact ttctcaatga aaacaataaa
     6241 atacctgttg gaaaagcaat tagaaaacaa agaatcaagg ttaaccctaa cattaaattg
     6301 gtttcccaaa atgattttaa gataccttcg ccatgttttt caataatgtc aatcatgtaa
     6361 caaaaactcc tttacttgat ctacgtagtt tgactttaat tgattcagag ttgctttcac
     6421 tggaacaaca gtatctacaa tagtctggtg atccaaaatc attacacgat cacaatattg
     6481 ttttaaaaga gataggtgat gagcaatcat caagatagag ataccatatt tttcacgaag
     6541 atcagacaac aagttaaata tctctttttc actattagta tctaaggcag agctgatctc
     6601 atcacataga ataagtttcg gtttttgcaa aacagccata gcgattgcaa ctttttgttg
     6661 ctgcccacca ctcaaactat ggcatttcac ctgttttaaa tccatcaaac ctaaaaaatc
     6721 taagatatca ttaattgcgt catgatttac tttttgatga tttaatttat aaactaaagc
     6781 tagatgataa tatacacttt ctcctgctaa caaattatga ttttgaaaaa tataagcaat
     6841 atctttacgc atctgacgta acttattatc agaaaaagac ttcacttctt ttccttgata
     6901 ataaatattt cctttatcat aagggattaa gccattaatc atcttcaaaa tagtactctt
     6961 tccagtacca ttagaaccaa tcagaccaac tatctcgcct tcttc
//
LOCUS       DATRSD010000086         6862 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRSD010000086 DATRSD010000000
VERSION     DATRSD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.087"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..923)
                     /locus_tag="VBD85_001693"
     CDS             complement(<1..923)
                     /locus_tag="VBD85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEO7134234.1"
                     /translation="MVNNRKIETLGLSYLRTVLDKNNYLQTYFDENDKTPLWDGEIHL
                     LRAPSEKKSDIIGKVPVQIKATQQKKKNNRFLISLSDLELYQKNGGFVLFVVWIDEEN
                     NIKEILYKSLPPLSIKNLIKRSKRKLTTGAPKKVSIEIHSLSENKVYPMLMDFINSSR
                     KQYSFATSRGLTMDKLPKEPKLKFYHYGKSPIDVFDYQEEHELFAYMVDDDTGIEIPI
                     ENPIKIVETAEKTDLTFTIGNYTIKNVVRCRFPNGNVELQIGSGFKLQADEKAGKFRL
                     NYSRPDLLNQSIECTKALQELQNVGYVKLNEA"
     gene            complement(1061..3046)
                     /gene="tkt"
                     /locus_tag="VBD85_001694"
     CDS             complement(1061..3046)
                     /gene="tkt"
                     /locus_tag="VBD85_001694"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7134235.1"
                     /translation="MTFDAVDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NKYMNINPKTSRNWTNRDRFILSAGHGSAMLYSLLHLAGYDLSIDDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKYNKPGFNIVDHYTFALNGDG
                     DLMEGVSQEVASLAGHLKLGKLITLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLDAIAKAIEEAKAETEKPTIIEVKTIIVFGSEKQGTSAVHGAPLGAEGIVFT
                     KNAYKWTYEDFIVPDEVKARFQEGLQARGEQAENAWNELFAKYEAEYPELAKGYKEVF
                     SNEPVSVELAAHELGSSKASRVSSQEAIQQISEQVASFWGGSADLSASNNTMVKVEKD
                     FQPGQYEGCNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQS
                     LPTMYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKHALAEI
                     DKPTMLVLTRQNLPVLEGTKELAEEGLNKGAYILSEAKGEMDGIIIATGSEVKLAMDT
                     QAALAEEGIHVRVVSMPSQNIFDAQSDAYKEEILPATITKRLAIEAASSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTTENATVLYKAL"
     gene            complement(3095..3739)
                     /gene="fsa"
                     /locus_tag="VBD85_001695"
     CDS             complement(3095..3739)
                     /gene="fsa"
                     /locus_tag="VBD85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7134236.1"
                     /translation="MKFFLDTANVEAIKTINELGLVDGVTTNPTIISREGRDFETVIK
                     EICDIIDGPVSAEVTGLTANEMVDEARALSKWHDNVIVKIPMTAEGLKATSILSKEGI
                     KINVTLIFTVSQGLMAMKAGASYISPFIGRLEDIGTDPYQLIEALRDIIDIYGFGTEI
                     ISASIRSAAHVEAVAKLGSHIATIPETIFDKMVQHPLTKSGIDQFMNDWASFKK"
     gene            complement(3757..4446)
                     /locus_tag="VBD85_001696"
     CDS             complement(3757..4446)
                     /locus_tag="VBD85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7134237.1"
                     /translation="MQKVEISPSLMCMNLDKFQEEICFLDSHVDSYHIDIMDGHFVPN
                     ITLSPWFVEEVRKLSSLPMSAHLMVKDQPFWVQQMIDIKCEWICMHAEVINGLAFRLI
                     DQIHNEGLKAGVVLNPETPIEVIMPYIELLDKVTIMTVDPGFAGQRFLDSTLDKIVDL
                     RRLREENNYNYLIEMDGSSSRKTFKKIDEAGPDIYIVGRSGLFALTDNISESWDIMTN
                     DYFEMTGKKLN"
     gene            complement(4458..5561)
                     /locus_tag="VBD85_001697"
     CDS             complement(4458..5561)
                     /locus_tag="VBD85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEO7134238.1"
                     /translation="MKDMIKKANFKGHLLTAISYLIPIVCGAGFLVAIGMAMGGRAQD
                     ALVIGNFSFWDTMATMGGKALGLLPVIIATGIAFSIAGKPGIAPGFVVGLIANAISAG
                     FIGGILGGYIAGFIALGILKNVKVPNWAKGLMPTLIVPFFAALLSSLIMIYLIGGPIS
                     AFTGWLTDFLKSLGNTSNLVMGAVIGVLSGVDLGGPLNKTVYAFVLTLQAEGVKEPLT
                     ALQLVNTATPVGFGLAYFIAKLFKKNIYTNDEIETLKSAVPMGIVNIVEGVIPIVMNN
                     LMPAILAIGVGGAVGGAVSMTMGADSAVPFGGILMIPTMTRPIAGICGLLSNILVTGL
                     VYSLAKKPVDRNEVTIVSAEDEEDIALDDIEIM"
     gene            complement(5574..6023)
                     /locus_tag="VBD85_001698"
     CDS             complement(5574..6023)
                     /locus_tag="VBD85_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7134239.1"
                     /translation="MEARDAIKRKLIKTNMNVTTKDEAFSELVSLLKNEGFVSDEGGF
                     IKDIYLRESEGQTGIGNYIAIPHGKSSHVTEPAVVIGINSSEIPWETLDDNGVKVIVL
                     FAVGNDSIAAQEHLKILSLFARKLGNDQIVEKLINAKNEDDVIEAFS"
     gene            complement(6037..6348)
                     /locus_tag="VBD85_001699"
     CDS             complement(6037..6348)
                     /locus_tag="VBD85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEO7134240.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENASKAAGHDVKIETQGTIGI
                     ENQLTSEEIAAADIVLLAVDVKIAGEERFEGKKVVKVPTEVAIKSPNKLIAKLVEVAS
                     N"
     gene            complement(6362..>6862)
                     /locus_tag="VBD85_001700"
     CDS             complement(6362..>6862)
                     /locus_tag="VBD85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7134241.1"
                     /translation="KNEEITQLLTGNPYSNNVTIGKKDQIEANYYIPMLNQDFKTKEE
                     LLNSVGQNLVEMGYVQKEFVASLLDREKMGSTEFPLGVALPHGRPEFVLKTVVVFIQN
                     THKIKWKESLVDTVFLIALSRDDIKNVKDIILKINKTISNEEVLRQLKSETRELEVYK
                     LLNQAD"
BASE COUNT         2034 a         1400 c         1060 g         2368 t
ORIGIN      
        1 gcttcattta atttaacata acccacattt tgtaattctt gaagggcttt agtgcattct
       61 attgattgat ttaataaatc tggtctcgaa taatttaatc taaattttcc tgctttttca
      121 tcggcttgca atttaaaacc tgaaccaatc tgtaattcta cattaccgtt tggaaaacga
      181 catcgaacca cattttttat agtataattt ccaatagtaa aggtaagatc tgtcttttca
      241 gcagtttcaa caatttttat tggattctca attgggattt caatacctgt atcatcatct
      301 accatataag caaaaagttc atgttcttct tgataatcaa aaacatcaat tggacttttt
      361 ccgtaatgat aaaatttcaa ttttggctct ttcggcagtt tgtccatagt caatccgcga
      421 ctagtagcaa aactatactg cttgcgactt gaattgataa aatccatcag cattgggtaa
      481 accttatttt ctgataagga atggatttca attgacactt ttttaggtgc ccctgtagtc
      541 aattttcttt tacttctctt tataagattt tttattgaaa gaggtggaag agatttgtat
      601 aatatctctt ttatattatt ctcttcatca atccatacta caaacagtac aaatccacca
      661 tttttttgat acaattctaa atcactcaga cttatcaaaa atctattatt ctttttcttt
      721 tgttgagttg ctttaatttg aacaggaact tttcctataa tatcagactt tttctcgcta
      781 ggtgctctta gaaggtgaat ctctccatcc caaagtggtg ttttatcatt ctcatcaaag
      841 taagtttgta agtagttgtt cttatcaaga acagtacgta aataagacaa tcctaacgtt
      901 tctatttttc tattgtttac caaatcacac ctcgataagc ttctgactac attatatcat
      961 gtatctaaat aatatataga atttctcaga ttcttatttc ataacttttt aaacagaaaa
     1021 aaacaagatg atattgaatc atcttgcttt ttgattattt ttataatgct ttatatagga
     1081 ctgtagcatt ttctgttgtg aaaccatatt cttcaaagat acgatttcct ggtgccgaag
     1141 ctccccatgt gtcaatggtt aaggttttgc cagctaagcc tacatatttt gcccaaccaa
     1201 agcttgatgc tgcttcaatt gccaagcgtt ttgtgattgt ggctggtaaa atctcttctt
     1261 tataagcgtc gctttgcgca tcaaagatat tttgtgatgg cattgataca acacgaacat
     1321 ggatcccttc ttctgctagc gcagcctgtg tatccatggc taattttact tcagatcccg
     1381 tcgcaatgat gataccatcc atttcgcctt tggcttctga caagatataa gcacccttat
     1441 ttaaaccttc ttctgctaat tcttttgttc cttcaagaac tggcaagttt tgtcgtgtta
     1501 acactagcat tgttggttta tctatttctg ctaaggcatg tttccatgcg gcattggttt
     1561 cattaccatc cgctggacga atgacgttca agtttggcat tgaacggaca cttgctaact
     1621 gttcaattgg ttcatgagtt ggaccgtctt caccaacggc aatggagtca tgtgtcatga
     1681 catacatggt tggtaagctt tgcagtgctg ccatacgtac tgcaggtaat agatagtttg
     1741 agaagacgaa gaatgtacca ccataaacgc gagtaccacc atgaagagca ataccgttca
     1801 tcgcagctgc catggcaaat tcacgtacac caaaccagat attgcagcct tcatattgac
     1861 ctggttggaa gtctttttca actttaacca ttgtgttgtt tgaggctgaa aggtctgccg
     1921 aaccacccca gaatgatgcg acttgttcag agatttgttg gatagcctct tgacttgaaa
     1981 cacggctagc ttttgatgac ccaagttcat gcgcagctaa ttctactgaa actggttcat
     2041 ttgaaaagac ttctttatag cctttagcaa gttctggata ttccgcttca tattttgcaa
     2101 agagttcatt ccaagcattt tcagcttgtt caccacgtgc ttgtaatcct tcttggaaac
     2161 gagctttcac ttcgtcagga acgatgaaat cttcataggt ccatttgtaa gcatttttag
     2221 taaagacaat gccttctgct ccaagtgggg caccatgaac tgctgaagtt ccttgttttt
     2281 cagatccaaa aacaataatt gttttaactt caataatagt tggtttttca gtttcagctt
     2341 ttgcctcttc gatagctttg gcaatagcat ctaagtcatt gccgtcttta acgaggatat
     2401 gttgccatcc ataagcttca aaacgtcctt taacatcctc agtgaaggcc atagatgtag
     2461 ggccatctag agaaatgtca tttgaatcat ataaagtgat taattttcca agttttaagt
     2521 gacctgctaa acttgcgact tcttgactga caccttccat taagtcacca tcaccattta
     2581 aagcaaaagt ataatggtca acgatattga aacctggttt gttgtatttt gcagcaaggt
     2641 gagcttctgc cattgccata cctacagcgt ttgcgatacc ttgtcctagc ggtcctgttg
     2701 ttgcttctac accatctgta tgattgactt caggatgtcc tggcgttttt gaaccccatt
     2761 gacggaaatt tttcaaatca tcgattgaaa ggtcgtaacc agctaaatgt aataagctgt
     2821 atagcattgc agatccatgt cctgcagaaa gaataaaacg gtctctgttt gtccagttac
     2881 ggcttgtttt aggattaatg ttcatgtatt tattccatag aacataagcc attggagctg
     2941 cccccattgg taaaccgggg tggcctgaat tagctgcttg gatagcatcc attgaaagcg
     3001 tacggacagt gtttactgcc aactggtcaa ctgcatcgaa tgtcataagt aaaacctttc
     3061 tgtttttagt aatataaaaa ggggattccc ctttttattt tttaaatgaa gcccaatcat
     3121 tcataaattg atcaatacca ctttttgtta aggggtgttg taccatcttg tcaaaaatgg
     3181 tttccggaat tgttgcgata tgtgatccca atttagcaac tgcttcaaca tgagctgcac
     3241 tgcggatgct agcagaaata atttctgtgc caaaaccata tatgtcaata atgtcgcgaa
     3301 gagcctcaat taattgataa gggtctgtac cgatatcttc taaacgccca ataaatggac
     3361 tgatatatga tgcgccagct ttcatggcca taagaccttg tgaaaccgta aagataaggg
     3421 tgacattgat tttaatgcct tcttttgaca aaatactggt tgctttcaat ccttctgcag
     3481 tcattggaat tttaacaata acattgtcat gccatttaga tagtgcacgc gcttcgtcca
     3541 ccatttcatt agctgttaat cccgttactt ctgctgaaac tggaccatca atgatgtcac
     3601 aaatttcttt aataaccgtt tcaaaatcac gaccttcgcg agagataatc gttggatttg
     3661 tcgttacacc atccaccaaa ccaagttcat tgatagtttt aatagcttca acattagctg
     3721 tgtctaaaaa aaatttcatt tctatcctct atttatctag tttaattttt ttccagtcat
     3781 ttcaaagtag tcattggtca taatgtccca tgattctgaa atattatctg ttaatgcgaa
     3841 taaaccactt cgtccaacaa tgtatatatc agggccagcc tcatcaattt ttttaaatgt
     3901 tttacggcta gatgaaccat ccatctcaat taagtaatta taattatttt cttctctcaa
     3961 tcttcttaga tctacaattt tatctaaagt tgaatctaaa aacctttgtc cagcaaaacc
     4021 aggatccact gtcataatag taactttatc taacagttca atatagggca ttattacttc
     4081 aataggggtt tcgggattta aaactacccc tgcttttaaa ccttcattat gaatttgatc
     4141 aattaaccta aacgccagac catttatcac ttcagcatgc atacaaatcc attcacattt
     4201 tatatcaatc atttgttgca cccaaaacgg ttgatctttt accatcaaat gagctgacat
     4261 tggtaaggag gatagctttc taacttcttc cacaaaccaa ggagacagtg taatattagg
     4321 tacaaaatgt ccatccatta tatcaatatg gtaggaatca acatgtgaat ctaaaaaaca
     4381 gatttcttct tggaatttgt caagattcat acacatcaac gagggagata tttctacttt
     4441 ttgcatcata gttctcctta catgatctcg atatcatcaa gtgcgatatc ttcttcgtct
     4501 tcagcagaaa caattgtaac ttcattacga tcaactggct ttttagccaa tgaatatact
     4561 aatcctgtta ccaagatatt agataacaag ccacaaattc cggcaattgg cctagtcatt
     4621 gtaggaatca ttaaaattcc accgaatggc actgcagaat cagctcccat tgtcattgaa
     4681 acagctcctc ctacagctcc accgacacca attgctaaaa ttgcaggcat taagttgttc
     4741 attacaatag ggattacccc ttcaacaata ttcacaatcc ccattggcac tgcagatttt
     4801 aaggtttcaa tctcatcgtt agtataaatg tttttcttga ataatttagc aataaaatag
     4861 gctaaaccaa atcctactgg tgtcgctgta ttaactaatt gtaaagcagt aagtggttct
     4921 tttactcctt cagcttgtaa ggtcaataca aatgcataaa cagttttatt caatggtcct
     4981 ccaaggtcta cccctgaaag gacaccaata acagcaccca taacaagatt tgaggtattt
     5041 cctaagcttt ttagaaaatc agttaaccaa ccagtaaagg ctgaaatggg accaccaatc
     5101 agataaatca tgataagact actcaataaa gctgcaaaaa atggaacaat aagtgttggc
     5161 atcagtcctt ttgcccaatt tggtactttt acatttttta aaatacctaa ggcgataaat
     5221 cctgcaatat agccacctaa aataccacca ataaaccctg cactgatagc gtttgcaatc
     5281 aatccgacaa caaaccctgg ggcaatccct ggtttaccag cgattgaaaa agcaatacca
     5341 gtcgcaataa taacaggtaa caaaccgaga gctttaccac ccattgtggc catcgtatcc
     5401 caaaaagaaa aattgccaat aactagtgca tcttgtgctc taccgcccat agccatacca
     5461 atagcgacta agaaacctgc tccacaaaca attggaatta aataagaaat tgctgttagc
     5521 aagtgacctt taaaattagc ctttttaatc atatctttca ttattatctc cttttacgaa
     5581 aatgcttcta taacatcatc ttcattttta gcattaatta atttttctac aatttgatca
     5641 tttcctagtt ttctagcaaa tagtgataat atttttaaat gttcttgtgc agcgattgaa
     5701 tcatttccta ctgcaaataa aacaataact tttacaccat tatcatctaa agtttcccat
     5761 gggatttctg aagaattaat cccaattacg actgcaggct ctgttacatg tgagctttta
     5821 ccatgcggaa tggcaatata atttccaatg ccagtttgtc cttcagactc tctaagatag
     5881 atgtctttta taaatcctcc ttcatcagaa acaaaccctt cattttttaa aagcgacact
     5941 aattctgaaa atgcttcatc ttttgtggtg acattcatat tagttttaat taatttacgt
     6001 ttaatagcat ctcttgcttc cattgggcaa ccctttctaa tttgacgcaa cttccacaag
     6061 ttttgcgatt aatttgttag gtgacttaat cgcaacttct gttggaactt ttactacttt
     6121 tttcccttca aatctttctt caccagcaat ttttacatca acagctaaca aaacaatatc
     6181 agctgctgct atttcttctg atgttagttg attttcaata ccaattgtcc cttgtgtttc
     6241 aatcttgaca tcatgaccag ctgctttcga agcattttcc aatttttctt gagcaatata
     6301 agtgtgagca attcctactg tacatgctgc tactccaaca attttcataa tatttcctcc
     6361 attaatcggc ttggtttaat aatttataaa cttctaattc ccgtgtttca ctttttaatt
     6421 gtcttaatac ttcttcatta cttattgttt tattaatttt caaaataatg tcttttacgt
     6481 ttttaatatc atctcttgac agcgcaatga gaaatactgt atcgaccaat gactctttcc
     6541 atttaatttt atgagtattt tgaataaaaa caaccactgt ttttaagaca aattctggcc
     6601 ttccatgcgg taaagcgact cccaatggaa actctgtact tcccattttt tccctatcaa
     6661 gtaaactagc aacaaattct ttttggacat atcccatttc tactagattt tgtcccacag
     6721 aatttaataa ttcttctttc gttttgaaat cttgattaag catcggaatg taatagttag
     6781 cttcaatttg atcttttttc ccaatagtca cgttattact ataagggttt cctgttaata
     6841 attgggtaat ttcttcattt tt
//
LOCUS       DATRSD010000087         6724 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRSD010000087 DATRSD010000000
VERSION     DATRSD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.088"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..300)
                     /locus_tag="VBD85_001701"
     CDS             complement(<1..300)
                     /locus_tag="VBD85_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO7134242.1"
                     /translation="MTVYNINHGIGWASSGVEYAQAYRGSVLRKLGIDAKFIFTDFFS
                     ADNIISLTRNIGFQDKEIIWLYNFFSDIELAPTTFSIEDLQKQYLGKLVRKEDKGR"
     gene            complement(314..2701)
                     /gene="secA2"
                     /locus_tag="VBD85_001702"
     CDS             complement(314..2701)
                     /gene="secA2"
                     /locus_tag="VBD85_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7134243.1"
                     /translation="MIAFNSLFSLDKKRLKKLQRTLNTINSLKGQMATLSNEELQAKT
                     TEFRKRLVNGETLDDICAEAFAVVREADKRVLGLFPYDVQVIGGLVLHQGNTAEMKTG
                     EGKTLTATMPLYLNALEGKGAMLLTNNSYLAIRDAEEMGKVYRFLGLSVGVGVSDNEE
                     EDRDAATKRAVYSSDIVYSTSSALGFDYLIDNLASSKSQKYMPKLHYAIVDEADAVLL
                     DMAQTPLVISGSPRVQSNLYKIADELILSFEEQVDYYFDKERQEVWIKNQGVREAERY
                     FRIPHFYKQSNRELVRHLNLSLKAHKLFERGKDYVVDDGEIKLLDATNGRVLEGTKLQ
                     GGVHQAIEQKEHLNVTPESRAMASITYQNLFRMFTKLAGMTGTGKTAEKEFIEVYDME
                     VVRIPTNSPVRRIDYPDKIYTTLPEKIHATIEFVKQVHDTGQPILLVAGSVRMSELFS
                     ELLLLSGIPHSLLNAQSAVKEAQMIAEAGQKGAVTVATNMAGRGTDIKLGKGVSELGG
                     LAVIGTERMKSQRMDLQLRGRSGRQGDIGFSQFFVSFEDDLMIESGPKWAQDYFRKNR
                     DKVNPEKPKALGQRRFQKLFQQTQEASDGKGESARSQTIEFDSSVQLQREYVYRERNA
                     LINGESGHFSPRQIIDTVISSFIAYLDGEVEKEELIFEVNRFIFDNMSYNLQGISKEM
                     SLEEIKNYLFKIADEILREKHNLLGDSFGDFERTAALKAIDEAWIEEVDYLQQLRTVA
                     TARQTAQRNPVFEYHKEAYKSYNIMKKEIREQTFRNLLLSEVSFNENGDLQIYFI"
     gene            complement(2688..3680)
                     /gene="asp3"
                     /locus_tag="VBD85_001703"
     CDS             complement(2688..3680)
                     /gene="asp3"
                     /locus_tag="VBD85_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO7134244.1"
                     /translation="MSTISYIYWDDFSRFSYNFGTKLQFLGKSVCFENPLAPSSTNLY
                     TWSSQTNYQSKRISPNLPLLRKGTRYSLSLNAELDLVSSLFVRIEFYNRFNESVGFEL
                     LKKDSIIFIYPKEAYTYTISLINAGCSDFTFHYLKLEEVTDSVIQEKNLSTEFTIEEH
                     QDVLNLLLVEKKDSVYINKIESISQLQQKVELVSNPSLNSDSLILPELEKGLEDALKV
                     FPNIKINVIAYGTQGNFAALYYAKKFPRITAYINDCFAPFGILLKSLPHLTAKQQIFL
                     REVWDTRETSPNVKHYGLVSENSSLNLVSMILSGNEHLPYLTLLKKQDDNYDSL"
     gene            complement(3677..5236)
                     /gene="asp2"
                     /locus_tag="VBD85_001704"
     CDS             complement(3677..5236)
                     /gene="asp2"
                     /locus_tag="VBD85_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7134245.1"
                     /translation="MSKIKLTILQVGEENWATKENIPNNMEWLFIKPDQISDFVTTEN
                     NYLTSSKLLQKLPRKISALLLTEQTYGPELSSLSSFFEVYEVFYPKDKHATGITEEFL
                     RSKMAQRYDSSSPDQLIRQFYKGLFIGQYGEKLQVSQIQIRNDFEGVVNYQGNNYLEL
                     EGQFGENYSFLLNFAYNIPFSSDFYNELFLEHIIEGDIDIRLVISLIVDGSVDDIAKE
                     WYFEKEDLNQLISLESDISGSLAVKLFAKGKGIVKLGPLHRRNGRGGLGTFLLGGERH
                     IDAIGHEFMTYFDPVDFKPPLTVYFSGFRSAEGFEGFWMMKSMKTPFMLICDPRLQGG
                     AFYIGSKEYEQKIVDAIQEKLAFLNFSSDQLILSGLSMGTYGATYHGAKLNPHAIIIG
                     KPIFNLGTVAQRERLERPDGFATSLDIQLLNQGDLTSSSSEKLNNYFWKSIEEGDFSN
                     TTFALAYMKNDDYDATAFSDLLQYFRGKKHKILGRGWDGRHGDCSAEVGAWFTSQYRR
                     MLSNDFGRKEE"
     gene            complement(5243..>6724)
                     /gene="asp1"
                     /locus_tag="VBD85_001705"
     CDS             complement(5243..>6724)
                     /gene="asp1"
                     /locus_tag="VBD85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7134246.1"
                     /translation="WYFKPTNVGFDDTINQMKMFDYAGKESRLVVLNYMPNLRYYLHR
                     YDLLESGYYSVFDDIQEIGNVRQQMIDFRQLNWPEGVDFTYTPFIVLVKKSGDLIAKV
                     QFGEEGNLTHIDYFANEQIAKKYLFDDRGFLSSILYYDNGGEAYQDYLAPSGERIMRE
                     YLREGDHHVEINPKKAIHFLKLSYSDIEELIREKYLTYLHKEVSKSDTIIVSFNQVHN
                     AFIVGNTSKGNLILSVFSERNNAHNVLEDYSSLSRADAIICDRLDIAAQLKEKIDKPV
                     VHVSPFDTRLALGKSNQVRDLEIYFVVDRLSHKELQKSLTSLYKVMLKNNDIKVTFVS
                     YEREFESRQLTYDYLKEATKVFDQKFFSLSDDDANQVLMQSDEVEKEKTRLSFTHPLS
                     ETDIINRLEYVRLIIDISKIPDLYTQIAGISSGIPQINTILTEFVEHRKNGYVIEEIQ
                     ELEKAIPYYCEQLTNWNRSLIYSIDKINDYTGGQLVERIINSY"
BASE COUNT         2168 a         1333 c          968 g         2255 t
ORIGIN      
        1 ccgaccttta tcttcttttc gaacaagttt tccaagatat tgtttttgca aatcttctat
       61 actaaaagtt gtcggggcaa gctcaatatc tgaaaaaaaa ttatataacc aaataatttc
      121 tttatcctga aaacctatat ttcgcgtgag gctaataata ttatcagctg aaaaaaaatc
      181 tgtaaaaata aacttagcat caattccaag ttttctcaaa acagaacctc ggtatgcttg
      241 agcgtactca acaccgctgc ttgcccaacc aattccatgg tttatattat aaactgtcat
      301 tatctttcat ctcttatata aaataaattt gaagatctcc attctcattg aaggatactt
      361 cactcaaaag tagatttcta aaagtttgtt cacgaatttc ttttttcatg atattgtagg
      421 atttatatgc ttctttatga tattcgaaaa ccgggttacg ctgtgccgtt tgacgagctg
      481 ttgccacagt acgtaattgt tgtagataat ctacttcttc aatccaagct tcatcaatcg
      541 cttttaaagc agcagtacgt tcaaagtcac caaaactatc accaagcaaa ttatgctttt
      601 ctcgaagaat ctcatcggct attttgaata aatagttttt aatttcttca agtgacatct
      661 ctttagaaat cccttgaaga ttgtaagaca tattatcaaa tataaaacga tttacttcaa
      721 aaatgagttc ttctttttct acttcaccat ctaaataagc aatgaaggaa gaaatcactg
      781 tatcaataat ctgtcttgga ctaaaatgtc cactttctcc attgatcaaa gcatttctct
      841 cacgataaac atactctcgc tgaagctgta cactcgaatc aaactctatc gtttgactac
      901 gtgcagactc tcccttaccg tcactagctt cctgagtttg ctggaataac ttctgaaatc
      961 ttctctgacc taaggcttta ggtttttctg gattaacttt gtcacgattt ttcctaaagt
     1021 aatcttgcgc ccatttagga cctgattcta tcattagatc atcttcaaat gatacaaaaa
     1081 attgactaaa accaatatcg ccttgtcgac ccgaacgtcc ccttaattgt aaatccattc
     1141 tttgactctt cattctctcc gttccaataa ctgcaagacc acctaattct gatactccct
     1201 tacctaactt aatatctgta ccacgaccag ccatattcgt tgcaacagtg accgcacctt
     1261 tttgccctgc ttctgcaatc atctgtgctt ccttaaccgc actttgagca tttaacaggc
     1321 tatgagggat accacttagt aataataatt ctgagaatag ctctgacatt ctcacagaac
     1381 cggcaactag taggattggt tgtcctgtat catgaacctg tttaacaaat tcaatcgttg
     1441 catggatttt ctctggtaag gttgtataaa tcttatcagg atagtcaatt cgtctaacag
     1501 gagaattagt tggaattcta actacttcca tatcatagac ctcaataaac tctttttcag
     1561 cagtttttcc agttcctgtc attccagcca atttagtaaa cattctaaaa agattttgat
     1621 aggtaatgga agccattgct cgagactctg gtgtgacatt aagatgctct ttttgctcaa
     1681 tagcttgatg aacacctcct tgaagtttag taccttctaa aacacggcca ttagtcgcat
     1741 ctagcaactt gatttcacca tcgtcaacca cataatcttt tcctcgttca aataatttat
     1801 gtgcttttaa agataagttc aaatgtcgca caagttctcg attagactgt ttataaaaat
     1861 gagggattct aaaatatctc tcagcttcac gaacaccctg gttttttatc cacacttcct
     1921 gacgctcttt gtcaaaataa taatcaactt gttcttcaaa actaagaatt agttcatctg
     1981 ctattttata gagattagat tgtactcgag gtgaacctga aataactaaa ggagtctggg
     2041 ccatatctag aagaactgca tcagcttcat ctacaatagc ataatgtaac ttgggcatat
     2101 atttttgaga tttagatgaa gctaggttat caattaaata atcaaagcct agtgcactac
     2161 ttgtactata gacaatatcc gaactataaa cagctcgctt agtcgcagca tctctatcct
     2221 cttcctcatt atcagaaact cccacaccga cactcaatcc taaaaaacga tatacttttc
     2281 ccatttcttc cgcatctcga atagcaaggt aggaattatt agtcagcaac attgccccct
     2341 tcccttctaa agcattgaga tacaaaggca tagtggcagt taatgttttt ccttcaccag
     2401 ttttcatctc agcagtattt ccttggtgca gaacaagacc accgattacc tgtacatcat
     2461 acggaaatag acctaatacg cgcttatcag cttccctaac aacagcaaaa gcttcagcac
     2521 aaatatcatc tagtgtttcc ccattaacta aacgttttct aaattcagtt gtcttagctt
     2581 gcaactcctc atttgatagt gttgccattt ggccctttaa actattgata gtatttaaag
     2641 tacgctgaag tttttttaaa cgtttcttat ctaacgaaaa taaactatta aaggctatca
     2701 taattgtcgt cctgcttttt taaaagtgtg aggtaaggta gatgttcatt accagataag
     2761 atcattgata ctaaatttaa agaggaattt tctgaaacca atccatagtg ctttacattt
     2821 ggagacgtct ctctcgtatc ccagacttct ctaagaaata tttgttgttt ggcagtcaag
     2881 tgtggtaagg atttaagtaa aattccgaaa ggagcaaaac aatcattaat ataggcagtt
     2941 attctgggaa attttttagc ataatataaa gcagcaaaat taccttgtgt tccatacgca
     3001 atgacattaa tcttaatgtt aggaaaaact tttaatgcat cttctaaccc cttttccaac
     3061 tcaggtaata tcaaactatc agaatttaat gagggattgc tgaccaactc gactttctgt
     3121 tgcaattgag aaatgctttc tattttatta atatatacac tatctttttt ctcgaccaaa
     3181 agaagattta aaacgtcttg atgttcttca atagtaaact ctgtcgatag atttttctcc
     3241 tgaataacgc tatctgtaac ttcctcaagt ttcaaataat gaaaagtaaa atcgctacaa
     3301 cctgcattaa tcaaactaat ggtataggta taagcctctt taggatagat aaaaataata
     3361 ctatcttttt ttaacagttc aaaaccaaca gactcattaa agcgattgta aaattcaatg
     3421 cgaacaaaaa gagagctaac taaatcgagt tctgcattta atgacaagct ataacgagtt
     3481 ccctttctta gtaaaggtaa gttaggagaa attctttttg actgataatt agtctgggag
     3541 ctccatgtat acaagtttgt gctagaagga gctaatggat tttcaaagca tactgatttt
     3601 cctaaaaatt gtaattttgt tccaaaatta taagaaaacc ttgaaaaatc atcccaataa
     3661 atgtagctta tcgtactcat tcttctttcc ttccaaaatc attagatagc attcgcctat
     3721 actgacttgt aaaccatgca ccaacttctg cagaacaatc accatgtcgg ccatcccaac
     3781 ctctgcctaa tatcttatgc ttctttcctc taaaatattg caataaatca gaaaaggcag
     3841 tcgcatcata gtcatcgttt ttcatatagg ctaaggcaaa agtcgtattt gaaaaatctc
     3901 cttcttctat agatttccaa aaatagttat ttaacttctc agaagaggaa ctggtcaagt
     3961 caccttgatt gagtagttga atatctaatg aagtagcaaa tccatctggt ctctctaatc
     4021 tttcacgttg agcaactgtt cctaaattaa atattggttt accaataata atagcatgag
     4081 gatttagctt agcaccatgg taagtagcac cataagtccc cattgaaagt ccggataaaa
     4141 ttaactgatc agaactaaaa tttaaaaagg ccagtttttc ttgaatagca tcaactattt
     4201 tttgttcata ttctttgctt cctatataaa aggcaccacc ttgtaatcgt ggatcacaaa
     4261 ttaacatgaa tggagtcttc atactcttca tcatccagaa tccctcaaag ccttcagctg
     4321 aacgaaatcc cgaaaaataa acagttagag gtggtttgaa atctactgga tcaaaataag
     4381 tcataaattc atgacctata gcatctatgt gacgttctcc tcctagtaga aatgtgccca
     4441 atcctcctct accattacga cggtgcaatg gacctaactt aacgataccc tttcccttag
     4501 caaaaagttt aactgcaagg ctaccactaa tatcggactc taaagaaata agttgattta
     4561 ggtcttcctt ctcaaagtac cactctttag caatatcatc aacagaacca tcaacaatca
     4621 acgagataac caatcgaata tctatatcac cctctataat gtgttctaaa aataactcat
     4681 tataaaaatc cgatgaaaat ggaatattgt aagcaaaatt aagcaaaaaa ctatagttct
     4741 caccaaattg cccttctaat tcaagatagt tatttccctg atagtttact acaccttcaa
     4801 aatcatttct aatttgtatt tgtgaaactt gaagtttctc tccgtattgt cctataaaaa
     4861 gccctttata aaattgacga attagctgat ctggagagct actatcatat cgttgggcca
     4921 ttttcgagcg taaaaactct tctgttatcc ccgtagcatg tttatcttta ggataaaata
     4981 cttcataaac ctcaaagaat gacgataaac tagataactc tggtccataa gtttgctctg
     5041 tcaacaaaag agcagatatt tttcgaggta gtttttgcaa taactttgac gatgtaagat
     5101 aattattttc tgtagttaca aagtcagaaa tttgatctgg tttaataaag agccattcca
     5161 tattattagg gatattctct ttagttgccc agttctcttc accaacttga agaattgtaa
     5221 gttttatttt tgacatattt atttaataac tatttataat tctttctacc aattgaccac
     5281 ctgtatagtc attgatttta tcaatagaat aaattaaaga acggttccaa ttagttaatt
     5341 gttcacaata ataaggaatt gccttttcca attcttggat ctcttcaata acgtaaccgt
     5401 ttttcctgtg ttcaacaaat tctgttaata ttgtatttat ctgagggata cctgaactta
     5461 tccctgcaat ttgcgtgtat aagtctggta ttttcgagat atcaataatc aaacggacat
     5521 actctaaccg gtttatgata tctgtttctg acaaaggatg ggtaaaagaa aggcgtgttt
     5581 tttccttttc tacttcatca gattgcataa gaacttgatt ggcatcatcg tctgataaac
     5641 taaagaattt ctgatcaaaa acttttgttg cttctttaag gtagtcatat gtcaattgcc
     5701 gactttcaaa ttctcgttcg tagctgacaa atgttacttt tatatcatta tttttgagca
     5761 taaccttata taaggatgtt aaagactttt gcaattcttt atgggaaagt ctatctacga
     5821 caaaatatat ttctaaatct ctaacttgat tactttttcc caaagctaaa cgcgtatcaa
     5881 agggagaaac atgaacaact ggtttatcta ttttttcttt caattgtgca gcaatatcta
     5941 gtctatcaca aataatagcg tctgctcgag ataaggaaga gtaatcctcc aatacattgt
     6001 gagcattatt cctctccgag aaaacagata atattaaatt tcctttacta gtgttcccaa
     6061 caataaatgc gttatgaact tgattaaagc taacaatgat agtatctgat ttactaactt
     6121 ctttatggag ataggtcaga tatttctctc taatcaactc ttcaatatcg gagtaagata
     6181 actttaaaaa atgaatagct ttcttaggat taatttcaac atggtggtca ccttctctta
     6241 ggtattctct catgatcctt tcccctgatg gtgctaaata atcttgatag gcttcaccac
     6301 cattatcata atagagaata ctagatagaa atcctctatc atcaaagagg tatttcttag
     6361 ctatttgttc atttgcaaaa taatcaatat gcgttaaatt tccttcttca ccaaattgaa
     6421 ccttagcaat taaatctcct gactttttta ccaaaactat aaacggagta taggtaaagt
     6481 ccaccccttc aggccaattg agttgtcgga agtcaatcat ttgctgtctt acattcccaa
     6541 tttcttgaat atcatcaaaa actgaataat aacctgattc taacaagtca tagcgatgta
     6601 aataataacg taaattgggc atatagttta agacaactaa tctactctct tttccagcat
     6661 aatcaaacat cttcatttgg ttgatagtat catcaaaacc gacattagtc ggcttgaaat
     6721 acca
//
LOCUS       DATRSD010000088         6499 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRSD010000088 DATRSD010000000
VERSION     DATRSD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.089"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(442..2418)
                     /gene="ftsH"
                     /locus_tag="VBD85_001706"
     CDS             complement(442..2418)
                     /gene="ftsH"
                     /locus_tag="VBD85_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO7134247.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMIAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2441..2983)
                     /gene="hpt"
                     /locus_tag="VBD85_001707"
     CDS             complement(2441..2983)
                     /gene="hpt"
                     /locus_tag="VBD85_001707"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO7134248.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2988..4262)
                     /gene="tilS"
                     /locus_tag="VBD85_001708"
     CDS             complement(2988..4262)
                     /gene="tilS"
                     /locus_tag="VBD85_001708"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7134249.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNVYFPALERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            complement(4264..5550)
                     /locus_tag="VBD85_001709"
     CDS             complement(4264..5550)
                     /locus_tag="VBD85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7134250.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIVSLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRIAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(5550..5684)
                     /locus_tag="VBD85_001710"
     CDS             complement(5550..5684)
                     /locus_tag="VBD85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO7134251.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            complement(5687..6058)
                     /locus_tag="VBD85_001711"
     CDS             complement(5687..6058)
                     /locus_tag="VBD85_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO7134252.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(6045..6317)
                     /locus_tag="VBD85_001712"
     CDS             complement(6045..6317)
                     /locus_tag="VBD85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7134253.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT         1973 a         1236 c          930 g         2360 t
ORIGIN      
        1 gtcattgacg gatttacaat gtaaatccac cctgcacatt cgttcgtctt gttcagtttt
       61 caaaggtctt tgcctctctt gagacaactt ctatattcta gcaaacttat tctgctttgt
      121 caactacttt ttttaagttg ttaactatgc gctactagaa actgctctct tgagacaact
      181 tattcattat actaaatatt tctacttcct gtcaatacta tttttgcatt ttttctttta
      241 tttttaaaaa gttaatatta tttatagtaa ctaaccttct atacttgttt aatggatagc
      301 atttttaccg ttgtcatgtt catatttcat cttcttaatt cacaaattta aacttcatct
      361 tcataaaaaa cacccctcaa attttatcta aatttgaagg gtgtttgaaa tttataaagt
      421 tctttaaaaa tatatgatga cttatttttt atcttcttct tgcatttttt ctttgatttc
      481 atcgtatgat aacgctcttg ctttatcttc atcattttct gtctcaggca ttttacctgt
      541 ctcgaaaatc gatttaatct gagcagcatc aagagtctca tattttaaga gggcttctgc
      601 aattaattta tgagtatcac ggttttcgtt gataatatca gctgccttat tacgtgcttc
      661 attaagaagg tgacgaactt catcatcaat aagttgtgca gtttgagcag aatatgattt
      721 ttcaggtgac atttggccag caatcattgc atggttacct tcgtattgaa ctgggccaag
      781 tttctcactc ataccatatt cagttaccat agcgcgggcc atggcagtgg cttgttcaaa
      841 gtcatttgag gcacctgttg tctgagcgtt gaaaataatt tcttccgcta cacgtcctcc
      901 cataagacct gctaattgct ctttcatatc atcttttgaa agaagcattt gatcttcttt
      961 aggtaaagca atcatatatc cacctgcacg accacgtggt acgatagtaa ctttatgaac
     1021 aactcgcgca tttgaaagaa tcaaaccgac aattgtgtgc ccagcttcat ggtaagcaac
     1081 catagctctt tctctttcag aaatagtacg atctttttta gaaggaccag caattacacg
     1141 gtcttctgct tcatcaatat ctgaagcatc aataactttt ttatttcgtc gcgcagcaac
     1201 taaagcagct tcattgagaa cattctccaa atcagcacca acaaatcctg gggtttgttg
     1261 agctactact tttaagtcaa cattatctgc taatggttta tttttagcat gtactctcag
     1321 aattgcttca cgacctttta catcaggttg tccaactaga acttttctat caaaacgtcc
     1381 tggacgcagt aatgcgggat ctaacacgtc actacggttg gtagcagcga taacgataat
     1441 actttcgttt ccttcaaaac catccatttc aattaaaagt tggttgagcg tttgttcacg
     1501 ttcatcgttt ccgccaccca taccagctcc acgtcgacgt ccaacagcat caatttcatc
     1561 aatgaaaata atagcacgtt cagctttttt agcgtcttca aacaaagagc gtactcgact
     1621 agctccaaca ccgacaaaca tttcaacaaa atctgaaccg gaaatactaa agaatggaac
     1681 ccctgcttca ccggcaacag cttttgctaa cagagtttta cctgtccctg gaggtccttc
     1741 taatagaacc cctgctggaa ttcgggcacc tagagattta tatcgtttag gatctttaag
     1801 gaagtcaacg acttctatta attcttgctt ttcttcctct gctccagcaa catccgaaaa
     1861 tcgaacttta acctcatctt tactagatga tcttgcttta tttttaccaa agctcatggc
     1921 accacgagcg ccaccgccac cgccttggtt catcatcatc ataaagaaac caatcatgat
     1981 aactaaaggt aaaaaactgg caatataaga aatccatgtc ccacttgaac tttcatgttt
     2041 gacctgaata ttagtgttat tttcttcagc agcgctaaca agagatttta ttgaagagtc
     2101 atttggtagt ataacagaat taaatcctgt aactttagta gcaacactac cacctaaaaa
     2161 agtaaaacta ttagctgatt taattgtttt ggctttctta tatgtcccac taacttcaac
     2221 aacaccacca cttggctggt atgaaataga cttaatttct ccagctttta attgtttaac
     2281 aagttttgta taagaaattt gctggttttg agagctagtt ccttttaaat agtattgaaa
     2341 ggttgtaata accgcaataa tcaataatat gtaaataaag gaatttttca gaaaaccatt
     2401 atttttatta tttttcatag tttaagagag tacctttttt ctattttgag taaatttctt
     2461 cttttaacac accgacataa ggtaggttac gatagttttc agcatagtct aaaccaaatc
     2521 ccacaataaa ttcatttggg atatcgtaac aaacgtaatc agcatcaata tctaccaatc
     2581 gcccttctgg cttatcaaaa agggttgcaa ctttgacaga gttagcttga cgatacttaa
     2641 acatatctcg aaggtatttt aatgtacgtc cagtatcaat gatgtcttca ataaagatta
     2701 catctcttcc ttcaatgtta gtatcgacat cttttaaaat tttaacttct ccactactag
     2761 ttgtaccacc atgatagctt gaaacaacca taaaatcaat ttcaacatgt gtatcaatat
     2821 gctttaatag ttcagccata aaaggcactg acccctttaa tacacctact aataatggat
     2881 tcttacctgc ataatctgca gttaatttag ctcctaattc tttcgttttc agaataatat
     2941 cttcttctga atataaaact ttttttatat cattttcaag catgatgtca ccttttttct
     3001 aaattttcaa tataaagttt gcccttcatt atatcatgtt cagaaggttt tctcaaatat
     3061 gttctacctg caacaatcac aaatatcaac tctttatttt gctccccaat aacagcttct
     3121 tctctgtctt ttagagttat tttctcatca ataaatagac ggcgaatttt tttagtgtga
     3181 tttcctaaaa atatcctatc cccttctttt cttcttctta aaataatcgg agaagtatca
     3241 taaataggaa tagtaatgtc tgcttgagaa atgaattttc cataggaaaa taaatattgt
     3301 gagaatattt gtatattatg caattctaat cttttttcaa ccttaaaact atccgtctta
     3361 ggatggattt ttgtaatatg aaaactctcg taatctttat gaagaaaata gcccgattta
     3421 atagtatagt caatattggc tttagttcta atcaatttta atattactct gaactgttct
     3481 ttactcactt gtaaatctgg aaatttgcta atgtaatctt gcaataaaaa ttcttggaca
     3541 gaatgagttt gccttaaaaa atcatagcag tttgttactt cgatgctcct tgttaggtct
     3601 gttaaagccg tgaataaaca ctctgtttcc tcagagagag ttatgagaga atctttcaat
     3661 tgattatttt ctctttccaa agcaggaaag tagacgtttc ttattcgatt tctcaaataa
     3721 tcagacgaag cattgctagc atcttcaaaa tgaaaaatat ttggtaataa ctccttttta
     3781 tatggaagaa atggtctaat caattgaccg tttgcaaaag cactcacttg cttaatccct
     3841 gataaatagc gtagcctgct tcctcttaaa attctcataa atacagtttc agcttgatca
     3901 tcagcatggt gagctgttac taaagccgtg taaccttctt cacgcataac ttttgaaaaa
     3961 aaattgtagc gatggttacg tgctctatct tctgaaaata ttccttgaaa ataagagata
     4021 aagacaggga catcatgtat ctgtccccac tgtctgatat attcttcttc tttttctgat
     4081 tcttttcgct gtccatgatt tatgtgtgca ataccaatac taattgataa ctctttttga
     4141 tactggtata aaaattgcaa caaatttatt gaatcaatgc caccagaaac agcaattaga
     4201 actttctgat gagcggtaaa taaccctttg gataaggtat ctttcaaaat agtgttatac
     4261 atattatttt aaaacctggt aaacgtcgtc agcaatctta gtgatatcat cgtaagatga
     4321 tttatccgta aagataatca taataaaagg ttgttctgca taaacaatag ccgcatcatg
     4381 tttgtagtca taagcatctc caattttatg tgctacttta actggtatat ttttagggat
     4441 tcttgtatta tcaaaatcag tctttgatag gtaattaaca atatctccat tttgataata
     4501 aattgcttcc atcattttac ccgcagcttg tggagaaatc tggcgtttct tcatatccca
     4561 gtgcatacca gaaattgtgt ctacttgtgt ttgaaacatt gaatcgtatt gattattgac
     4621 ataataacct agcatatttg ttgcaacatt atctgattcc ttggcagttg cttcaagtaa
     4681 ctccttaact gtatataatt tatgatcagc ttttttctct aatttcccac taccacttgg
     4741 atcatagcta ttaggaaatt gattaacatt atctttgtat tcaaatttgc tgtccaatga
     4801 cagttttcct aattttactt gctcttggct agcataaagt gttgctaatt ttgcaatact
     4861 tgcagagtac attgacctat caatattaat accagctact gtctgtgtat ttaattgttt
     4921 gatgtatata ccatatttat ctttattata tttttccaat aatacttctt gaaccttagc
     4981 tatacgatta tcagcaaccg atagatcttc tactgatatc caaaaaccac tgtccgtttt
     5041 ataataaata ccatgttctg tttctgcttg ttctactaaa tgcactctgg aataaggttt
     5101 taaattattt ttaacttcac taacaccatt tgtgtaaggt gcttcataag tcacaaaaga
     5161 aggttttaac caataatcca atgagacaga ttgctgctta gaaactaaat cattaaaaat
     5221 agcttcacga ctagccttta tgaactgtcc attggacaaa gtaaatactg gaattccatt
     5281 tgaattaatt gtaagggaaa caatcgacaa taaagtattt ggaactaaag tctctcctgt
     5341 tatggttaaa gcttcatctt gaaaactgct tatttcaatt tttgtagttg gtctctctgg
     5401 aatagcatca aaatacattt ccttatccac aactgtctga gttaaggcgt atcgtctatt
     5461 ttgagaaagc gttaatggct cctcggtact tatagctggg attgatacaa aatataagct
     5521 acataataag aaggttaaaa ctttcttcat tacttttctc tgctctcaat cttttctttt
     5581 tctacaattg tttgcctaag ttgatagttc ttatcagcta actcttctag tttgtcatcc
     5641 gagtaggcta gaaaatgttc tattgtttca attaaattat tcataattat tttggtaaaa
     5701 ggtctggtaa tgggtaaatc atttcgccgg tcttagagaa ataatactta gctcgagcat
     5761 atttttgaac gtaatctgga ttttttagtt gttttgctag taacttctgg ttctcagttc
     5821 tattagttaa tgtctgatag tctttcgtta attttacaac ttcttgacga cgttcttgta
     5881 aagttctgta actcttaact aaattataag tgggtaaaat aaataaaagc atgacaaaaa
     5941 taagaaccca acccattaaa cgattttttc ggcgtaactc ctcagcttcg taacgttttt
     6001 ttagattctc atcgttaata tattgattat ttaactgaac aacattaggc ttgctcatct
     6061 gtttctatcc ttgtttcatt aataatttca tacatcttga tagcatcttc tttcttagtg
     6121 ctatctttca tttctaatac tttaacggtc aacaatttat taccaaaacg aatttcaact
     6181 tggtcattaa gctttaggtc agttgatgac tttgctaata cgccattcac cttgacacgt
     6241 cctttgtctg caacttcttt agcaacggga cgacgcttaa tgatacgcga cacttttaga
     6301 tacttgtcta atctcactga tgatcacatc cttctatcta aattaactct actattttag
     6361 catttttttt gaaaaatatc tgaactgata atatttgtca atataatgta acaaaaagag
     6421 agctaatagc tctcttaatt ctctcaaata tcaagtaaaa aacttctatt ttaataacct
     6481 ctaaaaatat ttatgtctt
//
LOCUS       DATRSD010000089         6102 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRSD010000089 DATRSD010000000
VERSION     DATRSD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.090"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            159..980
                     /locus_tag="VBD85_001713"
     CDS             159..980
                     /locus_tag="VBD85_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7134254.1"
                     /translation="MIKVIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1081..1695
                     /locus_tag="VBD85_001714"
     CDS             1081..1695
                     /locus_tag="VBD85_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO7134255.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDTYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            1858..3294
                     /locus_tag="VBD85_001715"
     CDS             1858..3294
                     /locus_tag="VBD85_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO7134256.1"
                     /translation="MVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGEDR
                     FAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFPK
                     GDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFYE
                     RLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELVA
                     SAIAVKIAHEVDPNNQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQTRGKY
                     PNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENIF
                     ASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVED
                     DYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNNK
                     GEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3385..4518
                     /locus_tag="VBD85_001716"
     CDS             3385..4518
                     /locus_tag="VBD85_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7134257.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLTAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            4614..5294
                     /gene="radC"
                     /locus_tag="VBD85_001717"
     CDS             4614..5294
                     /gene="radC"
                     /locus_tag="VBD85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO7134258.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(5307..5945)
                     /locus_tag="VBD85_001718"
     CDS             complement(5307..5945)
                     /locus_tag="VBD85_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO7134259.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
BASE COUNT         2009 a          904 c         1155 g         2034 t
ORIGIN      
        1 agtataggta aaagtcgatt taatctctga aacaaaattt aacttaatta atagaaccgt
       61 tgacgtatca ggtatcaaat ctttttggtt tatatgtgat ataatgaaag aacatattga
      121 gatatttggc accctcttga aaggcaaagg ttacttgtat gattaaagta atagcaacag
      181 atatggatgg aacttttcta cgctcagata aaacttatga taaagcacgg ttttcttctt
      241 tattaacgtt gatggagaag tatgatatta aatttgtagc tgctagtggt aatttatacg
      301 atcaattgtt gttaaacttt ttggaatacc ctaatcgtat cgcctacgtc gctgaaaatg
      361 gtggtcgtgt cattgaccaa gatggcacat tactaaaaga aacttattta tctaatgata
      421 cagttgcagc tgttttatcc tatttatatc agaactatcc tgagacactc atcagcttat
      481 ctggtgaaaa acgttcatac cttgagcgaa gaactcctat taataggcgg actgaattag
      541 aatactatat gcctaatttt atctataaag accatttgct gcccttagat gatgaccgat
      601 attttcaaat gacactttgg gtaaatgaaa atcttgtttc tgagatgttg ttagatatta
      661 gtgaacactt taaaaatcat catatcagat tgacctctag tggttttggt tgtatcgatg
      721 tcttaccagc tgatgtcaat aaagcggatg gtatcgctat tctccttgaa aagtggggat
      781 tgaaacagga tcaagtgatg gtttttggtg atgggggcaa tgatgtggaa atgttgagag
      841 cagcaaatat ttcttacgct atgtcaaatg cgccagaaga aattaaagct attgctaaat
      901 atcaaacagt tagtaacgat caagatggtg tgttagaaac gatagagaac tttcttcaca
      961 agatgagtga taatgaatag aaccacgtag cttttcacga agcatactat tcaattctca
     1021 ggtgaaattt tgttataata caagaaagcc tagagaaata tttaagaatg aaggagaacg
     1081 atgttagaag ttattgacaa agctttaaga gattatcaaa tgaaacgaga acagtttttt
     1141 gagattaaca atcagacagt acaagaaggt gccattgttt tcacaggaga ttctatcgtt
     1201 gagttttttc ctttaaaaaa acatttagga cgtgactatc ctttagttaa tagaggagta
     1261 gcaggatcag atacttattg gcttttagaa aacttgagaa cccaagtatg ggaactatta
     1321 ccatccaaag tatttatttt aattggaaca aatgatattg gtttagggca ttcacagtca
     1381 gaaatcatag ctaatatcac ggatataatt gcagaaattc gtgcggaatc ttatatgaca
     1441 gagattaata tactgagtgt tttacctgtt agtgaagaag atgactatat agagcgtgtc
     1501 aaggtaagaa ataatcaagc aataaaagct ttaaacaaaa ccttatcagt aatttctggc
     1561 attaattata ttgaattgta tgatttatta gttgacgaaa aaggtcagct tgctagcagt
     1621 tttacaaaag atggtttgca cctaacagat caagcctatg ctaaaatttc tgaaacgata
     1681 aaactccacc tttaaaagtg tttagatact atttttaagt atttaaatac acagaattag
     1741 tgaaaagacc ttctaagtag aaagttcatg tcacggatag taccagtatt tggcgaatca
     1801 atgtcagtaa ctggtctttt ttaatattat gataagaaaa aacaagaggg gtaaatgatg
     1861 gtaaaacaag tatttccaaa aggtttttta tggggcgggg caactgctgc caatcaatgt
     1921 gaaggagctt acaatgttga tggacgtggc ctagcaaatg tagatgttgt tcctactgga
     1981 gaagatcgat ttgcaattat ttcaggacaa aagaaaatgt ttgattttga ggaaggatac
     2041 ttttatccag caaaagaatc aatagatttt tatcatcatt ataaagaaga ccttgcatta
     2101 cttgcagaaa tgggtttcaa aacctatcgt atgtcaatag catggacgcg tatttttcca
     2161 aagggtgatg agctatatcc aaatgaagct ggtcttcggt tttatgaaaa tatttttaaa
     2221 gagtgtcgta agtatggtat tgaacctttg gtaaccatca cacattttga ctgtcctatc
     2281 taccttatta aacattacgg tgggtggcgg agccgtaaaa tgattggttt ttatgagcgc
     2341 cttgtacgag ctttatttac tcgttttaag gggttagtta aatattggtt gacctttaac
     2401 gaaatcaata tgattttaca cgctcctttt atgggcgctg gcttatattt tgaagatggt
     2461 gaaaatcaag agcaaattaa atatcaagca gctcaccatg agttagtggc ttcggctatt
     2521 gcagtaaaaa ttgctcatga agttgatcca aataatcaaa tcggatgtat gttagctgca
     2581 ggtcaatact atccaaatac gtgtcatcca caagattatt gggcttcgat gcaaaaaaat
     2641 agagaaaatt atttttttat tgatgtgcaa acgcgtggca aataccctaa ttatgctaaa
     2701 aaatattttg agcatttagg tatttcaatt caaatgacag cagaagatct tgctttgtta
     2761 agagactata cggttgattt tatttcattt tcttactatt caagccgagt agcttcaggt
     2821 aatccaactg tcagtgaaca ggttcaagaa aatattttcg catctcttaa gaatccttac
     2881 ttgaaatctt ctgaatgggg atggcaaatt gatccgcttg gattacgcat taccttaaat
     2941 gctatctggg atcgttatca aaaaccgatg tttattgttg aaaatggact tggagcagta
     3001 gatattcctg atgagaatgg ttacgtagaa gatgattatc gtattgatta cctccgtcag
     3061 cacattgctg caatgagaga tgctatatat gtagatggtg ttaatctaat tggctatact
     3121 acatggggat gtattgattt agtatctgca ggaactggtg aaatggaaaa acgctatggt
     3181 ttcatttatg ttgatcgcaa taataaagga gagggaacac tcaagcgtta caagaaaaaa
     3241 tcgttttatt ggtacaagaa ggttattgca agtaatggca gtcaaataga ataaagagca
     3301 gactaataac tgttaactct ctttttctct ttcataaaat agtgtataat aattgaaaga
     3361 aattaagatt aataaggaga tttcatgaaa tttgaaaaac ggcaggtcta ttatgttgtc
     3421 ataacatttg ctatttgcta tgctatacag gcttattggg gagctgtttc taatatttta
     3481 actacgcttc ataaggcaat atttcctttt ttgatgggag ctggaattgc ctatattatt
     3541 aatattgtaa tgtcagtcta tgagcgatta tatataaagc tttttaaagg atctagacta
     3601 ttaatggcaa tcaagcgtag tgtttctatg attttatcct atgcaacttt tattggttta
     3661 attgtctggc tattttcaat tgtcattcca gatttgattt ctagtttgag ttctttattg
     3721 gttattgata ccggagcact tgctaaattg gttaataatc tcaatgaaaa taaacaaatt
     3781 tctgaggctt taaattatat gggaacagat aaagacttag tttctacttt aagtggttat
     3841 agccagcaga ttttgaagca agttttatct gttttaacaa atttactaac ctcagtttcc
     3901 tctattgcgg caacacttct gaatgttttt gttagtttta ttttttcaat ttacgttttg
     3961 gcaaacaagg agcagttggg acgtcaattt aatttgttaa ttgataccta tttaggttca
     4021 acaggcaaaa cattccatta cgttcgtcat atccttcatc aacgtttcca tggttttttt
     4081 gtaagccaaa ctttagaagc tatgatttta ggaagtttga cggttattgg tatgttgatt
     4141 ttccaatttc cttatgcttt aacagttggg gttttagttg cttttacagc tctaataccg
     4201 gttgtgggag cctacattgg tgttacaatc ggtttcatct taattgctac tgaatcgctt
     4261 actgaagcat tcttgtttgt tcttttcttg atccttttac aacaatttga gggaaatgtc
     4321 atttatccga aagttgtcgg tggatcgatt ggactgcctt ctatgtgggt tttaacggct
     4381 attactatcg gaggtgcttt atgggggatc ttaggcatgt tacttgctgt tcctgttgca
     4441 gctactatct atcagattgt aaaagatcat attatcaagc gacaaacgct tagaaatcgt
     4501 gcacgaacct atcgttaaat aaaaaatact tagatttttc atctaagtat tttttattga
     4561 tttaaaacat gagtttttga ttggtttacg aatattatga ctggaggtgt tttatgtacc
     4621 atattgaatt aaaaaaggaa gctttactac caagagaacg cctagttgat ttaggcgcag
     4681 atagattgag taatcaggag ttattagcca ttctcttacg tacaggtatt aaagaaaaac
     4741 ctgttcttga aatttcaacg caaattttag aaaacataag cagtttagca gattttggtc
     4801 aattatcctt acaggagttg caatccatta aaggaatcgg tcaggttaaa tccgtcgaaa
     4861 taaaagctat gctagaacta gcaaaacgga ttcacaaagc tgaatatgat cgtaaagagc
     4921 aaattttaag tagtgaacaa ttagcgagga aaatgatgct cgaattaggg gataaaaaac
     4981 aagaacattt agtagctatt tatatggata cacaaaatcg tattatcgaa cagagaacta
     5041 tttttattgg tactgtacgt cgttcagtag cagagccaag agaaattcta cattatgctt
     5101 gtaaaaacat ggcaacttct ttgattatta tacataatca tccctcaggt tctccaaatc
     5161 ccagtgaaag tgatttaagt ttcactaaaa aaataaaacg atcatgtgat catctgggaa
     5221 ttgtctgcct agatcacatc atcgttggaa aaaataaata ttatagtttt cgagaagaag
     5281 cagatatttt ataaataatg aactatttat cgctgctggt tcatgaaata aagtaaagtt
     5341 tgtaattcgc ttgttaaatc tacatactga acaatgatat cttttggtaa tgttaaatga
     5401 actggagaaa aactcaagat gccttttata cctgctttga ctaagatgtc agcaacttct
     5461 tgggcttctg tactaggtac tgttaggata gcagtttcaa tatcactatc tattaaatgg
     5521 tcattgatag tcgaaatacc gtagacagga atgccatcct cggttgtttt accaactaaa
     5581 tcattgctat ctaaatcaaa agccattgaa atttgcattt tattgcgatc gtggaaacga
     5641 taatgcaaga gagctctacc gatatttcca caccccacca gcataacatt tgttgtagaa
     5701 tgatcgttca atatttctgc aaagaagttc ataagttttt tgacatcata accaaaacca
     5761 cggcgtccta gttcaccaaa ataagaaaaa tcacgtcgaa cagtagcaga atcgatacct
     5821 agggcatctg caatttgttt ggaactagct ttttcgatgc catcagtatt aaaacgttta
     5881 aaaatacggt agtacagtga taaacgttta gcagttgctt taggaataga cttatccata
     5941 atcacaaaat tatctccttg ttaaaaattt tattattcga tttagaatca gttatataac
     6001 ttaattcttc acatctctat tgtacaaaaa gattgtgaaa gtgacaacaa aatggttcgc
     6061 aaaaagacca ctaaaatgtt aaaaatagtg gtcggtaaat ta
//
LOCUS       DATRSD010000090         5954 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRSD010000090 DATRSD010000000
VERSION     DATRSD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.091"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..172
                     /locus_tag="VBD85_001719"
     CDS             <1..172
                     /locus_tag="VBD85_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HEO7134260.1"
                     /translation="DLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKY
                     CLGRAVSKHYRF"
     gene            224..901
                     /locus_tag="VBD85_001720"
     CDS             224..901
                     /locus_tag="VBD85_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO7134261.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            894..2339
                     /locus_tag="VBD85_001721"
     CDS             894..2339
                     /locus_tag="VBD85_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7134262.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLGPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2346..4505
                     /locus_tag="VBD85_001722"
     CDS             2346..4505
                     /locus_tag="VBD85_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134263.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKASKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDLGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            4645..>5954
                     /locus_tag="VBD85_001723"
     CDS             4645..>5954
                     /locus_tag="VBD85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7134264.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNERK
                     KSLTRNKEKIPISIIVPAYNESTTIVSSIDSLLHLDYEAYEIIVVDDGSSDNTSDVLK
                     EEFALMKISNTIDSIIATQTCKDVFQRQVGKVKLTLIVKENGGKGDALNMGINAANYD
                     YFLCLDADSMLQVDSLSQISKSIQVDPTVIAVGGLVQVAQGVKIEQGKVASYRLPWRI
                     IPCAQALEYDSSFLGARIFLDYLRANLIISGAFGLFKKDLVKAVGGYDTQTLGEDMEL
                     VMKLHFFCRNNNIPYRICYETDAVCWSQAPTNLGDLRKQRRRWYLGLYQCLKKYKSIF
                     ANYRFGAVGSISYIYYILFELLTPFIECFGIVIIFLSLLFNQLNIPFFISLVSLYIFY
                     CVLITLSSFLHRIYSQQLVIGILDIVKVFYIAVFRYLILHPVLT"
BASE COUNT         2007 a          860 c         1143 g         1944 t
ORIGIN      
        1 ggatttaaat caaaattatt tattagatcc ttatcttgtt attttagaag ttaaatataa
       61 tggattttta ttgtcttata ttaaagaact cttagattcg gtagatagaa gtgaactttc
      121 agcgagtaag tattgtttag gaagagctgt tagtaaacat tatcgttttt agtgaaaaag
      181 tatatacgtt aattattatt aaatgtgaga ggataacaaa aaaatgaaag acctattacg
      241 aaatagtcta gagcaaagtg gaaatttaag ttttcaagat atgattttac atattcttgt
      301 agcagcttta ttgagtgtag ttatttatgt ttcctatgct tatacgcata gtggaactgc
      361 ctatagtaaa aagtttaatg tttcattaat gacattgacg gtcttgactg caacagtaat
      421 gaccgttatt ggtaataatg tagccttgtc attgggtatg gtcggtgcct tgtcagttgt
      481 tcgttttagg acagccataa aagattcaag agatacagtt tatatttttt ggaccatagt
      541 tgttggtatc tgttgtggtg tcggtgacta tgtggtagct gcattaggaa gtagcgttat
      601 ctttatctta ttatgggtta tgggacgtgt taaaaacgag aatcgtatgt tattgattgt
      661 gaagtgcgat agaacactag aagttgattt agaaggaatt ttcttccaat attttgacgg
      721 aaaagctgtt cagcgtgtta aaaattcaac aactaatact attgaaatga ttttcgaaat
      781 ctctagaaaa gattacgata agcaactcca tgtagataat cagttaactg aaaaagtgta
      841 ccaattggga aatattgatt atttcaacat tgttagccaa agcgacgaaa tcaatgggta
      901 gacttatgag agggaaggtt atttacggca caacccttat aggtcttttt ctattcttat
      961 ttttctattt ttggattcct aagcatcaca tcgagagaat acatcatcat cgtataaagc
     1021 aggtagatgc gaagagtgat ttaacaggat ttaaaaccca tttgcccatt atcagcattg
     1081 atacaaagca acaagttatt cctcttgtta caaaagaagg cggaaaatat gtcaaagcta
     1141 gggataatat taatgttgat atcgaattac gggattctcc aagtagatca catcatttat
     1201 cagaaaagcc gagaattagg acaaaagggt taatatcata tagaggaaat tcctctcgtt
     1261 actttgataa gaagtcattg aaagttaagt ttgttactaa taagttaaag gaaaagaagc
     1321 atcgattagc aggaatgcct aaagaatcgg agtgggtatt gcatggtccc tttctagaca
     1381 gaacattatt aagaaattat ctgagttata atattgctgg tgagattatg tcctatgccc
     1441 caaacgttcg ctactgtgag ttatttgtca atggtgagta tcagggagta tatctagcag
     1501 ttgagaatat cgaacaaggt gagcaacgag ttcccattga aaaaagtgat aaaaagttgc
     1561 ataaaacacc ttatatcgtt gcttgggatc gtgaacataa ggcaaagcaa aagttagata
     1621 actatgtaca ttatacacac cagtctggta tctcagctct tgatgtgaaa tatccaggta
     1681 aacaaagatt gacatcaaaa caattagagt ttattaataa ggacatcaat catattgaaa
     1741 aagtccttta ttcttatgat ttttcacaat atcctaaata tattgataga gaatcatttg
     1801 caaattattt tgtcatcaat gaatttttta gaaatgtgga tgcgggtaaa ttttcaacct
     1861 atctatataa agatcttcgt gatagagcaa aactagtagt ttgggatttt aataatgctt
     1921 ttgataatca aattgaagga cgtgttgatg aggcagattt taccttgacg gatgcaccgt
     1981 ggttcaatat gctcattaag gacaaagcat ttatagacct ggttgttcat cgctataaag
     2041 aattacgaaa gggagttcta gcgacagaat atttatctaa ttacatagac gagacaaggc
     2101 attttttagg tccagctatt gatcgaaatt ataaaaaatg gggctatgtc tttgatttaa
     2161 agaatactga tccaaggaat tatttgattc ctactgaacg caatgtgaca agttatcaca
     2221 aatcagtaga acaattaaaa gattttatca aaaagcgtgg cagatggatg gatagaaata
     2281 ttgagacctt gtatcaatat agtgcaccat cgaaaaatac taatacttta ctggagtaga
     2341 acattatgaa aaaatatcta agtattgtag cccttttaac tattttgttt agctctattt
     2401 attatttgta ttactttgat ggtagtttgt atttaccaaa gggcttatta aaagaaaata
     2461 caagaactaa ctttgttgtt aaaggtgata ctgtacttca caagcccacc aataaacctt
     2521 ttgttgttaa aggagtagac gttgagtctt ccttagcggg ttatcatcac aacgattttc
     2581 ctattactca aaaaacgtat cgtgaatggt tccatttaat ttccaacatg ggggcaaata
     2641 ctgtaagagt caaggtaccg atgaatgttg cattttacga tgccttatat caccacaaca
     2701 aagcatcaaa gaggccactg tatttgttgc aaggaatacg tatagattct tatcgcaata
     2761 atgcttctat aacagctttt aatgataatt atagggggta tttaaaacga gaagcaaaag
     2821 gcgttgtgga tattctccat gggcgtaagc aagtatggaa tactgatttg ggtagccgtc
     2881 attatcatta tgatcttagt ccttgggtac ttggttatgt cgtaggggat gattggaata
     2941 gtggtactgt cgcttatact aatcatcaag agaaaaaaac gcaatataaa ggacgttatt
     3001 ttaaaacttc tgtggcagct aatccatttg aggtcatgct agctcaagta atggatgaat
     3061 tgacacatta tgagacagct aaatatggtt ggcaacattt gattagtttt tcaaactcac
     3121 caacaacaga cccttttcat tatcgaaaac catttgaggc acaggctcct aaatacgtac
     3181 aactaaatgt agaaaatatt caagctaatt caaatgttaa agcaggtatg tttgcagcat
     3241 ataaagctat tgatttccat cctcgataca aggattatct attatttgat aaagagaata
     3301 tcagtaaaga agatagacaa aagattaaag aactttcttt gtcacaggga tacgttaaac
     3361 tgctaaatgc ttatcacaaa atccctgttc tagtcacggg ttatggctat tcgacagcga
     3421 gaggtattgc ccaaaaagaa attgataaac gtcctctgcc gattaatgaa aaagaacaag
     3481 gtcagcgttt actagaagat tatgaatctt ttatatcatc cggtagtttt ggagcgacta
     3541 tcaatgcatg gcaagacgat tggaatgcaa gggcgtggaa tacatctttc gccacaaata
     3601 aacatagtca attcctatgg ggggatgcac aagtatttaa tcaaggttat ggtttattag
     3661 gctttaaaaa cgcaaaacat cattatcaag ttgatggtaa aagaggcaaa ggagagtgga
     3721 aacatcctct gatgactagt gcaacaggag atgacttata tgctagcagt gatgaaagct
     3781 atctctacct tgcgattaaa acaaaacctg aaaaactaaa agaaaaacga ttattaccaa
     3841 tagatattac accaaaatct ggtagtagaa aaatgaatgg tagtaaggtc acattttcta
     3901 aatctagtga ctttgtattg tctattgatc caaatggcaa gtctgaatta tttgtccaag
     3961 agcgctataa tgccttaaaa gcgaactatc ttcgacagct taacggtaaa gatttttatg
     4021 ctttcccacc aaagaagaac agtagtaatt ttgagcagat aaatatggta ttgagaaata
     4081 caaagattgt tgaagacatg gaaaaagtaa aagcaacaga gaggttctta ccaactcatc
     4141 ctactggtct tctcaaaaca ggaacaactg ataggcacca aaaaacattt gattcacaaa
     4201 cagatatttc gtttggaaag gactttatag aggtcagaat tccgtggcag ttgttgaatt
     4261 tttctgatcc atcatctcaa aaaattcacg atgattactt taaacattat ggtgtgaagg
     4321 agttagaaat tgagagcatt gctttaggat taggtgctaa tagcaaagaa aacacactga
     4381 taaagatggc agattatcgt ttgaaaaatt gggagagacc cgataccaaa acctttttaa
     4441 aagactccta ttatagtatt aagaaagaat ggtctaaaga aagagagaga acatatggta
     4501 cataatattt tatattttta cattttgatt tgcttagcaa ttattgtttt taacattcac
     4561 tatatcttaa gacaacgatt agataagctt agttggcttc gtattcggaa aaaggatata
     4621 aatatcaaat aaaggggatt ttctatggca ttatttttta ccttcctaaa ggtatctcaa
     4681 gtcattttct tagtattatt gattgcctat gccagctttt tagtgatttc attagtcatt
     4741 ggaggtgttc atctctatga aaaaaatgag cgcaaaaaaa gtttaactag aaataaagaa
     4801 aaaattccaa tttcaattat tgtaccagcc tataatgaat caacaaccat tgtgagttcg
     4861 attgattcgt tattgcacct tgattatgaa gcgtatgaaa ttatcgttgt ggatgacggt
     4921 tccagtgaca atacttcaga tgttctcaag gaagagtttg cattaatgaa aatctcgaat
     4981 acgattgatt caattattgc tactcaaact tgtaaagatg tctttcagag acaggttggt
     5041 aaggttaaac tgacactcat tgttaaagaa aatggtggca aaggggatgc cttaaatatg
     5101 gggatcaatg ctgctaatta cgattatttt ctatgtctag atgcagattc tatgttgcag
     5161 gtagattctt tgagtcaaat tagtaaatcc atacaggtag atccgacggt tatagcagtg
     5221 ggagggctcg tccaagttgc ccaaggtgtt aagattgagc aggggaaagt agcatcttat
     5281 cgtttgcctt ggagaattat tccttgtgca caagcacttg agtatgattc atcattttta
     5341 ggagctcgaa tctttttaga ctatttaaga gctaatttaa ttatttcggg ggctttcggt
     5401 ctttttaaga aagaccttgt taaagcggtg ggaggatatg acacccagac tctaggagaa
     5461 gatatggagt tggtgatgaa attacatttc ttctgtcgta ataataatat tccttatcgt
     5521 atttgttatg aaacagatgc tgtttgttgg agtcaagcac caacgaattt aggggattta
     5581 cgtaaacaga gacgccgatg gtatcttggc ttgtaccaat gtttaaaaaa atataaaagt
     5641 atttttgcca attatcgctt tggagctgta ggatcaattt cttatattta ctatatttta
     5701 tttgaattac tgaccccttt tatagaatgt tttggtattg tcattatatt tttgtcctta
     5761 ttatttaatc aattaaacat cccattcttt atatcattgg tgagccttta tatcttttac
     5821 tgtgttttaa ttacattaag ctctttctta caccgtattt attctcagca actagtaata
     5881 ggaattttag atattgttaa agtattttat attgctgttt ttaggtatct tatcctacat
     5941 cctgtattaa cttt
//
LOCUS       DATRSD010000091         5874 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRSD010000091 DATRSD010000000
VERSION     DATRSD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.092"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..303
                     /locus_tag="VBD85_001724"
     CDS             <1..303
                     /locus_tag="VBD85_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7134265.1"
                     /translation="EIGIIQSGGAIKKFLADNQVLFNGDLENRRGKKLRLGDIITIPD
                     QNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNKDKSKTSKKPVRFPGT"
     gene            306..1415
                     /gene="recF"
                     /locus_tag="VBD85_001725"
     CDS             306..1415
                     /gene="recF"
                     /locus_tag="VBD85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO7134266.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLSLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLSVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1427..2293)
                     /locus_tag="VBD85_001726"
     CDS             complement(1427..2293)
                     /locus_tag="VBD85_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO7134267.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2349..3011)
                     /locus_tag="VBD85_001727"
     CDS             complement(2349..3011)
                     /locus_tag="VBD85_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134268.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     KQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVKQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(3086..4567)
                     /gene="guaB"
                     /locus_tag="VBD85_001728"
     CDS             complement(3086..4567)
                     /gene="guaB"
                     /locus_tag="VBD85_001728"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO7134269.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(4724..5209)
                     /locus_tag="VBD85_001729"
     CDS             complement(4724..5209)
                     /locus_tag="VBD85_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7134270.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5219..>5874)
                     /locus_tag="VBD85_001730"
     CDS             complement(5219..>5874)
                     /locus_tag="VBD85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7134271.1"
                     /translation="FRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFFEGDYREKLF
                     LIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAITDIEYFSLPM
                     ALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLATLLLQVPTE
                     RGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNKNYFKKFIE"
BASE COUNT         1994 a         1147 c          895 g         1838 t
ORIGIN      
        1 gagattggta tcattcaaag tggcggagcc attaaaaaat ttttagcgga taatcaagta
       61 ttgtttaacg gtgaccttga aaataggcgt ggaaagaaac tccgcctggg ggatattatt
      121 actattcctg atcaaaatat cgagatcatt ataaggaagc catcagatca agaaattgaa
      181 gaacgtaatg tagaaatagc tgaaaaacag cgtgtttcag caatcgttaa agaaatgaat
      241 aaaaacacca acaaagataa atcaaaaact agcaaaaaac cagttcgctt tccagggaca
      301 taaaaatgtg gataaaaaat atttctttaa aacattatcg taattatgaa gaagcacaag
      361 tagatttttc acccaacctc aatattttca ttggccgcaa tgctcaagga aagacaaatt
      421 ttttagaagc aatctacttc ttggctttga ctcgaagtca tcgtacccgt tctgataaag
      481 aacttgtaca ttttaaacac catgatgtac aaattacagg tgaggtaatc cgcaaaagcg
      541 gccatcttag cttagatatt caattatctg aaaaaggaag aatcactaag gttaaccatc
      601 ttaaacaggc aaaattatct gattatattg gggctatgac tgtcgtactt tttgcccctg
      661 aagatcttca attagttaaa ggtgcaccta gcctaagacg gaaattttta gatatagata
      721 ttggacaaat taagccaacg tacttagctg aactttcaaa ttacaatcat gtattaaaac
      781 aaagaaacac ttatctaaaa actactaata acgtcgacaa aactttttta tcagtccttg
      841 atgaacaatt agctgattat ggaagtcgtg tcattgaaca ccgttttgat tttatccaag
      901 ccctaaatga tgaagcggac aaacatcact acattatcag tactgagtta gaacatttat
      961 ctattcacta caaatcgtca atagagttta cagataagtc aagtattagg gaacattttt
     1021 taaaccaatt atctaaaagc cactcaagag acatctttaa gaaaaacaca agcatcggac
     1081 ctcatcgtga tgatattact ttctttatta atgatatcaa tgctactttt ggtagccaag
     1141 ggcaacaaag aagccttatt ttatcattga aattagcaga aattgagcta ataaaaaccg
     1201 ttaccaacga ttatccgatt ctcttacttg acgacgtcat gagtgaactt gacaaccatc
     1261 gtcaactaaa actcttagag ggaatcaaag agaatgttca aacatttatt actacaacaa
     1321 gtttagagca tttaagtgct ttacctgatc aactcaaaat ctttaatgtt agtgatggta
     1381 ctattagtat taatgaaaaa aaagcaacag attaatctgt tgctttttaa taagatttaa
     1441 caataccaag taatatagca cccataacaa aacaaaggat acccatgaca acccatttct
     1501 gttctttctt cgtttttgtc tcacctaaaa ataaaatacc accaataata gagataatta
     1561 ctccaagttg agaaaaacta aaagcaattg ctagccctgc tttagctgct gccaataaca
     1621 tgaagacatt accaacgccc cacatgagac ctgtaatcat atttttaaca acaacagcct
     1681 caaagttaac acgaaacttc atgaaacaaa ttgcacctag acacattcca acagccatgg
     1741 gtaaaataac ggccatagcg tcgaacttca taatgttatt aaataaaact gcgtacgaga
     1801 tgtaacctac agttgaataa gcaattgtag caaatccttt tgaaaactct tgatgtgttg
     1861 ccaaagcttg ttctgagaca tcacgtttac ttgagaaata gaagccgata actaataatg
     1921 tcaacgctgt caatcctaaa ataaattgga ttggcttagt ccattcatga aagactaaag
     1981 caccaactag gcttccacct actaattgtg caccacttga cagtggatta gcaacagaga
     2041 ctcccatata tttcattgct tgaaattgac cattttggcc gactgaccat aggataccac
     2101 ctaagatacc aaaaatccac aatgaggcag tcatctctgg ctgtttaaat aaccatacga
     2161 taatcgcaaa tagcaatgct cctaaagtca tcccaaatgt ttgttgattt ggacgccctc
     2221 caattttatt actaacaaat ccaatacttc cccacgcaaa catgggaatc aatgcaataa
     2281 gtaaaccttc caattctttc tccttacaat ggaatattaa gtattaccta ttttcaatcc
     2341 tagagaatct attgactaac tttattagca agatcagcaa atactatagc tttatcaaca
     2401 ctatcttttt ccaaagctat aatgacttgt tttatcaata aattgaccag tgatttagaa
     2461 taaggtaact gttctttagt catattacta atcttattta aaataacatc ttcaggtaaa
     2521 atatcttgaa aatagcagat gactttgaaa taaaaatcat tgtactgttt cacaccattc
     2581 agataagtaa ctaccaggtt aatctcatct tgaggcagcc tcatatttaa ccctaaattt
     2641 gaaataaaaa gtttagccaa tattttgaga acattgcttt ctattctatc acaggattgt
     2701 tgtttttgga aactgaaaat tctcatgata tcttcattat ctcgataaga ttctagtaaa
     2761 gttagctgct ctaaatcagt attagaagtt ccagccttaa cttttgaatc tagaatggat
     2821 acttcattga tagttacccc aacctccaac gtttgtaaag ctttaataaa tttatcaata
     2881 cctatttgtg tctcaccatt ttcaaaacgt gatagagttg attttgataa ttgagcactt
     2941 ttggctagag aacttaggga tatacctttg tcttctctca actcttttaa aatttttcca
     3001 taatttttca tacaaaaact ttactatatt tgtttctttt atgcaacaaa aagcatcctc
     3061 ataggggatg ctttttgtcc ttattttagt gtactgaata atttggtgcc tcatttgtaa
     3121 tttgaacatc atggggatgg ctttctttca aaccagctcc tgacatttca acaaattgag
     3181 cattatcatg aagttctttt atgttcgctg cacctacata tcccatacca gaacgaatgc
     3241 caccaagcat ttggaaaaca atatcggcta ctgaaccttt gtatgctaca cgaccttcaa
     3301 taccttctgg aaccaatttg ttagcctcgt ttacagaacc ttggaaataa cgatcgcttg
     3361 aacctttttt cattgctgca attgagccca taccacggta tgtcttgaac ttacgccctt
     3421 ggaaaatttc tgtttctcct ggcgcttcat ctgttcctgc aaacattgaa cctagcataa
     3481 ctgcatttcc acctgctgca agtgctttta caatatcacc tgaatattta ataccaccat
     3541 cagcaatgat agtcttaccg tactcacgcg caacagccgc tgcatcgtaa atagctgtaa
     3601 tctgaggaac acctacccca gcaacgacac gagtcgtaca aattgaacct ggaccaatac
     3661 caactttaac gacatccaca cctgcatcat aaagtgcacg cgctccttct gctgtagcaa
     3721 tatttcccgc aatcaatgtg cgattaggga agtgagcacg aatttcagca attttacgga
     3781 gcacaccagc tgaatgtcca tgtgctgtat caatgacaat agcatctgca cctgcttcaa
     3841 acaaagcttc tgcacgttca aaagtatcag aggttacacc tactgcacct gcaactaaga
     3901 gacgtccaaa ttcatctttt gctgccttag gaaattcaat aactttttcg atatccttaa
     3961 tagtaataag tcctgaaaga cgtccctcat catctactaa aggtagcttt tcaatacgat
     4021 gctcatgaag aatacgctcc gcagtttcaa gatctgtacc aataggagct gttacaagat
     4081 tttgacttgt catgtgctct gagattagtt gtttataatc agaaatgaaa cgcatatcac
     4141 ggttagtaat aattccaact aatttacgat tctcgagtgt ctctacaata ggaactccac
     4201 taatgcgata gttttgcatc aattcctcag cttcagagac agtattatct ggtgtcaaga
     4261 agaatgggtc aataataaca ccattttctg agcgtttaac tttacgaact tcttctgctt
     4321 gatcaactat tgacatattt ttatgaatga taccaagccc accagcacgc gcaatagcaa
     4381 tagccatctt actatctgta actgtatcca ttgcagctgt aatgattgga atgtttaatg
     4441 tcaaattatc agccaacttt gtcttcatat caacttcatt tggtaaaaca tggctttcag
     4501 caggaataag cagaacatca tcaaaagtaa aacctttttt caaaaactta gtatcccaat
     4561 ttgacatcaa aaaatcctct tttcttagat agtagatata aaaaatccca ttaaaattat
     4621 attgttacta tcatatcacg ctaaaatcat ttgtcaatat aaaaagagca tagaattaat
     4681 agcttctagc tctttaaagt aatgaaaaaa tcttcttaat tttctatttt tttgagaaga
     4741 agaaaggcgg cgtatactga cttaatttct caaaacactt ttgagcttct tcactattat
     4801 tacaaataat caaacaagta tcatcaccac agacagttgc taaaatctca ggtaataaaa
     4861 tcgaatctaa tatggagcca aaagattgag ctaggccagg tagtgtcttc aaaacgattt
     4921 gattttgaac tggaaacagc atgactaggg catcttccat gtaaaaacgt aaccgttctt
     4981 cccaacgctt ctgagatata ctatgaatct cataataagt ttctgtagca ttatcactgc
     5041 tttcgttaac cttaattaaa ttaagttctt tcatatctcg agataatgtt gcttgcgtca
     5101 ctgaaacgcc atttttttct agtaattccc ttaactcatg ttgtgtacgc accttagtct
     5161 cagagactaa ggaacgaatt aattggtgtc ttgtttcttt tttattcata taaactcctt
     5221 attcaatgaa tttcttgaaa taatttttat tattatagag aagtttcttt cctttcattt
     5281 caacgatatc tttttggcgt aattccttta aaacatgact aactgtctcg cgagtagttc
     5341 cgctcatatt ggcaatttca atcgttgtaa taggaaaagg taaatgtccc ctttctgtag
     5401 gaacttgcaa aagtagagtt gcaagcgatt ggataactct catactggca cttgaagtca
     5461 ccatattacg aagtcgtatt tcatgaactc gtaatagctt agaatatttc cgacaaagat
     5521 gcttcatttg attgatattt tgaagagagt attcttcgta taaagccatt ggcagagaaa
     5581 aatactcaat atccgtaata gctactgctg aaaaatggta atcatcatcc aaaaagagac
     5641 ctccataagg aaaaactgta tcttgtctaa cataatctgt ataaataaaa gaaccagaag
     5701 cgtctgattg ttcaattttt acatgtcctg actggattaa aaacagtttt tcacggtaat
     5761 caccttcaaa aaataaggta tgatttttta gagctgtacg atgtttaatg tggctcacaa
     5821 tatggtcaaa ttgttcaata gtaaagtgtt taaaatcatc tagttgtctg aaat
//
LOCUS       DATRSD010000092         5709 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRSD010000092 DATRSD010000000
VERSION     DATRSD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.093"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            116..1261
                     /locus_tag="VBD85_001731"
     CDS             116..1261
                     /locus_tag="VBD85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7134272.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKLGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1261..1896
                     /locus_tag="VBD85_001732"
     CDS             1261..1896
                     /locus_tag="VBD85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134273.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKIPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQSKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1899..2825
                     /locus_tag="VBD85_001733"
     CDS             1899..2825
                     /locus_tag="VBD85_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7134274.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKSGHTTIKVAAQSST
                     ESSIMANIITELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2826..3476
                     /locus_tag="VBD85_001734"
     CDS             2826..3476
                     /locus_tag="VBD85_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134275.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISVYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3538..4719)
                     /locus_tag="VBD85_001735"
     CDS             complement(3538..4719)
                     /locus_tag="VBD85_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7134276.1"
                     /translation="MNINGIKLLSSRAVSKLGDVFYDYGNSTWIASMGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWIPYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4792..5652)
                     /locus_tag="VBD85_001736"
     CDS             complement(4792..5652)
                     /locus_tag="VBD85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
                     /protein_id="HEO7134277.1"
                     /translation="MFKYVPIFREFRLNRQFSLKQVASNELSVSQLSRFERGESDLSL
                     TKFLGALEAIDLSISEFMDRVNKYQKSDQISLMSQMAQYHYQRDVAGLEKMISVEEGK
                     LKKDSSDIRCRLNIVLFRGMICECDSSRKMSEEDLCFLSDYLFQKDSWEISDYILIGN
                     LYRYYNTRHICQLVKEVINQKEYYRDIYTNRNVVEATLLNVVETLIERRALEEATFFL
                     EKVEALLNNERNAYHRIILLYEKGFLAYAKGDSRGIQSMKQAIFCFQAIGSKHHVENF
                     QEHFNRVTRL"
BASE COUNT         1931 a         1015 c          929 g         1834 t
ORIGIN      
        1 atcaggctca agcttaacga ttctttttaa aatcattaaa ttttaaaaca aatttcagac
       61 atgttgccaa agttttgata ttattactat aatatagttt gtagaggaga ataatatggg
      121 ccaagaacct atcatcgaat atcaaaatat caataaagtg tatggggaaa atgttgcggt
      181 tgaagacatt aaccttaaaa tttaccctgg tgattttgtt tgtttcatcg gtacgagtgg
      241 atcaggtaaa acaacattaa tgcgtatggt taaccatatg ttaaaaccaa caaatggtac
      301 tctattattt aagggaaaag atatttctac tattaacccc attgaattaa gacgcagaat
      361 tggatatgtt atccaaaaca ttggtttaat gcctcatatg accatttacg aaaatatagt
      421 tcttgtacca aaattattga aatggtcaga agaagctaaa agagctaaag caagggaact
      481 tattaaatta gttgaattac ccgaagaata tttggatcgc taccctagtg agttgtctgg
      541 cggtcagcaa caacgtatcg gtgtcattcg cgctcttgca gcagaccaag atattatttt
      601 aatggatgag ccttttggag ctctggatcc tattactaga gaaggtattc aagacttagt
      661 caagtctctt caggaagaaa tggggaaaac tatcatctta gttactcatg atatggatga
      721 agccctcaag ttagcaacaa aaattattgt tatggacaat ggtaaaatgg tccaagaagg
      781 gacacccaat gatctcttac atcatcctgc tactagtttc gttgaacaaa tgattggaga
      841 agagcgtctc cttcatgcgc aggctgatat tactcctgtt aaacagataa tgttaaataa
      901 tcctgtttca ataactgctg aaaaaacact aactgaagct attacactaa tgcgccaaaa
      961 acgcgttgac tcacttctag taaccgataa cggtaaatta attggtttta ttgacttaga
     1021 atctctaagc agtaaatata agaaagatcg acttgtttct gatatcttaa aacatactga
     1081 tttttatgtt atggaagacg acttacttag aaatactgct gagcgtattt taaaacttgg
     1141 tttaaaatac gctccagttg ttgaccatga gaataactta aagggcattg ttactcgtgc
     1201 atccctagtt gatatgttat acgatattat ttggggcgat actgaaacgg aggatcaata
     1261 atggtcaatt tcttatcaca gtatggcatg caaatattag taaaaacatg ggaacaagtc
     1321 tatatctcat tctttgctat agcacttggg attgcaattg ctgttccatt gggtgtggtc
     1381 ttaacacgtt ttcccaaagt agccaaaatt atcattgcta tagctagcat gttacaaact
     1441 ataccaagtt tagccttatt agctttaatg attccattgt ttggaattgg aaaaatccca
     1501 gctatcgttg ctttatttat ttattccttg cttcctatac taaggaatac ttatataggt
     1561 atgaataatg tcaatccaac acttaaagat tgtgctaaag ggatgggaat gaaacctatt
     1621 caatctatat ttcaagttga gttaccttta gctactccaa ttattatggc tggtattcgc
     1681 ttgtctacta tttatgttat tgcttgggct acattggctt cttatatagg tgcaggtgga
     1741 cttggtgatc tcatttttag cgggttaaat ctttttcagt ccaaattaat tctagggggc
     1801 acaattcctg ttattatcct gtcacttatt atagattatc ttttaggatt actggaaaca
     1861 gctctaacgc ccagaacaac aagaagggag gcttagatat gcttaaaaaa tcgcactttt
     1921 tacagatatt tacactttgc ttagccctct taacgatttc tggttgtcaa ttaaccgata
     1981 ctaaaaagtc tggtcatacc acaattaagg ttgctgccca aagttctaca gagtctagta
     2041 ttatggcaaa tattatcacc gaattaattc atcacgaatt aggatacaac acaactttaa
     2101 taagcaatct tggttcctct acggttactc accaagcttt gctccgtggt gatgctgaca
     2161 ttgctgccac acgttataca ggaacagaca tcacagggac tcttggttta aaagctgtta
     2221 aagaccctaa agaagcttct aagattgtaa aaactgaatt ccaaaaacgc tacaatcaaa
     2281 cttggtatcc tacttatggt ttttctgata cttatgcatt catggttact aaagagtttg
     2341 ccagacagaa taaaatcact aagatctctg atcttaaaaa attatcaaca actatgaagg
     2401 caggggttga tagttcatgg atgaatcgcg agggagatgg atacactgat ttcgctaaaa
     2461 catacggttt tgaattttca catatttacc ctatgcaaat tggcttagtc tatgatgcgg
     2521 ttgaaagtaa caaaatgcaa tctgtattag gctactccac tgacggtcgt atttcgagct
     2581 atgatttaga aattttaagg gatgataaaa aattctttcc tccttatgaa gcctctatgg
     2641 ttgtcaacaa ttctatcatc aaaaaagatc ctaaactaaa aaaattactc catcgactcg
     2701 atggtaaaat caatttaaaa acgatgcaaa accttaatta tatggtagat gataaacttt
     2761 tagaaccttc agttgttgcc aaacaatttt tagaaaaaaa ccattatttt agaggagata
     2821 aataaatgaa acaaatgaat actttccaac aatttatcta ctacttccaa cataatggta
     2881 gttatatctt agaacaattt atccaccact ttttaatttc agtttacggc gttattttag
     2941 ctgcaattgt tggtatccct ctaggtatct tcatcgctag acgtggtaaa attaaaacct
     3001 ttgttatggg aatcgcaaat gtcatccaaa ccattccttc tttagctatg atctctatca
     3061 ttatgttagg tctagggcta ggtattaaaa cagtagttgc aactgtcttt ctttattctc
     3121 ttctgccaat catcacaaat acttacactg gtattagaaa tgttgactcc gacttactag
     3181 atgctgctaa aggaatgggc atgactaagc gtcaacgttt atttatggtt gagttaccac
     3241 tatctatttc cgttatcatg gctggactta gaaatgccct tgttgtagca attggtatta
     3301 cagccattgg tgctttcgtc gggggaggcg gacttggaga tatcatcata agaggaacta
     3361 atgcaacaaa tggtggtgct attatcttag caggatctct tccaacagcc cttatggcaa
     3421 ttttctctga tttaatccta ggcggtatcc aacgcatgct tgaaccaaga aaataactat
     3481 taaaaacttg tcagtagaga caagtttttt tgatgtttca gaataatttc ttaagttcta
     3541 aaattgtttt ttactatagc taaatcctaa tcctcccaat atagcaatgg cacatcctat
     3601 aacaataaag ctaacatttg ataaggcaaa tgaaaagata gtcataaata gtgttcctat
     3661 aggcataaac ataatagcaa tagtaaatat agtcgacatc acacgcccca tatacgcttc
     3721 atcgaccctg atttgtacct gactgaaaaa atgaatatta aacattgtca gaagactatt
     3781 aaaaagaaaa ctaccactgt aaggtatcca tatcggtaat tcaaatagag atgggaatcc
     3841 cataactatg actcccaaac tactgaatac tagcatagat agcatagaat taatactaga
     3901 tttaattttt ctagcaataa gcgctccaat aattgatccg atagctgata tactaagtat
     3961 tgttgcataa gcacctgaag tttttaatag ggagttagta aaaggtaaaa gataattgaa
     4021 cattgctagg aaagtgttta ataaagcagc aataattaga aaaaacataa tttctttttc
     4081 ttttttgatg taagtaaatc catctgcaat atcagctaaa atatttttta tcgctacctt
     4141 ttcttgtttt gaaagctgta ccctttccac tttaatagca taaagaaaca ggaaagagat
     4201 caaaaaagta atcgcatcaa caattaaagt aatcctaata ccaaaattat taaaaataag
     4261 gaaacctaaa acaggggaac ttacactaat aatttgcaca atggtttcca gattagcatt
     4321 ataggtaatg atatcagctt tatctactat ttcaggaata taagatttat aagccggaga
     4381 agaaaaagca ttggaaaccg ctaagatagc attagcaact attagaccat acaccattac
     4441 tttatcatct ccaataaaag aaagtaagaa acacatgata gcacagatag catctgttat
     4501 tagtaaaatt tttcttcgtt gaaagcgatc agctaaggcg cctccaaaag ggttgaggac
     4561 aatagaaacc aacaactcta ctatctgata aatacctaaa atcttttgtc ctagaccacc
     4621 catcgaagca atccaagtac tatttccata atcataaaac acatcaccaa gtttactgac
     4681 tgctcgacta gatagtaatt ttataccatt aatattcata attccctcct tagttcgaca
     4741 ttatagcaaa ccaaaaggag aaatattaaa aaatcccata aatgggattt tttataatct
     4801 tgttacccta ttaaagtgtt cctgaaaatt ttcaacatga tgtttagaac caattgcttg
     4861 gaagcaaaaa atagcttgct tcatgctttg tattcctcga ctatcccctt tagcataggc
     4921 aagaaatcct ttttcataga gtaaaataat acggtgataa gcattccgct cattatttaa
     4981 aagtgcttca actttctcta gaaaaaacgt tgcttcttct agagcgcgcc gttctattag
     5041 tgtctctacc acatttagga gggttgcttc gacaacattg cggtttgtat aaatatctcg
     5101 ataatattcc ttttgattga taacctcttt aacaagttgg caaatatgcc ttgtgttgta
     5161 ataccggtaa agatttccaa tcaaaatata gtcactaatt tcccaactat ccttttgaaa
     5221 gaggtaatcc gacaaaaagc aaaggtcttc ttccgacatt tttcttgagg agtcacattc
     5281 acatatcatt cctctaaaaa gaactatgtt taaacgacaa cgtatatctg aactatcctt
     5341 ttttagttta ccttcttcca cagaaatcat cttttctaac ccagctacgt ctctttgata
     5401 atgatattga gccatctgag acattaaaga tatctgatca gatttttgat atttatttac
     5461 tctatccata aattcgctaa ttgataaatc aattgcctct aaagccccta aaaattttgt
     5521 taatgataaa tctgattcac ccctctcaaa tcgtgataac tgagaaacag aaagttcatt
     5581 tgaagcaacc tgtttaagag aaaactgtcg attaagtcga aattccctaa aaatagggac
     5641 atatttaaac attagtattc ctttctaacc gtaatctcat tggtaaactt atgaggtaat
     5701 ttcaataat
//
LOCUS       DATRSD010000093         5683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRSD010000093 DATRSD010000000
VERSION     DATRSD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.094"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..369
                     /locus_tag="VBD85_001737"
     CDS             <1..369
                     /locus_tag="VBD85_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7134278.1"
                     /translation="LSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDE
                     ILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWN
                     YHRIHGSLNYQTPMTKRLIA"
     gene            593..1725
                     /locus_tag="VBD85_001738"
                     /pseudo
     CDS             593..1725
                     /locus_tag="VBD85_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1765..2004
                     /locus_tag="VBD85_001739"
                     /pseudo
     CDS             1765..2004
                     /locus_tag="VBD85_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2203..4671)
                     /locus_tag="VBD85_001740"
     CDS             complement(2203..4671)
                     /locus_tag="VBD85_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7134279.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATVKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNTGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(4684..5604)
                     /gene="lmb"
                     /locus_tag="VBD85_001741"
     CDS             complement(4684..5604)
                     /gene="lmb"
                     /locus_tag="VBD85_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO7134280.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         1664 a         1137 c         1048 g         1834 t
ORIGIN      
        1 ctgtctcttg gttggcacaa gaccgctgaa ctcgttaagc aagccataca aagcatccct
       61 tacgccctga ccaaagtcaa gatgttccat tcagatcgtg gcaaagagtt tgataatcag
      121 ttaattgatg aaatattgga agcctttgga atcacacgtt cgcttagtca ggctggttgt
      181 ccttatgaca atgccgtagc tgaaagtacg tatcgtgctt tcaaaattga atttgtttat
      241 caagaaacct ttcaattgct ggaagaacta gctcttaaga ctaaagacta tgttcattgg
      301 tggaattacc accgcattca tggtagtctc aactaccaaa caccaatgac taaaagatta
      361 attgcctaga aaaacacttt ataaatgttg tacagaaaag tgttgccttt tcacttaata
      421 aaagaaagat tgaaggaaca cttctctttc attccgacca agggtcacaa tttaagtccg
      481 gggaatttag aaaaataatt gatgacaaca ataggcactg aatccctaaa atgagacaaa
      541 agaaaaacac tcctgttgaa atttagtaaa ctagaaacag gagtgtttta ttatggttaa
      601 aaaagcatat tcatgggaaa ctaagctggc ttgcatcgat atgaagaaag caggcaagtc
      661 taacagagtt attatggaga ctttaggcat caaaaataat agtcaaatct atacttggat
      721 gaaatggtac gaaaacgagg agctttatcg ttttcatcaa ggtgttggca agcaatatac
      781 ctacggtaaa ggcttggaac atctgtctga agtggaacaa ttacaattac aggttgacct
      841 cttaaaaaag tatcgaggct tgataaggaa atcgataaag taagtcttat caagcttgtg
      901 gaagactata aaaacaacta cccgatagtt ttcattctag actgttttaa ggtcaagcgg
      961 tcgacctatt accgttggaa aaaggagtat gagaagccgc aaaaaaagat gaccttattg
     1021 aattaattga acagctctgt atggagaatc actttattta tggctatcgt accattacac
     1081 gattgctcaa aaaaatccac ggattgactg ttaatacaaa gaaagtctat cgtatcatga
     1141 aaaataatgg ctggctttgt cggacacgta ccaagaaagt tcccagtttg ggtaaagctt
     1201 attatttaac agataataaa ttgagtcggg atttccatgc cgataagcct aaggaaaagc
     1261 tcgtaacaga tattacctac ctatatttcg gtaactgtaa attgtatctc tcatcaatta
     1321 tgaatctcta taaccgagaa attatagctt acactatctc agattgtcag gacacggatt
     1381 ttgtactaga cacccttaat cagttaaaat tgcctaaata aaggagcaat cttacacagt
     1441 gaccaaggct cagtgtatac ttctatggct tactaccagg cttgcacgga aaaagacatt
     1501 atccgctcaa tgtctcgaaa aggaacacct gcagataatg cctgtattga atggtttcac
     1561 accgtcctaa agactgaaac cttttatttc cataatagga gaaaatacaa caaagatagt
     1621 ataacaaata ttgttaaaaa ttacattaca ttttataatg aaactagaat tcaacagaga
     1681 ttaaacgacc agtcgcctgt acagtacaga aaactgattg cctaaaagtg tttttcttgc
     1741 gtctcactat tagggtgcag tgccaatatc atgcattctt tttctaatcc tggatatcct
     1801 tatgataatg ccgtaactga agcatttttc aagtatttaa agcatagaca aatcaaccga
     1861 aaacattatc aaaatatcaa acaggttcaa ttagactgct ttgaatacat tgagaatttt
     1921 tataacaatt acaacccaca tacggctaat ctaggactaa cccctaatca gaaagaagaa
     1981 aattatttta acgcaataaa ataacacagt tttctgtcta cttatttgac attagtccaa
     2041 gaatcctcct ctttttttac tattctaaca tggataccat ttctttgcaa aagaaaaccg
     2101 ctattatcca acttttatca ctactcgtta catagaatga cttcaaaatc accgtaactt
     2161 gctttgacaa agcagtgctt taacaatgag atcttctttt ggttaagggt ttatttgttg
     2221 aagtgtcttg atatcataag ttaccaattc accattttta ttataaaatt ggacaccttc
     2281 tggttggaaa atgagatact taggatcgat attagctttt tgtgctaatt gattgatatg
     2341 atcttctagg gttgctctat ctagaccata gtctggtaaa ctgtctataa agtcatctga
     2401 ttctttttct tctttactgg cttcacttgg ctgttggttt tcattgctct tatctgcctg
     2461 ttgcttttct ttgggtttat ccgtatcagt agcatcacca gtatttttct ttgccaataa
     2521 ctcttgagct tgttgccact cagcttggga tagatcagat ttattaatgg ttctcaatga
     2581 actgccatct gctcttggga tgctaaagga gccatctttc catacaaaag tttctttggc
     2641 caaaacatct cgtggatcga aaatatagcc gtctggtgtt gcaaaacgac cttctgctag
     2701 ggctttttga acttcttcag cagaatggat aatttgccag tttggcatac cagcacgttt
     2761 atcaagaggc gtaacatttg gaatgaccga acctgactct tcatgccctg gcttagacca
     2821 tacatccgga cgaacttcgg ggtgttgcat cacatacttg actgttgcaa tctgatcgcg
     2881 cgtcaaccat gaatacggaa cgacatggat atgatcaata tgtgggataa caaacgaact
     2941 tccagtatcg taagtagcat taccagcatg catcggcaga cttgacacat ctacagcaag
     3001 tcgtggctca atacctgtgt caacaatgtc ataacggtaa tgcttcttat ctttaagcat
     3061 tagttcttgt tcggcaaagg caatctgagt caaatcaagt tgatcacgag catagaaata
     3121 gtccttacca tcttttggca tcatatagcc cactttacca tcttttgtta ctttgcgact
     3181 cactttttta gtgtcaaaga gtggtttttc tttgccttgt tcctgatcca aagcagcagc
     3241 aagctcatca gcttgacctt ttgctttcac ccagttagcg acctcatcca actcatattg
     3301 ttcaagttct ccaaatccta tatagtggaa atgatctccg tgtttagctg taactcctga
     3361 tttatccact gaatgaatgg attctttact aaaaacataa gcatcactcg tatcatatgg
     3421 tttaccatct aagccttttc cgtaagcttt gatgcgatga ccaagaaagg tatgtgtcac
     3481 ttctttatct gatggttttg agtgctctga acctgagtca ttgtcctcag tttggccagc
     3541 taagtatcga tctgctaatt ccatttcaag aggtgataac tgacttcttg ggataatatg
     3601 ataatgatct ccatgaggca ccacataccc aaaagcgttt gatttgatca cttgagtcgg
     3661 ttcaaaaatc aacccatctt cttccacatg acggtatttc aaatcaagac ggtgtagttg
     3721 atctaaaagt tccttaaagg tttttccttt aaactcatct ctttggtgtt tgttttgtga
     3781 cgcatcattt ggcctaggag gcgctggatg atagccaccg ttatctggct gatgaccttg
     3841 tccagggtta ggcgtcacat caggaattgg ggctttccta cgacctgggg ctggtgtcgg
     3901 gcggtaatca gacggtctag cacctcgacc ttgtttttga ctccagtagg cttgtgcagc
     3961 agctagctca cttggagaca aatccttttt aggaatataa tgatagtgat taccatgagg
     4021 tactaaataa gcatctccta aatcatcaat gatatctgtc ggactaaaaa tatagccatc
     4081 gtctgtagta tagcgtcctt gtctttttgc ttcattgact gccgcaactt cttctttact
     4141 gagatgggcc acttgagcta aacctttttc tttagcttct ttagttcctt tggctacttg
     4201 ctcagcaatt tgttgtttgg ttcgaatgtt tttgcgctta ctacctggct tgaggtaaac
     4261 ataatagttg ccattgactt taataacgta accgtctaag atttcattga taacgtctga
     4321 ttgtttaaaa cggtaattag gatccgtcat caacaactct tcactaataa tcgcatcata
     4381 aggaactttc ccattgtaaa aatgataatg gtcaccgtgt gaggtcacat agccttggtc
     4441 agtaattttg actacgatct gttcagcaga gatgccttct tcagcactga tttgatccat
     4501 cgttttgttt gttttagggg cttttgcctt acctttgctg tcatcaatat aggcaatctg
     4561 attgtccttt gttgctagac ccatatgatg cttaccaagt tggtaacttc caatatgagt
     4621 agctagtaaa atggcagcaa ctgagccgat ataaccatat gttttcttca ctaataatct
     4681 cctttacttc aactgttgat agagcacttc caaatttgct ctaagatttt ctagatatgt
     4741 cttgtttccg cttggagcag cttcaagtgg acttaatgtc tttactttag ctcctgttga
     4801 tttcgcaata gcatgagcaa ttttgggatt gacgttgtct tctgcaaaaa tagtcttgac
     4861 gttgtattct ttgacaaagt cttgaatttc tttcaattgg cgaggagagg gctcttgctc
     4921 tggagaaata cccgagatac caagttgttt caagccgaat cgtttagcca gataagaaaa
     4981 tgccgtgtgt tgcgtcacga atgtttttga gcgcaccttt ttaaattttt gagtgtattc
     5041 ttcagttagt tgctctgctt cttttttgaa agccttagcc tttttagtgt aactgtcttt
     5101 gtgtttagga tccaaacgtc ctagctcttt agcgatatta acagcttcct caccagctaa
     5161 aacgggatcc gtccaggtat gtgggtcata aagtgtcgca gggtcaatgc cttgtgtgac
     5221 ttccatatct tctagccctt tgactctatc tagtgtcaaa ggttttgacg cttcaaacac
     5281 atcaaccttt gattttttta aattagggtc tagatccctt gcccaagctt ctaaggtatg
     5341 tgaatggtaa acaaacaaat ccgcgtcata aatagctgcc acatcattta cagacggttc
     5401 aaaggaatga atgcctgcac ctgattggat catcctcaca tcattgaggt ctccagatac
     5461 ttcttttgtc atcgcataca ttgggtaaaa gctggttaca actgacatgc cttgcgtagg
     5521 ctgtttgggg tttgctgact tatcacaccc tgctatcatt actaaactca caaccatagc
     5581 catgagaaaa aaaccttttt tcatagtacc tcctcaatta taatttaacc agttattaac
     5641 cagttaattt tactccttta tcaatgcttt gtcaatggca act
//
LOCUS       DATRSD010000094         5647 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRSD010000094 DATRSD010000000
VERSION     DATRSD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.095"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            31..603
                     /locus_tag="VBD85_001742"
     CDS             31..603
                     /locus_tag="VBD85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO7134281.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSNDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            879..1730
                     /gene="ylqF"
                     /locus_tag="VBD85_001743"
     CDS             879..1730
                     /gene="ylqF"
                     /locus_tag="VBD85_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO7134282.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDEAPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1717..2478
                     /locus_tag="VBD85_001744"
     CDS             1717..2478
                     /locus_tag="VBD85_001744"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO7134283.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSDLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGYAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2499..3047
                     /locus_tag="VBD85_001745"
     CDS             2499..3047
                     /locus_tag="VBD85_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO7134284.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
     gene            3286..4248
                     /locus_tag="VBD85_001746"
     CDS             3286..4248
                     /locus_tag="VBD85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7134285.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLDQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAVTVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISITKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            4245..5219
                     /locus_tag="VBD85_001747"
     CDS             4245..5219
                     /locus_tag="VBD85_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7134286.1"
                     /translation="MIKDLEIPKLLILLILLITAIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFAINWPFIKELDVLLLGKENAI
                     SLGIPYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            5216..>5647
                     /locus_tag="VBD85_001748"
     CDS             5216..>5647
                     /locus_tag="VBD85_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7134287.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVRDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRA"
BASE COUNT         1912 a          933 c          970 g         1832 t
ORIGIN      
        1 cataacagag caaaagagag aggatagtat atgagaaaac gttcaatttt gccaatatta
       61 gctgtcttat ttgttttcat gagtatcttt agcgtatatt ctatttttca tggtgataaa
      121 gccaaagaaa aagcaagtgt cataaaacaa gcatcccaaa caagtcaaac ttcaaaaaaa
      181 gaggtcttac aaaagaagac ttatccaaat ctaaataaat attcaaatct agaaattcat
      241 gtttcatcta cgaggcaaac aatgacaata acctcgaatg acaaggtcat ctttaaaacg
      301 attgtttcaa caggagcaaa agagagccct accccaaaag gaacttttgt catagaaccg
      361 gaacgaggag attttttcta taacgcctca tcaaaagaag gagcctatta ttgggtctcc
      421 tttaaagagc atggtattta tctctttcat agcgttccga ctgatcaaca aggaaatgag
      481 atcccagaag aagcaaagca attaggaaaa gccgcctcac atggctgtgt gcgtatgtca
      541 agggcggatg caaaatggtt ctatgaaaat attcctcaag gaacgactgt taccatcaaa
      601 tagtcttaat ggggtaaaaa tcatgttatt aaaagctcgg agtaaacttc cagagctttt
      661 tcgtatatcc cttaaactca attagtaaac gaaacctatc agcggaataa atgtataaag
      721 tatttcgata tcattaaaaa aatgatagaa taatagtgtt aatcgaatat tttatcgacc
      781 tttatatgcg gttgaagaag aataaaacta aaagattaga cctctaacaa atagatgtag
      841 acttttaagg tagtctatac ataaggagaa tttatttcat ggctactatt caatggttcc
      901 caggacacat gtccaaagct cgccgtcaag ttcaagaaaa tattaagcat gttgattttg
      961 ttacaatttt agtggatgca aggttacctc tttctagtca aaatccaatg ctaactaaaa
     1021 tcgtcggaga caaacccaaa ctaatgatcc tcaataaagc tgatctagca gatcccattc
     1081 gaactaaaga atggagagat ttttatgaat ctcaaggttt aaagacatta gctattaatt
     1141 ctaaagaaca atcaacagtt aaaaaagtaa ccgacatcgc taaaatatta atgtccgata
     1201 aaattgctaa actacgggaa cgtggtattc aaaaagagac acttcgtacg atgattattg
     1261 gaattccgaa tgcaggtaaa tcgactctaa tgaatcgcct agcaggtaaa aaaattgcag
     1321 tggttggtaa taaaccaggt gtcacaaaag gtcagcaatg gctcaaatct aataaggaac
     1381 tggaaatttt ggacactcct ggtatcttgt ggcccaaatt tgaagatgaa ttagttggat
     1441 taaagctagc attaacaggg gcaatcaaag atcaattatt gcctatggat gaggtaacaa
     1501 tatttggatt gaattacttt aaaacctatt atcctgatcg tctcaaagag cgttttaaaa
     1561 gcattaattt agaggatgaa gctcctgaaa tcatcatgac cttaacacaa aaattaggtt
     1621 atcgtgacga ttacgatcgc ttttataatt tatttgtcaa agaagttcgc gatggtaaat
     1681 taggacgcta cacgttagat atcgtcggag aacatgatgg caacaattaa agaaatcaaa
     1741 gcaattttag agactattgt tgatttaaaa gacaaacgtt ggcaagaata tcaaacagat
     1801 tcacgcgcag gtgttcaaaa agcaatcctt caacgaaaaa aaaatattca gtcagattta
     1861 gacgaagaag cgcgcttaga acaaatgttg gtctacgaaa aaaaacttta cattgaacat
     1921 ataaacctta ttgcaggaat cgatgaagta ggtagaggtc ctttagcggg accagtagta
     1981 gctgctgctg ttattcttcc accaaactgt aaaataaaac atctcaatga ttccaaaaaa
     2041 attccaaaga aaaagcatca agaaatctat caaaatattt tagatcaagc gttagcagtt
     2101 ggtattggta ttcaagacag ccaatgtatt gatgatatca acatttatga agctacaaaa
     2161 catgctatga ttgatgcagt ttctcattta tctgtagttc ctgaacatct tttaattgat
     2221 gctatggttt tggatctacc aattccacaa actaaaatta tcaaaggcga tgctaattca
     2281 ctctctattg cagcagcttc aattgtagct aaagtgacca gagataaaat aatgtctgat
     2341 tatgatagca aatatcctgg atatgcattt tctaaaaatg ctggctatgg tactaaagag
     2401 catttagagg gtttgcaaaa atacggtatt actccgatac atcgtaaaag ttttgaacct
     2461 ataaaatcaa tgttgtaaat aatacggaag aggaaaagat gaccgaaaaa gaaaaaatgt
     2521 tagcaggaca atattatcgt ccatcagcac ctgaactacg caaggataga gaagttgcac
     2581 taaaaaatat gcaagccttc aacaatgaag atgatagtag caaacgtaac gtcattttac
     2641 aaaaatggtt tggtgctact gggaaaagta tccatatgga acaacgtttt gtctgcgatt
     2701 atggttgcaa tatttatgtt ggtgaaaact tctacgccaa ttttaaccaa actttcttag
     2761 atgtttgtga aattcgtatt ggcgataact gtatgtttgg cccaaactgt caactcctca
     2821 ccccactcca tcctctagat ccaatagaaa gaaattcagg attagaatac ggtgccccaa
     2881 tccaaatcgg gaataatgtc tggctaggtg gaggagtgac tattttacca ggtgttgtat
     2941 taggagataa tgtcgtcgtt ggtgctggaa gtgtcgttac gaaatcattt gaaaataacg
     3001 ttgttatagc aggaaaccct gctaagataa tcaaaaaatt agcttgaaat tagaaaaacc
     3061 tttaattata acgaaggttt tttctaattt aaaaaatagg taaagtgaat attctgctaa
     3121 tttaatctat cttatttata atgattttaa atagaaaaac cttgatataa taatgttttc
     3181 ttaagtataa aattctttaa aaaagtagat atttttccaa aaaattatgt tattatagat
     3241 ttgtgctcat ttttgaacac tttatctttc attttaagga gttttatgaa aaagctatta
     3301 accactcctg taatgggttg tactttggtt atactttctc taacgtccct atttgttggt
     3361 gttaaatcta tcccactgga gcaaataaca caccttgatc aatcacaggt agatattttt
     3421 ttagcgagtc gcttaccaag aaccattagt atcctaatat ctggtgcaag tttgtcagtc
     3481 tgtggcctgc tgatgcaaca actcacccaa aacaagttcg tttcaccgac aacttcaggg
     3541 actatggatt gggcgaaatt aggagtagtt gttacgctga ttttctttaa aaatacctct
     3601 atttttattc aactatgtat agcttcaggt tttgccattt taggatcgct tctttttgta
     3661 accattttaa aaatgattac ttttaaggat aatattttta tacctcttat cggactgatg
     3721 cttggacaaa ttgtagcagc agtaacagta tttttaggaa cacattttca agtactacaa
     3781 tctgtcaaca gttggcttca aggtaatttt tccatcatga ctagccatcg ctacgaaatt
     3841 ctttacttag cattgccatg tttgttttta gtgtattttt ttgctcatca gtttaccatt
     3901 gttgggcttg gagaaagctt cgctaaaaat ttagggattg cttatgaaaa gatgatttac
     3961 tttggtttag ttttggtttc aattatgaca tccctagtca ttattatcgt aggagcttta
     4021 cctttcttag gtttaatcgt acctaatcta attagcataa ctaaaggaga tcatatgtcc
     4081 tcaactatct tagagacatc gcttttaggg gcttgtatcg tcatgatatg tgatcttttt
     4141 ggtcgcttag ttatttttcc ttatgaagtc tcaataggag ttacgctagg agtattgggt
     4201 agtgcctttt tcttgatttc aattataaga aatgagggaa aatcatgatt aaggatttag
     4261 agataccaaa attacttatt ttactgatac tactaattac tgccattatt ttgtttttaa
     4321 tttatggtat tccaactgac gcaaatgaat ttttgattat ctatatccta aaaacacgtt
     4381 atcaaaaact gattgcatta attttggttg gtatctgcat tggtagttct agtcttattt
     4441 ttcaaactct aaccaataac cgacttttaa ctccgtccat tattggttta gatagcttat
     4501 atgttcttat ccaaacagga ttaatgtatt taatcggagc tcagcgtgtc ataaaatttt
     4561 catcttttag cagcttcctc ctttcattat tattaatggt tggattcgct tacttattat
     4621 ttacgatact ttttcgaaat aaaaaacagt ctttgtattt tgttctatta gctggactca
     4681 tatttaatac attattttct tctatctcat cctttattca agccattatg gaccccaacg
     4741 attttatgat tttacaaaat cagctctttg ctagtttcaa tgctattaat accaagattc
     4801 tttggatttc tttcattatc attgtcgtta gctttgccat taattggcct tttataaagg
     4861 aattggatgt cttactattg ggtaaagaaa atgcgatatc actcggcatc ccatatcaaa
     4921 agcttactac tagaattttt ttatggttag cattgatggt agctatagca acagcattag
     4981 ttgggccgat tactttttta ggcttacttg tagcacatat tacctatcac agttttcata
     5041 cctttagaca tcaaatatta gttcctatag ccattgttat ctgtatcttt acacttgtct
     5101 tagggcagca tctcgttcaa aatctattac acctcactgt tcaactaagt gttctcctca
     5161 accttatcgg gggaagttat tttatattca ccttaatcaa aggaaggaaa aacttatgat
     5221 tcaaataaac aatttacata agttctatgg ccaaaaggaa atattaaaag acattaatat
     5281 ttcgattcct aaaggaaaag taacagctat tttaggtcca aatggatctg gaaagagtac
     5341 attgctgagt tgtatcagtc gacttgaacc ttatgataat ggtgaaattt tccttgataa
     5401 agtaccatta gcccactaca gttcaaatga cttagccaaa acattagcca tattaagaca
     5461 atctaatcac ttaacactca agataaaggt gagagacttg ataggattcg gtagatttcc
     5521 ttatagtaaa ggacggttaa gtcaaaaaga taaagctgtc atagaatcag ttatatctta
     5581 tatggattta aacgatatag ctgacgagtt cattaataac ctcagcggag gtcaaattca
     5641 aagagct
//
LOCUS       DATRSD010000095         5436 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRSD010000095 DATRSD010000000
VERSION     DATRSD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.096"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            37..1410
                     /locus_tag="VBD85_001749"
     CDS             37..1410
                     /locus_tag="VBD85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7134288.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(1568..1873)
                     /locus_tag="VBD85_001750"
     CDS             complement(1568..1873)
                     /locus_tag="VBD85_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134289.1"
                     /translation="MTYELCLEYGTYPLRPVDAWADEINTAPAFITEDKKLLELLEEV
                     NTLFHELFLTIECSFHYIGHDFPEKRAKITQIYHVIIEHLSIHYPEYDIKIESLLMG"
     gene            2036..3022
                     /locus_tag="VBD85_001751"
     CDS             2036..3022
                     /locus_tag="VBD85_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO7134290.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANV"
     gene            complement(3059..4186)
                     /locus_tag="VBD85_001752"
     CDS             complement(3059..4186)
                     /locus_tag="VBD85_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO7134291.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            4290..5309
                     /locus_tag="VBD85_001753"
     CDS             4290..5309
                     /locus_tag="VBD85_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO7134292.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMIVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
BASE COUNT         1615 a          799 c         1110 g         1912 t
ORIGIN      
        1 attaaacaca tctaaaaatc ggaaaggtaa tttgctatgt tgaaaaaact atttgcaccc
       61 cctcagaaaa gagaacgttt atcagctgag gaaattaagc ataaatatcc tcgttatcgt
      121 gtacaagtcc ttatttctat ttttgtaggt tatatgggat actattttgt tcgtaatacg
      181 acttcaattt tatctggtat ccttaatatg tctgctactg agattggtat tattacctgt
      241 gctagctaca ttgcttatgg tctctctaaa tttatttctg gtttgatttc agatgaatcg
      301 aattctaaaa ttttccttcc agttggtttg tttcttacag gactagttaa tgtgttaatc
      361 ggtgttattc catctgtaat tactagtatc tggctcttta ctatcatgta tcttattaat
      421 ggttggttac aagggatggg ataccctcct ggagcgcgta cattagtcta ttggtatgat
      481 aataaagaaa gaattaaata tgctaccatt tggaatttat ctcacaattt tggtggagca
      541 atagcgccga ttttaactgg tgtaggtcta gctcttgctg gtaatgattc tcttaatcaa
      601 gcgcgtgctg cttattggtt tccaggggtg gtggcttgtc ttttggctgt tcttgtttac
      661 tttttacaag aagatactcc agaatctatc ggacttccgc ctatagaaga gtatcataaa
      721 gagcaatata ctaatgttgt ggattcaagt gacattttag aagagcctga agttttgggt
      781 atgggcgaaa ttataaaaaa atatattcta ccaaatacta aactgatgtg ggcatccttg
      841 tattcaatat ttgtatatat tttacgttac ggtattgtct catggactcc taagttttta
      901 gcgacgagtg tgcaagatgg tggaaaaggt attactgcaa cagctggtat gggtggtttt
      961 tcactttttg aaataggtgg tattattggg atgctaacag caggatatct atctgccaaa
     1021 gtgtttaaaa actctaaacc tttaaccaat gtagccttct tagttgtagc aatcttatta
     1081 ctagctgcct attggtttat tccagcagga cctcaatata tggcattaga cttcattatt
     1141 ttactaggtt tgggggcatc aatctacggt cctgttatga tggttggcct gtatgctatg
     1201 gagttagttc caaaagcagc agctggagca gcttcaggat tgactggcac atttagttat
     1261 gtcggaggag caacgatcgc tactctagct attggtatta ttattgacca ctttggttgg
     1321 ggagtagcat ttataatatt tggtatttct ggatttgcgg ctattgtctg taccttgctt
     1381 tcaagagata agagtctaga gtattggtga tattatttgg taaaagtatc atttaatatt
     1441 tagtgagata agttatgaaa aaaggcctct tgaaagagac cttttttgtt ttagataatc
     1501 tatttgttga agtaattagt gatatcgatt gtttttaata gatagtttct acctagttaa
     1561 aataaaacta ccccattaat aatgattcaa ttttaatgtc atattcaggg tagtgtatgc
     1621 ttaaatgttc gataatcaca tgataaattt gtgtgatttt tgctcttttt tctgggaagt
     1681 catgaccaat atagtggaag gaacattcta tcgttaaaaa gagctcatgg aataaggtat
     1741 tgacttcttc taaaagttct aaaagttttt tatcttcagt aataaacgca ggtgcagtat
     1801 tgatttcatc agcccaagca tctacaggtc taagtggata agtaccgtat tctagacata
     1861 attcataagt cataaaagtc ctcctagtct tttactattt taacatatgt gtgttcttaa
     1921 gaagaataat ttgtctttaa agcaaagaat atgtcctaag cgtatttttt gctatgttgt
     1981 aaggagtcgt tatataatga agtatactaa cttattttgc agatgaggag aaaaaatgtc
     2041 agaaaaaatc agagttttac tctattataa atatgtttca attgaaaatg cggaagagta
     2101 cgctgcgaaa catctagaat tttgtaaatc tattgggctt aaaggtcgta ttttgattgc
     2161 ggatgagggg attaatggaa ctgtttctgg agattatgaa acaactcaga agtatatgga
     2221 ttgggtccac agcgatgagc gttttgctga tctttggttc aagattgacg aggaaaatca
     2281 acaagctttc cgtaaaatgt ttgttcgtta taagaaagag attgtgcacc ttgggttaga
     2341 agataataac tttgatagcg atattaatcc tttggaaaca acaggtgagt atttaaatcc
     2401 taaacaattt aaagaagcct tgcttgatga agatactgtt gttttagata cgcgtaatga
     2461 ttatgagtat gacttaggtc acttccgtgg agcaattcgt cctgatattc gcaatttccg
     2521 tgagttacca caatgggtac gtgacaacaa agataagttt atggaaaaac gtgttgttgt
     2581 gtactgtacc ggtggtgttc gttgtgagaa attctcgggt tggatggttc gagaaggttt
     2641 taaagatgtt ggacaattac acggtggtat tgcaacatat ggaaaagatc cggaagttca
     2701 aggtgaactt tgggatggtg cgatgtatgt ttttgatgat cgtatctcgg tgccaattaa
     2761 tcatgttaat ccaactgtta ttagtaaaga ttattttgat ggaacacctt gtgaacgtta
     2821 tgtcaattgt gcaaatccgt tttgtaacaa acaaattttt gcttcagaag aaaacgaagc
     2881 gaagtatgtt cgagggtgct cgcctgaatg tcgtgcacat gaacgtaatc gctatgtgca
     2941 agaaaatggt ttatcacgtc aagaatgggc agaacgttta gaagctattg gagaatcctt
     3001 acctcaagta gcaaatgttt aatattaaaa aatcgctagc aataaagcta gcgatttttt
     3061 agcaatcaca attgacatcg caacaagcta ggttatcaag gtacatttga ttttctttaa
     3121 agaattcaag ggtatcttcg tctaatataa tcccttctcg ttgtgcgata tgattaataa
     3181 cagggacaaa gcgctgtctg aatttataaa tatagcagaa aatagtatct ctttgtcgta
     3241 agcttggacc aattaagaaa cagttattaa cgatagtaga ttcgtcatct tcagtaacta
     3301 ttggcaactg atttttatcg tattcaaata aagcgattcc gtcaatgaga tgacaggtat
     3361 ttagaaaacc tgtggaaaga ataggtttat ggaaagactc cgcagtacta ccgtcatcaa
     3421 aagtaacttg atattgtttt ccaatttgtt taatctcaat ggctttcttg ccttcagaga
     3481 tactataata ttcttttttg tggtcttgta tatgttttaa acgttcttta gttagggggg
     3541 ataaagagat gcttggatcg ggattgctct ctttacgacc aaacgtatct gtatataatt
     3601 cgacttgatt tcccaaatat actagatgtg ttagtgcatc acaagcactt tcgtttccac
     3661 caatgatgat aaatggattg tcagatttga tatggaaatt atcaacttga ccatagtgca
     3721 ttcctagatc tgcaccttta atatcaatgg tatttggatt ttgaaattct cctgttgcca
     3781 taataagata atcagcagaa aaatctccct ttgaagtttt aataacaaaa aggctatctc
     3841 gtttatcaat agatagaaca gatgtttcgt tttggatagg gagattataa tgagcagcaa
     3901 ctaattgtaa gtagcgtgca tattccactc cagaaagatg ttctttttca aatgagaaag
     3961 caggagaagt atctggaata acagcgttta gatctggaaa accaaagcca ttggtagtga
     4021 aggaaggtgt gataaattgt gttgttctgg gccatcttaa aaaagattct ccaatgtgcc
     4081 ctttttcaat gataagaaaa tttgtaagtc ctaatcgttg catggcagaa ccaaaaccga
     4141 ttcctgcagc gcctgcaccg ataattatgg tttgataatg tttcatgtct tctccttgac
     4201 aaaaataaat gtgtaagata aaatacttaa ccagttaaca ataatacagt ttaacaaata
     4261 aaagagtgtt tgtaaagaaa ggactatttg tgtttttact attatgtatt ttatcgatgt
     4321 ggctcatgat ttttggggtt gcattctatt actttgggtc gctacatcaa tcattgactt
     4381 caaggattat ttatcagttt gtacttactg ttttgctaac gacaacagct tggttcatgg
     4441 gtgcctattt tttagcattt gaaggtcatt ttaaaactgt ctttcaattc caagaagcag
     4501 atggtaaaca aattgtaaat tgtttatttc aactttgttt cgccctttac gcagtagtaa
     4561 tgcttattgg gtcaattatt gatagagttc agacgaagcg acttttatta gctgttgttt
     4621 cttggctttt cttggtgtat acgcccttag cttatttaat ttggaattca gagggtgttt
     4681 ttgcaaagat gggagtactt gatttttcag gcggaatgat cgttcattta tctgcagggc
     4741 tttcttcgta tattttagct catgttattg gcaagagtga acatcagcac aataaggtta
     4801 aaaatgatag tctcttttta ggaatgatac ttattacatt tggttggttt ggatttaata
     4861 tgggtccagt tggtgagtgg aattcacaag ccattatgat acttttaaat actatatttg
     4921 ctattatagg tggtggtttg gcgtggacat tggctgctaa gtggaatggt gaggaagaga
     4981 aaacaggaag cttattgaat ggcattattg ttggtcttgt gacgagtaca gcaggagttg
     5041 ggtatctctt aacttggcaa ttattagctg taacattctt tgcatccctc ttcacctact
     5101 ttgtaacgga ttacgttgcg aaagcttttg ccattgatga tgtcgtttcc tcctttggaa
     5161 tgaatggtat tgggggattg ttgggaagtt taggagttgg tctctttaaa ttatcacata
     5221 tgccagttca actgttagca ttagctacca caattctttt gtcaattata atgacctata
     5281 tcatatctaa agcgattttt agaaaataat aaataaggtt gagatgtaca tctcaacctt
     5341 atttattatt ttagcaagga gagtgactaa atcgaattat tatcattttc ttactagaca
     5401 gcaactgtac aaggttctga aagttagttg attatt
//
LOCUS       DATRSD010000096         5326 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRSD010000096 DATRSD010000000
VERSION     DATRSD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.097"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            36..1193
                     /locus_tag="VBD85_001754"
     CDS             36..1193
                     /locus_tag="VBD85_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO7134293.1"
                     /translation="MTFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLATM
                     MPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLYV
                     EGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELQDKHEILQLNYDKAKYNL
                     LDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLSS
                     YLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKY
                     IKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIIFERANGLREL
                     FFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            1730..5182
                     /gene="scpA/B"
                     /locus_tag="VBD85_001755"
     CDS             1730..5182
                     /gene="scpA/B"
                     /locus_tag="VBD85_001755"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO7134294.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQT
                     VETPQPTAVSEEAPSSKETKTPQTPSDAGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKAKARYQSKEDLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAINLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETVTVKTADQQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALIE
                     RGDIDFKDKIAKAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDNP
                     QKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKTETKPEKDSSGQTPGKTPQKGQPS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRQK
                     ETKK"
BASE COUNT         1893 a          974 c         1036 g         1423 t
ORIGIN      
        1 agtctgaacg tacggttgtt ttctcttttg gagaaataac atttagtagg agtcgctgga
       61 caaatggctt tgaaactaga ataccagtag atgagtggtt aggtcttgaa aaatataaga
      121 gatattcaat agaattctta tatcatgttg caaaattggc tacaatgatg ccttatcgtc
      181 aagtttgcaa agtaatagat agcactttgc aaacaatcat aacaaaagac tgtgttttaa
      241 aagcagtaaa atttgtagaa aaattgttaa aagaaaaaga acgctatcgt ttttatttgg
      301 aagagccacc cgaacgtaaa aaagtgaaaa aactgtatgt tgagggtgat ggagtcatga
      361 ttaaaagcac agattctaga gaggaaagaa ggtatttaga tttaacacat tttgttattc
      421 atacaggctc aaaaaaagtt tctactaaaa gatatgaatt gcaggacaag cacgaaatat
      481 tacagcttaa ttatgataaa gctaaatata atcttttaga ttatatttat aataactatg
      541 aagtagatga cgatactatt ttaatcacta actctgatat gggtaaaggc tatactagta
      601 gagtttttaa ggaattagga aaagcactta aggtaaagaa acatgagcat ttttgggata
      661 tctatcatgt taaagaaaag ttaagttcat accttagaaa atatccaatt gaattaaccg
      721 attttgcttt agatgcggta aaaaaatata attctgataa gcttgaatta gtttttgata
      781 ctgttgaatc actgatttgt gatgaacttg aagatcaaga atttcagaag tttaagaaaa
      841 aagtattaaa taatttcaaa tatataaaac cagctcatct tagaaatctt tcaaatcgtg
      901 gtattggtat catggaatca caacacagaa agataacgta tagaatgaag cgacgtggca
      961 tgtattggtc aaagtgggga atctccacaa tggcaaatat gattatattt gaaagagcta
     1021 acggtttacg agaattattt ttcggttctt ggagaaaggt atacagtgag tataaagaag
     1081 gttcatttag tgcagggcga ctttttaaaa agacagatga attagataaa ttttctaagc
     1141 cccttctaaa aaatggcaga aaatggagta taacaggaat caaaacaaaa tagaaaaagt
     1201 ctaaaaatac ctttattttt agcgtttttt tgcatgtttt aacttgcatt aaatcttcta
     1261 aagtagtata attatattaa agaaaagcaa aaaatacttg ttttggtctt tttaagaata
     1321 attcaagttt tcttaaattt atgaggtatc gactaatctc aaaatttcag acacatacct
     1381 gagtgaacat caagagagaa ccgatcggtt ctctctttta tgatagaata atgagattaa
     1441 ggaggtcaca aaataagcaa ctcttaaaaa gctgaccttt actaataata atctttgttt
     1501 tataataaat atgttagtaa tataattaat aaatgagata catttaatca ttatgacaaa
     1561 agcaagaaaa atagctgtat catatgcaaa taacctcctg tttgctcttt aaaaaagacg
     1621 ttatccttat ttctccacgc acagatggat aactaggaga gaatcgtttg attctctctt
     1681 ttcttaatgg tcataaagac aaagtctctc atcatgaaag gacgacacat tgcgtaaaaa
     1741 acagaaacta ccatttgata aacttgccat tgcgcttatg tctacgagca tcttgctcaa
     1801 tgcacaatca gacatcaaag caaatactgt gacagaagac actcctgcta ccgaacaaac
     1861 cgtagaaact ccacaaccaa cagcggtttc tgaggaagca ccatcatcaa aggaaactaa
     1921 aaccccacaa actcctagtg atgcaggaga aacagtagca gatgacgcta atgatctagc
     1981 ccctcaagct cctgctaaaa ctgctgatac accagcaacc tcaaaagcga ctattaggga
     2041 tttgaacgac ccttctcagg tcaaaaccct gcaggaaaaa gcaggcaagg gagctgggac
     2101 tgttgttgca gtgattgatg ctggttttga taaaaatcat gaagcgtggc gcttaacaga
     2161 caaagccaaa gcacgttacc aatcaaaaga agatcttgaa aaagctaaaa aagagcacgg
     2221 tattacctat ggcgagtggg tcaatgataa ggttgcttac taccatgact atagtaaaga
     2281 tggtaaaacc gctgtcgatc aagagcacgg cacacacgtg tcaggaatct tgtcaggaaa
     2341 tgctccatct gaaacgaaag aaccttaccg cctagaaggt gcgatgcctg aggctcaatt
     2401 gcttttgatg cgtgtcgaaa ttgtaaatgg actagcagac tatgctcgta actacgctca
     2461 agctatcaga gatgctatca acttgggagc taaggtgatt aatatgagct ttggtaatgc
     2521 tgcactagct tacgccaacc ttccagacga aaccaaaaaa gcctttgact atgccaaatc
     2581 aaaaggtgtt agcattgtga cctcagctgg taatgatagt agctttgggg gcaagacccg
     2641 tctacctcta gcagatcatc ctgattatgg ggtggttggg acacctgcag cggcagactc
     2701 aacattgaca gttgcttctt acagcccaga taaacagctc actgaaactg ttacggtcaa
     2761 aacagccgat cagcaagata aagaaatgcc tgttctttca acaaaccgtt ttgagccaaa
     2821 caaggcttac gactatgctt atgctaatcg agggacgaaa gaggatgatt ttaaggatgt
     2881 caaaggtaag attgccctta ttgaacgtgg cgatattgat ttcaaagata agattgcaaa
     2941 agctaaaaaa gctggtgctg taggagtctt gatctatgac aatcaggaca agggcttccc
     3001 gattgaattg ccaaatgttg atcagatgcc tgcggccttt atcagtcgaa aagatggtct
     3061 cttattaaaa gacaatcccc aaaaaaccat caccttcaat gcgacaccta aggtattgcc
     3121 aacagcaagt ggcaccaaac taagccgctt ctcaagctgg ggtctgacag ctgacggtaa
     3181 tattaagcca gatattgcag cacccggcca agatattttg tcatcagtgg ctaacaacaa
     3241 gtatgccaaa ctttctggaa ctagtatgtc tgcgccatta gtagcgggta tcatgggact
     3301 gttgcaaaag cagtatgaga cacagtatcc tgatatgaca ccatcagagc gtcttgattt
     3361 agctaaaaaa gtattgatga gctcagcaac tgccttatat gatgaagatg aaaaagctta
     3421 tttttctcct cgccaacaag gagcaggagc agtcgatgct aaaaaagctt cagcagcaac
     3481 gatgtatgtg acagataagg ataatacctc aagcaaggtt cacctgaaca atgtttctga
     3541 taaatttgaa gtaacagtaa cagttcacaa caaatctgat aaacctcaag agttgtatta
     3601 ccaagcaact gttcaaacag ataaagtaga tggaaaacac tttgccttgg ctcctaaagc
     3661 attgtatgag acatcatggc aaaaaatcac aattccagcc aatagcagca aacaagtcac
     3721 cgttccaatc gatgctagtc gatttagcaa ggacttgctt gcccaaatga aaaatggcta
     3781 tttcttagaa ggttttgttc gtttcaaaca agatcctaaa aaagaagagc ttatgagcat
     3841 tccatatatt ggtttccgag gtgattttgg caatctgtca gccttagaaa aaccaatcta
     3901 tgatagcaaa gacggtagca gctactatca tgaagcaaat agtgatgcca aagaccaatt
     3961 agatggtgat ggattacagt tttacgctct gaaaaataac tttacagcac ttaccacaga
     4021 gtctaacccg tggacgatta ttaaagctgt caaagaaggg gttgaaaaca tagaggatat
     4081 cgaatcttca gagatcacag aaaccatttt tgcaggtact tttgcaaaac aagacgatga
     4141 tagccactac tatatccacc gtcacgctaa tggcaagcca tatgctgcga tctctccaaa
     4201 tggggacggt aacagagatt atgtccaatt ccaaggtact ttcttgcgta atgctaaaaa
     4261 ccttgtggct gaagtcttgg acaaagaagg aaatgttgtt tggacaagtg aggtaaccga
     4321 gcaagttgtt aaaaactaca acaatgactt ggcaagcaca cttggttcaa cccgttttga
     4381 aaaaacgcgt tgggacggta aagataaaga cggcaaagtt gttgctaacg gaacctacac
     4441 ctatcgtgtc cgctacactc cgattagctc aggtgcaaaa gaacaacaca ctgattttga
     4501 tgtgattgta gacaatacga cacctgaagt cgcaacatcg gcaacattct caacagaaga
     4561 tcgtcgtttg acacttgcat ctaaaccaaa aaccagccaa ccggtttacc gtgagcgtat
     4621 tgcttatact tacatggatg aggatctgcc aacaacagag tatatttctc caaatgaaga
     4681 tggtaccttt actcttcctg aagaggctga aacaatggaa ggcgctactg ttccattgaa
     4741 aatgtcagac tttacttatg ttgttgaaga tatggctggt aacatcactt atacaccagt
     4801 gactaagcta ttggagggcc actctaataa gccagaacaa gacggttcag atcaagcacc
     4861 agacaaaaaa ccagaagcta aaccagaaca agacggttca ggtcaaacac cagataaaaa
     4921 aacagaaact aaaccagaaa aagatagttc aggtcaaaca ccaggtaaaa ctcctcaaaa
     4981 aggtcaacct tctcgtactc tagagaaacg atcttctaag cgtgctttag ctacaaaagc
     5041 atcaacaaga gatcagttac caacgactaa tgacaaggat acaaatcgtt tacatctcct
     5101 taagttagtt atgaccactt tcttcttggg attagtagct catatattta aaacaaaacg
     5161 ccaaaaagaa actaaaaaat agtattactt aaactgataa gagcttattt aatagagaga
     5221 accaatttgg ttctctctat ttttttatat gtgctacctc atcaaaaaga agttaggaca
     5281 aatagctgtc aacaattgcc ttttgcttat cccttcagtt gatgtg
//
LOCUS       DATRSD010000097         5275 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRSD010000097 DATRSD010000000
VERSION     DATRSD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.098"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            52..612
                     /locus_tag="VBD85_001756"
     CDS             52..612
                     /locus_tag="VBD85_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7134295.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            613..2565
                     /gene="gyrB"
                     /locus_tag="VBD85_001757"
     CDS             613..2565
                     /gene="gyrB"
                     /locus_tag="VBD85_001757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO7134296.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            2659..4383
                     /gene="ezrA"
                     /locus_tag="VBD85_001758"
     CDS             2659..4383
                     /gene="ezrA"
                     /locus_tag="VBD85_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO7134297.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            4477..5118
                     /gene="serB"
                     /locus_tag="VBD85_001759"
     CDS             4477..5118
                     /gene="serB"
                     /locus_tag="VBD85_001759"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO7134298.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(5139..>5275)
                     /locus_tag="VBD85_001760"
     CDS             complement(5139..>5275)
                     /locus_tag="VBD85_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7134299.1"
                     /translation="AKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSLI
                     "
BASE COUNT         1801 a          781 c         1097 g         1596 t
ORIGIN      
        1 aagtaaggtg gtagctataa aaaatcgtaa taatatgata taataaaggg tatgaattat
       61 catgattata tttgggattt gggtggtaca ctacttgata attacgaaag ttccacacgt
      121 gcttttgtag agactttgaa agaatttgga tatcaagcag accacgatag tgtgtatcaa
      181 aaattaaaag agtcaacagc agatgcaatt gcatatttta tccctgaaga agctgatttt
      241 ttaaaagagt ataaggctaa tgaagctaag gttttggaaa caccaattct attccaaggg
      301 gcaaaagaac ttttagcgaa aattcaaaga caaggtagtc gtaatttttt ggtgtctcat
      361 cgtgataatc aagttatagt tattctggaa aaaacagaaa ttattgacta ttttacagaa
      421 gtagtgactg ctgataacgg cttttcaagg aaacctagtc ccgagagtat gctttatttg
      481 aaagaaaaat accaaattga taattgttta gtaataggcg atcgtgatat agataagcaa
      541 gcaggagaat cggctggttt tgatacctta ttagtcgatg ggtcaaaatc attgatggag
      601 ataatagagt aaatgacaga agaaacaaag aatatggaac aacgtgctca agaatatgac
      661 gcaagtcaga ttcaagtatt agaaggttta gaagctgttc gtatgcgtcc aggcatgtat
      721 atcggttcga cttcgaaaga agggcttcat caccttgtat gggaaattgt tgataactca
      781 attgatgaag ccttagctgg atttgcagga catattaaag tttacattga accagataac
      841 tctattacag ttgttgatga tggtcgcggt attccggtag atatccaaga aaaaactgga
      901 agaccagctg tagagacagt ttttacagtt ctccatgccg gtggtaaatt tggtggtggc
      961 ggatacaagg tttctggtgg cttacatggt gtagggtcat ctgttgttaa tgcattgtca
     1021 acacaattag atgtcaaggt ttataaaaat ggcaaggttc attatcaaga ataccaacgc
     1081 ggtgttgttg ttaatgatct tgaaattatc ggtgatactg acttatcagg aacaacagtt
     1141 cactttacgc ctgacccaga aattttcact gaaaccactg tatttgattt tgataaatta
     1201 gccaaacgta ttcaggaatt agctttttta aatcgtggac taagaatctc catttcagac
     1261 aagcgagaag gtcaagaagt tgaaaaagaa tatcactatg aaggtggtat aggttcatac
     1321 gttgagttta tcaatgagaa caaagaagtg atttttgaaa atcctattta tactgacggt
     1381 gaattagatg gtatttcagt tgaagtagca atgcagtata ctactggata tcaagagact
     1441 gttatgagtt ttgcaaataa catccacact catgaaggag gaacgcacga acaaggattt
     1501 cgtactgcct tgacacgtgt gattaatgat tacgctaaaa agaataagat tctcaaagaa
     1561 aatgaagata atttaactgg agaagatgtt cgtgaaggtt taacagcagt tatatctgtt
     1621 aaacatccaa atccacaatt tgaaggacaa actaaaacaa aacttggaaa ttctgaagtt
     1681 gttaaaatta caaatcgtct ctttagcgaa gctttcaatc gattcctatt agaaaatcca
     1741 caagtagcta agaagattgt tgaaaaagga attttggcat ctaaagcccg tattgcagca
     1801 aaacgggcgc gtgaagtaac acgtaaaaag tcaggcttag agatttcaaa cttacctggg
     1861 aaactggctg attgctcatc taataatgct gagatgaatg aactttttat agtggaggga
     1921 gattctgcgg gagggtctgc aaaatcagga cgtaatcgtg agtttcaagc aattttaccg
     1981 attaggggta agattctaaa cgttgaaaaa gctacaatgg ataaaatttt agcaaacgaa
     2041 gaaatccgaa gtctcttcac agctatggga actggatttg gtgcagattt tgatgtatca
     2101 aaagtcagat atcaaaaatt agtcattatg actgatgccg atgttgatgg agctcacatc
     2161 agaactttac ttttaactct gatttatcgt tttatgcgac ctgttttgga agcaggatat
     2221 gtttatatcg ctcaaccacc aatctatggt gtcaaagtag gaagtgagat taaagcttat
     2281 attcagccag gggtcaatca agaagaggaa ttacgtcaag ctctagatac ctatagttca
     2341 ggacgttcaa aacctactgt acaacgttac aaaggtctag gtgagatgga tgaccatcaa
     2401 ctttgggaaa caacaatgga tcctgaaaac cgcttgatgg cacgtgtctc agttgatgat
     2461 gcagcagaag cagataagat tttcgatatg ctaatgggag atcgagtaga accacgacgt
     2521 gaattcattg aagctaatgc tgtttatagt aacttagata tctagtccaa agatagcttt
     2581 gaaaaacagt tcaagtatgc tataatctta acgttatagt agatttttag aaaattagac
     2641 cattataagg agagttgaat gtcgagcgga atcatattgc tgcttgtggc tatagtcctg
     2701 cttgtgatta ttgcctacgt agtcggcgtc gtaataagaa aacgtaatga tacattaatt
     2761 gcaaacttag aaactagaaa acaagagtta gttgaccttc ctgtgcaaga ggagattgag
     2821 caagtcaaat tgcttcattt gattggccaa agtcaatcaa cttttagaga atggaaccaa
     2881 aaatggactg atttatcaac taattcattt aaagatattg attttcattt agttgaagct
     2941 gaaaatctta atgattcatt taattttgtg cgtgctaagc acgagattga taatgttgat
     3001 agtcagctaa ctattataga agaagatatt gtttcgattc gtgaagcttt agaagtttta
     3061 aaagagcaag aagagaaaaa tagtgcccgt gtaacacatg ctttggacct atacgaaaca
     3121 cttcaaaaat caatttctga aaaagaagat aattacggga caaccatgcc agaaattgaa
     3181 aagcagttga aaaatatcga ggctgaattt tcacattttg tgaccttaaa ctcaactggt
     3241 gacccgattg aagcttctga agttttgaat aaagcagagg agcatacgat tgctttaggg
     3301 caaattacag aacaaattcc agctattgtt gctaaattag aagacgattt tccagatcag
     3361 ttagatgatt tggagacagg atatcgtcgt cttttagagg aaaactacca tttccctgaa
     3421 aaggatattg aacaacgttt tcaagaagtt cgtgaggcta ttcgttcgaa ttcagatggt
     3481 ttagttagtt tggatctaga tcgtgctcgc gatgaaaatg aacatattca agaaaaaatt
     3541 gataaacttt atgatatttt tgaacgtgag attgctgctt ataaagtagc tcataaggat
     3601 agtaaaatta ttcctcaatt ccttgctcat gcaaaatcta acaatgaaca actggggcat
     3661 gaaatcaaac gattatctgc taaatatatc ttaaatgaaa atgaaagctt aagtttacgt
     3721 tccttcacaa atgatttgga agaaattgaa acaaaggttt taccttctgt agaaaatttt
     3781 ggtcaagaag cttcacctta tactcatcta caaattttat ttgagcgtac gttgaaaaca
     3841 ttgactactg ttgaagaaaa tcaaatggaa gtttttgaag ccgttaaaac tattgaaagc
     3901 gttgagacac gagcacgcca aaatatggat aagtatgtta acaagctcca tatgattaaa
     3961 cgtttcatgg aaaaacgcaa tcttccagga atcccgcaag acttcttaag cactttcttt
     4021 acaacaagtt ctcaaattga ggcacttatt aatgaactca gtcgcggtcg tattgatatt
     4081 gaggctgttt cacgtttaaa tgatgtgact acaaatgcta tcgctaattt agagcaagct
     4141 acatatctag ttgttcaaga tgcgactctc acagagcagt tactacagta ttcaaaccgt
     4201 tatcgtagtt ttgaacaaaa tgtgcaaaag agctttgaac aagctttgta cttatttgaa
     4261 gttgaacata actataaagc atctttcgat gaaatttcat acgcacttga gactgtggaa
     4321 ccaggtgtaa ctgaccgttt tgtgacgtct tatgaaaaga cacaagaacg tattcgtttc
     4381 taaaaaagaa gagttagtac tcttcttttt ttaattgcta tctatgaaag gtgctaattt
     4441 taaggtacaa tagtaaaaaa gttttggagg ttttaaatga ttaaaggttt gttagtcatg
     4501 gacgttgatt caaccttaat aatggaagaa gctattgatt tattagctat tgaagcaggg
     4561 gtggggaaac aggttgctgc actgactgac gctgcaatgc gtggcgagtt agattttgaa
     4621 gaagctttga agaaaagagt agcgctactt aaaggtttgc ctgttactat tttgacagat
     4681 atattatcct caatacattt tactccaggg gcttatgaat tgattaaaga gtgtcacaag
     4741 cgtcagatga aagttggtct tgtatcagga ggttttcacg agacgattga tatcttagct
     4801 aagcaattac aagttgatta tgtcaaagcg aatcgtcttg gtgtcaaagg tggttttctc
     4861 actggcgaag ttgagggaga gattgtcact aaagaagtga aaaaaataaa gttaaaagag
     4921 tgggcttctg aaaatcactt agacctttca caaaccatcg caatgggaga tggtgccaat
     4981 gatttaccaa tgatcaaatc cgctggggta ggaattgctt tttgtgcaaa accaattgtc
     5041 agagaagaag ctgcgtatca gattaatgaa aaaaatttgc ttaaggtatt tgatattatt
     5101 gatgcaaatg aaaactaggc ctttattaag cctagttttt agattaagga ttttaagagt
     5161 tttatttgag agggtattaa tttgttttta gataaaaata aatcaatata ttctggtttt
     5221 aaccagatat agtcaacggt ctctccttct tgcaatgtaa ttatatcttt tgcac
//
LOCUS       DATRSD010000098         5231 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRSD010000098 DATRSD010000000
VERSION     DATRSD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.099"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..1895)
                     /locus_tag="VBD85_001761"
     CDS             complement(<1..1895)
                     /locus_tag="VBD85_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134300.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAIS"
     gene            complement(1897..2649)
                     /locus_tag="VBD85_001762"
     CDS             complement(1897..2649)
                     /locus_tag="VBD85_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134301.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHCLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(2675..3638)
                     /locus_tag="VBD85_001763"
                     /pseudo
     CDS             complement(2675..3638)
                     /locus_tag="VBD85_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            3804..4292
                     /locus_tag="VBD85_001764"
     CDS             3804..4292
                     /locus_tag="VBD85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO7134302.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(4324..5172)
                     /locus_tag="VBD85_001765"
     CDS             complement(4324..5172)
                     /locus_tag="VBD85_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7134303.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDALATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQD
                     LDQPVW"
BASE COUNT         1719 a          919 c          798 g         1795 t
ORIGIN      
        1 atagaaatcg caagtactgt caaaccactt actacatata cgattgttga gttaagcacc
       61 ccaaatacca aaagcatctg ttttagcatt ggaatagcaa ctccaaagtg aataactgct
      121 accactaaag gttggaagaa gaaaatcata atttgagaat taatagtccg tttaactaat
      181 tttttgctca tacctacttc ttgtaagata cgataagatc tcttatcttc atgaccttct
      241 gagtattgct tatagtaaac aattagagct atacctagca aaaagcttat acctaacaga
      301 aatccagtaa ataataaccc accaaacatt tgataacctt cttttagaaa actctgttta
      361 gtgctaatat ttccaacata cttaccatct acctctatta tgtcgttttt agaaattgaa
      421 tttatctctt gactattcaa atccaaatat gccttaaagg tttttggaaa agtattgatg
      481 ttcttagtat aaggtaaata tgcttttcta atattgtcaa tttgtttgtt gtttgcaaat
      541 ataatcaaac ccgggttgta agtatttgta gtctctggga cataagcctc ttttaaattt
      601 tttacaacgt caaatttatt tcccaataga tttatttttt tcaaacggct atcacctttt
      661 tgaacaaagt aagccacttg gttatctttt aattttggta attggtgacc taaacgacgg
      721 aaatcattct gagtaattag gtacataaaa cccgtttttg tgatatcaac atgtttgacg
      781 tttttagatg tgatattaag ttcacttgat tgactaactg gcatcgaaat cattgtctga
      841 ttataagtga tagcttcctt agatgactta cctagtttct tcaaaatctt ttcttcaaat
      901 atatttttcg cgtcaccttt tgaattatct attgataaac ttactgattt tggaaatagt
      961 ccggtaacaa cattttgtgt atttgaataa agtgagactg ttgtagcaat agtaactaga
     1021 gccataacag ctaataaagt gatacttgct aaccctactg cattttgctt cattcgaaaa
     1081 atcatttgcg aagttgatac aaaatgctca gatttataat aataatgctt gttttgacgc
     1141 aaccttttta agtaccatgt cataaaacta atataaaaaa gataagtacc agcaataact
     1201 aaaagtactg caaagaagaa acgatagata acagctaatg caggtgcttt acctgaagta
     1261 agagccattg tataagcgat ggcgatagct actaacgcta aaattgcaag tataagatta
     1321 ccttttggtt ctttttctcc ctgttgtttc tttctaaaaa gacttaatgg ggatgataaa
     1381 tgaacatgtc gaataactgg aacttctaaa gtcagaaaaa tacctgtaaa tataacgata
     1441 caaataataa atggccataa acttaaacta agatttagtg catgatagtt aataatgttg
     1501 acaaaaatta aataaataaa tttagcaaaa aaagcactaa acagacttcc tatagaaata
     1561 agaaatatat aaataataaa cagctctaga ctagctacac gcgcaacttg acgtttattc
     1621 atccccaaaa tgttatacag tccaaattca ctactacgct gtttcatcaa gatattgtag
     1681 ctataatgtt ccatgactac cgcaaagatt gacaaaacaa tcactccaag aaaaagaacc
     1741 gttgccgctg tccccatatc tcttccaaca gcactcatta agattagtag cgtagaacaa
     1801 gtcaatgaat aaagtaataa actggctaag aggaatggta tgtactggtc tatagaatgt
     1861 tttaaattat gccaggctat tttgagataa aacatattat tcacccccta atatggcaga
     1921 cattgttaaa gaaatatctt tattgaattc gctattattc ttattaccac gataaagttg
     1981 gtgaaaaata cgtccatctt tgataaataa aacgcgtttg gcatgactag cagcatttgc
     2041 tgaatgggtt accatcaaaa tagtttgtcc atccaagtta atagtttcaa ataaattcag
     2101 caagtcttct gaattacggt aatctaacgc tgctgttggt tcatctgcta ataaaatttg
     2161 agggttggtt atcaaactac gagcaatcgc tacacgttgc ttttgcccac cagagagctc
     2221 gaaaggtctc ttatctaata agtcatcaat tctcagatga gaagataatt ctgacaaaca
     2281 atgatccatt tctttgtaac gtttacgatc aagaactaaa ggaagataga tattatctct
     2341 tacagagaga gtatccaaaa ggttgaagtc ctgaaagacg aaaccaagat ttttcaaacg
     2401 aaaactcgct aattttgcct ctttaatttt cgtaatatct tccccattta aaatcacttg
     2461 tccatttgtc ggtttttcta aagtagctaa aatatttagc agagttgttt ttcccgaacc
     2521 agactctccc ataatagcaa tgaattcccc ttgttcaact ttaaaatcga cgtcttgtag
     2581 agcccttgtc tcttcttttg aaaaacgtgt acgaaaaact ttttctaagt gattgatttc
     2641 taataacata taattctcct ttatttatta taccctaatt ttgattgatt ctactcttta
     2701 accaagactg tgactcttgt aaaggaaggc tggttacttg atctggtaag ataggggtgt
     2761 tctcgtattt atatccttgt cgatcactaa ctattcctgt agttaaagct aatctagcac
     2821 cgtcgtaaag catgaaagtt tcattaacag tcgtatatcc tgctgtagct tgaccaaagc
     2881 ttttaacatt aggtaatcct ttaaatgata aaaaagtcat ttctgccgaa ctagcagttt
     2941 tatgattagt tataatagcg attggaacat gcttagtatt gattgttttc ctagatattt
     3001 ttaatgcttc tagcggtata tttttcatcg tgattgtttt cttatttcca tacttatcag
     3061 tataatgaaa tagggtatca tttggtaaaa tagaggctac cccccccaat catagggatc
     3121 atattgccgc cagtattatt cgataagtct aaaatgagtc ccttataact tttttccatc
     3181 aatatatttt gtaatttacc actataatgg ctaatagatc gtttatctaa cttacttata
     3241 gaaggaatag taattaagag aatgtcttcg tttaatcttc gatattctgg atatcgacta
     3301 tctagggtat ctcttcgtac agtctctttg gataatattt gactatgttt accgcctcca
     3361 taccgtactg cttcctgtac aatgttgcga ctctcttggt aagattttga cgttttcaac
     3421 tttttttctg atcgttgtct tataatttgc cattgacgac cttgcgaata caaaccacgc
     3481 tgttcaacac gatctaaaat aacacgtcca taacgttcgc tactaggagg taatagataa
     3541 atatttaatg ctgaaccata ataatgaact accactccta aaattcctaa aactataagc
     3601 attggaacta caaaaagtaa aactattttt cgccgcatct cttgctcccc ctttctcttt
     3661 aagtctatta tatggcaaaa tggtttacta taaccttaca tcttaagacc tatttcttac
     3721 aaatttgaaa gttttaaaag ctttataaac tataattttg atataatatc ttacataaca
     3781 aatggaaagt acggtgaaaa catatggcca agacaagtaa aaaattgaca aaatctttag
     3841 ggcctattgg taaattaatt tcaataatcc ctgacacaac tgaacttatt ggaaaagcaa
     3901 ttgataattc tcgtccaatt attgaaaaag aacttgaccg tcgtcatgaa aaaaaacaga
     3961 cttatactca gcttgataat gtagtacacc ttgatatcaa tgatgcgcaa gtttacttag
     4021 agcaacgcca ttttaatgta tcaaggattg ttgctgaacc aaattcaaag tgggcaaccc
     4081 tacatcctaa tcaagttgtg aatatgtcgc ctaaagctgg tagggtcaga atcggttcat
     4141 tagtgaagtt gtactatatt acagaggata tccttgaaga aagtaagaca ctacttttat
     4201 ctaaatccga aaaaagtaaa gcacgacaac aacggttaac agaagtattg agtaccccta
     4261 gtcaattact caaaaaaaat cgaaataaat aagcatgatc gaattgacca tgcttattta
     4321 tttttaccaa acaggttgat ctaaatcttg agatgttttt tcgataactt ttttaaccgc
     4381 atctgtatgg taagctttca caacttcttt aatggcttta gcttttgatt tgtcttgcca
     4441 acctttttga gcaactaaaa ggttgtacca ttgttttgca tttttacttt ttggttcaat
     4501 aaaaatagct gatttaggat ttattcctgc ttctgtcaca aaatcattat taataactgc
     4561 tgcatctgtg gaatccaaag aacgtgcggt ctgtgctgca tctacttctt ttaaatctaa
     4621 agatttagga ttggaaacaa catctgacat tgttgctaat gcatcacctg atactttcaa
     4681 ttttagcaag cctgctgatt gtaacaagta gagagagcga ctttcgttaa ctgcatcgtt
     4741 tgggatagta atagttgctt tatttggaat ttcagaaact gtttggtatt tacctttacc
     4801 gttcttagta ccagagtata atctaaagga agtaaagtat gtctcagcaa cggaaacaag
     4861 attggtctta tttgctttat tccaattgtt taagtaatta taatgctgga aagcattaat
     4921 atcaatctca tcactttcta aagctttatt aggttgtgta taatctgtga attccgtaaa
     4981 ttttagctta acgccttttt tcttaactaa ttcttcgatt ttatcccaac gagctttttc
     5041 agttttactc tttaccattg ttccaactct aacgactttg gcgtcttcat ttttagactg
     5101 accacaggct cctaaaataa gtgctgaaat tactactgtt gtcgttaacc ctaatagctt
     5161 tttgattttc ataatttatt tctcctttga tatggttgta tgattaatca actgatacta
     5221 gtgtaacaaa a
//
LOCUS       DATRSD010000099         5009 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRSD010000099 DATRSD010000000
VERSION     DATRSD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.100"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(60..133)
                     /locus_tag="VBD85_001766"
     tRNA            complement(60..133)
                     /locus_tag="VBD85_001766"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Asn,seq:gtt)
     gene            complement(163..234)
                     /locus_tag="VBD85_001767"
     tRNA            complement(163..234)
                     /locus_tag="VBD85_001767"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Glu,seq:ttc)
     gene            427..500
                     /locus_tag="VBD85_001768"
     tRNA            427..500
                     /locus_tag="VBD85_001768"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:461..463,aa:Arg,seq:tct)
     gene            complement(573..1052)
                     /gene="rlmH"
                     /locus_tag="VBD85_001769"
     CDS             complement(573..1052)
                     /gene="rlmH"
                     /locus_tag="VBD85_001769"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO7134304.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1253..2482
                     /locus_tag="VBD85_001770"
     CDS             1253..2482
                     /locus_tag="VBD85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO7134305.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTIT
                     IKLTKTSKDLAKQRANN"
     gene            2580..3353
                     /locus_tag="VBD85_001771"
     CDS             2580..3353
                     /locus_tag="VBD85_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO7134306.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELANSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFANQKELNRIINTLK"
     gene            3562..4923
                     /gene="dnaA"
                     /locus_tag="VBD85_001772"
     CDS             3562..4923
                     /gene="dnaA"
                     /locus_tag="VBD85_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO7134307.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1762 a          869 c          848 g         1530 t
ORIGIN      
        1 tcatcctatt ttattaacaa aaaaacgccg acaaatcaac gtttagatgt gtcttatcac
       61 tccggcagca ggactcgaac ctgcgacatc atgattaaca gtcatgcgct actaccaact
      121 gagctatgcc ggataaataa aaaactgcaa agcagttatc aatagtccgt acgggattcg
      181 aacccgtgtt accgccgtga aaaggcggtg tcttaacccc ttgaccaacg gaccataggt
      241 taggtgtttc cttagtactc ttaatattat atcagaaaaa aaaggtttgt ctatactttt
      301 gttcaataat ttttacaaaa aaattgaacc tacttatgag caaaaaaaat acatttccaa
      361 ctcataaata gtacatatga aaacttgaca gcctgcctaa tattttggta gaataacaga
      421 gttgatggtt ccatagctca gctggataga gcattcgcct tctaagcgaa cggtcgcagg
      481 ttcgaatcct gctggaatca gataatgaaa gcctagatag attatctagg cttttttatt
      541 tgtttttagc ggttgtttca taaatggaac gattatttgt ggtaaggagt gccttgacga
      601 atcataaaag cacgataaat ttgttccatc aagactaatt tcataagttg atgaggtaag
      661 gttaacagtc caaaactcat taaataatca gcacgttttt tcactttttg agataaaccc
      721 aaactaccac caataacaaa agtaatagta gagtagccgg atatcatttt ttgatcgatt
      781 agatggctaa atgattcaga tggcaattgt ttcccttcta tagccaaacc aatgacataa
      841 tccctctcac ctattttact caaaatacgt tccccttcta tatctaaaat tctttgattt
      901 tcagatatac tagctttatc aggtgtcttt tcatcagcta gctcaattgt ctcaatttta
      961 gaaaagcgat tcagacgttt ctgatactct gcaacacctt ctttaaggta tttctctttc
     1021 aattttccaa ctgtaattat ttttaatttc atagctacat tataacataa tccacaatat
     1081 tttcacaatt tatccacatg tttatcctta catatcccct tattttatag gcattctata
     1141 actataatca aggttatcca caagttgtgg atatttcctt tttttagctt ttaaggtata
     1201 attaagaagc ttgtcataag attagtataa aaatttacgg aggtagctat ttgtgaaaaa
     1261 aaaattagtc tcatcacttc taaagtgttc tctaatcatt attgttagct ttgctggtgg
     1321 agcatttgct agttttgtca tgaatcataa tgacaatatt ccaaatggtg gtgtcactaa
     1381 aactagtaaa gtaaattata ataacataac gcctacaaca aaagctgtta aaaaggtaca
     1441 aaatagtgtt gtttctgtta tcaattataa acaacaagag agtcgttctg acctatcaga
     1501 cttctatagt catttttttg gtaatcaggg gggcaacact gataagggct tacaagttta
     1561 cggtgaaggc tctggagtca tctataaaaa agatggtaaa aatgcctatg ttgtcactaa
     1621 taaccacgtc attgatgggg ctaaacaaat tgaaattcaa ctagctgatg gctcaaaagc
     1681 agttgggaaa cttgttgggt cagataccta ctctgattta gccgtcgtca aaattccatc
     1741 agataaggtt tcaaatattg cagaatttgc tgattcatca aaactcaaca ttggtgaaac
     1801 tgctatagcg atcggaagcc ctcttggaac tgagtatgca aattctgtaa ctcaaggtat
     1861 tgtatctagt ttaaaaagaa ctgtaacaat gactaatgaa gaaggacaaa cagtttctac
     1921 aaatgctatc cagactgatg ctgctatcaa tcctggtaat tcaggtggag cacttatcaa
     1981 tattgaagga caggttattg gaattaattc tagtaaaatt tcttctacat caaatcaaac
     2041 ctcaggacaa tcgtcaggaa atagcgttga aggtatggga tttgccattc cttcaaatga
     2101 tgttgttaag attatcaatc aacttgagag taacggacaa gtagagagac ctgctctagg
     2161 tatttctatg gctggattaa gtaatttacc atccgatgtt attagtaaac tgaaaatccc
     2221 aagtaatgtt actaatggta ttgtagtagc atctatccaa tctggcatgc cagctcaagg
     2281 caaactaaag aaatacgatg tcattactaa agttgacgat aaagaagtag catctccaag
     2341 tgatttacaa agtttactct atggccacca ggtaggagat tccataacag taacctttta
     2401 ccgtggtgaa aataaacaaa caatcactat aaaacttact aaaactagta aagatttagc
     2461 taaacaacga gcaaataact aaatcaattc tagttatttt aactaagata tagtaaaatt
     2521 aaaggggttg gtcagataga ccaatccctt tagtaagtct atagaatctg aggtctaaaa
     2581 tggaatattt agaaacgatc aacatcaatc atattgctcc aaatccgtat caacctcgcc
     2641 tagaatttaa tacaaaagaa ctagaagaat tagccaactc tattaaaatt aatgggctta
     2701 tccaacccat tattgtaaga ccgtcagcag tctttggtta cgaactggta gccggagaac
     2761 gacggctaag agccgcaaag ttagctaaat tggaaagtat acctgcaatt ataaaatcct
     2821 ataataatga tgatagtatg caattagcta ttgtcgaaaa tttacaacga tctaatttgt
     2881 cacctattga agaggctaaa gcctactctc aacttctaca aaagaaaagt atgacacatg
     2941 aagagttagc aaaatatatg ggaaaatctc gtccctatat atcaaatacg atacgactac
     3001 taaatttacc gccactaatt acttcagcta ttgaagaagg aaaactttcc tctggtcatg
     3061 cgcgtgcact actcagtcta ccagatgcta gtcaacagaa agattggtat cagagaatcc
     3121 tcactgaaga tattagtgta agacgactag agaagttatt aaaacaagaa aagaaaacaa
     3181 atcataagtc acttcaaaat aaagatgtct ttctaaaaca tcaagaaaat gagttagctc
     3241 aatttctagg tagcaaagtg aaacttacta ttaataaaga tggtgctggc aatataaaga
     3301 ttgctttcgc taaccaaaaa gaattaaaca gaattatcaa cactctaaaa taagggtgtt
     3361 gattattttt ttatttaatc aaacttatcc acaaggtatt ttgctatttt tctgttgatt
     3421 ctctaagctt ttcttttttc cacagtctgt ggaaaacttt aattaacatt gttgatttta
     3481 ttcttcaaca tctgtggaaa actttatttt tttatggtac aatataacaa taattatcca
     3541 caagacaata aggaagaagc tatgacggaa aacgaacaac tattttggaa tagagtacta
     3601 gagctatctc gttctcaaat agcaccagca gcttatgaat tttttgttct agaggctaga
     3661 ctcctcaaaa ttgaacatca aactgcagtt attactttag ataacattga aatgaaaaag
     3721 ctattctggg aacaaaattt ggggcctgtt atcctaacag ctggttttga aattttcaat
     3781 gctgaaatta cagctaacta tgtctcaaac gatttacatt tacaagaaac tagtttttct
     3841 aactaccagc aatctagcaa tgaagtaaat actttaccaa ttagaaaaat cgactctaac
     3901 cttaaagaga aatatacttt tgctaatttt gttcaaggag atgaaaatag atgggctgtt
     3961 tcagcatcaa ttgctgtagc tgatagtcct ggcacgactt ataatcctct atttatctgg
     4021 gggggacctg gtctaggaaa gacgcatcta ctaaatgcta ttggaaatca agttttaaga
     4081 gataatccaa acgcgagggt tttatacatc actgctgaga attttattaa tgaatttgtc
     4141 agtcatattc gtttagattc gatggaagaa ttaaaagaaa agtttcgcaa cttggactta
     4201 ctcctgattg atgatattca gtcgcttgct aagaaaacct taggggggac ccaagaggag
     4261 ttcttcaata ctttcaatgc tttacataca aacgataaac aaatcgtatt gaccagtgac
     4321 cgaaatccaa atcaattaaa tgatctagaa gaacgtctag tcacgcgctt tagttgggga
     4381 ctcccagtaa atatcacacc acctgatttt gaaacacgag ttgctatttt aaccaataaa
     4441 attcaagaat atccttatga ttttcctcaa gataccattg aatacttagc aggagaattt
     4501 gattccaacg tacgtgaatt agaaggagcc ttgaaaaata ttagtctagt tgctgacttt
     4561 aagcatgcta aaactattac agtagatata gctgcagaag ctatcagagc acgtaaaaat
     4621 gatggtccta ttgttactgt cattcctata gaagaaattc aaatacaagt tggtaaattc
     4681 tatggcgtaa ctgtaaaaga gataaaagca actaaaagaa cacaagatat tgtccttgca
     4741 agacaggtag ccatgtactt agctcgtgag atgacagata acagtctccc aaaaataggt
     4801 aaagaatttg ggggacgaga tcactcaact gttctccacg cttataataa aataaaaaat
     4861 atggttgctc aagatgacaa cttacgaatt gagatagaaa ctatcaaaaa taaaatcaga
     4921 taggctttgt ggataagtat cagaaaaata cgagactttt ccacaggttg tgaacaagtt
     4981 attttcttta tgaatactaa agttataat
//
LOCUS       DATRSD010000100         4947 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRSD010000100 DATRSD010000000
VERSION     DATRSD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.101"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            40..3742
                     /locus_tag="VBD85_001773"
                     /pseudo
     CDS             40..3742
                     /locus_tag="VBD85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            3952..4860
                     /locus_tag="VBD85_001774"
     CDS             3952..4860
                     /locus_tag="VBD85_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7134308.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
BASE COUNT         1770 a          747 c          957 g         1473 t
ORIGIN      
        1 atatagtatg catactgatt tttggaggaa ataggaaaag tggataaaca tcactcaaaa
       61 aaggctattt taaagttaac acttataaca actagtattt tattaatgca tagcaatcaa
      121 gtgaatgcag aggagcaaga attaaaaaac caagagcaat cacctgtaat tgctaatgtt
      181 gctcaacagc catcgccatc ggtaactact aatactgttg aaaaaacatc tgtaacatct
      241 gcttctgcta gtaatacagc gaaagaaatg ggtgatacat ctgtaaaaaa tgacaaaaca
      301 gaagatgaat tattagaaga gttatctaaa aaccttgata cgtctaattt gggggctgat
      361 cttgaagaag aatatccctc taaaccagag acaaccaaca ataaagaaag caatgtagta
      421 acaaatgctt caactgcaat agcacagaaa gttccctcag catatgaaga ggtgaagcca
      481 gaaagcaagt catcacttgc tgttcttgat acatctaaaa taacaaaatt gcaagccata
      541 acccaaagag gaaagggaaa tgtagtagct attattgata ctggctttga tattaaccat
      601 gatatttttc gtttagatag cccaaaagat gataagcaca gctttaaaac taaggcagaa
      661 tttgaggaat taaaagcaaa acataatatc acttatggga aatgggttaa cgataagatt
      721 gtttttgcac ataactacgc caacaataca gaaacggtgg ctgatattgc agcagctatg
      781 aaagatggtt atgggtcaga agcaaagaat atttcgcatg gtacacacgt tgctggtatt
      841 tttgtaggta atagtaaacg tccagcaatc aatggtcttc ttttagaagg tgcagcgcca
      901 aatgctcaag tcttattaat gcgtattcca gataaaattg attcggacaa atttggtgaa
      961 gcatatgcta aagcaatcac agacgctgtt aatctaggag caaaaacgat taatatgagc
     1021 cttggaaaaa cagcagattc tttaattgct ctcaatgata aagttaaatt agcacttaaa
     1081 ttagcttctg agaagggcgt tgcagttgtt gtggctgccg gaaatgaagg tgcatttggt
     1141 atggattata gcaaaccatt atcaactaat cctgactacg gtacggttaa tagtccagct
     1201 atttctgaag atactttgag tgttgctagc tatgaatcac ttaaaactat cagtgaggtc
     1261 gttgaaacaa ctattgaagg taagttagtt aagttgccga ttgtgacttc taaacctttt
     1321 gacaaaggta aggcctacga tgtggtttat gccaattatg gtgcaaaaaa aagactttga
     1381 aggtaaggac tttaaaggta agattgcatt aattgagcgt ggtggtggac ttgattttat
     1441 gactaaaatc actcatgcta caaatgcagg tgttgttggt atcgttattt ttaacgatca
     1501 agaaaaacgt ggaaattttc taattcctta ccgtgaatta cctgtggggg ttattagtaa
     1561 agtagatggc gagcgtataa aaaatacttc aagtcagtta acatttaacc agagttttga
     1621 agtagttgat agccaaggtg gcaatcgtat gctggaacaa tcaagttggg gcgtgacagc
     1681 tgaaggagca atcaagcctg atgtaacagc ttctggcttt gaaatttatt cttcaaccta
     1741 taataatcaa taccaaacaa tgtctggtac aagtatggct tcaccacatg ttgcaggatt
     1801 aatgacaatg cttcaaagtc atttggctga gaaatataaa gggatgaatt tagattctaa
     1861 aaaattgcta gaattgtcta aaaacatcct catgagctca gcaacagcat tatatagtga
     1921 agaggataag gcgttttatt caccacgtca gcaaggtgca ggtgtagttg atgctgaaaa
     1981 agctatccaa gctcaatatt atgttactgg aaacgatggc aaagctaaaa ttaatctcaa
     2041 acgagtggga gataaatttg atatcacagt tacaattcat aaacttgtag aaggtgtcaa
     2101 agaattgtat tatcaagcta atgtagcaac agaacaagta aataaaggta aatttgccct
     2161 taaaccacaa gccttgctag atactaattg gcagaaagta attcttcgtg ataaagaaac
     2221 acaagttcga tttactattg attctagtca atttagtcag aaattaaaag aacagatggc
     2281 aaatggttat ttcttagaag gttttgtacg ttttaaagaa gccaaggata gtaatcagga
     2341 gttaatgagt attccttttg taggatttaa tggtgatttt gcgaacttac aagcacttga
     2401 aacaccgatt tataagacgc tttctaaagg tagtttctac tataaaccaa atgatacaac
     2461 tcataaagac caattggagt acaatgaatc agctcctttt gaaagcaaca actatactgc
     2521 cttgttaaca caatcagcgt cttggggcta tgttgattat gtcaaaaatg gtggggagtt
     2581 agaattagca ccggagagtc caaaaagaat tattttagga acttttgaga ataaggttga
     2641 ggataaaaca attcatcttt tggaaagaga tgcagcgaat aatccatatt ttgccatttc
     2701 tccaaataaa gatggaaata gggatgaaat cactccccag gcaactttct taagaaatgt
     2761 taaggatatt tctgctcaag ttctagatca aaatggaaat gttatttggc aaagtaaggt
     2821 tttaccatct tatcgtaaaa atttccataa taatccaaag caaagtgatg gtcattatcg
     2881 tatggatgcc cttcagtgga gtggtttaga taaggatggc aaagttgtag cagatggttt
     2941 ttatacttat cgtttacgtt acacaccagt agcagaagga gcaaatagtc aggagtcaga
     3001 ctttaaagtt caagtaagta ctaagtcacc aaatcttcct tcacgagctc agtttgatga
     3061 aactaatcga acattaagct tagccatgcc taaggaaagt agttatgttc ctacatatcg
     3121 tctacaatta gttttatctc atgttgtaaa agatgaagaa tatggagatg agacttctta
     3181 ccattatttc catatagatc aagaaggtaa agtgacactt cctaaaacag ttaagatagg
     3241 agagagtgag gttgcagtag accctaagac cttgacactt gttgtggaag ataaagctgg
     3301 taatttcgca acggtaaaat tgtctgacct cttgaataag gcagtagtat cagagaaaga
     3361 aaacgctata gtaatttcta acaatttcaa atattttgat aacttgaaaa aagaacctat
     3421 gtttatttct aaagaaggaa aagtagtaaa caagaatcta gaagaaatag cattagttaa
     3481 gccgcaaact acagttacta ctcaatcatt gtctaaagaa ataactcaat caggaaatga
     3541 gaaagtcctc acttctacaa acaataatag tagcagagta gctaagatca tatcacctaa
     3601 acataacggg gattctgtta accatacctt acctagtaca tcagatagag caacgaatgg
     3661 tctatttgtt ggtactttgg cattgttatc tagtttactt ctttacttga aacccaaaaa
     3721 gactaaaaat aatagtaaat aaaactaagt aaactactct gcaatatgat gaaaagttca
     3781 gtttaacatt atgttaagct ggacttttag ggtattgact cttatttttg attaatttta
     3841 tattgattaa tgacatataa tattataata ttataattac gattagtatg tgataccgtt
     3901 cactttgtaa gctttcataa gatgaactag ccttaaggag ttttgatagc aatgaaaacg
     3961 ataaaagatt ggcaaataaa tatttgctta gcgacctata atggtcaaaa ataccttcgc
     4021 cagcaattag atagcataat acaacaaggt tatactgact ggatttgttt aataagggac
     4081 gatggatcta ctgatgatac agtagctatt attaaagaat atgttaatag agattcacgt
     4141 tttatattta ttaattctaa cgatgacaga aagttaggaa gtcaccgcag tttttacgag
     4201 ttggttaact ataaaaaagc agatttttat gttttctcag accaggatga tgtttggaaa
     4261 gaaaatcgtt tagaacgtta ccttgaagaa gcagagaaat ttaaccaaga attaccattg
     4321 cttgtttatt cgaactggac ttctgtagat gaaaaattaa cagtattgaa agaacataat
     4381 ccagcaacag ttattcaaga acaaattgcc tttaaccaaa taaatggtat ggttatcatg
     4441 atgaatcatg agttagcaaa gctttgggaa tatcgtcaaa ttggcgccca tgattcttat
     4501 gttggaactc tggcttacgc cgttggaaat gtggcttata tttctgacag tacagtcctt
     4561 tggcgtagac aagtaggggc agagtcgcta aacaattacg ggagacaata tggggtggct
     4621 acgttttggc agatgataaa tacttctttt gatcgtgctt cgcttatttt tgctcaagta
     4681 tccgataaaa tgtccctgga gcgtaaactc ttctttagtc gttttataga attaaaaaat
     4741 gccaatctaa taagaaggat ttatctttta tcaaaattaa aacttcgtcg gaaatcattg
     4801 aaagaaacag ttgctatgac tatcctactt ttaacaggtt acggtaaacc aaaagcataa
     4861 tttttttggt tgaaggaaat caagttgagt tgtagttaaa aatatagaaa atatttattt
     4921 ttcaaaaagt tgttgacact taacgaa
//
LOCUS       DATRSD010000101         4928 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRSD010000101 DATRSD010000000
VERSION     DATRSD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.102"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(29..238)
                     /locus_tag="VBD85_001775"
     CDS             complement(29..238)
                     /locus_tag="VBD85_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134309.1"
                     /translation="MIESFGRSILESSDEDMKSNPQNPVVRLYDDNELIGKFSLATLE
                     VVEDIDLADYNIKFAEFEMKNIQKE"
     gene            complement(327..467)
                     /locus_tag="VBD85_001776"
     CDS             complement(327..467)
                     /locus_tag="VBD85_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEO7134310.1"
                     /translation="MTDEEFADLVEQKSIGKKLNIHNKEVIEEFFGDEDFSEYEDVFN
                     TQ"
     gene            complement(490..1620)
                     /locus_tag="VBD85_001777"
     CDS             complement(490..1620)
                     /locus_tag="VBD85_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7134311.1"
                     /translation="MANARYRRRGNQNLWAYEIREEGKTVAYNSGFKTKKLAEAEAEP
                     ILQKLRTGSIITKNISLPELYQEWLDLKIMPSNRSDVTKKKYLSRKVTLEKLFGDKPI
                     SQIRPSEYQRIMNNYGQRVSRNFLGRLNTGVKQSLQMAIADKVMIEDFTQNVELFSTV
                     KSQDADSKYLHSEKAYLDLINAVKDKFNYKKSVVPYIIYFLLKTGMRYGELIALTWED
                     IDFDKGIFKTYRRFNSETSQFVPPKNKTSIRIVPVDNECLEILKNLKIEQNQSNKELG
                     LQNTNNMVFQHFGYPNSVPSTNGTNKVLRGIVQELNIEPIITTKGARHTYGSFLWHRE
                     YDLGIIAKILGHKDISMLIEVYGHTLEEKIQEEYNDIKQLWQ"
     gene            complement(1624..1809)
                     /locus_tag="VBD85_001778"
     CDS             complement(1624..1809)
                     /locus_tag="VBD85_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEO7134312.1"
                     /translation="MTNNVMTNKDLIEMGYRPSTANHIIHQARNLMVERGYTFYDRKR
                     LMVVPRSAVSEVIGLEV"
     gene            complement(1870..2898)
                     /locus_tag="VBD85_001779"
     CDS             complement(1870..2898)
                     /locus_tag="VBD85_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7134313.1"
                     /translation="MFNSVKIDYFAVTVKDVTPEYVIEKLLLIPLTNFTLNEWGVNKY
                     QRHYACSEIKVYFNLDPNSSMGVHVELKGQGCRQYEEFIEGNDNNWTNLVKRLIDNNS
                     NFTRLDIANDIFDESLNVQRLYEYSKKGLCITTARHAEYHEKFVIDSGELVGETVVFG
                     ARGNQQWCVYNKLMEQNGKLQTDIDINSWVRAELRCWQEKANLIAYQLNDMRPLASIY
                     FEAINGHYRFVSPKARDKNKRRRESVRWWQNYINTEEKTRLSIVREKPTLRQSEAWTD
                     KQVSKTIAKVYMAKYEAYGIDQAEVFLQDLLRRGVEKFTDNDEKEIEQYVREQQSSEY
                     WGIKKADL"
     gene            complement(2901..3320)
                     /locus_tag="VBD85_001780"
     CDS             complement(2901..3320)
                     /locus_tag="VBD85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134314.1"
                     /translation="MVTIKLTDTVSLGDLNLEKSQFEIPKKVIILSARINNKYNNING
                     ESVQTDEISKITCQVQDAEKVKVLKEMGMSADDLKTIPLEILDNLDKISKIASNDGLI
                     EKTIELINPQVRLAWNINRKGWGGIKLTTSDIKIIGE"
     gene            complement(3646..3819)
                     /locus_tag="VBD85_001781"
     CDS             complement(3646..3819)
                     /locus_tag="VBD85_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134315.1"
                     /translation="MLSDDFYKLLFEAEDSIFQLKKSNQSLQKQVKELTDRLDILENE
                     KGSGLFNKIKRSL"
     gene            4276..4770
                     /locus_tag="VBD85_001782"
     CDS             4276..4770
                     /locus_tag="VBD85_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134316.1"
                     /translation="MTFAKRLRELRQLNNITQIEMANKLGLNRVTYTNYEREKSEPSI
                     STLKEIATIFNVSIDYLIDFEDDTKGKNNRKKLLLSLEAELKKINRSFELEIKSLLID
                     LLNLIKTNQLSSCDVTKWLIDINEKDLECSSEILRFISEMPKEFEPIIDIIEIYQALN
                     KNDE"
BASE COUNT         1526 a          907 c          626 g         1869 t
ORIGIN      
        1 ctatttgtat ggtttcccca aaatccaact attctttctg aatatttttc atttcaaatt
       61 cagcaaactt aatgttgtaa tctgccaaat caatatcctc aactacctct aaagttgcta
      121 atgaaaattt tcctattaat tcattatcat catacaatct aacaactgga ttttgaggat
      181 tggatttcat atcttcatca gatgattcta atatgcttct accaaatgat tctatcataa
      241 aatgtctctg cttttcattt catttaatag tcaaaacatc tcttatcctc aatttattaa
      301 agcgaaaact ctaacataac atgaaattat tgagtattaa aaacatcctc atactcagaa
      361 aaatcttcat caccaaaaaa ttcttcaata acttctttat tatggatatt taattttttg
      421 ccaatacttt tttgttctac caagtctgca aattcttcat ctgtcatcta ttaatccctt
      481 attattaaat tattgccata gctgttttat atcattgtac tcttcttgaa ttttttcttc
      541 aagtgtatgc ccatagactt caattagcat agatatatct ttatgaccaa gaattttcgc
      601 aataattcct agatcatatt ctctatgcca taggaagctt ccataggtgt gtctagcacc
      661 ttttgttgta atgatgggtt ctatattcaa ctcttgaacg atgcctctta aaaccttatt
      721 tgtaccgttt gtactcggta cactattagg ataaccaaaa tgttgaaata ccatattgtt
      781 tgtattttgt aatccaagct ctttatttga ttgattttgt tcaatcttaa gatttttcaa
      841 gatttctaga cactcattat cgactggaac aattctgatt gaggttttat ttttaggtgg
      901 aacaaactga cttgtttcag agttaaatct tctatatgtt ttaaagattc ccttatcaaa
      961 atcaatatct tcccaagtta aagcaattaa ttctccgtat ctcatacctg tttttaataa
     1021 aaaatagata atatatggta ctaccgattt cttataattg aatttatctt ttacagcatt
     1081 aatgagatcc aaataagctt tttcactatg aaggtattta ctatctgcat cctgactttt
     1141 tactgttgag aataattcta cattttgagt aaaatcttct atcataacct tatcagcaat
     1201 agccatttgc aaactctgtt tcacgccagt atttaaccgg cctaagaaat tgcgactaac
     1261 tcgttgtcca tagttattca taattcgttg atattcacta ggtctgattt gagaaatggg
     1321 tttatcacca aatagctttt caagcgttac cttccgacta agatatttct ttttagtcac
     1381 atcacttcta ttactaggca tgatttttaa atctagccat tcttgataaa gttctggtaa
     1441 tgaaatattt tttgtgatta tgctaccagt tctcaatttt tggagaatag gctctgcttc
     1501 tgcctctgct agtttctttg ttttaaagcc actattataa gcaactgttt taccttcctc
     1561 acggatttca taagcccata aattctggtt tcctctcctt ctatatcttg catttgccat
     1621 atgttatacc tcaagtccaa tcacttctga aactgcactt ctagggacaa ccataagacg
     1681 tttacgatca tagaaagtat aaccacgttc caccattaaa tttctagctt gatggataat
     1741 atggtttgct gttgaaggac gatagcccat ttcaattaag tctttattag tcattacatt
     1801 atttgtcatt gtattttttt cctttcgtgt tataatttat ctagaatatt ttttctatgt
     1861 cgacttcttt cacaagtcgg ctttttttat gccccaatat tcagagcttt gctgttcacg
     1921 tacatattgt tcaatctcct tttcgtcatt atcagtaaac ttttcaactc ccctgcgaag
     1981 taagtcttgt aaaaagacct ctgcctgatc gataccgtag gcttcgtatt ttgccatata
     2041 aacctttgca attgtctttg acacttgttt atcagtccac gcttcagact gccttaatgt
     2101 tggtttttct ctcacaatac tcagtcgtgt tttctcctct gtattgatat agttttgcca
     2161 ccatcttaca gattctcttc ttcgtttatt tttatctctt gcttttggag aaacaaaacg
     2221 gtaatgtcca ttgatagcct caaaatatat agaggcgaga gggcgcatat catttagctg
     2281 ataagcaatc agattagcct tctcttgcca acatcttagc tcagctcgaa cccatgaatt
     2341 aatgtcaata tccgtctgta attttccatt ttgctccatt aatttattat aaacacacca
     2401 ttgttggttt cctcttgcac caaagacaac tgtttcacca acgagttcac ctgaatcaat
     2461 aacgaatttt tcatggtact ctgcatgtct cgcagttgtt atacataagc cttttttact
     2521 atactcataa agcctttgaa catttaacga ctcatcaaaa atatcattcg caatatctaa
     2581 tctggtaaag ttcgaattat tatcaattag tctctttact aaattagtcc aattattatc
     2641 attaccttct ataaattcct catactgccg acagccttgt ccttttagtt caacatgaac
     2701 tcccatgcta ctattaggat ctaagttaaa gtaaactttt atctctgaac aagcatagtg
     2761 acgttgatac ttattgactc cccattcgtt caaagtaaaa ttagttaaag gtattaataa
     2821 aagtttctct attacatact ctggagtgac atcttttact gtaacagcga agtaatctat
     2881 cttaacgctg ttaaacaatc ttattcacct ataattttga tatctgaggt agtcagtttt
     2941 attccacccc aaccttttcg atttatattc catgctagac gaacttgggg attaatcaat
     3001 tcaatcgttt tttcaataag cccatcattg cttgcaattt tactaatctt atcaagatta
     3061 tccaagattt ctaatgggat tgtttttaag tcgtcggcag acattcccat ttcttttaag
     3121 actttaactt tttccgcatc ttgtacttgg caagttattt ttgaaatttc atcagtttga
     3181 actgattcac cattgatatt attatattta ttgttaatac gtgcggataa gataattact
     3241 ttttttggaa tttcaaattg agatttttcc aagtttaagt cacctagact tactgtatct
     3301 gttaatttaa ttgttaccat ttttctacct cattttcata ataaattatt tgcatctttt
     3361 cttcctgtac acgaaagtta ctatcagccc tactctctca atggatttca gcatttaatg
     3421 gtgttgaacc accttcaccc cttatgtatc aagggtttga gcactatttc atgtacgtgt
     3481 gtacccccta ttagtagttg ggtaattatt ttcattgaaa tttcaaaaga atttttatag
     3541 attattaact tatcaaactt cggtttgtcc tcgagcactc tacatttatc tcccgagtcg
     3601 ctatggcttt cagcacctcg ctcctcgtta gataatcgat tgctctcata aacttcgttt
     3661 gattttgtta aataaacctg aacctttttc attttctaaa atatctaatc gatctgtcaa
     3721 ttccttaact tgtttctgta aagactgatt agattttttc agttgaaata tactatcttc
     3781 tgcttcaaat aataatttat aaaaatcatc tgatagcatt tctaattcac tttttatatc
     3841 tcgttcggac tgagcttttg agtcgcccca gacttcttta atagagtcat ctaatttctt
     3901 tgtcttatct actagcttag ataaggaaat gaatttagta acttctgctt ctgtaaaatc
     3961 aaagaaaact tgtgtgctac gctttgaaac tcttcttctg acttttttaa atctgtatcc
     4021 atataaagtt tcaatacgta atcgccattt cttgattgta ctaaccgact tgtttgtttt
     4081 taaagcaatt tgtttgtaat ctatagcatc aactccttaa ataaatatat tactttacaa
     4141 aaacgtgaat aaagtatata attcatttca taaactgaat aagaagaaat attttatata
     4201 cactaattgt atatagtatt ttgttctttg tcaaattaaa atgtacaaaa tactaacaaa
     4261 aaggagagaa ataaaatgac atttgcaaaa agactaagag aactccgcca acttaataat
     4321 attacccaaa tagagatggc taacaaactc ggactaaatc gagtaactta taccaattac
     4381 gaaagagaaa aaagtgaacc aagcatatct actttgaagg aaattgcaac aatttttaac
     4441 gtcagtattg actacctaat tgattttgaa gacgatacaa aaggaaaaaa taatagaaaa
     4501 aaactacttc ttagtttaga agcagaacta aaaaaaatta atagaagctt tgaactagaa
     4561 atcaaaagtt tactcataga tctactaaac ttaattaaaa ctaaccaact gagtagctgt
     4621 gatgttacaa aatggttaat agatattaat gaaaaagatc tagaatgctc aagtgaaata
     4681 cttcgtttta tttcagaaat gcccaaagaa tttgaaccta taattgatat cattgaaata
     4741 tatcaagctt taaataaaaa cgatgaataa taaaagtaga atgactttag ttggtcattc
     4801 tacttttatt attccttttc agcctcagat ttccaagttc tatttttgat tttagtatat
     4861 cacaatttag ttagtgttat tacaatttat gaaaaattca ataaaactta tcatgataaa
     4921 attccatc
//
LOCUS       DATRSD010000102         4848 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRSD010000102 DATRSD010000000
VERSION     DATRSD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.103"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            173..592
                     /locus_tag="VBD85_001783"
     CDS             173..592
                     /locus_tag="VBD85_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7134317.1"
                     /translation="MDNCIFCKIISGEIPSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            589..885
                     /locus_tag="VBD85_001784"
     CDS             589..885
                     /locus_tag="VBD85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134318.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(949..2256)
                     /locus_tag="VBD85_001785"
     CDS             complement(949..2256)
                     /locus_tag="VBD85_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7134319.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQNYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNHNGAATPNPNTGTQPVPGQTNP"
     gene            complement(2265..2825)
                     /locus_tag="VBD85_001786"
     CDS             complement(2265..2825)
                     /locus_tag="VBD85_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134320.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASAILEFLNTVTEET
                     DFILHTVSNQLSLSEMETFIENTLMTKNCICLIAKLKNKVIGLITIISQSDIEIEHVG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFGFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2779..3288)
                     /gene="tsaE"
                     /locus_tag="VBD85_001787"
     CDS             complement(2779..3288)
                     /gene="tsaE"
                     /locus_tag="VBD85_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO7134321.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3371..4792)
                     /locus_tag="VBD85_001788"
     CDS             complement(3371..4792)
                     /locus_tag="VBD85_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO7134322.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
BASE COUNT         1613 a          935 c          743 g         1557 t
ORIGIN      
        1 aattgcaata ccctttaggt catgctaaga taagtatgat ggtaaatata gatatcacaa
       61 tcaatacggg ctactttcat ttccctgtac gacttatgac ctatcaaacg ataaaaattt
      121 accattcctt aatagctacc caataaattt ttcaacatga aaggaattag ttatggataa
      181 ttgtattttt tgtaaaatca tcagtggaga aatcccttca tcaaaagttt atgaagatga
      241 tgaagtatta gcttttctcg acattacaca aactacgacc gggcacaccc ttcttattcc
      301 taaaaaacat gttcgaaatg tgttagagat ggatgaaaaa actgcacaga taacctttga
      361 acgcttgcct aaagtggcgc gtgctgtcca agctgctaca aaagccaaag gaatgaatat
      421 tattaacaac aatgaggaaa ttgctggaca aactgttttt catgcacacg tccaccttgt
      481 tccccgcttt gatgaatcag acggtatcaa aattcactac actactcatg aacccgactt
      541 tgaggcactt gcaaaattag ccaaagaaat tagaaaagag attcaattat gaaaataggt
      601 cgtttaattg cactaggttt agttagttta ggagcgcttg aactttacaa aaatagaaaa
      661 actatcaagg attcctatca aaatacaaaa aatgagacag attcagctaa attaaaatta
      721 gaacgtatca aaaatgatct tgctattatt agtcaggaaa aagaaaaaat cagattaatt
      781 tctcaagaac tcaatcataa attccaagtg tttaataaag atattcaacc tcgtttagaa
      841 gaaattaatc aacgcatggc taaatatcaa gagaaagacg aataagccga cggtataaaa
      901 atatctactc taaatataaa aagagcaaat aatgctcttt tttatatttt atggattagt
      961 ttgacctggt actggttgcg ttcctgtgtt tggattaggc gttgcagccc cgttatgatt
     1021 attgacactt ccacttgaat cagcctgacc tgtgttagtg ttactgctat agttactagc
     1081 aggagtgcta ctattatagt aattttgatg agttgtttga ttagtctcag aactataagt
     1141 agtattacca ctgtaacttg gtggtgcttc tgaataaggt gttgtattat aattattctc
     1201 ttgtgttgat gaataagtag aagaatcatt actagcagta gtaccatagt aatcttcata
     1261 tagaatcgcg cttgtcttca gagttttact acgcttttta tccagttctt tcttaattct
     1321 attttgaact gcaagtagat gtttcttagt taaaatttga taagagccac catctgataa
     1381 agtagcgtct tcacccttca actgataaga tttaatatgt tccaatgaat ctttataagc
     1441 taacaaatta ggaatcgttt ttgatgatat ctcaatatta gtttgcatgt tattacttac
     1501 tgcggaaaga atttttttgt atgaactaat actatttaac gccaatattt ttttaaggac
     1561 tttttgaatt acttcacgtt gtcttttttg acgcccataa tctccctctg gatcatcata
     1621 gcgcatacga gaataaacaa gtgcttgttc tccatttatt ttatgtgtcc ctggttcaac
     1681 aacagccttg tactctggtt cattggcagc aattgatatt ggaaagtcaa atttattagt
     1741 tactgttata ccaccaacag cattgactaa atcaactaat ccttgcatat taatttgcat
     1801 aaagtaatca acattaatat ctaataagtc ttgaacagtc atcaatgcca tttccgcacc
     1861 accagaagca taggctgcat ttagctttgc ttctactcca gtctgtccat tatttttggg
     1921 accactcaat ttaatcaata cgtcacgttc taagcttgtc atcgttgttt tattagtttt
     1981 aggatttata gtgactaaga tcatagaatc gctatttcct gaccacttag attttcgatg
     2041 ctctgaacct gtgtccaccc ccattaatag tattgaaaac ggctttgttt cttcaatagc
     2101 atgacttttt gatttagcta gcttaaaatc tttaaaagtc ttagacaatt cattagtcga
     2161 aaaattataa gctgaggcaa catagactcc caatgcaatt actgttgtta aaataattgc
     2221 agaaaacatt aaggttattt ttttccaaat tttcatgtat attcctaatc tatcaactta
     2281 ctcattctgt aaatgtctag aaattctcca ttttctcttt taatacctct tgtttgaagc
     2341 ccatctattt caaaaccgaa tttttggtag aggtgaatag cacgttcatt acgtccttgg
     2401 actgatagtt ctaagcggcg tgtaatatcg ttatctgacg cccattctat tgcttcttcc
     2461 atgagaatat gaccaatacc atatccccaa taatccttct gaacggcaat aaaaagatcc
     2521 ccaacgtgtt caatttcaat atcagactga ctaatgattg ttatcaagcc gatcacttta
     2581 ttttttaatt tagcaatcaa acaaatgcaa tttttcgtca ttaaggtatt ttcaataaac
     2641 gtttccattt ctgacaaaga tagttgattg ctcaccgtat gcaaaataaa atctgtttcc
     2701 tcagtaacag tatttaaaaa ttcaaggatc gcagatgctt cctctctcga agcttctata
     2761 aaatcaacag caaattgttt agacatcttg aatcgcctca ataatttctc ttgcgcgatg
     2821 cccataagct tctaactgaa cctgacgtcc ttgattgctc ctcgtaataa caatttctaa
     2881 gtagttattg ataaggttat cactaagcaa ctctccccat tcaataactg ttactccttg
     2941 accaaacaag aagtcatcta agtcaataga atcaggatca tctccaatac gataaacatc
     3001 caaatggtaa agtggtacac gcccttcata ttctctaaca atagtgtaag ttgggctctt
     3061 aatcatttgt tttatgtcta atccctttgc aatacctttt gtaagagttg ttttgcctgc
     3121 acctaaatta ccagtcaata atacgatatc tcctgacttt aaaacagtac ccagtttttg
     3181 ccctagggca ataagctctt cttcattttg agtataaaac atagagatat tataccagaa
     3241 atatctctgt cttccaacag tttgctgatt ctgacagccc catagcatat atttcttcac
     3301 ttttcttaag aaaaaaggaa accaggctac aaatctgatt tccttttatc ttatttatca
     3361 aactataata ttacaaaata gccaagctga taaagtttag aataaataat atatctaaaa
     3421 tccacagtac cacatgaata tcttttgctt gtcctttgat gactttagct agtgtatagg
     3481 tcaagaaacc agctgcaata ccataagtaa tgctataagt aaaccccata aaaagagaag
     3541 taaagaaagc tgggatagct tctgacatat catcccattt gatatctttt aaatttgaaa
     3601 gcatcataat accaacaata accaatattg gtgcagtagc ttgactcgga acgatagata
     3661 ctagtggact aaaaaaacta gatatagcaa acaaaccagc aactactaag gctgtcaatc
     3721 ctgtacgacc accagctccg attcctgctg ctgactcaac ataggttgta acgttagatg
     3781 ttccacaaat agcaccaaaa gttgtcccaa ttaagtctga atagagtgct ttgtctaaac
     3841 tcttagattc atgattatca ccagtcgttg ctaagatacc aactttttct ccggtaccaa
     3901 tgagcgttcc aatggtatca aaaatatcag tcagtgaaaa agcaagaatg gccattaata
     3961 cacttggtag acgtgaagga ttagaaaata aagaaattaa tcctttctct cctaatgcaa
     4021 ctccaaatac ttgcttcaaa tctctaaaag aggccgagag gttagttgct tcccaattaa
     4081 tagcatctaa cttaaccact cccattaaaa tacctaagag ggtcgttgtt aatatcgata
     4141 gaataatccc tccacggata ccctttacaa taaagaagat ggtaatgctt aaaccaatca
     4201 aagataataa aatggctgga ttatcaaacg acactagccc tggcgttgct gaagaatttg
     4261 ctgtaatagt agccaatcct ttcgcagcac cctttccaac aacatcatac gtcccaggat
     4321 caattgaaaa tttcaaaaaa ccagcatttt taatcccaac gtatgctaaa aaagttccga
     4381 ttcctgctgt aatggctgat tttaaagttg ttgggataga ctcaataatc atctttctaa
     4441 ctttcgttaa agtaataatc agagaaatca aaccacaaat aaataccata gctaaggctt
     4501 cttgccatgt atatcccaat gcaaagacaa ctgtatatgt aaagaaagca tttaatccca
     4561 tcccaggagc ttgtgcatag ggaaggttag catagaaagc cataacagat gtagctacaa
     4621 cggcaccaat aatcgtcgct aagaaaactc cttgtgctgg catcccagtc tgagataaaa
     4681 tagctggatt tacaaacaaa atgtacgaca ttgcaaagaa agtcgttaga ccagcagtga
     4741 tttcagtccg aattgtcgtc ccgtgttcct ttagcttaaa aaacttttcc attttaaaat
     4801 cggattctcc tttatttttt gctattttac acttttaaaa aaattaat
//
LOCUS       DATRSD010000103         4712 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRSD010000103 DATRSD010000000
VERSION     DATRSD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.104"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..217)
                     /locus_tag="VBD85_001789"
     CDS             complement(<1..217)
                     /locus_tag="VBD85_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7134323.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYNSKEDFLRESILIIFDDITKILNND"
     gene            400..729
                     /locus_tag="VBD85_001790"
     CDS             400..729
                     /locus_tag="VBD85_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7134324.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQSTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLE"
     gene            complement(849..1993)
                     /locus_tag="VBD85_001791"
     CDS             complement(join(849..1759,1759..1993))
                     /locus_tag="VBD85_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO7134325.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKEENYFNAIK"
     gene            2126..2263
                     /locus_tag="VBD85_001792"
     CDS             2126..2263
                     /locus_tag="VBD85_001792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134326.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2273..2528
                     /gene="mobC"
                     /locus_tag="VBD85_001793"
                     /pseudo
     CDS             2273..2528
                     /gene="mobC"
                     /locus_tag="VBD85_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2503..2997)
                     /locus_tag="VBD85_001794"
                     /pseudo
     CDS             complement(2503..2997)
                     /locus_tag="VBD85_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3191..4399)
                     /locus_tag="VBD85_001795"
     CDS             complement(3191..4399)
                     /locus_tag="VBD85_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7134327.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFKASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
BASE COUNT         1526 a          742 c          888 g         1556 t
ORIGIN      
        1 aatcattatt taaaattttt gtgatatcat caaagattat taaaatagac tccctaagga
       61 agtcttcttt tgaattgtaa tgattataga aagatcttct actaagtcga gctttttttg
      121 ctaaatcgct aactttaatt tgctcaaagc tcctcatctt taacaattct gagaaagctt
      181 ttaaaaattt taaattagtg ttatctgaac ttaccaatat actatcacct ttatataaaa
      241 tcttaaagtt ataaatctta tcgaatgtga ttatacaata caaattcaaa taatagttca
      301 catttatcga gataatgtaa aacagtaaaa ctttaaaaaa agtaaaaata gataacttta
      361 ttggttaatc tatttttact tttgaaaagg agagaagtta tgcttaggaa gtataagcaa
      421 agaaattata atgtcgcaca tacgaaacag cgtttttcga ttaagaaatt caaatttggt
      481 gtagcatcgg tattaattgg attgacattt ctaggaatga gtagtcattc tgttttggcg
      541 gatgagtcaa gcattatgag tactgaggta ccacaaacag ctggtgtaaa accggaagtt
      601 actgttacac agtctacttc agatgtaaca agtcttgcga cagatacgtc aacttcagca
      661 gtgttatcaa cagatgcatc tcattctaat cctacagaat cagcggagca tgctgtagga
      721 ttagaatgag atgcagcggt tccagtgaca cacgaaaaag aagcaaataa aacattgcga
      781 gttacttctt cttccacatc tgaattcaca gagtggacta atgtcaaata agtagacaga
      841 aaaccgtgtt attttattgc gttaaaataa ttttcttctt tctgattagg ggttagtcct
      901 agattagccg tatgtgggat gtaattgtta taaaaattct caatgtattc aaagcagtct
      961 aattgaacct gtttgatatt ttgataatgt tttcggttga tttgtctatg ctttaaatac
     1021 ttgaaaaatg cttcagttac ggcattatca taaggatatc caggtttaga aaaagaatgc
     1081 atgatattgt tgtcatcaat tatttttcta aattccctgg ccttaaattg tgacccttgg
     1141 tcggaatgaa agagaagtgt tccttcaatc tttcttttat taagagctaa ttctagagtg
     1201 ttacatgcta actttgcatc catacggtga ctcaatttcc aagcaataca ttttctagaa
     1261 tagagatcaa gtattgcgca gagatagaca tatttcttat atccaataga aatataggtg
     1321 aagtcagtag accatacttg attaggtttg tcaggataaa atttctggtt gagtaagttt
     1381 tgaggatacg ttttttgagt ctttttaaga gctgtttttg gcttaacggt agccatttta
     1441 gggagcgcca tattcttcat cagatggtaa attcttcctt cagagatttt tgtgtcgtag
     1501 tctctttgaa gaatgacttt gatagacttc acaccaagtc ttttgtttgc tttggtataa
     1561 atctcaagta tttgtttttt ataaatttga ttatccaatt ctctttttga gggcttatgt
     1621 tttagaaatt tatagtaagt ggagcgattg acacgtaaaa cacgacacaa ggttgttgtg
     1681 gcgtgttcaa agcgtaaccg atagacagcg aggagtctta ctttaagttt tgcatgaata
     1741 tggcacttgc tttttttagg ataaggttct cctcttcaag ctgcgcatta cgtttttgta
     1801 actcttgtat ttgcttggca gtaagtacag aattatcttc gagtttgaat tgagagtact
     1861 gtttgatcca ttttgcaagc gcagaagtgg ataccctata gtctttacag agttcagact
     1921 gagtttttcc agtttgataa aggttaacaa gagattgttt gaattcttca tcatagcgtt
     1981 taaatgttga cataggttgt cctttcgttt ttgtgtcttt taaaacagat tgtaacacac
     2041 aaagttttgt ctacttttat agtatacatc caccctttgg caataccaaa agtttgagca
     2101 gattagttga gatgtgcagg aagttttgat tatcgcaaga gagaatcatc aagtgactca
     2161 tgaacacgtg tcaatcttat tgacctgcgt tcaagaattg attgtggaag tcaatcaaac
     2221 acagccactc agtcgtgagt ttcgtgaaaa gtatatgggt taggaggctg caattgttta
     2281 tcgttatcgt accaatctca aaaaagtctt tctaacagat tcagaattga atcaattaaa
     2341 tgagcggatt gctaagagca attgtcaaaa tttctcagtc tatgctagaa gagttcttct
     2401 taatcccaat atgtcatttg tcacgattaa cacggattgg aaataacatc aatcaaattg
     2461 cgcgtgctat caaccaaagc catctaattt ctcaggctca gtatcacttt catcgagaag
     2521 cacgttaagc ccaacagcag gggggttgct ctgattcggt taagtaataa gcaaaatcct
     2581 caccaatttt cccagtaata agaggcgttg ttgaggtata gggattacct gtgccatagt
     2641 cgatgatggt cacaaaatga ccattcccca taaaagggcc aaccaaaact tcacctgtga
     2701 ccgttttttg atgtcaacac cagtattttg gatgtacccc ttaacatgcc ctttggtatc
     2761 tgcaacagag ataatatgac caaaggaaga atcaccgatg actttaaccg taatcttgct
     2821 atcgcctttt tgattagccg ctaatatttg attagcaatc agggtgcgac caagagcaac
     2881 tgttgaagac gacaaggtat gatgtttttc ctgagccaat ttaacagtct cggtgctatc
     2941 gaggacataa gacctgaagg caccagaggc agaaattgat ttaataattt tatccataac
     3001 ctatgttata gcacaaagag agagtttgta tggactggat tagctgaaaa ttttagacta
     3061 aaaagtagtg ccagtggatt ttgaaaggta acaaaagagt cttccgttta aacgagaagg
     3121 ctctttttag tcgtttcaga tgaaggcaaa aggacgatga aataattccg taccttcatg
     3181 gattgctatg ttactggcat gaggtctcac gatatttagt aagatattcg tttgaagata
     3241 ttcccacgta ttttttaaag gttttaagaa aatgtgtggt gtcgtaaaaa tgtaataatt
     3301 tcgctacttc cccaatcggt acccctcttt gcagtagtaa ttgtccctcc ttaatttttg
     3361 cctttagaat ataactttgc aaggaaatgt cagagtattt tttaaaaacc gagcgtaagt
     3421 gggaatctga gatatatagg tagttggcaa tatcagatac tttgagtttg gagtagaggt
     3481 ggttgtccac ataatggaga atactattgt acatttgctg cttgtcagag atgctcttat
     3541 tggttaagga ttctgaaaaa tcaataagag ctgcacagcg aattcttgaa acatcaataa
     3601 gatcaggagc ctcttcgttt aaagccatat agtgctttac cagcgcataa cttttagcca
     3661 catcagtatt ttcctcgaaa cttaattcta gtaattttgt taagtaaaca acagttaagt
     3721 tcttttcagc tcttagggca gggattgaag atgaggtaac actggatgat gggaggcgat
     3781 taatttcttg ctttaacagt tgagtgttac ccagcttaac gagatcaata atgtgattga
     3841 gatggtttaa aacagtgggt aactgaaaag agtttttctt agtatgtttt aggtgaagaa
     3901 caatatcagg atccgcaaca atctgttctg actcttctaa taaatgattg atgacttgtt
     3961 ggcaactagc cttaaactgt gtttggaaaa aggcatcgat agacacaaga agactacgta
     4021 tactggtagt aggaaaacaa gggacaagct ttatatagga taagatttct tttttactac
     4081 gatgagaaaa ttgttctaga aagcgactgg ataactgttc ttgcctattg atatcagggc
     4141 tatagggata aaatggtcca atagcaataa gatattgaca gacaggaaaa attaagaatg
     4201 attcttcaaa aagaccctca taaacagtga tatcttggtt ataagggata gctaaatgtt
     4261 ttaaaaactg atagtaaggc aacagatagt cttcgttacc atataactga acgagttcct
     4321 tgtctcgtga catgactgaa ataggtagtt gagatatggt atgcaatgtt tgaacatgtt
     4381 taaaatcgaa tgtaaccatt tgatagaccg ccttcattat catttctaga atttttcttt
     4441 aggtttgtaa agactacaaa ataaaatgat gaaaacaact atcttgtgga tacactaaaa
     4501 agacacgcta attagcaaac tctctcttca tcatctctca ccattattat actactattt
     4561 atatgacaaa taaaggtgat tttgttaaaa atataacttt gaaaatccac atatattttt
     4621 aatcttccgt ctgaaaaaaa taaataaaaa tagtaaaaat aaacacgaat ttaaaataag
     4681 caaatttttt aagaaaatct gtgctaaact tt
//
LOCUS       DATRSD010000104         4688 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRSD010000104 DATRSD010000000
VERSION     DATRSD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.105"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            42..686
                     /locus_tag="VBD85_001796"
     CDS             42..686
                     /locus_tag="VBD85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO7134328.1"
                     /translation="MPLLIHSLILSVLPFLMWLTLVNRDKPLYKTIWSILLGLQLITI
                     YTWFFWAKLPLSESLPLYHCRIGMFVVLLARPGILKDYFALLGVVGGVLAMIHPDFYP
                     YQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLTLLINMSLIFINLLT
                     GGNYGFMMKTPILGITNPFLNLFIVTTLLSFLVLFVKQIFQKVRMRASLKTEES"
     gene            865..2403
                     /locus_tag="VBD85_001797"
     CDS             865..2403
                     /locus_tag="VBD85_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7134329.1"
                     /translation="MNKSFNTKLGLVTVAVLSGIVLTSQLPVNAKAETPVMAASAQQG
                     FRFVATVVDSQTHVLPGKLVTLSEVTSGQPKIIASVKTNDAGQAIFNNLPIKRNLSVS
                     VDGQVKGYTIRTDVAGSSKAASFTATGVGTNEPTYSKKTIDITVRDQNAEPVSGRTVT
                     LKTQAGREIASLVSGDNGLTRFTDRLLDGTFYQYFVDGKKIGDIVPGESRSAYVNVAP
                     KKDYFTFTVTALDKDGLVVKGKEVTLTDITDGKAVALASLKTNDNGQAIFTNLPLSRN
                     ISVSIDGKSKGYTLRTDVAGSHKAAAFYVDGKGTKAPQFSAEAAVVTVYDANGNTIAN
                     QEVTLTNSNGTIVAKGLTEKDGKARFANKLMAGTLYNIFVNGIEMPKTALVGSDVSVF
                     LTDKQIKKEKPVTPDKLDKEMNQEKEKELLDTPKPVPPTSKPTAPIKNKKDDSPKKFT
                     KSQSSSLKSSVKAIGAKPKAMTALAKKLPKTGDQAISILTLVGFIVTGLAIMLGFLKK
                     SRSK"
     gene            2580..4124
                     /locus_tag="VBD85_001798"
     CDS             2580..4124
                     /locus_tag="VBD85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO7134330.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            4210..4590
                     /locus_tag="VBD85_001799"
     CDS             4210..4590
                     /locus_tag="VBD85_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7134331.1"
                     /translation="MGIFSGLMGNASQMDTDKVENQLSDILISDEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALATAVLK"
BASE COUNT         1524 a          720 c          930 g         1514 t
ORIGIN      
        1 tgatagaatt cttttctaat atcagaacag agattccgca gatgccttta cttatccata
       61 gtttgatttt atctgtctta ccttttctga tgtggctgac tttggttaat agagataagc
      121 ctttgtataa aactatttgg agtatccttt taggacttca gttaattacg atttatactt
      181 ggtttttctg ggcaaaattg cctttatctg aaagtcttcc cctttaccat tgtcgaatag
      241 gcatgtttgt cgttctcttg gcgaggcctg gaatattaaa ggattatttc gcattgttag
      301 gagtagtagg tggagtattg gcaatgattc acccagactt ttacccttac cagtttctac
      361 atgtgactaa tattttcttc tttattggac attttgcttt atttgtcctt agtttactac
      421 atctgatgac acagtccaat ttggacaaat taaatccaaa attgattata caactaacgc
      481 ttctcattaa tatgtctttg atttttatta acctcttgac tggggggaat tatggcttta
      541 tgatgaagac accaatacta ggaataacaa atcccttttt aaaccttttt atagtgacaa
      601 ctctattaag ttttctcgtc ctatttgtta aacaaatttt ccaaaaagtg agaatgagag
      661 ctagtcttaa aacagaagaa tcttaattat tttaatatta ttatattatc tagctattaa
      721 aaaataaata gctggataat gtttttcatt tttagctatt tttatgattg accgctaatt
      781 attaatctgc taagatatag tacgtaataa aaacttaacc ggttaattat ttttgaaata
      841 tattttttct ggaggaaaat agtaatgaat aaatcattca ataccaaatt aggtttggta
      901 actgtagcgg tattatcagg tattgtatta acgtctcaat tacctgtgaa cgctaaagct
      961 gagacaccag taatggcagc aagtgcacaa caaggtttcc gttttgtagc aaccgttgta
     1021 gatagtcaga cgcatgtctt gccgggaaaa ttggtaactt tgtcagaggt gacaagtggt
     1081 cagcctaaaa ttattgcaag tgtgaaaacg aatgatgccg gacaagctat ttttaataat
     1141 ctccctataa aacgtaacct tagtgtgtct gttgatggac aagtaaaagg atatactatt
     1201 cgaactgacg tagcaggttc tagtaaagca gctagtttta cagcaacagg agtagggaca
     1261 aatgagccaa cgtattcaaa aaaaaccata gacatcacag tgagagacca gaatgctgaa
     1321 cctgtttcag gcaggacagt taccttaaaa acacaagctg gtcgtgagat tgctagcttg
     1381 gtttctggtg acaatggatt aactcgcttt actgatcgtt tactagatgg aacattctac
     1441 caatattttg ttgatgggaa aaaaattggt gatattgtac ctggagaatc aagaagtgca
     1501 tatgttaatg ttgcacctaa aaaagattat tttaccttta cagtcactgc tttagataaa
     1561 gatggacttg ttgttaaggg aaaagaagta acgttgactg atattactga tggtaaagca
     1621 gtagcgcttg cttctttaaa aacgaatgat aatggacaag ctatctttac caacctacca
     1681 ttatcaagaa atatcagtgt ttcaattgat ggaaaatcaa agggctatac tcttcgaact
     1741 gatgtagcag gctctcataa agcggctgca ttctatgtag atggtaaagg taccaaagca
     1801 cctcaattta gtgcagaagc agcggttgta actgtttacg atgctaatgg aaatactata
     1861 gcaaaccaag aagtgacatt aactaattca aatgggacaa ttgttgcaaa aggtttgact
     1921 gaaaaagatg gcaaagcaag gttcgccaat aaattaatgg caggaacgtt atacaatatt
     1981 tttgtaaatg gtattgagat gcctaaaact gcccttgttg gtagtgatgt cagtgttttt
     2041 ctaacagata aacaaattaa gaaagaaaaa cctgttactc ctgataaatt ggataaagaa
     2101 atgaaccaag agaaggaaaa ggagctctta gatactccaa aaccagtacc acctacttcg
     2161 aaaccaacag caccaattaa aaataaaaag gacgattccc ctaagaagtt cacgaaaagt
     2221 caatcttcta gtttgaagtc aagtgttaag gctataggtg ctaagccaaa agctatgaca
     2281 gccttagcta aaaaattgcc taaaactggt gatcaagcca ttagtatttt aactttggtt
     2341 ggttttattg taacagggtt agcgattatg ttaggcttcc ttaaaaaatc acgatcaaaa
     2401 taatttattt tagaagcttg ggtctttaga cacaggcttc tcttctttct tcggaaatag
     2461 attgaacttg aagaatgttg ttaaattcgc tataataata aagttataga caagattatt
     2521 gtttcagttg agactactga aaataatact aagaaatgga taaaagctaa ggattgaata
     2581 tgacattaca agacgaaatc aaaaaacgcc gtacttttgc catcatctct cacccggatg
     2641 ctggtaagac gactattact gagcaattat tatattttgg tggtgaaatt agagaagcag
     2701 ggacagtaaa agggaaaaaa tcaggtactt ttgcaaagtc cgactggatg gatattgaaa
     2761 agcaacgggg tatctctgtt acttcatctg ttatgcaatt tgattacgcg ggtaaacgtg
     2821 ttaatatttt ggatacacct ggacatgagg atttctcgga agatacatac cggaccttaa
     2881 tggctgtgga tgcagctgtc atggttgtag attcggctaa aggtattgag gctcaaacta
     2941 aaaaactttt tgaagttgtt aagcatcgta atattccggt ttttacgttt attaataaat
     3001 tggaccgtga tggacgtgag ccattggatt tgcttgaaga gttagaagaa gtactaggta
     3061 ttgcttctta tccaatgaat tggccgattg gtatggggaa atcatttgaa gggctttatg
     3121 atttacataa taaacgttta gaactctata agggagacga gcgttttgca agtattgaag
     3181 acggtgatca gctgtttgcc aataatccat tttatgaaca agtcaaagaa gatattgaac
     3241 ttcttcaaga agcaggtaat gacttttcag aacaagctat tcttgatggt gacctaactc
     3301 cagtattctt tggctctgcc cttacgaact ttggagttca gacttttctt gatactttct
     3361 tggagtttgc tccagaacct catggtcaca agactacaga aggtaatgtt attgacccct
     3421 tggctaaaga tttttcaggt tttgtcttta aaattcaagc taacatggac ccacgtcatc
     3481 gcgaccgtat tgcttttgtt cgtattgttt ctggtgaatt tgagcgcggt atgggagtta
     3541 atttgacacg tactggtaaa ggtgctaagc tctccaatgt tactcaattt atggcagaat
     3601 cacgtgaaaa tgtgacgaat gctgttgctg gggatattat cggggtttat gatacaggaa
     3661 cttatcaagt tggggatacc ttaacagttg ggaaaaataa atttgaattt gaaccattac
     3721 caacttttac cccagaatta tttatgaaag tatcagcaaa aaatgttatg aagcagaaat
     3781 cattccacaa aggtattgag caattagtac aagaaggagc aatccagctt tataagaatt
     3841 atcagacagg cgaatacatg ctgggtgctg tgggtcaact tcagtttgaa gtttttaaac
     3901 atcgtatgga aggtgaatac aatgctgaag ttgttatgac accaatgggt aaaaagactg
     3961 tacgttggat taactcagac gatttagacg aacgcatgtc ttcaagccga aatattttag
     4021 caaaagatcg ttttgaccaa ccagttttcc tatttgaaaa tgattttgca cttcgctggt
     4081 ttgctgataa gtatcctgat gtgaagcttg aagaaaaaat gtaaaattta aagtcactca
     4141 aatttgagtg actttttaag ttttttagac taaaatagtt ataatacata gaaaagattg
     4201 gagattaaca tgggaatttt ttcgggatta atgggaaatg cttcacaaat ggataccgat
     4261 aaagtcgaga atcagttatc tgatattctg atttcagatg aacaggttga tttagcttat
     4321 accttaattc gtgatttgat tgtttttact aattatcgtt taattttagt cgataaacag
     4381 ggggtgacag gtaaaaaggt ttcttataat tctatacctt atgcttctat atcacgtttt
     4441 acagtggaaa ctagtggtca ttttgattta gatgctgagt taaagatttg gatatcatca
     4501 gcaatagaac cagcagaagt acttcagttt aagaacgacc gtaacatagt gagtatacag
     4561 aaagctttgg cgacagctgt tttaaaataa acaatttagc agtagtgatt tttatcactg
     4621 cttttatttt gaagtatagt aggcctcaag catatatttt gaaaacgctg tatttttttg
     4681 ataagata
//
LOCUS       DATRSD010000105         4575 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRSD010000105 DATRSD010000000
VERSION     DATRSD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.106"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            61..285
                     /locus_tag="VBD85_001800"
     CDS             61..285
                     /locus_tag="VBD85_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO7134332.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            363..2522
                     /gene="nrdE"
                     /locus_tag="VBD85_001801"
     CDS             363..2522
                     /gene="nrdE"
                     /locus_tag="VBD85_001801"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7134333.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFANHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDGSEVGANQCESCVI"
     gene            2725..3684
                     /gene="nrdF"
                     /locus_tag="VBD85_001802"
     CDS             2725..3684
                     /gene="nrdF"
                     /locus_tag="VBD85_001802"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7134334.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3769..3867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3925..4488
                     /gene="thiT"
                     /locus_tag="VBD85_001803"
     CDS             3925..4488
                     /gene="thiT"
                     /locus_tag="VBD85_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO7134335.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
BASE COUNT         1502 a          814 c          765 g         1494 t
ORIGIN      
        1 acttgctttt tccattcttt ttttatatgc tattaccatg aaaaaaatta ggaggacaac
       61 atggcagcta ttactgtttt ttcgaaaaac aactgtatgc aatgtaaaat gactaagaaa
      121 tttcttgacc agcacggcgc tgattttgaa gaaattaata ttgatgaaaa accagaaaaa
      181 attgaatatg tgaaaaatct cggcttttct gcggctccag tgattgaagc tggtaatgtt
      241 gttttttcag gtttccaacc aagcaaacta aaagaacttg tttaagcaaa ttagagtctg
      301 ggaataaagc aattaattgc tttattccca ttttgtattg ttaagttaag aggataaatt
      361 caatgagttt aaaaaatatt ggcgatgtat cttactttcg cctaaataat gaaattaacc
      421 gtcctgttaa tggacaaatc ccactccata aagataaaga agccttaact gcttttttca
      481 aagaaaacgt acagcctaat tcaaaagctt ttgactcaat cactgataaa attgcttacc
      541 tccttaagta tgattatctt gaagaagctt tcctcaataa atatcgtcct gaatttattg
      601 aagaattatc taccaaactc ttcgataaaa agtttcgttt taaatccttc atggcagcct
      661 ataagtttta tcagcagtat gcattaaaaa caaatgatgg agaatactat ttagaaagta
      721 ttgaagatcg cgttctcttt aacgctcttt attttgcaga cggtgatgaa gaactagcaa
      781 ctgacttagc tctagaaatg atttcacaac gctatcaacc tgcaacacca agttttttaa
      841 atgctggtcg aagtcgtcgc ggagaattag tctcatgttt cttaatccaa gtaactgatg
      901 atatgaatgc tattggtcgt tcgattaatt cagctcttca attatctcgt attggtggtg
      961 gggttggaat atcacttagt aaccttcgtg aggctggagc tcctatcaaa ggttttgctg
     1021 gtgctgcttc tggagtcgtt ccagtcatga aattatttga agacagtttt tcttactcca
     1081 accaacttgg acaacgccaa ggtgctggag ttgtttacct tgacgttttc catccagata
     1141 ttatttcatt cttatcaact aaaaaagaaa atgctgatga aaaagtacgt gttaaaacat
     1201 tgtcattagg gattactgta cccgataaat tctatgaact agcacgtaac aatcaagaaa
     1261 tgtacttgtt tagtccttat agtatcgaac gtgaatatgg tgttcctttc agctatattg
     1321 atattaccga aaaatacgat gaattagtcg ctaaccctaa tatcactaaa acaaaaatca
     1381 atgctcgtga tttagaaaca gagatttcta aacttcaaca agaatcaggt tatccttaca
     1441 tcatcaatat tgacactgct aatcgtacta accctgttga tggaaaaatt atcatgtcaa
     1501 atctttgctc tgagattttg caagtacaaa aaccaagtct tatcaatgat gctcaagaat
     1561 accttgaaat gggaactgat atttcttgta accttggctc aactaatgtt ttaaatatga
     1621 tgacatctcc tgattttgga aaatcaatta aaacaatgac tcgtgcttta actttcgtta
     1681 ctgactcatc aaatattgaa gctgttccaa ctattaaaaa cggaaatgct caagcacata
     1741 catttggact tggagcaatg ggactccact cctatttggc aaaaaatcat atcgaatatg
     1801 gtagtccaga atcaattgaa tttactgata tttatttcat gctaatgaac tactggacct
     1861 tagtagaatc aaacaatatt gcccgcgaac gtcaaacaac atttgtaggt tttgaaaaat
     1921 caaaatacgc tgacggtaca tactttgata aatatgtatc tggaaaattt gttccacaat
     1981 ctgacaaagt aaaatcccta tttgctaatc attttatccc cgaagctaaa gattgggaaa
     2041 accttagata cgcagttatg aaagacggac tatatcatca aaatcggctt gcagttgctc
     2101 cgaatggttc tatttcgtat atcaacgatt gttcagcatc tatccaccct attacacaac
     2161 gtattgaaga gcgtcaagag aaaaaaatcg ggaaaattta ttatccagct aatggcttag
     2221 ctacagatac tatcccttat tacacttctg cctatgatat ggatatgcgt aaagtaatag
     2281 acgtctatgc cgcagcaact gagcacgtag accaaggtct ctcaatgaca ctctttttac
     2341 gaagcgaatt accaaaggag ttgtacgagt ggaaaacaga aagtaagcag actactcgcg
     2401 acctttcaat cctacgcaac tatgctttca acaaaggtgt gaaatcaatc tactatatcc
     2461 gtacttttac agatgatggt agcgaagtcg gagctaatca atgcgaaagt tgtgtcatct
     2521 aacgaagaaa ctctacttaa aacatcagaa actagactga gataaactat ctcagtttca
     2581 tgatgttatc ttattttcta aggtattttc atctttattt ttctaaaaat cgataatact
     2641 tgaaatattg ctagatcaga attctatatt ctactcttct tccatcatca ttatataaaa
     2701 acctatttac cattggagaa aaccatgaca acttactacg aagctataaa ctggaacgaa
     2761 attgaagatg ttattgataa atcaacttgg gaaaaactaa ccgaacaatt ttggctcgat
     2821 acacgtatcc ctttatcaaa tgacttagac gattggcgca aactttctgc tcaagaaaaa
     2881 gatcttgttg gcaaggtttt tggaggctta accctacttg ataccatgca atcagaaact
     2941 ggtgttgaag ctattcgtgc cgatgttcgc acgcctcacg aagaagctgt cttaaacaat
     3001 attcaattca tggaatctgt tcacgctaaa tcttattctt caattttctc aactttaaat
     3061 actaaatcag aaattgaaga aattttcgag tggactaata ataatgagtt ccttcaagaa
     3121 aaagcacgta ttatcaatga catttatgct aatggaaatg cccttcaaaa aaaggtggct
     3181 tccacctacc tcgaaacttt ccttttttat tctggctttt tcacacctct ttactatttg
     3241 ggaaataata agttagcaaa tgttgctgaa atcattaaat taattattcg tgatgaatct
     3301 gtacatggta cttatatcgg ttacaaattc cagcttggtt ttaacgaatt accagaagat
     3361 gagcaagaga attttcgtga ttggatgtat gacctccttt atcagctgta tgaaaacgaa
     3421 gaaaaataca ccaagacact ttatgatggc gtaggatgga ctgaagaagt tatgaccttt
     3481 ttacgctaca atgctaataa agctcttatg aatttaggac aagatccttt attcccagat
     3541 acagcaaatg atgtcaaccc aattgttatg aatggtattt caacaggaac atcaaaccat
     3601 gacttcttct ctcaagtagg taatggttac ctacttggta gcgttgaagc tatgcatgat
     3661 gatgactata actatggatt ataagaaaaa gacactgatt tagtgtcttt ttctagttaa
     3721 atacaaattt cttgacaagc aaaccctttc aagatagaat gttagagtaa atacacaagg
     3781 gagtgccttg agctgagatt gcagatatgc aaaatcctct aacctgatct cgttaggacg
     3841 agcgtaggaa ttgtggtttg aattggttaa acatgacaaa tgacaaaaga atctcctttg
     3901 ctgtataaaa aaaggagatt ttttatgtca aaaaataaca acactacctg tcttattgaa
     3961 actgctattt ttgcagcgct tgctatggct ttatcaatga ttcctgattt tgcgtcatgg
     4021 ttcactccct cttttggagc aattcctctg atactttttg cattacgtcg tggtacaaaa
     4081 tacggtctat ttgctggctt aatttggggc ttattgcact ttgttctttc caaagtatat
     4141 taccttagtt tatcccaagt ttttatagaa tacatcttag catttataag tatgggactt
     4201 gcaggcgtat tttctgctaa atttaaagat gcacttagca gcagctctaa aactaaagct
     4261 ttatcattag cattatctgg agcaatatta gcaacatttg ttcgttatgt ttggcattat
     4321 atcgctggtg ttattttttg ggctagttat gcaccaaaag gtatgtctgc tacattatac
     4381 tcattaagcg tcaatggtac tgctggtttg ttaactctat tttttgtagt cattagtatt
     4441 attattttag ttatcagcta ccctagcttc tttttaccta agaaatagaa aaaataaaag
     4501 gactgatttt cagagaatca atccttttat tttacactaa tataagtttt ttgataaaaa
     4561 atctagctac tacta
//
LOCUS       DATRSD010000106         4570 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRSD010000106 DATRSD010000000
VERSION     DATRSD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.107"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..1342
                     /locus_tag="VBD85_001804"
     rRNA            <1..1342
                     /locus_tag="VBD85_001804"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1397..1469
                     /locus_tag="VBD85_001805"
     tRNA            1397..1469
                     /locus_tag="VBD85_001805"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1430..1432,aa:Ala,seq:tgc)
     gene            1624..4526
                     /locus_tag="VBD85_001806"
     rRNA            1624..4526
                     /locus_tag="VBD85_001806"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1273 a          936 c         1338 g         1023 t
ORIGIN      
        1 aaaaggagca attgcttcac tgtgagatgg acctgcgttg tattagctag ttggtgaggt
       61 aaaggctcac caaggcgacg atacatagcc gacctgagag ggtgatcggc cacactggga
      121 ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttcgg caatggacgg
      181 aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa agctctgttg
      241 ttagagaaga acgttggtag gagtggaaaa tctaccaagt gacggtaact aaccagaaag
      301 ggacggctaa ctacgtgcca gcagccgcgg taatacgtag gtcccgagcg ttgtccggat
      361 ttattgggcg taaagcgagc gcaggcggtt ctttaagtct gaagttaaag gcagtggctt
      421 aaccattgta cgctttggaa actggaggac ttgagtgcag aaggggagag tggaattcca
      481 tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg gtggcgaaag cggctctctg
      541 gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt
      601 agtccacgcc gtaaacgatg agtgctaggt gttaggccct ttccggggct tagtgccgca
      661 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat
      721 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc
      781 ttaccaggtc ttgacatcct tctgaccggc ctagagatag gctttctctt cggagcagaa
      841 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc
      901 aacgagcgca acccctattg ttagttgcca tcattaagtt gggcactcta gcgagactgc
      961 cggtaataaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg
     1021 gctacacacg tgctacaatg gttggtacaa cgagtcgcaa gccggtgacg gcaagctaat
     1081 ctcttaaagc caatctcagt tcggattgta ggctgcaact cgcctacatg aagtcggaat
     1141 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg
     1201 cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt ttaggagcca
     1261 gccgcctaag gtgggataga tgattggggt gaagtcgtaa caaggtagcc gtatcggaag
     1321 gtgcggctgg atcacctcct ttctaaggat aaggaaacct gccatttgcg tcttgtttag
     1381 ttttgagagg tcttgtgggg ccttagctca gctgggagag cgcctgcttt gcacgcagga
     1441 ggtcagcggt tcgatcccgc taggctccat tgaatcgaaa ggttcaaatt gttcattgaa
     1501 aattgaatat ctatatcaaa ttccacgatc tagaaataga ttgtagaaag taacaagaaa
     1561 ataaaccgaa aacgctgtga atatttaatg agttttctag ttttaaagaa actaggttaa
     1621 taaggttaag ttaataaggg cgcacggtgg atgccttggc actagaagcc gaagaaggac
     1681 gtgactaacg acgaaatgct ttggggagct gtaagtaagc gctgatccag agatgtccga
     1741 atgggggaac ccactagtta atgactagta tccatatctg ttaaggatat gagaaggaag
     1801 acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaaagc gattgcctta
     1861 gtagcggcga gcgaaacggc aggagggcaa accgaagtgt ttacacttcg gggttgtagg
     1921 actgcgatat aggattaatc attatagaag aattacctgg gaaggtaagc caaagagagt
     1981 aacagcctcg tatttaaaat gatagattaa ccttagcagt atcctgagta cggcgagaca
     2041 cgcgaaatct cgtcggaatc tgggaggacc atctcccaac cctaaatact ctctagtgac
     2101 cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg gagtgaaata
     2161 gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt
     2221 ttgtagaatg aaccggcgag ttacgatatg atgcgaggtt aagttgaaga gacggagccg
     2281 tagggaaacc gagtcttaat agggcgtcat agtatcatgt tgtagacccg aaaccatgtg
     2341 acctacccat gagcaggttg aaggtgaggt aaaactcact ggaggaccga accagggcac
     2401 gttgaaaagt gcttggatga cttgtgggta gcggagaaat tccaaacgaa cttggagata
     2461 gctggttctc tccgaaatag ctttagggct agcgtcgatg ttaagtctct tggaggtaga
     2521 gcactgtttg ggtgaggggt ccatcccgga ttaccaatct cagataaact ccgaatgcca
     2581 acgagatata atcggcagtc agactgcgag tgctaagatc cgtagtcgaa agggaaacag
     2641 cccagaccac cagctaaggt cccaaaatat atgttaagtg gaaaaggatg tggggttgca
     2701 cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg taatagctca
     2761 ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacatattac cgaagctgtg
     2821 gatgacacaa aagtgtcatg gtaggagagc gttctatgtg tgaagaaggt gtaccgtgag
     2881 gagcgctgga acgcatagaa gtgagaatgc cggtatgagt agcgcaagac aggtgagaat
     2941 cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg gttagtcggg
     3001 acctaaggag agaccgaaag gtgtatccga tggacaacag gttgatattc ctgtactaga
     3061 gtatatagtg atggagggac gcagtaggct aactaaacca gacgattgga agtgtctggt
     3121 caaacagtga ggtgtgatat gagtcaaatg cttatatctt taacattgag ctgtgacgag
     3181 gagcgaagtt tagtagcgaa gttagtgatg tcacactgcc aagaaaagct tctagcgata
     3241 catatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga
     3301 gcgagagaac tctcgttaag gaactcggca aaatggcccc gtaacttcgg gagaaggggc
     3361 gctgatttta gatcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
     3421 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
     3481 gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac
     3541 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
     3601 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
     3661 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
     3721 gtatctgtac cacatgtaca ggataggtag gagcctatga taacgggacg ccagtttcgt
     3781 ttgaggcgtt gttgggatac tacccttgtg ttatggctac tctaacccag ataggcaatc
     3841 cctatcggag acagtgtctg acgggcagtt tgactggggc ggtcgcctcc taaaaggtaa
     3901 cggaggcgcc caaaggttcc ctcagactgg ttggaaatca gtcgtagagt gtaaaggtat
     3961 aagggagctt gactgcgaga gctacaactc gagcagggac gaaagtcggg cttagtgatc
     4021 cggtggttcc gtatggaagg gccatcgctc aacggataaa agctaccctg gggataacag
     4081 gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat gtcggctcgt
     4141 cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta aagcggcacg
     4201 cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa
     4261 tttgagagga tctgctccta gtacgagagg accagagtgg acttaccgct ggtgtaccag
     4321 ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa gggataagcg ctgaaagcat
     4381 ctaagtgcga agcccacctc aagatgagat ttcccatgat tttatatcag taagagccct
     4441 gagagatgat caggtagata ggttaggagt gtaattgtgg tgacacattt agcggactaa
     4501 tactaatagc tcgaggactt atccaaaaag agatattgac aacgttacgg tttcttgtta
     4561 gactatagat
//
LOCUS       DATRSD010000107         4548 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRSD010000107 DATRSD010000000
VERSION     DATRSD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.108"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            214..1065
                     /locus_tag="VBD85_001807"
     CDS             214..1065
                     /locus_tag="VBD85_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7134336.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQGMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            1058..2926
                     /locus_tag="VBD85_001808"
     CDS             1058..2926
                     /locus_tag="VBD85_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO7134337.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSILEKL
                     IETIAGVITPLIPALLGGGMLKVIGILLPMLGIASSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSLFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALSILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIAAIATIILTFIITWFLGIDE
                     GENEKSSINAQEHTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDAETMVTNEDDLLVIL"
     gene            2943..4370
                     /locus_tag="VBD85_001809"
     CDS             2943..4370
                     /locus_tag="VBD85_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO7134338.1"
                     /translation="MTVFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMTDKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIKIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPHLTTS
                     EWGWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
BASE COUNT         1463 a          677 c          904 g         1504 t
ORIGIN      
        1 gtttattgaa agcgcttgac aagattagta agaaatgata aaattaagac gttttcaggt
       61 gttattattt aaggtaagca agctgataac agataaaatt gcataataaa ggatgttatc
      121 acattttgtg agcagggacc tattcaacga cgactttttg ttgtgtcttg gatgggtcat
      181 tttttattgt gcgagtgaag gactaggact ttaatgatta taaaacgcgt attaaatcat
      241 aatgcagtca tatcagtaac acatcaagga ttagatgtgc tactgatggg gaaaggaatt
      301 gcatttaaga aaagaatagg tgataggatt aatagcgatg ctattgagaa gtcatttgtt
      361 ttaaaaaata gtgataacat gaaccgtttt acggagttat ttattactgt tccagaagaa
      421 gtagttgcat gttcagaacg aattatcaac ctaggtaaaa tcaaattggg taagaactta
      481 gatgaaattc tttatatcaa tttgacagat catattcatt cggctattga aagacatgaa
      541 caaggaatgg ttatccaaaa tccactaagg ttggaaatcc aacgttacta tcctgatgaa
      601 tactccattg ggatgaaggc tctggaactt ataaaggatg aattgggcat ttgtttaaca
      661 atcgatgagt cggcttttat tgctatgcac tttgtaaatg ctggattgga taatccgttt
      721 aatgaagcgc ataaaatcac tgagattgta tcttacattg aacagaaagt aaaaatagat
      781 tttcgaacag aactagatga aagttcaatt gattattatc gtttcatgac acataccaaa
      841 ttatttgctc aacgcgtttt atcaggaatg aaatacgaag atgatgatgc tgatttactt
      901 ttggtagtta agaaaaaata tccgagagaa tataaatgtg ttaaagagat cggcaacaat
      961 atggctatcc aataccaata tcaacttaat tcaagtgaac tcctatatct gacggtgcat
     1021 gttaaacgat tagttaaaaa tttaaaggag atatcagatg actaaatacc aagagactgc
     1081 taaagctatt ttagcagcag tagggggaga aaaaaatatt caacacgtaa cccactgtgt
     1141 gacacgatta cgattagttt tagacaatga tgaaattgtc aacgatcagg ttattaaaac
     1201 tattccgaat gttatcggtg ttatgcgtaa aaatgatcaa taccaaatta ttttgggaaa
     1261 tgatgtcaat aattattata atgctttttt ggccttaggt cattttgaaa atactaccag
     1321 agaattttca tcacaaaaaa agagttcaat acttgagaaa ttaattgaaa cgattgcagg
     1381 agtcattacg ccgcttatac ctgctcttct aggaggaggg atgctcaagg ttataggtat
     1441 tttgctccct atgctcggta tagcaagctc tagttctcaa acagtagctt ttatcaattt
     1501 ttttggtgat gccgcttatt attttatgcc aataatgatt gcttattcag ctgcgtcacg
     1561 atttaaagtt acacctgtat tagctgctac agtaggaggg attctcttac accctgcttt
     1621 tgtgacaatg gtagctgaag gaaaaccatt atctttattt ggagctccgg ttacactggc
     1681 tagttatggt tcttctgtta ttccaatctt aattatggtt tttcttatgc aatatattga
     1741 gagatggatt aataaaattg ttcctagtgt aatgaagagt tttttacaac cgactttgat
     1801 tattctcatt tctggctttt tagctttagt ggtcgtgggg ccactggggg taattattgg
     1861 taaaggattg tctagtgcaa tgctttcgat ttaccatgtg gcgccatggt tagctttatc
     1921 tattcttggt gctattatgc cattggttgt tatgacagga atgcactggg catttgctcc
     1981 aatattttta gctgcttctg ttgcgacacc agatgttttg attttaccag caatgttagc
     2041 ttctaattta gctcaaggtg cggcttctct tgctgttgca gttaaggcta aacaaaaaca
     2101 aacacgtcag gtggcctttg cagcaggttt atcagcacta ctagctggta tcacagaacc
     2161 tgcactttat ggtgtcacac tgaaattcaa gaagccttta tacgctgcaa tgatatcagg
     2221 tggtttagta ggagcttata ttggattagt taatattgct tcctatacat ttgtagtacc
     2281 atctattatt ggtttgccac aatatatcaa tccacagggg ggcaataatt tcagtaatgc
     2341 tgttattgca gcaatagcta ctattatttt gactttcatt attacatggt tcttgggaat
     2401 tgatgaagga gaaaacgaaa aaagtagtat taatgctcaa gagcacacac atattagaag
     2461 tggtttatca aaaaaagaga cattgtattc acctatggta ggcaatgtat tacctttatc
     2521 taaagtaccc gatgaaacat tttcatctaa attactaggt gaaggtttag caatcactcc
     2581 tagtgtgggt gaagtttatg caccatttga tggtgaaatt ataagtttat ttcctacaaa
     2641 acatgccatt gccttaaagg atgataaggg agtcgaagtg ttaatccata ttggaataga
     2701 tactgtagaa ctaaatggag aaggttttga acaattggtc aaagttggcg attttgttaa
     2761 acgaggacag ttacttttgc gaatggatat tgattttatt agttcaaagg ggtattctct
     2821 tattagtccg gttgtagtta caaattcaat tgaccaactt gaaattatcg ttaaagacgc
     2881 agagacaatg gttacaaatg aagatgattt acttgtaatt ctttaaaagg aaggatgata
     2941 atatgacggt ttttcctaaa cattttctat ggggaggtgc agttgctgcc aatcaggtag
     3001 agggtgcatt tagaacagat ggcaaaggtt tatctgtaca agatgtactt ccaaatggtg
     3061 gtttaggcga tttcaccgcc aaacctactc cagacaattt gaagctagaa gcaattgatt
     3121 tttatcataa ctataaaaat gatataaaac tgtttgcaga gatgggcttt aaagttttta
     3181 gaacctctat tgcctggtct cgaatttttc caaatggtga tgatagtgct ccaaacgaag
     3241 ctggcttaca attttatgat aatttatttg atgagttgct taaatataat atcgagccct
     3301 tggttacctt atctcattat gaaacaccgc ttcatcttgc aaaaacttat aatggttggg
     3361 ctgatagacg tttgatagca ttttttgaga aatttgctca aacagtcatg gagcgttata
     3421 aagataaggt gaaatattgg ttgactttta atgaagtgaa ttctatttta catatgcctt
     3481 tcaccagtgg tgctattatg accgacaaat cacaactaag tcctcaggag ctttatcaag
     3541 ctattcatca tgaattagta gcatcggcta gagtgacaaa attagggcgt tctatcaatc
     3601 ctaattttaa aattggctgt atgattctgg ctatgccagc ttatcctatg acatctgatc
     3661 caagagatgt cctggctgca agacaatttg aacaacataa tctgctattt tcagatatac
     3721 atgtcagagg taaatatccc acttatattc agtcctattt caaaaataat ggtattaaaa
     3781 ttaaatttga agaaggagat gaggaagtat tggcacaaaa tacagtagat tttctatcgt
     3841 ttagttacta tatgagcgtg acacaagctt atgactttga aaattatcaa agtggtcagg
     3901 gaaatatcct tggaggccta actaatcctc atttaacaac ttcagaatgg ggatggcaaa
     3961 tcgatcccat cgggctacgc ctagtcttaa atcagtatta tgaacgttat cagattccgt
     4021 tgtttattgt agaaaatgga ttgggagcaa aagatcaatt gatagaaaca ctagacggtg
     4081 attatactgt cgaagatgat tatcgtattg attatatgaa tcaacattta gttcaagttg
     4141 ctaaagctat tgaagatggt gttgaaataa tgggatatac atcttggggg tgtattgatt
     4201 gtgtatccat gtctacagct caactaagta aacgttatgg tctaatatat gttgatcgaa
     4261 atgatgatgg aacagggagt ctacaacgtt acaagaagaa gtcatttggt tggtaccaaa
     4321 aagtgattaa gacaaatggc caatcattgt ttgaacatca taacagataa catgttaaac
     4381 aactagtctc tgtttcaatt taatataaaa aggagtatcc ataggttact cctttttatt
     4441 ataatttatg aaggttaata tcttctgatt taagtgctag atacaataaa gttgcatcct
     4501 taaagtttct tggattgagt tgcgttgatt cataaatctt atttaacc
//
LOCUS       DATRSD010000108         4460 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRSD010000108 DATRSD010000000
VERSION     DATRSD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.109"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..535
                     /locus_tag="VBD85_001810"
     CDS             <1..535
                     /locus_tag="VBD85_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEO7134339.1"
                     /translation="LYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLV
                     FLYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALL
                     IFHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSI
                     VFQKVILDLLSKKRRRR"
     gene            535..1560
                     /gene="neuB"
                     /locus_tag="VBD85_001811"
     CDS             535..1560
                     /gene="neuB"
                     /locus_tag="VBD85_001811"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO7134340.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKETTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            1637..2791
                     /gene="neuC"
                     /locus_tag="VBD85_001812"
     CDS             1637..2791
                     /gene="neuC"
                     /locus_tag="VBD85_001812"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO7134341.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAVTKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKGNASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            2788..3417
                     /locus_tag="VBD85_001813"
     CDS             2788..3417
                     /locus_tag="VBD85_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7134342.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDCIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            3428..>4460
                     /gene="neuA"
                     /locus_tag="VBD85_001814"
     CDS             3428..>4460
                     /gene="neuA"
                     /locus_tag="VBD85_001814"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO7134343.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDVAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRFTT
                     IAYTLFRETV"
BASE COUNT         1463 a          679 c          858 g         1460 t
ORIGIN      
        1 tctctattta ggagttttct taacattagg ttatttgact gtttttcctg agttagctca
       61 aataatggga ggagacaagt atagtagtag tgttcaattt atttctttaa ttattgtcag
      121 ctacttttta gtctttctct atacatttcc tgtaaatatc caattcttct atgctaatac
      181 gacatggatt cctattggga cattattagc tgctggtgta aattggttac taaaccttgt
      241 attaattcct cattatgcag cctatggcgc tgctatggct acaataatat cttatttagc
      301 cttgttgatt tttcatcata ttgtttcgaa agttaaatac cattattcag atgtctcagt
      361 taggcagtac atcattttaa gtggtatagt atttagttat gctatgttaa tgaacatgtt
      421 cctagataat atcgttatta gatggagttt aggaataatt attcttatag tatatagcat
      481 tgtttttcaa aaagtcattt tagacttatt gagtaaaaaa agaaggagaa gataatggtt
      541 tatattattg cagagattgg ttgcaatcat aatggagata ttaatcttgc gaaaaaaatg
      601 gtagatgttg cggtgtcttg tggtgttgat gctgttaaat ttcagacttt taaagctgag
      661 aaacttattt ctaaatttgc tcccaaagct gaatatcaaa aagaaactac aggaacagca
      721 gacagtcaac ttgagatgac gaaacgttta gagttaagct ttgaagaata cttagaaatg
      781 cgtgattatg caatttcaaa aggtgtggag accttttcaa caccttttga tgaagagtca
      841 ttagagttct taatttctac agatatgcca atttacaaaa ttccatcagg agaaatcact
      901 aatttacctt acttagaaaa gattggcaag caacaaaaga aagttattct ttcgacgggt
      961 atggcggtaa tggaagagat ccatcaagcg gtgaatattt tacgtcagaa tggtacaact
     1021 gacatttcta ttttacattg tacaacagag tacccaacac cttacccctc tctaaattta
     1081 aacgttattc atactttgaa agatgaattt aaagatttaa cgataggtta ttcggatcat
     1141 tcaattggat cagaagtacc tatcgcagca gcagcaatgg gtgcagaagt tattgaaaaa
     1201 cactttactt tagatactaa tatggaaggt ccggatcata aagccagtgc aacacctgat
     1261 attttagctg ctttagttaa aggggttcgc attgttgaac aagccttagg tagatttgaa
     1321 aaaatcccag atccagtaga agaaaaaaat aagattgttg ctcgtaaatc agtcgttgct
     1381 ttaaaaccaa ttaaaaaagg cgatatttat tcaatagaaa atattacggt gaagcgccca
     1441 ggtaatggta tttctcctat gaactggtat gacatcttgg gacaagaagc gcaagatgat
     1501 ttcgaagagg atgaagttat tcgtgattca cgctttgaaa atcaattgcc cgagttataa
     1561 taacacctaa ttataatcgg caacagatgt attacacggc taagattatg taattactat
     1621 atatagaagg gatacgatga aaaaaatttg tttagtaacc ggatcgcgtg ctgaatacgg
     1681 aataatgaag ccattgattc aaagattatc aaaagataaa gaagtcaact tacaaattat
     1741 tgcgacagca atgcatctgg aagaaaagta cggctatact tatcgtcaaa ttgaagaaga
     1801 cggttttgat attgcttata aagttccctt acatctttat gatactgaca gaagaactgt
     1861 atctactgca atggcgcatt tacaactagg attgaccaaa atttttgaca aggaagacta
     1921 tgatctagtc atcattttag gggatcgtta cgaaatgtta ccagttgtga atgtagcgtt
     1981 gatttataat gtcccagtat gccaccttca tggaggggag acctcattag gcaattttga
     2041 tgagtatatt cgccatgcag ttactaagat gagtcaccta cacttagtct ctacagagga
     2101 ctttcgtcaa cgtgtgattc agatgggaga acaacctcaa tttgtaatta acacaggagc
     2161 tctcggagtg gaaaatgctc tatcaatccc ttctctaacc aaagaagcaa tagaaaagca
     2221 attaggtatt gtactagagg aatcctattt tgttgttctc tatcatcctg ttacttttga
     2281 acaaggaaaa agtgctggag aacagatgaa agcggtacta tcagcccttt ctaaatttgg
     2341 tgtccagtgt ctatttatag gatccaattc agatactggt tctgatgata ttgcaaaggc
     2401 tatcaatact tatttgataa atcatgaaaa tagttattgc tttgcttctt tatcaaccca
     2461 actttatcat tccttaattc gtcattcact aggactgatt ggtaattcgt cttctggttt
     2521 aatagaagtt ccttctttga tgaaacctac tcttaatatt ggtgatcgcc aaaaaggtag
     2581 gctacatgga gaaagtgtag taagtgtacc agtcgaaacc tcctctgtat tggagggatt
     2641 atcaaaattg aatgaagtga ccaactttga caatccctac tacaaaggga atgcttcatc
     2701 tattgcatat gaagcaataa agctttattt gaaagatgaa ccttcaattt cccaaccatt
     2761 ttatgattta aaggagaata atctaaaatg aaaaaaaagt tggcattttt aggcgcagga
     2821 agtcatgctg atgcgattgc tccggttatt gatcctcttg tgtatgattt cgtaggtttt
     2881 tttgatgata aagatattac ggagcatgat ggttatcctg ttcttggaaa actttatgat
     2941 gtgctacctt accttgaaga tggctcaata gatgcagtat ttattacaat aggtgataat
     3001 gctaaaagga aagaactatt tgaatatgta gcaaaggatt attatgactg tattattaac
     3061 atcattagtc ccaatgcttt agtattgaca ccagatagta tttgtgggcg tggtatcttt
     3121 attggttttg gggcttttat aggttctaaa gtgaagctgt ttgataacaa cgttgttaat
     3181 acaggagcgc tcattgaaca tcatactgtt gtagaatcac actgtaatat agcacctaat
     3241 gctaccataa atggtctttg ttatattaga gaagaagttt atgtaggtag tgccagtgtt
     3301 attattcaaa ccttggatat ttcatcgtgt acgacaattg gcgcaggagc tgttgttgta
     3361 aaagatatta tagaacctgg aacctatgta ggggttccag ccaaaaaaat aaaataaagg
     3421 atatgttatg aagccaattt gtattattcc tgcgcgatca gggtcaaaag gattaccaga
     3481 taagaatatg ttattcctag caggtaaacc catgattttc catactatag atgttgcaat
     3541 tgaatctgga atgtttgata agaaagatat ttttgttagt acagattcag aattgtacag
     3601 agagatttgt ttagaacgcg gtatttcagt ggtgatgaga aaaccggaac tttcaactga
     3661 tcaggcaact tcgtatgata tgttaaaaga ttttttatct gactatgaag ataatcagga
     3721 gtttgtgtta cttcaagtaa cctctccact aagaaaatca tggcatataa aggaagcaat
     3781 ggagtattat tcttcacatg atgttgacaa tgttgtaagt ttttctgaag ttgagaaaca
     3841 ccctggtctg tttacgacat tgtctgataa aggctatgct atagatatgg tgggagcaga
     3901 taaaggttat cgtcgccaag atttacaacc tttatactat ccgaacggcg ctatttttat
     3961 ttctaataaa gaaacttact taagggaaaa aagctttttc acctctagga catatgctta
     4021 tcaaatggca aaggaatttt cattagatgt tgatacgaga gatgatttta tccacgtcat
     4081 cggtcacctc tttttcgatt atgccattag ggaaaaagag aataaagttt tttataaaga
     4141 aggctatagt cgtttgttca atagagaagc ttcaaagata attttaggtg attcaaaaac
     4201 gatatctatc tcactagaaa attaccataa ttattcccaa ggtggtgtaa cattagcaac
     4261 gatgttggaa aacttaccta actttttgac agctaatgta acagaggctt ttgtttctat
     4321 aggggtaaat gatctcatta caggttatag tgtggaagaa atattcagca atttccaaaa
     4381 actctactca ttattagctg agaataaaat aaagatgaga tttacaacta ttgcttacac
     4441 cctttttaga gaaactgtca
//
LOCUS       DATRSD010000109         4459 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRSD010000109 DATRSD010000000
VERSION     DATRSD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.110"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..525)
                     /locus_tag="VBD85_001815"
     CDS             complement(<1..525)
                     /locus_tag="VBD85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEO7134344.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHD"
     gene            complement(665..2092)
                     /locus_tag="VBD85_001816"
     CDS             complement(665..2092)
                     /locus_tag="VBD85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7134345.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGKRIGKLAGMRPIMLELGGKDAGVVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(2242..3975)
                     /gene="ptsP"
                     /locus_tag="VBD85_001817"
     CDS             complement(2242..3975)
                     /gene="ptsP"
                     /locus_tag="VBD85_001817"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO7134346.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3980..4243)
                     /locus_tag="VBD85_001818"
     CDS             complement(3980..4243)
                     /locus_tag="VBD85_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO7134347.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         1333 a          902 c          730 g         1494 t
ORIGIN      
        1 atcatgttcc tttagttgag ttcctggagc tatattttct aaaatagcct taaatccaga
       61 tatattatga gctaaaagct catctaattc aaaaggtttc ttattttgat caagtaagta
      121 ggttctcaaa aggcgacttg atttttgaaa catacttcct ttttcttttt gatataagat
      181 ttgataaatg gataattttt taacatttgc aaagttagaa tctgctaatt gaggacgtag
      241 aataatgtat tgattgtcat aaaatttttt atgctcaaag cttttttgat tccactttaa
      301 aatatcagcg cttagacttt tgtcatatcc tttaggttca agaaaaagaa cacgttcagt
      361 tttctgtctt ttcaccactt ctaccgtttt ctcggctatg cgaatttctt tttcaacaac
      421 agcttgttga agttttattt ctttatgttt caaaaacgaa aaaccttgcc aagcaagaat
      481 cgctataata attagactaa aaatacctat aattaatttc ttcatataat gactcttttg
      541 ttatttttta tgacaattat atcacaaaaa taaaaccact ttagaagatt atataattct
      601 ttatttttaa tgtaaaagag gtttataaaa cgactcaact gacaatagtt gagtcgtttt
      661 acgtctattt catatcaaaa actatcgatt ttacatttgt cattgcttca attgaatatc
      721 tgataccttg aacacctgca ccagatcctt tgagtcctaa gaatgggaaa ttatcaggac
      781 cacgtccagt cttattgtta atgtgaactg taccaacttc aagtttattt gctatatcaa
      841 atgctttttg gaaatcacgt gtaaagactg atgattgtaa tccaaaatca gatttgttgg
      901 caatagcaac agcttcttct gcatccttga cacgaataat tgggagaatt ggtccaaaag
      961 gctcttccca agctagtttc atatctaaag taacatgatc aaacaatcct ggagttaata
     1021 gacgaccatc acgtttaaat tcattcaatt ctttcgcacc tttttgacgt gcatctacta
     1081 ctaagctttc aataaagtcg gctgaattat catcaataac cggtgtcacc gttgcattat
     1141 caaatggatc acctactgat aattttgcta cattttcaga tattttttct gccaattcat
     1201 ctgcaacttc ttcaacgaca agtacacgct taattgccgt acagcgttgt ccggagtaat
     1261 cataagcacc tgcaacgatt tgtttagcag cgttgtcaag gtcagcatca gctaagacga
     1321 cacctgcatc cttaccaccc aactcaagca taattggacg cattcctgct aacttaccaa
     1381 tacgttttcc aactggcgtt gatcctgtaa agttaataaa attaacttct tcatgctcaa
     1441 caatgtaatc tccaatctca gaaccgcgtc ctgtaatagt attaaagaca cctgctggaa
     1501 gacctgcttc tgcaaaagct tttgctaaaa caagtcctga gactgaacct tgtgttggtg
     1561 gtttaaacat cacaacgttt ccaccaatta gagctggcgc aatttttgat cctgagaggt
     1621 taacagggta attataaggt gcgattgcta aaacgatacc tactggttga cgacgaataa
     1681 cagccaactt atgacctgtt gaagcatcca tttttccacc ttcgtcagct gaagttgata
     1741 aacgaattcc ttcttctgct gcataacgaa taagatcagc tgtccttaca acctcagtta
     1801 ctgaagcatt gtaagcttta gaaatttctt ttgctaaaac agtagcaatt tcttctttat
     1861 cacgttcaat aatgtctgcg gctttatgaa ggtattgtgc acgttcatat actgtcaaag
     1921 cagcccaggc tggtaaagcc tcacgacccg ctttcatagc atgatcaact tcttctcgag
     1981 tcatcgctgg cacgaatccc aatgaagaat catcaattgg tgacaaaatc tcaatctgat
     2041 taacagatga tttccattcg ccattgacat aattttgata ttcttttgtc aaagtaaatc
     2101 tccttttaca ttgtaatagg tttattctat cactttatct atttatttca attatctgac
     2161 atatttttct tagaaactta acaatatcca aactttcaat aaaaaagtag agatttttta
     2221 atccctactt ttttattatt ctcagtcaaa gtcaacgtat tctttttgaa gttcgataac
     2281 ttcttccata gttgaacact ctgaaagagc acgattagcg tattcttcca ttttagctgt
     2341 gtccaatttt ttcattaagc tacgtgtacg aagtactgag gttgctgaca ttgagaattc
     2401 atccaatccc ataccaacaa gaagtggaac cgcagtttga tcaccggcca tctcaccaca
     2461 catacctgcc catttacctt cagcatgagc agctttgata acgttgttaa tcaaacgaag
     2521 gattgatgga ttgtatggtt gatagaggta tgaaacttgc tcattcatac ggtccgcagc
     2581 cattgtgtat tggataagat cattagtacc aattgagaag aaatcaactt cttttgcaaa
     2641 ttggtctgca agcatagctg ccgctgggat ttcaatcata ataccaactt caatgccttc
     2701 agctactgct acaccgtcag ctaacaagtt cgctttctct tcttcaaata tagctttagc
     2761 agcacggaat tctttaagca aagcaaccat tgggaacatg atgcgtagct gtccatgaac
     2821 tgaagcacga agaagtgctc ggatttgtgt gcggaacatt gcatcaccag tttcagaaat
     2881 tgaaatacgc aatgcacgga atcctaagaa tgggttcatc tcttttggaa gatcaaagta
     2941 aggaagctct ttatctccac cgatatccat tgtacgaaca acaactggtt taccattcat
     3001 accctcaaga actgctttat atgcttcata ttgctcatct tctgttggga aatcttgtga
     3061 atccatatat aagaactctg tacggtaaag accaactgct tctgctccat tttcgttaac
     3121 accctcaaca tctttaggtg taccgatatt agcagctaat tcaaaatgtt taccatcagc
     3181 agtaactgtt tgtgcatcct taagaagtgc ccattcagct ttttgtttgg cataagcttc
     3241 acctgctgcc ttgaaggctg aaatttgagc ttcagttggt tcaatgataa cttcaccagt
     3301 aataccatta acagcaatca actgaccatc ttgaacacgt tcagtaatat catttgtacc
     3361 aagaacagct gcgatttcga gtgtacgtgc cataatagct gagtgacttg tacgccctcc
     3421 aatatttgtt acaaaagctt taacaaattg tttatttaat tgagctgtat cagaaggtgt
     3481 caaatcgtgt gcaataacga ttgattcttc attgatagtt gctgggtttg gtaatttaac
     3541 acccaaaaga tgtgctaaga cacgtttggc aacatcacgg atatctgctg cacgttcttg
     3601 catgtatggg ttatcttcca taccttcaaa gatagtgatg aacatatcag tcacttcttt
     3661 aagacctgtt tctgcattta cttgttttgc acgaatagtc tctttaattt gattaatcat
     3721 ttctgggtca gaaagaacca ttaaatgagc atcaaataca gctgcggctt cttcgcctaa
     3781 gctttctact gccttctcac ggataacaga aagctcgtct tgtgaagctt ggagtgcaac
     3841 atcaaggcga gcttcttctg cgtttgtatc ttcgactgtt acagtctcaa atgacaaatc
     3901 cggttggaca agtagatatg ctttagcaac tgcaacacca tcagaggctg cgattccttt
     3961 aagcatttct gtcattttct tatgccaatc cttcttttgt cattgtttct tcgatagctg
     4021 caattgcatc atctgcatct gcaccttctg ctgaaatagt aacatcagca ccttgaccaa
     4081 caccaagact cataacaccc atgattgatt taaggtttac tgctttacct ttgtaatcaa
     4141 gcgtgatgtc tgaagcaaat ttgctagctg tttggacaag caaagtagct ggacgtgcgt
     4201 gaatacctgt ttctgcaaca atgtggaaat cttttgaagc catattatgg ttctcctttt
     4261 gtcatttgta aatatttgag tcacctattg gtaaccctta caagttaaaa gtataacata
     4321 ttgcttttta tttttcaaga ttaaatgtct caaacagaaa aaaacttgaa tgttttccac
     4381 agcttttcat attaaaaatg agagtcttag aatttaaaaa tagaattcta aaaatgattt
     4441 ttttatgttc ttctgaaag
//
LOCUS       DATRSD010000110         4428 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRSD010000110 DATRSD010000000
VERSION     DATRSD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.111"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..937
                     /gene="dnaN"
                     /locus_tag="VBD85_001819"
     CDS             <1..937
                     /gene="dnaN"
                     /locus_tag="VBD85_001819"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7134348.1"
                     /translation="TSNESRLYLLEAGFFINIISSLPDVTLEFTEIEQHQIVLTSGKS
                     EITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTGVHLVI
                     SQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETVEVFFS
                     DSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYLISNAT
                     QNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESLKAVKS
                     ETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            995..1888
                     /locus_tag="VBD85_001820"
     CDS             995..1888
                     /locus_tag="VBD85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7134349.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGQIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQQIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGYTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            1898..2095
                     /locus_tag="VBD85_001821"
     CDS             1898..2095
                     /locus_tag="VBD85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7134350.1"
                     /translation="MYQVGSLVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2297..2413
                     /locus_tag="VBD85_001822"
     CDS             2297..2413
                     /locus_tag="VBD85_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134351.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLANTNEVKVERIYEKNLDL"
     gene            2657..3772
                     /gene="ychF"
                     /locus_tag="VBD85_001823"
     CDS             2657..3772
                     /gene="ychF"
                     /locus_tag="VBD85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO7134352.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            3856..>4428
                     /locus_tag="VBD85_001824"
     CDS             3856..>4428
                     /locus_tag="VBD85_001824"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO7134353.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAIDRIVKNLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVKYYLQTNDFQKTMQKYNGLK"
BASE COUNT         1538 a          624 c          827 g         1439 t
ORIGIN      
        1 tactagtaac gaatccaggc tctatttatt agaagctggt ttctttatta atattatttc
       61 aagtttacca gatgtaactt tagaatttac agaaattgaa caacatcaaa ttgttcttac
      121 tagtggaaaa tcagagatta ctttgaaagg taaggatgtc gatcaatacc ctcgtctgca
      181 ggaaatgaca acagatactc cattaacatt agaaactaaa ctgttaaaat caattattaa
      241 tgaaactgct tttgctgcta gccaacaaga aagccgtcca atcttaacag gtgttcattt
      301 ggttatcagt caaaataaat actttaaggc tgttgcgaca gattcacacc gtatgagtca
      361 acgcactttc caattagaga aatcggctaa taattttgat ttggttgttc caagtaaatc
      421 ccttcgagaa ttttcggctg tttttacaga tgatattgaa actgtagagg ttttcttctc
      481 agatagtcaa atgctattta gaagtgaaaa tatcagcttc tatacacgtt tgcttgaagg
      541 aaactaccct gatactgatc gcctcctaac taatcagttt gaaaccgaaa ttatctttaa
      601 tacaaatgct ttacgccatg ctatggaacg tgcttattta atttcgaatg caactcagaa
      661 cggtactgtt cgtttagaaa ttcaaaatga aacagtctca gctcatgtaa actctccaga
      721 agttggtaaa gttaatgagg aattggatac tgttagcctt aaaggtgata gtttaaatat
      781 tagttttaat ccaacttacc taattgaatc tttaaaagca gtaaaaagcg aaacagttac
      841 gattcgattt atttctccag tacgtccatt tactttgaca cctggtgaag atactgaaga
      901 tttcatacaa ttaataactc ctgttcgtac taactaaata agaaaactac ccaagaataa
      961 cttgggtagt tttcttattc taagatatgc tataatgatg agtaatatga ctctatatat
     1021 aatagctaac ccccatgctg gtaataaaaa tgcctccact attgttggtc aaattcagga
     1081 gctttatcat actgaagata tttctgtgtt ctatacagaa cagaaagatg atgaaaaaaa
     1141 acaagtcatt aatatactaa ggtcttttaa agaaagtgat catctaatga ttataggagg
     1201 agatggtacc ttatcaaaag taatgactta tctcccccaa catattccgt gcgcttatta
     1261 tcctgttggt tcgggaaatg attttgctag agctttgaaa attcctaacc tcaaagaaac
     1321 ccttaccgct attcagacag aaagattaaa ggaaattaat tgctttattt atgataaagg
     1381 gctgattttg aatagtttag atttaggttt tgcagcctat gtcgtttgga aagcatctaa
     1441 ctctaaaata aaaaatattt tgaatagata tcgtctagga aaaataactt acattgttat
     1501 agctatcaaa tcattattac attcttctaa agtacaagta ttagtggaag gagagacagg
     1561 acagcagata aaactaaatg acctttattt tttcgcatta gcaaataaca cttattttgg
     1621 aggtggcatt actatttggc ctaaagcaag tgctttaact gcagagttag atatggttta
     1681 cgctaaaggg tatacttttc taaaacgatt aagtatttta ttatctttgg tttttaaaag
     1741 gcatactacc tctaaaagta taaaacacca gactttcaaa gctatgaccg tatattttcc
     1801 taaaaatagt ttgattgaaa tagatggcga aatagttgag ttagatcaga ttagtttaaa
     1861 atgtcaaaaa aggtatttat atatgtaagg agaaaagatg tatcaagttg gatcacttgt
     1921 tgaaatgaaa aaacctcatg cttgtgtaat taaagaaact ggtaaaaagg ctaatcaatg
     1981 gaaggtactg agagtaggag ctgatattaa aattcagtgt actaactgtc agcatgtgat
     2041 tatgatgagt cgttatgatt ttgagcgaaa attgaaaaaa gtactgcaac catgaaagtc
     2101 cttttaaacc aataattata gctatatttg caaccaatac tttcctaaat aatgctagta
     2161 tgccgttgga aaattagcta ttctaatgtt atcgaaagaa gaaaggtggc cattgacaat
     2221 gacgaaagtt gcagaacagt tgtttatttt tcggtaagtc atatcaaaat gggaaaatgg
     2281 ggataccaca ccagatttgg ataatttaat caggttagct gagatttttg acgtgagctt
     2341 agatgaacat gttttagcta acacaaatga agttaaagtt gaacgtattt atgaaaaaaa
     2401 tcttgatctg taaaaatata ataaaattta ttggttaatt agtagaaata ttattctatt
     2461 tctactaatt atcttagcta tattaacttt cttataagct ctaggtcttc cctttgtttc
     2521 ggagtggata atttaaatga gggtgaaaga gtggcataat gataagcaaa tctgaaattt
     2581 tcttaccatc ataccgcttt ttcttctatt ttttgatata atagtcttga ttgaaatttg
     2641 aatggagact cactaaatgg ctttaacagc aggtattgta ggcttaccaa atgttggtaa
     2701 atcaacttta tttaacgcaa ttacaaaagc aggagcagaa gctgctaatt atccttttgc
     2761 gactattgat cccaatgttg gtatggtaga ggtaccagat gagcgtctgc aaaaattgac
     2821 agaattgatt acgcctaaaa aaacagttcc gacaaccttt gaatttactg atattgcagg
     2881 tattgttaaa ggtgcttcga agggggaagg tctaggaaat aaatttttag ctaatattcg
     2941 tgaagtggat gctattgttc atgtggtacg tgcttttgat gatgaaaatg ttatgcgtga
     3001 acaaggtcgt gaggatgcct tcgttgatcc tatagcagat attgatacta ttaatcttga
     3061 attaatctta gctgatttag agtcaatcaa taaacgttat gcgcgtgttg aaaaaatggc
     3121 acgaactcaa aaagataaag aatcagtagc agagttcaat gttttacaaa agattaaacc
     3181 tgttttggaa gatgggaaat cagctcgtac cattgagttt acagaagagg aagcaaaagt
     3241 tgttaaaggt ctctttttat taacaactaa acctgtttta tatgttgcta atgttgatga
     3301 ggacaaagta gctgatcctg atgatatcga ctatgttaat caaattcgtg catttgcaga
     3361 aacagagagt gcagaagtag ttgtgatttc tgctcgagct gaagaagaaa tatctgaact
     3421 tgatgatgaa gataagttag aatttttaga agctattgga ttaacagaat ctggtgttga
     3481 taaattaaca cgagctgcct atcatctttt aggccttgga acttacttta ctgctggtga
     3541 aaaagaggtt cgcgcttgga cgtttaagcg tggtataaaa gctccacaag ctgctggtat
     3601 tatccattca gattttgaaa gaggttttat tcgtgcagta acgatgtcat atgatgacct
     3661 tattcaatat ggatctgaaa aagcagtaaa agaagctggc cggttacgtg aagaaggaaa
     3721 agagtatatt gttcaagatg gcgatatcat ggaattccgt ttcaatgtct agtagtaaat
     3781 aatcaatttg ttaactttta ggttaaggat attgttatta tccttaacca tttttgcgtt
     3841 ttaatggaga agataatggt aaaaatgatt gttggattag gaaatccagg aagtaaatat
     3901 aatgatacaa aacataatat tggatttatg gctattgaca gaattgtaaa aaaccttgac
     3961 gtcaacttta cagaagataa aaattttaaa gcagagatag gtagtgattt tatcaacggt
     4021 gaaaaaattt attttatcaa accaactact tttatgaata acagtggtat tgcggtaaaa
     4081 gccctgctta cttactacaa catttctatt aaggatatga ttatcatata tgatgattta
     4141 gacatggaag tcggtaaaat acgttttcgt caaaaaggat ctgctggggg acataatggt
     4201 atcaagtcta ttattgctca tttaggtact caagaatttg atcgtattaa agtaggtata
     4261 ggacgtccga atggacgtat gacagttatt aatcatgttc tagggaagtt tgataaaaat
     4321 gatgaaatta tgatatcaaa tactcttgat aaggttgaca atgctgtcaa gtattattta
     4381 cagacgaatg attttcaaaa aacaatgcag aaatacaacg gtttaaaa
//
LOCUS       DATRSD010000111         4378 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRSD010000111 DATRSD010000000
VERSION     DATRSD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.112"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            137..1876
                     /locus_tag="VBD85_001825"
     CDS             137..1876
                     /locus_tag="VBD85_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7134354.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRRNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHKNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     LGTCAIVNIPIKN"
     gene            1888..2622
                     /gene="ltdR"
                     /locus_tag="VBD85_001826"
     CDS             1888..2622
                     /gene="ltdR"
                     /locus_tag="VBD85_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO7134355.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEHDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVTSGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2778..4262
                     /locus_tag="VBD85_001827"
     CDS             2778..4262
                     /locus_tag="VBD85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO7134356.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
BASE COUNT         1372 a          759 c          766 g         1481 t
ORIGIN      
        1 tttcataata gttgttgatg gatatcccct taattgtctt gagaatactt gatagagaga
       61 cattttaaat gagtaatgaa atgaatcatt ttatacctat gttataacgg actaattaat
      121 actttggaga gatttaatga cactcttttt aattatgatg gagcgagcgg ggttaatcat
      181 attattagcc tatgcctttg tgcatatccc ctttataaaa caaactttga agcaacccga
      241 attaaaaaaa caccagtata ttctgcttat cttatttagc ttatttgcta ttatttctaa
      301 ttttacaggg gtagaaattc agtcagattt atctatcatt ccacaaactc ttaatcatat
      361 agcagatcaa tcatcggttg ccaacacacg tgttttaacc attggtgtat caggtctaat
      421 cggtggccca atagtcggta ttatagttgg tctcttatct gtattcgtta ggtacttgca
      481 aggaggatta gcccctcata tttatgtcat ttcttcctta ctaattggtt tatgctctgg
      541 gttatcaggc aattatcttc gtagaaatta taataaaatt cgtgttttag atgcaatggt
      601 tgttggtttc ggtatggaaa ttctacaaat gatctgtatc ctcattttta gtgttgactt
      661 taatcaggcg cttcgattag tatcttttat cagcatgcca atgattttaa gtaatacttt
      721 aggtttaggt atatttatat ccattatttc atctactcaa aaattagaag aacatgctaa
      781 agcatttcaa acccatcaag tacttgaatt agcaaattta acccttcctt accttagaaa
      841 gggactgaca acagaatctt gtcaaccagt agctgaaatt attcataaac atatggatgt
      901 gagtgctgtt agcctgacta gtcaatcagc cattttagcc tatgtaggag atggagctga
      961 ccatcatctt cctaatactc aaatattaac aaagcttgct aaaagagcaa tagatactgg
     1021 taaagttagt gtcgcaactg ataaaagtga aatcgaatgc gaccataaga attgcccact
     1081 tagttcagca attgttattc cattacacat ccacgatgtt atagtgggta ctttgaaatt
     1141 atatttttca gacgcacaac atatgactta cgttgaccgt caattagctg aaggattagg
     1201 caatatcttt tccactcagt tagctttggg acaagctgag gaagcaacaa ggttactaca
     1261 ggatgcggaa atgaagtctc tccaagcgca ggttaatccg cattttcttt ttaatgcact
     1321 caatactatt tatggtttaa ttcgcatgga tagcgaaaaa gcaagaaagc tcgtccaaga
     1381 ttttagcaag gttattcgtg ctaatcttca acgggcaaag caaaatttaa ttcctctcca
     1441 tgatgaattg gaacaggtta acgcctattt agcccttgaa gaagcaagat ttcctaacat
     1501 ggtggccttt aatttagaca accaaacaaa ttcagatgac aacttaatga ttcctccctt
     1561 tacattgcag gtcttgattg aaaactctta taaacacgct tttaagcata tcaataaaaa
     1621 taaccagcta aaggttacta ttgcaagaaa taatgatagg cttcatatca ttgtccagga
     1681 caatggtatt gggattccaa aagaaaaatt aataacgctt ggaaaaaaaa ctcaaatttc
     1741 aaaaaaaggt tctggtacag ctattgagaa tctcgttcgt cgcttgaata ttatttatga
     1801 tggacaagca agtttaaaat ttgaatcaaa tgatttagga acttgtgcta ttgttaacat
     1861 ccccattaaa aattagaaag gtattgtatg aaagttttag tagttgatga tgaaccagtt
     1921 gcacgtaacg aattaattta ccttcttaat aagtatgatt ctaacctcgt tatagcagag
     1981 gcgcatgata tggctactgc attagctatt ttacttagag aaacttttga tgtagcactg
     2041 ttagatatcc atctcagaga tgattctggg ttgcaattag cagagtatat caataaaatg
     2101 cccaaaccac cattattgat attcgcgact gcttatgatc aatatgctat tcaagctttt
     2161 gagcatgatg cgcgtgatta tttgttaaaa ccctatgagt ttgataggct aaagcaagct
     2221 atggatagag taaaaggagc gctaagtaca tctacaatta tagagagcgt aacttccggc
     2281 cctctcttca agcaacagta tccattgaca gtagaagatc gaatctatct ggtgtcggcg
     2341 gatgatatcc ttttgattga agctatgcaa ggaaaactga ttatacaaac acctgataaa
     2401 aattatgaaa ttgatggctc tctacaacaa tggcaagata aactaccatc atctcaattt
     2461 gtacgggtgc accgctctta cattgtgaat attaatgcta ttaaaacgat tgaaccttgg
     2521 tttaaccaaa cacttcagtt acacctttgt aataaaataa cagttcctgt tagcagagca
     2581 aatgtaaaac ccctaaaaca aatgttaggc atatctacct aactaataaa aagttattca
     2641 tcataaaact tagactattc atttctaaaa aacaaccatt catcttgaaa ataggaaatt
     2701 tcaatttttc aatgctatac taaagtcatg aaaacgctta ataaaattaa gcaaaaaaat
     2761 ttttaggagg cagtgatatg gtaacatttt taggaggggt agccctttta attgttggtt
     2821 atttcacgta cggtagatac attgaaaaga attttcaaat tgatgaaaat cgccaaacac
     2881 ctgcagaagc attaagggat ggatatgatt ttgttcctat gccaaaatgg aagaatggga
     2941 tgatcgagtt attgaacatc gcaggaaccg gacctatctt tggacctatt ttaggagcac
     3001 tctatggtcc agttgcttat atctggatag tcttaggatg tatttttgca ggggctgttc
     3061 atgattatat gattggaatg atttctctta gaaataatgg tgcatatctt cctgaattag
     3121 ctagtcgcta tcttggaaag tcaatgaagc atgtgatcaa cattttttca atgctattac
     3181 ttattttagt tgcaaccgta tttgtggtaa ctccagctaa tcttatccta agtattttac
     3241 cagctgggac actatcttta ccttggataa tcggtcttat tttcgtttat taccttattt
     3301 ctacagtctt acctattgat aaagcacttg gtaaagttta tcctgtattc tgcgtcatcc
     3361 taatggttag tacagcagca gttggcttcc gtttactaac tggtggcttt gatatgccaa
     3421 acttaacttt tgaaaccttt aaaaatatgc atccagctgg gcttgggata ttccctgctt
     3481 tattctttac catttcatgt ggagccatct ctggtttcca tgctacccaa gcacctatgg
     3541 tctcaagaac aactgttaat gagagagaag ggcgtttcac attctacggc atgatgattg
     3601 cagaaggagt aattgcgatg atttgggcag gtgcttcaat gagcttgttc aaaggtcaaa
     3661 atctctatga aatgattgct gctggaacac cttctgcagt cgtcaatcaa gtaatgttaa
     3721 tgttactcgg tagtgtaatt ggaactatcg ctatcattgg tgttattgtt ctccctgttt
     3781 catctggttt atcagctttc cgtagcttac gtactatcgt tgctgattat atccatgtca
     3841 aacaagatac tcttccaaaa atctttgctg taactatccc attatatgtt atttcatttg
     3901 tccttacaca cgttgatttt aatctcctat ggcgttactt taactgggca aatcaagtaa
     3961 ctgctgttat tggtttatta gttgctacaa gatatctcat cctaaaaaga cgaaattatt
     4021 gggtaacatt tgtaccagct atgttcatgt tgtatgcagt agttgtttat attttatctc
     4081 aaccaattgg atttaatatg ggcttaggca tcttaactta ttcacttgct ttagtgttaa
     4141 caggtattct tgttggactt ttctggaaat ctggtcaaaa acaattaaaa actgttcatc
     4201 ctgaagcatt cctgtttaac gatcatagac caattaatta ttacagtagt ttagattctt
     4261 aatattattt tgaagccatg ttattaagac ttacagttat cacaataacc tcgaatgaag
     4321 tatcattcga ggttattttt ctactaaaaa tctcagtaaa tatttctatt tgattaca
//
LOCUS       DATRSD010000112         4356 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRSD010000112 DATRSD010000000
VERSION     DATRSD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.113"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            129..1258
                     /locus_tag="VBD85_001828"
                     /pseudo
     CDS             129..1258
                     /locus_tag="VBD85_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1346..1678)
                     /locus_tag="VBD85_001829"
     CDS             complement(1346..1678)
                     /locus_tag="VBD85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134357.1"
                     /translation="MTNHFSIFMTIGGAFFLVGNIYLYVQLYKIIKIDASGRGFKHPK
                     FWAFFASSGNNSGGLLLYLLGRRSYPVNLTDNQQKLIVKCKKRFTVGLIFFVTGLIVI
                     IWSLAFMN"
     gene            complement(1689..2096)
                     /locus_tag="VBD85_001830"
     CDS             complement(1689..2096)
                     /locus_tag="VBD85_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134358.1"
                     /translation="MDIGKKLKSARKDNHLTQEKVSELIQVSRQTISNWENGKSYPDV
                     ISVIILSDLYHVSLDVLLKEDVEMIQHIEESTNIVKSNKKLMLAILLEIIIFLVLIVI
                     ASFFETNILLITGTFTLATLATAFLLYQIVKRI"
     gene            2352..2666
                     /locus_tag="VBD85_001831"
                     /pseudo
     CDS             2352..2666
                     /locus_tag="VBD85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3263..3527
                     /locus_tag="VBD85_001832"
                     /pseudo
     CDS             3263..3527
                     /locus_tag="VBD85_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3767..3937
                     /locus_tag="VBD85_001833"
     CDS             3767..3937
                     /locus_tag="VBD85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134359.1"
                     /translation="MAESANTGRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKEN
                     KVIEGGHPQVIG"
BASE COUNT         1583 a          662 c          734 g         1377 t
ORIGIN      
        1 taatcaatca aactagatgt aaatcattgg ataaaaaaac agaaccttcg gcactgctcc
       61 cctaaaatga gacaaaagaa aaacactcct gttgaaatct agtaaactag aaacaggagt
      121 gttttattat ggttaaaaaa gcatattcat gggaaacaaa gttagcctgc ttcaagatga
      181 agaaagcagg caagtctaac aaggttatta tggaaaatct aggtataaaa aatgatagtc
      241 aaatctatac ttggatgaaa tggtacgaaa atgaggaact tcatcgtttc catcaaggtg
      301 ttggcaagca atatacctat ggcaagggat tagaacagct gtctgaaata gaacggttac
      361 aattgcaagt gaacctctta aaaaagtatc gaagcttgat aaggaaatcg ataaagtaag
      421 tcttatcaag cttgtagaag acaataaaag gaactaaccg attgctgtca ttctagactg
      481 ttttgatgtt aagcggtcga cctattaccg ttggaaaaag gagtatgaga aaccgcaaaa
      541 aacagatgac gtcattgcat taattgaaca gctctgcatg gagaatcact ttatttatgg
      601 atatcgtacc attacacgat tgctcaaaaa gatccacggc ttgactgtta atgcaaagaa
      661 agtctatcgt atcatgaaaa ataatagttg gctttgtcgg acacgtacca agaaagttcc
      721 caatttgggt aaagcttatt atttaacaga taacaaattg agtcgggatt tccatgccga
      781 taagcctatg gaaaagcttg taacagatat tacctaccta aattttggta actgtaaatt
      841 gtatctctct tcaattatgg atctctataa tcgagagatt atagcatata ctatctccga
      901 ttgtcaggac acagattttg tactagacac tcttaatcag ttaaaattgc ctaaaggtgc
      961 aatcttacac agtgaccaag gctcagtgta tacttctaag gcttactatc aggcttgcac
     1021 taaaaaaggc attatccgat caatgtcccg aaaaggaaca cctgcagata acgcctgtat
     1081 tgaatggttt cactccgtcc taaagtctga aaccttttat ctccataata ggagaaaata
     1141 caacaaagat agtataacaa atattgttaa aaattacata acattttata atgaaactag
     1201 aattcaacag aaactaaacg atctgtctcc tgtacaatac agaagacaga tcgcataaaa
     1261 gggtttttct tatgtcccac tattggggtg cagtgccttc tctttggttc tgtttttaat
     1321 ctcaagaaaa ttatgatatt atgaattaat tcataaaagc gagactccaa atgataacga
     1381 taagtccagt tacaaaaaat ataagcccaa ctgtgaatct ttttttacac ttaacgatca
     1441 acttttgttg attatcagtt aaattgactg gatatgacct acggccgagt aaataaagaa
     1501 gtaaaccacc ggagttgttt ccagaacttg caaaaaaagc ccaaaattta ggatgtttga
     1561 atcccctacc tgatgcatca atttttatga ttttataaag ttgaacatac aaataaatat
     1621 taccaactaa gaaaaatgcg ccaccgatag tcataaaaat agagaaatga tttgtcatag
     1681 tattcacctt aaatcctttt aacaatttgg tacaaaagaa atgcagtagc aagtgttgcc
     1741 aaagtaaatg ttcctgtaat gagtaatatg ttggtttcaa aaaagctagc aataactatc
     1801 aaaactaaaa agatgataat ctctagaaga atggctaaca ttaatttttt attgctctta
     1861 acaatatttg tactttcttc aatatgttga atcatttcca catcctcctt caacaataca
     1921 tccaggctta catggtataa atcagataga ataataacac taatcacatc aggataggat
     1981 ttcccatttt cccaattgga gatagtttgt ctagagacct gaattagttc ggagactttt
     2041 tcttgcgtta agtgattatc ttttctagct gactttagtt tttttccaat atccactttt
     2101 acctccctta ataatttcat cttcccataa atttctaata ctgtccatat aaatattttt
     2161 acatttgaca ttaaccctgt caaaattctt tgacatcctt aaaaaacgag tagtctttat
     2221 tgaaaaatta aatgtttttt actaattata tcactattga aggtagtgta aaatatcttg
     2281 tgtaaacaca aaaaaggaat aaatcttgaa taatagagtt acgacacttc actagtaaga
     2341 gatttattcc catgactcag tttaccacag attacttaac ttcctagtac aaaaacaaga
     2401 tattgttgaa taattctttc gttccttttt agaaatagct atgaatgacc tcttgcaggt
     2461 tgaactaact atcagcatgc cttggatatg agccatatga aaaagaaggt tacaatacag
     2521 ttaacagccg taatggtact tattctcgat agtttgaaac caagtatagc ttagtcaaat
     2581 tgaacattcc aagagataga aatggcaact tttcatcagt ttttttgcca tcttatgcta
     2641 gacgtgatga tcacttggaa gaaattaaga caggtgtata aaatattctg gtcactccaa
     2701 aatcactaaa atctcaaaat tacttagttt ttaagttgat ttctaaaact caaatcttgt
     2761 taaatgaagg tttgttagct ttaaaaagtg cttggtagga ctcaaaatcc agtgtcctca
     2821 cggacgtgcc ggttcgaccc cggccgccgg tatactttaa aagacaaggt ttctaaacct
     2881 tgtctttttt gtattgtact ggtaattttg agaaaaagtt actgaaaatt acttatattt
     2941 gttgttcatt attgcaattg ttattgaagt tttaaaacaa gcagaaataa taaggaaatt
     3001 tttcaagagt tagtggagac tcaacattta aatgcaattc aaagaggact cctaaatgtc
     3061 atagattctg aagtaataaa agtgtttgtg aatgatagat aggtagtata aaataataag
     3121 atttttcagt gtatatgtta cgactaaaaa attaaactcg tgaaaatttg ttatactaat
     3181 attagtttta acatactggt aaataataat tatttttatt agatacttga catgggaagt
     3241 ttattttcta agaaaagaga gcatgatgaa aaaatatgta aaatggttaa ttcccatatc
     3301 tattattggg atgttactag caggctgtca aatgaatagt gaatctaaaa gtcagtcaaa
     3361 cgaagtaaaa aatagcaaac agtcagcagt aaagaaaggt aaaaaagtaa tgaaaaaaga
     3421 acaagtagct tatctcaaaa agcatgaaaa agaattaact gaatatgtca aaatccaaaa
     3481 tccagatatt agtgaaatta agtatgattg gaatagtgtg cgtatagtgg aagctagtaa
     3541 tggaacccct caaggcggag agcaactatt attaattttg ggttatgcaa atggcagtga
     3601 attaaccaat tttactctaa atttcacatt gggtgataat caaatgccta atattgactc
     3661 agttggctca gataatttat caactattga agattaataa taacaataag aaaggtatat
     3721 aatgggatga tggaatctta aggaatttga agatatattt tgaagtatgg cagaaagtgc
     3781 taatacagga cgatcgaatt cttttgtttt agaagaatta actgaatttc aaagagaaaa
     3841 gttaaaaaat ggggagtcag ttaagtttaa tttttctaaa gatgttaaga aagaaaataa
     3901 ggttatcgaa ggagggcacc cccaagtcat agggtaatct acctgcaaac tggataagtc
     3961 cttaaataca attgatgaaa aagtagaggt tcgcgttcct gatgtcaatg gcggttatca
     4021 ctctgagcat tgtgttacta attcctatca gaaaggttac tgaaagatga tgaagcagta
     4081 gctctataat cactaaaagc tcatcattgc ttagtgttta agttaacttc taaagctcga
     4141 accttgttaa atcaaggctt gtttactttt aaaagtgaca ttttattttt ctaaaaatga
     4201 ggttatcggg attcctcatt ctaagttact ttgtttatat gtacctttac ttaaggttta
     4261 ttttcattat ttttaattta atattttgga gtatgtacgc tccattatag tattttcgaa
     4321 gacatcttta aaagaactgt agaagttaaa atattt
//
LOCUS       DATRSD010000113         4310 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRSD010000113 DATRSD010000000
VERSION     DATRSD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.114"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(48..1610)
                     /gene="guaA"
                     /locus_tag="VBD85_001834"
     CDS             complement(48..1610)
                     /gene="guaA"
                     /locus_tag="VBD85_001834"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO7134360.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1823..2521
                     /locus_tag="VBD85_001835"
     CDS             1823..2521
                     /locus_tag="VBD85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7134361.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            2635..3969
                     /gene="trmFO"
                     /locus_tag="VBD85_001836"
     CDS             2635..3969
                     /gene="trmFO"
                     /locus_tag="VBD85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO7134362.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDINKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            4047..>4310
                     /locus_tag="VBD85_001837"
     CDS             4047..>4310
                     /locus_tag="VBD85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5613 domain-containing protein"
                     /protein_id="HEO7134363.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIIISERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQ"
BASE COUNT         1425 a          754 c          830 g         1301 t
ORIGIN      
        1 caagaaataa gagtaaaaaa acttagctac ttaaagtagc taagttttta ttcccactca
       61 acagttgcag gtggtttact tgtaatatca taaacaatac ggttaacgtg atcaacttcg
      121 ttaacaatac gtgtcgaaat tttcttaagt acatcccaag gaagttgcgc aaagtcagct
      181 gtcatgccat caattgaagt aattgcacgg atagcaattg tatagtcata agtacgacca
      241 tctcccataa caccgacaga acgtacgcca gtattaactg tgaaatactg ccatacgtca
      301 cgatctagac cagctttagc gatttcttca cgaagaatag catctgattc gcggactgtt
      361 tcaagttttt cttcggtaat ttcacccata acgcgaatag caagaccagg cccagggaat
      421 ggttgacgcc atacaacttc atcaggcatt ccaagtgcag taccaagagc gcgaacctca
      481 tctttgaata atgtgttaag tggctcaatc aattcaaatt gcatatcttc tggcaatcca
      541 ccaacattat ggtgtgactt gattgtttgg gctgtttctg tcccagattc aataatatct
      601 gtgtagaggg taccttgagc aaggaaatca acgcctttaa gtttacttgc ctcatcatca
      661 aaaacgtaca cgaactcatt tccgatgatt tttcgttttc tctcgggatc ttcaacacca
      721 gaaagaagat ctaggaagcg ttttgaagca tcaacacgga taatattaag tccaaattta
      781 ccaccaagca tatccataac ttggtcacct tcatttttac gtaataaacc atgatctaca
      841 aaaatacatg ttaattgatc accgatagca cgttgtagga gtactccgac aacagatgaa
      901 tcaacaccgc cagatagtcc aagaagtact ttacgatcgc ctacagtttc acgtattttt
      961 gcaatttcca tatcaatgaa gttatccatt gaccaatcac cacgtgcgcc acagatatta
     1021 acagcaaaat ttttaaggat atcattgcca tatactgagt gacgaacttc ggggtggaat
     1081 tggataccat aaaattgttt ttcagtattt tccattgcag caaatggaca gtcaacagag
     1141 tcaccaacga gatggaaacc ctcaggaatt tcagtgacag catcaccatg gctcatcaaa
     1201 acaagttgtt cttgaggagt gccagcaaag agtgctgatt ctgagcgtag gcgaagtgct
     1261 gattgaccat actcacgatg accagcttct cccgcaggaa gaaccttacc accgagttta
     1321 tgggtaatca gttgcatgcc ataacaaatt cctaagattg gaattcctaa ttcaaaaatt
     1381 tcttcatcga ttccaaaagc gccatcagca tatacggaat ttggaccacc tgaaagtacg
     1441 ataccaatag gattaatatc gcgaatttca tcagccgtaa ttttatgtga ttttaactca
     1501 gaaaatacac caaattcacg gatacgacgt gcgataagct ggttatattg actaccgtaa
     1561 tctagaacga taattttttg gatatcattc aatatagaaa tatcagtcat tgtttccctt
     1621 tctttgtagc taattttaaa aacagctgta cctataccat tttagcatat tttatataaa
     1681 ttaacatcat taaaataatt gattaaacgc tttcttatca agaaatattg cttatgattt
     1741 ttctgaatta tttcacgatt ttagagttag aaatatatag aagtatggta aaatgaagtg
     1801 tagattctag gaggagagaa tattgttacc agcatatatt aaaattcacg acgctattaa
     1861 aaaggaaatc gataaaggga cttggaagat tgggcagcgg ttacctagcg aaagagattt
     1921 agcggatgat tatagtgtaa gtcgtatgac acttcgccaa tcaattacat tgttagttga
     1981 agaaggtatt ttggagagac gagttggtag tggcacctat gttgctagtc atcgtgttca
     2041 agaaaaaatg cggggaacaa ctagttttac agaaattgtc aattctcaag ggcgtaagcc
     2101 atccagtaaa ctaatctcat ttcaaagaaa actagcaaat gaaaccgaaa ttcaaaaatt
     2161 aaatttatca cagtcagatt atgtggttcg tatggaaagg gtacgttatg ctgataaggt
     2221 accgttggtt tatgaggtag cctctatacc agagaattta attaaaggtt ttgaacaatc
     2281 agaggttaca gaacatttct ttaaaacctt aacagagaat ggctacgaaa ttgggaaaag
     2341 ccaacaaact atatatgcta gaaatgctag tgaacgtgtt gcttcgcatt tagaagttaa
     2401 cgcaggccat gctattttgg cattgacaca agtttcatac tttactgatg gtaagccttt
     2461 tgaatatgtt catgggcaat atgttggaga tcgttttgag ttttatttag agaataacta
     2521 gtagtttaat gaataacgtt ttttgaagaa gtagctatgt atgttataat ggaaaaataa
     2581 attatataga aagatttgag gaagtagttg actactttca agaggtatta gttcatgtct
     2641 caatcttaca ttaatgttat cggcgctggt ttggctggtt ctgaagcagc ttatcaaatt
     2701 gctaaacgtg gaataccagt taagctttat gaaatgcgtg gggttaaatc aacccctcaa
     2761 cataaaacag ataattttgc agaattagta tgttccaatt cattccgcgg tgattctcta
     2821 acaaatgcag ttggtctttt aaaagaagaa atgcgtcgtt tggattctat cattatgaga
     2881 aatggggagg cacatcgagt tccagcaggt ggagccatgg ccgttgatag agaaggttat
     2941 tctgaagctg ttactgagga aatccataaa catccattaa tcgaagtaat tcgtgatgaa
     3001 atcacagata tccctggaga tgctattaca gttatcgcta caggaccatt aacaagtgat
     3061 agtctggcag ctaaaatcca tgaacttaat ggtggtgatg gtttttattt ttacgatgcc
     3121 gccgctccaa ttgtcgataa aaatacgatt gacataaata aagtttacct taagtctagg
     3181 tatgacaaag gtgaagcagc ttatctaaat tgtccaatga ccaaagaaga atttatggct
     3241 tttcatgaag ctttaactac agctgaggaa gcaccactaa attcttttga gaaagaaaaa
     3301 tacttcgaag gttgtatgcc tatcgaagtg atggctaagc gtggtattaa aacgatgctt
     3361 tatggtccaa tgaaaccagt tggtttggag tatcctgagg actacaaagg accgcgagat
     3421 ggtgaattta aaacaccata tgctgttgtt caattacgtc aagataatgc tgcaggctct
     3481 ctttataaca tagttggttt ccaaactcat cttaagtggg gagaacaaaa acgtgtattt
     3541 caaatgattc ctggtcttga aaatgctgaa tttgtccggt atggtgttat gcatcgcaat
     3601 tcttatatgg attcaccaaa tcttttaaac caaacctttg ctacacgtaa gaacccaaat
     3661 cttttctttg ctggacaaat gaccggtgtt gaaggttatg tagaatcggc agcaagtggt
     3721 cttgtagcgg gaatcaatgc tgtccgtcgc ttcaatgggg agagtgaagt agttttccca
     3781 caaactactg ctattggtgc actgccacat tatattacac atacagacag caaacatttc
     3841 caaccaatga atgttaactt tggtatcatc aaagaattag aaggtccacg aattcgtgat
     3901 aagaaagagc gttatgaagc tattgctaca cgagctttga aagatttaga aaaatttctc
     3961 aactattgat atattcattt gactttgcca acaagcaagg tcaaattttc tatattgatt
     4021 tttaaaaatg tatgaatgga gtaaatatgc actataaaga attacttgag tcatccgaaa
     4081 aagcttattt ggaaactaat cggtataaac attttaagaa tataatcatt tcagagcgtt
     4141 atttttcaaa ctatattagc tacaaaataa tgcctacagt aagtgagttt ttacaggata
     4201 tgatctattt agcagatgaa caaaaagatt atcagtctga ttttgctttt gtattttttg
     4261 aggaaaatgc tgaattgagc agtgaattac agagaacttt atcacaacaa
//
LOCUS       DATRSD010000114         4246 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRSD010000114 DATRSD010000000
VERSION     DATRSD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.115"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            37..1068
                     /locus_tag="VBD85_001838"
     CDS             37..1068
                     /locus_tag="VBD85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7134364.1"
                     /translation="MKLSKQLYKSLPLLTVVLCVGALQQNVEAKAKHYKTTSHVETQY
                     VSTSSKKILPFTHNKQIKVGSLDNLGRATYSHIQLRDADEPKIKRERLTYNPTGWHNY
                     KFTTEKGKTTWLMDRGHLVGYQFSGMNNVPENLVTMTKYLNTGFSENNPDGMLYYENR
                     LDSWLANHKNFWLDYKVTPIYEGNNLVPSRVELQYVGIDKQGKLLEIKLGGGKEQVDE
                     YGVTTVTLENTSPLAKIDYKTGMLIKEDGKQAEEGEDPNSDADENEAAIESASDIEEN
                     TNTNTSESDTNNVAPQNRIVYVANKGRSNTYWYSLENIKNANTANIVQMTEQEALNQH
                     KHHSTTEAQ"
     gene            complement(1279..2091)
                     /locus_tag="VBD85_001839"
     CDS             complement(1279..2091)
                     /locus_tag="VBD85_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7134365.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILTF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2081..2791)
                     /locus_tag="VBD85_001840"
     CDS             complement(2081..2791)
                     /locus_tag="VBD85_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7134366.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(2794..3237)
                     /locus_tag="VBD85_001841"
     CDS             complement(2794..3237)
                     /locus_tag="VBD85_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO7134367.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3323..4174)
                     /locus_tag="VBD85_001842"
     CDS             complement(3323..4174)
                     /locus_tag="VBD85_001842"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO7134368.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPVCAGLGGG
                     STDAAATIRALNRLWNLQMDYDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
BASE COUNT         1557 a          837 c          626 g         1226 t
ORIGIN      
        1 ggccttcaca aatatcattt agaagggttt aggtttatga aattatctaa acaattatac
       61 aaatcactcc cactattaac tgtcgtgcta tgcgttgggg cattacaaca gaatgttgaa
      121 gctaaggcta aacattataa gacgacatcc catgttgaga cacagtatgt aagtacttct
      181 tcaaaaaaaa tcttaccgtt tactcataat aagcaaatca aggtaggttc attagataac
      241 ttaggaagag caacatattc tcacatacaa ttaagggatg cagatgagcc taagataaaa
      301 agagaaagac tgacgtataa tccaaccggt tggcacaact acaaatttac tactgaaaag
      361 ggtaaaacaa cttggcttat ggaccgaggt cacctcgttg gttatcaatt ttcaggaatg
      421 aacaatgttc ctgagaacct agttactatg accaaatatc taaatacagg atttagcgaa
      481 aataatcctg atggtatgtt atattacgaa aatagattag atagttggtt ggcaaatcat
      541 aaaaatttct ggcttgatta caaagtgact ccaatatacg aaggaaacaa tttagtacct
      601 agccgagttg aacttcagta tgttggaatt gataaacaag gaaaacttct agaaatcaaa
      661 ttaggcggcg gcaaagaaca agttgatgaa tacggcgtca caactgttac attagaaaat
      721 acgtctcctc tcgcaaaaat tgattacaaa actggtatgc taatcaaaga agatgggaaa
      781 caggcagaag aaggtgaaga tccaaatagc gatgctgatg aaaacgaagc agctattgaa
      841 agtgcatctg atatagaaga aaatactaac actaatactt ctgaatcaga tacaaataat
      901 gtagctcccc aaaatagaat cgtctacgtt gcaaataagg gtcgttcaaa tacttattgg
      961 tatagtttag aaaacataaa aaatgcaaat accgctaata tcgttcaaat gactgaacaa
     1021 gaagcgttaa atcaacacaa acatcacagc actactgaag cacaataatt acaatttact
     1081 gccggcaaaa ttgccggtag tattttttat cctaaaacgc aaaaatagat tcacattgcc
     1141 ccttctatca acttaagcca gaaaacttag tagatacctc atcgagataa tttctcaacc
     1201 gatccctcat aaaatatatt cgaaaaaaac agggagataa ataaaaaatc tatcctagaa
     1261 taagatagat ttttttattt aacgtttgcg aagcaagcct actaagctaa ctaataggaa
     1321 aatgccaata aaaatcatcg ttattgttgc actagcaggt gtctcccaat aatacgaaag
     1381 gaaaatacca gctaccattc ccacaaatcc aattaacata cccagaaaaa taacagtttt
     1441 aaaatttcga cctaaccgca tagcaatact agctggtaaa accataatag ttgacaccaa
     1501 tagagcacct gcggcaggaa ttgttaatgc aatagcaata cctgtaacaa cattgaacaa
     1561 aatagacatt gtacgcacag gcaatccatc tacaaaagct gtatcctcat caaacgttaa
     1621 tatatacatt ggtctaataa ataggatggt taaaataaaa gtgattaatg caataacaaa
     1681 caaagcaatc acttgttcct tcccaatagt aataatagaa ccaaataaat actgttcaag
     1741 actgacatta ccaacattat gagctttact cataacgatt aaagatatag ccaaccccat
     1801 cgacatcagg atagcagttg atatttccat ataatgtttg tatacagtac gcaaatactc
     1861 taatacaact gccgccaaag taacaacgaa aatagttgac catgtaggcg agatacctaa
     1921 aacaacgcct aatgcaacac cggctaagga aacgtgactc aaagtatcac tcatcaaact
     1981 ctgacgacgt aaaattagga agatgcctaa aattggagca aaaatactaa tagccacaac
     2041 tgctaagagc gctcgttgca taaaatcata ggaaagcata tcaagtagca tctgattcaa
     2101 cctccatttc gtttgtatgg acattgaaac aacgccatgg taaagattgg ttcctaacaa
     2161 gatgaatatt tcggtcagca taccccttta cttcatcagg atcgtgagta atcatcaaaa
     2221 cagacttacc atgcttatga gcattatgat gcattaactc ataaaatttc tcagtagtcc
     2281 ctgcgtccat accagttgtt ggctcatcca atacaaaaat atctggatca gaagcaaaca
     2341 tcctagcaat gacagccctc tgcttttgac cgccagataa acttcctatc tttttatggc
     2401 gattatccca cataccaaca gcttctaaac taaccctaat atgttcttca tcatgtttag
     2461 tcagtcttct aaaccaacca tttctagggt atcgtccaga tttcacaaat tcataaactg
     2521 acgatggaaa gccagcgtta aaacttgcaa tctgttgagg taaataggca atacgtaatt
     2581 tttttccttc tttgttttct ttagagatat taactgtacc cactttgggc gttaaaatac
     2641 ctaaagtagc ctttattaaa gttgatttag cagccccatt ttcacctgtt aaggtaacaa
     2701 actccccact gtctaaatgg taattaaccc cttccagcac aggatcgcta tcgtactgaa
     2761 aagtaagacc actaactgta atatatcgca tgattaccct tctaattctc tagagaaaag
     2821 atcaagaaaa cgttctaaaa cgaccttttc gtcctttgaa aaatgattta ctaatcttcc
     2881 gtaaacccct aacgtattgt catgatgatg tgtgtgttca tctgcaatgg gtttagcaag
     2941 ttcagataac tcaaaataag taatacgagc atctttagaa tctttattcg ctttcaacat
     3001 atcctgagaa attaaacttt ttactgcttt agttacagct gcctgactaa tatttaactt
     3061 cttagctaaa tcagaatttg tcaactgctc ttgtgataaa agcatcagaa tgtgttcttg
     3121 cgtattagtc aatttaacat cactttgaca agtaccaaac aataattcat gttgattttc
     3181 tgctttaagc aagatttgac tcactaaatg gtctaatttt tgttctaaaa ctgtcatata
     3241 tacctctttt ttgttaacca gtaaattata tcacgaagat atagaagaat caatcataga
     3301 taggtgaaga agataaaacc ttttatctca acaacctaac tttataaact tctttgcaaa
     3361 aacctttcat actattaaaa acacgatcag cttttttctc tgtagaacac attgaaaaaa
     3421 cagttggtcc acttcctgtc attaatgcaa catcggctcc agaatttaac atacgttctt
     3481 ttattgtact tataactgga tttttagtaa ttgtaatatc ctcgagtgaa tttcccatag
     3541 atttgaccat taactgataa tctgatgaca aaatagcaga ctttaataaa tcaatatcaa
     3601 ctctacttat agacttacaa tcaatatctc taaaaatgga tttagttgaa ataccaaaat
     3661 ccggcttaac cagaactatc caacatggtc tcaatgtcgg taagggttta acaatttcac
     3721 ctttacctaa tactaacgaa catcccccac caagacaata aggaacatca ctaccaattt
     3781 taaaaccaat agcaaccatt tcgtcatagt ccatttgaag attccataat cgattaagag
     3841 ctcttattgt agcagcagca tcagtagaac caccccccag tcctgcacag acaggaatgg
     3901 atttttctaa tctaatatga acacctttat taataccata ttgatttttg attatatctg
     3961 cagctttaaa cacatcatta tcattattta aaggcatttt gctactatca gaatcgataa
     4021 caatacaatc ttcctttagc tcagaaatgg taacgtagtc attaagatca atactaacca
     4081 taatcatagc taattcatga taaccatcgt cacatcgtcc tttaatatct aatcctaaat
     4141 taagtttggc aggagctttc tcaaaaattt tcataaaacc tccctaataa aatatagaat
     4201 atccatatta taacataaca aatgacaaat tcggacaatt aagatg
//
LOCUS       DATRSD010000115         4201 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRSD010000115 DATRSD010000000
VERSION     DATRSD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.116"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            62..1876
                     /gene="glmS"
                     /locus_tag="VBD85_001843"
     CDS             62..1876
                     /gene="glmS"
                     /locus_tag="VBD85_001843"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO7134369.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2039..2368
                     /locus_tag="VBD85_001844"
     CDS             2039..2368
                     /locus_tag="VBD85_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO7134370.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFVKK"
     gene            2503..3144
                     /locus_tag="VBD85_001845"
     CDS             2503..3144
                     /locus_tag="VBD85_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7134371.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            3154..3783
                     /locus_tag="VBD85_001846"
     CDS             3154..3783
                     /locus_tag="VBD85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7134372.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            3797..>4201
                     /locus_tag="VBD85_001847"
     CDS             3797..>4201
                     /locus_tag="VBD85_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7134373.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVT"
BASE COUNT         1381 a          623 c          840 g         1357 t
ORIGIN      
        1 caattcaaaa agaaagttta gatagaaagt ataagagaaa gctcttattg gtaataataa
       61 tatgtgtggt atcgtaggtg tagttggaaa tacaaatgca acggatattt taattcaagg
      121 cttagaaaag ctagagtacc gaggatatga ctcagcaggt atttttgtag taggtgataa
      181 caaaagtcaa cttgtgaagt cagtcggtcg aatcgcggag cttcaggcaa aagtaggaga
      241 ttctgttagt ggtacgacag gtatcggtca tacacgttgg gcaacccatg gtaaacctac
      301 tgaaggaaat gcacatcctc acacttcagg atctggtcgt tttgtattgg tacacaatgg
      361 tgttattgaa aattaccttc aaattaaaga aacttactta acaaaacata acctcaaggg
      421 tgaaactgat acagaaatag ctattcattt agttgaacac tttgttgaag aagataactt
      481 atcagtgctt gaagccttta aaaaagcctt acatattata gaaggatcat acgcatttgc
      541 tcttattgat tctcaagatg ctgatacgat ttacgtagct aaaaataaat cacctcttct
      601 aataggtttg ggaaatggtt ataatatggt gtgttcagat gccatggcta tgatacgtga
      661 gacaagtgaa tatatggaaa tccacgataa agaactcgtt attgttaaaa aagatagcgt
      721 tgaagttcaa gattatgatg gtaatgtcat tgaacgtggt agctacacag cagagcttga
      781 cttatcagat attggtaagg ggacttatcc attctacatg cttaaagaaa ttgatgagca
      841 accaactgtg atgcgtaagt tgatttcaac ttatgcgaac gaatctggag atatgaatgt
      901 ggattctgat attataaaat cagtccaaga agcagatcgt ctttacatat tggctgcagg
      961 aacgtcatat catgctggtt ttgctgctaa gaccatgatc gaaaaattaa ctgatacacc
     1021 tgttgaactt ggagtttctt cagaatgggg ttacaacatg ccacttttga gtaagaagcc
     1081 aatgtttatt ttacttagtc aatcgggaga aacagcagat agtagacaag ttttagttaa
     1141 agcaaacgaa atgggaatcc ctagtttaac tataacaaat gtgcctggct caacactttc
     1201 tcgtgaagca acctatacga tgctcattca tgcaggacct gaaatcgctg ttgcttctac
     1261 aaaagcatat actgctcaag ttgcaacgtt agctttctta gcaaaagcgg taggagaagc
     1321 aaatggtaag gcagaagcaa aagattttga tttggttcat gaattgtcaa ttgtagcaca
     1381 atcaattgaa gcgaccttat cagaaaaaga tgttatctct gagaaggtag agcagttgtt
     1441 aatctcgaca cgtaatgcct tctacattgg tcgtggtaac gactattatg ttacaatgga
     1501 ggctgcactg aagctaaaag agatttctta tattcagaca gaaggttttg ctgcaggtga
     1561 attaaaacat ggtacaattt cgttgattga agacaatact cctgttattg ctttaatatc
     1621 tgctgattca actattgcag cgcatactcg tggaaatatc caagaagttg tttctcgcgg
     1681 agcaaatgct ttgattattg tagaagaagg gttggaacgt gaaggtgatg atattattgt
     1741 taataaagtt cacccattct tatcagccat ttcaatggtt attccgactc aattaattgc
     1801 ttactatgct tcattacaac gtggcttaga tgttgataaa ccacgtaatt tggcaaaagc
     1861 tgtaactgtt gaatagactc gttacactaa aatacgctaa tattaactac aaaatattaa
     1921 aaattgtaaa aaagcctatt tttgaaggaa aataggcttt ttcctattgt caagaagcct
     1981 ataatcagtt agaatgttac ttgctgacta ttttattaat taatttaagg agagaaaaat
     2041 gtcattacca aattgcccta agtgtaactc tgaatatgtc tatgaagacg gtattttact
     2101 ggtttgtcca gaatgtgctt acgagtggaa tccagaagaa atcgaagaag aagtaggttt
     2161 aattgtattg gatagcaatg gaacgcgctt atcagatgga gatactgtga cagttattaa
     2221 ggatttaaaa gtaaaaggag cgcctaagga tattaagcaa ggcactcgtg ttaaaaatat
     2281 tcgtttagtt gatggcgatc ataatatcga ttgtaaaatt gatggttttg gagctatgaa
     2341 actaaaatct gagtttgtaa aaaaatagct ttcaggcaat tggcaacggt caattgcttt
     2401 ttgaatatga cttgatttca tgcattttta ttaaaaaagc tctatgacat tggtttgaat
     2461 tttgatataa tgagaacatc tattttttat ttagggggga ccatgtctta tattttagaa
     2521 atcttaccta gcttattagt aggtgcgcaa gtgacattgc aagttttctt tattgttatt
     2581 tgtttatcaa ttccacttgg tattgttgta gccttcctaa tgaaagttca ttttaagccg
     2641 cttcaatggc tactgacaac ttatatttgg attatgcgag gcacgcctct gttattacag
     2701 cttatctttt tttattactt tttaccaagt gtagggattt cttttgatcg tatgccagct
     2761 gcgattattg cttttacgat gaattatgca gcttattttg cagagatttt tcgaggaggt
     2821 attgagtcta ttcctaaagg gcaatatgaa gctgcaaagg tattaaagtt ttctaagttt
     2881 caaacggttt ggtacatagt attacctcaa gtcttcaaaa tagtcttacc gagtgttttc
     2941 aatgaaacga ttactttagt gaaagattcc tcattggttt acatacttgg tgtgggcgac
     3001 ttacttctag aaagtaagac agccgcaaat agggatgcaa cacttgcacc aatgtttata
     3061 gcaggtggta tttacctatt attaattggt cttttaacta ttttatctaa acaagttgaa
     3121 aagcgtttta actactataa ataaggaggg acgatgttag aattaaaaaa tatttctaaa
     3181 tgctatgggc aaaaagaaat ctttaaagat ttcaatctga ctgtagaaga aggtaaaatt
     3241 ctctctttag taggaccttc tggaggtgga aaaacaacac tattacgtat gttagctgga
     3301 ttggaaaaga ttgattcagg gactattgtt cacgatggta aagaagtatc tgttgatcat
     3361 ttggaaacac taaatctttt aggattcgtc ttccaagact ttcaattatt cccacattta
     3421 acagtattag ataatttgat cctctctcct gttaaaacga tgggattatc aaaagaactt
     3481 gctaaagaaa aagccttagt attacttgaa cgcttgggac ttaaagacca tgctttagtc
     3541 tatccatttt cattatctgg ggggcaaaag caacgtgtcg cactagctcg tgcgatgatg
     3601 attgatccac agattattgg ttatgatgag ccaactagcg ctcttgatcc agagttgcgt
     3661 caagaagtag aaaaactaat tttacaaaat agagaaacag gtatgacaca aattgtagta
     3721 acacatgatc ttcaatttgc tgaaagtata tctgatacga ttctcaaaat taatcctaag
     3781 tagacttggg ggtagattgt tgactcacaa aaatatatta ttaaccatta tatttggatt
     3841 atttatgatt atattatcag catgtggtat gtctaataag gaaatggctg gtattgataa
     3901 ttgggaacat tatcaaaagg aaaagaaaat tactattgga tttgataata cttttgttcc
     3961 tatgggattt gaaagtcgtt ctggtgacta taccggcttt gatattgatt tagctaatgc
     4021 tgtttttaaa gaatacggta tttcagtgaa atggcagcct attaactggg atatgaaaga
     4081 aactgaactt aataatggta atatagacct tatttggaat ggttattcaa aaacggcaga
     4141 acgtgctaaa aaagtcgctt ttacaaaccc atatatgaat aatcatcaag taattgttac
     4201 t
//
LOCUS       DATRSD010000116         4113 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRSD010000116 DATRSD010000000
VERSION     DATRSD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.117"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..2809
                     /locus_tag="VBD85_001848"
     CDS             <1..2809
                     /locus_tag="VBD85_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEO7134374.1"
                     /translation="TSKIISRLKPIKRQRYSFVENIINKLEMKDVSKEKQEDIAIQLT
                     VVWTNRILFLKLLESQLVLFNKDESYRFLTYEKLPNFEEIYGLFFAVLAKKVSERNDR
                     VQEKFGYVPYLNSSLFEETEIEISRDGIGIDRLPEGDIEIFSKTALKGVDKKRKKGNI
                     NFIEYLFEFLDSYDFSTSISHHEKSKNDLINASVLGLIFEKINGYRDGSFYTPGNITM
                     YMSRKAIRTAAVDKVNELLGWNCETVEEIKFAIGHSVENARKVSQAIDDLKVCDPAVG
                     SGHFLVSILNEIIALKSDLNILFDADGNYIGNLIQCYVINDELVIQDMSGNNFLYQTG
                     NQQSEQIQKAIFNQKRHILENSLFAVDINPSSVNICRLRLWIELLKSSYYYQDTDTNQ
                     PVLTTLPNIDINIKVGDSLLHKFEFDYEFDMRKTDFKDYLSLVKDYKETNNKRVKAEI
                     WEKIERLKHSFDDTASSPELKKLSRLISERNKAGAISLFEDENKNSKEVAELNKQLAK
                     AEKELEYRLKNPLFAKGMEWRMEFPEILGEAGEFIGFDLVIGNPPYIFARNQSFTDEM
                     KSYYSRTYQVSEYQANTYTIFMELAYQLLRKGGTFSYIIPNNFLTIQTNSRVRQFITE
                     QTSDVVLINSLDKIFADASVDNCIIFFKKNAPNWIEVAELYHGEFNTVGRVAPDFFGE
                     IPTFSISMVKYRQAIDIFWKVNHFPILGRPEISTVKSGIKAYETGKGNPKMTAQDKNN
                     RIYHARERSDDSYRPYLDGENVSRYKLTWNGEYVKYGENLAAPRDKEIFEKPRILVRQ
                     IPSKSTYAVEAVYTDSDVINDLNSMVITDIQVNPFYLLGILNSRLISLWFFMKFDKFQ
                     RRLFPQFKVNELGDFPIPDATDSQQEDIAQVVEQLMEEMKKDSPDTEKVNQLNLEIDE
                     LVMDLFDLAEEEKQTVRDFEV"
     gene            2823..3314
                     /locus_tag="VBD85_001849"
     CDS             2823..3314
                     /locus_tag="VBD85_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134375.1"
                     /translation="MAKSLQSQFRDIERNLVRETNKMVKRANKKIKPVILPTQLDSSK
                     TALNFNEYHQDNSTHVSVNGDLINSQIGGQNNALNITYTEIEIERVFENIREYSRTLD
                     EEEQTELVTILNEVQEVGKVDNHQTFFEKHPILAMALSASVSWGVENGIDRLVSVISN
                     TFK"
     gene            3366..>4113
                     /locus_tag="VBD85_001850"
     CDS             3366..>4113
                     /locus_tag="VBD85_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134376.1"
                     /translation="MLDLEKILRGMISAKFKDLRGSIPPDIISNGQRTAIYNIEKGKN
                     PKSGNFVTDTLLEDYSSYFGMDKSDLIFGDALLLELTLYHVFSQIFYQIVPDDNRIGL
                     KIDRNKIQNNIDSSIIEPFLELFYIFGDFGRWYNIRRKDDDESDKDIDYTTMYEIVWR
                     LIKSRVVSSFNQHVVGEMFEKDKVFNFNQINNVFNNWYHKHFVKIIIPEALKKLESDS
                     IFKMGFMVRNLIENFLVLDLPLSYLEDVPLE"
BASE COUNT         1423 a          611 c          805 g         1274 t
ORIGIN      
        1 gacttcaaaa attatttcac gcttaaaacc catcaaacgt cagcgatatt cttttgttga
       61 gaatatcatt aataaattgg agatgaaaga tgtttcaaaa gaaaagcagg aagatattgc
      121 tattcaacta acagttgtct ggacaaatag aattctattt ttgaaactgt tagaatctca
      181 gcttgtactc tttaacaaag atgaatctta taggttcttg acgtatgaaa aactccctaa
      241 ttttgaagaa atttacggtc tattctttgc tgtacttgct aagaaagtat cggaaagaaa
      301 tgatcgagtg caagaaaagt tcggttatgt cccttatctg aactcatcac tgtttgaaga
      361 aacagaaata gaaatttcta gagatgggat tggtattgat agattaccag agggagatat
      421 tgaaattttc tccaaaacag cccttaaagg cgtagataaa aaacgtaaaa aagggaacat
      481 caattttatt gaatatctct ttgagttttt ggattcttat gacttctcga cctccatcag
      541 ccaccatgaa aaatctaaga atgaccttat caatgcgtca gttctgggat taatttttga
      601 gaaaatcaac ggctaccgtg atggctcttt ttatacgcca ggtaacatta caatgtatat
      661 gtcacgtaaa gcgattcgta cagcagcagt ggacaaagtt aatgaattgc ttggttggaa
      721 ttgtgaaact gttgaagaga ttaagtttgc gattggacat agtgttgaaa atgctcgtaa
      781 agtcagccaa gcaattgatg atcttaaggt ttgtgaccca gcggttggat ccggtcactt
      841 ccttgtttca atcctgaatg aaattattgc tcttaaaagt gatttaaata ttttgtttga
      901 cgctgatggc aactatattg gaaacctgat tcaatgttat gttatcaatg atgagctagt
      961 cattcaagat atgtctggaa ataatttttt gtatcagaca ggtaatcagc agtcagaaca
     1021 aattcaaaag gcaattttca accagaaaag gcatattctg gaaaattcgc tctttgcagt
     1081 ggatattaac ccaagctcgg tcaatatttg ccgtttgcgt ctttggattg aactcttaaa
     1141 atcttcttat tactaccagg atactgatac caaccaacct gttttgacga ctttgccaaa
     1201 tattgatatc aatattaaag taggcgatag tctcttacat aagtttgaat ttgactatga
     1261 atttgatatg agaaaaacag atttcaagga ctatctatct ctagttaaag actataagga
     1321 gacgaataat aaacgagtta aagcagaaat ctgggagaaa atcgaaaggc tcaaacattc
     1381 ctttgatgac acagcttcaa gccctgaatt aaagaaatta tctcggctca tatctgaacg
     1441 aaataaggct ggtgccatta gcttatttga ggatgaaaat aaaaattcta aagaagtagc
     1501 ggaattaaat aagcaactag caaaagctga aaaggaattg gaatacagac tgaaaaatcc
     1561 attattcgct aaaggtatgg aatggcgaat ggaatttcct gagatcttgg gagaagctgg
     1621 cgaatttatc ggctttgacc ttgtaattgg gaatccgcct tatatctttg ctcgtaatca
     1681 gtcctttaca gatgagatga aatcctacta ttcaaggact tatcaagtaa gtgaatacca
     1741 agccaatacc tatactatat tcatggaatt ggcttatcaa ctcttacgta aaggtgggac
     1801 attctcttat atcattccaa ataattttct gaccattcag accaattcaa gagttcgtca
     1861 gtttatcacg gagcagacga gtgatgttgt tctgattaat tctctggata agatttttgc
     1921 ggatgctagc gtggataact gtatcatttt cttcaagaag aatgcgccaa actggattga
     1981 agtagcagaa ttgtatcacg gcgagttcaa cactgttgga cgagttgcgc cagatttctt
     2041 tggtgaaatc cctacctttt caatctcgat ggtcaagtat cgtcaggcga ttgatatctt
     2101 ctggaaagta aaccattttc caatactagg acgaccagaa atttcgactg taaaatctgg
     2161 aatcaaagcc tatgaaactg gaaaagggaa tcctaaaatg accgctcaag ataagaataa
     2221 tcgaatttat catgcaagag aaaggtcaga tgattcatac cgtccctatc ttgatggcga
     2281 gaatgtatca cgctataaac tgacttggaa tggtgaatat gtcaagtatg gtgaaaattt
     2341 agctgcacca agagataaag aaatttttga aaaaccacga attttagttc gtcagattcc
     2401 aagtaaatca acttatgctg ttgaagctgt ttatactgat agtgatgtta ttaacgactt
     2461 aaactccatg gtcatcacag atattcaggt taatcctttt tatcttttgg gtattttgaa
     2521 ctctcgctta atctctcttt ggtttttcat gaagtttgat aaattccaac gtcgcctatt
     2581 cccacagttc aaagttaatg aactaggaga ttttccaatt ccagatgcta ctgattccca
     2641 gcaagaagac atagcacaag ttgttgagca actgatggag gagatgaaaa aagattctcc
     2701 tgacactgaa aaagtcaatc agcttaatct ggaaattgat gaacttgtca tggacttgtt
     2761 tgacttggct gaagaagaaa aacaaacagt tagagatttt gaagtataga aagtaggtgt
     2821 aaatggcaaa aagtttgcaa agtcaattca gagatattga gagaaattta gtcagagaaa
     2881 cgaataaaat ggtgaagaga gctaataaga agataaaacc tgttatcctt ccaacacaac
     2941 tagattcctc taaaacagca ctaaacttta acgaatatca tcaagataat agtactcatg
     3001 tttctgttaa tggagatttg ataaactcac agattggtgg acaaaataat gcgttaaata
     3061 ttacctatac tgagatagaa attgagagag tatttgagaa tatcagagag tactctagaa
     3121 cattagatga agaagaacag acagaattag tgacaatttt aaatgaggta caagaagtcg
     3181 gaaaagttga taaccatcaa actttttttg aaaaacaccc tatacttgca atggctctta
     3241 gtgctagtgt aagctgggga gtggagaatg gaattgatag attagttagt gtcatttcaa
     3301 atacatttaa gtgaaaagtt ttttatttga tggaaataga aaatggagag tttgaggtgt
     3361 agatgatgtt ggacttagag aaaattttga ggggaatgat ttctgcaaaa ttcaaggact
     3421 tgaggggaag cattccacca gatatcattt caaatggcca aagaactgca atctacaaca
     3481 ttgaaaaagg taaaaatcct aaaagcggaa attttgtaac cgatacttta ctggaggatt
     3541 actctagcta ttttggaatg gataaatcag atcttatctt tggagatgct ctattattag
     3601 aactgactct ttaccatgta ttttctcaaa ttttttatca aatagtacca gatgataatc
     3661 gtattggctt gaagattgat aggaacaaaa ttcaaaacaa tattgattca agcattattg
     3721 agccattttt agaactattc tatatttttg gggattttgg gcgatggtat aatattagaa
     3781 ggaaggatga tgatgaatca gataaagata tagattatac gacaatgtat gaaatcgttt
     3841 ggcgtttgat aaaaagtaga gtggttagtt catttaatca acatgtagtt ggagaaatgt
     3901 ttgaaaaaga caaggtattt aattttaatc aaatcaacaa tgtctttaat aattggtatc
     3961 ataagcattt tgtaaaaatc ataatccctg aggcgttaaa aaaattggag tctgacagta
     4021 tttttaagat gggatttatg gttaggaatt tgattgaaaa ttttttggtt ttagatttac
     4081 cactatcata tttagaagat gttccattag agg
//
LOCUS       DATRSD010000117         4088 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRSD010000117 DATRSD010000000
VERSION     DATRSD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.118"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..184)
                     /locus_tag="VBD85_001851"
     CDS             complement(<1..184)
                     /locus_tag="VBD85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HEO7134377.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPY"
     gene            complement(245..1150)
                     /locus_tag="VBD85_001852"
     CDS             complement(245..1150)
                     /locus_tag="VBD85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7134378.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            1408..2622
                     /locus_tag="VBD85_001853"
     CDS             1408..2622
                     /locus_tag="VBD85_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7134379.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVSIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKAILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
     gene            2630..4012
                     /locus_tag="VBD85_001854"
     CDS             2630..4012
                     /locus_tag="VBD85_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7134380.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
BASE COUNT         1268 a          601 c          819 g         1400 t
ORIGIN      
        1 agtaaggtaa aagattttgt tttaacagac tagatgatat taatggaatt aaaaaaacaa
       61 aaataggaaa agcaacatct ggaaacataa tgttaaattt tttaagttta aatattttga
      121 caagaacctg tgaaaatata gggattaaaa caagaaataa agaagcaata attttgtata
      181 tcataggtct attttatacc gattttggta agaagtaaac tgattttcaa tagaattagc
      241 gaaactactt ggttaaaaaa gctttataaa gtaggagagc aattccagat aattcaacat
      301 cttttttata aatcataatg tactctgaaa ataacttatt cttatgaaaa tggtagacat
      361 tgcactcttt atccaaatag cgatgaatga gagtttgtgg aacaaatgtt gctcccatcc
      421 ctgacaaaga taggctagct gctgtaggaa cggtactagt tgtaatcatc tgatttaatg
      481 acatgtgatg aagctcatag aattgtgcaa ttgttttgcc aattccgtac tctaatggtg
      541 ttaggataag aggttcattg tctagaactg aaataggata ttcaaaagga ataatctgtc
      601 cacgtttttc agcgttgtag agttggctgg tggtaggtat cattaaaaac aattcgtcgc
      661 ggtaaatagt ggttgatgca attccatctt tataaaggat aggagccatc ccaacagcta
      721 ggtcaatatc accactcgct accaattgtt cagaaatatt ttgattgttt tcgagtaatt
      781 tgacagaaat ttctgggtgc tccaagttaa atttgggaag tacatttgcc agaatagaac
      841 ttgctaagcc agagttaatc ccaagtgtga tttctccttt tttatcagaa cggatgagat
      901 atagttcttt ggccatttga cgctcaatca tatcaatttc ttttaggtag aatagatatc
      961 tctgtccagc ataagttaaa aaggtttggt gtccttggct ctggatgagt tttatttcta
     1021 actcattttc taattgcttg atcactttgc ttaggtaagg ttgagaaata aaaaggtgtg
     1081 cagcggcttt agtgatgttt gagtatagct cgatactctc tagatacatt aatttttgtt
     1141 gatttttcaa gactttataa ccagtttgat atccttagat taataataag ttatgatacg
     1201 ttatggctat aagtttacta tcattttgag ctaaaatcaa tctatttaat ttagtagtat
     1261 aacatgatat attcataact atttagttat agaaatatct aaaaaaattt atttgtaaaa
     1321 aattattttc aaaagtagac tatataagag cagattgtta gaataatagc cttattttaa
     1381 ctgttcatat tttaaggagg aaagcaaatg gccaatgcat ctttgcgaca tcaattattt
     1441 gaaaaacttg accaaaaatg tgatcaaatg gtttctattc gtcgctattt acatgaaaat
     1501 cccgaattat catttaaaga gacaaaaaca gcagcttata tttcagattt ttataaaggt
     1561 aaggactgtc atgttcagac acaatttggt ggtatgaatg gcgtcgtagt tgatatctat
     1621 ggtgataaag cgacagataa gcccatcaaa cacattgctt tgagagccga ctttgatgct
     1681 cttccaattc aggaagagac agggctttct tttgcttcaa aaacagcggg cgtcatgcat
     1741 gcttgtggac atgatgccca tacagcttat ttattgattt tggctgagag cttgattgaa
     1801 ttaaaatcag aattttcagg tcatatccgt atcttacatc aaccggctga agaggtgcca
     1861 ccaggcggag caaaagcaat gattgaagca ggatgtttag atggcattga tgctgtcctt
     1921 ggtatccatg ttatgtcaac gatggaagag ggaacggtac aatatcacgc aggtcccatt
     1981 caaacagggc gtgccacttt taaagctatt ttgcaaggta agggtggaca tggttccatg
     2041 ccacatcgtg caaatgatac aattgttgca gcttctagtt ttgttatggc agcgcaaacg
     2101 attgtcagtc gtcgagttaa tccatttgac acagcagtag ttactatcgg atcttttgat
     2161 ggtaaaggtt ctgctaatgt gatcaaagat agtgtaacct tagaaggtga tgtgcgcgtg
     2221 atgtctgaag agacacgagg tgttgttgag gaggaattta agcgtattct agatggaata
     2281 gcacaaactt atggtgttag ttatcagtta gactatcaga atgattatcc tgttttagtt
     2341 aataacagtg aagtgacaca gaaagttgca aatagtttaa aatctgttgc tataaaagaa
     2401 attttagatg tcattgattg cgaccctcaa acgccgtctg aagattttgc ctattatgct
     2461 caaacaatcc cagcttgctt cttctatgtt ggagcacatg aagaggggca accatattac
     2521 ccacatcatc atccaaaatt ccaaattgca gaatcatcat tgatggtatc tgctaaatca
     2581 atggcaacag cagcgcttgc tatgcttgta gaaggagaat agataacata tgttaacgac
     2641 aaaggaaaat ttcaaaggaa ataacaaatt attggtggga attgtactcg ctgtactatc
     2701 attttggtta tttgcacagt ctattttaaa catgggacca gatgttcagt catcattagg
     2761 tatttcatct ggtgcaatgg atattggtgt ctcaagtacg gcattgtttt caggtttatt
     2821 tattgttgta actggtggtt tagcagataa acttggtcga gttaaattta cttttattgg
     2881 tctttgctta aatattattg gttctctact aattgtttta gctaatggag cagtgctatt
     2941 tattatggga cgtatttttc aaggattggc agcagctttt attatgccat caacaatggc
     3001 cttggttaaa acgtactatg atggtaaaga tagacaacgt gcagttagtt tctggtcaat
     3061 cggatcgtgg ggtggctcag gtctatgttc ttattttggt ggagcggtgg caagtactct
     3121 aggttggcgt tatgtcttta tcttttcaat catcgcatca gtagttagtt ttctattaat
     3181 tttaggaacc ccagaaagta aaaatgttgg acaaaaaact cactttgatt accttggttt
     3241 aattattttt attatttcaa tgttatcgtt aaatattggg atatctatgg ctcaggaaca
     3301 tggattgatg aacgttattc cattgagtct ttttacagtg atgcttattg gatttgttct
     3361 gttttattat gttgaaacac gtaaatctaa tagttttatt gactttcatc tgtttgaaaa
     3421 tcgattttac ttaggtgcga caatatcaaa tttcttactg aatgcagttg caggaactct
     3481 gattgttatt aatacttaca tgcaacaagg tcgccagcta acaccaaagg ttgcaggtga
     3541 aatgtcttta ggctatttgg tttgtgtact aattgctatc cgagttggtg agaaaatttt
     3601 acaacgtttt ggtgcccgta agccaatgct tttgggagct atgagtacat ttgttgggat
     3661 atttttaatg actttggtca atattcaagg acctttgtat cttgtgttag tctttgttgg
     3721 ttatgcttta tttggaacag gcttggggat ctatgcgaca ccatcaacag atacagctat
     3781 ttctagtatt cctaatgaaa aagtaggttc tgcatctgga atttacaaaa tggcaagttc
     3841 actaggtggt gcaattggtg tagcaacttc aattgcgatt tatcatgctt tttctggtaa
     3901 tgcagatttt cacaaagcag ctttgtgtgg attaatttta aacctagttt tttgtagtct
     3961 atcgatctta tcaattcttt ttgttatccc taagaaaatt gaggataaat aaataacaat
     4021 acttgagatt caaaatctca agtattgttg taataatgta aaaggaattg ggaaagaaat
     4081 ttaaaata
//
LOCUS       DATRSD010000118         3902 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRSD010000118 DATRSD010000000
VERSION     DATRSD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.119"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..370)
                     /locus_tag="VBD85_001855"
     CDS             complement(<1..370)
                     /locus_tag="VBD85_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134381.1"
                     /translation="MSIFSDKLTKYRKEQGFTQQEVADKIGINRGSYSNWENGKREPT
                     LENVVKLAKLFKTTTDELLGQTIYSKVDLLRFLDDFDVSDIKNFEKEDVDKLKFAIMF
                     EMTRNKVNALNLKDDLILKYK"
     gene            620..958
                     /locus_tag="VBD85_001856"
     CDS             620..958
                     /locus_tag="VBD85_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134382.1"
                     /translation="MSRYSTQVFYEFTDEEISKFIEVNNIVNKTNNLDQAIKQVWGDL
                     DTQLEQDSKEMIADLRKDFLSYQKKSLLLIQSLGKQNHSLSQRLTKLSERLDRLEEEQ
                     DKGFLSKWKK"
     gene            973..1332
                     /locus_tag="VBD85_001857"
     CDS             973..1332
                     /locus_tag="VBD85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134383.1"
                     /translation="MSLKNTDIAVKNWEEKDYQLVEVQEWFEYKGNEREHAGWKYVIV
                     LPRLRYEKVAVKVANPNGEVPFISSEKLAELGSATVTFTNLNIGMYTRTAKNSEFVSL
                     ELSAKADGISLAKEPAK"
     gene            1683..3029
                     /locus_tag="VBD85_001858"
     CDS             1683..3029
                     /locus_tag="VBD85_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HEO7134384.1"
                     /translation="MSRKRTTTYIFEQQLHAEYWDWEEDKKALFTNWESNKTKIFQEI
                     HRRIKTLEEDIPAKVAFIVHDKDIKFGIKSVEPHIHAYIEFASRRDLSVLASTLGLLP
                     QYIEPSGQGKYGKVNSKAYLIHAKSPDKHQYAPSEVETFGTFDYEAFIEDNRADFSKR
                     YAVAKREKSDESLDKVFQEIINGNLTENDIFADEELTFLWSYNQTRLDEAFRAYGKIA
                     SKRTLRELENGEFKPTIIYVHGSSGIGKTTLALEVIEEIIKRAKEEGLNWKMYSAGAK
                     NIFDEYFGEEVILLDDPRSDSLLPADWLKLLDPLNKSYLSARYKNKLVIGRLIVITNY
                     QSLKSFFGKIQNEDLNQYIRRFNNVLEISKKKGNHDKEKDRFYNLSEVKELNYREYYQ
                     TGYDQEIQLHFGEEGIYCTDNKADFINRVLDEHILPRILPQQKKRPQTQAREKGNA"
     gene            3098..3292
                     /locus_tag="VBD85_001859"
     CDS             3098..3292
                     /locus_tag="VBD85_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO7134385.1"
                     /translation="MIATVTKDDLVALGFSEGTSRSIIRKGKHLLVQRGFYVYDNKRI
                     GTIPATIAEELLDLKLTSKV"
     gene            3297..>3902
                     /locus_tag="VBD85_001860"
     CDS             3297..>3902
                     /locus_tag="VBD85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HEO7134386.1"
                     /translation="MAIRKAKNGTWTVDVSGGIDPVTFKRIRIVRKGLKSKKDAIELE
                     YHLRVVELKEKNRDTFVTSDMLFSMLEKEDNQNHRKISYLTTQRNNYDRHIKTYFQQV
                     DMKKLTYEHIYRFRESLKEKSTKQNSEEKLSNNTINKIMILVKKMFDSGIRNELITKN
                     PCQNLRKLPIKKPELNFWGIEEFLKFKSLITEDEYQYNLFFT"
BASE COUNT         1434 a          597 c          710 g         1161 t
ORIGIN      
        1 gtttatattt taaaataagg tcatctttta agttaagtgc atttactttg tttcttgtca
       61 tctcaaacat aattgcaaat tttaatttat ctacatcttc tttttcaaaa tttttgatgt
      121 cgcttacatc aaaatcgtct aaaaatctca gtaaatctac tttcgaatat attgtttgtc
      181 ctaataactc atctgtagta gttttaaata gcttggcaag cttaacgaca ttttctagag
      241 ttggttctct tttcccattt tcccaatttg agtatgatcc tctattaata ccaatcttat
      301 cagcaacctc ttgttgagta aatccttgct cttttctata tttggttaat ttatctgaga
      361 atatcgacat tttttctcct ttatgcttca aaatgacact ttttcttgac aaccgcgtcg
      421 cattgaagct ataatatatt tgtgtcaata agacgcacta tgattatacc acatcaccta
      481 aagatagtaa aaggaggaga aacatattga ttacaaatta atcgcaagtc aaacagaaaa
      541 atcagtatct acgattaaga agtggcgttt acgaatagaa aagatttctg gatatagctt
      601 taaaaaagct aaacataaag tgagtcgcta ttcaacgcaa gtcttctatg aatttactga
      661 tgaggagata tctaagttta ttgaagtcaa taacatagtt aataaaacta acaacttaga
      721 tcaagctatc aaacaggttt ggggtgacct tgatacccag ctcgaacaag attcaaaaga
      781 aatgattgct gacttaagaa aggattttct atcttatcaa aagaaaagct tgttattaat
      841 ccaatctctt gggaaacaga atcattcctt atcgcaacgt ctaaccaaac ttagtgaacg
      901 gttagatcga ctagaagagg aacaagataa aggattcctc tcaaaatgga aaaaatagaa
      961 agaagaaaaa ttatgagttt aaaaaatact gatattgcag taaagaattg ggaagaaaaa
     1021 gactatcaat tagtcgaagt gcaagaatgg tttgaataca aaggaaatga acgtgaacat
     1081 gcaggttgga aatatgttat tgtcttacct cgcttacgtt atgaaaaagt cgctgtaaag
     1141 gtggctaatc caaacggtga agttcctttt atttcaagtg agaaattagc tgagttaggt
     1201 tctgccactg tgactttcac taatcttaat attggtatgt atacccgtac ggctaagaat
     1261 tcagaatttg tatcactcga attaagtgca aaggcagatg ggatttcatt agctaaggaa
     1321 cccgctaagt aatctaagat ttttcatgtg agtgcggaga gttcactcac tacgagcgag
     1381 acgacgaagt catagctcgt aagggagtga gaaacgtgtc cgcatgcaca acaatgaaaa
     1441 atcttagatt gattttgtaa agagttttct gggatttaat ttgcccaatg cccaattgtg
     1501 acgggcgcac cattcagggc ttgtcgacag aatggtgtgt aacccaataa taccaatgct
     1561 ttcagcgatt ttcaaaaaaa caagaatcct tgaaaatctt gggcaaattg atgggtaaat
     1621 taatgggcaa attaaactaa tttgcccaag aatgataaaa attttctata gaaaggagtc
     1681 taatgagccg aaaaagaaca acaacttata tctttgagca acaattgcat gcggaatatt
     1741 gggattggga agaagataaa aaagcattat ttaccaattg ggaaagtaat aaaacaaaaa
     1801 tcttccaaga aatacatcgt cgcattaaaa ccctcgaaga agatattcca gcaaaggtcg
     1861 cttttattgt tcatgataaa gatatcaagt ttggcatcaa gtctgtagag cctcatatcc
     1921 atgcttatat tgaatttgcc agtcgtagag acttatctgt attagctagt actcttggtc
     1981 tcttaccgca atatattgag cctagtggtc aagggaaata tggaaaggtc aattccaaag
     2041 cctatttaat tcacgcaaag agtcctgata aacatcagta tgctcctagc gaagtggaaa
     2101 cttttggaac atttgattat gaagctttta ttgaggataa cagagcagat ttttcgaaac
     2161 gctatgcggt tgccaaaaga gaaaaatcag acgagagttt ggataaagtc tttcaagaaa
     2221 ttatcaatgg taatctaaca gaaaatgaca tctttgcgga cgaagagtta acgtttcttt
     2281 ggtcttataa tcaaacaaga cttgatgaag cttttagagc ttatggcaag atagcttcta
     2341 aacgaacctt aagggagtta gagaatggag agttcaagcc cactattatt tacgtgcatg
     2401 gatcttcagg aattggtaag acgactctag cacttgaagt gatcgaagaa attataaaga
     2461 gagctaaaga agagggtctc aactggaaaa tgtacagtgc tggtgctaag aatatttttg
     2521 atgagtactt tggtgaagag gttattcttt tagatgatcc aagatctgat agtttattac
     2581 cagcagactg gttaaaactc ttagatcctc tcaataagtc ttacctatca gcacgctata
     2641 agaataaatt ggttattggt cgtctaattg tgattaccaa ttatcagtct cttaagtctt
     2701 tctttggaaa gattcaaaat gaagatttaa accagtatat cagacggttt aataatgttc
     2761 tagagatttc taagaaaaaa ggaaatcatg acaaagaaaa agatagattc tacaatctat
     2821 cagaagttaa agaattgaac tatagagaat attaccagac cggatatgat caagagattc
     2881 aattacattt tggggaagaa ggtatttatt gcactgacaa taaagcagat tttattaatc
     2941 gtgtattaga tgaacatatc cttcctcgca tattaccaca acaaaaaaag cgtccccaaa
     3001 ctcaggcaag agaaaaagga aacgcttagg acaagccgag gagcaggaac tcctcaacct
     3061 gtccttaatt ataccatagt tattaaggag aatacacatg attgcaaccg taacaaaaga
     3121 tgatttagta gccttaggat tttcagaggg aacctcccgt tccattattc gtaaaggaaa
     3181 acacctcctc gttcaacgtg ggttctatgt ttatgacaat aaacgtatcg gaaccatacc
     3241 tgctacaatt gcggaggaat tattagattt aaaattaact tctaaggtat aaagccatgg
     3301 caatacgtaa agcaaagaat gggacttgga ctgttgatgt ttctggcgga atagatcctg
     3361 taacttttaa aagaattcgt atagttagaa aaggactaaa aagcaaaaaa gatgctattg
     3421 agttggagta tcacttaaga gttgttgaat taaaagaaaa gaacagagac acatttgtaa
     3481 ctagtgatat gttattttca atgcttgaaa aagaggacaa tcagaatcac agaaaaatta
     3541 gttacttaac gactcaaagg aacaactatg accgacatat aaagacatat tttcaacaag
     3601 tagatatgaa aaaattaaca tatgaacata tctacagatt tagagagtca ctgaaggaaa
     3661 aaagtactaa acaaaatagt gaagaaaaac taagtaataa tacaatcaat aaaataatga
     3721 tattagtaaa aaaaatgttt gattctggta taagaaatga attaatcact aaaaatcctt
     3781 gtcaaaattt aagaaaatta cctataaaaa aaccagaatt aaatttttgg ggaattgaag
     3841 aatttttaaa attcaaaagt ttaattactg aggatgaata tcaatataat ttatttttca
     3901 ct
//
LOCUS       DATRSD010000119         3845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRSD010000119 DATRSD010000000
VERSION     DATRSD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.120"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            275..571
                     /locus_tag="VBD85_001861"
     CDS             275..571
                     /locus_tag="VBD85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO7134387.1"
                     /translation="MDLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(594..1133)
                     /locus_tag="VBD85_001862"
     CDS             complement(594..1133)
                     /locus_tag="VBD85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO7134388.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPEVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            1272..3590
                     /locus_tag="VBD85_001863"
     CDS             1272..3590
                     /locus_tag="VBD85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO7134389.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQDGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLEMLSDGVTAYTTGVHKLSEGSQKLDDKSQALMEGSEK
                     LTDGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            3657..>3845
                     /locus_tag="VBD85_001864"
     CDS             3657..>3845
                     /locus_tag="VBD85_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEO7134390.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQDVYNGLIG"
BASE COUNT         1338 a          576 c          706 g         1225 t
ORIGIN      
        1 tctcaaaaat gcaatacaac aaaatgcttt aattattaca ttacatactt taaaagccta
       61 tttgataggc ttttttgtta tatacttttt agtgagtcat tatatttaat gaaccaaaaa
      121 gattttaaac taacactaga ataaaaatca acactgtcag tttgtaatga gtaatttttt
      181 actttgtaaa aaggatatag tagaattaat gttacaaata cttgagaatg ataacttatc
      241 tcgtcgaatc ttatataact aaggagattt tattatggat ctaaatgata gattaaaaat
      301 agaagaaatg gaagagaaat atgatagctt caaacctaga atcaatgcat tagtagaagc
      361 tatagatgat tttcaaaagc actatgaaga ttacgtgaaa ttaagagaat tttatggtag
      421 cgaagactgg tttagactat cagaacaaac agaaaataat ctcaaatgcg gtgtactgag
      481 tgaggatcag ctctttgatt ttataggaga acataacgag cttgtaggcc aatttttaga
      541 catgtcttct caaatgtatc gtcatttata atgtcagtcc ttataggctg gttttaagtt
      601 tgaggtaata aactgagtag cacttgtgta atttgttgag ggctttcttt ttttccccgt
      661 gttatccaca tttgatagac tccaaaaata gcatgactga gatagacact ataatacaaa
      721 cggttgatat cagactttgt tgcatctgga ttgacctcag gtaattcttt agaaagcatt
      781 aattgtaatt tattaaggat gtaggtctga atttcttttg ttccattttg ggttaagaga
      841 gcagcaaata atgattctct atctaagaat tgaaaaattt cgagcaaggc atcttctttt
      901 ttaaattgat tgcgatcaaa aatatattcg actttattga agagtgactg ttggtattga
      961 tcaatcattt catatttatc tttataatga gtataaaaac tagaacgact gattccagct
     1021 gttttagtta gattaatcgt tgaaatttca tcgaagctct gatcttttaa taacgtaatc
     1081 atagctgctt caatagctct ttttgttttt tctctcctcg tatcgtttac catattaatt
     1141 accccttaat atttttgtac atttttaaac acagtgtcca aaaacaagat ttttatcttg
     1201 aaattatcta tcatcactgt ataatatatt ttatcaaaaa aatgaacaat atgtccaaaa
     1261 taggagaaaa aatgttagac gaattaaaag caatcataaa aagcccaaaa ttatggatta
     1321 cgatggctgg ggttgcactg attccaacgc tatataatgt tatctttcta agctcaatgt
     1381 gggatcctta tgggaatact aaaaatttac ctgtggcagt cgttaaccaa gataaatcgg
     1441 ctaaattaaa tggaaaaact atttcaattg gcaaagatat ggaggacaat ttgtctaaaa
     1501 atgatagctt agatttccat tttacaacgg ctaaaagagc agaaaaagaa ttagaaaagg
     1561 gtcactatta tatggttatt acctttccta aagacctttc tagaaaagcg acaactctca
     1621 tgacagaaaa accagaaagg ttaaatatta cttataaaac tactaagggg cgtagttttg
     1681 tagcttcaaa aatgagtgag acagcagcta ataaactaaa agatgaggta gcagaatcta
     1741 ttacagggac ttatactgaa tcagttttta aaaatatggg ttctatgaaa acaggtatca
     1801 ataaagctgc tgatgggagt caagaattat taaatggttc taataagcta caggatggca
     1861 gtcaaacctt gacaagtaat ttagatgttt tagcaagctc tagtcaaact ttttctggag
     1921 gagctaataa actaaatagt ggaataaacc tttatacaga tggtgtgggg accctatcta
     1981 acgggttaga aatgttatca gatggtgtta ctgcttacac aacaggtgtg cataagttga
     2041 gtgaaggtag tcaaaaatta gatgataagt ctcaagcatt gatggaagga tcagaaaagt
     2101 tgacggatgg tttacaacaa ttgtcacaag caactcaact aaaaccagag caagaacgta
     2161 ctttgcagaa cctttcagat ggattaaaga atctaaatca gattattaca aatttacaat
     2221 cgacagcaac tactgatagc gatactaata gtaagttatt taatttttta tcaactattg
     2281 aatcaagtac aaaggctttg atgaatacgg ctgctgctga taaacaaaag caaatgactg
     2341 cggtacaatc aacgtcagct tttaaaagtt taacccctga acaacaatct caaattacct
     2401 cagcggttac tggaacacct acttcagctg agacaattgc agcaaatatt tcttcaaata
     2461 tagaaaatat gaaaactgtg cttagtgaag catctagttc agccccttct aataataact
     2521 cacaaaattt tcaaactttg tcaggaacag ctaacaattt agtattaaaa gctatctcag
     2581 atttagataa aattcaaaaa ttgccaacgg ctactaagca gttatatcaa ggtagtcaaa
     2641 ccttgacaaa aggaatcacg gattatacga atgctgtagg gcaattacgc aaaggtgcag
     2701 aaactttaga tagtaaatcc aatcaattga tttcaggaac acaaaaagca agtcagggag
     2761 ctcaaacttt agatagtaaa tcggatcaat tgagagatgg agcggggcaa ttagctagtg
     2821 gttctgatag aattgcagat ggttctaata agcttgctgg gggaggacat cagttgacag
     2881 atgggttgac agagttgtca ggtggtgttt ctcaattatc aagttctttg ggtaaagctg
     2941 gggaccagtt gtccatggtt tctgtaaaca aggataatgc caatgctgta tctagcccag
     3001 tcactatcaa gcatgaagat tatgatagcg ttgatacgaa tggtgttgga atggcaccat
     3061 acatgatttc agttgcgttg atggtggtag ctttatcagc caatgttatt tttgcgaaag
     3121 ctttatctgg caaagagcca gctaatcgtt ttagttgggc taaaaataaa ttattaatca
     3181 atggattcat tgcaactcta gcagcaacta tcttattttt tgcagttcaa ttcataggtc
     3241 ttaaaccaga ttaccctgga aaaacctact ttattatcct attgacagca tggactttga
     3301 tggcattagt aactgcttta gtgggatggg ataataggta tggttccttc ttgtcgttat
     3361 taatattatt attccagctt ggttcaagcg caggaactta cccaatagaa ttgagtccta
     3421 aattctttca aacaattcaa ccatttttac cgatgactta ctctgtttca ggattaagag
     3481 agaccatctc gttgacggga gacgttaacc atcaatggag aatgctagta atctttttag
     3541 tatcatcgat gatacttgct cttcttattt atcgtaaaca agaagattaa tagaaagtat
     3601 ctagtgatag actaacagta tgatatggta tgtcaaagga tttaggagga gaagatatgt
     3661 ctactttaac aataattatt gcaacattaa ctgctttgga acatttttat attatgtatt
     3721 tggagacgtt agccacccag tcaaatatga ctgggaagat ttttagtatg tctaaagaag
     3781 agttgtcata tttacccgtt attaaacttt ttaagaatca agatgtatac aacggcttga
     3841 ttggt
//
LOCUS       DATRSD010000120         3684 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRSD010000120 DATRSD010000000
VERSION     DATRSD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.121"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..246
                     /locus_tag="VBD85_001865"
     CDS             <1..246
                     /locus_tag="VBD85_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HEO7134391.1"
                     /translation="TQKLSAFGQKKQTLTISHNYLENTYERLYLPKTVKVVKSDTLAF
                     DTNGGNSSLAKIQFECYRKTVTYQLYIGSGNYRKKEN"
     gene            218..517
                     /locus_tag="VBD85_001866"
     CDS             218..517
                     /locus_tag="VBD85_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HEO7134392.1"
                     /translation="MVIIVRKKIRAYILLESLVGLAILVTITRLVISEINRQQSHIAR
                     RQHEQEVLILARMAVQTDQKILEANGVNIRIEESKNKIVLFEDNKEILHVSKVKG"
     gene            471..932
                     /locus_tag="VBD85_001867"
     CDS             471..932
                     /locus_tag="VBD85_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO7134393.1"
                     /translation="MKIIRKFCTYQKLRVKAFTLLECLVALVTITGALLVYQGLTKLL
                     AQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDF
                     RKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            910..1281
                     /locus_tag="VBD85_001868"
     CDS             910..1281
                     /locus_tag="VBD85_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO7134394.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKTDTLQVPLKSNDNKASHKH"
     gene            1396..2370
                     /locus_tag="VBD85_001869"
     CDS             1396..2370
                     /locus_tag="VBD85_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7134395.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDMVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDGIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIK"
     gene            2402..3595
                     /locus_tag="VBD85_001870"
     CDS             2402..3595
                     /locus_tag="VBD85_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7134396.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
BASE COUNT         1306 a          550 c          710 g         1118 t
ORIGIN      
        1 actcagaaat tgagtgcatt tggtcagaag aaacaaaccc ttacaatctc tcataattat
       61 ctcgaaaata cttatgagag actttattta cctaaaactg taaaagtagt caaaagtgat
      121 acacttgcat ttgacactaa tggagggaat tcaagcttgg caaaaattca atttgaatgt
      181 tatagaaaaa ctgtcacgta tcaattatat ataggaagtg gtaattatcg taagaaagaa
      241 aattagagcc tatattttac tggaaagcct agtgggatta gctattttag ttactattac
      301 aagattagta ataagtgaaa taaatcgtca acaatcccat attgcaagaa ggcaacatga
      361 gcaagaagta ttgatattag ctagaatggc tgtgcaaact gatcaaaaaa tattagaagc
      421 aaatggtgtt aatattcgta ttgaagaaag taaaaataaa attgtgctct ttgaagataa
      481 taaggaaatt ttgcacgtat caaaagttaa gggttaaagc atttacgctt ttagaatgtt
      541 tggtagcatt ggttacaatc acaggagctt tactagttta tcaaggactg acaaaattgt
      601 tggctcaaca gatagtagtg atgtcttctt ccagtcagtc tgaatgggtg ttattaactc
      661 agcaactaaa tgcagaattt gaaggcgctc atctggaata tttaagacag aacaaacttt
      721 atttacgtaa gcaagataag attgtaacct ttggcaaatc taataaagat gatttccgta
      781 agacaggtta tgatggtcga ggttatcaac caatggttta tgggttagac aattgtcaaa
      841 tgagtcagac caaaagtatg gtaaaacttg ttttttattt taaggacggg ttaaaaagga
      901 cattttacta tgattttaaa gaagaaactt aaagctggca tccttttgca agctattgta
      961 ttggctgctg tatttacact cgtcttacag ttttatctag caagaatatt ggcaacagaa
     1021 agacagtatc atagccaaat tgaagcaagt aaagcttatt taacagcgca gttagcttat
     1081 aaaacaatag aaggtgactc catatcagga aaatgttatt ttacaggagg atatgcttca
     1141 tacctccaag aagggaacta tttacaagtt aaggttacat tggataaggg aggaaactat
     1201 aaccataagt tttatcgtaa aacagacact ctccaagtac ctctcaagtc taatgataat
     1261 aaagcaagcc ataaacatta aaaatataca gtgatgaaat attagtaaga caaggttttg
     1321 cttaaaaaca ggatttttgt ttgcttctct tttcagatgc tgtgtttttt gttatgataa
     1381 attctggagg aaatcatgaa ttttgaaaaa attgagacag cctatgagct gattttagaa
     1441 aatatccaaa cgattgagaa ccaattaaaa actcatattt atgatgcctt aattgaacag
     1501 aactcttatt accttggttc aagttgtgat ttagatatgg ttgtggtgaa taaccaaaaa
     1561 ttacgtcaac ttgacttaag tcaagaagaa tggcgtcgca ctttccagtt catttttatc
     1621 aaatctgcgc aaacagagca attacaagct aatcatcagt ttacgccaga tagtattggt
     1681 tttatcttgt tatttctttt ggaagaatta acgagtcaag agacagtgga tgtcttggaa
     1741 attggaagtg gaactgggaa tttagctcag actctcctca ataacagctc gaaagagtta
     1801 aattatatgg gcattgaagt tgatgatctt ttgattgatc tatcagcaag cattgctgaa
     1861 attataggtt ctagtgccca atttatccaa gaggatgctg ttagaccaca aattttgaaa
     1921 gaaagcgatg taatcattag tgatttacca gttggctatt atcctaatga tggtattgct
     1981 aaacgatatg ctgtatcaag ttctaaagag cacacctatg ctcaccatct attgatggag
     2041 caatctctta aatatttgaa aaaagatgga atcgctatat ttttagcacc cgaaaacctt
     2101 ttaacaagtc cacaaagtga tttgctgaag gagtggttaa aaggatatgc agatgtcatt
     2161 gccgttttaa ctctaccaga aactattttt ggaagtcgtc aaaatgcgaa atctatattt
     2221 gttctcaaga agcaagcaga acaaaaacca gaaacctttg tatatccgct gacagatttg
     2281 caaaatcgtg agaatatggc aaacttcatt gaaaattttc aaaaatggag cagagaaaat
     2341 agtcattact caaaaaatat gataaaatag agattgtgaa aacgctttag agaggtaaat
     2401 tatgtcaaaa acaatagcaa ttaatgctgg aagttcaagt ttgaaatggc aattatatga
     2461 aatgccagag gagaaagtag ttgccaaagg gattatcgaa cgtattggtt tgaaagattc
     2521 tatttcgact gtaaaatttg acgataaaaa agatgaacaa attttagata tcgtagatca
     2581 tacccaagct gttaaaattc ttttagaaga tctaacaaaa catggtatta tcaaagactt
     2641 taatgaaatt acaggtgttg gtcatcgtgt cgttgctggt ggtgaatatt ttaaagagtc
     2701 tgctttagta gatgataaag ttgtagaaca ggttgaagaa ttatcagctt tagctccact
     2761 ccacaaccca gcagctgcag cagggattcg tgcgtttaga gaaatcttac cagatatcac
     2821 gagtgtatgt gtatttgata cagccttcca tacaacaatg caacctcata cctacttata
     2881 tccaatccca caaaagtact atactgatta taaagtacgt aaatacggtg cacacgggac
     2941 aagccaccaa tacgttgcac aagaagcagc taagcaatta ggacgtcctt tagaagaatt
     3001 aaaattaatc acagcacatg tgggtaatgg tgtttctatt acagcaaatt atcatggaca
     3061 gtctattgat acatcaatgg gattcacacc acttgcagga ccaatgatgg gaacacgttc
     3121 aggagacatt gatcctgcga ttatccctta tcttgtagct aatgatccag aattggaaga
     3181 tgcagcagct gttgttaata tgcttaacaa acaatcagga ttgcttggag tatcaggaac
     3241 ttcaagtgat atgcgagata ttgaagcagg tcttcaaagt aaagatccaa atgcagtgtt
     3301 agcctataat gtctttattg accgtattaa gaaatttatt ggacaatact tggcagtatt
     3361 aaatggcgcg gatgctatta tttttactgc aggtatggga gaaaatgcgc cattaatgcg
     3421 tcaagatgtt atcgcaggat tatcatggtt tggcattgaa cttgatccag aaaaaaatgt
     3481 ttttggttat tttggagata tcacaaaacc agattcaaaa gtgaaggttt tagttattcc
     3541 tactgatgaa gaattaatga ttgcgcgtga cgttgagcgc cttaaagcta aataagttat
     3601 caaaaaattg ataagaccag aaaagttgat agtttaacta tcaacttttt tgtcttttga
     3661 acagatatgt aaaatgtact tgac
//
LOCUS       DATRSD010000121         3653 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRSD010000121 DATRSD010000000
VERSION     DATRSD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.122"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..189
                     /locus_tag="VBD85_001871"
     CDS             <1..189
                     /locus_tag="VBD85_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO7134397.1"
                     /translation="FYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFY
                     NTYNEHYCQKVLGNNKEC"
     gene            338..1159
                     /gene="mutM"
                     /locus_tag="VBD85_001872"
     CDS             338..1159
                     /gene="mutM"
                     /locus_tag="VBD85_001872"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO7134398.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1150..1743
                     /locus_tag="VBD85_001873"
     CDS             1150..1743
                     /locus_tag="VBD85_001873"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO7134399.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            1867..2568
                     /locus_tag="VBD85_001874"
     CDS             1867..2568
                     /locus_tag="VBD85_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134400.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            2558..3653
                     /locus_tag="VBD85_001875"
                     /pseudo
     CDS             2558..3653
                     /locus_tag="VBD85_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1234 a          527 c          687 g         1205 t
ORIGIN      
        1 ttctatgaaa aaactgtttt cttatatgta actggatact atcatttaaa acttggtgat
       61 acttcttcag gaaaagaaga tatgaggaaa gctttacaga tttttaaata tttaggtgaa
      121 gattcatttt acaatactta taatgaacat tattgtcaaa aagtacttgg caataataaa
      181 gaatgctaat gagtgcaagt ttatgagtaa tttttaaagc ttttgtctca aaatgataat
      241 aagaaaggaa aatagaaatc gaatggtcag gtgacatcaa gaacgtttgg aaattattgc
      301 tctaagcaat gactattgat ttttagacta tatatttatg ccagaattac cagaagtaga
      361 aaccgttaga aaaggactag aaagacttgt agttaatcaa gaaatagctt ctataacgat
      421 taaagtccct aaaatggtga aaactgattt gaatgacttt atgataagtc taccaggaaa
      481 aacaattcaa caggtattga gacgagggaa gtatcttctt tttgattttg gtgaaatggt
      541 tatggtatca catttacgca tggaaggaaa atatctactc ttcccaaata aagtaccaga
      601 taacaaacat tttcaccttt atttcaaatt gactaatggc agcacattgg tttaccaaga
      661 tgtccgtaaa tttgggacat ttgagttggt tcgtaaatca tctctcaaag attattttac
      721 tcaaaaaaaa ttaggtcctg agccaactgc tgacacattt caatttgagc ctttttcaaa
      781 aggcttagca aactccaaaa agccaataaa acctttacta ttagatcaga gattagtagc
      841 aggacttgga aatatttacg tagatgaagt cttgtgggca gcaaaaattc acccacagcg
      901 tttggctaat caattgaccg aatctgaaac aagcttatta cacaaggaaa ttattcgcat
      961 tttaacttta ggcatcgaaa aaggtggctc cacgattaga acatataaga atgctcttgg
     1021 agaggacggt acgatgcaaa agtatttaca ggtctatggt aaaactgggc aaccgtgtcc
     1081 aaggtgtggt tgtttaatta agaagataaa agtaggaggg agagggacgc attattgccc
     1141 tcggtgtcaa tgcttatgac aaaaataata ggactgacag gagggatagc ttctggaaag
     1201 tcaacggtaa caaaaataat acgagaatca ggttttaaag tcatagatgc ggatcaagtg
     1261 gttcataaat tgcaagctaa gggtgggaaa ctttaccaag ctttattaga atggttgggt
     1321 cccgagatac ttgatgctga tggtgagttg gatagaccaa agctttctca aatgattttt
     1381 gctaatccag acaatatgaa gacatcagct aggctacaaa atagtatcat tcgtcaagag
     1441 ttagcatgtc agcgcgacca attaaaacaa acagaagaga tatttttcat ggatattcct
     1501 ttattgattg aagaaaagta tataaaatgg tttgatgaga tttggttggt atttgttgat
     1561 aaagaaaaac aattacaacg attaatggcc cgtaacaact acagtcgaga agaagcagaa
     1621 ttacgacttt cacaccaaat gcctttaaca gataaaaaaa gtttcgctag tcttattatt
     1681 gacaataatg gtgatttaat aactttaaaa gagcaaatat tggatgctct tcaacgttta
     1741 tagaactact aaatggataa tgtatttatc cattttttgt ctttattaca ctagttttaa
     1801 aaaacttgcg attaaaccac agtaatagta caatgatata aaaggtttac tttcaaagga
     1861 gtttttatgg agttagtaat tagagatatt cgtaagcggt ttcaggaaac agaggtcttg
     1921 agaggagcaa gttaccgatt ttattcaggt aaaataacag gggtcttagg taggaatggt
     1981 gctgggaaaa caactttatt taatatactt tatggggatc ttgcagctga caacgggacc
     2041 atttgtttat tgaaggataa tcacgagtat cctcttaccg ataaggatat tggtattgtt
     2101 tattccgaaa actaccttcc agaattttta acagggtatg aatttgtaaa attttacatg
     2161 gatttacatc cttcagatga tttaatgaca atagatgatt atttagattt tatggaaata
     2221 ggacaaacag agcgtcatag aattatcaaa ggatattctg atggaatgaa gagtaagctc
     2281 tcattaattt gcctgatgat ttctaagcca aaagtaattt tactagatga gccactgact
     2341 gcagttgatg ttgtatcaag tattgcaata aaacgccttt tgttggaatt aagtgaggat
     2401 catattatta tattatcaac tcatataatg gccttagcag aagatctatg tgatattgtg
     2461 gctgtattag acaaaggaaa actccaaaca ttagatattg atcgtaaaca tgaacaattc
     2521 gaagagcgtc ttcttcaagt gttgaaggga gatgaatatg acaagtaaag cattaatatg
     2581 gtctatatta cagattgata gtgctagagc ttataatagt tttttatatt accttcagaa
     2641 aatcccctgg ttgggaagaa aaatccctaa ccgatggttt aaaaccgatg atataaaagg
     2701 tgtagtacta tttattcgta tcattttctt tttcttaaga ggttttggtc gttccatagg
     2761 ttacgtacta tattgttatg ttttatcatt ttttgttaga agtggcatga agcagttaaa
     2821 tattttaccg caagaaggtt ttaccctaac tttaacattg atggtgatta cctctctttt
     2881 aattgtcata aatttctata taaaacttat agaacctaat gatatacaac aaactcatat
     2941 gattcgcctt ttcagaattg agccatcgag gtatttccaa tccgctgaac ttttagaagc
     3001 cagtagtgaa ctatttttta gaagtttgag ctttggcata tttttccatt taaatcactt
     3061 tgctttttgg catggtttga cttttgcttt atttttagca ggtagtcgcc ttggaatcaa
     3121 ggtgctctgt ttacctttat atggtagagg taaagaccaa gagaatccag agtctttact
     3181 aaatatttta ttttacttag ggattggtat aggagtcttc ctttctctct ttttccttct
     3241 tcttggcaaa cagttctctc catatccatt tttttcatgg tacaccggta ttgcaggact
     3301 tatcatatgg ttagttagtt actatcgatt aaaaacttat tctaaactta accacttgac
     3361 ctaccttacc ctaacgcatg agacgatgaa ggagaaaatt gcaaagattg ataatataga
     3421 gctgcttggg attgaaatga aagataggga tataaatgtc agtgaacttt ctcccctgct
     3481 atttgaaaat ttatcaggta tttcatattt aaacgctatc tttttagtat atacttgcaa
     3541 aggaaaattt tggtacgttt agctgtttta agtgttctgt ttggaataga actccttttc
     3601 ctgctatttt ttaaagaaaa gataaatatt ccgatgaatg agcatagttt ttc
//
LOCUS       DATRSD010000122         3641 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRSD010000122 DATRSD010000000
VERSION     DATRSD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.123"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..421)
                     /locus_tag="VBD85_001876"
     CDS             complement(<1..421)
                     /locus_tag="VBD85_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134401.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAILTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAK"
     gene            complement(540..1688)
                     /gene="nagA"
                     /locus_tag="VBD85_001877"
     CDS             complement(540..1688)
                     /gene="nagA"
                     /locus_tag="VBD85_001877"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO7134402.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKSVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(1966..2673)
                     /locus_tag="VBD85_001878"
     CDS             complement(1966..2673)
                     /locus_tag="VBD85_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO7134403.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQANNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2865..3176
                     /locus_tag="VBD85_001879"
     CDS             2865..3176
                     /locus_tag="VBD85_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO7134404.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            3197..>3641
                     /locus_tag="VBD85_001880"
     CDS             3197..>3641
                     /locus_tag="VBD85_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134405.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKI"
BASE COUNT         1200 a          721 c          536 g         1184 t
ORIGIN      
        1 atttagcatg acacaataaa gttacaccta aaagttcagg agcaatattt aagaaatagt
       61 taggatgctt aacaacccta aataaccagt gatctacaaa ttgatgattt ttatctacca
      121 ttaatttcac tgtccaaata cgaccaagta aacgtgttac tgtatgtaac ataaacacag
      181 aaaaaatcat caagctaagc ccgattacgc taacaaaatt aaaacttgct tttgttagaa
      241 tagcctcagt tacagatgaa aaataaatga taatatgaag aactgtgatt gcttttgaaa
      301 cacctacacc atattcttta gcaccatttt ttcttaaggc cttttcatta gctatagata
      361 gctttaaata agctaaacga ataataaaca tagcggccat taacccaata ataattgaca
      421 ttgtaaactc ctattgtttt gtattatatt acaacattat aagaaaaaat agttttattg
      481 tcaaactata tttcattaaa aattaataaa ttaaaaagaa ctagtaaaaa ctagttcttt
      541 caagattgcc aacgtttttc cccatcaagg tatgttgcaa ctaaatctag atgtttatct
      601 aatacaataa aatccgctgc ataaccttct tttatctgtc cacagacatt atcaatgcct
      661 acagacactg ccggaacata actcgccata cgcactgcct cttccggagt agcaattccc
      721 cattctacaa cattttttaa tccatctttg agcttaagaa tagaaccagc taaattacct
      781 ccatctttca aacgcgcagt tccattagac acaaccacgg gcaattcccc taacatataa
      841 tctccatcag gggcacctcc agctgccata caatctgtta ttaatgccac atgattatgg
      901 cctttttgtg tcattaaaat gtcacaagca acaggatcaa cgtgatgacc atcgcaaatg
      961 agttcggcat aggtattagg taaattataa accgcaccaa ccatacctgg ttctcggtgt
     1021 gtcaaaccac gcataccatt atacgcatgt acccaaactg aggcacccgc cttcactgcc
     1081 tttttagctt ctttatatgt cccattagaa tgacctaaag ctaccgtaac accttgcttt
     1141 gtgattgctg agacaaattc ctcaactccc tcacgttctg gtgccaaagc aatttttgta
     1201 atcaagcctt ttgcagcctt ctgccattga gcaaactctt ccaagtttgg atttctcata
     1261 taaatcggat tttgagctcc tttatattcc tcagtaaaat atggtccctc aaaatagatg
     1321 ccttgaattt ttgctccttg ttctttgccc gctactgccg caactgattt agacgcctct
     1381 tctagtcgct ttgtatctga agttagggtt gtcggcaaga aagatgtgac tccagtagat
     1441 agtaagccag cactcattcg caaaattcct tctgaatcac aatccataac atcagcgcca
     1501 gcaaaaccat gaatatgtgt atcaactaaa ccaggtgcta tttgataacc tgaataatct
     1561 aaaatctcct cttgtccaga aatattttct atccatttac caaagtgatt atcttttatt
     1621 tctaaataac cgttttcctt tacatgatca gcataaaaga atcggtcagc cttgatgtac
     1681 ttcgtcatct tcttcccccc attcactctc attataacgc tttaaggtat ttttgtaaat
     1741 ggtatatacc tattttttga caagtcatgg tttattctaa gaaagaactg tacacttgtt
     1801 ataaaccact tcacttggtt gtatttttaa ggttttaatt gctgttgctc ataattcttt
     1861 tccttcttca aaactatcaa ttgctaattc tcttatagtt tttgatgaga atggctaatt
     1921 aaaaagcata gcaaaaaagt ctagtaaaac tagactttca ggattttact gagcaacacc
     1981 tgtcccatta gtagcaggta cagtaccagt tcctggagtc ggaacagtat tgttaacccc
     2041 tggagtaact ggtgtattat tcgcttgcgg ttgctcataa gttggtgcta cattctgatt
     2101 aacgtaacta gaagtatcct gactagcagg attagctgtc ccttgttctg tagtttcagc
     2161 tactgaacta gagggagttt gattcccaat caaaccatat tgtgtcttaa cagtatcaat
     2221 acgtttttgt aaagcttctt tagattcttt gacagttaac ttattgactg ctgcttcagc
     2281 ttttaacaca ttttcttctg aaggggcttg ttctagaatt tctactaaat ctgtagcatc
     2341 tttggtagct ttcttatctg cttcgtcttt aagatactta tcttttgctt tcttaaaacg
     2401 tttttgcaaa gcttcttttt tagaagaatt ggacaattta tttaaagcaa gttcgacatc
     2461 cttcaataat ttttctgaag gtttcttctc aaatttatct aacatatctt gtgcctcagt
     2521 taattgagaa tcactgacat ggttaacact gctttctaca gtatccattt gagtagtgcc
     2581 atgatcttta aataagaaaa acgtaacaga agcaaaaatt aataaaataa tactagcaat
     2641 aagtgctaaa agttcttttt tatctatttt catactcact cctaaagttt cttcacatta
     2701 ttataacata aactaaagga cataaacaaa cctattttag aagaatcatc gtctatcact
     2761 cttattttct accggtaact tagttatatt tgacatctca actactttgt attacaatgt
     2821 agctatacca gtaacttgta ttacaatata gacaaggata taatatgatt ccatcgcaaa
     2881 tgctaaaagg attacttgaa ggagctatcc taaaaatcat acagcaagag gagacttatg
     2941 cttatgaaat ctcaaaaaaa ttaaattctt atggttttgg agaaatttcc gaagggacaa
     3001 tctaccccat tatcttaagg ctacaaagca ataaatatat aaaaggacaa caaaagcctt
     3061 cccctcaagg acctaagcgt aaatattata cgttaaccaa tgaaggcgtg gagcaattag
     3121 cccaatttaa acagaattgg gagtcactta gtaaagcatt aaacaaacta ctctaaaaat
     3181 tggagaggaa actagcatgg ataaactgaa aaaattacaa aaagaaaata cgctattaga
     3241 aggaagaatt gataactcta ataaccaaac ctatacagat atgattgtct atttgcgagg
     3301 tgctagcatt agcccctacc atcaggagct cattcgaaat gatattgtca atatgttatt
     3361 agaagcacaa gagcgacaag caagtttagt tagtgtattt ggagaagata ggcatgactt
     3421 tataaaccaa gttattaaat ctacacctaa aatctctaag aaagaagaaa cattacaacg
     3481 ttgggattta gccattttac tactaactat ccaaatgatt atctttttag gaggatatct
     3541 cattactgaa gcacttcaac agtcagtacc agatctaatc cctattactc tattagatgt
     3601 tctttttgca atctttatca gtattattgc tgtcaagata g
//
LOCUS       DATRSD010000123         3530 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRSD010000123 DATRSD010000000
VERSION     DATRSD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.124"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..1336
                     /locus_tag="VBD85_001881"
     CDS             <1..1336
                     /locus_tag="VBD85_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7134406.1"
                     /translation="HHFLAFRHLNVIYGVVIVLIILASLFLCIKNKARIFTTIILVLA
                     SIFVATTLYGFKSTIDLTNNLNKTASYSEIEMSVIVPKDSKITNIEAVSKLAAPVKND
                     TSNITDLIEHIKSEKGISITPQKTDSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPD
                     FKSQIKTIYTYKIKKKINRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNT
                     NTHKVLLTTTPRDAYVKIPDGGGNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARI
                     NFSSFLKLIDLLGGVTVYNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGD
                     NDRGRNQEKVIAAIINKLASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTG
                     QRFTVESQALTGHGSTGELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            1342..2073
                     /locus_tag="VBD85_001882"
     CDS             1342..2073
                     /locus_tag="VBD85_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HEO7134407.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            2082..2774
                     /locus_tag="VBD85_001883"
     CDS             2082..2774
                     /locus_tag="VBD85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO7134408.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKALGL
                     TSLGIVPDLNKL"
     gene            2785..3483
                     /locus_tag="VBD85_001884"
     CDS             2785..3483
                     /locus_tag="VBD85_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO7134409.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVSESVATYGDY
                     GDYGNYGKRDRKRK"
BASE COUNT         1293 a          518 c          601 g         1118 t
ORIGIN      
        1 tcaccatttt ttggcattta gacacctgaa cgtcatttat ggagttgtaa ttgttttaat
       61 cattttagca agtttatttc tttgtattaa gaataaagct agaattttta caactataat
      121 tttagtatta gcttctattt tcgttgctac tactttatat ggatttaagt caaccattga
      181 tttgacaaat aatctaaata aaactgcttc atactctgaa attgagatga gtgtaattgt
      241 accaaaagat tctaaaataa ccaatataga agctgtcagc aaattagccg caccagttaa
      301 aaacgatact tcaaatatta ctgatttgat agaacatata aaatcagaaa aaggaatctc
      361 tattacacca caaaaaacag attcttacca ggatgcatac aatagaatta aaaatggtga
      421 tagtcaggct atggttttaa ataatgctta tgttagctta attgaactta gcacccctga
      481 ttttaaatcg cagataaaaa cgatttatac ttacaaaatt aagaaaaaaa ttaatcgtaa
      541 aaatactaat cataaagaag gggtatttaa tatctatatt agcggtattg atacttttgg
      601 ctctatatca acagtatcaa gatctgatgt aaatattatt atgacggtta ataccaatac
      661 ccacaaagta ttgttaacga caacaccacg agatgcctat gtaaaaattc cagatggtgg
      721 gggcaatcaa tatgataaat taacccatgc aggtttgtat ggcgttgaga catcaatgaa
      781 aacacttgaa aacctttacg acatcaacct tgattattat gctagaatta atttttcatc
      841 atttttaaaa ttaatagacc tcttgggagg agtgacagtt tataacgatc aagcttttac
      901 aagtaaacat ggtaattttg acttccctgt tggtcaagta acattgaatt ctgagcaggc
      961 tttgggcttt gttagagaac gttattctct acaaggaggc gataacgata gaggtagaaa
     1021 tcaagaaaaa gtgattgcag ctattataaa taagttagct tctagtcagt cagtaacaaa
     1081 attaaatagc attacctcac agctccaaac gtccgttcaa actaatatga ctattgataa
     1141 tattaatgat ttgattaaca atcaattgtc aactggacaa cgcttcactg tcgagtcaca
     1201 agcattaact ggtcatggtt caacgggtga actcccttca tatgcaatgc caggagctca
     1261 actttatatg atgtcaattg atcaatctag cttatctaat gcaaaatcaa aaattaagaa
     1321 cacaatggag gaataacaag aatgattgat attcattctc atatcgtttt tgatgtagat
     1381 gatgggccta aaacgcttga agagagttta tctttaatag aagaaagtta tcgacaaggt
     1441 gttagaataa ttgtttcgac atcacataga agaaaaggta tgtttgaaac acctgaagat
     1501 attatcttca aaaacttttc tatagttaaa cacgaagcgg aaaaaagatt tgaacatctt
     1561 caaatcttat atggtggaga attgtactat acaagtgata tgttagaaaa actgaagtta
     1621 aagcaaattc caactttaaa caatacgaaa tttgctttaa ttgaattttc tatgcaaact
     1681 tcttggaaag atattcatac agctttgtca aatgttttaa tgcttggtat tacaccagtc
     1741 gttgcgcata tagagaggta taacgcttta gagaatcaaa aagaacgggt gaaggaaatt
     1801 attaatatgg ggtgttacac acaaataaat agttcccata ttttgaaaca aaaacttttt
     1861 aatgataagc ataaacgctt taagaaaaga gcccgttatt ttttagagga aaatttagtg
     1921 cattttgtag cgagtgatat gcataacctt gatgttagac cgccattttt agcagaagct
     1981 tataagatta tctgtagaga tttcggtaaa gaacgtgcta accaactttt tattgaaaat
     2041 gctcaatcta tattaaaaaa ccattacatt taggagattt catgaataaa atagctaata
     2101 cagaagttga aataaatata ttcaaccttt taaaaaaatt gtggaaaaag aaatttttaa
     2161 tcacatttgt tgccattgca tttgcaacag ctggactttt ctatagtctt tttattgtta
     2221 cgccacaata tacttcttca acaaggatat atgtcattaa ccctaatacc cctaataata
     2281 gtattactgc acaagatcta caagcgggga gttttcttgc aaatgactat aaggagatta
     2341 ttacgtctac tgacgttcta gaaaaagtta tttcttctga aaaattgaat tatccttcgt
     2401 ctcagttgtt gcaaaaaata acagtttcta ttttaaaaga tacacgtgtt atttcaatat
     2461 cggtcgaaga tgctaatcca aaaatgtctc aaaaattagc aaattcagtt agagaagcag
     2521 cagtttcaaa aatcaaggca gttactcaag tagaggatat cactactctt gagaagggaa
     2581 atttacctaa agcaccatct tctcctaata ttaaaaagaa tgtactaatc gggtttattg
     2641 ttggtgcagg attatcaact attgttttag ttattatggg tattttggat gatcgtgtta
     2701 atactgaaga agatattgaa aaggccctag gacttacttc tttaggtata gtaccagatt
     2761 tgaataaact ttaaggagaa tataatgact cgtttagaaa tagttgatag caagttaaga
     2821 caagcaaaaa aaacagaaga atacttcaat gcgatccgta caaacataca gtttagtgga
     2881 aaggaaaata aaattcttgc aattacctct gttagggaag gggaaggaaa atccactact
     2941 tcaacaagtt tagctttatc tttagctcaa gcaggattta aaacattatt aattgatgcg
     3001 gatactagga actctgttat gtctggaacc tttaaagcaa ctggaactat taaaggcttg
     3061 acgaattatt tatcaggtaa tgcagatctt ggagatatta tctgtgaaac caatgttcct
     3121 agactgatgg tcgttccttc agggaaagta ccaccaaatc caacagcatt acttcagaac
     3181 gcttatttta ataagatgat tgaagctatt aaaaatatat ttgattatat tatcatcgat
     3241 actccaccta ttggtttagt tgttgatgcc gcaataatcg ctaatgcttg cgatggtttt
     3301 attttagtaa cccaagcagg tagaataaaa cgtaattatg ttgaaaaagc aaaagaacag
     3361 atggaacaaa gtggttcaaa gttcttaggt attattctta ataaagttag tgaatctgtt
     3421 gctacttacg gcgattacgg cgattatgga aattacggaa aaagggatag aaaaaggaag
     3481 taaggggctc ttgtattgaa agaaaaagaa aatatacaaa agattattat
//
LOCUS       DATRSD010000124         3526 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRSD010000124 DATRSD010000000
VERSION     DATRSD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.125"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            1..3465
                     /gene="mfd"
                     /locus_tag="VBD85_001885"
     CDS             1..3465
                     /gene="mfd"
                     /locus_tag="VBD85_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO7134410.1"
                     /translation="MVRTWHSGLVTNSRQLVMGFSGASKAIAIASAYEKLSKKIMVVT
                     ATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRLSALRFLKDPEK
                     NGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLGYQKVSQVFSPG
                     EFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLESVQISPADDII
                     LQDADFERAKKKLEGYLVTASEVQRAYLSEVLSATENHFKHSDIRRFLSIFYEKEWGI
                     LDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLNYFSDPYKQLRQ
                     YQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRYKKAGATVLLQV
                     DSQKGLNLLQENLKEYGLDLIISDKNDIAPKESQLIVGHLSNGFYFADEKIVLITERE
                     IYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIETIEIQGIHRDY
                     LTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQRVAKQVEDIAD
                     DLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIKQDMEGNRPMDR
                     LLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKERFSNYPVTVDV
                     LSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVIDEEQRFGVKHKE
                     KLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQTYVLETNPGLV
                     REAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQMSEIQLENTLID
                     FINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRVGRSNRIAYAYL
                     MYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGASQSGFIDSVGF
                     EMYSQLLEQAIATKQGKSLIRQKGNAELALQIDAYLPAEYISDERQKIEIYKRIRELE
                     TRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERKGNEISILFEKA
                     SLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEELLKFAECFIKI
                     KSRKPVEE"
BASE COUNT         1221 a          509 c          687 g         1109 t
ORIGIN      
        1 gtggtgcgga catggcactc tggattagta actaatagtc gccaattagt tatggggttt
       61 tcaggagcaa gtaaagctat tgctatagca tcagcctacg agaagttatc taaaaaaatt
      121 atggtagtca ctgccactca aaacgattct gataaattat caagtgatat atcaagttta
      181 attggagaag ataacgttta tcaatttttt gctgatgacg ttcctgcagc agagtttatt
      241 ttctcttcat tagataaatc aatttcacga ctcagtgccc tgaggtttct aaaagatcca
      301 gaaaaaaacg gtgttttgat tactagtatc agtggtttaa ggttactatt accaaatccg
      361 gaagttttct cgaaaagtca atataagttt gaaatcggtc aagaatgtta tttagataaa
      421 ttgagtaaga acctagtcaa tttaggttat cagaaagttt ctcaagtatt tagtcctggt
      481 gaatttagcc aaagaggaga tattttagat atttttgaga tgacacaaga atatccttat
      541 cgcttggaat tcttcggaga tgaaatagat ggcatcaggc agtttgatat tgacactcaa
      601 aagtctctta aacaattaga gtcagtacag atttcgccag cagatgatat tattttacaa
      661 gatgcagatt tcgagcgtgc aaagaaaaaa cttgagggct atctagtaac agctagtgag
      721 gtacaacgcg cctatttgag cgaggtttta tctgcaactg aaaatcattt taagcactca
      781 gatattcgtc gttttctaag tatcttttat gaaaaagaat ggggaatatt agactatatt
      841 cctgagggaa ctccactttt tgttgatgat tttcaaaaaa ttgtagatcg aaatgctaag
      901 ttagatttag aaatagctag ccttctgacg gaagatttac aacaaggcaa gtctcattct
      961 agtttgaact atttttcaga tccttataag caattaagac aataccaacc agcaaccttc
     1021 ttttcaaatt tccacaaagg attaggaaat ctgaaatttg ataaacttca tcattttact
     1081 caatatggaa tgcaagaatt ttttaatcaa tttccattgt tggtcgatga aatcaatcgt
     1141 tataagaaag ctggagcgac agtactgttg caagtagatt ctcaaaaagg tttaaatctc
     1201 cttcaagaaa atttaaaaga atatgggtta gatttaatca tttcagataa aaacgatatc
     1261 gctccaaagg agtcacaact tattgttggg catctttcaa atggttttta ttttgcagat
     1321 gagaaaattg ttctgattac tgaacgtgag atttatcata ggcgtgttaa aaggaaaatt
     1381 agacgcagta atattagtaa tgcagaacgt ctaaaagatt ataatgaact ttcagttggc
     1441 gattatgttg ttcataatgt tcatggcgta ggaaaatttt taggtattga aacaattgag
     1501 atacaaggta ttcatcgtga ttatctgact attcaatatc aaaatgctga tcgtatctct
     1561 atccccgtag aacaaattga acttctaaca aaatatgtct ctgcagatgg caaagagccc
     1621 aaaattaata cgttaaatga tggtcgcttc aaaaaagcaa aacagagagt tgcaaagcaa
     1681 gtagaagata ttgctgatga tttgttgaaa ctttatgcag agcgtagtca attacaagga
     1741 tttgcatttt ctcctgacga taatatgcaa aatgactttg ataatgattt tgcatatgta
     1801 gagacagagg atcaattgcg gtcgataaaa gaaattaaac aggatatgga aggtaatcgt
     1861 ccgatggatc gattattggt tggagacgtt ggttttggta agacagaggt tgctatgcgt
     1921 gcggctttca aagctgtcaa tgaccataaa caagttgctg ttttagtccc aacaacagtt
     1981 ctagctcagc agcattttga aaattttaaa gaacgtttta gtaattatcc tgttacagtt
     2041 gatgtactta gtcgttttcg tagcaaaaaa gaacaaacag atacactaaa aagattatct
     2101 aagggccaag ttgatattat tattggaaca catcggcttc tctctcaaga tgttgtattt
     2161 tcggatttag ggttaatagt tattgatgag gaacagcgat ttggtgtaaa gcataaagaa
     2221 aagttaaaag aattaaaaac aaaagttgat gtcttaactt tgacagcgac cccaatacct
     2281 agaacattac atatgtcaat gcttggcatt agagacttat cagtcattga aacacctcca
     2341 actaatcgtt atcctgtgca gacttacgtt ttggagacaa atcctggttt agttagagaa
     2401 gctattattc gtgagataga cagaggagga caggtttttt atgtttacaa taaagttgac
     2461 actattgaac agaaagtttc agaacttcag gagttagtac cagaagctag tattggtttt
     2521 gttcatggtc aaatgtctga aattcaatta gaaaatacat taatagactt tatcaatgga
     2581 gattacgatg ttttagttgc aacgacaata attgagacag gagtcgatat ttcaaatgtt
     2641 aataccctct ttgttgaaaa tgcagatcat atgggcttgt caacccttta tcaattaaga
     2701 ggccgtgtgg gtagaagtaa tcgcattgct tatgcttacc ttatgtacag acctgataaa
     2761 gttttaacag aaatttctga gaaaagacta gatgcaatca aagggtttac agaattggga
     2821 tctggtttta aaatagcgat gcgtgattta tctatacgtg gtgcaggaaa tattttagga
     2881 gcttcacaaa gtggttttat tgattcggtt ggattcgaga tgtattcaca gcttctagag
     2941 caagcgattg ccaccaaaca agggaaatca ttaattcgtc aaaaaggtaa tgctgaatta
     3001 gccttacaaa ttgacgccta tttaccagca gaatatatat cagatgagcg ccaaaaaata
     3061 gaaatatata agcgtattcg tgaattagaa actagggctg attatgaagc cttacaagat
     3121 gagttaatag atcgttttgg agaatatcct gatcaagttg catacctttt agaaatagga
     3181 cttttaaaag cttatctgga ccttgccttt actgaattag tagaaagaaa aggtaatgaa
     3241 ataagcatcc tatttgaaaa agcatctttg aagtattttt taactcaaga ttattttgaa
     3301 gcactttcaa aaactcaact taaggctaga attagcgaaa caaatggtaa gatggaagtt
     3361 gtttttaata tcaaacataa gaaaaattat gaaattattg aagaacttct aaaatttgcc
     3421 gaatgtttta tcaagattaa gtcacgtaaa ccagtagaag aataactaag tgaaatgttg
     3481 caaggaaatt tggatactct tttagaaaat gtttggttca agaaga
//
LOCUS       DATRSD010000125         3485 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRSD010000125 DATRSD010000000
VERSION     DATRSD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.126"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..344)
                     /locus_tag="VBD85_001886"
     CDS             complement(<1..344)
                     /locus_tag="VBD85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7134411.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRPPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAK"
     gene            complement(357..812)
                     /locus_tag="VBD85_001887"
     CDS             complement(357..812)
                     /locus_tag="VBD85_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO7134412.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            1010..2317
                     /gene="eno"
                     /locus_tag="VBD85_001888"
     CDS             1010..2317
                     /gene="eno"
                     /locus_tag="VBD85_001888"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO7134413.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(2425..>3485)
                     /locus_tag="VBD85_001889"
     CDS             complement(2425..>3485)
                     /locus_tag="VBD85_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7134414.1"
                     /translation="RKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTKEN
                     GTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFYYN
                     DGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYEKQ
                     LKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQDQF
                     GISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKAAA
                     ETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRWHR
                     PNGQYASKAEIAAAGLQW"
BASE COUNT         1013 a          623 c          674 g         1175 t
ORIGIN      
        1 tattttgcga taaaatggtc tacaaagtaa gaacgctcag agaaccactc tctattaaga
       61 aaggttagac attgaggaac gataccagtt tcttctttga gttcccttag aatagcttgc
      121 ttagcatttt ctcctttaag ggcagatccg ccagcggtgg cttcgaaata agcaggaaaa
      181 gctttctttt tagggggccg tttcatcatc aatacgtcac catcctcatg aaaaaggata
      241 acatcaacaa ctaagtggaa taagccttgt gggatatttt gatccctatt aagtgtaaga
      301 cctgtaatga acccatattg gtcataagca tcccaaattt ccattagacc tccttgttat
      361 ttttgactaa agaaatgttg ccatagactt tttttaggag actcctcttt ttttggtgaa
      421 ttgaataaat cagggaattg tgtctcaaaa tcgttccatt caacgttttc agccttatca
      481 ctatgtataa taataccaaa tggagaattg gcatttgctt cattaacaat cgtagcagct
      541 agtgatagac ggttagctaa cttcatgtag aacatttgaa catcagtagg gagatttggt
      601 gataatttta aactaagatt aggatattca agtatcagat tattaagagc agatgttata
      661 gcaagttgaa acttttcttc ttgtgcctct tttaaactta agtggagcac aactctttct
      721 ttataagtac caaggtagta acgttgttga tcaggatcaa gacgattttc accgcttgct
      781 ttttgtagga ttttattgtc aagttcagtc ataatttctc cttgaatttg ctaattttat
      841 tataacctaa atgggttgca ttagcacagg taaacttgaa aagaattagt gattttttta
      901 tcaaaaacca cgtaataaag cggtttcact gggtttttcc ttgttattaa ctaaagatta
      961 tgatattatg aatatgtaaa aaattaaaac tcataaggag agtattgtaa tgtcaattat
     1021 tactgatgtt tacgcacgcg aagtccttga ctcacgcggt aacccaacac ttgaagtaga
     1081 agtttataca gaatcaggtg ctttcggacg tggtatggtt ccttcaggag cttctactgg
     1141 tgaacacgaa gcagttgaac ttcgcgacgg cgacaagtct cgttacggtg gtcttggaac
     1201 acaaaaagct gttgacaatg taaataacgt tattgcagaa gcaatcattg gttatgatgt
     1261 acgcgaccaa caagctatcg accgtgctat gatcgctctt gacggaactc ctaataaagg
     1321 taaattaggt gcaaatgcta ttcttggtgt ttctattgcc gtagcacgtg ctgcagctga
     1381 ttaccttgaa gtaccacttt acagctacct tggtggattc aacactaaag ttcttccaac
     1441 tccaatgatg aacatcatca acggtggttc tcactcagac gctccaatcg cattccaaga
     1501 attcatgatt atgccagttg gtgcaccaac tttcaaagaa gcacttcgtt ggggtgctga
     1561 agtattccat gctcttaaga aaatccttaa agaacgtggt cttgaaacag ctgttggtga
     1621 tgaaggtgga tttgcaccta aatttgaagg tactgaagat ggcgtagaaa ctattcttaa
     1681 agctattgaa gcagctggtt acgaagctgg cgaaaacgga atcatgattg gttttgactg
     1741 tgcttcatca gagttctacg atgcagaacg taaagtatac gactacagta aattcgaagg
     1801 tgaaggtgga gcagttcgta ctgctgcaga acaaatcgat taccttgaag agttggttaa
     1861 caaataccca atcatcacta ttgaagatgg tatggatgaa aacgactggg atggttggaa
     1921 agctcttaca gaacgccttg gtggacgtgt acaattagtt ggtgacgatt tcttcgttac
     1981 aaatactgat tacttagcac gtggtatcaa agaagaagct gctaactcaa tccttatcaa
     2041 agttaaccaa atcggtactt tgacagaaac tttcgaagct attgaaatgg ctaaagaagc
     2101 tggttacact gcagttgtat cacaccgttc aggtgaaact gaagattcaa caatcgctga
     2161 tatcgcagtt gcaactaacg ctggtcaaat taaaacaggt tcattatcac gtactgaccg
     2221 tattgctaaa tacaaccaat tacttcgtat cgaagaccaa cttggtgaag ttgctcaata
     2281 caaaggtatc aaatcattct acaacttaaa aaaatagtct cagattatta aacagttgaa
     2341 aatataataa aaaccatctt gcatatgcaa gatggttttt atgtacacat tataataata
     2401 gaattgattt gataataaac agttctacca ttgcaatcca gcagcagcga tttctgcttt
     2461 tgaagcgtat tgtccatttg gacgatgcca ccttcctcta tagtcacgtt tgtagccttg
     2521 actagtttgt tcttgtacta gagtttcttg ttttttctga gtttcagctg cgagccttgc
     2581 ttgctcctct ttttgcttcc tttgagcttc agcagcaagc cttgcttgtt cttcttccag
     2641 ttttttctga gtttcagcag cagctttttc ctcttcaact ttcttttctg caagtcgttt
     2701 ttcttcttcg tgcttagcga taagttttct ttgttcttta gctgataaga cagtattttg
     2761 agcctttccg ctgaggtagt caatggtagc atttggtgag gtattctcta aggtaacagt
     2821 gctgatgccg aattgatctt gagtggattt gtttccgata aaaataggta ataatttccc
     2881 agtcttatca ataccaacat attttaattc aatttttctt ggaattagtt cattattttg
     2941 ataaattggt gttactttat aatctaagta gtagttttta tgagtcgaaa gccaagtctt
     3001 tagttgtttt tcatagaata gcatgctctc agggttagat gaattcgtgc tattgtagtt
     3061 tccagtattt agccaattcg tcattggaac taaattcttt ctttcattgt ttagtccact
     3121 aaattggtgg caaataagac ggcctcggct cattagccaa gctttttgag taccatcatt
     3181 ataatagaat ttaaaattgt gccatccaac aggggttgtt tttaaacgtt ttactctctt
     3241 tttcttgggt tcatctttaa cttttagctg aatatgtgcg aatgtggcac gacctttgtc
     3301 atccaactct cctaaaacca attgtcgttt gtgtttaaaa ggtaaagtac cattctcttt
     3361 tgttttatac tcttgagcag taacaatatt ttctgtggta tgatttgtag ctaggtttat
     3421 tggtgtgtaa agcgaaataa ccgttagtaa ggttgcaatt cctaatttta taaactgttt
     3481 tcttt
//
LOCUS       DATRSD010000126         3469 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRSD010000126 DATRSD010000000
VERSION     DATRSD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.127"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..168)
                     /locus_tag="VBD85_001890"
     CDS             complement(<1..168)
                     /locus_tag="VBD85_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134415.1"
                     /translation="MYADKFYSGLFSQLNLNPDKSGIKPSGGFTMVPKDSTGEGYLWT
                     YPVNAFCSINIY"
     gene            433..1044
                     /locus_tag="VBD85_001891"
     CDS             433..1044
                     /locus_tag="VBD85_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEO7134416.1"
                     /translation="MTDKKGLNPNRLNGKDLINAGIFSAIYVVIIIAVACTLGLIPIG
                     FLLLTFVVPFVEGIPMMLYFTKIKKFGMLIILQAVIGIAMILTGMGYDLLIWAIITGI
                     VGELILKASKYQKSMGCVLAYAVSSICIAGNYVHWLTASQEWLKNKAASYGETFMYSV
                     YGWFHSNWWLFPLLIVSCFVGGFLGGMIGRKVLRKHFERSGLV"
     gene            1044..1787
                     /locus_tag="VBD85_001892"
     CDS             1044..1787
                     /locus_tag="VBD85_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7134417.1"
                     /translation="MISELYQKNATADKLYLDPRTKILMCLLVSTTLLSGSPVGYLKV
                     FHTILSLLPFVFLIVLKKVRAVVYFFALYLFALFVPDLLAPHLPTWFNLLFTGIIAFS
                     VEIIPGYMMAYFIFSTTSVGEFIAAMDRMHVTKKLTIPISVTFRFFPTIREEYVNVRD
                     AMKLRGIGSFRNPLMMLEFRMVPFLTSIVSIGNDLAASALTRGLAAPNERTNVSNIGF
                     TWRDYLVFGSLIIFYILYFILMFKQKGAL"
     gene            1784..3268
                     /locus_tag="VBD85_001893"
     CDS             1784..3268
                     /locus_tag="VBD85_001893"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7134418.1"
                     /translation="MIKFENASFVYKNGQRNNGMRDVSLNIPEGQVVLLCGESGCGKT
                     TLTRMINGLVPHFYEGEMSGEVFVCGNNVAECELYQLAYYVGSVFQNPKSQFYTVKTD
                     TEVVFTCENLGMEKQLIFERFENIVEALDITALLGKSLFSLSGGEKQRVACASAIAPN
                     PPIVVMDEPTSNLDTHTIAQLRKIIKKWKQDRKTVIIAEHRLEWLMDLADRVIYMKNG
                     RIINDYTIEQFKKISKEQLKIMGLRSKPEFQKENRATDNAGKIEFVDFSYTYKGENKP
                     ALSIPYINLPENSIIGIVGNNGAGKSTFGHAFCGLEKRSKGDIFLNGESFKRKNRIES
                     TYIVMQDVNQQLFTESVFEEVRLSMERLGLSHEEQEKRTNIILKELGLEEFKDSHPMS
                     LSGGQKQRVAMASALASDKRFLLYDEPTSGLDYMHMIEVADAIKRMKAARRTQFVITH
                     DSELVSRCCDYILFLECGEVKCIGNIDDMAIQETLQEFWNQVYM"
BASE COUNT         1108 a          467 c          730 g         1164 t
ORIGIN      
        1 gtaaatattt atagaacaaa atgcatttac agggtatgtc catagatatc cttcgccagt
       61 actatctttt ggcaccatgg taaaaccacc actgggtttg ataccagatt tatcaggatt
      121 tagatttagt tgagaaaata aaccggaata aaatttatca gcatacattt gcggcgctcc
      181 tttttataaa atgtccaaaa tttttagtat aatgtccaaa caaaatttgg agatattgca
      241 aaggataact ttgcatatta tcataaatat atgagttagc taggtctaac cttttgtata
      301 tattatatgc tgaaccacaa gggaaatcaa gtaaaatgag tttataaatt aataatatgt
      361 attggataaa atactaaatg atgtttcttg agtatcaagc gtaaatataa aaaattaagg
      421 aggatgtatt acatgacaga taagaaaggt ttgaatccaa acagattaaa tggtaaagat
      481 ttaatcaatg ctgggatttt ttcagcgatt tatgtggtta ttattattgc agtcgcatgc
      541 actttggggt tgattccaat tggcttttta cttctcactt ttgttgtccc atttgtagaa
      601 ggtattccaa tgatgcttta ttttacaaag ataaagaaat ttggaatgct aataatttta
      661 caggcagtaa ttgggattgc catgatttta acaggaatgg gatatgatct attgatttgg
      721 gcaatcataa caggaattgt tggagaactt attttgaaag cttccaaata ccaaaagagc
      781 atggggtgtg ttcttgcata tgcagtatcg agtatatgta ttgcgggaaa ttatgtacat
      841 tggctcacag cgagtcagga atggttaaag aataaggcag ctagctatgg agaaacattt
      901 atgtattctg tttatggatg gtttcatagt aactggtggt tattccctct tttgattgtt
      961 tcttgctttg tgggcggctt tcttggcggg atgattggtc gtaaggtttt gaggaaacat
     1021 tttgaaagaa gcgggttggt gtaatgattt ctgaactata tcagaaaaat gcaacggcgg
     1081 ataaactata tcttgatcca cgaacaaaga tattgatgtg tttgcttgta tcaactactt
     1141 tgctcagtgg ttctcctgtt ggatacttaa aagtttttca cacgatactt tcacttttgc
     1201 cgtttgtttt tttgattgtt ttaaagaagg tgcgagcagt agtttatttt tttgctttgt
     1261 atctttttgc actatttgtt ccagatcttc ttgcaccgca tttaccaact tggtttaacc
     1321 tcttgtttac gggaataatt gcattttcgg tagaaattat tcctggttat atgatggcat
     1381 attttatttt ttcaacaaca tctgtaggcg agtttattgc agctatggat agaatgcatg
     1441 ttacgaaaaa acttacaatt cctatttcgg taacatttcg tttttttcct accattagag
     1501 aagaatatgt aaatgtgcga gatgctatga aattgcgagg gataggttct tttcgcaacc
     1561 ctcttatgat gttagagttt aggatggttc catttttaac aagcattgta tctattggta
     1621 atgatttggc tgcttctgca cttacaaggg gacttgcagc acctaatgaa agaactaatg
     1681 taagtaatat tgggtttacg tggagagact atcttgtttt cggcagttta attatttttt
     1741 atatccttta ttttattctt atgttcaagc aaaagggggc tttatgatta aatttgaaaa
     1801 tgcaagtttt gtatataaga acgggcagag aaataatggt atgcgagatg tgagcttaaa
     1861 tattccggaa ggtcaagttg tcctgctttg tggtgaatct ggttgtggaa aaacaactct
     1921 tacaagaatg ataaatggtc tagttccgca tttttatgaa ggggaaatgt caggtgaagt
     1981 ttttgtttgc ggaaacaatg tagctgaatg tgaactgtat cagctagcgt attatgtagg
     2041 atcagtattt cagaatccaa aatcccagtt ttatacagtt aaaacagata cagaagtagt
     2101 attcacatgt gaaaatctcg gaatggagaa acaacttatt tttgaacgtt ttgaaaatat
     2161 tgttgaggct cttgatatta ctgcattgct agggaaaagt cttttttctc tatctggcgg
     2221 ggaaaaacag agggttgctt gtgcttccgc tatagcacct aatcctccaa tagtcgttat
     2281 ggacgagcct acttccaatt tagacacgca tactatcgca cagctgcgaa agattattaa
     2341 aaaatggaag caggatagaa aaacggtgat aattgctgaa catcgtttgg aatggcttat
     2401 ggatttagct gatcgtgtga tatatatgaa aaatggacga attataaatg attatacaat
     2461 agaacaattt aagaaaatca gtaaagaaca attaaaaatt atggggctta gaagtaagcc
     2521 tgaatttcaa aaagagaata gagcaacgga taatgctgga aaaatagagt ttgttgattt
     2581 tagttatact tacaaaggag aaaataaacc ggctctttct attccttata taaaccttcc
     2641 ggaaaactct attatcggca ttgttggaaa caatggagca ggtaaatcta ctttcggaca
     2701 tgctttctgt ggtttggaaa aaaggtcgaa aggggatatc tttctaaatg gagaaagttt
     2761 taaaagaaaa aatcgaatcg aaagtactta tattgttatg caggatgtaa atcagcagct
     2821 ttttaccgaa agtgtatttg aagaagtcag gttatctatg gagaggttag gattgtctca
     2881 tgaagaacag gagaaaagaa caaatattat tctgaaagag ttagggttgg aagaatttaa
     2941 agactctcat cccatgtctt tgtccggagg gcaaaaacaa cgtgttgcaa tggctagtgc
     3001 gcttgcatct gataaaagat ttctcctata tgatgaacca accagtggac ttgattatat
     3061 gcatatgatt gaggtggcag atgctataaa gcgtatgaag gcggcgagac gaacgcaatt
     3121 tgtgattacg catgattcgg agcttgtttc aagatgctgt gattatattc tatttcttga
     3181 atgtggggaa gtaaaatgta ttggaaatat agatgatatg gcaatacagg agacacttca
     3241 agaattttgg aatcaggttt acatgtagat gaaatgatga attaagcttt aggttctttt
     3301 ttaaaataat ggacaaaata aatcagcaac tttgattgtt tgttttgatt ttgcttttgc
     3361 aagaggtact aagatgcctc ttgcaaaaaa aataacataa ttgatagtac tattatcaat
     3421 gtacaagggg gaattttatg gcgaaccaag atattagcat tactcctag
//
LOCUS       DATRSD010000127         3431 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRSD010000127 DATRSD010000000
VERSION     DATRSD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.128"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            291..1313
                     /locus_tag="VBD85_001894"
     CDS             291..1313
                     /locus_tag="VBD85_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7134419.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGIILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            1684..2976
                     /locus_tag="VBD85_001895"
     CDS             1684..2976
                     /locus_tag="VBD85_001895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO7134420.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDGLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(2999..3292)
                     /locus_tag="VBD85_001896"
     CDS             complement(2999..3292)
                     /locus_tag="VBD85_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134421.1"
                     /translation="MILINLIALPICFLAELLAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHSINALLFLSFTVVLLISGKFLSLISIYLLKRKSKHQKV"
BASE COUNT         1066 a          538 c          674 g         1153 t
ORIGIN      
        1 tttaaaagat taatgtaata aaaaattatc gtaataaaaa aaaattaaga aaataaaaaa
       61 ttattattga ctatggttat gctttcaggt aaaatgtgat tatattagca actaaggagt
      121 aatccatgag ataagcctaa taattatgtc ggacttcttt attgcacctt atccagttcg
      181 tagtacaatt aagtttcagt attacctaaa ggcgccttta ttgaaattga agcagtagta
      241 cacagacact gagaataaaa aacttaacta tttttagcgg gagtatattt atgaatatca
      301 ttattgggac cagtctttta atacttgtat tagcgatttt cactttattt aattataaag
      361 caccctacgg aacaaaagca atgggagcat tggcatctgc ggcttgtgca tccttccttg
      421 tagaagcttt tcaagattct ttctttggta aagttttggg atttcaattt ttaagtgaag
      481 tgggtggtgc taatggttca ctttctggtg tagctgcagc tatattggtt actattgcta
      541 ttggtgtaac cccaggatac gcagttttaa ttggtttatc tgtttctgga acaggtatta
      601 ttcctggttt ccttgcagga tatttagtgg gatttcttgt taagtggatg gaaagaaata
      661 ttcctggtgg tttagatctt atttcaatta ttattattgg tgcaccatta acacgtcttg
      721 tggccaaact gttgacgccg cttattaata gcactttgct caccattgga gatatcttaa
      781 catcaggtgc tcatagcaac cctattttaa tggggattat acttggcggg acaattgtag
      841 tagtggcgac agcaccactt tcttctatgg cattgacagc tatgctagga ttaaccggaa
      901 tgcctatggc tataggagcc ttgtctgtct ttggttcgtc atttatgaat ggtgtacttt
      961 tccataaatt aaaacttgga agtcgtaaag ataatatagc ttttgctgtt gagcctctaa
     1021 ctcaagctga cgtgacttca gctaacccta ttccaatcta tgtcactaat tttgttggtg
     1081 gtgcagcttg tggtatttta attgccttga tgaaattagt taatgatact cctggaacag
     1141 cgacaccaat tgcaggattt gctgtcatgt ttgcctataa cccaatgata aaagtactaa
     1201 taaccgctct aggttgtatt atcctatctt tactagcagg ctattttgga ggcattgttt
     1261 ttaaagatta taaattagtt acaaaagaag agctacaggc tagagataac tagttacttc
     1321 atgaaaagct aatagtaaat atcattaata tagatatgat atctgacttg tgataactgt
     1381 tcttgataat ttaaaattca acgactctaa taaaaagatc gttgaatttt tttattgtta
     1441 agaatgctgg tatcagtaaa tttcaagatt tctttatcaa ctataatgtt agttttttac
     1501 cgttttttta cacttctaat aattattttt ttcttttttt ccgataatat ttgaggaatt
     1561 tggcaaaaaa aaacattttt tgctataata aggtgtcaga caaactcgca ggtgaaattc
     1621 ctgccatgtt tttccagaaa ggattgggta gtcctaaaaa ggctcactca gtgttaaata
     1681 aatatgactt cagttgttgt agtaggtacc caatggggtg acgaaggtaa aggtaaaatt
     1741 acagattttt tatctgctga cgcagaagta attgcacgtt atcaaggtgg tgataacgct
     1801 gggcatacta tcgttattga taacaaaaaa tttaaacttc atttaattcc ttcaggaatt
     1861 ttcttcaaag aaaaaatttc tgtcattggt aatggtgttg tcgttaatcc gaaatcatta
     1921 gttaaagagt tagcttatct tcacggagaa ggtgtaacaa cggataacct tcgtatttca
     1981 gatcgtgcgc atgttatctt gccataccac atcaagcttg accaactaca agaagatgct
     2041 aaaggtgata ataaaattgg aacaaccatc aaaggaattg ggccagctta catggacaag
     2101 gctgcgcgtg tcggtatccg tattgctgac cttcttgata gagaagtttt tgctgagcgt
     2161 ttgaaaatta atttagctga gaaaaatcgt ttatttgaaa aaatgtatga cagtacacct
     2221 cttgaatttg atgatatttt cgaagaatat tatgaatatg gtcaacaaat taagcaatat
     2281 gtgactgata catccgttat cttaaatgat gctcttgatg ccggtaaacg tgttcttttt
     2341 gaaggagcac aaggagttat gctcgacatt gatcaaggaa catacccatt tgtaacatct
     2401 tccaatccag tagcaggtgg tgtcacaatt ggttcgggag ttggaccaag taaaattaat
     2461 aaagtggtag gtgtatgtaa agcttacact agccgtgttg gcgatggacc attcccaaca
     2521 gaactttttg atgaggttgg tgaccgtatt cgtgagattg gtaaagagta tggtacaacg
     2581 actggtcgtc ctcgtcgcgt tggatggttt gattctgttg ttatgcgtca cagccgtcga
     2641 gtatcaggta ttactaacct ctctctgaat tcaattgatg ttctttcagg gcttgatacg
     2701 gtaaaaattt gtgtggctta tgaccttgat gggaaacgta ttgactatta cccagcaagc
     2761 cttgaacagc taaaacgttg taaaccaatc tatgaagaat taccgggctg gtctgaagat
     2821 attacagctt gccgtagctt agatggtctt ccagaaaatg cacgtaatta cgttcgccgt
     2881 gttggcgaat tggttggtgt tcgtatttct actttctcag tagggcctgg tcgtgaacaa
     2941 acaaacattc ttgagtctgt ttggtcaaat atttaatatt aaaagtctga ctgtacagtc
     3001 agactttttg atgtttactt tttcttttta atagatagat actaataaga cttaagaact
     3061 tcccagaaat caataaaact actgtaaatg ataaaaaaag aagtgcattg atactgtgaa
     3121 tcaaagtatg taataatact agcgatacaa ctgttagaat actaccagtc atcaataagc
     3181 gttccagagg atagtagcca tgttgcttat gcatttctaa ttttaatagt agaccagcta
     3241 acaattctgc taggaaacaa ataggtagag ctatcaaatt aataagaatc atagaccaac
     3301 caaatccttg acccaaacct atatatgata gactattttt gggaattgtt gacgtgtgaa
     3361 ttataaaatc aatcgtaagg ataatgtcta taagaagtag tagcgctaac agataatgta
     3421 gtaagaagat t
//
LOCUS       DATRSD010000128         3364 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRSD010000128 DATRSD010000000
VERSION     DATRSD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.129"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..400
                     /locus_tag="VBD85_001897"
     CDS             <1..400
                     /locus_tag="VBD85_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134422.1"
                     /translation="VENKVLREYLRWILRYNSLETSDDAKKFADENFFEEFDFVTEER
                     RPLVDQTTKINVKELLDSIIKSPKQFDNGHILHASIREIPGLLTVNSQVSKLFQTRVN
                     EVYLKQIDDLVMYQNIYINLIKPDELDSFL"
     gene            635..949
                     /locus_tag="VBD85_001898"
     CDS             635..949
                     /locus_tag="VBD85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134423.1"
                     /translation="MKTRNRKGGYLANTANEYIDSKQAIHCLSVELEPQIRFEEGQPT
                     GEIIAYKAWFSQKGLPPFMVKFENEVTLPAYMVMVQFENLQACEVGFNVYFKADNLKE
                     VK"
     gene            949..1452
                     /locus_tag="VBD85_001899"
     CDS             949..1452
                     /locus_tag="VBD85_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134424.1"
                     /translation="MTKKELSQYLLQSLNMGLGALMQGETSYTNSFDCKIMEEGFLFL
                     PRLPAGYIIDDELYQKIFLIANASLFPRYTLLKQNSAYFMALDTEDIHVQRGLFFPWK
                     EGVSERLIISDLEDFASSQKETLIPIMKNLSLDFNKVNHIAIAGNSGSGKSYALTYFL
                     SLLKGIS"
     gene            1516..2025
                     /locus_tag="VBD85_001900"
     CDS             1516..2025
                     /locus_tag="VBD85_001900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7134425.1"
                     /translation="MIHPQKNRSKSDFVSEINENLSSCLELIQQRQEILYENPDHEFD
                     HLTIVIDEVLALSEGVNKVIKESFFSLLSQIALLGRATKIHLLLVSQRFDHNTIPISV
                     REQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQILDNEHPYQVLPLLCP
                     TYYTKKGIL"
     gene            2775..3341
                     /locus_tag="VBD85_001901"
     CDS             2775..3341
                     /locus_tag="VBD85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7134426.1"
                     /translation="MSKDKRSNKWAFLLYQESVPKNYLEVLEELHIPFVLSPWHDKDV
                     NKETGEFKKAHKHGALFFESLKSYSQVSELLTKHLNTPSHVEVIMSPKGMYDYFIHAE
                     NPDKTLYDINDIESGCGFELDKFLTSNNNDKFLSLVIDIIEEQNFTEFNNLVRYARSE
                     NPQLLALIMNKTYFFAKYLDSRRYSRRK"
BASE COUNT         1154 a          596 c          602 g         1012 t
ORIGIN      
        1 agtagagaat aaagttttaa gagaatattt aagatggata ttaagatata acagcttaga
       61 aacatctgat gatgcaaaga aatttgctga tgaaaatttt tttgaagaat ttgattttgt
      121 tactgaagaa aggcgtccat tagttgacca aaccactaaa ataaatgtaa aagagttgtt
      181 agattctatt ataaagtccc caaaacaatt tgataatggt catattttac atgctagtat
      241 aagagaaatt ccaggactat taacagtcaa tagtcaggta tcaaaattat ttcaaacaag
      301 agttaatgaa gtttacttaa aacaaataga tgatttagtc atgtatcaaa acatatatat
      361 caacttaatc aaaccagatg aactagattc atttttataa ccaaattatt aaacggacag
      421 ttacaggagt acttgtccgt tttcattttg tccataaaag aagtagaaac tgagaactaa
      481 agtaagaatt tagaagaatg tattctccga tatactaaag acatcaaagc acagcaaatc
      541 tttggtagtc aacgctcaag agagttgcaa atctgttgag tcacttttca ggaagttgct
      601 gattcctgaa cgggttttta atggaggata aaacatgaag acaagaaatc gtaaaggtgg
      661 ttatttagcg aatactgcaa atgagtacat cgactctaaa caggcaattc attgcttgag
      721 tgtagaactt gaaccgcaaa ttaggtttga agagggtcag cctactgggg agattatcgc
      781 ttataaggct tggttctctc aaaaagggct tccgcctttt atggtgaagt ttgaaaatga
      841 agtgacacta ccagcatata tggtaatggt gcaatttgag aatcttcaag cttgtgaggt
      901 tgggttcaat gtttatttca aggcagacaa tctcaaggag gtcaaataat gaccaagaag
      961 gaattgtcac agtatctgct tcaaagtctc aatatgggac ttggagctct tatgcaaggg
     1021 gaaacaagtt ataccaatag ttttgactgc aaaattatgg aagaaggatt tctctttctt
     1081 ccccgcttac cagcgggata tattattgat gatgaactct atcaaaaaat attcctaatc
     1141 gccaatgctt cactcttccc tcgttacaca ctattgaaac aaaattctgc ctatttcatg
     1201 gcacttgata cagaagatat tcatgttcaa cgtgggctat ttttcccatg gaaagaaggc
     1261 gtatctgaac gcttaatcat ttctgacttg gaagatttcg caagctctca aaaggaaact
     1321 ctgattccta tcatgaaaaa tctatctctt gattttaaca aggtcaatca tattgccatt
     1381 gcaggaaatt caggatcagg taaatcttat gctctgacct actttctgag tctcttaaaa
     1441 gggatttctt aactgattat cgttgaccct aaatatgata ctccaagcag atgggcaaga
     1501 gaaaatggta ttgcagtgat tcaccctcaa aagaatcgct ctaagtctga ttttgtatcg
     1561 gaaatcaatg agaatctaag ttcgtgctta gagcttatcc aacaacgcca agaaattctc
     1621 tatgaaaatc ctgaccacga atttgaccat ctaaccattg tcatcgatga agttttggca
     1681 ctttcagaag gagtgaacaa agttatcaaa gaatcctttt tctcactact ttctcaaata
     1741 gctttacttg gtagagctac taaaattcac ttactactgg tatcacaacg atttgaccat
     1801 aacacgattc cgatttccgt acgtgaacag ttaaatgtct tgattcaaat tggcaacatc
     1861 aataaaaaaa cgactcagtt cctctttcct gacttagacc ctgaaggtat tgtcattcca
     1921 ataggaaaag gaactggctt aattcaaata cttgataacg aacatcctta tcaagtattg
     1981 ccactccttt gtccaaccta ctatactaag aaaggtattt tataaaatga aaagaagttt
     2041 cttccaaaaa acgtttaacc tgaatctgaa tttgcttttc tttggtttca ttgccttctt
     2101 aatcagttat ggattccatg tactaggcaa tcaaatcaag acaagttctt tcttgagctc
     2161 tcaatcagtc tttgcagata ttcaaactaa gttacaagtg gtagaccagt atactcagtc
     2221 aatagcatat ttgtttattc tgattgccat tattctaacc ttgactgaac tctgtcagcg
     2281 aattatgaag gactcaatat ggaactattt taagtctgtt tatcaaacct tatgattgag
     2341 acaattctta gacaggaaga acattctgaa cctgttatga caatagaaaa tcaaacggtc
     2401 actcgcttta atcctatcct aagaagtttc aatcgggttg tttctaagtg cacggtggat
     2461 gcccgaaagg attctgtttc tgtctttcta aaagtcccta aaacgcaaca ggcacaaaaa
     2521 ctcttgcgag aaatggaaga acatatcaag gaagaaattt cgagccgaaa tcccaagtat
     2581 tatttctcta ccccaaatcg ggagaaaagt aagctctggt ttacaggtac taaacggtaa
     2641 gggaaagagt ggtgcttggc tcacgaagtg aaagccaaaa gcaccactct ttcttataac
     2701 tgtttttaac atgtgtaatg gggttactac gcccccccat tacaccaata atcaataatc
     2761 acgaaaggaa gtatatgtca aaagacaaac gctcgaataa atgggctttt ctgctctatc
     2821 aggaaagtgt gcctaagaac tatttagagg tgcttgaaga gttacatatc ccctttgttt
     2881 taagtccgtg gcatgataaa gacgtaaaca aggaaacagg ggaattcaaa aaagctcaca
     2941 aacatggggc tctcttcttt gaatctctca aaagctattc acaagtctct gaattattga
     3001 cgaagcacct aaatacacca agtcatgttg aagttatcat gtcacctaaa ggaatgtatg
     3061 actactttat tcatgcggag aatccagata aaacactcta tgatatcaat gacattgaat
     3121 caggttgtgg atttgagtta gataaatttc tcaccagcaa taacaatgat aaatttttat
     3181 ctctggtaat tgacattatc gaagaacaaa atttcacaga gtttaataat ctggttagat
     3241 atgcgagaag tgaaaatcct caactgcttg ccttgataat gaataaaacc tatttctttg
     3301 caaaatatct ggattcgaga agatattcaa ggaggaagta acgattgaca agaacaataa
     3361 atca
//
LOCUS       DATRSD010000129         3327 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRSD010000129 DATRSD010000000
VERSION     DATRSD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.130"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..219)
                     /locus_tag="VBD85_001902"
     CDS             complement(<1..219)
                     /locus_tag="VBD85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO7134427.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGY"
     gene            complement(248..1468)
                     /gene="pepT"
                     /locus_tag="VBD85_001903"
     CDS             complement(248..1468)
                     /gene="pepT"
                     /locus_tag="VBD85_001903"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO7134428.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAMDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKHLAQDIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            complement(1604..3238)
                     /locus_tag="VBD85_001904"
     CDS             complement(1604..3238)
                     /locus_tag="VBD85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7134429.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYAWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALIGKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
BASE COUNT         1128 a          648 c          513 g         1038 t
ORIGIN      
        1 atatccatct tcttcaataa caaaatagcg atgaaatcca atgcctacta aaacaataaa
       61 gatggcaaat agaacaaata cctgactcgg aatctcagtt ctttcatata gcagagataa
      121 accaataaaa attggtgcaa tggctattga ccaataaata atagcccaag aaagttcagg
      181 ttgccagtga taacgtatct tcccaaataa ttttatcatt taaccactcc aatctgatat
      241 ttctttacta atccttagca acgatgccta aaataacatc aactgctttt tccattgttt
      301 gtagactaac gaattcaaaa cgtccatgca tgttttcacc acctgcaaaa agattaggag
      361 tagggattcc cataaaggaa attttagagc catctgtacc accacgaatc ggttctatga
      421 ttggcttgat gtctaactct tccattactt ctttagcaat gttgataggt gtcatgtctt
      481 tttcaataac tttcttcatg ttgtaatatt gatcgtatag tttaaccttt acacgttcag
      541 attgtaatgc ttcattcata ttatcagcga tatcttgagc caagtgctta cgttttaaga
      601 aattaacttc ctcaaaatct cgaatgatat atgaactttt agcttgatca actgttccac
      661 ttaaatcata taagtgataa aatccttgat agccatctgt ttgttcagga cgttcatttt
      721 ctggtaattg actatgaaaa tccatagcaa gctgtaaagc attaatcatt tgattttttg
      781 cagttccagg gtgaacgttt cgtccttcaa atgtaagctc caaaccagct gcactaaagg
      841 tttcatactg taattctcct agtggtccac catccactgt ataggcaaaa tcaacatcaa
      901 aatctttaac atcaaattta tctgcaccta taccaatttc ttcgtctggt ccaaagccaa
      961 ctctaatttc acagtgctca atttctggat gcgaagccaa atattccata gctgtcatga
     1021 tttctgctat accagactta tcgtctgctc ccagtaaggt tttaccatct gttgtaatca
     1081 atgtttgccc atggtaatta tttaaatttg gaaaatcttt tggacttaaa atgtaacctg
     1141 agtctccaag ttcaatatct ccacctttat agtcaacaat ttgtggagta atattttcag
     1201 cattgaaatc agctgtatcc atatgggata taaaacctat tttatggcgt aagcggtcgc
     1261 ttgtagcagg taaggttcca accaaatacc cattagaagg taaataatga acatctttta
     1321 aaccaattgc ttccatttct ggttttaaaa ctgttaaagc aaagtcaact tgactttgag
     1381 ttgttggcgt ttgtgtacta ttaggattac ttcttgtatt tattttgacg tatgttaaaa
     1441 atcgttctaa aagcttttcg taagacatct ttcacctcat atttctccat ttaattataa
     1501 cataaaaata agctcactat attgtgacta ccttaaaatt tcacaaataa ttttggtcgt
     1561 caaaaaacta ggttaaacaa ctctgtctaa cctagttttt tcattagtta aggtgcaact
     1621 ttcggcgtaa gtcttgagca cgactgccaa tcatcttatc aagtagatgg gtggccagtg
     1681 ccataaagcc atatacccct aaacctattg caccgataac gataatgtaa actagactta
     1741 ccagtctact atcgggtaca ataaccaggt tcattaacca tattatcata gaaataataa
     1801 agctcatcaa tagcgttaga ataaggacta aagcacttgt acgacgaata gcttggcgtt
     1861 taaatcctgt aatttcatgt attttcaaat acataagaat tactgggata caaagagcta
     1921 ctgtcgttga gaaaagtggt ccataggcat ggaaaaggaa aatggaggga acttgtaaaa
     1981 taacttttgc caccagacca tataggaagt aaataatagc tttacgattt tcaaacaatg
     2041 cttgtagcat tggtgctaaa acagtataaa tagataaaat aattgtttga ataagagata
     2101 tgacaaaaag ccctaaagct tgtccctgcg gtaacccata aaagactgta tacaaaggtt
     2161 ttgctaaaat aacggatcct gcaacagctg gcagtaaaaa cattagtaac atttgtaaat
     2221 tattaacaac caaacgagcg gcggcttttt tatcattctt aacaaaattc tctgttaaca
     2281 atggaatacc aactccagca attgcggtag caacagcaat tagaatcatc gtaactttac
     2341 cagggttgct tgaaaagtaa gcaaacataa cccgtaattc ttcacttgaa taattcgtaa
     2401 agagcgccat cgtattccca aatgaaaact gatcaatcaa tttgaaaatt tgaattgctg
     2461 caccagtaat aataaaaggg attgcttcct taacagtctc aattagtatt tctttagtat
     2521 ctaatttaat gtgctctgga gttttaccaa tcaaagcaga tagcatatta taacgccata
     2581 agaaataaag cagcacagct atactagcaa acataccgac aaaggcggcg aatgttgact
     2641 gtgtcacagc ggcaatataa tctcctgatc ctagacgcat aatatagaaa gcagttaata
     2701 gcatccatat gacacgtata atctgctcag cgacttgact aatggcatac ggttttaaat
     2761 tattaaaacc ttgaaaaaaa ccacgtaaaa cactcattga aggaaaaact aaaacagcta
     2821 gagttagact acgcaatatt ggaacgagtt cttgccctcc tttactaagt gaagcaaata
     2881 gaggtgaacc aatatacata attaatgcaa aaactccacc taaaatcagc ataaactgca
     2941 agattttccg aaccaggtaa atgctcatct cttctttacc taaagtgtta tacttagata
     3001 cctgttttgc aactgctact ggaattccaa cagttgatat caaaagaaat aaagcataga
     3061 tttcatatcc catcccaaat aaagcatttg cttcggcagc atgctttccc atccaagcat
     3121 accaagggat aatatagatg gctcccagca accgactaat gaaattccct gctgttaacc
     3181 aagccgtccc tttgaccatt tgttcttgct gactaacttt tgttgttttt tgtgacatgg
     3241 taaactttct attttcaata ttatttagca tttaaaagac aaaaatctca gaaatatcac
     3301 tttattataa acttttacct tagactt
//
LOCUS       DATRSD010000130         3260 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRSD010000130 DATRSD010000000
VERSION     DATRSD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.131"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            237..872
                     /locus_tag="VBD85_001905"
     CDS             237..872
                     /locus_tag="VBD85_001905"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO7134430.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            892..1755
                     /locus_tag="VBD85_001906"
     CDS             892..1755
                     /locus_tag="VBD85_001906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO7134431.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKTETIKELTKDFSRSGFE
                     GKSQVFIIKDCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            1785..2111
                     /gene="yabA"
                     /locus_tag="VBD85_001907"
     CDS             1785..2111
                     /gene="yabA"
                     /locus_tag="VBD85_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO7134432.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            2111..2980
                     /gene="rsmI"
                     /locus_tag="VBD85_001908"
     CDS             2111..2980
                     /gene="rsmI"
                     /locus_tag="VBD85_001908"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO7134433.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
BASE COUNT         1124 a          517 c          628 g          991 t
ORIGIN      
        1 agagagttag gtgtttgtgt tttgtcatga tggataaact catttctaac ccaaacgtct
       61 cagacttaat tccaccataa aaatcctttt cacactaact tccggttttg gagggcagac
      121 ggaacttact ttgagaaatt actcttaaaa atatcagaca tgcacaatca agttgtgggt
      181 gtcttttttt gataaatttt gatagaatag tagtaattat aaataaagga tgtaccatga
      241 aaaaaggact aatgatttct tttgagggcc cagatggtgc gggtaaaaca accgttttag
      301 aagctgtttt acccttgctc agagaaaaat tatcacaaga tattctcacc accagagagc
      361 ctggtggcgt aactatttca gaagaaattc gtcatattat tttagatgtt aaacacacgc
      421 aaatggataa aaaaacagag ctattgcttt acatggctgc tcgacgccaa catctagtgg
      481 aaaaggtact cccagctcta gaagaaggga aaatagtcct aatggatcgt tttattgata
      541 gttctgttgc ctatcaggga tcaggaagag gattagataa gtcacatata aaatggctta
      601 atgactatgc gactgatagt cataaacctg atttaacttt atattttgat gttccttctg
      661 aagtaggttt agagcgtatt caaaaaagtg ttcaaaggga ggtaaatcgt ctagatctcg
      721 agcaattaga tatgcatcaa cgcgtccgtc aaggttacct tgaattggcg gattcggagc
      781 caaaccgcat tgtgacgatt gatgcctccc aacagctaga tgaagtaata gctgagacat
      841 tctctattat tttagatcgt atcaaccaat gaatcaggaa gtacaaaggt catggattta
      901 aagcgaacac aacccaaatt gctggaaaag tttaatacta ttttacagtc agataggatg
      961 agtcatgcct atcttttttc gggaaatttt gcaagtctag atatggcact ttatttagca
     1021 caaagtcagt tctgtgaaaa gcgccagagt ggtttaccat gtcaagagtg tagagcctgt
     1081 cgtttaatcg ctaatgggga attttcagat gttaaaatca tagaaccaca ggggcaactt
     1141 attaagacag agactataaa agagctgact aaagattttt caagatcagg tttcgaaggg
     1201 aaatcacagg tctttatcat caaagattgc gaaaaaatgc atgtcaatgc agccaacagt
     1261 cttttgaaat ttattgaaga acctcaaagt tcatcttatg tcatcttatt gactaatgat
     1321 gaaaacaatg ttttgccaac aattaagagt agaacgcaaa tttttcgttt tccaaaacaa
     1381 ttggacatgt tggttcatca agctgaacaa gcaggattgc taaaatcaca agctagctta
     1441 ttagctcaag tagcagacga tcctaaacat ttagaaatat tgttgactaa caaaaagtta
     1501 ttagattact tgaatttaag tcaacagttt gtgacgactt tggcaaaaga taggcaaact
     1561 gcttatttag aagtatctcg cttgacatcg caagttgtag ataaaaatga tcaggcattt
     1621 gtttttcaat ggctgacaat aatgctggct aaagaaggcc aactttatga tttagaaaat
     1681 acttatagag ctcagcaaat gtggaagagc aatgttagtt ttcaaaatag tttagagtat
     1741 atggtgcttt cttagaaaat aatacgcgca gagaggaaga gtgaatggat aaaaaagact
     1801 tgtttgatgc ctttgatgat ttttcacaaa atttattagt tgggttgtct gaaattgaga
     1861 ctatgaaaaa acaaatccaa aaattattgg aggagaatac tgttctacgc attgaaaatg
     1921 gtaaacttcg tgagcgtctg agcgttattg aagcagaaac agaaactgca gttaaaaatt
     1981 ctaaacaagg aagagaatta ctcgaaggca tttacaatga tggctttcac atttgtaata
     2041 ctttctatgg tcaacgtcgt gaaaatgacg aagaatgtgc tttttgtatt gaattattat
     2101 atagagattg atggaaatgc aagttcaaaa aagttttaaa tcaaatatac attacggaac
     2161 actctatcta gtcccaactc caattggtaa tctagatgat atgacttttc gtgccattag
     2221 gattttaaga gaagttgatt ttatttgtgc agaggataca cgaaatacgg gacttttact
     2281 caagcacttt gatattacta ctaaacaaat tagttttcac gaacacaatg cttacgataa
     2341 aatctctggg ttaattgatt tgttaaaaga agggaaatct ttagcccaag tatctgatgc
     2401 aggaatgccc tctatttctg acccaggaca tgaccttgtc aaggctgcta ttgaagggga
     2461 tatcccagtt gtatctatac caggagctag cgctggtatt actgctctca tcgcttcagg
     2521 tttagctcca caacctcata ttttttatgg cttcttacca cgtaagaaag gtcaacaaat
     2581 aactttcttt gaaacaaagc aagattaccc tgaaacacaa atcttttatg agtcaccgtt
     2641 tcgagtctct gatacgctaa aacacatgaa agagatttac ggagatcgcc aagttgtttt
     2701 agtacgcgaa ttgacgaaac tctatgaaga gtatcaaaga ggaaccatta gtcaactttt
     2761 agagcatatt gaaaaggtcc ctctcaaagg tgaatgctta attattgttg atggtaagag
     2821 agataccgag cgagtgaaag acagtagcca acaagatcca ctagtattag taaaagaata
     2881 tatcgctaat ggtgataaaa ctaatcaagc gataaaaaaa gtagcaaaag aatttaatct
     2941 caatagacaa gaactctatg ctagtttcca tgatttataa gtaataatat aaaaaggttg
     3001 ccaaagaaaa atttggcaac cttttataat ctccgattta gctattggaa taaagaagta
     3061 agatacttcc aatttcctta aaatttttaa tttcagctct tttgaatacc attgcaaatt
     3121 ggattttatt tctgagagta ggttataaaa gatggtatgc tcttattccg gttctttcag
     3181 gttttagaaa acatgagaga acttagtagt aaatatacta aagaaactta tagccctgtt
     3241 tcgaattttt tattgagtta
//
LOCUS       DATRSD010000131         3241 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRSD010000131 DATRSD010000000
VERSION     DATRSD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.132"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            16..1200
                     /locus_tag="VBD85_001909"
     CDS             16..1200
                     /locus_tag="VBD85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO7134434.1"
                     /translation="MKNNVICLAGDNKSLNQIQTVIKSILCHNDRVSIYILNQDIASE
                     WFRNIQRRLLNSHSCIFDIKLFDDTFKEFKTPRAHITYMAYARYYIPQLIDAEKVLYL
                     DIDTLVVDNLDKLFEIELGDYPIAAILDGDGIHFNSGVMLINSLYWMRYRVTEKLLEI
                     TERELDNGIFGDQGVLNLLFDNNWLKLEDKYNAQVGNDLGAFYENWQGYFDRNFESPT
                     IIHYCTHDKPWNTFSSSRFRETWWQYEQLDWNEVFNFETYLLPEPTFEKHFFTFTTSV
                     DLLYIEELVELFPNSCFHIAAWTSFGPRLLKLATNSNVRLYPSITSPLFEQLMTKANY
                     YLDISTSWKEIQFCQKAIEKSIPILSFNEAVTFEYRELSHCFINLEDMRQFLCKNGGN
                     SD"
     gene            1193..2068
                     /locus_tag="VBD85_001910"
     CDS             1193..2068
                     /locus_tag="VBD85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7134435.1"
                     /translation="MTDKLVSVIIPVYNAAPYLEGCVNTILGQTYQVFEILLIDDGST
                     DTSASICDQLSLRDNRIRVFHIENGGASKARNFGLARISPESQFVTFVDSDDWVKENY
                     LEVLLAQQEKYNADIVISNYYIYRETEDIFGYYITDKDFVIEEISAQTAIDRQVHWHL
                     NSSVFIVIWGKLYRRELFDTITFPIDKVFEDELVSVLLFIKSKKTILVNGSYYGYRIR
                     PNSIMTSAFSSKRAEDLIYIFDSKLSEIILSGLDSTATVERYFWLMRDYKQRLEQENL
                     QKTIIYKVISNRLSR"
     gene            2129..>3241
                     /locus_tag="VBD85_001911"
     CDS             2129..>3241
                     /locus_tag="VBD85_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO7134436.1"
                     /translation="MRVIKVKTISETLDYISKHTASVARFGDGEIDIIAGNSIPYQVY
                     DSELAKRLERLLNRQSNEKLVICVSDVFDNLGRYNDFSQQFWRDHLYQYNDLYQKNCQ
                     AEWYGSTFISRPYMDLLDKSLSVTYFEQLKALWKNKDILIVEGETSRSGVGNDLFNDA
                     NTISRIICPSKDAFSSFYEIKETILEHSRGKLILLMLGPTAKLLAEDLSQLGHQAIDL
                     GHIDSEYEWFKMGAVSKVKLSHKHTAEHNFDEDITLIEDNSYENQIVSKIGTSDRQYA
                     LTVTLTDDIWELEHLLQRCPNTDFHIAAPVYCSDRLKQLVGYPNYYLHEAITEEQFEV
                     LLLNSDIYLDINHGEEVWNVVDRCITEGKTIYGIRRA"
BASE COUNT         1092 a          446 c          615 g         1088 t
ORIGIN      
        1 ttagaaagga atattgtgaa aaataacgtt atttgtttgg caggtgataa taaatcttta
       61 aatcagatcc aaacagttat aaaatcgatt ttatgtcaca atgatagagt aagtatttat
      121 attttaaatc aagatatagc ttcagaatgg tttagaaata ttcaaagacg tttattaaat
      181 agtcatagtt gtatttttga tataaaactc tttgatgata cttttaagga atttaaaacg
      241 ccccgtgccc atattaccta tatggcttat gcaaggtact acattccgca actgattgat
      301 gcagagaaag ttctttattt agatattgat actttggttg ttgataactt agataagttg
      361 tttgagatag aattagggga ctacccgata gcagctatct tagatggaga cggtattcat
      421 tttaactcag gtgttatgct aataaactcg ctttactgga tgagatatcg agtaacagaa
      481 aaacttctgg aaataacaga acgagaattg gataatggaa tttttggaga ccaaggggtt
      541 ctcaatcttc tttttgataa taattggctt aagttagaag ataagtataa tgctcaagtt
      601 ggaaatgatt taggtgcctt ttatgaaaat tggcaagggt attttgatcg taactttgaa
      661 tcgcctacta ttatccatta ctgtactcat gataaaccat ggaatacttt ttcttcttca
      721 aggtttaggg agacatggtg gcaatatgag caactagatt ggaatgaggt gtttaatttt
      781 gaaacgtatc tgttacctga gccaacattt gaaaaacact tctttacttt cacaacgtcc
      841 gtggatttgc tttatattga agaattggtt gaactattcc ccaatagttg cttccatata
      901 gctgcttgga cctcttttgg cccaagatta cttaagttag ccacaaactc taacgttcgt
      961 ctgtatcctt caataaccag cccacttttt gaacaactaa tgactaaagc aaattattac
     1021 cttgatatct caacttcttg gaaggaaata caattttgtc aaaaagctat tgagaaatca
     1081 attccgatat tgagttttaa tgaagcagtg acatttgaat atagggagtt aagtcattgc
     1141 tttattaatt tggaggatat gcgacagttt ttatgtaaaa atggaggcaa tagtgactga
     1201 taaattagtt tctgttatta ttccagtcta taatgcagca ccatatttag aggggtgtgt
     1261 aaataccatt ttaggacaaa cataccaagt ttttgaaatt ctactgatag atgacggttc
     1321 tacagataca tctgctagta tctgcgatca attatcccta agagataacc gtattagagt
     1381 tttccatatc gaaaatggcg gtgcttctaa agctagaaac tttggattag cacgaattag
     1441 tccagagagc caatttgtta cgtttgttga ttcggacgat tgggtaaagg aaaattattt
     1501 ggaagtactg ctagctcagc aagaaaaata taatgctgat attgttatca gtaattacta
     1561 tatctacaga gaaacagaag atatttttgg ttactacatc actgacaaag attttgttat
     1621 cgaagaaatc tctgctcaaa cagctattga tagacaagtg cattggcatt taaatagttc
     1681 agtattcatc gttatttggg ggaaattata tcgaagagaa ctatttgata ccatcacctt
     1741 cccaatagat aaagtatttg aagatgagtt ggtaagtgtt ttacttttta taaaaagtaa
     1801 aaaaacaatt ttagttaatg gaagctatta tggctataga attcgtccaa atagcattat
     1861 gacatcggcg ttttcatcca aaagagcaga ggatcttatt tatatttttg atagtaaatt
     1921 gtcggagatt atactatcag gattggattc aactgcaact gtagagagat atttttggtt
     1981 aatgcgtgac tataaacagc gcttagaaca agaaaattta cagaaaacaa taatatataa
     2041 agtgatatca aatcggttat caaggtaaca gtaaatacca ttttatttgt cgtaatcttg
     2101 caaagagagt tgaaaagaat taatagttat gagagttatt aaagtaaaaa caatttcgga
     2161 aactttagat tatatttcta aacataccgc ttcggtagcg cgttttggtg atggagaaat
     2221 tgatattatt gcggggaatt ctattcctta ccaggtttat gattctgaac ttgctaagcg
     2281 attagaacgt ttattaaatc gacagagtaa tgagaagtta gttatctgtg taagtgatgt
     2341 ttttgataat ttaggtagat acaacgattt ttcacaacaa ttttggaggg atcatctata
     2401 tcagtataat gatttgtacc aaaaaaattg tcaggctgaa tggtatggtt caacttttat
     2461 ttctagaccc tatatggatt tactggataa gagtttgtca gtaacgtact ttgaacagct
     2521 aaaagcatta tggaaaaata aagatatttt aattgtcgag ggtgagactt ctcgttcagg
     2581 cgttggcaat gacttattta atgatgccaa tactatttcg cgtatcattt gtccttcgaa
     2641 agatgcattt tcatcttttt atgaaatcaa ggaaactata cttgaacata gtagaggtaa
     2701 gctaatttta cttatgcttg gtccaactgc aaagctatta gctgaagatt tatcgcagct
     2761 aggacatcaa gctattgatt taggtcatat cgattcagaa tatgagtggt ttaagatggg
     2821 agcagtttct aaggtgaaac tttctcataa acatacggca gaacataatt ttgacgaaga
     2881 tattacttta atagaagaca atagttacga aaatcaaatt gtatcaaaaa ttggtacatc
     2941 agatagacag tacgctttga cagtgacgtt aacagatgat atatgggagc tagaacattt
     3001 attacaaaga tgtcctaata ctgattttca tattgctgcc ccagtttatt gttcggatag
     3061 acttaagcag ttagtaggtt atccaaatta ttacctacat gaagctatta ctgaagagca
     3121 attcgaagta ctcctattaa atagcgatat atatttagat attaatcatg gtgaagaagt
     3181 ttggaatgtt gttgatagat gtataactga gggtaaaact atatatggaa tcagacgggc
     3241 a
//
LOCUS       DATRSD010000132         2870 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRSD010000132 DATRSD010000000
VERSION     DATRSD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.133"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            14..84
                     /locus_tag="VBD85_001912"
     tRNA            14..84
                     /locus_tag="VBD85_001912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="VBD85_001913"
     tRNA            119..192
                     /locus_tag="VBD85_001913"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="VBD85_001914"
     tRNA            206..293
                     /locus_tag="VBD85_001914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
     gene            529..1830
                     /locus_tag="VBD85_001915"
     CDS             529..1830
                     /locus_tag="VBD85_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7134437.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQKQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNLTATSSATT
                     VAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSNTYPMGQCT
                     WGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGYGHVAVVTS
                     VANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1954..>2870
                     /locus_tag="VBD85_001916"
     CDS             1954..>2870
                     /locus_tag="VBD85_001916"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7134438.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIR"
BASE COUNT          883 a          487 c          613 g          887 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agagtaatta tactcttttt ttatttctct tttagtttca ctatggagta
      361 attatattga tagagtgaat agaagaaggt tatcgttttc tagatttgat tatggttcat
      421 ttagaaatag ttgatttcaa atataaatgt aacaatgacg taatataatt tcgtgaattt
      481 tttggtaaaa tatttaatgt cttatcgtaa aggagtagtt taataaaaat gaaaaaaaga
      541 atattatcag cagtccttgt gagtggagta actcttggaa ctgctgccgt aacagtaaac
      601 gctgatgact ttgactcgaa aattgctgct actgattcag taatcaatac tttatcaggg
      661 caacaagcag cagcacaaaa acaggtgact gctattaaag gacaagttgg tgctttagag
      721 tctcaacaat cagaattaga agcgcaaaat gctcaacttg aggcagtatc tcagcagtta
      781 ggtcaagaga ttcaaactct ttctaataaa attgttgcac gtaacgaatc tttgaaaaag
      841 caagttcgta gtgctcaaaa gggtaacttg actaactata ttaatactat tttaaattcg
      901 aaatcagtat ctgatgctgt taatcgtgtt gttgctattc gtgaggttgt ttcagctaat
      961 gaaaagatgt tagcacaaca agaggctgac aaagcagcct tagaagctaa acaaattgaa
     1021 aatcaaaatg ctataaatac tgtagcagct aacaagcaag ctatcgaaaa taataaagca
     1081 gccttagcaa cacaaagagc acaattagaa gcagctcaat tagagttatc tgctcaattg
     1141 actacagtcc aaaatgaaaa agcatcttta attcaggcaa aagcacaagc tgaggaagct
     1201 gcgcgtaagg cagctgaagc acaagctgag tctgtagcaa aagcacaggc agcagcacaa
     1261 gttgagtctg ccacagctcc tactgaaaca gttcaaacac aaccgagaac tgaaataaag
     1321 ccttctaatc ttacggctac ttcttcggca actactgttg ctaaaactac agctacagct
     1381 actaatgagc caaaagttac tcaaccttct gtagtaacta aagcagttga agcacctaaa
     1441 gcagttgttt cttcaacacc tagagcggta tcaaaacctg ttgtcagaag ttatgattca
     1501 agtaacacat acccaatggg acaatgtact tggggagcta agtcaatggc ttcttgggtt
     1561 ggtaactatt ggggaaatgc taatcagtgg ggtgctagtg cacgtgcagc tggttattct
     1621 gtaggaacta cccctcgtgt tggtgcagta gctgtttggc catatgatgg tggtggttac
     1681 gggcacgttg cagtagtaac gtcagtggct aacaattcat ctatccaagt tatggaatca
     1741 aactatgctg gaaatatgag cattggcaat taccgtggtt catttaaccc tagtgcttct
     1801 ggctctgttt actatattta cccaaattaa tgaatactaa ggagatcttt cgtggtctcc
     1861 tttttgttta ttaattaggt atggtttatt ttggattgaa aaattgtgta aaaagcgtta
     1921 aaatggtata ggactaaagt tttggaggaa atcatgtctt attctaattt aaaattgttt
     1981 gcactgtcat caaacaaaga actagcgaaa aaagtgtctc agacgattgg aattccgcta
     2041 ggtcaatcga cagttcgcca attttctgat ggtgagattc aagttaatat tgaagagtct
     2101 attcgtggcc atcatgtttt tattttacaa tcaacaagct caccagttaa tgataatttg
     2161 atggaaattt taattatggt tgacgcttta aagcgtgcta gtgcggagtc tgttagtgtg
     2221 gtaatgcctt actatgggta tgctcgtcaa gatcgcaagg cacgttctcg tgaacctatt
     2281 acttcgaaac tcgttgcgaa catgctagaa gttgctggtg ttgatcgtct tttaacagtt
     2341 gatttacatg cagctcaaat tcagggattc tttgatattc ccgttgatca ccttatgggg
     2401 gctcccctta ttgcagatta ctttgatcgt cagggattgg ttggtgatga tgttgttgtc
     2461 gttagtcctg atcatggtgg cgtaacacgt gcacgtaagc tggcacagtg tttaaagaca
     2521 ccgattgcta tcattgataa gcgccgtagt gttactaaaa tgaatacatc tgaagtaatg
     2581 aatattattg gtaatatcaa aggtaaaaaa tgtattttaa ttgatgatat gattgatact
     2641 gcaggtacta tttgccatgc tgcagacgct ttagctgaag caggggcaac agctgtttat
     2701 gcttcatgta ctcaccctgt tttatcaggg ccggctttgg ataatattca aaattcagct
     2761 attgaaaaat taattgtgtt agatacgatt tacctccctg aagaacgttt gattgataag
     2821 attgaacaaa tttcaattgc ggaattgatt ggtgaagcta ttattcgtat
//
LOCUS       DATRSD010000133         2823 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRSD010000133 DATRSD010000000
VERSION     DATRSD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.134"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..325
                     /locus_tag="VBD85_001917"
     CDS             <1..325
                     /locus_tag="VBD85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134439.1"
                     /translation="KKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQ
                     SGNGTPQGGDYNLSLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGI
                     WHIYE"
     gene            389..2023
                     /locus_tag="VBD85_001918"
     CDS             389..2023
                     /locus_tag="VBD85_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7134440.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAIMDVTGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLDQSEVDALVSDIKMSTVWNDGVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2042..2314
                     /locus_tag="VBD85_001919"
     CDS             2042..2314
                     /locus_tag="VBD85_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134441.1"
                     /translation="MSKELKLPLNEMLKEQERIAIKAIVATAKSTITDTGTNLKATLE
                     SDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2324..2674
                     /locus_tag="VBD85_001920"
     CDS             2324..2674
                     /locus_tag="VBD85_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134442.1"
                     /translation="MIDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIDLRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1068 a          477 c          518 g          760 t
ORIGIN      
        1 taaaaaaatg acaaaaaaag aacaattagc ctatctcaaa gagcatgagc aagaaatcat
       61 agattatgta aaattacata acaaccaaat tgagtccgtt caattcgatt ggtcaagtgt
      121 aaaagtagaa caaagcggga atggaactcc acaagggggt gattataatc tttcactgag
      181 aggaaagttt aatcatctac aaaattcaaa attaatagtt gatttttatt tagctcataa
      241 aaatgatatc ccaaatatca aatcaatggg aatgctaaat aagccgtata tacataaaaa
      301 tggtatttgg cacatttatg aatagcttga atcatcctat ataaagtaat agaattttta
      361 ccacaagcaa ggtagaggag aagaataatt gagtaattgg aacttagata attttgataa
      421 cttacatagc tctttagcag aaagtgcata taatagtcga cctaattcat ttcctgaatt
      481 atttgagact gactcagtaa cagaagtaaa attttctcaa ccctcagaag ataacaaagg
      541 tcaaatcaca cagggtggca ctaacctccc caatgatggt attgtctacc tgcaaccaga
      601 taagtcctta aaatctattg atgaaaatgt aaaggtcctc attccagatg tcaatggagg
      661 ctatcacact gagcattatg ttactcattc ttatcaaaaa ggtgtgctta ctgacgataa
      721 ggcaggtttt aatgcttact acctgtcaga tactgaaaaa atagatagca ctacaaagca
      781 cacttatcta gccattcgtg gtagtgatgg gattggtcta gatacactaa acgattgggt
      841 tagtaacaat gcaatgtttg ctgtctctaa caagtatatt ccacaagcga aattggctaa
      901 taaggctatg aaagaaaaaa ttgccgagct aaaaggaaaa gcgcctggag ctattatgga
      961 tgttactggt cactcactcg gtacgattgt atcttctcaa gcagttgtta atcttagcta
     1021 tgcagaattg gagaatgttg gtcaagtagt ccttttcgat ggccctgatg tatcaagaag
     1081 cttggaaaaa atggaaggca tttctgctaa aaaaatccaa gaagctggga agcatgttac
     1141 ctactatgtc aatccctttg atatagttag tatgcttaat cgtgaaaagc catggaaaga
     1201 ccaattcggg aaagtaaatg tcattgttcc tagtaaatat acgggtactt ttgatgagca
     1261 atcttctcac gattttggtg ccttccaaat tgatagtagc ggaaatcctt tagttgcttc
     1321 tgaaagctat catccagaat tattaacatc tggtcaagac ttagctagac tcgaaaaaaa
     1381 attcataaag aatcttttag gagagcatta tgattcggat aagctaggac cagtactcaa
     1441 aggcgctctg actggggacc tgactcgact aattgcaaca ttaaaagctt gtggtatcag
     1501 tgtcactgta aacgaagcta aaaaactgta cgataacttc caaaaagact acaaagccat
     1561 aatttccaaa gctaaaaaag atggcctcga gtgggatcgc aaaaacatcg caagcttcca
     1621 tagtaggatc aaatcagcat caggcagtca acggatttta cttagaactc aaatgctcta
     1681 tatggcaacg caattagcag aagcagatgt ttcagaaaaa gtctctgcaa caaaaaaaca
     1741 tattagtgaa gctaaggata cgattgtaga aatatcgaca tccactattt catcagctga
     1801 aataatggct atgcaccttg accaatcaga agttgatgct ctggtttcag atataaaaat
     1861 gtcaacagtt tggaatgatg gcgtagagac ttctgattat gaagcacttg atcactataa
     1921 aacaaaaatg accacattta cgaccaattt ggtaacagtt gcacaaaatt taacagctca
     1981 agatgagcaa ctagctggtg atattgtcac gaacttatcc taaaatagag agattaaatg
     2041 aatgtccaaa gaattgaaat tgcccttgaa tgaaatgttg aaagaacaag aaagaatagc
     2101 tattaaggca atagttgcta cagcaaaaag cactattacg gatactggaa caaatttaaa
     2161 agcaacttta gagtcagatg cccaagacct aaaaaactat gcatccacct ataacgccaa
     2221 tatcacggat ggtcttgaag gccagtcagc tcaagcagca tctgatttct tgacacaact
     2281 tccaaaacca gagttagata atccagtcaa ttaggaggct agtatgatag atacaaaaaa
     2341 actgcaagaa ctcgatcaag aatatgatca aaatctcaga aacatttatc gtaacagaga
     2401 gcaactagaa gatgactttc acttgtttat ggcaagaaca gacagcctaa aagagtctgt
     2461 ctatcaagca actttaggac aaggttggga attaccccaa gaagctcatg cacacctata
     2521 taatatggat gataataagg atacattcat atcagaattc aatgaatata tggaaaagct
     2581 tgaagaaaag gaaatagatc ttcgaagagt ttataatgat agagtagatg aattgtatca
     2641 gaaggctaag caaaacgaag caaaaaaagg ataaaaattt tttcaagtaa tatatataaa
     2701 tatgaaaaat aaagaatctt gcagtaaaat gagtatttat ttttcacgac gaacagtcac
     2761 tacaaaatca ctaaaatctc aaaattactt ggtttttaag ttaatgtcta atactcaaac
     2821 ctt
//
LOCUS       DATRSD010000134         2818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRSD010000134 DATRSD010000000
VERSION     DATRSD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.135"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(18..815)
                     /locus_tag="VBD85_001921"
     CDS             complement(18..815)
                     /locus_tag="VBD85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7134443.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMANAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            1001..1861
                     /locus_tag="VBD85_001922"
     CDS             1001..1861
                     /locus_tag="VBD85_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7134444.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGFDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQEDRPGIFLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            1904..2635
                     /gene="yaaA"
                     /locus_tag="VBD85_001923"
     CDS             1904..2635
                     /gene="yaaA"
                     /locus_tag="VBD85_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO7134445.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSISEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFETVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTAMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
BASE COUNT          957 a          524 c          410 g          927 t
ORIGIN      
        1 atttagattt gctatcctta attttccata tactctacta aagcataata aataccatcg
       61 ttatcattat cctgagttac atttccaatt ctctcttgta aatctttagg agcattcgcc
      121 atgacaaaac ctttacccac aatttcaagc agatttaaat cattatagtt atcaccaaac
      181 gcaatagagt ctgcaagaga aatatctaaa tagtctgcta aagttttagc agattttcct
      241 ttttcaatac ctggagccat tatttcaata aagtaaggag cagattgtgc gattgataaa
      301 tgtggatatt gagctttcaa agtgttctcc aattctccca tcagagatgg ttctcccatc
      361 aagagaagtt tatgaatcct atcttgcttc tctaactcca tcaaactcat ctctttagat
      421 tgtaaaccaa caatctcctc ctctttttgg acccactcat tttctttatc acaggaatac
      481 cattcatgat aacgatagac attccatgca attttcgaat aatgcttgct tacataagaa
      541 caaacttgta aggcaacttg accatccatt gtgtgggaag caattgtttt ttgttgcata
      601 tcttgtatta aagccccatt ataggaaata atataagaat ctattccaat ttgttccata
      661 atcggagtaa ttgcctcagg catcctagct gatatgggaa cgaaagtagc cccttgagct
      721 actatttgtt taattccctg ccgtgtctta ggtgttactt taaaattact gttaattaaa
      781 gtaccatcaa tatcactaaa tattagcctt accatattat cctccaaaat ataattgtac
      841 gcgcttactt tagtctaaca cttaaatttt tgtttcgcaa ataaacttcg tctggtctaa
      901 tgagactact aatataagct atatttcaaa acaaattagt ttatttctct caatgaacgg
      961 tgtaatatgt tactatatag taataacagg aggattaact atgccgtatc catacaaagc
     1021 caatcattcc attgaatcaa tcaccttaaa agtaaatgac ctcgaaaacc tcgtaaactt
     1081 ctatagcgat atcattgggt taactgttat tgacaaatcc agcactagag ccttactagg
     1141 ggtaaatcaa aaaatccctc ttattatcct tgaaaaaact gaacttgaaa agcatagtac
     1201 ctatggcctt tatcacactg ctattttagt accagatgaa taccatctca gtctagctct
     1261 aaatcattta ttatctcagc atatccctct agagggtgga gctgaccatg gctatagcaa
     1321 tgctatctat ctaagtgatc ctgaaggtaa tggaatcgaa atttataatg ataaggatat
     1381 ctctatgtgg gatatcagag agagtggaca aattattggt attactgaaa gactagatat
     1441 tgacaattta cttgacagcc ttgtcaacgt tcctaataat tataagcttt ctgaaaaaac
     1501 tcgtattggt catattcatt tgagtgttaa agatgctaag attagctcaa aactctatca
     1561 aaatgttttt ggctttgatg agaaatttgc aatcccgaca gctagttgga ttgcatccgg
     1621 caactaccat catcatctag cctttaataa ttgggctggt ccaaatcttt ctaaaaacca
     1681 agaagataga cccggaattt tcttattgac aattgcttac aatgatgaca acctttttag
     1741 agatagctta aaaaaagcgc aactttacca actgactttc cttgaaaaac aagatcatta
     1801 ttatattatt gaagattttg atggcatccg aattaaagtt gttctaaaac cagagaatta
     1861 accattactt gacttttgcc tcttcatttt acataatgtc aatatgataa aaattttgat
     1921 cccaactgca aaagaaatga aagtgtgtca aaacattgca aggcctaaac tgagtgcaca
     1981 aacgcaaatt ataattgatt atttttctac tttaactgtt tcagacttag aggatatcta
     2041 cagaattaat acaagtgcag ctcgttgtga ggctcaaaga tggcaagatt ttaaatgtaa
     2101 acaacttact ctgaatcctg ctattaagtt attcaacggt cttatgtacc gcaatatcaa
     2161 aaggcacaac ttatccataa gtgaagcaca atttatggaa aattctgttt ttattactag
     2221 tgctctttat ggcatcattc ctgctatgac actaatatcc cctcatcgtt tagattttaa
     2281 cactaaaatc aaaatcaaca ataactctct taaagttttt tggcgtgaaa attacgatac
     2341 ttttatgcag tcagatgaca ttatggtttc tttgctttct aatgaatttg aaaccgtctt
     2401 ttctcctaaa gagcgtcaaa aactaataca tcttaacttt attgaagatc gagatggcca
     2461 attaaaaacc cactctacca tatcaaaaaa agcaagagga aagtgtttaa ctgctatgat
     2521 ggaaaataat tgccaaactc tagaacactt aaagcaatta cgctttgatg gcttctgtta
     2581 tgataatgaa ctctcagact ctaagcaatt aacttttgtt aaaaaacaga cttaagtaac
     2641 tatttatcca caatttattc acacaaaaag caagagataa tcgtatctct tgctttttga
     2701 caaacttgta aggtctaaaa acctctctat atcaaggttt caaataatat taacaaaaaa
     2761 taattgttat caacaacttg tggataacta tggggataga cagaggataa tctgttta
//
LOCUS       DATRSD010000135         2812 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRSD010000135 DATRSD010000000
VERSION     DATRSD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.136"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(2..529)
                     /locus_tag="VBD85_001924"
     CDS             complement(2..529)
                     /locus_tag="VBD85_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO7134446.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGQEFGVENTKRLT
                     LAHIIFYLSCFVEAMVHKTNFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKFVDHFLFRTVKHPNYFLNILPELVGLALVSHAYVTFIFIFPVYAVILYQRIAEEE
                     KLLQEVIIPNGRMKG"
     gene            complement(638..1978)
                     /gene="bgrS"
                     /locus_tag="VBD85_001925"
     CDS             complement(638..1978)
                     /gene="bgrS"
                     /locus_tag="VBD85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase BgrS"
                     /protein_id="HEO7134447.1"
                     /translation="MIKNPKLLTKSFLRSFSLLGAIALVIHIAIYLIFPFYYIQQEGE
                     KFNESATIMSRYLEGKNFHELPSLLESYSKSLRISAHLKDDVLDKRISLVHDLEIKEG
                     SPTNYIVTIDRQITTADGKKVTIQFIQGVDIYKEATRIMLLYLPYTFLATLVFAFLFS
                     YFYTKRLLEPLFYISKVTGKMQELDHDIRFDETRKDEVGEVGKQINDVYENLLTVIDE
                     SERRNERIVRLQKQKVSFVRGASHELKTPLASLRIILENMQYNIGDYKDHPKYIAKSI
                     DQIDQMSHLLEEVLESSKFQEWTECSETLIVQTILTDVLSRYQELARSRGIEIENQLT
                     DATRVVMSLKALDKVLTNLISNAIKYSNKNGRVILSEQDGYLSIKNTCQPLNQEELNH
                     LFDIFYHSQVVTDKSEGSGLGLYIVNNILESYQMSYSFLPYEQGMVFKICLQAD"
     gene            complement(1975..2628)
                     /gene="bgrR"
                     /locus_tag="VBD85_001926"
     CDS             complement(1975..2628)
                     /gene="bgrR"
                     /locus_tag="VBD85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor BgrR"
                     /protein_id="HEO7134448.1"
                     /translation="MNILVVDDEDMIREGVAAFLREEGYHVILAKDGQEALEKFHEFP
                     IHLMVLDLMLPKRSGFEVLKEINGNHDIPVIVLSALGDEETQMQVFDLYADDHVTKPF
                     SLVLLVKRIQALLRRYYVVEDIWHYQDVAVDFTSYQARVKNEEVAIKPKELLVLKCLI
                     QHKNQVLSREQILESISNDFADLPLDRVVDVYIRNLRKKLDLDCIVTVKNAGYKISL"
BASE COUNT          916 a          600 c          429 g          867 t
ORIGIN      
        1 tttaaccttt cattcttcca ttcggaataa taacttcctg caataatttt tcttcctctg
       61 ctattctttg ataaagaata accgcataga caggaaaaat aaagatgaat gtcacataag
      121 catgactcac cagggccaaa ccaactagtt ctggtaatat atttaaaaag taattaggat
      181 gctttacagt cctaaaaagg aagtgatcta caaatttgtg attattgaca agcataagct
      241 ttactgtcca aatatctccc aacaagtgaa tcaccaacat caacatcagc atagaaaaaa
      301 taagcaagac taaaccaacc atgctcatgc catcaaaatt tgtcttgtgc accattgctt
      361 caacgaagca agagagataa aaaataatat gcgccaacgt caatcgtttt gtattctcaa
      421 ccccaaattc ttgcccaccg ttgctaagga ttcgtttctc attttctatc gaacgcttta
      481 aaaaaatcaa tcgaataagg aatgttacta aaataattgc taataccata ttagtatcct
      541 ttcttttatc tatacaaaga ataaccccat atgttaaccc tatatgaact ataacaaaaa
      601 aagaacggag agatttccgt tcttttactt tatacaacta atctgcttgc aagcaaattt
      661 taaataccat tccttgttca taagggagaa aactatagga catttgataa ctttctagga
      721 tgttattcac gatatagagt cccaaaccag acccctcaga cttgtctgtg acaacttgag
      781 agtggtaaaa aatatcaaac aaatgattca actcttcttg attaagaggt tggcaggtat
      841 ttttgataga aagatagcca tcttgctcgg ataggattac gcgcccattt ttatttgaat
      901 atttaatcgc attactaatc aggtttgtca aaaccttatc caatgccttg agactcatga
      961 cgaccctagt agcatctgtc aattgatttt caatttctat tcctcttgag cgtgctaatt
     1021 cctgatagcg agataaaaca tccgtcaaaa tagtctggac tatcaaggtc tcgctacact
     1081 ctgtccactc ttgaaactta gaggactcca aaacttcttc gagcaggtgg ctcatttggt
     1141 caatttgatc aatactcttt gctatatact tagggtggtc tttataatcc ccaatattgt
     1201 actgcatatt ctctagaata attcgaagac tggctaaagg ggttttcaac tcatgtgacg
     1261 ctccacgaac aaaagaaact ttctgctttt gcaacctgac aatccgttca tttcttctct
     1321 cagattcatc aatgactgtc agcaagtttt catacacatc attgatttgc tttccaactt
     1381 ccccaacttc atcctttcgc gtttcatcaa agcgaatatc atgatccaac tcctgcatct
     1441 tacctgtaac ttttgagata taaaagagag gctccaataa acgtttcgta tagaagtagg
     1501 aaaagagaaa ggcaaataca agcgttgcta aaaaggtata cgggagatag agaagcataa
     1561 tccgtgttgc ctctttataa atatccacac cttgaataaa ttgaatggtt acctttttac
     1621 catcagctgt cgtaatctga cggtcaatcg ttacgatata gttagtggga ctgccttcct
     1681 ttatttccaa gtcgtgcaca agggaaattc tcttatccag tacgtcatct ttcaaatggg
     1741 cagagatgcg aagagatttc gaataggact ccagcaaact aggcagttca tgaaaatttt
     1801 tgccttctaa gtacctgctc ataatagtcg cactctcatt aaacttctca ccctcttgct
     1861 gaatgtaata aaaaggaaag atcagataga tggcaatgtg gatgaccaaa gcaattgcac
     1921 ctaaaagcga aaaacttctt aaaaatgatt tggttaataa tttaggattt tttatcataa
     1981 gctaatttta tatccggcat ttttcactgt tacaatgcaa tccagatcca acttcttacg
     2041 aagatttcga atgtaaacat caactactct atcaagaggc aaatctgcaa aatcatttga
     2101 gatagactcg agtatctgct ctctactcaa gacctggttc ttatgttgga tcaaacattt
     2161 taaaaccaac aattctttcg gcttaattgc aacctcctcg ttcttgactc gagcttggta
     2221 ggatgtgaaa tccactgcca catcttgata atgccaaata tcctccacaa catagtaacg
     2281 cctaagcagg gcttgaatac gcttgactag gagcaccagt gaaaatggtt ttgtcacatg
     2341 gtcatcagca taaaggtcaa atacctgcat ctgagtttcc tcatctccga gcgcacttaa
     2401 gacgattaca ggaatatcat gatttccatt aatttctttt aacacctcaa atccactccg
     2461 cttcggtaac atcaaatcca agaccatcag atgaataggg aactcatgaa atttctccaa
     2521 agcctcctgc ccatcctttg ctagaataac atgatagcct tcttctctca aaaaagcagc
     2581 tactccctct ctaatcatat cctcatcatc tacaactaaa atgttcatct atttcccttc
     2641 ttagactgaa acaaaacttc ctatgtttta gtcaatgtta ttgttatttg atatgcaaaa
     2701 aagaaacagg accatattga aacgatgaaa gtttcagaaa cactgttctt tgtcatttta
     2761 tacaagtctc actattaaga tctacctatc attattatag ctactattca ga
//
LOCUS       DATRSD010000136         2753 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRSD010000136 DATRSD010000000
VERSION     DATRSD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.137"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            139..807
                     /locus_tag="VBD85_001927"
     CDS             139..807
                     /locus_tag="VBD85_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7134449.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            804..1742
                     /locus_tag="VBD85_001928"
     CDS             804..1742
                     /locus_tag="VBD85_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7134450.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(1785..>2753)
                     /gene="xerS"
                     /locus_tag="VBD85_001929"
     CDS             complement(1785..>2753)
                     /gene="xerS"
                     /locus_tag="VBD85_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO7134451.1"
                     /translation="FTTLYEYLKEYRRFLEWLLDSGVANCHHIAEIELSVLENLTKKD
                     MEAFILYLRERPLLNANTRQNGVSQTTINRTLSALSSLFKYLTEEVENADGEPYFYRN
                     VMKKVSTKKKKETLASRAENIKQKLFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNK
                     ERDLAIIALLLASGVRLSEAVNLDLKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLA
                     NYLDIRKNRYKAENQDIALFLSEYRGVPNRIDASSVEKMVAKYSQDFKVRVTPHKLRH
                     TLATRLYDATKSQVLVSHQLGHASTQVTDLYTHIVNDEQKNALDKL"
BASE COUNT          909 a          430 c          478 g          936 t
ORIGIN      
        1 gaacttatta tcatattatt gaacgctaaa aaggattggt tattttacca atccttttgc
       61 attaatagaa accgatagac taaaaaattt gttatactaa atgaaagaaa gagatttcgt
      121 actattttag gaggggatat gtcacaagag caaggaaaaa tttatattgt agaagatgat
      181 atgacgattg tgtcactttt aaaagatcat ttatcagcta gctatcatgt ctctagtgtc
      241 agcaattttc gtgatgtgaa acaagaaatt atcgcatttc aacccgattt gatactaatg
      301 gatattacgt taccctattt taatggtttt tactggactg cagaattgcg taagttttta
      361 acaattccta ttattttcat ttcatctagt aatgatgaaa tggatatggt tatggcatta
      421 aatatggggg gtgatgactt tatttcaaaa ccattctctc tagctgtatt agatgctaag
      481 ctaactgcta ttttaaggag aagtcaacaa tttatccaac aggaattaac ttttggggga
      541 tttacgttga caagagaagg gttattgtct agccaagata aagaggttat tttatcgcca
      601 acagaaaata aaatcctatc tatcttgctc atgcatccta aacaagtagt ctcaaaagag
      661 tctctattag agaaactttg ggaaaatgat agttttattg atcaaaatac acttaatgtt
      721 aatatgacac gcttacgtaa aaaaattgtc ccaataggtt ttgattacat tcatacagtg
      781 agaggagttg ggtatttact acaatgatac gccagttttt aagagaacac ttgatttggt
      841 atattttata tatcatgatg tttgtcctat tttttattag tttctatcta tatcatttac
      901 caatgcccta tttgtttaat tccttaggtt taaatgttat tgttttacta ggaattagta
      961 tttggcaata cagtcgttac aggaaaaaaa tgttacatct caaatatttt aatagtagtc
     1021 aggacccctc tttcgaactt caaccgagtg attacgctta ttttaatatt attacacaat
     1081 tagaagctag agaagcgcaa aaagtttctg aaacaattga acaaaccaat catgttgcac
     1141 ttatgataaa gatgtggtcg caccaaatga aagttccatt ggcagctatt tcattaatgg
     1201 cccagacaaa tcatctcgat cctaaggaag ttgaacaaca attattgaaa ttgcaacatt
     1261 atcttgaaac gttgttagca tttttgaaat ttagacaata tcgtgacgat tttcgttttg
     1321 aagctgttag ccttagagaa gtagtagtag aaattataaa atcgtataag gttatttgtc
     1381 tatccaaaag cttatctatc ataattgaag gcgataatat ctggaaaaca gacaaaaagt
     1441 ggttaacttt tgctctttca caggtgctag ataatgccat aaaatattct aatcctgagt
     1501 caaagataat aataagcata ggagaagaga gtattagaat acaagactac ggtatcggca
     1561 tactcgaaga agatatccct agactttttg aagatggctt tacgggttac aacggtcatg
     1621 agcaccaaaa ggcaacaggc atggggttat atatgacaaa agaagtctta tctagtctga
     1681 atttgtccat ttcggtggat agcaaaatta attatgggac tgctgtttct atacataaat
     1741 aaagttatgt aaaataaaat atttacataa ctttatttat gttactatag tttatctaaa
     1801 gcgttctttt gttcatcgtt aacaatatgg gtatataggt cagtaacttg agtggaagca
     1861 tgacctagtt ggtggctaac taatacttga gattttgtag catcatatag cctagtagca
     1921 agagtatgcc ttagtttatg aggtgttaca cgaaccttaa aatcctggga atacttagca
     1981 accattttct ctacacttga agcatcaata cgatttggca ccccacggta ttctgataaa
     2041 aatagagcaa tgtcctgatt ttctgctttg tatctatttt tacggatgtc taaataatta
     2101 gcaaggtagg gcttcgcaaa gctagcgaca ttgacagagt cccttttccc cccttttcgt
     2161 gtgacatcaa taaccattac attaaggtta atatccttta aatctaagtt tactgcctca
     2221 gatagacgca ctcctgaagc tagtagcaaa gcaataatag ctaaatctct ctctttattt
     2281 ttgttgaaaa aagctaaagc acgtttggaa agtttgtttt gatattcaca atctatatac
     2341 tctaagaatt ctatcgtttc attgcccaaa aataactttt gtttaatatt ttctgcccga
     2401 gaagctaaag tttccttttt ctttttggtt gagacttttt tcatgacatt gcgataaaag
     2461 taaggctccc cgtcagcatt ctcaacctct tcagtaaggt atttgaaaag actagataag
     2521 gcagatagag ttctgttaat ggtggtttga gacaccccat tttgccttgt gttagcattt
     2581 aataaagggc gttctcgtaa atatagtata aaagcttcca tatctttttt agtaaggttt
     2641 tctaaaactg aaagctcaat ttctgcaatg tgatgacaat tagcaacacc agaatccaat
     2701 aaccattcaa gaaaacgtct atattctttt aaatattcgt agagtgttgt gaa
//
LOCUS       DATRSD010000137         2604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRSD010000137 DATRSD010000000
VERSION     DATRSD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.138"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            326..685
                     /locus_tag="VBD85_001930"
     CDS             326..685
                     /locus_tag="VBD85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134452.1"
                     /translation="MVIRLRLELEEEFLILSDIYMNNGYEKYIQFVQGNVYFINLELD
                     KTPNYANLKLILESIFDHNNQKIFIPKLYEEYIYILISLGEVEKAKEILREVSDYLTE
                     ESLNYFEKEYDKAKGSN"
     gene            707..2005
                     /gene="purB"
                     /locus_tag="VBD85_001931"
     CDS             707..2005
                     /gene="purB"
                     /locus_tag="VBD85_001931"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO7134453.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            2158..>2604
                     /locus_tag="VBD85_001932"
     CDS             2158..>2604
                     /locus_tag="VBD85_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7134454.1"
                     /translation="MLKHFGSKVRKFRESQQVTREEFCGDESELSVRQLARIEAGESV
                     PNLSKAHYIARNLGVSLDSLTAGASLELSKKYKELKYLILRTPTYNDTDRLDLIEKYL
                     DEIYLNFYDDLPEDEKIAIESIQSSLDIYISKSHHFGDDLIREYMNQ"
BASE COUNT          879 a          390 c          539 g          796 t
ORIGIN      
        1 atggttatac caaaataaat ctgttacaga acccttccta ttaaaaaagt tagctatcta
       61 ttttattttt ttcattgcca ctttaattgg taattttaaa aatgagtcgt caatcttaga
      121 aacaccatta ctttttatca gtatattttt tactatggat agaattattg ctctatctaa
      181 agaaatgcgt gatttgatta tatctaagag tattttattt tattatgatc atgaaaaaat
      241 aaagccagca atattactta gtgagatgaa agaaattaag tatcttgaaa atgtagatat
      301 tggagaactt gaacttgtta ggcaaatggt aattaggttg cgtctagagt tagaggaaga
      361 atttttaatt ttatcagata tatatatgaa taatggctat gaaaaatata tacaatttgt
      421 tcaagggaat gtttatttta tcaacttaga attggataaa actcccaatt acgctaactt
      481 gaaattaata ctagaatcaa tatttgatca taataatcaa aagatattta tacctaaact
      541 ttatgaggaa tatatatata tcttaatttc tttaggagaa gttgaaaaag ctaaagaaat
      601 attaagagaa gtgtcggatt atttaactga agaatctttg aattattttg aaaaagaata
      661 tgataaagct aagggaagta attagaattt ttaaaggaga caaacaatga tcgaacgtta
      721 ttcacgccct gagatggcgg caatttggac agaggaaaat aaataccgtg cttggttgga
      781 ggtcgagatt ttggctgacg aggcatgggc tgagttgggt gagattccta aggaggatgt
      841 ggctaagatt cgtgagaagg cggattttga cattgaccgc attcttgaga ttgagcagga
      901 tacgcgtcac gatgtggtgg ctttcactcg tgcggtttct gagacgcttg gtgaggagcg
      961 caagtgggtg cactacggtt tgacatcgac tgacgtggta gacactgcct acggttacct
     1021 ctacaagcaa gctaacgaca ttattcgtcg cgatcttgag aatttcacaa atatcgtggc
     1081 tgataaggct cgagaacata aaatgaccat catgatgggt cgtacccacg gtgttcacgc
     1141 tgagccaacg actttcggtc ttaagttggc aacttggtac agtgagatga aacgtaatat
     1201 tgagcgtttt gaacatgctg ccgcaggagt ggaagctggt aagatttcag gtgctgttgg
     1261 taactttgcc aacatcccac cttttgtgga agaatatgtc tgtgacaaat taggcatccg
     1321 tccgcaagaa atttcaacac aggttcttcc acgtgatctc cacgcagaat attttgcagt
     1381 gcttgcaagt attgcaacat ctatcgaacg tatggcgaca gagattcgtg gtctgcaaaa
     1441 atcagaacaa cgtgaagttg aagagttctt tgccaaaggt cagaaaggta gctctgctat
     1501 gcctcacaaa cgcaacccga tcggttcaga gaacatgact gggctagcgc gcgtgattcg
     1561 tggtcacatg gtgacagctt atgagaacgt ggcactttgg cacgagcgtg acatttcgca
     1621 ctcatcagct gagcgtatca tcacaccaga cacaactatc ttgattgatt acatgctcaa
     1681 ccgttttggc aatattgtta agaacttgac tgtcttcccg gaaaatatga tgcgcaatat
     1741 ggaatcaact tttggtttga tttatagtca acgtgttatg ctcaaattga ttgaaaaagg
     1801 aatgacacga gaagaagctt atgacttagt tcaacctaag acagcttatt cctgggacaa
     1861 tcaagtggat ttcaaaccac ttttagaaga agacaccaaa gttacctctt gtcttacaca
     1921 ggaagaaatt gatgaactat ttaatccgat ttattacaca aaacgtgttg atgatatttt
     1981 tgaaagacta ggattagaaa aataagaaaa ttccattacc ggttaacgtt tttttaacca
     2041 gtaatggaat ttttattgat ttaaaaggaa ttaatattaa aaaaacatac ttaaaatgag
     2101 tttattatga taatatataa taagaagaaa aatttaggag ttagttttta aaggaaaatg
     2161 ttaaaacatt ttggtagtaa agtaaggaag tttagggaat ctcaacaggt gacacgagaa
     2221 gaattttgtg gcgatgaatc agagttatcg gtccgacagc tggctcggat tgaagcagga
     2281 gaatctgttc caaatttatc taaggcgcat tatatcgcta gaaatctagg agttagttta
     2341 gattccttaa cagcaggagc gagtttagaa ctgtcaaaaa aatataagga attaaagtat
     2401 cttatattaa ggacgcctac ctacaatgat acagatagac ttgatttgat tgaaaaatat
     2461 ctagatgaga tttatttaaa tttctatgat gatttacctg aagatgaaaa aatagcaatt
     2521 gagtccatac aaagttcttt agatatttat atttctaaaa gccatcattt tggagatgac
     2581 ctgattaggg aatacatgaa tcaa
//
LOCUS       DATRSD010000138         2593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRSD010000138 DATRSD010000000
VERSION     DATRSD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.139"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(72..1277)
                     /locus_tag="VBD85_001933"
     CDS             complement(72..1277)
                     /locus_tag="VBD85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO7134455.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            complement(1702..1890)
                     /locus_tag="VBD85_001934"
     CDS             complement(1702..1890)
                     /locus_tag="VBD85_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7134456.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
     gene            complement(1932..2132)
                     /locus_tag="VBD85_001935"
     CDS             complement(1932..2132)
                     /locus_tag="VBD85_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7134457.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            2310..2593
                     /locus_tag="VBD85_001936"
                     /pseudo
     CDS             2310..2593
                     /locus_tag="VBD85_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          919 a          487 c          357 g          830 t
ORIGIN      
        1 acttaattaa ctcactcctc taaaatattt cgattattaa aaggctggat tatctttcca
       61 accttttttt gttatatact acgatacttc aaatgtgatc cattagcctt ggcctgacga
      121 actgtatttt gataagacat ggataagacc aatccaattc caataagatt acttagtaat
      181 gaacttccac cttgcgaaat aaacggaagt gggattcctg ttagaggtaa tataccaact
      241 gctgcaccaa tattttcaaa aacatggaaa acaatcatca tgataaaacc agttgatata
      301 aaagtataaa attgattatt agattcaatg gtgatcctta acatacggta aatgataatg
      361 agatataacc ctaaaacaat cgccgaacca atgaagccaa aattctcagc aatgacagta
      421 aaaatcatgt cactttcacg aacaggaacc gagagctcta aaatattaaa acctttacct
      481 gttaccccgc cacttccaat tgataccata ccctgtgttt gttgataagc aattgagtct
      541 gcaaaactaa atggatcaat ccaagcagat aaacggttga tttgatatgt atccatcccc
      601 aaattataaa accattcctt gccgtttggt gagataaaaa tcatcaaaaa gctagctata
      661 aaaagaacga tagtagataa aattggcaag attatccacc aagatatgcc cgataaaagg
      721 attaaacctg acaaaatggc aaggaaaact aatgctgttc ctaaatcttt ttgtaacatt
      781 agaagaatca tcactggcaa tgtaaccaaa ccaaacaatc ctagcaactt ccaatcatct
      841 tgaaaagttt tgcgattttt ctgatgaaaa ctaacagtta ttcgagataa cattaaaata
      901 taggaaatct tcataaactc tgacggttga aacaaagtta ccgagccaat ggtcacccaa
      961 tttttagcac ctgttgccgc aaaaagctgt ggactataaa aaattaaggg aagaaccatt
     1021 agggttagtc ctaaagcata cagatagggc gtggctttcc ataaaaattc agtactgaaa
     1081 agcataacga caaatgcaat aatacacccc ataataatcc aagacacctg ctgaaacatc
     1141 gcgatataga tattagaagg ataatcattc atcgttgcaa cataaattga ggctaaacca
     1201 atcattaata aaaaaaatac tggtatcaca acagcataat caatttgccc tttaaaatag
     1261 tttgattttt gtgacatagg acttcctttt ttattcatgg taattattat aacatttttt
     1321 cagaatctga cttatatttt ttgcaataaa aaatgcaact caaatcatta tgagttgcaa
     1381 atattaggag attgttcttc atcagtaaaa caaagaacat gaaaagaaaa gtttatttag
     1441 gatatttaaa gtatacaaag ttcaccttaa acaaaactta aataagatta tttaagtttt
     1501 gttattttct caacactagt ctaaagaata aaatccttcc taacatctca aaaaaggttt
     1561 taatatgtcc cctgccaact tctctaccat taaaatagga tatattaaag gttaaaatac
     1621 acgatacatc cataataaaa agcatatttt tttattttat tctattttaa aatacttttt
     1681 ggggcgaatt ttggtccgtt attatgataa cttcctcagt acatcaagca ctctctcaac
     1741 tgtaacaacc tcttcttctc tcacttcttc gtgtagtaac acataatcaa atatctttcc
     1801 gtttttacgc actatcgcta ctgtgttccc tgaaatatag cctttatcaa tcgcttcttt
     1861 aaactcatct atatctgttc tgatagacaa aaagataaca aataaccgct cgttaaatga
     1921 gcggttattt gttacttcag tttttctttg acagcatcta ctgccccttc aacagcatct
     1981 ttagcatcat ctgctagttc tttgccttta gcaattgttt tttcgataaa tccttttgct
     2041 tctaactctt tatcaccggt tattttgcca aagccttctt tagcaccacc ttttgcttga
     2101 tctaattttg attttagttt ttcttgtgac atactgcacc tccttattat ttttattaat
     2161 cataacatct atatctttct ttagcaaata aaaacaagag tcgctagtgt caggcgactc
     2221 ttgttttagt gattatctca tggttatgcg agtatggatg tatactataa aaatagacaa
     2281 cactttgtgt gttataacct gttttaaaag acacaaaaat ctctgaagga agaatttacc
     2341 gtctgatgaa gaatatggcg ctccctaaaa tggctaccgt taagacaaaa acagctctta
     2401 aaaagactca aaaaacgtat cctcaaaact tactcaacca gaaatttaat cctgataaac
     2461 ctaatcaagt atggtctact gacttcacct atatttctat tggatataag aaatatgtct
     2521 atctctgcgc aataattgat ctctattcta gaaaatatat tgcttggaaa ttgagtcatc
     2581 gtatggatgc aaa
//
LOCUS       DATRSD010000139         2571 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRSD010000139 DATRSD010000000
VERSION     DATRSD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.140"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..121
                     /locus_tag="VBD85_001937"
     CDS             <1..121
                     /locus_tag="VBD85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEO7134458.1"
                     /translation="CHKIGKSEHYTYSQKFVDFIISEIEKDPQHFVESLKKSK"
     gene            460..1626
                     /locus_tag="VBD85_001938"
     CDS             460..1626
                     /locus_tag="VBD85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7134459.1"
                     /translation="MKITEVKKKDGTVIYRASIYLGTDKVTGKKVTTKITGRTKKEVR
                     EKAKQEAIEFIKNGSTRFKATSITSYQELATLWWDSYKHTVKYNTQLATEKLLTVHVI
                     PIFGAYKLDKLTTPLIQSIINKLADKTNKGERKAYLHYDRIHALNKRILQYGVIMQAI
                     PFNPAREVILPRNTKKANTKRVKHFENDELRTFFNYLNNLDKNKYRYFYEVTLYKFLL
                     ATGCRINEALALNWSDIDLDNAVVHITKTLNYKQEINSPKSKSSYRDIDIDSRTVTML
                     KQYRRRQIQEAWKLGRSETVVFSDFIHKYPNNRTLQTRLRTHFKRANVSNIGFHGFRH
                     THASLLLNTGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFFETAINSI"
     gene            complement(1733..2344)
                     /gene="rpsD"
                     /locus_tag="VBD85_001939"
     CDS             complement(1733..2344)
                     /gene="rpsD"
                     /locus_tag="VBD85_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO7134460.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT          929 a          476 c          427 g          739 t
ORIGIN      
        1 ttgccataaa ataggtaaat ctgaacacta cacttattct caaaaattcg ttgattttat
       61 tatctctgaa atagaaaaag accctcaaca ttttgttgaa agtctgaaaa aatctaaata
      121 aaaaagataa cccctggcac ataggaatgc tcagcccgaa ggcttacccc attctgggac
      181 ccagcgttaa tccttcacaa gttatctttg ttaactatat tatatcacgc acgttctaaa
      241 tgtaaaggat tttgttagct aaccagtaag ctaactttgt caaaaacccc tgaaaaacag
      301 ctttaaatca tccataatcg cattttaacc tttaaccagg taatttttac cgacttctcc
      361 aaaacaaacg aaataagagt cttctcgtaa gctctggcat gatataaacc taaaatccct
      421 ttaataatag cttgcctgct gatggaaagg tttatgatca tgaaaataac tgaagtaaaa
      481 aagaaagatg gtacggtaat ttatcgtgcc agtatttatc ttggtactga taaagtaaca
      541 ggtaaaaaag taactactaa aataacagga cgaactaaga aagaagttag agaaaaagct
      601 aagcaagaag ctatcgagtt tataaaaaat ggttctactc gcttcaaagc tacttccata
      661 acaagttatc aggaacttgc aaccttatgg tgggatagtt acaaacatac cgtaaaatac
      721 aatactcagc tagctactga aaagctgtta accgttcatg tcataccaat ttttggagca
      781 tataagcttg ataagttaac gacaccactt atacagtcta tcatcaataa actagctgat
      841 aaaactaata aaggggaaag aaaagcttac ctccattatg acagaataca cgcgctgaat
      901 aaacgtatac tacagtatgg cgttatcatg caagctatac cattcaaccc tgcgcgtgag
      961 gtcattctcc ctcgcaacac taagaaagct aacactaaaa gagtaaagca tttcgaaaat
     1021 gatgaactaa gaacattttt caactactta aacaatctag ataaaaataa atacagatac
     1081 ttctatgaag tcacacttta taaattttta ttagctacag gttgtcgcat taatgaagcg
     1141 ttagctctaa actggtcaga tatcgacttg gataatgccg ttgttcatat cacaaaaacg
     1201 ctaaattaca aacaagaaat taatagtcca aagtcaaagt caagctatcg tgatattgac
     1261 atagatagtc gaacagttac gatgcttaag cagtatagac gacgacagat tcaagaagca
     1321 tggaagttag ggcgttcaga aacagtggta ttttctgatt ttatccataa gtacccaaac
     1381 aatagaacct tacaaactcg attaagaaca cattttaaaa gagcaaatgt atcgaatata
     1441 ggctttcatg gttttcgtca cactcacgct agtttattgc tgaacacagg tatcccctac
     1501 aaagaactcc aatatagatt aggtcattct actctatcta tgactatgga tatttatagc
     1561 catttatcaa aagagaatgc aaaaaaagct gtctcattct ttgaaacagc aattaactca
     1621 atatagtaaa aaatcaaaag ttagcaaaaa agtaagcaaa cccagaaatc aatgttttaa
     1681 gataagacaa aaagcccact gttgtaggct ttctgtaaga tatatcttaa aattaaagca
     1741 ttttgttgta gaattcaacg acaagtgctt cattgatttc tgggttaatt tcatcgcgtt
     1801 ctggaaggcg agtcaatgaa ccttcaagtt tttcagcatc aaatgatacg aaagctggac
     1861 gtcctaaagt agcttcaaca gcttcaagga ttgcaggtac tttcattgat ttttcacgaa
     1921 ctgaaataac ttgtcctgga gtaacgcggt atgatgggat atcaacacgt ttaccatcaa
     1981 caaggatatg accatggtta acaaattgac gagcttggcg acgagttgtt gcaagtccta
     2041 aacgataaac aacgttatca agacgacgct ctaaaagaac cataaagtta aaaccaagag
     2101 ttccttcttt tgctttagta gcttgtacga acaagttacg gaattgtttt tcaccaagtc
     2161 cgtatgaaaa acgaagtttt tgtttctcag ctaactgcaa gccatattct gaaagcttgc
     2221 tacggttatt aggaccgtgt tgaccaggta cgtagttacg acgtgccaat tctttacctg
     2281 tgcctgtaag tgataaacca aggcgacgtg attgtttcca tgatggacca gtataacgtg
     2341 acatctaaat gtcctcctct aaaataattg aggaaataac gatttaaaaa atcctgattc
     2401 gtgcagggga ttttcgccta aacagcaaag ggtactagat tagttaacca cctgttgacg
     2461 agcttcatat ttttctgctg ttatttcaca caagctatat tatatcatga aaataatatt
     2521 gaatcaagtt tttatatctc ttaaacctta aatacaaata gatatctaat a
//
LOCUS       DATRSD010000140         2545 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRSD010000140 DATRSD010000000
VERSION     DATRSD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.141"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..539
                     /locus_tag="VBD85_001940"
     CDS             <1..539
                     /locus_tag="VBD85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134461.1"
                     /translation="ELREFNIGDNVYSVNPDYAEKNHSTVITDLPQKDNETNIITTED
                     RKKFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDLIGAVSSAQ
                     PHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAQYHIPTTVFNGWFRY
                     STLNEDEKNSWLSILNIL"
     gene            512..1192
                     /locus_tag="VBD85_001941"
     CDS             512..1192
                     /locus_tag="VBD85_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134462.1"
                     /translation="MAKHPEYFVNFRHKEDNVTWWNDFNKLDDKDYGTVKWVNGKSHK
                     IESWKFTDDGKLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELVKEKASELFAKTKVMPPGITDLSPEELA
                     DTYSEGGVREDTIVTPIETFFDEKVTNAQEITTSYINLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            1177..1563
                     /locus_tag="VBD85_001942"
     CDS             1177..1563
                     /locus_tag="VBD85_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134463.1"
                     /translation="MVTVLDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHLLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            1597..1878
                     /locus_tag="VBD85_001943"
     CDS             1597..1878
                     /locus_tag="VBD85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134464.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQRALVRIVYAPEIVEEADSIKMT
                     YSDKIDNVSETAKSSIKTIANQIDSGLVGQFGEKVKAQVDLNKGSYDDL"
BASE COUNT         1015 a          338 c          453 g          739 t
ORIGIN      
        1 aagaacttcg tgaattcaat attggagata atgtatattc agttaacccc gattatgcag
       61 aaaaaaatca ttcaactgtc ataacggatt taccccagaa agataacgaa acaaatatta
      121 ttacaacaga agatagaaaa aaatttaagg tattaaaaac ttctcctgac gatatgtctg
      181 gctatcaggg tatggcagtt gcaccaatta tcaagggtaa agtagattat aacagtgttg
      241 cagttatatc cgccgcaacg gattcaagta attataaaga tttaattggc gcagtttcat
      301 ctgctcaacc acatcaaagt tcgactcagt taaaatcagc tgataaattt ttaaaagacg
      361 tacagtcaca tgacaaatgg actgtgactc agctttcagg ctattctcaa tctgcatata
      421 tgttaaaatt aggtgctcaa tatcatatcc cgactacagt atttaatgga tggtttaggt
      481 atagcacttt aaatgaagat gaaaaaaatt catggctaag catcctgaat attttgtgaa
      541 ttttcgacat aaagaggata atgtaacttg gtggaacgat tttaataaac tagatgataa
      601 agattatggt accgtgaaat gggtaaatgg taaaagtcat aaaatagaga gttggaaatt
      661 tactgatgat ggtaagttaa aagatgaaaa aggtaacatc gtaaatccta agtcaccagc
      721 tgttcagtct gttctttatg aagaagttca ttttcaaaaa gctaaagcta aattaaaaaa
      781 atctggaggc aaacttagtc atagtgaaaa agtatattta gattctgaac aggctatatt
      841 tattgctaac ggtttaacca cggcatctca aacagcttct gatgatatca agaagaatgc
      901 tgaattagtt aaagaaaaag catcagaact atttgcaaaa actaaagtga tgcctcctgg
      961 tataacagat ttaagtccgg aagaattagc tgatacatat agtgagggcg gagttagaga
     1021 ggacaccata gtaacaccaa ttgaaacttt ctttgatgaa aaagttacta atgcacaaga
     1081 gattacaact tcatatatta atcttcaaaa acaaattgaa tctggtgtcc aaaaattgct
     1141 agaagaggac tccaaattag caggagaatt taaggaatgg tcacagtact agatcaaaat
     1201 gaaaacaaaa ttaatagtct aaaaataaac ctagggaaat tggaagataa ttatcatcgc
     1261 gaaaatatgg agatagaaga taaattatat agccttgagg ataaaaagaa cgagttagag
     1321 acttatttac aagaagtcta tgaagagaca agtcaccttt tgagacaaaa tgattcagat
     1381 ggttcgactt ttatgagttt aaatcgaata attgaaagtt atcaaatgga attaattgat
     1441 gaatatgaaa actataaaca gactatcatt gtccaagagg aaaatagtcg gacacagttt
     1501 ctaaaagacc gagtaaaatt agaaaatgac atttctagtt tagaaggaag aaaatactac
     1561 taattcagat ggactataaa gtgaaagagg caataaatga gtttaatagg cgctgtattg
     1621 aaaggaacca aagaagacaa agaattcaaa aagcaaattc aaagagcttt agtaaggatt
     1681 gtctatgccc ctgaaatagt agaagaagct gattctatta agatgactta tagtgacaag
     1741 attgataatg tttctgagac ggccaaatca tcaattaaaa cgatagcaaa tcaaatagat
     1801 tcaggacttg taggacaatt tggggaaaaa gtaaaggcac aggtagattt aaataaaggt
     1861 agttatgatg atttataatc gagtgaaaat gttgaggaag cttactatcc tgatagttat
     1921 tatagatatg aaaataatca agttggatta gaagacataa cattttacat gaaggataac
     1981 aaagtttatt atttacattt tggtaaagca atagataaaa atgaagctaa aaatgataag
     2041 tatctcaatc aaattataaa taattaattt ctttatttta taagccccga aaacaaattt
     2101 tttgataacc ttttgataac ccctccgcga acatattgta atacatagtg cacttttggg
     2161 atataaattg ttgattttta gctagtttat accatataac accatatatt acagttgtca
     2221 gagaaaacca ggtttaaaaa aagcccgtaa agcatcacaa tcctcaaaac gttaatatat
     2281 acctatatat cagcgattgg aagcacttca tggttcacac catggggtgt tttttgtgta
     2341 atattcaaaa attgtcctaa agtcattttt aaaacttcta aatacttgag aaaccattgt
     2401 aaatttaaat aactacttta aatagcacaa aggcaatgac aaagttagat gagatgattc
     2461 tattttaatt tataattgaa aaaaagagta tatgatggag gaattttgta tggtaagtac
     2521 tcaatatatg aaagtaacct tacga
//
LOCUS       DATRSD010000141         2415 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRSD010000141 DATRSD010000000
VERSION     DATRSD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.142"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..243)
                     /locus_tag="VBD85_001944"
     CDS             complement(<1..243)
                     /locus_tag="VBD85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134465.1"
                     /translation="MTEEQEYKQYIYNNLIIQICESLNICSREFFIIKYNLSLDEINH
                     INNVFKEIVTKNILDYTIFKNKIQEGISRFNSDDVFQ"
     gene            1302..1634
                     /locus_tag="VBD85_001945"
     CDS             1302..1634
                     /locus_tag="VBD85_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134466.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKEE"
     gene            1636..2208
                     /locus_tag="VBD85_001946"
     CDS             1636..2208
                     /locus_tag="VBD85_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134467.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRGDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAKPDTSEIVIAYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGSDLHHMISNHGIDYIKSHPGQFRKNRKI"
BASE COUNT          897 a          300 c          434 g          784 t
ORIGIN      
        1 ttggaataca tcgtcggaat taaatcttga aataccttct tggattttgt ttttaaatat
       61 ggtgtagtct aagatgtttt ttgtaactat ttctttgaaa acattattga tgtgattgat
      121 ttcgtctaga gataaattat atttgatgat gaaaaattct ctggaacaaa tgtttaaact
      181 ttcacatatt tgaattatta aattattgta aatatactgt ttatattcct gttcttctgt
      241 cattaacccc cctactctaa agcaagacga atccaataag ctatggttga agctgttatt
      301 tctgccattt cggaagttgt caaggcgttt ttcacttgtc cttcaactct tccgtgggct
      361 aaatcgcttc agtatagtaa atttctcaag accggcataa cggtgttgag agatgctccg
      421 acagatacag ctgcatctcg accgatatat ttaccgacta aattgacgat tttgtttctg
      481 tttcttatct agggtttggc agtatctcct ttctaaccta aataaatttt aaatgaaatg
      541 tttttggttt cattttacca tttttgtaaa tttaatttta catatacact attatgtggt
      601 gaatatgata agactggaaa tatttttaaa tcttctgaag aatggactta gaataacaaa
      661 aactgtgtaa atcagatcat tcctgatgcc tatcattatt ccaatcaagg ttaatcagaa
      721 gttgttaaca agctgattaa taattatctt aatgaaatag attacggata tacgtaatag
      781 aagtagggcg tcaagatatt aaaaaatagg aatttatttt ttcagggaca aatgatggga
      841 caaatcctct ttagacaagc aaaaaagcct ttagataaag gctttttgat gtatagtatg
      901 acacccccta caagatagaa ctaaagaccg ataaaatttg aaaagggctg atattaagca
      961 tttaagattg tttttaatag ttatctgtct ataaggaatg tcgaaaaatg aattttgagt
     1021 gggcaaaaag tgggacaata ataatgatag aatatcgaaa cttcatgcaa attataatgt
     1081 tgagaagaat cagtataaaa atacgaattt cactactagt aagaaataaa aagaaagcgt
     1141 tatctttgaa tttttattat aatagaatga ggtaattcaa ctaatttaaa agaagttatc
     1201 tatatttgaa tggtatagaa aaaacagagg tttatttgct tatgaaaaaa cgtatatggt
     1261 atttgataat aataatcaca gtaattttag gaggactagc catgaaaaac ttatttgcaa
     1321 caacagaagc atcatcaagg aaacaggaac aagatagaat tgtcaattac ataaaacaac
     1381 atgttgagtt aacaaatggt aatcaaataa aaaaaattga gtttatcgac tttcaaaaaa
     1441 atgagatgac aggtacatgg ggaatttcta ctaaaattaa tgaacaattt tcgattagtt
     1501 tttctgaaga tagaattggt ggtaaactta gagcattagg atatcaaccg aatgaaatag
     1561 gtttttcaaa ggacatcaat agtaataatc aaaatgttaa tgatattgaa gtgatttata
     1621 tgaaggaaga atagaatgat ttcagaggaa agttacagat atttagttga ggatgcttat
     1681 cgagttgatt caaaaaaagt aaaaatacct ctgaaaagag gtgatattgt agggaacagt
     1741 gactatgtta ttattgaacc acctatagat aatacttcaa atggtatgca agctatggta
     1801 gttgctccga taaaagaagg aatgacagct aaaccagata ctagtgaaat agtgattgct
     1861 tatgcaggta ctaatttagg tgagcgttta gacatagcta cagatgttga aatggttgct
     1921 ggaggggata catatttact tgctgaccct aaaaccaaga cttttaggaa atctcaggga
     1981 aaatcagcac tagagtatgc cgaaaaaata tcgagtaaat atcctaattc agaaattact
     2041 acaaccggtc actccttagg ggaatcagag gcactatatg tcgctttaaa aatgggttgg
     2101 atgaatgttg gctataacgg ttcagacctt catcacatga tatcaaatca cggaatagat
     2161 tatataaaat ctcatccggg acaatttaga aaaaatcgaa aaatatgagt tgtttgagtt
     2221 aaaattttaa ctatggagat agagtcctgt attatttgat tttgtagtaa actagttagg
     2281 aatttattca taggatatta taaaggagtt aatgatgaag aaaaaatatt ggatattact
     2341 aatattacta ttaacagttt tatcaggagg cggatatatg gtttttaaac aacatacaga
     2401 aaaagaaaaa atgat
//
LOCUS       DATRSD010000142         2404 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRSD010000142 DATRSD010000000
VERSION     DATRSD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.143"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..471)
                     /locus_tag="VBD85_001947"
     CDS             complement(<1..471)
                     /locus_tag="VBD85_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7134468.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGRPFFTELSTFSTKEELIHFFVQ
                     DRFKFIEKNNDLIKILMQELLINSETSTIFTKLINSTDPNITKIFNCLSEGNSLN"
     gene            complement(495..1592)
                     /locus_tag="VBD85_001948"
     CDS             complement(495..1592)
                     /locus_tag="VBD85_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134469.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILEIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1595..2311)
                     /locus_tag="VBD85_001949"
     CDS             complement(1595..2311)
                     /locus_tag="VBD85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134470.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALYESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          789 a          369 c          383 g          863 t
ORIGIN      
        1 attcaacgaa tttccttcac ttaaacagtt gaaaatttta gtaatgtttg gatcagttga
       61 atttattagc ttagtaaaaa tagttgatgt ttcactattg attaataatt cttgcatcaa
      121 tattttaatg agatcattat ttttttcaat aaatttaaaa cggtcctgta caaagaaatg
      181 aattagttcc tctttagtag aaaatgtaga taattctgta aagaaggggc gtccaatagt
      241 ttgtacgatt aactcgagaa taaagaccaa aagcttgtct ttagtttcaa aatatttata
      301 tatagttgct tggctaactt ctgcattttt agctagttga gcagtggaag ttccatggaa
      361 gccttgtgag gcaaagagct ctatagcgga taaaataact ttttgttttc cttttggcat
      421 ctcttggtca ttaatatact gttcaataga aaaaataata ttatttttca tacttagttc
      481 cttttcaaat ggtattatac tttacggtat cgttttagtc caaagatatt tgcgatggtt
      541 aaaataatta aaaatagaag aagtaccaga agatttgaag aaacgttagg taatccttga
      601 ccgtacataa ttatttttgt aagtgcatca ccagagtagg agagcggtag aattttaccg
      661 actgtctgag cccaacttgc catattttct agagggatta ttcctgaaaa gaataattga
      721 ggcattataa ttaagggaat aaattgcatc atttgaaatt ctgattttgc tagagtagac
      781 attaagattc ctaatgataa ggcaaccaga gccaaaataa aattgacaat gatgacacta
      841 aaaatactac ctacaacttg aatatctagt agccaaatag ttgataaaac gataactatc
      901 gtttgaataa tttctagaat gccgtatgat aacatatatc caaatacaat atcactacgt
      961 ttaacaggag ttgcaaggag tcgatcaagt gtaccagaag tacgctcttt taataaagcc
     1021 attccagaaa ttaaaaaaac aaagaagaag accataaatc ccattagtat tggtatcatt
     1081 tttgcaaaaa agcctgtatt tttatttccg taattatatt tttctttgat atatttggag
     1141 cgagtttgta aggattgtgc tagtttcgga ttcttattag ctaaaatttt aacttgcgaa
     1201 atcagttcct tactgttcat tgtattaaca gcggttttaa aagcttgtct tgttaaagtc
     1261 gtctttgaag aatctgtatt cgcatagaag acagtataag atttattgtc ctccgaaata
     1321 agagcatcaa ttttatttga tttgagtgct tttttagcag atgagttaaa tttaaatgat
     1381 ctcacttgaa tatgcttaat attatctaaa tttgaaacga ccttcgtgtt aacgttaata
     1441 gttccaatct taacttttgt attactattc gcagaaaaca taacattcat caaaaacata
     1501 attaaaatag gtgctaaaaa catcatagca agtgttcttt tatcacgaaa cagttctttt
     1561 ataacctttt cagttattgc aataattctc atgattattc tccttcagct tttaagaaaa
     1621 cttcctcaat agtactcaca ttaaattgtt tttttaaatg taatggagta tcaaaggcaa
     1681 taatgtttcc acgtaatagt agtgcaacct tacttgttaa ttctgcttca tccataacgt
     1741 gggttgtaat aaagatagaa cgtccttcat ccttaatatt aattagctct tgccagattt
     1801 ttctcctcaa ggatggatca attccaacgg taggttcatc taggattaaa actgtggggt
     1861 ttccaagtag ggcgatggct agagaaagcc gtcttttcat acctcctgag taacctgaga
     1921 caaatttatc aagttggttt tctagatcta ctactttaga aatatgagtt atctgctgtt
     1981 ttaattcagt tttttgaata cctttcattt ttccaaagaa taataaattt tctaagccag
     2041 ttaaagactc gtataaggca tcagattgag ccatatagcc aatttgattt aaaatattac
     2101 gatctggcat ttgagtatca agaacaagag ctgttccctt atctgctttt tccatgccaa
     2161 gcatagtttt aatcaaggta gatttccctg ctccagaggg tcctattaat ccaattattt
     2221 cgcctttaaa cacctccaaa ttaatattat ttaaaacggt ttctgaggca tatgcttttt
     2281 gtagtttttt taaatcgatg acttttttca tgataacttt ttgctcctct ctcagtgagt
     2341 gagtgtttac tcacttattt gatatcatat attatagtgt gtaaagacag gcttgtcaat
     2401 aaaa
//
LOCUS       DATRSD010000143         2362 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRSD010000143 DATRSD010000000
VERSION     DATRSD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.144"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..1288)
                     /gene="gtfB"
                     /locus_tag="VBD85_001950"
     CDS             complement(<1..1288)
                     /gene="gtfB"
                     /locus_tag="VBD85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO7134471.1"
                     /translation="MINLFDSYTQSSWDLHFSLIKSGYINPTIALNDDGFLPDDVTSP
                     YLYYTGFAKTGAGRPLYYNELRVPDTWEIIGFSSGADIVDLGVKKGRIIYANPNHKRL
                     IKEVDWFDEQGRVILKDRFNKFGFCFAQTFYNADGQAIQTSYYNKDRQEVISENHMTG
                     DYILNDNNQFKVFKSKVEFVINYLQEAKFNLDRIFYNSLSTPFLVSFYLNRLESKDVL
                     FWQEPLVDDIPGNMRLLLNNPSPNTKIVIQSYEAYANAMRLLTDEEQKQVSFLGFMYP
                     LKETEKLHNQALILTNSDQIEALESLVTSLPNLTFNIGALTEMSSDLMNFGKYDNVVL
                     YPNITTNQIQYLSNICAFYLDINHHNEILSAVRSAFEHQQLIFAFEETSHQIRFVSPK
                     NIFPKKDIFTFISHLQPLIGNKCNIEKALKQQLEDCH"
     gene            complement(1281..>2362)
                     /locus_tag="VBD85_001951"
     CDS             complement(1281..>2362)
                     /locus_tag="VBD85_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7134472.1"
                     /translation="YTRTFSEYYYPKDGVAHLYQRRFYNEDESTAFLEMVENSSSRFI
                     INGRLLPSKVAFFDYFLESMTFTSKDIILLDRGTDTAQSLLRHGKPAKLGTVVHAEHF
                     SENAVTADTILWNNYYDYQFTNANRFDFFITSTDKQTELLEQQFKQFTNHNPRIITIP
                     VGSIDNLKMPMDNRRPYSILTASRLASEKHVDWLVRAVIRIREILPEVTFDIYGSGGE
                     EEKIRNIINAANATEYIRLMGHKNLSNVYQNYELYLTASKSEGFGLTLLEAIGAGLPL
                     IGFDVRYGNQTFIKDGENGYLIPRFDMDDEEAIVEAFKEKVLQLFQQDQKALREASYA
                     IAEGFLTNEVEGKWYNLVKELVQDD"
BASE COUNT          788 a          421 c          367 g          786 t
ORIGIN      
        1 catgacaatc ttcaagctgc tgttttaatg ctttttctat attacattta ttgccaatta
       61 gtggctgcaa gtgcgaaata aatgtaaaaa tatctttttt tggaaaaata tttttaggtg
      121 atacaaaacg aatttgatga ctagtctctt cgaaagcaaa aattaactgt tgatgttcaa
      181 aagcagagcg aactgctgat aatatctcat tatgatgatt aatatcaaga taaaaagcac
      241 aaatatttga taaatattgt atttgatttg ttgtgatatt tgggtagagt acaacattat
      301 catatttgcc aaaattcatg agatcactag acatctcggt caaagcacct atgttaaaag
      361 tcaaattagg aagagaagtc actaaacttt ctaatgcttc gatttgatca gaattcgtca
      421 aaataagagc ttggttatgt aatttttctg tctctttaag aggatacata aatcctaaaa
      481 agctaacttg tttttgttct tcgtcagtta ataacctcat agcattagca taagcttcat
      541 aactttgaat tacaatttta gtgtttgggg aaggattgtt caataataag cgcatatttc
      601 ctggaatatc atctacgaga ggctcctgcc aaaagagaac atctttactc tctagacgat
      661 taaggtaaaa gcttactaaa aatggtgttg acagagaatt atagaagatt ctatctaagt
      721 taaattttgc ttcttgaaga taatttatga caaattcaac tttagattta aaaaccttaa
      781 actgattatt atcatttaaa atataatccc ctgtcatatg gttctctgat ataacctctt
      841 gcctatcctt attataatat gacgtctgaa tcgcttgacc atccgcgtta taaaaggttt
      901 gtgcaaaaca gaacccaaac ttattaaagc gatcttttaa aatgactcta ccttgctcat
      961 caaaccagtc tacctctttt attagacgct tatgatttgg gtttgcataa ataattctgc
     1021 cctttttgac acctaagtca acaatatcag caccactact aaaaccaata atttcccatg
     1081 tatcaggaac tctaagttca ttgtagtaaa gtgggcggcc agcacctgtc tttgcaaaac
     1141 cagtgtaata taaatagggt gatgttacgt cgtctggtaa aaaaccatca tcattcaaag
     1201 ctatagtagg gttaatataa cctgatttta tcaaggaaaa atgtaaatcc caacttgatt
     1261 gagtgtaact atcaaataag ttaatcatct tgtaccaact ccttaactaa gttataccat
     1321 tttccttcta cttcatttgt taagaatcct tcggcaatgg cgtaagaagc ttctcgtaaa
     1381 gccttttgat cctgttggaa taattgtaac actttctctt taaaagcttc tacaatagct
     1441 tcctcatcat ccatatcaaa tcgaggaatt agataaccat tttctccgtc tttgataaaa
     1501 gtttgattac cataacgaac atcaaaccca atcaaaggaa gtcctgcgcc aatagcttca
     1561 agtaaagtta agccaaaccc ctcactttta gaagctgtca aatataactc ataattttga
     1621 taaacattcg agagattttt atgtcccatc aatcgaatgt attccgttgc attggctgca
     1681 tttataatat ttctaatttt ttcttcttct ccacctgatc catagatatc aaaggtcact
     1741 tcaggaagaa tttctcttat cctaataact gcacgtacta accaatctac atgtttttca
     1801 ctagctaggc gtgaagctgt caaaatagag tacggacggc gattgtccat tggcatttta
     1861 agattgtcaa ttgagcctac cgggatagtt ataattctag gattatgatt tgtaaattgt
     1921 ttaaattgtt gttccaaaag ttctgtttgt ttatcagtgg aggtaataaa aaaatcaaat
     1981 ctattagcgt tagtaaactg ataatcgtaa tagttattcc ataaaatagt gtcagcagtc
     2041 acagcattct cactaaaatg ttccgcatga acaacagtcc ctagcttagc gggtttacca
     2101 tggcgcaaaa gactctgagc ggtatcagtt cctctatcca aaagaataat atcttttgaa
     2161 gtgaaagtca ttgattccaa aaaataatca aaaaaagcta ctttggatgg taaaagtctg
     2221 ccattgataa taaaacgact tgagctattt tctaccatct ccaaaaaggc tgtactttca
     2281 tcttcattgt agaatctacg ttgataaagg tgagctacac catctttagg atagtaatac
     2341 tctgagaaag tacgtgtata ac
//
LOCUS       DATRSD010000144         2232 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRSD010000144 DATRSD010000000
VERSION     DATRSD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.146"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..258
                     /locus_tag="VBD85_001952"
     CDS             <1..258
                     /locus_tag="VBD85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HEO7134473.1"
                     /translation="ILIQVVFFIYFLLKKINESPDERFYSNLALSASLMFLISIMLLI
                     LIAIYLNIYGTLELKSGYLYIMVGLLLLMFAVTYYFLEGRR"
     gene            261..479
                     /locus_tag="VBD85_001953"
     CDS             261..479
                     /locus_tag="VBD85_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134474.1"
                     /translation="MGKETKIISSLKQVREDIGMTQQELAIRIGVRRETIGHLENNRY
                     NPSLEMALKIVKIFDMKIEDIFQLRKED"
     gene            1052..1612
                     /locus_tag="VBD85_001954"
                     /pseudo
     CDS             1052..1612
                     /locus_tag="VBD85_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1712..>2232
                     /locus_tag="VBD85_001955"
     CDS             1712..>2232
                     /locus_tag="VBD85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134475.1"
                     /translation="MNNLDTLYCKLAMCNTVLTERLRLQPVELTNVNDFLEFSSDSET
                     VFYMQRYKANTVEEAQVVLANVCMKSPLGIYAMIEKESQKMIGIIELEIRDEFSAEFG
                     YILNKNYNGKGYMTEACSKLMSIGFEHLDLERIYARFDINNKKSGNVMERIGMKKEGE
                     LRHLAKNPKGEWKT"
BASE COUNT          807 a          239 c          412 g          774 t
ORIGIN      
        1 attttaatac aagtagtttt cttcatttat tttctattaa aaaaaattaa cgaaagtccc
       61 gatgaacgct tttatagtaa tttagcattg tcagcttctt tgatgttttt gatatcaata
      121 atgttattaa ttcttatagc tatttacctt aatatatatg gaacgttaga gttaaaatca
      181 ggctaccttt atattatggt aggtttatta ttactaatgt ttgctgttac atattacttt
      241 cttgagggac ggagataata atggggaaag aaacaaaaat tatcagttct ttaaaacagg
      301 ttagagaaga tatcggaatg actcaacaag agttagctat tcggataggt gttaggagag
      361 aaacaattgg tcatttagaa aataacaggt ataatccttc tctagagatg gcacttaaaa
      421 ttgttaagat tttcgacatg aagatagaag atatttttca attaaggaaa gaggattaag
      481 gtgaaggaag taatttgttc gttttgtggg gaaaagattt tataacttat aaataaaata
      541 aggggtacgc aggattgctt cctcttaata ggatattagc tactggccta gaagtacatc
      601 atgatatttg tagaaattgt ggtactattg ctagaagtta tataaatgac ttggagaaat
      661 tgaaataggt atataaaata tattggttac tcaaaaatca ctaaaatctc aaaatttctt
      721 agtttttaac ctgatttcca aaacacaaat cttgttaaat cagtgtttgt ttactttaaa
      781 aagtgcttgg tagcactcaa aatccagtgt cctcacggac gttccggttc gaccccggcc
      841 gcctgtatac tttaaaagat aaggtttcta gacctcgtct tttttgtttt gttagataat
      901 ttattgtgaa aagttaccta aaattactta tatttgtttt ttattattgt attttttata
      961 atgtagttaa taacttttca aggaggtaat attatggaaa ttttaattga aacacactta
     1021 tttatttact ctggaagaaa ggaaataaaa attgaataaa ttttttttag aatcagagag
     1081 acttattata aggccactag aagaaagtga ttatgatagt tggtatacta gttttatgaa
     1141 tcgtggacca tctttaactc catttgatga tgggatactt gatatgtcta tgtgtgacat
     1201 taattggttt aaaaatttag taaatttaca aaggaaagaa tgggaaagtg acatagtata
     1261 tatatttggt gtttttctaa aaagcggtga gcacgtagga atgttaaata ttgttaccct
     1321 tgcaagagca gatatgcaat gaggagaatt gggatatgtt tttcataatc aattttggag
     1381 taatggctat gcatttgagt caatcttggc attacttaat agtacttatg aaaaattggg
     1441 ttttcatcat attgaagcac agattactcc tgggaatgaa cgatcggaaa agttggttag
     1501 aagattggga ttaacctatg agacaactcg aaaagatttt tcatttgaga atggaaaatg
     1561 gacagataaa ctgatttata gtataaattt acataataat aatttagaat aattttatag
     1621 acttacagag aaatttaaag cttataatat tgtatttttg gagattattg aagtggttta
     1681 tagataatta aggaaggaat attgattaga aatgaataat ttggatacac tatattgtaa
     1741 attagcaatg tgcaacacag tactaacgga aagattacga ctccagcctg ttgaattaac
     1801 taatgtaaat gattttttag aattttcatc tgattctgaa acagtattct atatgcaacg
     1861 ttataaagct aataccgtag aagaagcaca agtcgtttta gcaaatgttt gtatgaaatc
     1921 accattagga atttatgcaa tgattgaaaa agaatcacag aagatgatag gtattattga
     1981 attagaaatc agagatgaat tttctgcaga atttggttat attttaaata aaaattataa
     2041 tggaaaaggt tatatgacag aagcttgttc aaaattgatg tcaattggat ttgaacatct
     2101 agacttggaa agaatttatg ctagatttga cattaataat aaaaaatctg gaaatgttat
     2161 ggaacggata ggtatgaaaa aagaaggcga gcttcgtcat cttgcaaaaa atccaaaagg
     2221 tgagtggaaa ac
//
LOCUS       DATRSD010000145         2082 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRSD010000145 DATRSD010000000
VERSION     DATRSD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.147"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            146..355
                     /locus_tag="VBD85_001956"
     CDS             146..355
                     /locus_tag="VBD85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO7134476.1"
                     /translation="MPRKVNKVIYVLLALFLGEFGLHKFYAGKTGTGILYLIFCWTFI
                     PGFIGVVEGILAILKPADQDGNFYI"
     gene            1048..1389
                     /locus_tag="VBD85_001957"
     CDS             1048..1389
                     /locus_tag="VBD85_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134477.1"
                     /translation="MDIFSEEFKNELRFIVKDTVSDIVTKAIKNGSFNSTFTIDVAND
                     AFLSQKFCMSKSSVGTIRREMRDFPSYTKFLRNGGSLVTVKGFDEYLQYRGSWEWKKE
                     KAKLRTKKRTR"
     gene            1416..>2082
                     /locus_tag="VBD85_001958"
     CDS             1416..>2082
                     /locus_tag="VBD85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEO7134478.1"
                     /translation="MENLSTRLVDKSIEAFIMGLEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAEMLNRNQSIYFKDNPDRTLSLEGVIKKIYSDDSTRIRLNLERIIELRNISTHY
                     ITEDYELKYAPLFQACVLNYVNELQRFHSRDVTKAISQNFLTITATYEPLSNEQIRLK
                     YPAEIAEKFIQQANAIDVLVTEYNSDKFAIGIKQNLYITKKKSEADFIVSIANQSPSQ
                     VAIL"
BASE COUNT          723 a          312 c          366 g          681 t
ORIGIN      
        1 ctgaaatatc tgattgggca gaagaagagt taccattcta accccaccag aagactacca
       61 cgtgccagta ttttcaaggg ttttatctat ataaataaaa ccatgttatc tgtatttaaa
      121 cgctttatac aaaggagaaa aatttatgcc aagaaaagta aataaagtaa tttatgttct
      181 attagctctg tttttaggtg aatttggctt acataaattt tatgccggta aaacaggaac
      241 aggaatccta taccttattt tctgttggac atttatccct ggattcattg gagttgttga
      301 aggaatctta gcaatcctaa aaccagccga tcaagatgga aatttctata tataaagaat
      361 tattatcttc cacagctttt actgtattcc gtatctgaaa aagattcacg ctaattcagc
      421 aatcaagctg ttcttgcttt tggttatctg gtagggtggc aataaggtag agagcttctg
      481 ttccgatatt tcctaacgtc gggaaatttg gtaattcatg agctattttc atcattcact
      541 ttgcaaaagt ataatcgatt tccaacttat gcaactactc ctccaataaa agcaatggta
      601 gttagaaacg tttgtctctt taatgatgtg ataatttagt attcttatct tcggggtggg
      661 gggtcgtgcg ataaaaaaag caaatatttg gatagtcgac ccttcccacc ggtttcaaaa
      721 cttggattga acaacatttt ttaatgacgg ggggtaacct taaagatact aaattagaaa
      781 aaattctgaa aacttgtggt taagtatttt gttgatgtta ataaaattgt acggtttgat
      841 tttggagtgc gtgaaatatc ttgagattac cttagcttat gtaacacgaa aggcttataa
      901 tataggcttt tcgatagaaa aatatcttga gattatcttt ttgaaaggaa caataaatat
      961 gaatattaac cttcttgaag gaattgaaat tgaatataag tccgctgacc ttgctaaaac
     1021 cttatcgctt ttataggagt aatactcatg gatattttta gtgaagaatt taaaaatgaa
     1081 cttcgattca ttgtgaaaga taccgtttct gatatagtca caaaagccat aaaaaatgga
     1141 tcttttaatt ctacctttac gattgacgtt gctaatgacg cttttttatc tcaaaagttt
     1201 tgcatgtcaa aaagttctgt tggaactatt agaagagaga tgagagattt ccctagttac
     1261 acgaagtttc ttagaaatgg cggatctctt gtaactgtca aaggttttga tgaatacctg
     1321 caatatcgcg gtagctggga atggaagaaa gaaaaagcta aattaagaac gaaaaaaagg
     1381 actcgttaag gtcctaaaaa ttagaaagga aatatatgga gaatttaagt acgaggttag
     1441 ttgataaaag tattgaagct tttattatgg gacttgaaat atacaataaa ccaacgataa
     1501 agtaccgaat tgagggcttt agtttcttta tttgtaatgc ttgggaattg atgcttaaag
     1561 ctgaaatgtt aaatcgtaat caatctatct attttaagga taatcctgat agaacactaa
     1621 gcttagaagg tgttataaaa aaaatatact cggacgacag cactagaatt cgacttaatc
     1681 ttgagcgcat tattgaacta aggaatatta gtactcacta cataacagaa gattatgaat
     1741 taaagtatgc tccacttttc caagcttgtg tacttaacta tgtcaatgaa cttcaacgat
     1801 ttcattcaag agatgttaca aaggccattt cccaaaattt tttaactatc actgcaactt
     1861 acgaaccatt atctaacgaa caaattagat taaaatatcc tgcggaaatt gctgaaaaat
     1921 tcatccaaca ggcaaatgcc atagatgtct tagtgacaga gtataattct gataaattcg
     1981 ctattggtat taaacaaaat ctttatatta ctaagaaaaa atctgaagcc gattttatag
     2041 tatcaattgc aaatcaatca ccttctcaag ttgctatttt aa
//
LOCUS       DATRSD010000146         2080 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRSD010000146 DATRSD010000000
VERSION     DATRSD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.148"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            228..1160
                     /locus_tag="VBD85_001959"
     CDS             228..1160
                     /locus_tag="VBD85_001959"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO7134479.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLEAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(1204..1401)
                     /locus_tag="VBD85_001960"
     CDS             complement(1204..1401)
                     /locus_tag="VBD85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134480.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(1598..1873)
                     /locus_tag="VBD85_001961"
     CDS             complement(1598..1873)
                     /locus_tag="VBD85_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO7134481.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(1976..>2080)
                     /locus_tag="VBD85_001962"
     CDS             complement(1976..>2080)
                     /locus_tag="VBD85_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HEO7134482.1"
                     /translation="NLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
BASE COUNT          682 a          349 c          318 g          731 t
ORIGIN      
        1 agagtattca attactaaag aaaatcttac gccgaacata aaaaataagc ttacataagc
       61 ttatttttta tgcattatag aaagcatcat ttatttcttt actattattg tttatacaaa
      121 caataatgtt cgttatttag cgaaatttta ttaccattat cattattttc ttgcagctct
      181 attaatcaag tgataaaata gtaaaaattt atcttcagga ggatattatg gtatcgctaa
      241 aaacggaaat tgctggtttt agttttgaca attgtttgat gaatgcagcg ggtatttatt
      301 gtatgactaa ggaagagtta ttagctattg aaaattctga agctggttcc tttgttacaa
      361 aaacaggtac ccttgaagct agagagggaa accctcaacc acgttatgca gatactgact
      421 ggggatcaat taattctatg ggactcccta ataaaggtat tgactattat cttgatttcg
      481 tgactgaatt acaagatcaa gacaatagca aaaatcacgt tttatcatta gtaggtttat
      541 caccagaaga gacacatatt attcttaaaa aggttgaaaa cagtagttac aatggcctaa
      601 tagagttaaa tttatcgtgc ccaaatgttc ctggaaaacc tcaaattgct tatgattttg
      661 aaatgactga cctcatcctt tcagaaatct ttagctatta tcaaaagcca ttgggcatca
      721 aattaccacc ttactttgat atcgttcact ttgatcaagc agctacaatt tttaacaaat
      781 atcctttagc ttttattaac tgtgttaatt ctatcggaaa tggacttgtt attgatgatg
      841 aaactgtcgt tataaagcct aaaaatggct ttggaggtat cggtggggat tttatcaagc
      901 ctaccgcctt agctaacgtc catgctttct acaaacgttt aaatccttca attaaaatta
      961 tcggaacagg tggtgttaaa aacggccgtg atgcctttga acacattcta tgtggagcta
     1021 gtatggtcca aatcggaacc gctttacaaa aagaaggacc agaaattttt cagcgtgtaa
     1081 gcagagaatt aaaagaaata atggctgata aaggctatca atcattggag gattttcgag
     1141 gacaacttaa ttacctttaa aaataataaa agagcaaacc ttgtttgctc ttttatattg
     1201 attttaccaa tctttatcaa attcacgttc ttcacgttcc tcgggtgtta aatcatcctt
     1261 tacaggcttt gttatgatgt ttccttcatt atctacttta ggttttatcg tattcatata
     1321 taactttaac agagctatct ttataatagc gataataaca aaagcaataa ggataattaa
     1381 tagtatagct ttttctgaca ttctctttcg caacctctct tttaacttat cttttaggaa
     1441 ttataacatc acagttaaga taattcaagg gtatgctcta acacatttga aactctaaat
     1501 tcctttaatc tctctagtca tttattgagt attggatata aaaaagaacc ttgtcgctat
     1561 caataaacat tgatatgaca aggtttttct agacgaatta tttaacagcg tctttaagag
     1621 ctttaccagc tttaaatgct ggaacttttg atgctgcaat ttcgatttct gcaccagttt
     1681 gtgggttacg acctttacga gctgcacgtt cacgaacttc aaagttacca aaaccgatta
     1741 attgtacttt ttcaccttca gcaaggtaat ctgctacagc tgcaaaaact gcatctactg
     1801 ctgctgctga atctttttta gttaattctg tagcttctgc tacttttgca attaaatctt
     1861 gtttgttagc catgttaaca aatcctccaa ataaattcta aacttataca acataagttt
     1921 taacagatta tatgatatct aaaaaaaggc ttaaggtcaa gtatttaacg tcattttagg
     1981 gctttttctt aaaaatatcg aaactaaaat tatcgtttac taaatcaatt gcagtcgctt
     2041 tcaaataaat accttcttta ttttttaaat cttgcaaatt
//
LOCUS       DATRSD010000147         2072 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRSD010000147 DATRSD010000000
VERSION     DATRSD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.149"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(34..1068)
                     /locus_tag="VBD85_001963"
     CDS             complement(34..1068)
                     /locus_tag="VBD85_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7134483.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWVIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFIQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKEYRLSPLKQ
                     LSVYYFFLHPLFIGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            complement(1297..>2072)
                     /locus_tag="VBD85_001964"
     CDS             complement(1297..>2072)
                     /locus_tag="VBD85_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO7134484.1"
                     /translation="IKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNSTYILIVLFTLF
                     LSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILAYALILTVFADY
                     FGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGLLFIASLNFFIS
                     SITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTSALLLKLSRNGS
                     HGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
BASE COUNT          812 a          351 c          335 g          574 t
ORIGIN      
        1 actataactt taaactttct cttatatgca tcactatttc gaaagagata atggaataat
       61 tttttgatag ggtatcttat taacaacctt tattatgagt agagcacaag catgtgttaa
      121 tgccaaggtc accacaaaaa tcacttttcc ttgatggtgt gcaactaagg aagttgattt
      181 taaacagtaa gacactatgc caataaaaag agggtgaagg aagaaataat aaacactcaa
      241 ctgttttaaa ggacttaaac gatattcctt aaacagtgat gtacgtatac tccagttaaa
      301 taagaatact gcacataaag gagctaataa gaagaaattt ttatccaacc cttgattaaa
      361 ataaataaga acattctcta aagctagaag taaaaggaag agaatatact taagaccacg
      421 cgatttggta aataggtcag tattaaaata atcatagagt aaatatcctg ctagaagata
      481 aactggagtg taaaataatc cgtttcgtgt tgtctgaaaa attgacatat aagtggacat
      541 caggggataa aattttgttg actgtataaa gacagaatag gtctctactg ctccaataca
      601 atagagtaca acaactgtga taaatgtccc cctagttcct acatacttca ataatccttg
      661 aataatgacc caacctagaa taacagcagg gatgtaccaa agggtatgtg acatacctaa
      721 atacaaaaat gccacaataa accccggcaa caaatagatc ttcgctatat ttagagattc
      781 aaaaaagaaa taaccataag gcaaataaat gatagtccac atgctatact ttttaatcag
      841 tttacgaaag taggagtgat tacacaaacc ttgctgtata cgacctctca aaaagaaagc
      901 agtacaaatc aaaaaataag gaacagccat ccgacctagg aaacttttaa aggtaaaatg
      961 aatgacatcc tgactaaata acctcccact atgaactaaa atgaccagta tggaaaaaag
     1021 gtattgtagc aaactaatca ttgatgtatt atgctgctta ttatccacgc actgacttct
     1081 cttcatcctt aacattactt tctataagtt atcattacta tgataccaat ctcttcagaa
     1141 gaatatctca attatcccaa aaagtcattg aaacgcctta aatggtatct gataattatt
     1201 gttaatacct tatcaaccat attaggaaat aaaaaagctg atttcgcaaa agaaaatcag
     1261 cttagaataa tttcttgttc agataaccag tgggaactat aaacttcttc gtcgcattaa
     1321 acgataggta taagctatca ataacactgc aattaacata cgtagactaa tcagacagat
     1381 ttcattagca gaaaaactat caaagaaaaa gccatgacta ccattacgtg ataattttaa
     1441 aagtaaagct gaagtgtctg tcgttgatac tgagaaaata gttaataact tgtttatcac
     1501 acctttatta aacatagcca ttaaaaagct aggagcaaaa aaggcaagcg ttgaaacagc
     1561 aaaagatgtt aatggcgaat ttgtgatact tgatataaag aaattaagac tggctataaa
     1621 aaggagtcca acaaattgta agacaataat atgaacaaaa gcagttgata ttgtcatagg
     1681 tacaggaaat cccataatat tagccaaagg tgatgtccag tataaattca gttgaacgct
     1741 tgtctcccaa ccactaaaac caaaataatc tgcaaatacc gtcagaatga gggcatatgc
     1801 taggataaat gtggaaatac cgatggtcat tgtactagct aacttagccc aggtcaactg
     1861 accacgtcca taccgagtcg tcagtagtat aggattaatc ttcttagccc tatctcctga
     1921 aaaaagagga gctgataaga atagagtaaa taagacaatt aaaatataag tactattact
     1981 tacttcaaaa agttgattcc aaggagcaaa gttacctagc tttagttgtt ttagtggtag
     2041 cgcagaaccc aactgttttt gatctttaat at
//
LOCUS       DATRSD010000148         1979 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRSD010000148 DATRSD010000000
VERSION     DATRSD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.150"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..160
                     /locus_tag="VBD85_001965"
     CDS             <1..160
                     /locus_tag="VBD85_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134485.1"
                     /translation="GGSLINKSSFMLNITTLAESYLILISIIVLSVVMASSLILFKKP
                     KEILSKIS"
     gene            170..826
                     /locus_tag="VBD85_001966"
     CDS             170..826
                     /locus_tag="VBD85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134486.1"
                     /translation="MDILEIKNVNYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKVQTGKILFKNEDIEKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSVDESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKELAQDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            826..>1979
                     /locus_tag="VBD85_001967"
     CDS             826..>1979
                     /locus_tag="VBD85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134487.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFIIEMVFISIPALLGSYFLAQYTADKLGNNILNKVTGD"
BASE COUNT          812 a          229 c          346 g          592 t
ORIGIN      
        1 cggtggaagt ttaataaata aaagcagttt tatgttaaac ataacaacac ttgcagaaag
       61 ttatttaata ttaataagta ttattgtttt atcagttgta atggcctctt cattaatatt
      121 atttaagaaa ccaaaagaaa tattatcaaa aataagttag gagcaaataa tggatatatt
      181 agaaataaag aatgtaaatt acagttacgc aaattctaaa gaaaaagttt tgtcaggagt
      241 aaatcaaaaa tttgaacttg gaaagtttta tgcgatagta gggaagtcag gaacaggaaa
      301 atccacactt ctttccttac ttgcaggact tgataaagtt caaacaggaa aaatcttgtt
      361 taagaatgaa gatatagaaa agaaaggata tagtaatcac agaaaaaata atatatcttt
      421 ggtatttcaa aattataatt taatagatta tttatcgccg attgaaaata ttagactagt
      481 aaataaatca gtagatgaga gtatcttgtt cgaattaggt ttagataaaa aacaaataaa
      541 aagaaatgtt atgaaattat ctggtggtca gcaacaaagg gtagctattg ctagggcact
      601 ggtatcagat gccccaataa tactagctga tgagcctacc ggtaacctag acagtgttac
      661 tgctggagaa ataattaata tattaaagga attagctcaa gatagaaata aatgtgtaat
      721 agttgtaaca catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg
      781 taaaaagttg aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca
      841 tttgcttatg taactagaaa aagcttaaaa tcattaatta ttatattagt tattctatca
      901 atggcaactt taagcatcat tagtttatct attaaagatg ccacagatag agcttctaag
      961 gaaacatttg ctaatataac aaacagtttt tctatggaaa taaatagaca agtaaatcca
     1021 ggaacaccta ggggtggagg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca
     1081 aacagtatag actcctatgt aaaaagaata aacagcgtcg cagacctagt tgatcatgat
     1141 ataattgaaa ctcaagatac acttgcaaat caatcacctg aaagagcaaa gaattttaag
     1201 agaacagtta tgttaacagg ggttaatgac tcagcaaaag aaacaaaatt tgtatcagaa
     1261 gcatataagt tagtagaagg aaagcattta gaaaataaag ataaaaataa aattttaatg
     1321 cataaagatt tggcaaaaaa aaacaatctc aaagttggag ataaaataaa aataaaatct
     1381 aatttgtttg atgctgacaa tgaaaaagtt gcaaatgaaa ctgtagaagt agaaattaaa
     1441 ggtttatttg atggtcataa tagcggtggg gtaagtgcag cacaagaact atacgaaaat
     1501 acactaatca ctgacgttca ttcagctgct aaagtttatg gtaatacaga agatacagcc
     1561 gtataccaag atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa
     1621 gatcttggga aactagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat
     1681 tatcctgcgc tgcaacaatc tatatccggt atctattcta tttcaaacaa attatttgtt
     1741 ggctcattaa tatttgcagg agttgtagtt tcattattac ttttcttatg gatgaatgcc
     1801 agaaagaaag aaatagcagt attactatca ttaggtatat ctaaattaga aatttttggt
     1861 caatttataa tcgagatggt atttatatcc attcccgcac ttcttggatc ttatttctta
     1921 gctcaatata cagctgataa gctagggaat aatatattga ataaagttac tggcgatat
//
LOCUS       DATRSD010000149         1909 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRSD010000149 DATRSD010000000
VERSION     DATRSD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.151"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..903
                     /locus_tag="VBD85_001968"
     CDS             <1..903
                     /locus_tag="VBD85_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7134488.1"
                     /translation="CILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFINIPI
                     ELFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSVITYSMKFYKYS
                     HLIAKRFLDIMGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFR
                     SMRVDAEQIKKDLLVHNQMTGLMFKLDDDPRITKIGKFIRKTSIDELPQFYNVLKGDM
                     SLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDEIVKLDVQYINE
                     WSIWSDIKIILLTLKVVLLGTGAK"
     gene            927..1376
                     /locus_tag="VBD85_001969"
     CDS             927..1376
                     /locus_tag="VBD85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO7134489.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1376..1867
                     /locus_tag="VBD85_001970"
     CDS             1376..1867
                     /locus_tag="VBD85_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO7134490.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFVNKVKTMYNFDIVVDIERLQNVVYEGTMNRPFLETNRSNFIEEFKVILKELCD
                     ENQ"
BASE COUNT          669 a          242 c          382 g          616 t
ORIGIN      
        1 tgtatcttgg actcctctga aaaggattgt tatgatttga aacataactc gttaaggata
       61 ataaacaaag atgctcttac ttcagagtta acctgcttaa ctgttgatca agcttttatt
      121 aacataccca ttgaattatt tggtaaatac caaatacaag atattattaa tgacattgaa
      181 gcaatgggag tgattgtcaa tgttaatgta gaggcactta gctttgataa tataggagaa
      241 aagcgaatcc aaacttttga aggatatagt gttattacat attctatgaa attctataaa
      301 tatagtcacc ttatagcaaa acgatttttg gatatcatgg gtgctattat aggtttgctc
      361 atatgtggca ttgtggcaat ttttctagtt ccgcaaatca gaaaagatgg tggaccggct
      421 atcttttctc aaaatagagt aggtcgtaat ggtaggattt ttagattcta taaattcaga
      481 tcaatgcgag tagatgcaga acaaattaag aaagatttat tagttcacaa tcaaatgaca
      541 gggctaatgt ttaagttaga cgatgatcct agaattacta aaataggaaa atttattcga
      601 aaaacaagca tagatgagtt gcctcaattc tataatgttt taaaaggtga tatgagttta
      661 gtaggaacac gccctcccac agttgatgaa tatgaaaagt ataattcaac gcagaagcga
      721 cgccttagtt ttaagccagg aatcactggt ttgtggcaaa tatctggtag aaataatatt
      781 actgattttg atgaaatcgt aaagttagat gttcaatata tcaatgaatg gtctatttgg
      841 tcagatatta agattattct cctaacatta aaggtagtct tacttgggac aggagctaag
      901 taaaggtaag gtttgaaagg aatataatga aaatttgtct ggttggttca agtggtggtc
      961 atctagcaca cttgaacctt ttgaaaccca tttgggaaaa agaagatagg ttttgggtaa
     1021 cctttgataa agaagatgct aggagtattc taagagaaga gattgtatat cattgcttct
     1081 ttccaacaaa ccgtaatgtc aaaaacttgg taaaaaatac tattctagct tttaaggtcc
     1141 ttagaaaaga aagaccagat gttatcatat catctggtgc cgctgtagca gtaccattct
     1201 tttatattgg taagttattt ggttgtaaga ccgtttatat agaggttttc gacaggatag
     1261 ataaaccaac tttgacagga aaattagtgt atcctgtaac agataaattt attgttcagt
     1321 gggaagaaat gaaaaaagtt tatcctaagg caattaattt aggaggaatt ttttaatgat
     1381 ttttgtcaca gtggggacac atgaacagca gttcaaccgt cttattaaag aagttgatag
     1441 attaaaaggg acaggtgcta ttgatcaaga agtgttcatt caaacgggtt actcagactt
     1501 tgaacctcag aattgtcagt ggtcaaaatt tctctcatat gatgatatga actcttacat
     1561 gaaagaagct gagattgtta tcacacatgg cggcccagcg acgtttatga atgcagtttc
     1621 taaaggaaaa aaaactattg tggttcctag acaagaacag tttggagagc atgtgaataa
     1681 tcatcaggtg gatttcgtta ataaggtaaa aacaatgtat aattttgata tcgttgtaga
     1741 tattgaaagg ttacaaaatg tagtctatga ggggacgatg aatcgtccgt ttttagaaac
     1801 taacagaagt aattttattg aagaatttaa ggtaatatta aaggagttgt gtgatgaaaa
     1861 tcaataaaaa taaaaattat ttttttgaag taatttttat catactttt
//
LOCUS       DATRSD010000150         1856 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRSD010000150 DATRSD010000000
VERSION     DATRSD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.152"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..220
                     /locus_tag="VBD85_001971"
     CDS             <1..220
                     /locus_tag="VBD85_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134491.1"
                     /translation="EYSELEQEYQRISALIDAKFPQGKSSLAEKLHKELIWDLDGVKY
                     DEGISKRTALKKLEGKINEYTKKYSYLF"
     gene            complement(272..457)
                     /locus_tag="VBD85_001972"
     CDS             complement(272..457)
                     /locus_tag="VBD85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134492.1"
                     /translation="MDNCTLEFPYSFENNFKPILSAFTSNEMPSYPMLPLCLQKAPPS
                     LIGQKKTPQKGVFLVSI"
     gene            529..1254
                     /locus_tag="VBD85_001973"
     CDS             529..1254
                     /locus_tag="VBD85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134493.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEADQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            1256..>1856
                     /locus_tag="VBD85_001974"
     CDS             1256..>1856
                     /locus_tag="VBD85_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7134494.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGGIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVIQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQSILKFFSLFTNVKGISTSVKRRSFL"
BASE COUNT          645 a          228 c          350 g          633 t
ORIGIN      
        1 tgagtattca gaactagagc aagaatatca aagaatttca gctcttattg atgctaagtt
       61 tccgcaaggg aaatctagtt tagcagaaaa attacataaa gaactaattt gggacttgga
      121 tggtgttaaa tatgatgagg gtatttctaa gagaactgct ctaaaaaaat tagaaggtaa
      181 aatcaacgaa tatactaaaa aatacagtta tttattctga taattgagtt tgatagtttt
      241 atagcctaat attctgatta ctttgcattt attatattga tactaaaaag acaccttttt
      301 gaggtgtctt tttttgtccg atgagtgatg gaggtgcttt ttgtaaacat aatgggagca
      361 tggggtagct tggcatttca ttagatgtaa aagcggacaa aataggttta aagttatttt
      421 caaaactata agggaattct aaagtacaat tatccataag aatagacctt ccctttctta
      481 ttttgttata atagttaata aagtatatca aagatagaga gaatgacaat gttaaaaatt
      541 gaaaatgtta caggggggta tgttaatatc ccagttttaa aaaatatttc ttttgaagta
      601 aatgatggag agttagttgg attaattggg cttaatggtg caggaaaatc aaccactatt
      661 aacgagatta ttggtatttt gagaccctat caaggagata ttacaattga tggtattagt
      721 cttgaagccg accaagaact ttatagaaag aaaataggct ttattcctga gacaccaagt
      781 ctctatgagg agctaactct cagggaacat ttagaaacgg tagctatggc gtatgatatt
      841 gccaccgatg aagttatggc acgtgcgcaa aaactattag agatgtttcg tctaacagat
      901 aaattagact ggtttccaat gcatttttca aagggaatga aacaaaaggt tatgattatt
      961 tgtgcttttg ttgtatctcc aagtttattt attgttgatg aaccattttt aggtttggat
     1021 ccactagcta tttcagattt gattaatctt ctagcagaag aaaaagctaa agggaaatct
     1081 attttgatgt caacacacgt attagattct gccgaaaaaa tgtgtgatcg ttttgttatt
     1141 ttacataaag gagaaatacg ggcagttgga accctagagg aattacgggc tatttttgga
     1201 gacagtaatg ctaatttaaa tgacatctac atagctctga caaaagaggt gtgatatgaa
     1261 aaagttattt aataaacgtc gaagtctctt tttaactcaa aatagtaaat atttgagata
     1321 cgtctttaac gatcattttg tattagtatt aatgtttttg tcaggttttt tgttatatca
     1381 gtatagccag ttactaaaag actttccaaa aacacattgg ccaattatcg tgattgtgtc
     1441 aatcattatc ttaatgttac tagcgatggg gggaatagcc agctatttag aaccggctga
     1501 taaacaattt ttacttataa aagaagaagc aataaaggaa attataaatt ctgctaaaaa
     1561 acggacttat atcttttggt tggttattca aaccttattt ttagtattaa tatcaccaat
     1621 tttgataaag ttaggcttgt cagtttttat gattacattg ttaatttttg gtctaggaat
     1681 aatcaaatgg ttggtaataa cttacaaggt aaaggtcttt tataacaatc aaaatttgaa
     1741 ctgggatgca gctataaatc atgagcagga aagaaaacag agtatattaa aatttttctc
     1801 tttgtttaca aatgttaaag gaatttcgac aagtgtaaaa cgtcgttcat ttttag
//
LOCUS       DATRSD010000151         1750 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRSD010000151 DATRSD010000000
VERSION     DATRSD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.153"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..332
                     /locus_tag="VBD85_001975"
     CDS             <1..332
                     /locus_tag="VBD85_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134495.1"
                     /translation="SVYSIISGIAIDKVIIVIISSFVIAIFSAFFIIDILEIIFHLKI
                     DSLFLIAYLFFAISLGFGLLKVLTESQFSLLSAFIAFPLFLIFPKLFEKIVNNRKINQ
                     KDNKNQK"
     gene            725..1513
                     /locus_tag="VBD85_001976"
     CDS             725..1513
                     /locus_tag="VBD85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134496.1"
                     /translation="MARGRGKLTPQEIEALRFFSSKLNDLLNETSTKQVELSKATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADYFGLMKSDIDPRFAPRKKKHELKIPTSPLVNKIT
                     KTVVELNVPRKQQVLDYATEQLKEQKNKITSIEETLYEYKVYEKLSAGTGYGYFGDGN
                     YDTVFYDEQLDYDFASWVFGDSMEPTYLNGEVALIKQTGFDYDGAIYAVEWDGQTYIK
                     KVFREKGGLRLVSLNKKYKDKFAPFSEDPRIIGKVVGNFMPKEY"
     gene            1522..>1750
                     /locus_tag="VBD85_001977"
     CDS             1522..>1750
                     /locus_tag="VBD85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134497.1"
                     /translation="MGLFNKNEKASKKSMVDIYLEQHGINDFPEEYKNAIKNIVNDLS
                     SHGLAKASMILSFSKIEEQAKVGYLSTIINQN"
BASE COUNT          620 a          275 c          272 g          583 t
ORIGIN      
        1 attctgttta ttctataatc tctggtattg ctatcgacaa agttatcatt gtgataattt
       61 catcttttgt tattgctatt ttctcagcat ttttcattat cgatatactg gaaattattt
      121 ttcatctcaa aattgattct ttatttttaa ttgcttattt attttttgca atttcactag
      181 gttttggact tcttaaagtt ttgacagaat cacaatttag cttgttatct gcctttattg
      241 ctttcccttt atttcttatc tttcctaaac tttttgagaa aatagtaaat aacaggaaaa
      301 tcaatcaaaa agacaataaa aatcaaaaat aaataaacca aaaactcttt aataacaatc
      361 tcctctcttt cttgaaagaa agtaaccact atgatatagt aagcataacc cctttaaggg
      421 gcgaggattt tcaatcccct atccgattac cacgtaatca gatattttat tttgagccta
      481 aaccaaagaa tttgaatttc gaactttact tctttgtgtt taggtttttt atttcgccta
      541 ctttccatca gtggctacct cctttcgttt tgcttaattc ctcaagcttg gttatattgt
      601 aatatgattt aaatcgtatt ttaatcattt tttcggtaaa aatcgtattt attccaaaaa
      661 tattatttac aaatacgatt taaaacggta ttatataagt ataaaaattc gaaaggaaaa
      721 aattatggca agaggaagag gtaaattaac tcctcaagaa atagaggcat taagattctt
      781 ttcgagcaaa ctaaatgatt tgctcaatga aacaagtaca aagcaagtcg aactctcaaa
      841 ggctacagga ataccaccaa gcactcttac aggttatgtt aaaggaacat ctttacctat
      901 tccaggtaac gtccaaaaaa tagcagatta ctttggatta atgaagtctg atatagaccc
      961 aagatttgca cctcgtaaga aaaaacatga actcaaaatt cctacttctc cgctcgttaa
     1021 caaaattact aagacggttg ttgaattaaa tgttccacgt aaacaacaag tactagatta
     1081 tgctacagag caactaaaag agcaaaaaaa taaaataacc tccatagaag aaacgttgta
     1141 cgaatataaa gtatatgaaa aactctctgc tggtacaggg tatggttact ttggcgacgg
     1201 aaattacgac actgtctttt atgatgagca attagactat gattttgctt cttgggtttt
     1261 cggtgattca atggaaccga cttatcttaa tggcgaagtc gcactaataa agcagacagg
     1321 ctttgattat gatggagcta tatatgctgt cgaatgggat ggacaaactt atattaagaa
     1381 agtatttaga gaaaaaggtg gtttaagatt agtttcttta aacaaaaaat acaaagataa
     1441 gtttgcacct tttagtgaag atccacgtat catcggaaaa gttgttggca attttatgcc
     1501 aaaagaatat tgaggtggga tatgggatta ttcaataaaa atgagaaagc ctctaaaaag
     1561 tcaatggtcg atatatatct tgagcagcat ggtatcaatg actttccaga ggagtataaa
     1621 aatgctatta aaaatatagt aaacgatctt tcgtcacatg gactagcaaa agcctctatg
     1681 attttatctt tttctaaaat agaagaacaa gcaaaagtgg gatatctgtc aacaataatt
     1741 aatcaaaact
//
LOCUS       DATRSD010000152         1733 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRSD010000152 DATRSD010000000
VERSION     DATRSD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.154"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            94..>1733
                     /locus_tag="VBD85_001978"
     CDS             94..>1733
                     /locus_tag="VBD85_001978"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012235.1,HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7134498.1"
                     /translation="MFKLYKKLFKYVPERKTFAYGSMLLAAISTICLMASYWYLWDLF
                     CELLVTKDYSAAIKDSKLIVIYMVSYGVIYFVALMVSHMLGFRLETNIRKTGLKNLIN
                     ASFAFFDTNPSGKVRKIIDDNAGETHSTVAHLIPDNVVAGMLPILMFIMTFAVDYRLG
                     ILLAITIVVGIFQYKSMYGGEEFMMAYSNSLEKMSSEIIEYVRGMQVLKIFGITVRSY
                     KSLLDAILQYSKNTYEYSLSCRKPYVSFQTLFNTYYLICIPCSMYFITKGEQAGMILA
                     KVIFFTCFSGVIFSSFMKVMFASQNNFNAGQVIDRLENLIDGMKNEKLEHGERKEFKH
                     YDIEFDRVFFKYDEKYIIENLSFKLDEGKTFALVGPSGSGKSTIAKLISGFYSLNSGS
                     IKIGGYDIREYSEETLLQNISFVFQNSKLFKASIYENVKMGDQNATREEIMNALHLAK
                     CESILAKFPEKENTIIGSKGVHLSGGEVQRVAIARAILKKSKIIILDEASAAADPENE
                     YELQQAFSNLMKGKTTIMIAHRLSSIRNVDEILFIENG"
BASE COUNT          606 a          200 c          334 g          593 t
ORIGIN      
        1 taaatgaagc taatatttac attcaacacg ttaaaaaatt tttctatgct ggatggaaag
       61 atttgctgag aatataagat ttggagaaga aaaatgttta aattatacaa aaaattattt
      121 aagtatgttc ctgaaagaaa aacattcgca tatggttcaa tgctattagc tgctatttcc
      181 acaatatgtt taatggcttc ttactggtat ttgtgggatt tgttttgcga attattagtt
      241 accaaagatt atagtgcggc tataaaagat agtaaattga ttgtaatcta tatggtatcg
      301 tatggagtta tttactttgt ggcattgatg gtatcacata tgcttggttt tagattagaa
      361 acaaatatca gaaaaactgg gttaaaaaat ctaatcaatg cttcttttgc tttctttgat
      421 acgaatccgt cagggaaagt aagaaagatt attgacgata atgctggaga aacacattct
      481 acagtggcac atttgattcc tgataatgtt gttgcaggaa tgttgccaat tttaatgttt
      541 attatgacct ttgctgttga ttatagactt ggtattttgt tggctataac aattgtagta
      601 ggtatttttc aatacaaatc aatgtatggt ggagaagaat ttatgatggc atattctaat
      661 tctctggaga aaatgagttc agagataatt gaatatgtta gaggaatgca agtattgaag
      721 atttttggga taacggttcg ttcctataaa agtttattgg atgcaattct ccaatactca
      781 aaaaacactt atgaatattc cttaagttgc agaaagccgt atgtgtcatt tcaaacgtta
      841 tttaatacct attatttgat atgtattcct tgcagtatgt attttataac aaaaggagaa
      901 caagctggga tgattcttgc aaaagtaatc ttctttactt gtttttctgg ggttatattt
      961 tcatcattta tgaaagttat gtttgcttca caaaataact ttaatgccgg acaggtaata
     1021 gatcgtttag agaatttgat agatggaatg aagaatgaaa aattagaaca tggtgaaaga
     1081 aaagaattta aacattatga tattgagttt gatagggttt tctttaaata tgatgaaaaa
     1141 tatattatag aaaatttgag ctttaagtta gatgagggaa aaacttttgc tttagttgga
     1201 ccatctgggt cagggaagtc aacgatagcg aaattgatat caggttttta ttctttaaat
     1261 agtggcagta taaaaattgg tggttatgat atacgagaat atagcgagga aacattactt
     1321 caaaatattt catttgtatt tcaaaactca aaattattta aagcctctat ttatgaaaat
     1381 gtaaaaatgg gtgaccaaaa tgcgacaaga gaagaaatta tgaatgctct gcatttggca
     1441 aagtgcgaaa gtattttggc aaaatttcct gaaaaggaga ataccattat aggctcaaag
     1501 ggagtgcatt tatcaggtgg tgaagtacaa agggttgcca ttgcaagagc tattttgaaa
     1561 aaatctaaga ttattatact cgatgaagct tcagctgccg cagatcccga aaacgagtat
     1621 gaacttcaac aagctttttc aaatttaatg aaagggaaaa caaccattat gattgctcac
     1681 agattatctt caattagaaa tgttgatgag atattgttca ttgaaaatgg caa
//
LOCUS       DATRSD010000153         1698 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRSD010000153 DATRSD010000000
VERSION     DATRSD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.155"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..182
                     /locus_tag="VBD85_001979"
     CDS             <1..182
                     /locus_tag="VBD85_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO7134499.1"
                     /translation="TARNIIKQSKAIAVAKFEETSNSTNNLVKLRKSPFENSRLDLAP
                     TYIVEDLLGFELPFK"
     gene            200..1381
                     /locus_tag="VBD85_001980"
     CDS             200..1381
                     /locus_tag="VBD85_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7134500.1"
                     /translation="MANKKYRGVSRDKQGRIYYQTEFKANNITGKRQRKKSYKDQFGN
                     PFRSEKQAYDELCRVRVEFQTKDNNDGYYLSFSQYMEEVYLPYYKQTVQAVTYRTALT
                     HHKMFVEIFGSKRLSEIDVRECEKFRIYLIEKYSPNYAKGVWIRFKHCIGYAERLGYI
                     DEFPCKSLDNPKGKRPDTKFWTLEDLKKVVATFDLRDYEERHRFTAIWLYFFTGLRVS
                     EGLSLIWSDIDFDKKLLHVQSTLEYEGNGVFTRKNPTKTSAGIRWIELDDETLNVLLR
                     WRSIQVENGDDSYVLARFGKPLNKCTLSRILKRQAKKAGVEVITGKGLRHSHDSFLIN
                     VLGKDVLYVSKRSGRVDKATTLNTYSHLYDSKKASGGREISQALHKFGVSSNPTKTPL
                     N"
BASE COUNT          609 a          256 c          322 g          511 t
ORIGIN      
        1 atactgcaag aaatattatt aaacaatcaa aagcgatagc agtagcaaag tttgaagaaa
       61 ctagtaattc tacaaataat ttggtaaaat tgaggaaatc gccctttgaa aattctagac
      121 tagaccttgc acccacctat attgtagaag atttgctagg cttcgagtta ccttttaagt
      181 aaagaaagga aacttgaaaa tggcaaataa aaaatatcgt ggtgtatcac gtgataaaca
      241 aggaagaata tattatcaaa cggagttcaa ggctaataat ataactggta aacgccaacg
      301 caaaaagagt tataaagacc aatttggaaa tccttttcgt tccgaaaagc aagcatatga
      361 tgaactttgt agagtgagag ttgaattcca aacgaaagat aacaatgatg gttattatct
      421 aagtttttct caatacatgg aagaagtgta tctaccttat tacaaacaaa cggttcaagc
      481 ggtcacttat cggactgctc taactcatca caaaatgttt gttgaaatct ttggtagtaa
      541 aagactttct gaaattgatg ttcgagagtg cgaaaaattc aggatatatc tgattgaaaa
      601 gtattcacca aattatgcta aaggtgtatg gattcgattt aagcattgta ttgggtacgc
      661 agagaggctg ggatatattg atgaattccc ttgcaaatct cttgataatc cgaaagggaa
      721 aagaccagat acaaagtttt ggactctaga agacttaaaa aaagtagtcg ctacctttga
      781 cctacgggat tatgaagaaa ggcatagatt tactgccata tggctctatt tctttacggg
      841 tcttcgtgta tcagagggtt taagtttaat ttggtctgat attgatttcg acaaaaagct
      901 cttacatgtt caaagtacac ttgagtatga aggaaacgga gtctttactc gcaaaaatcc
      961 aactaagact tcagctggta taaggtggat tgagttagat gatgaaactc taaatgttct
     1021 tctaagatgg cgttcaattc aagtggaaaa tggtgatgac agttatgtat tggctagatt
     1081 cggaaaacca ctcaataaat gcacattaag tagaattcta aaacgccaag ctaaaaaagc
     1141 aggagtagaa gtaatcactg gaaaagggtt gcgacattct cacgacagtt ttttgataaa
     1201 cgtactagga aaagatgttc tgtatgtttc taaacgttct ggacgagttg ataaggcaac
     1261 taccttgaat acctattctc atttgtatga tagcaaaaaa gctagtggtg gaagagagat
     1321 atcccaagca ttacataagt ttggagtatc atcaaacccc accaaaaccc cactaaacta
     1381 aataagagca agaaaaaccc gattatatca atgtttataa gcattgtgta actgttactc
     1441 ttaaataatc atctattggt tgtttaaagg tgaaagccta gttttaaagt cttttcgtta
     1501 aagttaaggc gattaaaaaa ggaaagattc aattgaatct tccccacaaa cacccccaca
     1561 atattttgtg ggggtgtttt gtttaatatg gatttagaat atcattataa agtatttctt
     1621 gaataattaa acgattttct aattgaagtt ttgtgaataa atttctgcca tgattcttat
     1681 tattaaaagt atctccat
//
LOCUS       DATRSD010000154         1648 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRSD010000154 DATRSD010000000
VERSION     DATRSD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.156"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..834)
                     /locus_tag="VBD85_001981"
     CDS             complement(<1..834)
                     /locus_tag="VBD85_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7134501.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVLGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSIPLIF
                     HLLGHQLLNQLDRIMLVRLYSTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EK"
     gene            complement(831..>1648)
                     /locus_tag="VBD85_001982"
     CDS             complement(831..>1648)
                     /locus_tag="VBD85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2,3-N-acetylneuraminyltransferase"
                     /protein_id="HEO7134502.1"
                     /translation="AIYKRRIEKLFPEIPVLIVDDSDYSKKYKLFYPKRIKQELFSIT
                     NGNKIHIYNDYTQLGYFFHRNAIPYHLMEDGYNSLKHPTSIAPDGLCDKIKGKITNTP
                     RYHGFSKYCKTIEVSSLKDLPTDERMSKFIEKPKDGLFSDLDNKTKESILKIFDIASI
                     DIEIASPSALILTQPLEEHFKSQEEIYQFYKSIVEEYIDKGYHIYIKVHPRDNIDYSN
                     LNATVIQRNIPIELFDFLENIKFDVGITYFSTALDFLNCVNKKIFLYNIKDIK"
BASE COUNT          604 a          278 c          227 g          539 t
ORIGIN      
        1 tttttctttt ctagcatcaa aaaaccatgg aatccaagcc atattaatac tattcaaaac
       61 aatctgaata atcattccaa gcgaataacc gaaactatac attgcaactt ctttagtact
      121 atataagcga actagcataa tcctatctaa ttgatttaat aactgatgtc ctaacaaatg
      181 aaaaatcaaa ggaatagaca cagtgacaat gaaccgaaaa tacttaggat tgtaaaaaga
      241 tttgctatga taataaatat aagcaaaggc aactattcca gttattatag taggaacaaa
      301 agctcccaaa accctagata aaaagtcatc ggaccaatga aaaattaaaa atagagatag
      361 tgcaacagat gaaactgcat tggctaagga taatattaaa ttagccaaac tcctctgttg
      421 ttgtataaaa tactgaccaa agaaagtcac cacatagcta gcgtaacttt gacacaccat
      481 taataaaaaa atagattcag aaaaaccaac taatggtgca agaaaacttc taaatagaaa
      541 agctatcaaa agtataataa gaaaccatat attacctaca gaaaaggcac tcccagaata
      601 gcttttaaat tcttctccgt atttgacccg ggctgaactt aaagaaccgc tcgtctgaag
      661 gccaataaaa agcataataa tattcatcca agcgacatat aaactatatt ggccataaac
      721 ttctgttgag atgacccgag tgtagatggg aagtgtgata aaggctaatc cttgcaccaa
      781 taaattcccg agtgtataaa taaaaccaga ttgaagtaat ttcttagtat tcatttaata
      841 tccttaatat tgtataagaa aattttttta ttaacacaat ttaaaaaatc taatgccgtg
      901 gaaaaatatg taatcccaac atcaaatttt atattctcta ggaaatcaaa taattctata
      961 gggatgttcc tttgtattac tgttgcattg agattagagt agtcaatgtt atctctaggg
     1021 tgaactttaa tatatatgtg gtaaccttta tcaatatatt cttccactat gcttttgtaa
     1081 aattggtata tttcctcttg acttttaaaa tgttcctcta aaggttgagt taatattaga
     1141 gcagatggag aagctatttc aatatctatt gaggctatgt caaaaatctt taaaatagat
     1201 tccttagttt tattatctaa atcagaaaat aatccatcct tgggcttctc aataaactta
     1261 gacattctct catctgttgg taaatctttc aaactactta cttctatcgt cttacaatac
     1321 ttactgaagc catgatatcg gggtgtattc gttatcttcc cttttatctt gtcacaaaga
     1381 ccatctggag cgatactcgt tggatgtttt aaactattat atccatcttc cattaaatgg
     1441 tagggaatag cattcctatg aaaaaaatat cctaattgcg tatagtcatt gtaaatatgt
     1501 attttattgc cgttggttat actaaacaat tcttgtttta ttcgcttcgg ataaaacaat
     1561 ttatatttct tactgtaatc actatcatct acaatcaata caggtatttc tggaaacaac
     1621 ttttctattc ttcttttata tattgcat
//
LOCUS       DATRSD010000155         1572 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRSD010000155 DATRSD010000000
VERSION     DATRSD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.157"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            60..983
                     /locus_tag="VBD85_001983"
     CDS             60..983
                     /locus_tag="VBD85_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7134503.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
     gene            complement(1008..>1572)
                     /locus_tag="VBD85_001984"
     CDS             complement(1008..>1572)
                     /locus_tag="VBD85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7134504.1"
                     /translation="KLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRSVSPILYRLFM
                     ILLAQEVPHLHDYIHNARDDHYDTWKFKELKESNHPVLLAFSERWHDSRLTSKSLAEC
                     LQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSSQAFLDQIIFL
                     AKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT          519 a          267 c          267 g          519 t
ORIGIN      
        1 atattgataa tttagctgat ctaattgaat ttatttacat tctagtttaa actagagtta
       61 tgagaattca acaattacaa tatgttatta agattgtaga aactggtagc atgaatgaag
      121 ccgctaaaca actctacatt acccaacctt cactttcaaa cgctgttagg aaccttgaga
      181 ctgagatggg gatccaaatt tttattcgaa atccaaaagg aataacgtta acaaaagatg
      241 gtatggaatt tctgtcatat gcgcgacaaa ttctagaaca aacagcactt cttgaagagc
      301 gttataaggg tgataataca agtcgtgagc tgttcagcgt ttcttcacaa cactatgcat
      361 ttgtggttaa cgcttttgta gctctcttta atggcacaga catgacccaa tacgaacttt
      421 ttttacgtga aactaggact tgggaaatta ttgatgatgt taaaaatttc cgttcagaga
      481 ttggtgttct gtttcttaat agctataata gagatgtatt aaccaaatta ttcgacgata
      541 attccttaat tgccacaact ctttttacta ctacacctca tattttcgtg agcaaatcca
      601 atcccttagc taatagaaaa aaattaagta tgaaagattt agaagattat ccttatctga
      661 gttatgatca aggcctgcat aattctttct acttttctga ggaaatgatg tctcaaattc
      721 ctcacccaaa atccattgtt gtcagtgata gagcaactct gtttaattta atgattggat
      781 tagatggtta tacagtggca actggtatcc tcaactcaaa attgaacggt gacgaaattg
      841 tcgcaatccc tcttgacgtg gatgatgtta ttgatattgt ctatatccga cacgacaaag
      901 caaacctttc aaaaatggga caaaaattta ttgactattt attagaggaa gtttcgttta
      961 atcataaaaa taaacagtcc tgagattgtc tcagaactgt ttttaaatta ctcaagtatc
     1021 tttataataa gcttgttaac cgtatcgtag tgaaaattaa cagtatcata ctcaacacca
     1081 attacctttg ccaagaagat aatctggtct aaaaatgctt gagaagaaaa ttgagttgtg
     1141 cgatatagtt cttgctcatc aaaaggttta atcaggtgag ccaaaggatt tctgactgat
     1201 ttttcgaact gtcttaattg aatgatggtc gatttcactt cttcatcaag gtcggttaat
     1261 tgtaaacatt ctgcaaggct tttagaagtc aagcgactat cgtgccacct ttcagagaat
     1321 gccaaaagga ctggatggtt tgactccttt aattctttaa acttccaagt atcgtagtga
     1381 tcatctcttg cattatggat gtaatcatgc aagtgaggta cttcttgtgc aagtaaaatc
     1441 ataaatagac gatataaaat aggactaaca ctacggacaa aatctatgat taatccattt
     1501 tttacttttg cttctaaatc catgatgtaa tttgccagca actctaaatc atacgagtta
     1561 tgcaaaagct tt
//
LOCUS       DATRSD010000156         1555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRSD010000156 DATRSD010000000
VERSION     DATRSD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.158"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..514
                     /locus_tag="VBD85_001985"
     CDS             <1..514
                     /locus_tag="VBD85_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7134505.1"
                     /translation="SNEQMSDFEYDKLKYNYPDFCFSKRGIFKKIYRGTKVYRFFKNA
                     RYYSLPPLRKGSQWFTISREFTDWIIGYLDKHPDYQEAFCHSYCGDELFFQTLLAISP
                     FQERVMNNKDDCLMGGRYIIWEADKTSPCDLFAEKVLEYRKEAESALFFRKVSEESDF
                     DVYMHLLNKL"
     gene            530..1483
                     /locus_tag="VBD85_001986"
     CDS             530..1483
                     /locus_tag="VBD85_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134506.1"
                     /translation="MKLLFIVPYFGKFNSYFQLFLNSIATSDLCDLLIVTDDKSKYDY
                     PQNVTIRYTSFEEFRNEVQRKMDFKITLDTPYKLCDFKPTYGYLFEDELLNYQYWGYC
                     DLDIIFGDLDGFLHPILNKGFDKIFELGHCTIIKNESRINRMFLSTVNGVKIGQKALS
                     SSKIEVFDEAYVNSINNIFIENNCNNFLYSLGADIDIWKNNFYITSFKNKKDFVVSDA
                     PSIFYYEQGHLYEFHQYKSVVRRSEYLYIHLQQRKMKVDLDARSTTYLILPNKFVEYR
                     IATNCLLTLKEFKRFISFGKYSFQKYRLYTKLQKQKIKKLL"
BASE COUNT          562 a          183 c          256 g          554 t
ORIGIN      
        1 ttctaacgag caaatgagtg attttgagta tgataaactc aagtataact atcctgattt
       61 ttgtttttct aaaagaggaa tatttaaaaa aatttataga ggtaccaaag tttataggtt
      121 ctttaaaaat gcaaggtatt attccctacc tcctcttcgt aagggaagtc agtggtttac
      181 tatttcaaga gagtttactg actggattat tggatattta gataaacacc cagattatca
      241 ggaggctttt tgtcatagct attgtggaga tgaattattt tttcaaacat tactagctat
      301 ctctccattt caagaaaggg tgatgaataa taaagatgac tgtctcatgg gagggagata
      361 tattatttgg gaggctgata agacatcacc ttgcgattta tttgctgaaa aagtgttaga
      421 atataggaaa gaagctgaga gtgctttatt ctttagaaaa gtatctgaag aaagcgattt
      481 tgatgtttat atgcatctat taaataaact ttaaattaag gagaaaatat tgaaactttt
      541 atttattgtt ccatattttg gaaaatttaa tagttatttt cagttatttt taaactcaat
      601 agccacatca gatctttgtg atttattgat tgttaccgat gataaaagta aatacgatta
      661 ccctcagaat gttactattc gctatactag ttttgaagag ttccgaaatg aagtccaaag
      721 aaaaatggat tttaaaatta ctttagatac tccatacaaa ctctgtgatt ttaaaccaac
      781 atacggttat ctatttgaag atgaattgtt aaattatcaa tattggggtt attgtgactt
      841 agatattatc tttggtgatt tagatggatt tttacatccc atactaaata aaggctttga
      901 taaaattttt gagctaggtc attgtacgat tattaagaat gaatcacgaa taaatcgaat
      961 gttcctttct acagttaatg gggtgaaaat aggacaaaaa gctctaagtt cttccaaaat
     1021 cgaagttttt gatgaggcat atgttaatag tattaataat atatttattg aaaataattg
     1081 taataacttt ttgtacagtt taggagcaga catagacatt tggaagaata atttttatat
     1141 tacttctttc aagaataaaa aagattttgt agtatcagac gctcccagca tattttacta
     1201 tgaacaagga cacttatatg agtttcacca atataagtct gtagtaagaa ggagtgagta
     1261 tttatatatt catcttcaac aaagaaaaat gaaagttgat ttagatgcta ggtctacaac
     1321 ttatctgata ttaccaaata agttcgtaga atatagaata gctacaaatt gcttgttaac
     1381 acttaaagaa tttaaacggt ttattagttt tggaaaatat agctttcaaa aataccgcct
     1441 atatactaaa ttacaaaagc aaaaaataaa gaagttatta tgagatttta atgaaaatga
     1501 agaatattta tatatgttac actttatatc acctattgat atctttatgt aagag
//
LOCUS       DATRSD010000157         1452 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRSD010000157 DATRSD010000000
VERSION     DATRSD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.159"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            54..1409
                     /gene="rlmD"
                     /locus_tag="VBD85_001987"
     CDS             54..1409
                     /gene="rlmD"
                     /locus_tag="VBD85_001987"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7134507.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDVIRQ
                     ALMKFKPEGYENYEIRKTIGMPEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDSLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKDDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
BASE COUNT          490 a          202 c          309 g          451 t
ORIGIN      
        1 tttttacatg ttatggattt atagtatttt cttagttagg gagtaatgaa ctgatgaatg
       61 tggttttaaa acagcgaatt cctttaaaaa ttaaacgaat gggaattaat ggtgaaggaa
      121 taggttttta taaaaaaaca ctaatctttg ttccaggggc cctcaaggga gaagaggtgt
      181 tttgtcagat ttcttctgtt agacgtaatt ttgcagaagc taaactgtta aagataaata
      241 aaaagtctaa aaatagggta gatcccgctt gttctattta taaagaatgt gggggatgcc
      301 aaataatgca ccttcaatat gataagcagc tagagtttaa aacggatgtt attcgacagg
      361 ctctaatgaa atttaaacca gagggatatg agaattatga aatccgtaaa acgataggta
      421 tgccagaacc tgaacactat cgtgctaaat tacaatttca agtgcgttcg tttggaggga
      481 atgtaaaggc aggtttatat gcacaaggga cacatcgatt gattgatatc aaagattgtt
      541 tagtgcaaga tagccttact caggagatga taaatcgagt tgcagaactt ttaggaaaat
      601 ataaattgcc tatttataat gaacgtaaaa tagctggtgt ccgtactgtt atgattcgac
      661 gagcacaagc gagtggagag gttcaactga tttttatcac aagtaaaaga cttgattttg
      721 atgatgttgt tattgaatta gttcgagagt ttcctgagtt aaagacagtt gctgtaaata
      781 tcaacgctag taaaacaagt gatatctatg gtcaaataac agaagttatt tggggtcaag
      841 aatccatcaa tgaagaagta ttagattatg gtttttcgtt atcaccacgt gctttttatc
      901 aacttaatcc taaacaaaca caaattttat atagtgaggc agttaaggcc ttagatgtga
      961 aggatgatga tgatttaatt gacgcttatt gtggagtagg aacgattgga cttgcttttg
     1021 ctggaaaagt taaaagtgtt cgtggaatgg atattatccc tgaggctatt caggatgcta
     1081 aagagaacgc acttcatatg ggttttacta atacacatta cgaagctgga aaggcagagg
     1141 atattatccc taggtggtat agtgagggat ttcgcgctaa tgcgttaatc gtggatcctc
     1201 ctcgcacagg tttagatgat aaattgctaa atactatact aaaaatgcct ccagaaaaaa
     1261 tggtttacgt ttcttgtaat acatctacgc ttgctagaga cttagtaaca ttaacaaaag
     1321 tataccatgt tcactacatc caatctgttg acatgtttcc acatacagca aggaccgagg
     1381 ctgtagtgaa actacaacgt aaggtataat aaagttttct aatcaaaaaa taagagtatt
     1441 tttgggtatt ct
//
LOCUS       DATRSD010000158         1443 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRSD010000158 DATRSD010000000
VERSION     DATRSD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.160"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            144..1427
                     /locus_tag="VBD85_001988"
     CDS             144..1427
                     /locus_tag="VBD85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7134508.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQIDKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLATVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          517 a          262 c          240 g          424 t
ORIGIN      
        1 gtcgtagaga ttaattccta tcattcactg ctttatttta ggataactaa acaatttatt
       61 gtttaaaatg aaaggattta tgaatgtcta ggaaaacatt taaacatata ctatcgattg
      121 gagtttgcac gctcgtacta tcgatgagtc tttattacac tgaaaaagcc cacgctattg
      181 ctggtcctag tgaccgccaa tacgtagaaa acccaaatcc tcacattatt gtaaatgtta
      241 caggtactga tcaaaacgga aatagcattt taccgcatta catcgaagtc aatgtaaaga
      301 tgggacaaac tttaagtaaa gaagaaattc tagattatat tgctcgaaat ttaaactcta
      361 gtgttggagg agaaagtaaa aacgttcaat acagcaacat cgagtttaag gaaagtgctt
      421 atctgaagcg tcaattagat gatggcaaga cagaagaaat agcaattgat aacgacggtg
      481 ttactgtacc taaagacggt ccaaacaaat tttggattga cgttccagta acttgtactg
      541 ttactcctat cgtaacagaa acacatgaag ttcgatgggg gactccagtc gctatatcac
      601 accgtattta ctttgttgaa gaatcttctg gaaaagtttt agatgaatac acaaatctac
      661 acactgctga ttcggaactt aacggttatc gtgttggaga ttatatcaca gactatgcac
      721 tttctaagtc tgcttacgaa gcttttttaa actctcgttt agataaagaa ggttacaaac
      781 ttcaacatcg tattagcacg aatgtacgac aaaaccttca aattgataaa ttgattttca
      841 attatgactt taatgaggaa aatatttact accaaatcgg gaatatccgt ccactattaa
      901 gtcgctcatc agctgaagta gaatctgaca tcattacaga acgctactat gtttctaaaa
      961 atgctaaaag tttagcacgt acagaatcaa ccatttcgat taaaatggtt gatgccaaaa
     1021 ctgaacaacc gctatttaac cacacattaa ctggttatca attggcaact gtctcccatg
     1081 tctataacag actctttgaa gaaaatctta tcccaactac aaaatcagga gaaagatatt
     1141 ttattcaaaa tatgaaaaaa acagctgaac aagaatatac tgtttacctt tcagaaacac
     1201 cttattctaa agagaacgct cccgtaattt cttatgatgc aagacctgtt gattgggatt
     1261 atcactcagg cgcttcagga tcacttgaaa atcagcctaa catctatact gaagaagatt
     1321 caactgaatt tttgggtaat aaaccacaag cagcttgtta tccaaacaaa caatttgctt
     1381 gcgaaaatac tgactctaaa tacaactata gctatttaga aaaatagtta taaccgatag
     1441 ggt
//
LOCUS       DATRSD010000159         1425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRSD010000159 DATRSD010000000
VERSION     DATRSD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.161"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            84..710
                     /locus_tag="VBD85_001989"
     CDS             84..710
                     /locus_tag="VBD85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO7134509.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            722..1060
                     /gene="cadX"
                     /locus_tag="VBD85_001990"
     CDS             722..1060
                     /gene="cadX"
                     /locus_tag="VBD85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO7134510.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIVYYFIKDE
                     EIRDFFNQLG"
     gene            1111..>1425
                     /locus_tag="VBD85_001991"
     CDS             1111..>1425
                     /locus_tag="VBD85_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134511.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDEKLYSFMS
                     DDL"
BASE COUNT          467 a          210 c          242 g          506 t
ORIGIN      
        1 ctaacttatt atctatattt tggtggattt taagttgaca tctacaaagt ttaatgttag
       61 aatacattca aaaatatatt tgaatgaggt attttatgat tcaaaatgtt gttacttcaa
      121 taatcctgta ttctgggaca gccgtagact tacttattat cctaatgtta ttttttgcca
      181 aaagaaaaag cagaaaagac atcattaaca tctatttagg acaatttcta ggctctgtta
      241 gtctaatatt cctaagtttg ctttttgcat ttgtcttaaa ttatattcct agtaaagaga
      301 ttttaggttt actcggtttg attccaattt tcctaggcct aaaagttttg cttttaggag
      361 attctgatgg agaagctatt gcaaaagatg gtttgcgaaa agacaataaa aacctgattt
      421 ttctagtcgc tatgattact tttgcaagtt gtggcgctga caatattggt gtctttgtcc
      481 catattttac caccttaaat ttagcgaatt tgatagtgac tttacttact tttctagtca
      541 tgatttatct cttggttttt tctgcacaaa aattagcaca agtcccttct gttggagaaa
      601 ctttggaaaa atatagcaga tggtttattg ccgttgtcta tttaggattg gggatgtata
      661 tcctgattga aaacaacagc tttgacatgc tatgggctgt gttaggctag gagaaaaaat
      721 tatgaaaaaa gatagtatct gtcaagtgga tgttataaat caacaaaatg ttacaaccgc
      781 aacgaactac cttgaaaagg aaaaagtcca aaaatcactt cgcattttat caaaatttac
      841 cgataataaa cagataaata tcatctttta tctccttgcc gtcgaagaac tctgtgtctg
      901 cgatatagcc tgtttattaa atctcagtat ggcatctgcc tcccaccatc ttcgtaaact
      961 agccaatcaa aacatcttgg acactagaag agaggggaaa attgtatatt attttataaa
     1021 agatgaggaa atcagagatt tttttaatca actaggataa caactatttc tactactttc
     1081 tacttttcca tgattatagg gggattatat atgaaatttt ttgaagaaaa ttattcacaa
     1141 gaaataccta ctcgtatcaa aaatttaaga aaaaaataca atattacaca aagcgaacta
     1201 ggcaatgcag gtcaagttag tcaagttgaa agtggtaagc gaccaattac gagttcgatg
     1261 ttagtttatt taaatgctct aaccgcttcg agctatacgt atatcgtttt tggagagtta
     1321 gatgagttta ttgagaatct gtttcattat tttttcagtt ctattttata tagagattta
     1381 gaggctgtag atgaaaaact atattctttc atgagtgatg attta
//
LOCUS       DATRSD010000160         1400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRSD010000160 DATRSD010000000
VERSION     DATRSD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.162"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(178..543)
                     /gene="rplL"
                     /locus_tag="VBD85_001992"
     CDS             complement(178..543)
                     /gene="rplL"
                     /locus_tag="VBD85_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO7134512.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     SGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(607..1107)
                     /locus_tag="VBD85_001993"
     CDS             complement(607..1107)
                     /locus_tag="VBD85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO7134513.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILIRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNVAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1137..1270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          419 a          272 c          220 g          489 t
ORIGIN      
        1 gactaagggt acccttagcg atatatatac atattatatc ataaataaaa ccccaccaag
       61 atttaatctt ggtggggttt tggtggggaa tgataacatt ctcccgattt tttatcgctc
      121 taagcaattc taaaaccttg atttactagg cttccgcaat caaattgcga tactctatta
      181 tttaagagta actgaagcac ctgcagcttc aagtttttct ttgatttcgt tagcttctgc
      241 ttctgaagca ccttctttaa taactgatgg tgcgttgtca acaatagctt tagcttcttt
      301 aagaccttcg cctgtgattt cacgaacaac tttgataacg ccaacttttt tgtctccagc
      361 agctgttaat tcaacgtcaa atgagtcttt agcagcagca gcttcaccac tagcagcggc
      421 agcagctaca ggagcagcag cagttacacc aaattcttct tcgatagctt ttacaagatc
      481 gttaagttca agaattgtag cttctttaat ttcagcaata atattttcaa tgtttaatgc
      541 cattgttatt tcctccaaaa atatgtttta atataaaatt atgtagctag gctacttgaa
      601 gagtgcttat gccacttctt cgtctttttc tgcaactgct ttgacagcat atgcaacgtt
      661 gcgtactggc gcttgaagta ctgaaaggag cattgaaagc ataccttctt tgtttggtaa
      721 tttagcaaga gcgttaattt cttcaactga agtgaattta ccgtctactg aaccaccttt
      781 gatttcaaga gcttctgcat ctttagcgaa atcgctaata actttagctg gtgcaataac
      841 atcttcgtta gaaaacgcaa ctgctgatgg accaacaaaa agttctttaa ggtcttcaag
      901 acctgctttt tcagctgcac gaattaaaat tgagtttttg ataactttaa actcaacatc
      961 gctttcacga agtgaacgac gtaaattagt atcttgctca acagtaagac cacgtgaatc
     1021 aacaacaacg atacttgctg ctgctttcat tttctcagca ataagttcaa cttgttcagc
     1081 tttttttgca ataattgctt cactcattag ttttacctcc gtttttattt tttgggatag
     1141 gcacaaaaaa atcgcaccta aacctagaca cgaaagtaca atacgttatt ttataatgac
     1201 gttttgtgcc tcggtaggaa tattatgagc actgctcccc tactgtctta ggtcagtttc
     1261 atcagaaact ttaaatagta taacttataa ttctagcatt gtcaataatt tttattattt
     1321 ttctgaaata attttatagt accaaaaaat actaaaatag ttatctttta tatcaaaaaa
     1381 ttaatatttt agtatttatt
//
LOCUS       DATRSD010000161         1316 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRSD010000161 DATRSD010000000
VERSION     DATRSD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.163"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..1100)
                     /locus_tag="VBD85_001994"
     CDS             complement(<1..1100)
                     /locus_tag="VBD85_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO7134514.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSSIIAP"
BASE COUNT          526 a          212 c          212 g          366 t
ORIGIN      
        1 aatggcgcaa taatagatga tagtataaca acaataacaa gctcggaata agtagtgctt
       61 gaaataattt tagcagctaa tccaacttga actataatga gagccatctc gccacgactg
      121 accattccac caccaattgt taaagattct aattttgaaa aattaaagcc tcgaccgaca
      181 aagtaccctg gaattaattt tgataaaaca gccaaagcag taaagattaa tatcgtgtga
      241 aggtgagaca tcattccatc gaatttaaga ggtagtgcaa ttgaagcgaa gaaaattggg
      301 ataaagaagg tgtaactaag aagtgaaatt ttatgttcta ccttatcaac aaaagaagtt
      361 tgtccaatag ctaatcctgc aaaaaacgaa ccaatgatag aactcattcc aactttatcg
      421 gctaaaattg atagagaaaa acagataagg atagcaagaa tagtatattt ctcaaagaaa
      481 tgaacctttt cgattaattt gaaaagagca ggtacaaggt atttcataca aataaataag
      541 aaaacaaaga ataacaattg aatgataatt tgtaatccga tattagaaga gcttccattt
      601 gttgcaataa agacactcag aagcaaaaca gctaagacat catcagcaac agcagcacct
      661 aaaataatag caccagtatc tgtttttaca cgattgtatt cttgtaaaac ttcaactgtg
      721 atagaaatac tagttgcagc aaatacgagc ccgtaaaaga tagcagtatt gatttggaac
      781 ccaaacaagc gtgtcaagaa gtagaagaga gccataggaa caataacacc agtaattgcg
      841 accagtagac tgggcttaag atacttcttt aaaagatcaa aattagcttc aagacccgct
      901 aaaaacatga gtaagataac accaatttct gacaagacat gtaatacctg attttgatga
      961 acaagtccta gcattgcagg tccgatgatg atacctacaa gtagttgtcc aacaacagcc
     1021 ggtattccaa ttctttttga aataagagtt gcaagaacgc ttgccaacaa aatgattgta
     1081 atttgaatga taatatgcat tttttcctcc taatattaat aaaatgatat caatcatatt
     1141 gtactgaata tttagaatac cattttttaa gcctaaaatc taggaatatt tcttttctgt
     1201 ctttttcata ttattatcaa gaaaatggaa aaatatactg aaaaatattc aatttaagaa
     1261 taatgaactt gtaactatcg aactacataa aaatgatatt ttaagtgtaa gtatct
//
LOCUS       DATRSD010000162         1303 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRSD010000162 DATRSD010000000
VERSION     DATRSD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.164"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..575
                     /locus_tag="VBD85_001995"
     CDS             <1..575
                     /locus_tag="VBD85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEO7134515.1"
                     /translation="KQRTEKELIKYLQQHDIDSKIIPQIIDNLKSENWINDKNYVQSF
                     IQQNLNTGDKGPYVIKQKLLQKGIKSKIIESELQAINFQDLASKISQKLYKKYQNKLP
                     LKALKDKLMQSLTTKGFDYQIAHTVIQNLEIEKDQELEEDLIYKELDKQYQKFSKKYD
                     QYELKQRIINALMRKGYQYEDIKSALREYL"
     gene            659..1192
                     /locus_tag="VBD85_001996"
     CDS             659..1192
                     /locus_tag="VBD85_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO7134516.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          544 a          197 c          201 g          361 t
ORIGIN      
        1 tcaagcagag aaccgagaaa gaacttatta aatatcttca acaacatgat attgattcta
       61 aaattattcc acaaattatt gataatctaa aaagtgagaa ttggataaac gataaaaact
      121 acgtgcaatc tttcatacag caaaatctaa atactggcga taaggggcca tacgtgataa
      181 aacaaaaatt attgcaaaaa ggtatcaaaa gtaaaataat tgaatctgaa cttcaagcta
      241 taaattttca agacttagca tccaaaataa gccaaaaact ctataaaaaa tatcaaaaca
      301 aacttccact aaaagcatta aaagataaat tgatgcagag cttaactaca aaaggatttg
      361 attatcagat agcgcatacc gtaattcaaa accttgaaat tgaaaaagat caagaacttg
      421 aagaagacct catttataaa gaattagata aacaatacca aaaattctca aaaaaatatg
      481 accagtatga attaaagcaa cgtataatca atgccttaat gcgtaaagga taccagtacg
      541 aagatatcaa aagtgcttta agagaatatt tataagataa taactctcag acgatgtatt
      601 ttacagaaaa ttatgataaa ctataacaga cgtataaatt gtagaaagtt ggtaggctat
      661 gagattacct aaagaaggcg actttattac aattcaaagt tacaaacatg atggtagttt
      721 acaccggact tggcgtgaca ccatggtatt aaaaacaacc gaaaatgccc tcattggtgt
      781 taatgatcat actttagtaa cagaaaatga tggtcgacgc tgggtgacac gagagcctgc
      841 aatagtatac tttcataaaa aatactggtt taacattatc gctatgatac gtgaaactgg
      901 tgtctcctac tattgtaatc tagcaagtcc gtatatcttg gatcctgaag cactcaagta
      961 catcgactac gaccttgatg tcaaagtatt tgcagatggt gaaaaaagac tactagatgt
     1021 ggacgaatat gaacagcata aagctcagat gaactatcct accgatattg attatatatt
     1081 aaaggaaaat gtaaaaatat tggtagaatg gataaatgag aataaaggcc ccttttcatc
     1141 atcatatatc aatatctggt ataaacggta ccttgaattg aaaaagcgct aactaacaca
     1201 ctaaatagtg tgtttttatt aatatcaaat ttaattacaa tactattgca aaaatatata
     1261 cttaaaataa aaaaagtaga aagtgatctt tctacttttt taa
//
LOCUS       DATRSD010000163         1230 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRSD010000163 DATRSD010000000
VERSION     DATRSD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.165"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(31..459)
                     /locus_tag="VBD85_001997"
     CDS             complement(31..459)
                     /locus_tag="VBD85_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134517.1"
                     /translation="MWNVKTFDNLTTHELFQIYKLRVSVFVVEQDCPYQEVDDKDLIC
                     LHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLGNQLVEHAIKFSEANYPN
                     KPIYAQAQAYLQDFYQSFGFQPVSEIYLEDNIPHLDMVLS"
     gene            complement(493..>1230)
                     /locus_tag="VBD85_001998"
     CDS             complement(493..>1230)
                     /locus_tag="VBD85_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEO7134518.1"
                     /translation="RKAIINPEDINSPIKGFPKTVITCFARETFNRILEELPHREIAR
                     TSVANLEIPIYELEFKGQKIGFFNAYVGASACVAILEDIIVFGMENLIVFGTCGVLDS
                     SIEETSIIIPRSAIRDEGTSFHYSEASSEIAVNTKFHEEFKSFLDRQAVSYTEGKVWT
                     TDGIYRETVDKMLEHKRNGAICVDMECSAIAALSKFRNINHFQFFYSADNLDAEVWEA
                     RSLGNSEDLEKKDKIAYIAMEFAIETF"
BASE COUNT          414 a          252 c          155 g          409 t
ORIGIN      
        1 cttttaattg atagtttaaa tagcatgtat ttatgataaa accatatcaa gatgaggaat
       61 attatcttct agatagattt ctgagacagg ttgaaatccg aaagattgat aaaaatcttg
      121 taaataagct tgggcctgag cataaatagg tttattaggg taatttgcct cgctaaattt
      181 aatcgcatgt tccactaatt gatttcctaa tccctttttt ctaaagtctg gattaacaat
      241 cacacgtccc aaatgcacct tatcatcttc tggtatcaat ctataataag ctgccaattg
      301 accatctacc caattcatcc catgcaaaca aatcaaatct ttatcatcaa cctcttgata
      361 aggacaatct tgttcaacca caaatacaga tactcttaat ttatatattt gaaacaactc
      421 atgtgtagtt aaattatcaa aagtttttac attccacatt gctatcttcc tttcaacatc
      481 atacagtaga ccctaaaaag tctctatagc aaactccatg gcaatataag caattttatc
      541 tttcttttct aaatcctcac tatttcctaa agatcttgct tcccatactt ctgcatctaa
      601 attatccgca gagtaaaaaa attgaaaatg attaatattt cgaaacttag ataaagctgc
      661 aattgctgaa cactccatat ccacacagat tgctccattc cgtttatgtt ccaacatttt
      721 atcaactgtc tcacggtaaa taccatccgt tgtccaaact tttccttcag tgtaagaaac
      781 agcttgacga tctaaaaaag atttaaactc ttcatgaaat tttgtgttta ctgcgatttc
      841 agaacttgct tccgaataat gaaatgatgt cccttcatcc ctaatagcac ttcttggaat
      901 aataatggat gtctcctcaa ttgacgaatc aaggacgcca catgttccaa aaacaattaa
      961 attttccatt ccaaacacta taatatcttc caagattgct acgcaagctg aagcaccaac
     1021 ataagcattg aaaaagccaa tcttttgtcc tttaaattct aattcataaa tgggaatttc
     1081 taagtttgct acactagttc tagcaatttc acgatgaggc aattcttcta aaatacgatt
     1141 gaaagtctcc cgcgcaaaac aggtaataac agtctttgga aaacctttta ttggtgaatt
     1201 aatatcttca ggattaataa tcgcttttct
//
LOCUS       DATRSD010000164         1227 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRSD010000164 DATRSD010000000
VERSION     DATRSD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.166"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            56..>1227
                     /gene="secY2"
                     /locus_tag="VBD85_001999"
     CDS             56..>1227
                     /gene="secY2"
                     /locus_tag="VBD85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO7134519.1"
                     /translation="MKLLYIFEKNIILRKILITFSLIIIFLLGRYVPIPGVLISAYKG
                     QDNNFATLYSTVTGGNLSQVGVFSLGIGPMMTTMILLRLFTIGKYSSGVSQKVQQFRQ
                     NVVMLVIAIIQGLAITISFQYHNGFSLTKLLLATMILVTGAYIISWIGNLNAEYGFGG
                     MTILVVVGMLVGQFNNIPLIFELFQDGYQLAIILFLLWTLVAMYLMITFERSEYRIPV
                     MRTSIHNRLVDDAYMPIKVNASGGMAFMYVYTLLMFPQYIIILLRSIFPTNPDITSYN
                     DYFSLSSIQGVVIYMILMLVLSVAFTFVNIDPTKISEAMRESGDFIPNYRPGKETQSY
                     LSKICYLFGTFSGFFMAFLGGVPLLFALGNDDLRTVSSMTGIFMMITGMSFMILDE"
BASE COUNT          360 a          177 c          222 g          468 t
ORIGIN      
        1 gatataaagt tacttatctt gaagagaaca aattgataga gaaagagaaa ttccattgaa
       61 attactatat atttttgaaa aaaatattat tcttcgtaaa atattgataa cattttcatt
      121 aataatcatt tttttacttg gtcgctatgt tccaatacct ggtgtcttga tttctgctta
      181 taagggacaa gacaataact ttgcgacgct ttattcaaca gtaactggtg gtaatttgtc
      241 tcaggttggg gtcttttcat tgggtattgg tcctatgatg accacaatga ttttactaag
      301 actatttacg attggtaaat actctagtgg agtatctcaa aaagttcaac agtttcgcca
      361 aaatgtggtg atgcttgtca tagcaattat tcaagggcta gccataacca tatcttttca
      421 atatcataat ggtttttctc tgaccaaatt attactcgcg acaatgatac ttgtgactgg
      481 tgcatatatt atttcctgga ttggtaatct aaatgcggaa tacgggtttg gtggaatgac
      541 tattttagtg gtggtaggta tgcttgttgg gcaatttaat aatattcctc ttatttttga
      601 attattccaa gatggttatc aacttgctat catcttattt cttttgtgga cactagtagc
      661 aatgtactta atgataactt ttgaacgttc agaatatcgt ataccagtaa tgcgtacttc
      721 aatccataat cgtctcgttg atgatgccta catgccaata aaagtaaatg cttcgggggg
      781 gatggcattt atgtatgttt ataccttact aatgttcccc cagtatatta ttattttact
      841 aagaagtatt ttcccaacta atccagatat cacatcatac aatgactatt ttagtttatc
      901 ttctattcag ggagtggtta tttatatgat tttaatgctt gttttatcag ttgcatttac
      961 ttttgtgaat atcgatccga ctaaaatttc tgaggcaatg cgagaaagtg gtgattttat
     1021 tcctaattat agacctggta aagaaacaca aagttattta tcgaaaatct gttatttatt
     1081 tggaactttt agtggttttt tcatggcttt cttaggaggg gtaccattat tatttgctct
     1141 tggaaatgat gatcttagga cagtttcatc tatgacaggg atttttatga tgatcacagg
     1201 gatgtcattt atgatattgg atgaatt
//
LOCUS       DATRSD010000165         1132 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRSD010000165 DATRSD010000000
VERSION     DATRSD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.167"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..810
                     /locus_tag="VBD85_002000"
     CDS             <1..810
                     /locus_tag="VBD85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7134520.1"
                     /translation="KKLAQEDHRIIYLRKENGGVSSARNLGLQYATGSYIGFVDSDDY
                     ISKTMYENLLKRLLETNADIAETDFALIDNRFTKKKRKKIQKVLNKEEAIREFLSGNV
                     VENNLVIKLFKKTVIANLKFKEDVIVGEDMLFSLQALQNSDRVTVDTTNADYFYVVRS
                     NSTMNTITEKDIDNLSILEQEFKKIDIPQLKSYLEAKLIREKVKFVSRVLISNKAHLY
                     SDIIDRYLQEVRHYSIKNMINFLSSKHCLTITIMKISPKLYTLLYKVFQKQ"
     gene            832..>1132
                     /locus_tag="VBD85_002001"
     CDS             832..>1132
                     /locus_tag="VBD85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7134521.1"
                     /translation="MEKISIIIPVYNVKKYLNDCIQSVINQTYNNLEIILIDDGSTDG
                     SGDYCDEIAKKDSRIFVYHKTNGGLSEARNVGIKISTGKYITFIDSDDYIENLYIE"
BASE COUNT          440 a          135 c          199 g          358 t
ORIGIN      
        1 aaaaaattag ctcaagagga tcatcgtatt atttatttaa ggaaagaaaa tggtggggtt
       61 tctagtgctc gaaatttagg gctacaatat gcaacaggaa gttacattgg ttttgtagac
      121 tcagatgact atatatcaaa aacaatgtat gaaaacttat taaagagatt attagaaact
      181 aatgcggata tcgccgaaac tgactttgcc ttaattgata atagatttac gaaaaaaaag
      241 aggaaaaaga tacagaaagt tctgaataaa gaagaagcta ttagggagtt tttatcagga
      301 aatgtggttg aaaataatct cgtcataaag ttatttaaaa aaacagttat tgctaatcta
      361 aaatttaaag aagatgtgat agttggtgaa gatatgctat tttctttgca ggccttacaa
      421 aactcagata gagttacagt ggacactacg aatgcggatt atttttatgt cgtacgttca
      481 aattcaacta tgaatactat taccgagaag gatatcgata atctttcaat actcgagcag
      541 gagttcaaaa aaattgatat cccccaatta aaaagttatt tggaagccaa attaattcga
      601 gaaaaggtta aatttgtatc tagagtatta attagtaata aagcacattt gtacagtgat
      661 attattgata gatatttgca agaggttaga cactacagca taaaaaatat gataaacttt
      721 ctttcgtcga aacattgttt gactataact attatgaaaa tatcacctaa actatataca
      781 cttttatata aagtttttca aaagcaataa atatcggaac ggaggctttt attggaaaag
      841 attagtatta ttataccggt atacaatgtt aagaagtatc ttaatgattg tattcaatct
      901 gttataaacc agacttacaa taatttagag attattttaa ttgatgatgg ttctaccgat
      961 ggttcaggag actattgtga tgaaattgcc aaaaaagata gtcgaatttt tgtgtatcat
     1021 aagacaaacg gcggtctatc agaagctcga aacgttggaa taaaaataag taccgggaaa
     1081 tatattacat ttatagattc agacgattac atcgaaaatt tatatattga aa
//
LOCUS       DATRSD010000166         1099 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRSD010000166 DATRSD010000000
VERSION     DATRSD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.168"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..1033
                     /locus_tag="VBD85_002002"
     CDS             <1..1033
                     /locus_tag="VBD85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEO7134522.1"
                     /translation="FVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERV
                     ISLVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGH
                     HLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLA
                     VKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRGQIEVREYWVSSDIKWLCQ
                     NHPKWHKLRGIGMTRNTIDKDGQLSQENRYFIFSFKPDVLTFANCVRGHWQIESMHWL
                     LDVVYHEDHHQTLDKRAAFNLNLIRKMCLYFLKVMVFPKKDLSYRRKQRYISVHLEDY
                     LVQLFGERG"
BASE COUNT          347 a          171 c          251 g          330 t
ORIGIN      
        1 cttcgtttgt cagttagctg gcattgaaac ctggaaggag atggaagatt ttattgaaat
       61 gaatgaacca ttgtttgcga cctacgttga tttgagtgaa ggttgtccgt ctcatgatac
      121 cttagagcgt gtgattagtc ttgttaattc agaccgttta aaagagctta aagttcaatt
      181 tgagcaatca ttgacaagct tagatgccgt tcatcaactg atttcagtgg acggtaaaac
      241 gattcgaggc aatcgaggta aaaatcagaa gcctgttcat attgtaacgg cttatgatgg
      301 gggtcatcat cttagtttgg gacaggtagc ggttgaggag aaaagtaatg aaattgttgc
      361 cattcctcag ttattgcgga caattgatat ccgtaaaagc attgtaacga tagacgcaat
      421 gggcacgcag acggctatcg ttgatacgat tataaaaggt aaagcagact attgcttagc
      481 cgtcaaagga aatcaagaaa cactttatga tgatattgct ctttatttta gtgatgtcaa
      541 cttattggaa gaactccaag aaaatgcgca gtattatcag actgttgaaa aatctagggg
      601 acagattgaa gttagagaat actgggtgtc ttccgatatc aaatggttgt gtcaaaacca
      661 tcccaaatgg cataagttac gtggtattgg gatgactcgt aacacgattg ataaggatgg
      721 tcagctgagt caagagaatc gttattttat ctttagcttt aagccggatg tcctcacatt
      781 tgccaattgt gtacgaggtc attggcagat agagagtatg cactggttat tggacgttgt
      841 ttatcatgaa gatcatcatc agacattgga taaaagagcc gcatttaacc taaatcttat
      901 ccgaaaaatg tgcttatatt ttctcaaagt gatggtattt cctaaaaaag acctcagtta
      961 tcgtcgcaaa caacggtata tttctgtcca tttggaagat tatttagtcc aattatttgg
     1021 agaaagaggc taagcagatc attgatttaa aaaggaatct tttcaaaatt gatgtccggg
     1081 cacaaaagga tgaagagaa
//
LOCUS       DATRSD010000167         1084 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRSD010000167 DATRSD010000000
VERSION     DATRSD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.169"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..608
                     /locus_tag="VBD85_002003"
     CDS             <1..608
                     /locus_tag="VBD85_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEO7134523.1"
                     /translation="IKINPVNNRIPVSEIQNLTNWHKGFDLGFPFWEEGEKYRKEGNL
                     KRSILLYDLARSNGYCSPALFESYAMAFHRLKDYENEIDILDEGIEREGNLGRLITRR
                     NNAMKMLIKSRNEQKKIKENESAENILFSEIEILKRKKTRKNARPVLKMDDEMNILEH
                     FESISDAVKKTGINSKSIRDASKGIQKHAGGFVWRYEDEYK"
     gene            complement(800..1084)
                     /locus_tag="VBD85_002004"
                     /pseudo
     CDS             complement(800..1084)
                     /locus_tag="VBD85_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          414 a          142 c          203 g          325 t
ORIGIN      
        1 atataaaaat taatcctgtt aataatcgaa ttcctgtttc agaaatacaa aatttgacta
       61 attggcataa aggatttgat ttgggttttc cattctggga agaaggggaa aaatatagaa
      121 aagaaggaaa tttaaaacga tcaattttgt tatatgattt agctagatct aatggctatt
      181 gttcgcccgc actttttgag tcatatgcaa tggcttttca tagattaaaa gactatgaaa
      241 atgaaattga tatcttagat gaaggaatag agcgtgaggg caatttaggt agattaatta
      301 cacgtagaaa taatgcgatg aaaatgttaa tcaaatcaag aaacgaacaa aagaaaataa
      361 aagaaaatga aagtgctgaa aacatccttt ttagtgaaat agagatttta aaaagaaaaa
      421 aaactagaaa aaatgcacgt ccagtcttga aaatggacga tgaaatgaat atattagaac
      481 attttgagtc tatttcagat gcagtaaaaa aaacaggaat taattcaaaa agtatacgtg
      541 atgcttcaaa aggtattcaa aagcatgcag gtggttttgt ctggagatac gaggacgaat
      601 ataagtagat tcattcacat atataaaagt tttgataagt aattaatgat ttcataataa
      661 tcgtattgaa tgaaataatt tactaaagta gacaagacaa aataataata tcagtgttta
      721 tttttagtca ttattatagc attggtaaaa acctgaaaag gcaacacttt ataaatgttg
      781 tacagaaaag tgttgccttt tcagtcttaa gagctagttc ttccagcgat tgaaaggttt
      841 cttgataaac aaattcaatt ttgaaagcac gatacgtact ttcagctacg gcattgtcat
      901 aaggacaacc agcctgacta agcgaacgtg tgattccaaa ggcttccaat atttcatcaa
      961 ttaactgatt atcaaactct ttgccacgat ctgaatggaa catcttgact ttggtcaggg
     1021 cgtaagggat gctttgtatg gcttgcttaa cgagttcagc ggtcttgtgc caaccaagag
     1081 acag
//
LOCUS       DATRSD010000168         1051 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.170, whole genome shotgun sequence.
ACCESSION   DATRSD010000168 DATRSD010000000
VERSION     DATRSD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.170"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(102..584)
                     /locus_tag="VBD85_002005"
     CDS             complement(102..584)
                     /locus_tag="VBD85_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7134524.1"
                     /translation="MLENDIPMASNILAMKDFGDPHNFSAVSPQLKASSEHLKEIMKK
                     YPNAKINIGGHSLGGMDAQYAVVDITAYGARHTYGSVKVQEGVPLEVLAKWFGHKDTS
                     MLRSIYIYIYWTRQKMSGLKEKNYLVGKLIFDKLKSPINKAFFHFCTMPPTTISYKFL
                     "
     gene            complement(578..856)
                     /locus_tag="VBD85_002006"
     CDS             complement(578..856)
                     /locus_tag="VBD85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134525.1"
                     /translation="MKKYIDEEKLQIAMSEYKTIHPNARLKDQYDNTIGVVSEVYDNT
                     SHSGEQVYAVVDNRHIPKEDVKEVTVLFRGSTSPQEFINNLADVTLDC"
     gene            complement(853..>1051)
                     /locus_tag="VBD85_002007"
     CDS             complement(853..>1051)
                     /locus_tag="VBD85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO7134526.1"
                     /translation="IHNYKINDSELNYNPMGGMEVTVYVNNDTNKYFQFTILDNGNGK
                     LESGSYIISADLDNDLETNKK"
BASE COUNT          329 a          196 c          134 g          392 t
ORIGIN      
        1 gtcttgtccc ctttttgtcc cacattattt ttctaacaat aatttttcaa gaatttagca
       61 aaataaaaag cctttaaaat cagcattctt gactattttt atcacaaaaa tttgtaggaa
      121 atcgttgtag ggggcatagt acaaaaatga aaaaaagcct tatttatagg gctttttagc
      181 ttgtcaaaaa tcagtttgcc caccagatag tttttctctt tcaaaccact cattttttgt
      241 ctcgtccaat aaatgtatat atatatactt cttaacatag acgtatcttt gtgaccaaac
      301 cattttgcta atacttctaa aggtactcct tcttggactt taacactacc gtaagtatgt
      361 cttgctccgt aagctgtaat atctacaaca gcatattgag catccatacc tcctaatgaa
      421 tgtccaccaa tgtttatttt tgcatttgga tattttttca taatctcttt taaatgttct
      481 gatgaagctt tcaactgtgg tgatacagca ctgaaattat gaggatcccc aaaatctttc
      541 attgccaaaa tattactcgc cataggaatg tcattttcta acaatccaaa gtcacatctg
      601 caagattatt aataaattcc tgaggagatg ttgatccacg aaaaagtacc gtcacctctt
      661 ttacatcttc ttttggaata tgacgattat caaccacagc ataaacctgt tctccagaat
      721 gagaagtatt gtcgtaaacc tccgaaacaa ctccaattgt gttatcatat tgatccttta
      781 atctggcgtt gggatgaatt gttttatatt ctgacattgc gatttgtaat ttttcttcat
      841 caatatattt tttcattttt tattggtttc taaatcatta tctaaatctg cacttataat
      901 atagctccca gattcaagtt taccatttcc attatctaaa atagtaaatt gaaaatattt
      961 attggtatca ttattcacat aaacagtaac ctccattcct cccataggat tataattgag
     1021 ttctgaatcg ttaattttat aattatgaat a
//
LOCUS       DATRSD010000169         1022 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRSD010000169 DATRSD010000000
VERSION     DATRSD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.171"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(271..393)
                     /locus_tag="VBD85_002008"
     CDS             complement(271..393)
                     /locus_tag="VBD85_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO7134527.1"
                     /translation="MFKGFKKLIIIMGGISLMMILTACDLVKKITNGFKNIENT"
     gene            complement(480..743)
                     /locus_tag="VBD85_002009"
     CDS             complement(480..743)
                     /locus_tag="VBD85_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134528.1"
                     /translation="MELLLKLTGNSESLVLQIYDIDTKGWEERISLEKVEEGFFVKSK
                     IANLEYHTNNNITSNERVLAWSDKEPTNNMNLKILSLTLICIP"
     gene            complement(709..972)
                     /locus_tag="VBD85_002010"
                     /pseudo
     CDS             complement(709..972)
                     /locus_tag="VBD85_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          303 a          201 c          129 g          389 t
ORIGIN      
        1 gtgtaggtga ggtctaagta aagaatttaa gcgttttaat aataaaaaag tgagaacaat
       61 agattctcac tcacaattag cctggtaatt tctcacacga tttggtactg cttttttaaa
      121 attggacgat ggtgttattt ttaatatgag tagctgcact cgaatccgat tgctcaaaag
      181 cacttgtcgc tccattgaga tagctattca gatcagaaat aactggttga atgttttggc
      241 tgacttcttt gataactttt gatccagctt ctaagtattt tcaatgttct tgaacccatt
      301 ggtgatcttt tttactaagt cacatgctgt taaaatcatc attaagctta tccctcccat
      361 aataataatc aattttttaa atcccttaaa catcactttt cctcttatct tattgatttt
      421 ttcaatttta ttgctgtctt gacattgcta tcgcaaacaa ccaactcaat gcaatatagt
      481 tacggtatac atatcaatgt aagactcaag atttttaaat tcatattatt tgtaggttct
      541 ttatcagacc aagctaatac tctttcattt gaagtaatat tattatttgt atgatattca
      601 agattagcta tttttgattt cacaaaaaaa ccttcttcta ctttttctag actgattctt
      661 tcttcccacc ccttcgtatc aatatcatat atttgaagaa ctaaactctc agagtttcca
      721 gtcaatttta gtaataattc caaaaccaat ccttcctaca aaccaacgga tagccctatt
      781 ccaaccggtc ctaattttaa tttcacttgt tacaccaact tttgctatcg cggacaatcc
      841 tattcccgct gatgcactcg catcaatatt taaaccaaat actttaaatc cagcttttgc
      901 ttcttttctt acttccatcc cagtcttgat tgtttttgtc caatcaagac ctttaactga
      961 ggtatcaaat gtattttccc tttgttattg aataatgggt aacgtgtaat aatcattatc
     1021 tt
//
LOCUS       DATRSD010000170         1016 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRSD010000170 DATRSD010000000
VERSION     DATRSD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.172"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            487..777
                     /locus_tag="VBD85_002011"
     CDS             487..777
                     /locus_tag="VBD85_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO7134529.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
BASE COUNT          396 a          138 c          150 g          332 t
ORIGIN      
        1 aatttattta attatctcat atacataaaa aaagtcaata aaaaaatccc aattgattga
       61 gatagctttc gtttatttta taagctccga aactacagaa aattgatacc tttttgatac
      121 cgtttaggtt taaatatttg tctataaata agaatttcag tgatttttta ttttagaaac
      181 gttgatttta aaggaatttg actttatata attaaatgaa ccaataacca ggtcttaaga
      241 aagctcgtaa agcttcacaa ttctcaaaat gttaatcagt acgattatat caacgtttca
      301 aagcactttg caaatcttgc aaggtgtttt ttaaaacctt ttgataaccc tttctgtttt
      361 gaaagtaagg taaatgtaat ataaatcgct gtttgtattt ttaatagatt aacctattat
      421 gagttttatc tgtgtaagga acatctaaat atgctatact gcagttaaaa aaatagggag
      481 caaatgatgt cacaaattaa actaacacct gaagaactcc gtatttcagc acaaaaatat
      541 acaacaggat cacaatcaat tacagatgtg ttaacagttt tgactcaaga acaagctgtt
      601 attgatgaaa attgggatgg tacagcattt gatagctttg aagcccaatt caatgaatta
      661 tctccaaaaa tcacacaatt tgcacaatta ttagaagata taaatcaaca attattgaaa
      721 gttgcggatg ttgtcgaaca aacagactca gatattgcct cacaaattaa taaataattg
      781 gcttattcaa attcataagt ttaacttagg aaggcataag ctctcctaag ttgatatatt
      841 atagctagat gatgtaattg tttcataaag gagtaaaaat gaaaaagagt acccaaataa
      901 tactactaat agttgcatta ttcatacttg tttttagcgg aggattttat atgaaagaac
      961 aacaaagaaa agaagaacta aaacggaatc gagaatatga agttagtcta gtcaaa
//
LOCUS       DATRSD010000171          928 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRSD010000171 DATRSD010000000
VERSION     DATRSD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.174"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            445..>928
                     /locus_tag="VBD85_002012"
     CDS             445..>928
                     /locus_tag="VBD85_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEO7134530.1"
                     /translation="MAYFRKRDNGWEYRISYKDITGKYRQKSKSGFKTKKQAELEANK
                     IEIELSKGFQINNNIEFCTYFKNRSELYKKPNVTATTWDKYNYTLSKIKGYFDKAKLS
                     SIKPSHYQKFISDYLNQYNWQTIKMINTHIRQSIKMAIHEGYITKDFTAFVVLPKPDD
                     S"
BASE COUNT          377 a          140 c          147 g          264 t
ORIGIN      
        1 tattaaataa tataagagag actaggaatg tttgtgccca taacaatagg ctaataggat
       61 ttgaatgtag agctagctct acatactatt ctgacttaca cagtctgttt aatataacac
      121 ctgacgattc aagaaaaaat gtattcacta catttattag tttacaatgc tttcttagta
      181 actcagaata tgcaaaattg cataactcaa taagaaaaag agtaaatcaa ttaaaaaata
      241 aagttgggca agataaagtt aatgaaattc ttgaacaact tggattccca ataaattgga
      301 atgaccaacc aaaacttaga caataaaaaa agccccacgc tcaaattttg gtcaaggaga
      361 gcgtgaggca aaactgtata agaaataagc attaaatggc tcgttttctt gtacctattt
      421 tagcaaattt agaaaggttt agcaatggcg tatttccgta aacgcgacaa cgggtgggag
      481 tatcgaatat cgtataaaga tataactggt aagtaccgac aaaaatcaaa gagcggtttt
      541 aaaacaaaga aacaagctga acttgaagca aataagatag agatagaatt atctaaagga
      601 tttcaaataa ataataatat agaattttgt acttacttta aaaatcggtc tgaactttat
      661 aaaaagccaa atgtaactgc tactacttgg gataagtaca actatacttt gtctaagata
      721 aaagggtatt tcgataaagc caagttatca tcaattaaac caagtcatta ccaaaaattt
      781 attagtgatt atttaaacca gtacaactgg caaactatta aaatgataaa tactcacatc
      841 agacaatcaa taaaaatggc tatccatgaa ggttatatta ctaaagattt cactgctttc
      901 gtcgtccttc cgaaaccaga tgatagta
//
LOCUS       DATRSD010000172          893 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRSD010000172 DATRSD010000000
VERSION     DATRSD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.175"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            275..568
                     /locus_tag="VBD85_002013"
     CDS             275..568
                     /locus_tag="VBD85_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134531.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGSQTHPYEILKVDEDWVQIRRNLKRSKEEVRIVRIHDIKELRPVNK"
     gene            613..828
                     /locus_tag="VBD85_002014"
     CDS             613..828
                     /locus_tag="VBD85_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7134532.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSMEE"
     gene            828..>893
                     /locus_tag="VBD85_002015"
     CDS             828..>893
                     /locus_tag="VBD85_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 domain-containing protein"
                     /protein_id="HEO7134533.1"
                     /translation="MMKNFIKQSDTTFVRIIKSLLI"
BASE COUNT          324 a          103 c          188 g          278 t
ORIGIN      
        1 aacaatcaaa ttaatcattg tttcaaaagc ataatggttt taggagttac tcaaatgaaa
       61 aaggaattta ttattacagg tcggatggtt gcaagagtta tggaacccta gtttgcttat
      121 gtatggtaag taatcttgtt cttgatatgc ttgatataat agaaataaat gaaattgtat
      181 tttgtgtgtt ggtagcatca attgcagggc ttgagttttg tttgcaaaaa gaaagggagt
      241 agatagatgg aattgtttgg cttaatggct ttttatggtc atgacgtata ttggtgtacc
      301 taaagagcaa aaggcactca gtcgtagggg taaacaatta gaaaaaataa atcagggagg
      361 aaaaacactt atgtcacaaa tgattcaaga tttagtagga agcacatgta tgatttattt
      421 agaggatggt tctcaaactc atccttatga gattttaaag gtagatgagg attgggtaca
      481 gattcgtcgc aacttgaaac gaagtaaaga ggaggtgcgt attgtccgta ttcatgatat
      541 caaggaattg cgtccagtca ataaatagag cgaggaataa atctatatat cattttataa
      601 aggagaaaaa tgatgggatt aattttaaaa aatagactca aagaattgcg tgcacgagat
      661 ggtatcaatc aaacagaact cgctaaatta acaggagtct caagacaaac aattagccta
      721 attgaacgcg gagaatatac gccatctgtt attattgcta tgaagatttc tcaagtattt
      781 aaagaatccc tagagaatgt ttttagttta gtgtcaatgg aggaataatg atgaaaaatt
      841 ttataaaaca aagtgatact acatttgttc gaattatcaa aagtctttta att
//
LOCUS       DATRSD010000173          858 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.176, whole genome shotgun sequence.
ACCESSION   DATRSD010000173 DATRSD010000000
VERSION     DATRSD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.176"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            41..721
                     /locus_tag="VBD85_002016"
     CDS             41..721
                     /locus_tag="VBD85_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7134534.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYISFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKVLGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFEKWLDDSNFAVNGYQIRSL
                     SSIEQKTK"
BASE COUNT          312 a          105 c          160 g          281 t
ORIGIN      
        1 tgagatgtag agaattagat ataatctttt taatcagcca gtgggtgatg tcgttgaaaa
       61 ttttacggaa ggtaaaaatc cgaagattga tacacttaat gggaaaacag ttagaataga
      121 aaaaataaat ccagatcatt ttgaagactt atttcaagtt tatggagaat tgtctactga
      181 agatagttta acttatatat cttttagcaa atttaatagt aagaatgaat ttgatgtctt
      241 tttccaaact ctattaaaat cagaagatcc ttattattta gctattgttg ataataatac
      301 tggtaaagta ttagggactt tttcattaat gcgtattgat acaaaaaata gagttgttga
      361 aatgggatgg gtggtttatt catcaaaact aaaacaaaca aggatagcaa cagaagctca
      421 atatttggtt atgaaatatg tttttgaaga actctgttac cgccgttatg agtggaaatg
      481 tgatagtttg aatgcaccat ctaataattc tgcaaaacga cttggtttta cttttgaagg
      541 aacttttaga caagctgtcg tatataaggg tcgtaacaga gatacaaatt ggtattctat
      601 tttagataaa gagtggccag agaaaaaaac acggtttgaa aaatggctag atgattcaaa
      661 ctttgctgta aatgggtatc aaatcagatc actatcaagc attgagcaga agacaaaata
      721 gagtcattca ttaatcacta aaatttcaaa attacatgtt tttttagcta acatcttaaa
      781 ctttgttaaa taaatgttta ttaactttta aaggtgcatg gtaggactca aaatcgggtg
      841 tcctcacgga cgtgccag
//
LOCUS       DATRSD010000174          821 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRSD010000174 DATRSD010000000
VERSION     DATRSD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.177"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(151..234)
                     /locus_tag="VBD85_002017"
     tRNA            complement(151..234)
                     /locus_tag="VBD85_002017"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Leu,seq:caa)
     gene            complement(245..316)
                     /locus_tag="VBD85_002018"
     tRNA            complement(245..316)
                     /locus_tag="VBD85_002018"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(282..284),aa:Gln,seq:ttg)
     gene            complement(323..395)
                     /locus_tag="VBD85_002019"
     tRNA            complement(323..395)
                     /locus_tag="VBD85_002019"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:His,seq:gtg)
     gene            complement(410..480)
                     /locus_tag="VBD85_002020"
     tRNA            complement(410..480)
                     /locus_tag="VBD85_002020"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Trp,seq:cca)
     gene            complement(487..567)
                     /locus_tag="VBD85_002021"
     tRNA            complement(487..567)
                     /locus_tag="VBD85_002021"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Tyr,seq:gta)
     gene            complement(579..651)
                     /locus_tag="VBD85_002022"
     tRNA            complement(579..651)
                     /locus_tag="VBD85_002022"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Phe,seq:gaa)
     gene            complement(654..727)
                     /locus_tag="VBD85_002023"
     tRNA            complement(654..727)
                     /locus_tag="VBD85_002023"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Met,seq:cat)
BASE COUNT          231 a          207 c          187 g          196 t
ORIGIN      
        1 taagaatctt tttcaggatc atgataatat tgccaaatat cttattgaaa aaaatctaaa
       61 catgaactgg taacattttg gttacttata aagttaaaat atttaaaata caaaaaagac
      121 aagggtctag aaaccttgtc ttttcaagta taccggcggc cggggtcgaa ccggcacgtc
      181 cgtgaggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc cggctatctt
      241 aaaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
      301 gcttggctat accccatgaa aaggcgagtg atgggaatcg aacccacgaa tgtcagagcc
      361 acaatctgat gtgttaacca cttcaccaca cccgccatat tagaaaaaac acgggcagta
      421 ggaatcgaac ccacactgaa ggttttggag accttagttc tacctttaaa ctatgcccgt
      481 ttactatgga gagagaggga ttcgaacccc cgaacccgaa ggagcggatt tacagtccgc
      541 cgcgtttagc ctcttcgcta tctctcctaa ggtataaatg gcgcgagacg gaatcgaacc
      601 gccgacacat ggagcttcaa tccattgctc taccaactga gctaccgagc ctattgcggg
      661 agcaggattt gaacctacga ccttcgggtt atgagcccga cgagctacct agctgctcca
      721 tcccgcgata tctttaaagg aggatgtggg attcgaaccc acgcacgctt ttacacgcct
      781 gacggttttc aagaccgttc ccttcagccg gacttgggta a
//
LOCUS       DATRSD010000175          817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.178, whole genome shotgun sequence.
ACCESSION   DATRSD010000175 DATRSD010000000
VERSION     DATRSD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.178"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            17..>817
                     /locus_tag="VBD85_002024"
     CDS             join(17..391,394..>817)
                     /locus_tag="VBD85_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEO7134535.1"
                     /translation="MNYEASKQLTDVRFKRLVGVQRTTFEEMLAVLKTAYQRKHAKGG
                     RTPKLSLEDLLMATLQYMREYRTYEQIAADFGIHESNLIRRSQWVESTLIQSGFTISK
                     THLSAEDTVIVDATEVKINRPKKQLANYSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAAKILADSGYQGIMKMYSQAQTPRKSSKLKPLTLEDKTYNHT
                     LSKERIKVENIFAKVKTFKIFSTTYRNRRKRFGLRMNLIAGMINRELG"
BASE COUNT          286 a          147 c          167 g          217 t
ORIGIN      
        1 atggtacaat agttctatga attatgaagc aagcaaacaa ttaactgatg tacgatttaa
       61 gcgccttgtt ggtgttcagc gcactacttt tgaagagatg ttagctgtgt taaaaacagc
      121 ttatcaacgt aaacacgcaa aaggtggccg aacccctaag ttaagcttag aagatctcct
      181 catggctact cttcaataca tgcgagaata ccgcacttat gaacaaattg cggctgattt
      241 tggcattcac gaaagcaact taatccgtcg aagtcaatgg gttgaatcaa ctcttattca
      301 aagtggtttt acgatttcaa aaactcatct tagtgctgag gatacggtga ttgtggatgc
      361 aacagaggta aaaatcaatc gtcctaaaaa aatcaactag cgaattattc tggtaaaaag
      421 aaatgtcatg ctatgaaggc tcaggcgatt gtcacaagcc aagggagaat tgtttctttg
      481 gatattgcag tgaactattg ccacgatatg aagttgttca aaatgagtcg cagaaacatc
      541 ggacaagctg ctaaaatctt ggcagacagt ggttatcaag ggatcatgaa gatgtattca
      601 caagcgcaaa ctccgaggaa atcaagcaaa cttaagccac taactcttga agataaaacc
      661 tataaccata cgctatccaa agagagaatc aaggttgaga atatttttgc caaagtaaaa
      721 acgtttaaaa tattttcaac aacctatcga aatcgacgca aacggtttgg attacgaatg
      781 aatttgattg ctggaatgat caaccgtgaa ctaggat
//
LOCUS       DATRSD010000176          749 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRSD010000176 DATRSD010000000
VERSION     DATRSD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.181"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..>749
                     /locus_tag="VBD85_002025"
     CDS             <1..>749
                     /locus_tag="VBD85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEO7134536.1"
                     /translation="EQLDELLQVSTFMEKMGVKKEIDLSLFDNQSQRNLYFLNKGLVL
                     GEKVSSNYDESKLLHLRVANANFLTFYQFEKTNSGRLINIFLETPWCKKGDSIVDENA
                     EDVSIFEVFEPNDWLKIDNCDFDAVIASYQRLVDTGNFVSSADETLIRIISASDLSED
                     VGRKNLLLDWAQQLSNWNTTHSKGYERALINDLQIKIRRRPLNDKEKEKLNEILTSNI
                     EDSEVCFGVTVLLKSKSQADYYWNKLEEELQ"
BASE COUNT          273 a           89 c          145 g          242 t
ORIGIN      
        1 gtgagcagtt agatgaactt cttcaggttt caacttttat ggaaaaaatg ggagtgaaaa
       61 aggaaataga tttgtcactt tttgataacc agtcacagag aaatttatat tttcttaata
      121 aaggtcttgt tttaggagaa aaagtttctt caaattatga cgaatcaaaa ttattacatc
      181 taagagttgc taatgcaaat tttctcactt tctatcaatt tgaaaaaact aactcaggta
      241 gactaattaa tatatttttg gaaacacctt ggtgtaaaaa aggggatagt atagttgatg
      301 agaatgcaga agatgtttcg atttttgagg tttttgagcc aaatgattgg ttaaagatag
      361 ataattgtga ttttgatgca gtaattgcct catatcagag attagttgac acaggaaact
      421 ttgtttcaag tgccgatgaa acccttatta gaattatttc tgcttcagat ttatcagaag
      481 atgtgggaag aaagaattta cttcttgatt gggctcagca gctttcaaat tggaatacga
      541 cacattccaa aggctatgaa agagcattga taaatgattt acaaattaaa atcagaagac
      601 gtcctctgaa tgataaagaa aaagagaaat taaatgaaat attgactagc aatattgaag
      661 attctgaagt ttgttttggt gtgacggtcc tactgaaatc aaaatctcaa gcagattatt
      721 attggaacaa attagaggaa gaattacag
//
LOCUS       DATRSD010000177          721 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.183, whole genome shotgun sequence.
ACCESSION   DATRSD010000177 DATRSD010000000
VERSION     DATRSD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.183"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..675)
                     /locus_tag="VBD85_002026"
     CDS             complement(<1..675)
                     /locus_tag="VBD85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO7134537.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKLGKIDPLQLIVLEQG
                     FTAKYVFRQGTKYYGDVSQLQSTGRASLTYNIFGEDGLPHVKTDGQIDIVSVALTIYD
                     STTLRDKIEEVRTNANDPKWTEESRTEVLTGLDTIKTDIDNNPKTQTDIDSKIVEVNE
                     LEKLLVL"
BASE COUNT          225 a          147 c           86 g          263 t
ORIGIN      
        1 caatactaac aatttctcta attcattaac ctcaacaatt ttactatcaa tatctgtttg
       61 cgtcttagga ttattatcaa tatctgtctt aattgtatct aatcctgtta aaacctcagt
      121 acgactttct tccgtccact taggatcgtt tgcattcgtt ctaacttctt caatcttatc
      181 cctcaaggtt gttgaatcat aaatagttaa agcaacacta actatatcaa tttgtccatc
      241 agtctttaca tgtggtagtc catcttcacc aaatatatta taggtaagac tagcccttcc
      301 tgtactctgc aactgagaaa catccccata gtatttagta ccttgtctaa aaacatactt
      361 tgctgtaaaa ccttgttcta aaacaattaa ttgtaatgga tctattttac ctaattttac
      421 atcatataaa tctatgtaag catttccgtt ttgtatattt ttagtgatgc ttgtatttaa
      481 ggtcgctgca ctccctggaa ttgtagatgc agcaactatt gactcttcaa aaatattaag
      541 attaccttgt gtaaccccac ccaaaaaact aagaccaatt agtacagaag ctgcaccaaa
      601 cttgaacttc ttaattgaaa accgttgttt cgtctgtgaa gtatcataac tgttattttt
      661 agaccttcta aacataatat tctcctttac tctgtataac tctatacaga gtaaattata
      721 g
//
LOCUS       DATRSD010000178          718 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.184, whole genome shotgun sequence.
ACCESSION   DATRSD010000178 DATRSD010000000
VERSION     DATRSD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.184"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            1..249
                     /locus_tag="VBD85_002027"
     CDS             1..249
                     /locus_tag="VBD85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO7134538.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(333..>718)
                     /locus_tag="VBD85_002028"
     CDS             complement(333..>718)
                     /locus_tag="VBD85_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7134539.1"
                     /translation="KLGAQSGSSGFDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLK
                     NNRIDGLLIDEVYANYYLKQEGNIKAYYFVKTAYQGENFVVGARKVDRRLIEKINKAF
                     KQLHNKGRFQKISYKWFGEDVYSKE"
BASE COUNT          250 a          137 c           97 g          234 t
ORIGIN      
        1 atggaggtga aaacattggc aaatattaaa tcagctatca aacgcgctga acttaacgtt
       61 aaacaaaacg aaaaaaactc agcacaaaaa tcagctatgc gtactgctat taaagcattt
      121 gaagctaacc cttcagaaga actttaccgc gctgcttctt caagcatcga caaagctgct
      181 tctaaaggtt taattcacac aaacaaagca agccgcgata aagcacgtct tgcaactaaa
      241 cttggttaat taacctagaa aacatcggat atatcgatgt tttttattta attttttcgt
      301 agaataaaag actaggagaa attcctagtc ttttattctt tactataaac atcttcacca
      361 aaccatttgt aagagatttt ttgaaatctc cccttattat gaagctgttt gaaagctttg
      421 ttaatctttt caattagtct acgatcaact ttacgagctc ctactacaaa attttctcct
      481 tgataagcag ttttaacaaa ataataagct tttatatttc cttcttgctt taaataatag
      541 ttagcataaa cttcatcaat caaaagacca tcaatacggt tattttttaa atcaatcaaa
      601 gcctgagtga aagtatcgta ttgaactgct tcttttcctt ttacaaactt ttttaaaata
      661 tcaggtttag cgttaaaagc atcaaaacca gatgaacccg actgggctcc tagttttt
//
LOCUS       DATRSD010000179          661 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.185, whole genome shotgun sequence.
ACCESSION   DATRSD010000179 DATRSD010000000
VERSION     DATRSD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.185"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..>661
                     /locus_tag="VBD85_002029"
     CDS             <1..>661
                     /locus_tag="VBD85_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7134540.1"
                     /translation="NHFEKTINPLDKKHHAFYVSTAVTLLHKASLRLGFEYTNSDIEY
                     LSQMLLNYRFEPIPVQQNDDKFVTALLEDVSKAMSINFLEDQYLFDQLSQHIPAMLYR
                     LKYHTHIQNPFTDKIKLEFAITYNVLWLVLQDLGQELDVVFNEDEVAYLTIYFQLSIE
                     KIGKSRKILIVCQTGIVTSELLINRIKKVVPSLDQVEIASLSEMYEKDLTCYDFVLTT
                     IE"
BASE COUNT          230 a           94 c          111 g          226 t
ORIGIN      
        1 aatcattttg aaaagactat aaaccctttg gataaaaagc atcatgcctt ttatgtgagt
       61 acagctgtaa cattattaca taaagcttcc ttacgattag gttttgaata tacaaatagt
      121 gatattgagt acctttctca aatgttattg aattataggt ttgaaccgat accagttcag
      181 caaaatgatg ataaatttgt cactgcccta ttggaggatg tttcaaaggc tatgtccatc
      241 aattttttgg aagatcaata tctttttgat caacttagtc aacatatacc agccatgttg
      301 taccgattga aataccatac gcatatacaa aatccattta cagataaaat aaaattggag
      361 tttgccataa cgtataatgt actttggttg gtgttacagg acttaggtca agagttagat
      421 gttgttttta atgaagatga agtagcctac ttaaccattt attttcaact ttccattgaa
      481 aaaattggga aaagtagaaa aatcttaata gtatgtcaaa ctgggattgt gacttctgaa
      541 ttactaatta atagaataaa aaaagttgtg ccttcactag atcaagttga aattgcatcc
      601 ttaagtgaga tgtatgagaa agatttgact tgttatgact ttgtccttac aactattgaa
      661 a
//
LOCUS       DATRSD010000180          655 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.186, whole genome shotgun sequence.
ACCESSION   DATRSD010000180 DATRSD010000000
VERSION     DATRSD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.186"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(32..589)
                     /gene="lepB"
                     /locus_tag="VBD85_002030"
     CDS             complement(32..589)
                     /gene="lepB"
                     /locus_tag="VBD85_002030"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7134541.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
BASE COUNT          234 a          118 c           76 g          227 t
ORIGIN      
        1 aagaatggta tcaaataata ccattctttt actactctga agctgtaaaa ccaaattcac
       61 ttagagggta tactttaaag gtaataacac cacgaatatc tttctctttg attaatccaa
      121 attttcgact atcattttta ttttgtcgat tatcatttaa cacaagataa gaacccttag
      181 gaactctgct ttctttattt cgtgttaatg tttcaactga gaagtcggaa gtaaagggca
      241 tttgaccgtc tttcttttca gaataagcat ttttcatttt ttcaatgtaa ggctcatcct
      301 tgaaaacatc attcaaatat aaaatatcat ccattactct cactttttga tttggctcac
      361 caataacacg actaatatag aaaatctttc caaccttata gacaataaag tctttgtact
      421 taggcgtcct attacgatta acaacaacaa catctccgtt gctaaaataa gaatttgtag
      481 catccttatg tactttaaag gttgcaaaaa caaaatatct caagagaatc aaaatcaata
      541 cagcaatcaa tgctaaaata atatttctaa taaaatcgcg tttaaccata actactccta
      601 atcttttttc cattatatca tgttttcata gataaaaaaa ggagggtaat tatta
//
LOCUS       DATRSD010000181          633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.188, whole genome shotgun sequence.
ACCESSION   DATRSD010000181 DATRSD010000000
VERSION     DATRSD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.188"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(1..73)
                     /locus_tag="VBD85_002031"
     tRNA            complement(1..73)
                     /locus_tag="VBD85_002031"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="VBD85_002032"
     tRNA            complement(93..166)
                     /locus_tag="VBD85_002032"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="VBD85_002033"
     tRNA            complement(182..255)
                     /locus_tag="VBD85_002033"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VBD85_002034"
     tRNA            complement(263..336)
                     /locus_tag="VBD85_002034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="VBD85_002035"
     tRNA            complement(348..432)
                     /locus_tag="VBD85_002035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="VBD85_002036"
     tRNA            complement(441..512)
                     /locus_tag="VBD85_002036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
     gene            complement(546..618)
                     /locus_tag="VBD85_002037"
     tRNA            complement(546..618)
                     /locus_tag="VBD85_002037"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Thr,seq:tgt)
BASE COUNT          177 a          179 c          141 g          136 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 tgtaatgccg gctacatgac ttgaacacgc gaccctctga ttacaaatca gatgctctac
      601 caactgagct aagccggcaa tctactaatg cgg
//
LOCUS       DATRSD010000182          535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.190, whole genome shotgun sequence.
ACCESSION   DATRSD010000182 DATRSD010000000
VERSION     DATRSD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.190"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            57..>535
                     /locus_tag="VBD85_002038"
     CDS             57..>535
                     /locus_tag="VBD85_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO7134542.1"
                     /translation="MNEVLRQEKKYLISLEDYYLLSKKFSQVMTLDSHSSDDGYTIRS
                     LYFDSLEDVDWQEKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKT
                     DAQALINGNNSVLLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITF"
BASE COUNT          200 a           75 c           96 g          164 t
ORIGIN      
        1 taatttattg ttaatattta tatataaatt aatatttaat taaacatagg tgagtgatga
       61 acgaagttct tagacaggaa aaaaaatatt taatatcact tgaagattac tatcttttat
      121 ctaagaaatt ttcccaagta atgacgctcg attcgcatag ttcagacgac ggctatacta
      181 tccgttcgct atactttgat tctttagaag atgttgattg gcaagaaaag gaagatggtg
      241 ttgagatacg aagaaagatt cgtttgcgca attatggtag tcaaagcaat tttgcgaaat
      301 tagaaatgaa acaaaaacaa ggtgctaatc aaaaaaagcg ctctctaaaa ttaagcaaga
      361 cagatgctca agcgctgatt aatggtaaca atagtgtttt attaaattat ccggatacat
      421 ttgctgcgga gtgttattca aggataacaa cgcaacttta tcaacctaaa acggttattg
      481 agtaccaaag aaaagctttt gttgctaagg aaaacaatat ccgaatcacc tttga
//
LOCUS       DATRSD010000183          522 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.191, whole genome shotgun sequence.
ACCESSION   DATRSD010000183 DATRSD010000000
VERSION     DATRSD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.191"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..>522
                     /locus_tag="VBD85_002039"
     CDS             <1..>522
                     /locus_tag="VBD85_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO7134543.1"
                     /translation="LKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLP
                     PIPKAIKEGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLM
                     DSKTAIETAKHIQVIKRTLYDNKRDFFELFPVILTDNGGEFARVDDIEIDVCGQSQLF
                     FCDPNRSDQKARIE"
BASE COUNT          165 a          117 c          109 g          131 t
ORIGIN      
        1 ctcaaaacca acgatctaga agtgagttct tcaaccgttt atcgacacat caagaaaggc
       61 tacctatcca tcacaccaat cgacctaccc agagctgtga agttcaaaaa aaggcgaaag
      121 agcacactcc ctcctattcc aaaagcgatt aaagaagggc gacggtacga ggattttata
      181 gaacacatga accaatcaga gctgaactcc tggcttgaaa tggacacggt tattggacgg
      241 attggtggaa aggtccttct caccttcaat gtcgccttct gtaacttcat ctttgccaaa
      301 ctgatggatt ctaagacagc tatcgaaact gctaaacaca tacaggtcat taagaggaca
      361 ctctatgata acaagagaga cttcttcgaa ctctttcctg ttatcctaac ggataatggt
      421 ggggaattcg ctagagtgga tgatattgag atagatgttt gtggacagtc tcaactcttc
      481 ttttgtgacc ctaatcggtc tgaccagaaa gcaagaatcg ag
//
LOCUS       DATRSD010000184          438 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.195, whole genome shotgun sequence.
ACCESSION   DATRSD010000184 DATRSD010000000
VERSION     DATRSD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.195"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(236..>438)
                     /locus_tag="VBD85_002040"
     CDS             complement(236..>438)
                     /locus_tag="VBD85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO7134544.1"
                     /translation="FDNLTQEDINLALSHINSVKRQALNGKTAYELFSFTYGKDIASI
                     LGIEEITAEDVCQSPKLLKDKI"
BASE COUNT          133 a           72 c           90 g          143 t
ORIGIN      
        1 ccacctctag tagaaatgga atttagtctg ataaaaacca ataaaaacca acgaaaatcc
       61 gtctcagact aagttccgtt ggtttttata atacttgatt tcataggttt tcagagttaa
      121 aaatcgcaaa aaaagggtac aagaaaacat cctcatctga aagcgtttca gattaagttc
      181 cgctatggtt gaagttagtg tgagacgttt aggttgtatc agttagttga ttaggttaga
      241 ttttgtcttt taatagtttg ggagattggc agacgtcttc cgcagtaatt tcctcaatac
      301 cgagtatact tgcgatgtct tttccgtaag taaaagagaa tagctcataa gccgttttac
      361 catttagagc ctgtctctta acgctgttga tatgagatag tgccagatta atgtcctctt
      421 gagtcaagtt gtcgaagg
//
LOCUS       DATRSD010000185          412 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.198, whole genome shotgun sequence.
ACCESSION   DATRSD010000185 DATRSD010000000
VERSION     DATRSD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.198"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            complement(<1..>412)
                     /locus_tag="VBD85_002041"
     CDS             complement(<1..>412)
                     /locus_tag="VBD85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134545.1"
                     /translation="TLINLVTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLY
                     YFSNEGSHKLMVYGATWFIGILFISLILFFSVLFRKTLGVLLGCLFMVVLCVVSGFFK
                     ISKSYNPMLLIQKQGEMLSGKFKVQDLMEPSILVF"
BASE COUNT          162 a           72 c           52 g          126 t
ORIGIN      
        1 aaacactaat atgctcggtt ccattaagtc ttgaacttta aactttccac ttaacatttc
       61 tccttgtttt tggataagaa gcataggatt atagctttta ctgattttaa aaaatccaga
      121 tactacacat aacaccacca taaaaagaca acctaacaaa acccctaaag ttttacgaaa
      181 aagaactgaa aaaaagagta ttaagcttat gaaaagaata ccgataaacc atgttgcacc
      241 gtaaaccatt aatttatgac ttccttcgtt actaaagtaa tataacgtat aactaaaatg
      301 gatgacaaca cttaataagt atgacactgt ccatagaagg gtaattacta taaatttagc
      361 aaggattata acttttttgg ctaatccctt cgtaactaga ttaattaagg tt
//
LOCUS       DATRSD010000186          358 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.denovo.201, whole genome shotgun sequence.
ACCESSION   DATRSD010000186 DATRSD010000000
VERSION     DATRSD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.denovo.201"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            <1..>358
                     /locus_tag="VBD85_002042"
     CDS             <1..>358
                     /locus_tag="VBD85_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEO7134546.1"
                     /translation="ENFREENRVRSVSEFVEKTIALSNKENVEHLYYRGESSFYPLRL
                     PTLYRNKRLTLDGSSYYYQQLFIELGKGTYQNSVELSQSIAEFQHYGAITRYLDVTSN
                     PLVALYFAVENEGDNGC"
BASE COUNT          128 a           48 c           67 g          115 t
ORIGIN      
        1 gagaatttta gagaagaaaa tagagtaagg tctgtgtcgg aatttgttga aaaaacaatt
       61 gctttatcaa ataaagaaaa tgttgaacat ttatattata ggggagaaag ttcgttttat
      121 cctttaagac taccaacttt gtatagaaat aaacggctaa ccttagatgg atcctcctat
      181 tattaccaac aacttttcat tgaactcggg aaaggtacat atcaaaattc tgtagagtta
      241 agtcaatcaa ttgctgaatt tcagcattat ggtgcgataa ctagatattt agatgttact
      301 tcaaatccct tagtagccct atattttgct gtagaaaatg agggagataa tggatgca
//
LOCUS       DATRSD010000187        16614 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699508-rid18549223.guided.050, whole genome shotgun sequence.
ACCESSION   DATRSD010000187 DATRSD010000000
VERSION     DATRSD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699508
            Sequence Read Archive: SRR7788390
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789897.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789897.1
            Genome Coverage       :: 145x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:09:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 10 of 43
            Pseudo Genes (internal stop)      :: 15 of 43
            Pseudo Genes (multiple problems)  :: 5 of 43
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699508-rid18549223.guided.050"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2009-09-29"
                     /collected_by="RMPRU"
     gene            287..601
                     /locus_tag="VBD85_002043"
     CDS             287..601
                     /locus_tag="VBD85_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7134547.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            617..1003
                     /locus_tag="VBD85_002044"
     CDS             617..1003
                     /locus_tag="VBD85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7134548.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1032..2417
                     /locus_tag="VBD85_002045"
     CDS             1032..2417
                     /locus_tag="VBD85_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7134549.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2420..2572
                     /locus_tag="VBD85_002046"
     CDS             2420..2572
                     /locus_tag="VBD85_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7134550.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2595..3800
                     /locus_tag="VBD85_002047"
     CDS             2595..3800
                     /locus_tag="VBD85_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7134551.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3843..4064
                     /locus_tag="VBD85_002048"
     CDS             3843..4064
                     /locus_tag="VBD85_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7134552.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4181..4678
                     /locus_tag="VBD85_002049"
     CDS             4181..4678
                     /locus_tag="VBD85_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7134553.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4653..5159
                     /locus_tag="VBD85_002050"
     CDS             4653..5159
                     /locus_tag="VBD85_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7134554.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5143..7590
                     /locus_tag="VBD85_002051"
     CDS             5143..7590
                     /locus_tag="VBD85_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7134555.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7593..9770
                     /locus_tag="VBD85_002052"
     CDS             7593..9770
                     /locus_tag="VBD85_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7134556.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9767..10768
                     /locus_tag="VBD85_002053"
     CDS             9767..10768
                     /locus_tag="VBD85_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO7134557.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10765..11697
                     /locus_tag="VBD85_002054"
     CDS             10765..11697
                     /locus_tag="VBD85_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7134558.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11942..12058
                     /locus_tag="VBD85_002055"
     CDS             11942..12058
                     /locus_tag="VBD85_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7134559.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12074..13993
                     /gene="tet(M)"
                     /locus_tag="VBD85_002056"
     CDS             12074..13993
                     /gene="tet(M)"
                     /locus_tag="VBD85_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7134560.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14112..14279
                     /locus_tag="VBD85_002057"
     CDS             14112..14279
                     /locus_tag="VBD85_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7134561.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14339..14692)
                     /locus_tag="VBD85_002058"
     CDS             complement(14339..14692)
                     /locus_tag="VBD85_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134562.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15197..15619
                     /locus_tag="VBD85_002059"
     CDS             15197..15619
                     /locus_tag="VBD85_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7134563.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15616..15846
                     /locus_tag="VBD85_002060"
     CDS             15616..15846
                     /locus_tag="VBD85_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7134564.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16307..16510
                     /locus_tag="VBD85_002061"
     CDS             16307..16510
                     /locus_tag="VBD85_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7134565.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
BASE COUNT         5376 a         3002 c         3473 g         4763 t
ORIGIN      
        1 ctttatcaag gtcaaaacca ctcaatttac tactaattta ctacttatga atgagctttg
       61 atacgacgat ttatccttga aaagtgaaga tataaagata cttccaataa aatttgaata
      121 tttaataggt agacacttca aaaaatgagg tgtctatttt tttacccgat tttgaaagga
      181 agtgaactta tgaaaacaaa aaatcaagaa tcaaaaggtc gttccccact ctttaagacc
      241 atcaaacatt cattcagcca ataaaaaaga aaggataggt aaaaatatgg aacttaaatt
      301 tgtgattccc aacatggaaa aaacattcgg caatttagaa tttgctggcg aggataaagt
      361 cgttcagcga agaatcaacg gacggctaac tgtcttatca agaagctata atctctattc
      421 tgatgttcaa agagcagatg atattgtggt ggtgcttcct gctgaagctg gcgaaaaaca
      481 tttcggcttt gaggaacgtg tgaagttagt caatccacgt attaccgcag agggctacaa
      541 aatcggcact cgtggtttta caaattacct tttacatgct gacgacatga taaaagaata
      601 aagaaagaga ggaaaaatga tgagattagc aaatggcatt gtattagata aagacacgac
      661 ttttggagaa ttgaaattct ctgctctacg tcgtgaagtg agaatccaaa atgaagacgg
      721 gtcggtttca gatgaaatca aggaacgtac ctatgactta aaatccaaag gacaaggacg
      781 catgattcaa gtaagtattc ctgccagcgt gcctttgaaa gagtttgatt ataacgcacg
      841 ggtggaactt atcaatccca ttgcggacac cgttgctact gccacctatc aaggagcaga
      901 tgttgactgg tatatcaagg cagacgatat tgtgctgaca aaggattcta gttcattcaa
      961 agctcaacca caagcaaaga aagaaccgac acaagacaaa tagtcgctag gtagaaagga
     1021 gactttttcg catgaaacag cgtggtaaaa ggattcgccc atctggtaaa gatttagtct
     1081 ttcattttac gatagcgtca ctcctgcctg ttttcctgct ggttgtcgga ctgtttcatg
     1141 tgaagacaat ccagcagatc aactggcagg attttaacct atcacaagca gataagattg
     1201 acattcccta tttaattatc agtttcagtg tcgcaattct tatctgcttg ctggtagcgt
     1261 ttgtattcaa acgggttcgc tatgatacgg ttaaacaact ttaccaccgt caaaaactgg
     1321 caaagatgat acttgaaaac aagtggtatg aatctgaaca ggtcaaaaca gagggtttct
     1381 ttaaagatag tgctggtcgt acaaaggaaa agataaccta cttccctaaa atgtattatc
     1441 gacttaaaaa tggcttgata cagatacggg tggaaatcac gctgggaaaa tatcaagacc
     1501 aactcttaca cttggaaaag aaattagaga gtggcttgta ctgtgagctg acggataaag
     1561 agttaaagga ttcctatgtg gaatatactt tgctctatga caccatagcc agtcgtattt
     1621 ctattgatga agtagaagct aaagatggta aacttcgctt aatgaaaaac gtatggtggg
     1681 aatatgataa gctccctcat atgttgattg ctggtggtac aggtggcggt aaaacttact
     1741 ttatactgac actgattgaa gccttgcttc atacagattc aaaactgtat attcttgacc
     1801 cgaaaaatgc tgatcttgcg gacttaggtt ctgtgatggc aaatgtctac tatagaaaag
     1861 aagacttgct ttcttgcatt gaaacattct atgaagaaat gatgaaacgt agtgaggaaa
     1921 tgaagcagat gaagaactat aagactggca aaaattatgc ttacttaggt ctcccggcac
     1981 acttcttaat ctttgatgaa tacgtcgctt tcatggaaat gctgggaaca aaagaaaaca
     2041 ccgcagttat gaataagctg aaacagattg tcatgttagg tcgtcaagct ggcttctttc
     2101 taatactggc ttgtcaacgt ccagacgcaa aatatttagg cgacggaatc cgtgatcagt
     2161 ttaatttcag agtggcttta ggtcgtatgt ctgaaatggg ctatggcatg atgtttggca
     2221 gtgacgtaca aaaggatttc ttcttaaagc gaatcaaagg tcgtggctat gttgatgtag
     2281 gaacaagtgt catatcagag ttttatactc cccttgtacc aaaaggatat gatttcttgg
     2341 aggaaattaa aaagttatcc aacagcagac agtccacgca ggcgacgtgc gaagcggaag
     2401 tcgcaggtgt ggactgatct tgctggctgg tgtggcaata gccacgccag cacttaaccc
     2461 cccgtatcta acaggggggt acaaatcgac aggaaacagt caaaaaaaca ttagaaaatc
     2521 ctttggttac aagggattta caaaatttca gcgtatgtca aatgggcttt aaaagttgac
     2581 atacgccttt ttgattggag ggatttttac tgaatgaaca aacttggtta cagcatttaa
     2641 aagaaaaacg cttggcttat ggactatctc aaaaccgttt agctgttgcg actggtatta
     2701 caaggcagta tctaagcgat attgaaacag gaaaagtcaa gccatcagag gatttacagc
     2761 agtccctttg ggaagctctg gaacgcttca atcccgacgc tccccttgaa atgctgtttg
     2821 attatgtaag aattcgcttt ccgacaacag acgtacagca ggtggtcgaa aacatcttac
     2881 aactgaaact gtcctatttt cttcatgagg actatggttt ctattcttat tcagagcatt
     2941 atgctttagg cgacatattc gtcctttgct cccatgaact ggacaaagga gttctggtgg
     3001 aattgaaagg tcgtgggtgc agacaatttg aaagctatct tctggcacaa caaagaagct
     3061 ggtatgagtt ctttatggac gttttggtgg ctggcggtgt gatgaaacgc cttgaccttg
     3121 ccattaacga taagacaggg attttaaata tccctgtact cactgaaaag tgccaacagg
     3181 aagaatgtat ctccgtcttc cgcagtttta aaagctatcg cagtggcgaa ctggtacgca
     3241 aagaggaaaa ggaatgtatg ggaaacaccc tctatatcgg ttcattacaa agtgaagttt
     3301 atttctgtat ctatgaaaag gactacgagc agtacaagaa aaatgatatt cccattgaag
     3361 acgcagaagt aaaaaaccgt tttgagattc gattgaaaaa tgagcgtgcc tattatgcag
     3421 tccgtgattt actcgtctat gacaatccag agcataccgc ctttaaaatt atcaatcggt
     3481 atatccgttt tgtagataaa gacgattcca aacctcgttc tgattggaaa ctgaatgaag
     3541 aatgggcttg gtttattggg aacaatcgtg aacgattaaa actaaccaca aaaccagagc
     3601 cttactcctt ccaaaggacg ctgaactggc tatctcatca agttgccccg accttaaagg
     3661 ttgcgattaa acttgatgaa atcaaccaga cgcaggttgt aaaagacatt ctcgaccatg
     3721 cgaaactgac agaccgacac aagcagattt tgaagcaaca gtcagtaaaa gaacaggacg
     3781 tgataacaac aaaaaaataa ctcaaataca aattcattga atatagagag gagaacattt
     3841 ttatgaattt tggacaaaac ctttataact ggtttctatc aaacgctcaa tcactggtgc
     3901 ttttagcaat cgttgtgatt ggcttgtatc ttggcttcaa gcgtgagttt agcaaactga
     3961 ttggcttttt aattattgcg attattgcgg ttggcttagt cttcaacgct gctggagtaa
     4021 aagacatttt actagagcta ttcaatcgca ttattggtgc ttaaataaaa ccgttctttt
     4081 gtggaatata agtggttttc ttatgttccg caaaggaatg gtacaccaaa cgaagtgcgg
     4141 tagggatttt tgaatctcta caaagaaagg acgtgaatat atggacgata tgcaagtcta
     4201 tattgcgaat ttaggcaaat acaatgaggg cgaattggtc ggtgcgtggt ttacctttcc
     4261 cattgacttt gaggaagtca aagagaaaat cggcttgaat gatgaatatg aggaatacgc
     4321 cattcatgac tacgagttac cctttacggt tgacgaatac acttccattg gcgaactcaa
     4381 tcgactatgg gaaatggtat cggaattacc cgaagaatta caatcggagc tatctgctct
     4441 gctcactcat ttttcaagca ttgaagaact aagcgaacat caagaggata ttatcattca
     4501 ttccgattgt gatgatatgt atgacgtggc acgctactac attgaagaaa cgggtgcttt
     4561 aggcgaagta ccagctagtc ttcaaaacta tattgattat caagcctatg gtcgggattt
     4621 agacctttca ggaacgttta tctcaaccaa tcatgggatt tttgaaatcg tctattaaat
     4681 ctgtcggtac attactactg gcagattttc tattttacgg ggtggctcaa tcagctaccc
     4741 ctatttttta tgaaaggatt gattacatga agaaaatacg aagctatacc agtatctggt
     4801 ctgtggaaaa ggtactgtat tctatcaatg attttagact tccgtttccc ataaccttta
     4861 cgcaaatgac atggtttgtc gtgtcactct ttgcagtgat gatacttggc aacttgcccc
     4921 ctctttccat gatagaggga gcatttctca aatactttgg gattcctgtg gctttcacat
     4981 ggtttatgtc tacaaaaact tttgatggta aaaagcctta tggatttttg aagtctgtca
     5041 ttgcttatgc actgcgacca aagctgacct atgcaggaaa aaaagtaacg cttggcagaa
     5101 accagccaca agaagccatt acagcagtta ggagtgaatt ttatggcata tccaattaaa
     5161 tacattgaaa acaatctcgt ctggaataaa gacggggaat gttatgctta ctatgagctt
     5221 gttccttaca attactcatt tctaagtcca gaacagaaaa tacaagtgca tgattctttc
     5281 agacagctta tcgcacaaaa tcgtgatggc aaaattcatg ctttacaaat cagtacagaa
     5341 tccagcatac gttctgcaca agagcgttcc aaaaatgaag tcactggcaa gctcaaagcg
     5401 gttgcctatg acaaaatcga ccaacagaca gacgctttaa tatccatgat tggcgaaaat
     5461 caagtgaact accgtttctt tatcggcttt aagttgcttc tcaacgatca ggagttttct
     5521 atgaaaagtc ttaccgttga agcaaaaaat gctttgtctg attttgtcta tgatgtgaac
     5581 cataagctga tgggcgattt tgttagtatg agtaatgatg aaatcctgcg ttttcagaag
     5641 atggaaaagc tcttagaaaa taaaatctct cgtcgtttca aaatccgcag gttagataag
     5701 gacgacttcg gctatctgat tgaacacctt tacggacaga caggcactgc ctatgaagag
     5761 tatgagtacc atctatcaaa gaaaaagctg gataatgaaa cgctgattaa atactatgac
     5821 ttgattaagc ctactcgctg tttggtggaa gaaaaacagc gatatttgaa aatccagcag
     5881 gaagatgaaa ccgtctatgt agcttacttt accattaaca gcattgtcgg agaactggac
     5941 ttcccgtcct ctgaaatctt ctactaccag caacagcaat ttacattccc gattgatacg
     6001 tcaatgaatg tggaaattgt agcgaatcgt aaagccctat ctactgtccg caataaaaag
     6061 aaagaactga aagacttgga taaccacgct tggcaaagtg ataatgaaac cagctccaat
     6121 gtggcggaag ctctggaaag tgtgaatgag ctggaaacca atttagacca aagcaaggaa
     6181 tctatgtaca agctgtctta tgtggtaagg gtatcagcaa atgatcttga cgaactcaaa
     6241 cgtcgttgta atgaagtgaa agatttttat gacgatttaa gcgtaaaact ggtacgacca
     6301 tttggggata tgctcggctt acatgaagaa tttttacctg ccagcaagcg ttatatgaat
     6361 gattatattc aatacgtgac ctctgatttc ctcgctggtt taggttttgg tgctactcaa
     6421 atgctggggg aaaatgaggg gatttatgtt ggctacagct tagatactgg acgcaatgtc
     6481 tatctgaaac ctgctcttgc cagtcaaggg gttaagggtt cagtaaccaa tgcgttagcg
     6541 tcggcttttg ttggttcgct gggtggtggt aaatcctttg cgaataacct tatcgtctat
     6601 tatgcggtgc tttatggggc acaagcagtg attgtagacc caaaagcaga acgtggcaga
     6661 tggaaagaaa ccttgccaga gatttcccat gaaatcaata tcgtcactct gacttctgat
     6721 gagaaaaaca aaggcttact tgacccttat gtgattatga aaaatcccaa agattctgaa
     6781 tcactggcta ttgatattct gacattcctt acggggattt cctctcgtga tggggaacgc
     6841 ttcccaatcc ttagaaaagc cattcgtgca gtaaccaata gtgaagtacg agggttgatg
     6901 aaagtgattg aggaattacg ggttgagaat acgccactaa gtaccagtat agccgaccat
     6961 atcgaaagtt ttacagacta tgactttgca catttattat tcagtaatgg ttatgtggag
     7021 cagtctatca gcttagaaaa acaactgaac attatacagg ttgcggactt ggtacttccc
     7081 gacaaggaaa cttcctttga ggaatatacc actatggagc ttttatccgt tgctatgctg
     7141 attgtcatta gtacctttgc tttagacttt atccatacag accgaagcat tttcaagatt
     7201 gtagatttag acgaagcatg gagcttttta caggtagcgc aaggaaaaac actatctatg
     7261 aagctggttc gggctggtcg tgctatgaac gctggggtat atttcgtgac ccaaaataca
     7321 gacgacctct tagatgaaaa actgaaaaat aacctcggct taaaatttgc atttcgttcc
     7381 actgacctta acgagattaa aaagacctta gccttttttg gtgtagaccc agaggacgaa
     7441 aacaatcaga agcgattgcg tgatttggaa aacgggcaat gccttatcag tgatttatat
     7501 ggtcgtgtcg gtgtgataca gttccaccct gtatttgaag aactgctcca tgcctttgat
     7561 accagaccac ctgtgcgaaa agaggtgtaa atgtgaaacc atcaatagta aacagaataa
     7621 aatcaaactg gacgctgaaa cgtctaggta aagtggcaat gacagtggct ttcacacttg
     7681 tgattgccat ttttctttta gccatgctgg gaacggtggt tcaagctgcg ggcttggtag
     7741 atgatacggt caatgtggca aatgaataca gccgataccc acttgaaaac tatcaactgg
     7801 atttttatgt ggataatagc tggggctggc ttccgtggaa ctggtcggac gggattggaa
     7861 aacaggtcat gtatggacta tatgccatta ccaattttat ttggacaatc agtttgtatg
     7921 tttccaatgc gacaggttac ttagtacagg aagcctattc cttagacttc atttccgcta
     7981 cagcagattc cattggtaag aatatgcaga ccttagctgg tgtgagtgca aacggatttt
     8041 caacagaggg tttctatgtt ggattcctct tactcttgat tttggttctt ggggtttatg
     8101 ttgcctatac gggactgata aagagagaaa ccacaaaggc aattcatgcc attatgaatt
     8161 ttgtgctggt gtttatccta tcggcttcct ttattgccta cgctcccgac tacattaaaa
     8221 aaatcaatga cttttcatca gacatcagta atgccagttt atcacttggc acgaagattg
     8281 tcatgcccca ttccgatagt caaggcaagg acagcgtgga cttaatcaga gatagcctgt
     8341 tttccataca ggttcagcaa ccgtggctac tgcttcaata caacagttca gacattgaaa
     8401 gtatcggtat tgaccgtgtg gaaagcctgc tctccaccag cccagattcc aacaatggcg
     8461 aagacagaga aaaaattgtt gcggaagaaa ttgaagacag aagcaatacc aatctaacca
     8521 ttacaaagac cattaaccgt ttaggtacag tcttcttcct atttgtcttc aatattggga
     8581 tttccatatt tgtattccta ttaacaggaa tcatgatttt ctcgcaggta ctttttatca
     8641 tctatgctat gtttctgcct gtgagcttta ttttaagcat gattccatca tttgatggta
     8701 tgtcaaaacg agccataaca aagctcttta ataccatttt gacacgagct ggaatcacat
     8761 tgattattac gacagcattt agtatttcaa ccatgctcta taccttatcg gctggttatc
     8821 cgttcttttt gattgctttt ctacagattg tgacctttgc aggaatctac ttcaagctgg
     8881 gcgatttaat gagtatgttt tctctacaga gtaacgattc tcaaagtgtg ggaagtcgtg
     8941 tgatgagaaa acctcgtatg cttatgcacg ctcacatgca ccgtctacag cggaaacttg
     9001 gacgttccat gactactcta ggggctgggt ctgccattgt tacaggtaaa aaaggacagt
     9061 cgggttcggg gagttctgca aggacacaag cagatcactc ccgaccagac ggaaaggaaa
     9121 aatcaacact tggaaaacgt atcggtcaaa ccatcggtac agtagctgat accaaagaca
     9181 gaatggtaga cactgctagt ggtttgaaag aacaggttaa agatttgccg accaatgcaa
     9241 gatatgcagt atatcaagga aaatccaaag taaaagagaa tgtccgtgat ttaaccagta
     9301 gtatttctca aaccaaagcg gacagagcca gtggacgcaa ggaacagcag gaacaaaggc
     9361 gaaaaaccat tgcgaagcgt cgctctgaaa tggaacaggt caaacagaaa aaacagcctg
     9421 cttcttctgt tcatgaaaga ccgactacaa gacaagaaca atatcatgat gaacagacct
     9481 caaaacagtc taatattcag acttcatata aggaatctca acaagccaaa caagagcgtc
     9541 cagcagttaa gtccgatttt tcaagtccaa aagtggaacg ccaaggcaat accgttcaag
     9601 aaaaaaccgt tcaaaagcca gcaacttcaa ccactacagc agatagaact tcacaacgtc
     9661 caatcacaaa agaacgtccg tctactgttc aaagagtacc actacaaaat acaagaagta
     9721 gaccaccaat caaaaccgcc accattaaga aagtcggtaa gaaaccatga agttgaaaac
     9781 tttagtgatt ggtggttctg gattattctt gatggtcttc tcactgcttc tgtttgttgc
     9841 cattttattt tcagatgaac aggacagcgg aatttccaat attcattatg gaggtgtgaa
     9901 tgtttccgca gaagtgctgg ctcataagcc tatggtagaa aaatatgcca aagaatatgg
     9961 cgttgaagaa tatgtcaaca tacttcttgc gattatacag gtggaatcgg gcggtactgc
    10021 ggaagatgtt atgcagtcct cggaatccct cggtcttcca cctaattcat tgagtacaga
    10081 agaatccatt aagcaaggtg tgaagtattt cagtgaatta ttagccagta gcgaaaggct
    10141 cagtgtagat ttagaatcgg ttatccagtc ctacaattat ggtggtggtt tcttagggta
    10201 tgtggctaat cgtggaaata aatatacctt tgaactggct caaagtttct caaaagagta
    10261 ttcaggtggc gaaaaagtgt cttaccccaa tcccatagcc atacctatca atgggggctg
    10321 gcgatacaac tatggcaata tgttttatgt gcaactggta acgcagtatc ttgtcacaac
    10381 agagtttgat gatgatacgg tacaagccat catggacgaa gcactgaaat atgagggctg
    10441 gcgatacgtt tacggtggag cttccccgac tacttctttt gattgtagcg gactgacaca
    10501 atggacgtat ggaaaagctg gaattaactt accacgaacc gcacaacagc aatatgatgt
    10561 gacccagcat atcccactat cggaagcaca agctggcgat ttggttttct ttcattctac
    10621 ctataacgct ggctcttata ttactcatgt tgggatatac cttggcaata accgtatgtt
    10681 tcatgcaggc gacccaatcg gttatgccga cttaacaagc ccctactggc aacagcattt
    10741 agtgggagca ggacgaatca aacaatgaga aaggaagatt taatgatgaa atttagaaaa
    10801 aatcagaata aagaaaaaca gataccaaag gaaaagaaac ctcgtgtcta taaggtcaat
    10861 cctcataaaa aggttgtgat tgccttgtgg gtacttttag ggcttagttt cagctttgcg
    10921 atattcaagc actttacagc tatagatact catactattc acgaaacaac tatcatagaa
    10981 aaggaatacg ttgatactca tcatgtagaa aattttgtag agaactttgc gaaagtctac
    11041 tattcatggg agcaatccga taagtccatt gataatcgaa tggaaagtct aaaaggctat
    11101 ctgacagatg aacttcaagc tctcaatgtt gatacagtac gcaaagatat tcctgtatcg
    11161 tcttctgtaa gaggatttca gatatggacg gtagagccaa ctggcgacaa tgagtttaat
    11221 gtaacctaca gtgtagacca gctcattaca gagggagaaa atacaaagac cgtccactct
    11281 gcttatatag tgagtgtcta tgtagatggt tctggaaata tggtactggt taagaatccg
    11341 accattacca acatacctaa gaaatcaagt tataaaccaa aagccattga aagtgagggg
    11401 acggttgatt ccattacaac caatgaaatc aatgagtttt taacgacgtt cttcaagctc
    11461 tatcctacag cgacagccag tgaactttcc tactatgtga atgacgggat attaaaacca
    11521 atcggaaaag agtacatctt tcaagaactg gtaaatccta ttcacaatcg taaggataat
    11581 caagtcacgg tatcgctgac agtggagtat atcgaccagc agaccaaagc aacgcaggta
    11641 tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat agaataacaa
    11701 atattggtac attattacag ctattttgta atcacgtact ctctttgata aaaaattgga
    11761 gattccttta caaatatgct cttacgtgct attatttaag tatctattta aaaggagtta
    11821 ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg aacaaataat
    11881 tggatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca
    11941 tgtgattcta aagtatccgg agaatatctg tatgctttgt atgcctatgg ttatgcataa
    12001 aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat
    12061 ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa
    12121 aactacctta acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt
    12181 ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat
    12241 tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc
    12301 aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat
    12361 tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact
    12421 taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa atggaattga
    12481 tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca
    12541 gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga
    12601 tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga
    12661 agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct
    12721 ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa
    12781 taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat
    12841 tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca
    12901 tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac
    12961 ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt
    13021 gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag
    13081 aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga
    13141 acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg
    13201 atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat
    13261 ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc
    13321 tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt
    13381 gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag
    13441 tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcgt ttcaaaatgc
    13501 agttatggag gggatacgct atggctgtga acaaggattg tatggttgga atgtgacgga
    13561 ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt
    13621 tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt
    13681 agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa
    13741 cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat
    13801 tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt
    13861 tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga
    13921 acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa
    13981 taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg
    14041 aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact
    14101 acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata
    14161 ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat ttaattgaaa
    14221 taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag agccgataaa
    14281 atgagattaa tacaatctca ttttatcggc tctttccgtt atgtatggat tcttttaatt
    14341 agtcttcgat gtttcttgct tcgttgatac cgctggctaa agattccatt aaggatagtt
    14401 ctttgtctgt aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg ctttttacca
    14461 agttattagc aggtaagaaa aattcatcaa cggaaacatg aagtaacgat acaaggtcat
    14521 aaagaacttg tatgctgggg tgttgccctt tattttcaat attagttaag taccgtgggt
    14581 caatttcaat caatgctccc acttgttcac gagttaaacc tcgtttcaat cgagcttctt
    14641 taatggctaa accaaaggct ctaaaatcat atttatcttc ttttttacgc atagtagacc
    14701 acctctatac attttattgt tcctactgaa ttaaaaacag gtatagaaaa acgtgttata
    14761 tggtttatag gtttatattt aataaaaagc actactaaac gccaataaaa aaaaccgtta
    14821 tatggtagtg ctatttacgc tgttaaaata ttgtatatta cttccaaatg gcggtttgtt
    14881 ggaggtcaac gtcgccatga agtacatcat atacaataaa tttccttaca ttgggttctt
    14941 gtcaaaaaaa gtcgtctatc tgcaatagat aagtacgtcc accaatgtgg ttttataaat
    15001 catatagata gaataacaga agcatgtaaa cagagaaata aatctgttta tatgcttttt
    15061 tggctattca gaactttttt acaaagttta tttatcagta atgcaacaaa tccccctttc
    15121 acattgggac taagagtgaa aggagataaa cgagcaaggc tcacttcctt tcctagacag
    15181 aaagggggtg agaaacatga aaccatcttc ttttcagacc acaatagaaa atcagtttga
    15241 ctatatctgt aaacgtgcta tggaagacga gcgaaagaat tatatgcttt atctttcaag
    15301 gattgcaaag cgtgaggtgt ccttttcgga tgttggcgat tatcttgtta gccagtttgc
    15361 gacaacagat aactattcaa ctgactttca gatttttaca ctcaatgggt tatcagtagg
    15421 cgttgaaaat gatttgttga gtgaagcatt acgtgagttg ccagacaaga aacgtgaaat
    15481 tctactgctg ttttacttta tggacatgag cgattcagaa attgcagacc tgttgaaatt
    15541 gaaccgttct actgtctatc ggcatagaac cagtggacta gccttaatta aaaagtttat
    15601 ggaggaattt gaagaatgaa aacacaatat cctatgattc cctttcctct cattgtaaag
    15661 gcaacagatg gcgataccga agcgattaac cagattctac atcattacag agggtacata
    15721 acgaagcgtt ccctacgact tatgaaagat gaatatggca atcaaagtat ggtcgttgat
    15781 gaagtcttac gtggaagaat ggaaaccaga ctgattacaa agattttgtc atttgaaatt
    15841 aagtaatatc ctctctcctt tcgtggaagc gtgctaaacc attccacgct tcccgaacag
    15901 ggaggtttgt tattccacca aagcatattg agctttcaat gtgttttgat aggctaacga
    15961 gccattgttc tttgaaaact gaataaaagt aatcgaatac gtttcgataa gaaaagagcc
    16021 aacggaacta accgccatga cctatcttat aaagatagcg agcgattcat gttagtgatc
    16081 cgagaagcaa tctttagcag gattgcctgc aacgacattc ttatcgtgat aatgatactc
    16141 ccatacagtc aatagtccga gcgtgataaa accgtcgcag gcaatgagta tggctacatg
    16201 agaaccatgc aggggtggaa ctcccgtgag ctttgctaaa gctgttcgat tgctggtaaa
    16261 acaactttta tgaaatccaa ataagtgatt tggaaaggag gattttatga agcagactga
    16321 cattcctatt tgggaacgtt ataccctaac cattgaagaa gcgtcaaaat attttcgtat
    16381 tggcgaaaac aagctacgac gcttggcaga ggaaaataaa aatgcaaatt ggctgattat
    16441 gaatggcaat cgtattcaga ttaaacgaaa acaatttgaa aaaattatag atacattgga
    16501 cgcaatctag cgtcgccaaa gggtcttgta tatgataaaa tagtattaag tcgtatcaag
    16561 gctctttcca taaaggaaag gagcaaatgc catgtcagaa aaaagacgtg acaa
//
